LOCUS       DBMDXF010000001        29504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_548_length_29504_cov_2.390300, whole genome shotgun sequence.
ACCESSION   DBMDXF010000001 DBMDXF010000000
VERSION     DBMDXF010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 29504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29504
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_548_length_29504_cov_2.390300"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            58..714
                     /locus_tag="ACZPD4_00005"
     CDS             58..714
                     /locus_tag="ACZPD4_00005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1377493.1"
                     /translation="MEELNCKTVLKLPVLGGISESVVVTWIIMAVLVLLSIILVRNLK
                     VENPGKVQLALESMIGWAQDFFEGIIGKENKAYVPYLITVLLYLAVSNTIGLLGFKPP
                     TKDLNVTAALAIMSMCVIEFSGIHKNGVVHWMKHFAKPVPVVAPITILEIVIRPLSLC
                     MRLFGNMLAGFVVLELLKYFVPLICPIPVSFYFDIFDGLLQAYVFVFLTALFMTEEME
                     "
     gene            826..1050
                     /gene="atpE"
                     /locus_tag="ACZPD4_00010"
     CDS             826..1050
                     /gene="atpE"
                     /locus_tag="ACZPD4_00010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1377494.1"
                     /translation="MEAIIAIGAAVAVLTGIGAGVGIGIATGKAAEAIARQPEAESKI
                     SKNLILGCALAEATAIYGFIIGILIIILLG"
     gene            1101..1601
                     /gene="atpF"
                     /locus_tag="ACZPD4_00015"
     CDS             1101..1601
                     /gene="atpF"
                     /locus_tag="ACZPD4_00015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1377495.1"
                     /translation="MIEINMNLVYTIINVIILYLLLRHFLIKPVTDIMEKRKQLIADG
                     LQSAQDAQDGALKMKQEYEAALNGAKQESVQIVENARKSAKAEYDRILEEAGNKADSM
                     IASAKETIRIEREKTIHELKGEITGLAIASAAKIAGGKSDEERESQLFDQFLRETGEG
                     HGNENK"
     gene            1588..2115
                     /gene="atpH"
                     /locus_tag="ACZPD4_00020"
     CDS             1588..2115
                     /gene="atpH"
                     /locus_tag="ACZPD4_00020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1377496.1"
                     /translation="MKTNRKVICSSSAVRYAETLFELNIPKETIEKTREIFSEVPQIT
                     DVLDNPTIRQEKKEQVIDKVFPREMRNFLKIVCRYRKVRLLGEIFDAYDMRADEEEQI
                     IRAVLFYTALPSEEQKKGMESFLCRKYGAKRAYIEMKKDDSLIGGFILRVGNDEYDRS
                     TKGRLDRLEQRLTRR"
     gene            2121..3626
                     /gene="atpA"
                     /locus_tag="ACZPD4_00025"
     CDS             2121..3626
                     /gene="atpA"
                     /locus_tag="ACZPD4_00025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1377497.1"
                     /translation="MSSINSNEIISILKEEIENFDMMSKDSEVGTVITVGDGIASIYG
                     IDHAMYGEIVTFENGLKGMVQDIRRNEIGCILFGSDTRISEGTKVTRTGKRAGVPVGD
                     AYIGRVVNALGEPIDGKGSIEADGYRPIEQEAPGIVDRKSVSVPMETGILSIDSMFPI
                     GRGQRELIIGDRQTGKTSIALDTILNQKDKDVICVYVAIGQKASTVAKVVNTLKTNGA
                     LDYTIVVSSTASDCAPLQYIAPYAGTAMAEYFMYKGKDVLIVYDDLSKHAVAYRALSL
                     LLGRSPGREAYPGDVFYLHSRLLERSSRLSDENGGGSITALPIIETQAGDVSAYIPTN
                     VISITDGQIFLESGLFAAGMRPAVNVGLSVSRVGGAAQTKAMKKASGSIRIDLAQYRE
                     MEVFTQFSSDLDPATKEQLEYGSGLMELLKQPLYHPMSLHEKVITLCAATHKIFLGID
                     KKEIRQFRLDLMTWFDTKYPNIGEEIEEKKVLSDELIDQIVLIAEEFKKSR"
     gene            3631..4515
                     /gene="atpG"
                     /locus_tag="ACZPD4_00030"
     CDS             3631..4515
                     /gene="atpG"
                     /locus_tag="ACZPD4_00030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1377498.1"
                     /translation="MANIREIQSRINSVKDTMKITNAMYMISSSKMTHAKKKLADTEP
                     YFYGLQGEISRILRHLPDLHHGYFSARENVPEGERKIGSIVITADKGLAGAYNHNIVK
                     LEEEILARPGIHKLFIVGELGRHYFAKRDVQIDTNFQYTVQNPTMHRARDIAAVLLDQ
                     FRERELDEIYIVYTRMENSVQAEAEVMKLLPLERSSFNEMKMPLNVHREAIALYPSAE
                     AVMDSIIPNYVTGIVYGALVEAYASEHNARMMAMKSATDSAKSLIHDLSIIYNRARQA
                     AITQEITEVCSGARAQRK"
     gene            4615..6003
                     /gene="atpD"
                     /locus_tag="ACZPD4_00035"
     CDS             4615..6003
                     /gene="atpD"
                     /locus_tag="ACZPD4_00035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1377499.1"
                     /translation="MNKGKIIQVMGPVVDVVFQDGNLPFIKDALEVENNGKKCVMEVA
                     QHLGNNEVRCLMLAASEGLHKDMEVTATGAGIKVPVGEKTLGRLFNVLGETVDDGEQI
                     TDADQWVIHRDPPTFEDQSPVVEILETGIKVIDLLAPYAKGGKIGLFGGAGVGKTVLI
                     QELIRNIATEHGGYSIFTGVGERSREGNDLWTEMKESGVLEKTALVFGQMNEPPGARM
                     RVAETGLTMAEYFRDEEHRNVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATE
                     MGELQERIASTKNGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKVVEQGIYP
                     AVDPLESSSRILEAEIVGEEHYEVANKVIAILQKYKELQDIIAILGMEELSDEDKATV
                     MRARKIQKFLSQPFFVAETFTGVPGKYVPLKETIRGFKMIIDGEMDEYPESAFFNVGS
                     IDDVIEKAKAAE"
     gene            6017..6439
                     /gene="atpC"
                     /locus_tag="ACZPD4_00040"
     CDS             6017..6439
                     /gene="atpC"
                     /locus_tag="ACZPD4_00040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1377500.1"
                     /translation="MDTFGLRIIASDKVFFNGRCKKLTIPAPDGEKGVLAHHENMVIA
                     VVVGIARMESTEGEVTEIAVGTGFAEIVNNRVTLLVDTAERPEDIDVRHAREQREHAQ
                     ERMRQKQSIQEYYHTQASLARAMNRLKLAQGAKDLIHY"
     gene            6691..8313
                     /locus_tag="ACZPD4_00045"
     CDS             6691..8313
                     /locus_tag="ACZPD4_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1377501.1"
                     /translation="MSEGYKIRSLSEIVNEILSTVRSYYDAEYAYYIEKDRGDIETIY
                     EWCDENIEWQRDRMKLLPEERQPKWMKTEITDTTARDYSVFLPISEDVTAVLAVVGVR
                     RGGCTIDLMRAIVQYIPQAIALQKMQKQQEYLSYHDDLTGLLNRNSLVHYFDTVDEKK
                     LKSIGALSVDINGLKNFNKEFGRDYGDEVVIRVGEVLEEYFHSGEAYRLTGDEYLVLV
                     ENTSYQDFTKQVHAVHTKLDNISLGLVSIGYAWEKIDIEVDKLVNNAEVMMREEKRKY
                     YKNLQKGHHEPIIKEDLLQDIENGNFIVCLVPKIDTLTGEVGGAEAVVRYHHKDLGIV
                     DPGRYLNMLEETKLSHYLDLYVFEEVCKTLHKWEVRDIPMIPVSVNFAGATLRQEGLA
                     DKMLHLIEKHHVRCEYLEVEVSESSSDMNQEMLAETSSKIRKGNVRVILDHFGAKDSS
                     FSILSLMEFDGLKLDKSVISNIVGNGRSRIVAQAVIDICRQLGASVQAAGVETQDQFN
                     VLREIGCDYAQGTLFNKPITIETFEVRYLRDR"
     gene            8322..8963
                     /locus_tag="ACZPD4_00050"
     CDS             8322..8963
                     /locus_tag="ACZPD4_00050"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR1377502.1"
                     /translation="MVKGWAADITPLYDKEIYIKYFERLPRFRKEKAMAQADPLKRVQ
                     SVAAWSLWEKLRKKYRLPKETVFNLSHSGEFVMCAVCMGGDLAEGKRIQVGCDIQQIG
                     RAREHLAKRYFCGSEYEAVMSGKTPEERADIFYRYWVLKESFLKATRFGMKLSLDSFE
                     IRLGNPPVLIRKPDLFRQDYYYREYEHPNKLYKMAICSTEKEIAADIDMELKL"
     gene            8960..9757
                     /locus_tag="ACZPD4_00055"
     CDS             8960..9757
                     /locus_tag="ACZPD4_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY"
                     /protein_id="HIR1377503.1"
                     /translation="MKKLTGEFRLRRMLVIGIAIGLVAGVLCTGVAAKSVLSKNKAEF
                     AAEKEKLKKKTNELKEEIMKHEAANNSAEALGSDKEGWALVLVNDSHPLDVSYVPESM
                     VELESERFVDARIEEPLRQMLDDGAKAGLSMYVASAYRSSEKQKEVFNATMQDWMANG
                     RTPLEAYDETKKSVAVPGTSEHATGLAVDIIASEYEGLDDRQGETDEQKWLMEHCWEY
                     GFILRYPPEKVDITGIIYEPWHYRYVGKTAAEEIMKKNLTLEEYLLQ"
     gene            9948..11258
                     /locus_tag="ACZPD4_00060"
     CDS             9948..11258
                     /locus_tag="ACZPD4_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377504.1"
                     /translation="MQDLTFGEQVKIILGRKGMTIKQLAELIEEKTGKKMSRQNLTQR
                     LGRDNFQEQDMRMIAEMLECPFHLSIMSAEGAGEVRSIAALERAAAARVEAVEASGHK
                     ELTAKEQAEAEYAEPEEKLIEETVPEMFEHTEEFAQNKAEAEYIEPEEPVEAEYIQTE
                     EPVEAEYIQTEEPVEAEYIQPEEPVEAEYIQPEESVEAEYIQPEKEADMSMPDTEGLE
                     TPGEEVSNDRDMTIGELYEIHEELNELEKNVQAGESIEEIKKELEKPKKERRLGSFFR
                     RRDSQSKAEKEVSPKENEVQTDAVGESGIGENVNQNDTDHADKAGAVVQDESVSSDAK
                     WTEDAAEDETAFAESGSTEDFEPVITHDDADEDTQAGDVNPYTGKEYQSNSVRMHPTR
                     IGYVQVYDRTIHKWTDMTEWAFLGLQERKKALLGKAYEPPIYLD"
     gene            11453..12859
                     /locus_tag="ACZPD4_00065"
     CDS             11453..12859
                     /locus_tag="ACZPD4_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1377505.1"
                     /translation="MEWSRLLSTKRERAGGNFAKAGDLRSEFEKDYHRIIGSASFRRL
                     QDKTQVFPLDKSDFVRTRLTHSLEVSSLGKSLGQTIGENILEHRKNSGFTPQMKEDIS
                     HILECAGLIHDIGNPPFGHFGEIAIREWFERNLPKLTFCQKNVSEMFTAQMKQDFFHF
                     EGNAQALRLVTRLHYLVDEHGMNLTYALLNTIIKYPGSSLQIDENSGNIKDRKMGYYF
                     ADEEIFHKVTKETGAGRNRHPLTYMLEAADDLAYKTADIEDSFVKGFIRYADLERELK
                     ELEEKKRNGTFRPAYRLRQLYERGIQKRVHDPESYAVKNWIVNAQGFLLNCATEGFMA
                     NYDAIMSGKFGQDLFYGTNGEELMELLGDMADRYVFSSMAIYKMEVSESAVIDYLMER
                     LVQAALYFDTKEKQGTIDRRVISFISDNYKNAYRIQSEGKSEAEKLYLRLLLVTDYVC
                     GMTDSYAKRLYQEMNGII"
     gene            complement(12852..13892)
                     /locus_tag="ACZPD4_00070"
     CDS             complement(12852..13892)
                     /locus_tag="ACZPD4_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
                     /protein_id="HIR1377506.1"
                     /translation="MDTGTFYLYEYENNVRKRNAGFLKIYRRYQSCIFQAHIRSISLE
                     NVFEAEMYAFFFKENQICAKQIAHFPIDSMTHSIRFCAPQSSFPECRRLESIDGFFLK
                     CSESSSLWLTPNCPENIDSDQIHFLDTDSTIQSDSYPASLSTRPDSPQNNSQEKANPL
                     TKSESPDEAEPRAKDESPDETKPPAKDESPDETKPPTKDESPDETKPPTKDESPDEPE
                     PRAKSESNSSIRGEVRQNEISKTGLSLPRKLSLSDLTVLPRKFWFLSNNSFLLHGYHS
                     YGHLLLTEQNGRLWLGVPGIYDLREARAADLFGFPRFCRSYADSDMLTDDERNANADF
                     GHWFRCVGLRPR"
     gene            13996..14484
                     /gene="tadA"
                     /locus_tag="ACZPD4_00075"
     CDS             13996..14484
                     /gene="tadA"
                     /locus_tag="ACZPD4_00075"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR1377507.1"
                     /translation="MEENEKLKLDEKYMRAAIKQAQKAYAINETPIGCVIVYEGKIIG
                     RGYNRRNTDKDPLAHAEIKAIKKASKKMGDWRLEQCTMYVTLEPCQMCAGAIIQSRLT
                     RVVVGCMNPKAGCAGSVLNLLDIKQFNHQAELTTGVLEEECSALMTGFFRELREKNRR
                     KR"
     gene            complement(14645..14906)
                     /gene="ffs"
                     /locus_tag="ACZPD4_00080"
     ncRNA           complement(14645..14906)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPD4_00080"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            15049..16773
                     /locus_tag="ACZPD4_00085"
     CDS             15049..16773
                     /locus_tag="ACZPD4_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR1377508.1"
                     /translation="MKVLLAAVNAKYIHSNLAVHSLKAYARTYTEEIEVAEYTINQTF
                     DAILQDIYRRKPDLLGISCYLWNIEYVGQLLRELPKILPDTKIWLGGPEVSYDAKAVL
                     EQYPKVEGVMCGEGEETFAELLRVYHEEKGVLCSGVKRDELFSDIPGIAYRNKDKAVI
                     QNDFRPVIDLSTIPFVYDHIEDFENRIIYYESSRGCPFSCSYCLSSIDKCLRFREIEL
                     VKKELQFFIDKKVPQVKFVDRTFNCRHDHAMAIWTYIHEHDNGITNFHFEVSADLLNE
                     KEIALINRMRPGLIQLEIGVQSANEITIREINRTMKIDVLSTIVDKIKKGNNVHQHLD
                     LIAGLPYEGYESFGKSFDRVYKMNPEQLQLGFLKVLKGSVMHDRAQEYGIVYQDRAPY
                     EVLSTKWLTYAEVIRLKQIEEMVEVYYNSGQFCCTMAALEQEFASPFCMYECLAAYYD
                     EKNAFAVSHSRIGRYEILYDFIVKTCKERSEQYMEMLTLDMYLRDNVKKRPEFLRESG
                     VSSDEAAAFYKKEEKERTYLKAYEGYDRRQMRKMTHLERIDGKTVLFDYKNRDPLTGN
                     AAIYYLSE"
     gene            16848..17834
                     /locus_tag="ACZPD4_00090"
     CDS             16848..17834
                     /locus_tag="ACZPD4_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR1377509.1"
                     /translation="MKIGKISPTIWNRSVKKKVHMDQTDGSFSWADVCVCGGHAGIGY
                     YAVLGAAGELAARKIQAEGISVRILFPEETEEEFLADVCGEIQKGCECIKAKLTSLSA
                     EGTAAVQTPIVYACAGGTKVQDPGMHALEETADKKTGNRQKFVKNRELLLCGYAGLEG
                     SLRILEAAEEELSQRFINTFLMQAKKLRDQLITPEQILEAAKGRDISVWQIGSGGILA
                     ALWEMAEEEKIGFEIAMGSISLKQETVEICEYYRLNPYQLTSAGSYLILTDEADQVIE
                     VLEKAGARAVRLGVAKAQNARVITSKEEIRYLDRPAPDELARWQKDYKKCEK"
     gene            17931..18434
                     /locus_tag="ACZPD4_00095"
     CDS             17931..18434
                     /locus_tag="ACZPD4_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1377510.1"
                     /translation="MNEKKEALRMEILKYIEKHSRVELGELAVLVGVEETDVANEIAD
                     MEKEKIICGYHTLIDWDKAGVEMVTALIEVRVTPQRDSGFDRIAERLYNYPEVYAVYL
                     ISGSYDLLVTLEGKTLKEVSRFVSERLSTIDGVLSTATYFILKKYKDHGTIMGVKKGS
                     ERIPVTL"
     gene            18434..19606
                     /locus_tag="ACZPD4_00100"
     CDS             18434..19606
                     /locus_tag="ACZPD4_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1377511.1"
                     /translation="MRNPLSRKIVEIQPSGIRKFFDVANEMEDAISLGVGEPDFDTPW
                     RIREEGMFSLEKGRTFYTSNAGLKELRKEICNYLERKIHVKYDAVHETLVTVGGSEAI
                     DVGLRCMLDPGDEVLIPQPSYVSYLPCTVMADGVPVIIPLQEKNEFKLTAEELEKYTT
                     PKTKVLVLPFPNNPTGAIMTREDLEPIAKFVEEHDLYVMSDEIYSELTYKEEHVSIAS
                     LPGMRERTLVINGFSKGFAMTGWRLGYCCGPEVIISQMTKLHQFAIMCAPTNSQYAAV
                     QGLRNCGDEVEEMRKSYNQRRRFLMHEFKKMGLECFEPFGAFYVFPSIKQFGMTSEEF
                     ATRLLNEEKVAVVPGTAFGECGEGFLRISYAYSLEDLKEAIGRLERFIGRLRNERK"
     gene            19609..20112
                     /locus_tag="ACZPD4_00105"
     CDS             19609..20112
                     /locus_tag="ACZPD4_00105"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1377512.1"
                     /translation="MLNIVLYEPEIPANTGNIGRTCVATGTRLHLIEPLGFKLSEKAL
                     KRAGMDYWNDLDVTTYIDYADFLEKNPGAKIYMATTKAKKVYTEAAYEPDCYIMFGKE
                     SAGIPEEILVDHEDTCVRIPMMGQIRSLNLGNSVAVVLYEALRQNGFAKMELEGELHE
                     LEWHGNT"
     gene            20444..20752
                     /locus_tag="ACZPD4_00110"
     CDS             20444..20752
                     /locus_tag="ACZPD4_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377513.1"
                     /translation="MVEETIKSIRETENKADQIVKEAEQESKRILKTAKEEAKQAADK
                     LIDEAKSDALKTANQAKKDGEVMLAQAAEETRREAEQMKKAALERKKEAAALVLERLT
                     "
     gene            20785..22806
                     /locus_tag="ACZPD4_00115"
     CDS             20785..22806
                     /locus_tag="ACZPD4_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1377514.1"
                     /translation="MAVLKMQRISICALKKDRKAILEKLQSLGVVEINHVIEEDEDFK
                     KMDTAGQKAGFEKASTLAEQAIDILDKYVPEKKSMFASLEGKALIEREEEERIRNERR
                     DLLKTAKQIGRLDKERSEQMAEITKLNNSIESLNPWISLDVPLTPAETDRTAVFFGTM
                     PGGTDAETVYGILAEKAPDITGADVNIVSSEPGMVYLAVVCMKEDVQAAEDALRSAGF
                     ARPSQMWGDVPKFQKEELESEIADCRARIEQIENEVKDLSDQREKLKILADYYRVRAD
                     KYAVLGQIPQSERTFVISGYIPQCEAAHLSGYLTEKYDCMVDVEELQENEEAPVILKN
                     NPFSASVEGVVESYGLPHKGELDPTTIMSFFYVFFFGMMLSDAAYGAIVAIVCAVLVK
                     KFPRMSQGMKKSMKLFFYCGLSTLVWGILFGGYFGNIVDVVSEKFFGTTITVPALWFV
                     PLNDPMKLLVFSLLFGVIHLFVGLGIKGYLCLKDGKVMDFFCDVVLWFMLLIGLILML
                     LPSDIFASIAQMNIVFPAWLNTGAKALAIAGAVGIVLMSGREKKNPALRLALGAYDLY
                     NVSGWLSDVLSYSRLLALGLATGVIASVINQMGSMLPNNVIGVIAFVVIFIAGHTLNL
                     AINLLGAYVHTNRLQFVEFFGKFYEGGGEPFNPFKENTKYADVKEETLS"
     gene            22803..23288
                     /locus_tag="ACZPD4_00120"
     CDS             22803..23288
                     /locus_tag="ACZPD4_00120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HIR1377515.1"
                     /translation="MNIAENLGIVYALLGAAVAVLFAGAGSAIGVGIAGQAAAGVVTE
                     DPSKFAKVLIIQLLPGTQGLYGLLVGFITLSKIGILGGGALDVSVSSGLLILAACLPI
                     GIVGLLSGIAQGKTAASSIGIVAKKPEQFGKAMLFPAMVETYAILALLISFLAVNGIQ
                     I"
     gene            23335..23928
                     /locus_tag="ACZPD4_00125"
     CDS             23335..23928
                     /locus_tag="ACZPD4_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR1377516.1"
                     /translation="MSGLDKMKSRIFEEAEQSAAELLDQAKKDAEKIVKDAVEKAGAD
                     AEHIRVKAAADAKEYAKRAESSADMNRKQALLAAKQDVIRSVLEDAYSQVMNMDDAAY
                     FEMLGKMLDKYMLAQDGAICFSKRDLDRMPKSFAEKIDASAKAKGGKLVISEEARKID
                     GGFILVYGGIEENCTIKAVFDAKREELADVVKRQLFS"
     gene            24003..24974
                     /locus_tag="ACZPD4_00130"
     CDS             24003..24974
                     /locus_tag="ACZPD4_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000220 - vacuolar proton-transporting
                     V-type ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V0D/AC39 family V-type ATPase subunit"
                     /protein_id="HIR1377517.1"
                     /translation="MGNTKYTYAVARIRALEVALFSDAVIEQLLSCKTAAEALQFVVE
                     KGWGDMADQDDPDVVLRREEEKTWEVIKEVAPDASVFNVLSYPKLYHNLKAAIKEVCT
                     EARNPNIFYDDCPIPGEEMLRIVENREFSRLPSHMGNVAQEALETLLHTRDGQLCDIM
                     IDKAALEAIRKAGKESGEPIIENYADTMVAIADIKIAVRSRKTGKSADFMRSAMAECD
                     GVNVEQLIRAALAGEDEIAQYLEGTAYAKGAQALRESASAFERWCDNRMIEMLKPQKY
                     EAFSVGPLLGYLIARETEIKTVRIILTGKQNGFSDEAIRERIREMYV"
     gene            24967..25290
                     /locus_tag="ACZPD4_00135"
     CDS             24967..25290
                     /locus_tag="ACZPD4_00135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1377518.1"
                     /translation="MYKIAVIGDYDSIYGFATLGLSICPVKTREDARKKLKQLADGQY
                     GIIYITEAVAAQIEEEIEQYRENTLPAIIQIPGVSGNTGAGVNGVKKTVEQAVGSDIL
                     FGGVS"
     gene            25290..27056
                     /locus_tag="ACZPD4_00140"
     CDS             25290..27056
                     /locus_tag="ACZPD4_00140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1377519.1"
                     /translation="MSTGVIKKVAGPLVIAEGMRDANMFDVVRVSNQRLIGEIIEMHG
                     DEASIQVYEETSGLGPGEPVESMEVPMSVELGPGLITSIYDGIQRPLDDIMKVSGNSL
                     KRGVEVPSLKRNLKWEFVPTAKVGDEVETGDVIGTVQETVLVQQKIMVPYGVKGTIKE
                     IKAGEFTVEEVVAVVETENGDRELTLMQKWPVRRGRPYKKKLPPKMPLVTGQRVIDTF
                     FPIAKGGVAAVPGPFGSGKTVIQHQLAKWAEADIVVYIGCGERGNEMTDVLNEFPELK
                     DPKTGQALMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRWA
                     EALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGHVISLGKDGREGALSVIGAVSPP
                     GGDTSEPVSQATLRIVKVFWGLDSALAYKRHFPAINWLNSYSLYLDDMEKWFNGNVAE
                     DWMEGRQKMMTLLQEEAELEEIVKMVGMDALSPTDRLKMEAARSIREDFLHQNSFHEV
                     DTYTSLKKQHMMMRLVNAFYERSVAALGEGASLRKLISMPVREQIGRFKYVKEDALDA
                     EFVKVDEELSAQIANAFVKEDR"
     gene            27057..28427
                     /locus_tag="ACZPD4_00145"
     CDS             27057..28427
                     /locus_tag="ACZPD4_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1377520.1"
                     /translation="MPKEYRTIQEVAGPLMLVRGVEGVTYDELGEIELASGETRRCKV
                     LEVNGSDVLVQLFESATGINLSNSKVRFLGRSMELGVSEDMLGRVFDGLGRPIDDGPE
                     ILPDARMDINGLPMNPAARCYPEEFIQTGVSAIDGLNTLVRGQKLPIFSASGLPHAQL
                     AAQIARQAKVLGTDENFAVVFAAMGITFEESNFFVESFRETGALDRTVLFINLANDPA
                     IERIATPKMALTAAEYLAFEKEMHVLVILTDITNYADALREVSAARKEVPGRRGYPGY
                     MYTDLASIYERAGRQNGKNGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRDLY
                     RKGVTPPIDVLPSLSRLKDKGIGEGKTRADHSNTMNQLFSAYARGKDAKELMVILGEA
                     ALTEIDLMYAKFADAFEAEYVSQGYNADRSIEETLDIGWKLLRMLPRTELKRIDDKYL
                     DQYYAE"
     gene            28581..29237
                     /locus_tag="ACZPD4_00150"
     CDS             28581..29237
                     /locus_tag="ACZPD4_00150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1377521.1"
                     /translation="MAAKQVNPTRMELTRLKKKRITAIRGHKLLKDKRDELMRQYLDL
                     VRENMEVRKKVEAGIRSANKNFVIAKAGMSEAALNTALMAPKQEVNLEAGEKNVMSVD
                     IPTFQYKTRTADENDIYSYGFAFTSGDLDGAVKSLADVLPDMIRLAECEKACQLMAAE
                     IEKTRRRVNALEHVIIPETEQSIKYITMKLDENERSTQIRLMKVKDMMLEETHHYSEK
                     "
BASE COUNT         9040 a         5205 c         8169 g         7090 t
ORIGIN      
        1 aaaaaaaaaa gcaaagaaaa aggagggagc aatacgggaa atctgacaga aacattgatg
       61 gaggaactga attgtaaaac ggttttgaaa cttcctgttt taggaggaat ttcggaatcg
      121 gttgttgtca cttggatcat tatggcagtt ttggttcttt tatccattat attagtaaga
      181 aaccttaagg ttgaaaatcc cggaaaggta cagcttgcat tggagtcaat gatcggttgg
      241 gcgcaggatt tctttgaagg aattatcgga aaagaaaata aagcttatgt gccgtatctt
      301 atcacggtac ttctttatct ggcagtgtcg aatacgatcg gattgttagg ttttaaaccg
      361 ccgacaaaag acttgaatgt gactgcggcg ctggcgatca tgagtatgtg cgtgattgaa
      421 ttttcgggaa ttcataaaaa cggagtagtc cactggatga aacattttgc aaagccggtt
      481 ccggttgtgg cgccgattac gatattggaa attgtgatcc gcccattgtc gctttgtatg
      541 cggcttttcg gaaatatgct ggcgggattt gtagtgctgg aacttttgaa atattttgtg
      601 ccgcttattt gcccgatccc cgtaagtttc tattttgata tctttgacgg gcttcttcag
      661 gcatatgtat ttgtatttct gaccgcgtta tttatgacgg aggaaatgga atagaatcaa
      721 aagataaaca gccgggctgt ttcaaatgat ggaaacgatt aagaataaaa aacaatcaat
      781 aaaaaacaat caaacagatt aaaaaattaa aggagaacaa aaaacatgga agcaattata
      841 gcaatcggag cagcagtagc agtattgaca ggtatcggag caggcgtcgg tatcggtatc
      901 gcaacaggaa aagcggcaga agcgatcgca agacagccgg aagcagagag taagatcagt
      961 aagaatctga ttctcggatg tgcgcttgcg gaggcaacgg ctatttatgg atttattatc
     1021 ggaattttga tcatcattct tctcggttaa taaagtcaga tgttacattg tgaaaatgac
     1081 gggaaaggca tgggaaaaag atgatagaaa taaatatgaa tcttgtctat acaataataa
     1141 atgtgataat tctctatctc ttgctgagac attttctgat caagcctgtg acagacatta
     1201 tggagaaacg taaacaactg attgcagacg gactgcagag cgcgcaggat gcgcaggatg
     1261 gcgctcttaa aatgaaacaa gaatatgaag cggcgttaaa cggagcaaaa caggagtctg
     1321 ttcagatcgt agaaaatgca agaaaaagcg caaaggcaga gtatgaccgt attctggaag
     1381 aagcggggaa caaagcggac agcatgatcg cgtcggcaaa agagacaatc cgcattgaac
     1441 gggaaaaaac gatccatgaa ctgaagggcg agatcaccgg tcttgcgatc gcttcggcgg
     1501 cgaagattgc aggcgggaag tcggacgaag aaagagagag tcagttgttc gatcagtttc
     1561 taagagaaac aggagaggga cacggaaatg aaaacaaata gaaaagtaat ttgttcttct
     1621 tcggcagtga gatacgcaga aacgcttttt gaactgaata ttccgaaaga gacgatagaa
     1681 aaaactcgtg aaatattttc ggaagtaccg cagatcacag atgttctgga caatccgacg
     1741 attcgtcaag agaagaagga acaggtcata gataaagtgt ttccgcggga aatgagaaac
     1801 tttctgaaga ttgtctgccg ttatcgtaag gttcgtctgc ttggagaaat ttttgacgca
     1861 tacgatatgc gggcagatga ggaggaacag atcataagag cggttctttt ttacacggcg
     1921 cttccaagtg aagaacaaaa aaaaggcatg gaatcgtttt tgtgcagaaa atacggagca
     1981 aaacgcgcgt atattgagat gaaaaaagat gattctctga taggcggatt tattctgcgg
     2041 gtaggaaacg atgagtatga caggagcacg aaaggccgtc tggacagatt agaacaaaga
     2101 ttaacacgga ggtgagcgag gtgagttcga tcaactcaaa tgaaatcatt tcgatcttaa
     2161 aagaagagat tgagaatttt gatatgatga gcaaagacag tgaagtggga actgtcatta
     2221 cagtaggcga cgggatcgct tcgatttatg gaatagatca tgcgatgtac ggtgagatcg
     2281 ttacgtttga aaacggattg aagggaatgg ttcaggatat ccgaagaaac gagatcggct
     2341 gtattctttt cggaagcgat acaagaatca gtgaaggaac gaaagtaacc cgtacgggaa
     2401 agagagccgg agttccggtt ggagacgctt atataggaag agtcgtaaat gcgcttggcg
     2461 agccgataga cggaaaagga agtatagaag ccgacggtta tcgtccgatt gagcaggaag
     2521 cgcccggaat cgttgacaga aaatctgtca gtgttccgat ggagacggga atcctttcta
     2581 ttgactctat gtttccgatc ggaagagggc aaagagagct gatcatcgga gacagacaga
     2641 cagggaaaac gtcgatcgct cttgatacga ttttgaatca aaaagacaaa gatgtgatct
     2701 gtgtctatgt tgcaatcgga cagaaagcgt ctacggtagc aaaagttgtt aatacattaa
     2761 agacaaacgg cgctcttgat tatacgatcg tcgtgtcatc aacggcaagc gactgcgcgc
     2821 cgcttcagta tattgctcca tatgcgggaa ccgcaatggc agaatacttt atgtataaag
     2881 gaaaagacgt attgatcgtg tatgacgacc tgtcaaaaca tgcggttgca tacagggcgc
     2941 tttccctttt gctcggacgt tctccgggac gagaggctta cccgggagat gtattttatc
     3001 ttcactcaag actgctggaa cgctcaagca gattaagtga tgaaaacggc ggcggttcga
     3061 tcactgcgct gccgatcatc gagacacagg caggagacgt atctgcatat attcctacga
     3121 acgtgatctc gatcacagac ggacagatct ttttggagag cggattgttt gcggcgggca
     3181 tgcgtccggc ggtaaatgtc ggtctttccg tatcgcgtgt aggcggggcg gcacagacga
     3241 aggcgatgaa aaaagcgtcg ggaagtatcc gtatcgatct cgcgcagtat agagagatgg
     3301 aagtgtttac acagtttagc tcggacctgg atcctgcgac gaaggaacag ctggaatatg
     3361 gaagcggact gatggaactt ttgaaacagc cgttatatca tccgatgtca ctgcatgaaa
     3421 aagtaattac tctttgtgcc gcgacacaca aaatattcct cggcatcgat aaaaaagaaa
     3481 tacgtcagtt ccgtcttgat ctcatgacgt ggtttgatac gaaataccca aatatcggcg
     3541 aagagatcga agagaaaaaa gtgttgtctg atgaattgat cgatcagatt gtgttgatcg
     3601 cagaggaatt taaaaagagc aggtgataat atggcaaata tcagagagat acaaagccgt
     3661 atcaacagtg taaaagatac gatgaagatt acgaacgcga tgtatatgat ctcttcgtcg
     3721 aaaatgaccc atgcgaagaa aaaacttgcg gatacagagc cttactttta tggcctgcaa
     3781 ggcgaaatat cgagaatact cagacatttg ccggacttgc atcatggata tttttcggcg
     3841 cgcgagaatg ttccggaagg agagcgcaag atcggttcga tcgttattac ggcggataaa
     3901 ggactggcag gggcgtataa tcataatatc gtgaaattag aagaagagat actggcccgt
     3961 ccggggattc ataaattgtt tatcgtagga gaactcggtc gtcattactt tgcaaagcgg
     4021 gacgtgcaga tcgataccaa ttttcaatat acggtacaaa atccgacgat gcacagggcg
     4081 agggatattg ccgcggttct gctcgaccag ttcagggaga gggaattgga cgagatctat
     4141 attgtttata cgaggatgga aaattctgtt caggcagaag cagaagtaat gaagcttctt
     4201 ccgcttgaaa gaagctcgtt taacgaaatg aaaatgccgt tgaacgtgca cagagaagca
     4261 atcgcgctgt atccgtctgc agaagcggta atggatagta tcattccgaa ttatgtgacc
     4321 ggaatcgtct acggagcgct tgtggaagca tatgcaagcg aacataacgc ccgcatgatg
     4381 gcgatgaaat ctgcgacaga cagtgcgaaa agtctgatcc atgacttgtc gatcatatat
     4441 aacagagcaa ggcaggcggc gatcacacag gaaattacag aagtgtgcag cggggcaagg
     4501 gcacagcgaa aatagaaatg cagcagaaaa aagccggggc tgatacctcg ggaagatcag
     4561 tatgtcaaac aggcggcaga atgccgctat agttctaaga aagtgagaaa gatcatgaat
     4621 aaaggaaaaa tcatacaggt tatgggtcct gttgtagacg ttgtgtttca agacgggaac
     4681 cttcctttta tcaaagacgc tttggaagtt gaaaataacg gaaagaaatg tgttatggaa
     4741 gtggcgcagc atctcggaaa caatgaagtt cgctgtctga tgctggctgc aagcgaaggg
     4801 cttcacaaag acatggaagt aactgcaaca ggagcgggga ttaaagttcc ggtcggagag
     4861 aagacgctgg gaagattgtt taacgtactt ggagagacgg tagatgacgg cgaacagatc
     4921 acagatgcag atcagtgggt gattcacaga gatcctccga catttgaaga ccaaagcccg
     4981 gttgttgaga tcctggaaac gggaatcaaa gtaatcgatc ttcttgctcc ttatgcaaaa
     5041 gggggaaaga tcggactgtt tggcggcgcc ggagtaggaa aaacggtgct tattcaggag
     5101 ctgatccgaa acatagcgac agagcatggc ggatactcca tattcacagg agtaggagag
     5161 cgttcccgtg aaggaaatga cttgtggaca gagatgaaag aatcgggagt tcttgaaaag
     5221 acggcgttag tgttcggtca gatgaacgag ccgccgggag cgcgtatgcg cgtggcggaa
     5281 acagggctta ctatggcaga atatttccgc gatgaagaac acagaaatgt tttgttgttt
     5341 attgataata ttttccgttt tacacaggcg ggatcggaag tgtctgcgct tcttggaaga
     5401 atgccgtcgg cggtcggata tcagccgacg cttgctacgg aaatgggaga acttcaggag
     5461 aggatcgcgt caacgaaaaa cggttcggtt acttccgtac aggcggtata cgttcctgcc
     5521 gacgacttga cagacccggc tccggcaacg acgtttgctc atttggatgc gacgacggtt
     5581 ctctcgagaa aagtggtaga gcaaggtatc tatccggcag ttgatcctct ggagtcaagt
     5641 tcccgtattt tggaagcgga gatcgttggg gaagagcatt acgaagtggc gaacaaagtg
     5701 atcgcgattc ttcagaaata taaagaactg caggatatta tcgcaatcct cggtatggaa
     5761 gagttgtcgg atgaagataa agcaaccgtt atgcgtgcga gaaaaatcca gaagtttttg
     5821 tcgcagccgt tctttgtggc tgagacgttt accggagttc cgggaaaata tgtgccgctg
     5881 aaagagacga tccgaggatt taagatgatc atcgacggag agatggacga gtatcctgag
     5941 tcggcatttt tcaatgtagg ttcaatcgac gatgtgatcg agaaagcaaa ggcggcggag
     6001 taggcagagg tgtattatgg atacatttgg attgagaatc atagcaagtg ataaagtatt
     6061 tttcaatgga agatgtaaga aactgacgat tccggcgccg gacggagaaa aaggcgtact
     6121 ggcgcaccat gaaaatatgg tgatcgccgt tgttgtcggt attgcgcgca tggaatcaac
     6181 agaaggtgaa gtgacggaaa tcgcagtcgg aacaggtttt gcggagatcg tgaataaccg
     6241 tgtgacactg ctcgtcgata cggcggaacg cccggaagat attgacgtgc gtcatgccag
     6301 agaacaaagg gaacatgcac aagagcggat gcggcagaaa caaagcattc aggaatatta
     6361 ccacacgcag gcatctcttg ctcgggcgat gaaccgcctg aaacttgcac agggagcgaa
     6421 agacctgatc cactattaag tgtgacagat gcttacggtt gtcgaaagga tacatcgata
     6481 gcatgtttgt gaaaagaaac agataaaaag agtcagatca cgagtgactg gctctttttt
     6541 tatcttgcat tttatatggc tgtatactaa aataataatg cttatgcaag taaattattt
     6601 gcattaaaat gcacagaaaa atgtcagaca gaacagttta aataaatata tggatttaca
     6661 gatcaaagaa aatgaaacgg gcggggaatt atgagtgaag gttacaaaat ccggtctctt
     6721 agcgagatcg taaacgaaat attaagtacg gttcgcagct attatgatgc ggagtatgct
     6781 tattatattg aaaaagacag aggagacatc gagacgatct acgagtggtg cgatgagaat
     6841 atcgagtggc agcgtgatcg gatgaaactt ctcccggaag agcgtcagcc gaaatggatg
     6901 aaaacagaga ttaccgatac gacggctcgg gattatagcg tgtttcttcc gatcagtgaa
     6961 gatgtgactg ctgttcttgc ggttgtcgga gtcagacggg gaggctgcac catcgacttg
     7021 atgcgcgcta tcgttcagta tattccgcag gcaatcgcac tgcagaagat gcagaagcag
     7081 caggaatatc tgagttatca tgacgatctt accggtcttt tgaacagaaa cagtcttgtt
     7141 cactattttg atactgtgga tgagaagaaa ttaaaatcga tcggcgcgct ttccgtcgat
     7201 atcaacggac tgaaaaattt taataaagag ttcgggagag attacggaga cgaagtcgta
     7261 attcgtgtcg gagaagttct ggaggagtat tttcacagtg gggaagcata tcggctgacg
     7321 ggagatgagt atctcgtgct tgtagaaaat acgtcttatc aggattttac aaaacaggtt
     7381 catgcggtgc atacaaagct tgacaatatc agtctcggac ttgtttctat cggctatgcg
     7441 tgggagaaga tcgatatcga ggtagacaag cttgtcaaca acgcggaagt gatgatgcgc
     7501 gaggaaaaaa gaaaatatta caaaaatttg caaaaaggac atcacgaacc gatcataaaa
     7561 gaagatcttt tacaagatat tgaaaacggg aattttatcg tatgtcttgt tccgaagatc
     7621 gatacgctca caggcgaggt cggcggagcg gaagcggttg tgcgctatca tcacaaagat
     7681 ctcggaatag tagatccggg aagatatctt aatatgctgg aagaaacaaa actttcccat
     7741 taccttgatc tttatgtgtt tgaggaagtg tgcaaaacgc ttcataaatg ggaagtaaga
     7801 gatattccga tgatcccggt ttcggtgaat tttgcagggg caactcttcg gcaggaagga
     7861 cttgcggata aaatgctgca tctcatagaa aaacatcatg tcagatgcga gtatctcgaa
     7921 gttgaagttt cggaatcaag cagcgatatg aatcaggaga tgctggcaga gacgagcagt
     7981 aagatacgaa aaggaaatgt aagagtgatc ttggatcatt tcggtgcaaa agattcaagt
     8041 ttttcgatct tgtctcttat ggagttcgac ggattgaaac tggataagag cgtgatttca
     8101 aatattgtcg gaaacggaag gagccggatc gtggcgcagg ctgttatcga catatgcaga
     8161 cagttgggag cgtctgttca ggcagcgggc gtggaaactc aggatcagtt taatgtactc
     8221 agggagatcg gctgtgatta tgcacaggga acattgttta ataaaccgat cacgattgaa
     8281 acgtttgaag tgagatattt aagagacaga taaggaaaag tatggtaaag ggatgggcgg
     8341 cggacattac gccgctttac gataaagaaa tatatataaa atattttgaa cggcttcccc
     8401 gttttcggaa agaaaaggca atggcgcagg cagatccttt aaagagagtg cagagcgtgg
     8461 cggcatggag tctgtgggag aagttgagga agaaatacag acttccgaag gaaacagtgt
     8521 ttaacctgtc acattcaggg gagtttgtca tgtgtgccgt ctgtatggga ggggatttgg
     8581 cagagggaaa acgcatacag gtcgggtgtg atattcagca gatcggcagg gcgcgggagc
     8641 atttggcaaa acggtatttt tgcggttcgg aatatgaggc agtgatgtcg ggaaagacac
     8701 cggaggagag ggcggatata ttttacagat actgggtgct caaagaaagt tttttgaaag
     8761 cgacaagatt cggtatgaag ctttcgcttg actcgtttga gatacgattg ggaaatccgc
     8821 cggtattgat acggaaaccg gatctgtttc ggcaagatta ttattaccgg gaatatgagc
     8881 atccgaataa actatataaa atggcgatct gttctacaga gaaagagatt gccgccgata
     8941 tagatatgga gttgaaatta tgaaaaaatt gaccggagaa ttccggctga gacgaatgtt
     9001 ggtcatcggc atagcgatcg gtcttgtggc gggagttctg tgtacaggag ttgccgcgaa
     9061 gtcggttttg tcaaaaaata aagcggaatt tgccgcagaa aaagaaaaat taaaaaagaa
     9121 aacaaatgag ctgaaagaag agatcatgaa gcatgaggcg gcgaataaca gcgcagaggc
     9181 tttaggaagc gacaaagaag ggtgggcatt ggttcttgtc aacgactctc atcctctgga
     9241 tgtttcttat gtgccggaat ctatggtgga gttggaaagc gaacggtttg tcgatgcgcg
     9301 tatcgaagaa cctcttcggc agatgcttga cgatggggca aaagcgggac ttagtatgta
     9361 tgttgcttcc gcttaccgtt cttctgagaa gcagaaggaa gtgtttaatg caaccatgca
     9421 ggactggatg gcaaacggac gtacgccttt ggaagcttat gatgagacga aaaagtcagt
     9481 ggcagttccg ggaacgagcg aacatgcgac cggacttgca gtggatatta tagcgtccga
     9541 gtatgaaggt ctggatgacc ggcagggaga gacggacgaa caaaaatggc tgatggaaca
     9601 ttgttgggaa tatggattta ttttaagata tcctccggaa aaggtggata tcacgggaat
     9661 catttatgaa ccgtggcatt atcggtatgt agggaaaacg gcggcggaag agatcatgaa
     9721 aaagaatctg acactggaag aatatcttct tcaataaaaa gcctgacagt accgtgcaga
     9781 agaatcgcat aagtataagt ataagcaaca gtccggtccg cgctggttct aaaaaccgca
     9841 ttgcggaaaa tctcttgacg cagactggga aaaagagata ggataaagta tatggggaat
     9901 accgtaaagg gttggtaaag agtataaata tacataagga ggaaaaaatg caggatttaa
     9961 catttgggga acaagtcaag atcattctcg gaaggaaagg aatgacgatc aagcagcttg
    10021 cggaattgat cgaggaaaaa acagggaaga aaatgtcgag acagaacctg acacagcgtc
    10081 ttggcagaga taattttcag gaacaggata tgcgtatgat cgcggaaatg cttgagtgtc
    10141 cgtttcattt gagtatcatg tcggcagaag gggcagggga agtaagaagc attgcggcgt
    10201 tggagcgtgc ggcggcagcg agagttgaag ctgtagaagc ctccggtcat aaagagctga
    10261 cagcaaaaga gcaggcagaa gcggagtatg cagagccgga agaaaaactg atcgaagaga
    10321 ctgtgccgga gatgtttgag catacagaag agtttgcaca aaacaaggca gaagcagagt
    10381 atattgaacc ggaagaacct gtggaagcag agtatattca gacggaagaa cctgtggaag
    10441 cagagtatat tcagacggaa gaacctgtag aggcagagta tatccagccg gaagaacctg
    10501 tggaagcaga gtatatccag ccggaagaat ctgtggaggc agaatatatc cagccggaaa
    10561 aagaagcgga tatgagtatg ccggatacgg aagggttaga gacaccggga gaagaggtat
    10621 cgaacgatcg cgatatgacg atcggcgaat tatacgaaat tcatgaagag ttgaatgaac
    10681 ttgaaaagaa cgtgcaggcg ggcgagtcga tagaagagat taaaaaggaa ctcgaaaagc
    10741 cgaaaaaaga acgcaggctc ggaagttttt tcagaagaag agattcacag tcaaaagcgg
    10801 aaaaagaagt ttcaccaaaa gaaaatgaag tgcagacgga tgcggtcgga gaaagcggta
    10861 tcggagaaaa tgtaaatcaa aacgatacgg atcatgccga caaagcagga gctgtagttc
    10921 aagatgagtc tgtttcgtcg gatgcaaaat ggacagaaga tgcggcagaa gatgaaacgg
    10981 catttgcaga aagcgggagc acggaagatt ttgagccggt cattacacat gatgatgcgg
    11041 atgaggatac tcaggcaggc gatgtgaatc cgtacaccgg aaaagaatac cagtcgaaca
    11101 gcgtgcggat gcatccgaca aggatcggat acgttcaggt gtatgaccgt acgatccata
    11161 aatggacaga tatgacagag tgggcatttc tcggacttca ggaacgtaag aaagctttac
    11221 tcggcaaggc gtatgaaccg ccgatttatc tcgattgacg tttcgtttca attcggcgga
    11281 taattgaaca ataaggaaag tgatacagcc ccgcagagtt ttctgtgggg ttgatttgca
    11341 acggcgatat gtcaaagttc gggcttgcct gagacttggc ggccatttac aacttcggaa
    11401 tgtggatttt ggcgcttccg cctgtttgcg ggaggaaagg agacgataac gtatggaatg
    11461 gagcaggctg ctttcgacaa aacgtgagag agcaggcggg aattttgcaa aggcgggcga
    11521 tctaagaagt gaatttgaaa aagactatca ccgcataatc ggaagtgcat cttttcgccg
    11581 gctgcaggat aagacgcagg ttttccctct tgataaaagc gatttcgtaa ggacgaggct
    11641 gactcattct ctggaagtat cttcgcttgg gaaatcactg gggcagacga taggagaaaa
    11701 tatactggag catcggaaaa actcaggatt tacgcctcag atgaaagagg atatttctca
    11761 tatacttgag tgtgcgggct tgatccatga catcggaaat cctccgttcg gacattttgg
    11821 agaaattgcg atccgggaat ggtttgaaag aaatcttccg aaacttacgt tttgccaaaa
    11881 gaatgtttcc gagatgttta ccgcgcagat gaaacaggat ttttttcact ttgaaggaaa
    11941 tgctcaggcg cttcgtcttg tgacaagact gcactatctt gtcgatgagc atggtatgaa
    12001 tctgacatat gcgctcctga atacgatcat taaatacccc ggatcatctt tgcagataga
    12061 tgagaacagt ggaaatataa aagaccggaa gatgggatat tattttgcgg acgaggaaat
    12121 atttcataaa gtgacaaaag agacaggggc gggaagaaac cggcatccgc tgacatatat
    12181 gttggaagcg gcggatgatc ttgcctataa gacggcggat attgaagatt cttttgtgaa
    12241 aggatttatc cgatatgcgg atctggagcg ggaactaaaa gagttggaag agaagaaacg
    12301 aaacggtacg tttcgacccg catacagact tcggcagctt tacgagaggg gaatacaaaa
    12361 gcgggtgcat gatccggaat cttacgccgt gaaaaattgg attgtaaatg cacagggatt
    12421 tttgcttaac tgtgcgacag aaggatttat ggcgaattac gatgcgatca tgtcgggaaa
    12481 gttcggtcag gatctgtttt acggaacaaa cggggaagaa ctgatggaac tgcttggaga
    12541 catggcggac agatatgttt tttcatcaat ggcaatctac aagatggaag tatctgagtc
    12601 ggctgttata gattatttga tggagcgtct tgtacaggcg gctttgtatt tcgatacaaa
    12661 agaaaagcag ggaacgattg acaggagagt aatatcgttt atatcggata attataagaa
    12721 tgcataccga atccaatcag aaggcaaaag cgaagcggag aaattgtatc tccgcctgct
    12781 gcttgtgacg gattatgtgt gcggtatgac agatagttat gcaaaacgtc tctatcagga
    12841 aatgaacggg atcatctagg ccggagtccg acacagcgaa accaatgacc gaaatcagcg
    12901 tttgcgtttc gctcgtcgtc agtcagcata tcagaatcgg cgtaagatct gcagaagcgt
    12961 gggaaaccga aaaggtctgc cgcgcgggct tctcttaagt catatatgcc gggaactccg
    13021 agccagagcc gtccgttttg ctcggtaagg agaagatgcc cgtaactgtg gtaaccgtgc
    13081 agaagaaaac tgttgtttga aagaaaccaa aatttccgag gaagaactgt cagatcggaa
    13141 agcgatagtt ttcgcggaag agaaagaccg gtttttgaga tctcattttg ccgcacttct
    13201 ccccggatgg aggaatttga ttcgcttttc gcgcgaggtt ctggttcatc cggtgattcg
    13261 tctttcgtgg gaggttttgt ctcatccggt gattcgtctt tcgtgggagg ttttgtctca
    13321 tccggtgatt cgtctttcgc gggaggtttt gtctcatccg gtgattcgtc tttcgcgcga
    13381 ggctccgcct catccggtga ttcgcttttt gtgagaggat tcgccttctc ctgagagttg
    13441 ttctgcgggc tgtccggtct ggtggatagg gaggcgggat atgagtcgct ttgaatcgtg
    13501 gagtcggtat ccagaaaatg aatttgatct gaatctatgt tttcaggaca gttcggcgta
    13561 agccaaaggg aggatgactc ggaacatttc aggaaaaacc cgtcgatcga ttcgagacgg
    13621 cggcattcgg ggaaggaaga ttgcggagcg cagaaacgta tgctgtgtgt catggagtca
    13681 atgggaaaat gagcgatctg ttttgcgcag atttgatttt ccttgaaaaa gaacgcatac
    13741 atttccgcct cgaaaacatt ttcaaggctg atgcttcgga tgtgtgcctg aaaaatacag
    13801 gattgatagc ggcggtagat tttcagaaat cccgcattgc gctttcgtac attgttttca
    13861 tattcatata gataaaaagt acccgtgtcc atcttttacc tcgattcttg tcttttcaat
    13921 ctaatctatt catgaaagaa aagaaaaaga acgtatggga ggaaagaaag cggaggaaca
    13981 tacaggaggg atataatgga agaaaatgag aagttgaagt tagatgaaaa gtatatgcgg
    14041 gcagccatta aacaggcgca aaaagcatat gcgattaatg aaacaccgat cggatgcgtt
    14101 attgtttatg aaggaaagat cataggcaga ggttataaca gaaggaatac agataaagac
    14161 ccgttggcac atgcggagat taaggcaatc aaaaaggcaa gcaaaaaaat gggagactgg
    14221 aggctggagc aatgtacaat gtatgtgacg ttagagccat gtcaaatgtg cgcaggagca
    14281 atcattcagt cgaggctgac ccgcgttgtt gtcggatgca tgaatccaaa agcaggatgt
    14341 gcgggatcgg ttttgaatct tttggatata aaacaattta atcaccaggc agaattgaca
    14401 accggagtgc ttgaggaaga gtgcagtgcg cttatgaccg gatttttcag ggaactgagg
    14461 gaaaagaatc gccggaagcg ctaaaggcac attccgggat tgtaagcggt cgccaaggtc
    14521 tccggcaagt ccggacttta gctcgctgcc attacaaaaa gaaagcccgt gcagacgtta
    14581 cagcagtata gctgtttcgg cacggacttt tcattgagat catataaact ttataaatac
    14641 tttttcgtgc attgtgcttc gagctaggct aacagacgtt acttaggcag ttaactcagt
    14701 tcaggcgccc ttacggcacc cggaaggttc tgcttagtgc tgctccgttc ccgacctgac
    14761 acgattcaca gattcccgtc gcgtaagacc caaacatcaa cgccacttac ctaaggcagc
    14821 tctgccggct gtcgccctct gcacaatatc acccctgcta tagcggattg caggtacaag
    14881 gtaccgctaa caccccggct gcacgagtct taattttaca tggttttaag agaaatgtca
    14941 atgaatagag cgcaaaatca cagtaaaatg taacagttct ttgcggcgga ttatttttag
    15001 tgttattata gagtataaat tttgttggaa aaagagatag gagaaattat gaaggtacta
    15061 ttagcggcag taaatgcaaa atatattcat tcaaatcttg ccgtccacag cttaaaagca
    15121 tatgcgcgaa cttatacgga agaaatcgaa gttgcggaat atacgatcaa tcagacgttt
    15181 gatgcgatct tgcaggatat atataggaga aaaccggacc tattgggaat ttcctgctat
    15241 ttgtggaata ttgaatatgt aggacagctt ttgagagaac tgccgaagat tcttccggat
    15301 acaaaaatct ggcttggagg tcctgaggta tcttacgatg cgaaggctgt gctggaacag
    15361 tatccgaaag tagaaggcgt gatgtgcgga gaaggggaag agacgtttgc ggaactgctc
    15421 cgtgtttatc atgaagaaaa aggggtgctt tgttccggag taaaaagaga tgaactcttt
    15481 tctgatattc cgggaattgc ttatagaaat aaggacaaag ctgtaatcca aaatgatttt
    15541 cgtcctgtta ttgatttgag cacgattccg tttgtctatg atcatattga agattttgag
    15601 aacaggatca tctattatga atcgagcaga ggatgtcctt tttcatgcag ttactgcctt
    15661 tcctctatcg acaaatgttt acgtttccgg gagatagagc tcgtaaaaaa agaacttcag
    15721 ttttttatcg acaagaaagt acctcaggtg aaatttgtgg atcggacgtt taattgccgg
    15781 catgatcatg cgatggcgat ctggacttat attcatgagc atgataacgg aatcacaaat
    15841 tttcattttg aagtttccgc ggatttattg aatgaaaaag agatcgcgtt gatcaatcgt
    15901 atgcgtccgg gtctgatcca gcttgagatc ggtgttcaat cggcaaatga gattacgatc
    15961 cgggaaatca acagaacgat gaaaatcgat gttttgtcaa cgattgttga caaaattaaa
    16021 aaaggaaaca atgtgcatca gcatctcgat cttatcgcag gattgccgta tgaaggatat
    16081 gaaagcttcg gaaaatcatt tgaccgtgta tataaaatga atccggagca gcttcagctt
    16141 ggttttctga aagttttaaa aggatctgtt atgcatgaca gggcgcagga atacgggatt
    16201 gtgtatcagg atcgtgcgcc gtatgaagtc ctttctacaa aatggcttac ttatgcggag
    16261 gtcatccggt taaagcagat agaagagatg gttgaagtgt attataacag cgggcagttt
    16321 tgctgtacga tggcggcatt agagcaagaa tttgcatctc cgttttgtat gtatgaatgt
    16381 ctggcggcat attatgatga aaaaaatgca tttgccgtaa gtcattcacg gatcggaagg
    16441 tatgagatct tatatgattt tatcgtgaaa acgtgtaaag aaagatcgga acaatatatg
    16501 gaaatgttga cattggatat gtatttgagg gataatgtga aaaaacgtcc ggaatttctt
    16561 agagaaagcg gagtttcatc ggatgaagcg gcggcatttt ataaaaaaga agagaaagag
    16621 cggacctatt tgaaagcata tgaaggatac gacagacgtc agatgagaaa aatgactcat
    16681 ttggaacgaa ttgacgggaa aacagtgttg tttgattata aaaaccgaga tccgctcaca
    16741 ggaaacgcgg cgatttatta tctgtcagag taaaggacag gcagacatac tgaaagcggc
    16801 ggagctgtca atttttaaaa taacaatgga aggaaaagca aagaaatatg aagatcggaa
    16861 agatctcacc gactatatgg aaccggtcgg tgaaaaagaa agttcatatg gatcagacgg
    16921 acggaagttt ttcgtgggca gacgtatgtg tttgcggcgg ccatgccggg atagggtatt
    16981 atgcggtttt gggcgcggcg ggagaactgg cagcccgcaa aatacaggca gaagggatat
    17041 cggtaagaat tctttttccg gaagagacag aggaagagtt tcttgcggat gtttgcgggg
    17101 aaatccaaaa aggctgcgag tgcattaaag caaagctgac gtctttgtcg gcagagggaa
    17161 cggcggcggt gcaaactccg atcgtgtacg catgtgcagg cggaacgaaa gtacaagatc
    17221 cggggatgca tgcattagaa gagactgcag ataaaaaaac gggaaacaga cagaagtttg
    17281 ttaaaaatcg agaattgctc ttatgcggat atgcaggtct ggaaggttct cttcgcattt
    17341 tagaagcggc ggaggaagaa ttatcgcaaa gatttattaa cacatttttg atgcaggcaa
    17401 aaaagcttcg tgatcagctc ataacgccgg agcagatttt agaggcggca aaaggaagag
    17461 atatttcggt ttggcagatc ggaagcggag gcatcttggc ggccttatgg gaaatggcag
    17521 aggaagaaaa gatcggtttt gaaattgcaa tgggatcgat ctcgctgaag caggagactg
    17581 tagaaatctg cgaatattat cgtctgaatc cgtatcagct gacttctgcc ggaagttatc
    17641 tgatcttgac cgatgaagcg gatcaggtga ttgaagtgct ggaaaaggcg ggggcgcgag
    17701 ccgtcaggct tggggttgcc aaagcgcaga atgcgcgggt gatcacaagc aaagaagaga
    17761 taagatatct ggacagacct gcgccggatg aactggcgag atggcagaag gattataaaa
    17821 agtgtgaaaa ataataaaaa tacatacaga tgaaaagttt tatccatgat aaaatattat
    17881 tttacaacaa gtaaaacgga aatgatatat aaaggtatag gaggcagaaa atgaacgaga
    17941 aaaaagaagc gttgagaatg gagattttga agtatatcga aaagcacagc cgggtagagc
    18001 ttggagaact ggcagtgtta gtcggtgtgg aagaaacaga tgtggcaaat gagatcgcag
    18061 atatggagaa agagaagatc atctgcggat atcatacgct gatcgactgg gataaagccg
    18121 gcgtagagat ggtgacggcg ctgatcgaag tgcgtgtgac gccgcaaaga gacagcggat
    18181 tcgatcggat cgcggaacgg ttatataact atccggaagt atatgcggtt tatcttattt
    18241 ccggaagtta tgacctgctt gtaactttgg aaggcaagac gctgaaagaa gtatcgagat
    18301 tcgtatctga acgtctgtct acgattgacg gcgttttgag cacggcaact tattttatat
    18361 tgaaaaaata taaagaccac ggaacgatca tgggcgtgaa aaaagggtcg gaaaggatac
    18421 cggtgacact gtaatgagaa atccattatc aagaaaaatc gttgagatac aaccgtccgg
    18481 catccgtaaa ttttttgatg tggcgaacga gatggaagac gcaatttccc tcggagtagg
    18541 agaaccggac tttgatacgc catggcgcat aagagaagaa ggaatgtttt cccttgaaaa
    18601 aggaaggacg ttttatactt ctaatgccgg attaaaagaa ctgagaaaag agatctgtaa
    18661 ttatcttgag agaaagatcc atgtaaaata cgatgcagta catgaaacac ttgtcactgt
    18721 aggcggaagc gaggcaatag atgtcggact tcgctgtatg ctcgaccccg gagatgaagt
    18781 gctcattccg cagccgagtt atgtgtctta tctgccgtgt acggttatgg cagacggagt
    18841 tccggtgatc attcctcttc aggaaaaaaa tgaatttaaa ctgacggcag aagaacttga
    18901 aaaatatacg acgcctaaga caaaggttct cgttcttccg tttccgaaca atccgacagg
    18961 agcgatcatg acgcgggaag atttagaacc gatcgcaaaa tttgtcgaag agcatgatct
    19021 gtatgtcatg tctgacgaga tttattcgga acttacatat aaagaagaac atgtatcgat
    19081 tgcgagcctt ccgggaatga gagagaggac gcttgttatt aacggatttt caaaagggtt
    19141 tgcaatgaca ggctggagac tcggatattg ctgcggaccg gaagttatca tatctcagat
    19201 gacgaagctt catcagtttg caatcatgtg tgcgcctaca aacagccagt atgcggctgt
    19261 gcaaggactt agaaactgtg gggatgaagt agaagaaatg aggaaatcct ataatcagag
    19321 acggcgcttc ctcatgcatg agtttaaaaa gatgggactg gaatgctttg aaccgttcgg
    19381 agcgttttat gttttcccga gtattaaaca atttggaatg acttcagaag aatttgcaac
    19441 caggctgtta aatgaagaga aagtcgcagt tgttccggga acggcgttcg gcgaatgcgg
    19501 cgaaggattt ttgagaatat cctacgcata ttcgttggaa gatctaaaag aagcaatcgg
    19561 ccggctggaa agatttatcg ggcgattgag aaatgagaga aaataattat gctgaatatt
    19621 gtactttatg agccggagat tccggccaat accggaaata tcggaagaac atgtgtggca
    19681 acagggacgc gtcttcatct gatcgagcct cttggattca aattatccga aaaggcattg
    19741 aaacgcgcgg ggatggatta ctggaatgat ctggatgtca cgacttatat tgattatgcg
    19801 gatttccttg agaaaaatcc gggtgccaaa atctatatgg caactacaaa agcgaaaaaa
    19861 gtatataccg aagcggcgta tgaaccggat tgttacatta tgttcggaaa agagagcgca
    19921 ggtataccgg aggagattct tgtagatcat gaagatactt gcgtgcgcat tccgatgatg
    19981 ggacagatca gatctttgaa tcttggaaat tctgtggcgg ttgttctcta tgaagctctg
    20041 cgtcaaaacg gatttgcgaa aatggagttg gaaggagagc ttcatgaatt agaatggcat
    20101 gggaatacct gaacgaaata agttgtgaaa atatgtgcaa aatgacgaaa taatgtaaaa
    20161 aaattcggga aaagtgtagt tcactattga agactttcgg tatataatag tgtcaatgtg
    20221 ctaagaaact gctaagaaac gtttgcggtt acatcggttc ggataaggtg cgggagggcg
    20281 ttttacattc gtttaacgtt gaatctgtac actgtgttac gtctgttcag atagggcaca
    20341 ggaggaggga atcgtttccc gaaaagggaa cgtcccgtat gaagcgggaa agtatggtaa
    20401 aactaaaaag atagaaaaga aagcggaagg tggtgttgcg gaaatggtag aagaaacgat
    20461 caaatctatc cgggaaacag aaaataaggc agatcagatc gtaaaagaag cggaacagga
    20521 aagcaagaga atcctcaaga ccgcgaaaga agaagcaaaa caggctgccg acaagctgat
    20581 tgacgaagca aagtcagacg ctttgaaaac agcaaatcag gcaaagaaag acggagaagt
    20641 tatgctggca caggcggcgg aagaaacgcg cagagaagca gaacagatga aaaaggcggc
    20701 tcttgaacga aagaaagagg cggctgctct tgtgttggaa agattgacat aacagaaata
    20761 agactgtaaa ggagggatag acagatggcg gtacttaaga tgcagagaat cagcatctgc
    20821 gccttaaaga aagaccgaaa agcaatcctc gaaaaattac agagcctcgg tgttgtggag
    20881 atcaatcatg taatcgaaga agatgaagat tttaagaaga tggacactgc agggcaaaag
    20941 gcaggtttcg agaaagcgtc gactctggca gaacaggcga tcgatattct tgataaatat
    21001 gttcctgaga agaagtcgat gtttgcatct ttagaaggaa aggcattgat tgagcgggaa
    21061 gaagaagagc ggattcgcaa tgagcgaaga gatcttctga aaacggcaaa acagatcgga
    21121 aggctggata aagagcgctc tgaacagatg gcggagatta cgaaactgaa caacagtatc
    21181 gagagtctca atccgtggat cagcctggac gttccgctga caccggcaga gacagaccgg
    21241 acggcagtat ttttcggaac gatgccggga ggtactgacg cggagacggt atatgggatt
    21301 ttggcagaga aagcgccgga tataaccgga gcagatgtaa acattgtttc atcagaaccg
    21361 ggcatggtat atctagcagt cgtctgcatg aaagaagatg tgcaggcagc ggaagatgcg
    21421 cttagaagcg ccgggtttgc aagaccttcc cagatgtggg gagacgttcc gaagtttcaa
    21481 aaagaagagc tggaaagtga gatcgcagac tgcagagcgc ggattgaaca gatcgagaat
    21541 gaggtgaaag acctttcgga tcagagagaa aaattaaaaa tattagcgga ttactacaga
    21601 gtacgcgcgg acaaatacgc agtgctcggt cagatcccgc agtcggagag aacgtttgtc
    21661 ataagcggat acattcctca gtgtgaggcg gcgcatcttt ccggttatct gacagaaaaa
    21721 tatgactgta tggtagatgt tgaggaactg caggaaaacg aagaagctcc ggttatttta
    21781 aagaacaatc cgttttcggc aagtgtggag ggcgttgtag agtcttacgg acttcctcac
    21841 aaaggggagc ttgatccaac gacgatcatg tcgtttttct atgtattctt tttcggaatg
    21901 atgctttcgg atgcggcgta tggagcgatt gtggcaatcg tctgcgctgt gcttgtgaag
    21961 aaattcccga gaatgtctca gggaatgaaa aaatcgatga aattattctt ctattgtgga
    22021 ttatccactc ttgtgtgggg aattttattc ggaggatatt tcggaaatat cgtagatgtt
    22081 gtttcggaga agtttttcgg aacgacgatc accgttccgg cgctttggtt tgtgccgttg
    22141 aacgacccga tgaaactgct tgtgttctca ctgttattcg gtgtcatcca cctgtttgtg
    22201 ggactgggga tcaaaggata tctctgtctg aaagacggaa aagtgatgga cttcttctgt
    22261 gacgttgttc tttggtttat gcttttgatc ggtcttatat tgatgctttt gccgagcgat
    22321 atttttgcat ctatcgcaca gatgaatatt gtatttccgg catggctcaa tacaggagcg
    22381 aaggcattgg cgatagcagg agcggtggga atcgtgctta tgtcgggaag agagaagaaa
    22441 aacccggcgc ttcgtctggc gcttggcgca tatgatcttt acaatgtgag cggctggctc
    22501 agcgacgtgt tgtcttactc tcgtctgctg gcgctcggac ttgcaaccgg agtaatcgca
    22561 tcggttatta accagatggg aagcatgctt ccgaataatg tgatcggcgt gatcgcattt
    22621 gtcgtaattt ttatcgcagg tcatacgctc aatctggcga tcaatctttt gggtgcgtat
    22681 gtacacacaa accgtttgca gtttgtggag tttttcggga aattttacga aggcggcgga
    22741 gagccgttta atccttttaa agaaaataca aaatatgcag atgttaagga ggaaacttta
    22801 tcatgaatat tgcggaaaat ttaggaattg tttatgcact gctcggagcg gcagttgcgg
    22861 tattgtttgc cggagcagga tcggcgatcg gagtaggtat tgcaggacag gcagcggcag
    22921 gcgttgtgac agaagatccg agtaagtttg caaaagtgct tattatccag ctccttccgg
    22981 gtacacaggg tctgtatgga ctgcttgtag gatttatcac actttcaaag atcggtattc
    23041 tcggaggcgg agcattggat gtttccgtat cttcaggtct tttgatattg gcagcgtgtc
    23101 ttccgatcgg tatcgtagga cttctttcag gaattgcaca gggaaaaacg gcggcatcgt
    23161 ctatcggaat cgtcgcaaag aaaccggagc agttcggtaa agctatgttg ttcccggcta
    23221 tggttgagac atacgctatt ctggcgctgc tgatctcatt ccttgccgta aacggaattc
    23281 agatttaagg aaaagacgtc tggtttggaa ttgatgaaaa ggagacagcg ataaatgagc
    23341 ggattagata aaatgaaatc ccggattttc gaggaggcag agcagtctgc ggcagaactt
    23401 ctcgatcagg caaaaaaaga tgcggaaaag atcgtgaaag atgcggttga aaaagccggg
    23461 gcggatgcag aacatatccg tgtaaaagct gcggctgacg cgaaagaata tgcaaaacgg
    23521 gcggaatcgt cggctgatat gaacagaaaa caggctcttt tggcggcaaa acaggatgtg
    23581 atccgcagtg tgcttgagga tgcgtacagt caggtgatga acatggatga cgcggcatat
    23641 tttgaaatgc ttggaaaaat gcttgataaa tatatgcttg cccaggatgg agcaatttgc
    23701 ttctctaaaa gagatctcga caggatgccg aagtcatttg cagagaaaat cgatgcatcg
    23761 gcaaaggcaa aaggcggaaa acttgtgatt tccgaagagg cgagaaagat tgacggagga
    23821 tttatccttg tatatggcgg catcgaagaa aattgtacga taaaagcagt ttttgatgcg
    23881 aaaagagaag aactggcaga tgtagtgaag agacagctct tttcgtaaac agtgatctgc
    23941 aatgagcata acaatccgca tttttaatgc ggcggattgt tacagcataa ggaggtttag
    24001 aattggggaa tacaaaatat acttacgcag tcgcacgtat acgtgcgctg gaagtcgccc
    24061 tgttctctga tgcagtcatc gagcagcttt tgtcctgtaa aacggcggcg gaagcgctgc
    24121 agtttgttgt cgagaaaggc tggggtgata tggcggatca ggatgatcct gatgtcgtcc
    24181 tgcggcgtga agaagagaaa acatgggaag tgatcaaaga ggtcgctcct gatgcgagcg
    24241 tatttaatgt tctctcttat ccgaagttgt atcataatct gaaagctgcg atcaaagagg
    24301 tttgtacgga agcgcgtaat ccgaatattt tttatgatga ctgcccgata ccgggagaag
    24361 agatgcttcg gatcgtggag aacagagagt tttcgcgttt gccgtctcat atgggaaatg
    24421 ttgcgcagga agctcttgaa acgcttcttc atacaagaga cggtcagttg tgcgatatta
    24481 tgattgataa ggcggcgctt gaagctattc gcaaagcggg aaaagaatcc ggtgaaccga
    24541 tcattgaaaa ttatgcagat acaatggttg ctattgcgga tatcaagatt gccgtgcgtt
    24601 cccggaaaac gggaaaaagc gctgatttca tgcgaagcgc tatggcggag tgtgatgggg
    24661 tgaatgttga acagctcatc cgcgcggctc ttgccggaga agatgagatc gcacagtatc
    24721 tggagggaac agcgtatgca aaaggcgcac aggcgcttcg agagtcagcc tccgcgtttg
    24781 aacggtggtg cgacaaccgt atgatcgaga tgttgaagcc gcagaagtac gaggcgtttt
    24841 cggtaggacc tctgcttgga tatttgatcg caagagagac ggagatcaag accgtacgga
    24901 ttatactgac aggaaaacag aacggttttt cggacgaggc gatcagggaa aggataaggg
    24961 agatgtatgt ataagatagc agtcatcggc gattacgaca gcatttacgg attcgccaca
    25021 ttaggtctta gcatctgccc tgtaaagacc cgggaagatg ccagaaaaaa attaaaacag
    25081 cttgcagacg gtcagtacgg gattatatat attaccgaag ccgtggctgc acagattgaa
    25141 gaagagatag agcagtaccg tgaaaatacg cttccggcaa ttattcagat accgggcgtg
    25201 tcaggcaata cgggagcggg agtaaacggt gtaaagaaga ccgtggaaca ggcggtcggt
    25261 tcggatattc tgtttggagg agtaagctaa tgagcacagg agtgattaag aaagtcgcag
    25321 gaccacttgt tattgcagag ggaatgcgcg atgcaaatat gtttgacgta gtccgcgtgt
    25381 cgaatcagcg tctgatcgga gagattatcg agatgcacgg agatgaagct tcgattcagg
    25441 tatatgaaga gacttcggga cttggaccgg gagagccggt agagtcaatg gaagtgccga
    25501 tgtccgtaga actcggaccg ggactgatta caagtatata tgacggaata caaagaccgt
    25561 tggacgatat tatgaaagta tcgggaaaca gcttaaagcg cggcgtggaa gttccgtctt
    25621 tgaaacgaaa cctgaaatgg gaatttgttc cgactgcaaa agtcggagat gaagtcgaga
    25681 cgggagacgt gatcggaaca gtgcaggaga cggttctcgt gcagcagaag atcatggttc
    25741 cttatggagt aaagggaaca attaaagaaa tcaaggcagg agagtttaca gtagaagaag
    25801 ttgtggctgt cgttgagacg gaaaacggag acagagaact gacattgatg cagaaatggc
    25861 cggttcgccg tggacgtccg tataaaaaga aacttccgcc aaagatgccg cttgtaacag
    25921 gacagcgagt gatcgatacg tttttcccga ttgcaaaagg cggtgtggca gcagtgccag
    25981 gaccgttcgg aagcggtaag acagtgatcc agcatcagct tgcaaaatgg gcggaagcag
    26041 atatcgttgt gtatatcgga tgcggagagc gtggaaatga gatgacagac gttttgaacg
    26101 agtttccgga attgaaagac ccgaagacag gtcaggcgct gatgcaaaga acagttctga
    26161 ttgccaatac gtctgatatg ccggttgctg cccgtgaagc gtcgatttat acgggaatta
    26221 cgatcgcgga gtatttccgt gacatgggtt attctgtagc gctgatggca gattctacat
    26281 ctcgttgggc agaggcgctt cgagagatgt cgggacgtct tgaagagatg ccgggcgagg
    26341 aaggatatcc ggcttatctg ggaagccgtc tggcacagtt ttatgagcgt gcaggacatg
    26401 tgatctctct cggaaaagac ggaagggaag gagcgctttc cgtaatcggc gcagtatcgc
    26461 ctccgggtgg cgatacgtcc gagccggtat cgcaggcaac gcttcgtatc gtaaaagtat
    26521 tctggggtct tgactcagct cttgcgtata agagacactt cccggcgatc aactggctga
    26581 acagttattc tctgtatctt gacgatatgg aaaaatggtt taacggaaat gtggcggaag
    26641 actggatgga aggacgtcag aaaatgatga cacttctcca ggaagaggca gagctggaag
    26701 aaattgtaaa aatggtcgga atggatgcac tttctccgac agaccgcctg aaaatggaag
    26761 cagccagatc gattcgagag gacttccttc atcagaactc attccatgaa gtagatacgt
    26821 atacctctct gaagaaacag cacatgatga tgagattagt aaatgcattt tatgaaagat
    26881 ctgttgccgc gcttggcgaa ggagcgtctc tccggaaact gatctctatg ccggttcgtg
    26941 agcagatcgg acgttttaaa tatgtgaaag aagatgcatt agatgcggaa tttgtgaaag
    27001 tagacgagga attaagcgct cagattgcaa atgcatttgt aaaggaggac cgctaaatgc
    27061 cgaaagagta tagaaccata caggaagtag ccggaccgct gatgcttgtg cgcggcgtag
    27121 aaggcgtgac ttatgatgag ctcggagaaa tcgagcttgc aagcggagaa acaagaagat
    27181 gtaaagtgtt ggaagtcaac ggaagcgacg tgctcgtcca gttgttcgag agcgcgacgg
    27241 gaatcaacct ttccaacagt aaagtccgtt ttcttggaag aagcatggag cttggcgtgt
    27301 cggaagatat gcttggaaga gtatttgacg gtctcggaag accgattgac gatggcccgg
    27361 aaattttgcc ggatgcccgt atggatatta acggactgcc gatgaatccg gcggccagat
    27421 gctatccgga agagtttatc cagacaggag tttccgctat tgacggactg aatacacttg
    27481 tccgcggtca gaaactgccg atcttctcgg cgtcagggct gcctcacgcg cagcttgcgg
    27541 ctcagatcgc aagacaggct aaggtgcttg gaacagatga aaactttgcc gtagtgtttg
    27601 ccgctatggg tattacgttt gaagagtcga acttcttcgt ggaaagtttc cgcgagacag
    27661 gagcgcttga cagaacagtt ttgtttatta accttgcaaa tgatccggcg atcgaacgta
    27721 tcgcaactcc gaagatggcg cttacagcag cagagtatct tgcctttgaa aaagagatgc
    27781 atgtactcgt tattctgaca gacattacaa attatgcgga tgctctccgt gaagtgtcgg
    27841 ctgctcgtaa ggaagttccg ggacgccgcg gatatccggg atatatgtat acagatttgg
    27901 cttctattta tgaaagagca ggaagacaaa acggtaagaa cggaagtatt acgatgatcc
    27961 cgattctgac gatgccggaa gatgataaaa cacacccgat tcctgacttg acagggtata
    28021 ttacggaagg tcagatcatc ttgagccgtg atctttacag aaaaggagtc acgcctccga
    28081 tcgatgttct tccgtccctt tcccgtctga aagacaaagg aatcggagaa ggcaaaacaa
    28141 gagccgatca ctcgaatacg atgaaccagc ttttctcagc ttatgcacga ggaaaagatg
    28201 caaaagagct gatggtcatt ctgggtgaag cagcgttgac agagatcgac ttgatgtacg
    28261 ccaaatttgc ggacgctttt gaagcggaat atgtatcgca gggatataac gcagacagaa
    28321 gtattgaaga aactcttgat atcggatgga aattgcttag aatgctgccg agaaccgaat
    28381 tgaaacgtat tgatgataaa tatttagatc agtattacgc agagtaagag aatgtatcat
    28441 agttgccgca aggcaatctg cgatacttac aaatcagccg cagagataca gcgcgcaaaa
    28501 gaaagtggag aaatatcttc aaaaaactga gaggaagcat gctgcgatca tctgcggcag
    28561 caggaaaagg aggtagaatt ttggcggcaa aacaagtgaa tccgaccaga atggagctga
    28621 cgcggcttaa aaagaagcgg atcacggcga ttcgtggtca taagcttttg aaagataaac
    28681 gtgacgagct gatgcgtcag taccttgatc tcgtgcggga aaacatggaa gttcgtaaaa
    28741 aagtagaagc gggaatccgc tctgcaaata aaaacttcgt aatcgcaaaa gcaggcatgt
    28801 cagaggcggc tctgaatacg gcgcttatgg ctccgaagca ggaagtgaat ctggaagccg
    28861 gagaaaaaaa cgtcatgagc gtggatattc cgacatttca gtataaaaca agaacggcgg
    28921 atgaaaatga tatttattct tatggttttg cttttacgtc aggagacttg gacggagcag
    28981 taaaatcgct tgcagatgtt cttccggata tgatccgtct tgctgaatgt gagaaagcct
    29041 gccagcttat ggcggcggag atcgagaaga cgagacgccg tgtcaatgca cttgagcatg
    29101 tgatcattcc ggagacggaa caaagtataa aatatattac gatgaagctg gatgagaatg
    29161 aaagaagtac acagattcgc ttaatgaaag tgaaagatat gatgttggaa gaaactcatc
    29221 attacagcga aaaataggat gataaaaaga gagaccaaat gcgaggcgca atcggtttct
    29281 ctttttgcat ttcgtaaagt aaggcattgt ttttaaatga catgtatgct atagtaactc
    29341 tacttacaga aattctttct gtggaagata ccggcagctt cgccggtctg aggaattccc
    29401 tcgaaattac ccgatacaat acaacaacag tttttgcgtg gatagtcttg tagaacagtt
    29461 ctttttttcg tatactgatt tttaagagaa ggtcttaaaa aaga
//
LOCUS       DBMDXF010000002        24542 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_677_length_24542_cov_2.480872, whole genome shotgun sequence.
ACCESSION   DBMDXF010000002 DBMDXF010000000
VERSION     DBMDXF010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 24542)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24542)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24542
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_24542_cov_2.480872"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="ACZPD4_00155"
     CDS             <1..816
                     /locus_tag="ACZPD4_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1377522.1"
                     /translation="MSLTYSYSDPYFNDILGGIQSYCASHNAFISLIVSYSQLHNMTP
                     GLKKQLSELDGLIITEIPPETLEFITTLNKKIVFVDNYIDGYCNIGYNTVYANQLIFD
                     HIIKCGYKKIAYIGGASNSSAPSDFDSCCRMMIFRETLRANNIPYDPELIYNCDWDSK
                     VCAAQVKELLSKHPETEVIFAGSDSLASVILSQLKKLNLKCPQDIGVVGFNDISLSKN
                     FSPALTTVRLPSAEMGKLAAEVLIRQIKSNSVLKQQILLPVELKVRESTRKIR"
     gene            complement(941..2233)
                     /locus_tag="ACZPD4_00160"
     CDS             complement(941..2233)
                     /locus_tag="ACZPD4_00160"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1377523.1"
                     /translation="MEQYIIKGGNPLVGEVEIGGAKNAALAILAAAIMTDETVQIDNL
                     PDVNDVNVMLEAIEGIGAMVQRIDRHTVKINGSTIGDFNIEYDYIKKIRASYYLLGAL
                     LGKYKRAEVALPGGCNIGSRPIDQHLKGFRALGADVDIEHGKIVAEAEKLRGTHLYFD
                     VVTVGATINVMMAAAMSEGLTIMENVAKEPHVVDVANFLNSMGANIRGAGTDVIKIRG
                     VRSLHKTEYSIIPDQIEAGTFMFAAAATKGDVTVLNVIPKHLDATISKLVDIGCEVEE
                     FDDAVRVVAKKRLRCTQVKTLPYPGYPTDMQPQIGVALALAKGTSTITESIFENRFKY
                     LGELARMGAQVKVEGNSATIEGVERFSAARVSAPDLRAGAALCIAGLAAEGITIVDDI
                     VYIQRGYERFEEKLRGLGAMIERVSSEKEIQKFKLKVG"
     gene            complement(2399..2923)
                     /locus_tag="ACZPD4_00165"
     CDS             complement(2399..2923)
                     /locus_tag="ACZPD4_00165"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1377524.1"
                     /translation="MKKNLIFIGMPAVGKSTVGIVVAKRLGMQFIDTDLLIQEQEKKL
                     LCEIIAEVGEDGFIEIENRVNADVDAENAVISPGGSVIYCEEAMKHYKNIGTVVYLKA
                     SYQTIKKRIRNPKKRGVVLRDGQTFRQLYEERVKFFEKYADITICEDGCEIEDTIENV
                     LEEVKNLTMYSEQH"
     gene            complement(3146..3556)
                     /locus_tag="ACZPD4_00170"
     CDS             complement(3146..3556)
                     /locus_tag="ACZPD4_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377525.1"
                     /translation="MNEKLPYYMAYPMPFSFDDERIERMDLEYLKSMYPKIPKRILPY
                     VEEECDRMEYENSMVYDAYPDKLQLKLMCTRVCDNIRRHEHICREEDEKEEDLSEDEA
                     ITMENLRDLVEVMLFQELYKRRCDHRRCCRRFFK"
     gene            complement(3720..4280)
                     /locus_tag="ACZPD4_00175"
     CDS             complement(3720..4280)
                     /locus_tag="ACZPD4_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1377526.1"
                     /translation="MWYWIIVALLVSGCAAMLFVSFSGIKQSKAELARAEGRGRKKRR
                     DEYEDEYEAEVRTRSKREREAQAYAKDQRPKRKKRRWKIILEDIDSWEKYSFTFCDSV
                     GIGRGKDGSMYERYLPLTSDGRISKLHCVIIHKNDKLYVKDEDSRNGTYLNGVRIDRP
                     MEIQRDDIIGVGETRLEVQRILRESD"
     gene            4795..6030
                     /locus_tag="ACZPD4_00180"
     CDS             4795..6030
                     /locus_tag="ACZPD4_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR1377527.1"
                     /translation="MSIIRPFKGIRPRADLASAIAALPYDVYNSEEAREIVAAEPKSF
                     LQIDRAETLLPKGTNPYSEAVYKTASETLNRMIDEGSFLQDKEPCYYLYALTMNGRTQ
                     TGLVGCASIDDYCNNVILKHENTLEAKEQDRIRHIDACSAQTGPIFLAYRADKKLGEI
                     IKNVRLTPEVYDFTSDDGIRHEVWRIDKMSTIVDISKIFEAIPHLYIADGHHRAASAV
                     KVGLKRRAEDLHFSGTEEYNYFLSVLFPADELAIYDYNRVVTDMNGYTFEQFLDTIKS
                     RFDVALAGVEAVRPQKKGEFGMYADGNWYRVNAKADLFSGDPVKDLDVSVLQDHILSP
                     VLGISDPRTDSRIRFIGGIRGLDALKDAADQTGGIAFAMYPTSMTELMGVADAGLLMP
                     PKSTWFEPKLRSGLFIHRF"
     gene            6068..6931
                     /locus_tag="ACZPD4_00185"
     CDS             6068..6931
                     /locus_tag="ACZPD4_00185"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIR1377528.1"
                     /translation="MERNELCWCGSGKKYKKCHMPIEEKILLHSERGEIVPTRAILKT
                     AEQIEKIKESARLNTAVLDEVAKHIRIGMSTAEIDDIVYTFTKEHGGIPAPLNYQGFP
                     KSVCTSLNNEICHGIPDKNIILQDGDIINVDVSTILNGYFSDASRMFKLGTVSERAEK
                     IVRVTEECVNLGLAAAKPWGHLGDIAHAINTHARENGYSVVEEIGGHGIGLEFHEDPF
                     VSYVTPKGSEMLLVPGMIFTIEPMINEGSPDFFVDEDNGWTVYTIDDGLSSQIEYMVL
                     VTNDGVEVLTK"
     gene            complement(6980..7867)
                     /locus_tag="ACZPD4_00190"
     CDS             complement(6980..7867)
                     /locus_tag="ACZPD4_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1377529.1"
                     /translation="MREKRKSRKRHNKRGQILTPVIFLTSLLLFAAGVGIFIYPALSN
                     YLAQREQKEVIEEYAQTVEQIDKDKMARQWELAEEYNETLLGDPVHDPFIPGTGYALP
                     DNYESVLNVNKDGVMGYLKIPKIKVDLPIYHGTSEEVLEKGAGHVDVTALPIGGVNRH
                     PVISAHRGLPSAELFTRLDELEKGDRFFLHILDKTLAYKVDQVRVIKPEELEQLQTYH
                     DKDYVTLLTCTPYGVNTHRLLVRGERIPYEVAEENGGEAFDDEKNQGMPQWVKEYVVM
                     IIAGILLLVFAGRMFAARK"
     gene            complement(7974..9581)
                     /locus_tag="ACZPD4_00195"
     CDS             complement(7974..9581)
                     /locus_tag="ACZPD4_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIR1377530.1"
                     /translation="MDKKTKMKEKKASGIWKKVLGICLAVIMVIGMTMPAWAQIDAES
                     KGSIKVTADESGWTASAYRVIDVNFDFDNQQPKEPAYVWNKNAAEWVRTNFKDYIEAD
                     GNGVTEAFITMDTDKDAVKVKEFADKMSSAIAADTIQMGAAGSVQPGEGQTEATIGNL
                     SMGGYLLLMTGGSKIYSPVFTHIYPKFSDDTKEWAIVDGDITITAKGTNPTIKKTVKN
                     ADGKEISTTAIGDTVTYELTVDIPVYPDSANVKTFVFGDVLPSGVALVEDSVAINPSG
                     AQTDLSEHFIKTEGKFEYSVKDYGNLKTVVNGASQIIVTYKAKVVSEAYETPDNLKNA
                     AYLKYNSASYNTQGVEKKINVDRQLYTYGVKIKKVDNKDENTALQGAEFALKKGDTEI
                     KFAKSGKMYYPAADGDAKLTTDGNGEMLIAGLDLGTYTLTETKAPSGYTLPRNPETTI
                     TLSEKDAVNGTLDESKAEGTNVKANSASVDTQKTWQLNFVLTNGKSNFNLPITGGIGT
                     AVFTIGGIMIMAGAVMLLTYAIRRSRR"
     gene            complement(9599..10624)
                     /locus_tag="ACZPD4_00200"
     CDS             complement(9599..10624)
                     /locus_tag="ACZPD4_00200"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR1377531.1"
                     /translation="MWQNEISISDPNYDSSSGRYHRGDTSYTVYTLKGDPAEKNVYYV
                     PLTNEEIAAYKKGMEDPESAEGMDAQNREKVRAAACFENLAAGEYVLKETTPPNGYGA
                     SDPIRFKLSIQEGEENQFVLPYGVSLESPLSNKENVTLQVRNEKNSGVVSFTKENRKG
                     EPLKNAKFALYRLICSDPSHGGKHDKDLIRHDENGSLDAGYAYKDCWKLQGTAVSDEN
                     GTVEFLNLPAEEGTEYRLAELKAPSGYVTAKGQWKVTYEKETHELKITGSVKRPPAFS
                     GEGTDENPYKVINYTYAEFPLSGGRGYSYLAGTVLIAAAAGLWFWYDSRKRKTVRSRG
                     MRKRGYK"
     gene            complement(10643..16120)
                     /locus_tag="ACZPD4_00205"
     CDS             complement(10643..16120)
                     /locus_tag="ACZPD4_00205"
                     /inference="COORDINATES: protein motif:HMM:NF039610.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HIR1377532.1"
                     /translation="MGRKNRIFKGNLKASQILAVLMLIVMIIRPIMEVSAKPGEITTE
                     NPSAEVGKEGESLSDGIDGSALYPLEENGQNGQTSENDAVRQNGQTSDETDAKNSPNA
                     SDRTVSEGSSEEENMTSAQQESLCNDEYCSHIYYDLDGRQHALCALGERMTETDVYRT
                     VEDSAVYEAMYFASIPNLYEASAFGGATTFEKLHVNTVYALFYKEDPIEPGNAGKKEI
                     PKTYRMISVDEAGLFGTVIPEGCSYVAFEMEVTNKNGEAREIDSQIVYNLRQAANPND
                     PYHEIKKEPVIDAAGEVKQRGSGSFVFTQKTRDCFYWDMSYHTSYWGGHPSSSNNILD
                     ELAIYVDNDKDGNYDTKTDKVWDELWVSYADKNGKIVSKKVTRETRYELCTYYAFGKG
                     SGLTELSLLAISNGELNEKGGFKHPSDNKEDDKVYYQLYNIASGDNFFILRNLDDSHR
                     IWRSYETDGDRYVAFKDQMYGTSGALVASGVEGGKTEAADTVEYRLCENPEDGQAVET
                     EWKKMDQALPESSGTGQASEDMENEVFYKTFFTHEKLSDKEKYIQFRICENRADGKVV
                     KYKTDITEIDDTYAFPCFFADVLQKKSLSYTSEQSKINGTWSSIFKASDHGDEGKNIP
                     IAKNKSYDPKYYYANTTFYDYYSDYELQGKRLSEVNGSPYEYETSFGILNDGISKYFQ
                     GKNEKAAAWYYGKYIRHPAVTDGDNPLNNSSPGNMSGNQIGPNDWHSGNTLKYAYGHV
                     DKQLEDGKISTAGVKLPQFDESFLRGNNAFHSALGYVYNNVSFPFFLADDGYWEYSSL
                     KSDHVLRLKEDPDKGYYLDVGEKEDALKTGDRDVAMGKDQFGFFPFNDKNDATKENSF
                     YRLDRMKTNFCFGMKMELEFKLTEDGKVRMPGEKAGEYIEKPIEFEFSGDDDFLLYID
                     GKLVLDMAGKHNPLEGHINFETGAVDVYGLNKDGATEDGYPINKNQHHAQANIYEKND
                     ANGPCLDMEELSQGIHKITIFYAERGAGVSNLKIRFNFPKTNQLDLTNQVSTASANQD
                     VFGKALGYIGSFTYKILNQATSGRQIPVEDSVGYLRNEGAVKFNDFKNSGTYRGSKTG
                     ESELKEADPDAETDGPIIGERSDVIKLVNKDSGKNLDQYQMSEEIASGKYWTTIDCAE
                     NGGSVDLSKAAYLQMSVYNNSDASSATGWSLKARLTDRHGNVITRPASQMTYYGYSNS
                     MPKGQWSIVRLNLADWNSGISDFDMKNVAKVQFAFARDRGSLYMADMDFKGKIIPNQS
                     TGFYASDDQISDYESLGTKERPLSYNEAVLNPVSGAFFRLNPIQETDVVNNGNDLMVR
                     SRGLLELTDGNKASFVDKFREGSYLQITQDDPLRNKVFRTSWSILENAVYDGASGMYT
                     EGYIADNILALHPTARTVENDPEILNNTVTDVRTYTASDDRNVKSADGKGYHYPSSNR
                     EGHVSEKNETFVFRSYLDPDNLQKNGIHLTVAYKNDLISGGLIITKTVDQIDPKKDHT
                     YKFHVHYTDVAGMSLEGNLGSGSMSESGSSIVQEIDVTVPAGKQTASVAVYGIPKETV
                     YTIHEENEIPQNIEIGGNYTYPQNVAKNFVQTDPDRGGVRTGDEYDHKVKVGTKKYNV
                     NGTEVEYKTAEGTMKESVQEVTFANKQVPAHGEIKITKKLQGKAYGEERGYLFHIHYK
                     TTDENNKEKVVVVPQTVVVKAGELTGSTVYENIPLGAQYEVHEVDETALLTLEADAGS
                     TVLGSDDPLSDTEQNHSHTDVKAEKKHISIIPEEEGAIGSECAKKGFWTAQGGTGESR
                     HNFTFTNIGSHNVAVLKTDSSRNVPLENAGFTLYDTEDNIV"
     gene            complement(16292..17224)
                     /locus_tag="ACZPD4_00210"
     CDS             complement(16292..17224)
                     /locus_tag="ACZPD4_00210"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIR1377533.1"
                     /translation="MAGKVAVLNDLAGFGRCSLTAAISVLSAMGVQPCPLPTAVLSAQ
                     TGFESYFFQDLTEQMGMITEEWKKSGAQFDGIVTGFMSDHRQIGEVNHFLDLFHKEGT
                     FLLVDPVLGDNGKRFSVFDGLFQKEMKQLVKRADIVTPNVTELCLLTDYDYDKIQNIS
                     ETGDREALFSEVSKAAGRLLDSGVSEVIVTGVLFEEQDAAKECTVGKETGTGGRKVAN
                     LTVTREKTTAGISSFIGASYSGTGDLFASVIAGGKARGDRTEDSVRLAGEMIEKAVRE
                     SAALGISGKEGAEYEKYLWMLCKKTKESGEKKGK"
     gene            complement(17209..19275)
                     /locus_tag="ACZPD4_00215"
     CDS             complement(17209..19275)
                     /locus_tag="ACZPD4_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1377534.1"
                     /translation="MNREALFCDGTQDYVIPAEPKENEKIKIRFRTAHNDVEEVHILT
                     GNSSYPMWKIVSEGEFDFYEIEWQLSDVPFSYLFEVKSGDQICYFSRCGVSDQREDFY
                     AFMIVPGFSTPEWAKGAVMYQIFVDRFCNGDPTNDVEDGEYIYIGAPSVKIKDWSKVP
                     AAMDIRNFYGGDLQGVMDKLDYLQDLGVEVVYFNPLFVSPSNHKYDIQDYDYIDPHYG
                     KIVSDGGETLPKGAKDNTGATKYQKRTGDIRNLEASNELFARLVEEMHKRGMRVILDG
                     VFNHCGSFNKWIDRERIYEQQPEYPKGAYVSGESPYRKFFRFHDERKEAWPYNKSYDG
                     WWGHDTLPKLSYEDSPELEQYIMDIGKKWVSPPYNADGWRLDVAADLGCSNEYNHMFW
                     KRFRKEVKEANPEALILAEHYGDPGEWLQGDEWDSVMNYDAFMEPLTWFLTGMEKHSD
                     ERRTDLWGNADNFIGGMRHFMASMLTPSLQVAMNELSNHDHSRFLTRTNHIVGRAEHV
                     GAKAAEEGVNYAVMREAVTVQMTWVGAPTVYYGDEAGVCGFTDPDNRRTYPWGRENKE
                     LLAFHKEMIRIHKQEKPLRKGSIKLLYAERNVLAYARFQEDEQIIVILNNSKDLKELT
                     VPVWFAGVPKQGRMERLMYTYEEGYTTEYDEFIVENGEIVINMGRHSAIVMKPISEGE
                     KTWQGK"
     gene            complement(19279..21120)
                     /gene="ftsH"
                     /locus_tag="ACZPD4_00220"
     CDS             complement(19279..21120)
                     /gene="ftsH"
                     /locus_tag="ACZPD4_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1377535.1"
                     /translation="MDNNRNYRSVNGFTIIIFMVIVFGALWMANRMQMHRQEMTYTEF
                     VKQVEAENVTEVYIDQNKAVPTGTVVFALKDTDENRSVNVSNVEKVEKLLDKNEVLYQ
                     VSAIPETSMLSSVLLPTVITLGGIMLLFFLMNRQGNGANAKAMNFGKSRARMTNHDEI
                     KVTFANVAGLQEEKEELAEIVDFLKAPKKYVQVGARIPKGVLLEGPPGTGKTLLAKAV
                     AGEAGVPFFSISGSDFVEMFVGVGASRVRDLFQDAKKNAPCIIFIDEIDAVARRRGSG
                     LGGGHDEREQTLNQLLVEMDGFGVNEGIIVMAATNRKDILDPAILRPGRFDRNVLVGR
                     PDVGGREEILKVHAKNKPLAEDVDLKQIAQTTAGFTGADLENLLNEAAIIAAKDNRMF
                     IQQKDIRHAFVKVGIGAEKKSRIVSEKERKITAYHEAGHAILFHVLPDVGPVYSVSII
                     PTGGAGGYTMPLPEKDEMFNTKGQMLQEITVSLGGRVAEEEIFDDITTGASQDIKQAT
                     AIAKSMITKFGMSERLGLINYDNDSDEVFIGRDFGHTSRGYGEKIAGTIDEEVKRIID
                     ECYAKARSILEEYHDVLEACAKLLLEKEKITRSEFEALFEDGVSEEG"
     gene            complement(21136..21663)
                     /gene="hpt"
                     /locus_tag="ACZPD4_00225"
     CDS             complement(21136..21663)
                     /gene="hpt"
                     /locus_tag="ACZPD4_00225"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1377536.1"
                     /translation="MAETIREMISEEKVDERIRELGRQISEDYAGKQIHLICVLKGGV
                     FFMCELAKRITVPVSMDFMCVGSYGDGTASSGVVRIAKDLDESIENKEVLIVEDIIDS
                     GNTLYYLIDVLKKRNPASMRLCTLLDKPDRRVKDVKVDYCGFEIPDEFVVGYGLDYAQ
                     KYRNLPYIGVVEGVE"
     gene            complement(21620..23158)
                     /gene="tilS"
                     /locus_tag="ACZPD4_00230"
     CDS             complement(21620..23158)
                     /gene="tilS"
                     /locus_tag="ACZPD4_00230"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1377537.1"
                     /translation="MRIVKSCVLRGASYIKTRIWYSILMMEKAGRETEKLIEELIVQF
                     HMIEHGDVVIAGVSGGADSVCLLLTLCSLREKFGFEVKVCHVNHCLRGAAADADEEYV
                     KKLCGELGVECRVFRKEVELIAEKRKQSCEEAGREVRREAFEQMCAENGGTKIATAHH
                     RDDQAETVLLNIARGTGIRGLCGIVPVRDNRIRPLLSLTRDRIESYLRERGIVWRVDA
                     TNEEDDYTRNRIRHKVLPVLENEVNDQTVKHLESLSRQAEEICEYLDYSVRQLWKTCV
                     SVRKKDQEQKESPIELVIEKESFFQSFPAVRKLLLQKCIGKVRGGQKDLTSAHLQSVS
                     ELFDRQVGKQIDLPGGVAAKRTYDGIEIIRQTQSRAETGKAAPESDAAGKLELRFVEN
                     GDILQAEEIPQKSYTKWIDYDIIKCGLCVRTRQSGDYLVIDDQGRRQKLKAWFINEKI
                     PKEERDRMLLVADGSHIVWIPGYRMSRAYKVTEKTKNIVEIKITEEEKDGRNDQRDDF
                     GRES"
     gene            complement(23160..>24542)
                     /locus_tag="ACZPD4_00235"
     CDS             complement(23160..>24542)
                     /locus_tag="ACZPD4_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1377538.1"
                     /translation="YASSLKQLSHTFLELEAHKEEFSGADIDGMFDRVKERVCKKCEK
                     NAWCWGENFVHTYQMGYDVLSAVDHYGNELNVETKRKLMQRCEKAPRFLREMLEGFRD
                     ARQTIIWTNRLARSREGCAFQMDLFADIMKQTVRELEKSIFTDERMEKRIAGALRKCG
                     MRVLYTYLFLNGEGKFEVHVTVRSTGKEKGTAKEVVQAVSEAAGRRFVLAHGSPQVVG
                     FRYMTIVCREGPSYYTLSGTAKIGKGCAKISGDNFTLMELPGGKKAAALSDGMGAGET
                     ACKESTLVIELLEELLAAGFPEKAAVQMINTTLATGREEIHYSTVDLSVFDLYSGECE
                     IVKAGASSTFIKKKDCVEHLSSTSLPIGVVHHIEIDSVRRKLEDGDFVIMVTDGILDA
                     LPVGEQDILLETIIQGSAIVNPKEMAHHILEQVLAWTGREPADDMTVIVVGMWEICDR
                     NTLQARMDSV"
BASE COUNT         5887 a         6759 c         4364 g         7532 t
ORIGIN      
        1 atgtctctta cttatagtta ctccgatcct tatttcaatg atattctggg agggattcaa
       61 tcttactgcg catctcacaa tgcgtttatt tccctgatcg taagctattc ccagctgcat
      121 aatatgactc ccggactcaa aaagcagctt tcggaactgg acggactgat cattacggaa
      181 attccgcccg aaactcttga atttattaca acgctcaata aaaaaatcgt atttgtcgac
      241 aactatattg acggatactg caatatagga tacaacaccg tatatgcaaa ccaactgatc
      301 ttcgaccaca ttatcaaatg cggttacaaa aagatcgcct acatcggcgg cgcttccaac
      361 tcatccgccc catccgactt cgactcctgc tgccgcatga tgatcttccg tgaaacgctt
      421 cgggcaaata atattcctta cgatcccgaa ctgatctata actgcgactg ggattcaaaa
      481 gtatgtgcgg cacaggtaaa ggaactgctc tcaaagcatc ccgaaaccga agtgatcttc
      541 gccggttcgg attctctggc aagcgtcatc ctttctcagc ttaaaaagct gaacttaaaa
      601 tgtcctcagg acatcggtgt cgtcggcttt aatgacatct ccctttccaa gaatttttcg
      661 cctgccctga caaccgtccg gcttccttct gccgaaatgg gaaaactggc ggcagaagtg
      721 ttgattcgtc agatcaaaag caactccgta ttaaaacagc agatccttct ccctgtagaa
      781 ttaaaagtac gggagtccac tcgaaaaatc cgataaaaac tatccaaaac gccaaaagtg
      841 cagtttcctt tccacttccc ggaaaccgca ctttttcttt ttatatcttc catcgccgaa
      901 agagccaaaa ggcagaaccg tttctcggtt ctgccttttt ttaaccaact ttcaatttaa
      961 acttctgaat ctctttttca ctggatacac gctcgatcat agcgccgagc cctctaagtt
     1021 tctcttcaaa ccgctcatat cctctctgaa tataaacgat atcatccacg atcgtaatgc
     1081 cttccgccgc aagacccgca atgcaaagcg ctgctcccgc gcgaagatcc ggcgcgctca
     1141 cacgagcagc cgaaaaccgc tcaacgcctt caatcgtcgc agaattgcct tccactttta
     1201 cctgtgctcc catacgggcc agttcaccga gatatttgaa acgattctca aaaatacttt
     1261 ccgtgatcgt actcgttcct tttgccaaag cgagcgccac cccgatctga ggctgcatat
     1321 ccgtcggata tcccggatac ggaagcgtct tcacctgggt acacctcaga cgcttctttg
     1381 caacaacgcg aaccgcatcg tcaaactctt ccacttcgca tccgatatcc actaatttag
     1441 agatcgtcgc atcaagatgc ttcggaatta cgttcaatac cgttacgtct ccttttgtcg
     1501 ccgcagccgc aaacataaat gttcccgctt cgatctgatc cggtatgatg gaatactccg
     1561 tcttatgaag cgatctcacg ccgcgaatct tgatcacgtc agtacctgcg cctctgatat
     1621 tcgcgcccat gctgtttaaa aagtttgcca catcaacaac atgcggctct ttcgccacgt
     1681 tttccatgat cgtaagacct tcggacatag cagccgccat catcacattg atcgttgccc
     1741 cgactgtcac aacatcaaaa tacaaatgcg ttccgcgaag tttctccgcc tctgcgacaa
     1801 tcttgccgtg ttcaatatca acgtccgctc caagagcccg aaatcctttc aaatgctgat
     1861 cgatcggtct gcttccgata ttgcatcctc ccggcagcgc gacttccgcc cttttatatt
     1921 tcccgagaag agctcccagc aaatagtaag acgctctgat cttcttaatg taatcatact
     1981 ctatattaaa atcgccgatc gtacttccgt tgatctttac tgtatgacga tcgatcctct
     2041 gcaccatcgc gccgattccc tcgatcgctt ccaacatcac attcacatca ttcacatcag
     2101 gaagattatc gatctgaaca gtctcatccg tcatgattgc agccgccaaa attgcaagcg
     2161 ccgcattttt cgcgccgccg atctccactt ctccgacaag cggatttccg ccttttatga
     2221 tatattgttc catatcacac ctctgtttag attttattat catacttgta ctttcccgta
     2281 cgttttttct atatctcttt tctatcttat attctttata atatcttttc agtctgccgt
     2341 ttttattcta ccacatatcc ggcttttttt aaatcccaac ttttgtcaac gtatcattct
     2401 agtgttgttc actgtacatt gtcaagtttt ttacttcttc caggacattt tctatcgtat
     2461 cttcgatctc gcatccgtct tcacagatcg taatatccgc atatttttca aagaacttca
     2521 cgcgctcttc ataaagctgc cgaaacgtct gtccgtcgcg cagaacaact ccgcgctttt
     2581 tcggattgcg gattctcttt tttattgtct gataagacgc tttcaaatat acgaccgttc
     2641 cgatattttt ataatgtttc atcgcctctt cgcaataaat cacacttccg cccggggaaa
     2701 tgaccgcgtt ttccgcatcg acgtctgcat tcaccctgtt ttcgatctca ataaacccat
     2761 cttctcccac ttcggctata atctcacaca aaagtttctt ctcctgttcc tggatcaaca
     2821 gatctgtatc gataaactgc attccgagac gcttcgccac aacgatgccg accgtactct
     2881 ttcctactgc gggcatccct ataaaaatca aatttttttt catatgtatt ctccgcctta
     2941 ttttattccc cacctaatat acattactat atcttattta ttttatcaca tttcatctta
     3001 atagtaaatc aagagagctt ctttgaaaat ttctgtaaaa tatacataaa attcaagcaa
     3061 atattccatg taattttcaa ccaaaacata ttttttgcca aacagttccg cttcgcctca
     3121 caaaacttgc ataaaacagt tccgcttatt taaaaaatct gcgacaacat cttctgtggt
     3181 cacaacggcg tttgtataac tcctgaaaca gcattacctc cacaagatcc ctcagattct
     3241 ccatagttat tgcttcatct tcggataaat cttcctcctt ctcatcctct tcccggcaaa
     3301 tatgttcatg ccgtctgata ttgtcacaca cccgcgtgca catcagcttc aactgcagtt
     3361 tatcaggata tgcgtcgtac accatactgt tttcgtattc catacggtcg cattcttctt
     3421 ctacatacgg aagaatccgt ttcggaatct tcggatacat acttttcaaa tattcaagat
     3481 ccattctctc aatccgctcg tcatcaaaag aaaagggcat cggataagcc atataataag
     3541 gcagtttttc gttcatcgca ctcactcccg aactgatatt atatgtgtac tatatgttca
     3601 ttccccgaaa atgtgactgc caaaaatgcg ccgcttccta tacggcaaac agcccgcttt
     3661 acagtgcggc aactgtctgc cttcccgcaa aacggactgt tcttttctat atgctgtgtt
     3721 taatcgctct cacgcaaaat cctctgcact tcaagccgcg tctccccgac tccgataata
     3781 tcgtctcttt ggatctccat cggccggtcg atacggacac cgtttaaata tgtaccgttt
     3841 ctcgaatcct cgtcttttac atataatttg tcattcttat gtatgattac acaatgaagc
     3901 ttggagatcc ttccgtcaga agtgagcgga agatatctct catacatgct tccgtccttt
     3961 cctctgccga tccctacgct gtcgcaaaat gtaaagctat atttttccca actgtcaata
     4021 tcttccagaa tgatcttcca gcggcgtttc ttacgtttcg ggcgctgatc ctttgcatac
     4081 gcttgcgcct ctcgttctcg ttttgaacgc gtgcggactt ccgcctcata ctcatcttca
     4141 tattcatccc gacgtttttt tcttcctctt ccttctgccc gcgccaactc cgctttgctc
     4201 tgtttgattc ccgaaaacga tacaaacaac atcgcggcac agcccgacac aagtaaagct
     4261 actataatcc aataccacat aatttcctct cctttaaaaa ttaatactac tatattttta
     4321 taattcgggc aagtaaaatg tcggtttttg tctaacagtt cagccataca gatatccaag
     4381 aagcacaaaa catgcttgca tgtttttgca agtggatatc tgtatgtgct aagcgcctat
     4441 tcatgagtaa aacagtggcg ccagccacaa taagcacgca gtgcggtttt acgaatggcg
     4501 cgggctgaac tgttacgata gttcatccat acagtatcca agaagcacaa aacatgcttg
     4561 catgtttttg caagtggata tctgtatgtg ctaagcgcct attcatgagt aaaacagtgg
     4621 cgccagccac aataagcacg cagtgcggtt ttacgaatgg cgcgggctga actgttacga
     4681 tagttcatcc atacagatat ccacttttac cgcacttcag gtcgactctc ccgtgacaaa
     4741 ccgctttcgc ctgtggtata gttatctgac agatgatttt taggaggtac tactatgagc
     4801 attatccgac catttaaagg aatccgccct cgcgcagact tagcttctgc catagccgcc
     4861 cttccttatg atgtatacaa cagcgaggaa gcaagagaaa tcgtggctgc cgaaccgaag
     4921 tcttttcttc agatcgaccg agccgagacg cttcttccaa aaggcacaaa tccatacagc
     4981 gaagcagtat ataaaacagc atctgaaaca ctgaaccgta tgatcgatga aggttctttt
     5041 cttcaggaca aagaaccttg ttactactta tacgcgctta caatgaacgg aagaacacag
     5101 acagggcttg taggctgcgc ttcgatcgac gattattgca acaatgtgat cttaaaacac
     5161 gaaaatacac tggaagcaaa agagcaggac agaatccgtc acatcgacgc atgcagcgcc
     5221 cagaccggac cgatctttct cgcttaccgc gccgacaaaa aactaggaga gatcatcaaa
     5281 aacgtccgat taacacctga agtctacgac ttcacctctg acgacgggat aagacacgag
     5341 gtgtggcgta tagataaaat gtcaacgatt gttgacattt ctaagatttt tgaggcgatt
     5401 ccacatctgt acattgcaga cggacatcac cgcgcagctt ctgctgtcaa agtcggactg
     5461 aaacgcagag cagaagatct ccatttttca ggaacagaag aatacaatta ttttctttct
     5521 gtcctcttcc ccgcggacga actggcgatc tacgactaca atcgagttgt tacggatatg
     5581 aacgggtata catttgaaca atttcttgat acaataaaga gcaggttcga tgtggctttg
     5641 gccggcgtag aagcagtccg cccgcagaaa aaaggcgaat tcggaatgta tgccgacgga
     5701 aactggtatc gtgtaaacgc aaaagcagac cttttctccg gcgatccggt aaaagatctg
     5761 gatgtttccg ttttacagga tcacatcctt tccccggttc tgggcatcag cgatcccaga
     5821 acagattccc gcatccgttt tatcggcgga atccggggac ttgacgcgct gaaagacgcc
     5881 gcagatcaga caggcgggat cgcatttgca atgtatccca cttctatgac tgaattgatg
     5941 ggcgttgcgg acgccgggct tctcatgcct ccgaaatcaa catggtttga accaaaactg
     6001 cgaagcggcc ttttcattca ccgcttttaa aatacagtaa taaaataact acaaggagat
     6061 gttattcatg gaaagaaatg aactctgttg gtgcggcagc ggcaaaaaat acaaaaaatg
     6121 ccatatgccg atcgaagaaa aaattcttct gcattcagaa cgcggcgaga ttgttccgac
     6181 acgggcaatc ctcaaaacgg ctgaacagat cgaaaagatc aaagaaagcg ccagattaaa
     6241 taccgctgta ctcgatgaag tcgcaaagca catccgcatc ggaatgagca ctgccgagat
     6301 cgacgacatc gtatatacgt tcacgaaaga acacggggga atccccgcgc cgcttaatta
     6361 tcagggattt ccgaaaagtg tctgcacttc gctcaacaac gaaatatgcc acggtatccc
     6421 ggataaaaat atcattcttc aggacggcga tataatcaac gttgacgtct ctacaatctt
     6481 aaacggctac ttttccgacg cctcccgtat gtttaagctc ggaacggttt ccgaaagagc
     6541 cgaaaaaatc gtccgcgtaa cagaggaatg cgtcaacctc ggacttgcgg ccgcaaaacc
     6601 gtggggacat ctcggtgata tcgcgcatgc gatcaacact catgcccggg aaaacggcta
     6661 ttctgtcgta gaagagatcg gcggacacgg aatcggactt gaattccacg aagatccgtt
     6721 tgtcagctat gtcactccaa aaggaagtga aatgcttctc gttcccggta tgatcttcac
     6781 gatcgaaccg atgatcaatg aaggcagtcc cgacttcttc gttgatgagg ataacggttg
     6841 gactgtatac acgatcgacg acggactgtc atcccagatc gaatacatgg tacttgtcac
     6901 aaatgacggc gtagaagttt tgacgaaata gtaaataccg cagaacagta gtcttctttt
     6961 cttctgttct gcggttcttt tattttctcg ccgcaaacat cctgcctgcg aaaacaagca
     7021 gcaagatccc tgcgataatc ataacaacat actctttcac ccattgcggc attccctgat
     7081 tcttttcatc atcgaacgcc tcacccccat tttcttccgc cacttcatac ggaatccgtt
     7141 ctccgcgtac taaaagcctg tgtgtattca ctccgtaagg cgtacaagtc agaagcgtca
     7201 cataatcttt atcgtgatat gtctgcagtt gttccaattc ttccggctta ataactctca
     7261 cctgatctac cttgtaggcg agcgttttat caaggatatg gagaaaaaaa cgatctccct
     7321 tttccaattc atctaacctt gtaaataatt ccgcgcttgg aagacctctg tgcgcgctga
     7381 tgaccggatg ccggttcact ccgccgatcg gaagagcggt cacgtcgaca tgtcccgcgc
     7441 ctttttccaa aacttcttca cttgtgccat gatagatcgg aagatcgact ttgatcttcg
     7501 ggatcttcag ataccccatt acgccgtctt tgtttacatt cagcacactt tcataattat
     7561 cgggaagcgc atatcctgtt cccggaataa acggatcatg aacgggatca ccgagaagag
     7621 tctcgttgta ttcttctgcc agttcccact gtcttgccat cttgtcttta tcaatttgtt
     7681 ctacagtctg agcgtactct tctatgactt ctttttgctc acgctgcgcc agatagttgc
     7741 ttaacgccgg ataaatgaaa atcccgacgc ccgccgcaaa taaaagcagg gacgtcagga
     7801 atatgaccgg tgttaatatc tgcccgcgtt tattgtgccg ttttcggctt tttctttttt
     7861 cgcgcataca gacaaaccct tttctttctg tctttttctt tctctatttg tttttctgcc
     7921 ttttctctgt ctgttttccg ttcggaactt ttttcttttc ttcctgaaac ttcctatctt
     7981 ctgcttctgc ggatcgcata tgtaagaagc attaccgctc ctgccatgat cataattccg
     8041 ccgatcgtaa agactgccgt tccgatgcct cctgtaatcg gaaggttgaa attacttttg
     8101 ccgtttgtaa gaacgaagtt caactgccat gttttttgtg tatcaacaga tgccgagttt
     8161 gctttcacat tcgttccttc tgcttttgac tcatccagcg taccgtttac agcgtctttt
     8221 tcagacagcg tgatcgtcgt ctcaggattt ctcggcaaag tataacctga cggcgctttc
     8281 gtctctgtca atgtatatgt tccaagatca agacctgcga taagcatttc tccatttcca
     8341 tctgtcgtca gttttgcatc tccgtctgct gccggataat acatctttcc gcttttcgca
     8401 aactttattt ctgtatcgcc ttttttcaaa gcaaactccg caccttgaag cgccgtattc
     8461 tcgtccttgt tgtctacctt tttaatcttt acgccatacg tatataactg acggtccaca
     8521 ttgatctttt tttcaacgcc ctgtgtatta taagaagcgc tgttgtactt caaatacgct
     8581 gcgtttttca agttatccgg tgtttcataa gcttctgata caacttttgc cttataagtt
     8641 acgataatct gacttgcacc gttgactacc gttttcaaat tgccgtaatc tttgacgctg
     8701 tattcaaatt ttccttctgt ttttataaag tgttccgaaa gatctgtctg cgcacctgac
     8761 gggttgattg ccactgaatc ttcaaccaac gctacaccgg atggaagaac atctccgaaa
     8821 acaaatgttt ttacatttgc actatcagga taaaccggaa tatcaactgt cagttcgtaa
     8881 gttaccgtat ctccgatcgc cgtcgtagat atttcttttc cgtctgcatt ttttacagtt
     8941 ttcttgatcg tcgggtttgt tccctttgcg gtaattgtaa tatctccgtc tacgattgcc
     9001 cattcttttg tatcgtcgct aaacttcgga taaatatgtg taaataccgg agaatagatc
     9061 ttgcttccgc ctgtcatcag cagaagatat ccgcccattg aaagatttcc gatcgtcgct
     9121 tccgtctgac cttcacccgg ctgaacgctt cctgctgctc ccatctgaat cgtatctgcc
     9181 gcgatcgccg acgacatctt atctgcaaat tcttttacct taaccgcatc cttatccgta
     9241 tccattgtta taaacgcttc tgttacgccg ttgccgtccg cttcaatata atctttaaag
     9301 tttgttctga cccattctgc ggcgtttttg ttccacacat aagcaggctc tttcggctgc
     9361 tggttgtcaa aatcaaaatt cacatcgatc acccggtacg ccgatgctgt ccatccgctt
     9421 tcatctgccg taactttgat agaacctttc gactctgcat cgatctgcgc ccatgccggc
     9481 atcgtcatcc cgatgaccat gattaccgca agacagattc ccaacacttt tttccaaatg
     9541 ccgcttgcct ttttttcttt catttttgtt ttcttgtcca ttaacttttt cctccttatt
     9601 atttatatcc cctttttctc atgccgcgtg atcttacagt ttttctcttt ctcgaatcat
     9661 accaaaacca caacccggct gccgccgcga tcagaaccgt gcctgcaaga tagctgtatc
     9721 cccttccgcc tgataacgga aattcggcat aagtatagtt gatcacttta tacggattct
     9781 catccgttcc ttcccccgaa aatgccggag gcctttttac gcttccggtt atcttcagtt
     9841 catgagtctc tttctcatat gtgactttcc actgaccttt cgccgtcacg tatccgctcg
     9901 gagctttcaa ctccgccaga cggtactctg tgccttcttc tgccggaaga tttaggaatt
     9961 cgactgttcc gttttcatcc gaaaccgccg ttccctgcaa tttccaacaa tctttatacg
    10021 catatccggc gtcgagacta ccgttttcat cgtgccggat cagatcttta tcatgctttc
    10081 ctccatgaga cggatcgctg cagatcagcc ggtacaaagc gaacttcgcg ttcttcaaag
    10141 gctctccttt tcggttttcc tttgtaaagc ttacgactcc gctgtttttt tcatttctca
    10201 cctgaagagt cacattttct ttattggaga gaggactttc caaacttact ccgtaaggaa
    10261 gtacaaactg attttcctct ccctcctgta tcgataattt aaaccgaatc ggatctgacg
    10321 cgccgtatcc gttcggaggc gtcgtctctt ttaaaacgta ttctcctgcc gcaaggttct
    10381 cgaaacatgc cgccgcgcga actttttcac gattctgtgc atccattcct tccgcgcttt
    10441 caggatcttc cattcccttt ttatatgcgg caatctcttc gttcgtcagc ggtacataat
    10501 aaacgttttt ctcagccgga tcgcctttca gcgtatatac cgtataagac gtatctcccc
    10561 gatgatacct tccgctcgaa ctgtcataat tcggatcgct tatactgatc tcattttgcc
    10621 acatataaac ggccgtccgg ttttaaacga tattatcttc cgtatcataa agcgtaaaac
    10681 ctgcgttttc caaaggcacg tttcgggaac tgtctgtttt caagaccgca acattatgag
    10741 agccgatatt tgtaaacgta aaattatgac ggctttctcc cgttccgccc tgcgctgtcc
    10801 aaaacccttt cttcgcacat tcgcttccga tcgccccttc ttcttccgga atgatcgaaa
    10861 tatgcttctt ctctgctttt acatctgtat gagaatgatt ctgttcggta tccgaaagtg
    10921 ggtcatcact tcccaatacc gttgatcctg cgtccgcttc caaagtcaga agagcggttt
    10981 catctacttc atgtacttca tattgtgcgc caagcgggat attttcatag acagtacttc
    11041 ctgtcaattc ccctgctttt acaactacag tctgcggaac gacaacgacc ttttccttat
    11101 tgttttcatc cgttgtttta taatggatat ggaacaaata accccgctct tctccatacg
    11161 cttttccctg aagttttttc gtaatcttaa tttctccgtg agcaggcact tgtttattcg
    11221 caaacgtaac ttcctgtacg ctttctttca ttgtaccttc cgccgtttta tattcgactt
    11281 ccgttccgtt cacattatat ttctttgttc ccaccttcac cttgtggtca tactcatcac
    11341 ctgtgcgtac accgcctctg tcaggatctg tctgcacgaa attttttgcc acgttttgag
    11401 gataagtata attcccgccg atctcgatat tttgcggtat ctcattttcc tcgtggatcg
    11461 tatatacagt ctctttcgga ataccgtaca ctgctacgct tgcagtctgt tttcccgccg
    11521 gaacagttac gtcgatttcc tgaacaatac tgcttccgct ttccgacatg cttcccgatc
    11581 cgagatttcc ttcaaggctc attccggcta cgtcagtata atgaacatga aatttatatg
    11641 tatgatcttt tttcggatcg atctgatcga cggtttttgt aatgatcagt cctccgctga
    11701 tcagatcgtt tttatacgcc acagtcagat ggatcccgtt tttctgaagg ttgtcaggat
    11761 cgagatagct tctgaaaaca aaagtctcgt tcttttccga tacatgtcct tctctgtttg
    11821 aactcggata gtgataccct tttccgtccg cgctctttac atttctgtcg tctgacgccg
    11881 tatatgttct tacatccgtc actgtattat tcaaaatctc cggatcattt tccaccgttc
    11941 ttgccgtcgg atgcagcgca agaatgttat ccgcaatata accttccgta tacattcccg
    12001 acgcaccgtc atataccgca ttttccaaaa tcgaccatga agtccgaaat actttgtttc
    12061 tcagcggatc gtcttgtgtg atctgcaaat aacttccctc acggaattta tcgacaaaag
    12121 acgctttgtt tccatctgta agctctaaca gtcctctcga tctgaccatg aggtcatttc
    12181 cgttgttcac aacatctgtt tcctgtatcg gattcaaccg gaagaacgct ccgcttaccg
    12241 gatttaagac cgcctcatta tagctaagag gccgctcttt tgttcccagc gattcataat
    12301 ctgaaatctg atcgtcgctt gcataaaatc cggtcgattg gttcggaatg atctttcctt
    12361 taaaatccat atccgccata tacagacttc ctctgtcacg tgcaaaggcg aactgcactt
    12421 tggcgacatt tttcatatca aaatccgata ttcccgaatt ccagtctgcc agattcagac
    12481 gaacgatcga ccactggcct ttcggcatcg aattactgta tccgtaatac gtcatctgac
    12541 tggcaggtct ggtgatcaca tttccgtgcc ggtctgtcag tctcgctttt aacgaccacc
    12601 cggtagcgct ggaagcatcc gagttattat aaactgacat ttgaagatat gccgcttttg
    12661 acagatccac actgcctccg ttctccgcac agtcgatcgt tgtccagtat tttcccgaag
    12721 caatttcttc cgacatctga tactgatcga gatttttgcc gctgtcttta ttcacaagct
    12781 ttatcacatc gctgcgctca ccgataatcg ggccgtctgt ctctgcgtcc ggatctgctt
    12841 ctttgagctc actttctccg gtcttagacc ctctgtatgt tccgctgttt ttaaaatcgt
    12901 taaacttaac agcgccttcg ttacggagat aacctaccga atcttccacg ggaatctgcc
    12961 ttccgcttgt cgcctgattc aatattttat aagtaaaact tccgatatat cctaatgctt
    13021 ttccgaatac gtcctgattt gccgaagcag tgcttacctg attcgtcaga tcaagctgat
    13081 tcgttttcgg gaaattaaaa cggatcttta agttggaaac acccgctccg cgctccgcat
    13141 aaaaaatcgt gatcttatgt atgccttgcg aaagttcttc catatctaaa cacggaccgt
    13201 tcgcatcatt tttttcataa atattcgcct gtgcatgatg ctgattttta ttgatcggat
    13261 atccgtcctc tgttgcaccg tctttattta atccgtacac atctaccgct ccggtctcaa
    13321 agttaatatg accttccaga ggattatgtt tccctgccat atcaagtaca agttttccgt
    13381 cgatatacaa taagaaatca tcgtctcccg aaaactcaaa ttcaatcggc ttctcaatat
    13441 attctcctgc cttttctccc ggcatacgca cttttccgtc ttccgtcagc ttaaattcca
    13501 gttccatctt cataccgaag cagaaattcg ttttcatcct atccaaccgg taaaacgagt
    13561 tttctttcgt tgcgtcgttt ttgtcattaa acgggaaaaa tccgaattga tcttttccca
    13621 ttgcgacatc tctgtcacct gttttcagcg cgtcttcctt ttctcctacg tctaaataat
    13681 agcctttatc gggatcttct tttaaccgca gcacatgatc ggacttcaaa ctactgtact
    13741 cccaatagcc gtcatctgca aggaaaaacg gaaatgaaac gttattatac acgtatccca
    13801 gcgccgagtg aaacgcattg tttccgcgaa gaaaactctc gtcaaactgc ggaagtttca
    13861 cacctgcagt tgagattttt ccatcttcca gttgtttatc gacatgcccg taagcatact
    13921 ttaacgtatt tcccgaatgc cagtcattcg gtccgatctg atttcccgac atattgcccg
    13981 gagaagaatt atttagcgga ttatccccgt ctgttacagc cggatgcctg atatattttc
    14041 cataatacca cgccgccgct ttttcatttt tcccttggaa atacttggag atgccgtcgt
    14101 tcaatattcc gaaactcgtt tcatattcgt acggcgatcc gtttacttcg ctcaatcttt
    14161 ttccttgaag ttcataatcc gaataataat cataaaacgt cgtatttgcg taatagtatt
    14221 ttggatcata cgacttgttt ttcgctatcg gaatgttctt tccctcatct ccgtgatcgc
    14281 tcgccttaaa gatcgaactc catgttccgt tgattttcga ctgctcactc gtataactta
    14341 aacttttctt ctgaagtacg tctgcgaaaa agcatggaaa agcataagta tcgtcaatct
    14401 cagttatatc cgtcttatat tttacaactt tgccgtctgc tctgttttca catatacgaa
    14461 attgaatata cttttcttta tccgacagtt tttcatgtgt aaaaaatgtt ttataaaaaa
    14521 cttcattttc catatcttcg cttgcctgac ccgttcccga agattccggc agcgcctgat
    14581 ccatcttttt ccattcggtt tccactgcct gcccgtcttc cgggttttcg caaagacgat
    14641 attccaccgt atccgccgct tctgttttcc cgccttctac gccacttgca acgagcgctc
    14701 ccgacgttcc gtacatctga tctttaaacg cgacataccg gtcgccgtct gtttcataac
    14761 ttctccaaat acgatgactg tcatccaagt tcctcagaat gaaaaagtta tcgcctgatg
    14821 caatattata taactgataa tatactttat cgtcttcttt attatcggaa ggatgtttga
    14881 aaccgccttt ttcattaagc tctccgtttg aaattgccag aagcgacagt tctgtcagac
    14941 ctgatccttt tccaaacgca taataagtac ataattcata gcgggtctct cttgtaactt
    15001 tctttgatac gatctttccg tttttatctg cgtaagatac ccataactca tcccacactt
    15061 tatctgtttt cgtatcataa tttccgtctt tgtcattatc aacatagatc gccagttcat
    15121 ccaagatgtt attacttgat gacggatgac ctccccagta ggacgtgtga taggacatgt
    15181 cccagtaaaa gcagtctctt gtcttttgcg taaatacaaa agatcccgat cctctttgct
    15241 ttacctctcc ggcggcgtct attaccggct ctttttttat ctcatgataa gggtcgttcg
    15301 gatttgcggc ctgacgcaga ttgtatacga tctgagagtc tatttctctc gcttctccgt
    15361 ttttattcgt tacttccatc tcaaacgcaa cataagaaca gccttccggt attaccgttc
    15421 cgaacagtcc cgcttcgtcg acgctgatca ttcgatatgt cttcggtatt tcctttttcc
    15481 ccgcattccc cggttcaatc ggatcttctt tataaaacaa tgcgtagact gtatttacat
    15541 gcagtttctc aaacgttgtc gctccgccga acgcacttgc ctcatacaag ttcgggatac
    15601 ttgcaaaata cattgcttca tataccgcgc tgtcttccac tgttctgtaa acatccgtct
    15661 ctgtcatccg ctccccgaga gcacagagcg catgttgcct cccatccaga tcataataga
    15721 tgtgagaaca atattcatca ttgcacaagc tttcctgctg cgcagatgtc atgttctctt
    15781 cttccgatga tccttcagaa accgttctat ccgatgcatt cggactgttc ttcgcgtctg
    15841 tctcatcaga cgtctgcccg ttttgccgta cagcatcgtt ttcagacgtc tgcccgtttt
    15901 gcccattctc ttctaacgga tatagggcgc tcccatctat tccgtcggac agactttctc
    15961 cttccttccc cacctctgcg gaagggtttt ctgttgtaat ttcacctggt tttgccgaga
    16021 cttccataat cggccggatg atcataacga tcaacataag caccgccaag atttgcgatg
    16081 ctttcagatt ccccttaaat attctgtttt ttctccccat tatacaccct cgtacttatt
    16141 agtattatac ttatctgtac aagttcccca accttgtacg aatatatccg tattctatca
    16201 ctcgcatata ggaattacaa ccctgccgcc tgatctattt aagttttcct catttttgtg
    16261 atatttttct atatttttcc acatccgctc tttattttcc ctttttttcg ccgctttctt
    16321 tcgtcttttt gcacagcatc caaagatatt tttcgtattc tgctccttct ttccccgaaa
    16381 ttccaagcgc tgccgactcc ctaacagcct tctcgatcat ctctcccgcc agtctgaccg
    16441 aatcttctgt acggtctccg cgagcctttc cgcctgcaat tacggaagcg aacagatctc
    16501 ctgttcccga gtaacttgcc ccgataaaag aagagatccc tgccgttgtt ttctcacgcg
    16561 tcaccgtaag atttgcgacc ttcctgccgc ctgttccggt ctcttttccg actgtacact
    16621 ctttcgcagc gtcctgctcc tcaaataaca cccctgtcac gatcacttcc gatacgccac
    16681 tgtccaaaag tctgcctgcc gctttcgaca cttccgaaaa aagagcttct ctgtctcccg
    16741 tttccgaaat attttgaatc ttatcataat cataatccgt caaaagacaa agttccgtca
    16801 cattcggcgt cacgatatcc gctcttttaa caagctgttt catctctttc tgaaataacc
    16861 cgtcaaaaac agaaaatcgt tttccgttat ctccgagcac cgggtctaca agaagaaatg
    16921 tgccttcttt gtgaaacaga tcgagaaagt gattcacttc cccgatctgt ctgtggtctg
    16981 acataaaccc tgtaacgatc ccgtcaaact gcgcgcctga tttcttccat tcttcggtaa
    17041 tcatccccat ctgctctgta agatcctgaa aaaaataact ttcaaaccct gtctgtgcac
    17101 tcaaaacggc tgtcggaaga ggacacggct gcacccccat cgccgacaat accgagattg
    17161 cggctgtcag cgaacatctt ccgaagcctg ccagatcatt tagaaccgct actttccctg
    17221 ccatgttttc tctccctccg aaataggttt cattacgatt gcggaatgac gccccatatt
    17281 gatgacgatc tctccgtttt ctacaatgaa ctcgtcatac tctgtcgtat atccttcctc
    17341 atatgtatac ataagccttt ccatcctgcc ttgcttcgga actcccgcaa accagaccgg
    17401 aactgtcagc tcttttaaat ctttactgtt gtttaagata acaatgatct gctcatcttc
    17461 ctgaaatctt gcgtatgcaa gtacgtttct ttccgcatag agcagcttga tcgacccttt
    17521 cctaagcggt ttttcctgtt tatggattcg gatcatctct ttatgaaacg cgagcagttc
    17581 tttattctcc ctgccccacg gatatgtgcg cctgttgtcc ggatctgtaa atccgcatac
    17641 tcccgcctcg tctccgtaat aaacggtcgg cgctccgacc catgtcatct gaaccgtaac
    17701 cgcttcacgc ataacggcat agttaacgcc ttcttccgca gctttcgcac cgacatgttc
    17761 ggcgcgtcct acgatgtgat tcgtccttgt caggaaacgg gagtgatcgt gattggacag
    17821 ctcattcatc gcgacctgca aagaaggagt gagcatactt gccataaaat ggcgcattcc
    17881 gcctatgaag ttgtctgcat ttccccacag gtctgttctt cgctcgtcac tgtgtttttc
    17941 cattccggtc aaaaaccacg tcaaaggttc cataaacgcg tcataattca taacgctgtc
    18001 ccattcgtcc ccctgcagcc attctcccgg atcgccgtaa tgctctgcca ggatcaaagc
    18061 ttcgggattt gcctctttta cttctttgcg aaaacgcttc caaaacatat ggttatattc
    18121 gttgctgcac cctaaatccg ccgcaacgtc aagtctccat ccatccgcat tatacggagg
    18181 cgaaacccat ttctttccta tatccataat atattgctca agttccgggg aatcctcata
    18241 agaaagtttc ggcagcgtat catgtcccca ccatccgtca taacttttat tgtaaggcca
    18301 cgcctctttt ctttcatcat gaaaacggaa aaacttccgg tacggacttt ccccgctcac
    18361 atatgcccct tttggatatt ccggctgctg ttcatagatc cgctccctgt caatccattt
    18421 gttaaacgat ccgcaatgat taaatacgcc gtcgagaatc acacgcatcc ctcttttgtg
    18481 catctcctcc acaagccttg caaacagctc gttgctcgct tcaagattgc ggatatcgcc
    18541 tgttcgcttc tgatattttg tagcgcctgt attatctttc gctcctttag gaagcgtctc
    18601 tccgccgtcc gagacgatct ttccataatg tggatcgata taatcataat cctgaatgtc
    18661 atatttatga tttgacggag aaacgaaaag aggattaaaa tatacaacct caacgccgag
    18721 atcctgaaga taatccagtt tatccatgac gccctgaaga tctcctccgt aaaaattacg
    18781 gatatccatc gccgccggaa ctttactcca gtctttgatc tttacgctcg gagcgccgat
    18841 ataaatatat tccccgtcct ccacgtcatt tgtcggatcg ccgttacaaa accgatccac
    18901 aaaaatctga tacataaccg cgccttttgc ccactcggga gtagaaaatc ccggaacgat
    18961 cataaacgca taaaaatctt ctctctgatc ggaaacgccg cacctgctga aataacatat
    19021 ctgatctccg ctttttactt caaacagata actgaacgga acatcactta gctgccattc
    19081 tatctcatag aaatcgaatt cgccttccga aacaatcttc cacatcggat acgacgaatt
    19141 gccggtcaaa atatggactt cttccacatc gttgtgagcc gtgcgaaacc gaatcttgat
    19201 cttttcgttt tccttcggtt ctgcagggat gacataatcc tgcgtaccgt cacagaatag
    19261 tgcctctctg ttcatatatt acccctcctc cgacactccg tcctcaaaca atgcctcaaa
    19321 ctcgcttcgt gtgatcttct ctttttcaag cagaagtttt gcgcacgcct caagaacatc
    19381 atggtattct tcaagaatag aacgggcttt ggcatagcat tcgtcaataa tacgttttac
    19441 ttcctcgtcg atcgttcccg cgatcttttc tccgtatcct cttgacgtat gcccgaagtc
    19501 gcgtccgata aacacttcgt cactgtcatt gtcgtagttg atcaatccga ggcgttcgga
    19561 cataccgaac tttgtaatca ttgattttgc gatcgcagtc gcctgtttga tatcctgcga
    19621 agcgcctgtc gtaatatcgt caaatatttc ttcttcagct acacgaccgc ctaaggaaac
    19681 cgtaatttcc tgaagcatct gcccttttgt attaaacatt tcgtctttct ccggaagagg
    19741 catcgtatat ccccctgccc cgccggtcgg aatgatagac acactgtata caggtcctac
    19801 atccggaaga acgtggaaca aaatcgcatg tcccgcctcg tggtaagccg taatctttct
    19861 ctctttttca gatacgatgc ggctcttttt ctccgctccg atgcctactt ttacaaacgc
    19921 atgacggata tctttctgtt ggataaacat tctgttatcc tttgcagcta tgatcgcagc
    19981 ttcattcagc aggttttcaa gatccgcccc tgtaaatcct gccgtcgtct gagcgatctg
    20041 tttcagatcc acatcttccg ccaacggttt gtttttcgca tgaactttca ggatctcctc
    20101 tctgccgccg acatccggcc ggcctacaag tacgtttctg tcgaaacgtc ctggtcttaa
    20161 tattgccggg tcaaggatat cttttctgtt cgtcgcagcc atcacgataa ttccctcgtt
    20221 gacgccgaat ccgtccatct caacgagaag ctgattcaac gtctgctctc tttcatcatg
    20281 accgccgcca agtccgctgc ctcttcgtct tgccacagcg tcgatctcat caataaaaat
    20341 aatacacgga gcatttttct tcgcatcctg aaaaagatca cgcacgcgag atgcgccgac
    20401 acctacgaac atttccacaa aatccgaacc cgaaatactg aaaaacggca ctcccgcctc
    20461 tcctgcaaca gcttttgcaa gaagtgtttt tcctgttccc ggaggtcctt ccaacaaaac
    20521 gcctttcgga atacgagcgc ctacctgcac atattttttc ggcgctttca aaaaatcgac
    20581 gatctccgca agttcttctt tctcttcctg tagtcccgct acatttgcaa acgtcacctt
    20641 tatctcgtca tgatttgtca tgcgggcgcg gctttttccg aagttcatcg cttttgcatt
    20701 cgccccgttt ccctgacggt tcataagaaa gaaaagcagc atgatcccgc caagcgtgat
    20761 cacggtcgga agaagaacgg aagaaagcat cgacgtttcc ggaattgcgg atacctgata
    20821 taaaacttcg tttttatcaa gcagtttctc tactttttcc acattcgaca cattgaccga
    20881 acggttctca tctgtatctt tcagagcaaa cactaccgta cctgtcggaa ctgccttatt
    20941 ctgatcgata tatacttctg ttacattttc tgcctcaacc tgcttaacaa actctgtgta
    21001 tgtcatctcc tgtcgatgca tctgcatccg gtttgccatc cagagcgcgc caaaaacgat
    21061 caccataaat ataatgatcg taaatccatt cacacttctg taatttctgt tattatccaa
    21121 aactgatcct cctgattatt ccacgccttc cactacgccg atatacggaa ggtttctata
    21181 tttttgggcg taatcaagcc catatcccac aacaaattca tccgggatct caaatccgca
    21241 gtaatcaacc tttacatctt tcactctgcg gtccggctta tccaaaagcg tacacaggcg
    21301 catacttgcc ggatttctct ttttcagcac atcgatcagg taatacagtg tattcccgga
    21361 atcaatgata tcttccacaa taagcacttc tttattctcg atcgactcat ccagatcttt
    21421 cgctatacga acaactccgc tcgaagccgt tccgtctccg taacttccga cacacataaa
    21481 atccatagaa accggcacgg taatccgctt agccagctca cacataaaaa atacgccgcc
    21541 ctttaacaca cagatcaaat gaatttgttt tcctgcgtaa tcttcactga tctgtcttcc
    21601 gagttccctg atcctctcgt caactttctc ttccgaaatc atctctctga tcgtttctgc
    21661 catctttttc ttcctccgta atctttatct caacaatatt ttttgtcttt tctgtcactt
    21721 tgtacgctct gctcatcctg tatcccggta tccacacaat atggcttccg tctgcgacaa
    21781 gaagcatcct gtctctttct tccttcggga tcttctcgtt gataaaccat gctttcagtt
    21841 tctgacgtct cccctgatca tcgatcacga gataatcgcc gctctgcctt gtccgtacgc
    21901 aaagaccgca tttaattata tcataatcaa tccatttcgt gtaacttttt tgcgggattt
    21961 cttctgcctg caaaatgtct ccgttttcca caaaacgcag ttccaacttt ccggcggcgt
    22021 ctgactccgg cgccgctttt cccgtttccg ccctgctttg tgtttgcctt atgatctcta
    22081 tcccgtcata tgtcctcttc gccgcaacgc ctcccggcag atcgatctgt tttcccacct
    22141 gccggtcaaa caattccgaa accgactgaa ggtgggcaga cgtcagatct ttctgtcctc
    22201 cgcgcacttt accgatacat ttttgcagaa gcagttttct cactgccgga aaactttgaa
    22261 aaaagctctc tttttctatg acaagttcta tcggcgattc cttctgttct tggtcctttt
    22321 ttctcacgct gacgcaggtt ttccacaact gccgcacgct gtaatcgaga tattcacata
    22381 tttcttccgc ctgacgggat aacgattcca gatgtttcac cgtctgatcg tttacttcgt
    22441 tttccaatac cggcagcact ttatgccgga tccggttgcg tgtataatcg tcctcctcat
    22501 tcgttgcgtc gacgcgccag acgattcctc tctcccgcag ataagactcg attctgtctc
    22561 ttgtaagtga aagaagcggt ctgatccggt tgtccctgac aggcacgatc ccgcaaaggc
    22621 cgcgtattcc tgttcctctt gctatattaa gcagaaccgt ctccgcctga tcgtctctgt
    22681 gatgcgcggt tgcgatcttc gttcctccgt tttcggcaca catttgttca aatgcttctc
    22741 ttcgtacttc ccttcccgct tcctcacaag attgtttccg tttttcagcg attaattcca
    22801 cttcttttcg gaaaacacga cactccactc caagttcgcc gcaaagcttc tttacatact
    22861 cctcgtccgc atccgcagcc gctccgcgca ggcagtggtt cacatggcac acttttacct
    22921 cgaaaccgaa cttttcccgc aaactgcaga gcgtcaaaag caggcatacc gaatccgctc
    22981 cgcccgatac gcctgctatc acaacatctc catgttcgat catatgaaat tgtacgatca
    23041 attcctctat taatttttct gtttctcttc ctgctttttc catcataagt atactatacc
    23101 aaatccgtgt ttttatgtat gaagcgccgc gcaatacaca gcttttcact atacgcatac
    23161 tataccgaat ccatccgcgc ctgtaaagta ttcctgtcac atatttccca cattcccaca
    23221 acgatcaccg tcatatcatc ggcaggctct ctgcctgtcc acgccaggac ctgttccagg
    23281 atatgatgcg ccatttcttt cggattgact atagcgcttc cctgaatgat cgtttcaagc
    23341 aatatatcct gctctcctac cggaagggcg tcaagaatcc cgtccgtaac catgatcaca
    23401 aagtctccgt cctcaagttt tctcctgaca gaatcaatct ctatatgatg gacaaccccg
    23461 atcggcagac tcgtagaact tagatgctcc acacagtctt ttttcttaat aaatgtcgag
    23521 gaagctcctg cttttacgat ctcgcattct cccgaataca gatcaaacac actgagatcc
    23581 acagtcgaat aatgaatctc ctcccgccct gtcgccagcg ttgtattgat catctgaacg
    23641 gcggcttttt ccgggaatcc cgcggcaagc aattcttcca aaagctcgat cacaagcgtg
    23701 ctctccttac atgccgtctc acctgcgccc atcccgtcag aaagagccgc cgccttcttt
    23761 cctcccggca gttccatgag tgtaaaatta tctcccgata ttttcgcaca tcctttcccg
    23821 atcttggcag tacctgacag cgtatagtac gacggtccct ctctgcatac gatcgtcata
    23881 taacggaatc cgactacctg cggacttcca tgtgcaagga caaaacgtct tcccgctgct
    23941 tctgatacgg cctgaacgac ttcttttgcc gtccctttct ctttgcctgt acttcgtact
    24001 gtcacatgta cttcaaactt tccttcgcca tttaagaaca aatatgtata caaaactcgc
    24061 attccacatt ttctaagcgc ccctgctatc ctcttttcca tgcgctcatc cgtaaaaata
    24121 cttttttcca attcccgcac tgtctgtttc ataatatcgg caaaaagatc catctgaaac
    24181 gcacaccctt ccctgcttcg cgccagacgg tttgtccata tgatcgtttg tctcgcatcc
    24241 ctgaaacctt ccagcatctc ccgcaaaaat ctcggagctt tctcgcatct ttgcatcagt
    24301 tttctctttg tctctacgtt taattcattt ccataatgat cgaccgccga gagcacgtca
    24361 tatcccatct ggtacgtatg aacaaagttc tctccccagc accacgcgtt cttttcacat
    24421 tttttgcaga ccctttcttt cacccggtca aacatcccgt caatatcggc tcccgaaaac
    24481 tcttctttgt gtgcctctaa ctctaaaaat gtatgggaaa gctgtttgag cgaacttgca
    24541 ta
//
LOCUS       DBMDXF010000003        23571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_711_length_23571_cov_2.219344, whole genome shotgun sequence.
ACCESSION   DBMDXF010000003 DBMDXF010000000
VERSION     DBMDXF010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_711_length_23571_cov_2.219344"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(251..1582)
                     /locus_tag="ACZPD4_00240"
     CDS             complement(251..1582)
                     /locus_tag="ACZPD4_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1377539.1"
                     /translation="MNIQLSDHFTYRRLIRFTISTVLMLICTSMYSIVDGLFVSNYVG
                     KMPFAATNLIWPVSMGIAAIGFMLGTGGSAIVAKTLGEGKKEKANQYFSMILYFGIIV
                     SVIFSVICFIFTPQIARMLKADGELLEHCIVYGRILFATQTAFILQNMFQSFLVAAEE
                     PVLSLKINIAAGLTNVVFDYLFIAVFEWGVAGAAIATGMGQIVGGIAPFFFFAKGSRN
                     GLRITWRTKLYKDILLKTCMNGSSEMVTNLSTSLVNILYNFQLMKLAGADGVAAFGVI
                     MYVNFIFVAVFMGYSVGSAPIVSYHYGAGNDAELHNLYRKSKNILIVAGIVLTIAAEI
                     LSMPLVRIFVNYDKELLLMTVRGFRLFSFSFLIMGINIWASSFFTALNNGFVSAAISF
                     LRTFLFQVAAVCFLPLIIGIDGVWTSVLAAETMALAVTIIFLIKEKKKYRY"
     gene            complement(1579..2070)
                     /locus_tag="ACZPD4_00245"
     CDS             complement(1579..2070)
                     /locus_tag="ACZPD4_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1377540.1"
                     /translation="MEGIRMYKEKQKVGFGMNYTQSRELKENNRIYREMESICHEICV
                     KAGISDTDFIVLYSIVEFEDGCCQREIAKQYSISRQTVNSSVKKLVQNGCVVLESGKG
                     HDKQIVLTEKGKAMAKQKIIPVMEAEAGVFMHMTEEERKKLLRLNAKYMEIFRQKVGE
                     LRL"
     gene            2361..2855
                     /locus_tag="ACZPD4_00250"
     CDS             2361..2855
                     /locus_tag="ACZPD4_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1377541.1"
                     /translation="MRIRKATQQDLHSIMQLYENARAFMREHGNPGQWGTTYPPQNMI
                     ETDIRFGNLYVCIEKDTIEAVFFYKDGIDPTYLKIYDGQWKNDAPYGVVHRITSSGKV
                     KGAASFCLDWAFSQCGNLKIDTHENNIVMQNMLKKNGFTYCGRIYIDNGGERLAFQKD
                     SPVP"
     gene            complement(2883..3512)
                     /locus_tag="ACZPD4_00255"
     CDS             complement(2883..3512)
                     /locus_tag="ACZPD4_00255"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1377542.1"
                     /translation="MRNIVITISRQYGSGGKTIGAMLAKELGINCYSREILRMASEDS
                     GINEKLFGMSDEKVRKAGWFKLLSRPYEGGLIPPEDREFTSDDNLFNYQAKVIKELAA
                     KESCVIVGRCADYVLKDYPNVVSVFVHADRKFCLDRSMERHSMTEKEMQKYIEKTDQY
                     RGDFYRYHTGHEWVDARNYDLCLNSGKLGFEKCVEEIKAYIKVRFGETL"
     gene            complement(3528..4238)
                     /locus_tag="ACZPD4_00260"
     CDS             complement(3528..4238)
                     /locus_tag="ACZPD4_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377543.1"
                     /translation="MAMLEVKDLQVYYGMIQAIKGISFEVNQGEVIALIGANGAGKTT
                     TLHTVTGLISPKSGQVLFEGKDITKTPAHKIVSMGMAHVPEGRRVFAELSVYENLRMG
                     AYTRKDKAEIEETLKSVYKRFTRLQERKNQMAGTLSGGEQQMLAMGRALMSKPRIILM
                     DEPSMGLSPIMVNEIFDIIRAVSESGTTVLLVEQNAKKALSIADRAYVLETGKIVLSG
                     DAKELLEDDSIKKAYLGE"
     gene            complement(4250..5008)
                     /locus_tag="ACZPD4_00265"
     CDS             complement(4250..5008)
                     /locus_tag="ACZPD4_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377544.1"
                     /translation="MALLEVKNLGISFGGLRAVNGFDISIEKEALYGLIGPNGAGKTT
                     VFNLLTGVYKPDSGKVLLDGTDITGKNTIEINKAGIARTFQNIRLFKQLSVLDNVKVG
                     LHNKHHYSTLSGVLRLPGYRKVEKEMDEQAMELLKVFNLEDVAHVAASNLPYGQQRRL
                     EIARAMATEPKLLLLDEPAAGMNPNETGELMDMIRFVREKFHMTILLIEHDMKLVSGI
                     CEKLTVLNFGEVLCEGKTSDVLNDPQVVTAYLGE"
     gene            complement(5008..6075)
                     /locus_tag="ACZPD4_00270"
     CDS             complement(5008..6075)
                     /locus_tag="ACZPD4_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1377545.1"
                     /translation="MDKLKNMKKTTKNNIITYALVIAAFVIMQSLINAGSISSLLEGL
                     MVPLCIYIIMAVSLNLVVGVLGELSLGHAGFMCVGAFASAFFSKCMRDTIESASLRFF
                     LALLIGIAAAAIFGILIGIPVLRLKGDYLAIVTLAFGEIIKNLVNVLYIGKDSNGFHF
                     STKDVMALNMEPDGKVIINGPQGITGTPKDATFFIGFILILITLFIVLNLIHSRDGRA
                     IMAIRDNRIAAESVGINITKYKLMAFTISAAMAGAAGVLYAHNLSTLTANTNNFGYNM
                     SIMILVFVVLGGIGNIRGSIIAAVILTLLPEMLRGLSDYRMLIYAIVLIVMMLFNWAP
                     KAIEWREKYLSFGKKKKEAVK"
     gene            complement(6088..6972)
                     /locus_tag="ACZPD4_00275"
     CDS             complement(6088..6972)
                     /locus_tag="ACZPD4_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1377546.1"
                     /translation="MSFISYLINGISLGSVYAIIALGYTMVYGIAKMLNFAHGDVIMI
                     GAYMALITMMKAGMSPILAIVAAVIVCTVLGILIERIAYKPLRNASSSLAVLITAIGV
                     SYLLQNTALLVFGANAQTFPSVIKWKGLSLAGGKLNISGETIVTIAACVVIMIVLMLF
                     VQKSKPGQAMRAVSEDKGAAQLMGINVNGTIALTFAIGSALAAVAGVLLCSAYPSLTP
                     YTGAMPGIKAFVAAVFGGIGSIPGAFIGGILLGVIEIFGKAYISSQMADAIVFAVLII
                     VLLVKPTGLLGKKIQEKV"
     gene            complement(7211..7855)
                     /locus_tag="ACZPD4_00280"
     CDS             complement(7211..7855)
                     /locus_tag="ACZPD4_00280"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1377547.1"
                     /translation="MMKHTIITIGRQFGSGGHEVGNRLAERLDIPLYDHNLIRMAAQE
                     LRISSEDATQVDETILGRFLTAYIVSTGNYTAFMSGDESGEPLSDLVFNRQSAIIRKL
                     AEKGPGIFVGRCADYILGDYSNCINTFIYAYKDDRIRRIMKLYELNEKQAADKIKKTD
                     RERKLYYEARTGREWGGIESNSMMFNTSLLGIDGAVDALEAIYRKWDAGRDVES"
     gene            complement(7945..10095)
                     /locus_tag="ACZPD4_00285"
     CDS             complement(7945..10095)
                     /locus_tag="ACZPD4_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377548.1"
                     /translation="MKKGFYIRLAGEGISKNRKLYFPYILTCICMIMMYYIVSFLSIS
                     KELASIRGGEMLQGLLSMGVFVVAVFALIFLYYTNSFLIRKRKKELGLYNILGMGKRN
                     LVRILLWENILTAVISLVIGIVFGILFSKLAELLAIKLLDGATGFGVHIEMKPILMTV
                     GLFLAIFLLIMIRMLVSVYKLRPIEMLRSENVGEKPPKANWLFAFAGAVILAGAYYLA
                     VKIIDPTTAMLVFFLAVVMVIIATYLLFIAGSVALCKLLQKSKKYYYKTRHFVSLSQM
                     VYRMKRNGAGLASICILSTMVLVTISSTMCIYADTESSIRNRYPHDITIEWSGKTSLP
                     QNEDGTDTELKDSVVQRLTPEMTMPYKEAVEKVIESHKVKAENLENYNLYSLSGVQSD
                     DVLSIDFEKMKGEISSINAGMSGSVKTMYFTTMDDYNKLKGTDKTLAEDEMMLYSYKT
                     DYEYDSFTIDGVGTWKVETMKEPPIGVGSAMANMQGSYQVVVKDLSVIEKIATAYETI
                     NAKPLQDGTLYLLSLKECYDFDLKCDEEKQIEIYKDILESFEHISEAADEQGNRMGDS
                     LANYYADSKAYGKGDFIALNGGLFFLGVLLGAVFLFGTVLIMYYKQISEGYEDQDRFN
                     ILMKVGMTKKEVKQTINSQVLTVFFMPLIMAGIHLAFAFPMIQKILMLMSGVEAKVFV
                     IVTIGCYLVFALFYVLVYVVTSKSYYTIISSKEK"
     gene            complement(10092..10856)
                     /locus_tag="ACZPD4_00290"
     CDS             complement(10092..10856)
                     /locus_tag="ACZPD4_00290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377549.1"
                     /translation="MAVLDVKNLKKIYRPRFGGNQVQALSCVNFSVEEGEYVAIMGES
                     GSGKTTLLNIMAALDKPTEGTVLLDGKPLSSIKDKDIAQFRRDNLGFVFQEFNLLDTF
                     NVKDNILLPLVLKGENYKHMDSKLQPIAKELGISALLEKYPYEISGGQKQRAAVARAL
                     ITEPRLILADEPTGALDSRSTDELLGLFARINAGGQTILMVTHSVKAASKAGRVLFIR
                     DGEVFHQVYRGQMTDEQLYQKISDTLTMLATGGDRR"
     gene            complement(10984..11976)
                     /locus_tag="ACZPD4_00295"
     CDS             complement(10984..11976)
                     /locus_tag="ACZPD4_00295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1377550.1"
                     /translation="MKRFLGYLYDCRYMWIWFILSGGAFVLCFALYEVELKAAVYPVL
                     LSAVFGILFFVLGYLRREKKYKRLFQISETGGIVSESLPKPDCMTEREYQKILLRSEE
                     EYRKQREEWQAARKDMNDYYATWVHQIKAPIAVMRVLLQQEDTPINRELTGELFRVEQ
                     YVEMALCYVRLGEGASDLLIKEYPLDDMIRKAIRKYAGQLIRRKLRVIYEGTDICVLT
                     DEKWLVFIIEQLLSNAVKYTVSGNVTITVDREKKQLSISDTGIGISPEDLPRIFEKGY
                     TGYNGRLDKKSTGIGLYLCRTAADKLGHKLTAQSTVGKGSSFTIDLSSYPLEVE"
     gene            complement(11973..12665)
                     /locus_tag="ACZPD4_00300"
     CDS             complement(11973..12665)
                     /locus_tag="ACZPD4_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1377551.1"
                     /translation="MYKILIIEDDLTMAQAIQKEMKMWGNDAQYVQDFKNVLETFAEF
                     DPHLVLMDITLPFYNGYHWCSEIRKISNVPVIFISSAADNMNIIMAVNMGGDDFVPKP
                     FDLSVLTAKVQAVLRRTYDLSGKVPVLEHQGAILNLNDASLTYNGEKIDLTKNEFRIL
                     QTLMENKGRLVSRDTLMTRLWETDNYIEENTLTVNIARLRRKLDKAGLTDFISTKVGE
                     GYIIRQERQVSE"
     gene            complement(12693..13115)
                     /locus_tag="ACZPD4_00305"
     CDS             complement(12693..13115)
                     /locus_tag="ACZPD4_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1377552.1"
                     /translation="MEELVVYLRKEYFRAVDLTDKSGHILEFKYRPKTVDVYEKGLSV
                     FHMEYVMENRTKVRVIEFYLTDFGLRGKGYGTACMNEIVTELGKKGITLIYAPTGYDL
                     APLGYTGPAQYKELMAGFYEKTGFTTSGDGDLMMKILD"
     gene            complement(13116..13874)
                     /locus_tag="ACZPD4_00310"
     CDS             complement(13116..13874)
                     /locus_tag="ACZPD4_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1377553.1"
                     /translation="MSEKETKVTKQETLAALRNPGELYVIMSAATKMPFVKCDEETFD
                     DEIFLYYQMEDAKDKARKLLDEKYVSAVAKLAKEQLLPFFTSLYIMGVNALAVNSGTD
                     MEITVQLSDLVTRNIPKELPEGKQIVENPALHLTAAYFMQELRKQEQPQMTEELKELQ
                     EELLAHYGKGTFLIPVEENGQIPILKQKDGSLYQPVFTDVLEFQKFTKGRPVRSAVVP
                     GEKIAELLMEDVKGVVLNPLGVNVQLQVARRKRE"
     gene            complement(13952..14350)
                     /locus_tag="ACZPD4_00315"
     CDS             complement(13952..14350)
                     /locus_tag="ACZPD4_00315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR1377554.1"
                     /translation="MEKNRNENNRKNPLNKSFGYAFEGIRTGIRKERNMKIHCLAVIA
                     VTAVGTFLHIKPVEWCICLLLFGLILSLELVNTALEAVVDLVTKEKKPLAKIAKDTAA
                     GAVLVSAIAAAIIGCIIFVPYLLELIKSKR"
     gene            complement(14367..15950)
                     /locus_tag="ACZPD4_00320"
     CDS             complement(14367..15950)
                     /locus_tag="ACZPD4_00320"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR1377555.1"
                     /translation="MSSIEPMEYNSRLKEIVAVLRKHSITKGVTPLKLREIIEDLGPT
                     FIKLGQIMAMHSDILPKRYCDELMQLCSDVKPMPFEEVLSVIEESYGRSWRRVFSHID
                     ESCQGAASIAQVHRATLKSGEEVVVKVQRRGIYEKMARDIELLRKAIKLMPPVSLKNM
                     ADFDLVLDELWTVTRDEMNFLTEASNMEEFARRNKDVHFVGTPKLYQQYTTVHVLVME
                     YIDGCAVDDKKRLKEEGYDLKEIGSKLVDNYIKQVMDDGFFHADPHSGNVKIRDGKIV
                     WIDMGMMGRLTERDREMLGKAIEGIALNDIGLIRQAVLGLGEFKERPDQRKLYEGIEG
                     LMLKYGHEDMGKINVGEVTVDLMDVMKVNKIRMPHGLTLLARGLTHMEGVLAAIAPDI
                     NMAEIAAARISGDFFRNFDWKKELKSSGKSAVKSMRKALDIPSMVSDMMAGYLKGQAK
                     VNLDLRASDDLAFLLRRLVRNIVMGLWVMALLISSSIICTTDMSPKILGIPALGAIGY
                     ILAFLIVLYVFIKHIFSRK"
     gene            complement(15953..16276)
                     /locus_tag="ACZPD4_00325"
     CDS             complement(15953..16276)
                     /locus_tag="ACZPD4_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR1377556.1"
                     /translation="MDKLSDNLKKIFLAGIGAVAVTAEKSKDLLDEMVEKGELTVEQG
                     KVLNEELKHNVKKTVKEKVNALKPASAEELSDLLDKMTPEQIAALKEQILKKEEASET
                     APDEA"
     gene            complement(16387..16944)
                     /locus_tag="ACZPD4_00330"
     CDS             complement(16387..16944)
                     /locus_tag="ACZPD4_00330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR1377557.1"
                     /translation="MGSIWHDISEERIFPTDFISVIEISKGSKKKYELDKETGYIILD
                     RILYTSTHYPMNYGFIPRTLGDDGDPLDVLVMCSEPLEPLTLVRCYPIGVMKMTDGGA
                     GDEKIIAIPWADPTYEAYTDISELPKHIFEEIKHFFTVYKDLEGKRTAVDEFEGALGA
                     VKIIEECIDRYKEKYGSGESDKTTE"
     gene            complement(17050..17472)
                     /locus_tag="ACZPD4_00335"
     CDS             complement(17050..17472)
                     /locus_tag="ACZPD4_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377558.1"
                     /translation="MKRKRDQRRCRGAAGKIPALIFCAAAVLCIVIWVMRHAQMQEGN
                     EPDVVASRCENNVYDLTVAANCDEIKDTEAFAEKVVQKYEENSFRTTKFSVDLGEDID
                     LVRFHVYLRREEIGEKEELFQIRYQDGDIILDGINGKR"
     gene            complement(17646..19274)
                     /locus_tag="ACZPD4_00340"
     CDS             complement(17646..19274)
                     /locus_tag="ACZPD4_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1377559.1"
                     /translation="MGITDVLALLGGLALFLYGMQMMSTGLEAAAGNRMKSILEKLTS
                     NRIKGVLVGAAITAVIQSSSATTVMVVGFVNSGLMTLKQAVWVIMGANIGTTITGQLI
                     ALDIGAIAPLFAIAGVGAIMFIKSEKVHHISSIFAGLGILFMGMDMMGAAMSPLKESE
                     AFISLMTKFDNPLLGILVGALFTAVIQSSSASVGILQALASTGMIPLSSAVFVLFGQN
                     IGTCITAVLASIGMKVNAKRTTVIHLLFNIIGTVLFTVICLVTPYVTWIEAMTPGDPV
                     AQIANAHTVFNIVTTLLLLPFGTHMANIAVRILPDSKKADDEDLRLKYIRPFESSYAI
                     GHSAVAVSQVRDEVNRMRDMVAKNISDSFDSLVQYDEKLRKKVSEREEYIDFLNKGIS
                     EYIVSLMASEMNMSDSRKINGYYAIISNLERIGDHAVNLAEYGDDMRKWEIDFSDTVK
                     EELNEMKAQCIAALDNLKEVTSENVERILSQAVIIEQKTDDMRDKYFKKQMQRLKKGK
                     CKPQSGIVFSEILTDFERMGDHALNIAQQYREME"
     gene            complement(19536..22664)
                     /locus_tag="ACZPD4_00345"
     CDS             complement(19536..22664)
                     /locus_tag="ACZPD4_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377560.1"
                     /translation="MKKRALRKDFYMEIRRSLGRFLSIFFIVAIGCAFFSGIRASEPD
                     MRYSGDAYFDNKNMMDLRIISTMGLTEDDVKAASKAEGIGHVEGRYSVDALLADGDNQ
                     IVVHVMSMLPTMNEIQLEDGRLPNKENECVVDVDYMEKSKLKIGDTITFSSGTDAEVT
                     DSLKTDTFRITGTVSSPEYIAFQRGSTTIGNGSVRAFVYVQEESFAMDVYTEICIQAA
                     GAKELTAFTSEYEDTVAKAKENIEKIKEQRQKARYTEIVDEANGKIAEAEAEVTDAQT
                     KLENGKAEAAAKLADARQKLENAQAQIDSGKVQLENSKAEVAATEQTLAQKQTEVQNG
                     TAALDQGIVQLNQQIEQLNAVKAQYEALKESGMTDEETLAALEQMSMQIQIGDAAVVE
                     AQAQIDQTRAQLQYAQGQIDNGYAQLEAARQQIAGAEAGIISGEQEIASGWEEYYAGE
                     AEANAEIAEGEQKIAEAQAELADAKAEVAELEKPKWYIYDRNDLPDYSGYGDNADRMK
                     AIGEVFPVIFFLVAALISLTTMTRMVEEQRTQIGTLKALGYARHSIAGKYLGYAFLAT
                     LLGSAAGIFTGEKIFPYIIINAYGIMYKHMNELLIPYNVMYGIGAAGTALFCTLAATI
                     LACYKELREQAAQLMRPPTPKQGQRVVFEKITFLWKRLNFSWKASVRNLVRYKKRFFM
                     TIFGIGGCMGLMIVGFGLKDSIFAVVDIQYGEIQRYDGTVIMAEGASEKEKGEVAQML
                     GKETEVDDAMEGILTQITIGNGSKWHDVYLDVPKDKKQFPEFVTLKERTTDRRYSLDE
                     KEVVLTEKIAKELEVQAGDMITIRDEEKGDLKVKIKAVCENYMGHYLYMTADAYEAVY
                     NEKPEYNAVFYKTVSGTTKEAQSVGEDILQLPGALSVSYTTDLKQQVDDMLGALDIVI
                     VVLTISAGMLAFVVLYNLNNINITERKRELATLKVLGFYPNEVSAYVYRENIVLTVLG
                     ALFGVALGKILHRFIIVTVEIDTVMFGRNIDMSSFVYSFLLTVAFSMIVNGAMYFKLK
                     KINMVESLKSVE"
     gene            complement(22705..23409)
                     /locus_tag="ACZPD4_00350"
     CDS             complement(22705..23409)
                     /locus_tag="ACZPD4_00350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377561.1"
                     /translation="MEEFVKLKDITKVYHMGEVEIRAADNINFSIKKGEFVVIVGPSG
                     AGKTTVLNILGGMDTATGGTLTVDGKDITAYDSRQLTGYRRDDIGFVFQFYNLIPNLT
                     ALENVELALQICKDPLDAKKVLEDVGLGDRLDNFPAQLSGGEQQRVSIARALAKNPKL
                     LLCDEPTGALDYNTGKAILKLLQNMCRERGMTVIVITHNQALAPMADRLIHIKNGQVS
                     HMETNENPMSIDEIEW"
BASE COUNT         6141 a         6325 c         4060 g         7045 t
ORIGIN      
        1 ttattcgttt cataccgaag cacatcatct gcgctttcgg ttcatgcaat gagcggttaa
       61 acagtccaaa tgatatcgta atttgtatct cattgccaca tgcgctagta taacatcacc
      121 cgaaggaaaa aacaaccatt ataacgagaa tccttctcaa aaatgccttt gttcttgatt
      181 tagtacaatt ttgcctgtat acgctcttat atttactata tattgtgttt gttaaatcat
      241 tatcatactt tcagtagcgg tactttttct tctcctttat taaaaatata attgtcaccg
      301 ccaaagccat cgtttctgca gccaaaacgg acgtccacac tccgtcgata ccgataatca
      361 gaggaaggaa acatactgcc gccacctgaa acaaaaacgt tctcaaaaac gaaatagccg
      421 ccgacacaaa tccgttattc aatgccgtaa agaaagaaga cgcccatatg ttgattccca
      481 taatcaagaa cgaaaaagaa aacaggcgga atccgcgaac ggtcatcaaa agaagctctt
      541 tatcataatt tacaaaaatc ctgacaagag gcattgaaag tatctccgcc gctatcgtca
      601 gtacaattcc cgctacaatc aaaatatttt tacttttcct gtataaatta tgaagctctg
      661 cgtcattccc cgctccgtaa tgataactta caatcggagc gcttccgacc gaatatccca
      721 taaaaaccgc aacgaagata aaattcacat acataatgac tccaaacgca gccacaccgt
      781 ctgcacctgc aagtttcatc aactgaaagt tataaagaat attcacaaga gacgtcgaca
      841 aattcgtcac catttccgac gaaccgttca tgcatgtttt cagaagaata tctttataca
      901 gtttcgttct ccaagtgatc cgcagcccat tgcggcttcc tttcgcaaaa aagaaaaacg
      961 gcgcgatccc tcctacgatc tgtcccattc ctgtagcgat cgcagctcct gccactcccc
     1021 actcaaatac tgcgataaac aagtaatcaa acacaacgtt cgtcagtccg gccgcaatat
     1081 tgatcttcag gctcaaaacc ggctcttccg cggcaacgag aaagctttga aacatattct
     1141 gcaaaataaa cgccgtctgc gtcgcaaaaa gaattcttcc gtatacgatg caatgttcca
     1201 aaagttcgcc atccgctttg agcatccggg caatttgcgg cgtaaagata aaacagataa
     1261 ccgaaaaaat aacgctgacg ataataccga aataaagaat catcgaaaaa tattgattcg
     1321 ctttttcctt cttaccttcc ccaagcgttt ttgctacgat ggcgcttccg cctgttccga
     1381 gcataaatcc gattgctgcg attcccatag ataccggcca gatcagattt gtcgccgcaa
     1441 atggcatttt cccgacatag tttgacacaa aaagtccgtc tacaatactg tacatcgacg
     1501 tacagataag cataagaact gttgatattg taaagcgaat cagtctgcgg tatgtaaagt
     1561 gatccgaaag ctgtatgttc ataatcttaa ttctcctacc ttttgtctga aaatttccat
     1621 atattttgca ttcaaacgta aaagtttctt tctctcctct tctgtcatat gcataaaaac
     1681 gcctgcctct gcctccatca cgggaatgat cttttgtttt gccatcgctt ttcctttttc
     1741 ggtcaaaaca atctgcttgt catgcccttt gccgctttcc aatacaacgc agccgttttg
     1801 cactaatttt ttcaccgaag aattcactgt ctgccgactg atagaatact gttttgcgat
     1861 ttccctctgg cagcaaccgt cttcaaactc tacgatacta tacagcacaa taaagtcagt
     1921 gtccgatatt cccgctttta cacatatttc atggcagata ctctccatct cccgatagat
     1981 cctgttgttc tcttttaatt ctctcgactg cgtataattc atgccaaacc ccactttctg
     2041 tttttcttta tacattctta taccttccat acttttatcc ttccctcctt tcaaaaatcc
     2101 gaaatcggat ttttctcgtt taattataat ccgatttcag attaaattca accattcatc
     2161 tatgatgtct ttaccatgag gtccactaaa aataactgtt tttctgttga gaaagaggaa
     2221 acattatgac ataatattat tataagtctg ccagaacaca aaacatcttg aacaagaagc
     2281 gccaaaaaca tgagaatatt tgaataataa aatacaaagc aggcgtcagc gctttttata
     2341 agaaagaaag gacttttatt atgcgtatcc gaaaagcaac acaacaagat ttacattcta
     2401 ttatgcagtt atatgaaaac gcccgcgcat tcatgcgcga acacggaaac cccggccaat
     2461 gggggacgac gtatcctcct caaaacatga ttgaaacaga tatccgcttc gggaatttat
     2521 acgtctgcat cgaaaaagat actatcgaag ccgttttctt ctataaagac ggcatagatc
     2581 cgacctatct gaaaatttat gacggacaat ggaaaaacga tgctccctac ggcgttgtcc
     2641 accggatcac ttcatccggc aaagtaaagg gcgcagcctc tttttgtctt gactgggcat
     2701 tttcacaatg tggaaattta aagatcgata cgcacgaaaa caatatcgtt atgcagaata
     2761 tgctgaagaa aaacggattt acttactgcg gacgcatcta tatagacaat ggcggcgaac
     2821 ggcttgcatt ccaaaaagac tccccagtac cctgaggagt ctcttgaagc tattctttta
     2881 agttataagg tttcgccaaa ccgtactttt atatacgctt tgatctcttc tacgcatttc
     2941 tcaaatccga gttttccact gttcagacac agatcgtaat ttcttgcatc gacccattca
     3001 tgacctgtat ggtatctgta aaaatcacct ctgtactgat ctgttttctc aatatatttc
     3061 tgcatctctt tttccgtcat gctgtgacgt tccattgaac gatccagaca gaactttctg
     3121 tctgcatgca cgaatacgct gactacattc ggataatctt ttaaaacata atctgcgcat
     3181 cttccgacaa taacgcacga ctcttttgcc gcaagctctt tgatcacctt tgcctgataa
     3241 ttgaacaggt tatcgtccga agtaaactct ctgtcttccg gcgggatcaa tccgccctca
     3301 tacggacggc tcaaaagctt aaaccatcct gccttgcgca ccttttcgtc cgacatacca
     3361 aacagttttt cattgattcc gctgtcctcg gaagccatac gaaggatctc tctgctgtag
     3421 cagttgatgc caagttcttt tgcaagcatc gccccgatcg ttttccctcc gcttccgtac
     3481 tgtcttgaaa ttgtgatgac gatatttctc attaaaacac gcctccttta ctctccgaga
     3541 taagcctttt taatcgaatc atcttccaga agttcttttg catctcccga aaggacgatc
     3601 tttcctgtct ccaacacata tgctctgtcg gcgatcgaaa gagctttctt cgcattctgt
     3661 tctacaagaa ggactgtcgt tccgctttcg ctcaccgcac ggataatatc aaagatttca
     3721 tttaccatga tcggagaaag tcccatcgac ggttcatcca ttaaaataat cctcggtttg
     3781 gacataagcg cgcgccccat agcgagcatc tgctgttcgc cgccgcttaa tgttcccgcc
     3841 atctggtttt tacgttcctg caaacgcgta aaacgcttat agacactttt taatgtttct
     3901 tcaatctccg ccttatcttt tcgtgtataa gcgcccattc tcaaattctc ataaacgctt
     3961 aattctgcga acactcttct tccttccggt acatgcgcca tccccataga gacgatctta
     4021 tgagccggag ttttcgtaat atcttttcct tcaaacagca cctgtccgct cttcggggag
     4081 atcagacctg taactgtatg cagcgtcgtc gtctttcccg cgccgtttgc tccgatcaaa
     4141 gcgatgacct ctccttgatt tacttcaaaa gaaatgcctt tgatcgcctg gatcatacca
     4201 taatatactt gtaaatcttt tacttcgagc attgccataa cgattccccc tattctccaa
     4261 gatatgcggt aacaacctgc ggatcgttca aaacatccga cgttttacct tcacagagca
     4321 cctctccgaa gttgagcacc gtcaacttct cgcaaatgcc gcttaccagt ttcatatcat
     4381 gttcgatcaa aaggatcgtc atatggaatt tttctctcac aaaacggatc atgtccatca
     4441 attcccccgt ctcgttcggg ttcatgcccg cagcgggctc gtcgagcagc aaaagcttcg
     4501 gttctgtcgc catcgctctg gcgatctcaa gccttctttg ctgtccatac ggaagattag
     4561 aggcggcaac atgcgccaca tcttccagat tgaacacttt caaaagttcc attgcctgct
     4621 catccatctc tttttccact tttctgtaac cgggaagtct taagactccc gaaagagtag
     4681 aataatgatg tttattgtga agtcctactt tcacgttatc aagaacactt aactgcttaa
     4741 acagccggat attctgaaat gtacgggcga ttcccgcctt attgatctca atcgtgtttt
     4801 tccctgtaat atccgttccg tccagcagca cttttccgct gtccggctta tatactcctg
     4861 taagaaggtt gaatacagtc gtctttcccg cgccgttcgg accgatcagg ccgtacagag
     4921 cttctttttc aatcgaaatg tcaaacccgt ttaccgcacg gagaccgcca aatgaaatcc
     4981 cgagattttt aacttccagt aacgccatta ttttacagcc tcctttttct tctttccgaa
     5041 agaaagatat ttttctctcc actcgatcgc tttcggcgcc cagttaaaga gcatcatgac
     5101 aatgagaacg atcgcataga tcagcatacg gtaatctgac aatccacgga gcatctccgg
     5161 aagaagtgtc aggatcactg ccgctatgat cgaacctctg atatttccga taccgcccaa
     5221 aacgacaaag acaaggatca tgatcgacat attataaccg aagttgtttg tattcgccgt
     5281 cagcgtagac agattgtgag cgtaaagcac gcctgccgct cctgccatcg ccgcagatat
     5341 cgtaaacgcc atcagtttat attttgtaat attaataccg actgattccg ccgcgatacg
     5401 gttgtcacgg atcgccatga tcgcgcgtcc gtctcttgaa tggatcagat taagcacgat
     5461 aaacaacgta atcagaatca gtataaatcc gataaaaaac gtcgcatctt tcggcgttcc
     5521 cgtaatcccc tgcggaccgt taatgataac ctttccgtcc ggttccatat tcagcgccat
     5581 cacatctttt gtcgaaaaat gaaaaccgtt ggaatcttta cctatataca atacgtttac
     5641 gaggttttta atgatctctc cgaatgccaa tgtcacgatc gccagatagt cgcctttcag
     5701 acgcagaacc ggaattccga tcagaatgcc gaagatcgca gcagccgcaa ttccgatcaa
     5761 aagagccaga aagaagcgca gtgaagccga ttcgatcgta tctctcatgc attttgaaaa
     5821 gaatgcactt gcaaacgcgc cgacacacat aaagcccgca tgtccgaggc tcaattctcc
     5881 aagtacgccg actacgaggt tcagcgacac agccataata atataaatac aaagcggaac
     5941 catcaatcct tcaagaaggc tggaaatact tccggcgttg atcagtgact gcatgatcac
     6001 aaatgccgcg ataacaagag catatgtgat gatattattt tttgtagttt ttttcatatt
     6061 ttttaattta tccatcgctc tgatcaccta cactttctct tgaattttct tacctaacaa
     6121 acctgtaggt ttcacaagaa gcacaatgat aagtaccgcg aatacgatcg catccgccat
     6181 ctgtgacgaa atgtatgctt ttccgaaaat ctcgatcaca ccgagcagaa ttcctccgat
     6241 aaacgctccc ggtatcgaac ctattcctcc aaatacagcc gctacgaacg ccttaatacc
     6301 cggcatcgct cctgtatacg gcgtcaggct cggatatgcc gaacacaaaa gcacacctgc
     6361 aacagccgcc agcgccgacc cgattgcaaa cgtaagagcg atcgttccgt ttacattgat
     6421 tcccataagc tgagccgcgc ctttatcttc cgatacagcg cgcattgcct gacccggctt
     6481 tgatttctgc acgaacagca tcagtacgat catgatcact acacatgccg cgatcgtcac
     6541 gatcgtctcc cctgaaatgt tcagcttgcc gcctgccaaa gacaaacctt tccacttaat
     6601 aaccgacgga aacgtctgtg cgttcgcccc gaatacaaga agcgccgtat tctggagaag
     6661 gtagcttacg ccgatcgccg tgatcaatac ggcaagtgaa gacgatgcgt tacgcagcgg
     6721 tttgtacgcg atacgttcga tcaagattcc gagtaccgta cacacgatta cagcggcaac
     6781 gatcgcaagg atcggcgaca tccccgcctt catcatcgtg atcagcgcca tatacgcacc
     6841 gatcataata acgtcgccat gggcgaagtt cagcatcttc gcaattccat aaaccatcgt
     6901 atacccaagc gctatgatcg catagacact tccaagactg attccattaa tcaaatacga
     6961 tataaaactc atgtctcatt ccctttcatt tctcttattg ttccgataaa cgatctcttc
     7021 tctgtttatc atgattaata aggattatac cacaaaaaat ataggattat caataagaaa
     7081 aaacgagtac gctcggtttt tttcacgtac ccgtttttaa ctgctttatt ttgtatctat
     7141 ccggcttttt cttccgaaat aaaaactccc ctgcatgacg tgtcattcat tttacatata
     7201 tgccggcatt ttatgactcg acgtctctcc cggcatccca tttgcggtaa atagcttcga
     7261 gagcatccac cgctccgtcg atccctaaaa gactcgtatt aaacatcatg ctgttcgact
     7321 cgattccacc ccattcgcgg ccggtccttg cctcataata aagcttcctt tcgcggtctg
     7381 tctttttgat cttatctgcc gcctgctttt cgtttagttc ataaagcttc atgattctgc
     7441 ggatacgatc gtccttatat gcataaataa acgtattgat gcagtttgaa taatctccga
     7501 ggatataatc cgcacatctt ccgacaaaaa ttcccggacc tttctccgca agtttcctta
     7561 tgatcgccga ctgcctgtta aagacgagat cacttagagg ttctcccgac tcgtctccgc
     7621 tcataaatgc agtatagttt cctgtactga ctatatatgc cgtgagaaat cttccgagaa
     7681 tcgtctcgtc tacctgcgtc gcatcttcac tgctgatccg aagctcctgc gccgccatgc
     7741 ggatcagatt atgatcgtaa agcggaatgt caagccgttc tgccaatcga tttcctactt
     7801 catgcccgcc gcttccgaat tgccttccga tcgtgataat cgtgtgtttc atcatatgtg
     7861 ccactcctct ctgcggatcg ctgtattgat atactcaagt ataacattgt ttcccgggta
     7921 tgaatagaaa tttattgatt ttttctattt ttccttactg cttatgatcg tataatagct
     7981 ttttgacgtt acgacataaa caagtacata gaagagtgca aacacgagat aacatccgat
     8041 cgtcacgatc acaaatactt tcgcttccac gcctgacata agcatcagta ttttctgaat
     8101 catcggaaat gcaaacgcaa gatgaattcc cgccatgatc agaggcatga aaaatacagt
     8161 aagcacctga gagttgatcg tctgcttcac ttctttcttt gtcattccca ctttcataag
     8221 aatattaaag cgatcctgat cttcatatcc ttcggaaatc tgcttgtagt acataatcag
     8281 cacagtaccg aacaaaaata ccgctccgag aagcactccg aggaaaaaca atccgccgtt
     8341 gagcgcaatg aaatctcctt taccgtacgc tttactgtcc gcataataat ttgccagcga
     8401 atctcccatc cgattgccct gttcatctgc cgcttcggat atatgctcaa aagattccaa
     8461 aatatccttg taaatctcta tctgtttttc ttcatcacat ttcagatcaa agtcataaca
     8521 ttctttcagg gaaagcaggt acaatgttcc gtcctgcaaa ggttttgcat tgatcgtttc
     8581 gtacgcagtc gctatttttt cgatcacaga caaatctttt acaacaacct gatatgaccc
     8641 ctgcatattc gccatggcgc tgcctacccc tatcggaggt tctttcattg tctctacttt
     8701 ccatgttccg acgccgtcga tcgtaaagga atcatactca taatctgttt tatatgagta
     8761 gagcatcatc tcatcttcag ccaaagtctt gtctgtgcct ttcagtttgt tgtaatcatc
     8821 cattgttgta aaatacatcg tcttcacaga tcctgacatc cctgcattaa tcgaggaaat
     8881 ctcccctttc atcttttcaa aatcaatact taaaacatcg tctgactgta ctccggaaag
     8941 agagtaaaga ttataatttt ccaagttctc agcctttact ttatgagatt caatcacctt
     9001 ttccacagcc tctttatacg gcatggtcat ctccggcgtc aaacgctgca ctacgctgtc
     9061 ttttaattct gtatctgttc cgtcctcatt ttgcggaagg gatgttttcc cgctccactc
     9121 gatcgtgatg tcatgtggat aacggtttct gatcgacgat tccgtatctg cataaataca
     9181 catcgtagac gagatcgtca caagcaccat cgtagataaa atacagatcg acgccagacc
     9241 cgctccgttt ctcttcattc ggtaaaccat ctgcgacaaa gatacaaagt gtctcgtctt
     9301 ataataatac tttttgctct tttgcagcag cttgcaaaga gcgaccgaac ctgcaataaa
     9361 cagaaggtat gtggcaatga tcaccataac aaccgcgaga aagaatacaa gcatcgcagt
     9421 tgtcggatcg atgatcttca cagccagata atatgctccg gcaaggatca ccgctcccgc
     9481 aaatgcaaag agccagtttg ccttcggcgg tttttcgccg acattctcac ttctgagcat
     9541 ctcaatcgga cgaagtttat ataccgacac aagcatgcgg atcataataa gcagaaagat
     9601 cgcaagaaac agaccgactg tcataaggat cggcttcatt tctatatgaa ctccgaatcc
     9661 ggtcgccccg tcaagaagct taattgccag aagttccgca agttttgaga acagaattcc
     9721 gaacacgatt ccgataacca gagaaatcac ggcagtcagt atattttccc acaaaagaat
     9781 tcttaccaga ttacgtttgc ccatcccgag aatattatat aatccaagtt ctttcttcct
     9841 tttccttata agaaacgagt tcgtataata cagaaagatc aatgcgaata ccgcaactac
     9901 aaacactccc atagagagca gtccctgaag catttccccg ccccggatag acgccagttc
     9961 tttacttatc gacagaaaag aaacgatata gtacatcatg atcatacaga tacatgtcaa
    10021 aatatacgga aaatacaatt ttctgttttt tgaaatgcct tcccctgcca gcctgatata
    10081 aaatcctttt ttcatcgtct gtcaccacct gtcgcaagca tcgtcagcgt atccgaaatc
    10141 ttctgataaa gttgttcgtc cgtcatctga cctctgtata cctgatggaa cacttctccg
    10201 tcacggataa acaaaacgcg tccggctttg ctcgccgctt tcactgaatg agttaccata
    10261 aggatcgtct gacctcctgc gttgatccgc gcaaacaatc cgagcagttc gtctgttgaa
    10321 cgagaatcaa gcgctcctgt cggctcatct gccaggatca gccgcggttc tgtgatcagc
    10381 gcccttgcaa cagcggctct ttgtttctga cctccggaaa tttcatacgg atatttttca
    10441 agaagcgccg aaataccaag ttcctttgca atcggctgca gctttgaatc catatgctta
    10501 taattttcac ctttcaacac aagagggagc aagatattgt ccttcacatt aaacgtatcc
    10561 aacaagttaa attcctgaaa aacaaatcca agattgtcgc gccgaaactg tgcgatatct
    10621 ttgtctttta tagaagacaa cggtttgccg tcaagaagga ctgttccttc cgtcggttta
    10681 tccaaagccg ccataatatt aagaagcgtc gtcttacctg agccgctctc tcccatgatc
    10741 gccacatatt ctccttcttc cacagaaaag tttacacagg ataatgcctg cacctgattg
    10801 ccgccgaaac gcggtctgta tattttcttc aaatttttga catctaaaac tgccatttca
    10861 tacatcctcc tcttttttac ttgatagtga aagtataaca aaagagccgg aatgccaaca
    10921 tcgaatttgg tgacattccg gcccgcctat gtgacaaatt tgtaaggcat ttccatgcag
    10981 cctttattca acttctaacg gataggacga cagatcgatc gtaaagctgc ttccctttcc
    11041 tacagttgac tgcgccgtca gcttatgtcc cagcttatcc gccgccgtcc ggcaaaggta
    11101 aagtcctatt cctgtagatt ttttatccag ccttccgtta tatccggtat aacctttttc
    11161 aaaaatacgg ggaagatctt caggtgaaat accgattccc gtatcgctga tactaagctg
    11221 ctttttctct ctgtctaccg taatcgtcac atttccgctt accgtatatt ttactgcgtt
    11281 cgacaaaagc tgctcaataa taaacacaag ccatttttca tctgtgagaa cgcagatatc
    11341 cgttccttcg tatatgaccc taagtttcct tctgatcaac tgtcccgcat actttcggat
    11401 cgcttttcgg atcatatcat ccagaggata ttctttgatc agaagatcgg acgcaccttc
    11461 tcctaaacgc acgtaacaaa gcgccatttc cacatactgt tctacccgga acaattctcc
    11521 tgtgagctct ctgttgatcg gagtatcttc ctgttgaagc aaaacccgca tcactgcgat
    11581 cggagctttg atctgatgta cccatgtcgc ataataatca ttcatatctt ttctggctgc
    11641 ctgccattct tccctctgct ttctgtactc ttcttcggat ctaagcaata tcttctgata
    11701 ttctctttcc gtcatacagt ccggttttgg cagactttcc gatacgatac cgccggtctc
    11761 actgatctga aaaagacgtt tgtacttttt ctcccgtctc agatacccta aaacgaaaaa
    11821 cagaatccca aacaccgcgc ttaaaagcac gggatatacc gccgctttaa gctccacttc
    11881 gtacaacgca aaacagagta cgaaagcgcc cccgctcaaa ataaaccaaa tccacatata
    11941 acggcagtca tacagataac ctaaaaatcg tttcattcgc tcacctgccg ttcctgtctg
    12001 ataatataac cttctccgac ttttgtggaa ataaaatccg taagtcccgc tttgtccagt
    12061 tttctgcgca gtctggcgat attgaccgtc aacgtatttt cttcaatata attatccgtc
    12121 tcccacagcc gggtcatcaa agtatctctg cttacgagac ggcctttatt ttccatcagt
    12181 gtctggagaa tacgaaattc atttttcgta agatcgatct tctccccatt ataagtaagc
    12241 gacgcgtcat tcaagttcag tattgctccc tgatgctcaa gaaccggtac ttttccggac
    12301 aggtcgtatg ttctgcgaag taccgcctgg accttcgccg tcagcacact gagatcgaac
    12361 ggcttcggaa caaaatcatc tcctcccata ttcacagcca tgataatgtt catattatca
    12421 gccgccgaag aaataaaaat taccggtacg ttcgatattt tcctaatctc gctgcaccag
    12481 tggtacccgt tgtaaaatgg cagagtaatg tccataagaa caagatgcgg gtcaaattcc
    12541 gcaaaagttt caagtacgtt tttaaaatcc tgaacgtact gcgcgtcgtt tccccacatc
    12601 ttcatctctt tttggattgc ctgcgccatt gtcaaatcgt cttcaataat cagtatttta
    12661 tacatgccgc cgctcccttt attcgtcttt tttcagtcaa ggatcttcat catcagatct
    12721 ccgtctccgc ttgtcgtaaa tcctgttttc tcataaaatc ccgccatcag ttccttgtac
    12781 tgcgccggac ctgtataacc aagcggagca agatcataac ctgtcggagc atagattaaa
    12841 gtaatccctt ttttgccaag ctctgtcacg atctcattca tacatgccgt tccatagcct
    12901 ttcccacgca gtccgaaatc tgtcaggtaa aattcaatga ctcttacttt cgtcctgttt
    12961 tccatcacgt attccatatg aaatacggac aatcctttct cgtacacgtc gaccgttttc
    13021 ggcctatatt taaactccaa tatatgtccg ctcttatctg taaggtcgac tgccctgaaa
    13081 tattcttttc tcaaatacac tactaattct tccatttact ctctctttct tcgtgccacc
    13141 tgaagctgca cattcactcc gagaggattc agcacaacac cttttacatc ttccatcagc
    13201 aattctgcga tcttttcccc cggaacaact gccgatctga ccggtcttcc tttcgtgaac
    13261 ttctgaaatt ccagcacatc cgtaaaaaca ggctgataca gcgaaccgtc tttttgcttc
    13321 agtatcggaa tctgaccgtt ctcttcaaca ggaatcaaaa acgttccctt tccgtaatga
    13381 gccaaaagtt cttcctgaag ttcttttaat tcttccgtca tctgcggctg ctcctgtttt
    13441 ctaagttcct gcataaaata agccgccgtc agatggagcg ccgggttttc cacgatctgc
    13501 tttccttccg gaagttcctt cggtatattt ctcgttacca gatctgaaag ctgaactgta
    13561 atttccatat ctgttccgct gttgaccgcc agtgcgttta ctcccattat ataaagactt
    13621 gtgaaaaaag gaagcagctg ctctttcgca agctttgcca cagcgcttac atatttttca
    13681 tcaagaagct tacgcgcctt gtcttttgca tcttccatct gataataaag gaagatctca
    13741 tcgtcaaatg tctcctcgtc acattttaca aacggcatct ttgtcgctgc cgacataata
    13801 acatataatt cacccggatt gcgcagagcc gccaatgtct cctgttttgt cacttttgtt
    13861 tctttctcgc tcattttcag cttttccttt cctgtcttct ctcacctttg tctgtcctga
    13921 tgcattttga gtgtctgtca ataataatac actatctctt tgattttatc aattctaaca
    13981 aatatggaac aaaaataatg catccgatga tcgctgcggc gatcgccgat acaagaaccg
    14041 cccctgccgc tgtatctttc gcgatcttcg ccaatggttt tttctcctta gtcacaagat
    14101 ctaccaccgc ttccaacgcc gtattcacaa gctccagcga caggatcaga ccaaacaaca
    14161 gcaaacagat gcaccactcg acaggtttaa tatgaagaaa agttccgaca gcggttaccg
    14221 caatcactgc cagacagtgg atcttcatat ttctttcttt acggattccg gtccggattc
    14281 cttcaaaagc atacccaaaa cttttattca gaggattttt tctgttgttt tcgtttctgt
    14341 ttttttccat tactttgtac ctgattttat tttctcgaaa aaatatgttt gataaatacg
    14401 tatagtacga tcaaaaacgc aagaatatat ccgatcgccc caagcgccgg aatgccgagt
    14461 attttcggac tcatatctgt cgtacagata atacttgaac tgatcagaag cgccatgacc
    14521 cacaaaccca taacaatgtt tcttacaagt ctgcgcagca gaaaggcaag atcatccgat
    14581 gcccgcagat caagattcac ctttgcctgt ccttttaaat atcccgccat catatcagac
    14641 accatcgacg ggatatccaa cgcctttcgc atcgatttca ctgcgctttt tccgctgctt
    14701 ttcaattctt ttttccagtc aaaattacgg aaaaaatctc ccgaaatacg ggcggcggca
    14761 atttccgcca tattaatatc aggcgcgatc gctgccagaa ctccttccat atgggtcagg
    14821 cctctcgcca aaagcgtcag cccgtgaggc attctgatct tattgacttt catcacatcc
    14881 atcagatcaa ccgtaacttc cccgacattg atcttgccca tatcttcatg accgtacttc
    14941 aacatcagtc cttcgatacc ttcgtataat tttctctgat ccggacgctc tttaaattct
    15001 cccagaccga gcactgcctg ccggatcaaa ccgatatcgt ttaaagcgat cccttcgatt
    15061 gccttgccaa gcatctctct gtcacgctcc gtaagccgcc ccatcattcc catatcgatc
    15121 catacgatct tgccgtctcg tatctttaca tttcccgaat gaggatctgc atgaaaaaat
    15181 ccgtcgtcca tcacctgttt aatgtagtta tcgacaagtt tgctgccgat ctccttaagg
    15241 tcatatccct cttccttcag ccgcttctta tcgtctaccg cacatccatc gatgtattcc
    15301 ataacaagta catggacagt cgtatactgc tgatataatt tcggtgttcc gacaaaatga
    15361 acgtctttgt ttctccttgc gaattcttcc atattagatg cttctgtaag aaagttcatc
    15421 tcgtctctcg tcacagtcca caattcgtct aaaacgagat caaaatcagc catatttttc
    15481 aaactgacag gcggcattaa tttgatcgct ttgcgaagaa gctcaatatc ccttgccatc
    15541 ttttcataaa ttcccctgcg ctggactttt acgacaactt cttctccgct ttttaacgtg
    15601 gcgcgatgca cctgcgcgat cgacgccgcc ccctgacagg attcatctat atgggagaaa
    15661 actcttctcc acgaccgccc gtaagattct tctatgaccg ataaaacttc ctcaaacggc
    15721 atcggtttta catctgaaca aagctgcatc agctcatcgc aataacgctt cggtaaaata
    15781 tcagaatgca tcgccatgat ctgaccgagt ttaataaatg tcggtccgag atcttctatg
    15841 atttcccgaa gctttaaagg cgtcactccc tttgtaatac tatgtttgcg gagcacagcc
    15901 acaatttcct tcagccttga attatattcc atcggttcta tactgctcat aattatgctt
    15961 catccggcgc agtttcgctc gcttcttctt ttttcaggat ctgctctttc aaagcggcaa
    16021 tctgctccgg cgtcatctta tcaagaagat ccgaaagttc ctctgccgat gccggtttca
    16081 gcgcattcac cttctctttt actgtctttt tcacattgtg ctttaactct tcattcagca
    16141 cttttccctg ctctactgtc agttctccct tttctaccat ttcgtcaaga agatcttttg
    16201 acttttcggc agtaacggca actgctccga tccctgccag aaagatcttt ttcaaattat
    16261 cactgagttt atccataata gccttcagct cctttcagcc ggcctacatt tcgtacgcct
    16321 ctgtgctttg atactacgtt tattgttctg ccttactcta tctttttatt cttcttatta
    16381 ttcttattat tctgttgttt tatcagactc gcccgaaccg tatttctctt tatagcggtc
    16441 gatacattct tcgatgatct ttactgcgcc aagcgcgcct tcaaactcgt ccaccgccgt
    16501 tctcttacct tccagatctt tgtatacggt aaagaaatgt ttgatctctt caaaaatatg
    16561 ttttggaagc tcggaaatat ccgtatacgc ctcatatgta ggatctgccc acggaatcgc
    16621 tatgatcttc tcatctcccg ctcctccgtc tgtcatcttc ataacgccga tcggataaca
    16681 tcttacaaga gtcaaaggct caagcggctc tgaacacatc acgagcacat cgagcggatc
    16741 gccgtcgtct ccgagggttc tcgggataaa tccgtaattc atcggataat gcgttgacgt
    16801 atacaagatt cggtcaagga tgatataccc tgtttcttta tctaattcgt attttttctt
    16861 gcttcctttt gaaatctcga tcaccgagat aaaatctgtc ggaaaaatcc tttcctcact
    16921 gatatcatgc catatacttc ccatactaac ctcctcacta ttcccgcata tcagacgcgc
    16981 cgtgttctta cagatcctgt ccggctattt gctctgcgga aacgtcctgt ttaaattcat
    17041 aatatgattt tacctctttc cgttgattcc gtccagtatt atatctccgt cttgatagcg
    17101 aatctgaaac aattcttctt tttcccctat ctcttccctt cttaaataaa catgaaagcg
    17161 aaccaaatca atatcttctc ctaaatctac cgaaaacttc gttgtgcgaa atgagttttc
    17221 ttcgtatttt tgaacaactt tttccgcaaa tgcttctgta tctttgattt catcacaatt
    17281 cgccgcaacg gtcagatcat acacattatt ttcacatcgg ctcgccacaa catccggttc
    17341 gtttccctcc tgcatctgcg cgtgacgcat cacccatatc acaatacaca aaactgccgc
    17401 cgcgcaaaaa atcaacgccg gaatttttcc tgccgcaccc ctgcatcttc tctgatctct
    17461 ttttcgcttc actttgcatc acctgtcttt ttcgtcaata tttgtatcac ttttacaacc
    17521 gattatataa tctgtttttg ccggaatcaa gaaaatcggt tgaaaatatg agacattttt
    17581 ataacagatg attttcacat ataaaaaaga caccccactt cctgtgagat gtcttctctt
    17641 ctgttctatt ccatttctct gtactgctgt gcgatattca atgcgtgatc gcccattctt
    17701 tcaaaatctg tcagtatttc agaaaagacg ataccgcttt gcggcttaca ttttcctttt
    17761 ttcagacgct gcatctgctt tttaaaatac ttatcccgca tatcgtccgt tttctgctct
    17821 atgataactg cctgacttag tatccgttct acattttcgg aagttacttc tttcagatta
    17881 tcaagcgccg caatacactg cgctttcatc tcattaagtt cttctttaac cgtatccgaa
    17941 aaatcaatct cccattttct catatcgtcg ccatattccg caagattgac agcatgatct
    18001 ccgatccgtt ccagattgct tataatcgca taatatccgt tgatctttct cgaatctgac
    18061 atattcatct cgcttgccat aagtgaaacg atatattcgg atatgccttt attgaggaaa
    18121 tcaatatatt cttctctctc agacactttt ttgcgaagtt tctcatcata ttgaacaagc
    18181 gagtcaaacg agtcgcttat atttttcgcc accatatctc tcattctgtt aacttcatca
    18241 cgaacctgtg acactgccac cgcgctgtgt ccgatcgcat aggaagattc aaacggacgg
    18301 atatatttca ggcgaaggtc ttcatcgtct gcctttttgc tgtccggaag aatgcgaacc
    18361 gctatattcg ccatatgtgt tccaaacgga agaagcagca gtgtcgtcac gatattaaag
    18421 actgtatggg catttgcgat ctgcgccacc ggatctccgg gcgtcatcgc ttcgatccat
    18481 gtcacatacg gcgtcacaag acagatcacc gtaaataata ccgtgccgat tatattaaat
    18541 aacagatgga taaccgtcgt acgctttgca tttaccttca ttccgatcga tgccaaaact
    18601 gccgtgatac atgttccgat gttttgtccg aacaaaacga acaccgcgct tgagagcggg
    18661 atcataccgg tagacgcaag cgcctgcaaa ataccgaccg atgcagaaga tgactggatc
    18721 actgccgtaa aaagcgcgcc taccaaaata ccgagaagcg gattgtcgaa cttcgtcatc
    18781 agactgataa acgcttccga ctctttcaaa ggcgacattg ccgcccccat catatccatt
    18841 cccataaaaa gaataccaag tccggcaaaa atactgctga tatgatgtac cttttcactt
    18901 ttgataaaca tgatcgctcc tacgcctgcg atcgcaaaaa gcggagcgat cgctccgata
    18961 tcaagagcaa tgagctgacc tgtaatcgtc gttccgatat tggctcccat gatcacccat
    19021 accgcctgtt ttaatgtcat caaccctgaa tttacaaacc ctactaccat gaccgtcgtt
    19081 gcagaagacg actgaatgac cgctgtgatg gctgctccga caagcacacc ttttatacgg
    19141 ttcgatgtca gtttttcaag gatcgacttc atccggttgc cggctgctgc ttccaatccc
    19201 gtgctcatca tctgcattcc gtataaaaac aatgcaagac cgccgagcaa agcaagtacg
    19261 tctgttattc ccattccatt tttctccttt tgtaaatcgt cttatttttt ctatattctg
    19321 tttttcttta tacacacgtc tgcttcgacg cgctttttta aattaacatc cgccaaccgg
    19381 tatgtcaatt ccattttacc ctttccctac attttcttaa cactttctta acgatgcctt
    19441 aacagaccgc tcattgccga taatataaag ctgcgcggtg aggacgatcc ggaatcgctt
    19501 caacgcacag ccttgacaaa atgcttttta ggtttttatt ccacactttt cagtgattcg
    19561 accatattga tctttttaag tttaaaatac atcgctccgt ttacgatcat agagaacgca
    19621 accgtcaaaa gaaaactgta tacaaaactg ctcatatcga tatttctgcc aaacataacc
    19681 gtatctatct cgacagtgac aatgataaag cgatgaagta tctttccgag agcgacgccg
    19741 aaaagagcgc ctaaaaccgt aagtacgata ttttcccgat atacatacgc cgaaacttca
    19801 ttcggataaa atcctaaaac tttcagcgtt gccagttctc tctttcgctc tgtaatatta
    19861 atgttgttca ggttatacaa aacgacaaac gccagcattc ctgccgatat tgtcagaaca
    19921 acgatcacaa tgtcaagcgc tcccaacata tcgtctacct gttgtttcaa atcagtcgta
    19981 tagctcacac tcaacgctcc cggaagctga agaatatctt cgccgacact ttgcgcttct
    20041 tttgtcgttc ctgaaaccgt cttatagaaa acggcattgt actccggttt ttcgttataa
    20101 acagcctcat acgcatctgc cgtcatgtac aggtaatgtc ccatataatt ttcgcagaca
    20161 gctttgatct tgaccttgag atctcctttc tcttcatcac ggatcgtaat catgtctcct
    20221 gcctgtactt ccagctcctt cgcgattttt tctgtcaaaa caacttcttt ttcatccaga
    20281 gaatatcttc tatccgtcgt cctttctttc aaagtcacaa actccgggaa ttgcttttta
    20341 tctttcggca catccagata gacatcgtgc catttacttc cgtttccgat tgtaatctga
    20401 gtcagtatgc cttccatcgc atcgtctact tctgtttctt ttccaagcat ctgcgcgact
    20461 tcaccttttt ctttttccga agcgccttcc gccataatga ccgttccgtc atatcgctga
    20521 atctcgccgt actgaatatc gacaaccgca aagatcgaat ctttcaaacc gaaccctacg
    20581 atcatcagtc ccatacatcc tccgatgccg aaaatcgtca taaaaaaccg tttcttatat
    20641 ctgacaagat tacgcacgct tgctttccag gaaaaattca gccgcttcca caaaaatgta
    20701 atcttctcaa aaacgactct ctgaccctgc ttcggcgtcg gcggacgcat cagttgggcc
    20761 gcctgttcgc gcagttcctt ataacatgcc agtatcgtcg ccgccaatgt acaaaacagc
    20821 gccgttcccg ccgctcctat cccgtacatc acattatacg gtatgagcag ttcattcatg
    20881 tgtttataca ttatcccgta tgcattgatg ataatatacg gaaatatctt ctccccggta
    20941 aaaataccgg ccgcgcttcc aagaagggtc gccagaaacg catatcccaa atatttccct
    21001 gctatcgaat gtctcgcgta tccaagcgct tttaatgttc cgatctgagt cctctgctct
    21061 tctaccatac gggtcatcgt cgtaagactg atcagtgccg ccactaggaa aaatatcacc
    21121 gggaacactt cgccgatcgc tttcattcgg tccgcattat caccgtatcc cgaataatcc
    21181 ggaaggtcat ttcggtcata aatgtaccat ttcggctttt ccagttctgc aacttccgct
    21241 ttcgcatcag ccagctctgc ctgcgcttcc gctatctttt gctcgccttc ggcgatctcg
    21301 gcgtttgcct ctgcttcacc tgcataatac tcttcccatc cggaagcaat ctcctgctcg
    21361 ccggatatga tcccggcttc cgcgcctgcg atctgctgcc ttgccgcctc aagctgtgca
    21421 tacccgttat caatctgccc ttgcgcatat tgaagctgag ctctcgtctg atcgatctgc
    21481 gcctgtgcct cgaccacagc tgcgtctccg atctggatct gcatgctcat ctgctccaac
    21541 gccgcaagcg tttcttcgtc tgtcattccg ctttctttca aagcctcata ctgcgctttc
    21601 accgcattta attgttcaat ctgctgattc aactgcacaa ttccctgatc aagcgccgct
    21661 gttccgttct gaacttctgt ctgcttctgt gccaaggtct gctctgttgc ggcaacctcc
    21721 gctttggagt tctccaactg cactttccca ctatcgatct gtgcctgcgc gttttccagt
    21781 ttctgccttg catccgccag ctttgccgcc gcttccgctt tcccgttttc cagcttcgtc
    21841 tgtgcatctg ttacttcggc ttccgcctcc gcaattttcc cgttcgcctc atcgacaatt
    21901 tccgtatacc gggctttttg tctctgctcc ttgatcttct caatattttc ttttgctttc
    21961 gcgaccgtat cttcatattc cgaagtaaat gccgtcaatt cttttgctcc tgccgcctga
    22021 atacaaatct ctgtatagac gtccattgca aagctttctt cctgtacata tacaaacgcc
    22081 cgcacacttc cgtttccgat tgtcgtactg ccccgctgaa aagcaatata ctccggactg
    22141 ctcactgtac ctgtgatccg aaacgtatcc gttttcaaag aatctgtaac ctcggcatcc
    22201 gtaccgcttg aaaacgtgat cgtatctccg atcttcagtt tgcttttttc catgtaatcc
    22261 acatcaacga cacattcatt ttccttattc ggaagcctgc cgtcctcaag ctgtatctca
    22321 ttcatcgtcg gaagcataga catgacatga acaacgattt gattatcacc gtctgccaaa
    22381 agagcgtcca cagaataccg gccttccaca tgcccgatcc cttctgcttt tgaagccgcc
    22441 tttacgtcat cttccgtcaa tcccatcgta ctgatgatcc taagatccat catattctta
    22501 ttatcaaaat acgcgtctcc cgaataacgc atatccggct ccgaggcacg tattcccgaa
    22561 aagaaggcac atccgatcgc aacgatgaaa aagatggaaa ggaaacgccc gaggctgcgt
    22621 ctgatctcca tataaaaatc ttttctcagc gcacgttttt tcattcacac cgcctgccat
    22681 ttccatttct tatttttatc cgaactacca ctcgatctca tcgatcgaca tcgggttttc
    22741 atttgtttcc atatgagaca cctgcccgtt cttaatatga atcaatctgt ctgccatagg
    22801 tgcaagagcc tgattatggg taatcacgat cactgtcatc ccccgttccc ggcacatgtt
    22861 ttgtaaaagc ttcaaaattg cctttcccgt attataatca agcgctcctg tcggctcgtc
    22921 gcaaaggagc aacttcgggt tcttcgcaag agcgcgggca atcgaaacac gctgctgctc
    22981 gcctccggaa agctgcgccg gaaaattatc caatctgtcg ccaagcccta catcttccaa
    23041 tacctttttt gcatcaagag gatctttaca tatctgaagc gcaagctcta cattttcaag
    23101 cgcggtcaga ttcgggatca gattgtaaaa ctgaaatacg aatccgatat catccctgcg
    23161 gtatccggtc aactgtcggg aatcatacgc cgtaatatct tttccgtcca ctgtcagcgt
    23221 tccgcctgtc gcggtatcca tgccgcctaa aatattaagc accgtcgttt ttcccgcgcc
    23281 gcttggtccg acaattacaa caaactctcc ttttttgata gagaaattaa tattgtctgc
    23341 tgcccggatc tccacttctc ccatatgata aactttcgtg atatccttta atttgacaaa
    23401 ttcctccatc ctgtcacctc tttcatccca tatgttttcc tcattcgatt ttcatcgcac
    23461 gctatttaat tttgccgaat atctaaataa tacaagccca actaaaaaca tcacggaaag
    23521 tgcgctgaca gcaatattga tgctccccgt catctgactg ataaaagata c
//
LOCUS       DBMDXF010000004        22385 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_746_length_22385_cov_2.393421, whole genome shotgun sequence.
ACCESSION   DBMDXF010000004 DBMDXF010000000
VERSION     DBMDXF010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22385)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_22385_cov_2.393421"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="ACZPD4_00355"
     CDS             <1..250
                     /locus_tag="ACZPD4_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377562.1"
                     /translation="GSVFISILGIVFGTLSENQIMQEMAESGIRFDEYAVIFILIIGL
                     ATILWFISTIQYIVGNKYKSLLYMCESILEAKENTMIE"
     gene            980..2131
                     /locus_tag="ACZPD4_00360"
     CDS             980..2131
                     /locus_tag="ACZPD4_00360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1377563.1"
                     /translation="MEIVAAIDSFKGSMTSVQAGKAAAKGIHKVDPEAKVHVRPLADG
                     GEGTVEALVSGMNGTIRKVSVTGPIGKTVVCEYGVIENTGGKTEEKTAVIEIAKAAGL
                     TLLSEEERNPLYTTTYGVGEVIKDAVRNGCRKFIVGIGGSATNDGGAGMLQALGFGLL
                     KENGEQIPIGARGLEELAEITDDNVIPELAECKFKIACDVTNVLCGETGASAVYGPQK
                     GADEEMTERLDRLLFSYASLVKKKIPKADSMYPGTGAAGGLGFAFLTFMDAQLESGIQ
                     IVIKETGLEQEIAKADLVITGEGRMDGQTAMGKAPIGIAKIAKKYGKPVIAFAGAAAR
                     DAGACNEQGIDAFFPILREAVSLEAAMKRENAEANMEAAVEQAYRLCRL"
     gene            2320..2892
                     /gene="yfcE"
                     /locus_tag="ACZPD4_00365"
     CDS             2320..2892
                     /gene="yfcE"
                     /locus_tag="ACZPD4_00365"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR1377564.1"
                     /translation="MKWMIASDIHGSAVYCGQMLEAYRREKADRLLLLGDLLYHGPRN
                     DLPKGYAPKEVIRMLNEVKEELLCVRGNCEADVDQMVLEFPVMADYCVLQLEDTGVVF
                     ASHGHVHNPQCMPPLKNGDILLNGHTHVPACEKIADENGNEYMYLNPGSVSIPKEGSS
                     HSYMVYEKGTFTWKNMSGEVYREWKKDSRF"
     gene            2868..3839
                     /locus_tag="ACZPD4_00370"
     CDS             2868..3839
                     /locus_tag="ACZPD4_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1377565.1"
                     /translation="MEERFALLIDADNVSAKYIKPILDELSKYGNVTYKRIYGDWTRP
                     NSAGWKEELLQNSITPIQQFSYTYGKNATDSAMIIDAMDMLYASELEGFCLVSSDSDF
                     TRLASRLRESGKRVIGMGEAKTPLPFRKACEIFTELELLLEDNMMGVSEREEKTVFHT
                     VSGKESKKMISATKEQVEEAVVKIITENKNNDRETGLGEVGSRLVKLYPDFDVRRYGY
                     SLLSKFLETLPRLMLIQEGTKVSVALNEDKSRKEMVEEYILYQIEASGESGIPLSALG
                     NRIRLKFADFKVRDYGYSQFKQYIQSFPEVELQGEGERTKAVYAPVL"
     gene            3940..5478
                     /gene="asnB"
                     /locus_tag="ACZPD4_00375"
     CDS             3940..5478
                     /gene="asnB"
                     /locus_tag="ACZPD4_00375"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1377566.1"
                     /translation="MIKVKEYRGHIRNWEELCERLDIPLDLTREEREEQILVKAYETW
                     GNEMADHMHGMFAFALWDEAEEKLFCLRDQFGTKPFYYYETADGKLLYGTTIRKIMEQ
                     PGFVKELNEEMLQLYLSLTYVAGENTFFRGLKKLLPGRYLVWQNGKLTIERYWMPQFH
                     PDNSRTLEQWADEIHTTIGEIMPEVKTKEEYAESFLSGGVDSSYVLAMSDVETTDSCG
                     YDEERFDESGLAKQTADILGRKNLRCRITPEEYFAVVPYVMYNMEQPLGDASAIAFAI
                     ACRETAKHTKICYSGEGADEFFGGYNMYRNAERYGENLKTFYVGNTNIMKEDEKKRIL
                     KKYDPDVLPIELARGIYEETEGLDPLTKMSDVDIQIWLEGDIYLNVDKMSLAAGLEIR
                     MPLTDRRIFDIASRMPSEYKVNEEQNKVALRTAAAKVLPEEIAFRKKLGFIVPIRIWM
                     ADDRYNADVRRLFASDIAEKFFHVEEIRAIFDEYVGGNSDNWRKVWTIYTFLVWYEEY
                     FVKR"
     gene            5576..6838
                     /locus_tag="ACZPD4_00380"
     CDS             5576..6838
                     /locus_tag="ACZPD4_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1377567.1"
                     /translation="MTTKKSVKKMYCRMFQSVMKAGISTIPWRTPKLMKGAGAVRHLP
                     AAIKRRGLKKALIVTGPTLIGRGLLDGVLEEMEEQGLLYVIYNKVEQNPTDENVEDAV
                     KVFLENGCDCMLALGGGAPLDCAKAVGARIARPNRQIRQLQGLFRVVKQIPMLFAVPT
                     TAGSGSETTISAVITDTATHHKAAINDLRLMPKYAVLDPELTLGLPPDVTAMTGMDAL
                     CHAVEAYTNDKYNSDTERELCRKAVRLIYENLLKVYENGSDIEARQKMQQAAFYAGRA
                     FTRGGVGYVHAIGHAVGGLYGTPHGLAMAIFLPYVMRAYGDAACKKLAELSDVCRLTT
                     PQESDSVKADAFIGWIEELKVKMQIPQYPEMIRKTDVKQIAKWAEKEANPLYPTPEVW
                     NRREFERFLLNVCVQGSSEKDRQCCQDK"
     gene            6860..7621
                     /locus_tag="ACZPD4_00385"
     CDS             6860..7621
                     /locus_tag="ACZPD4_00385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1377568.1"
                     /translation="MIIDTHAHYDDEQFDEDREEILGKMQDAGIGMIMDAGSTILSWD
                     KIVKLTEEYPFVYGAIGVHPDEVGNLDETQFARMERLLDKEKIKAVGEIGLDYYWDKE
                     NHDLQKEWFIRQLDLARKKEKPVIIHSREAAADTMEIMKEYASGLRGVIHCYSYSAEM
                     AKEYVKMGYYIGIGGVVTFKNAKKLKQVVQEIPLESIVLETDCPYLAPVPYRGKRNSS
                     LYLPYVAEEIAALKGAAAEDVVLQTEENARKLYGL"
     gene            7722..8600
                     /gene="rsmA"
                     /locus_tag="ACZPD4_00390"
     CDS             7722..8600
                     /gene="rsmA"
                     /locus_tag="ACZPD4_00390"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1377569.1"
                     /translation="MKEPTLGNPQNTIAVLQKYNFIFQKKFGQNFLIDTHVLDKIIRA
                     AEIGKDDLVLEIGPGIGTMTQYLSCAAGKVIAVEIDRALIPILEDTLDGYDNVRVINE
                     DVLKVDIRKLVEEENEGRPIKVVANLPYYITTPIIMGLFENHVPIKSITVMVQKEVAD
                     RMQVGPGTKDYGALSLAVQYYAKPYIVANVPPNCFMPRPKVGSAVIRLERHEHPPVEV
                     RDEKLMFRVIRASFNQRRKTLANGLKNSPEIDFSKEEIEGAIEKLGKGASVRGEALTL
                     AEFAQLANYFCDLREK"
     gene            9028..10398
                     /locus_tag="ACZPD4_00395"
     CDS             9028..10398
                     /locus_tag="ACZPD4_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR1377570.1"
                     /translation="MAKNIDSIFFDITPKIEELALKCEQNNAIEKELYTKYEVKRGLR
                     DLNGKGVLAGLTNISDVCASKIVDGKSVPCEGNLYYRGYNIKDLVRGFLEADHPGFEE
                     TAYLLLFGELPNKAQLEEFQNMIAERRMLPPNFVRDVIMKAPSRDMMNSITRSILQLY
                     SYDEKADDTSIPNVLRQCLNLISQFPMLMVYGYHAYNYRRGEDLYIYAPKAELSTAEN
                     ILMMLREDRKYTKLEAEILDMALVLHMDHGGGNNSTFTTHVVTSSGTDTYSTIAAAMA
                     SLKGPKHGGANIKVTQMFEDMKSVLTDWEDEDQIRAYLEALLDKKAFDRKGLIYGMGH
                     AVYSISDPRADIFKGFVKKLAKEKGHEAEYGLYEKVEHLAPEIIGEKRKMYKGVNANV
                     DFYSGLVYSMLDLPPALYTPIFAAARIVGWSAHRLEELKNVDKIIRPAYKPLAPYRDY
                     IKLDDR"
     gene            10414..11355
                     /locus_tag="ACZPD4_00400"
     CDS             10414..11355
                     /locus_tag="ACZPD4_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1377571.1"
                     /translation="MRDEFYFPSKDGNTEIHTIEWKPEGEVKAVLQICHGMVEYIKRY
                     DEFAEFLCGKGFYVVGNDHLGHGKSVQAKSEYGFFSEKYGNACVIGDIHTLRQRTMKK
                     YPNVPYFMLGHSMGSSLLRQYIQMYGKGLSGAIIMGAVADHKKATLVFGKRLCRLMAA
                     VRGWHYRSHFVDHLVVGNFNRKFKPARTRADWVTSDREHLDAYVSDPLCSFVFTVNAY
                     YSMFSGMISMQRKEGVYMIPKNLPILFAAGSEDPVGNFGKGVRKIYEKYRAAGIRDVS
                     LQLYTGDRHEILNESDREQVYDDLFEWLAGHMENKVL"
     gene            11413..12804
                     /gene="asnS"
                     /locus_tag="ACZPD4_00405"
     CDS             11413..12804
                     /gene="asnS"
                     /locus_tag="ACZPD4_00405"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1377572.1"
                     /translation="MELTTIRELFKDRESYLGKKVTIGGWVRSLRDSKTFGFIVVNDG
                     SYFEPLQIVYHDEMENFAEVSKLNVGAAIIVKGTLVATPQAKQPFEIQADEVQIEGAS
                     ASDYPLQKKRHTFEYLRTISHLRPRTNTFQAVFRVRSLAAYAIHKFFQERGFVYVHTP
                     LITGSDCEGAGEMFRVTTLDMDQIPKNEDGTVDYTKDFFGKETSLTVSGQLNGETYAQ
                     AFRSIYTFGPTFRAENSNTTRHAAEFWMIEPEIAFADLEDDMDLAESMLKYVINYVLE
                     NAPEEMNFFNSFVDKGLLERLNNVANSDFARVTYTEAIEILEKNNDKFEYKVSWGCDL
                     QTEHERYLTEQVYKRPVFVTDYPKEIKAFYMKQNDDGKTVAAVDCLVPGIGEIIGGSQ
                     REDDYEKLLARMNELGLDEKDYGFYLDLRKYGSTRHAGFGLGFERCVMYLTGMSNIRD
                     VIPFPRTVNNCEL"
     gene            12992..14044
                     /locus_tag="ACZPD4_00410"
     CDS             12992..14044
                     /locus_tag="ACZPD4_00410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1377573.1"
                     /translation="MKFIHIADVHLGAQPEAAVYSQSRGRELWESFEKILGVCEDERT
                     DLLLIAGDLFHRQPLVRELKEVNYLFSELTATKVVLIAGNHDHLQKDSNYRSFEWNDN
                     VYPLFGKKLEYVDFPELETAVYGLSYYEREICQPLYDDVAAAGIEKNEILLAHGGDDR
                     HIPFDKKKLSRSGFSYIALGHIHKPQALQKDKMIYAGALEPIDQNDVGQHGYVKGELK
                     DGKAAIQWIPFAGREYIHSSVEVERSDTEGSIRKRVKQLINEYGNENIYKITLAGKRD
                     PDIAFEVNHLAEEGCVLEILDETIPAYDFEKLYAENKETLLGRYIEKFAGCEEGSVEY
                     CALCEGVEALLTGNRG"
     gene            14050..15639
                     /locus_tag="ACZPD4_00415"
     CDS             14050..15639
                     /locus_tag="ACZPD4_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1377574.1"
                     /translation="MVIRELYVKNFGKLSEKHFYFRDGVQVISGENEFGKTTLHAFVK
                     AMLFGLERGRGRAAAKDDFTKYEPRSGGRYAGVMRFDCGGRHFRLERTFGTGVKNSKS
                     AALICEDDGEELSVEHGDLEMLLGGLTAELFDSTVSVGQLKSRPGEALSDALENYAAN
                     YYETGGTELDLSGAVQILREKRKKTDRFIREEADKEERKIQKMLQECEYLEQDMDEIQ
                     REYEERKQEWELLEKTIKNQEKEKESDFNKRSDEGTKESRSKYFIAAGLGGLFTGAAG
                     LLWSRFMAEQSAVPSAPFAWIGVFAFVIGVCALAAGGYEAGKKSGKRKKEDARDVPYN
                     NSDKDDERKKKQKQQLEWQMEHLRLEWKEKELRCQNVREQCGDVKESDAGKRLREQRE
                     ALVMAEEILVRTAKETSDVISHKINARASEIFSEITDGKYRSVNIQKGAGISAWNGMD
                     RISVDRLSEGTLEQIYFSIRMAASEMLLEEPMPVILDDAFAFYDDKRLESVIKWLSRQ
                     KKQVIILSCHSREAKLLEHLV"
     gene            15711..17060
                     /locus_tag="ACZPD4_00420"
     CDS             15711..17060
                     /locus_tag="ACZPD4_00420"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1377575.1"
                     /translation="MKIALPRKVMMIIKNLQLHGYEAYAVGGCVRDSILARRPKDWDI
                     TTSARPEEVKKLFRRTVDTGIEHGTVTVLLGRDGYEVTTYRIDGAYEDSRHPKEVLFT
                     NRLEEDLRRRDFTINAMAYNDEVRLVDVFGGMQDLNHHLIRCVGDPRERFSEDALRIL
                     RAVRFSAQLNFPIEEETAEAVRELAPTLENISAERIQAELVKLLVSAHPERIQDAYEL
                     GITRIILPEWDAMAGVEQNTPHHKYDVAQHTLHALKYVKRDKILRLTMLFHDMGKPAR
                     KTTDENGRDHFKGHALVSEAIARDVMQRLKFDNDTIKKVTRLVCFHDYRTEATPANVR
                     RAMNRIGVDLFPYYLAVRMADAKAQSSYRRREKIENIVAMRKFYQEAIIKDECVTLRQ
                     LAVSGRDLMNLGMRPGVEIGRMLSELLEYVIDNPEKNDKKILCNYVKKKISQRNKTV"
     gene            17145..18236
                     /locus_tag="ACZPD4_00425"
     CDS             17145..18236
                     /locus_tag="ACZPD4_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HIR1377576.1"
                     /translation="MREEYELEVLEKYDIEVKSTRRIRGAFFCIGSELKSGRAGDFGD
                     CLQSRSVPFGAAGDYDTKERPMLLKETKLSERRALFLYDALSEIERCGNVKVDTPVFT
                     KEGELRAASREGAFYMLKKWYQGRECDVRQEQDVVRAAAKLAVLHKDMEKLAVKIRPG
                     ADVQGKNPVEEVRRYNRELKKIRSFIRSRPVKNDFEILFLENFEKMYRTADDILARME
                     TSGCRKLFEESVSKGSVVHGDYNYHNLIMLRDDIAVTDFEHMHTDIQIKDFCYFLRKA
                     MEKNQWKQKTGQKILEAYEEVRPLSEREKEFAALSLAYPGKFKKIAGSYYRSNKAHLS
                     EKNVEKLQICIRQTEEKYEFLSRIFPLNL"
     gene            18313..20052
                     /locus_tag="ACZPD4_00430"
     CDS             18313..20052
                     /locus_tag="ACZPD4_00430"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1377577.1"
                     /translation="MDYKARYEEWISNSYFDEDTRAELESIKEDENEIKERFYMDLEF
                     GTAGLRGIIGAGTNRMNIYTVRKATQGLANYIMKNDGQGKGVAIAYDSRHMSTEFAKE
                     AACCLAANGIKAYIFESLRPTPELSYAVRKLGCIAGINITASHNPPEYNGYKVYWEDG
                     AQITPPHDKGIMDEVKAVEDYTTMKTMTEEDAKAAGLYEVIGAEVDDAYIAELKKQVL
                     HKDAIEAAGKDLKIVYTPLHGTGNIPARRILKELGFENVYVVKEQELPDGDFPTVSYP
                     NPEAEEAFELGLKLAKEIDADIVLATDPDADRLGVRVKDKDGVYHDLTGNMSGCLLAD
                     YEIGQRKELKGLPEDGYLIKTIVTSNMADAIAEHYNVGLIECLTGFKYIGQQILGFET
                     KGKGEYLFGFEESYGCLIGTHARDKDAIVATMALCEAAAYYRTKGMTLWDAMIEMYER
                     YGYYKDDIQSITMKGIEGLEKIRQILATLRSEPPKEIAGYKVLKVRDYQADTIRDMET
                     GEVTATGLPNSNVLYYELPDGAWVCVRPSGTEPKVKFYYGVKGTSLTDADVKSDEMGK
                     AIMEMVKNLDKAV"
     gene            20158..20430
                     /locus_tag="ACZPD4_00435"
     CDS             20158..20430
                     /locus_tag="ACZPD4_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1377578.1"
                     /translation="MNKTELITAIAESADISKKDSEKALKAFVDVVTEELKKGEKVQL
                     VGFGTFEVSERAAREGRNPQTGKTMKIEACKAPKFKAGKALKDAIN"
     gene            20548..20787
                     /locus_tag="ACZPD4_00440"
     CDS             20548..20787
                     /locus_tag="ACZPD4_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1377579.1"
                     /translation="MRLDKFLKVSRLIKRRTVANEACDAGRVSVNGKPAKASVQVKPG
                     DVIEIYFGTREVKAEVLKLEDTTKKEKAEELFRYL"
     gene            20875..21159
                     /gene="yabP"
                     /locus_tag="ACZPD4_00445"
     CDS             20875..21159
                     /gene="yabP"
                     /locus_tag="ACZPD4_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HIR1377580.1"
                     /translation="MEEQRIAKAHKLVVNNRKTSTVTGVLDVLSFDLNEILLETEQGM
                     LMVKGTDLHVNRLTVEKGEVDLSGNIDSIAYSSVTPQAKQGDSFLTKLFK"
     gene            21163..21522
                     /gene="yabQ"
                     /locus_tag="ACZPD4_00450"
     CDS             21163..21522
                     /gene="yabQ"
                     /locus_tag="ACZPD4_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR1377581.1"
                     /translation="MIPGIGAEAGIFLYAALAGVEAAAAYHVLVCFRKLVPHSGWAVS
                     LEDFIYWLAASIYIFRKMYDTTYGSIRWFFVFGMLCGAAAGHAGIRKIVKLCAERCGK
                     KEKTEKSCRKARKENRK"
     gene            21603..21953
                     /locus_tag="ACZPD4_00455"
     CDS             21603..21953
                     /locus_tag="ACZPD4_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1377582.1"
                     /translation="MNGKKKRTADRRNAGRRRSAHLHSYKKSVLMICTVLVFLSGVLA
                     VGAVKLYAKNNAYKAQEEELAAQIKEEEKRSEEVKEYEKYVQTDEYIKETAEDKLGLV
                     NPNEIIFKPSNKNK"
     gene            22190..>22385
                     /locus_tag="ACZPD4_00460"
     CDS             22190..>22385
                     /locus_tag="ACZPD4_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377583.1"
                     /translation="MKEGQALQGSPYVEQIEKYASSLKQLSHTFLELEAHKEEFSGAD
                     IDGMFDREEKFRPEPEQYTEL"
BASE COUNT         6947 a         3782 c         6187 g         5469 t
ORIGIN      
        1 tggctctgta tttatttcaa tattaggaat agtatttggc acattatcgg aaaatcaaat
       61 catgcaggag atggcagaaa gtgggattcg ttttgatgaa tatgcagtga tatttatctt
      121 aataattggt ttggctacta ttttatggtt tatatcaaca atacaataca ttgttggcaa
      181 caaatataaa tcgcttttat atatgtgtga aagtatttta gaagccaaag aaaatacaat
      241 gatagaatag tataaataac gataaaaaga acatctatca gaaattgtac cgcccccaaa
      301 aaaacttaga cctaaaagga cgttattatg aagtccttgc ccatcaactt tccgaggtga
      361 atcttttcgt tagtgccatc aatcccaaac tttggaatgt tcattctggc attgctgaca
      421 ttcgtattta cgtttatcag atagtgcttt aaggcataga aaaccaccct ctaaactttg
      481 accggtttca gggtggtgat tctattacaa tcttgtcgag gtcaaccccc ttgtgggcgg
      541 ttgttccttt tatgtcttta atatagtata tccatcagag cgatacaagg gctattcaga
      601 ttttactata gaaagtcggt acgttttgtg gtgatcttca gaagggagat tcttaaagac
      661 ctcttcgtct ataatcagat ttgtccaatc attcgtcaat atttccccat caaatagagt
      721 tactcttatg aaatctggta atttataaat atcgtcagta gtatactgtc gttttggagt
      781 gggaatagta acacatcctt tcatggtgat agtgttgccg gatcattctt taggaatcgg
      841 tcgtgcaaag ttattgaaat gtaaaaaaag aaatgatacg ctgtgtacat tgaaggtatt
      901 tatatgatga caaacaagtg cggggaaaca tttttataat gttttctgtc tgagtaaagt
      961 gaaaggaatg gatggattta tggaaattgt ggcggcgatt gattcgttta aagggagtat
     1021 gacttcggta caggcgggaa aagccgccgc aaagggaatt cataaagttg atcctgaagc
     1081 aaaagtgcat gtcagaccgc tggcagacgg aggcgagggg acagtggaag cgttggtgtc
     1141 cggtatgaac ggaacgatac ggaaagtttc tgtaaccgga ccgatcggaa aaacggttgt
     1201 gtgcgagtat ggtgtgatcg agaatacagg tggaaaaaca gaagagaaaa cagcggtcat
     1261 tgagattgcg aaagcggcag ggctgactct tctttcagag gaagaacgaa atccgttgta
     1321 tacgacaact tacggtgtgg gagaagtgat aaaagacgct gtgcgaaacg gatgcaggaa
     1381 atttattgtc gggatcggag gaagcgcgac aaacgacggg ggagcaggaa tgcttcaggc
     1441 tttgggtttt ggcctgctga aggaaaacgg ggaacagatt ccgattgggg caaggggtct
     1501 ggaagaactg gcggagatta cagatgataa cgtgatccct gaacttgcgg aatgtaaatt
     1561 caagattgcc tgtgacgtaa cgaacgtatt gtgcggagag acaggagcaa gcgctgtgta
     1621 cggtccgcag aaaggcgcgg atgaagagat gaccgaaagg ttagatcgtc ttctcttttc
     1681 ttatgcgtct ttggtgaaga aaaagattcc gaaagcagat tctatgtatc cgggtacggg
     1741 ggcggcaggc ggtttgggat ttgcatttct tacatttatg gatgcacagt tggaatccgg
     1801 tatccagatt gtaataaaag aaacaggctt ggagcaggaa attgcaaagg ctgatcttgt
     1861 gattaccggg gaagggcgta tggacggaca gaccgctatg ggaaaagcgc cgatcgggat
     1921 tgcaaagatt gcgaagaaat atggaaaacc ggtcattgca tttgccgggg ctgctgcgcg
     1981 tgacgccgga gcatgcaatg agcaggggat cgatgcattc tttccgatat taagagaggc
     2041 ggtttctctt gaagcggcaa tgaagcggga aaatgcggag gcaaatatgg aagcggcagt
     2101 tgagcaggca taccgtttgt gtagattatg accggctgtg cataaaagaa aatttcgtaa
     2161 aatgctcggg tcttttgcgg tgtttgtgat atagcagcct gtcaggaggg cagatttaca
     2221 aaaatagaaa aattatgtta aaatgataaa aataaaggga cagggacaat gttttatgta
     2281 gtttgaaaac tgtcaaatct aaatatgtgg agaaaaagta tgaagtggat gatcgcgtca
     2341 gatatacatg gttcggctgt gtattgtgga cagatgcttg aggcgtatcg aagagaaaaa
     2401 gcggacagac tccttctttt aggagatctt ttatatcatg ggccccgcaa tgatctgccg
     2461 aaaggctacg ccccgaaaga agtgatacgg atgttgaatg aagtcaagga ggaactgctc
     2521 tgtgtgaggg gaaactgtga agccgatgta gaccagatgg ttttagagtt tccggtcatg
     2581 gcagattatt gtgtactgca gcttgaggat acaggcgtgg tttttgcatc ccacggacat
     2641 gtgcacaatc ctcagtgtat gcctccgctg aagaacggag acattttgct gaacgggcat
     2701 acgcatgtgc ctgcctgtga gaaaatagcg gatgagaacg gaaatgagta tatgtatctg
     2761 aacccgggat cggtttcgat tccgaaagaa ggttcttccc acagctatat ggtttatgaa
     2821 aagggaacat ttacgtggaa aaatatgagt ggggaggtat acagagaatg gaagaaagat
     2881 tcgcgctttt aattgatgcg gataatgtgt ccgcaaaata tattaaaccg atcttagatg
     2941 aactgtcgaa atatggaaat gtaacatata aaaggatcta cggagactgg acccgtccga
     3001 acagcgcggg gtggaaggaa gaacttttgc agaactctat cactccgatc cagcagttta
     3061 gttatactta cggaaaaaat gcaactgact cggcaatgat cattgacgct atggatatgc
     3121 tgtatgcgag cgaactcgag gggttctgtc ttgtttcaag cgacagcgat tttacaaggc
     3181 ttgcaagcag gctgcgggag agcgggaagc gtgtgatcgg aatgggagag gcgaaaacgc
     3241 ccctgccgtt tcgtaaggca tgcgagatct ttacggaact ggaacttctt ttggaagata
     3301 atatgatggg cgtttccgag agggaggaaa aaaccgtttt ccatactgta agtggaaaag
     3361 aatcgaaaaa aatgatttcg gctacgaaag aacaagtgga agaagcggtc gttaagatta
     3421 ttacggagaa taaaaacaat gacagagaga cgggacttgg cgaagtcggg agcaggcttg
     3481 tcaagctata tcctgatttt gatgtccgcc gctatggtta tagtcttttg tccaaatttt
     3541 tggaaacgct tcctcgttta atgctcattc aggagggaac aaaagtatct gtagcgctca
     3601 atgaagataa gagcagaaaa gaaatggtag aagaatatat tttgtatcag atcgaagcaa
     3661 gcggggaatc aggaatccca ttgagcgctt tgggcaatcg gatcagactg aaattcgcgg
     3721 attttaaagt gagagattat ggttattcac aatttaagca atacattcaa agctttccgg
     3781 aagtggaact tcaaggagaa ggagaaagga cgaaagcggt gtatgcgccg gtcttgtaac
     3841 atgacaggct ttcgtttata atagccgtgt aacaaaagag ttgataaaag ccgatatgcc
     3901 cctgcaatat gggcggcgta aagttgggag tggaaacaga tgataaaagt aaaagaatac
     3961 agaggtcata taagaaactg ggaagaactt tgcgaaagac tggatattcc tcttgatctg
     4021 acaagggaag agcgtgagga acagatcctt gtgaaggcat atgaaacatg gggaaatgag
     4081 atggcagatc acatgcacgg gatgtttgcc tttgccctgt gggatgaagc ggaagagaaa
     4141 ctgttttgct taagagatca gtttggaaca aaaccgtttt attattacga aacggcggac
     4201 ggaaaactgc tttacggaac aacgatccga aagattatgg aacagccggg atttgtaaaa
     4261 gaattaaacg aagagatgct tcagctttat ttaagcctta cttatgtggc gggcgaaaat
     4321 acatttttcc gcggtctgaa aaagctgctt ccgggccggt atcttgtatg gcagaacgga
     4381 aagcttacga tagagcgtta ttggatgcct cagtttcatc cggacaatag ccgtacactg
     4441 gagcagtggg cagatgagat ccatacgacg ataggcgaga tcatgccgga agtaaaaaca
     4501 aaagaagagt atgcggagtc atttttatca ggcggcgtcg attcgtctta cgttttggca
     4561 atgtcggatg tagagacgac agattcgtgc ggatatgacg aggaacgctt tgacgaatcg
     4621 ggtctggcga aacagacggc tgatattttg ggaagaaaaa atcttcgatg ccggatcact
     4681 ccggaggaat atttcgctgt tgtcccgtat gtgatgtaca atatggaaca gccgcttgga
     4741 gacgcgtcgg cgatcgcgtt tgccattgcg tgcagagaaa ccgcaaagca tacgaaaatc
     4801 tgttattcag gtgaaggcgc agatgaattt ttcggcggat ataatatgta tcgcaacgca
     4861 gaaagatacg gggagaattt aaagacattt tatgtcggaa atacaaatat catgaaagag
     4921 gatgagaaga agcggatttt gaaaaaatac gatccggacg tacttccgat cgaacttgcg
     4981 cgggggattt acgaggaaac ggaaggtctt gatcctctga ctaaaatgtc tgatgtagat
     5041 attcagatct ggcttgaagg cgatatttat ctcaatgtag ataagatgag tctggcagcg
     5101 ggacttgaga taagaatgcc gctgacggac agaagaattt ttgacattgc atcaagaatg
     5161 ccgtcggagt acaaggtaaa tgaagaacag aataaagtcg ctcttcgtac agcggctgca
     5221 aaagtgcttc cggaagaaat tgcatttcgc aagaaactcg gatttattgt gccgattcgt
     5281 atctggatgg cggatgatcg gtataatgcg gacgtgagaa gactttttgc gagcgatatc
     5341 gcggaaaaat tctttcatgt tgaagagata cgggctattt ttgacgagta tgtaggaggc
     5401 aattcggata actggagaaa agtgtggaca atctatactt tcctcgtatg gtatgaagag
     5461 tattttgtga aaagataatg gggagaaaga aaagagtttt attttaagta taaatatttg
     5521 cgcgcgataa tgatgcgtgt atgatgatgt gaatttaata aggggatgcg tatatatgac
     5581 gacaaagaaa agcgtcaaga aaatgtattg cagaatgttt caatcagtta tgaaagccgg
     5641 aatttccact atcccgtggc ggacgccgaa attgatgaag ggagcgggag cggtccgcca
     5701 ccttcctgcg gcgatcaaac ggagagggtt aaaaaaggcg cttatcgtta caggtcctac
     5761 tctgatcggg cgagggcttc ttgacggagt gcttgaagag atggaagaac agggactttt
     5821 gtatgtcatc tacaataagg tagaacaaaa tcctaccgat gaaaatgtgg aagatgcggt
     5881 gaaagtgttt ctggaaaatg ggtgtgactg tatgcttgcg ctgggcggag gagcgcctct
     5941 tgactgtgcg aaagcggttg gagcgcgtat cgcaagaccg aacagacaga taaggcagct
     6001 gcaggggttg tttcgggttg tcaagcagat tccgatgttg tttgcggttc ctacaacggc
     6061 aggatccggc tcggaaacga cgatatcggc agttatcacg gatacagcga cgcaccataa
     6121 ggcggcgatc aatgatctgc ggcttatgcc aaagtatgcg gtgctcgatc ccgaactgac
     6181 gctgggactg ccgccggacg ttaccgctat gacggggatg gatgctttgt gtcatgcggt
     6241 ggaagcgtat acaaatgata aatataacag tgatacagag cgggaattgt gcaggaaagc
     6301 tgtcagactg atctatgaaa atctgctgaa agtatatgaa aacggaagcg atatcgaggc
     6361 gagacagaag atgcagcagg cggcgtttta tgcaggacgg gcatttacaa gaggaggagt
     6421 cggatatgtt catgcgatcg gccatgcggt cggcggattg tatggaacgc ctcatggact
     6481 ggcaatggcg atttttcttc cttatgtgat gcgggcatat ggagacgcgg catgtaagaa
     6541 actggcggaa ttaagtgatg tgtgcagact tacgacaccg caggagtctg acagtgtgaa
     6601 ggcggatgcg tttatcggat ggatcgaaga actgaaagtg aagatgcaga ttccgcaata
     6661 tccggagatg atccgaaaaa cggatgtgaa gcagatcgca aaatgggcgg aaaaagaggc
     6721 aaatccgctt tatccgacgc cggaagtgtg gaacagacgg gagtttgaaa gatttctctt
     6781 aaacgtgtgt gtacaaggct caagcgagaa agaccggcag tgttgtcagg ataaataaga
     6841 gagaaaggat tctgaaaaga tgataatcga tacacatgct cattatgatg atgagcagtt
     6901 tgatgaggac cgggaggaaa tcctggggaa gatgcaggat gcgggaatcg gcatgataat
     6961 ggatgcaggt tctacaatcc tgtcatggga taagatcgta aaactgacag aagagtatcc
     7021 gtttgtgtac ggggcgatcg gcgttcatcc cgacgaagtc ggaaatcttg atgaaactca
     7081 gtttgcaagg atggaacgtc ttcttgataa agaaaagatc aaggcggtcg gagaaatcgg
     7141 tctggattat tattgggata aggaaaatca tgatcttcaa aaagaatggt ttatcagaca
     7201 gctcgatctt gcacgaaaaa aagagaaacc ggtcattatc cacagcaggg aggctgccgc
     7261 agatacgatg gagattatga aagaatatgc gtcgggactg cgcggcgtga tacactgtta
     7321 ttcatactcg gcggagatgg caaaagagta tgtgaaaatg gggtattata tcggaattgg
     7381 cggagtggta acttttaaga atgcaaagaa actaaaacag gtcgttcagg agattccttt
     7441 ggagtcgatc gtactggaaa cggactgtcc gtaccttgcg cctgttccat atagaggaaa
     7501 gcgaaacagt tcgctttacc ttccgtatgt ggcggaagag atcgcggcac taaaaggcgc
     7561 agcagcagaa gacgtcgtgt tacagacgga ggaaaacgca cggaagttat acggacttta
     7621 gagaaaaacg gattttagag aaaaagaaaa ggtgcggggg aaagccaaag aatgataagc
     7681 cgcacggaac ttctgaaaaa acagatttgg gaaggaaacg tatgaaagaa ccaacgcttg
     7741 gcaatccgca gaatacgatt gcggtgcttc aaaaatataa ttttattttt caaaagaaat
     7801 tcggacagaa ttttttgatc gatacacatg ttttagataa gatcatccgt gcggcggaga
     7861 tcgggaaaga cgatctcgta cttgagatcg gtccgggaat aggaacgatg acccagtatt
     7921 tgtcatgtgc ggcaggaaaa gtaatcgcag tagagatcga cagagctttg atcccgattt
     7981 tggaagatac gcttgacggc tatgacaatg tgcgtgtgat caacgaagac gtgctcaaag
     8041 ttgatatcag aaagttggta gaagaagaga atgagggcag accgatcaaa gttgttgcaa
     8101 atctgcctta ttatattact acgccgatca ttatggggtt gtttgaaaat catgttccga
     8161 taaaaagtat tacggtcatg gtacagaaag aagtggcgga tcggatgcag gtcgggccgg
     8221 gaacaaaaga ctacggcgca ttgtctcttg cggtgcagta ttatgccaaa ccgtatattg
     8281 tggcaaatgt tccgcccaac tgttttatgc ccagaccgaa agtagggtcg gcggtgatac
     8341 gtttagaaag gcacgaacat ccgcctgtcg aagtgcggga tgaaaagctg atgtttcggg
     8401 taatccgcgc atcttttaat cagcggagaa aaacattggc gaacggcttg aaaaactccc
     8461 ctgagattga tttttcaaaa gaggagattg aaggggcgat agaaaaactt ggaaaagggg
     8521 cgagcgtgag aggcgaagct ttgacacttg cggagtttgc acagcttgcg aattattttt
     8581 gtgatctgag agaaaaatga ttacgtgctg aataaaacag ttcagcttgc gccattcgga
     8641 aaaccgcact gcgtgcttat cgtggctgcc gccacagttt tcctcataac cgggcgctca
     8701 tctcatccag atgcccgctc ataaaaacat gcaagcatgt tttatacttc gcggacatct
     8761 ggatggctta aaaaaacagt tcagcttgcg ccattcggaa aaccgcactg cgtgcttatc
     8821 gtggctgccg ccacagtttt cctcataacc gggcgctcac ctcatccaga tgcccgctca
     8881 taaaaacatg caagcatgtt ttatgcttcg cggacatctg gatggctgaa ctgtctattc
     8941 ataaatattt aatagacagt ttattgtata atttctttat ttttagtata ataaacaaat
     9001 ataccaaaat aaaaaggagg aagttgtatg gcaaaaaata tagacagcat attttttgat
     9061 attactccga agattgaaga acttgctctt aagtgcgagc agaataatgc gatcgagaag
     9121 gaattataca cgaagtatga agtgaaaaga ggtctccgcg acctgaatgg aaagggcgtg
     9181 cttgccggac ttacgaacat ttccgatgtg tgcgcatcaa aaatcgtaga cggaaagtct
     9241 gtaccgtgtg aggggaatct gtattacaga ggttataata tcaaagatct tgtcagagga
     9301 ttcctcgagg cggatcaccc gggatttgag gagacggcgt acttgcttct gttcggcgaa
     9361 cttccgaata aagcacagtt ggaagagttt cagaatatga tcgcagagcg ccgtatgctt
     9421 cctccgaact tcgtgcgtga cgtcatcatg aaagcgccga gccgcgatat gatgaacagc
     9481 attacgagaa gtatcctgca gctttattct tatgacgaga aagcggatga tacgagtatt
     9541 ccgaacgttc tccgtcagtg tctgaacctc atcagtcagt ttccgatgct gatggtatac
     9601 ggatatcatg cgtataatta ccgcagagga gaggatctct atatctatgc gccgaaagcg
     9661 gaactgtcca cagcggaaaa tattttgatg atgctccgcg aggatcgtaa atatacaaaa
     9721 cttgaggcag agatcctgga tatggctctt gtgcttcaca tggatcacgg cggcggaaac
     9781 aactcgactt ttacgactca cgttgtcacg tcatcgggaa cggatacata ttctacgatc
     9841 gcggctgcga tggcatcttt gaaaggaccg aagcacggcg gcgcgaacat caaagtgact
     9901 cagatgttcg aggatatgaa gtctgttttg acagactggg aagacgaaga tcagatccgg
     9961 gcatatctgg aggcgcttct tgataaaaaa gcgttcgaca ggaaaggatt gatctacggt
    10021 atgggacatg ccgtttattc gatctccgat ccgcgagcgg atatattcaa agggtttgtg
    10081 aaaaagcttg cgaaggaaaa aggccatgag gcagaatacg gtctttatga aaaagtagag
    10141 caccttgcgc ctgagatcat cggagaaaag agaaagatgt ataagggcgt taatgcaaac
    10201 gtcgattttt acagcggact tgtctacagt atgctcgatc ttccgccggc attgtacaca
    10261 ccgatttttg cggcggcgcg tattgtagga tggagcgcgc acaggttgga agagcttaaa
    10321 aatgtggata agatcatacg tcctgcgtat aagccgctcg caccataccg cgattatata
    10381 aaattagatg acaggtaagg aagcatcagt gacatgagag acgagtttta tttcccatca
    10441 aaagacggta atacagagat tcatacgata gaatggaaac cggagggcga ggtaaaagcg
    10501 gtattgcaga tctgccacgg aatggtggag tatatcaagc gatatgatga atttgccgaa
    10561 tttttatgcg gcaagggttt ctatgtcgta gggaatgatc accttggaca cgggaagtcg
    10621 gtacaggcga aatcagaata tggttttttc agtgaaaaat acggaaatgc atgcgttata
    10681 ggcgatattc atacacttcg gcagaggacg atgaagaaat atccgaacgt tccgtatttt
    10741 atgctcggac acagtatggg gtcttcgctt ttgcgtcagt atattcagat gtacggcaag
    10801 ggattgagcg gggcgatcat tatgggcgcg gttgccgatc ataaaaaggc gacgcttgtg
    10861 ttcgggaagc gcttatgcag gctgatggcg gctgtgcgcg gctggcatta cagaagtcat
    10921 tttgtggatc atttagttgt gggaaatttt aatcgaaaat tcaaaccggc gaggacaaga
    10981 gccgactggg tcacaagtga cagagaacat ttggacgctt atgtatccga cccgctctgc
    11041 tcatttgttt tcacggtaaa tgcatattac agcatgtttt cgggaatgat cagcatgcag
    11101 agaaaagagg gcgtctatat gattccgaag aatctgccga ttttgtttgc ggcaggttcg
    11161 gaagatccgg tgggaaactt cggaaaaggc gttcgtaaaa tatatgaaaa atacagggcg
    11221 gcagggataa gagacgtatc tttgcagctc tataccggcg accgtcacga gattttaaac
    11281 gaatcagacc gggaacaggt atatgacgat ttatttgagt ggcttgcagg acatatggaa
    11341 aataaagtgt tataatagaa ttgttattgc cgcgaaagcg tcggtgaaat ttgattgaag
    11401 gagttatctg atatggaatt gacaacgatt cgggaattat ttaaagacag agaatcatat
    11461 cttggcaaaa aagtaacgat aggaggctgg gttcgtagtc tcagagattc aaagacattt
    11521 ggtttcattg tagtaaatga cggttcttat tttgagccgc ttcagattgt atatcatgat
    11581 gagatggaga actttgcgga agtttctaaa ctgaacgtcg gagctgcgat tattgtgaaa
    11641 ggaacgcttg tcgctactcc gcaggcaaaa cagccgtttg agattcaggc ggatgaagtg
    11701 cagatcgagg gagcgtcggc ttcggattat ccgctgcaga agaaacgtca tacgtttgag
    11761 tatttgagaa cgatttctca tctgcgtccg agaacgaata catttcaggc tgtattccgt
    11821 gtgcgttctc tggcagcgta tgcgattcat aagttctttc aggagagagg gtttgtgtat
    11881 gtacatacgc cgctgatcac aggaagcgac tgtgaaggcg cgggcgagat gtttcgtgtt
    11941 acgactcttg atatggatca gatcccgaag aatgaggatg gtacagtaga ttatacaaaa
    12001 gatttcttcg gaaaagagac gagccttacg gtaagcggtc agttgaacgg agagacatat
    12061 gcgcaggcgt ttagaagtat ttatacattc ggcccgacat tccgcgcgga gaattcaaac
    12121 acaacccgtc atgcggcaga gttttggatg atcgaaccgg agattgcgtt tgcagatctt
    12181 gaagacgaca tggatctggc agagtctatg ctgaaatatg tcatcaatta tgttcttgaa
    12241 aacgcgccgg aagagatgaa tttctttaat tctttcgttg ataaaggact tctggagaga
    12301 ctgaataatg ttgcgaattc tgatttcgcc cgtgttactt acacagaggc gatcgagata
    12361 ctggagaaga acaacgataa gtttgagtat aaggtttcct ggggatgtga tcttcagaca
    12421 gagcatgaac gttatttgac agagcaggtg tataagcgtc ctgtatttgt tacagattac
    12481 ccgaaagaaa tcaaagcgtt ttacatgaaa caaaatgatg acggcaagac ggttgcggca
    12541 gttgattgtc ttgttccggg tatcggggaa atcatcggag gaagtcagag agaagacgac
    12601 tatgagaaac tcctggcccg catgaacgaa cttggtcttg atgaaaaaga ttatggcttt
    12661 tatcttgatc ttagaaaata cggttctacg cgtcatgcag gtttcggatt aggatttgaa
    12721 agatgcgtga tgtatttgac gggaatgtcg aatatccgtg atgtgattcc gttcccgaga
    12781 acagtaaata actgtgaact gtaattgcgt tttgcggtgt aagacgtaaa aaaatacatc
    12841 gaaataaata tagaataaaa ttataaagta tatcatgagg gcgcgacagt tgtgtggcgt
    12901 ccttttattg caatagcggc gagcgaaagt ccgggcctgc ccgggttcgt ggcagccggc
    12961 agaaaacagc attgttgaaa ggggagcggg cgtgaaattt attcatatag cagacgtaca
    13021 tctcggagcg cagccggagg cggctgtata tagtcaaagc agaggaagag aattgtggga
    13081 gtcatttgag aaaatacttg gggtatgtga agatgagaga accgaccttc ttcttattgc
    13141 cggagatctt ttccacaggc agcctcttgt aagagagcta aaagaggtca attatctttt
    13201 ttcagaactg acagctacta aagttgtgct catagcgggc aatcatgatc atctgcagaa
    13261 ggattctaat tatcgttcat ttgagtggaa tgataatgta tatcctttgt tcgggaagaa
    13321 attggaatat gtcgattttc cggaattgga aacagcggtg tatggactca gttactatga
    13381 acgggaaatt tgtcagccgc tttatgacga tgtggctgca gcaggcattg aaaagaatga
    13441 aattctgttg gctcacggag gtgatgacag gcatattccg tttgataaaa agaagctgag
    13501 tcgttccgga ttttcatata tcgccctggg acatattcat aaaccgcagg ctttgcaaaa
    13561 agataagatg atttatgcgg gagcgcttga accgatcgac caaaacgacg tcgggcagca
    13621 tggatatgta aaaggggagc tcaaagacgg aaaagcggcg attcagtgga taccgtttgc
    13681 ggggagagag tatatccaca gttctgtcga agtagaaaga agcgatacgg aaggaagtat
    13741 aagaaaaagg gtaaagcaac ttataaatga gtatggaaat gaaaatattt acaaaattac
    13801 tcttgcggga aaaagagatc ccgatattgc ctttgaagtg aatcatttgg cggaggaagg
    13861 atgcgtgttg gagattttgg acgagacaat tccggcatat gattttgaaa agctttatgc
    13921 agagaacaag gagactcttc ttggcagata tatcgagaaa tttgcaggtt gtgaagaggg
    13981 aagtgttgag tattgtgcgc tttgtgaagg cgtggaagcg cttttgacgg gaaacagggg
    14041 atagagaaaa tggtaatcag agaactttat gttaaaaatt tcggaaaatt gtcggagaaa
    14101 catttttatt ttagggatgg agtacaggtc atcagcggag aaaatgagtt tggaaagacg
    14161 acgcttcatg cgtttgtgaa agcgatgctg tttggtttgg agcggggaag aggaagagcg
    14221 gcggcgaaag atgattttac aaagtatgag ccgcgcagcg gcggcaggta tgccggtgtc
    14281 atgcggtttg actgcggagg gagacatttt cgtcttgaac gtacattcgg aacaggggtt
    14341 aaaaattcta agtcggcggc tctgatctgt gaggatgacg gagaagaatt gtcggtggag
    14401 catggggatt tggaaatgct tctgggcgga ttgactgcgg agttgtttga cagtacggtt
    14461 tcggtcggac agttgaaaag cagaccgggg gaggctttgt ccgatgcgct ggaaaattat
    14521 gcggcgaatt attatgagac ggggggaaca gaacttgatc taagcggcgc agtgcagata
    14581 ttgagagaaa aaagaaaaaa gacagatcgg ttcatccggg aagaagcgga taaagaagaa
    14641 cggaaaatac agaaaatgct tcaggaatgc gagtatttgg agcaggatat ggatgagatc
    14701 caaagagagt atgaagagag aaaacaagaa tgggagctgc ttgagaaaac gataaaaaat
    14761 caggaaaaag aaaaagagtc tgattttaat aagagatctg atgaagggac gaaggagagc
    14821 agaagtaaat attttattgc ggcagggctt ggaggtcttt ttaccggcgc ggcaggactt
    14881 ttatggagca gatttatggc ggaacagtcg gcagtgcctt ccgcaccgtt tgcatggatc
    14941 ggcgtttttg cttttgtgat cggcgtgtgc gctcttgcgg caggaggtta tgaagcggga
    15001 aaaaaatcag gaaagcgaaa aaaagaggac gcccgtgatg tgccttataa taattccgat
    15061 aaagacgacg agagaaagaa gaaacaaaaa cagcagctgg agtggcagat ggagcatctg
    15121 cgtttggaat ggaaggaaaa agagctgcgt tgtcaaaatg tgcgggagca atgtggagat
    15181 gtgaaagaaa gcgacgccgg aaaaaggctg agagagcaaa gagaggcgct tgtaatggca
    15241 gaagagattc ttgtgcggac tgcaaaggaa acgtctgatg tgatttcgca taagataaac
    15301 gcccgggctt cagaaatttt ttcggaaatc acagacggaa aatatcgaag tgtaaatata
    15361 caaaaaggcg cgggaatttc ggcatggaac ggtatggatc gcatatctgt ggaccggttg
    15421 agcgaaggaa cgttggaaca gatttatttt tcaataagaa tggcagcgtc ggaaatgctt
    15481 ttggaagaac cgatgccggt tattttagat gatgcgtttg cgttttatga cgataaaagg
    15541 cttgaatctg tcataaaatg gttgagccgg cagaaaaagc aggttataat attaagttgt
    15601 cacagcaggg aagccaaatt gctggaacat ttagtctaaa tgataactag atcagatgat
    15661 cataaatgag tgtaaagaga taaaaaggtt atttcagagg tggaattttc atgaagatag
    15721 cattaccgcg aaaagtgatg atgattataa aaaatttgca gcttcacggc tatgaagcgt
    15781 atgctgtggg aggctgcgtg cgcgattcga tccttgcgcg cagaccgaag gactgggata
    15841 ttacaacttc cgcgcgcccg gaggaagtga agaagctttt caggcggaca gtagatacgg
    15901 ggattgaaca tggaacggtt accgtgctgc ttggaagaga cggttatgaa gttacgacat
    15961 acagaataga cggggcgtat gaagacagcc gccatccgaa agaggttttg tttacaaacc
    16021 gattagagga agatctgcgc cgcagagatt ttacgatcaa tgcaatggca tataatgatg
    16081 aagtgcgcct tgtagatgtg ttcggaggaa tgcaggattt aaaccatcat ttgatccggt
    16141 gtgtgggaga tccgagagaa cgtttttcgg aagatgctct tcgcattttg cgggcggtgc
    16201 gtttttccgc tcagcttaat ttcccgatag aagaggaaac ggctgaggcg gtcagggaac
    16261 ttgcccctac actggaaaat atcagtgcag agcgcattca ggcagagctt gtaaagcttc
    16321 ttgtgtcggc tcatccggaa cggatacaag atgcgtatga acttgggatc acgaggatca
    16381 ttctgccgga gtgggatgct atggcgggag tggagcagaa tactccccat cataaatatg
    16441 atgtggcaca gcacacgctc catgcattaa agtatgtaaa gcgtgataag atactgcgtc
    16501 ttacgatgct gtttcatgat atgggaaaac cggcgagaaa aacaacggat gaaaacggaa
    16561 gagatcattt taaaggacat gcgctggtga gcgaagcgat cgccagggac gtgatgcagc
    16621 gtttaaaatt tgataatgac acgattaaaa aagtgacgcg tctcgtatgt tttcatgatt
    16681 accgcacaga ggcgacgccg gcaaacgtgc gccgagcaat gaatcggatc ggcgttgatc
    16741 tgtttccgta ttatctggca gtgcgcatgg cagatgcgaa agcgcaaagt tcttacagaa
    16801 ggcgggaaaa gattgagaat atagtggcga tgagaaagtt ctatcaggaa gcgatcataa
    16861 aggatgaatg cgttactctc aggcagctcg ctgtaagcgg acgggatctg atgaatcttg
    16921 gcatgagacc cggcgtagag ataggaagga tgttaagcga gcttttggag tatgtgatag
    16981 ataatccgga aaaaaatgat aagaaaatat tatgcaacta tgtaaagaaa aaaatatctc
    17041 aaaggaataa aactgtctaa tcggaaaaag aaaaggaagg ttataattgc cctctgccgt
    17101 tcatacatta gagagagcta taatgtagaa aacagggggc agttatgaga gaagaatatg
    17161 aactggaagt tttggaaaag tatgatatag aagtgaaaag cacccggcgg ataaggggtg
    17221 cgtttttttg tataggcagc gagctaaagt ccgggcgtgc cggagacttt ggcgactgct
    17281 tgcaatcccg tagtgttcct tttggcgcgg cgggtgatta cgatacgaaa gaaaggccga
    17341 tgctgctcaa agagacaaaa ctgtcagagc ggcgcgccct ttttttatat gacgctctaa
    17401 gtgaaataga aagatgtgga aatgtaaaag ttgacacacc tgtctttacg aaagaaggcg
    17461 aacttcgagc cgcttcccgg gaaggagctt tttatatgct gaaaaaatgg tatcagggaa
    17521 gagagtgcga cgttcgtcag gaacaggacg ttgtacgggc ggcggcaaaa cttgccgttt
    17581 tgcataaaga tatggaaaaa ctggctgtaa aaatccggcc cggagcggat gtacagggaa
    17641 aaaatccggt ggaagaggtg cgaagatata acagggaatt aaaaaagatc cgttcattta
    17701 ttcgttccag acctgttaaa aatgattttg aaattttgtt tttggaaaat tttgaaaaaa
    17761 tgtaccgtac ggcggacgat attctcgcca ggatggaaac ttccggctgc agaaagcttt
    17821 ttgaagagag cgtatcaaaa gggagcgtcg tacacggaga ttataattat cataatctga
    17881 tcatgctcag agacgatatt gccgtaactg attttgaaca tatgcatacg gatatacaga
    17941 taaaagattt ctgttatttt ttaagaaaag caatggaaaa aaatcaatgg aaacaaaaaa
    18001 caggacaaaa aatattggaa gcatatgaag aagtacgtcc tttgtcggaa agggagaaag
    18061 agtttgcggc gctgagtctt gcatatcccg gaaagtttaa aaagatcgcg ggcagctatt
    18121 accggtcgaa taaggcgcac ctctcggaaa agaatgtaga aaagctacag atatgcatcc
    18181 gtcagacaga agagaaatat gagttcctta gtcggatatt tcctttgaat ttataaataa
    18241 ttgttgtata ataaaacata tcgggcatat aggggcggat ataaaattta tattagttta
    18301 ggaggtagaa atatggacta caaggcgagg tatgaagaat ggatttctaa ttcatacttt
    18361 gatgaagata cgagagcgga gcttgaaagt atcaaagagg atgaaaatga aataaaggaa
    18421 cgattttaca tggatctgga gttcggtact gcaggactga gaggcatcat cggcgcggga
    18481 acaaaccgta tgaatattta tacagtacgc aaagctacac agggactggc aaactatatc
    18541 atgaaaaacg acggtcaggg aaaaggcgtt gcgattgcgt atgattcccg tcatatgtcc
    18601 acggaatttg ccaaagaggc tgcatgttgt ctggctgcaa acgggatcaa agcatatata
    18661 tttgaatcgc tgcgcccgac gccggagtta tcttatgcgg tgagaaaact cggatgtatc
    18721 gcaggcatca atattacggc aagccataat ccgccggagt ataacggcta taaagtatac
    18781 tgggaagacg gcgcgcagat cacgcctccg cacgataaag gaattatgga cgaagtgaag
    18841 gctgtggaag attacacaac aatgaagacg atgacggaag aggatgcgaa agcggccgga
    18901 ctttacgaag tgatcggcgc ggaagttgac gatgcttata ttgcggaact gaaaaagcag
    18961 gtgcttcata aagatgcgat cgaagcagcg ggcaaagatc ttaagatcgt gtatacgccg
    19021 cttcacggaa cgggaaacat tccggctcgc cgtattttga aagagcttgg ttttgagaac
    19081 gtatatgtcg taaaagaaca ggaactgccg gacggagact tcccgacagt ttcctatccg
    19141 aacccggaag ccgaagaggc gtttgaactc ggattaaaac tggcgaaaga gatcgatgcg
    19201 gatatcgttc ttgcgacaga tccggatgcg gacaggctcg gcgtgcgtgt gaaagataaa
    19261 gacggagtat atcatgatct gaccggaaat atgtcgggat gccttcttgc ggattacgag
    19321 atcggacaga gaaaagaatt aaaaggtctg cctgaagacg gatatcttat caagacgatc
    19381 gtgacttcca atatggcgga tgcgatcgca gagcattata atgtcggatt gatcgagtgt
    19441 ctgacaggat ttaagtatat cggacagcag attctcggat tcgagacaaa aggaaaagga
    19501 gagtatctgt ttggatttga ggaaagttac ggatgtctga tcggaacaca tgcaagagat
    19561 aaagatgcga tcgtcgctac gatggctctg tgtgaagctg ccgcatatta ccgcacaaaa
    19621 gggatgacgt tgtgggatgc gatgatcgag atgtatgagc gttacggata ttacaaagac
    19681 gacatccagt ctatcacaat gaaaggaatc gaaggactcg agaagattcg tcagatcctt
    19741 gccacattaa gaagcgagcc tccgaaagaa atcgcaggat ataaagtact taaagtgaga
    19801 gattatcagg cggatacgat tcgggatatg gagaccggag aagttacggc tacaggactt
    19861 ccgaattcaa atgtacttta ctatgagctg cctgacggag cgtgggtatg cgtaagacct
    19921 tcgggaacag agccaaaagt gaaattctac tacggcgtga aaggtacttc tctgacagat
    19981 gcggatgtaa aatccgacga aatgggaaaa gccataatgg aaatggtgaa aaatcttgac
    20041 aaagctgtgt aaacccgata taacaatatt taaaggtgga aatccgtgtg cgttcggaat
    20101 cttccccttt aaaatgaata acatgtattg tggtacaatt aagaggagga tttacctatg
    20161 aacaagacag aattgattac agcaatcgca gagagcgctg acatttcaaa aaaagattct
    20221 gaaaaagcgc tgaaagcttt tgttgatgtt gttaccgaag aacttaaaaa aggcgagaag
    20281 gttcagcttg taggctttgg tacatttgaa gtgagtgaaa gagctgcaag agagggaaga
    20341 aacccgcaga caggaaagac aatgaagatc gaggcttgca aagctccgaa atttaaagca
    20401 ggaaaagctc ttaaagacgc tatcaattaa tcaattcgtt tgaacgggca gataaaaagg
    20461 cacttacggg tatcatcgta agtgccttgt tgcttctgtt tttactgaaa acagaggcgg
    20521 atccggacat tattgaagga gagaaaaatg cggcttgata aatttttaaa agtatcccgc
    20581 cttattaaga ggcggactgt agcaaatgaa gcatgtgatg cagggcgcgt ttccgtcaac
    20641 ggaaaaccgg caaaagcatc tgtgcaggta aaaccgggcg atgtgatcga gatctatttc
    20701 ggaacaagag aagtgaaagc ggaagtgctg aagctggaag atacgacgaa gaaggaaaaa
    20761 gcagaggaat tgttccggta tttgtagaga aagagaaaag aaaagcataa atgaaaacaa
    20821 cctctcatat atataaccat gctgtaaaaa gttcggaggc gggagggagt tttcatggaa
    20881 gaacagcgta ttgcgaaggc gcataaatta gttgtaaata acagaaagac gagtacggtg
    20941 acaggggtgc ttgacgtact gtcttttgat ctaaacgaga tacttcttga aacagagcag
    21001 gggatgctca tggtgaaagg cacggatctt catgtcaaca gactgaccgt ggaaaaaggc
    21061 gaggtagatc tgtcgggcaa tattgacagt attgcttatt caagcgtaac gccgcaggcg
    21121 aaacaggggg acagctttct gacgaagctg tttaagtagg atatgatacc cgggatcgga
    21181 gcagaagccg gaatctttct gtatgcggcg ctggcgggag tcgaagcggc ggcagcttat
    21241 catgtacttg tttgttttcg gaagcttgtt cctcacagcg gctgggcggt cagtctggag
    21301 gattttattt actggcttgc tgcgagcatc tatatttttc ggaaaatgta tgacacaaca
    21361 tacgggagca tccgctggtt ttttgtgttt gggatgctgt gcggagcggc ggcagggcat
    21421 gcggggatca gaaagattgt gaaactatgt gccgaaagat gcggaaaaaa ggaaaaaacg
    21481 gaaaaatcgt gcagaaaagc aaggaaagaa aatagaaaat aagtcttgaa atgagtcaga
    21541 aaagaagata caatattact taacactggg gagtgtaaag aaagatgaac agggtgagat
    21601 aaatgaacgg caagaagaaa agaacggcgg acagaaggaa tgccggacgc agaagatcgg
    21661 cacatctgca cagttataag aaaagcgttt tgatgatttg tacggttctt gtgtttttat
    21721 caggcgtact tgcggtgggc gcggtaaagc tttatgcaaa gaacaacgcg tataaggctc
    21781 aggaagagga actggcggcg cagatcaaag aggaagagaa gcgctcggaa gaagtgaaag
    21841 aatacgaaaa gtatgttcag acagacgagt atataaaaga gacggcagaa gataaattag
    21901 gacttgtgaa tccgaatgag attattttta aaccgtcgaa taaaaataaa taagacgaaa
    21961 aacaaacaga gcaaaaaagc cagacaggca tgtacgaaag ataactccgg ggaagatttc
    22021 ccggagtttt tttattgacg gcggcgagtt gaagtccggg cctgttcaag acattgacgg
    22081 ctgtctgtaa ttccggaata tgactcttgg cattttcggt gatgccgctt aagaaaatac
    22141 cgtgttacat taagacggga aagtcagatt gtaagaaagg ggatatataa tgaaagaagg
    22201 acaggctttg cagggaagtc cttatgtaga acagattgaa aaatatgcaa gttcgctcaa
    22261 acagctttcc catacatttt tagagttaga ggcacacaaa gaagagtttt cgggagccga
    22321 tattgacggg atgtttgacc gggaagaaaa atttcgtcct gaacctgaac agtatacaga
    22381 attac
//
LOCUS       DBMDXF010000005        21114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_787_length_21114_cov_2.154333, whole genome shotgun sequence.
ACCESSION   DBMDXF010000005 DBMDXF010000000
VERSION     DBMDXF010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_21114_cov_2.154333"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="ACZPD4_00465"
     CDS             <1..1608
                     /locus_tag="ACZPD4_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1377584.1"
                     /translation="VPSKSGDYSVSFNGKGGVLVYDDAWNEIKKYYPEEADGSRVVLS
                     LEQGKTYHFAVAALEKDMQWEIESAKTKNGFVYTNLADNTVEILKYTGTESNVTIPDK
                     IDNKVVKTLGAESFTENETVVGVTIPAQVTNLQYGAFASCTNLKKVTFAQGSQLKKIK
                     EETFEHCQKLEEIHIPDSVTQIEKGAFYYCRSLSKLTLGKKLEVIDNNAFEGCSSLKQ
                     IEIPDSVESIGEGAFTYCTSLEHVTIGNKLAYVAKSAFSWCNLTEITWGDGIAKIGDS
                     AFACNQKLTTVSIPDTVTELEYKAFAGCVELSDIEIPDSVEAIGGYAFEKWDIYNEGG
                     DTAWYDAQADGDVYAGKVYYKYKGTIAEGGTVNLKAGTKGIAGYAFLDQINLTGIEIP
                     DSVTNIGDYAFVGCEKLNKVTVPASVTKIGEKALGYLTSGKGGQVYKLEGFTIRGVAG
                     SAAEKYAKENEFNFEAYTPEYIRGDVDADRKVSIGDVRMTLRSICKKAELNGTQKLAA
                     DVEKDGTVDIKDLRKILRYVCGKIEYL"
     gene            complement(1907..2506)
                     /locus_tag="ACZPD4_00470"
     CDS             complement(1907..2506)
                     /locus_tag="ACZPD4_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1377585.1"
                     /translation="MSKVDDNKKQKESTLLETAFHLFTGKGFAKTTISDIVQQAGLAK
                     GTFYLYFKDKYDLRDKLIVYKANQLFDDAHRALEKANVSSFEDELLFTTDYIIERFQK
                     NHFFMEFIAKNLSWGIFKSVFTNGDPSFSSQFYDHYMTALKKYNVNCPAPELLLFTMI
                     ELIGSTSYNCIHNSQPVSMEEYLPYLHRSLHHILLAFTE"
     gene            2685..4772
                     /locus_tag="ACZPD4_00475"
     CDS             2685..4772
                     /locus_tag="ACZPD4_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1377586.1"
                     /translation="MVKAGKWIAKHRVLILIVSALLMIPSFFGIVNTRVNYDLLSYLP
                     EHLETVKGQDILVDEYGMGAFSMVVVEDMELKDVQKLEEKFSEVPHVKDVLWYDDAAD
                     ISLPVEMLPKDIREAFFKGEATMMLALFDNTTSSDEAMDAVTQMRQIADKQCFISGMT
                     GVVTDIKNLCLHELPIYVVIAVLCCLLILELTSTSFLVPILFLISIGAAIVYNLGSNI
                     FLGETSYITKALTAILQLGVTMDYSIFLLNSYEENKKRFPGEKERAMGHAIANTFKSV
                     VGSSVTTIAGFAALCAMTFTLGRDLGIVMAKGVVMGVLCCVTLLPSLVLVFDKWIEKT
                     THRPLFSNMNKPSEFITKHYAVWIVLFLVLLFPAIYGNNHTKIYYNIAQSLPGSLDSN
                     VANEKLEKDFEMGNMHMILMDKNMDGVQKQKMLDQVDEVDGVKWTISMNSLIGPSVPD
                     SMIPDDIKSMLKSDHYELAFICSEYASATDQVNTQIAQIDDIVKSYDDDAMVIGEAPL
                     MKDLQDVTDVDLTMVNVLSMAAIFLIIMIVFKSISLPVILVSVIEFAIAVNMAFPYYM
                     GTELPFVASIVIGTIQLGATVDYAILMTSRYQKERGRGRSKKEAIAIAHKTSMPSIIS
                     SGLSFFAATFGVACYSKVDMIGSICTLLARGAIISMIVVILVLPAMFMIFDKIICKTS
                     IGFLGEKAKKTAQ"
     gene            4829..7234
                     /locus_tag="ACZPD4_00480"
     CDS             4829..7234
                     /locus_tag="ACZPD4_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377587.1"
                     /translation="MNKNRRMKHVVAASLAFGAAALTGFGQIAPAAVYAAEQTETVKT
                     ESTKSGEQADEGLFKEETVYVNADANGAVKLITVSDWLKNAGTTESLKDVSELDGIKN
                     IKGEETFSESGDNLTWDTNGADIYYQGTTEKELPVSVKLTYYLDGKKISPSELKGKSG
                     HLKIKIDYTNNTKKSVNVSGKSEELSSPFVMMTGMILPNETFSNVAIDNGKVVSDGNR
                     NIVLGFAVPGMKESLGMNDFSQSSSQVSLPESLEISADVTDFTMSSTYTVALSDILED
                     LNVKEIVDYSSLQSALDELENAALELVSGTDTLSSGAAQLSSGAAELEDGIKKYTDGT
                     DTLANGVVSYVNGEQQLADGAKSLTELSAGLSQVQSAVSQLSAATDGSLTSVNEKDIK
                     AGAQKLADGTQQLVQVLGTEAVQAVMQQVTSMIEMGKALIEETKGLETSLQNGIVTPV
                     QDLAAQAQAISGELQKISDLQTNLTNACTAVNTAVNENNAVIDADNGQIAANNEKITS
                     AKTKAISSSQTIQQAVDSLTAKRNEIAQNDPQSAALADLDNAIQALNEAKNAAAEVTD
                     MPELSSIEGKLNPVQADLSGVDVQAVQNAVGKMKVDLFTLKLAAAGMNAKLPEVEEKL
                     DAIQKAGENLPLDQLGALTGKIDDLNTGMQGLNSGIGSLHTNLSSLDQSLAVSVPKAA
                     SGIAQLNGGFTQLGSFNSQLTAGAEQLKENSSVLKNGVSALNSGAKTLSEGAVTLADG
                     MKTFDAEGTSKLKTTVEGAVGELMNRLEALTSKECSYDTFSGKDSAMDGNVKFVIETE
                     AIE"
     gene            complement(7310..9172)
                     /gene="asnB"
                     /locus_tag="ACZPD4_00485"
     CDS             complement(7310..9172)
                     /gene="asnB"
                     /locus_tag="ACZPD4_00485"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1377588.1"
                     /translation="MCGIAGFFNPSADYTKEKNYRNHILEMMNQVQKHRGPDDEGTFL
                     SPQCGLAHVRLNIIDLVTGHQPMRRVRGDHSCVIAYNGEIYNAPELKAELISEGEQFL
                     TSGDTEVILAGFMRCGPSFIKKLNGIFAIALWDESCQTLHLFRDRLGVKPLFYTKKDE
                     TTIFSSEIKGLFAYPDIVPELDRNSLNEIFALGPAKTYGKGVFKNILEVLPGEHISIS
                     HTSFKRQFYWKLSSCLHADSPSDTIEKTRWLLTDSIKKQMLSDVPICTFLSGGIDSSL
                     VTAVCAGHLAKSGEKLNTFSFDFTENKTYFKSNTFQPSQDRPFAEQMAELYETNHHFL
                     ECSSQDQLDYLYKAVDARDLPCMADVESSMLYFCSLVKQFNSVVLTGECADEIFGGYP
                     WFHKKSSFETNMFPWSPSMQPRQALLDDDFIHELNMEEYALNAYEQTIRETPVLPDDS
                     PEEKRRREIAYLNIRWFMATLLDRMDRAGMYSGLEARVPFADYRIVEYIFNIPWDIKC
                     PDGIVKGLLRHAGEGILPKEILWRRKSPYPKTYDPHYEALLADQLKEVLASPNAPIRT
                     FLDKKKVLRFLDTPSDYGKPWYGQLMAGPQMLAYMLQVNYWMEKWKHPLPSGCS"
     gene            complement(9190..9783)
                     /locus_tag="ACZPD4_00490"
     CDS             complement(9190..9783)
                     /locus_tag="ACZPD4_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HIR1377589.1"
                     /translation="MSLKETQIGVAFTGSFCTYKKAFLELQKLSNCCGSVQTVFSTAA
                     ASTDSRFGSAESFYRRAEEITGNKPMMTISEAEPIGPKNLFDALVILPCTGNTLAKLA
                     NGITDTPALMAAKAHLRNNKPLLLSLSTNDALGMNMKNIGLLLNTKNIYFVPFGQDDP
                     EKKPNSMTAHTDLLIPALEAALENRQLQPVIRDIVKK"
     gene            complement(9815..10711)
                     /locus_tag="ACZPD4_00495"
     CDS             complement(9815..10711)
                     /locus_tag="ACZPD4_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HIR1377590.1"
                     /translation="MSSFSYDFSVLGGDARQVFLAEMLAESGRSLCHYALASQPNNCS
                     SVSEHVSIVREAPSLEEACIHSGSVICPFPFTKNGADVSVENSIYRFSIQTLLHLLRP
                     GQSLFAGGIPPSFKKEAAENNVAVFDYADDISLPVYNSISTAEGAICEAILHSPFNLH
                     KSRCAVLGFGKCGHSIVYRLKGLSANVSVFTDNAEESARASVIADKSFPLKSLSDHAG
                     DFRFIFNTVPAQFINEETLKNVDLSALIIDIASSPGGVDYDAAQRLGISALHCLGLPG
                     KYAPASSARHLKQFIESKLYLS"
     gene            11112..11810
                     /locus_tag="ACZPD4_00500"
     CDS             11112..11810
                     /locus_tag="ACZPD4_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1377591.1"
                     /translation="MYNILVCDDDKEIVEAIEIYLTQEGYRILKAYDGTEALEMLERE
                     EVHLLLLDVMMPKLDGIRATLKIREKNSIPIIILSAKSEDADKILGLNVGADDYVTKP
                     FSPLELVARVKSQLRRYTQLGGTAQNENDKIYSVGGLRINDDLKEVTVDGELVRLTPI
                     EYNILLLLMKNQGRVFSINQIYEMIWNEEAIGADNTVAVHIRHIREKIEINPKEPRYL
                     KVVWGVGYKIEKVQ"
     gene            11842..14481
                     /locus_tag="ACZPD4_00505"
     CDS             11842..14481
                     /locus_tag="ACZPD4_00505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1377592.1"
                     /translation="MKGWYRRTAMKAVVLIVGVLTGAMFFTSLIVTTRLTGTLNPAET
                     VKLIGEPYEESSDFSNTVESYMLQVLEQFQLKTLFESDGAYNPDKVIDVMSYFGASGA
                     DAENHSGLVYRLSDLIEWSKAYSVESGGVYDKNAVIVCKQPSGKYYYYYTEEFLNQLE
                     TGELQLVLEDDRMDVESFLNDLSNGTLTSSGVSDKISIYDAEGSKLYTDCWNYGDSVR
                     ENYAPLGAANLLQVVNERPELNGKLMVIYDHLASVLTSINDKYSRYKSGWEHLEEGNT
                     NFTYLYIDQDTKKVTTNKSEYANLMAKGESGIKTNIEECIADMKSGKNVKYIFVYPKL
                     KDFETNMDILPTNEWDMVRSYDTDKTYDSIFAVAVDTSYPVKDMFYERDRIYEKNIPL
                     LRGGFVILCLSGILFLISFVMSGLMAGRKDGDNEVHLTGFDRWKTEIAAVAVIGVWAV
                     GTAVIVGMGMIDSDNVYNMVETLDYYGARYMSDMPEVYSMLFVQKVSFLEIAGAFLYG
                     AFTFICFFAGYMSLIRRMKAKSLWNGSLLCMILSFCKRIVDSSNDVWRTVLWISAFLV
                     IHWITFFVRNVPMALAALAADGVTFWIVVGSMLAKVRIKKGIEQIASGNLEYRIELKG
                     LRGTERNIAENVNDIGSGLNRVIDEAMRNERLKTDLITNVSHDIKTPLTSIINYVDIL
                     KRSDIADEKILGYLDILEVKAQRLKTLTEDVVEASKVSSGNITLEYMDIDFREMVQQT
                     EGEMAEKFAARNLSVVLNLPEEAAVIHVDGRRMWRVLENIFGNAAKYAMPGTRVYADL
                     SVSESEVKFSLKNVSEQQLNISADELTERFIRGDISRSTEGSGLGLSIAKSLTEMQGG
                     VFELYLDGDLFKVDIRFPRIKPM"
     gene            complement(14534..15901)
                     /locus_tag="ACZPD4_00510"
     CDS             complement(14534..15901)
                     /locus_tag="ACZPD4_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1377593.1"
                     /translation="MTASKSKKNKYEIDMCNGTVLDKLISFSIPLMLSSILQLLFNAV
                     DIVVVGRFSGSRALAAVGSTTALINVFTNLFIGVSLGANVLAARFYATKQEKQMSETV
                     HTAVTFALISGIVMVFVGLFFSKAALLLMDTPSDVIGQSALYMRIYFAGMPFFMLYNY
                     GAAILRAVGDTKRPLFFLIIAGIVNAALNLFLVIVLHLGVAGVAIATVIAQMISCLLV
                     LRCLYRSDGSYQLRFSAMTIKWIYLKQIFQVGIPAGIQSTVINFSNVLLQSSVNSFGS
                     IAMAGYTAANNIFGFLYAAANSITQACMSFTSQNYGAGKWKRMDKVLRNCILLSVVVS
                     LILGGGSYLFGPELLKIYTSDPKVIQCGMEILLYTTVTYFMCGLMDLFPGALRGMGYS
                     AIPMLLSIIGTVGTRIVWIFMIFPRHRSLDVLFISYPASWLITIILQVICYFFVRKRI
                     HNPLP"
     gene            16249..18150
                     /gene="metG"
                     /locus_tag="ACZPD4_00515"
     CDS             16249..18150
                     /gene="metG"
                     /locus_tag="ACZPD4_00515"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1377594.1"
                     /translation="MSEKQKYYITTAIAYTSGKPHIGNTYEIVLADAIARYKRSQGYD
                     VFFQTGTDEHGQKIELKAEEAGVTPKEFVDGVSAEIKKIWDLMDTSYDKFIRTTDEDH
                     EKQVQKIFKKLYDQGDIYKGHYEGMYCTPCESFFTESQLVDGKCPDCGREVQPAKEEA
                     YFFKMSKYADRLIEHINTHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVD
                     FDDKHVVYVWLDALTNYITGIGYDADGDSTEQYAKLWPADLHLIGKDIIRFHTIYWPI
                     FLMALGLPLPKQVFGHPWLLQGDGKMSKSKGNVLYADELVDFFGVDAVRYFVLHEMPF
                     ENDGVITWELMVERLNSDLANTLGNLVNRTVSMTNKYFGGVVSDKGAAEDVDADLKAV
                     IDNTPKAVDAKMDGLRVADAITEIFNLFKRCNKYIDETMPWALAKDEEKKDRLETVLW
                     NLIQGISAGARLLESFMPSTSKKILDQLGDGHVTEKPEILFQRLDLEEVMKKVEELHP
                     HIEEAEEEEDVIDIEAKPEITFEQFGAMQFQVGEIIACEAVKKSKKLLCSQVKVGSQV
                     KQIVSGIKAHYTPEEMVGKKVMVLVNLKPAKLAGVLSEGMLLCAEDAEGNLALMTPEK
                     AMPAGAEIC"
     gene            18458..18985
                     /locus_tag="ACZPD4_00520"
     CDS             18458..18985
                     /locus_tag="ACZPD4_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1377595.1"
                     /translation="MSAGIVDIQRQLIKKAKLGDTKAFSQLYAEIYKDLYRFALYMTK
                     HTQDAEDAVSEAVISAYENISGLKKENSFKSWMFTILNNECKKILRKNGNTSNQVSEE
                     DAKNLGAEEIDFAQRHDVKKAFESLEEEEQTIVAFSVFGGYRSEEIAEMLGKNPGTVR
                     SRKSRAFGKMREMLD"
     gene            19074..21086
                     /locus_tag="ACZPD4_00525"
     CDS             19074..21086
                     /locus_tag="ACZPD4_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR1377596.1"
                     /translation="MSENKKETKNQDMIEKEMKEKMKQTVEDEKIPGSLEPEAVEKML
                     ETRKKKKSNNRRWKYASVAAAACVCLVVGAVGYTNMNAGKNERPGTVTEGRSEEDQGK
                     IALAKNYDEIKKYLKENEKRAKGQNGFGRADAAYMTDGAMAESSVAESTKDMAGADYS
                     DTNIRQEGVGEADTVKTDGKNLYILNDQTVEIVDVSQAEMKDLASIEIDENCFIREVF
                     VSDGRLVILYTESKCEEGKKAEDGIYKDYTCAAVYDVTDPANPREAGVISQSGQYHTM
                     RVKDGYVYLVSDFYTYYDSSVSNESDYIPQIQGSLLRAEDIYMPQGTTGSQYTVISAF
                     ALSDPTEKLQTKAIFGNAGMCYVSENNIYITEEYYGKSETENIQTSIRKIAYDKGTLD
                     AVGQTKIDGVLNDSFSIDEYNGYLRIAATVIPSDYNNRIMPVPYVEEGGSDVIVEDEV
                     AVDNASIETNALYVLDENLEMTGSIQNLAKEETIHSARFMGDIGYFVTFKQIDPLFSV
                     DLSDPSNPKIIGELKIPGFSDYLHPYGDGKLLGIGLSVDEEGMTTEGVKLSMFDISDP
                     ANVKEMSTYVLENVLSTDAGYNYKAVFADAEKNLFGFMAYGDSIEYKMFTYDEAEGFK
                     EVFSKDIVNYGNVRGLYIGETFYLVAGNTVESFTLNGFEKIDDLVL"
BASE COUNT         6611 a         3611 c         5350 g         5542 t
ORIGIN      
        1 gtaccaagta aatccggaga ttattctgtt tcttttaacg gaaaaggcgg agttttagta
       61 tatgatgacg catggaatga gataaaaaaa tattatcctg aagaagcaga cggaagccgt
      121 gttgttcttt cgctggaaca gggaaaaacg tatcactttg ctgttgcagc attagagaaa
      181 gatatgcaat gggaaatcga atcagctaaa acaaaaaacg gattcgtata cacaaattta
      241 gctgataata cagtagagat tttgaaatat acaggaacgg aaagtaatgt aacaataccg
      301 gataagattg acaataaagt cgtaaaaact cttggagctg aatcatttac agagaatgag
      361 acggtagtag gagttacgat tccggcacaa gttacgaatc tgcagtacgg agcgtttgca
      421 tcatgtacga atttgaagaa agtaactttc gcacaaggca gtcaactgaa aaaaatcaaa
      481 gaagaaacat ttgaacactg tcaaaagtta gaagaaatac atattcccga cagtgttaca
      541 caaatagaga agggagcgtt ttattattgt cggtctttga gcaagttaac gctagggaag
      601 aagcttgagg taattgataa caacgccttt gaaggctgca gcagtctgaa acagattgaa
      661 attcctgatt ctgtggaaag tatcggagag ggagcattta catattgcac ttctcttgaa
      721 catgtgacga ttggaaataa actggcatat gtagcgaaaa gtgcattttc atggtgtaac
      781 ctgacggaaa ttacatgggg agacggaatt gcaaaaatcg gagacagtgc gtttgcttgt
      841 aaccaaaaac ttacgacggt ttcgattcct gatacagtaa cggaacttga atacaaagcg
      901 tttgccggat gtgtagagtt aagtgatatt gaaattcctg attcggtaga agcaatcgga
      961 ggctatgcat ttgagaagtg ggatatatat aatgaaggtg gggacactgc atggtatgac
     1021 gcacaggctg acggtgatgt gtatgccgga aaagtttact acaaatataa aggaacaatc
     1081 gcagaaggcg gtactgtcaa tctgaaagcc ggtacgaaag gaatcgcagg atatgcgttc
     1141 cttgatcaga taaatctgac aggaattgaa attcctgata gtgtaacgaa tatcggcgat
     1201 tatgcatttg tcggatgtga aaaattaaac aaagtgactg tccctgcttc tgtaacaaaa
     1261 atcggagaga aagctcttgg atatttgacg tcgggaaagg gaggacaggt ttataagtta
     1321 gaaggcttta cgatccgggg agtagccggt tcagctgcgg agaagtatgc gaaagaaaac
     1381 gaatttaatt ttgaggcgta tactcctgag tatattagag gagatgtgga tgccgacaga
     1441 aaagttagta tcggagacgt ccgtatgaca ttgcggagca tctgtaagaa ggcggaattg
     1501 aacggaacac agaagcttgc ggcagatgtg gaaaaagacg gaacagtaga tataaaagac
     1561 ttgcggaaga ttttaagata tgtctgcggt aaaattgaat atttgtaaag catattatta
     1621 agtgtattgt taaacgcatt ttgtgagtga atctcaaaag tgtaagaatg ggaagaaccc
     1681 tgctttgccg aaaggcggag cggggttctt cttatactgc gatcatccgt gaatgtgagc
     1741 gctgacgata tctccgcgat aactattctg tggatgccag tgatatctgc ggcgataact
     1801 attctgtgga tgccagtgat atctgcggtg ataactattc tgtgactgtc ggcgatatat
     1861 gttgtggtaa ttattctgta aatgtctgtg atatatgttg cggtaattat tctgtaaatg
     1921 caagtaatat gtgatgcagt gatctgtgca gatacggcag atattcttcc atactgacag
     1981 gctggctgtt atggatacag ttataacttg tagagccgat cagttctatc atcgtgaata
     2041 gaagcagttc cggagcaggg cagtttacat tgtatttttt caaagcggtc atgtagtgat
     2101 cgtagaattg gctggagaaa gaaggatctc cgtttgtaaa gacactttta aatatccccc
     2161 atgataagtt tttggcaata aactccatga agaagtggtt tttttgaaat cgttcgataa
     2221 tgtagtccgt agtaaagagc agttcatcct caaaactgct cacatttgct ttttcaagag
     2281 cgcggtgtgc atcgtcaaaa agttgatttg ccttatatac gatgagtttg tcacgcagat
     2341 cgtatttatc cttgaaatac aggtaaaaag ttccttttgc aaggccggcc tgttggacaa
     2401 tatcagaaat tgttgttttg gcaaatcctt ttcctgtgaa aagatgaaag gcagtttcga
     2461 gaagtgtgct ctctttctgc tttttattat catcaacttt gctcataata gatcccctct
     2521 tatttttgat gcttaatctt ttgttatttt tcctatatta tgaattgttt tattgagaaa
     2581 gtcaataaaa tgactaaaat tcatttttat cgctgaaaaa tattgactat tagtcatttt
     2641 tggaatatat tgtgtattat acaaaaaata aggagatgat ttcagtggtc aaagcaggca
     2701 agtggattgc aaagcatcgg gtgttgatct tgattgtcag cgcgctgctt atgattccgt
     2761 cttttttcgg aatcgtgaat acgagggtca actatgatct gctcagctat ctgcctgaac
     2821 atcttgaaac cgtgaagggg caggatattt tggtagatga atatggcatg ggagcgtttt
     2881 ccatggtcgt agtagaagat atggaactaa aggatgtaca gaagctggaa gaaaagttca
     2941 gcgaagtacc gcatgtaaaa gatgtgttat ggtacgatga tgccgcagat atcagtcttc
     3001 ccgtcgagat gctgccgaag gatataaggg aagccttttt caaaggagaa gcaacgatga
     3061 tgttggctct ttttgataat acgacatctt cggacgaggc aatggatgcg gtgacacaga
     3121 tgaggcagat tgcagataag caatgcttta taagcggaat gacaggtgtt gttacagata
     3181 ttaaaaatct ttgcctgcat gagctcccga tttatgtggt gatcgcagta ctctgctgtc
     3241 ttttgatatt ggaactgacg tccacgtcgt ttcttgtacc gatcttgttt ttgataagta
     3301 tcggagcggc gattgtatat aatctcggaa gcaatatctt tttgggagaa acgtcctata
     3361 ttacgaaagc gctgactgcg attttacagt taggcgtaac gatggattat tccattttcc
     3421 ttttgaatag ttatgaggaa aataagaaac ggtttccggg agaaaaagaa cgggcgatgg
     3481 ggcatgcgat cgcgaatacg tttaagtcgg ttgtcggaag ttcggtgaca acgatcgcag
     3541 gcttcgcggc gttgtgcgcc atgacgttta cacttggaag agatctcgga atcgtaatgg
     3601 caaaaggtgt tgttatggga gtgctctgtt gtgtgacgct gcttccttca cttgttcttg
     3661 tatttgacaa atggatagaa aaaacaacgc atagaccact gttttctaat atgaataaac
     3721 cgtcagagtt tattacgaaa cattatgctg tctggatcgt tttgttcctt gtccttttgt
     3781 tccctgcaat ttacggaaac aaccatacga agatctatta taatatcgct cagtctcttc
     3841 cgggatcgct tgacagtaat gtggcgaatg aaaaactgga aaaggatttt gaaatgggaa
     3901 acatgcatat gatcctcatg gataaaaata tggatggtgt gcagaaacag aaaatgctcg
     3961 atcaggttga tgaagttgac ggtgtaaaat ggacgatcag catgaactct ttgataggtc
     4021 cgtccgtgcc ggattcgatg atcccggacg atattaagag tatgttgaaa agcgatcact
     4081 atgagctggc atttatttgt tcggaatatg catctgcaac agatcaggta aatacacaga
     4141 tcgcacagat cgacgatatt gtgaaatcat atgacgatga cgctatggtg atcggggaag
     4201 cgcctttgat gaaagacctt caggatgtaa cggatgtaga ccttacaatg gtcaatgttc
     4261 tttcgatggc ggcgatcttt ctgatcatta tgatcgtatt taagtcaatt tcccttccgg
     4321 ttattcttgt atcggttatc gagtttgcaa tagcggtcaa catggcgttt ccatattata
     4381 tgggaacgga acttccgttt gtggcaagta ttgtgatcgg aacgattcag cttggagcga
     4441 ctgttgacta tgcgatcttg atgacgagcc gttatcagaa agaacgcgga cgcggaaggt
     4501 cgaaaaaaga agcgatcgcg atcgctcata aaacaagcat gccgtcaatt atcagcagcg
     4561 gtttaagttt ctttgcggcg acgttcggag tggcgtgcta ctctaaagta gacatgatcg
     4621 gttctatatg tacattgttg gcaaggggag cgatcatcag tatgatcgta gttatacttg
     4681 tacttccggc gatgtttatg atatttgata aaattatctg taagacatcg attggatttc
     4741 tcggagaaaa ggcgaagaaa acggcgcaat aaaaagtaat ggaaacaaaa agtaaaacta
     4801 tgaatgtcgg gaaaggatgt tggcagttat gaataaaaac agaagaatga aacatgtagt
     4861 agcggcttca ctggctttcg gagcggcggc gcttaccgga ttcggacaga tcgctccggc
     4921 agcagtatat gcggcagagc agacggaaac tgtgaaaaca gaaagtacaa aaagcggaga
     4981 acaggcagac gaaggcctgt ttaaagaaga aacggtttat gtcaatgcag atgcaaacgg
     5041 agcggtaaaa cttataacag tttcggattg gctgaaaaat gcgggaacga cagaaagttt
     5101 aaaagatgtc agcgaacttg acggtattaa aaatattaaa ggagaagaaa ccttttcgga
     5161 gtcgggcgat aatctgacgt gggatacaaa cggcgcagat atttattatc agggaacgac
     5221 tgaaaaagaa cttccggttt ccgtgaaact gacctattat ctggatggaa agaaaatcag
     5281 tccttcagag ctgaaaggaa aaagcggaca tctgaagatc aaaattgatt atacaaataa
     5341 cacaaagaaa tcggtgaatg taagcggaaa atccgaagaa ctaagcagtc cgtttgtaat
     5401 gatgacagga atgatacttc cgaatgagac gtttagcaat gtggcgatcg acaacggaaa
     5461 agtagtgtca gacgggaata gaaatatcgt acttggattt gctgttccgg gtatgaaaga
     5521 aagtctggga atgaatgatt tctcgcagtc gtcttcgcag gtatcgcttc ctgaaagtct
     5581 tgagataagc gcggacgtaa cggattttac gatgtcctct acttatacgg tcgcattgtc
     5641 cgatattttg gaagatttga atgtgaaaga aatcgttgat tacagttctt tgcaaagcgc
     5701 tcttgatgag ctggaaaatg cggcgttgga actggtaagc ggaacagata cgttgtcttc
     5761 cggggcggcg cagctttctt ccggcgcggc agaattagaa gacggcatca agaaatatac
     5821 agatggaacg gatacgttgg caaatggagt tgtatcttat gtaaatgggg aacaacagtt
     5881 agcagacggc gcaaaaagcc ttacggaatt gagcgcaggc ttatcccagg ttcagtcagc
     5941 agtgagtcag cttagcgcag ccacagacgg aagtttgaca agcgtgaatg agaaagatat
     6001 taaggcagga gcgcagaaat tggcagacgg aacgcagcag cttgtacagg ttctcggaac
     6061 agaggctgtt caggctgtga tgcagcaggt gacaagtatg atcgagatgg gaaaggcgct
     6121 cattgaggaa acgaaagggc tggaaacaag tctgcaaaac ggaatcgtaa ccccggttca
     6181 ggatcttgcg gcgcaggcac aggcaatttc aggagagctt cagaaaattt cagatttgca
     6241 gacgaatctt acaaatgcat gtactgccgt aaatactgcg gtaaacgaaa ataatgctgt
     6301 tattgatgcc gataatggac agattgcggc gaataacgaa aagattacgt cagctaagac
     6361 gaaagccatc tcaagttcac agacgattca acaggcggta gattccctga cggcgaagag
     6421 aaatgaaatc gcacaaaatg atcctcagtc agcggctctt gcagatcttg ataatgcgat
     6481 acaggcgctg aatgaagcga aaaacgccgc ggctgaagtg accgatatgc cggaactttc
     6541 ttctatagaa ggaaaactaa atccggtgca ggcggatctg tcaggcgtgg atgtgcaggc
     6601 tgttcaaaat gcagtaggaa aaatgaaagt agatctgttt acattaaaac ttgcggctgc
     6661 aggaatgaat gcaaaacttc ccgaagtaga agaaaaatta gacgcaatcc agaaagcggg
     6721 agaaaacctg cctttggatc aactgggagc gctgacaggt aagatcgacg atctaaatac
     6781 gggaatgcag gggcttaaca gtggaatcgg atctttgcat acgaatctat catctttaga
     6841 tcaatctctt gcagtatcag taccgaaagc ggcttccgga atcgcacagc taaacggagg
     6901 atttacacaa ttaggcagct ttaacagtca gcttacggca ggagcggagc agttaaaaga
     6961 aaacagcagc gtactgaaaa acggagtgtc ggctttgaac agcggagcga aaactttgtc
     7021 agaaggagct gtgacgctgg cggacggaat gaagacgttt gatgcagaag gaacgagtaa
     7081 actgaaaaca acggttgaag gtgcggtcgg agaattgatg aaccggttag aagcgctgac
     7141 atctaaagag tgcagctatg atacgttcag cggaaaagac agcgcgatgg acggtaatgt
     7201 aaaatttgta atcgaaacag aagcgattga ataaaagcag aaaaaatgcc tcccctgtct
     7261 gagccgcagg agaggcattt tttataagga caattaaata aacttctgtt caggagcatc
     7321 cacttgggag tggatgcttc catttttcca tccaataatt gacctgcagc atataagcaa
     7381 gcatctgagg tcctgccata agctgcccat accatggttt tccgtagtcg gaaggcgtat
     7441 caaggaaacg caatactttc tttttatcaa gaaatgttcg tataggagca ttgggggaag
     7501 cgaggacttc ttttagttga tctgcgagca atgcttcata atgaggatca taagtttttg
     7561 gataaggact tttgcgtctc cacaagatct cttttggcaa gatgccctct ccggcatggc
     7621 ggagcagacc ttttacaatt ccgtccggac atttgatgtc ccacgggata ttgaaaatgt
     7681 attctacgat ccgataatcg gcaaacggta cgcgggcttc aagtccgctg tacataccgg
     7741 cgcggtccat ccgatcaaga agagttgcca taaaccagcg gatattcagg taggcgatct
     7801 cacgccgacg tttttcttcg ggagaatcat caggtagaac cggcgtttca cgaatcgtct
     7861 gttcataggc gttaagggca tattcttcca tgttcagttc gtgaataaaa tcgtcgtcta
     7921 acaatgcttg ccgcggctgc atagaaggcg accatggaaa catattcgtt tcaaatgagc
     7981 tttttttatg aaaccaaggg tagcctccga aaatttcatc agcacattcg cctgtaagga
     8041 caacgctgtt gaactgtttg acaagtgaac agaaatacaa catagaggac tcgacgtcgg
     8101 ccatgcaggg gagatcccgc gcgtctacgg ctttgtacag ataatccagt tgatcctgac
     8161 tgctgcattc gaggaagtga tggtttgttt catataattc tgccatctgt tcggcaaacg
     8221 gacgatcctg acttggctgg aatgtattgg atttaaaata cgttttgttt tccgtgaaat
     8281 caaaagaaaa agtatttagt ttttcccctg attttgccag atgtccggcg cagacggcag
     8341 tgacgaggct gctgtctatt ccgccggaaa gaaacgtgca gatgggaacg tctgaaagca
     8401 tctgcttttt gatggaatct gtgagaagcc atctcgtttt ttcgattgta tcagaaggcg
     8461 aatcagcgtg gaggcagctg ctaagtttcc agtaaaactg tcttttgaaa gaagtgtgac
     8521 taatggaaat gtgttcgccg gggagcactt caagaatatt tttaaataca ccttttccgt
     8581 atgtttttgc aggacctaag gcgaatattt cattgaggct gttccggtca agttcgggaa
     8641 cgatatcggg ataggcaaat aatcccttga tttcggaaga aaaaatagta gtctcgtctt
     8701 tttttgtata aaagagaggt ttgacaccga gcctgtcgcg gaacagatga agcgtttgac
     8761 aagattcatc ccacagcgcg attgcaaaga ttccgtttaa ctttttaata aaagatggtc
     8821 cgcagcgcat aaatccggca aggattactt ctgtatcgcc ggacgtcaga aactgttcac
     8881 cttcagaaat caattctgct ttcagttccg gggcattata tatttcaccg ttatatgcaa
     8941 tgacacaact gtgatcgcct ctgacgcgtc tcatcggctg atgtccggtg actaagtcga
     9001 tgatgttcag acgaacatgg gcaagcccgc attgagggga gagaaacgta ccttcatcat
     9061 ccggcccacg gtgcttttga acttgattca tcatttctaa aatgtggttt ctgtagttct
     9121 tttcttttgt ataatccgca gatgggttaa aaaatccggc gattccgcac atagaaaatt
     9181 ctccttcttc tattttttga caatatcgcg tatgacaggc tgaagctgcc ggttctcaag
     9241 agcggcttcc aaagccggga taagcagatc ggtatgggca gtcattgaat tcggcttttt
     9301 ttcgggatca tcctgcccga acggaacaaa gtagatattt ttcgtattta aaagaagtcc
     9361 gatatttttc atattcatac cgagggcgtc gtttgtcgat aaagataaaa gaagcggttt
     9421 gttgttgcgt aaatgggctt ttgccgccat taaagcaggt gtgtcggtga ttccgtttgc
     9481 cagtttggca agcgtatttc cggtgcacgg aaggatcaca agtgcgtcga aaaggttttt
     9541 cggaccgatc ggctctgctt cggaaatagt catcatcggt ttatttcccg taatttcttc
     9601 tgcacgccta taaaatgatt cggcagaacc gaaacgggag tctgtggatg cggctgcggt
     9661 tgaaaagact gtctggacag agccacagca gtttgaaagt ttttgcagtt ccagaaatgc
     9721 ttttttgtaa gtgcagaacg agccggtaaa agcgactccg atctgcgttt cttttaagga
     9781 caaaggggat tcctcctttc attttgcgca tgaattatga gagatacagt tttgattcga
     9841 taaattgttt taagtgacgg gcagaagatg cgggagcata tttgccggga agtcctaagc
     9901 agtgcagagc agatatgccg agacgctgag ccgcgtcata atcgacccct ccgggggaag
     9961 aggcaatatc aataataaga gcagaaagat ctacgttttt taaagtctct tcatttataa
    10021 attgcgccgg aacagtatta aaaatgaagc gaaaatctcc ggcgtgatcg gaaagtgatt
    10081 ttaaggggaa acttttgtcg gcaataactg acgcgcgggc ggattcttcg gcgttatctg
    10141 taaaaacgga aacgtttgcg gataatccct ttaaacggta aacaatagag tgaccgcatt
    10201 ttccgaatcc aagtactgcg catctgcttt tgtgcagatt gaaaggactg tggaggatcg
    10261 cttcgcagat tgcgccttct gcagtcgaga tactgttgta aacaggaagc gatatatcgt
    10321 ctgcataatc aaaaacagcg acgttatttt cggcggcttc ttttttaaaa gaaggaggga
    10381 tgcccccggc aaacaaagac tgaccgggcc gcagaagatg aagcagcgtc tgaatagaga
    10441 aacgatagat gctattttca accgaaacat ccgcgccgtt tttagtaaac ggaaaagggc
    10501 agatgacgga cccggaatgg atacaggctt cttcgagaga aggcgcttcg cgtactatgg
    10561 aaacatgttc ggagacggag ctgcagttgt tcggctgaga ggccaaagcg taatgacaaa
    10621 gagaacggcc cgattctgct aacatttctg caagaaaaac ctgacgcgca tcaccgccta
    10681 aaacagagaa atcatatgaa aatgaagaca taaaaacacc gcttaagaaa catttttaat
    10741 tactatatgc gagtgagaaa aagatgtgag tataaataac cttgcttgtc gtatattttt
    10801 tcggaatatt cccgggatgt cggcagtgct gtgtatatca gcatcctttt tatagcgggg
    10861 caaaaaatga taaaagagag tgatattaac tatatcacaa aacgaataga aaacaaatca
    10921 cttttgttat tgacatgaaa tcaaataaag tagaagggaa ggcaataaga tgagcagaaa
    10981 aaggaataag gtaatgaaaa acagctccgg ttgtcatgat attaataaag ttataagatt
    11041 ttttcttatt ccttttaaaa aatatgcgtt atcatgttaa tattaggaga gagtaggaga
    11101 ggtgattgat aatgtacaat atattagtat gtgacgatga caaagaaata gtggaagcaa
    11161 tcgaaattta tctgacgcag gaaggatatc gtatactgaa ggcgtatgac ggtacggaag
    11221 ctcttgaaat gcttgaaaga gaagaggttc atctgcttct tcttgatgtg atgatgccga
    11281 aacttgacgg gattcgggca actttgaaaa tacgagagaa aaacagtatt cccatcatta
    11341 tcctttccgc aaaatcggaa gatgcggata agatactcgg acttaatgta ggagcggacg
    11401 attatgttac aaagccgttt agtcctttgg aacttgttgc gcgagtgaaa tctcagcttc
    11461 gcaggtatac gcagcttggc ggcacggctc agaatgaaaa tgataaaata tattctgtcg
    11521 gcggccttag gattaatgac gatctgaaag aagtgacagt agatggggag ttagtaagat
    11581 tgactccgat cgaatataat atcctccttc ttcttatgaa gaatcagggg cgcgtttttt
    11641 cgatcaatca gatatatgaa atgatctgga atgaggaagc tatcggggct gacaatacgg
    11701 ttgccgttca tatacggcat ataagggaga agattgagat taatccaaaa gagccgcggt
    11761 atttgaaagt tgtctgggga gtcggatata agattgagaa ggtgcagtag ataagaaaaa
    11821 caggtaaaga gaggaatgtg aatgaaagga tggtacagaa ggactgcgat gaaagcggtc
    11881 gtcctgatcg taggcgtgtt aaccggagcg atgtttttca catctcttat agtaacaacg
    11941 cggctgaccg gaacgctgaa tccggcagag acggtaaagt taataggaga gccatatgaa
    12001 gaatcttcgg attttagcaa tacagtagaa agctatatgc ttcaggtgct tgaacagttt
    12061 cagttaaaaa cgctgtttga gtcagacgga gcatacaatc cggataaagt gatcgacgta
    12121 atgtcttatt ttggagcgtc gggagcagac gctgaaaatc attccggact ggtatacaga
    12181 ctgtcggatc tgatagagtg gagcaaggca tacagtgtag aatcaggcgg tgtatatgat
    12241 aaaaacgcag taatcgtctg caaacagcca agcggaaaat actattatta ctatacggaa
    12301 gagtttctga atcagcttga aacaggagaa cttcaactcg tattagaaga tgatagaatg
    12361 gatgttgagt catttttaaa tgacttaagc aacggaacgc ttacctcatc cggagtgagt
    12421 gataaaataa gcatatatga tgcagaaggc agtaaattgt atacagactg ttggaattac
    12481 ggggattcag tccgtgagaa ttatgcgccc ttaggagcgg ctaatcttct ccaagttgta
    12541 aatgaacgcc cggagttaaa cggaaagctt atggttattt atgatcatct ggcatctgtt
    12601 cttacgagta taaatgataa atacagccga tataagagcg ggtgggaaca cctggaagag
    12661 ggaaacacaa actttactta tttatatata gatcaggata caaaaaaagt tacgacaaat
    12721 aaaagcgaat atgcaaatct tatggcaaaa ggcgagtcgg gaataaaaac gaacattgaa
    12781 gaatgcatag cggacatgaa atcaggaaaa aatgtgaaat acatttttgt ttatccgaaa
    12841 ttaaaagatt ttgaaacaaa tatggatata ttaccgacaa atgaatggga tatggtgcgc
    12901 tcttatgata ccgacaagac gtatgacagt atttttgcgg tggcagttga tacttcttat
    12961 ccggtcaaag atatgttcta tgaaagagac cggatttatg aaaaaaatat cccgctgcta
    13021 agaggcgggt tcgtgatctt gtgtcttagc ggaattttgt tccttatcag ttttgtcatg
    13081 tcagggttga tggcaggaag aaaagatgga gataacgaag ttcatttaac aggctttgac
    13141 agatggaaaa cagagatcgc ggcagttgcc gtgatcggag tatgggcagt aggaacggca
    13201 gttatcgtag gcatgggaat gatcgacagc gataatgtct acaatatggt agaaactctt
    13261 gactattatg gagcgcgcta tatgagcgat atgccggaag tttattctat gctgtttgtt
    13321 cagaaagtaa gttttttaga gatagcgggt gcatttttat atggagcgtt tacgtttatc
    13381 tgctttttcg cgggttatat gagtcttatc cgcagaatga aagcgaagag tttgtggaat
    13441 ggaagtctgc tttgtatgat tctttctttt tgcaaaagaa tagtcgattc gagtaatgac
    13501 gtttggcgga ccgttctttg gatcagtgca tttttagtca tccactggat tacgtttttt
    13561 gtcagaaatg taccgatggc gctggcagct ctggcagcag acggagtgac gttttggatt
    13621 gtcgtgggaa gtatgcttgc aaaagtcagg atcaagaaag ggatcgaaca gatcgcatca
    13681 ggcaatcttg agtatcggat cgaactaaaa ggactgcggg gaacggaacg caatatcgcc
    13741 gagaatgtca atgatatcgg aagcggtctg aaccgcgtga tcgatgaggc gatgcgaaac
    13801 gaacgtttga aaacggattt gatcacaaat gtatcgcatg acatcaagac tccgcttaca
    13861 tcaattataa attatgtgga tattttgaag cggtctgata ttgccgatga aaagatacta
    13921 gggtatcttg atattcttga agtaaaagcg cagagactta agacgctgac agaagacgtt
    13981 gtggaagcgt cgaaagtaag cagcggaaat attacgttag aatatatgga tattgatttc
    14041 cgtgagatgg tacagcagac ggaaggggaa atggcagaaa aatttgccgc gcgaaacctg
    14101 tcggttgtat tgaaccttcc ggaagaagcg gcggtgatac atgtagacgg acgccgtatg
    14161 tggagggtgc ttgaaaatat cttcggcaat gcggcaaaat atgcaatgcc ggggacgaga
    14221 gtttatgcag atctttcggt atcggagtcg gaagttaagt tttctttgaa aaatgtatcc
    14281 gagcagcagt tgaatatttc ggcagatgag ttgacagagc gattcatcag aggagatatt
    14341 tcccggagta cggaaggaag cggactcggt ctttcaatcg ccaaaagtct gactgaaatg
    14401 caaggaggag ttttcgagtt gtatcttgac ggagatctgt tcaaagtaga catcagattt
    14461 ccaaggataa agccgatgta aaatatatat tgattggatt cgccccttcc ctttttaagg
    14521 taggtttttc accttaggga aggggattgt gtatcctttt tcttacgaag aaatagcaga
    14581 ttacctgcag tatgatcgtg ataagccatg atgcgggata agagataaac aaaacatcca
    14641 atgatctgtg gcgagggaag atcatgaaga tccagacaat cctcgttccg actgttccga
    14701 tgatagaaag aagcatcgga attgcagaat atcccattcc gcgcaacgcg ccgggaaaga
    14761 ggtccatcag tccgcacata aaataagtta cggtagtata caaaagtatt tccattccgc
    14821 attgaatgac tttcggatct gaagtataga tttttaacag ttccggtccg aagagatatg
    14881 aacctccgcc taagattaat gaaacgacaa cggacagcag aatacagttc cgaagaactt
    14941 tgtccattcg tttccatttt cccgcgccgt agttctggct tgtaaaactc atacatgcct
    15001 gtgtgatcga gttggctgcg gcgtacagaa atccgaagat attatttgca gctgtatagc
    15061 ccgccattgc gatagaacca aaagagttta ccgaagattg aagaagtacg tttgagaagt
    15121 tgataaccgt actttgtatg cctgcgggaa tacctacctg aaaaatttgt tttaaataga
    15181 tccacttgat tgtcattgcg gaaaatcgaa gttgatagct tccgtccgag cgatatagac
    15241 agcgaaggac aaggagacag gagatcatct gtgcgatcac tgtggcgatt gcaactcccg
    15301 cgactccgag gtgtaatacg atcacgagga agaggtttaa agctgcgttt acgattcccg
    15361 cgatgataag gaaaaataaa ggccgttttg tatctccgac ggcacgcagg attgccgctc
    15421 cgtaattata gagcataaag aagggcattc cggcgaaata aatccgcata taaagggcgg
    15481 actggccgat gacgtctgaa ggagtgtcca taagcagcag ggctgctttt gaaaagaaaa
    15541 gtccgacaaa gaccatgacg attccgctga tcaaagcgaa agtaactgcg gtatgtactg
    15601 tttcagacat ctgcttttcc tgttttgtcg catagaaacg ggcagccagt acgttcgctc
    15661 cgagggaaac tccgataaaa agattcgtaa atacattgat tagcgctgtc gtagagccga
    15721 ctgccgccag agcgcggctt cctgaaaatc ttccgacaac gacgatatct actgcattga
    15781 aaagaagctg gagaatactg gagagcatga gcggaatgga aaatgaaatc agtttgtcta
    15841 aaacagttcc attgcacatg tctatttcat atttgttctt tttcgatttt gaagctgtca
    15901 ttttaaatcc cctctttata aagtataagc tttgttaaaa aactattacg gtttcattac
    15961 agattatatt atgaatagaa aacgggaaat tttcaagatg aagtttttaa taacttttcg
    16021 agaaattttc atgagtaaaa caatggaatt agccacgata agcacgcagc gcggttttac
    16081 gaatggcgcg ggctgaagtg ttattttgta aatgcatttg taaaagagag gtatttatcc
    16141 aacctttaca ttatttggta tgtatgctat cctttttaaa gacaaaacca actgaagatt
    16201 cagaggagat atttcttcgg atcacagggg gataaaggag agaaaactat gtcagagaaa
    16261 cagaaatatt atattacgac ggcgattgcc tatacttccg gcaagccgca tatcggaaac
    16321 acatatgaga tcgttttggc agatgcgatt gcccgttata agagaagtca ggggtatgat
    16381 gtattttttc agacaggaac agatgagcat ggtcagaaga tcgaactgaa agcagaagaa
    16441 gcgggcgtca caccgaaaga atttgtagac ggtgtttccg ctgagatcaa gaagatatgg
    16501 gatctgatgg atacgtccta tgacaaattc attcgtacga cagacgaaga ccatgagaag
    16561 caggtacaga agattttcaa aaagctgtat gatcagggcg atatttataa aggtcattac
    16621 gaaggaatgt actgtacgcc gtgcgagtct ttctttacag agtcacagct cgtagacgga
    16681 aaatgtcctg actgcggaag agaagtgcaa ccggcgaaag aagaagcgta tttcttcaaa
    16741 atgagtaagt atgcagaccg actgatcgag catattaata cacatcctga atttattcag
    16801 cctgtttcca gaaaaaatga gatgatgaac aacttccttc ttccgggact tcaggatctg
    16861 tgcgtatcca gaacttcgtt taagtgggga attccggttg attttgacga taagcatgtc
    16921 gtttatgtat ggcttgacgc gctgacaaac tatatcacgg gaatcggtta tgatgcagac
    16981 ggagacagca cggaacagta tgcaaaattg tggccggctg acctgcattt gatcggaaaa
    17041 gacattatcc gtttccatac gatctactgg ccgatctttt taatggcgct tgggcttccg
    17101 cttccgaagc aagtattcgg tcatccgtgg cttcttcagg gagacgggaa gatgagcaaa
    17161 tcaaaaggaa atgttcttta tgcagacgag cttgttgatt tcttcggggt agatgcagta
    17221 cgttatttcg ttcttcatga gatgccgttt gaaaatgacg gagtgatcac atgggagctt
    17281 atggtagaac gtttaaattc cgatcttgcc aatacattag gaaaccttgt aaaccgtaca
    17341 gtttctatga caaataaata ctttggcggc gttgtttccg ataaaggagc ggcggaggat
    17401 gtcgatgcag atctgaaagc ggtgatcgac aatacaccga aagcggttga tgcgaaaatg
    17461 gacgggctgc gtgtggcgga tgcgatcaca gagatcttta atctgttcaa acgctgcaac
    17521 aaatatatcg atgaaacgat gccgtgggcg ttggcaaaag atgaagaaaa gaaagatcgt
    17581 ctggaaacag ttctttggaa tctgatccag ggaatctcag caggagcgcg ccttcttgaa
    17641 tccttcatgc cgtctacaag caaaaagatt ctggatcagc tcggagacgg gcatgtgacg
    17701 gagaaaccgg agattctttt ccaaagactg gatctggaag aagtaatgaa aaaagtagaa
    17761 gaacttcatc ctcatataga agaagcagag gaagaggaag atgtgatcga tatcgaggca
    17821 aagccggaga ttacgtttga acagtttgga gcgatgcagt tccaagtagg agagatcatc
    17881 gcttgtgagg cagtgaaaaa atcaaaaaaa ctcctttgtt ctcaggtaaa ggtaggaagt
    17941 caggtgaaac agatcgtttc aggtattaaa gcacattata caccggaaga gatggtaggg
    18001 aaaaaagtga tggtacttgt gaacctgaag cctgcaaagc tggcgggagt actcagtgaa
    18061 ggcatgcttc tttgtgcaga agatgcagaa ggcaatctgg cgcttatgac tcctgagaaa
    18121 gcaatgcctg cgggagcgga gatctgctaa taagaataga gaagaataga taagattaga
    18181 taagattaga taagatcaga gaaacataga gaaaaaaata tctgttggct gaacacagac
    18241 agttcatccc gcagatattt ttttattcta tagaaaattt tgagttctta tgggataaca
    18301 tctttcggga agatattgaa gtgggattgt cactttgttc ttacagagaa aagttaaaaa
    18361 attttgttgt ttttaatgca acaaaatgct tatcaaggta ctcttatata tgaaagcata
    18421 tttaagtcag tgcgaaagag atgaaaggag ggagcttatg tccgcaggaa tagtggatat
    18481 acagcgtcag cttattaaaa aagcgaagtt gggagatacg aaagcctttt cgcagttgta
    18541 tgctgagatt tataaagatc tgtatcggtt tgcgctttat atgacgaaac atacgcagga
    18601 tgcagaagat gcggtcagtg aggcagtgat atctgcatat gaaaatatat ccggactaaa
    18661 gaaagaaaat tctttcaaga gttggatgtt tacgattcta aataatgagt gtaaaaagat
    18721 attacggaaa aacggtaata cgtcgaatca agtgagtgaa gaggatgcca aaaacctcgg
    18781 agcagaggaa atagattttg cacagcgcca tgatgtaaag aaggcgtttg aatcattaga
    18841 ggaagaggag cagacgatag ttgcattttc cgtattcgga ggatacagaa gcgaagaaat
    18901 agccgagatg ctggggaaga acccgggaac ggtccgatca aggaagagcc gtgctttcgg
    18961 caagatgcgt gaaatgctgg actgaatggc aggcgttttg aagatggaga aaatagaaga
    19021 taataagaag aaaaaataac aaaaaccatc aaaataacga aaggagatta tctatgtcag
    19081 agaataaaaa agaaacgaaa aatcaggata tgattgagaa agaaatgaaa gagaagatga
    19141 agcaaactgt tgaagatgaa aagatccccg gttcgcttga gccggaagcg gttgaaaaga
    19201 tgttggagac gagaaagaag aaaaagtcga ataatcgccg ttggaaatat gcctcagtgg
    19261 cagcagcggc ttgcgtttgt cttgtagtgg gagcagtcgg atatacgaat atgaacgcag
    19321 gaaaaaatga acggccggga acggtaacag aaggcagatc agaagaagat cagggaaaaa
    19381 tcgcactggc aaaaaattat gatgaaatta aaaaatattt gaaagaaaat gagaaaaggg
    19441 cgaaagggca gaacggattc ggcagagcag acgcggccta tatgacggac ggggccatgg
    19501 cggaatcttc tgtagccgaa tcgacaaaag atatggcagg tgccgattat tctgatacaa
    19561 atatcagaca agagggagtc ggcgaagcgg acactgttaa gactgacggg aagaatcttt
    19621 acattctaaa tgatcagaca gtagagattg tagatgtatc tcaagcagag atgaaagatc
    19681 tggcaagtat cgagatagat gaaaattgtt ttataagaga ggtttttgtt tccgatgggc
    19741 ggcttgtgat attgtatacc gaatcaaaat gtgaagaagg aaagaaagca gaagacggaa
    19801 tttataaaga ttatacatgc gcggcagttt atgatgtgac cgatccggcg aatccgagag
    19861 aagcaggagt gatttctcag agcgggcagt atcatacaat gcgcgtgaaa gacggatatg
    19921 tttatttagt cagcgatttt tacacatatt atgattcgtc ggtatcaaac gaaagcgatt
    19981 atatcccaca gatccaggga agtctgctcc gcgcggaaga tatatatatg ccgcagggaa
    20041 cgaccggaag tcagtacacg gttatttctg catttgcgct ttccgacccg actgagaaat
    20101 tacagacaaa ggcgattttc ggaaacgcgg gaatgtgtta tgtgagcgaa aataatattt
    20161 atataacaga ggagtattac ggaaaaagcg agacagagaa tatccagact tcgatccgta
    20221 agatcgctta tgataaggga actttagatg cggtaggaca gacaaagatc gacggcgttt
    20281 tgaatgattc ctttagtatc gatgaatata acggatattt gcgaattgca gcgacagtga
    20341 ttcccagtga ttataacaac cggatcatgc ctgttccgta tgtggaagaa ggcggatcgg
    20401 atgttatagt tgaagatgaa gtggcagtgg ataatgcatc tatagaaaca aatgcgttat
    20461 atgtgttgga tgaaaatctg gaaatgaccg gaagtattca gaatttggcg aaagaggaaa
    20521 caatccattc agcgagattt atgggagata tcggatattt tgtgacattt aagcagatcg
    20581 atccgctgtt ttccgtggac ctttcagatc cgtcaaatcc aaagatcatc ggagaattga
    20641 agataccggg attttcagat tatctgcatc cgtatggcga tggaaaactg cttggaatcg
    20701 gattgagcgt agatgaagag ggcatgacga cagaaggtgt gaagctgtct atgtttgaca
    20761 taagtgatcc ggcaaatgta aaggagatgt caacctatgt tttagagaat gtgcttagca
    20821 cagacgcagg atataattat aaggcggtat ttgccgatgc agaaaagaat ctgtttggat
    20881 ttatggcgta cggagattcg atcgaatata aaatgtttac ttacgatgaa gcggaaggtt
    20941 ttaaagaagt attttcaaaa gatattgtta attacggaaa tgtccgagga ttgtatatcg
    21001 gagaaacatt ttatcttgta gccggaaata cagtcgaatc atttacgtta aacggatttg
    21061 aaaaaataga cgatcttgtc ctgtaataga cgattggcag atggaatgga tttc
//
LOCUS       DBMDXF010000006        21106 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_790_length_21106_cov_2.435515, whole genome shotgun sequence.
ACCESSION   DBMDXF010000006 DBMDXF010000000
VERSION     DBMDXF010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 21106)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21106)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21106
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_21106_cov_2.435515"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..680
                     /locus_tag="ACZPD4_00530"
     CDS             3..680
                     /locus_tag="ACZPD4_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1377597.1"
                     /translation="MENIKVLVVDDESRMRKLVKDFLTREGYAVLEAGDGMEAMDIFY
                     EDKEIALVILDVMMPKMDGWQVCREIRETSKVPIIMLTARSEERDELQGFELGVDEYI
                     SKPFSPKILVARVNAILRRTLGNTAGDVIEAGDIKIDKAAHIVKINDKMIDLSYKEFE
                     LLSYFVENQGIALSREKILNNVWNYDYFGDARTIDTHVKKLRSKLGDRGEYIKTIWGM
                     GYKFEVN"
     gene            698..2179
                     /locus_tag="ACZPD4_00535"
     CDS             698..2179
                     /locus_tag="ACZPD4_00535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1377598.1"
                     /translation="MIKYSIRRQMTAIFIGLFSLILGLVLIINNAFLGHYYLSHKADD
                     LIRTYNDVNAKLESGSLSDSDSVRDILTQLERANVDLVVVDGGGNAILTTLGESDPRL
                     KEMFSGLFGMEGVDHENILKRNEDYTIYRDKSSQVQRDVEYLKMWGRFMDGSWFIMQC
                     PYASITESASLANRFLIYLGCIGMVIGGLLVWMFSRSITRPIMELAGLSQEMANLNFD
                     TKYTRGGMDEIGVLGNNFNKMSEKLERTVSELKQANNQLQKDIEQKEKIEDMRNEFLG
                     NVSHELKTPIALIQGYAEGLKEGISEDPESREFYCDVIIDEAAKMNVMVKNLLTLNQL
                     EFGSDEVQFERFDIVRLVKGVIASCEILVQQAGAKISFISDESICVWADEFKTEQVVR
                     NYLTNAIHHVGNEKRIEVRIENGDGKVRITVFNSGTPIPEEDIPKLWDKFYKVDKAHT
                     REYGGNGIGLSIVKAIMESFHQKYGVENFDNGVAFWFELDAKA"
     gene            2368..3774
                     /locus_tag="ACZPD4_00540"
     CDS             2368..3774
                     /locus_tag="ACZPD4_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1377599.1"
                     /translation="MYDKIYEIIQSADDFVWGWGMIALLLGTHLFLTVRTGFIQRKTI
                     TKGIRLSVAKEEGADGEVSQFGALATALASTIGTGNIIGVGTAIALGGPGAVFWCWIT
                     GIFGIATKYAESLIAVKYRVKTEDGRMQGGAMYALERGLNMRWLGLAFAFLAGFASFG
                     IGCATQVNAIAEVCSKNLGIDPFVVGVIVAGMTAVVIFGGIKSIAKVCEKLVPFMAAF
                     YVIGCVIILFINYDYIVPAITTICRLAFTKGAAAGGLVGGGIRYAIQYGVARGLFSNE
                     SGLGSAPIAAAAAKTKNPVRQALVSSTGTFWDTVVVCLMTGLVLVTTIMKNPAINIDE
                     AINGGELTTMAFGQIPFFGSLILVVGIISFAYSTILGWAYYGERCVEYFAGKAGLLPY
                     RLLYVAVLLVAPVLALDLVWKVADILNALMAIPNLTAVLLLSPVIAKETKKYIKNLDA
                     VDHTPVPVVKTGRGKARK"
     gene            3868..4473
                     /gene="hisH"
                     /locus_tag="ACZPD4_00545"
     CDS             3868..4473
                     /gene="hisH"
                     /locus_tag="ACZPD4_00545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1377600.1"
                     /translation="MIAIIDYDAGNIKSVEKALLSLGQDVTVTSKRDEILGADKIVLP
                     GVGAFGDAMENLKKRELDVVIREAAESGIPFLGICLGLQVLFEKSEEAPGVEGLGILK
                     GEICRIPKTEGLKIPHMGWNSLHLENDGRLFRGIADNPYVYFVHSYYLKAEDERIVKA
                     SAEYGTHIHASVEQGNVFACQFHPEKSSTVGLQILKNFVEL"
     gene            4486..5247
                     /gene="hisF"
                     /locus_tag="ACZPD4_00550"
     CDS             4486..5247
                     /gene="hisF"
                     /locus_tag="ACZPD4_00550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1377601.1"
                     /translation="MFTKRIIPCLDVKDGRVVKGVNFVDLKDAGDPVEIAKAYDAAGA
                     DELIFLDITASSDQRGTVVDMVREVAKCVFIPFTVGGGIRTVDDFRALLREGADKISI
                     NSSAINTPELIRDAADKFGSQCVVVAIDAKKRQDGSGWNIYKNGGRVDVGIDAVEWAQ
                     KVEQLGAGEILLTSMDCDGTKSGYDLELTKAIAQSVSIPVIASGGAGKLEHFKDALAE
                     GMADAALAASLFHYKELEIRQVKEYLAKEGVSVRL"
     gene            5340..6008
                     /gene="ung"
                     /locus_tag="ACZPD4_00555"
     CDS             5340..6008
                     /gene="ung"
                     /locus_tag="ACZPD4_00555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1377602.1"
                     /translation="MSGISGDWYEALKGEFSKPYYRTLFQTVNEEYRTRLIFPPAEDI
                     FNAFHLTPLKKVKVVILGQDPYHNNGQAHGLCFSVKKGVDVPPSLVNIYKELHDDLGC
                     TIPDHGCLTKWANQGVLMLNTVLTVRAHQANSHRGIGWEEFTDAAIMALNGQDRPIVF
                     ILWGAPAQRKKAMLNNPKHLILTAPHPSPLSAYRGFFGSRPFSKTNEFLESQGKEPID
                     WQIE"
     gene            6244..6987
                     /locus_tag="ACZPD4_00560"
     CDS             6244..6987
                     /locus_tag="ACZPD4_00560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377603.1"
                     /translation="MSKESKKEETVREIISVKNLYKLYRVGDSVVRALDGVSFSVYEG
                     EFCAIVGTSGSGKSTLLNMLAGLEKPTRGEVIVAGKHIEQMKENELVKFRGENVGFIF
                     QSFHLMGTMNALENVALPLTFRGEPRKERIKKADRMLDLVSLEKHKKHLPNQLSGGQQ
                     QRVSVARALVNDPKIIFADEPTGALDSHTSQDVMRLMQRLVREQKRTLVMVTHDRSQA
                     EYADRVLEIIDGKIVHITENRRKEENERA"
     gene            6974..8602
                     /locus_tag="ACZPD4_00565"
     CDS             6974..8602
                     /locus_tag="ACZPD4_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 S-layer family protein"
                     /protein_id="HIR1377604.1"
                     /translation="MREHRKMTLKRIGIGILCMVFVLSMLAPLVAFAEEKTESGSQVS
                     KRELAYIQISVGDGQETPVFKAGQKVSEFRINVKNNGNVDAHNVRIEPDIKNASDWPF
                     ELDKLNYEKELSTIKSGGQTAAVWGSEEKPLIVRGDVTGKSYKLEFKISYDDGEKSYE
                     TDKYVFVKTTAKEKPSENQGGNANGGEINNGNTGNGSGGSGNAQGTPDDSGAVFGGVY
                     TSDPVMAGGSAGGADASSASVPRVIVTGFNTEPAEVRAGSNFKLIVHLKNTSSKTAVS
                     NMLFDMQAPSSGSEGAAEAPAFLPASGSSSVYLDGIPAGGTSDISIELNARADLVKKP
                     YSISMSMKYEDSSAAQYEAQSSLAIPVIQDARFEFSKIEIAPETVAVGEEANISCSLY
                     NLGRVKMYNVKARFEGKMIESDEQFIGNLDSGATGMIDAIVTATEETTDDTECKLILT
                     YEDDAGNVSTAEEKFDLTVMPMSDAGDLDAMADIPEEQGGLPIGMILAAAVVVIVAVA
                     VAIVLIMKRKKAKRLEAEEEELFDEVERSAEDER"
     gene            8568..9989
                     /locus_tag="ACZPD4_00570"
     CDS             8568..9989
                     /locus_tag="ACZPD4_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377605.1"
                     /translation="MRWSDLLRMSVSSLRRRKLRTFLTVLGVVIGTASIVVMISLGLG
                     LQESMYKEVEQNGGTTSITVTGKDSGGMMYGYSGMSSDEEESQKYITDDVIKELKNVA
                     HVKSAEPILSISVIALKGKYEGFIELTGMTPEGLKTLNLELEDGGRLPDPNSAQLELV
                     YGNTVITNFYEKTSGRGYWDSGELPDIDLQTDSVFLILDQDGYYQSQSAASDIGGSAG
                     ESGEGGTASAKTPPKKHVVSASGVLKGGPDTYTVNSYMTFCDINKLRTYLEKEFRGRA
                     IPGQPTTKSGKPYNKFVYSSAKVQADDMENVEAVSDAIRAMGYNVSSNIEYIDSMKKQ
                     FAMVQAVLGGIGAVSLFVAAIGIANTMMMSIYERTKEIGVMKVIGCSLGNIRQMFLLE
                     AAFIGLGGGVIGNVLSFLMSAAINALVKGKEMGMTTGQISYIPLWLVLVSLGFAMLVG
                     TAAGYFPARRAMKLSPLAAIRNE"
     gene            complement(10099..10362)
                     /locus_tag="ACZPD4_00575"
     CDS             complement(10099..10362)
                     /locus_tag="ACZPD4_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377606.1"
                     /translation="MFRLWGKEFKENRLLRDTVICDETTDTRTHKIFNALDAICYEFD
                     LSKPIWLDTTIADFKRHAKARFYQDNFVDSIDFDYLEIEVIEE"
     gene            10567..11031
                     /locus_tag="ACZPD4_00580"
     CDS             10567..11031
                     /locus_tag="ACZPD4_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377607.1"
                     /translation="MIRVCTINDKEILEKYLQEEPYAGAILAAIEEFGFDEKFQTVYL
                     DSEKQNLDTEGEQETEETVKGVYLWFHKNLLLYSKENKVDIDFLEQMIFMAAPDCVVG
                     RKDNVNIVSWLLTDYHFKQSDMIPEIVDAEGKTTPCFAAKEAYAGEWGYLKK"
     gene            11028..12083
                     /locus_tag="ACZPD4_00585"
     CDS             11028..12083
                     /locus_tag="ACZPD4_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIR1377608.1"
                     /translation="MKKLLDRIFIDGLGGMAQGLFATLIIGTIIQQIGLFMGGGTGDL
                     IYELGKIAAGLTGAGIGVGVARKLEAGHLVIVSAAVVGMIGAFAGKILSGQVLVDGNL
                     VLAGPGEPLGAFAAAYLAIEIGILISGKTKLDIILTPLVCIGTGSAVGLFVGPPISEF
                     MLWLGSLINWGTEQQPFLMGIVVSVLMGMILTLPISSAALGVILNLSGLAAGAATIGC
                     CCNMIGFAVASWRENKFSGFLAQGIGTSMLQIPNIVRRPLIWLPVILSSAVLGPIGTM
                     LLHMTNNATGSGMGTAGLVGPLMTWQVMTQSEAGVIVLGKIILIQFILPAAVTLLVSE
                     FMRKKKWIRQGDMKLEL"
     gene            12174..12893
                     /locus_tag="ACZPD4_00590"
     CDS             12174..12893
                     /locus_tag="ACZPD4_00590"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1377609.1"
                     /translation="MTSEQQVEAALKSYEDVDSWKTIIFLYNAALKEVGTKLEILNDE
                     FQHVHQYNPIEHIKTRIKTPESIVKKLRRYGHETSIENMVRYINDIAGVRLICSFTSD
                     IYRLAEMIGNQSDLKVLSIKDYIKNPKESGYKSYHMLVSVPIFLSDSVVDTKVEIQIR
                     TIAMDFWASLEHKIYYKFEGNAPDYISRELKECAKMVSELDDKMLSLNEAIQACMIRD
                     DQRKESMEDVFDNVIGERRKQ"
     gene            12994..13359
                     /locus_tag="ACZPD4_00595"
     CDS             12994..13359
                     /locus_tag="ACZPD4_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1377610.1"
                     /translation="MQEEKTEIERCDFIHVHEDVVKKVKEHIPAERELNDLAEFFKVF
                     GDVTRLKLLSVLFHSEMCVCDLAKTLNMTQSAISHQLRILKQMKLVKNRREGKTVFYS
                     LADGHIKTIINQGMEHIQE"
     gene            13481..15346
                     /locus_tag="ACZPD4_00600"
     CDS             13481..15346
                     /locus_tag="ACZPD4_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1377611.1"
                     /translation="MTRETRKRELEIGIGLVLYLLAFLISMQAKMKSKAEMLLFLLPY
                     IVSAISIYKEQAEKFLKKQFLDENLLMIIATVGAFFVGRYKEAVAAMLFYQVGKLVEE
                     ISLSRTKKSIAKFIDIRPEYANLKIGNTEKIVPPQELNLRQTIVLHPGDKIPVDAVVT
                     SGTGTVDMKALTGEPDPVTVRIGDRLFSGSINLNSVLEARVEKLYNDSTAAKIMRLVE
                     SANENKSESVHFVDKFTKYYTPIVILIALLTVILPPMMFVEAKAEWIYRGLIILVAAC
                     PCGLMVSIPLAFLGGIGSASKQGVLIKSGAVLEDLIRVDTYVFDKTGTLTEGVFHVKD
                     IVPYKFEKERLLELAALAESYSKHPVALSLAEAYGKPAESTRIKDVKEEPGYGVHAFV
                     DGLEVYAGNTRLMNREGILYAQTEKDGTAVHVAVDGKYAGYILIADTIRKDTGKLMRW
                     MRKKNMGTVMLTGDNERTAEKVAAELKIDSVYAELMPEDKVQLLEEFCNDRMENEKLA
                     FVGDGMNDAPVLALADIGIAMGGLGADAALEAADIILMEDEPSKIINAVRIAKGTIRS
                     VRENVLFAIGMKVFLVILAFFGLVTMQSAIIADMVVMVINILNSFWMMKYPESGV"
     gene            15354..15500
                     /locus_tag="ACZPD4_00605"
     CDS             15354..15500
                     /locus_tag="ACZPD4_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377612.1"
                     /translation="MKIKKQIVLAAVLCMAVPTVASGCANSAKSGVEALEAGDYKEAQ
                     AQKN"
     gene            15551..15934
                     /locus_tag="ACZPD4_00610"
     CDS             15551..15934
                     /locus_tag="ACZPD4_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1377613.1"
                     /translation="MTYYEQEEYSSALDAFKKAVDTGVVQTTQIYNLMGICAMKTEDY
                     EAALEYIQAGLAMAETDMSGEEEKNSENGKDSAEMIQEMRYNEVVCYEKLADWENAKQ
                     KASEYLIEYPEDTAMEREAEFLETR"
     assembly_gap    16117..16216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16324..17565
                     /gene="hflX"
                     /locus_tag="ACZPD4_00615"
     CDS             16324..17565
                     /gene="hflX"
                     /locus_tag="ACZPD4_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1377614.1"
                     /translation="MYEMEQIDERVILVGIDTGNEDAANRSLDELSELAKTAKAAVVG
                     RLIQPRESAHPGTYIGKGKLTELKDLIWETNATGIICDDELTSAQLGNLEEELSCKII
                     DRTLLILDIFAARAVSGEGKIQVELAQLRYRASRLAGLGRSLSRLGGGIGTRGPGEKK
                     LEMDRRLIRERISRLKKELKDVEKHRELIRTQRKQSGLKVAALVGYTSAGKSSIENAL
                     TNAGILEDAMLFSTLDTTTRSLVLDNTQEILVTDTVGFIRKLPHHLVEAFKSTLEEAK
                     YADIIIHVVDASNPQMDEQMHVVYDTLRQLGAADRPVITLFNKQDKLESAGSYRDFQA
                     EYSIPASAKTGEGLAELKKALLEIVRREQIYVERLYDFSEASKIQLIRSRGQLLSEKY
                     VPEGIEVKAYVPKDIYGKVCI"
     gene            17868..>19884
                     /locus_tag="ACZPD4_00620"
     CDS             17868..>19884
                     /locus_tag="ACZPD4_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1377615.1"
                     /translation="MKKLTRAGAGALAGVMIFGLAAPLAPLSVEAAGEIENLALNKTA
                     TASEQETDSYSAAKAVDGVVNRDAAKPQSRWATNTHSNQEAQWLKVDLGEKKTFRSFV
                     LAWERRNITSYKIQISDTGEDNDDSKWTTVYEKKAGDKISGINENIRLDEPKEAKYVR
                     LYIDGYTADAVGDSVSWQSVSLYDFQIYADEIPDDVLPNENYCLEGTAEANDYEKTDG
                     DTQAPSKAIDGNLTTRWATNPDNEGKGAADRTLTVTLPVTQRVQHFKINWEKTNIKEY
                     TIEVKDSSTGEFKQVYENTKQITSLEEIISLDAPVWAKEIRLNVKSYGEAVPNWYNVS
                     VAEFGAYAVKPEATPDPETPEDETLQALANSIAAPQFNADKTQLTWKTPEGVKVEFLA
                     DYEQIVGRDGKIYQPLTDTEIKGVYKVTKTAASATDKKEKNYAEATVEHTITIPGKHK
                     EAGVNAKPTVVPELAEWYGGETEGNVTISGKIVVDPNAGDLKAAADALATDYEAELGK
                     KMTVATGSNPAAGDIYFTLDKSDNGLGEEGYVMESDSYVKVTAAHQTGAYWATRSIIQ
                     IAELNEGKLPKGTTRDYPKYEVRGFMLDVARKPVSMETLEDVAKEMAYYKMNALHVHL
                     NDNLIFYEDFATTEEAREKAYTGFRLESDIKKDGNGGKNKADLTNKDM"
     assembly_gap    19885..19894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19895..>21106
                     /locus_tag="ACZPD4_00625"
     CDS             <19895..>21106
                     /locus_tag="ACZPD4_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIR1377616.1"
                     /translation="GAFTKVRPDLMLQKTMEGNAKRAGEQFDLSDEKYADSLAFVQSV
                     WDEYLNDDMFDDSMTLHIGTDEYYGEANCFRKFTDDMIKHLKKKGYKVRMWGSLTNKK
                     ENQPDAYPVDGEGVQLNIWSEGYANPQDMYSKGYELINTLEGNYIVPAAGYYNDYVNS
                     QSVYQNWQPNKYSNVTLPAGSDQVLGGTFAIWNDSIDTRANGISEVDIYDRFVKALPA
                     FASKNWGDGTDKNWSEVKAVSDQLGDAANSNPYHKATADESGKYMEYQFENEKDSSDN
                     KRDLELAGGAKLEKGSLKLTGGESYATTPIDKLATGNTLKFDITLDKEAKAGQILFEA
                     DNLGDNGDYVHDIRIMEDGRLGFRRELYDYYFDYKLPVGEKVHLEIRTDGTKTSLIVD
                     GQELQATGVYRN"
BASE COUNT         6502 a         3530 c         5871 g         5093 t
ORIGIN      
        1 ttgtggaaaa tataaaagtg cttgttgtag atgacgagag caggatgaga aaacttgtga
       61 aagactttct tacccgggaa ggatatgctg tgcttgaggc gggagacggc atggaagcga
      121 tggacatttt ctatgaagat aaggagattg cccttgtgat ccttgatgtg atgatgccga
      181 agatggatgg ctggcaggtt tgccgggaga tcagagagac gtccaaagta ccgatcatca
      241 tgctgacggc aagatcggaa gaacgtgacg aacttcaagg gtttgaactt ggagtggacg
      301 agtatatatc aaagccgttc agcccgaaga ttcttgtggc gcgggtaaac gctatcttaa
      361 gacgaactct cggaaatacg gccggagatg tgatcgaagc gggagatatc aagattgata
      421 aggcggcgca tattgttaag atcaatgaca agatgatcga tttaagttat aaggagtttg
      481 aactgttgtc atattttgtg gaaaatcagg ggattgcgct gtccagggaa aaaatattga
      541 ataatgtgtg gaattatgat tatttcggtg atgcgagaac aatcgatact catgtgaaaa
      601 aactccgtag taaattaggc gacaggggcg agtatatcaa aacgatatgg ggtatgggat
      661 ataaatttga agtcaattaa ccggaggaaa tacaaggatg ataaagtatt cgatcaggcg
      721 tcagatgacg gcgattttta tcggattgtt cagtcttatt ttaggtcttg tccttattat
      781 aaataacgct tttttaggac attattatct gtctcacaaa gcggatgatc tgatccgaac
      841 atacaatgac gtcaatgcga agctggagtc cggaagtctg tcagattctg attcggtgag
      901 ggatattctg acacagcttg agcgggcgaa cgttgacctt gtcgtggtgg acggaggtgg
      961 aaatgcgatc cttacgacac ttggagaaag cgatccgcgt ttgaaagaaa tgttcagcgg
     1021 cttattcgga atggaaggag tcgatcatga aaacatactg aagcggaatg aggactacac
     1081 gatttacaga gataagagca gtcaagtaca aagagatgta gagtatttga aaatgtgggg
     1141 aagatttatg gacggctcat ggtttatcat gcagtgtcct tatgcgagta ttacggaaag
     1201 tgcatctctt gcaaaccgct ttttgatcta tctcggatgt atcggaatgg ttatcggagg
     1261 tctgttagta tggatgtttt cccgaagtat tacaagaccg atcatggaac ttgccggatt
     1321 atcccaggag atggcaaact taaatttcga taccaagtat acgcgcggcg gcatggatga
     1381 gatcggtgtg ctcgggaata atttcaataa aatgtcggag aagctggaaa gaacggtgtc
     1441 agaactaaag caggcgaata atcagctgca gaaagatatc gaacagaaag aaaagatcga
     1501 ggatatgaga aatgaatttc tcggaaacgt gtctcatgaa ctaaagacgc cgatcgccct
     1561 tatccagggg tatgcggaag ggctgaaaga aggaatcagc gaagatcccg aaagccgcga
     1621 attttactgt gatgtgatca tagacgaagc ggcgaaaatg aatgtgatgg tgaaaaatct
     1681 tctgacgctg aatcagttgg aattcggctc tgatgaagta cagtttgagc ggtttgatat
     1741 cgtgcgcctt gtaaaggggg taattgcaag ctgtgagatc cttgttcagc aggcaggggc
     1801 aaagatcagt tttatttccg atgaaagtat ctgtgtgtgg gcggatgagt ttaaaacaga
     1861 gcaggttgta cgaaattatc ttacgaatgc gatccaccat gtcggcaatg agaagcgcat
     1921 agaagtccgt atagaaaatg gggacggaaa agtgcggatc actgtgttta acagcggaac
     1981 tccgattccg gaagaagata ttccgaagtt gtgggataag ttctataaag tggacaaagc
     2041 gcatacaaga gaatatggcg gcaacggaat cggtctgtcg atcgtgaaag cgatcatgga
     2101 atcgttccac cagaaatacg gagtagagaa ctttgataat ggagtagcgt tttggtttga
     2161 attggatgca aaagcgtgaa aaacttataa gttcaatgct ttacgttagc gcgtaaatgt
     2221 gctaaaatgc aactaataat tcaaggatag ttgcgtaaga gggcatacgg catgccggct
     2281 gtcagcggtc ggtatatcgt atgccctttt tgcaatagta aaaaatgtaa aaaagagtgt
     2341 aaaagtaaga aggagggcgg gataagtatg tatgataaaa tatacgagat cattcaatcg
     2401 gcagacgatt tcgtatgggg ctgggggatg atcgcacttt tgctgggaac gcatctgttt
     2461 ttgacagttc gcacaggatt tatccagaga aagacaatta caaaagggat tcggctttcg
     2521 gttgcaaaag aagagggggc agacggagaa gtcagtcagt tcggagcgct tgcaacagcg
     2581 cttgcctcca cgatcggaac gggaaatatc atcggtgtcg gaacagcgat cgctctgggc
     2641 ggtccgggag ctgtattctg gtgctggatc acgggcattt tcggaattgc aacgaagtat
     2701 gccgaatcgc ttattgcggt aaagtacaga gtaaaaacag aagacgggcg aatgcagggc
     2761 ggtgcgatgt atgcgctgga gcgcggactg aatatgaggt ggctcggttt ggcgttcgct
     2821 tttttggcag gttttgcttc ttttggaatc ggatgcgcga ctcaggtaaa cgcgatcgcg
     2881 gaagtgtgca gtaagaacct cggtattgat ccgtttgttg tcggagttat tgtggcaggg
     2941 atgacggcag ttgtaatctt cggaggaatt aaaagtattg cgaaagtatg tgaaaagctt
     3001 gttccgttta tggctgcttt ttatgtgatc ggatgtgtca ttatattgtt tatcaattat
     3061 gattatatcg ttccggcaat tacgacgatc tgccggcttg catttacaaa aggagcggca
     3121 gcaggaggtc ttgtcggcgg cggtatcaga tatgcaatcc agtacggagt tgcgagaggt
     3181 cttttctcaa acgaatcggg acttggttca gcgccgatcg cggctgcggc ggcaaagaca
     3241 aaaaatccgg ttcgtcaggc gcttgtttct tctacaggaa cattttggga tacggttgtt
     3301 gtctgcctga tgacaggact tgtgcttgtg acaacgatta tgaaaaatcc ggcaattaat
     3361 attgatgaag caataaacgg aggcgaactg acgacgatgg cgtttggtca gattccgttt
     3421 ttcggatctc ttatcctcgt tgtcggtatc atttcatttg cctattcaac gattttagga
     3481 tgggcgtatt atggagaacg atgcgtggaa tattttgcag gaaaagcagg gcttcttccg
     3541 tatcggcttc tctatgtggc ggtgcttctt gtagctccgg ttcttgctct tgatctcgtg
     3601 tggaaagttg cggatatatt gaacgcgctg atggcgatac cgaacctgac cgcagtgctg
     3661 cttctttctc cggtcattgc aaaagagacg aagaaatata ttaagaattt ggatgcagtt
     3721 gatcatacgc cagttcctgt tgtgaaaacc ggaagaggaa aagcaagaaa gtagaaacag
     3781 aaaaagaaaa tagaaaaacg cagtgcaaaa caaaaaagct gcggaagata aatatagaat
     3841 gaaaaacaaa aactgggaga ggaaaatatg attgcaatta tcgattatga tgcaggaaat
     3901 attaaaagcg tagagaaagc gctcttaagt ctcggacagg atgtaacggt tacatcgaaa
     3961 cgagatgaga tactgggagc ggataaaatc gttttgcccg gagtcggcgc gtttggagac
     4021 gctatggaaa acttaaaaaa gcgggagctt gacgttgtga tccgtgaagc ggcagaaagc
     4081 gggattccgt ttttaggcat ctgcctcgga cttcaggttc tgtttgaaaa aagcgaagaa
     4141 gcgccgggcg tggaaggact tggtattttg aaaggcgaga tatgcaggat tccgaaaaca
     4201 gaaggattaa aaattccgca tatggggtgg aattctttac atttagaaaa tgacggaaga
     4261 ctgtttcggg gaattgcaga caatccgtat gtttatttcg tacattctta ttatttaaaa
     4321 gcagaagatg aacgaattgt gaaagcttcc gctgaatatg gcacacatat tcatgcatcg
     4381 gtggaacagg gcaatgtttt tgcgtgtcag ttccatccgg aaaagagcag tacagtaggg
     4441 cttcaaatat tgaaaaattt cgtggaatta taagaggaga atgtgatgtt tacgaagaga
     4501 attattcctt gtttggatgt gaaagacgga cgggttgtaa aaggagtcaa ctttgtggat
     4561 cttaaagatg ccggagatcc ggtggagatt gcgaaagcct atgatgccgc aggagcggat
     4621 gagcttatat ttcttgatat tacggcatct tccgatcagc ggggaactgt agtcgatatg
     4681 gtcagggaag tggcaaagtg tgtgtttatt ccgtttactg tcggcggagg aatccgcacg
     4741 gtcgatgatt ttcgggcgct tcttcgcgaa ggggcagata agatttccat taactcttcg
     4801 gcgatcaata cgccggagct gatccgcgat gcggcggata aattcggcag tcagtgcgtt
     4861 gtagtggcga tcgacgcgaa aaaacgtcag gacggttcgg ggtggaatat ttataaaaac
     4921 ggcggtagag tagacgttgg gatcgatgcg gtagagtggg cgcaaaaagt ggaacaactc
     4981 ggcgcgggag aaattttact tacaagcatg gattgtgacg gaacgaagtc gggatacgat
     5041 ctggaactga caaaagcgat cgcgcaaagc gtgtcgattc ctgtgatcgc ctcgggagga
     5101 gcggggaaat tagaacattt taaggatgct cttgcagaag gaatggcaga tgcggctctg
     5161 gcggcgtcgc tgtttcacta taaagaactg gaaatcagac aggtgaaaga gtatttggca
     5221 aaagaaggcg tttctgtaag attgtgatct tagaggaaca aggggaaaga ctgcatgaag
     5281 agacaatacc gataaaagcg gcggagatac aaacggaaag aaagacgagg gtgataaaga
     5341 tgtcaggtat aagcggagac tggtatgaag cgctgaaagg tgaattttca aagccatatt
     5401 acagaacatt atttcagacg gtaaacgagg aatatagaac aagattgatt ttcccgccgg
     5461 cggaggatat atttaatgca tttcatttga ctccgctgaa aaaggtgaaa gtagtgatac
     5521 tcggacaaga cccttatcac aataacggac aggcgcacgg gctttgcttt tcagtgaaaa
     5581 agggagtgga tgtgccgccg tctcttgtca acatttataa agagcttcat gatgatctcg
     5641 ggtgtacgat ccctgatcac ggatgcctta caaaatgggc aaatcaggga gttctcatgt
     5701 tgaatactgt gctgactgtt cgtgctcatc aggcaaattc ccacaggggc atcggctggg
     5761 aagaatttac ggatgcggcg atcatggcgt taaacggtca ggaccgtcca atcgtattta
     5821 ttttatgggg cgctccggcg cagagaaaaa aggcgatgct gaacaatccg aagcatctga
     5881 tattgacagc gccgcacccg agtccgctgt cggcatatcg tggatttttc gggagcagac
     5941 cgtttagtaa aacgaatgag tttttagagt cgcaaggcaa agagcctatt gactggcaga
     6001 tcgaatagaa aagaaagcag aaaggctgca gatgttttgc ggcctttttt gttatagggg
     6061 gaaaacagaa gttttgcgca gatgagattc ggatattaag aaattattca taaaatacat
     6121 agcgaaatct aacaaaacat atgatacgat acaaataaca atgcatcgct gtttcgtaaa
     6181 aaggcagatc tgactgccga agattcgtat gtgttgagac aggagataaa acaaacagac
     6241 agaatgagta aagaaagcaa aaaggaggag acagtgaggg agattatcag tgtgaaaaac
     6301 ctgtataaac tgtatcgtgt cggcgattcg gtcgtccggg cattggacgg cgttagtttt
     6361 tcagtgtacg agggagaatt ttgtgcgatc gtcggaacgt ccggttccgg aaaatcgact
     6421 cttttgaata tgcttgccgg actggaaaaa ccgactcgcg gggaagtgat cgtagcgggg
     6481 aagcatatcg agcagatgaa agaaaatgaa cttgtaaagt tcagaggaga aaacgttggt
     6541 tttatatttc agtcgtttca tctgatggga acgatgaatg cactggaaaa tgtggcgctt
     6601 ccgcttacat ttcgcggaga gccgagaaaa gaacgaataa aaaaagccga cagaatgctg
     6661 gatctggtga gcttggaaaa acataaaaaa catctgccga accagttatc aggaggacag
     6721 cagcaaagag taagcgtggc aagggcgctt gtgaacgatc cgaagattat ttttgcggat
     6781 gaacctacag gagcgcttga ttcacatacg tctcaagatg tgatgcgcct gatgcaaaga
     6841 ctcgtaagag agcagaaaag aacgcttgtc atggtcacgc atgatcgaag tcaggcggaa
     6901 tatgcagacc gggtattgga gattatagac ggaaaaattg tacacattac agaaaacagg
     6961 aggaaagagg aaaatgagag agcatagaaa aatgacgttg aaacggatcg gaatcggaat
     7021 tttatgcatg gtgtttgtgt tgtctatgct tgctccgctc gtggcatttg cagaggaaaa
     7081 aacagagtcc ggaagtcagg tgtcaaagag agagcttgcg tacattcaga tcagtgtcgg
     7141 cgacgggcag gagacgcctg tgtttaaggc ggggcagaag gtatccgagt ttcggatcaa
     7201 tgtaaaaaac aacggaaatg tagacgcgca caacgtgcgg atagaaccgg atatcaagaa
     7261 tgcatccgac tggccctttg aacttgataa gctgaattat gaaaaagagt tgtctacgat
     7321 caaaagcggc ggacagactg cggctgtgtg ggggagtgaa gagaaacctc ttatcgtaag
     7381 aggagatgtg acagggaagt cttataaact ggaatttaag atttcttacg atgacggaga
     7441 gaaatcctat gagacggata agtatgtctt tgtaaaaacg accgcaaaag aaaagccatc
     7501 ggaaaatcag ggtggaaatg caaacggcgg agagataaac aacggaaata ccggaaacgg
     7561 aagcggcgga tcgggaaatg cgcagggaac gccggacgat tcgggcgcgg tgttcggagg
     7621 agtatatacc agcgatccgg tcatggcagg cggctcggca ggcggcgcgg atgcgtcaag
     7681 cgcatctgtt ccgcgggtga ttgtaacagg atttaataca gagccggcgg aagttcgcgc
     7741 gggaagtaat tttaaattga tcgtccacct gaaaaatact tcttcaaaaa ctgctgtttc
     7801 aaatatgctt tttgatatgc aggctccgtc atcaggttca gaaggtgcgg cggaagctcc
     7861 ggcgtttctt ccggcatcgg gttcaagctc tgtttatctt gacggaattc ctgccggagg
     7921 aacaagcgac atttcaatcg aattgaatgc acgggcagat cttgtgaaaa agccgtacag
     7981 tatcagcatg tctatgaagt atgaagacag cagcgcggcg cagtatgagg cgcagtcaag
     8041 ccttgcgatc ccggtcatac aggacgcccg gtttgagttc agtaagatcg agatcgcgcc
     8101 ggagacagta gcggtcggag aagaggcgaa tatttcctgc agtctgtaca atctcggacg
     8161 ggtgaagatg tacaatgtga aagctcgttt tgaaggcaag atgatcgagt cagacgaaca
     8221 gtttatcgga aatcttgatt ccggcgcgac aggcatgatc gacgccatcg tgacggcgac
     8281 agaagagacg acagacgata cggaatgtaa gctgatctta acttatgaag atgatgcggg
     8341 aaatgtatcg acagcggagg aaaaatttga tcttactgtt atgccgatgt cagatgccgg
     8401 agacctggac gctatggcag atataccgga agaacagggg gggctgccga tcggaatgat
     8461 cttggcggca gccgtagttg tgatcgttgc tgtggctgtg gcaatcgtgc ttattatgaa
     8521 gaggaaaaaa gcaaaacgtc tggaagcgga agaggaggag ttgtttgatg aggtggagcg
     8581 atctgctgag gatgagcgtt agcagcctgc ggcgcagaaa attaagaacg tttcttacgg
     8641 tactaggcgt tgtgatcgga acggcttcta tagtagttat gatatcgctc ggactcggac
     8701 ttcaggaatc catgtataaa gaggtggagc aaaacggagg aacgacaagt attaccgtga
     8761 cgggaaaaga ttcgggcgga atgatgtacg gttattcggg aatgtcttcg gacgaagaag
     8821 aatcgcagaa atacatcaca gatgatgtga tcaaagagtt gaaaaacgta gcccatgtga
     8881 aaagtgcaga gccgatctta agcatatcgg taatcgcgct gaaaggaaaa tatgaaggct
     8941 ttatcgaatt gacaggaatg acgcctgaag gtttgaagac attgaatctg gagctggagg
     9001 atggcgggcg gcttcctgat ccgaacagcg cccagctgga acttgtatat ggaaatacag
     9061 taataacgaa tttttatgaa aaaacatcag gaagaggata ttgggattcg ggagaacttc
     9121 ctgatataga tctgcagacg gattccgttt ttcttatact cgatcaggac ggctattatc
     9181 agtcgcagag cgcggcttcg gatatcggcg gctctgccgg agaaagcgga gaaggcggaa
     9241 cagcgtcggc aaaaacgccg ccgaaaaaac atgtcgtatc tgcaagcgga gttttaaaag
     9301 gcggaccgga tacctatacg gtgaattctt atatgacgtt ttgcgatatc aataagctga
     9361 ggacgtatct tgaaaaagaa ttcagaggaa gagcgattcc ggggcagccg acgacaaaga
     9421 gcggaaaacc atataataag tttgtatatt cgtcggcaaa agtacaggcg gacgacatgg
     9481 aaaacgtaga ggctgtctca gacgcgattc gggcgatggg atataatgtg tcgtcgaata
     9541 tcgaatatat cgacagcatg aagaaacagt ttgcaatggt tcaggcggtg cttggcggaa
     9601 tcggagccgt ttcgctgttt gttgcggcaa tcggaatcgc aaatacgatg atgatgtcta
     9661 tttatgagag aacgaaagaa atcggcgtta tgaaagtgat cggatgcagt cttggaaata
     9721 ttcgtcagat gtttcttttg gaagcggcgt ttatcggact tggcggaggt gtgatcggaa
     9781 atgttttaag tttccttatg tctgcggcga tcaatgctct tgtgaagggc aaagagatgg
     9841 gaatgacaac agggcagatt tcttatattc ctttgtggct tgttcttgtt tctcttggat
     9901 ttgcaatgct tgtcggaacg gcggcgggat atttcccggc gagaagggcg atgaagttaa
     9961 gtccgcttgc ggcgatcaga aatgaatgac aaccggaaac aggcggcggt tagaaataag
    10021 tgacgatcag ccgatgataa gaggcgcaga tagatcggat aataacgaaa aatggtaaaa
    10081 gacagaggtg accggggatt attcctcgat cacctctatt tctaaataat caaaatcaat
    10141 gctgtctaca aagttatcct gataaaaccg agcttttgcg tgccgtttga agtcggcgat
    10201 cgttgtgtca agccagatag gcttgcttag atcgaactca tagcagatgg cgtccaaagc
    10261 gttgaaaatc ttatgtgtgc gggtgtctgt tgtctcatcg caaatgacag tatcgcgcag
    10321 aagcctgttt tctttaaatt cttttcccca taaacgaaac ataacgaagc tcctttcaaa
    10381 gtttatgaaa tttatcatac aaaaatcccg ttgcttgtgc aagccctttt tgatatatta
    10441 gtatcagttc gggcgctcgg acaaaatata catgcaggga aagccggcaa gtttatatcg
    10501 tgtctgtgcg gtgaaatgaa aaaagaaaac atgatgtgac gcaggagttt gtaaaggagt
    10561 aaaaagatga tcagagtatg cacgataaat gacaaagaaa tattggaaaa gtatttacag
    10621 gaagagccgt atgccggagc aattttggcg gcgatcgagg agtttggatt tgacgaaaag
    10681 tttcagacag tatatcttga ttcggaaaaa caaaatcttg acaccgaggg agagcaagaa
    10741 acagaagaga cggtaaaagg ggtgtatttg tggtttcata aaaatcttct tttgtacagt
    10801 aaagaaaata aagtcgatat agattttctt gaacagatga tttttatggc tgcgccggac
    10861 tgtgttgtgg gaaggaagga caatgtaaat atcgtaagct ggcttttgac ggattatcat
    10921 tttaagcagt cggacatgat ccccgaaatc gtggacgcag aaggaaaaac gactccgtgt
    10981 tttgcggcaa aagaagcgta tgcaggcgag tgggggtatc ttaagaaatg aaaaaacttt
    11041 tagatcggat ttttatagat ggtctgggcg gcatggcaca gggacttttt gctacgctga
    11101 tcataggaac gatcattcag cagatcgggt tgtttatggg aggcggcaca ggagatctta
    11161 tttatgaact tggaaagatt gccgcaggtt tgacaggtgc aggcatcgga gtcggtgtgg
    11221 cgcgaaaatt agaagcggga catctcgtaa tcgtttcggc cgcggttgtc ggtatgatcg
    11281 gtgcatttgc cgggaaaatc ttatccggac aggttcttgt ggatggaaat ctggttctgg
    11341 cagggccggg agagccgtta ggcgcttttg cggcggcgta tctggcgatc gagatcggga
    11401 tcttgatctc gggtaaaaca aaacttgata ttatactgac tccgctggtc tgcatcggaa
    11461 cgggatcggc agtcggtctg tttgtcggtc cgccgatttc tgaatttatg ttatggcttg
    11521 gctccttgat caactgggga acagagcagc agccgttttt gatgggaatt gtcgtttccg
    11581 ttttaatggg aatgatcctg acgcttccga tcagctcagc ggcgcttgga gtaatcctga
    11641 atctgtcggg actggcggca ggggcggcga cgatcggatg ctgctgtaat atgatcggat
    11701 ttgcggtggc tagttggaga gaaaataaat tcagcggatt tctggcgcag ggaatcggaa
    11761 cgtcgatgct tcagataccg aatatcgtaa gaagaccttt gatctggctt ccggttattt
    11821 tgtcaagcgc cgttttaggt ccgatcggaa caatgcttct tcatatgacg aacaacgcta
    11881 cgggatcggg tatgggaaca gccggacttg tcggtccgct tatgacatgg caggtaatga
    11941 cgcaaagtga agcgggagtg atcgtgctcg gaaagatcat tttgatccag tttatccttc
    12001 ctgcggcggt gacgcttctc gtttctgagt ttatgcggaa aaagaagtgg atccgtcagg
    12061 gagatatgaa attagagttg taagagaagt cttaaaaaac aatgaaatct atgacatgcc
    12121 ttaggattta tgttagaatg aaaaagtaaa ataactggcg gaggcagtct gaaatgacga
    12181 gtgaacaaca agtagaagct gcgctgaaaa gctatgagga tgtcgatagc tggaagacga
    12241 tcatttttct gtataacgca gcgctgaaag aagtaggaac aaagctggag attttgaatg
    12301 acgaatttca gcatgtacat caatataatc cgatcgagca tataaagaca aggataaaga
    12361 cgccggaaag cattgtgaag aaattaaggc ggtatggcca tgagacgtcg atcgagaaca
    12421 tggtacgcta tatcaatgac attgcggggg tacgtcttat ttgttccttt acatctgata
    12481 tttaccgttt ggcggagatg atcggcaatc aaagcgatct gaaagttctt tcgatcaaag
    12541 attatattaa aaatccaaaa gaaagcggtt ataaaagtta tcatatgctt gtatccgttc
    12601 cgatctttct ttccgacagc gtagtcgata caaaagtgga aatccagatc cgaacgatag
    12661 cgatggattt ttgggcgagt ttggaacata agatttatta taagtttgaa gggaatgcgc
    12721 ctgattatat aagcagagaa ttgaaagagt gtgcgaagat ggtgtcggaa cttgacgaca
    12781 agatgctctc tttgaatgag gcgattcagg cctgcatgat cagagacgat cagagaaaag
    12841 aaagtatgga agatgtgttt gacaatgtga tcggcgaaag aagaaaacag taaagaaaat
    12901 cttaagtaat cttaagtttt aagctttggg ggcaaaagat attgatgctt ttgcctcttt
    12961 tcgttatact gtatatttgg agggaaaaga cgaatgcagg aagaaaaaac agaaatagaa
    13021 cgctgcgatt ttatacatgt tcatgaagat gtagtaaaaa aagtaaagga acacattccg
    13081 gcagaaagag agctgaatga tcttgcggaa ttttttaaag tgttcggaga tgtgacgaga
    13141 ctgaaattgt taagcgtgtt gtttcattcg gagatgtgcg tgtgcgatct ggcaaagacg
    13201 ctgaatatga ctcagtcggc gatctctcat cagctccgca ttttgaaaca gatgaagctc
    13261 gtgaaaaaca gaagggaagg aaagacagta ttctattctc ttgcagacgg gcacattaaa
    13321 acgattatta atcagggaat ggaacatata caagagtaat aaaagaaaag ccggcgggca
    13381 gggaaagccg gcgggcaggg aaagccggca aacaggagaa agccgacgaa caaggggaag
    13441 ctgacgaaca gggaaaagtt gacggaaaag ggaaaaaggg atgacgagag aaaccagaaa
    13501 aagggaattg gagatcggta tcggtcttgt tttatatttg ctggcattct taataagcat
    13561 gcaggcaaag atgaaatcaa aggcggagat gcttttgttt ttactgccgt atattgtatc
    13621 ggcgatcagt atttataaag agcaggcaga gaaatttttg aaaaagcagt ttctggatga
    13681 aaacttactg atgatcatag cgactgtagg agcatttttt gtaggacgtt acaaagaagc
    13741 cgttgcggcg atgctctttt atcaggtcgg aaaacttgta gaagaaatct ctctgagcag
    13801 gacgaaaaaa tctattgcga aatttattga tattcgtccg gaatatgcga acctgaaaat
    13861 cggaaacaca gaaaagattg ttccgccgca ggagctgaat ttgagacaga cgatcgtact
    13921 gcatccggga gacaagatcc cggttgatgc ggttgtgaca agcggaacgg gaacagttga
    13981 tatgaaagcg ctgacaggag aaccggatcc tgtcactgtg cggatcggag acaggctgtt
    14041 cagcggaagc atcaatctga acagcgtgtt ggaagccaga gttgagaaac tgtacaatga
    14101 ttctactgcg gcgaagatta tgcggcttgt agaaagcgca aatgaaaaca aatcggagag
    14161 cgtacatttt gtagataaat tcacaaaata ttatacgccg atcgttattt tgatcgcgct
    14221 tttaactgtc attctcccgc ctatgatgtt tgtggaggcg aaggctgagt ggatatacag
    14281 aggattgatc attcttgtgg cggcatgtcc gtgcgggttg atggtttcta taccgcttgc
    14341 gtttttgggc gggataggat ctgcttcaaa gcagggcgtt ctgataaaaa gcggagctgt
    14401 tttggaagat ctgatccggg tagacacata tgtttttgat aaaacgggaa cactgacgga
    14461 aggcgtattt catgtgaaag atattgttcc gtataagttt gaaaaggaaa gactgttgga
    14521 gcttgcggct ttggcggaaa gttattcaaa gcatccggtg gcgctgtctc ttgcggaagc
    14581 ttatggaaag ccggctgaaa gtacgcggat caaagatgtg aaagaagaac cggggtacgg
    14641 tgttcatgcg tttgtggacg gactggaagt ttatgccggg aacacaaggc tgatgaaccg
    14701 ggaaggaatc ctctacgcac agacggaaaa agacggaact gccgttcatg tggctgttga
    14761 cggaaagtat gcgggataca tattgattgc ggatacgatt cgtaaagata cgggaaaact
    14821 gatgcgctgg atgcgaaaga aaaatatggg aacggttatg ctgaccggag ataacgagcg
    14881 tacggcggaa aaagtggcgg cagagctgaa aatcgacagc gtttatgcgg agctgatgcc
    14941 ggaagataaa gtgcagcttc tggaagagtt ttgcaatgac cgcatggaaa atgaaaaatt
    15001 ggcgtttgta ggagatggaa tgaatgacgc gcctgttttg gcgcttgcag acatcggcat
    15061 cgcgatgggc ggtctcggcg cggatgccgc gcttgaggcg gcggacatta tccttatgga
    15121 agatgagccg tcgaagatta taaatgcggt gcggatcgca aaaggaacga tccggtctgt
    15181 acgggaaaat gtattgtttg cgatcggtat gaaagtattt ttagtcatac ttgcgttttt
    15241 cggtcttgtg acgatgcaaa gcgctattat agcagacatg gttgtgatgg taattaatat
    15301 tttgaattca ttttggatga tgaagtatcc ggaatcgggg gtgtaaacga atcgtgaaaa
    15361 tcaaaaaaca gatcgtgttg gcggcagttc tttgtatggc tgtgccgact gtggcatcag
    15421 gctgtgcaaa cagtgcgaaa tcaggtgtag aggcattgga ggcaggagat tataaagagg
    15481 cgcaggcaca gaaaaactga ctgaagaaaa ggataagaag aaatcggcag aggggtatcg
    15541 gggacttgcg atgacatatt atgagcagga ggagtatagc tcggctctcg atgcgtttaa
    15601 aaaagctgtt gacacaggag tagtacagac gactcagatc tataatctga tgggtatttg
    15661 tgcaatgaag acagaggatt acgaggcggc tcttgaatat atacaggcgg gtcttgccat
    15721 ggcggaaaca gatatgtcag gtgaagaaga gaagaattcg gaaaatggaa aagattctgc
    15781 tgaaatgatt caggagatga gatataacga agttgtctgt tatgaaaaac tggctgattg
    15841 ggaaaacgcg aagcaaaaag cgtccgagta tctgatcgaa tatccggaag atacggcgat
    15901 ggaaagagaa gccgagtttt tggaaacgag ataaacaccg tttggcaaaa gaaagttctc
    15961 atgaaaggga atttttcgtg aaaggaaaat ctacgtaaag ggaaaatcta tgtggaggga
    16021 aaatctatgt ggagggaaaa tctatgtgga gggaaaatct atgtggaggg aaaatctatg
    16081 tggagggaaa atctatgtgg agggaaaatc tatgtgnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnngagg gaaaatcttt gtggagggaa aatctttgtg aagggaaaat
    16261 ctttgtgaag ggaaaattct cataaaagaa aaatcctcat aaaagaaaat tcggagaaat
    16321 aatatgtatg agatggagca aatcgacgag agggtgatcc tcgtcggaat cgatacggga
    16381 aatgaagatg cggcaaaccg ttcgcttgac gaactttcgg aattggcgaa aactgcaaaa
    16441 gcggctgtag tcggcaggct gatccagcca agagagagcg cgcatccggg aacatatatc
    16501 gggaaaggca aattgacgga actcaaagat cttatatggg agactaacgc gacggggatt
    16561 atttgcgacg atgagctgac gagcgcacaa ttaggcaacc ttgaagaaga actgtcatgt
    16621 aaaattatag acaggacgct tttgatcctt gatatttttg cggcgcgggc agtatcggga
    16681 gaaggaaaga tccaggtgga gcttgctcag cttcgataca gagcgtcaag actggcgggg
    16741 cttggcagat ctttatcccg tctcggcggg gggatcggaa cgagaggtcc gggagaaaaa
    16801 aagctggaga tggacaggcg tttgatcaga gaacggatca gcagactgaa aaaagaacta
    16861 aaagacgtgg aaaagcatcg ggaattaata cggacacaga gaaaacagtc gggattaaag
    16921 gtggcggctt tagtaggata tacatcggcg ggaaagagca gtatagaaaa tgcgctgaca
    16981 aatgccggca ttttagaaga cgccatgctg ttttctacat tagatactac aacccgctct
    17041 cttgttctcg ataatacgca ggaaatatta gttacagata cggtcggttt tataagaaaa
    17101 ctgcctcatc accttgtgga agcgtttaaa agtacgttag aagaagcgaa gtatgcggac
    17161 attattattc atgtggtaga tgcgtctaat ccgcaaatgg atgagcaaat gcatgtcgtt
    17221 tacgatacat tgcgtcagct tggagcggca gaccgaccgg tgatcacact ttttaataag
    17281 caggataaac tcgagtcggc aggcagctat cgggattttc aggcggaata ttcaattccg
    17341 gcttcggcaa aaacaggaga aggtcttgca gaactaaaaa aggcgcttct cgagatcgta
    17401 cgaagagaac agatctatgt ggagcgtttg tatgatttct cggaagcatc gaaaattcag
    17461 cttatccgaa gccgcggaca acttttgtcg gagaagtatg ttccggaagg gattgaagtg
    17521 aaagcgtacg ttccgaaaga tatttatggg aaggtttgta tataaaaaga gcagttgtga
    17581 aaatagaata ataaaaagtt tttctgtcag ccgcggagcc gtgtgctggc agttatttgt
    17641 aatggtggca atacaaagtc tgggtttgta tataatgccc aatatggata gcaagaaaaa
    17701 aggaaaagat gaattcaaat tggttaatag taataaaaaa atgtgaaaat tgatgtaaaa
    17761 aaggaaaaga gttgagtttt ttatgtcaaa attatataat gttacgaaga gtgtaattca
    17821 gctcattaaa attcaagaaa gcagagaggt aaaaaatggg aagaaaaatg aaaaagttaa
    17881 cgcgcgccgg cgcgggagct cttgccggag taatgatctt cggattggca gctccgcttg
    17941 cacctctaag cgttgaggcg gcaggagaaa ttgaaaatct tgcacttaac aaaacagcaa
    18001 ccgcaagtga gcaggaaaca gatagttatt ctgcggcaaa ggcagttgac ggtgtggtaa
    18061 acagagatgc ggcaaaaccg cagtcacgtt gggcgacaaa tactcattcg aatcaggaag
    18121 cgcaatggct gaaagtcgat ctgggagaaa aaaagacatt tcggtcattt gttcttgcat
    18181 gggaaagaag aaatattaca agttataaaa ttcagatctc cgatacggga gaagataatg
    18241 atgattcaaa atggacgacg gtttatgaga aaaaagccgg ggataaaatc tccggaatta
    18301 atgaaaatat ccgtttggat gagccaaaag aagcaaaata cgttcgcttg tacatagatg
    18361 gatatacagc agacgcagtg ggcgacagcg tgagctggca gtctgtttcc ctttatgatt
    18421 tccagattta cgcagatgaa atccctgatg acgttcttcc gaatgaaaac tattgcttgg
    18481 aaggaacggc agaagcgaat gattatgaaa aaacagatgg ggatacgcag gcaccatcta
    18541 aggcaatcga tggaaatctg acaacacgat gggcaactaa tcctgataac gaaggtaaag
    18601 gagcagctga tcgtacattg actgtgacac ttccggttac gcagagagtg cagcacttca
    18661 agattaactg ggaaaaaaca aatattaaag agtatacgat tgaagtaaaa gacagctcaa
    18721 ccggagagtt taaacaggtt tatgagaaca caaagcagat tacatcttta gaggaaatta
    18781 tttctttaga tgcgccagta tgggcaaaag aaattcgttt gaatgtgaag agctatggcg
    18841 aagctgtgcc gaattggtat aacgtttctg tagcagagtt cggcgcatat gcagtgaaac
    18901 cggaagcaac tccggatccg gaaacgccgg aagatgagac gttgcaggct cttgcaaact
    18961 ccatcgcagc tccgcagttt aatgccgata agacgcagtt gacatggaaa acaccggagg
    19021 gagtaaaagt agaattcctt gcggattatg aacagatcgt cggaagagac ggtaagatct
    19081 atcagccgct cacagataca gagatcaaag gtgtctataa ggtgacaaag acggcagcgt
    19141 cagcaacgga taaaaaagag aaaaattatg cagaagcgac agtagagcat acgatcacaa
    19201 ttccggggaa acataaagaa gcaggtgtaa atgcaaaacc gacagttgta cctgaactgg
    19261 ctgagtggta cggcggagaa acagaaggaa atgtgacaat cagcggcaag atcgttgttg
    19321 atccgaatgc aggtgatctg aaagctgcgg cagatgcttt ggcgacagat tatgaagcag
    19381 agcttggaaa gaagatgact gttgcaacag gcagcaatcc ggcagcagga gatatctact
    19441 ttacacttga taagagcgat aacggacttg gagaagaagg ctatgttatg gagtctgatt
    19501 catatgtaaa ggttactgcg gctcaccaga caggcgctta ttgggcaaca cgttccatta
    19561 ttcagattgc ggaactgaac gaaggaaaac ttccgaaggg aacaacaaga gattatccga
    19621 agtatgaagt tcgtggattt atgcttgatg ttgcacgtaa gccggtttct atggaaactt
    19681 tggaagatgt agcaaaggaa atggcttatt ataaaatgaa tgctcttcat gttcatttaa
    19741 acgataacct tattttctat gaagattttg caacgaccga agaagcgaga gaaaaagcat
    19801 atacaggttt ccgtttggag tccgatatta agaaggacgg caatggagga aagaataaag
    19861 cagatctgac aaataaagat atgcnnnnnn nnnnggagca tttacaaaag ttcgtcctga
    19921 tctgatgctt cagaaaacaa tggaaggaaa tgcaaaacgt gctggagagc agtttgacct
    19981 ttcagatgag aaatatgcgg attcgcttgc atttgttcag agtgtgtggg acgaatacct
    20041 gaacgatgat atgtttgatg acagtatgac attacatatc ggaacagatg aatactacgg
    20101 agaagctaac tgctttagaa aattcacaga tgatatgatc aagcatctca aaaagaaagg
    20161 ctacaaggtt cgcatgtggg gaagtcttac aaataagaaa gaaaaccagc cggatgcata
    20221 cccggttgac ggagagggtg tacagcttaa catttggtca gaaggatatg caaatccaca
    20281 ggatatgtat agcaaagggt atgaattgat taatacgttg gaaggaaatt atatcgttcc
    20341 tgcggcaggt tattataatg attatgtaaa tagtcagtct gtatatcaga attggcagcc
    20401 gaataaatac agtaatgtga cattgccggc aggatctgat caggtactag gcggtacatt
    20461 cgcaatttgg aacgacagca tcgataccag agcaaacgga atttcggaag ttgatatcta
    20521 tgatcgtttc gttaaggcgc ttccggcttt tgcaagcaaa aattggggag acggaacgga
    20581 taagaactgg agtgaagtga aggcagtttc ggatcagttg ggcgatgctg caaacagcaa
    20641 cccgtaccat aaagcaactg ctgatgaatc cggcaaatat atggaatatc agtttgaaaa
    20701 cgagaaagac agttcagaca ataagagaga tcttgaactg gcaggcggcg caaaacttga
    20761 aaaaggaagc ctgaagctga caggtggaga aagctatgcg acaactccga tcgataagct
    20821 tgctacaggc aatacgctga aattcgatat cacattagat aaagaagcta aagcaggaca
    20881 gattctgttt gaggcagata atttaggtga taacggcgat tatgttcatg atatccgtat
    20941 tatggaagac ggaagacttg gattcagaag agagctttat gactattact tcgattataa
    21001 acttccggta ggcgagaaag tccatcttga aatcagaaca gatggcacaa agacaagctt
    21061 gatcgttgac ggacaggaat tacaggctac aggagtttac cgaaac
//
LOCUS       DBMDXF010000007        20001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_845_length_20001_cov_1.955226, whole genome shotgun sequence.
ACCESSION   DBMDXF010000007 DBMDXF010000000
VERSION     DBMDXF010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 20001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20001
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_20001_cov_1.955226"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(110..1165)
                     /locus_tag="ACZPD4_00630"
     CDS             complement(110..1165)
                     /locus_tag="ACZPD4_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1377617.1"
                     /translation="MRINEIIKERRLAKGFTQEQIANYLGVTAPAVNKWEKGTSCPDI
                     VLLPALARLLDTDLNTLLSFQDDLSEKEVALFLNEVSEAAKKDGFEAGYSLAIGKIKE
                     YPTCDLLIGNVAMLLNGLLLFQGNRIDSYEKYEEEIEALFQRVMQSDRIDIREQAQAY
                     LISKLMEKQDYEQAQKVLDTISKKRVLDREQLQANLYIAQGELEKAAKLTEEKLLSAT
                     TEIHGALMTLMEIALKEKRMEDAEYIADIDKQAAQVFDLWEYNSYGAQFQLYVATKKR
                     AKAVKILLPMLRSLTKKWDINSSPLYRHIQTKEVDKSFGSQLQKALVQSIETDEEMKF
                     LKDSEELQSFIKEAELK"
     gene            complement(1365..1904)
                     /gene="nrdG"
                     /locus_tag="ACZPD4_00635"
     CDS             complement(1365..1904)
                     /gene="nrdG"
                     /locus_tag="ACZPD4_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1377618.1"
                     /translation="MYYGEIKKCDIANGEGVRVSLFVSGCTHHCPGCFNADTWNFKYG
                     SEYTDQTEKEILEALSPSYINGLTLLGGEPFEPENQKTLVGLLRKVRESYPEKNIWCY
                     TGYLYAEQLLNDSRARCEYTDEMLSMLDVLVDGEFVEALKDIRLVFRGSSNQRVIDVK
                     KSLGQNEIVMWQPKVERGV"
     gene            complement(2088..4247)
                     /gene="nrdD"
                     /locus_tag="ACZPD4_00640"
     CDS             complement(2088..4247)
                     /gene="nrdD"
                     /locus_tag="ACZPD4_00640"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1377619.1"
                     /translation="MKIIKRNGAEEVFDIKKIVIAVTKADSSSEGRSLTDSQIEDIAE
                     FVEFKCNKLNRAVSVEEIQDLVEDQIMATGAFELAKRYVRYRYKRSLVRKANTTDNRI
                     LSLIECNNEDVKQENSNKNPAVNSVQRDYMAGEVSRDLTQRMLLPEDIVEADKEGIIH
                     FHDSDYYAQHMHNCDLVNLEDMLQNGTVISGTMIEKPHSFSTACNIATQIIAQVASNQ
                     YGGQSISLAHLAPFVQVSRDKIRAEVLQEMEMVGIDYPKQVLNDIVEGRLKKEIIKGV
                     QTIEYQVITLMTTNGQAPFLTVFMYLNEAKDEREKKDLAMIIEETLKQRYKGVKNEAG
                     VWVTPAFPKLIYVLEEDNIDEGSPYFYLTELAAKCTSKRLVPDYISEKKMKELKEGNC
                     FPVMGCRSALSPWKDENGNYKFYGRFNQGVVTINLVDVALSSGGDEDKFWKVFDERLD
                     LCYRALMCRHNRLKGTLSDAAPILWQFGALARLKKGEKIDNLLYGGYSTISLGYAGLY
                     ECVKYMTGKSHTDPSATQFALDVMKYMNKACDRWKEETNIGFSIYGSPIESTTYKFAK
                     CLQKRFGIIPGVTDKSYITNSYHVNVCEPIDAFDKLKFESQFQALSTGGAISYVEVPN
                     MQDNIPAVLQVIRFIYDNIMYAELNTKSDYCQECGFDGEIQIVTSEDGKLEWECPHCG
                     NRNQDTLNVARRTCGYIGTQFWNQGRTQEIKERVLHL"
     gene            complement(4750..6168)
                     /locus_tag="ACZPD4_00645"
     CDS             complement(4750..6168)
                     /locus_tag="ACZPD4_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1377620.1"
                     /translation="MLEQIDQIIKVIDGAVWGLPLIILILFTGFLLTTRLGLLQIRHL
                     GKALKFMFKNEEDGQGEVTSFGALCTALSATIGTGNITGVATALAAGGPGALFWMVIA
                     AFFGMATKYAEGLLAIKYRTIDKDGHVLGGPFYYIENGMGKNWRWLAKIFAFFGAGVG
                     LFGIGTFTQVNSISSAVKNFFDPESSNVVFSAFGHDYTMATVVTGLILTLCVGLVVIG
                     GIKRIAKVSEIVVPFMAVLYVALGAIIIITNITAVPAALVSIIKSAFTGSALAGGAMG
                     TMVVAMQKGIARGIFSNESGLGSAPIAAAAAKTKEPVRQGLVSMTGTFIDTIVICTMT
                     GLSIVIAGTWMNPELEGVEITVAAFQKGLPFPPIVASFSLMLCLVFFAFTTILGWNYY
                     GERCIEYLFNRNKSVVKGYRWLYILAVFIGPYMTVAAVWNIADIFNALMAFPNLIAIL
                     ALSGVVVRETKDYFARQKKQSK"
     regulatory      complement(6171..6302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(6452..11839)
                     /locus_tag="ACZPD4_00650"
     CDS             complement(6452..11839)
                     /locus_tag="ACZPD4_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-alpha-N-acetylgalactosaminidase family
                     protein"
                     /protein_id="HIR1377621.1"
                     /translation="MKRKVVLRLLSLVMVASMTGTMLPSNIQTASAEEVDVTEKTAAL
                     EDGINDAWTQDNKADGDTVTVENGWLHIKSAAGNRNNPGTRPQMVVNPNTFDFNKPGY
                     FSFTLKSNNANTGISDSDRVGVYLGYNTDQNGMYIGYDNGGWFWQKYKGGNGDYYQQT
                     RKPAPTKDQEVKVRIDWTADHKMTFTLNGEVVFDKEDFSGIADSLGNKIAIKAGSWGQ
                     IGSDVLLKDIHYTGQEEAVTYTVTGSVTDESGKALEGAVVTTGNLTAETDKDGKYSLQ
                     LGAGKHELTITKAGYQTATTSVTVTEGNVEAKAVKLEKTAEIETEKLSTADMDVYVAK
                     NFPSVVKYEMKKGDLNGKTFYGQTSAINTVRINGTDVKLSKGDVKATIKGDKATYEMT
                     VKNEEKHIDAVLTAELTAKDNTVSFEITKVENKLTEGKPGTALESGKVGNPIQTIEIP
                     NHSLVSVNSTQKNANLIGAAMSTQTKVSGDEYVEVNANTPARERDYMYAFVSNDEMSA
                     GLWSNSEFEGRNAGASSSGGSNNTRVMSVSEKKDGYVSMGLGSSAWYWHRVMTDSHNR
                     TWVLEETENPKMKVVITGNCNGDKNVDWQDGAVAFRDIMNNPFKSEEVPELVAYRIAM
                     NFGSHAQNPFLTTLDNVKRVAMHTDGLGQSVLLKGYANEGHDSAHPDYADIGKRIGGP
                     EDMKTLLEKGADYGAKFGIHVNAGEMYPEAKAFKDDNVRRNKDGSLRYGWNWIDQGIG
                     LDSIYDLATGEREARFDELHEILGGDGKDMLDFIYVDIWGNNTGSDNDDSQQTRKLSK
                     EINDNGWRMSNEWGGANEYDSTFQHWATDLTYGGEGAKGENSDVMRFLRNHQKDSWVG
                     DYPTYGGAAVAPLLGGYNMKDFEGWQGRNDYDAYITNLYTHDLTTKFIQHYQIVDWED
                     GTPVNVGGAVNWTPEMKITLKDEDGSTLVLERGSNDPAQAAYRDRTMTLDGKVIARGA
                     VSQGDRTDDDIRNGNKKGTESYLLPWIWDAKTGEKVKSEDEKLYHWNTQGGTTQWELP
                     DSWADLKDVKVYKLTDLGKTEEKTVNVVDGKITLEAESEVPYVVCKGKKENIKVTWSE
                     GMHIVDAGFNSGSLDMWTKAGEGTAQIAKSQHSNPMMKLDGKVSMTQKLTDLEAGKQY
                     AVLVGVDNRSDAKASVEIKSGDKVLGSNYTTRSIAKNYVKAYTHNTNSSTVDGSSYFQ
                     NMYIFFTAPKSGDVTLTIAREAGEGSSYFDDIRIVQNDSKNITTNEKGEVVKFEQNFE
                     KSVQGLYPFVVGGIEGVEDNRIHLSERHDKYTQAGWDVKKMDDVLDGDWSVKINGLTQ
                     RSTLAYQTIPQNFRFEPGVTYNVSFDYQAGSDGIYAAAVGVGEYNGNVQLKELPMSMG
                     KEKDGHFTMQVTGDSTGQTWFGIYSTEKAPDLQGVSPDAAEANFGGYKELVLDNLVIE
                     KVTEEVTKEKLAALVAEAEEKYKEIDYRPEIWSSFQDVLKEAKAVLDKEGASQDEIEK
                     AYYELKAAMVTMDNSAGIDATDDSKDLPKEQMTATAGSEQAQEGGEGPASNVLDGNAD
                     TIWHTVWAGTPIENHWLNLQLDKPATVSGLRLQQRSGRNGIIREAEIWVKKAGAADYE
                     KVADTSFGGTGWQAVAFEEVKDVTDVKLVPTATTGDEANKFSAAAEIRVMGRFQDEEV
                     EVNKADLTALVEKAEALKEKDYTSETWKPFAEALKEAQAVLAKEDASQAEVDAAQTSL
                     QTAMDGLKKPGTTDNKNPGTTDNKNPGAPNKGQAVQTGDATSFFGWGAAGIFGLFAAA
                     AAFFERKRRRQ"
     gene            complement(12015..12857)
                     /gene="folK"
                     /locus_tag="ACZPD4_00655"
     CDS             complement(12015..12857)
                     /gene="folK"
                     /locus_tag="ACZPD4_00655"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR1377622.1"
                     /translation="MKKDYDEIRIEDLEVFANHGVFPEENVLGQKFLVSAVLYTDTRR
                     AGLTDDLSASIHYGEVSAFIDRYLREHTFQLLERAAEALAEELLLHTAGLEKIRLEIK
                     KPWAPVRLPLKTVSVVIERQWHTAYIALGSNMGDREKYITDAVEALGALKGCVVEKCS
                     SFIETPPYGVTDQDDFLNGCLKLRTLFYPKELLRELNRIEMEAGRERIVHWGPRTLDL
                     DIILYDDLVYEGDELCIPHVEMHKRDFVLRPLFEIAPYVRHPAYGKTVAEMLEEVREN
                     AQKE"
     gene            complement(12978..13793)
                     /gene="folP"
                     /locus_tag="ACZPD4_00660"
     CDS             complement(12978..13793)
                     /gene="folP"
                     /locus_tag="ACZPD4_00660"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR1377623.1"
                     /translation="MIIGRREFDTEHNTYIMGILNVTPDSFSDGGRFDKMDAALLHAE
                     KMIKEGADILDVGGESTRPGHVQITDEEEIARVVPVIEKLKKEFDVPISIDTYKSAVA
                     EVAVCAGADLVNDIWGLKYDEKMADVIAKHDVACCLMHNRNEAVYDSFLFDFMKDMKE
                     CVKIADKAGIARDKIILDPGVGFGKTYEMNLEIIQKLEIMHEFKLPILLGTSRKSVIG
                     LTLDLPADEREEGTLVTTVYAVQKRCAFVRVHDVEKNKRAILMTQALMREHEV"
     gene            complement(13790..14281)
                     /locus_tag="ACZPD4_00665"
     CDS             complement(13790..14281)
                     /locus_tag="ACZPD4_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1377624.1"
                     /translation="MDRVNAVICHPLYLEYYHRLERAEKERIFCRHQMSHLLDVARIA
                     YIRNLEKGYGFRKDVIYAAAVLHDIGKALQYEEKVPHEIAGERIAGEILDTLPKETGF
                     SEEEKRTIKNAVRGHRRLRDGEDTLEKLLYEADKASRLCLSCPANRECNWSEEKKNME
                     IKI"
     gene            complement(14318..14878)
                     /gene="folE"
                     /locus_tag="ACZPD4_00670"
     CDS             complement(14318..14878)
                     /gene="folE"
                     /locus_tag="ACZPD4_00670"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR1377625.1"
                     /translation="MIDQEKVKKAVRLLLEGIGEDADREGLIETPDRIARMCEEIYGG
                     YEEDAKEHLCKTFHVENSDMVVEKDITFYSMCEHHLLPFYGKAHIAYIPDGKVAGLSK
                     LARTVEVFARRLQLQEQLTGQIADALMNELKAKGAAVVIEAEHMCMTMRGIKKPGSRT
                     VTFAKRGLFETDPALEERFFRMLERG"
     gene            complement(14903..16240)
                     /locus_tag="ACZPD4_00675"
     CDS             complement(14903..16240)
                     /locus_tag="ACZPD4_00675"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1377626.1"
                     /translation="MKLIQERNKSVTYKEARVYLDELSKYGSVLGLDAIRGLLRELGN
                     PQDDLRFIHIAGTNGKGSVLAYTSTILSEAGYRIGRYVSPTVVSYRERIQVDGEMISK
                     HAFAEETEKVQDAIVRLKEAGKQLPTVFEAETAIAFLYYKRMKCDFVVLESGLGGMED
                     ATNVVKNTVCAVFSPISMDHIGVIGNTLSEIAENKAGIIKPGCIVVSAPQKQEVREIL
                     EQKARQFGCPIIYAEPEKAVVLEEDYRGMRFSYKDSENISIRLSGKYQLINAVTAWEA
                     VHVPGLLKKEEKRAVETGFFDTEWFGRFTCISERPVFLVDGAHNEDAAKRLRESIEAY
                     FPGRKLIYIMGVFQDKEYEKIIRLTIPYAKCVYTVDLPNKERTLSAQKLKEAVEAQSE
                     YVPAYAVGEIKQAVKEALENADEDDVILAFGSLSYLGKVKEAVEKEQDKRQMY"
     gene            16416..16808
                     /locus_tag="ACZPD4_00680"
     CDS             16416..16808
                     /locus_tag="ACZPD4_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1377627.1"
                     /translation="MITIDYQSKLPLYEQIAKRFQTLILKGALPPDSQMPSVRSLAMD
                     LSINPNTIQKAYSLLEQERYIYPVKGRGNFVSDTTLLVEQTKKTLLENVSRLIANGKE
                     LGVKRAEFTQLVDRIYRKEDSHDRVKNA"
     gene            16786..17688
                     /locus_tag="ACZPD4_00685"
     CDS             16786..17688
                     /locus_tag="ACZPD4_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377628.1"
                     /translation="MIELKTLDKTFQMIHASDHITATIPDGMVFGLIGSNGAGKSTLL
                     RMISGILKPDEGEVLIDGESVFENPSVKSQICFLSDTPYFFPNADIQQMRNYYMLCYP
                     SFNRKLFDSLTDRFNLDPKRKISSFSKGMKKQVSILLGLCSGTKYLLCDETFDGLDPV
                     VRQAVKSLFAAEILNRDFTPIISSHNLRELEDFCDSIGLLHQGQLLLTQSLDQIKCSV
                     CKLQCVISDPHKEQQFLSSIKILKMERSGSLLTLTLRGSHSDILSAVQTYDPVFAEVL
                     PLTLEEIFISETEAAGYDIKDFLY"
     gene            17663..19885
                     /locus_tag="ACZPD4_00690"
     CDS             17663..19885
                     /locus_tag="ACZPD4_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6449 domain-containing protein"
                     /protein_id="HIR1377629.1"
                     /translation="MISKISYIKLIREDIRHRGWLAALSGVLLFLSMPVYTLIYLDSY
                     SEDPEVLKEFADTFSGFINGYNSAFLIILIGILAFAVGISGFQFMHKKEKLDFYHGLA
                     LKRHQLFAISYLAGLFLFLIPYIVCTVLTILVGAVKGIMTLSLAGECFLTMGTGILAF
                     LLIYHTCIFAGMLTGRSVTMFLGTLVIGVYPSLVLTLLPSLRDAFFQTTYNVHDSIPE
                     KLSEYLSPFPLFIRMLEQNNSYSNVFMITAALVLIVLLLAASIMLYRIYPSESAGNAL
                     AFKFTPPVFKVLISIPTAVFISLLVKSFIGASGTKWIIILSMLAIVLLCGLIEFIYWQ
                     DLKMLLKGWRSSVIAIGFVAVLLAVFQFDFTGYDTYLPKEEKIARIGTLPDSFASYFW
                     YPEDYTSEDDRESFVLSDDVDVLYNLAQSGIENVKKGITPNTLYSSQTSMQTDKDSDN
                     YICAAFCFELESGKKVYRNYCVFYNETLEAFTKLCQKEDYRKELFPIFHVEKENVSSV
                     SLQDIYITPEELLLTAEQRTELLSAYEKDVLNTDIRKLGSEVPIGEFSLTIRHVPNKE
                     TNTNQPFHSWVNGTREWNAAVNTAFPVTSSEDETNYKTFSIGSLYIYEDYENTLSCLE
                     KYGYKIKRLIRAEDIDSVTLYLSGDSMKNKKWNDLLASFSSAKDENNSDPDKAAYEQS
                     DEKDATSMFTITAPEDFRLLAEHLYLNPSDFLDRGGTSRDYAEVTFKKENNTFTYLLK
                     "
BASE COUNT         4931 a         5205 c         3491 g         6374 t
ORIGIN      
        1 taataaagat atctatgaca tctatacaaa gaaagcagtt tcccgaattc aggtttttat
       61 attttcaaaa cctctatcgg aaaactgctt ttttcttttc ctcagttatc tacttcaact
      121 ctgcctcttt tataaagctt tgtaattctt cgctgtcttt taaaaatttc atctcttcat
      181 ctgtttcgat cgattgaacg agcgcttttt gtaactgcga accaaacgac ttatcaactt
      241 ctttcgtctg aatatgacga tacaaaggcg aactgttaat atcccatttt ttcgtaagtg
      301 atctcagcat cggcaaaagt atctttaccg ccttcgctct ttttttcgta gccacataaa
      361 gctgaaactg tgcaccgtaa gaattatatt cccacagatc aaatacctgc gccgcctgct
      421 tgtcgatatc cgcaatatat tccgcgtctt ccatccgctt ttctttcaat gcgatctcca
      481 ttaacgtcat cagcgcccca tgaatttcgg ttgtggcaga caaaagcttt tcttctgtca
      541 gctttgccgc tttttctaac tctccctgcg caatgtacaa atttgcctga agctgttctc
      601 tgtcgagcac cctctttttt gaaatcgtat ccaacacttt ctgggcctgt tcataatcct
      661 gtttttccat caatttagag atcagatacg cctgcgcctg ttctctgata tcaatccggt
      721 cactttgcat tacccgttga aataaagctt cgatttcctc ttcgtatttt tcatatgaat
      781 caattctgtt tccttgaaac aacagcaggc cgtttagcag catcgccacg ttaccgatca
      841 gcagatcaca ggtagggtat tctttaattt tcccgatcgc aagagaatat cccgcttcaa
      901 agccgtcttt ttttgccgct tcagacactt cattcaaaaa cagcgcaact tctttctccg
      961 acagatcatc ctgaaaagac agcaaggtgt tcagatccgt atcaagaagc ctggcaagcg
     1021 ccggaagcaa gactatgtcg ggacacgacg ttcctttctc ccatttattg acagccggag
     1081 ccgtcacccc aagataattc gcaatctgct cttgtgtaaa tccttttgcc agacggcgtt
     1141 cttttataat ttcattgatt cgcataataa taggcacctc tcttgtctaa gctcaaatac
     1201 atgtctaaat caaatataac atctcttctt cacttataca attaaggcgc cgttaattta
     1261 tccttatgtt tttttaactg ccggtatacc gaaataactt tcccagcctt tatattttgt
     1321 atgatatccg tgattctctt ttggtattaa atcattcttt tcatctacac gccccgctct
     1381 acctttggct gccacatgac aatctcattc tgaccaagac tttttttcac atcgatgact
     1441 ctttgattcg atgaacctct gaacacaaga cggatatctt ttaacgcctc tacaaactct
     1501 ccgtccacaa gcacatctag catagagagc atttcatctg tgtattcaca tcttgcgcgg
     1561 ctgtcattta aaagctgctc agcatacaaa taccctgtat aacaccatat atttttttca
     1621 ggatagctct ctcttacttt tcggagaagt ccgacaagcg tcttttgatt ctccggctca
     1681 aacggttcgc cgccaagtaa tgtcaatcca ttaatataac ttggcgagag cgcctccaaa
     1741 atctcttttt ccgtctgatc tgtatattcg cttccgtatt taaaattcca agtatctgca
     1801 ttgaaacatc ccggacaatg atgcgtacac cctgaaacaa acaaagatac tcttacgccc
     1861 tcaccgtttg caatatcaca cttttttatt tcgccgtaat acacctgaat tcacaccttt
     1921 cctctatact ctcttatccg tttgatctat atatgcaatg aatcaccgga agtgccaaaa
     1981 ggtacattcc gggattgtaa tcggtcgccg ttaaaaaaac aggataccaa gcagggcatc
     2041 ctgtttcttt tgattcattt atccctcttt gtttgtcacc cgatatttta caggtgaagc
     2101 actctttctt tgatctcctg tgttcttccc tgattccaga actgtgttcc gatgtaaccg
     2161 catgtacgtc ttgctacatt aagagtatcc tgattgcggt ttccgcagtg cggacattcc
     2221 cactcaagct ttccatcttc agacgtcacg atctggatct ctccgtcgaa tccacattcc
     2281 tgacaataat cactcttcgt attcagttcc gcatacatga tattatcata aataaaacgg
     2341 attacctgaa gaaccgccgg aatgttgtcc tgcatattcg gcacttctac ataactgatc
     2401 gcaccgcctg tagaaagcgc ctgaaactgg gattcaaatt tcagtttgtc aaacgcatcg
     2461 atcggctcac atacattaac gtgataactg ttcgtgatat aactcttatc cgtcacaccc
     2521 ggaataatac cgaatcgttt ctgaaggcat tttgcaaatt tgtatgtcgt actctcaatc
     2581 ggagaaccgt aaatactgaa acctatattt gtctcctctt tccatctgtc gcacgcttta
     2641 ttcatgtatt tcattacgtc aagcgcaaat tgtgtagccg acggatctgt gtgcgatttt
     2701 cccgtcatat atttcacaca ttcgtaaagt ccggcatatc caagagagat cgtagaatat
     2761 ccgccataaa gcagattatc aatcttctcc ccttttttca gacgtgccag cgcgccaaac
     2821 tgccaaagga tcggagccgc atccgaaaga gttcctttca gacggttatg tctgcacata
     2881 agagcccggt aacaaagatc cagtctctcg tcaaacactt tccagaattt gtcctcatct
     2941 ccgccggagg acaatgctac gtccacaagg ttgatcgtca caactccctg attaaagcgt
     3001 ccgtaaaact tataatttcc gttttcgtct ttccacgggc taagagcgct tcggcatccc
     3061 ataaccggga agcagtttcc ttcttttagt tctttcatct ttttctcgga aatataatcc
     3121 ggaacaagac gttttgacgt acattttgcc gcaagctccg tcagatagaa atacggagaa
     3181 ccttcgtcga tattatcttc ttcaagcaca tagatcagct tcgggaacgc cggcgtcacc
     3241 catacgcccg cttcgttttt cacgccttta taacgctgct ttaacgtctc ttcgatgatc
     3301 atcgcaagat cttttttctc ccgctcatct ttcgcttcgt tcaaatacat aaatactgta
     3361 aggaacggag cctgaccatt cgtcgtcata agcgtgatca cctgatattc gatcgtctga
     3421 acgcctttta tgatctcttt ttttaagcgg ccttccacaa tatcattgag cacctgcttc
     3481 ggataatcga tccctaccat ttccatttcc tgaaggactt ccgcacggat cttatctctt
     3541 gatacctgaa caaacggcgc gagatgcgca agcgaaatac tctgtccgcc atactggttg
     3601 ctcgccacct gtgcgatgat ctgtgtcgca atattacatg ccgttgaaaa gctgtgcggt
     3661 ttctctatca ttgttccgct gatgaccgtt ccgttctgga gcatatcttc cagattgaca
     3721 agatcacagt tatgcatatg ctgcgcataa taatccgaat catgaaaatg gatgataccc
     3781 tctttatccg cttcgacaat atcttccggc aaaagcattc tctgcgtcag gtcacggctg
     3841 acctctcctg ccatataatc tcgctgcacg ctgttgaccg ccggattttt attggaattt
     3901 tcctgtttga catcttcgtt attgcactca ataagtgaaa ggatcctgtt gtccgtcgta
     3961 tttgccttac gcacaagcga tctcttataa cgatatctca cataccgttt tgccagttca
     4021 aatgctcctg tcgccatgat ctggtcttcc acaagatctt gtatctcttc aaccgaaacc
     4081 gcacggttca gtttattgca cttaaactcg acaaactccg caatatcttc tatctgcgaa
     4141 tcggtcagac ttctcccttc cgatgatgaa tccgcttttg tcactgcaat tacgatcttc
     4201 ttaatgtcaa atacttcttc ggctccgttt ctcttgataa tcttcatatt tcgcctctcc
     4261 tcatctatct ttttttcaga tgcttttccg ctttctccgc ctttttgcgt atttcacgga
     4321 acattttcct catgtcttgc atcgcacggc tgtcatatcc cgtgctgtct aaaatctatt
     4381 ctaatacaaa atatagtata atgcaatccc caaaacacat gataatgtat tttcagaaca
     4441 agcctgtttt tatgaaagat tgtcgttttt aagggaatat ttccgtttcc ttttacacaa
     4501 ataaacttca caaataaaat tatctgtcac tttcccgcaa tccgaatccc aaacctgtta
     4561 aatccgacag atttcaagca aatataaaaa atcatcttat cgctcacaaa acaatcgctc
     4621 atcataatat aaaaagatat caaaaataag accgcctgta gcggaaaagc cattttccca
     4681 ctgcagacag ccttatatac tgtacagatc tttttgtatt tacaatcttt attatgtaat
     4741 cttatctgct tatttgcttt gctttttttg tcttgcgaaa taatctttcg tctctcttac
     4801 gacaacaccg cttaacgcca aaatcgcgat caagttcgga aacgccatca gcgcattaaa
     4861 aatatccgcg atattccaaa cggccgcaac cgtcatatat ggtccgataa atactgcaag
     4921 aatatacaac cagcggtacc cttttactac ggatttattt ctgttaaaaa gatattcgat
     4981 acatctctca ccataatagt tccatccaag tattgtagtg aacgcaaaaa atacaaggca
     5041 gagcatcagt gagaacgaag ctacgatcgg agggaacggc aggccttttt ggaatgccgc
     5101 caccgtaatt tcaacgcctt ccagttccgg gttcatccat gtacccgcta tcacaatgga
     5161 aaggcctgtc attgtacaaa tcactatcgt atcgataaat gttcctgtca tagacacaag
     5221 tccctgacga accggctctt ttgtctttgc cgccgctgct gcgatcggcg cacttccaag
     5281 accggattca tttgagaaaa taccacgggc gattcctttt tgcatcgcaa cgaccattgt
     5341 tcccattgcc ccgcctgcca aagcgctgcc cgtaaaagct gattttataa tagatacaag
     5401 cgctgccgga acagccgtaa tatttgtaat aataatgatc gctccaagcg ccacataaag
     5461 cactgccata aacggaacga caatttccga tactttcgcg attcttttaa ttccgccgat
     5521 cacgacaagg ccgacacaaa gtgtcaagat caacccagtc acaaccgtag ccatcgtata
     5581 gtcatgacca aaggcagaaa aaacaacgtt tgatgactct ggatcaaaga agtttttcac
     5641 cgctgacgaa atactgttta cctgcgtaaa agtaccgata ccgaaaaggc cgacaccggc
     5701 tccgaaaaat gcaaagattt tagccagcca tctccagttc tttcccattc cgttttcaat
     5761 atagtaaaac ggtcctccga gaacatgtcc gtctttgtcg atcgtgcgat atttgatagc
     5821 cagaagacct tcggcatatt ttgtagccat tccgaaaaac gccgcgataa ccatccaaaa
     5881 cagcgctccc ggacctcctg ctgccaatgc agttgcgact cctgtgatgt ttcccgttcc
     5941 gatcgttgcc gataacgccg tacaaagcgc tccgaaactg gttacttcac cctgtccgtc
     6001 ctcttcattt ttaaacataa actttagggc tttcccgaga tgtcttattt gtaaaagacc
     6061 cagccgggtt gtgagaagaa atcctgtaaa caggatcaga atgatcaacg gcagacccca
     6121 caccgctccg tcgatcacct taatgatttg gtcgatttgc tctaacattc ctttatacct
     6181 ccatcctttt gcctgagaga ctcgcggcgt ctgccttaaa aacagaatat accgcttaca
     6241 ccttcggcgc tctttcaccg gcctcgccac agccgataaa gagactctcc tgagttttct
     6301 ctttcgtgct ttaacacgac atagttcaga ataacatgga aagtataaaa aagcaagaaa
     6361 aatcgcccga acctcaaacg atccgagcga tttttctatt tataaaggct tttgcaacct
     6421 tcttttatac cgtgccttgc gatcgacaca attactgacg acgtcttttt ctctcaaaaa
     6481 atgccgctgc cgctgcaaat aatccgaaga tacctgccgc tccccatcca aagaaggatg
     6541 tcgcgtctcc cgtctgaaca gcctgacctt tattcggtgc acccgggttc ttattgtccg
     6601 ttgttcctgg gttcttattg tccgttgttc ccggtttctt caaaccatcc atcgctgtct
     6661 gaagacttgt ctgtgccgcg tcaacttccg cctgacttgc atcttctttt gcaagaacag
     6721 cctgcgcttc ttttaacgcc tctgcaaacg gcttccatgt ttcagatgta taatctttct
     6781 ccttcaaagc ctctgctttt tcaactaatg ccgtcaagtc agctttattt acttcaactt
     6841 cttcgtcttg gaatcttccc atcacacgga tctctgccgc tgccgagaat ttatttgcct
     6901 catctcctgt agtcgcagtc ggtacgagtt ttacgtctgt tacatctttc acttcttcaa
     6961 aagcaaccgc ctgccatccg gttcctccga agcttgtatc cgctactttt tcgtagtcgg
     7021 cagcgcctgc ttttttcacc cagatttccg cttccctgat aataccgttt cttccgcttc
     7081 tctgctgaag tcttaatccg cttactgtcg ccggtttatc taattgcaga tttaaccagt
     7141 ggttttcaat cggtgttcct gcccatacag tatgccaaat tgtatcggca ttaccgtcta
     7201 agacgttgct tgcaggtcct tctccgcctt cctgcgcctg ctcgcttcct gctgtagcag
     7261 tcatctgctc tttcggaaga tctttggaat catctgtcgc atcgattcca gccgagttat
     7321 ccatcgtaac catagcagct tttaattcat agtatgcttt ctcgatttca tcctgtgaag
     7381 ctccctcttt gtcaagaacc gcttttgctt ctttaagtac gtcctggaag ctgctccaaa
     7441 tttccggtct gtaatcgatt tctttatact tttcttctgc ttctgcaact aatgcggcaa
     7501 gtttttcctt tgtaacttct tctgttactt tttcgatcac gaggttgtca agaacaagct
     7561 ctttgtatcc gccgaagttc gcctctgccg catccggact cacgccttga agatccggag
     7621 ccttctctgt agagtagata ccgaaccatg tctgtcctgt ggaatctccc gtaacctgca
     7681 ttgtaaaatg tccgtctttc tcttttccca tgctcatcgg aagttctttg agctgaacgt
     7741 ttccgttata ctcgccgaca ccgactgcag ccgcgtaaat tccgtcgctg cccgcctgat
     7801 aatcaaagct tacattgtat gtcacaccag gctcgaaacg gaagttctgc gggatcgtct
     7861 ggtatgccag cgtagatctc tgtgtcagtc cgttgatctt cacagaccag tctccgtcaa
     7921 gaacatcatc catcttcttc acatcccatc ctgcctgtgt gtacttgtca tgtctttcag
     7981 aaagatggat tctgttatcc tctactcctt caattcctcc gactacgaac gggtaaagtc
     8041 cctgtacgct cttttcaaaa ttctgttcaa attttacaac ttcgcccttc tcgtttgtcg
     8101 taatgttctt agaatcgttt tgcacgatac ggatatcatc gaagtatgaa gatccttctc
     8161 ctgcttccct tgcgatcgta agagtcacat ctcctgattt cggcgctgta aagaatatat
     8221 acatattctg gaaatagctg cttccgtcta ctgtcgaact gtttgtatta tgtgtatatg
     8281 cttttacata gttcttagcg atagaacgtg ttgtgtagtt tgatccaagt actttatcgc
     8341 ctgatttaat ttctacagat gctttggcgt cgcttctgtt atctacgcct actaaaacag
     8401 catactgttt tcctgcttcc agatctgtca gtttctgtgt catagaaact tttccgtcca
     8461 gtttcatcat cggattgctg tgctggctct ttgcgatctg cgctgttcct tcgcctgctt
     8521 ttgtccacat atcaaggctt ccactgttga atcctgcatc tacgatatgc attccttcac
     8581 tccatgtcac tttgatattt tcttttttgc ctttgcatac tacatacgga acttctgatt
     8641 ctgcttctaa tgtgatctta ccgtcaacaa cattgaccgt cttctcctct gtctttccga
     8701 gatctgtcag tttgtatact ttgacgtctt ttaagtctgc ccaactgtcc ggaagctccc
     8761 actgtgttgt tccgccctgc gtattccagt ggtacagctt ttcatcttct gattttactt
     8821 tttctcctgt cttcgcatcc cagatccacg gcagcagata agactctgtt ccttttttgt
     8881 ttccgttgcg gatatcgtcg tctgttctgt ctccctgtga taccgcgcct cttgcgatca
     8941 cttttccgtc caaagtcatc gttctgtctc tgtatgccgc ctgagccgga tcgtttgatc
     9001 ccctctcaag aacaagcgta gatccatctt catctttcaa agtgatcttc atttcaggcg
     9061 tccagtttac cgctccgcct acgttgaccg gtgttccgtc ttcccaatct acgatctgat
     9121 aatgctggat gaattttgtc gtcaggtcat gtgtgtaaag attcgtaata tacgcgtcat
     9181 aatcgttacg tccctgccat ccttcaaagt ctttcatgtt atatccgcca agaagaggag
     9241 caaccgccgc gcctccgtaa gtcggataat ctcctaccca tgaatctttc tggtggttac
     9301 gcaggaaacg catcacatca ctgttctctc cttttgcgcc ttccccgcca tatgtaaggt
     9361 ctgttgccca atgctggaat gtagagtcat actcgtttgc tccaccccat tcgttggaca
     9421 tacgccatcc gttgtcatta atctctttgg acagcttacg ggtctgctgg gaatcatcat
     9481 tgtcacttcc cgtattattt ccccaaatat ctacatagat gaagtcaagc atatcttttc
     9541 cgtcgccgcc gaggatctca tgaagttcat caaagcgcgc ttctctttct cctgtggcaa
     9601 ggtcatagat actgtcaagt ccgattccct gatcgatcca gttccatcca taacgaagac
     9661 ttccgtcctt attacggcga acattatcat ccttaaacgc ttttgcttcc ggatacatct
     9721 cgcctgcatt gacatggata ccgaattttg ctccgtaatc cgcgcctttt tcaagcaatg
     9781 tcttcatatc ttccggtccg ccgattctct ttcctatatc tgcgtaatcc ggatgcgccg
     9841 agtcatgtcc ttcattcgca taacctttca gcaggacaga ttgtccaaga ccgtctgtgt
     9901 gcattgccac acgttttacg ttatctaatg ttgtcaggaa cggattctgc gcgtgagaac
     9961 cgaagttcat cgcaatacgg tacgcaacca attccggaac ttcctcgctc ttgaacggat
    10021 tgttcataat atcacggaat gcaaccgctc cgtcctgcca gtcaacattt ttatcgccgt
    10081 tgcagtttcc tgtgatcacg actttcatct tcggattttc tgtctcttcc aaaacccatg
    10141 tccgattatg agaatctgtc atcactctgt gccagtacca tgcagaactt ccaagtccca
    10201 tcgatacata gccgtctttt ttctctgaga cagacatcac tcttgtatta ttagatccgc
    10261 cggaagaact tgctccggca ttgcgacctt caaactctga attgctccaa agtcctgcgc
    10321 tcatctcatc gttagaaaca aaggcataca tgtagtctct ctctctggca ggcgtatttg
    10381 catttacttc tacatattcg tcaccgctta cctttgtttg tgtggacata gcagcgccta
    10441 ttaaattggc atttttctgt gtactgttta cagatacaag actgtgattc gggatctcga
    10501 tcgtctggat cggatttccc accttgccgc tttccaatgc tgtgcccggt tttccttctg
    10561 tcagcttatt ttccactttc gtaatttcaa atgaaaccgt attgtctttt gctgtcaatt
    10621 ctgccgtaag aactgcgtca atatgttttt cttcattttt cacagtcatc tcatacgttg
    10681 ctttgtcacc cttgatcgtt gcttttacat cacctttgga aagtttcaca tctgttccgt
    10741 tgatacgcac cgtattgata gccgaagtct gaccgtagaa cgtcttgccg tttaaatcgc
    10801 cctttttcat ctcgtatttt acaacgctcg ggaaattctt cgctacataa acgtccatat
    10861 ccgctgtaga aagcttctct gtctctatct ctgctgtctt ttccagtttt acggcttttg
    10921 cttccacgtt gccttctgtt acagttacag atgtcgtcgc cgtctgatat cctgccttcg
    10981 taatcgtcag ctcatgcttg ccggctccta actgtaagga atattttccg tctttatccg
    11041 tttcagccgt cagatttcct gtcgttacaa ccgcgccttc caacgctttt ccactctcat
    11101 ctgttacact tcccgtaaca gtatatgtaa ccgcttcttc ctgtcctgta taatgaatat
    11161 ctttcaaaag gacatctgaa ccgatctgtc cccaagaacc tgctttgatc gcaattttgt
    11221 tcccgagaga atctgcgata ccggaaaagt cttctttatc aaacacaact tctccgttga
    11281 gcgtaaacgt catcttatga tctgctgtcc agtcgatgcg aacctttact tcctgatctt
    11341 tcgtcggcgc aggttttctc gtctgctgat aatagtctcc gttgccgcct ttgtatttct
    11401 gccagaacca gccgccattg tcatatccta tgtacatacc gttttgatcc gtattatatc
    11461 cgaggtaaac gcctactctg tcactgtcgc ttattcctgt atttgcatta tttgatttta
    11521 aagtaaatga aaaatatccc ggcttgttaa aatcaaatgt atttggattt acaaccatct
    11581 gcggtcttgt tccgggatta ttccgattgc ccgcagctga tttaatatgc agccagccat
    11641 tttccacagt gactgtgtct ccgtctgctt tgttatcctg agtccatgca tcattgatcc
    11701 catcttctaa agctgctgtt ttttctgtaa catcaacttc ttcggcagat gcggtctgaa
    11761 tgtttgacgg aagcatagta cctgtcatag atgcaaccat cacaagactc aagagtctta
    11821 agactacttt tcttttcata ccttttctcc ttcccttcag gctgatttta tatttcagcc
    11881 aatatatcac atcctccatt atagcaaatt accaattttt tacaatgtat tattcatatt
    11941 tttagcgttt ttttgtctat ttcttatctc ccttactttt ccccgtcgtt ttttctttgt
    12001 ttttttgtat tttattattc tttttgagca ttttctctca cttcttccaa catctcagcc
    12061 actgtttttc catacgccgg atggcgcaca tacggcgcga tctcaaaaag aggccgcagc
    12121 acaaaatctc tcttgtgcat ttccacatga gggatgcaca actcgtctcc ttcatatacg
    12181 agatcatcat acaaaataat gtccaaatca agcgttctcg gaccccagtg aacgatccgt
    12241 tctcttcccg cctccatttc aatcctattt aattctctta acagttcttt cggataaaac
    12301 aaggttctta atttcagaca tccgttcaga aaatcatcct gatctgtcac cccatacgga
    12361 ggcgtttcga taaaagaaga gcatttttcg acaacgcatc cttttaaagc gccaagcgct
    12421 tcaactgcat ctgtaatata tttttctctg tctcccatgt tagatccaag tgcaatatat
    12481 gcggtatgcc attgccgctc gatcacaacg ctgaccgttt tcagcggaag gcgcaccgga
    12541 gcccacggtt tcttgatctc cagtctgatc ttctctagtc cggctgtatg cagcaaaagt
    12601 tcttctgcaa gcgcttctgc cgccctctcc aataattgaa atgtatgctc ccgcaagtag
    12661 cggtctataa aagcgctcac ttccccataa tgaatagaag cgcttagatc atccgtaagt
    12721 cccgcccttc gtgtgtcggt gtaaagaact gccgagacta gaaacttttg tcccaacaca
    12781 ttttcctccg gaaatacacc gtgattcgca aatacttcca gatcttctat tctgatctca
    12841 tcataatctt ttttcatttt ctgtcttcct tcctccttac cgtacatttt tctgtatgac
    12901 aactctttct tcaccttaac tatacgaaag cgaagcagtc agtccgacag cttcgcttgc
    12961 ctgttttaca gtcctgatta cacttcatgt tcccgcatga gcgcctgcgt cataagtatc
    13021 gctcttttat tcttctctac gtcatgaaca cggacaaacg cacatcgttt ctgcaccgca
    13081 taaaccgttg tcacaagcgt tccttcttcc cgctcgtctg ccggaagatc taatgtaaga
    13141 ccgatcacag atttgcgtga cgttccaaga aggatcggta atttaaattc atgcatgatc
    13201 tccagtttct gtatgatctc cagattcatt tcatatgtct ttccaaaacc gactcccgga
    13261 tcaaggatga tcttatcccg tgcaattcct gctttatctg caattttaac gcactctttc
    13321 atatctttca taaaatcgaa aagaaaacta tcgtataccg cctcgttccg attatgcatc
    13381 agacagcatg caacgtcatg tttcgcgatc acatctgcca ttttctcgtc atattttaat
    13441 ccccatatat catttacaag atctgccccc gcacaaaccg caacttcagc caccgcgctt
    13501 ttatatgtat caatagagat cggtacgtca aattcctttt tcagtttttc aatgaccgga
    13561 acgacgcgcg cgatctcttc ttcgtccgta atctgaacat gtcccggacg ggtagactca
    13621 ccgccgacat ctaaaatatc cgctccttct tttatcatct tttctgcatg aagaagggct
    13681 gcatccatct tatcaaaacg tcctccatcc gaaaaagaat ccggagttac atttaagatg
    13741 cccataatat atgtattgtg ttctgtgtca aattctcttc tgcctatgat catattttta
    13801 tctccatatt tttcttctct tcactccagt tacattcccg gtttgccgga catgaaagac
    13861 acagcctcga cgctttgtcc gcttcataca aaagcttttc caatgtgtcc tccccgtcac
    13921 gaagccgtct gtgtccgcga accgcatttt ttatcgttct tttttcttcc tcagaaaatc
    13981 ccgtttcttt cggaagtgta tccagtattt ccccggcgat ccgctctccc gcaatctcat
    14041 ggggaacttt ttcttcatat tgaagcgctt ttcctatatc gtgcaaaacc gcggcggcat
    14101 aaattacatc tttacgaaaa ccgtatccct tctctaaatt acggatatat gctattcttg
    14161 ccacatccaa cagatgcgac atctgatgcc tgcaaaaaat acgttccttt tccgcgcgct
    14221 ccagcctatg atagtattcc aaatacagcg gatggcaaat cactgcattt actctgtcca
    14281 tttttcctcc tttaccggtc agtttcccgc gtccggtcta ccctctttca agcattctga
    14341 aaaaacgttc ttccaaagcc ggatctgtct caaacagccc tctttttgca aatgtcaccg
    14401 tcctgctgcc cggttttttt attcctctca tcgtcataca catatgttct gcctcgatca
    14461 caaccgccgc gcctttcgcc ttcagttcgt tcatcagcgc atctgcgatc tgacccgtaa
    14521 gctgttcttg aagctgaagc cttcttgcaa acacctctac cgttctcgcc agcttgctaa
    14581 gccccgcaac ttttccgtcc ggaatatacg caatgtgcgc tttgccgtaa aaaggaagaa
    14641 ggtgatgctc gcacatggaa tagaacgtaa tatctttttc tacgaccata tcgctgtttt
    14701 ccacatgaaa cgtcttacaa agatgctctt ttgcatcttc ttcgtaacct ccgtatattt
    14761 cctcgcacat tctggcgatc ctatccggtg tttcgatcaa gccttctctg tcggcgtctt
    14821 ctccgatccc ctccaataat aatctgactg ctttctttac tttttcctga tctatcatct
    14881 tttcatcctt tatctttttc tatcaataca tctgtcgctt atcctgttct ttttcaaccg
    14941 cttcctttac ttttcccaaa taggacaaag agccaaacgc aaggatcacg tcgtcttcgt
    15001 ctgcgttttc cagcgcttct tttaccgcct gcttaatctc gccgacagca tacgcgggca
    15061 catattcaga ctgtgcttca acagcttctt ttagtttctg cgcgcttagc gtacgttctt
    15121 tattcggaag atcgaccgta tatacgcatt ttgcatacgg aatcgtcaaa cggatgatct
    15181 tttcatattc tttatcttga aacacaccca ttatgtaaat aagcttcctt cccgggaaat
    15241 acgcttctat tgattcccgc agtctttttg ccgcatcttc attgtgcgct ccgtctacga
    15301 gaaatacagg ccgctccgaa atacatgtaa accttccgaa ccattctgta tcgaaaaaac
    15361 ccgtctcgac cgctctcttt tcctcttttt tcagcagtcc cggaacatgt actgcctccc
    15421 atgcggtaac agcgtttatg agctgatatt ttcccgaaag acggatgctt atattttcgg
    15481 aatctttata agaaaaccgc atcccgcgat aatcttcttc caagacaact gctttttccg
    15541 gctctgcata aataatcgga cagccaaact gtcttgcttt ttgttctaaa atttctctta
    15601 cttcctgctt ctggggcgct gatacaacga tgcatcccgg ttttatgatc cccgccttat
    15661 tttcggcgat ctcggaaagt gtattgccga tcacaccgat gtgatccata ctgatcggcg
    15721 aaaacacggc gcagactgta tttttcacaa cattcgtagc gtcttccatc cctcccagcc
    15781 ctgattctaa aacgacaaaa tcgcacttca ttcttttata atataaaaat gctatcgccg
    15841 tttcagcctc aaagactgtc ggaagctgtt ttcccgcttc ctttaaccga acgatagcat
    15901 cctgaacttt ttctgtctct tctgcaaaag catgctttga tatcatttct ccgtcaactt
    15961 gtatcctctc acggtatgag acaactgtcg gtgatacata acgcccgatc ctgtatcctg
    16021 cttcacttaa tattgtggaa gtgtaggcaa gcacagatcc ctttccattc gttcctgcga
    16081 tatgtatgaa ccttaaatca tcctgaggat tcccaagttc acgcagcaga cctcgaattg
    16141 catctaagcc aagtacactt ccgtatttag acaattcatc taaatatacc ctcgcttctt
    16201 tataggtcac acttttgttc ctttcctgaa tcaatttcat tttccgccct gctttttgcc
    16261 gagcagactt atcatagcac tattcgtttc attgtacaag cttcatattt gcaacaattc
    16321 ttactttgta tagacatttg ctcctttctg tgttacgata agtgtactac attaaataat
    16381 acacttaata cactcatttt aaggaaggga caattatgat tacgattgac tatcaaagca
    16441 aactgccttt atatgaacag attgctaaaa gatttcagac gctcatctta aaaggcgcgc
    16501 ttccacccga ttcgcagatg ccttccgttc gttccctcgc aatggatctt tccatcaatc
    16561 cgaatacgat acagaaagca tattcgcttt tggaacagga acgctatatt tatccggtaa
    16621 aaggacgcgg aaatttcgtt tccgatacga cgcttctcgt tgaacagaca aagaaaacac
    16681 ttctcgagaa cgtttctcgc cttatcgcaa acggaaaaga actcggagta aagcgcgcag
    16741 aattcacgca gcttgttgac cggatctatc gaaaggagga ttctcatgat cgagttaaaa
    16801 acgcttgata aaacatttca gatgatccat gcttccgacc acatcaccgc tactatcccg
    16861 gacggtatgg tattcggact gatcggatca aacggagccg gaaaaagtac gcttctgcgt
    16921 atgatatccg gtattctcaa accggacgaa ggagaagttc tcattgacgg agaatctgtt
    16981 tttgaaaatc cttccgtaaa atcacagatc tgctttttat cggacacccc ttacttcttt
    17041 ccgaatgcag atatccaaca gatgagaaat tattacatgc tctgttaccc gtcgtttaac
    17101 agaaaactgt ttgactctct tacagacaga tttaatcttg atccaaaacg aaagatcagc
    17161 agcttttcaa aaggtatgaa aaagcaagtt tccattttgc tcggtctttg ttcaggaaca
    17221 aaatatctcc tctgtgacga aacgttcgac ggacttgatc cggtcgttcg ccaggctgtt
    17281 aaaagtcttt ttgccgcaga gatattgaat cgggatttca caccgatcat ttcttctcat
    17341 aatctgcggg agttagaaga cttctgcgac agcatcggac ttttgcatca gggacagctt
    17401 ctgcttaccc aaagtctgga tcagataaag tgttccgtct gcaaactgca gtgtgtcatt
    17461 tcagatccac ataaagaaca gcagtttctg tcttctataa agatattaaa aatggagcgt
    17521 tccggttctt tacttacgct cacacttcgc ggaagccact cagatattct ttctgcggta
    17581 cagacttatg atcctgtttt tgcagaagtc cttccgctta cactcgaaga aatatttata
    17641 agcgaaacgg aggcagccgg ttatgatatc aaagatttct tatattaaac ttatacgaga
    17701 ggacatacgg cacagaggat ggctcgccgc cctttccggc gtcctgttat ttctttcaat
    17761 gcctgtttac acactgatct accttgacag ttattctgaa gatcctgaag tattaaaaga
    17821 gttcgcggat acattttccg gattcattaa cggatataat tccgcttttc tcatcatact
    17881 gatcggaatt ctcgctttcg cagtcggaat aagcgggttt cagttcatgc ataaaaaaga
    17941 aaaacttgat ttttaccacg gacttgcgct caaacgtcat cagttgttcg caatctctta
    18001 tttggcagga ctgttcctct ttctcatccc atatatagta tgtactgtat tgacgatcct
    18061 tgtcggcgct gtaaaaggaa ttatgactct ttctctcgcc ggagaatgtt ttcttacaat
    18121 ggggacggga atcctcgcat tccttctgat ctatcacaca tgtatttttg cgggaatgct
    18181 gaccggacga agcgtaacga tgttcctcgg aacacttgtc atcggagtct atccaagcct
    18241 cgtattgaca ctgcttccgt cgctgcgaga tgcgtttttc cagacaactt ataacgtaca
    18301 cgacagtata cctgaaaaac tttcggaata tctttcgcca tttccgttgt tcatccgcat
    18361 gcttgaacaa aataattctt actcgaatgt gtttatgatc accgctgctc ttgttttgat
    18421 cgttcttctt ttggcggcct cgatcatgct gtatcgcatt tacccttcgg aatcagccgg
    18481 aaatgccctt gcgtttaaat ttacaccgcc tgtatttaaa gtactgatca gtattccgac
    18541 cgcagtgttt atcagtcttc ttgtaaaatc atttatcgga gcttccggca caaaatggat
    18601 cattatctta agtatgctcg ccattgtcct gctatgcgga ttgatcgagt ttatttactg
    18661 gcaggatctg aaaatgctat taaaaggatg gcgttcttcc gtaattgcca tcgggtttgt
    18721 agccgttttg cttgcagtgt ttcaatttga ttttaccgga tatgacacat accttccgaa
    18781 agaagaaaaa attgcccgga tcggaacgct tcccgattcg ttcgcatcct atttctggta
    18841 tcccgaagac tatacttcag aggatgacag ggaaagtttc gttctttccg atgacgttga
    18901 cgttttatac aatcttgcgc aatcgggaat cgaaaacgtg aaaaaaggca ttactccaaa
    18961 cacgctttac tcctctcaga catcgatgca gacggataaa gattccgaca attatatatg
    19021 cgccgcattt tgctttgaac tggaaagcgg gaagaaagtt tatagaaact actgtgtttt
    19081 ctataatgaa acgcttgagg cattcacaaa gctctgccaa aaagaagatt accgcaagga
    19141 acttttcccg atcttccatg tggagaaaga aaacgtatct tctgtctcgc ttcaggatat
    19201 ttatataacg cccgaagaat tacttctgac tgccgaacaa agaacagaac ttctaagcgc
    19261 atatgaaaaa gacgtattaa acacagatat ccgaaaactc ggaagcgaag ttccgattgg
    19321 agaattttcc cttacgatcc gccatgttcc taacaaagag acgaatacga atcagccttt
    19381 ccattcatgg gtgaacggaa cgcgtgaatg gaatgctgca gtcaatacag cttttccggt
    19441 aacttcctcc gaagatgaaa ccaattacaa gacgttttcg atcggatcat tgtacattta
    19501 cgaagactat gaaaatacac tttcctgtct tgaaaaatac ggatacaaaa taaaaagact
    19561 tatccgtgca gaagatatcg actctgtcac tctatactta agcggcgact caatgaaaaa
    19621 caaaaaatgg aacgatcttt tagcatcctt ttcttctgcc aaagatgaga acaattccga
    19681 tccggataaa gctgcttacg aacaatcaga tgaaaaagat gcaacgtcaa tgttcacgat
    19741 cacagctccc gaagatttcc ggcttctggc agaacatctt taccttaatc cttctgattt
    19801 tctcgacagg ggaggaacct cacgtgatta tgcagaagtt acatttaaaa aagaaaacaa
    19861 tactttcact tatcttttga agtaaacatt tttgtctgat atttctgaca aaaccggcat
    19921 tgcatccaac agcagactct tttcttcatc tgcccacgga agcaatgccg gttttttctt
    19981 ttattctttt tatttcctgc t
//
LOCUS       DBMDXF010000008        19037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_893_length_19037_cov_2.100074, whole genome shotgun sequence.
ACCESSION   DBMDXF010000008 DBMDXF010000000
VERSION     DBMDXF010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 19037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19037
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_893_length_19037_cov_2.100074"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="ACZPD4_00695"
     CDS             <1..427
                     /locus_tag="ACZPD4_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HIR1377630.1"
                     /translation="ASIVLGIMILQTIISVSESAIRAVPESYYEGSLALGASHERSVF
                     RVVLPAAASGVMAGVILGVGRAIGETMAVIMVAGNQAALPRGLFSGVRTMTANIVLEM
                     GYAADLHREALIATAVVLFVFILIINVSFSVLKRRSENK"
     gene            493..1317
                     /gene="pstA"
                     /locus_tag="ACZPD4_00700"
     CDS             493..1317
                     /gene="pstA"
                     /locus_tag="ACZPD4_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1377631.1"
                     /translation="MNKSKLQSVILRTLVYTAAFITFGVLICLIAYILIKGIPNLSLD
                     LFSFEYNSENVSLFPALINTVVMTALSLVIAVPLGVFSAIYLVEYAKRGNKIVGLIRL
                     TAETLSGIPSIVYGLFGFLLFVTAFSWGYSLLAGAFTLVIMILPVIMRTTEEALKAVP
                     DTFREGSFGLGAGKLRTVFKIVLPSAVPGILAGVVLSVGRIVGETAALIYTAGTVAQI
                     PSGLFGSGRTLAVHMYALWSEGLNTNQSYATAVVLLIIVVILNAASSALAKKISSK"
     gene            1373..>1729
                     /locus_tag="ACZPD4_00705"
     CDS             1373..>1729
                     /locus_tag="ACZPD4_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR1377632.1"
                     /translation="MSVISIKDLNLHYGDFHALKSVNMEIPKNEITAFIGPSGCGKST
                     LLKTLNRMNDLVEGCRIDGEVKLDGINIYKDMDTNLLRKRVGMVFQKPNPFPMSIYDN
                     IAYGPRTHGIKSKAKLD"
     assembly_gap    1730..1739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1740..2125
                     /locus_tag="ACZPD4_00710"
     CDS             <1740..2125
                     /locus_tag="ACZPD4_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR1377633.1"
                     /translation="QKSLERAAIWNEVKDRLKKSALGLSGGQQQRLCIARALAVEPEV
                     LLMDEPTSALDPISTSKIEDLALELKKRYTIVIVTHSMQQATRISDKTAFFLLGEVVE
                     FGDTEQIFSIPKDKRTEDYITGRFG"
     gene            2129..2797
                     /gene="phoU"
                     /locus_tag="ACZPD4_00715"
     CDS             2129..2797
                     /gene="phoU"
                     /locus_tag="ACZPD4_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1377634.1"
                     /translation="MRNRFDRQLHLLDEQLTHMGELCETAIAKATQALKDGSIEQAKV
                     IIAEDEEIDQIEKDIERLCLKLLLQQQPVAKDLRRISAALKMITDMERIGDQTSDIAE
                     IILSSKGVAATDEIHVIGTMASEVSKMVRTSVTAYVEKDLELARSVMKADDEIDQYFE
                     EVRDEMIEFIREEKGENGKRIFDLIMVTKYLERIGDHATNIAEWVEFSITGVHKDHAI
                     IHAE"
     gene            2829..3503
                     /locus_tag="ACZPD4_00720"
     CDS             2829..3503
                     /locus_tag="ACZPD4_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1377635.1"
                     /translation="MIYCVEDDDNIRELVIYTLETTGLKARGFADGSAFTEALAFETP
                     ELILLDIMLPGEDGLEILKKLKCSSKTKSIPVIMVTAKGSEYDKVIGLDSGADDYVTK
                     PFGMMELVSRIKAVLRRSKGSQERENPEIQGVHIDVKKHEVTVDGRTVPLTLKEFELL
                     EKLMRNEGIVLTRDQLLTEIWGYDFDGETRTVDVHIRTLRQKLGEKGEIIQTVRGVGY
                     RIGGSA"
     gene            3500..4834
                     /locus_tag="ACZPD4_00725"
     CDS             3500..4834
                     /locus_tag="ACZPD4_00725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1377636.1"
                     /translation="MSIKKKIQQSMTVILTVTLVLAYVILSVILYNRNMNLLQTEVKQ
                     EAAYIREAINLSDSDYMKQMDNVVESTRVTQIKADGTVLYDSRRDADILKNHGNRKEV
                     REALSKGEGADVRKSETVGKDMYYYALLLNDGTVLRVSKSIDNLASTAVSLMPVMGIL
                     AVLTILFAFALARWQTNRLIKPINELDLERPLDSDIYEELSPLLEAMDRQNREKEAVA
                     NMRKEFSANVSHELKTPLTSISGYAEIMKNGLVRPEDISGFSERIYKEARRLITLIED
                     IIKLSKLDEESVELEKEDVDLYELTREIVSRLSPQAAQKNIRIELSGESVHYHGIRQI
                     LDEMVYNVCENAVKYNYENGKVSVWAGNTLNGPKISVSDTGIGIPKEHQERIFERFYR
                     VDKSHSKERGGTGLGLSIVKHGALLHGAKVSVESEEGRGTKISMQFPRICQK"
     gene            4991..5359
                     /locus_tag="ACZPD4_00730"
     CDS             4991..5359
                     /locus_tag="ACZPD4_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1377637.1"
                     /translation="MIIEVDFNSDEAIYIQLCNQIIMGIATSVIHEGDSLPSVRRLAD
                     TIGVNMHTVNKAYSVLKREGYISLDKRRGAVISLDIDKLEELQVMKRQLQIVLARGCC
                     KNISRQEVHELVDEIFDDYE"
     gene            5406..7850
                     /locus_tag="ACZPD4_00735"
     CDS             5406..7850
                     /locus_tag="ACZPD4_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1377638.1"
                     /translation="MHISYTKQAANAVRYAAKKAKEMKHPYIGTEHLLLGLREEFSGV
                     AGQVLAQNGVETEKIMQLMDELIAPREEMPASQKPKESPRLRYILANSEKEAHRLRTA
                     EVGTEHLLLSMIRDVDCVAARILITLNISLQKLLKDILNASGVDPKDYQDELQDESRG
                     SGSVIEQYCTDMTARAEEGKQDPVVGREEEMYRLMQVLSRRTKNNPCLIGEPGVGKTA
                     VVEGLAQRIAAGVVPEKMKDKRIYTLDLPGMIAGSKYRGEFEERMKGLISEVESNGNI
                     ILFLDEIHTMIGAGGAEGAIDASGILKPSLARGELQLIGATTITEYRKYIEKDAALER
                     RFQPVSVEEPSKEQCLEILKGLKGRYESHHKVLIRDEALEAAVSMSERYITDRNLPDK
                     AIDVLDESCSKVSLKGYKVPENLTALDLRLKELEKQKEESIKNGCFEEASLLQKEQEE
                     AEKKSEQLKKRFQKKTSSSQPEVTEEDIAEVVSSWTKIPVQKLAESDTDRLKKLESVL
                     HQRVIGQEEAVKAVARAVKRGRVGLKDPKRPIGSFLFLGPTGVGKTELSKALAEAMFG
                     NEESMIRVDMSEYMEKHSVSKMIGSPPGYVGHEEGGQLSDQVRTHPYSVLLFDEIEKA
                     HPDVFNILLQVLDDGHITDSKGRKIDFSNTVIIMTSNAGAKAIIEPKKLGFAAKDDPA
                     GDYKRMKQNVMDEVKQIFRPEFLNRIDEIIVFHALEKTHMKKIVTLMCRDFTKRIEDQ
                     MDIRLTLRESAKALIAEKGTDAKYGARPLRRALQTELEDKLAEAILNGEVKRGDCIEA
                     GTVKKEIRFIRKEDRL"
     gene            7973..8386
                     /locus_tag="ACZPD4_00740"
     CDS             7973..8386
                     /locus_tag="ACZPD4_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HIR1377639.1"
                     /translation="MAAVKELLRAENDGSLSFGDYTLSEKTKKDNYEFEGDIYKVKTF
                     SEITKLERNGMFVYESVPGSAVEGFKETDSEVAFSVSAKGDVQFTLELEPECEYEVFI
                     DGESAGKMRTNLSGKLSVSVELSEGQEATMRVVKC"
     gene            8560..9936
                     /gene="radA"
                     /locus_tag="ACZPD4_00745"
     CDS             8560..9936
                     /gene="radA"
                     /locus_tag="ACZPD4_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1377640.1"
                     /translation="MAKGKKSVFFCQNCGHEEAKWLGQCPGCGEWNTFVEEKIGSGVT
                     KGSTQGAKAVRELVREAKVIPLNDVTAEDDVRCATGIKELDRVLGGGIVPGSLVLVGG
                     DPGIGKSTLLLQVCRILADKKKVLYISGEESQTQIKLRANRMGNFADGLFLLCETNLE
                     IIRGILEKERPELVVIDSIQTMYSEEVSSAPGSVSQVRESTNVFMQLAKGLCIPIFIV
                     GHVTKEGTVAGPRVLEHMVDTVLYFEGDRHASYRILRAVKNRFGSTNEIGVFEMRQSG
                     LEEVENPSEYMLSGRPEQSAGSVVACSMEGTRPILIEIQALVCRSNFGMPRRTAAGTD
                     YNRVNLLMAVLEKRLGMALSNSDAYVNIAGGIRMNEPAIDLGIVMALVSSYRNRPVDE
                     KTIVFGEVGLSGEVRAVNMPEQRVAEAKKLGFETCILPQVCMKMVKDIKGIRLIGVKS
                     VNEAVGMI"
     gene            10102..10280
                     /gene="ssrS"
                     /locus_tag="ACZPD4_00750"
     ncRNA           10102..10280
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPD4_00750"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10440..11318
                     /locus_tag="ACZPD4_00755"
     CDS             10440..11318
                     /locus_tag="ACZPD4_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR1377641.1"
                     /translation="MKKKWEKIKSQIFKVIITINSHHTGAYAAQAAYFFVLSLIPIFL
                     LLLTMVRFTPITMSEVMTAVLKVFPESVAPMIRSIVSQVYFQSGTIVPVTIVVALWSA
                     GKGVLSVTSGLNSICENMETRNYFYLRIRASFYTVIFLLAIVSSLVLSVFGNRISVMI
                     YEHIPFLSKVVEFIIRIRTFVTFVVLTVFWDLVYRFLPNRRNMAKTTLRKQLPGAVFT
                     ACGWLLLSFFFSVYLDVFKGFTSMYGSLTTIILIMLWLYGCMYIILLGGEINALLEKF
                     LQKRAGNLKKKKEKEV"
     gene            11365..12522
                     /locus_tag="ACZPD4_00760"
     CDS             11365..12522
                     /locus_tag="ACZPD4_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR1377642.1"
                     /translation="MIYVVEKGDTLSGIAVKFGVPVWKIVYDNQLKDKNQIVPGQALL
                     LLEVDEGQAEESTQPELAKGKTIGGYAYPFIDKEILEQSFPALKELLVFSYGFTFEGN
                     LVPPLQDDLWMIETAWRHGAKPIMVLTPFSEGAFNNQLVKVLTENVEVQERLLEQLTA
                     EVRRRGFAGVNIDFEYVLPENKVQYAAFIGKMRELLRPEGYTVSVAVPPKVSNEQRGL
                     LVEGADYRLIGENADYVFLMTYEWGYTYGPPMAVAPLDKVRQVVEYALSQIPAEKLIL
                     GIPNYGYDWQLPYERGVTRAQTIGNVQAVDLAVENGALIRYAQTAQSPWFTYKKSGMD
                     HVVWFEDVRSIQAKWKLVQELDLAGAWYWNLMRKFRGNWLMLESPDAGRNT"
     gene            12594..13766
                     /locus_tag="ACZPD4_00765"
     CDS             12594..13766
                     /locus_tag="ACZPD4_00765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1377643.1"
                     /translation="MNGNFVYCNPTKLYFGEDSLDFLNEELPKYGKNVVLVYGAGSIK
                     ENGIYDKVINILKKNEKNIAEIAGVMPNPTLAKLYEGIKIARNHNADLLLAVGGGSVC
                     DYSKAVSVSVNCTEDPWEKYFVRFDEPDCEIVPVGCVLTMVGTGSEMNAGSVITNEKT
                     KQKIGHVFEDEKIMPRFSILNPRFTMTLPHYQMTAGIYDIFNHICEQYFSDTDDNTSD
                     YISEGLMRSLIHSSKIANKDPQDYEARSNIMWTATWALNTLVACGKSTDWMVHMLGQA
                     VGGYTNATHGMTLAAVSLPYYRHIMPYGLEKFKRFAVNVWGVDASEKEDREIAEEGLA
                     AMESWMKELGLVMNLSELGVTEEMIEGIADVTIVFDTGYKKLDRTEIIDIFRESLI"
     gene            14002..15096
                     /locus_tag="ACZPD4_00770"
     CDS             14002..15096
                     /locus_tag="ACZPD4_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR1377644.1"
                     /translation="MTGGIAKVVIFSFTERGTKQNRYIYRKLKMQGYICEGYAPAKYV
                     MGEEGGVKQLPKKKEDLIRERWGKWAFLFIGAAGIAVRYIAPYVKDKFTDSAVVVADE
                     AGQYVIPLLSGHVGGAAELADQIANLLGAVSVHTTATDVRKKFAADVFAKKNALYITD
                     REAAKRISAAVLDGELIGIYAQGDHAENAVKNSKEMFSDEVCICNTIEEVMKYRYRVI
                     LADSFPAIDENTLLLKPKNIIVGLGCRKGISEELLEKGLLEILKKNHLDMNQIEALVS
                     IDLKKEEPAVVSLAEKYKVPFLTYSAEMLNEVEEVSSASSFVKKITGIDNVCERAAVT
                     YCKKHCEYFELIAGKSIETAMTAAIARRNI"
     gene            15139..15672
                     /locus_tag="ACZPD4_00775"
     CDS             15139..15672
                     /locus_tag="ACZPD4_00775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1377645.1"
                     /translation="MVFELQFLDWLQTLQTPILNEIMKAVTHLGDAGALWIILAAVLL
                     AFPKTRKSGAIVAAALIVDLVLCNGILKNIFARTRPFDVKEGISLLITAPKDFSFPSG
                     HTAASFTAVAALYFAGEKKLWKISCAVAVLIAFSRMYLYVHYPTDILGGIIVGIVSGL
                     VGAYIIKRVQKRGVVPN"
     gene            15771..16553
                     /locus_tag="ACZPD4_00780"
     CDS             15771..16553
                     /locus_tag="ACZPD4_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377646.1"
                     /translation="MKNENQHFSADRRKFGRHFWIGAAVAGALSAAVIFTAASAVYRK
                     NRFDLKIGEEKVGSKEYQLCMDLVKYDVKMEIQKRYDALYGEDFWEQSYEGQYGYEIL
                     ADHTVEKIKYIHAVYDLAEEYGDVSDGSFAAIEKRWKQENAKRQKKVEDGEVIYGLKE
                     YTFDLYMQYEISMYKEIYCNDTDRKGMDLTEEEVAEYYSQGEWVFQDDGEKADLETAR
                     IAVERELREKKYDAMIAQMTEDLEVSGDLEAVDRFTLDHLKR"
     gene            16633..>19037
                     /locus_tag="ACZPD4_00785"
     CDS             16633..>19037
                     /locus_tag="ACZPD4_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1377647.1"
                     /translation="MKKKWVSGMLALLLVGTTVGSMMPTEAVQAEENSQGKTYYVDSE
                     NGKDTNDGLSEGKAFQTLNKVNDLTLGAGDRVLLKNGSVFEDQALHIKGSGSENAPIK
                     ISTYGDEKDGRPQINTNGHGQWELNYGHKLDNQNHKWHGTVSSSILLKDVEYIEIEGL
                     EITNDRDSATDAEKDKNYKYNDAECMDRTGVAGVAKNKGTVDHIVLNDLYIHDVTGNV
                     YNKHMTNGGIYFIVEKPENESATGIARYNDVTIQNCYLDTVNRWGIAVGYTYEWAKFN
                     GGALSDETMKTYASSDVVIQNNYLNNVGGDAITTMYIDRPVIQYNVSENAAAQINTTD
                     YTDPQPQLDANGNPNGKTHTGGRVAAGIWPWKCKNAVFQYNECFKTLNASKGNGDGQP
                     WDADYGDGTNYQYNYSHGNTASTIMFCGGESINNTFRYNISVHEDMGPLDPAGNAGNT
                     QVYNNTFVIKEGIRSIWYRDSGPVTMENNIFYFDGEQPAQITNWNPRNNKVYSNNLFY
                     NVSSYPDDKAAVKVEKGTPVLADAASGPVKAAEDKQARRHEDPTEITVFDGFKLAENS
                     PAINKGKVVIDRNGYSIDHDFFGHAVTATPEIGAAESDVIGDLVLRSVVYQIDQESKT
                     ISDIPKNTTVEQFCKDSIVDTGVTITVKSKDGKPLENADIIKGGMTVTVSCEGKEAVV
                     YTVVASSDNKLKSAYYEVKDKTIYVPFTEKNPTTAGELKGNVQAAETAEVSVVSGEKT
                     LKDQENIADAMTMRITAEDGKTNDYTIKQKNTYNWALDYAGPQQGNVWFGQKKAASGE
                     WTE"
BASE COUNT         5870 a         3194 c         5144 g         4819 t
ORIGIN      
        1 cgcgtcaatc gtgttgggaa taatgatact gcagactata atatccgtca gcgagtcggc
       61 gataagagcg gtgcctgaaa gttattatga aggttcgctg gcgcttgggg caagtcacga
      121 gagaagcgta tttagggtag ttctgccggc tgctgcttcg ggagttatgg ccggcgtgat
      181 acttggcgtc gggcgtgcta tcggagagac tatggcggtt attatggttg cgggaaacca
      241 ggcggctctg ccgagaggat tgttcagcgg cgtaagaacc atgactgcaa acattgtttt
      301 agaaatggga tatgccgcag atttgcacag agaggccctt atagctaccg cggtggtgct
      361 gtttgtgttt atcctaatca taaacgtctc tttttcagtt ttgaaaagga ggtcggaaaa
      421 taaatgagaa taattttgag accgtcaggc gaggcagcgg cggaacaaag atttgccggg
      481 aggcgcagcg gaatgaataa atcaaagcta cagtcagtga ttttaagaac tcttgtatat
      541 acagcggcgt ttataacatt cggcgttctt atttgtctta ttgcttatat attgataaag
      601 ggaattccaa acttgtcgct agaccttttc agttttgaat acaactctga aaatgtctcg
      661 ctttttccgg ccttgataaa cacagttgtt atgacggctc tgtcattggt tatagcggtg
      721 cctttgggag tgttttctgc tatatatctt gttgagtatg caaagagagg aaacaaaata
      781 gttggcctta tcagactgac tgcggagacc ctttcgggaa taccgtccat agtttatgga
      841 ttgttcggat tccttttgtt tgtcacagca ttttcgtggg gatattccct gcttgcggga
      901 gcgtttactt tggttattat gatactgccc gtcataatga gaaccactga ggaagctctt
      961 aaagctgttc cggatacttt tcgtgaaggc agttttggtt taggcgccgg gaagcttaga
     1021 acagtgttta aaatagttct gccgtcagcg gtgccgggga ttttggccgg agttgtttta
     1081 tctgtcggca gaattgtggg agagactgcg gctttaattt atacagcagg aacagtagct
     1141 cagattccga gcggtttatt cggttcagga agaaccctcg ctgtacatat gtacgctctg
     1201 tggagcgagg ggctgaacac taatcaatcc tacgctacag ccgttgtgct gcttattata
     1261 gtagtgatac tgaacgcggc ttcatcggca ttggctaaga aaatttccag caaataatat
     1321 tgggtttatg gattccagca aaatccaaat ttaagtataa ggataaaaaa ttatgagtgt
     1381 gatatccatt aaagatttaa atcttcatta cggggatttt catgctttaa aaagtgtaaa
     1441 catggaaata cctaaaaatg aaataaccgc atttatcggt ccttcaggct gcggcaaatc
     1501 aacattactt aaaaccctta atagaatgaa tgatttggtt gagggctgca gaatagacgg
     1561 agaggtaaaa cttgacggta taaatatata taaggacatg gatactaatt tgctccgcaa
     1621 acgggtggga atggtttttc aaaagcctaa tccctttccg atgagtattt atgacaatat
     1681 agcgtatggt cctagaacgc atgggataaa atcaaaggct aagcttgacn nnnnnnnnnt
     1741 ccaaaagtcg cttgagcgcg ccgctatatg gaatgaagta aaagatagat taaagaaaag
     1801 cgcgcttggt ctgtcaggag gacagcagca gagattgtgt atagcgaggg cactggctgt
     1861 tgaaccggag gtgttgctta tggacgagcc cacgtcggct ctcgacccga tttcaacttc
     1921 aaaaatagaa gaccttgcgc ttgaattaaa aaagcgttat actatcgtca tagtgaccca
     1981 tagtatgcag caggcaacgc gtatatcaga taagaccgca ttttttcttt tgggtgaagt
     2041 tgttgagttc ggagatactg aacaaatatt ctcaattcct aaggataaga gaacagaaga
     2101 ctatatcaca gggaggtttg gttaagctat gcgtaacaga tttgacagac agcttcattt
     2161 attggacgag cagcttaccc atatgggcga gttgtgcgag actgcgattg caaaagcaac
     2221 gcaggcattg aaggacggaa gcattgaaca ggcaaaagtg atcattgccg aggatgagga
     2281 gattgatcag attgaaaaag atattgaacg tctctgtctg aaacttctcc ttcagcagca
     2341 gcccgtggca aaggatcttc gcaggatttc tgcggctctg aaaatgatca cagatatgga
     2401 acgtatcgga gatcagacat cggatattgc agagatcatt ttgtcgtcaa aaggagttgc
     2461 ggctacagat gagatccatg taatcggtac gatggcatct gaggtgagca agatggtccg
     2521 caccagcgtc acggcatatg ttgagaaaga tttagagctt gcacgcagcg tgatgaaggc
     2581 ggacgatgag atcgatcagt atttcgagga agtgcgggat gagatgatcg agtttataag
     2641 agaagaaaaa ggcgagaacg ggaaacgcat ttttgatctg atcatggtga caaaatatct
     2701 ggaacgtatc ggagatcatg cgacgaatat tgccgaatgg gtagaatttt ccattacggg
     2761 agtgcataag gatcatgcga tcatacatgc ggaataatga acaaaacaag taaaggagaa
     2821 ctggaagaat gatatattgc gtggaagatg acgacaatat cagagaactg gtcatatata
     2881 cattggagac aacggggctt aaagccagag ggtttgcgga tggttcggcc tttacggagg
     2941 ctctggcgtt tgaaacgccg gagcttattt tgcttgatat catgcttccg ggagaagacg
     3001 gtctggagat attaaaaaaa ttaaaatgtt cgtccaagac gaaaagtatt ccggtgatta
     3061 tggtaactgc aaaaggatcg gaatatgata aagtgatcgg tctggattcg ggagcggacg
     3121 attatgtgac aaaaccgttt gggatgatgg agcttgtttc ccgcatcaag gcggttcttc
     3181 gcagaagcaa aggatcgcag gaaagggaaa atccggaaat acaaggagtt catattgatg
     3241 tgaagaaaca tgaagtgacc gtagatggac ggacagtacc tcttacatta aaagaatttg
     3301 aactgcttga aaagctgatg agaaacgaag gaattgtttt gacaagagat cagttgctta
     3361 cagaaatatg gggatatgat tttgacggag aaacaagaac tgttgatgta catatcagga
     3421 cgcttagaca aaagctgggt gaaaagggag aaatcattca gacagtacgc ggagtcgggt
     3481 atcgcatagg aggaagcgca tgagtataaa gaaaaaaatc cagcaaagca tgactgtgat
     3541 tttgacggta actcttgtgc ttgcttatgt gatcctttcg gttatccttt ataatcggaa
     3601 tatgaatctg cttcagacag aagtaaaaca ggaagcggca tatattcgtg aagcgatcaa
     3661 tttgtctgac agcgattata tgaaacagat ggataatgtt gtggaaagca caagggtgac
     3721 gcagatcaaa gcagacggaa cggttctcta tgattcacgc agagatgcag acatccttaa
     3781 aaaccacgga aaccgaaaag aagtcagaga agctctttct aaaggagaag gggcagacgt
     3841 aagaaagtca gaaacggtag ggaaggatat gtattattat gctcttcttc tcaacgacgg
     3901 aacggtgctg cgcgtatcca agtcgattga taatctggca agtaccgcag tcagtctaat
     3961 gccggtgatg ggaattcttg ccgtactgac aatattgttc gctttcgctt tggcaagatg
     4021 gcagacaaac cgattgatca aaccgatcaa tgaacttgat ctggaacgtc cattggacag
     4081 tgatatttat gaggaattaa gtcctttgct ggaggcgatg gaccggcaga acagagaaaa
     4141 agaagcggtt gcgaatatga ggaaagagtt ttccgcaaat gtatctcacg aactgaaaac
     4201 gccgcttacg tcgatttccg gctatgcgga aatcatgaaa aacggattgg tccgtccgga
     4261 agatatttcc ggtttttcgg aacgcatcta caaagaggcg cgcagactga tcaccctgat
     4321 cgaagatatt attaaattat caaagcttga tgaagaatct gtggaattgg aaaaagaaga
     4381 tgtggatctg tatgaactga cgagggagat tgtcagtcgt ctttcgccgc aggcggcaca
     4441 gaaaaatatt cggatcgaac tttcgggaga atctgttcat tatcatggaa tccggcagat
     4501 attagatgaa atggtttata atgtctgcga aaatgcagta aaatataact atgaaaacgg
     4561 aaaagtttcg gtttgggcgg gaaatacgtt gaatggaccg aaaatcagtg tgtcagatac
     4621 aggtatagga attccgaaag aacatcagga acgcatattt gagcggtttt accgagtcga
     4681 taaaagtcat tcaaaagagc gcggaggaac gggactcgga ctttcgatcg tcaagcacgg
     4741 agcgcttctg catggggcga aggtcagtgt ggaaagtgaa gaaggaagag gaacgaagat
     4801 cagtatgcag tttccgcgaa tttgtcagaa ataaatagga acaccgataa aacataaagg
     4861 gtctggtatc aggctctttt ttgttctctt aagaagcttg aaaaatccat cagaaaaagt
     4921 ttaattaaaa gtttgaaaaa ttataagatt gtgctattat acatataaca aattccgaaa
     4981 ggagaaagat atgatcatag aagttgattt taacagtgat gaagcgattt atattcagct
     5041 ttgtaatcag atcatcatgg ggatcgctac atcggtcatt catgaaggag attcacttcc
     5101 gtcggtgcgg cgtcttgcgg atacgatcgg cgtcaacatg cacacggtaa acaaagcata
     5161 tagtgtactg aagcgggaag gctatatcag tcttgacaag cgaagaggag ctgtaatttc
     5221 tcttgatatt gataagttag aggaactgca ggtgatgaaa agacagcttc agatcgttct
     5281 tgcaagaggc tgttgtaaaa atatttcgag gcaggaagtg catgaactgg tagatgagat
     5341 attcgatgat tatgaataat ttgttttagc agatttgaaa gcaaaataac cgacaggagg
     5401 aacttatgca tatttcatat acaaaacagg cggcaaatgc agtcagatat gctgcgaaga
     5461 aagcaaagga gatgaagcat ccttacatag gtacggaaca tctgcttctc ggactgcggg
     5521 aggagttttc gggggttgcg ggacaggtgc ttgcgcaaaa cggcgttgag acagaaaaga
     5581 tcatgcagct tatggatgaa ctgatcgctc cccgggagga gatgcccgca tcgcagaaac
     5641 cgaaagaaag tccaaggctt cgctatatcc tcgcgaacag tgagaaggaa gcgcatcgtc
     5701 tccgtacagc cgaggtcgga acggagcact tgcttttgtc gatgatacgg gatgtggact
     5761 gcgtcgccgc aagaattttg atcacgctga atatcagcct tcaaaaactt ttaaaagata
     5821 ttttaaatgc atcgggagtc gatccgaaag attatcagga tgaacttcag gacgagtcga
     5881 gaggaagcgg ctctgtgatc gagcagtatt gtacagatat gacggctcgg gcagaggagg
     5941 gaaaacaaga tcccgttgta ggcagggaag aggagatgta tcgccttatg caggtgttaa
     6001 gccgcaggac gaaaaacaat ccctgtctga tcggagagcc gggagtagga aaaacggcgg
     6061 ttgtggaagg tctggcgcag cgtatcgcgg caggcgttgt tccggaaaaa atgaaagata
     6121 agcggattta tacgcttgat cttccgggaa tgatcgcagg gtctaaatac cggggtgaat
     6181 ttgaagaacg tatgaaaggt ctgatctcgg aagtagagtc caacggaaat attattttgt
     6241 ttcttgacga gatccacaca atgatcggag cgggaggagc agaaggggcg atcgatgcat
     6301 ccggcatctt aaaaccgtct cttgcaagag gggaacttca gttgatcgga gcgacgacga
     6361 tcacggaata ccggaaatat atcgagaaag atgcggctct ggagcggcgt tttcagccgg
     6421 tatcggtaga agaaccttcg aaagaacagt gtcttgagat attgaaaggg ttgaaaggaa
     6481 gatacgaatc gcatcataag gtgctgatta gagacgaggc gctggaagcg gcagtttcga
     6541 tgtccgagcg gtacattacg gatcgtaacc ttcccgataa agcaatagat gtgcttgacg
     6601 agtcatgttc aaaagtcagt ctgaaaggat ataaagtacc ggaaaacctg acggctttgg
     6661 atcttcgcct gaaagaactg gaaaagcaaa aagaagagag tattaaaaac ggttgttttg
     6721 aagaggcttc gcttttacag aaagagcagg aggaggcaga gaaaaaatcg gagcagctaa
     6781 aaaaacgttt tcagaaaaaa acatcttctt ctcagccgga agtgacagaa gaagatatag
     6841 cggaagttgt ttcctcatgg acaaagatcc cggttcaaaa acttgcggaa agcgatacgg
     6901 atcgtcttaa gaaactggaa agtgtcctcc accagagggt catcggtcag gaggaagctg
     6961 tgaaagcagt tgcccgtgcg gtaaagaggg ggcgtgtagg attgaaagac ccgaaacgcc
     7021 cgatcggttc gtttctgttt ttaggaccta caggcgtcgg aaaaacagag ttgtcgaaag
     7081 cgcttgccga agcgatgttc ggcaatgaag aatcgatgat ccgtgttgat atgtccgaat
     7141 atatggagaa acattccgtc tctaaaatga tcggatctcc gccgggatat gtcggacatg
     7201 aagaaggcgg tcagctcagc gatcaggtgc gtacacatcc ttattcggta cttttgtttg
     7261 atgagatcga aaaggcgcat cccgatgtgt ttaatattct tttgcaagta ttggacgacg
     7321 gacatattac ggactccaaa gggcggaaga tagatttttc caataccgtt atcattatga
     7381 catcaaacgc gggagcaaaa gcgatcatag aacctaagaa gcttggattt gcggcaaaag
     7441 atgatcctgc aggcgattat aagcgtatga aacaaaacgt gatggatgaa gtaaaacaga
     7501 tctttagacc ggagttttta aaccgtattg atgagatcat cgtattccat gctctcgaga
     7561 aaacgcatat gaaaaagatt gttacgctta tgtgccggga ttttacaaaa aggatcgaag
     7621 atcagatgga tatccgcctg actttacggg agtcggcaaa agcgcttatt gcggaaaaag
     7681 gaacggatgc aaaatatggc gcaagacctc ttcgccgcgc tctgcagaca gaattggaag
     7741 acaaacttgc ggaagcgatc ctgaacggag aagtgaagcg gggagactgc attgaggcgg
     7801 gaactgtgaa aaaagagatt cgcttcattc ggaaagaaga tcgtttatag gagtagaaaa
     7861 atcaaatatt ttatattata atggtacaaa ggtcaaagga atgcgaaaga caatgattta
     7921 cacaaaacat atcctgtgat cacattgtga aatacttagg aggaaaatga agatggcagc
     7981 agtgaaggaa ttattgcgcg cggagaatga cggaagtctc agtttcggag actacactct
     8041 ttcggaaaaa acgaagaaag ataattatga atttgaaggt gatatttata aagtaaaaac
     8101 cttttcggaa attacaaaac ttgagaggaa cggaatgttc gtttatgaat ctgttccggg
     8161 aagcgcagtg gaaggtttta aagagacgga ttcagaagtt gcgttttctg tttccgcaaa
     8221 gggagacgtt cagtttactt tggaattaga gccggaatgt gagtatgaag tatttattga
     8281 cggcgagtcc gcaggaaaga tgcgtacgaa tttaagtgga aaactcagtg tcagcgtgga
     8341 actttcagaa ggacaggaag cgacaatgag agttgtaaaa tgttaatata ttaactgaaa
     8401 agtttacaaa tacttaaatt gatttcgggg tggatgctat atccaccccg tttgtctgta
     8461 ggcagatctt atgctgagag gcgtttagaa tctgtctttt aagatggatg tgtcaaatct
     8521 gattgtttaa tgagtgtttg attttgagaa ggagaatgaa tggcaaaagg aaagaaaagt
     8581 gtgtttttct gtcagaattg cgggcatgaa gaggcaaaat ggctcggtca gtgtccggga
     8641 tgcggggagt ggaatacatt tgtagaggaa aagatcggca gcggtgtgac gaaaggcagt
     8701 acacagggag caaaagctgt tcgcgagctt gtcagagaag cgaaagtgat cccgcttaac
     8761 gatgtaacgg ctgaagatga cgtgcggtgc gctacaggca tcaaagaatt agaccgcgtg
     8821 ctcggaggag ggatcgttcc gggatcgctt gtgcttgtcg gcggcgatcc cggtatcgga
     8881 aagtctactt tgctgcttca agtgtgcagg attctggccg ataaaaagaa agttttatac
     8941 atttcgggag aagaatcgca gacgcagatc aaactaaggg caaaccgtat gggaaacttt
     9001 gcggacggat tgtttctttt atgtgagacg aatctggaga tcataagagg aattcttgaa
     9061 aaagagcgtc ccgaacttgt tgtcattgac tctattcaga cgatgtacag cgaagaagtt
     9121 tcatccgctc ccggaagtgt gtcgcaagta agagaatcga cgaatgtatt tatgcagctt
     9181 gcaaaaggat tatgtattcc gattttcatt gtcggacatg tgacgaaaga aggaacggta
     9241 gcaggtccgc gtgtcttgga acatatggtt gatacagtgc tttattttga aggagacaga
     9301 cacgcttctt acagaatttt acgcgctgtg aagaatcgtt tcggatcgac gaatgagatc
     9361 ggcgtgtttg agatgcggca gagcggactt gaggaagtgg aaaatccttc ggaatatatg
     9421 ttgagcggca gaccggaaca gtctgcggga tcggtcgttg catgttctat ggaaggaact
     9481 cggcctattt tgatcgagat acaggcgctt gtatgcagga gtaatttcgg gatgccgagg
     9541 cgtacggctg ccggaacgga ttataacaga gtgaatcttc ttatggcagt gttggaaaaa
     9601 cgtctcggaa tggcgctgtc taattcggat gcgtatgtca atatcgccgg aggaatcaga
     9661 atgaacgagc cggcgatcga tcttggtatt gttatggcgc ttgtttcaag ttacagaaac
     9721 cgcccggttg atgaaaagac gatcgtgttc ggggaagtcg gactaagcgg agaagtccgc
     9781 gcggtcaata tgcccgaaca aagagtcgca gaggcgaaaa agctgggatt cgagacgtgt
     9841 attcttccgc aggtgtgcat gaaaatggta aaagatatca aaggaattcg actcatcggg
     9901 gtaaaaagcg taaacgaggc ggtcgggatg atataagcgc ctgttcatga gtaaaccgct
     9961 gacgttagct gcgataagca cgcagcgcgg ttttaggaat ggcgcgggct gaactgttat
    10021 ggcctgaatt ttttgaatgg aaaaaatatg cggttgtaaa aatagtataa gtagagtata
    10081 atagcagtaa gttaaaagag aaataacctg agatgtgcga taaccttgtt attttgaacc
    10141 tacattagtt taaacgggat tcctatattt ctgtaatatg tcaggcctcc gacagcagtg
    10201 gacgacagaa aagcagatgc ggttgcccac ctagccgtgg ctaggactca aaatacaggg
    10261 aacggcattt tggggagtta caaaagataa aggcggccgt cctgtacgac cgctttttgc
    10321 aatggcgata agtcgaagtt tgggtttgcc ccggagcttg gcagccgttt acaatcccgg
    10381 tacgagcctt tggtgattcc ggtgattcaa atgaaattaa atggaattat gatgtgaata
    10441 tgaaaaaaaa gtgggaaaag ataaaaagtc agatatttaa agttattata acgataaatt
    10501 ctcatcacac aggcgcttat gcagcacagg cggcttattt ttttgtgctg tctttgatcc
    10561 ccatattttt gcttctcttg acgatggttc gctttacgcc gataacgatg agcgaagtta
    10621 tgacagcggt tttgaaagta tttccggaaa gtgttgcgcc catgatacgt tcgatcgtat
    10681 ctcaagtata ttttcagtca ggaacgatcg ttccggttac gattgttgta gcgctttggt
    10741 cagcggggaa aggcgttctg tctgtcacat cgggattaaa cagcatctgt gagaatatgg
    10801 agacgcgtaa ttatttttat ctgaggataa gagcatcgtt ttatacagtt atattcctat
    10861 tggctatcgt aagctccctt gtattatctg tattcggaaa caggatcagc gtgatgatat
    10921 atgagcacat tccgttttta agcaaagttg tcgaatttat tattcggatc aggacgtttg
    10981 ttacatttgt cgtgttgact gttttttggg atcttgtgta ccgttttctt ccgaacagga
    11041 gaaatatggc aaagacaact cttcgcaaac agcttccggg agcggtgttt acggcatgcg
    11101 gatggctgct tttgtcgttc tttttttcgg tataccttga tgtgtttaaa ggctttacaa
    11161 gtatgtacgg aagtttgacg acgattattt tgatcatgct ttggctgtat ggatgtatgt
    11221 acattatatt gctgggcgga gagatcaatg cgcttctgga aaaatttttg cagaaacggg
    11281 caggcaattt gaaaaagaag aaagagaaag aagtataatc cctcgggcaa aagcatagtc
    11341 ttttgttacg ggggtgattt attattgatc tatgttgtgg aaaaaggaga tacgttgtcc
    11401 ggcattgcgg taaaatttgg cgtgcccgtt tggaagatcg tttatgataa tcagctaaaa
    11461 gacaaaaatc agatcgtgcc cgggcaggcg cttcttttgt tggaagttga tgaggggcag
    11521 gcagaggaaa gtacacagcc tgaattggca aaagggaaaa cgatcggcgg atatgcttat
    11581 ccttttatag ataaagaaat tttagaacag tcgtttcctg cgttaaaaga acttcttgta
    11641 ttttcttatg gttttacatt tgaagggaat ctggttccgc ctttgcaaga cgatctttgg
    11701 atgatagaga ccgcatggcg tcacggagca aaaccgatca tggttcttac gccgttttcg
    11761 gaaggagctt ttaataatca gcttgtaaaa gtgcttacgg aaaatgtcga ggtacaggaa
    11821 aggcttttgg aacagttgac tgctgaagta agaaggcggg gctttgcggg agtcaatatt
    11881 gattttgaat atgttttgcc tgaaaataaa gttcagtatg ctgcgtttat cgggaaaatg
    11941 agagaacttt taagaccgga aggatatacg gtatctgtgg cagttccgcc gaaggtgtca
    12001 aacgaacaga gagggcttct tgtagaaggg gcggattaca gactgatcgg ggagaacgcg
    12061 gattacgttt ttttaatgac ttacgagtgg gggtatacgt acggacctcc gatggctgtc
    12121 gcaccgcttg ataaagtacg tcaagttgtc gagtatgctc ttagccagat cccggcggaa
    12181 aaattgattt tggggatacc aaactatgga tatgactggc agcttcctta tgagagagga
    12241 gttacaaggg ctcagacgat cggaaatgtg caggcagtgg atcttgctgt tgaaaacggc
    12301 gcgttgatcc ggtatgcgca gactgcccaa agtccgtggt ttacatataa gaaaagcggc
    12361 atggatcatg ttgtctggtt tgaagacgtc agaagtattc aggcaaaatg gaaactcgtt
    12421 caagagttag atctggcagg agcctggtat tggaatttga tgaggaaatt tcggggcaac
    12481 tggctgatgt tagagtcccc ggatgcagga agaaacactt gacatgaagc agggggaaag
    12541 gagtatgata tcaagagaag ataatgagat aaatgaaaag gagacgatgt gatatgaacg
    12601 gaaattttgt ttactgcaat cctacgaagc tttatttcgg agaagattcc cttgattttt
    12661 tgaatgagga gcttcctaaa tacgggaaaa atgttgtact tgtttacggt gcgggttcta
    12721 ttaaagaaaa cggaatctat gataaagtaa taaatatatt aaaaaagaat gaaaaaaata
    12781 tcgctgagat tgccggggtt atgccgaatc cgactttggc aaaattatat gaaggaatta
    12841 agattgccag aaatcataat gcagatctgc tgcttgcggt aggcggcgga tcggtatgtg
    12901 attattctaa ggcggtgtct gtatcagtga actgcacgga agatccgtgg gagaaatatt
    12961 ttgtgcggtt tgatgagcct gattgtgaga tcgttccggt cggctgtgtt ctgactatgg
    13021 ttggaacagg atcggagatg aatgcgggat cggttattac gaacgaaaag acgaagcaga
    13081 agatcgggca tgtgtttgaa gatgagaaga ttatgcccag attttcgatt ttgaatccac
    13141 gttttacaat gacactgcca cattatcaga tgactgccgg aatttatgat atttttaatc
    13201 atatttgcga acaatatttt tcagatacag atgataatac gagcgattat atcagtgaag
    13261 gcttgatgcg ttctctgatt cattcaagta agatcgcaaa taaagacccg caggattatg
    13321 aagcgagaag caatattatg tggacggcaa catgggcgct taacacgctt gtcgcatgcg
    13381 gaaaatcgac agactggatg gttcatatgc tcggacaggc agtaggcgga tatacaaacg
    13441 cgacgcatgg gatgacattg gcggcagttt cacttccgta ttatcgtcat attatgccgt
    13501 atggtcttga aaaattcaaa cggtttgcgg taaatgtatg gggcgtggat gcttcggaaa
    13561 aagaagatcg ggaaattgcg gaagaaggtc tggcagctat ggaaagctgg atgaaagagc
    13621 tggggcttgt tatgaatctg tcagaacttg gggtgacaga agagatgata gaagggattg
    13681 cggatgtgac gatcgtattc gataccgggt acaagaaact ggatcgaaca gaaattatcg
    13741 atattttcag ggaaagtctc atatagtaac agttcagtct gcgtcattcg taaaaccgca
    13801 ctgcatgctt atggcggctg atgccactgt tttactcata aacgggcggc catctgtatg
    13861 gctgaacttg ttaccttata taacgagaag ttgaacagtc tgttccgatt atgctatact
    13921 tgacagagag ctgccgaaag gttcggcaaa gaatcatctg tactatttta taaaagtcag
    13981 gcagaagatc gggaggttgt gatgacgggc ggtattgcga aagtagtcat attcagtttt
    14041 acagaaagag gaacgaagca gaatcgatac atatatagga aattaaagat gcaggggtat
    14101 atctgtgaag gatatgcccc tgcaaagtat gttatgggag aagaaggcgg ggtgaagcaa
    14161 cttccgaaaa aaaaggaaga tttgattcgg gaacgctggg gaaagtgggc atttttattt
    14221 atcggtgcgg cagggatcgc agttcggtat attgcgcctt atgtgaaaga taaatttacg
    14281 gattcggctg tcgtcgtggc ggatgaagcc gggcagtatg tgatcccttt gttgtccggt
    14341 catgtgggag gagcggcaga gctcgcggat cagatagcaa atctgctagg cgctgtcagt
    14401 gtgcatacga ctgctacaga tgtgagaaaa aagtttgcgg ctgatgtatt tgcaaaaaaa
    14461 aatgcattgt atattacgga cagagaggcg gcaaaaagaa tttccgcagc ggtgcttgac
    14521 ggcgaattga tcggaattta tgcgcaaggc gatcatgcag aaaatgccgt aaaaaattca
    14581 aaagagatgt tttcggatga agtttgcata tgtaatacga tagaagaagt aatgaagtat
    14641 cggtatcggg tcattcttgc agattcgttt cctgcgatag atgaaaatac acttctttta
    14701 aagccgaaaa atattattgt cggacttgga tgcaggaaag gaatttcaga agaactttta
    14761 gaaaaaggtc ttttagaaat tttgaaaaag aatcatctgg atatgaatca gattgaagcg
    14821 cttgtaagta ttgatctaaa gaaagaagaa cctgccgttg tttcgctggc ggaaaagtat
    14881 aaagttccgt ttttgacata ttcggcggaa atgctcaacg aggtggaaga agtctcatct
    14941 gcatcttctt ttgtcaaaaa aataacagga atagataatg tgtgcgagcg tgctgcggtg
    15001 acatattgta agaaacactg cgagtatttt gaactgattg ccgggaaaag catcgagaca
    15061 gcgatgactg cggcaatcgc aagacgaaat atataaagac agaagagtga aaggaaaaag
    15121 gcacggagga agaaacaaat ggtatttgaa ttacaatttt tagattggct gcagacgctg
    15181 cagacgccga ttttaaacga aattatgaaa gcggtcacac atcttggaga cgcgggcgct
    15241 ctgtggatta tactggcggc tgttctgctg gcatttccga agacgagaaa aagcggggcg
    15301 atcgttgcgg ctgcgcttat tgtcgatctt gtgttatgca acgggatctt gaaaaatata
    15361 tttgcccgca cgcgcccgtt tgatgtaaaa gaagggattt cgctgctgat cacggcgccg
    15421 aaagattttt cttttccttc gggacatacg gcggcatcgt ttacagcggt tgcggcgctg
    15481 tattttgcag gagagaagaa actttggaaa ataagctgtg ctgtggctgt tttgattgcg
    15541 ttttccagaa tgtatctgta cgttcattat ccgacggata ttttgggcgg gatcatcgtc
    15601 ggaatcgtat caggacttgt cggggcgtat ataataaaac gggtacagaa aagaggcgta
    15661 gttccgaatt agataaaaag acttgatgaa tatgcaaaaa tgaacgagaa gaaataacag
    15721 ggaattgtta tgataattta aagctgttca tttgcaggga gacagacatt atgaaaaatg
    15781 aaaatcagca tttctcagca gacagacgga aattcggcag acatttttgg atcggcgcgg
    15841 cagtggccgg agcgctttcg gcggcggtta tatttacggc ggcgagtgcg gtttaccgaa
    15901 agaatcgttt cgatctgaaa atcggagaag aaaaagttgg atcaaaggaa tatcagcttt
    15961 gtatggatct tgtaaaatat gatgtgaaga tggagatcca aaagcggtat gacgctttgt
    16021 acggtgaaga tttttgggag caaagctatg aaggacaata tggatatgag attttagcgg
    16081 atcacacggt ggaaaagatc aagtatattc atgcggtgta tgatctggcg gaagaatacg
    16141 gcgatgtatc cgacggaagc tttgcggcga ttgaaaaacg gtggaagcag gaaaatgcaa
    16201 aaaggcagaa aaaggtagaa gacggagaag tcatctacgg tctgaaagaa tacacgtttg
    16261 acctttatat gcagtatgag atcagtatgt ataaagaaat ttactgtaat gatactgaca
    16321 gaaaaggaat ggatctgacc gaagaagaag tagccgaata ttacagtcag ggagaatggg
    16381 tttttcaaga cgacggggaa aaggctgatt tggaaactgc gcgcattgcg gttgagagag
    16441 aactgcgtga gaaaaaatat gatgccatga tcgctcagat gacagaagat ttagaagtaa
    16501 gcggtgattt ggaagcagtt gatcgtttta ctttggatca tttaaaacga taacagtcaa
    16561 ggcggcgccg gcatatgacc gggaatataa agaggcgctt tggagattgt atcaggtggg
    16621 aaggagaaaa gtatgaagaa gaaatgggtg agcggaatgc tcgcgctctt gcttgtcgga
    16681 acgactgtcg gatcgatgat gccgacagag gcggtgcagg cagaagaaaa cagtcaggga
    16741 aagacgtatt atgtcgattc tgaaaacgga aaagatacaa acgacggact ttctgaagga
    16801 aaagcatttc agacactgaa caaagtaaat gatcttacgc tcggagcggg agaccgagtt
    16861 cttttgaaaa acggatcggt gtttgaggat caggcgcttc atattaaagg aagcggaagt
    16921 gaaaacgctc ctattaaaat ttctacatat ggggatgaga aagacggacg tcctcagatc
    16981 aatacaaacg gtcatggaca atgggaatta aattacgggc acaaacttga caatcagaac
    17041 cataagtggc acggaacagt atcttcgtct attttgctga aagatgtaga atatatcgaa
    17101 atcgaaggac tggagattac aaatgaccgg gattccgcaa cagatgcaga gaaagataaa
    17161 aattataaat acaatgacgc ggaatgcatg gacaggacag gcgttgccgg agttgcaaaa
    17221 aacaaaggaa cggttgacca tatcgtattg aacgatctgt atatccatga tgtgacaggc
    17281 aatgtttaca ataaacatat gacaaacggc ggtatttatt ttattgttga aaagccggag
    17341 aacgagagcg cgacgggaat tgccagatat aatgatgtga cgatacaaaa ctgctatctt
    17401 gatacggtaa accgttgggg aattgcggtc ggatatacat atgaatgggc aaaattcaac
    17461 ggcggcgctt taagtgatga gacgatgaaa acatacgcgt cctcagacgt tgtgatccag
    17521 aacaattatt tgaataatgt cggcggagac gcgatcacga caatgtatat cgacagaccg
    17581 gtcatccagt ataatgtatc ggaaaatgcg gcggctcaga ttaatacgac agattataca
    17641 gatccgcagc cacagcttga cgcaaacgga aatccaaacg gaaagacgca tacaggagga
    17701 agagtcgcgg caggtatctg gccgtggaaa tgtaaaaatg cggtgttcca atataatgaa
    17761 tgttttaaga cgttaaatgc ctcaaaagga aacggagacg gacagccgtg ggatgcagat
    17821 tatggagacg gtacaaatta tcaatataat tacagccacg gaaatacggc aagtacgatt
    17881 atgttctgcg gaggcgaatc gatcaataat acgttccgtt ataacatcag cgtacatgaa
    17941 gatatgggac cgcttgatcc ggcaggaaac gcaggaaata cacaggtgta caataacaca
    18001 tttgtgatca aagaaggaat ccggtcgatc tggtacagag attccggccc ggttacgatg
    18061 gaaaacaata ttttctattt tgacggagag cagccggcgc agattacaaa ctggaatccg
    18121 cgtaataata aagtttattc caacaatctg ttttataatg taagttctta tccggatgat
    18181 aaggctgctg tgaaagtgga aaaaggaact ccggttcttg cagacgcagc gtcaggtccg
    18241 gtaaaagctg cagaagacaa acaggcaaga cggcatgaag atccgacgga aataaccgtt
    18301 tttgatggat ttaaactggc ggaaaattcg ccggcgatca ataaaggaaa agtagtgatt
    18361 gaccgtaacg gttattcaat cgatcacgat ttcttcggac atgcagttac ggcgacgccg
    18421 gaaatcggag ctgcggaaag cgatgtgatc ggagatctcg ttcttcgttc cgtagtttat
    18481 caaatcgatc aggaatcaaa gacgatcagc gatataccga aaaatacaac agtagaacag
    18541 ttttgcaaag attcgattgt agatacggga gtgacgatca ctgttaagtc aaaagacggc
    18601 aagccgttgg aaaatgcaga tatcattaaa ggcggcatga cagtaaccgt ttcctgtgaa
    18661 ggaaaagagg ctgttgtata tacggtagtt gcaagttcgg ataataaact gaagtctgca
    18721 tattatgaag tgaaagacaa aacgatctat gtgccgttta cagagaagaa tccgacaacg
    18781 gcaggcgagc tgaaaggaaa tgttcaagcg gctgaaacag cggaagtatc tgtagtaagc
    18841 ggtgaaaaaa cgttgaaaga tcaggaaaat atcgcagatg ccatgacaat gaggatcacg
    18901 gcggaagacg gtaagacgaa cgattacacg atcaagcaga aaaatacata taactgggcg
    18961 ttggattatg caggaccgca gcagggaaat gtatggtttg gtcagaagaa agcggcatca
    19021 ggagagtgga cggagat
//
LOCUS       DBMDXF010000009        19029 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_894_length_19029_cov_2.213705, whole genome shotgun sequence.
ACCESSION   DBMDXF010000009 DBMDXF010000000
VERSION     DBMDXF010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 19029)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19029)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19029
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_894_length_19029_cov_2.213705"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..66)
                     /locus_tag="ACZPD4_00790"
     CDS             complement(<1..66)
                     /locus_tag="ACZPD4_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1377648.1"
                     /translation="MKIIINSSSMVPIYEQIMDQIK"
     gene            complement(287..796)
                     /gene="ilvN"
                     /locus_tag="ACZPD4_00795"
     CDS             complement(287..796)
                     /gene="ilvN"
                     /locus_tag="ACZPD4_00795"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1377649.1"
                     /translation="MENKMKKRWISLYVENQVGVLSKITGLFSGKTYNLDSLTVGTTE
                     DPTVSRMTIATVSDDETFEQIKKQLNRMVEVIKVIDFTNIFVRMKEILYVKVFKCTPE
                     DKVEVFQIAETFKAKVIDYGKDSVLVEFVQTATKNDAVVKLMKEEFKTIEVVRGGSVG
                     IESISMMER"
     gene            complement(813..2531)
                     /gene="ilvB"
                     /locus_tag="ACZPD4_00800"
     CDS             complement(813..2531)
                     /gene="ilvB"
                     /locus_tag="ACZPD4_00800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR1377650.1"
                     /translation="MRQITGNKLLVRALKEEGVDTIFGYPGACTIDISDELYRQDEIK
                     VILPRHEQALVHAADAYARTTGKVGVCLVTSGPGATNLVTGLATANYDSVPLVCFTGQ
                     VARHLIGNDAFQEVDIVGITRSITKYGVTVSKREDLGRIIKEAFYIARTGRPGPVLID
                     LPKDVMSELGSPEYPETVNLRGYKPNTSVHMGQLKRALKMLNKAKKPLFLAGGGVNIA
                     RANKEFTEVVNITNVPVVTTIMGRGAVPTDHPLFIGNLGMHGAYAANMAVSECDLLFS
                     IGTRFNDRITGKLHEFAPNAQIVHIDIDTASISRNIHVHVPIVADAKEAVAKMREYVE
                     PCETKEWLERIAQWNAEHPLTMKVHGSIGPRDIIEEMNRQFDDAIITTDVGQHQMFVT
                     QYAEITEKKQLVTSGGLGTMGYGFPAAIGAKIGNPDRTVIAISGDGGMQMNIQEFATA
                     VLEELPLILCVFNNEYLGMVRQWQKLFYGKRYGMTNLRSGALFRRTNGEEMPEYTPDF
                     VRLAESYGAKGIRVTKKEEIKEAFEEAGKNTTVPTLIEFIIDPEEMVYPMIKPGGTLE
                     EMIVEC"
     gene            complement(2794..3615)
                     /locus_tag="ACZPD4_00805"
     CDS             complement(2794..3615)
                     /locus_tag="ACZPD4_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR1377651.1"
                     /translation="MIDTIVEMFSYPFMVRAFAVGSLVALCAALLGVSLVLKQYSMIG
                     DGLSHVGFGALAVATAFQAAPLSVAIPVVVLAAVLILRIKGNGKIKGDAAIALISTTS
                     LALGVMVISMTTGMNTDVYNYMFGSILAMSAEDVRLSVILAGIVLVLYLLFYNKIFAV
                     TFDETFAQATGVKANLYNTLIAVLTAVTIVLGMRMMGALLISSLIIFPALTSMRVCRT
                     FRSVIINSAVISVLCLVIGIAISYVWATPAGASVVIVNAAALAVYSIVGMIRCRK"
     gene            complement(3608..4318)
                     /locus_tag="ACZPD4_00810"
     CDS             complement(3608..4318)
                     /locus_tag="ACZPD4_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR1377652.1"
                     /translation="MALITCENVSIGYEGQTVVKELNFSIERGDYLCIVGENGSGKST
                     LVKSLLGLKGADRGKIIYGDGLKKNEIGYLPQKNEEQKGFPASVYEVVLSGRLNGRGW
                     KPFYRKEDKEAAAEKMEMLGITDLARQCFRDLSGGQQQRALLARALCATKSLLLLDEP
                     VTGLDPIVTGEFYDLIRKINQESNIAVVMVSHDIETAVENASHILHLQETVRFFGTAE
                     DYRKSRIGKKFLGGEEHD"
     gene            complement(4318..5349)
                     /locus_tag="ACZPD4_00815"
     CDS             complement(4318..5349)
                     /locus_tag="ACZPD4_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR1377653.1"
                     /translation="MKDVKKKIWRRRGPAVLVLCLAVIVQGCAGYPADKDGGHREQNK
                     TNENGRLSVVTTIFPPYDFVREIAGDCVDLKMLLKPGEESHSYEPTPQDMIAIENSDV
                     FIYVGGENDVWVEDILSSMPDKDRVSIRLLDCVETEEEEHVEGMKEPAGDSHHEMHGE
                     EEHEGSVHEIDEHVWTSPVNASVIVEKIKEVLIQKDSENKAAYEENADRYQTELAQLD
                     KRFREVVRQSKRNLLIFGDRFPFLYFAKEYGLEYYAAFPGCAGDTEPSAATMVFLIEK
                     AKDENVPAVLKMELSNADIANAVAEASGTEVRIFYSCHNLSAEDFEKGETYLTMMQKN
                     ADTLKEVLN"
     gene            complement(5336..5824)
                     /locus_tag="ACZPD4_00820"
     CDS             complement(5336..5824)
                     /locus_tag="ACZPD4_00820"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1377654.1"
                     /translation="MRAIVCADKNWGIGYKNRLLVSIPSDMKFFRETTTGKVIVMGRK
                     TLESFPNGMPLKNRINIVLTRDRNYEAKGAVIVHDEEELMNELGKYDTEQIYIIGGES
                     VYKMMLSYCDKIYVTKVDRAFQADTYFPNLDEMSEWKMTQESEEQTCFDLEFCFTTYE
                     RC"
     gene            complement(5848..6696)
                     /gene="thyA"
                     /locus_tag="ACZPD4_00825"
     CDS             complement(5848..6696)
                     /gene="thyA"
                     /locus_tag="ACZPD4_00825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR1377655.1"
                     /translation="MSYADKVFIDMCRDIIDNGTSTEGEKVRPVWEDGTSAYTIKRFG
                     VVNRYDLSKEFPALTLRRTAIKSCVDELLWIWQKKSNNVNELKSHIWDSWADETGSIG
                     KAYGYQMGVKHQYKEGRMDQTDRVIFDLKNNPYSRRIMTNIYVHNDLHEMHLYPCAYS
                     MTFNVTKEKDSEKLTLNAILNQRSQDVLAANNWNVCQYAVLVHMLAQVCGMKAGEFVH
                     VIADAHIYDRHVPMIEELISREPLPAPTFWLNPEITDFYDFTPDDVRLDRYETHPQIK
                     NIPIAV"
     gene            complement(6758..7192)
                     /locus_tag="ACZPD4_00830"
     CDS             complement(6758..7192)
                     /locus_tag="ACZPD4_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1377656.1"
                     /translation="MENRSLPIGFIIKQVNNVYEKDLNERLKKIGITSSQCAVLDYLF
                     HTSKDEVSQRDVEKNLNLKNPTVTGILKRLDEKGYIFCVPNANDRRKKIIYLTEKAYD
                     IRRKMEADRRKLDRELTRGMTKRETEALRKNLDKILNNIADP"
     gene            complement(7267..8544)
                     /locus_tag="ACZPD4_00835"
     CDS             complement(7267..8544)
                     /locus_tag="ACZPD4_00835"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1377657.1"
                     /translation="MRKIQVGLLGLGTVGMGVYKLLRKRAETMDKTIGAQLEIKKILV
                     HNKNKKREGVDETLLTDDWKEIIEDDEIQIVIEVIGGIEPAKTMILEALKAGKNVVSA
                     NKDLIAEEGKEIFDTAQAAGKDFLFEAAVAGGIPIIRPLKQCLAGNDLTDILGIVNGT
                     TNYILTKMFEDGMEFSDALKKAQELGYAEADPTADVEGLDAGRKMAIMASIAFHSRVV
                     FADVYTEGITKITAADIAYAKEFDSVIKLLGVARNTPEGIEVGVYPVMIARDHPLASV
                     RDSFNAVFIHGDAVDDAMFYGRGAGEMPTASAVVGDVIDEARNIEYGCSGRISCTCYQ
                     QLAVKDFGDVKNKFFLRMQVENCPGVLAQIAKVFGEHKVSIARVVQKNAKTDRAELVI
                     VTEKVKEKHMGSALCELKEMKSIFEVSSMIREY"
     gene            complement(8721..8793)
                     /locus_tag="ACZPD4_00840"
     tRNA            complement(8721..8793)
                     /locus_tag="ACZPD4_00840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8758..8760),aa:Gly,seq:gcc)
     gene            9042..9320
                     /locus_tag="ACZPD4_00845"
     CDS             9042..9320
                     /locus_tag="ACZPD4_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1377658.1"
                     /translation="MKFIYPAVFRKNESGGYDAYFPDLECCEASGDTLDDAIDNANEA
                     ARNWIMVEFEEENPVFPYISDINDIETEAGDIVRNISVNIRFYEGWDE"
     gene            complement(9449..11242)
                     /gene="aspS"
                     /locus_tag="ACZPD4_00850"
     CDS             complement(9449..11242)
                     /gene="aspS"
                     /locus_tag="ACZPD4_00850"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1377659.1"
                     /translation="MAESMQGLKRTHRCGELSAANAGEKVTIMGWVQKNRNKGGLVFV
                     DVRDRSGIIQVVCEEGKTDAALIEKAAGLRSEYVVAIVGEVAMRSGAVNDKIATGEIE
                     VIPSELRILSEALTPPFPIEENSKTKEEVRLKYRYLDLRRADLQRNLMMKSQVMTLTR
                     QFFSEEGFLEVETPVLGKSTPEGARDYLVPSRVHPGSFYGLPQSPQLYKQLLMCSGFD
                     RYIQIARCFRDEDLRADRQPEFTQIDMELSFVDVDEVIDVNERYLARVFKEVLGVDVP
                     LPIQRMTWQEAMDRFGSDKPDLRFGMELTDVTDVVKNCGFSVFTGAVENGGSVRGINA
                     KGQGGMPRKKIDKLTAFVKDYGAKGLAYIAIQEDGSIKSSFAKFMTEEEMDALIKAMG
                     AEAGDLLLFAADKNKVVWDSLGALRVELAKQLELLDKNEFRFVWITEFPLLEWSEEQN
                     RYVAMHHPFTMPMEEDLPLIDSDPGKVRAKAYDIVLNGNEIGGGSVRIFQDDIQEKMF
                     EVLGFTKEQAYSQFGFLLDAFKYGVPPHAGLAYGLDRLVMLMAKQDSIRDVIAFPKVK
                     DASCLMTEAPSVVDVKQLNELGLETTAETEK"
     gene            complement(11235..12485)
                     /gene="hisS"
                     /locus_tag="ACZPD4_00855"
     CDS             complement(11235..12485)
                     /gene="hisS"
                     /locus_tag="ACZPD4_00855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1377660.1"
                     /translation="MALKKKPVTGMKDMMPAEMEVRDYVIGLIKETYKTFGFSSMETP
                     CVEHIENLCSKQGGDNEKLIFKILKRGEKLKLDTAKEEADLVDGGLRYDLTVPLSRYY
                     ANHANELPSPFKALQMGNVWRADRPQRGRFRQFMQCDIDILGEPSNLAEIELILATTA
                     LLGKLDFKNFTIRINDRRFLKAMAAYSGFKEEDYDSVFITLDKMDKIGLDGVAAELKG
                     NGYAEESVEKYLALFEEITNDVEGVRLCKEKLQGYLAPEAADSLEMIITSVESQKEAE
                     FKMSFDPTLVRGMSYYTGTIFEISMDEFGGSVGGGGRYDKMIGKFTGQDTPAVGFSIG
                     FERIVMLLLERGYEVPTSKPKKAFLIEKKLPQEKLLEVLEEARKEREAGRQVMIVNMK
                     KNKKFQKEQLAEQGYTDITEVYNG"
     gene            complement(12476..13954)
                     /gene="hemZ"
                     /locus_tag="ACZPD4_00860"
     CDS             complement(12476..13954)
                     /gene="hemZ"
                     /locus_tag="ACZPD4_00860"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR1377661.1"
                     /translation="MIKVSSKKNKYTYNVYHLVKAFFPNEEIIQNVDEKQESLVALNL
                     PGGSCFSIALEEVEAAAGDLSEMETEEAKEQAIKKELTRLLYRYLEQQTRTTLAWGTL
                     TGVRPTKLAMTKLEQYESMTEAEAVRRTVGAFRDEFFVSEEKAQLACEIAVREKKLLS
                     KLDYKDGFSLYVGIPFCPSVCSYCSFSSSPIAEWKDKVESYLFALLKEIRAIGKMSEG
                     HRPDSVYIGGGTPTTLEAEQMDRLLKTITENFDLSNVLEFTVEAGRPDSITEEKLAVI
                     RKYPVTRISINPQTMQQKTLDLVGRNHTVAQTKDAFYLARKLGFDNINMDLIAGLPGE
                     DASDMEDTLRQVEEMHPDSLTIHALAIKRAARYGQEGRKQDLHSEISQMIMSAADCAK
                     RIGLQPYYLYRQKNIAGNFENVGYAELDKAGIYNILIMEEKQTILAAGAGASTKILLK
                     DPIRTDSGKEINLVRCENVKNIMEYIDRVDEMIERKGEWLWR"
     gene            complement(14053..14676)
                     /locus_tag="ACZPD4_00865"
     CDS             complement(14053..14676)
                     /locus_tag="ACZPD4_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1377662.1"
                     /translation="MRIEKFVFGPVGTNCYIVINEMTSECFAVDMADCPQEFVKHIKD
                     AGLCVKALLLTHGHFDHIMGAERFAEIFGCPVYAYEGEENILCDPVLNASATMLGQSY
                     VFTKAEYVKADTQIETAGFQVEIIPTPGHTEGGCCYYIGEEHVLFSGDTLFHNSIGRT
                     DLPTGSGSELVRSVREKLLVLPEDTRVYPGHMEETTISHEKKYNPFI"
     gene            complement(14788..17091)
                     /locus_tag="ACZPD4_00870"
     CDS             complement(14788..17091)
                     /locus_tag="ACZPD4_00870"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1377663.1"
                     /translation="MAGTAEYEELNKNLEIVDGHAVKAPEDYQDPEQLYHVLIERVRK
                     YHPSADITMIEKAYRIGKEAHRDQVRKSGEPYIIHPLWVGIILADLEMDKETIVAGML
                     HDAVEDTDMTLDDVAGEFGEEVALLVDGVTKLGQLNYSQDKLEVQAENLRKMFLAMAK
                     DIRVIIIKLADRLHNMRTLEFMTPTKQQEKARETMDIYAPIAQRLGISKIKTELDDLS
                     LKYWKPEVYYNLVRDLNRRKTEREEFVQQIVAEVSKHMKNAHIEAKVYGRVKHFFSIY
                     KKMVNQNKTLDQVYDLFAVRIIVDSVKDCYAALGVIHEMYTPIPGRFKDYIAMPKPNM
                     YQSLHTTLMGPSGQPFEIQIRTEEMHKTAEYGIAAHWKYKEGGDSAKSMESQEAKLNW
                     LRQILEWQRDMSDNREFLSLLKGDLDLFAEDVYCFTPNGDVKNLPNGSTPVDFAYAIH
                     SAVGNKMVGARVNGKLVNIDYKIQNGDRIEILTSQNSKGPSRDWLNIVKSTQAKNKIN
                     QWFKKEFKEDNIIRGKELIVSYCRGKSINLQNILKPKYQQVVQKKYGFRDWDAVLAAI
                     GHGGLKEGQVVNRLFEEYEKEHKKEITDETILERISEANSQKVHIAKSKSGIVVKGIN
                     DMAVRFSKCCNPVPGDEIVGFVTRGRGMSIHRTDCINMLHLSDVERARLIDAEWEDTS
                     ETEGGGKYLAELKMYANDRQGFLMDISKVFTEAKIDVKSMNVRTSKKGTATIDMGFIV
                     NGREELHAIIEKLRQLQGVLDIERTTG"
     gene            complement(17152..18870)
                     /gene="recJ"
                     /locus_tag="ACZPD4_00875"
     CDS             complement(17152..18870)
                     /gene="recJ"
                     /locus_tag="ACZPD4_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1377664.1"
                     /translation="MERWYVMMKKADFSSIGAKYNISPVIARLIRNRDIIEDEEINLY
                     LNGTIADLYDGMLMKDMDKAVEILSEKIREEKPVRVIGDYDIDGVNAAYILKEGLEGL
                     GADVDTDIPDRIKDGYGLNKMLIDRALDDGIDTIITCDNGIAAAEEITYGRKNGLTVI
                     VTDHHQVPFVEINKEREYLLPQADAVVDPRRPDCEYPFKNLCGAAVAYKLVEALYNVM
                     LRDPEDVDYLMENVAIATVGDVMALKGENRIFVKQGLEMLKRTKNEGLKALIECTGIN
                     PEYLNTYDLGFVLGPCINASGRLDTAKRALELLSTRTRRDAVMLAEDLKALNDSRKEM
                     TERGIKEAAEMIETTSLKEDKVFVIYLPDCHESIAGIIAGKIRDRYYRPTIILTKAED
                     GVKGSGRSTEEYDMFTELCKCQALFTKFGGHRQAAGVSLKEENVQRFRETINSLCTLT
                     EEDLQMKVYIDMQVPFPYLTEDLIEQFQVMEPFGKGNKRPLFAEKDLKVIQPKIVGKN
                     ENVLQCILEDKQGNRMKAVYFGDVKTCLKEMLAGKVTAVTYFPSINEYMGRRSIQLTI
                     VNYQKK"
BASE COUNT         4734 a         5153 c         3152 g         5990 t
ORIGIN      
        1 tttgatctga tccataattt gttcataaat cggtaccatc gacgaactgt tgattataat
       61 cttcatatac ttcctccttt cgtcaaacag tatataacag tatataactg tttgtcaact
      121 gttatatact gtttatttga aagttcatca taacttttcc aaaatgcaaa tccattttct
      181 tacattaacc cagaaatatc agaaggcaca tttccgttac aaaaagcggg atcgactgtt
      241 ttttcaacaa tcgatcccgc tatactaacc gtcccggcgc ttcggactat cgttccatca
      301 tactgataga ctctattcct acgcttcctc cccggacaac ttctattgtt ttaaattctt
      361 ccttcattaa ttttacgacc gcgtcgtttt tcgtagcagt ctgaacaaac tccacaagca
      421 cactgtcttt tccgtaatcg atcactttcg ccttaaacgt ttccgcaatc tgaaacactt
      481 caactttatc ctcaggtgtg catttaaaca ctttcacata taaaatttct ttcatacgca
      541 caaagatatt tgtaaaatca atcactttga tcacttctac cattcggttc agctgttttt
      601 tgatctgctc aaaagtttca tcatcgctca ctgtagcgat cgtcattctt gatactgtcg
      661 ggtcttccgt tgttcctact gtcagactgt ccagattata tgtctttccg gaaaaaagtc
      721 ctgtaatttt tgaaagtacg ccgacttgat tttcaacata aagggaaatc catctttttt
      781 tcattttatt ttccatattc acgcgctccc ttctaacatt ccacaatcat ttcttccagt
      841 gttccgcccg gttttatcat cggataaacc atctcttccg gatcaatgat aaactcgatc
      901 aaagttggca cagttgtatt ttttccggct tcttcaaaag cctcctttat ctcttctttc
      961 tttgtcacgc ggattccctt tgcgccgtaa ctttctgcca gacgcacaaa atccggtgta
     1021 tattccggca tttcttcccc atttgttctt cggaacaacg ccccggaacg cagattcgtc
     1081 atcccgtagc gttttccata aaacagtttc tgccactgac ggaccatacc gagatattca
     1141 ttattaaaga cgcacagaat caacggaagt tcctccaaaa cagcagtcgc aaactcctgt
     1201 atattcatct gcattccgcc atcaccggaa atcgcgatca cggttctgtc cggattgcct
     1261 atcttcgctc cgatcgcagc ggggaatcca tatcccatag ttccgagtcc gcctgatgta
     1321 acgagctgct tcttttccgt tatttctgca tactgcgtca caaacatctg atgctgtcct
     1381 acatccgttg taataatagc atcgtcaaac tgtctgttca tctcttcgat aatatctctt
     1441 ggtcctatcg aaccgtgaac tttcattgta agcggatgtt ctgcattcca ctgcgcgata
     1501 cgttctaacc actcttttgt ctcacatggt tccacatatt ctctcatctt cgccacagct
     1561 tctttcgcat cggcaacaat aggcacatgc acatgaatat ttcttgatat cgacgctgta
     1621 tcaatatcta tatgtacaat ctgtgcattc ggtgcaaatt catgaagttt ccctgtaatc
     1681 cgatcattaa aacgagtacc gatagaaaac aacagatcgc attcgctcac tgccatattc
     1741 gcggcatacg ccccatgcat tcccaggttt ccgataaaca gcggatgatc tgtcggaacc
     1801 gcgcctcgtc ccatgatcgt cgtcactaca ggaacgtttg ttatattgac tacttccgta
     1861 aactctttat tcgctcttgc gatattcact ccgcctcctg caagaaacag cggttttttc
     1921 gccttattga gcatcttaag cgcgcgttta agctgcccca tatgaacgct cgtattcggc
     1981 ttatatccac gaagatttac cgtttccgga tattccgggc ttccaagctc actcatcaca
     2041 tctttcggaa ggtcgatcag cactggtccc ggtctccctg tccgcgcaat ataaaacgcc
     2101 tctttaatga tccttccaag atcttctctt ttactcacgg tcacaccgta ctttgtaata
     2161 cttcgtgtga ttcctacgat atccacttcc tgaaacgcat cgtttccgat cagatggcgg
     2221 gccacctgac ctgtaaaaca aactaacggc acactgtcat aattcgccgt tgccaacccg
     2281 gtaacaagat tcgtcgctcc cggaccgctt gtcacaaggc acacgccgac tttccccgtt
     2341 gtccgcgcat atgcatctgc cgcatgtaca agcgcctgtt catgtcttgg caagatcact
     2401 ttgatctcat cttgtctgta taactcatcg ctaatatcaa tcgtacacgc tcccggatat
     2461 ccgaaaatcg tatcgactcc ttcttctttt aacgctctta cgagcagctt atttcccgta
     2521 atctgtctca aaatcttacc tcctcaaact aagggtctgt ctctcatact ctcattgcct
     2581 gaaaaaacaa caactccgaa tccaaatttt ctcttccggc atcttaagca atatgatact
     2641 ttgtatccgg tatcttgtca atttcctatc cttgatcttt tttatctatg gcagcagttc
     2701 agccatacag acgtccgccc gttcatgagt aaaacagcag caccggccac aagaagcacc
     2761 cgtgcggttt tacgaatgac gcagtcggaa ctgttacttt ctgcatcgta tcattccgac
     2821 aatagaatac accgctagag ccgcagcatt tacgatgaca acacttgctc ctgcgggagt
     2881 tgcccataca taagaaatcg caatcccgat cacaagacat aaaacagata ttacggcgga
     2941 atttataatg acagaccgaa atgttctgca tacacgcatc gacgtcagcg ccggaaagat
     3001 gatcagactg gagatcaaaa gagctcccat catccgcatc ccaagcacga ttgtcacggc
     3061 tgtcagcaca gcgatcagcg tattgtacag attggctttt actcccgttg cctgcgcaaa
     3121 tgtttcatcg aatgtgaccg caaatatttt attataaaaa agaagataca aaacgagaac
     3181 gattcccgcc aaaataacac tcagccgaac atcttcggcg ctcatagcca aaatactgcc
     3241 gaacatataa ttgtacacat ctgtattcat tcctgtcgtc atggaaatca ccatgactcc
     3301 aagggcaaga gaggttgtcg aaataagcgc gattgccgca tcacctttga tcttgccgtt
     3361 tccctttatc cttaaaatca aaacagcagc aagtacaaca accgggatcg caacagaaag
     3421 cggagctgcc tgaaacgcag ttgccaccgc aagagcgcca aaaccaacat gagacaagcc
     3481 gtctcctatc atcgaatatt gtttcagcac aagactgacg ccaagcagcg cggcgcaaag
     3541 ggcaactaat gaacctaccg caaacgcccg taccataaac ggataagaaa acatttccac
     3601 aatcgtatca atcatgctct tcacctccga ggaatttctt tccgatccgg cttttacgat
     3661 agtcttctgc cgttccgaaa aatcggaccg tctcctgcaa atgaagaata tgacttgcat
     3721 tttccactgc agtttctata tcatgagaga ccatcacaac cgcgatattt gattcctgat
     3781 tgatctttcg gatcagatca taaaattctc ctgttacgat cggatcaagt cccgtcacag
     3841 gttcatcaag taaaagtagg cttttcgtcg cacaaagcgc ccttgcaaga agcgctcttt
     3901 gctgctgtcc gcccgaaaga tcccgaaagc attggcgcgc caaatctgtg attcctaaca
     3961 tttccatctt ttctgccgca gcttctttat cttctttcct ataaaacggc ttccacccac
     4021 ggccgtttaa tcttcccgac aaaacgactt cataaacgct ggcaggaaat cctttttgct
     4081 cttcattttt ctgcggcaga tatccgatct catttttctt cagtccgtct ccgtatatga
     4141 ttttcccccg gtctgcgcct tttagtccga gcaggctttt gacaagggtg cttttacctg
     4201 atccattctc tccgacaatg cagaggtaat ctcccctttc aatagaaaaa ttcaattctt
     4261 ttacaaccgt ttgtccttcg taaccgatgg aaacattttc acacgttatc agcgccatca
     4321 gttcaaaacc tcctttaacg tatctgcgtt cttttgcatc atcgtaagat atgtctcgcc
     4381 tttttcaaaa tcttctgccg aaaggttatg gcagctataa aatattctca cttccgtacc
     4441 ggaagcttcg gctaccgcat ttgcaatgtc tgcattgctt aactccattt taagcactgc
     4501 cggaacgttc tcgtcttttg ccttttcaat caaaaatacc attgtcgccg cgctcggctc
     4561 tgtatcacct gcgcatcccg gaaatgccgc ataatattcc aatccatact cttttgcaaa
     4621 ataaagaaac ggaaaacgat ctccgaatat taataaattt ctttttgact gtctcacaac
     4681 ttctcgaaac cgcttatcca attgtgcaag ctctgtctga tatctatctg cgttttcctc
     4741 atacgccgct ttattttcag agtctttttg aatgagaact tctttgatct tctccacgat
     4801 cacagatgca ttgacaggag acgtccatac atgttcgtca atttcatgga cagatccctc
     4861 atgttcctct tcaccgtgca tttcatggtg agagtctcct gccggttctt tcattccttc
     4921 tacatgttct tcttcctccg tttcaacaca atcgagaagt cttatcgaaa cccggtcttt
     4981 atccggcatg gaacttaaaa tatcttcaac ccacacatca ttttccccgc cgacataaat
     5041 gaacacgtca ctgttttcaa tagcgatcat atcttgcggc gtcggctcat aagaatgact
     5101 ttcctccccg ggttttaaaa gcatttttaa gtctacgcag tctccggcga tctcccttac
     5161 aaaatcatac ggaggaaaaa ttgtcgtcac aacactcagc cgcccatttt catttgtttt
     5221 gttttgttct ctatgccccc cgtctttatc agccgggtaa cctgcacatc cctgcacaat
     5281 aacagcaagg cacagaacga gaaccgccgg tccccgtctg cgccatattt ttttcttaac
     5341 atctttcata tgttgtaaaa caaaattcca gatcaaaaca agtctgctcc tcactttcct
     5401 gcgtcatctt ccactcgctc atctcatcca aattcgggaa ataagtgtct gcctgaaatg
     5461 cacgatccac ttttgtcaca taaattttat cacaatacga aagcatcatc ttgtagacgc
     5521 tctctcctcc gataatgtag atttgttccg tatcatactt tccgagttca ttcatcaatt
     5581 cttcttcatc gtgaacgatc accgcacctt ttgcttcata gtttctgtct cttgtaagca
     5641 caatattgat cctgttttta agcggcatcc cattcgggaa actttccagt gtctttcgac
     5701 ccattacgat aactttacct gtcgttgttt ctctaaaaaa cttcatatca gacggaatac
     5761 tcacaagaag cctgttcttg tatccgattc cccagttttt atcggcacat acgattgctc
     5821 tcatactccg cacactcctt tctctactca aacagcaatc ggaatatttt taatctgcgg
     5881 atgcgtctca tagcgatcca gtctgacgtc atccggtgta aaatcataaa agtctgtaat
     5941 ctcaggattc agccagaacg tcggcgccgg aagcggttct ctggaaatca gttcctcgat
     6001 catcggaaca tgcctgtcat aaatgtgcgc atctgcgatc acatgcacaa actctcctgc
     6061 tttcattccg catacttgtg caagcatatg aacgagaacc gcatactgac acacattcca
     6121 gttatttgcc gcaagaacgt cctgagatct ttgatttaaa atagcattca gcgtcaattt
     6181 ctcactgtct ttttcttttg tcacattgaa cgtcatacta tatgcacacg gataaagatg
     6241 catctcatgc aggtcattat gaacataaat gttcgtcatg atacgtctgc tgtacggatt
     6301 atttttcaga tcaaatatga cacggtccgt ctgatccatc cggccttctt tatactgatg
     6361 cttcactccc atttgatagc cgtaggcttt tccgatcgac cctgtctcat ccgcccaact
     6421 gtcccaaatg tggcttttta attcatttac attatttgat tttttctgcc agatccaaag
     6481 cagctcgtcc acacagcttt ttatcgctgt acggcgcagt gtcaacgccg gaaattcttt
     6541 cgataaatca tatcggttca caacgccgaa tcgtttgatc gtatatgcag acgttccgtc
     6601 ttcccagacc ggacgcactt tttctccttc tgtgctcgta ccgttgtcaa taatatcacg
     6661 gcacatatcg ataaacactt tatctgcgta actcatatat cttctccttt tatcaattcc
     6721 ttttccgctc ttccggctca tttgcttccg cctgttctca cggatctgca atattattca
     6781 atatcttatc caaattcttt ctaagagctt ctgtttccct ctttgtcatc cctctcgtca
     6841 gttctctgtc cagcttgcgc ctgtcagctt ccatttttct tctaatatca taagcttttt
     6901 ccgtaagata aattatcttt tttcttctgt cgtttgcatt cggtacacag aaaatatatc
     6961 ctttttcatc taagcgcttc agtattccgg tcacagtcgg atttttcaga ttcagatttt
     7021 tctccacatc tctctgactc acttcatctt tactcgtatg aaacaaataa tcgagcaccg
     7081 cgcactggga tgatgtgatt cctatttttt tcaatcgttc attaagatct ttttcatata
     7141 cattattgac ctgttttatg ataaaaccga tcggcaaact ccgattttcc atctgcactc
     7201 cttattcgga ctttttgaaa attcaactgc tcccgcccgg catctaaaat ccggcggcag
     7261 tttctatcaa tactctctga tcatgctgct cacttcaaaa atacttttca tttccttcaa
     7321 ctcacaaagc gcgcttccca tatgtttttc tttcactttc tccgttacga tcacaagctc
     7381 cgctctgtcc gtcttggcgt ttttctgtac gactcttgca atactcacct tatgttcccc
     7441 gaacaccttc gcgatctgcg ccagaacacc cgggcagttt tcaacctgca tcctaaggaa
     7501 gaatttattt ttcacatctc cgaaatcttt tacggcaagc tgctgataac atgtacagct
     7561 tatccgtccg ctgcatccgt attcaatatt tctcgcctcg tcgatcacat cccctacaac
     7621 cgcgcttgcc gtcggcatct ctcctgcgcc tcttccgtag aacatcgcat cgtccactgc
     7681 atctccgtga ataaatacag cgttaaaaga atctcttaca gatgccagcg gatgatctct
     7741 cgcgatcatg accgggtaaa ctccgacctc gattccttcc ggcgtatttc tcgccacacc
     7801 aagcagcttg atcacgctgt caaactcttt ggcataggcg atatctgccg ccgtaatttt
     7861 tgtaattcct tccgtataca catccgcaaa cacgactctt gaatgaaatg caatagaagc
     7921 catgatcgcc atcttccttc cggcgtcaag accttctacg tccgccgtcg gatctgcttc
     7981 cgcatagccg agttcctgcg cttttttcaa agcatccgaa aattccattc catcctcaaa
     8041 catcttcgtt aaaatatagt tcgtcgtacc gttcacgatt ccgagtatgt ctgtcaggtc
     8101 gttgcccgca agacactgtt tcagtggtcg gatgatcgga ataccacctg ccactgccgc
     8161 ctcaaacaga aaatcttttc ccgctgcctg tgccgtgtca aaaatctctt ttccttcttc
     8221 cgcaatcaga tctttatttg cagacacaac gtttttccct gcttttaaag cctccagtat
     8281 catcgttttc gccggttcga taccgccgat tacctcaatt acgatctgaa tctcatcatc
     8341 ttctataatt tctttccaat catctgtaag aagcgtctca tcgactcctt ctcttttctt
     8401 attcttatta tggacaagaa tctttttaat ctcaagctgc gcaccgatcg tcttatccat
     8461 cgtctccgcc ctcttacgaa gaagcttgta cacacccatt ccgacagttc caagtccgag
     8521 aagtcctact tgtattttac gcatatcgaa acctcacctt ttctttccgt tcttttataa
     8581 atctaatctc tgagatacat gttatttatt atactttgaa aaaaacaaat tgtaaaagac
     8641 tttttaacgt atatcatcta ttatatagta taaaataaaa agcactcgca tctcatctga
     8701 gatatcaagt gctttttaag agcgacagac ggggttcgaa cccgcgaccc cgaccttggc
     8761 aaggtcgtac tctaccagct gagccactgt cgcattttct ccgttccgtt gaccggaaca
     8821 atacatacta tactacgcac aaagagaaat gtcaacacct ttttttattt ttttatcata
     8881 aattgcattc atggattgta ttccaaaaat tctcaaaact tttttatttt ctcattactt
     8941 ttaaagagtt ttcagctcca tatattttcg cattgcacct cacccacttc gatgctatac
     9001 tttttatgta tttgtattac tcagaaagga gtcctatcat tatgaaattt atatatcctg
     9061 cggtcttccg caaaaacgaa agcggcgggt acgatgcata tttcccggat cttgaatgct
     9121 gcgaagcatc gggagatact ctcgacgatg caatcgacaa tgcaaatgaa gccgcccgca
     9181 actggatcat ggtagaattt gaagaagaaa atcctgtctt cccatatatt tcagatatca
     9241 atgatattga aacagaggcg ggcgatatcg ttcggaatat ctcggtaaac atccggttct
     9301 atgaaggctg ggatgaataa agacgcatcc ggcgatatac gctgaaattc gtttagctat
     9361 aataaaataa aacctcactg caaaaacgta ccgggctttg atctgacttt ttcaaagcag
     9421 tacagttaag gcagtgaggt ttttattctt atttttctgt ttcggctgtt gtttcaagac
     9481 caagttcatt cagctgtttt acatcaacga cacttggcgc ttccgtcata aggcaagacg
     9541 catctttcac tttcgggaat gcgatcacat cacgaatgct gtcctgtttt gccataagca
     9601 taacgagacg atccaaaccg taagcaagtc ctgcatgagg cggaactcca tatttaaacg
     9661 catcaagaag gaatccaaac tggctgtatg cctgctcttt tgtaaagccg agcacttcaa
     9721 acatcttctc ctgaatatca tcctggaaga ttcggacgct tcctcctccg atctcatttc
     9781 cgttcaaaac gatatcatat gcttttgcgc gcacttttcc cggatcgctg tcgatcagcg
     9841 gaagatcttc ctccatcggc atcgtaaacg gatgatgcat tgccacatag cggttctgct
     9901 cttcactcca ctcaagaaga gggaactctg tgatccatac aaatcggaat tcatttttat
     9961 caagcagttc aagctgtttt gccagttcca cacgaagcgc tccgaggctg tcccacacaa
    10021 ccttgttctt atctgccgca aaaagcagta aatcaccggc ttctgctccc atcgccttga
    10081 tcagcgcatc catctcttct tccgtcataa attttgcaaa ggaagatttt atacttccgt
    10141 cttcctggat cgcaatataa gcgagtcctt ttgcgccgta atctttcaca aatgccgtca
    10201 gtttatcaat cttctttctc ggcatcccgc cctgaccttt cgcgttgata ccgcgtacac
    10261 ttccgccatt ttcgactgct ccggtaaata cggaaaagcc gcagtttttc acgacgtctg
    10321 tcacatctgt aagttccatg ccaaaacgaa gatcgggctt atccgaaccg aaacgatcca
    10381 tcgcttcctg ccatgtcatt ctctggatcg gaagcggtac atctacgccg agaacttctt
    10441 taaatactct cgcaagataa cgttcattta catcgatcac ttcatctaca tcgacaaaag
    10501 aaagttccat gtcgatctgt gtaaattccg gctgtctgtc cgcacggaga tcttcgtcac
    10561 gaaaacatct tgcaatctga atataccggt caaatcccga acacatcaaa agttgtttat
    10621 aaagctgcgg cgactgcgga agtccgtaaa aacttcccgg atgcacgcgg cttggcacta
    10681 aataatctct cgcgccttcc ggcgtacttt ttccgagtac cggcgtctct acctcaagaa
    10741 atccttcctc tgagaaaaac tgtcttgtca atgtcatcac ttgacttttc atcatcagat
    10801 ttctctgaag atcggctctt ctaaggtcaa gatagcgata tttcaaacga acttcttctt
    10861 ttgtcttaga gttttcctca atcggaaacg gcggtgtcaa agcctctgaa agaattctga
    10921 gttcacttgg gataacctcg atctcccctg tcgcaatctt atcgtttaca gcgccggaac
    10981 gcattgccac ctctccgacg atcgcaacga catattcgga acgaagtcct gctgccttct
    11041 cgatcagcgc cgcatctgtc ttgccttctt cacatacgac ctgaatgatt cccgaacgat
    11101 cacgcacatc tacaaacaca agcccgcctt tatttctgtt cttttgaacc catcccataa
    11161 tggtcacttt ttcacctgcg tttgccgcag ataattctcc acatctgtga gttctcttaa
    11221 gtccctgcat tgattcagcc attgtacacc tccgtaatat ctgtatatcc ctgttctgca
    11281 agctgttcct tttggaactt cttgtttttc ttcatattta ctatcataac ctgtctgcct
    11341 gcttcccgct ctttgcgcgc ttcttcaagc acctccagaa gtttctcctg cggaagtttt
    11401 ttctcgatca gaaacgcttt tttcggtttg ctcgtcggca cttcgtatcc ccgttccaac
    11461 agcagcatga cgatccgctc aaacccgatg gaaaatccaa ctgccggcgt atcctgtccc
    11521 gtaaattttc cgatcatctt atcataacgt ccgcctccgc cgacgcttcc gccgaattca
    11581 tccatggaaa tctcaaagat tgttcctgta taataagaca ttccccgcac aagcgtcggg
    11641 tcaaaactca tcttaaattc agcttctttc tgactctcca cactcgtgat gatcatttcc
    11701 agtgaatccg ccgcctcagg cgcaagatag ccttgtaatt tctctttaca gagacggacg
    11761 ccttccacat cattcgtgat ctcttcaaaa agagcgagat atttttccac cgattcttcc
    11821 gcatatccgt ttcctttcag ctctgccgcc acgccgtcca atccgatctt gtccatctta
    11881 tcgagcgtta taaacacact gtcataatct tcctctttga atccgctgta tgcagccatc
    11941 gcctttaaga aacggcggtc attaatacgg atcgtaaagt ttttaaaatc caattttcca
    12001 agcagcgccg ttgtggcaag gatcagttcg atctctgcca gattcgacgg ttctccgaga
    12061 atatcgatat cgcactgcat gaactgacga aaacgtcctc tctgcggacg atccgctctc
    12121 cacacatttc ccatctgaag tgccttaaac ggagacggca gttcattcgc atgatttgca
    12181 tagtatcttg agagaggaac agtcaggtcg taacgaagtc ctccgtccac gagatctgcc
    12241 tcttctttcg ctgtatcaag ttttagcttc tcccctcttt ttaaaatctt aaaaataagt
    12301 ttttcattat ctccgccctg cttactgcat aagttctcga tatgttccac acacggagtt
    12361 tccatagagg aaaacccaaa tgttttatat gtctctttga taagaccgat aacataatcc
    12421 cgaacttcca tttccgccgg catcatgtct ttcatgcctg taaccggttt ctttttcagc
    12481 gccataacca ttctcctttt cgttctatca tttcatcgac cctgtcgata tattccataa
    12541 tatttttcac gttttcacag cgaaccagat taatctcttt cccgctatct gtccggatcg
    12601 gatctttgag taatatcttc gtcgacgctc ccgctccggc tgccagtatc gtctgttttt
    12661 cttccataat cagtatattg tatattccgg ctttgtcaag ttctgcataa ccgacatttt
    12721 caaaatttcc cgcaatattt ttctgcctgt ataaataata cggctgcagt ccaatccgct
    12781 tggcacaatc agctgccgac attatcatct gcgaaatctc agaatgaaga tcctgttttc
    12841 ttccctcctg tccgtatctc gccgctcttt tgatcgccag cgcatgaatc gtcagactgt
    12901 caggatgcat ttcttccacc tgccgaagcg tatcttccat atcagaagca tcctctcccg
    12961 gaagcccggc gataagatcc atattaatat tgtcaaatcc cagtttcctc gccagataga
    13021 atgcatcttt tgtctgtgcc accgtatgat ttcttccgac cagatctaat gtcttttgct
    13081 gcattgtctg aggatttatt gaaatacgag tcaccgggta ttttctgatc accgcaagct
    13141 tttcttccgt aatgctatcc ggtcttcctg cctccactgt aaattcaagt acgtttgaca
    13201 agtcgaaatt ctccgtgatc gtctttaata aacgatccat ctgctctgcc tccaaagtcg
    13261 tcggcgttcc tcctccgata tacacgctgt ccggtctgtg cccttccgac attttcccga
    13321 ttgcccggat ttctttcaac aaagcaaaaa ggtaactttc caccttatct ttccactctg
    13381 cgatcggcga agaactaaac gaacagtaag agcatacgct tgggcaaaac ggaatcccta
    13441 catataaact aaacccatcc ttataatcca atttggacaa aagtttcttt tccctcacag
    13501 caatctcaca tgcaagctgc gctttctctt cactgacaaa aaattcatca cgaaacgctc
    13561 cgactgtcct gcggactgct tccgcttccg tcatcgattc atattgttcc aattttgtca
    13621 tggcaagttt tgtcggccgc actcctgtaa gtgttcccca tgcaagcgtt gtccttgtct
    13681 gttgttcgag atatcggtac aacaaacgag tcagttcttt ttttattgcc tgttctttcg
    13741 cctcttctgt ctccatttcc gaaagatcac ctgctgccgc ttccacttcc tcaagcgcaa
    13801 tggaaaagca agaccctccg ggcagattta atgccacaag agattcctgc ttttcatcta
    13861 cattctgtat gatctcttca ttggggaaaa aggcttttac aagatgatat acattgtatg
    13921 tatatttatt tttcttactt gatactttaa tcatcgtctc ttcccatttc ctattatctc
    13981 tgcgaagcct attccatcgc cacctgttat atccattcct atattccaat cattcccgtt
    14041 cacttattaa ctttatatga aaggattata tttcttttca tgcgaaatcg ttgtctcttc
    14101 catatgtccg ggatatactc tcgtatcttc cggcaaaacc agaagttttt ctcttaccga
    14161 acggacaagc tcacttccgc ttcccgtagg aagatccgtt cttccgatag aattatgaaa
    14221 aagggtatct ccgctgaaca atacatgttc ttctccgata taataacagc atccgccctc
    14281 tgtatgtccc ggagtcggaa taatctccac ctgaaagcct gcggtttcta tctgcgtatc
    14341 cgcttttaca tactctgctt ttgtaaacac atatgactgt ccgagcatcg tcgccgatgc
    14401 attcagcacc ggatcgcata aaatattttc ttccccttca tatgcataaa ccggacatcc
    14461 gaagatttcc gcaaagcgtt ccgctcccat aatatgatca aagtgaccat gcgtcaaaag
    14521 cagcgctttt acgcaaagtc ctgcatcttt tatatgcttt acgaattcct gaggacaatc
    14581 cgccatatct acggcaaaac attccgaagt catctcgttt attacgatat aacaattcgt
    14641 tcctacaggt ccgaatacaa atttctcaat gcgcatgaac tgtctctcct ttttatcaac
    14701 tctgactttg ttcaaactgt cattttactc ccgccgcctc cgcaacggga acgctattgc
    14761 ttcttacttg aaaggcttca tataagatca acccgtcgtt ctctcaatat caagaactcc
    14821 ctgaagctga cgcagtttct ctatgatcgc atggagttcc tctcttccgt ttacaataaa
    14881 tcccatatcg atcgttgctg ttcccttttt gcttgtgcga acattcatag acttcacatc
    14941 tattttcgct tctgtaaata cttttgagat atccataagg aatccttgtc tgtcgtttgc
    15001 gtacatctta agctctgcca gatattttcc gccgccctct gtctcggacg tatcttccca
    15061 ctccgcgtcg atcaaacgcg ccctctctac atcagaaagg tggagcatat taatacagtc
    15121 cgtccgatgg attgacattc cccgtcctct cgtgacaaat ccgacgatct cgtctccggg
    15181 aaccggattg cagcattttg aaaaacgcac tgccatatca ttaattcctt ttacaacaat
    15241 accgctcttt gactttgcaa tgtgaacttt ttgagagttt gcttctgaga ttctctcaag
    15301 aatcgtctcg tctgtaattt ctttcttatg ttctttttcg tactcctcaa aaagtctgtt
    15361 tacaacctgt ccttccttta aaccgccatg accgatcgcc gccaaaacag cgtcccaatc
    15421 cctgaagccg tactttttct gaacaacctg ctgatacttc ggtttcagta tattttggag
    15481 attaattgat ttccccctgc aataggaaac gatcaattct tttccccgga taatattatc
    15541 ttccttaaac tcttttttga accactggtt aattttattt tttgcctgtg tactcttaac
    15601 gatattcagc cagtcgcggc tcggtccttt tgaattctgc gacgtaagaa tctcgatcct
    15661 gtcgccgttt tgtattttat aatctatatt gacaagtttt ccgtttacac gtgcaccgac
    15721 catcttattt cccacggcgc tgtgtatcgc atacgcaaag tcaaccggtg tggaaccgtt
    15781 cggcaaattc ttcacgtcac cgtttggcgt aaaacagtaa acgtcttcag caaacagatc
    15841 aaggtcgcct tttaacaggc ttaaaaactc ccggttatcc gacatgtctc tttgccattc
    15901 caaaatctga cgcagccagt tcagcttagc ctcctgagat tccatactct tcgctgaatc
    15961 tccgccttct ttatatttcc agtgagccgc aataccgtat tcagccgtct tatgcatctc
    16021 ttctgttctg atctggatct caaacggctg tcctgacggt cccatcagcg ttgtgtgaag
    16081 agactgatac atattcggtt tcggcatcgc aatataatct ttaaaccttc cgggaatcgg
    16141 cgtgtacatc tcgtggatta ctccaagcgc cgcataacaa tcttttacag aatcaacaat
    16201 tatccgcacc gcaaacagat cgtatacctg atccagcgtc ttattttgat tcaccatttt
    16261 cttataaata ctgaaaaaat gctttacacg tccatatact ttcgcctcta tatgcgcatt
    16321 tttcatatgt ttcgaaactt cggctactat ctgctgcaca aattcctcgc gttctgtttt
    16381 ccttcggttc agatccctca cgagattata ataaacttcc ggtttccagt attttaaaga
    16441 caaatcatct aattctgtct taatcttgga aattccgagt ctctgcgcga tgggagcata
    16501 aatatccatc gtctctctcg ctttttcctg ctgctttgtc ggagtcataa attcaagcgt
    16561 acgcatatta tgcagtcggt ctgccagttt aataataata acccggatgt ccttcgccat
    16621 cgcaagaaac attttcctga ggttctctgc ctgaacttct aatttgtcct gtgaataatt
    16681 caactggcca agctttgtaa cgccgtccac aagaagagcg acttcttctc cgaactcacc
    16741 tgccacatcg tcaagcgtca tatccgtatc ttctactgcg tcgtggagca ttcctgccac
    16801 gatcgtctct ttgtccatct caagatccgc caaaataata ccgacccaga gagggtggat
    16861 aatatacggc tctcctgatt ttctcacctg atcacgatgc gcttctttcc ctatacgata
    16921 agctttttcg atcatggtaa tatccgcaga cggatgatac tttctcactc tttcaataag
    16981 aacatgataa agctgctccg gatcttgata atcctccggc gcttttactg catgaccgtc
    17041 cacgatctcc agatttttat tcaattcttc atattctgcc gttcctgcca tagtaaagtc
    17101 cccttctctc atgcagctca ttcaacaaag ttataagatc gtttaattat atcatttctt
    17161 ttgataattt acaatcgtaa gctgtatgct tcgtctgccc atatattcgt taattgacgg
    17221 aaaataagta accgcggtga cttttcctgc cagcatctct ttcaggcaag tctttacatc
    17281 gccgaaataa accgctttca tacgatttcc ctgcttatct tccaatatac actgcaaaac
    17341 attctcgttt ttccctacaa ttttaggttg aatcaccttc agatctttct ccgcaaacag
    17401 cggtctttta tttccttttc cgaaaggttc catcacctga aactgctcga tcaaatcttc
    17461 tgttaaatac ggaaaaggaa cttgcatatc gatatatact ttcatttgaa ggtcttcttc
    17521 tgtcaatgta caaagagaat tgatcgtttc tctgaatcgc tgaacattct cctctttcag
    17581 ggacacgcct gccgcctgcc tgtggccgcc gaattttgta aacagcgcct gacatttaca
    17641 caattctgta aacatgtcat actcttccgt agaccgtccc gatcctttca ctccgtcttc
    17701 tgcctttgtt aagatgatcg taggcctata atatctgtct ctgatctttc ctgcgataat
    17761 tcccgcaata ctttcatgac agtccggcag atagatcaca aatactttgt cctctttcag
    17821 cgaggtagtt tctatcatct ctgccgcttc tttaattcct cgttccgtca tttccttacg
    17881 gctgtcattt aacgccttta aatcttccgc caacatgacc gcatcccgcc tcgtccgtgt
    17941 actcagcagt tccaatgctc tttttgcggt gtccagtctc ccgctcgcat taatacatgg
    18001 tccaagaaca aacccgagat cgtatgtatt caaatattcg ggattgattc ccgtacattc
    18061 tataagcgct tttaaccctt cattttttgt gcgcttgagc atctcaagtc cctgtttcac
    18121 aaaaatacga ttttctccct ttagcgccat cacatcacct actgttgcaa tcgccacatt
    18181 ttccataaga taatcaacgt cttccggatc tcggagcata acattataca atgcctctac
    18241 taacttatat gcgaccgctg ccccgcacag atttttaaaa ggatactcac aatccggacg
    18301 ccttggatct acaaccgcat cagcctgcgg caggagatat tcccgttctt tgtttatttc
    18361 tacaaaagga acttgatgat ggtctgtcac gatcaccgta agaccgtttt ttcttccgta
    18421 agtgatttct tccgcagccg caatcccgtt atcacatgta ataatcgtat cgatcccatc
    18481 atctaacgcc cggtcgatca gcattttatt taatccgtat ccgtctttaa tcctgtcggg
    18541 aatatctgta tcaacatctg ctccgagtcc ttccagacct tctttcaaaa tataagctgc
    18601 attcacaccg tcaatatcat aatctccgat cacccttacg ggtttttctt ccctgatctt
    18661 ctccgataag atctcaaccg ccttatccat gtccttcatt aacattccgt catacaggtc
    18721 ggcaatcgtt ccgttcaaat aaagattaat ctcttcatct tctataatat ctctgtttct
    18781 gatcaggcgg gcaatgaccg gcgaaatatt atatttcgct ccgatactgc tgaaatcagc
    18841 tttcttcatc attacatacc agcgttccat cgtttgttcc tccaagtata tcacgtacat
    18901 ttttgtatta tagtatcata attttcaaaa tatcaccata gagaaaaccg gccgtaaaac
    18961 gtttccgctt cacgaccggt cttttcattt tcgtttcaaa caatctttta acgaaaattt
    19021 atcttctct
//
LOCUS       DBMDXF010000010        19003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_896_length_19003_cov_2.143424, whole genome shotgun sequence.
ACCESSION   DBMDXF010000010 DBMDXF010000000
VERSION     DBMDXF010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 19003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19003
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_19003_cov_2.143424"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3419)
                     /locus_tag="ACZPD4_00880"
     CDS             complement(<1..3419)
                     /locus_tag="ACZPD4_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1377665.1"
                     /translation="MGGGTGQGDQDYPHNVVITFGKDVTFQSFSYTPRKEGENTNGNI
                     KGYKLYASTAENKLDYESEDWGEPIAEGEFEYNGTNPIYVNLKEACTAKQIKFVATSS
                     NNGERFAGGAEFNLHADKAPVDTDDRAFETSDLELKDGNEAVKVEDTTATINGKEKTG
                     KKVTFSFKPYTHKNVEYSIDEVIVMYEGDHFMRKFLEIDVPDDKMADAEIDYIDLESL
                     KVAESDAQWTIPRGQGGVVQMEEFKANLGQPIYIQGMFFGCEFPAADTEIVNGTGFMR
                     YYSGKTFSRLKEDNQLTTDDKYVTWQTVAGAARSTEQEVIQADFFEYIKSIATPSEFR
                     TQYNSWFDNMMKISDENILASFIEIDRELNKAEVRPLDSYVVDDGWNAYNDGSIGAGS
                     HAQSGAIENTEGFWTFNEKFPEGLTPSSELVKKFGSNFGVWVGPRGGYNFYTTLANII
                     ERAQKGSKAGHSIDVADRVYVENFKKMAIKWQQDWDVNYWKWDGFADTAQYNHFNNLG
                     GADGVPVYSESNHHMTGGYHQMYHVTDLWEAWIDLMEAVRQSEKEDGINKLWISLTCY
                     VNPSPWYLQWANSVWIQCVHDQKDASFGTTKMNKQITYRDACYYDFLKNHQFQFPLQN
                     LYNHDPIYGKEGTGMTVNTATDEDFQNYLYMLSTRGTAFWELYYSDSIMTDGKYEITG
                     EFLEWAEENYHMLKNSKMIGGKPDITKLSNGDLSDQTQAEAYGFSCFDGTDGIISLRN
                     PSANADKTIKFTFDRTMGVAEGAGTLNYYLEHSYLLSDKSAQTGTLKYGQEYTVNLKP
                     NEVRILRVSAEKDTTAPKIDRIMTDGAKELTVKFDEKVSGNLFKVENAKVSSIKKSAD
                     DTTYHIVLAEAPANEATVKVIPQDIKDMSGNKATEAASVVYHKDSVIVEKEAIAEAGE
                     IAAADRSLNSNNGFTVYAAVNTTATDKSLVSQNGQYELKVTAEGKASFTLNGATAVSG
                     KSINDGAEHKVVGVKENNGMLKLYVDGTLEGSAYNEKNRFHEVKKAAITAGEGVTAAA
                     VYDIAYGYNEVANLGEQEGLPKLKLTNDMITVSETSEGSKDKVLDGDNTTYWTSQKVE
                     EGTVSSDNAWLQVDLGATYKLDQVDYTPRYYNDAKNYWHCTGNIKNLIVEI"
     gene            complement(3394..3864)
                     /locus_tag="ACZPD4_00885"
     CDS             complement(3394..3864)
                     /locus_tag="ACZPD4_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377666.1"
                     /translation="MKKKFFSAALAAAMVVTSVFSTTSVAGAAENETAVPYGKVTVEQ
                     KDNTVTIGNDAIKRTFSTADKKLSTTEIVNKRTGGEGTTFTPQEGSEEFVVKTTKEQK
                     GSITLEAINRDGWTATADSYQNASGDSDGPASNLLDGRTESIWHSNYGGGDWTR"
     gene            4143..6749
                     /locus_tag="ACZPD4_00890"
     CDS             4143..6749
                     /locus_tag="ACZPD4_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1377667.1"
                     /translation="MKKLIYPAALILLFIILTTTILPADAVYGSNTDWFSQHAALAET
                     IRDACVEQHTLLPDWIDLGSGSNGYQFSYYGFLRPDILIGCLFPQIPMVYILISYMLA
                     LYLASVLLCYMWLQSEDISPFFSFIGSVLFLTAGCLFHMHRQIMFVNYLPFLILAFLC
                     VKKRKFRFLPLCMLLICLHSFYFAIAAFAAIGWYWLRTEKETFWKDSFFKKYIPSALI
                     SVCMAAALLLPTGLVLMEHRRSGGGFSLLKILELFGPNISMNGILFNEYGLGLTLICF
                     YTILAGLNKKNPAKKEFFPDSVLFLSFGLFGIFSYILNVTLYARPKILIPFLPLFILH
                     CVRYLKSAHTETDRISSYPLYPFAVMLPLGLLWFSQVQFPWILIELGILFLFCLIQRR
                     KQNLILQMFKNPRIFARVELLVYLLILIAPVGMYITTARKENWVKKADVSAKETYTKL
                     AEIPMDPLYHFDSLTEPLANGNRAPEAKITRSSMYSSVTNSAYSDLYYDVLNTPIRIN
                     NRIALLTSDNPFMLHLLGVRYIETEKVHIPAGYTPLYSSAKDTVVAENKNVLPNVYFT
                     SDTISEKEFDRFNQIEQLEAISRKTIIENTPTDTDSDVYLPGKFITPFAPKLSADGKL
                     PDSLTIKKTADKYDIISKCQQSLTFYVENTGFGNILLLSFQVDNKTIDPVVIDINNIR
                     NKLSGLFAPYPNGNNTFHYQFSADSDSGMTKLKVTFPKGHFTVSNVQWHLCNKHIFDD
                     KNTITKAVCDSRTERSAFLSGTTVFSGSIHAESNGVLASAIPRQNGLELYIDGRRTDI
                     IRVNKAFAGAHIEKGTHKIEFRFSPPGKQIGCIISLISLLCYLSYLIFTFGVFTSRKT
                     QNITTAHHKNAL"
     gene            6790..7236
                     /locus_tag="ACZPD4_00895"
     CDS             6790..7236
                     /locus_tag="ACZPD4_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1377668.1"
                     /translation="MKRIYLSKELLTYIITGGITTGVNYMLYGLFLFFHVPYIASNSI
                     AWTGAVIAAYVLNRKWVFRSNRRIPEEFFSFAALRFITLLVENVLLWLAVDQLSLSLI
                     GSKLIVSVVTVIGNYALCKYRVFKTAAHGQDQNMIRKELLPYDNQK"
     gene            7220..8164
                     /locus_tag="ACZPD4_00900"
     CDS             7220..8164
                     /locus_tag="ACZPD4_00900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1377669.1"
                     /translation="MTIKNNPADDVISIIVPCFNEESVLPAFYSAVSDAASSIEGAVC
                     EFLFIDDGSSDRTAEILQHFTEKDERVRFLTFSRNFGKEAAMYAGLQNASGDYCVFMD
                     ADLQHPPALLKNMYHAVKYEGYDCCAGLREDRTGENPIRTFLSRSFYHLIGNACHLDM
                     GDGKGDFRMMNRAMTDSILELKEYNRYMKGIFSFVGFDTKWIPFHNTERAAGKTKWNF
                     RSLFRYAMDGMLSFSSAPASLPGFIGMFLLIAAVVTGLVSVFTGHKANWILLLVCLIL
                     ALNGIQMIFLSLLGQYMSKDYMESKKRPIYIIKKKGGF"
     gene            complement(8176..10074)
                     /locus_tag="ACZPD4_00905"
     CDS             complement(8176..10074)
                     /locus_tag="ACZPD4_00905"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377670.1"
                     /translation="MSKEKKPTKPSLNKNTLKTAKRLLGYVTKTYKVQFIIVLICILV
                     SSIATISVSLSLKFLLDDYIIPLIGQKSPDYTELYQALGILACIFLSGVIASFIYTRL
                     MVVIGQGVLKRVRDEMFEHMQKLPIRYFDRNTNGSIMSLYTNDTDTLRQMINQSIPQV
                     LMSALTIVVTFIAMLVLSPILTVLAVVMIGVMTAVARMVGGNSGKYFVRQQLDLANIT
                     GFVEERMTGQRVVKVFNHERISEEEFDQLNESLFVSSSKAHTFASIMGPVIGNMGNLQ
                     FVLTAVLGGALSVLGIGNITLGVMASYLQFTKSFTQPFMQIAQQFNSIIMALAGAERI
                     FNMMDEEPEVDEGYVTLVNAKKDAQGNLTECKERTGLWAWKHPHQADGTVTYTELKGD
                     VRFYDMTFGYTEDHMVLHDLTLYAKPGQKLAFVGSTGAGKTTITNLINRFYDVQDGKI
                     RYDDININKIKKADLRRSLGIVLQDTHLFTGTIMENIRYGKLDATDEEVYKAVELAHA
                     DGFIRMLPKGYETRLSGDGEELSQGQRQLLAIARAAIADPPVLILDEATSSIDTRTES
                     IVQKGMDNLMKGRTVFVIAHRLSTIRNSDAIMVLEHGRIIERGTHDELLSQKGTYYQL
                     YTGNLELS"
     gene            complement(10067..11800)
                     /locus_tag="ACZPD4_00910"
     CDS             complement(10067..11800)
                     /locus_tag="ACZPD4_00910"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377671.1"
                     /translation="MDKKLLRSVREYKKQSFLTPVLVAAEVFVEVLIPLLMAKIIDVG
                     IMNGDMAYIIKLGALLVLLAVVALFFGAKAGQLAAVASSGYAKNLRHDIFYKIQDFSF
                     GNIDHFSTPGLVTRLTTDITNVQMAYMFTIRLLARAPIMIVMSLIMTVTISPAIAFMM
                     LITIPVLGGLLIFIAKKAHPHFIKVFDEYDELNNVVQENVNAARVVKAYVREDHEVEK
                     FGKISGIVFRLFTKAEKIVAWNSPTMQFTMYIVVLAMVLIGGESIISGDMLTGELTSV
                     IVYALQILTSLMMVSFVFVMIMIAEASTERINEVLDEVPEMEDKADAVTEVTNGDIEF
                     EHVNFSYAGEGGNLSLKDVNLHIKSGQIVGIIGGTGSAKSTLVQLIPRLYDVTSGTVK
                     VGGTDVRDYNLKALRDQVSVVLQKNVLFTGSIYENIRWGDETASDAEVERVCKLAQAD
                     GFIREFPNQYETMIVEGGNNVSGGQKQRLCIARALLKKPKILILDDSTSAVDTKTDAL
                     IRQAFREEIPDTTRIIIAQRVSSVEDADVIIVMDKGEISGIGTSEELLKTNAIYREVY
                     ESQMKGGKDSE"
     gene            complement(11905..12387)
                     /locus_tag="ACZPD4_00915"
     CDS             complement(11905..12387)
                     /locus_tag="ACZPD4_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1377672.1"
                     /translation="MEERCDVGKIINRISHQLKRRMNCHEEEDSLTNMQRHVLHYILL
                     QSMSRDIYQKDVEAEFQIRRSTATGILQLLEKNGFILRKAEDRDARLKKIVPTEKAEK
                     VREHVLMNIDRTEKIIRQGIPDEKMAVCIETLELISANLFEDEKCCTEKQKTAAAGAV
                     "
     gene            12694..13833
                     /locus_tag="ACZPD4_00920"
     CDS             12694..13833
                     /locus_tag="ACZPD4_00920"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1377673.1"
                     /translation="MKQNNMLAMILAGGRGSRLHDLTQKVAKPAVSYGGKYRIIDFPL
                     SNCANSGIDVVGVLTQYESILLNSYVAAGRRWGLDAKDSGVYVLPPREKSDANLDVYR
                     GTADAISQNIDFIDTYSPEYLLILSGDHIYKMNYARMLAFHKESKADATIAVIEVPMK
                     EASRFGIMNTDETGRIVEFEEKPEHPKSNLASMGIYIFNWKLLRKTLLADMKNADSSH
                     DFGKDIIPALLNDEKKLCAYKYKGYWKDVGTIDSLWEANMDLLDKNNALDLNDPAWKI
                     YTEDVTALPQYIGADAVVDRAFITQGCVVNGKVKNSVLFTGAKVAEGAQVIDSVLMPG
                     AEVAEGAVVTRTLVADGVKIGKNAVVGSADSKNIELVAKRVKGDE"
     gene            13835..>14360
                     /locus_tag="ACZPD4_00925"
     CDS             13835..>14360
                     /locus_tag="ACZPD4_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1377674.1"
                     /translation="MNRAFGIINFAGNHIKVDGLQDFRPVGAFSFLGRYRIIDFPISN
                     MSNSGIDNIEVFIRRKPRSLTEHLGTGRHYNINSKRGSLNIMYTEHGQIGDIYSTDIT
                     AYMENIECLEEMPHEYVVIAPSYMVYKADYSEFLDAHIESGADISMMYHSVDNAKDAF
                     LNCNALNINKQKGVL"
     assembly_gap    14361..14370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14371..14962
                     /locus_tag="ACZPD4_00930"
     CDS             <14371..14962
                     /locus_tag="ACZPD4_00930"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR1377675.1"
                     /translation="AIEPNHGNAKNRNISMDTYVMKKDLFIELVRKASKRSSMYTFAD
                     ILDEESENLDIRAVSHRGFFAIINDFNAYYQANMSLVDLNNAGSLFDKDWPIYTHTND
                     SCPTQYFEGSKVTSSVVSNGCLIEGTVENSIIGRGCVIKSGAIVKNCIISADVVIGKG
                     VHAENLVVDKHARLIRGKRIIAAPEKPGYVKRGDKL"
     gene            15142..15879
                     /locus_tag="ACZPD4_00935"
     CDS             15142..15879
                     /locus_tag="ACZPD4_00935"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1377676.1"
                     /translation="MLKSYIAFDLETTGLDPMNNEIIEIGALKVRDGKVSERFMEFIK
                     PQELISPAITGLTGISNDMVASARPARSVITDFIDFCEEDILIGHNIIFDYSFTKCTA
                     AREGLPFEKMGIDTLKIAKKVHPELESKSLSALCEHYKIENKAAHRAYFDALATAKLY
                     QTLSHYFEPDNPKTFKPSQLTYKIRKPQPATPKQLALLERLTKNKKTFSHNTEAFSCN
                     AEALSYNPETLSRSDASRLIDRLLKGN"
     gene            complement(15883..17703)
                     /locus_tag="ACZPD4_00940"
     CDS             complement(15883..17703)
                     /locus_tag="ACZPD4_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR1377677.1"
                     /translation="MPIWKCNGKEKVNNNQKKRIYFKILNLLLVIILVFSVASVGGCS
                     MESKQGAKGAKSAGKRQSEDARFEEYTAELFCKEVSANTVSLHYTLKAPEKYGIEATD
                     ATFGQFEREPEAVKAAAENMRQALLGFNYDDLNVQNKITYDILNYRLKLMKKSADYTM
                     YEEPLGLVSGVQTQLPVVLSEYRFYDKEDVEVYLALVRQTEEYFEEIMIFEKKKAEMG
                     LFMPDQAVETVIGQCRAFLEMGNGNYLYSTFADRVMELNTLTEKEKSDYIQENALAIS
                     DYVFPAYERMTEELEQLKGSGKNEKGLVYFPKGKEYYELLARQSTGSRRSVEELKDLT
                     RRQINEDLTAMEQVLGLTTKEAKEAAAVITDKKAEQILEQLKEGSKTAFPEPPQTKLE
                     VKYVPEAMEEHLSPAFYMIPAIDNSQQNVIYINRARMGNDMTLFTTLAHEGYPGHLYQ
                     TIYYESTHPDPIRSLLDFGGYVEGWATYAEMGSYYLMGLSKEQATLLQKNASIILALY
                     ALADIGIHYDGWSVEDTEAFFKNYGITDKDAVGEIYKLILGSPANYLKYYIGYVEFLE
                     LKKMWVDEKGKDFLQKEFHSAVLKIGPAPFEIVEDYMWKM"
     gene            17844..18359
                     /locus_tag="ACZPD4_00945"
     CDS             17844..18359
                     /locus_tag="ACZPD4_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIR1377678.1"
                     /translation="MQLLVYISNYIVPFLILSIIVYGLSAKVNVYETFIKGAKSGFFT
                     VVRIMPTLIGLMAAVGILRASGFLDFISGLLENFTGRIGFPGELVPLTIVKMFSSSAA
                     TGLLLDIFKQFGTDSRLGMIASISLSCTETIFYTMSVYFMTAGIKKSRYTLAGALIAT
                     FAGLAASVLLA"
     gene            18570..>19003
                     /locus_tag="ACZPD4_00950"
     CDS             18570..>19003
                     /locus_tag="ACZPD4_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1377679.1"
                     /translation="MASVTLEHLSKIYSNGFVSVNDVSLRVDNGEFITLHGPSGCGKS
                     TILRMIGGLEDITSGKIYLSDELLNDILPRDRRLAMAFQNYTLYKHFNAYDNMALGLR
                     LRDFPRNIIDSRVNDAAKLLGISGVLGQKIKALSERDKQKVAL"
BASE COUNT         4919 a         4618 c         3613 g         5843 t
ORIGIN      
        1 ctaatttcta caatcagatt cttaatatta cctgtacaat gccagtagtt ctttgcatca
       61 ttataataac gcggtgtgta gtcaacctga tccaatttat acgtcgcacc caagtcaact
      121 tgcagccatg cattatcgct gcttacagta ccttcttcca ctttctgact tgtccagtat
      181 gtcgtgttat ctccatctaa gactttatct ttacttcctt cgctcgtctc agaaactgtg
      241 atcatatcat ttgtaagttt aagtttcgga agtccttcct gctctccgag attagccact
      301 tcattataac cgtatgcgat atcatatact gccgccgctg tcacaccttc acctgctgtg
      361 atcgcagctt tcttaacttc atgaaatctg ttcttttcat tataagcaga tccttccagt
      421 gttccgtcta cataaagttt cagcataccg ttgttttctt tcacacctac tactttatgc
      481 tctgctccgt cattgattga cttgccggaa actgctgtcg caccattcag tgtgaaggat
      541 gcttttcctt ctgctgttac tttcagttcg tactgaccat tctggcttac gagtgactta
      601 tctgttgccg ttgtgtttac agccgcatat actgtgaatc cattattgga attcagtgat
      661 ctgtccgctg ctgcaatttc tcctgcttct gcaatcgctt ctttttcaac gatcacactg
      721 tctttgtgat atacaacaga tgcagcttca gtagctttgt tgcccgacat gtctttaata
      781 tcctgcggaa ttacttttac tgtagcttcg tttgccggag cttctgctaa tacgatatga
      841 tatgttgtat cgtctgcgct cttcttaata gaagaaactt ttgcgttttc tactttaaag
      901 aggtttcctg atactttctc atcgaatttt actgtaagtt ctttcgcacc gtctgtcatg
      961 attctgtcga tcttcggcgc tgttgtgtct ttttctgctg aaacacgtaa gatacgcact
     1021 tcgttcggtt tcaagttcac tgtgtattct tgaccatact ttaatgtacc tgtctgagca
     1081 gacttatcgg aaagaagata gctgtgctct aagtagtagt ttaatgtgcc tgcaccttct
     1141 gcaacgccca ttgtacggtc gaatgtgaac ttgattgtct tatctgcatt tgcagatggg
     1201 ttacggagtg aaataattcc gtccgttccg tcgaaacatg agaatccgta tgcttctgcc
     1261 tgcgtttggt ctgacagatc tccgttactc agttttgtaa tatccggttt ccctccgatc
     1321 atcttggaat ttttgagcat atgatagttt tcttctgccc actcaaggaa ttctcctgtg
     1381 atctcgtatt ttccatctgt cataatactg tcagagtagt acaactccca aaatgccgtt
     1441 ccacgagtag acaacatgta cagatagttc tggaaatctt catctgtagc agtattaact
     1501 gtcattcctg ttccttcttt tccgtaaatc ggatcatggt tgtacagatt ctgaagcggg
     1561 aactggaact gatgattctt caggaagtca tagtaacaag cgtcacgata tgtaatctgc
     1621 ttattcatct ttgttgttcc gaaagatgca tccttctgat catgtacgca ctgaatccat
     1681 acagagttcg cccactgtaa ataccacgga ctcgggttta cataacatgt aagagagatc
     1741 cagagtttat taattccgtc ctctttttca ctctgacgaa ccgcttccat cagatcgatc
     1801 catgcttccc acagatcagt cacatgatac atctggtgat aaccgcctgt catatggtga
     1861 ttagactcac tgtataccgg aacgccgtct gctccgccta aattgttgaa atgattatac
     1921 tgagcagtat ctgcaaatcc atcccatttc cagtagttga catcccaatc ttgctgccat
     1981 ttaatcgcca tctttttaaa attctctaca tatacacggt ctgcaacgtc gatagaatgt
     2041 cctgctttac ttcctttttg tgccctctca ataatatttg ctaatgtagt gtagaagtta
     2101 tatccgcctc tcggtccaac ccatactcca aagttacttc cgaacttctt aacaagttcg
     2161 ctggacggtg tcaatccctc cgggaatttc tcattaaatg tccagaaacc ttcggtattc
     2221 tctattgctc ccgattgtgc gtgtgaccct gctcctatcg aaccatcatt atacgcattc
     2281 catccatcat ctacaacgta agaatccaac ggacgaactt ctgctttatt caactcacga
     2341 tcaatttcaa tgaacgaagc caaaatattt tcatcactga tcttcatcat gttgtcgaac
     2401 catgaattgt actgtgtacg gaactctgac ggagttgcga ttgacttgat gtactcgaag
     2461 aagtctgcct gaataacttc ctgctctgta cttcttgccg cacctgcaac tgtctgccat
     2521 gtcacatact tatcgtctgt tgtcaactga ttatcttctt tcagtctgct gaacgtctta
     2581 ccactgtaat aacgcataaa tcctgttccg tttacgatct ctgtatccgc tgccgggaac
     2641 tcacatccga agaacattcc ctgaatgtag atcggctgtc cgaggttcgc tttgaactct
     2701 tccatctgaa caacgccgcc ttgacctctc ggaattgtcc actgtgcatc ggactctgcc
     2761 actttcagag actcaaggtc gatatagtcg atctcagcat ctgccatctt gtcatccggc
     2821 acgtcgatct caaggaattt acgcatgaag tgatcgcctt cgtacattac gattacttcg
     2881 tcgattgaat actctacgtt tttgtgagtg tacggtttga aagaaaatgt tactttctta
     2941 cctgttttct ctttaccgtt gatcgttgcc gttgtatctt cgactttgac tgcctcattg
     3001 ccgtctttca gctctaaatc gcttgtctca aatgcacggt catccgtatc taccggagct
     3061 ttatctgcgt ggagattaaa ttctgctccg ccggcaaatc tttccccatt gtttgaagat
     3121 gttgctacga acttaatctg tttcgcagta catgcttctt tcaagtttac atagatcggg
     3181 ttcgtaccat tatattcaaa ttcgccttct gcgatcggct ctccccaatc ttcggattca
     3241 taatcaagtt tgttttctgc tgttgatgcg tacagcttgt accccttaat atttccgttt
     3301 gtgttttctc cttcttttct cggtgtataa gagaaagact ggaatgtcac atcttttccg
     3361 aatgtgatta ctacattatg cggatagtcc tgatcacctt gtccagtccc cccccccata
     3421 atttgaatgc cagatactct ctgttcttcc atctaacaag ttagatgccg gtccatctga
     3481 atctccgctc gcattctggt agctgtctgc cgttgctgtc cagccgtcac ggttgattgc
     3541 ctctaaggta atcgatcctt tttgctcttt cgttgtctta acaacgaatt cttcacttcc
     3601 ttcttgcgga gtgaaggttg ttccttctcc accagttctt ttgttcacaa tttcagtcgt
     3661 ggacaatttt ttgtccgctg ttgaaaatgt tcttttgatg gcatcgtttc cgattgtaac
     3721 agtattatct ttctgctcaa cggttacttt accatacgga actgcagttt cattttctgc
     3781 cgctccggca actgatgttg tggagaagac gctcgtcaca accatagctg ctgcgagcgc
     3841 tgcactgaaa aatttctttt tcataatatt ctctcctttc cccgtgaaat tgttatgttt
     3901 ttcacagtct gcatctattt tatgataaca tattccctgt catttcggta catccaatat
     3961 ccttttcgtg atatttactt aatttgtgtc aatttaatca atgttttttt aagattttat
     4021 cggtttttct taagaattct tctttttatt ttccgcaatt tattaagacg actataatat
     4081 cctgtttatg tcaggaaata aagggaatcc tgtaaataac acttaaagga gttgcattat
     4141 atatgaagaa actcatttat ccggcagcgc ttattctgct ttttattata ttgacaacga
     4201 caattctgcc tgccgacgcc gtctacggat cgaatacgga ctggttttcc cagcatgccg
     4261 ctcttgcgga aacgatccgt gacgcctgcg tggaacagca cacgcttctt cccgactgga
     4321 tcgatctcgg aagcggaagc aacggatatc agttttctta ttatggattt ttaagacctg
     4381 atattctgat cggatgtctg tttccgcaga ttcctatggt ttacatcctg atcagctata
     4441 tgcttgcact ttatctcgct tccgttcttt tatgttatat gtggctgcaa agcgaggata
     4501 tttccccgtt cttttccttc atcggaagcg ttttattttt gactgcggga tgtcttttcc
     4561 atatgcatcg tcagatcatg tttgtcaatt atctgccgtt tttgatcctt gcttttttgt
     4621 gcgtgaagaa acgtaagttt cgctttctcc ctctttgtat gcttctgatc tgcctgcaca
     4681 gcttctattt tgcaatcgcc gcatttgccg cgatcggctg gtactggctg agaacggaaa
     4741 aagagacatt ttggaaagat tcgtttttca aaaaatatat cccttccgcc cttatttccg
     4801 tctgcatggc ggcggcgctc cttctgccga caggacttgt actgatggag cacagacgaa
     4861 gcggcggcgg attttctctt ttgaaaatat tagagctttt cggaccgaat atctctatga
     4921 acggcatctt gtttaacgaa tacggactgg ggcttacatt gatctgcttt tacacgatac
     4981 ttgccggatt aaataaaaaa aatcctgcca aaaaagaatt ctttcctgac agcgttttat
     5041 ttttatcatt cggattgttc ggtatctttt cttacatttt aaacgtaacg ctctacgcca
     5101 gaccgaagat acttattccg tttctgccgc tgttcatcct gcactgcgta cgttacttaa
     5161 aatcagcgca caccgaaaca gaccgcattt catcgtatcc gctctatccg tttgccgtaa
     5221 tgcttccgct tggtctgctc tggttcagtc aagttcagtt tccgtggatt ttgatcgaac
     5281 ttggtattct gtttttattt tgcctgatac aaagacggaa acaaaacctt attttgcaaa
     5341 tgtttaaaaa tccccgcatt tttgcccggg tagaattact cgtctatctg ctcatactga
     5401 tcgccccggt cggtatgtat attacaactg ccagaaagga aaactgggta aagaaagcag
     5461 atgtttcggc aaaagaaact tacacaaaac ttgcggaaat tccgatggat ccgctctatc
     5521 attttgacag cctgaccgaa ccgcttgcaa acggaaaccg cgccccagaa gcgaaaataa
     5581 caagaagttc tatgtattct tctgtaacaa acagcgctta ctccgacttg tattatgacg
     5641 tgctgaatac accgatccga atcaataacc ggatcgcgct gcttacttcc gataatccgt
     5701 tcatgcttca tcttcttggc gttcggtata tcgagacaga aaaagttcat attcccgccg
     5761 ggtatacgcc tctgtattct tcggcaaaag acacggtggt cgcagagaat aaaaacgtgc
     5821 ttccgaatgt ttattttaca tcggatacaa tctctgaaaa agagtttgac cgtttcaatc
     5881 aaatcgaaca gttggaagcg atttcccgca aaaccataat agaaaacact cccactgata
     5941 cggacagcga cgtttacctc cccggaaagt ttatcactcc ttttgcccct aaactgtcgg
     6001 cagacggcaa acttcccgat agtttgacta tcaaaaagac tgccgataag tatgacataa
     6061 tttcaaaatg tcaacaatcg ttgacatttt atgttgaaaa tacaggtttt ggcaacattt
     6121 tgttgctttc tttccaggtg gataataaaa cgatcgatcc tgttgtcatc gacattaaca
     6181 acatacgcaa taagctttcg ggactatttg cgccttaccc aaacggaaac aatacgtttc
     6241 actatcagtt ttcagcggac agcgactcgg gtatgacaaa actaaaggtt acttttccaa
     6301 agggtcattt cactgtcagc aacgtacagt ggcatttatg taataaacat attttcgacg
     6361 acaagaacac aattacaaaa gcggtctgtg acagcagaac cgaacgcagc gccttccttt
     6421 caggcacgac tgttttttcc ggaagcatcc atgcagaaag caacggggtt ctcgcatctg
     6481 cgattccgcg gcaaaacgga cttgagcttt acatcgacgg cagacgcaca gatattatcc
     6541 gcgtaaataa agcttttgca ggagcacata tcgaaaaggg gactcataag atcgaatttc
     6601 gattttctcc tcccggaaaa caaatcggct gtatcatcag cctgatatct ctgttgtgct
     6661 atttaagtta cctgattttt acatttggag tctttacttc aagaaaaaca caaaatatta
     6721 caacggctca ccataaaaac gcgctgtaag aaattcacta taatcattat aagaaagagg
     6781 gaactgactt tgaaacgaat ttacttaagc aaagaactgc tcacttacat cattaccgga
     6841 ggaatcacaa ccggagttaa ttacatgtta tacggacttt ttctattttt tcacgttccg
     6901 tatattgcat ctaacagtat cgcatggaca ggcgcggtta tcgccgccta tgttctgaac
     6961 agaaaatggg tctttcggtc aaacagacgg attcccgaag agtttttctc ctttgctgct
     7021 ctacgattta tcacactgct cgtcgaaaac gttctgcttt ggcttgccgt cgatcaatta
     7081 tcactttctt tgatcggctc aaaattaatc gtaagcgtcg taaccgtgat cggaaactat
     7141 gcgctctgca aataccgcgt tttcaaaact gccgctcatg ggcaggatca aaatatgatc
     7201 agaaaggagc tgcttccata tgacaatcaa aaataatcct gctgacgatg tgatcagcat
     7261 tatcgttccg tgttttaatg aagagagcgt tctccccgct ttttacagcg cggtttcaga
     7321 cgccgcatcc tcgatcgaag gcgctgtctg cgaatttctt tttatagatg acggaagttc
     7381 tgaccgaacg gcagagatcc ttcagcattt tacagagaaa gacgagcgtg tccgttttct
     7441 tacattttcc agaaacttcg gaaaagaagc cgccatgtac gccggacttc aaaatgcatc
     7501 gggagactac tgtgtattta tggacgccga tctgcagcat ccgcccgctc tgcttaaaaa
     7561 catgtatcac gctgtcaaat acgaaggata cgactgctgt gcaggactta gagaggaccg
     7621 aaccggagaa aatccgatcc gcacgttctt atcccgctct ttctatcact tgatcgggaa
     7681 cgcatgtcat ctcgatatgg gagacggaaa aggtgatttc cgaatgatga acagagctat
     7741 gacggactct attttggaac tcaaagaata caaccgctat atgaaaggga tcttctcttt
     7801 cgttggattt gatacaaaat ggattccgtt tcacaacact gagcgcgccg caggaaaaac
     7861 aaaatggaat ttccgctcgc tgttccgcta tgcaatggac ggaatgttat cattttcttc
     7921 cgcccccgct tccctgcccg gcttcatcgg catgtttctc ctgatcgccg ccgttgtcac
     7981 aggacttgtt tccgttttca ccggacataa agcaaactgg atccttctgc ttgtctgcct
     8041 gatcctcgcg ttgaacggca tacagatgat cttcctgtca ctgctcggac aatatatgtc
     8101 gaaagattat atggaaagca agaaacgacc gatctatatt ataaaaaaga aaggggggtt
     8161 ttaacccctc cttttctacg acagctctaa gtttcctgta taaagctgat aatacgttcc
     8221 tttctgtgac agcaactcat catgtgttcc acgttcgata atacgtccgt gttccagcac
     8281 catgatcgca tcgctgtttc ggatcgtcga aagtctgtgg gcgattacaa atactgtacg
     8341 ccctttcata aggttatcca tacctttttg aacgatactt tctgttcttg tgtcaataga
     8401 cgatgtcgct tcatccagga tcagtaccgg cggatcggca atggcagctc ttgcgatcgc
     8461 aagaagctga cgctgtcctt gagacagttc ttctccgtct ccgctcaagc gcgtctcata
     8521 acctttcgga agcattctga tgaatccgtc tgcatgagcc agctctactg ccttataaac
     8581 ttcctcatct gtcgcatcca gtttcccgta acgaatgttt tccataatcg ttcctgtaaa
     8641 caaatgtgta tcttgaagaa cgataccaag cgatcttcgc agatcagcct ttttaatctt
     8701 gtttatatta atatcatcat aacggatctt tccgtcctga acatcataga aacgattgat
     8761 caggtttgtg atcgttgtct ttcccgctcc cgtcgaaccg acaaaagcaa gtttttgtcc
     8821 cggcttcgcg tacagagtca gatcatgaag caccatgtgg tcttccgtat atccgaacgt
     8881 catatcataa aatctcacgt cgcctttcag ctccgtatat gtcactgtgc cgtctgcctg
     8941 atgcggatgt ttccatgccc acaagcctgt tctttcttta cactccgtaa gatttccctg
     9001 tgcatccttc tttgcgttta caagagtaac atatccttcg tctacttccg gctcttcatc
     9061 catcatatta aaaatacgtt ccgcacctgc aagagccatg atgattgagt taaactgctg
     9121 ggcaatctgc ataaacggct gagtaaagct cttcgtaaac tgaagataag acgccatcac
     9181 gccaagcgta atgtttccga tgccgagtac cgaaagcgcg ccgcccagta cagcagtcaa
     9241 aacgaactgt aagtttccca tatttccgat aaccggaccc atgatacttg caaacgtatg
     9301 tgcctttgat gaactgacga acagactttc attcagctga tcgaattctt cttccgaaat
     9361 ccgttcatgg ttaaatactt tcacgacccg ctgtcccgtc atacgctctt ccacaaatcc
     9421 cgtgatattt gccagatcaa gctgctgccg tacaaagtat tttccgctgt ttcctccgac
     9481 cattctcgca acagccgtca tcaccccgat catcacaacg gcgagaactg tcaggatcgg
     9541 acttaaaaca agcatcgcaa taaatgtgac tacgatcgta agagccgaca taagcacctg
     9601 cgggatcgac tgattgatca tctgacgaag cgtatccgta tcgtttgtat aaaggctcat
     9661 gatcgaaccg tttgtattcc ggtcaaaata acggatcggc agtttctgca tatgttcaaa
     9721 catctcgtcg cggacacgtt tcagcacgcc ctgcccgatc acgaccataa gtctcgtgta
     9781 aatgaaggag gcaattacgc cgctcaagaa aatacatgcc aatataccaa gcgcctgata
     9841 cagttctgtg taatccggac ttttttgccc gatcagcggg ataatataat catccagcag
     9901 aaacttcagc gacaatgaga ccgagatcgt cgcaatcgaa ctgacaagaa tacagatcag
     9961 tacgataata aactgtactt tatatgtttt cgtcacatat ccgagtagac gttttgctgt
    10021 ttttaatgta tttttattca acgacggttt tgtcggtttc ttttctttac tcactgtcct
    10081 ttcctccttt catctgtgat tcatatactt ccctgtaaat cgcattcgtt ttcagcaatt
    10141 cctccgatgt tccgattccg ctgatctctc ctttgtccat tacgataata acatccgcat
    10201 cctctaccga agaaactctc tgcgcaatga tgatccgtgt cgtatccgga atctcttctc
    10261 tgaaagcctg acgaatcagc gcgtctgttt tcgtatctac cgcgcttgtc gagtcgtcca
    10321 gaataaggat cttcggcttt ttcaaaagcg cccttgcaat acaaagtctc tgcttctgac
    10381 cgcctgacac attatttccg ccttctacga tcatcgtctc atattgattc ggaaactctc
    10441 tgataaatcc gtccgcctgc gccagcttac atacgcgttc tacttccgca tcacttgcag
    10501 tttcatcacc ccaacggatg ttctcataga tacttcctgt aaaaagtaca tttttctgaa
    10561 gaacgacaga cacctgatcc cgcaaagctt tcaaattata atcccgcacg tctgttccgc
    10621 cgactttcac tgttcccgac gtcacatcat acagtctcgg gatcaactgc acgagcgttg
    10681 acttagcgct tcccgtaccg ccgatgattc cgacgatctg accgctcttt atatgaaggt
    10741 ttacatcttt caacgaaaga ttgcctccct ctcccgcata actgaagttc acatgttcaa
    10801 attcgatatc tccgtttgta acttccgtaa ctgcatccgc tttgtcttcc atttccggta
    10861 cttcatccaa aacttcgttg atacgttccg tagacgcttc cgcgatcatg atcataacga
    10921 atacaaacga caccatcata agagacgtca gtatctgaag tgcatagacg atcacacttg
    10981 tcagctctcc tgtaagcata tctccgctta tgatactttc gccgccgatc agcaccattg
    11041 caagaacgac tatatacata gtaaactgca tcgtcggaga gttccaggca acgattttct
    11101 ctgcttttgt aaaaagccgg aatacaatac ccgagatctt tccgaatttt tcaacttcat
    11161 ggtcttcccg aacgtacgct ttcacgacgc gggctgcgtt gacgttttcc tgaacgacat
    11221 tattcaattc gtcatattcg tcaaacacct tgataaaatg cgggtgcgct ttctttgcga
    11281 taaagatcaa aagtcctccc aaaaccggaa tcgtgatcaa catcataaat gcgatcgcag
    11341 gactgatcgt caccgtcatg atcagggaca tcacgatcat gatcggcgct cttgcgagca
    11401 gacggatcgt aaacatatac gccatctgta cattcgtaat gtcggtcgtc agtcttgtca
    11461 caagacccgg cgttgaaaaa tgatcgatat taccgaatga aaaatcttgt attttataaa
    11521 aaatatcatg tcttaaattt ttagcatatc ccgaagacgc taccgccgca agctgacctg
    11581 ccttcgctcc gaaaaaaagc gccacgaccg caagcagtac gagcaaagcg cccagcttta
    11641 tgatatatgc catatctccg ttcatgatcc ccacatcaat aatcttcgcc atcaaaagcg
    11701 gaatgagaac ttccacaaac acttccgcgg ctacaagaac aggtgtcaga aacgattgtt
    11761 tcttatactc gcgcactgag cgcagtaatt ttttatccat cttttctatt agctcctctc
    11821 tctactattg atttttgttt acatgctgct ttctttcccc cgaactgttt ccctcccggc
    11881 acagtttcct ttgatcggct ttgatcacac tgcgccggct gccgccgtct tttgtttttc
    11941 ggtacagcat ttttcatctt caaacaaatt ggcggaaatc agttccagcg tttctataca
    12001 aaccgccatt ttttcatcag gtatcccctg acggatgatt ttttccgtac gatcaatgtt
    12061 catcaataca tgttctctga ctttttccgc tttctctgtc ggcacgatct ttttcagccg
    12121 cgcatcccgg tcttctgctt tccgcaaaat aaatccgttt ttttctaaaa gctgtaagat
    12181 tcccgtcgct gtggatctcc ggatctgaaa ttccgcctca acatccttct ggtaaatatc
    12241 cctgctcatt gactgaagca atatgtaatg aagtacatgc ctttgcatat ttgtcaaaga
    12301 atcctcttcc tcatgacaat tcattctccg cttcagttga tgcgagatcc gattgatgat
    12361 ctttcctacg tcacaccgct cttccataat ccatcctctt ctctttctga tttccgtatc
    12421 cgcatttttc gtattgactt ttcctttttt acgaagtcgt ttgacctctg tattatgcac
    12481 aaaattataa tagttcgggt gctaacaata tagtatatgt agcaaaattt ttaatgtcaa
    12541 tccttttttc tgtattattt ttcaattttc tgtattattt tataatttat actattttga
    12601 cgaatttttt actatttcgc acaataaagt atggcatttt cacgaataga attgtataat
    12661 aaaggggagt aaattataag ggaggactca agcatgaaac aaaataatat gttagccatg
    12721 atcttggccg gtggacgcgg ttcccgtctt catgacctga cccagaaagt cgccaaaccg
    12781 gctgtttcat acggcggaaa atatcggatt attgattttc cactcagcaa ctgtgccaac
    12841 agcggaatcg acgttgtagg cgtattgact cagtacgaat ccattcttct caacagctat
    12901 gtagctgccg gaagacgttg gggattggac gcgaaagaca gcggcgtcta tgtactcccg
    12961 ccccgtgaaa aatcagatgc caatcttgat gtttaccgtg gaactgccga tgcgatttca
    13021 caaaacatag actttatcga cacgtattct ccggaatatc ttcttattct ctccggcgac
    13081 catatttaca aaatgaacta tgcaaggatg ctcgcattcc acaaagagag taaagccgac
    13141 gcgacaatcg cagtcattga agttccgatg aaagaagcga gccgattcgg tatcatgaac
    13201 accgatgaaa caggacgcat cgtagaattt gaagagaagc cggaacaccc gaagagcaat
    13261 cttgcttcca tgggtatcta tatcttcaac tggaaactgc ttcgcaaaac gcttcttgcc
    13321 gatatgaaga acgcggattc cagccacgat ttcggaaaag acattatccc ggcgcttctc
    13381 aatgacgaga agaagctctg cgcttacaaa tataagggat actggaaaga cgtcggaacg
    13441 atcgactccc tctgggaagc aaacatggat cttcttgaca aaaacaatgc acttgacctg
    13501 aacgatccgg catggaagat ttacacagag gacgtaacgg ctcttccgca gtatatcggc
    13561 gccgacgctg ttgttgaccg cgcttttatc acacaaggct gcgttgtcaa cggtaaagtt
    13621 aaaaattctg tcctgtttac aggagcaaaa gttgccgaag gagcacaggt tatagacagt
    13681 gttcttatgc cgggggcaga agttgccgaa ggagctgtcg ttacacgcac gctcgttgca
    13741 gacggtgtca agatcggtaa aaacgccgtt gtcggaagcg ctgacagcaa gaatatcgaa
    13801 cttgtggcaa aacgtgtaaa gggggatgaa taatatgaac agagcttttg gaattataaa
    13861 ttttgccgga aatcatatta aagtagacgg tcttcaggat tttcgtcctg tcggcgcttt
    13921 ctctttcctc ggacgttacc gcatcattga ttttccgatt tctaatatga gcaacagcgg
    13981 aattgacaat atcgaagtat ttatcagaag aaaacctcgc tcccttacag agcacctcgg
    14041 aacaggccgc cattacaaca tcaactcaaa acgcggcagt ctgaatatca tgtatacaga
    14101 acacggtcag atcggcgaca tttacagtac cgacattacc gcttatatgg aaaacataga
    14161 gtgcctcgag gagatgcctc atgaatacgt tgtcatagct ccgagttata tggtttacaa
    14221 agctgattac agcgaatttc tcgacgctca tatcgaatcc ggcgcggaca tctctatgat
    14281 gtatcactcg gtcgataacg ccaaagacgc ttttttgaac tgtaacgctt tgaatatcaa
    14341 caaacaaaaa ggagttcttg nnnnnnnnnn tgcaatcgaa ccgaatcacg gaaatgcgaa
    14401 aaaccgcaac atttccatgg atacatatgt tatgaaaaaa gatctcttca tcgaacttgt
    14461 tcgcaaagca agcaaaagat catctatgta cacgtttgcc gacattcttg atgaagaatc
    14521 cgaaaatctt gatattcgtg cggtatccca ccgtgggttc tttgcgatca ttaatgattt
    14581 taacgcatat tatcaggcaa atatgtctct tgtagacctg aataatgccg gaagtctttt
    14641 cgacaaagac tggccgatct acacgcacac gaacgattcc tgtccgactc agtattttga
    14701 aggaagcaag gtgacttctt ctgttgtttc caatggatgc ctgatcgaag gaacagttga
    14761 aaattccatc atcggaagag gatgtgttat caaatccggc gctattgtaa agaattgtat
    14821 catatctgcc gatgttgtta tcggaaaagg cgttcatgcg gaaaatctcg tcgtagataa
    14881 acatgcgcgt ctcatccgcg gaaaacgtat catcgccgct cctgaaaaac cgggatatgt
    14941 aaaacgaggc gataaacttt aagttttaaa ataaatgaat gtaaaccttc aagattttcc
    15001 ctcttgatta ttcagggagt acatggtcga acatgcgtca tgtactccct gccccttttt
    15061 ctggattttc tccatttttt cctgtataat gatctgtatc tgaaactgct tacatacacc
    15121 cgaaaaggag tacgaactat aatgttaaaa tcttatatcg ccttcgatct tgaaacaaca
    15181 gggttagatc cgatgaacaa tgaaattata gaaataggcg ctttaaaagt cagagacgga
    15241 aaagtcagcg aacgctttat ggaatttatc aaaccgcagg aactcatttc gcccgccatc
    15301 acaggcctaa ccggtatatc aaacgatatg gtcgcatctg ctcgtcccgc ccgttctgtt
    15361 atcacagact ttattgattt ttgcgaagaa gatattctca taggtcacaa catcatcttt
    15421 gattacagtt ttacaaagtg tacggctgcc cgtgaaggac ttccgtttga aaaaatgggg
    15481 atcgatactt taaaaatcgc caaaaaagtg catcccgaac tagaatccaa aagccttagc
    15541 gctttatgcg aacattataa aatagaaaat aaggcagccc accgcgctta ttttgacgcg
    15601 cttgcaactg ccaaattata tcagacgctc tcccattatt ttgaacctga caatccaaag
    15661 acgttcaaac cgtcgcagct tacatataag atcaggaagc ctcagcctgc cacaccaaaa
    15721 cagcttgctc ttcttgaacg tctgacaaaa aataaaaaaa cgttttctca taatacagaa
    15781 gcgttctctt gcaatgcaga agcgctctct tataatccgg aaacgctttc acgaagcgat
    15841 gcttccaggc tgatcgacag attactgaaa gggaattgat tcttacattt tccacatata
    15901 atcttcaact atttcaaacg gcgcaggtcc gattttcagc acagccgaat gaaattcctt
    15961 ttgtaagaaa tcttttcctt tctcgtcaac ccacattttc tttaattcaa ggaattctac
    16021 atacccgata tagtatttca gatagttggc aggcgaaccg aggatcagct tatagatttc
    16081 gccgactgca tctttatctg taatcccgta gtttttaaaa aatgcttccg tatcttcgac
    16141 gctccacccg tcataatgga ttccgatatc cgcaagggca taaagcgcca gtatgataga
    16201 ggcgtttttc tgtaaaaggg tcgcctgctc tttggaaagc cccatcagat aataactgcc
    16261 catttccgca taagtcgccc atccttccac atatccgccg aaatcaagaa gacttctgat
    16321 tggatcagga tgcgtacttt cataataaat cgtctgataa agatgtcccg gatatccttc
    16381 atgagcaagt gtcgtaaaaa gcgtcatatc gtttcccatg cgcgccctgt taatatagat
    16441 cacgttttgc tgtgaattat cgatcgcggg tatcatgtaa aacgcaggac ttagatgttc
    16501 ctccattgcc tccggaacat actttacttc cagctttgtc tgaggcggtt ccggaaacgc
    16561 agttttcgat ccttctttta actgttccaa tatctgctct gcctttttat ctgtgatcac
    16621 cgccgcagct tccttagctt cctttgttgt cagaccgagc acttgctcca tcgccgtaag
    16681 atcttcgttt atctgtctgc gtgtcaaatc ttttagttcc tctaccgaac gtcttgaacc
    16741 ggttgactgc cgggcaagaa gttcgtaata ttccttcccc ttcggaaaat atacaagacc
    16801 tttttcattt ttacccgatc ctttgagctg ttccagttcc tccgtcatac gctcatacgc
    16861 aggaaacaca taatcggata ttgcaagggc attttcctgt atgtagtcac ttttttcttt
    16921 ctctgtcaat gtatttaatt ccatcactcg atctgcaaac gtcgaataca agtaatttcc
    16981 gttccccatt tccaaaaagg cgcggcactg cccgatcact gtttccaccg cctgatccgg
    17041 catgaacaaa cccatctctg cctttttctt ttcaaatatc atgatctctt caaaatattc
    17101 ttccgtctgt cttacaagcg ccagatatac ttccacatct tccttatcat aaaaacgata
    17161 ttctgataaa actaccggaa gctgtgtctg cactccgctt acaagcccga gcggttcttc
    17221 atacattgta taatccgcgc tctttttcat cagctttaac cgataattta aaatgtcata
    17281 tgtaatctta ttttgtacgt taagatcatc atagttgaat ccaagaagag cctgacgcat
    17341 attttccgcc gccgccttga ctgcttccgg ctctctttca aactgaccga atgtagcgtc
    17401 agttgcttca atcccatatt tttccggcgc tttaagcgta taatgcagac tcactgtatt
    17461 tgccgacact tctttgcaga atagttccgc cgtgtattct tcaaatcttg catcttcact
    17521 ttgccttttt cctgcgcttt tcgcaccttt tgctccctgc ttactctcca ttgagcatcc
    17581 gcccactgac gccacagaaa atacaagtat tataacgagc aaaagattca aaattttgaa
    17641 atatattcgt ttcttttgat tattatttac tttctctttg ccgttacatt tccatatcgg
    17701 cataataacc cccgaatctt cttttactgc atcctatgtt atatccggcc ttaatataca
    17761 ttgctttttc ttattttagg aatggatctt cctgccgtac atataatcaa ataatccgta
    17821 cattctgtaa aggaaggttt attatgcagc ttctcgtcta tatttcaaac tatattgttc
    17881 cgttccttat actaagcatc atcgtttacg gtctgtccgc aaaggtcaat gtttacgaaa
    17941 catttatcaa aggagcaaaa agcggttttt ttacagtcgt tcggatcatg ccgacgctta
    18001 tcggacttat ggcggccgtc ggaatactcc gcgcttccgg gttccttgat ttcatttccg
    18061 gtctgcttga aaactttacc ggaaggatcg gttttcccgg cgaactcgtt ccgcttacta
    18121 tcgtaaaaat gttctcttcg tctgcagcga caggactcct cctcgatatt ttcaaacaat
    18181 tcggcacaga ctcccgtctt ggtatgatcg catcgatctc gctgtcttgt acagagacaa
    18241 tcttctatac gatgagtgtt tactttatga ctgccggaat caaaaaatcc cgctacacgc
    18301 tcgccggtgc gcttatcgct acattcgcgg gacttgccgc aagcgtcctc ctcgcataac
    18361 ctgagcagca tctaagtgcc ggttttattt aaggctttga atatggttcg gtttaagact
    18421 tgaacgtcgg tccgatttaa gatttgagta tggctcggct taagacttga acgtcggttc
    18481 ggtatttgaa catcggtttt tgtttaggat tgtagaccga ggcgctttat gctaatatat
    18541 tactataatg tgatcatgga ggtgttttta tggcaagtgt gacattagaa cacttatcga
    18601 aaatttactc aaacggtttc gtctccgtca atgatgtcag tcttcgtgtt gacaacggcg
    18661 aattcattac tcttcacggt cccagcggat gcggaaaatc tacgatcctc cgcatgatcg
    18721 gcggattaga ggatatcact tccggtaaga tttatctcag tgatgaatta ttaaacgata
    18781 ttttgccgcg tgacagacgg cttgcgatgg cgttccaaaa ttatacgctt tacaagcatt
    18841 ttaacgccta cgacaacatg gcgctcggtc tgcgtctgag ggattttccc cgcaacatta
    18901 tcgacagccg tgtaaacgat gccgccaaac ttctcgggat ttcaggagtt ctggggcaga
    18961 agatcaaggc gctttctgaa agagacaagc aaaaagtcgc tct
//
LOCUS       DBMDXF010000011        18600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_919_length_18600_cov_1.863506, whole genome shotgun sequence.
ACCESSION   DBMDXF010000011 DBMDXF010000000
VERSION     DBMDXF010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 18600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18600
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_18600_cov_1.863506"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="ACZPD4_00955"
     CDS             <1..367
                     /locus_tag="ACZPD4_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR1377680.1"
                     /translation="FLTICNVFSLIFVFSFTSSHNKITLQEGLSAWGFGVLFSLVFFA
                     VVNFAFFLLGRKLGLIVYIFIAMLFGGIYGAVSSLKGMEFLMAGDKTMIFIIGIPIGI
                     LFVVISFFLSLACYKKRYS"
     gene            complement(392..1702)
                     /locus_tag="ACZPD4_00960"
     CDS             complement(392..1702)
                     /locus_tag="ACZPD4_00960"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIR1377681.1"
                     /translation="MNRKIYNEYVTILESELVPALGCTEPIALAYAAAKAKEVLGKMP
                     DHITMRCSGNIIKNVKGVKVPNSGGMKGVEAAAVLGITGGDPSQALEVLEHVTDREID
                     EAEKLLKAGFCDCVLKDDVANLYIEAYAVCKKTEKSEALVVIEDEHTNITHIEKDGQV
                     LFHKEKKEYCQEREKTPDKSLLNLEDIITFANEVQITDVEKVLGRQIKYNTRIAEEGL
                     RNPWGAQVGRVVLEEFGEDVKWRAVAKASAGSDARMSGCALPVIINSGSGNQGMTCSL
                     PVIEFGKELKKSKEEIYRALCVSNLVALNQKKYIGSLSAYCGAVCAAAGAGAGITYLC
                     GGTLEQIENTVVNTIADAGGIVCDGAKPSCAAKISTALQAAILSHKMAMRGLTFARGE
                     GLVMDCPEDTIKAVGYVGRAGMKQTDVEILNLMIGKTKLEDIDK"
     gene            complement(1883..2062)
                     /locus_tag="ACZPD4_00965"
     CDS             complement(1883..2062)
                     /locus_tag="ACZPD4_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR1377682.1"
                     /translation="MAADILIISLIASYCIFLIYKARKNAKEGKAPGCAGCGGNCGSC
                     SGCATSAGKTENKKM"
     gene            complement(2092..4275)
                     /gene="feoB"
                     /locus_tag="ACZPD4_00970"
     CDS             complement(2092..4275)
                     /gene="feoB"
                     /locus_tag="ACZPD4_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1377683.1"
                     /translation="MEIRAALAGNPNCGKTTMFNALTGANQYVGNWPGVTVEKKEGKV
                     RTKESKEEKIKVTDLPGVYSLSPYTLEEVVSRDYILNEDPDVIINLVDATNIERNLYL
                     TTQLTETGVPVVIALNMADLIRKRGMKIDKKRLSELLNCPVIETSALKKTGLKELIEE
                     TVCTVKSGEKSIPKAIFSKELEAAIRAVEELLPTWIDERKKRWYAVKLLENDRKVLEN
                     LKMSGESLKAIEKMRKAMEEKHDDDMESIVTDERYQYIQKVVSDTVQKGREKLTVSDK
                     IDRIVTNRFLGLPIFVFVMWVVYYVSVTTVGTGMTDWTNDVFVVSIQDAVSGFLGNIG
                     AGSLITGLVVDGIIGGLGAVLGFVPQMAVLFLLLSFLEDCGYMVRIAFVMDRVFRHFG
                     LSGKSFIPLLISSGCGIPGIMASRTIEQDNDRRLTIMTATFIPCGAKLPVIALMGGVI
                     AGEAAGYAESSFIAPLMYFIGIAAVLISAIILKKTKMFSGKPAPFVMELPQYHMPQAK
                     TVLLHVWERLKGFMIKAGTILFLACIVMWFLGGFGFSDGKFGIVQDSANSLLAMIGGA
                     LAVLFKPLGFGEWQPVAAALSGFTAKEAIVTTMGVLANVSGDVEDTVNVAGKVAAWFP
                     SAMAAFSFLLFNLLDSPCLAAIATMAQQMQSRKWFWFAVVFQNVFAYVVSLSVYQIGT
                     FVLGGAFTVGTAVGIAIAVILLILLFRPDPYKNKKIYSKRSVQAA"
     gene            complement(4292..4522)
                     /locus_tag="ACZPD4_00975"
     CDS             complement(4292..4522)
                     /locus_tag="ACZPD4_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1377684.1"
                     /translation="MERTLGSAKVGSTVTVIKIEGDGAYKRRIMDMGITKGSRIYIRK
                     VAPLGDPVEVTVRGYELSVRKEDAKCVLVETV"
     gene            complement(4576..4785)
                     /locus_tag="ACZPD4_00980"
     CDS             complement(4576..4785)
                     /locus_tag="ACZPD4_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1377685.1"
                     /translation="MPLTMAEIGKVSVINRVNGNEETRRFLGNLGFVAGTEVTVLSEM
                     GGNVIVSIKNSRIAVNAEMARHIMI"
     gene            5047..5409
                     /locus_tag="ACZPD4_00985"
     CDS             5047..5409
                     /locus_tag="ACZPD4_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1377686.1"
                     /translation="MIINESAENYLETILVLSGQLPVVRSVDIAAELDFKKPSVSVAM
                     KKLRESGHITVSPEGYITLTPSGKEIAERIYERHTLLTSWLEYLGVDSKTAADDACRI
                     EHVISAESFDAIKKHIKR"
     gene            complement(5439..6389)
                     /locus_tag="ACZPD4_00990"
     CDS             complement(5439..6389)
                     /locus_tag="ACZPD4_00990"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1377687.1"
                     /translation="MVNIQKAAVIGCGFVGSTIAYTLMQKGLFSEMVLLDANKAKAEG
                     EAMDISHGLPFTHAMDIYAGEYEDIADASVVIITAGANQKPGETRLDLVQKNAAIMRS
                     IIKEIKRVNCEGILLIVSNPVDILTEVALRESGFPKERVIGSGTVLDTARLKYIISEK
                     LDVDSRNVHAFIVGEHGDSELAAWSCANIYGIKLQDFAKMRGYEHFAQDMEEVYKGVR
                     DSAYEIIERKGATYYGIGMAAEKIVEAIVRNSHTVAPISVSLDGKYGLEGLCLSIPTI
                     VGRGGAEQVLEIDFSEEEMKKLRKSAEELGAVLDQVNIEK"
     gene            complement(6629..8158)
                     /locus_tag="ACZPD4_00995"
     CDS             complement(6629..8158)
                     /locus_tag="ACZPD4_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR1377688.1"
                     /translation="MGIKIILLIIFFAVMAAVGIYSRRHVTSVDGFVLGGRSVGPWLT
                     AFAYGTSYFSAVVFVGYAGQFGWKYGIASTWIGIGNALIGSLLAWVVLGRRTKVMTQH
                     LGSRTMPDFFGERYKSKSLKITASVIVFVFLIPYTASVYNGLSRLFAMAFNIPYTWCV
                     VTMAAFTALYVILGGYMATAINDFIQGIIMLIGIVAVIAAVLNGQGGFMNAIAEMAKI
                     PSDVPLTQGQPGAFTSFFGPDPVNLLGVVLLTSLGTWGLPQMIGKFYAIKDEKSISTG
                     TIISTIFAIIISGGCYFLGGFGRLFDSPEIYDKAGGAVVYDSIIPHMLSMLPDLLIGV
                     VVVLVLSASMSTLSSLVLTSSSTLTLDLLKDNIMKKMSEKKQIVTMQVLIVFFIVVSV
                     VIALDPPTFIAQLMGISWGALAGAFLAPFLYGLYWKGVTRAGVWAGFITGVGLTVANM
                     FFGFIKSPINAGAMAMIAGLLIVPFISLITPKLKKSEIEDVFICYDEKVTITKKHSLE
                     D"
     gene            complement(8331..8807)
                     /locus_tag="ACZPD4_01000"
     CDS             complement(8331..8807)
                     /locus_tag="ACZPD4_01000"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1377689.1"
                     /translation="MLDDKYVTMEIEKVKHIALVAHDGKKRELVDWCEKNKEILKGHF
                     LCGTGTTARLIAERTGLPVKGYCSGPLGGDQQIGAKIVEGQIDFMIFLWDPLEAQPHD
                     PDVKALLRIAVVYDIPIANNLATADFMLHSKYMNESYSRQIENFDKKVQNRVEEMK"
     gene            complement(8868..10445)
                     /locus_tag="ACZPD4_01005"
     CDS             complement(8868..10445)
                     /locus_tag="ACZPD4_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HIR1377690.1"
                     /translation="MKALIYLIKRTAANNLKKAVRKPTTLLALIFGAAYGIFLIVMLG
                     TLAVTVRIDSVQGLVILVTVWTIYITVGNFMSYASRKGILFRPAHSQFVFPAPISPKL
                     VLIQGAWMNYLMSFFIWIVLAIGGLTVFHVEWWKMLFFFLIGCGVECAVEQSVMIILY
                     TNDKLPQKLIKGICFGMKVFLIAFTLMIVLYFKEKGLSVESALSFINWPVLQMIPVVG
                     WQIAVYRLVLLGPTTLNVICTVIYSVFTVFIVAAAFRMKCDGGYYEEAAKFADDYAEL
                     KKRQKSGEFVTNTGTKKRRFRRVESKITAKGARAIFYRQFLEYKKEKYFIFTKMTVLS
                     AVVAFIFAYSLQKSVSDSSMAGFILLGVVAYVALIMSGYTGKWESELKNPYIFLIPDS
                     PLKKMWYATLMEHVKAFADGCIICIPIGIFWHVPVIEIIYCILIYTVLQADRLYTMVI
                     VQSLLGEVFGKTGQSLLRMFIQMALLGVGAGVGVLAAVLISQTLIFPIVLIYGLMITV
                     AMGAIALLRFDTMEQFV"
     gene            complement(10442..11140)
                     /locus_tag="ACZPD4_01010"
     CDS             complement(10442..11140)
                     /locus_tag="ACZPD4_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377691.1"
                     /translation="MLEIKELTKYYGKNLAVDQVTFTVPNGQVGVLLGPNGSGKSTII
                     KSIAGLLRYQGEIRIKGIPARQIPAKRKFAYVPEIPNMFDALTVREHIEYVRMAYDSE
                     ITDEEIEKILKAFEMDDKQDKLGNELSKGMMQKVSICCALAVKPEVILLDEPMVGLDP
                     AAIKMLKDVVLKLRDNGATVLISTHMLEMVENLWDVMIVMEQGHIAGSYTKADAQGKE
                     LDELFFEMTGGEKA"
     gene            complement(11184..12044)
                     /locus_tag="ACZPD4_01015"
     CDS             complement(11184..12044)
                     /locus_tag="ACZPD4_01015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1377692.1"
                     /translation="MKKIGFIGVGIMGKSMVRNLMKAGYELHIYARTKAKVEDVIGEG
                     AIFHETISECVKDCEAVITIVGFPQDVEEVYFDSGNILDSAKKGAYLIDMTTTSPQIA
                     EKLYEEGTKRGFHVLDAPVTGGDTGAREGTLSILVGGDKSDYTNCHELFEAMGTNINY
                     EGSAGCGQHCKLANQIMIAGALSGVCEALTYAKEKGLDLNVFSKSVATGAAGSRQFDL
                     FGPKIIEKDYAPGFFMKHFIKDMKLALIEANRSGLNLEVLSQVLAIYEELEADGNGDL
                     GTQALIKYYE"
     gene            complement(12129..12794)
                     /locus_tag="ACZPD4_01020"
     CDS             complement(12129..12794)
                     /locus_tag="ACZPD4_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR1377693.1"
                     /translation="MNALTVKELYTLEETIAKDLFDKVTYPWEVLPNIGSFIKELGNT
                     LSSDEYEKRGEDVWIAKSAKVAPTACINGPAIIGKEAEVRHCAFIRGNAIVGEGAVVG
                     NSTELKNVVLFNKVQVPHYNYVGDSVLGYKSHMGAGSITSNVKSDKKLVVVKTSEGNI
                     ETGMKKFGAMLGDEVEVGCGSVLNPGTVVGSHSNIYPLSSVRGFVPANSIYKNKSEVV
                     EKY"
     gene            complement(12818..14860)
                     /locus_tag="ACZPD4_01025"
     CDS             complement(12818..14860)
                     /locus_tag="ACZPD4_01025"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1377694.1"
                     /translation="MKKQLYIAEKPSVAQEFAKALKLKTRRADGYLESDEAVITWCVG
                     HLVTMSYPEEYDPALKKWSLQTLPFIPSEFKYEVIPSVAKQFQIVSSLLNREDVDTIY
                     VCTDSGREGEYIYRLVEQKAQVSGKKRLRVWIDSQTEEEILRGIREAKDLSEYDNLSA
                     SAYLRAKEDYLMGINFSRLLTLKYGNSISNYLNTKYSVISVGRVMTCVLGMVVRRERE
                     IREFVKTPFYRVISTIDASGHSFEGEWRAVKGSRYFESFDLYKENGFKTEEKAKELIA
                     YLLEEKPIQCIVESVEKKKEKKNPPLLYNLAELQNDCSKRFKISPDEALRVVQELYEK
                     KLVTYPRTDARVLSTAVAKEISRNLNGLSKYPPAQPYLQDILSFGSHKNLAKTRYVND
                     KQITDHYAIIPTGQGLGALNSLSATSHKVYDLIVRRFLSIFYPPAVYQKVAIISKIKE
                     ESFFSNFKVLAEEGYLKVAGVPQGKRSVNKGKSRTDANEKEEDAEQTADQDLDTALFE
                     VIKTLKKGSVLPVGALDIKEGETSPPKRYNSGSIILAMENAGQLIEDEDLRAQIKGSG
                     IGTSATRAEILKKLIHIKYLALNKKTQIITPTLQGEMVFDVVDHSIKSLLNPELTASW
                     EKGLTYVAEGSITPDEYMMKLDRFITNRTVGVKGLNNQYQLRACYDKAAGYYKKSR"
     gene            complement(14911..17049)
                     /gene="ppk1"
                     /locus_tag="ACZPD4_01030"
     CDS             complement(14911..17049)
                     /gene="ppk1"
                     /locus_tag="ACZPD4_01030"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR1377695.1"
                     /translation="MGKTETLEYTQNRELSWLRFNQRVLEEAQDESVPLMERMKFVAI
                     FTSNLDEFFMIRVGSLYDMAVADSKKQDIRSGMTPKQQLEEIYKAVAPLYKERDKTYA
                     EIKKQLQPYGVCGLDFKELEQQEKKYVKKYFKEQVLPVLSPQIVDANHPFPHLLNKDI
                     YVVATLKMENNVMLGIVPVPQYISDVLYLPGHDVRYIRMEKVIMEYLELVFEQYQVSD
                     KNYICVTRNADIAPDDEALADNEDFRYIMKETLHKRRKMAVVRLETAAPLGKEMEKYF
                     CDRFKITPECIFRTKMPMKLGFIFAIADKIPESMRKALIDEPFTPQPAAALAEGISVM
                     EQVKRKDVLLSYPYESMDPFLQLIKEAAFHPDVMTIKITIYRLAKKARLVEYLCAAAE
                     NGKEVTVLIELRARFDEQNNIDWSERLEEAGCRVLYGFDGYKVHSKLCLITYRNRNEI
                     RYITQVGTGNYNEKTAKMYTDYSLMTSHQGIGEDAAVFFKNMSIGNLDGEYHHLIVSP
                     FSLKRKVLRHMDEEIQKGADGRIIMKMNSVTDMDFIRKVQEASAAGVKVDLIVRGICC
                     ILPGVKENTENLRVTSIVGRYLEHPRVFVFGTGETAKVYIGSADMMTRNTEKRVEVAC
                     PIYDDAIKRRLIHDLKVMLADNVKARIMTSDGTYKKKERKGEAVNAQAAFMKAALNAR
                     PPKTLPKAKKEKKSIFQKVFSAFGKRNRSC"
     gene            complement(17131..17994)
                     /locus_tag="ACZPD4_01035"
     CDS             complement(17131..17994)
                     /locus_tag="ACZPD4_01035"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1377696.1"
                     /translation="MTIKKATLVLEGGATRGIFTSGALDYLMERDLYFSDVIGVSAGS
                     CNAVDYVSRQPGRTRDCMIPTDKEGKYYYGIRDFVKEKSLMNMDLIFDKYPKELLPFD
                     FETYFNSEINCQIVTTNCLTGKAEYMTEDSDNDRLMKLCRASSSMPLLTPIVNIDNVP
                     YLDGGLADSVPIRRAQQMENEKIVVILTKNQGYRKSVLSPTMQRVYKRAYKSYPNLIR
                     TIFRRSFEYNKTMNYLDQLEKRGEIFILRPQVKPVSRLERNKETLHAFYEHGYKYTER
                     KFDDLMEYLEK"
     gene            complement(18218..18553)
                     /locus_tag="ACZPD4_01040"
     CDS             complement(18218..18553)
                     /locus_tag="ACZPD4_01040"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1377697.1"
                     /translation="MKLFGKKKETKSCCCGENCTSETMQNTENKKQEQGIKILGSGCV
                     KCMELEKATRAAVSELRLDYEIEHVTDFAEIAGYGVMTTPALVFNGEVISYGKVLTVE
                     EVKALLSKK"
BASE COUNT         4794 a         4869 c         3043 g         5894 t
ORIGIN      
        1 ctttcttaca atatgcaacg tattctccct gatttttgtt ttctcgttca ccagcagcca
       61 caacaaaatc actttgcagg aagggctgtc tgcatggggg ttcggagttc ttttctccct
      121 tgtctttttt gcagtcgtta attttgcatt ctttctgctc ggaaggaaac ttggtcttat
      181 cgtttatatc tttatcgcca tgcttttcgg aggaatatac ggagccgttt cctctttaaa
      241 gggaatggaa tttcttatgg caggtgacaa aacgatgatc tttataatcg gtattccgat
      301 tgggatttta ttcgttgtaa tcagcttctt cctgtcgctt gcatgttata aaaaacgtta
      361 ttcataaagg aggcttcttt cccggcatac gtcacttgtc aatatcttcc agttttgtct
      421 ttccgatcat gagatttaaa atctccacat cggtctgttt catacctgcg cggcctacat
      481 atccgactgc cttgattgta tcttcagggc agtccattac aagaccttct cctcttgcaa
      541 acgtaagtcc ccgcatcgcc atcttatgac ttagtatcgc ggcctgaagc gccgtggata
      601 tttttgccgc acagcttggt tttgcaccat cacagacaat acctcccgca tccgctatcg
      661 tattgacaac tgtgttttca atctgttcca atgttccgcc gcataaatat gtaatccctg
      721 cccctgctcc tgccgcagca catactgctc cgcagtacgc tgagagagaa ccgatatatt
      781 ttttctgatt cagagccaca aggttagata cgcataacgc cctgtaaatc tcttctttgc
      841 ttttctttag ttcttttccg aactcgatca caggaagaga acaagtcata ccctgattcc
      901 ccgaaccgga attaatgatc accggaaggg cacagccgct cattcttgca tccgaaccgg
      961 cagatgcttt cgccacagcg cgccacttta catcttctcc gaactcttct agcacaactc
     1021 gtccgacctg tgctccccaa ggatttctca aaccttcttc tgcgattctc gtattgtatt
     1081 ttatctgacg tccaagaact ttttccacat cagtaatctg tacctcattt gcaaatgtaa
     1141 taatatcttc caaattcaaa agactcttat ccggtgtttt ttctctctcc tgacaatatt
     1201 cttttttctc tttgtgaaaa agcacctgtc cgtctttttc aatatgggtg atattcgtat
     1261 gctcgtcttc tatcacaaca agagcttcac tcttctctgt ctttttgcat actgcatatg
     1321 cttcaatata taaattagct acatcgtctt ttaaaacaca atcacaaaat cctgccttta
     1381 aaagtttctc cgcctcgtcg atttcccgat ctgtaacatg ctccagcact tcaagcgcct
     1441 gtgaaggatc tccgcctgta attccgagta ccgccgctgc ttctacgcct ttcattcctc
     1501 cggaattcgg tacttttact ccttttacat ttttgataat attaccgctg caccgcatcg
     1561 taatatgatc cggcatcttt cctaatactt cttttgcttt tgccgcagca taagcaagcg
     1621 cgatcggttc tgtgcatcca agcgccggaa caagttcgct ttccaaaatt gttacatatt
     1681 cattatatat tttacggttc attgcaaaaa acctcctgat ttctcgattt tctgatgtat
     1741 cttataacag cagtctgtct gatcctgccg ctttctttac agctttacac catctttatc
     1801 gattatacta tcaggaggtt tttatatcaa tctctttctt ttacgtgtct ttccggcacg
     1861 gatgtacacc gtttattccg tcctacatct ttttattttc tgtctttccc gcactcgtcg
     1921 cgcagcctga acacgaacca cagtttccgc cgcatccggc acacccgggc gcttttccct
     1981 cttttgcgtt ttttcttgct ttataaataa gaaaaataca ataactcgcg atcaatgata
     2041 tgatcaaaat atctgctgcc ataacacgcc ttctttctaa actaattttc tctacgccgc
     2101 ctgtaccgac cgtttggaat atattttttt atttttatac ggatccggcc taaacagtaa
     2161 gatcaacagg atcaccgcaa tcgctatacc tacagccgtt cctacggtga acgctcctcc
     2221 aagcacaaac gtaccgatct gatatacgct caaagatacg acatacgcaa atacattctg
     2281 gaacacaacc gcaaaccaaa accactttct ggactgcatc tgctgcgcca ttgttgcgat
     2341 tgccgccaga cacggggagt ccaaaagatt aaacagcaaa aacgaaaatg ctgccatagc
     2401 cgacggaaac cacgctgcta ctttgcccgc tacatttacg gtatcttcta catctcccga
     2461 cacatttgca agaactccca ttgtagttac aatggcttct tttgccgtaa atcccgaaag
     2521 cgctgccgca acaggctgcc attctccgaa accgagcggt ttaaacagca ctgcaagcgc
     2581 tccgccgatc atcgccaaca aactattggc actgtcttga acaatcccga attttccgtc
     2641 tgaaaatcca aatcctccaa ggaaccacat aacaatacat gcaaggaaca aaatcgttcc
     2701 cgccttgatc atgaatcctt tcagtctctc ccatacatga agaagaactg tctttgcctg
     2761 cggcatatga tactgcggaa gttccattac aaacggagcc ggttttcctg aaaacatctt
     2821 cgtctttttc aatattatag cagaaataag cactgccgca atccctataa aatacataag
     2881 cggtgcgatg aaggaacttt ctgcatatcc tgccgcctct cctgcgatca ctccgcccat
     2941 aagcgcgata accggaagct ttgccccaca tggaataaac gtagctgtca tgatcgtaag
     3001 ccttctgtca ttatcctgct cgatcgtcct tgaagccata atccccggaa ttccacaccc
     3061 tgaggaaata agaagaggga tgaagctttt ccccgacaat ccgaaatgtc ggaacacgcg
     3121 gtccattaca aaggcgatgc gaaccatata tccgcagtct tcaaggaatg ataaaaggag
     3181 aaacaatacc gccatctgtg gaacaaagcc aagcacagct cccaatccgc ctatgattcc
     3241 gtcaactaca agccctgtga taagactgcc tgcccctata tttcccaaga aaccgctgac
     3301 tgcatcctgt atactgacta caaacacatc gtttgtccag tctgtcatcc ctgttccgac
     3361 cgttgtgacg gacacataat acacaaccca cataacgaaa acaaaaatcg gaaggcctaa
     3421 aaaacgattt gttacgatac ggtctatctt atctgagaca gtcagttttt ctctcccttt
     3481 ttgaacggta tcggaaacaa ctttttgtat gtactgatac ctctcgtctg taacaatact
     3541 ttccatatcg tcatcatgct tttcttccat cgctttacgc atcttctcga tcgctttcaa
     3601 agactcgcct gacattttta aattttctaa tactttcctg tcattttcaa gaagttttac
     3661 tgcataccaa cgcttcttac gctcgtcaat ccatgttgga agcagctctt ctaccgctcg
     3721 tatcgccgct tctaactctt ttgagaatat cgctttcggt atgctttttt ctccgctttt
     3781 gacagtgcag accgtttctt cgatcaattc ttttagtcct gtctttttca aagccgacgt
     3841 ttcgatcacc ggacagttga gaagttcaga caatcgcttc ttgtcgattt tcattcctcg
     3901 cttccggatc aaatcagcca tatttagcgc aatcacaacc ggaactcccg tctcggtcaa
     3961 ctgggtcgtc aaatacagat ttcgctcaat attggtcgcg tctacaagat tgatgatcac
     4021 gtccggatcc tcgttcaaaa tataatccct gctcacaact tcttcaagcg tatacgggga
     4081 aagagaatag actcccggaa gatccgtcac tttgatcttc tcctcttttg attcttttgt
     4141 tcttactttc ccttcttttt tctctaccgt aactcccggc cagtttccga catattgatt
     4201 tgctcccgta agcgcattaa acattgtcgt ctttccacag ttcggatttc cggcaagtgc
     4261 agccctgatc tccattttgt tctacctcct actatacagt ttctacaagg acacattttg
     4321 catcctcttt tcttactgac aactcatatc ctcgaaccgt aacttctaca ggatcgccaa
     4381 gcggcgcaac cttccttata taaatcctgc ttcccttcgt gattcccatg tccatgatcc
     4441 gacgcttata agccccgtcc ccttcaattt taatcacagt tactgtactt cccacttttg
     4501 cgctgcctaa tgtcctctcc atgccattct cctccgcttc ctatttcact caaaattttt
     4561 atagcctttt cagctctaga tcataatatg cctcgccatt tcggcattta ccgcgatcct
     4621 ggaattttta atactcacga tcacgtttcc gcccatctcg gataatacag taacttccgt
     4681 tcctgccaca aaaccaagat ttccgagaaa acgccttgtc tcctcgtttc catttacgcg
     4741 gtttatcaca cttaccttgc ctatttcagc catcgtaagc ggcataaaaa caccttcctt
     4801 ctattttaaa acttttttga aattgataat ctttttcatt tatctttagt tagtttaacc
     4861 taacctttgt aaattgtcaa gtattttcac aaaataaagc acaaagcgtt ttttctacag
     4921 tgacgctctg tgccttttct atagcggata ttctttctct tgcgagcttt cgcacacata
     4981 tgttatactt aattttaatg aatattgatc tttcattcat aaataaaatt tatttgaggt
     5041 aacattatga ttataaatga atctgctgag aactatttag aaacaattct tgtattaagc
     5101 ggacaacttc ctgttgtccg ttctgtagac attgccgcag aacttgactt taaaaagcca
     5161 agtgtcagcg tcgcaatgaa gaagctgcgt gaaagcggac atattacagt ttcaccggaa
     5221 ggctatatta cactcacccc ctcgggaaaa gaaatagcgg agcgtatata cgaacgacat
     5281 accctcctta cttcctggct ggaatatctc ggcgtcgatt caaaaaccgc cgctgatgac
     5341 gcctgccgta ttgaacatgt catcagcgcg gaaagctttg acgcaataaa aaaacatatt
     5401 aagcgatagc ttttattttt aatttcggta atatactttt atttctcaat attgacctga
     5461 tcaagaactg cgcctaattc ctctgcggat ttacgcagtt ttttcatttc ttcctcgctg
     5521 aaatcaatct ccagcacctg ttccgccccg cctcttccga caatcgtcgg aatgcttaag
     5581 cacagtcctt ctaatccgta ttttccatca agcgataccg aaatcggagc gaccgtatga
     5641 ctgtttctta cgattgcttc tacgattttt tccgctgcca ttccgattcc gtaataagtc
     5701 gctccctttc gctcgataat ctcataggcg ctgtcacgga ctcctttgta aacctcttcc
     5761 atatcctgtg cgaagtgttc atatccccgc atctttgcaa aatcctgtaa tttaattccg
     5821 taaatatttg cacagctcca tgctgccagt tcgctgtcac cgtgttcacc tacgataaac
     5881 gcatgtacat ttctgctatc tacatccaac ttttcactga ttatatattt caatcttgcc
     5941 gtatcaagca ctgtaccgga accgatcact ctttcctttg gaaaaccgga ttctctaagc
     6001 gctacttccg ttaagatatc taccggattc gacacgatga gcaaaattcc ttcacagttt
     6061 acgcgtttga tctcttttat gattgaccgc ataatcgctg catttttctg cacaagatcc
     6121 agtctcgtct ctcccggttt ctggtttgcc cctgccgtaa taataacgac cgacgcatct
     6181 gcaatatcct catattcacc cgcataaata tccattgcat gtgtaaacgg cagtccgtgg
     6241 ctgatatcca tcgcctctcc ttctgccttc gccttgtttg catcaaggag taccatttcc
     6301 gaaaataatc ctttctgcat caatgtatag gcaatcgtag agccgacaaa cccacatccg
     6361 attactgctg ctttttgtat atttaccata tctcaatacc tcctgttttc catgaacttt
     6421 aatgaactca taccgccact ttatattcag gatctcataa taagcgtttt tttctaatat
     6481 tacttgatca tatttcatcc ttttttaatt cctagtattt tagtggggtt taactttatc
     6541 ttcattatat agtagttccc tgaaaaatca atcccttatc aggtattttt ttccatacaa
     6601 tcaaatattt ttcagtaatt cttttttatc agtcttctaa agaatgcttt tttgtaatcg
     6661 ttactttttc atcataacag ataaaaacgt cttctatctc gctttttttc agcttcggtg
     6721 tgatcaaact gataaaaggc acaatcaaaa gtcctgcgat cattgccatt gcgcctgcat
     6781 tgatcggaga ttttataaat ccaaaaaaca tattcgcaac agtcagtccg actcctgtta
     6841 taaaccctgc ccatacaccc gcacgggtaa cgcctttcca atacaaacca tacaaaaacg
     6901 gagctaaaaa cgccccggct aacgcgcccc atgaaatgcc cataagctgt gcgataaagg
     6961 tcggcggatc aagcgcgatc acaactgaga caacaataaa aaacacgatt aaaacctgca
     7021 ttgtcacaat ctgctttttc tcgctcattt ttttcataat attatctttc aaaagatcga
     7081 gcgtcagtgt agaactggat gtcagcacaa gggaggaaag agttgacatt gacgcagaaa
     7141 gcacaagcac aacaacaacc cctataagca gatccggaag catggaaagc atatgcggta
     7201 taatactgtc ataaacgacc gctccgcccg ctttatcgta aatctcggga ctgtcgaaca
     7261 gtcttccgaa tcctccgaga aaataacatc cacctgagat aataattgca aatatagttg
     7321 aaataatagt tcccgtactg atcgattttt catctttgat cgcataaaat ttcccgatca
     7381 tctgcggaag tccccatgtt ccaagcgaag taagaagaac aacgcccaga agattgacag
     7441 gatccggacc aaaaaaagat gtaaacgctc ccggctgtcc ttgtgtcagg ggaacatcac
     7501 ttggtatctt cgccatttct gctattgcat tcatgaatcc gccctgtcca ttcagaaccg
     7561 ccgcgatcac agccacaatt ccgatcagca taatgattcc ttgtatgaaa tcattgattg
     7621 ccgtagccat atatccgccc aggataacat agagcgccgt aaacgctgcc attgtcacga
     7681 cacaccacgt atacggaata ttaaaagcca tcgcaaacaa acgtgacaaa ccgttataca
     7741 ccgaagccgt gtacgggatc agaaaaacga acacgatcac agatgccgta atcttaagtg
     7801 atttgctctt gtaccgttct ccgaaaaaat ccggcatcgt ccttgatcca agatgctgtg
     7861 tcatcacttt tgtacgcctt ccgagaacta cccatgccag aaggcttccg attaatgcat
     7921 ttccgatgcc gatccatgta ctggcaattc cgtatttcca tccaaactgt ccggcatatc
     7981 cgacaaatac aactgccgaa aaatacgatg ttccatatgc aaatgccgtc agccacggtc
     8041 ctaccgatct tcctcccaga acaaaaccgt ctacacttgt aacatgtctt ctcgaataaa
     8101 ttcccactgc cgccatcact gcgaaaaaaa taataagcag tataatttta attcccatct
     8161 tttttccctc tctctgccgt tttatccttt agaccacttt aaagcgtaac actttaaagc
     8221 attaaagtat ctttttatag aataacacgg gtctaagacc cgtgtcaact gttacttttt
     8281 tctattatgc ccgtttatct tcccgtttta tcttgtcagt acatctcctg tcacttcatc
     8341 tcctcgaccc ggttttgcac cttcttatca aagttttcta tttgtctgct gtatgactcg
     8401 ttcatatatt ttgaatgaag cataaagtcc gcagtcgcaa gattatttgc aatcggaata
     8461 tcgtatacga cagcaatacg cagcagcgct tttacatccg gatcgtgggg ttgtgcttcc
     8521 aatggatccc acaaaaagat cataaaatca atctgccctt ctacgatctt cgctccaatc
     8581 tgctgatctc cgccaagcgg accgctacag tatcctttta ccggaagccc tgttcgctca
     8641 gcgataagac gcgccgttgt tcctgttccg cataaaaaat gccctttcag tatctctttg
     8701 tttttttcac accagtctac aagttctctt ttcttaccgt catgcgccac aagggcgata
     8761 tgttttactt tttcaatttc catcgttaca tatttatcat ctaacatccg atctcctcct
     8821 tttatcttaa aaatagtttt tcataaaatt tttcttaccg tctgttttta tacaaactgt
     8881 tccatcgtat caaatctaag taatgcgatc gcgcccatcg caaccgtgat catcaatcca
     8941 tagatcaata cgatcggaaa gatcaacgtt tgactgatca acactgccgc caacactccg
     9001 acaccggctc cgactcctaa aagcgccatc tggataaaca ttctaagcag actttgccct
     9061 gtttttccga atacttcccc gagaaggctc tgaacgatca ccattgtata caggcggtct
     9121 gcctgcaaaa cggtgtagat caaaatacag taaatgatct ctattaccgg aacatgccaa
     9181 aaaatcccaa taggaatgca gatgatacat ccgtccgcaa atgcctttac atgttccata
     9241 agagtcgcat accacatttt tttcagcgga ctgtcaggaa taagaaaaat atacggattt
     9301 ttcaactcgc tttcccattt tcctgtgtaa ccgctcataa taagcgccac atacgcgaca
     9361 actcccaaca aaataaagcc cgccatactg ctgtctgaca ccgatttttg gagagaatac
     9421 gcgaaaataa acgcaacgac tgccgaaaga actgtcattt tcgtgaaaat aaaatatttt
     9481 tctttcttat attccagaaa ttgcctgtaa aaaatcgctc ttgctccttt tgctgtaatc
     9541 ttactctcta cacggcgaaa ccttcgcttt ttcgtcccgg tatttgtcac aaattctccg
     9601 cttttctgcc tctttttcag ttctgcataa tcgtctgcga atttcgccgc ctcttcataa
     9661 tatccgccgt cacatttcat tctgaacgcc gccgcaacaa tgaacaccgt aaacaccgaa
     9721 tagatcacag tacaaattac attcagagtt gtcggaccga gtaaaaccaa cctgtatacc
     9781 gcaatctgcc atccgactac cggaatcatc tgaaggaccg gccagttgat aaacgaaaga
     9841 gcgctttcta ctgacagacc tttttcttta aaataaagta cgatcatcaa cgtaaatgca
     9901 atcaaaaaca ctttcattcc gaagcagatc ccttttatca atttttgcgg aagcttatca
     9961 ttcgtatata aaatgatcat aacagattgt tctaccgcac actctacgcc gcatcctatc
    10021 agaaagaaga aaagcatctt ccaccattcc acatgaaaaa ccgtcaagcc tccgatcgcc
    10081 aacacgatcc atatgaaaaa tgacatcaga taattcatcc acgctccctg tatcaaaaca
    10141 agtttggggc tgatcggagc gggaaatacg aactgagaat gagccggccg aaacaagatt
    10201 ccttttcgag aagcatagga cataaagttt cctactgtaa tgtagatcgt ccacaccgtc
    10261 acaagaatga caagtccttg aacagaatcg atccgaactg taaccgcaag tgttccaagc
    10321 atcacgataa gaaagattcc ataggcggcc ccaaagatca acgcaagaag cgtcgtcggt
    10381 tttctgactg cctttttcag gttatttgcc gctgttctct ttatcaaata tattaacgct
    10441 ttcatgcttt ctcaccgcct gtcatctcaa agaacagttc atccagctct tttccctgcg
    10501 cgtctgcttt tgtgtaagaa ccggcaatat gcccttgttc catgacgatc atcacatccc
    10561 acaagttctc taccatctca agcatatgcg tactgataag aactgtcgct ccgttatcac
    10621 gcagttttaa cacgacatct ttcagcatct tgatcgccgc cggatcaagt ccgaccatcg
    10681 gttcgtcaag aaggatgact tccggcttta ccgcaagggc acagcatatg ctcactttct
    10741 gcatcattcc ttttgataat tcattgccaa gcttatcctg cttgtcatcc atctcaaacg
    10801 ctttaagtat cttttcgatc tcctcgtctg taatttcact gtcatatgcc attctcacat
    10861 attcgatatg ctcccttacc gtcagcgcat cgaacatatt cggaatctcc ggaacataag
    10921 caaacttcct tttcgcagga atctgccggg caggaatccc tttaatacga atttcaccct
    10981 gataccgcaa taaaccggca atgcttttga tgatcgttga ttttcccgat ccgttcggcc
    11041 ctaaaagaac ccctacctga ccgttaggga cggtaaacgt cacttgatct accgccagat
    11101 tctttccata atattttgtc aattctttga tctccagcat agtcctctct accctttctg
    11161 tccgcgtcct gtatagtttg cttttactcg taatacttga taagtgcctg tgttccgaga
    11221 tctccgttgc cgtctgcttc aagctcttca taaatggcaa gaacctggct taaaacttcg
    11281 agatttaaac cgcttctgtt tgcttcgatg agcgccagct tcatatcttt aataaaatgc
    11341 ttcataaaaa atcccggcgc ataatctttt tctatgatct ttggtccgaa cagatcaaac
    11401 tgtctgcttc cggctgcccc tgtcgctacg gatttcgaga acacattcag gtcaagtccc
    11461 ttttcttttg catatgtcag cgcttcgcat acaccggaaa gagctcccgc gatcatgatc
    11521 tggtttgcaa gtttgcaatg ctgtccgcat cctgccgaac cttcgtaatt aatattcgtt
    11581 cccattgctt caaacagttc atgacagttc gtataatcgc tcttatcgcc tccgacaaga
    11641 atagaaagag ttccttctct tgcccccgta tctccgcctg tgaccggcgc atcaagcaca
    11701 tgaaatcccc gttttgttcc ttcttcatac agcttttcgg caatctgcgg actcgtggtc
    11761 gtcatatcga tcaaatatgc accttttttc gcactgtcca aaatatttcc gctgtcgaaa
    11821 tatacttctt ctacatcctg cggaaatcct acgatcgtaa tcactgcctc gcagtctttt
    11881 acgcactcgg aaatcgtttc atgaaagatc gctccttccc cgatcacgtc ctcgactttt
    11941 gcttttgtcc gggcataaat atgaagttca taccctgctt tcataagatt acgcaccatg
    12001 gattttccca tgatccccac accgataaat ccgatcttct tcatctcttt tttcctttcc
    12061 cccttatatg ccgctatttt tacgacactt ctgtttttat ccacatcggt ttgtctgtct
    12121 tgcctgtatt aatatttttc tacgacttcg cttttgtttt tataaatact gtttgccgga
    12181 acaaatcctc ttacagaaga cagtgggtaa atattgctgt gacttcctac aaccgttccg
    12241 gggttcagga cgcttccgca tcctacttct acttcatctc cgagcattgc tccgaacttc
    12301 ttcatgccgg tttctatatt tccttccgat gtttttacaa caacaagctt tttatccgac
    12361 ttcacattcg atgtgataga acctgctccc atatgagatt tatatccgag aacagaatcg
    12421 ccgacataat tataatgcgg tacctgtact ttattgaaaa gcacgacatt tttaagctct
    12481 gtcgaatttc ctacgactgc tccttctccc acgatcgcgt ttcctcttat aaacgcacaa
    12541 tgacgcactt ccgcttcttt cccgatgatc gccggaccat taatacatgc ggtaggcgca
    12601 actttagctg actttgcgat ccagacatct tctcctcttt tttcatattc atcactgctt
    12661 aacgtattcc cgagttcttt gataaatgaa ccgatattcg gaaggacttc ccacggatat
    12721 gtcactttgt caaaaagatc ttttgcgatt gtctcctcta atgtataaag ttcctttact
    12781 gtcaatgcat tcatatattt atctcctttt ccgccgttta ccggctcttt ttataataac
    12841 ctgccgcttt atcataacag gctcttaact gatactgatt attgagtcct tttactccca
    12901 ccgtcctgtt cgttataaaa cgatccagct tcatcatata ttcatcggga gtaatgcttc
    12961 cctccgccac atacgtaaga cctttttccc agcttgccgt aagttccgga tttaaaagtg
    13021 atttgatcga atgatccact acgtcaaata ccatctctcc ttgcaaagtc ggtgttatga
    13081 tctgtgtttt tttattcagg gcaaggtatt taatatgaat cagtttcttt aatatttccg
    13141 ccctggtcgc gctcgttccg ataccgcttc cttttatctg agcgcgaaga tcttcatcct
    13201 cgatcaactg ccccgcattt tccatagcaa ggatgatcga tcctgaatta taccgtttcg
    13261 gaggcgacgt ctccccttct tttatatcta atgctccaac cggaagaacg cttccttttt
    13321 tcagcgtttt aatcacttca aacagcgccg tatcaaggtc ctgatctgcc gtctgttctg
    13381 catcttcctc tttttcattt gcatcggttc tgcttttgcc tttgtttaca cttcgttttc
    13441 cctgcggaac gcctgccact ttcaaatatc cttcctcagc tagcacttta aaattggaaa
    13501 agaaactctc ttcctttatc ttcgatatga tcgccacttt ttgatacact gccggcggat
    13561 aaaaaatact taaaaacctt ctcacaatca gatcatatac tttatgagac gttgccgaaa
    13621 gcgaattcag cgctcctaaa ccttgtcctg tcggaatgat agcgtaatga tctgtaatct
    13681 gcttatcgtt tacatatctt gtcttcgcca gatttttatg acttccaaaa gaaaggatat
    13741 cctgcaaata cggctgtgcc ggaggatatt ttgacagccc gttcaagttt ctgctgatct
    13801 cttttgcaac tgccgtcgac aaaaccctgg cgtctgtcct cgggtatgta acaagttttt
    13861 tttcatataa ttcctgcacg acccgaagcg cttcatccgg actgatctta aaccgcttcg
    13921 aacagtcgtt ttgaagttcg gccagattat aaagaagggg aggatttttc ttctcctttt
    13981 tcttctccac agactcaacg atacactgta tcggcttttc ttccaacaaa taggcgatca
    14041 gctcttttgc tttttcttct gtcttaaatc cattttcttt atacagatca aacgattcaa
    14101 aataccgcga gcctttcact gcccgccatt ctccttcaaa actgtgacct gacgcatcga
    14161 tcgtgctgat cacacggtaa aaaggagttt ttacaaactc ccgtatctcc ctttcacgcc
    14221 tgacaaccat tccgagaacg catgtcatta cacgtcctac cgaaatcacc gagtatttcg
    14281 tattcagata attagaaata ctgtttccgt atttcagcgt aagcaaacgt gaaaagttga
    14341 ttcccatcag atagtcttct ttcgcgcgaa gataggcgga agcgcttaga ttgtcatact
    14401 ctgacagatc ttttgcttcc cgtatgcctc ttaagatttc ctcttctgtc tgtgagtcga
    14461 tccacacgcg aagtcttttt ttccccgaca cctgcgcctt ttgttctaca agccggtaaa
    14521 tgtactctcc ctcccgtccg gagtcggtgc acacataaat tgtgtcaacg tcttctctgt
    14581 ttaaaagaga actgacaatc tgaaactgct tcgccactga cgggatcact tcatatttaa
    14641 actccgaagg aataaacgga agtgtctgaa gactccattt tttcaatgcc gggtcatact
    14701 cttccggata gctcatcgta accagatgtc cgacgcacca tgtgatcact gcttcgtcgg
    14761 attctaaata tccgtccgct cttcttgtct ttaattttaa tgcctttgcg aactcctgcg
    14821 ctacgctggg tttctcggcg atatagagtt gttttttcat ataatgtatt tagtttcctt
    14881 tcctgctccg aaagtatttc tcaccttttt tcaacacgat ctattccttt ttccaaatgc
    14941 gctgaatact ttctgaaaga ttgatttttt ttcctttttt gctttcggaa gcgtcttcgg
    15001 agggcgtgca ttcaacgcag ctttcataaa tgcagcctgc gcgttgaccg cttctccttt
    15061 tctttctttt ttcttatatg taccgtcact tgtcataata cgcgctttta cattgtctgc
    15121 aagcatcact ttcagatcat ggatcaggcg tctcttaatt gcatcgtcat aaatcggaca
    15181 tgcaacctcc acacgtttct ctgtatttct cgtcatcata tctgccgatc cgatataaac
    15241 tttcgccgtt tctcctgttc cgaacacaaa cactctcgga tgttccagat accttcctac
    15301 aatgctcgta acccgcagat tttccgtatt ttctttcact cccggcaata tacagcagat
    15361 tccgcgtacg atcagatcca cttttacccc tgctgcagac gcttcctgta cttttcggat
    15421 aaaatccata tccgttacag aattcatttt cataataata cgtccatccg cgcctttttg
    15481 aatttcctca tccatatgtc ttaaaacttt ccgtttcaga ctaaacggtg aaacgatcag
    15541 atgatgatac tctccgtcca gattgccgat tgacatattt ttaaagaata cggccgcatc
    15601 ctcgccgatt ccctgatgtg atgtcataag cgaataatct gtatacattt ttgccgtctt
    15661 ttcattatag tttcccgttc ctacctgggt aatataacgg atctcatttc gattcctgta
    15721 tgtgataaga cacagttttg aatgaacctt gtaaccgtcg aatccataca gcacacgaca
    15781 tcccgcttcc tccagacgct ctgaccagtc aatattattc tgctcgtcaa aacgcgccct
    15841 aagttcgatc aatacagtta cttcttttcc gttctcggct gccgcacaaa ggtattctac
    15901 aaggcgggcc tttttcgcca gacggtagat cgtgatcttg atcgtcataa catcgggatg
    15961 aaacgcagct tctttaataa gctgcaaaaa cggatccata ctctcatatg gataagacaa
    16021 caaaacatct ttgcgtttca cctgctccat cacgctgatc ccttcggcaa gcgcagcggc
    16081 aggctgaggt gtaaacggct cgtcgatcag cgcttttctc atagattcgg gaatcttatc
    16141 tgcgattgcg aaaataaacc caagcttcat cggcattttc gtcctgaaaa tacattccgg
    16201 cgtaatctta aaccggtcgc agaaatattt ttccatctct tttccgagcg gagcggctgt
    16261 ctcaagtctt acaactgcca ttttacgtct tttatgaagt gtttctttca taatataacg
    16321 gaaatcttca ttatctgcca acgcttcatc atccggtgcg atatccgcat tccgcgttac
    16381 gcaaatatag tttttgtcag atacctgata ctgttcaaaa acaagttcca gatattccat
    16441 gatcactttt tccattcgga tgtagcgcac atcatgcccc ggaagataga gaacgtccga
    16501 aatgtactgt ggcacaggca caatgccgag catgacatta ttttccattt tcagcgtcgc
    16561 cacgacgtaa atatctttat taagcagatg agggaacgga tggttcgcat ctacgatctg
    16621 aggcgataaa accggaagca cctgttcttt gaaatatttt ttcacatact tcttttcctg
    16681 ctgctccaat tctttaaaat ctaacccaca tacgccatac ggctgaagct gcttttttat
    16741 ttccgcatat gtcttatctc tctccttata aagaggagct accgctttat aaatctcctc
    16801 caactgctgc ttcggagtca ttcccgaacg gatatcctgt tttttactgt cagctactgc
    16861 catatcgtaa aggctgccga cacgaatcat aaagaattca tcaagattac ttgtaaaaat
    16921 tgcgacaaat ttcatcctct ccataagagg aacactttcg tcctgcgcct cttccaacac
    16981 acgctgatta aaacgaagcc atgataattc tcgattttgc gtatactcta aagtctctgt
    17041 ttttcccatc ataccctctc ctttttgatt ttccgacatt tctcatcttg taaactatcg
    17101 tttcatttat cgtgaaaaag atttctgctt ttatttttcc aaatattcca taagatcatc
    17161 aaactttcgt tctgtgtact tatatccgtg ttcataaaat gcatgcagcg tttctttatt
    17221 tctctcaagt cttgaaacag gctttacctg cggtctgaga ataaatatct cacctcgttt
    17281 ttcaagctga tctaaataat tcatcgtttt attatactca aaacttcttc tgaagatcgt
    17341 cctgatcaaa ttcggatatg atttatacgc tcttttatat actctctgca tcgtcggaga
    17401 aagaaccgac ttcctgtaac cttgattttt cgtaagaatc acgacgatct tttcattttc
    17461 catctgctgt gcccgtctga tcggaacaga gtccgccagt cctccgtcaa ggtacggaac
    17521 attgtcgata ttaacgatcg gagtaagcaa aggcatactt gacgacgctc tgcaaagttt
    17581 catcagacgg tcgttatcac tatcctctgt catatactcc gcttttcccg tcagacagtt
    17641 tgtcgtaaca atctgacagt tgatctccga gttaaaatat gtctcgaaat caaacggaag
    17701 cagttctttc ggatatttat caaagatcag atccatattc atcagacttt tctctttgac
    17761 gaagtctctg atcccatagt aatattttcc ttctttgtca gtcggtatca tacagtctct
    17821 cgttcttccc ggctgccgcg aaacatagtc caccgcattg cacgagcccg ccgaaacgcc
    17881 gatcacatcc gaaaaataca gatctctctc catcagataa tcaagcgctc ccgaagtaaa
    17941 tattcctctt gtcgctccgc cttcaagaac aagtgttgcc ttctttattg tcatatggat
    18001 aacctctttt ctgctacaat ctacttccct cttttaactt ctacattata taactttttt
    18061 ccttattgtg aaaccagaag ttgattttta aacaaatctt ttcaaattct tttcaaaatc
    18121 tttacttttt gttgtaggtc atatccagaa gcttctcctg ccttatccga caataattca
    18181 ggttttttca taaataaatt accggccttc ctttgcttta ttttttactc aacagcgctt
    18241 tcacttcctc tacagtcaat actttcccat aggatataac ttcgccgtta aacactaatg
    18301 caggtgtcgt cattactccg taaccggcga tttccgcaaa atctgtaaca tgttcaatct
    18361 cataatctaa tcgaagttca gatactgccg ctctggttgc tttttctaat tccatacatt
    18421 ttacgcagcc cgaacccaat attttgatcc cttgctcttg tttcttattt tctgtatttt
    18481 gcattgtttc tgacgtgcaa ttttcaccac aacagcatga ttttgtttct tttttctttc
    18541 caaataattt catttcttat tcgctccttt taaataaata tcgcactaaa aatattaaag
//
LOCUS       DBMDXF010000012        18486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_924_length_18486_cov_2.206418, whole genome shotgun sequence.
ACCESSION   DBMDXF010000012 DBMDXF010000000
VERSION     DBMDXF010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 18486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18486
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_924_length_18486_cov_2.206418"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="ACZPD4_01045"
     CDS             <1..540
                     /locus_tag="ACZPD4_01045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR1377698.1"
                     /translation="AGIIVLEELEHAKARGAKIYAELAGYGATADAWHITSPAEDGEG
                     AAMAMKLAMREAGVRAEQIDYINAHGTSTHHNDLFETRAIRSALGSAADQVVVNSTKS
                     MIGHLLGAAGGVEFIVCVKSIQDGYIHQTMGTKNVDPECDLNYAVGSPVKKDVNYVLT
                     NSLGFGGHNASLLVKKYED"
     gene            555..992
                     /gene="accB"
                     /locus_tag="ACZPD4_01050"
     CDS             555..992
                     /gene="accB"
                     /locus_tag="ACZPD4_01050"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR1377699.1"
                     /translation="MKTEQILQLVEAVSGFGLDEFEYEEDGVKLKLKKGGNVCTSVSA
                     ETAGRTKTEAEIARTEENAEVLLEGKIVKAPLVGIFYASPEEGGTPFVQAGDKVEKGT
                     VLGIVEAMKLMNEIESDFEGTIEEVLVENGAAVEYGQPLFVIK"
     gene            1045..1491
                     /gene="fabZ"
                     /locus_tag="ACZPD4_01055"
     CDS             1045..1491
                     /gene="fabZ"
                     /locus_tag="ACZPD4_01055"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR1377700.1"
                     /translation="MERETKKSLNIEQIQQIIPHRHPFCLIDRIEDYEPGEFAIGYKA
                     VTYTEEFFKGHFPNKPVMPGVLIVEALAQTGAVAILSTPENKGKIAFFGGIQNCRFKG
                     MVFPGDVLRLETKIIRQKGPVGIGKAVASVDGKVVVSAELTFMAGE"
     gene            1496..2845
                     /locus_tag="ACZPD4_01060"
     CDS             1496..2845
                     /locus_tag="ACZPD4_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR1377701.1"
                     /translation="MIEKILIANRGEIAVRIIRACREMGIETVAVYSEADKEALHTQL
                     ADEAVCIGPAQASESYLNMQNLISAALVSGADAIHPGFGFLSENAKFAELCEQCEITF
                     IGPDSKVIGKMGNKTEARATMIAAGIPVIRGTNGAVLDLEEGKKEAARIGYPVIIKAA
                     LGGGGKGMRTAQSEEEFESCFVTARKEAEKAFGDGTMYIEHFVEHPRHIEFQILADSY
                     GNIVHLGERDCSIQRNHQKMIEECPCAALSDELRKKMGETAVKAARAAGYTNAGTVEF
                     LLEKDGTFYFMEMNTRIQVEHPVTECVTGIDLIKEQIRIANGGELTFRQEDIRMTGHA
                     IECRINAEIPERNFMPSAGRITDLHLPGGNGIRVDTAVYSGCEVLPYYDSMLAKLIVH
                     GKDRREAVDKMRSALGEIIIEGIGTNVDYQYEISENEDFISGKTDVDFIRRLSSETA"
     gene            2832..4559
                     /gene="accD"
                     /locus_tag="ACZPD4_01065"
     CDS             2832..4559
                     /gene="accD"
                     /locus_tag="ACZPD4_01065"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR1377702.1"
                     /translation="MKLHNMFKKTVGKEHGTSKNTKVRNVPEGLLKKCNKCGAAILAE
                     DAVNNNYICPKCHGYFRMHAGRRIEMIADPGTFEEWDAKMKFENPLEFSGYEEKVRRL
                     REQTGLDEAVVTGKVMIAGHPAVIGVCDGRFMMASMGEVVGEKITRAIERATKENLPV
                     ILFACSGGARMQEGIVSLMQMAKTAAALKRHSEKGLLYVSVLTDPTTGGVTASFAMLG
                     DIILAEPNALIGFAGPRVIEQTIGKKLPKGFQRSEFLLEHGFVDRIVERSEMKSVLAR
                     ILHMHDRTKEKTEESTGETEKIESTTQEDTMPKQVRSKGGISAWERVLLSRKTDRPVG
                     TDYVNMLFSDFMEFHGDRLFRDDPAVAGGIAMFGNIPVTVIAQVKGKTTKENVTRNFA
                     MMSPEGYRKTLRLIRQAEKFGRPVICIVDTPGAFCGQEAEEHGQGEAIASNLYELSSI
                     KVPIVSVLIGEGGSGGALALAVADEVWILENSVYSVLSPEGFASILWKDSKRASEAAE
                     VMKLTADDLKCLGIVEQVIREPKSYTSQTMAGVCEELKNMLSVFLKKYGSMDERQLLN
                     HRYDRFRKM"
     gene            4668..>4791
                     /locus_tag="ACZPD4_01070"
     CDS             4668..>4791
                     /locus_tag="ACZPD4_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR1377703.1"
                     /translation="MDAYRTINDTLVNLFNEIWELEKEAIITEEFKDITNNDMHI"
     assembly_gap    4792..4801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4802..5075
                     /locus_tag="ACZPD4_01075"
     CDS             <4802..5075
                     /locus_tag="ACZPD4_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1377704.1"
                     /translation="AKKLKITAGSLTTAVNALVRKAYVKRERSEEDRRVVYIALTEKG
                     EKAFYHHEQFHRQMTNAVIEKLDDEEIDALVKMLKDISAFFRSYQS"
     gene            5115..5753
                     /locus_tag="ACZPD4_01080"
     CDS             5115..5753
                     /locus_tag="ACZPD4_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR1377705.1"
                     /translation="MDEFIFILELIGTVAFASSGAMIAIEKKMDIFGVNVLGATTAVG
                     GGIMRDIILGLTPPGAFSHPVYVLVAALTSTILFVIAYAKPTAFESRVKTDYYDKLMF
                     WCDTAGLGIFTVVGIQAAVRAVGGENVFFFVFIGTLTGVGGGVLRDIMAGETPYILVK
                     HIYACAAIAGGIVCVVGRTAFGEAYGTILGLAATVLLRFLAAHFRWNLMRVS"
     gene            5967..6545
                     /locus_tag="ACZPD4_01085"
     CDS             5967..6545
                     /locus_tag="ACZPD4_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HIR1377706.1"
                     /translation="MAVKKTTSTSRAAAKIANAIKAEPAKAAKTAAKAVKEEEKTAVK
                     AEEKAEVKASEKAPVKAEPEKKEAAKAIETKKDTTKKLETKAAVLVDTPETEEKAAVK
                     KSVKAPAKAEKKAPVKKAASKKAELKTEMFLQFAGKEYTDKEIFDKIKYVWSKVLKNK
                     VGDMKDVKIYLKPEESAAYYVINGDTTGKVDL"
     gene            6627..7259
                     /locus_tag="ACZPD4_01090"
     CDS             6627..7259
                     /locus_tag="ACZPD4_01090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1377707.1"
                     /translation="MSENTKSKMTEKKEQRKRLEQQIRFIIEADKVKSVFRQTYLADG
                     SRKENDAEHSWHLALSAILLKEHMDAPVDLMKVIVMVLIHDLVEIDAGDTYAYDSEGT
                     KSKRERELKAAERIFGILPKDQGTYFRSLWDEFEEYSTEDAKFAHLLDNFQPLLLNDA
                     SDGKSWAEHGVHKAQVCKRNERIADTSDIVWEKMLEIMDKHVEKGNLKNV"
     gene            7252..7977
                     /locus_tag="ACZPD4_01095"
     CDS             7252..7977
                     /locus_tag="ACZPD4_01095"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1377708.1"
                     /translation="MYERETERLQKMIEESESIVFFGGAGVSTESGIPDFRSADGIYH
                     QKYRFSPEQVVSHTFFMRYPEAFYEFYKEKMMILDAKPNAAHLKLAELERAGKLTAVV
                     TQNIDGLHQLAGSKRVYELHGSIHRNYCMNCGKFYDAQYVKKSEGVPKCSCGGVVKPD
                     VVLYEEGLDEETIRGAVEAIASADMLLIGGTSLVVYPAAGFIDYFRGSRLAVINKSET
                     AKNIRSELTISAPIGEILSRIKV"
     gene            complement(7978..8856)
                     /locus_tag="ACZPD4_01100"
     CDS             complement(7978..8856)
                     /locus_tag="ACZPD4_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1377709.1"
                     /translation="MEKGYYYEIADNWKDYEVIDCSNGEKLERWGDYLLVRPDPQVIW
                     NTKKNHKGWKQMNGHYHRSKKGGGEWEFFSLPQQWQIHYGSLTFNLKPFSFKHTGLFP
                     EQATNWDWFSEKIRNAGRPVKVLNLFAYTGGATLAAAAAGAHVTHVDASKGMVGWAKE
                     NAVSSGLKDAPIRWLVDDCVKFVEREIRRGNTYDAIIMDPPSYGRGPKGEIWKIEDMI
                     HPLIKLCTKILSKDALFFLVNSYTTGLAPAVLTYMISTELKPWNGHVESQEIGLPVTD
                     SGLVLPCGASGRWESD"
     gene            9080..9829
                     /gene="yedF"
                     /locus_tag="ACZPD4_01105"
     CDS             9080..9829
                     /gene="yedF"
                     /locus_tag="ACZPD4_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HIR1377710.1"
                     /translation="MVKMTVDAMGDHCPIPVVKTKKALGKLQGAGQIEVLVDNETAMK
                     NVMKMAKSSGASAESEKISDREYKVMITVEEKNAAVSESDQKGQTAGKESRAQAEGGG
                     KEAGESDHNPAAENGSNSSAQGMNKVQLSSAGCATCIGTVVAVSSDKMGSGNDELGAV
                     LMKSFFFAETQLDTLPDKILFYNGGAKLTVEGSDCLEDIRILEKEGVEILTCGTCLDF
                     YGLKEKLAVGAVTNMYTIAEILQNAVSVVSP"
     gene            10076..13465
                     /locus_tag="ACZPD4_01110"
     CDS             10076..13465
                     /locus_tag="ACZPD4_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HIR1377711.1"
                     /translation="MKKTKKLVMSWLLAFCCVITLVAPSVVNAAADDFEIIDDTDARF
                     VYSDGNANNGGWESAGAGDASVTEHWSNTAGATLDITFNGTKLELYGKKAANHAMFSV
                     SIDGSEAVECDAYAAVTEPEAKLFESKTLAAGNHTAHITVLEKRNEASTGTGSVYGVQ
                     FVYAKAFEAEIAEPEFPGYTEVDDAVFTTNHEPFKIQYEPAGAWTAGSGNGNLFYNGT
                     EHYSAKGVDVSYEMIFTGTGVEIHASKNTVHMNCDVYIDDVKVGELAAQNAGAMHKQK
                     LFEKKDLANTKHTLRVVPKEGESIEGKVIQLDKIVVFHDEITVPDSIVLNRTELTMTP
                     GASSELTASVIPWNANAKLVWTSSDSSVVEVNDGKLTAKEIDVKKTVTVTAASAEDNS
                     VLAEAKITVDPALAFMNAYVGNEKLLETELDYDKLKAGNGSVYNGTAWLNDELNSKIV
                     VTTEKDVHNVQVTASDFKNEKGQVLSKDNIDIKWLKEIAAKEGRNAQGQTKNYPDVIY
                     KGGKKDIDAQDVQFAWVNIAIPKDTAAGNYTGTITVSADELDKPFELTYNIEVLNLVQ
                     PAPEATELQVWQHPFSVANYYLGLGENPSGGITNEVREDFYFTEKHFNLMRDSIKEYV
                     SIGGHDVVANVVEEAWNHQSYYNDLSMVKWTKKADGTWEFDYDWYDAWINFMIECKVL
                     DPANGIGQIKCYSIVPWNNQIAYYDEAQGKVVKESHNPGTAKWKEMWESFLKDFMEHS
                     KKMGWFDITYISMDERGLDQLEPAVEMIESVKDEDGNHFKISSALNYAAPEYYEFTDR
                     IDDISINLGNTGNVQQMNDLSDHRRDLGLTTTMYTCTGDYPSNFMISDPGDNYWDIWY
                     TMTLGTDGYMRWAWDNYVYDMHGDATYRYWEPGDGWFIYPMEREAVGEDFNASFYSTP
                     RYELFKQGIRDVAKAKYLLNSESATAEEKTELTDVVEHLAKPQKGTYQGSAVAASEKD
                     RMLVHSETERALDATNALARNVAERENPNPKPESADKTALNAAIKDAEALKKEDYTAE
                     SWKAFETALNAAKETAADKDAAQTEVDNALNVLNAAVAKLEKVKDPNQQQPVQPQPEQ
                     TKPDKTTPQTGDRTNAALLFGCAVLSGAGVFFAYRRRRTIK"
     gene            13681..14724
                     /gene="selD"
                     /locus_tag="ACZPD4_01115"
     CDS             13681..14724
                     /gene="selD"
                     /locus_tag="ACZPD4_01115"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR1377712.1"
                     /translation="MEKIHLTQFAKHGGCAAKIGPDTLGKVLGRLPKFHEDNLLVGFE
                     TSDDAAVYKLSDDTAVIQTLDFFTPVVDDPYTFGQIAAANALSDVYAMGGEPKIALNI
                     VCFPGELDPDYLGEILQGGAEKVLESGAVLVGGHSIQDDVPKYGLSVMGLVHPNRIYK
                     NFGCKKGDVLILTKQLGSGIVNTAVKAQMASEAAGKEAVAVMTQLNKKAREILDKFTI
                     HACTDVTGFGLLGHCGEMASASRAKIELWPKDIAYIEGAKEYAQMGLIPGGAYRNRNH
                     VAQLLEAGNTDEMYVDLLSDPQTSGGLLAAVPEEEKDAILEEFDKAQMETKVSVVGKV
                     IDSECEEAKIYIR"
     gene            14743..15879
                     /locus_tag="ACZPD4_01120"
     CDS             14743..15879
                     /locus_tag="ACZPD4_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1377713.1"
                     /translation="MIYLDNAATTLHKPKEVQEAVIAAFGQMGNSGRGASEPALKALG
                     TVYDAREALARLFHAENASEIVFTANSTEALNIAIKGILDPGDHVITTVLEHNSVLRP
                     LYECRRKGVEMTILTCDEYANISYEEMEKAVRKNTRAIICTHASNMTGNMISLESVGK
                     IAAKYGILFIVDASQTAGVWEIDVQKYGISVLCFTGHKGLMGPQGTGGMYVKSGVKIR
                     PLLSGGSGIDSYNMHHPSQMPEALEAGTLNGHGIAGLRAALSFLEREGMERLRKRELE
                     LMRRFYNGVAEIPNVKVYGDFRTDRRAAIVSLNIGDYDSAEISDELSGRYGIVTRSGA
                     HCAPLMHEALGTKIQGAVRFSFSHYNTEEEIDEAIRAVKELAEE"
     gene            15933..16169
                     /locus_tag="ACZPD4_01125"
     CDS             15933..16169
                     /locus_tag="ACZPD4_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HIR1377714.1"
                     /translation="MKKREKKIVITFHTTTDAMAMERYCKKRKEPGRLIPVPGTISAG
                     CGLAWCVLPQEREHLLKVMEEGDITPQAVHECML"
     gene            16341..17453
                     /locus_tag="ACZPD4_01130"
     CDS             16341..17453
                     /locus_tag="ACZPD4_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377715.1"
                     /translation="MIEVKNLVKKYGNHLAVDHLDFQIKPGMVYGFLGPNGAGKSTTM
                     NIMTGCMGATQGEVLINGYDILKEPEKAKRYIGYLPEHPPLYMDMTVREYLDFAAELK
                     GIRKSQRREAVDEAEKMVKVQDVEHRLIRNLSKGYRQRVGLAQAILGFPDMIILDEPS
                     VGLDPKQIIEMRDLIRKLAENHTVILSSHILTEIREVCDYILIISKGKLVAQDTLENL
                     EKMIGFHDVIELETSASEEEIHRILGRIPGVCAMQMRVKGEKCTYVQVKVTGDKSSQN
                     NRKVREEIFCEFAKEKKTLYSLSAPETSLEEAFMKLTQNDNIERNMQIEESLVKDRQV
                     KDGRVKESRPGEKQIGVETRKCEMNTEGGKEDEGGL"
     gene            17437..>18486
                     /locus_tag="ACZPD4_01135"
     CDS             17437..>18486
                     /locus_tag="ACZPD4_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1377716.1"
                     /translation="MKAVYKKELKAYFQSIIGCVFIAFLIAFTGIYFMAYNMTTGYPY
                     FSYTLSGSLIVFIVGIPLITMRSFAEERKNKTDQILLTAPVSLWKVVVGKYLAMVTVI
                     AIPNLIFCIFPLIIKLQGTAYLTVDYISILMFFLLGCVFAAIGMLLSALTESQIIAYI
                     GTFGIFLILYLWDGILSLFPNTAMSGLVFIILLILLAAFYIWRMTNSFSIAGGVGCVG
                     VAVAIAVYFLKETLYENLFTESLGGFALTNIFTDITQNSIVDVSGIVLYLSMITLFLF
                     LTVQSIRRRLWESAAKKHGAYSVTMTAVFLAITVVINLIACQIPEKFRKIDVSNTKIY
                     EISDTTEDFLKEMDKE"
BASE COUNT         5883 a         3148 c         5054 g         4391 t
ORIGIN      
        1 gcaggcatta ttgttttgga agaactggaa catgcgaagg caaggggcgc aaaaatatac
       61 gcagagcttg cagggtacgg agcaacggcg gacgcatggc acatcacatc accggcagaa
      121 gacggagagg gcgccgcaat ggcgatgaag cttgcaatgc gggaagcagg cgtacgggca
      181 gagcagatcg actatattaa tgcacatgga acaagcacgc atcacaacga tctgtttgag
      241 acaagagcaa tacgcagcgc acttggaagc gcggcagatc aggttgtcgt taactcaacg
      301 aaatcgatga tcggtcatct tctcggagcg gcaggcggag ttgagtttat cgtatgtgta
      361 aaaagcattc aggacggata cattcatcag acaatgggaa caaaaaatgt cgatcccgaa
      421 tgtgatctaa attatgcggt aggctcaccg gtcaagaagg atgtaaacta tgtattgaca
      481 aactcactgg gattcggagg tcataacgct tctcttctcg tgaaaaaata tgaagattaa
      541 ggcggggtgc aaaaatgaag acagaacaga tccttcagtt ggtagaagct gtgtcgggat
      601 tcggattgga cgaatttgaa tacgaagaag acggagtaaa gctgaagttg aaaaaaggcg
      661 gaaacgtctg cacatcagta tcggcagaaa cggcaggaag aacaaagaca gaagcggaga
      721 ttgcaagaac agaagaaaat gcggaagtct tattagaagg caaaatagtg aaagctccgc
      781 ttgtcggaat tttctatgcg tcgccggaag aaggaggaac tcctttcgtg caggcaggag
      841 acaaagtaga aaagggaacg gttctcggga tcgtggaagc gatgaagctt atgaatgaga
      901 tcgaaagtga ttttgaggga acgatagaag aggttcttgt cgaaaacgga gcggcggtag
      961 aatacggaca gcctcttttt gtcatcaaat aaagaacgat acaaaaacgg catagtaaaa
     1021 atatcaggga ggcagagtac ggagatggaa agagagacaa agaaaagctt aaatatagaa
     1081 cagatccagc agatcatccc gcacaggcat ccgttttgcc tgatcgacag gatcgaagat
     1141 tatgaaccgg gggaatttgc cataggctat aaagcggtaa catatacgga agaatttttt
     1201 aaaggccatt tcccgaataa accggttatg ccgggggtac ttattgtaga agcgcttgca
     1261 caaacaggag cagtggcgat tttaagtacg ccggaaaata aaggaaagat tgcatttttc
     1321 ggaggaatac aaaactgccg gtttaaagga atggtatttc ctggcgacgt gctgagatta
     1381 gagacaaaga ttattcgtca aaaaggtccg gtaggaatcg gaaaagcggt tgcaagtgtg
     1441 gacggtaaag tagtagtaag cgcagaattg acgtttatgg caggagaata gatttatgat
     1501 cgaaaaaata ttgatcgcaa accgtggcga gattgcggtt cggattattc gggcatgcag
     1561 agaaatggga attgaaactg tggcagtcta ttcggaggct gacaaggagg ctcttcatac
     1621 ccagcttgcg gatgaagcag tctgtatcgg tccggcgcag gcatcggaaa gttatctgaa
     1681 tatgcagaat ttgatcagcg cagcgctcgt ttcgggagca gatgcgattc atcccggatt
     1741 cggattttta tcggaaaatg caaaatttgc agaactttgc gaacagtgcg agattacctt
     1801 tatcggtcct gattctaaag tgatcggtaa gatgggaaat aaaacagaag caagagcaac
     1861 aatgatcgcg gcagggattc cggttatccg ggggacaaac ggagcggttt tagatctgga
     1921 agaagggaaa aaagaagcgg cgcgcatcgg atatcctgtg atcattaaag ccgccttggg
     1981 aggcggagga aaagggatgc ggacagcgca aagtgaagaa gagtttgaaa gttgttttgt
     2041 gacggcgcgc aaagaggcgg aaaaggcatt cggagacgga acaatgtata tcgagcattt
     2101 tgtggagcat cctcgtcata ttgaatttca aatccttgca gattcatatg gaaatatagt
     2161 tcatctcgga gaacgggatt gttcgataca gagaaatcat cagaagatga tcgaagagtg
     2221 tccgtgcgcc gcgttgtcag atgaacttcg taaaaaaatg ggcgaaacgg cggtaaaggc
     2281 cgcaagggcc gcaggctata caaacgccgg aacagtagag tttctgctgg aaaaagacgg
     2341 tacattttat tttatggaaa tgaacacaag gatacaggtg gaacatccgg taacggagtg
     2401 cgtaacaggg atcgatctga tcaaagagca gatacggatc gcaaacggcg gggaacttac
     2461 tttcaggcag gaagatatca ggatgacagg tcatgcgatc gaatgtcgga tcaatgcgga
     2521 gataccggag cgcaatttta tgccgtcagc cggaagaatc acagatctgc atcttccggg
     2581 aggaaacggg atccgtgtag atacggctgt ttacagcgga tgcgaggtgc tgccttatta
     2641 tgattccatg ttggcgaaac tgatcgttca cgggaaagac cgtcgagagg cggtagataa
     2701 gatgaggagc gctctgggag aaattattat agaaggaatc ggaacgaatg tcgattacca
     2761 gtatgagatt tcggaaaacg aggattttat atcaggaaag acagatgtgg attttattcg
     2821 gaggctgagt agtgaaactg cataatatgt ttaaaaagac cgtagggaaa gaacatggaa
     2881 catcgaaaaa tacaaaagta agaaatgttc ccgaaggatt gctgaaaaag tgcaataagt
     2941 gcggcgcggc tattttggca gaggatgccg taaataacaa ttatatttgt ccgaagtgtc
     3001 acggatattt caggatgcat gcaggacggc ggatcgagat gatcgcagat cccggaactt
     3061 ttgaagaatg ggatgcaaag atgaagtttg aaaatcctct ggaatttagc ggttatgagg
     3121 aaaaagtacg aagattaagg gaacagaccg gacttgacga agcggtcgtt acagggaaag
     3181 ttatgatcgc gggccatccg gcggtgatcg gagtatgtga cggacgtttt atgatggcga
     3241 gcatgggcga agtggtcggt gagaagatca caagagcgat agaacgggct acgaaagaaa
     3301 atcttccggt tattttgttc gcatgttctg gaggcgcaag gatgcaggaa gggatcgttt
     3361 ctcttatgca gatggcgaaa acggcggctg cgctgaaacg gcacagtgaa aaagggcttc
     3421 tgtatgtgag cgttttaaca gacccgacga caggaggagt aacggcaagc tttgccatgc
     3481 ttggagatat tattctggca gagccgaatg cgctgatcgg gtttgcagga ccgagggtga
     3541 ttgagcagac gatcggaaag aagctgccga aaggatttca gaggtcggag tttttgctcg
     3601 aacatggatt tgtagatcgg atcgtagagc ggtcggaaat gaaaagcgtt cttgcgcgta
     3661 ttttgcatat gcatgaccgt acaaaagaaa aaacagaaga aagtaccggg gaaaccgaaa
     3721 agattgaaag cactacacaa gaagatacta tgccaaaaca ggtgcggtca aaaggcggaa
     3781 taagcgcgtg ggaaagagtt cttctttcaa gaaaaacaga ccgtccggtc ggaacagact
     3841 atgtgaatat gctgttttct gattttatgg aatttcatgg agacaggctt tttagagacg
     3901 atcctgcagt ggcaggagga attgcaatgt tcgggaacat tccggtcacg gtgatcgctc
     3961 aggtgaaggg aaagacgacg aaagaaaatg tgacacgcaa ttttgcgatg atgtctccgg
     4021 aaggatatcg gaaaacatta cgattgatcc ggcaggcaga aaagttcgga cgtccggtga
     4081 tctgtatcgt agatactccg ggagcatttt gcggacagga agcagaagag cacggacagg
     4141 gagaagcgat cgcttcaaac ttatatgaac tgtcctcgat aaaagttccg atcgtttcgg
     4201 tactgatcgg ggaaggcgga agcggaggag cactggctct ggctgtggca gatgaagtgt
     4261 ggattctgga aaactctgtc tattcggttc tttctccgga aggatttgca agtatactct
     4321 ggaaagacag caagcgtgcg tcagaagcgg cggaagtgat gaaactgacg gcggatgatc
     4381 tgaaatgctt agggatagtg gaacaggtga tcagagaacc gaagtcgtat acgtcacaga
     4441 caatggctgg ggtatgtgaa gaattaaaaa acatgctcag cgtttttttg aaaaaatacg
     4501 gatctatgga tgaaagacaa ctgctgaatc atcgatatga cagatttcgt aaaatgtaaa
     4561 aagtgcttct tttccgaaca aagtgtgtgc tataatatag gttacgaaaa tgcaggcgca
     4621 agaagcgtcg gttatggaac acaatatatg gaaaaggagg aggtccgatg gatgcataca
     4681 gaacaattaa tgatacgctt gtaaatctgt ttaatgaaat atgggaatta gaaaaagaag
     4741 cgatcataac cgaagaattt aaagatatta caaataatga tatgcatatc annnnnnnnn
     4801 nagctaaaaa gctgaagatc acggcggggt ctctcacaac tgcggtcaat gcgcttgtca
     4861 gaaaggcata tgtgaagagg gaaagaagtg aagaggacag acgggtcgta tatatagcgc
     4921 ttacggaaaa aggagagaaa gctttttatc atcacgagca gttccatcgt cagatgacca
     4981 acgccgtgat agaaaagctg gatgacgaag aaattgacgc acttgtaaaa atgcttaaag
     5041 atatttctgc atttttcaga agttatcagt cataacacag gaaaacggaa atctgataag
     5101 gcgggatttg tggtatggat gaatttattt ttattttgga attgatcgga acagtagcgt
     5161 ttgcttcttc gggagcaatg atcgccatag aaaaaaagat ggatattttc ggggtaaatg
     5221 tgctcggggc gacaactgcg gtaggtggcg ggatcatgcg ggatatcata ctcggattga
     5281 cgccgccggg ggcgttttcg catccggtct atgttcttgt ggcggcgctg acatcgacga
     5341 ttctttttgt gatcgcatat gcgaaaccga cggcttttga gtctcgggta aagacagatt
     5401 attatgataa gctgatgttc tggtgcgata cggcaggact tggaatattt acagtagtgg
     5461 ggatacaggc ggcggtgcgg gccgtcggcg gggaaaatgt gtttttcttt gtatttatcg
     5521 gaacgcttac aggagtagga ggaggggtgc ttcgtgatat tatggcaggc gagactccgt
     5581 atattcttgt aaagcatatt tacgcatgcg ctgcgattgc gggaggaatc gtctgtgtag
     5641 tcggaagaac ggcattcgga gaagcttacg gtacgatatt aggactggcg gcaacggtgc
     5701 tgctccgttt tttggcagct catttcaggt ggaatctgat gcgtgtatca taaggttttt
     5761 catctcggaa aatagccgga tgataagaaa attacagtga aggcggactg aaattacagc
     5821 aagaatgata aaaatttgac gttttctgaa aaatgaggta caatccccta tgaaactttt
     5881 tgatttgggt tgaataagtg atactgaaca ggattagaga gcggtatcgg aaatacttgg
     5941 ttgtattatt ttaggaggga acaagtatgg cagtaaagaa aacaacttcg acatcaaggg
     6001 cagcggctaa aatagcaaat gcgatcaaag cggagcctgc aaaggcggct aaaacagcgg
     6061 cgaaagcagt aaaggaagaa gagaaaacag ctgtgaaggc agaggaaaag gcagaagtga
     6121 aagcttctga aaaagctcct gtaaaagcag agccggaaaa gaaagaagca gcgaaagcga
     6181 ttgagacaaa gaaagacaca acgaaaaagc ttgagacaaa agcggcagtt ttggtagata
     6241 caccggagac agaagagaag gcagctgtaa aaaaatcagt aaaagctccg gcaaaggcag
     6301 agaaaaaagc gccggtgaaa aaggcggcat ctaagaaagc ggaactgaaa acagagatgt
     6361 ttcttcagtt tgcgggaaaa gaatatacag acaaagagat ttttgacaaa ataaaatatg
     6421 tttggtcaaa agttttgaaa aataaagtcg gcgatatgaa agatgttaag atctatctga
     6481 agccggaaga gtcagcagct tattatgtaa ttaacggcga tacgacaggt aaagtcgatc
     6541 tgtaaaagtc tgctatcctc tctgtagcgg gattgccccg gcagggaggt tttttattta
     6601 tatgaggaga aaggaaggcg tatggcatga gcgaaaatac gaaaagtaag atgacggaga
     6661 agaaagaaca aaggaaacgg cttgaacagc agatccgttt tattattgaa gcggacaaag
     6721 tgaaaagcgt ttttcggcag acatatcttg ccgacggaag ccggaaagaa aacgatgcgg
     6781 aacattcatg gcatctggca ttgtctgcga tacttttgaa agagcatatg gacgcgcctg
     6841 ttgatctgat gaaagtgatc gtcatggtac tgatccatga tcttgtcgag atagatgcgg
     6901 gtgatactta tgcatacgat tccgagggca ccaagtcaaa aagagagagg gagttaaaag
     6961 cggcagagcg tattttcggt attcttccaa aagatcaggg aacatatttc agaagtttgt
     7021 gggatgaatt tgaagaatat agtacagagg atgcaaaatt cgctcatttg ctcgataatt
     7081 tccagccgct tttattaaat gacgcatcag acgggaaaag ctgggcagag catggagtgc
     7141 ataaggctca ggtgtgtaag cgaaatgagc ggattgcgga tacgtcggat atcgtatggg
     7201 aaaaaatgct tgaaattatg gataaacatg ttgagaaagg aaatttgaaa aatgtatgag
     7261 cgggaaacag agcggctgca aaagatgatt gaagaaagtg aaagtatcgt tttcttcggc
     7321 ggagcgggag tttcgacaga aagcggaata ccggatttta gaagtgcgga tgggatttat
     7381 catcagaagt atcgtttttc acctgaacag gttgtaagtc atacgttttt tatgaggtat
     7441 cctgaagcat tttatgaatt ttataaagaa aaaatgatga ttcttgatgc aaagccgaat
     7501 gcggctcacc tgaaattggc agaactggaa cgggcgggaa aacttacggc tgtcgttacg
     7561 cagaatatag acggtctgca tcagttggca ggaagtaagc gagtgtatga actgcatggc
     7621 agcattcaca gaaattattg tatgaactgc gggaaatttt atgatgcaca atatgtcaaa
     7681 aaaagcgaag gtgttccgaa atgctcctgc gggggcgtcg tgaaaccaga tgttgtcctt
     7741 tatgaagaag gtcttgatga ggagacgatc cggggcgctg tggaggcgat cgcgtctgca
     7801 gatatgctgc ttatcggagg cacgtctctc gtagtttatc cggcagcagg atttatcgat
     7861 tatttcagag gaagccgtct tgcggtgatc aataaatcag aaacggcaaa aaatatccga
     7921 tcagaactga cgatttcagc tccgatcgga gaaatattaa gccggataaa agtgtgatca
     7981 gtcagattcc catcgtccgg atgcgccgca tgggaggaca agtcccgaat cagtgacagg
     8041 aagtccgatt tcctgcgact ctacatggcc gttccatggc tttagttctg tcgagatcat
     8101 atacgtaagt acagcgggtg caagccctgt cgtataggaa ttgacaagga aaaaaagggc
     8161 gtctttagac aagatctttg tgcaaagttt gatgagcggg tggatcatat cttcgatctt
     8221 ccatatttcg cctttcggac cgcggccata agacggagga tccatgatga tcgcgtcata
     8281 agtattgccg cggcggatct cccgttcgac aaattttaca caatcatcga caagccagcg
     8341 gatcggagcg tctttcagcc cggaagacac tgcgttttct ttcgcccagc cgaccatgcc
     8401 ttttgaggcg tccacatggg tgacatgcgc tccggcggcc gctgccgcaa gagttgcgcc
     8461 gcctgtatag gcaaaaagat tcaaaacttt taccgggcgt cccgcgtttc ggatcttttc
     8521 cgaaaaccaa tcccagtttg tcgcctgttc gggaaagagt ccggtatgtt taaaactaaa
     8581 tggtttcaaa ttaaatgtaa gagatccata gtgaatctgc cactgctgag gcagactgaa
     8641 aaattcccat tccccgccgc cttttttact gcggtggtaa tggccgttca tctgtttcca
     8701 tcctttatga ttctttttcg tgttccagat tacctgagga tcggggcgaa caagaagata
     8761 atctccccat cgttccagtt tttctccgtt agaacaatct ataacttcat aatctttcca
     8821 gttgtctgca atctcataat aatatccttt ctccatacag tgtgtgcgaa tatagctata
     8881 gtttaccaca agcatgtaca acaggaaagc ataaaacaat ctaaattgta acagttcagc
     8941 catgcagcgc ggttttacga atgcacaggc tgagctttta ctgtctgtgg atttcgtcaa
     9001 cttgaatttt caaagtgaaa tacggcatga ttcatgatac aatataaaat aagaaataaa
     9061 attttgaggg agatgtctta tggtgaaaat gacagtagat gcaatgggag atcactgtcc
     9121 gattccggta gtgaaaacaa aaaaagcgct tggaaaatta cagggagcag gacagataga
     9181 agtgcttgta gataacgaga cggcaatgaa aaatgtcatg aagatggcaa aaagcagcgg
     9241 cgcgtcagcg gaatcggaaa agatcagcga tcgggaatac aaagtgatga ttacggtaga
     9301 agagaagaac gcggctgtat cagaaagcga tcaaaaagga cagaccgcag gaaaagaaag
     9361 ccgggcgcag gcagaaggcg gggggaaaga agcaggcgaa tcggatcaca atccggcagc
     9421 agaaaatgga agtaacagta gtgcgcaggg gatgaacaaa gtgcagttat catctgcggg
     9481 atgcgcgaca tgtatcggaa cggtcgttgc ggtttcttct gataaaatgg gaagcggaaa
     9541 tgatgagctg ggcgcagttt taatgaagag tttctttttt gcggagacgc agcttgatac
     9601 gcttccggat aaaatccttt tttacaacgg aggcgcgaaa cttacggtgg aaggttcgga
     9661 ctgcctagaa gatatcagga ttttggaaaa agaaggcgtc gagatactga cgtgtggcac
     9721 atgtcttgac ttttacggat taaaggaaaa actggcagtc ggagcagtga caaatatgta
     9781 tacgattgca gaaattcttc aaaacgcagt cagcgttgtt tctccgtaac gacaggagaa
     9841 ggacataagg aaaaatattt attatgggtg aaaaagaaag aatggcaaaa agtataaacg
     9901 gatagataaa agtgtaagga cagtattgca aaagtttgat acaatacgat aacagctcat
     9961 aggaaagcgg catgttttgt cccgaaatgc agcttttgag ttgagtaagt ttacatgggg
    10021 aaaaggaaaa caaaaataca gatatcccct acataataag gaggtattaa gacaaatgaa
    10081 aaagaccaaa aaactggtaa tgtcatggct acttgccttt tgctgtgtca tcactttggt
    10141 tgcgccgtct gtcgtaaatg cggcagcaga tgatttcgag atcatcgatg atacagacgc
    10201 acgttttgta tattcagacg gtaatgcaaa taacggcgga tgggaatcgg cgggagcggg
    10261 cgacgcttcc gttacagagc actggagcaa cacggcaggc gcgacattgg acattacatt
    10321 caacggaaca aaactggagc tttacggtaa aaaagctgca aatcatgcaa tgttcagcgt
    10381 gagcattgac ggaagcgagg cagtagaatg cgacgcctat gccgcagtga cagaaccgga
    10441 ggcaaaattg tttgaaagca aaacgcttgc ggcaggaaat catacagctc atatcacagt
    10501 tttagaaaaa agaaatgaag cttctacagg gacaggaagc gtttatggcg tacagtttgt
    10561 gtatgcaaaa gcatttgagg cagagattgc cgagccggaa tttccgggat atacagaagt
    10621 tgacgatgct gtttttacaa caaatcatga gcccttcaag atccagtacg agccggcagg
    10681 cgcttggact gcgggaagcg gcaacggaaa tttgttttat aacggaacag agcattacag
    10741 cgcaaaaggc gttgatgtgt cttatgagat gatctttact ggaaccggag tagagatcca
    10801 tgccagcaaa aatacagttc atatgaactg cgacgtttat atcgacgatg tgaaagtcgg
    10861 agaactggcg gcacaaaatg ccggagcaat gcacaaacag aaattatttg agaaaaaaga
    10921 tcttgcgaat acaaagcata cgcttcgtgt cgttccgaaa gagggagaaa gcattgaagg
    10981 caaagtaata cagcttgata agattgttgt ttttcacgat gaaattactg tgcctgattc
    11041 gatcgtattg aaccggacgg agctgacgat gacacccggc gcttcatcag agcttacggc
    11101 gtctgtcatt ccgtggaatg caaatgcgaa gcttgtgtgg acaagcagcg acagcagtgt
    11161 tgtagaagta aacgatggaa aactgactgc aaaagagatc gatgtgaaaa agacagttac
    11221 tgtcacagcg gcaagcgctg aagacaacag tgttttagca gaagctaaga ttacagtcga
    11281 tccggctctg gcatttatga acgcatatgt aggcaatgaa aaactcttag agacagagct
    11341 tgattatgat aagctgaaag caggaaacgg aagcgtttat aacggaacag cctggttgaa
    11401 tgatgaactg aattctaaaa ttgtcgtgac gacagaaaaa gacgttcaca atgttcaggt
    11461 tacagcaagc gatttcaaga atgaaaaggg acaggttttg tcaaaagaca atattgatat
    11521 taaatggctc aaagaaattg cggcaaaaga aggacgtaac gcacaggggc agacaaaaaa
    11581 ttatccggat gtgatctaca aaggcgggaa gaaagatatc gatgcacaag atgtacagtt
    11641 tgcatgggta aatatcgcga ttccgaaaga tacagcggca ggaaattata caggtacgat
    11701 tacggtttcg gcggacgaat tagacaagcc gttcgagctt acgtacaaca tcgaagtgct
    11761 gaaccttgta cagccggcgc cggaggcaac agaactgcaa gtttggcagc atccgttttc
    11821 agttgcgaat tattatttag gtttaggtga aaatccgtcg ggcggcatca caaacgaggt
    11881 gcgggaagat ttctatttta cagaaaaaca ttttaatttg atgagagact cgatcaaaga
    11941 gtatgtttcg atcggaggtc atgacgtagt tgccaatgtc gtagaagagg catggaatca
    12001 tcagtcatat tataacgatc tttcgatggt aaaatggacg aagaaagcgg acggaacatg
    12061 ggaatttgat tatgattggt atgatgcatg gatcaatttc atgatcgagt gcaaagtttt
    12121 agatcctgca aacggaatcg gacagatcaa gtgttacagt atcgttccat ggaacaatca
    12181 gatcgcttac tatgatgaag cgcagggtaa agtagtgaaa gaatcacaca atccgggaac
    12241 tgcaaagtgg aaagagatgt gggaatcgtt cttaaaagat tttatggaac attccaaaaa
    12301 aatgggatgg tttgacatca cttatatttc aatggacgaa agagggctcg atcagcttga
    12361 accagcggtt gagatgatcg aatcagtgaa agacgaagat ggaaaccact ttaagatctc
    12421 ttcggcgctg aactatgcag ctccggaata ttatgagttt acagaccgta tcgatgatat
    12481 ttcgatcaat ctcggaaaca caggaaacgt acagcagatg aacgatctgt cagatcaccg
    12541 cagagactta ggtctgacga caacgatgta cacatgtacg ggagattatc cgagtaactt
    12601 tatgatcagt gatccgggcg acaactattg ggatatctgg tacacgatga cattaggaac
    12661 agacggctat atgcgttggg catgggataa ctatgtatac gatatgcacg gcgatgcaac
    12721 ttaccgttac tgggagccgg gcgacggatg gttcatttat ccgatggaaa gagaagcggt
    12781 aggagaggac tttaacgctt ccttctacag cacaccgcgt tatgaattgt tcaaacaagg
    12841 tatccgtgat gtggcaaaag caaaatatct gttgaacagt gaaagcgcga cagcagaaga
    12901 aaaaacagaa ttgacagatg ttgtagagca tttggcaaaa ccgcagaaag gaacatatca
    12961 aggttctgcg gtggcggcat ccgaaaaaga ccgtatgctc gttcattctg aaacagagcg
    13021 cgctttagat gcgacaaacg cgctggcaag aaacgttgcg gagagggaga acccgaatcc
    13081 gaagccggaa agcgcagata aaacagcgct taatgcggcg attaaagatg cagaagcttt
    13141 gaaaaaagaa gattacacag cagaaagctg gaaagcgttt gagacggcgt tgaatgcggc
    13201 aaaagaaaca gcggctgata aagatgcagc tcagacagaa gtagacaacg cattaaacgt
    13261 attgaatgcg gcagttgcta aacttgagaa ggtgaaagat ccgaatcagc agcagccggt
    13321 tcaaccacag ccggagcaga caaaaccgga taagacgact cctcagacag gagatcggac
    13381 aaacgcagcg cttctgtttg gatgcgcagt tctttcggga gcaggtgtgt tctttgcata
    13441 tcgcagacgc agaacaataa aataatcgtt ttctaaagta gatttcggaa aacagaggaa
    13501 aagaacgaaa gagtcggaga cggatctttc gttctttttg taacggtgga gggatgaagt
    13561 ccgaaagatt cttttttggt ctgctttaga aatctacaaa acagttgtta aacaggtaga
    13621 tttgagtcat aataaaaacg actattatgg attttttcgg aggaagaaga gatgaggaag
    13681 atggagaaga ttcatttaac acaatttgca aaacacggcg gctgtgctgc aaaaatcggt
    13741 cctgatacgc ttgggaaagt gcttgggagg cttccgaagt ttcatgagga caacctgctt
    13801 gtcggttttg aaacttcgga tgacgcggcc gtatataagc tttcggacga tacggcagtg
    13861 atacagacac tggatttttt tacgcctgta gtagacgatc cctatacgtt cggacagatt
    13921 gcggctgcga atgcgctaag cgacgtatat gcgatgggag gagaaccgaa gatcgcgttg
    13981 aatatcgtct gttttcccgg ggaactggat ccggattatt taggggaaat tctgcaagga
    14041 ggcgcggaaa aggttcttga atcgggagct gtccttgttg gaggacattc gatacaagat
    14101 gacgttccga aatacggact gtcggttatg ggacttgttc atccgaatcg catctataaa
    14161 aacttcggat gtaaaaaagg agatgttctg atccttacga aacaactggg aagcggaatc
    14221 gtcaatacgg cggtcaaagc acagatggct tcggaagctg ccgggaaaga agcggttgcc
    14281 gtgatgacgc agttaaacaa aaaggcaagg gaaatactgg acaagtttac gatccatgca
    14341 tgtacagacg ttaccgggtt cgggcttctc ggacattgcg gggaaatggc ttcggcgagc
    14401 agagcgaaga tagagctttg gccaaaagat atcgcctata ttgaaggggc aaaggaatat
    14461 gcgcagatgg gacttattcc cgggggagca tatcgcaaca gaaatcatgt cgcacagctt
    14521 cttgaagcgg gaaataccga tgagatgtat gtagatctgc tcagtgaccc gcagacttcc
    14581 ggcggtttgc tggcggcagt tccggaagaa gaaaaagatg cgattttgga agaatttgat
    14641 aaagcacaga tggaaacaaa agtgtcggtt gtcggaaaag taatagactc cgaatgtgaa
    14701 gaagcaaaaa tatatatccg ttaatatagg aggagagaca aaatgattta tctggataat
    14761 gccgctacga ctttgcacaa accaaaggaa gtacaggaag ctgttatagc ggcgtttggg
    14821 cagatgggaa attccggaag aggggcatcg gagccggcgc ttaaggcatt gggaacagtc
    14881 tacgatgcaa gagaagcgct ggcacggctt tttcatgccg aaaatgcgag tgagattgtg
    14941 tttaccgcaa attctacaga ggcgctgaat attgcaatca agggaatact tgatccggga
    15001 gatcatgtca tcacaacggt gctggaacat aattccgtgc tgcgcccgct gtatgaatgc
    15061 cgcaggaaag gtgtggagat gacaattctt acatgcgacg aatatgctaa catttcatat
    15121 gaagagatgg aaaaggcagt cagaaaaaat acacgggcga tcatatgtac tcatgcgtca
    15181 aatatgaccg gaaatatgat ctctttggag agtgtcggaa agattgcggc aaagtatggt
    15241 attctcttta ttgttgacgc ttcccaaacc gcgggagtat gggagatcga tgtacaaaaa
    15301 tatgggattt ccgtcctttg ttttaccgga cataaaggac tgatgggacc gcagggaaca
    15361 ggtggtatgt atgtaaaaag cggggtaaag atccgtccgc ttttgtcagg cggcagcggg
    15421 atagattctt ataatatgca tcatccgtcg cagatgccgg aagcgcttga agccgggacg
    15481 ctgaacggtc acgggatcgc gggacttcgg gcggcattgt catttttgga aagagaaggg
    15541 atggaacgcc tgcggaaaag agaactggag ctgatgcgcc gtttttataa cggagtcgct
    15601 gaaattccga atgtgaaagt gtacggagac tttcggacgg acagaagggc agccatcgtt
    15661 tctttgaata tcggcgatta tgattcggca gagataagcg atgaattaag cggcagatat
    15721 gggatcgtta cgcggtccgg agcgcattgt gcgccgttga tgcatgaagc gcttggaaca
    15781 aagatacagg gtgcggtcag attcagtttt tcccattata atacggaaga ggaaattgac
    15841 gaggcaatcc gcgccgtaaa agagcttgct gaggaataag aagaacaggc gcggggcaag
    15901 gtgttatgga atcataagaa acgggggaga agatgaaaaa acgagagaaa aaaattgtga
    15961 tcacttttca tacgacaacg gatgcgatgg caatggaacg gtactgtaaa aagagaaaag
    16021 aaccgggcag actgattcct gttccgggta cgatctcggc cggatgcgga cttgcatggt
    16081 gcgttctgcc gcaagagagg gaacatcttt tgaaagtgat ggaggagggg gatataactc
    16141 cgcaggctgt gcatgaatgt atgttataaa atatcacagt tctaccacaa aagattcaca
    16201 taggtttgat atcgtcttaa acaaatatat tgtatgaaat atgtggaaat aagattaaag
    16261 tatattgaaa aagtatatgt cacagataga ggggagtgca gaaatccaca gaaagaatac
    16321 aaagttagga ggatatcatt ttgatagaag taaaaaattt ggtaaaaaaa tatggaaatc
    16381 atttagccgt agatcacctg gactttcaga ttaagccggg aatggtttac ggttttttgg
    16441 gtcccaacgg agcaggaaaa tctactacaa tgaacattat gacaggatgc atgggggcaa
    16501 ctcaggggga agtgctgatt aatggatatg acatcttgaa agaacctgaa aaagccaaaa
    16561 gatatatcgg atatttgccg gaacatccac ctctttatat ggatatgact gtgcgggaat
    16621 atttggattt tgcagcagag ctgaaaggaa ttagaaaatc tcaacgaaga gaagctgttg
    16681 atgaagcaga aaaaatggta aaggttcagg atgtggaaca tcgcttgatt cgaaatcttt
    16741 caaaaggcta tcgtcagaga gtagggctgg cacaggcaat tttaggattt ccggatatga
    16801 ttattcttga tgaaccaagc gttggattgg atccgaaaca gattatagag atgagagatt
    16861 taatccgtaa gttggctgag aaccatacgg ttattttaag ctcgcatata ttgacggaga
    16921 tcagagaagt atgtgattat attctgatta tttctaaagg aaagctggta gcacaggata
    16981 cgctggaaaa cttggagaag atgatagggt ttcacgatgt gattgaatta gaaacatcgg
    17041 cttcggagga ggaaattcat agaatactgg ggaggattcc cggagtttgt gcgatgcaga
    17101 tgcgtgtgaa aggagaaaag tgtacttatg tacaggtaaa agtgactgga gataaaagca
    17161 gtcagaataa cagaaaagta agagaagaga ttttctgtga atttgcgaaa gagaaaaaaa
    17221 cattatattc attgtcggcg cctgaaacga gtttggaaga agcgtttatg aaattgacac
    17281 agaacgataa tatagaaaga aatatgcaaa tcgaagaatc gcttgtgaaa gatagacagg
    17341 taaaggacgg acgggtgaaa gaaagccgac cgggagagaa acagatagga gtggaaacca
    17401 gaaaatgtga gatgaataca gagggaggaa aagaggatga aggcggttta taaaaaagaa
    17461 ttaaaagcgt attttcagtc tataataggg tgtgtattta ttgcctttct tattgcattt
    17521 actggaattt atttcatggc gtataatatg acaacaggat atccgtattt ttcttataca
    17581 ctttcaggat cgttgatcgt atttattgta gggattcctt tgattacgat gcgttcattt
    17641 gcagaagaga gaaagaataa aacagatcag attctgttga cagcgccagt gagtttgtgg
    17701 aaagttgtgg ttggaaaata cctggcgatg gtgacggtaa ttgcaatccc gaatcttata
    17761 ttttgtattt tccctcttat cattaaattg caggggacgg cgtatttgac agttgattat
    17821 atttcgattt tgatgttctt cctgctcggc tgtgtttttg cagctatagg aatgctttta
    17881 tcagcgctta cagaaagtca aattattgcg tatataggga cgtttggtat ttttctgatt
    17941 ctatatttat gggatggtat cttatctctg tttccaaata cggcgatgag cggacttgtg
    18001 tttataattt tgcttatttt gctggcagct ttctacatat ggagaatgac gaacagtttt
    18061 tcgattgcgg gaggagtggg ctgtgtcgga gtggcagtag cgatagcagt gtattttctg
    18121 aaagagacgc tttatgaaaa tctgtttaca gagtctttgg gcgggttcgc tctgacaaat
    18181 atttttaccg atattacaca aaatagtatt gtagatgtga gcggaattgt attgtacttg
    18241 tcgatgatca cattattttt atttttgaca gttcagtcga ttagaagacg actttgggaa
    18301 agtgcggcaa aaaaacatgg agcgtatagc gtgacgatga cagcggtttt tcttgcaatt
    18361 acagttgtca taaacttgat tgcatgccag ataccggaaa aattccgaaa gatagatgtg
    18421 agtaatacaa aaatttacga gatttcagat acgacagaag atttcctgaa agaaatggat
    18481 aaagaa
//
LOCUS       DBMDXF010000013        18280 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_938_length_18280_cov_2.083503, whole genome shotgun sequence.
ACCESSION   DBMDXF010000013 DBMDXF010000000
VERSION     DBMDXF010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 18280)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18280)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18280
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_18280_cov_2.083503"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /gene="leuS"
                     /locus_tag="ACZPD4_01140"
     CDS             complement(<1..1722)
                     /gene="leuS"
                     /locus_tag="ACZPD4_01140"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1377717.1"
                     /translation="MGTKIMYNHKAIEKKWREKWAENSVNPRVDDNGNKKQKYYCLDM
                     FPYPSGNGLHVGHWRGYVISDVWSRYKLQQGYYIVHPMGWDAFGLPAENYAIKMGVHP
                     AKSTAENVANIKRQINEIAALYDWDMEVNTTDPNFYKWTQWIFVKMFKEGLAYEKEFP
                     INWCPSCKTGLANEEVVNGKCERCGAEVTKKNLRQWMLKITAYADRLLNDLDKLDWPE
                     KVKKMQADWIGKSYGAEVEFPVDGRDEKITVYTTRPDTLYGATFMVLAPEHEMAASLA
                     TDETREAVEKYIYDASMKSNVDRLQDKEKTGVFTGSYAINPLSGAKTPIWLSDYVLAD
                     YGTGAIMCVPAHDDRDFEFAKKFNIPIIQVIAKDGKEIENMTEAYTEASGTMINSGEW
                     NGMESSVLKKEAPHIIEERGFGKATVNFKLRDWVFSRQRYWGEPIPIIHCPDCGNVPV
                     PEEELPLTLPDVDSYEPTGTGESPLAGIEEWVNCTCPVCGKPAKRETNTMPQWAGSSW
                     YFLRYVDSKNDKELVSREKADEMLPVDMYIGGEEHAVLHLLYSRFYTKFLYDIGVVDF
                     DEPFHKLF"
     misc_binding    complement(1786..2038)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2134..3450)
                     /locus_tag="ACZPD4_01145"
     CDS             complement(2134..3450)
                     /locus_tag="ACZPD4_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR1377718.1"
                     /translation="MRETENNKRYKAAMQGVGMQNVKMQQEAADKGSLQVNVTSSVNS
                     YPISEARISISYTGVPENTLEQLVTDSSGQTDMIELDAPPEEWSLDPENERQPYSEYT
                     LNVSAPGFEPVSIAGTEILANTKAIQNIRMRPTDTGREEEEIFVIPAHTLYAEYPPKI
                     AEDEIKPVNESGEIVLSRVVVPEYIVVHDGSPRDSTAKNYYVKYKDYIKNVASSEIYA
                     TWPANTIRANVLAIMSFTLNRVYTEWYRNKGYDFTITSSTAFDHKWIPERNVYESISV
                     IVDELFANYLSRPNVRQPILTQYCDGKMVSCPNWMTQWGSKELGDKGLTPIEILRYFY
                     GDDMYINIAPEISGIPSSWPGYVLEIGSSGEKVRQIQEQLNVIAGAYPAIPKITADGI
                     YGSKTAESVRKFQEVFQLPQTGKVDYSTWYKISEIYVGVSRIAELT"
     gene            complement(3551..4582)
                     /gene="rseP"
                     /locus_tag="ACZPD4_01150"
     CDS             complement(3551..4582)
                     /gene="rseP"
                     /locus_tag="ACZPD4_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1377719.1"
                     /translation="MGIIIAVLLFSFIIIFHELGHFLLAKKNGIRVDEFSLGLGPTLL
                     GKQIGETKFSLKLLPFGGACMMGEDDADDLSEGSFNSKSVWARMSVVAAGPIFNLILA
                     CVFCFILIMITGYRSPEITGVLDGYSAQEEGLQAGDVITEINGRNVHIWDDVSLYTMT
                     HADEAPFKVEYERDGKKYTAKLEPRQLEGDAAPLLGVTSGDIVKPGIFKSVEYSIYKV
                     KYWMNYTVDSLRMLVTGQAGLKDLSGPVGIVNAVDDMYQEAAPAGFGVVMLSMMNFGV
                     LLTTNLGILNLLPLPALDGGRLVFLIIEAIRKKRVPPEKEGMVHFAGFALLMVLMAVV
                     MYNDIMKLF"
     gene            complement(4586..5728)
                     /gene="dxr"
                     /locus_tag="ACZPD4_01155"
     CDS             complement(4586..5728)
                     /gene="dxr"
                     /locus_tag="ACZPD4_01155"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1377720.1"
                     /translation="MKRIAVLGSTGSIGTQTLEIVRTNKDIKITALAAGNNIELLEKQ
                     IREFKPSIAAVWNEEKAAELKIKTADLDVEILSGMDGLLAAASWKDSQIVVTAIVGMI
                     GILPTIEAIKAGKDIALANKETLVTAGHIIMPLAEQYKVSILPVDSEHSAIFQSLQGG
                     QRKALHKILLTASGGPFRGKKREELTGIQVEDALKHPNWEMGQKITIDSSTMVNKGLE
                     VIEAKWLFDVSVDQIQVVVQPQSIIHSMVEYEDGAVIAQLGTPDMKLPIQYALYYPER
                     RYLPGDRLDFSTLSQITFEKPDMETFYGLKFAYEAGRKGGSLPTVFNAANERAVAMFL
                     GRQIAYLEIPEIISACMENHKNIADPTVEEILKTERETYEFIKNRW"
     gene            complement(5810..6622)
                     /locus_tag="ACZPD4_01160"
     CDS             complement(5810..6622)
                     /locus_tag="ACZPD4_01160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1377721.1"
                     /translation="MFKTRLLSGIMLMIIALTTVIAGGQVLFTVLFAISLIGMSELYK
                     VFGIEKKAPGIVGYIFAFGYYALIYMEEYLPGEKHTWFMLLFMAYLICQMAVLVFSYP
                     KYNTQQIMAAFFGVFYVAVMLSYIYLTRMLPGGVFTVWLVFICSWGCDTCAYCVGMLI
                     GKHKMAPVLSPKKSVEGGIGGIAGAALIGVLYALAVNHWGNAGASIVSYAVIGAVGGA
                     ISQIGDLAASAIKRYHNIKDYGKLIPGHGGILDRFDSVIFTAPIIYYLAVLL"
     gene            complement(6626..7369)
                     /locus_tag="ACZPD4_01165"
     CDS             complement(6626..7369)
                     /locus_tag="ACZPD4_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR1377722.1"
                     /translation="MVSPLSYREEEKMNVPQHIAIILDGNGRWAKAKGMPRNYGHAQG
                     SKNVEKICEEAWRMGIKYLTVYAFSTENWSRPENEVAALMKLLRNYMKTCLKTAAKND
                     MKIRVIGDIEPLDDDIKSRIRELEAATTDNGGLNFTIALNYGSRDELTRAAQKMAKDC
                     AEGKIKAEEIDESVFETYLDTHGIPDPDMMIRTSGEQRLSNYLLWQLAYSEFYFTDVP
                     WPDFTKEELVKAIEEYNHRHRRFGKVEEA"
     gene            complement(7408..7959)
                     /gene="frr"
                     /locus_tag="ACZPD4_01170"
     CDS             complement(7408..7959)
                     /gene="frr"
                     /locus_tag="ACZPD4_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1377723.1"
                     /translation="MNERLTTYDSKMTKTINNLDGELATIRAGRANPHVLDKLTVDYY
                     GSPTPIQQVANVSVPEARMIQIQPWEKSMLKEIEKAILMSDLGINPTNDGSTVRLVFP
                     ELTEDRRKELAKDVKKKGEAAKVAVRNIRRDGNVAFKKLKGTEVSEDEIKDLEDDLQK
                     ITDKYIEKIDKMVEVKTKEIMTV"
     gene            complement(7992..8687)
                     /gene="pyrH"
                     /locus_tag="ACZPD4_01175"
     CDS             complement(7992..8687)
                     /gene="pyrH"
                     /locus_tag="ACZPD4_01175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1377724.1"
                     /translation="MKRVLLKLSGEALAGDKKTGFDEATCIKVAEQVKKLTEDGIQVA
                     IVTGGGNFWRGRTSETIDRTKADQIGMLATVMNCIYVSDIFRYVGMKTEVFTPFVCGS
                     FTSLFSKDAAVAALEEGKVIFFAGGTGHPYFSTDTGAVLRAIEIEADAMLLAKAIDGI
                     YDSDPKVNPEAVKYDEISIQEVIDQKLAAVDLTASILCLENKMPMLVFGLNEENSIVE
                     TMSGNFTGTKVTV"
     gene            complement(8740..10308)
                     /locus_tag="ACZPD4_01180"
     CDS             complement(8740..10308)
                     /locus_tag="ACZPD4_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HIR1377725.1"
                     /translation="MKRNTSHTIKRAQRFPVILIGEGLLVGAVGGLIVLLYRIALTCA
                     GKWLGEALAFIKGNPLRIAGWFAVLMLLAVLVGKLIKWEPMISGSGIPQVEGEILGKL
                     NQNWLKVLPAKFAGGFLCLFGGLSLGREGPSIQLGVMSGQGISRILSRGKTEERYLMT
                     CGASAGLAAAFHAPLAGVMFALEELHKGFSTSLLISVMTASVTADYISSHIMGLDPVF
                     RFQLEQTLPQNYYWMILVLGVILGLMGALYNKVMLKAQELYKRPKFLNETTRLMIAFS
                     AAGVLGLCMPSVLGSGGDLIVALTDKEMVLKVAIITFLVKFLFSAVSFGSGAPGGIFF
                     PLLILGAMVGGIFSMTGTEIFGLDPMYMNNFVLLAMAGFFTAIVRAPITGIILLFEMS
                     GSVSQMLSLALVSVTAYVTATLVRSEPIYESLLANLLQREKEEEQPVYAGGQKVLSEF
                     VIMGGSALENRTVMEIDLPDNCLLVAVDRGGKEFIPKGKTCLMAGDKLTVMADERDAG
                     YVHDKMKNLCYTSF"
     gene            complement(10392..11072)
                     /locus_tag="ACZPD4_01185"
     CDS             complement(10392..11072)
                     /locus_tag="ACZPD4_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1377726.1"
                     /translation="MRELCSSPYFGIALSVLAFGIGVKLNEKLKTPVCNPLVIAIVLI
                     VGVLLIFKIPYEDYNAGGKIINMFLAPATACLAVAIYTKIQILKQYWLPILVGCTAGS
                     LASMGSVYGLCRLFGLDKSLTVSLLPKSVTTPIAVSISEPAGGVVPITVAAVIGTGIL
                     GGIFAPLLIKLFKVSDPVAAGLAIGASSHAVGTSKAIEIGEVEGAMSGLAIGICGIVT
                     VIVSIFLV"
     gene            complement(11069..11458)
                     /locus_tag="ACZPD4_01190"
     CDS             complement(11069..11458)
                     /locus_tag="ACZPD4_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1377727.1"
                     /translation="MKIIRQIGIIFTVCWLSLVIEKLLPFTFPASVIGMILLFLCLFT
                     GVLKIEHIQEKADFLLGNMAFFFVPAGVSIMNYFDVLKHSIVQLVFVCIISTIITFAV
                     TAWSVKLTLRLMKSRKPENLQSGGGEK"
     gene            11774..12787
                     /gene="aroF"
                     /locus_tag="ACZPD4_01195"
     CDS             11774..12787
                     /gene="aroF"
                     /locus_tag="ACZPD4_01195"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1377728.1"
                     /translation="MIIVLKPHSTEETITRVENMVKNRGLDTHIVRGTEMTIIGCIGD
                     TTLIDPRLFEVDSSVDKVMHVQEPYKLANRAFHPEDSVIDVSGVKIGGGHMGLIAGPC
                     SVESFDQVLEIAKAAKAAGANLLRGGAFKPRTSPYSFQGLGLDGLEILCQVKQEVGLP
                     IVTELMSPEYLDVFNEKVDLIQIGARNMQNFDLLKQLGQVDRPILLKRGLNATYEEWI
                     MSAEYIMASGNENVILCERGIRTFETYTRNTLDLQSIPVLRKKTHLPVVIDPSHAGGK
                     WWLVEPMAKAAVAAGADGLMIEVHNNPECALCDGAQSLKPEKYDTLLKEIKLIAEVVG
                     KQL"
     gene            12799..13860
                     /gene="aroB"
                     /locus_tag="ACZPD4_01200"
     CDS             12799..13860
                     /gene="aroB"
                     /locus_tag="ACZPD4_01200"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR1377729.1"
                     /translation="MKLTVNLKENSYPIYIENGILNRAGEYIADLFHGQRIMIVSDDN
                     VFPLYGKQLCDSLSGYECHSLVLPHGEPTKSFDTLPKVYSAMLDAKLTRSDLVIALGG
                     GVIGDLTGFAASTYLRGIKCVQIPTSLLAQVDSSVGGKTAVDLPQGKNLVGAFFHPKM
                     VLIDPLVLNTLPDHFIHDGMGEVIKYGCIKDSGLFDLLLSHSSFEDLKPDLPKIIARC
                     VDIKRIVVEADQFDTGERMLLNFGHTLAHTIEQYYQYERESHGEAVGIGMYQLTKMSE
                     KRGLTAKGCAEKIRKALDIYNLPTSCKLTVSELIKAISLDKKNLNGHLNLVLLKEIGD
                     SYIYPTKIGFFENELNTFM"
     gene            complement(14051..16330)
                     /locus_tag="ACZPD4_01205"
     CDS             complement(14051..16330)
                     /locus_tag="ACZPD4_01205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR1377730.1"
                     /translation="MRVVNKGIRKVDAKALVTGRAVYTNDIASSDSLIVKLVRSPHAH
                     ALIKEINTTRAEALPGIECVLTYKDCPNKRFTLAGQTYPEPSPYDRLILDQRVRFVGD
                     AVAIVAGTSEAVVKQAMRLIKVKYEVLEPVLDFRTAKDHPILVHPEENWRSLCPVGAD
                     NKRNLCAHDVSESGDVDAVLASCDHVIDRVYHTKANQQAMMETFRTYTYMDAYGRLNI
                     VSSTQVPFHVRRILGNALDIPKHKIRVIKPRIGGGFGAKQTSVSEIYPALVTWKTGKP
                     AKIIYTREESLIASSPRHEMEIHVRLGADKEGNIKGIDLYTLSNTGAFGEHGPTTVGL
                     SGHKSIPLYGSAEAFRFSYDVVYTNVMSAGAYRGYGATQGIFALESAVNELAQTLHMD
                     PTVLRAKNMVREGQMMPAYYGETTRSCALDRCLERAKEMIGWDEKYPCYDCGNGKVRG
                     VGVAMAMQGSGISGVDTGAVEIKVNDDGFYSLMIGATDMGTGCDTILTQMAAECLECE
                     MEDIVVHGVDTDNSPYDCGSYASSTTYVTGMAVVKTCETLRKKICERGAEYLGCSPDD
                     VEFDGEKVVNLKTKEEITRKEIGNKVMCSSNAPLSATEAFSSPVSPPPFMVGIAEVEI
                     DKETGVVEPIDYAAVVDCGTVVNPNLARVQTEGGIAQGIGMALYEDIIYSEKGKNYSN
                     SFMQYKIPSRLDVGEIRVEFESSYEPTGPFGAKSIGEIVINTPSPAISNAVQNAVGVI
                     VRELPITAEKVYKGMAEKE"
     gene            complement(16393..16872)
                     /locus_tag="ACZPD4_01210"
     CDS             complement(16393..16872)
                     /locus_tag="ACZPD4_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1377731.1"
                     /translation="MEIQFVLNDKQVKAEVGADTILLNVLRDLGCKSVKCGCETTNCG
                     LCTVWINGKSRLSCSVLAASIEGESVTTLEGVEKEAAQFGEFLADEGAEQCGFCSPGF
                     IMNVLAMARELQNPTLDEIKEYLAGNLCRCTGYMGQLRAIKKYIEVKNAEKKGGKEE"
     gene            complement(16872..17675)
                     /locus_tag="ACZPD4_01215"
     CDS             complement(16872..17675)
                     /locus_tag="ACZPD4_01215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR1377732.1"
                     /translation="MLTIGNYVKAKTPEEAYELNQARSSRIMGGMMWMRLSNARVKTL
                     IDISGLGLDQIEETEDEIRIGAMCTLRQIEQSEAVKKLCGDGIEEAVRHIVGVQFRNQ
                     ATVGGSIYGRFGFSDVLTAFLALDTYVELYDGGRICLAEFAKKKPDNDLLLFIVVKKK
                     KRQFRYDSIRQTKTDFPVIACSVSREKANGQETWYFAVGARPMKADLIEKKWSVAKDA
                     SLEKIEEYAALAAAEFKYGTNMRGSAGYRQHLAKVLIKREIMSILSEEI"
     gene            17847..>18280
                     /locus_tag="ACZPD4_01220"
     CDS             17847..>18280
                     /locus_tag="ACZPD4_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1377733.1"
                     /translation="MIPYKQLALAEVFEDCQNKFDNDKYQFLSLLDQTINLDEIVPVS
                     FVTHFHASTGRPRKHPLYPMIKALLIQRIFSIPTDTLLIIFLKYSQELRDFCGFRVVP
                     DASKFTRFKQDFLMDLQSMFDHLVDITEPICQRIDSNLASMS"
BASE COUNT         4831 a         4745 c         3264 g         5440 t
ORIGIN      
        1 gaacaatttg tggaacggtt cgtcaaaatc tactactccg atatcataca aaaattttgt
       61 ataaaaccga gaatacagaa ggtgaagcac ggcatgttct tcgcctccga tatacatatc
      121 taccggcagc atctcgtctg ctttttctct ggacactaat tctttatcgt ttttactgtc
      181 tacataacgc aagaaatacc atgaagatcc tgcccattga ggcatcgtat ttgtctcacg
      241 ctttgcaggt tttccacata ccggacatgt acaatttacc cattcttcaa ttcctgcaag
      301 cggtgattct cctgttcctg tcggctcata ggaatctacg tccggaagcg taagcggaag
      361 ttcctcttcc ggtaccggaa cattaccgca gtcaggacag tgaatgatcg gaatcggttc
      421 tccccagtaa cgctgacgtg aaaataccca gtcacgcagt ttaaagttga ctgttgcttt
      481 tccgaatccg cgctcttcta taatgtgcgg agcttctttt ttaagcactg acgattccat
      541 tccattccac tcgcccgagt tgatcattgt tcccgaagct tctgtataag cttctgtcat
      601 attttcgatt tcttttccgt cttttgcaat tacctgaata ataggaatat tgaatttttt
      661 tgcaaactca aagtcacgat catcatgagc aggaacacac ataatcgctc ctgtaccgta
      721 atcagccaat acataatcag acagccagat cggtgtcttc gctccgctaa gcggattgat
      781 tgcatagctt cccgtaaata cgcctgtctt ctctttgtcc tgaagacgat ctacgtttga
      841 tttcattgac gcatcgtaaa tatatttttc cactgcttct cttgtctcat ctgtcgcaag
      901 agatgccgcc atctcatgtt caggagcaag caccataaat gtcgcgccgt aaagcgtatc
      961 aggtcttgtc gtataaacag tgatcttctc atctcttccg tcaacaggga actccacttc
     1021 tgctccgtag gattttccga tccagtcagc ctgcatcttc tttacttttt ccggccagtc
     1081 cagcttatcg agatcattta acagccgatc tgcatatgct gtgatcttca acatccactg
     1141 acgaagattt ttcttcgtga cttccgctcc gcagcgctcg catttgccat ttacaacttc
     1201 ttcatttgcc aaaccggtct tacaggacgg acaccagttg atcggaaatt ctttttcata
     1261 tgcaagtcct tctttaaaca tcttcacgaa aatccactgc gtccatttat aaaagttcgg
     1321 atctgtcgtg ttgacttcca tgtcccaatc ataaagcgct gcgatctcat tgatctgacg
     1381 tttaatattc gccacatttt ccgccgttga tttcgccgga tgtactccca tcttgatcgc
     1441 atagttttca gcaggcagac cgaatgcatc ccatcccatc ggatgtacaa tataatatcc
     1501 ctgctgaagt ttataacggc tccatacgtc agaaattaca taccctctcc aatgtccgac
     1561 atgaagaccg tttcctgacg gatacggaaa catgtcaaga cagtaatact tctgtttttt
     1621 atttccgtta tcatccactc ttggatttac cgaattttcc gcccattttt cacgccactt
     1681 tttttcaatc gctttgtgat tatacattat ctttgtaccc atatgtttct ctccttcatt
     1741 catcgttata ttacgcataa aagaaagcct tccgttcctg aaaactcaag agacgaaaag
     1801 gcttatttcc gtggtaccac tcttgttcat ccgaccggac cgggaaaact tttttgttcc
     1861 tgtatcctca tccgaccaaa tgcactcatt cattctataa cgggaattcc cgctttcgtc
     1921 tacttgaagt tcaacgaaag aactccgagg tgagttcaca gtttacgttt ctgcctcgca
     1981 ccgaccggca gttctctgaa tacgatacac tcctactatt cctcttccat gtttttgcaa
     2041 tatccttatt cattataaat atgtcattaa aataagtcaa ggtattttca ccggaactat
     2101 gccgattccg ctatcacttt cgatttttca aagttacgtc aactctgcga ttctggatac
     2161 gccgacataa atttcggata ttttatacca tgtactgtag tcgactttcc ccgtttgcgg
     2221 cagttgaaac acttcctgaa attttctgac cgattccgcc gtttttgaac cgtaaatacc
     2281 gtctgccgta attttaggta tcgcaggata cgctcccgca attacattca actgctcctg
     2341 tatctgcctc accttttctc cgcttgaccc gatctccaat acatatcccg gccacgaaga
     2401 tggaatacct gatatttccg gcgcaatatt aatatacatg tcatctccgt aaaaataacg
     2461 aagaatctcg atcggtgtca aacctttatc tccaagttcc ttcgaccccc attgtgtcat
     2521 ccaattcgga cagctcacca ttttcccgtc acaatactgt gtcaatatcg gctgccgcac
     2581 gttcggtctt gaaagataat ttgcaaacaa ttcatctacg atcacggaaa tgctttcata
     2641 tacatttctt tccggtatcc acttatgatc gaatgctgta gatgaagtga tcgtaaaatc
     2701 ataccctttg tttctatacc attcggtata aacgcgattg agcgtaaaag acataatcgc
     2761 aagaacgttt gcccgaatcg tatttgcggg ccaggtggca taaatttcgc tggacgccac
     2821 atttttgata taatctttat acttcacata atagtttttt gcagtcgaat ctctcggaga
     2881 accgtcatga acaacaatgt attcaggaac aacaacacgg cttaaaacga tctctccgct
     2941 ttcatttaca ggcttgatct catcttccgc tattttagga ggatattctg catataaagt
     3001 atgagcaggg atgacgaaaa tttcttcctc ttccctgccc gtatctgtcg gtctcatcct
     3061 gatattttga attgcttttg tatttgccag aatttctgtt cccgcaatgc tcacaggctc
     3121 aaaccccgga gcgcttacat tcaatgtata ttccgaataa ggctgccgtt cgttttcagg
     3181 gtccagactc cactcttccg gcggcgcatc cagctcaatc atatccgtct gccccgagga
     3241 atcagtcacc aactgttcta atgtattttc cggaacaccg gtatacgaaa tcgaaatgcg
     3301 agcttccgaa atcggatatg aattcaccga tgaagttaca ttcacctgta atgatccttt
     3361 atctgccgcc tcctgctgca ttttcacatt ctgcatcccg actccttgca ttgcagcttt
     3421 atacctctta ttgttttctg tttcccgcat acgttctcct tcatcagatt tattctctct
     3481 aaatatatat gcggaagctt tcgtttagaa caactttcgt attcggagtc tctgcaagag
     3541 catatgataa tcaaaacaac ttcatgatat cgttatacat cacaacggcc atcagtacca
     3601 taagcaatgc gaatcccgca aaatgtacca ttccttcttt ttccggcgga acacgttttt
     3661 tacggatcgc ttctattata agaaaaacaa gtcgtcctcc gtccaatgca gggagcggaa
     3721 gcagatttaa aattccgaga tttgttgtca gcagaacacc gaagttcatc atactgagca
     3781 tcacaacgcc gaaccctgcc ggagctgctt cctgatacat gtcgtctacg gcatttacaa
     3841 ttcctaccgg acctgaaaga tctttaagtc ctgcctgacc tgtcacaagc atgcgcaaac
     3901 tgtctactgt ataattcatc cagtacttta ctttgtaaat actgtattca acagatttga
     3961 aaatccccgg ttttactata tctccgcttg tcaccccaag cagcggtgcg gcatcgcctt
     4021 ccaactgtcg aggctccagt tttgccgtat atttcttgcc gtcacgttca tactctactt
     4081 tgaaaggcgc ttcatcggca tgtgtcattg tatacagact gacatcatcc catatatgca
     4141 cgttccgccc gttgatctct gtgataacgt ctccggcttg aagtccctct tcctgcgcgg
     4201 aatagccgtc cagcactcct gttatttccg gacttctgta accggttatc ataatcaata
     4261 taaaacagaa aacacacgca agaatgagat tgaagatcgg tcccgccgca acaactgaca
     4321 ttctcgccca cacggattta ctgttgaaac ttccttccga cagatcgtcg gcatcatctt
     4381 cgcccatcat acacgcgccg ccgaaaggaa gcagtttcag agaaaatttt gtctctccga
     4441 tctgttttcc taataacgta ggtccaagtc ctaaagaaaa ttcatctacc cgaattccgt
     4501 ttttctttgc aagaagaaaa tgtcccaatt catgaaagat gataataaaa ctaaataata
     4561 aaactgcaat aatgattccc attcgctacc acctgttttt tataaattca tatgtttccc
     4621 gctcagtctt tagtatctct tccactgtcg gatctgcaat atttttatga ttttccatac
     4681 acgccgaaat aatctccgga atctcaagat atgcgatctg tcttccgaga aacattgcaa
     4741 cagcacgctc gtttgccgca ttaaacacag tcggaagaga accgcctttt ctcccggctt
     4801 catacgcaaa cttcagtccg taaaacgtct ccatatccgg cttctcaaaa gtaatctgcg
     4861 aaagtgtgga aaaatcaaga cgatctcccg gaagatacct tctctccgga taataaagcg
     4921 catattggat cggcagtttc atgtccggtg ttccaagttg agcgatcacc gcaccgtcct
     4981 catattctac catagaatga ataatgcttt gaggctgtac gacaacttgg atctgatcga
     5041 ccgaaacgtc aaaaagccat ttggcttcta tcacttcaag acctttattc accattgtag
     5101 aagaatcgat cgtaatcttc tgtcccatct cccagtttgg atgtttcaaa gcatcttcaa
     5161 cttgtatacc ggtaagttct tctctttttt tcccgcggaa cggtccgccc gaagcagtca
     5221 ataatatttt atggagagct ttgcgctgtc ccccctgaag cgactgaaaa atcgcgctgt
     5281 gttcactgtc tacaggaagg atcgacactt tatattgttc cgccagcggc atgataatat
     5341 gccctgccgt cacaagtgtt tctttatttg ccagcgcaat atcctttccg gctttgatcg
     5401 cctcgatcgt cggcaggatt ccgatcatac ctacaatcgc agttacaaca atctgtgaat
     5461 ctttccaaga tgcagcggca agcaatccgt ccatcccgga taagatctcc acatccagat
     5521 ccgcagtctt tattttcagc tctgcggctt tttcctcatt ccacactgcg gcaatcgacg
     5581 gtttgaattc ccggatctgt ttttccagaa gttcaatgtt atttcccgca gcaagcgccg
     5641 tgatttttat atctttgttt gttcttacaa tttcaagcgt ctgggttccg atcgagccgg
     5701 tagaccctaa gacagcgatt cttttcatat ctttgtacct cacttctgca tctgtcctgt
     5761 aacatatcgc ccgtaaactt atcttccggc ttcataatgc ccttatgtct tacaataaaa
     5821 ccgcaagata atatatgatc ggagcagtaa aaatgacact gtcaaaacga tccagaattc
     5881 ctccatgacc cggaatcagt ttcccgtaat ctttaatatt atgataacgt ttgatcgccg
     5941 atgccgccag atcgccgatc tgggaaattg cgccgccgac tgcaccgatc actgcataag
     6001 aaacgatact cgcccctgca tttccccagt ggttcacagc taatgcataa agcacaccaa
     6061 tcagggctgc gcctgcaatt cctcctatcc cgccttctac ggattttttc ggactcagaa
     6121 ccggagccat cttatgcttt ccgatgagca tgcctacaca gtatgcacat gtatcgcatc
     6181 cccacgaaca gataaacaca agccatacgg taaacactcc tcccggaagc attcttgtaa
     6241 ggtaaatgta agacagcatt accgctacat aaaatactcc gaaaaatgcc gccatgatct
     6301 gctgtgtatt atatttcgga taagaaaata caagcactgc catctggcag atcaaatacg
     6361 ccataaaaag cagcataaac catgtatgtt tttctcccgg gagatattct tccatataaa
     6421 taagcgcata atacccgaaa gcaaaaatat aaccgacaat tcccggcgct tttttctcaa
     6481 tgccaaacac tttataaagt tctgacattc cgatcaggct gatcgcgaaa agaaccgtaa
     6541 ataaaacttg ccctccggca atcactgtcg tcaatgcaat gatcatcagc ataatgccgc
     6601 tcaacaaccg tgttttaaac atagattacg cctcctcaac tttgccgaat cttctgtgtc
     6661 tgtggttata ttcttcgatt gcttttacaa gttcttcttt tgtaaaatcc ggccagggca
     6721 catccgtaaa ataaaattcc gaatatgcaa gctgccaaag aagataattc gacaatctct
     6781 gctccccact cgttcggatc atcatatctg gatccggaat cccatgagta tctaaatatg
     6841 tctcaaacac cgattcatca atctcttctg cttttatctt tccttctgca cagtctttcg
     6901 ccattttttg cgctgcccgt gtcagctcat ctctgcttcc gtaattcaag gcaatcgtaa
     6961 aattcagacc gccgttgtct gtggtagcag cttctagctc gcgaatcctg cttttgatat
     7021 cgtcatccaa cggctcaata tctccgatca cacggatctt catatcattc ttggccgctg
     7081 ttttcaagca tgttttcata taattacgaa gcagtttcat aagagccgca acttcatttt
     7141 ccgggcggct ccaattttct gttgaaaatg cataaaccgt cagatattta attcccatac
     7201 gccatgcctc ttcacagatt ttctccacat ttttgcttcc ctgtgcgtgt ccgtagtttc
     7261 gcggcatccc ctttgctttc gcccatctgc catttccgtc taaaataatt gctatatgct
     7321 gcggtacgtt catcttttct tcctctctat acgaaagggg gcttacaatc aaagccccca
     7381 gtttttgtaa cggcgttaag aatccgatta cacagtcata atctcttttg tctttacctc
     7441 gaccatctta tcaatctttt cgatatattt atccgtaatc ttctgaagat cgtcttccag
     7501 atctttgatc tcatcttccg aaacttccgt tccttttaac ttcttaaacg ctacatttcc
     7561 gtcacgacgg atatttctga cagccacttt cgcagcctct ccttttttct tcacatcttt
     7621 tgccagttct tttctccgat cctccgtaag ttcagggaat acgaggcgaa ccgtcgaacc
     7681 gtcattagtc ggattgatgc cgagatctga cataaggatc gctttctcaa tctctttgag
     7741 catgctcttt tcccacggct ggatctgaat catccttgct tccgggacag atacgtttgc
     7801 cacctgttgg atcggtgtcg gcgatccgta ataatcaact gtcaatttat caagaacatg
     7861 tggatttgcg cgaccggctc tgatcgtagc taattctccg tcaagattat tgatcgtttt
     7921 tgtcatcttt gaatcatatg tcgtcaatct ttcattcata tttatatcct cctgcaaatt
     7981 ttttataacg cttatacagt cactttcgtt cccgtaaagt ttccgctcat cgtttccaca
     8041 atactgtttt cctcgttcaa accgaataca agcatcggca tcttattttc aaggcagaga
     8101 atcgatgctg taagatccac cgcggcaagt ttctgatcga tcacttcctg tatggatatc
     8161 tcgtcatatt tgaccgcttc cggattcact ttcggatcgc tgtcataaat accgtctatc
     8221 gcctttgcaa gcagcatcgc gtccgcttca atctcgatcg ctcttaatac cgcccctgta
     8281 tctgttgaaa aatacggatg tcccgttcct cctgcgaaaa agatcacttt tccttcttcc
     8341 agcgccgcta ctgccgcatc ttttgagaat aatgacgtaa acgaaccgca tacaaacggc
     8401 gtaaacactt ctgttttcat cccgacataa cggaaaatat cagatacata aatacagttc
     8461 atcaccgttg ccagcattcc gatctgatct gcttttgtcc ggtcgatcgt ctcgctcgtc
     8521 cttcccctcc agaagttgcc tcctccggtc acgatcgcca cctgaatgcc atcctcagtc
     8581 agtttcttta cctgctctgc aactttaata caggttgcct cgtcaaaacc tgttttttta
     8641 tctcccgcga gcgcttctcc gctcagcttt aacagtactc ttttcatatc gtactctcct
     8701 ttgttttctt cacattaact atttaaaatc aatgctgacc taaaatgaag tataacataa
     8761 gtttttcatt ttgtcatgta catatcccgc atctcgttca tctgccataa ccgtcagttt
     8821 atcgcctgcc ataaggcatg tttttccttt cggaataaac tcttttccgc ctctgtctac
     8881 cgcgacaaga aggcagttgt ccggcagatc tatttccatg acggtgcggt tttcaagagc
     8941 agaacctccc atgatcacaa actcgctcag cactttctgc ccgcccgcat aaaccggctg
     9001 ttcttcctct ttttctcttt gcagcagatt tgccagaagg ctttcataaa tcggttcaga
     9061 tcttaccaaa gttgcagtga catatgcagt caccgaaaca agtgcaagag aaagcatctg
     9121 acttaccgag ccggacatct caaacagaag tatgatccct gtgatcggcg cacgaacgat
     9181 cgccgtgaaa aatccggcca ttgccagtaa aacgaaatta ttcatataca tcggatctaa
     9241 accgaagatt tccgttcctg tcatagagaa gattccgccg accattgcgc ctaatatcag
     9301 aagcggaaaa aaaattccgc caggagcgcc ggatccgaaa ctgactgccg aaaacagaaa
     9361 tttcacaaga aacgtaatga tcgcaacttt caacaccatc tctttatccg tcaaagcaac
     9421 gatcagatcc cctccgcttc caagtaccga aggcatgcaa agtccaagca ctcccgccgc
     9481 cgaaaacgcg atcataagac gagtcgtctc gtttaaaaat ttcggtcttt tataaagttc
     9541 ctgcgctttt agcattacct tattatagag cgctcccatc agacctaaaa tcactcccaa
     9601 cacaaggatc atccaataat aattctgggg aagcgtctgt tccaactgaa atcggaacac
     9661 cggatcaagc cccataatat gagacgatat ataatctgcc gtaaccgaag ctgtcataac
     9721 cgaaataagc agacttgtcg aaaacccttt gtgcagttct tccaacgcaa acataacgcc
     9781 tgccaaagga gcgtgaaatg ccgcagccag tccggcgctc gccccacacg tcatcaaata
     9841 ccgttcttct gttttccctc tgcttaaaat ccgtgatatt ccctgtccgc tcatcactcc
     9901 aagctgtata gacggacctt ccctgccaag agacagtccg ccgaacagac aaagaaatcc
     9961 tcccgcaaac ttagcgggca gaactttaag ccagttttga ttcaattttc caagtatttc
    10021 accttcaacc tgcggtattc cgctccccga tatcatcggt tcccatttta tcagtttccc
    10081 gacaagcact gcaagcagca tcaaaacagc aaaccatccc gcaatccgca gtggatttcc
    10141 ttttataaac gccagcgctt caccaagcca ttttcccgca caagtaagcg cgattctgta
    10201 aaggagcacg atcaaccctc cgacagctcc tacgagaagt ccttccccga tcaatataac
    10261 aggaaaacgc tgcgctcttt ttattgtatg cgatgtattt cttttcattt tatcctcatt
    10321 tcttccattc cttttttcta cagttccgac aaaaagccgt aaagatcatc gaagatcttc
    10381 ccgaaaatat ttcacaccag aaatatagac acaatcacag tgacaatacc gcatataccg
    10441 attgcaagtc cgctcatcgc gccttccact tccccgatct caatcgcttt cgatgttcct
    10501 accgcatggc tggatgcccc tatggcaagt cctgccgcaa ccggatcaga taccttaaat
    10561 aattttataa gaaggggcgc aaatatcccc cctagtattc ctgttccgat aacggcggca
    10621 accgtaatcg gaacgacgcc gcctgccggt tctgatatgc tcacggcaat cggggttgtt
    10681 acggatttcg gcagcaaaga aactgtcaga cttttatcca gtccgaataa ccggcacagt
    10741 ccgtaaacac ttcccataga tgccaaagat ccggccgtgc agccgacaag aatcggaagc
    10801 cagtactgtt tgagtatctg tatttttgta tagattgcca ctgcaaggca ggcagttgcc
    10861 ggagcgagga acatattaat aatctttcct cccgcattat aatcttcata gggtatttta
    10921 aaaataagaa gaaccccgac aataagaaca atggcaatca ccaatggatt acagacaggt
    10981 gtttttaact tttcatttaa tttcaccccg attccgaagg ctagaacgct tagcgcaatt
    11041 ccaaaatacg gtgaagaaca caattctctc atttttctcc gcctcctgac tgtagatttt
    11101 ctggtttcct gcttttcatc aaacggagtg tcaatttcac gctccatgca gtcactgcaa
    11161 atgtaataat tgtagaaata atacaaacaa aaactaactg tacaatcgaa tgtttcagca
    11221 catcaaaata attcataata cttacgcccg caggaacaaa aaagaacgcc atatttccga
    11281 gaagaaaatc cgccttttcc tgaatatgtt ctatctttaa aactccggta aacaggcata
    11341 aaaaaagaag tatcattccg atcacacttg ccggaaaggt aaaaggaagc agtttctcta
    11401 tcacaaggct aagccagcat actgtaaaaa tgatcccgat ctgtctaatg attttcattg
    11461 catcaactcc ttaattgttc ttgatcgcct atcattaact ctactaaaat accactgtct
    11521 ttgcaagact gtcaatgtcg ggcgctattc tttaaatctg ttcatatatc atttatctgc
    11581 acattaaaag gcgaaaaaag agatagaaat aaaagaaata tgcaaaaaaa atcattgaca
    11641 ttcttttttt tgtattctat gatactatta gtttcgcact ttaaacagtt aaaattttgc
    11701 agtatgaagt tgaacgcttt tgttcttccc ttcgctgccg aaaatatttt tcataataca
    11761 gggaggcatt tttatgatta tcgtactgaa accacattca acagaagaaa ctattacacg
    11821 tgtagaaaat atggtcaaaa accggggact cgacacacac atcgtccgcg gtacagagat
    11881 gacgatcatc ggatgtatcg gagatacaac gctgatcgac ccacgactct ttgaagttga
    11941 cagttccgta gataaagtta tgcatgttca ggaaccttac aaacttgcca accgcgcgtt
    12001 tcatcccgaa gactccgtga tcgacgtctc aggagtcaaa atcggcggag gacatatggg
    12061 attgatcgca ggtccttgtt ctgtagaatc ttttgatcaa gtgctcgaga tcgcaaaagc
    12121 cgcaaaagcc gccggagcta atcttctgcg cggaggagca tttaaaccga gaacaagccc
    12181 gtactcattt caagggttag gtcttgacgg actggagatt ctctgtcagg tcaaacaaga
    12241 agtcggtctt ccgatcgtaa cagagctgat gtctcctgaa tacctcgatg ttttcaatga
    12301 gaaagtagat cttattcaga tcggagcccg caatatgcag aattttgatt tattaaaaca
    12361 gctcggtcag gtagaccgtc cgatcttatt aaaacgcggt ttgaacgcta cctacgaaga
    12421 atggatcatg tcagccgaat atattatggc ttcaggaaat gaaaacgtta tcctgtgcga
    12481 gcgtggtatc cgtacatttg aaacatatac aagaaacact cttgacttac agagcattcc
    12541 tgtacttcgc aaaaagacac atcttcctgt tgtaatcgat cctagtcatg caggaggaaa
    12601 atggtggctt gtagaaccga tggcaaaagc tgccgtagcg gcaggcgcag acggtctgat
    12661 gatcgaagtt cacaataatc cggagtgtgc tctttgcgac ggagcacagt cactgaaacc
    12721 ggaaaaatat gacacacttc tcaaagaaat aaaactaatt gccgaggttg tcggaaaaca
    12781 gctataaaag gagattctat gaaactaaca gtcaatctga aggaaaactc ttatccgatc
    12841 tatattgaaa acgggatttt aaatcgcgcg ggagaataca tcgccgatct gtttcacgga
    12901 cagcgtatta tgatcgtctc cgatgacaat gtatttccac tgtacggaaa acaattatgc
    12961 gattcccttt ctggctacga gtgtcattct ctcgtcctcc ctcatggaga accgacaaaa
    13021 agtttcgaca cacttccgaa agtctactcc gcaatgcttg acgcaaaact gacccgaagc
    13081 gatcttgtga tcgctctcgg aggcggagtg atcggggatc tgaccggatt tgcggcatct
    13141 acctacttgc gaggaatcaa atgtgtgcaa ataccgactt ctcttttggc acaggtagac
    13201 tcatccgtcg gcggcaaaac cgccgtagac cttcctcagg ggaaaaatct tgtaggcgcg
    13261 ttcttccacc cgaaaatggt gctgatcgat cctcttgtac tgaacacact gcctgatcat
    13321 ttcatccatg acggcatggg cgaagtgatc aaatatggct gcatcaaaga ctccgggttg
    13381 tttgatcttc tcctttctca ttcttcattt gaagatctca aaccggatct gccaaagatc
    13441 attgccagat gtgttgatat caaacggatc gttgtagaag cagaccagtt tgataccgga
    13501 gagcggatgc ttttaaattt cggtcataca ctggcccata cgattgaaca atattatcaa
    13561 tatgagcggg aaagccatgg agaagccgtc ggaatcggca tgtatcagct tacgaaaatg
    13621 tcagaaaaac gcggccttac cgcaaaggga tgcgctgaaa agattcggaa agctttagat
    13681 atctacaatc ttccgacaag ctgcaaattg actgtttccg aactgatcaa agcgatctcc
    13741 cttgataaga aaaacttaaa cggacactta aacctcgtcc ttttaaaaga aatcggcgac
    13801 agttatattt atcctacaaa aatcggtttt tttgaaaacg agttgaacac atttatgtga
    13861 gaatgttgta cctatagtct tcataaacga ataaactttt acataacgac aaaaataaag
    13921 caggagatgc cgcaaatcaa cagcgcatct cctctatttt tatatctttt atatctttat
    13981 tttttatatc tttgttttta atattttttt aatatctcca ttttatatac ttgcttttac
    14041 atacttgctt ttattctttt tccgccattc ctttatacac tttctccgcc gtgatcggaa
    14101 gctccctcac aattactccg actgcatttt gaactgcatt tgaaattgca ggagacggag
    14161 tattgataac gatctctcca atggattttg cgccgaaagg tcctgtcggt tcataactgc
    14221 tctcgaattc cacacggatc tctccgacat caagacggct cggtatttta tactgcataa
    14281 aggaattact gtagttcttt cctttttcgc tgtaaatgat atcctcataa agcgccattc
    14341 cgattccctg ggcgattccc ccttcagtct gcactcttgc aagattcgga ttaacgaccg
    14401 ttccgcagtc tacaacagcg gcataatcga ttggttcaac aactcccgtc tctttatcga
    14461 tctccacttc tgcaataccg accatgaacg gcggcggaga tacaggagaa gaaaaggctt
    14521 ctgttgcgga cagcggcgcg ttgctgctgc acattacttt gtttcctatt tcttttctcg
    14581 ttatctcttc ttttgttttc agattgacaa ctttttctcc gtcaaactcc acatcatcag
    14641 gactgcatcc gagatattcc gcccctcttt cgcatatctt tttccgaagt gtctcacacg
    14701 ttttcacaac tgccattccc gtaacatatg tcgtactgga tgcgtaagat ccgcagtcat
    14761 acggggaatt atctgtatct actccatgca cgacaatatc ttccatctca cactcaagac
    14821 attctgcagc catctgtgtc agtatcgtat cgcagcccgt tcccatatcg gtcgccccga
    14881 tcattaaact gtaaaatccg tcatcgttta ctttaatttc aaccgcgcct gtatctactc
    14941 ccgaaattcc cgatccctgc atcgccatag caacgccgac accgcggact tttccgtttc
    15001 cacagtcata acagggatat ttttcatccc atcctatcat ttctttcgca cgttcaagac
    15061 atctgtcgag agcacagctt ctcgtcgtct ccccatagta ggcaggcatc atctgacctt
    15121 cccgcaccat atttttcgct ctgagaacgg tcggatccat atgaagtgtc tgtgccagtt
    15181 catttaccgc agactctaag gcaaaaatac cttgtgtcgc cccatatccc ctataagctc
    15241 ccgcagacat aacatttgta tacactacgt cataactgaa ccgaaacgct tctgcgcttc
    15301 cgtaaagagg aatcgattta tgcccggata acccgaccgt tgtggggccg tgttcaccga
    15361 acgcgcctgt atttgacaat gtatacagat caatcccttt gatatttccc tctttatccg
    15421 ctcctaaacg aacatggatt tccatttcgt ggcgaggtga cgaagcgatc agagactctt
    15481 ctcttgtgta aataatcttt gccggttttc ctgttttcca tgtaacaagc gccggataaa
    15541 tctccgaaac cgacgtctgt tttgccccga atccgccgcc aatacgcggc tttataacac
    15601 ggattttatg cttcggaata tcaagcgcat ttccgagaat gcggcggaca tggaaaggca
    15661 cttgggtaga agatacaata ttcagccttc cgtatgcatc catatatgta tatgtacgaa
    15721 atgtttccat catcgcctgc tgatttgctt ttgtatggta tacccgatcg atcacatggt
    15781 cgcaagatgc aaggactgca tctacatctc cgctctcact tacgtcatga gcacacaaat
    15841 tacgtttgtt atccgctccg acaggacaaa gacttctcca gttttcttcc ggatgaacga
    15901 ggatcggatg atcttttgcc gttctgaaat ctaaaaccgg ctccaatact tcgtatttta
    15961 ctttgatcag cctcatagcc tgctttacaa ccgcttccga agttccggcg acgattgcga
    16021 ccgcatctcc tacgaaccgg actctctgat caagaataag ccggtcgtac ggacttggct
    16081 caggatatgt ctgacctgcc agcgtgaaac gtttattcgg acagtcttta tatgtaagca
    16141 cacattcaat tcccgggagc gcctccgcac gtgtagtatt aatctctttg atcagcgcat
    16201 gagcatgagg gcttcttaca agctttacga ttaagctgtc tgaagatgcg atatcatttg
    16261 tatatacggc gcgtcctgtc acgagcgctt ttgcgtcaac tttccggatt cctttattta
    16321 caacacgcat ctgccgcatc gtttctctta tttccttgtt ctcactgcac acattctgtc
    16381 cttctgccat gtctactcct cctttccgcc ttttttctct gcatttttta cttctatata
    16441 ttttttaatc gcgcgaagct gtcccatata tcccgtgcag cggcaaagat ttcccgcaag
    16501 gtattctttg atctcgtcca atgtcgggtt ctgaagttct cttgccattg cgagcacgtt
    16561 cataataaat cccggagaac agaaaccgca ctgctccgct ccttcatctg ccaaaaattc
    16621 tccgaactgc gcagcctctt tttcaacgcc ttccaaagtc gtaacgctct ctccctcgat
    16681 acttgcggca agaaccgagc aggaaaggcg ggattttccg ttgatccaga ctgtacacaa
    16741 tccacagttt gtcgtctcgc agccgcactt tacgctctta catccgagat ctctcaacac
    16801 atttaacaaa atcgtatctg cgccaacttc tgcttttacc tgtttgtcat ttaaaacgaa
    16861 ctgtatctcc attatatttc ctccgataaa atcgacatta tttctctctt gataaggacc
    16921 tttgccaaat gctgtctgta cccggcgctt ccgcgcatat ttgtaccgta cttaaattct
    16981 gccgctgcca aagccgcata ttcctcgatt ttctctaaag acgcatcttt tgcgacagac
    17041 catttctttt cgatcagatc tgccttcatc ggtcttgcgc ctaccgcaaa ataccatgtc
    17101 tcctgcccgt tcgctttctc tcttgacact gaacaggcga tcacagggaa atccgtcttc
    17161 gtctgacgga tggaatcata acggaactgt cttttcttct tcttaacaac tatgaaaaga
    17221 agcagatcat tatccggttt tttctttgca aattcagcta agcagattct tcctccgtca
    17281 taaagttcta catatgtatc cagtgccaaa aatgcagtta atacgtctga aaaaccgaat
    17341 cttccgtaaa tgcttcctcc aaccgtcgcc tgattccgaa attgaactcc gacaatgtga
    17401 cgcacagcct cctctattcc gtctccgcat aattttttaa ctgcttcgct ttgctcgatc
    17461 tgacgcagcg tacacattgc tccgattcgt atctcatctt ccgtttcttc tatttgatcc
    17521 aatcctaaac ctgaaatatc gatcaatgtt ttcactcttg catttgaaag gcgcatccac
    17581 atcattccgc ccatgatcct gctgctgcgc gcctgattta attcatatgc ttcttccggc
    17641 gttttcgcct ttacataatt tccaatcgta agcacaatcg ttctcctttc ctgatacata
    17701 cataacatag gtgtttgcga aacgccacaa gccgcataaa tacggcattt taacttttat
    17761 aaaacaaaac atctcctcaa cggattgtgg taaaatagag ttgcctagaa actataaacc
    17821 aaccacccga aaggagatgt acctatatga taccatataa gcagctcgct ttggcagaag
    17881 tttttgaaga ttgccaaaat aaattcgata atgacaaata tcagtttctt tctctacttg
    17941 accaaaccat taatcttgat gaaattgttc ctgtttcttt tgttactcat tttcacgctt
    18001 ctacaggaag acctcgtaag catccacttt atcctatgat taaggctctc cttattcagc
    18061 gcattttctc aatcccgaca gatactcttc tgattatatt tttaaaatat tctcaggaac
    18121 tacgggattt ctgtggcttc cgtgttgttc ctgatgcctc aaaatttacc cgctttaaac
    18181 aggactttct tatggactta caatcgatgt tcgatcactt agttgatatc actgaaccga
    18241 tctgccaacg tattgattct aaccttgctt ccatgagtat
//
LOCUS       DBMDXF010000014        16631 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1042_length_16631_cov_1.840048, whole genome shotgun sequence.
ACCESSION   DBMDXF010000014 DBMDXF010000000
VERSION     DBMDXF010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 16631)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16631)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16631
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1042_length_16631_cov_1.840048"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="ACZPD4_01225"
     CDS             <1..1195
                     /locus_tag="ACZPD4_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377734.1"
                     /translation="AELAQKYGFDLDPTKKVYNMSVSEKQTVEILKVLYKGADILILD
                     EPTAVLTPQETEKLFAVLKNMKKHGKSIIIITHKLNEVMAISDRVAILRKGEYIGVVE
                     TSETDPDQLTEMMVGRPISLEIERPEVKRGGKRLRVIDLTCLSGDGVKALDNVSFDAY
                     AGEILGIAGIAGSGQKELCETIAGLYPSVGGSVLYSESSENGAVKERILGLSPDEIAK
                     KGISMAFVPEDRLGMGLVAGMDMTDNVMLRSYKSNKGIFVDRKSPRDLAEKLIKELEI
                     VTPGVETPVGRLSGGNVQKVLLGREIACQPSVLIVAYPVRGLDINASYRIYDLLNEQK
                     KKGVAVVFIGEDLDVLMQLSDRIMVMCGGKVSGIVDPRKVTKEEIGLMMIRTEQDGEK
                     EEVTE"
     gene            1192..2289
                     /locus_tag="ACZPD4_01230"
     CDS             1192..2289
                     /locus_tag="ACZPD4_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377735.1"
                     /translation="MSSNVTHAKEPLFRMVKRDAIPQKKAWMIRAAAFLLSLATGAVV
                     IMMLGHNPFSVYISMIKGAWGTKTVSYETIKIAIPLLITAIGISFAFKMKFWNIGAEG
                     QILVGAVAAGAFGLFTAHIFPKIPLVILMMLAAIVAGGLYGLLPAVCKAKWGTNETLF
                     TLMLNYIALAFIKYLMNGPWKAPGSSYPKIAMLVPGARLTKVFGVHWGWILALILVVV
                     SYIYFNKTKQGYEIAVVGESNNTARYAGINVGRVFRRTMFLSGALCGLVGMLQFAGSD
                     FTITEGTAGGVGFTAITVAWLAKMNPAGMLVVSVFIAMLERGANAIQTQFKIPASAAD
                     LLIGIILFFMLGCEFFITYKLIGRGKEEHHA"
     gene            2282..3274
                     /locus_tag="ACZPD4_01235"
     CDS             2282..3274
                     /locus_tag="ACZPD4_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377736.1"
                     /translation="MLNTLNGLFIAAVLAGTPLLLGALGEILTEKSGNLNLGVEGMMF
                     MGAITGLAGSYYYEQAVIGSGGNPNGAVSSVIALLTSFAAGSLGALIYSFLTITLRAN
                     QNVTGLTLTIFGTGFGNFFGEYIGQKAGGYVAVSEVTKKAFSGIDIPVLSDIPVLGPL
                     LFRYNWVVYFAIAAALFMTWFFNRTRVGLNLRAVGEDPATADAAGINTTVYKYMATVI
                     GGGICGIGGMYMSMVTTSGVWVHNCVSGYGWLAVALVIFATWSPARGMLVALVFGGLT
                     IMRMYINIPGLPAQIYDMFPYIATILVLVITSMRRSKEHAQPKSCGLNYFREER"
     gene            3731..4822
                     /gene="recA"
                     /locus_tag="ACZPD4_01240"
     CDS             3731..4822
                     /gene="recA"
                     /locus_tag="ACZPD4_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1377737.1"
                     /translation="MAGNDEKKKALDAAIAKLEKDFGKGAVMKLGESGAHVAVETVPT
                     GCLSLDIALGLGGVPKGRVIEVYGPESSGKTTVALHMIAEVQKRGGIAGFIDAEHALD
                     PVYAKNIGVDIDELYISQPDSGDQALEIAETMARSGAIDIIVVDSVAALVPRQEIEGD
                     MGDSHVGLQARLMSQALRKLTPVISKSNCIVIFINQLREKVGVMFGNPETTTGGRALK
                     FYASVRMDVRKTETLKQSGEMVGNHTRIKIVKNKIAPPFKEAEFDIMFGKGISKEGDI
                     LDLATKLDLVKKSGAWYAYEGDKIGQGRENAKTYLSAHPEIMEELEKKVREHYQLGTD
                     SSEKDEKEVKKEKEQKGKSDDLALKGDEK"
     gene            4822..5436
                     /locus_tag="ACZPD4_01245"
     CDS             4822..5436
                     /locus_tag="ACZPD4_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR1377738.1"
                     /translation="MIVTKIEPCTKTKFKIYIDETFKFVLYKGEVSRFGIRIGEEIPP
                     ETEEKIRTEVLLKRAKLRAMHLLEDMDRTESALREKLRQGLYPSDVTEAAIEYVKSFG
                     YLDDVRYAENFVRSRQNVKSKKEIGTALLQKGISSEIAKRAMEICYEEMGEEEAIRKI
                     LRKKKFDPNKAEEAQMQKIYGYLGRKGFRYDTVRQVIQNYNDDA"
     gene            5533..7077
                     /gene="rny"
                     /locus_tag="ACZPD4_01250"
     CDS             5533..7077
                     /gene="rny"
                     /locus_tag="ACZPD4_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1377739.1"
                     /translation="MIAIILAAALALIVGMVSRFLTISGLKKNAESKIGNAEAKAREI
                     IDDAVKTAETTKKEALLEVKEESIKTKNDLEKETKERRAELQRYEKRVLAKEEAVDKR
                     SDVLEHRESKLTAKEDQLRQRESKVEELNQKRVQELERISGLTSEQAKEYLLKTVEED
                     VKHDTAKMVRELETQAKEEADKKAKEYVVNAIQRCAADHVAETTISVVQLPSDEMKGR
                     IIGREGRNIRTLETLTGVELIIDDTPEAVVLSGFDPIRREVARIALERLIVDGRIHPA
                     RIEEMVEKAQKEVDTMIREEGEAAALEVGVPGIHPELIRLLGRMKFRTSYGQNALKHS
                     IEVAQLSGLLAGEIGIDVRMAKRAGLLHDIGKSIDHDVEGSHIQIGVDLCRKYKESST
                     VINAVESHHGDVEPQTLIACVVQAADTISAARPGARRETIETYTNRLKQLEEITNQFK
                     GVDKSFAIQAGREIRVMVVPEQVSDDDMVLMARDIAKQIEFELEYPGQIKVNVIRESR
                     ATDYAK"
     gene            7185..>7701
                     /locus_tag="ACZPD4_01255"
     CDS             7185..>7701
                     /locus_tag="ACZPD4_01255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1377740.1"
                     /translation="MSEQIKRVNRELKFKGTIIDFYQDTMEINGDHTVVWDFIKHKGA
                     AAVVPVTEDGKILMVRQYRNALERYTLEIPAGALDEEGEPGFKCAARELEEETGYKSE
                     ELEWLITLRTTVAFCNEKIEVYVAKNLIPSKQHLDEDEFIDLKAQYRALKPEIDTNIQ
                     AVLDAGQFIGGP"
     assembly_gap    7702..7711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7712..7797
                     /locus_tag="ACZPD4_01260"
     CDS             <7712..7797
                     /locus_tag="ACZPD4_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1377741.1"
                     /translation="KEKIFTGEIEDAKTVASLMAYAVKYEA"
     gene            7924..8463
                     /locus_tag="ACZPD4_01265"
     CDS             7924..8463
                     /locus_tag="ACZPD4_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR1377742.1"
                     /translation="MKVFHVKKQLAALFMPGFLIGIIYVNFLAKKYVAAPDLFSEYFL
                     DQFRGVQIDEKEYLWYLLRIRCVPFFMLSGLALTKLKKVSAGLFLGWTGISVGVLVSL
                     AASNLGMKGCLLCLIGVSPQFLFYIPAYFMILKYCYESEDGRWNRQKTIFVGLMMSLG
                     IITELYVNPVLVKLFLNYV"
     gene            complement(8495..9892)
                     /locus_tag="ACZPD4_01270"
     CDS             complement(8495..9892)
                     /locus_tag="ACZPD4_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1377743.1"
                     /translation="MLFRSDKFRSFSSRNLFRGAAVLTAAGLITRIMGFFFRIFLSHA
                     FGEENVGLYQLIFPVYALCLSVSTFGIQTAVSHMVAEKMSSVVTYSAASSSSPHIERQ
                     KEAGNILAVSLGFTLFLSLIELYFLQKNAFYIATTFLGDARCENLLLIVSYALPFAAI
                     HSCICGYSLGLQQTKIPALSQLIEQSARILFVVLLFLFVKKTGEIPSVKLAAAGIAAG
                     ETAAALFSIQMLNRQKQLCYAASISSISKTFRKLSALSVPLTANRTAVTLLQSIEAAS
                     IPTFLKIYGMTSSEALRLYGVLTGMALPCILFPSALTTSIGTVLMPAVSAAQASDDNR
                     RIIRLLKQSVGSCFILGLGCCLFFLIFGNFLGRVLFHSSAAGSFILTLSWICPFLYTN
                     TALTSIINGLGKTFCTLLVNTAGLLVRIAGVFFAIPKFGIYGYLIGLLTSQFLVFGIA
                     VIILFFHLRKGAKDL"
     gene            10079..10507
                     /locus_tag="ACZPD4_01275"
     CDS             10079..10507
                     /locus_tag="ACZPD4_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1377744.1"
                     /translation="MKLSTKGRYGLRALIDLAQNGGEQPVSITSISTRQDISERYLEQ
                     LMSMLKKAGIVRSVRGAGGGYVLVKRLEDISVGDVLRALEGSLEPVDCAGLEPNGGCS
                     VSDSCVTKYVWKKINDSINQTVDEIKLDQLVKENGKNCRN"
     gene            10544..11731
                     /gene="nifS"
                     /locus_tag="ACZPD4_01280"
     CDS             10544..11731
                     /gene="nifS"
                     /locus_tag="ACZPD4_01280"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR1377745.1"
                     /translation="MDKLIYLDNAATTKTSPEVVEAMLPYFTEHYGNPSSIYSFAAAN
                     KEVISKQRETIAGVIGAKENEIYFTAGGTEADNWALTATAEAYGAKGKHIITTKIEHH
                     AVLHTCEYLQKRGYEVTYLDVDEYGVVDPKSVEAAIRPDTILISVMFANNEIGTVEPI
                     KEIGAIAHEKGILFHTDAVQAFCQIPIDVDECHIDMLSASGHKLNGPKGIGFLYIRKG
                     VKIRSLIHGGAQERRRRAGTENVPGIVGFGKAVEIAAASMERRMEKESQLRDYLIEKI
                     EKEIPYCRLNGDRERRLPNNVNFSFQYIEGESLLIMLDMKGICASSGSACTSGSLDPS
                     HVLLAIGLPHEIAHGSLRLTLGDDTTKEDLDYVTTCLKEIVARLRSMSPLYEDFLKKQ
                     KNK"
     gene            11816..12253
                     /gene="nifU"
                     /locus_tag="ACZPD4_01285"
     CDS             11816..12253
                     /gene="nifU"
                     /locus_tag="ACZPD4_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HIR1377746.1"
                     /translation="MYTEKVMDHFQHPRNVGELENASGVGTVGNAKCGDIMRIYLDID
                     ENQIIRDVKFKTFGCGAAVATSSMATELVKGKNIQEAMKVTNKAVMEALDGLPPVKVH
                     CSLLAEEAIHAALWDYAEKNGIKIEGLEKPKTDIHEDEEEEEY"
     gene            12253..13323
                     /gene="mnmA"
                     /locus_tag="ACZPD4_01290"
     CDS             12253..13323
                     /gene="mnmA"
                     /locus_tag="ACZPD4_01290"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1377747.1"
                     /translation="MKKVVVGMSGGVDSSVAAYLLKEQGYDVIGVTMQIWQEEDVCTV
                     EENGGCCGLSAVEDARRVAAALGIPYYVMNFREEFQKNVIDYFADSYVNGQTPNPCIA
                     CNRYVKWEALLKRSISIGADFIATGHYARVEQLSNGRYALRRSATAAKDQTYALYNLT
                     QEQLKRTLMPVGEYTKEEVRVIAEKIGLLVADKPDSQDICFVQDGNYAAFIEEHTGKK
                     ASEGNFVTSDGTVIGRHKGIIHYTVGQRKGLGLALGYPAFVLEIRPETNEVVIGTYEE
                     SLTTVVRAKQLNFMSVEDLKEPLRVFAKIRYNHKGAWCTVERTKEDEVTCCFEEPQRA
                     VTPGQAIVFYDGEYVLGGGTIL"
     gene            13337..>13863
                     /locus_tag="ACZPD4_01295"
     CDS             13337..>13863
                     /locus_tag="ACZPD4_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1377748.1"
                     /translation="MPKRLKETMIRSDFHMHTAFSTDSEEAVESMLNRAVERGLYSVC
                     ITDHMDADYPPDDEMGESAFRLDTENYFPFLLKKKKEYEERIQVRIGIELGLQKHLGT
                     RYETLIEKYPFDFVIGSMHLVCGEDPYTGKVFEELGDAQVYRRMFCETLECIRKIKCF
                     DVLGHLDYVVRYGKH"
     assembly_gap    13864..13873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13874..14185
                     /locus_tag="ACZPD4_01300"
     CDS             <13874..14185
                     /locus_tag="ACZPD4_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1377749.1"
                     /translation="EAYSYEKFSDEIDMILRELISSGKGLELNMAGIKYGLGFAHPHP
                     SVLRRYKELGGEIITVGSDAHKAEHIGYEFEIAGELLKSCGFRYYTEFENRKAVFRRI
                     P"
     gene            14331..15350
                     /gene="gap"
                     /locus_tag="ACZPD4_01305"
     CDS             14331..15350
                     /gene="gap"
                     /locus_tag="ACZPD4_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1377750.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFGAEGFEIVAINDLTSPKMLAHL
                     LKYDSTQGKYALADKVEAGEDSIIVDGKEIKIYAKANAAELPWGEIGVDVVLECTGFY
                     TSKAKAQAHIDAGAKKVVISAPAGNDLPTIVYNVNHEQLTKDDNIISAASCTTNCLAP
                     MAKALNDLAPIKSGIMCTIHAYTGDQMTLDGPQRKGDLRRSRAAACNIVPNSTGAAKA
                     IGLVIPELNGKLIGSAQRVPTPTGSTTILTAVVEGEVTVDQINAAMKAAANESYGYNT
                     DEIVSSDIVGMSYGSLFDATQTMALPTGDGTTEVQVVSWYDNENSYTSQMVRTIKYFG
                     KLLEA"
     gene            15500..>16631
                     /locus_tag="ACZPD4_01310"
     CDS             15500..>16631
                     /locus_tag="ACZPD4_01310"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1377751.1"
                     /translation="MAALNKKSVDDINVKGQRVLVRCDFNVPLIDGKITDENRIVAAL
                     PTIKKLIADGGKVILCSHLGKPKGEPKPELSLAPVAVRLAELLGQDVKFAADPEVVGE
                     NAKAAVEAMKDGDVVLLENTRYRAEETKNGEAFSKELASLCDVFVNDAFGTAHRAHCS
                     NVGVTEYVDTAVVGYLMQKEIDFLGNAVENPERPFVAILGGAKVSSKISVINNLLDKV
                     DTLIIGGGMSYTFTKAQGGSVGASLLEEDYLQYALDMIKKAEEKGVKLLLPVDTIVAD
                     EFSNDANTKVVSRDAIPDGWMGLDIGPETAKIFAEAVKSAKTVVWNGPMGCFEMPKFA
                     EGTKVVAEALANTEAVTIIGGGDSAAAVNQLGFGDKMTHISTG"
BASE COUNT         5188 a         2757 c         4426 g         4240 t
ORIGIN      
        1 agcagaatta gcacaaaaat atggctttga tcttgatccg acaaagaaag tttacaatat
       61 gtcggtttct gaaaaacaaa cggtggaaat attaaaagtg ctgtacaaag gcgcggatat
      121 tttgatttta gacgaaccta cggcggttct tactcctcag gaaacggaaa agctgtttgc
      181 tgttttgaaa aacatgaaaa aacacgggaa atcgatcatc atcattacac ataaattaaa
      241 tgaagtaatg gcgatatcag atcgtgttgc gatcttgcga aagggagaat atatcggagt
      301 tgtggaaact tcggaaaccg atccggatca acttacggaa atgatggtcg gccggccgat
      361 cagtcttgag attgaaagac cggaagtaaa acggggcgga aaacgtcttc gggtgatcga
      421 tcttacctgt ttgagcggag acggagtgaa ggctcttgat aatgtttcgt ttgatgctta
      481 tgcaggagag atactgggaa tcgccggaat tgcaggaagc ggacaaaaag aactgtgcga
      541 gacgattgcg ggactttatc cttctgtcgg gggatctgtt ttgtattctg aatcatcaga
      601 aaacggggcg gtaaaagaac gtattttagg attaagcccg gatgaaatcg ccaaaaaagg
      661 aatttcaatg gcatttgttc cggaagatcg tcttggaatg ggtcttgttg ccggaatgga
      721 tatgacagat aatgtcatgc ttagaagtta taagtcgaac aaaggtattt ttgtagatcg
      781 gaaaagtccg agagatcttg cggaaaaact gattaaggaa ttagaaatcg taactccggg
      841 cgtggaaact ccggtaggaa gactttcggg aggtaatgtg cagaaagttc ttttaggaag
      901 agagatagcc tgtcagccgt cggttctgat tgtcgcatat ccggtaagag gtcttgatat
      961 caatgcgtct tacagaatct atgatctttt gaacgaacag aagaaaaagg gagttgcggt
     1021 tgtatttatc ggagaagatc ttgatgtgct tatgcagctt tcggaccgca tcatggttat
     1081 gtgcggagga aaggtaagcg gtattgtcga tccgagaaaa gtgacaaaag aagaaatcgg
     1141 attaatgatg atccgtacag aacaagatgg agaaaaagag gaggtgacag aatgagttcc
     1201 aatgtgacac atgcgaaaga gcctttgttc cgaatggtaa aaagagatgc gattcctcag
     1261 aaaaaagcgt ggatgatccg tgcggctgct tttcttttat ctttggctac gggcgctgtc
     1321 gtgatcatga tgctcggaca taatccgttt tcggtatata tttccatgat aaagggagca
     1381 tggggaacga agactgtttc ttatgagacg attaagatcg caattccgct tcttatcacg
     1441 gcgatcggaa tttcttttgc gttcaaaatg aaattctgga atataggggc ggaaggacag
     1501 attttggtag gtgcagttgc ggcaggtgcg tttggtcttt ttactgctca tatttttccg
     1561 aagatcccac ttgtaattct tatgatgctg gcggcgatcg tagcgggcgg tttgtacggt
     1621 ttgctcccgg ctgtctgcaa ggcgaagtgg ggaacaaatg agacgttgtt tacactgatg
     1681 ttaaattata ttgctctggc atttatcaaa tatcttatga acggcccgtg gaaagctccg
     1741 ggaagcagct atccgaaaat agcgatgctt gttccgggag cgcgtttgac aaaagtattc
     1801 ggagtgcact ggggatggat cttagcgctt attctcgttg tcgtatctta tatttatttt
     1861 aataagacga aacaaggcta tgaaattgct gttgtaggtg aaagcaataa tactgccaga
     1921 tatgcgggaa tcaatgtggg acgggtattc cgcagaacga tgtttttatc cggagcgctt
     1981 tgcggacttg tcggaatgct tcagtttgcg ggatcggact ttacgattac ggaaggaact
     2041 gcaggcggcg tcggatttac ggccattaca gttgcatggc tcgcaaaaat gaatccggcg
     2101 ggaatgttgg ttgtatcagt atttatcgcg atgcttgagc gaggggcgaa cgcaattcag
     2161 acacagttta aaattccggc atctgcggcc gatctgttga ttggtattat tttgttcttt
     2221 atgctcggat gtgagttttt tatcacatat aagttgatcg gaagaggaaa ggaggaacat
     2281 catgcttaat acgttgaatg gattatttat tgcggcagta ttggccggaa cgccgcttct
     2341 tttaggagcg ctcggtgaaa tactgacaga aaaatcagga aacttaaatc ttggtgtaga
     2401 gggaatgatg ttcatggggg cgatcacggg actggcaggt tcgtattatt atgaacaggc
     2461 agtgatcggt tcgggcggga acccgaacgg agcggtttct tctgtcatag ctcttcttac
     2521 ttcttttgcg gcgggttctc ttggggcact gatctacagt tttttgacga ttacgctccg
     2581 ggcaaatcaa aatgtaacag gtctgacatt aacaattttc ggaaccggtt tcggaaattt
     2641 tttcggagag tatatcggac agaaggccgg cggttatgtg gcggtatctg aagtgacgaa
     2701 gaaagcattt tcagggattg atatcccggt tttgtcagat atcccggtgc ttggtcctct
     2761 tttattccgg tataactggg tcgtttattt tgcaatcgcg gcagcgcttt ttatgacatg
     2821 gttttttaat cgtacaagag tgggactgaa tcttcgtgct gtcggggaag atccggcaac
     2881 tgcggatgcg gcgggtatca atacgacggt atataaatat atggcgactg tgatcggcgg
     2941 agggatctgc ggaatcggtg gtatgtatat gagcatggtt acaacatcag gcgtatgggt
     3001 gcataactgt gtcagcgggt atggatggct tgcggtagcg cttgttattt ttgcaacatg
     3061 gagtcctgca agaggaatgc ttgtggcgct tgtattcggc ggtcttacga tcatgcgtat
     3121 gtatattaat attcccggac ttccggctca gatttatgat atgttcccgt acattgctac
     3181 gatacttgtt cttgtgatca caagtatgcg cagaagtaag gagcatgcac agccaaagag
     3241 ctgcggactg aattatttcc gcgaggaaag ataaagggat tcgtatatga atattttatt
     3301 cagctatgca tagtgatttc cgatgacggt aatgggtttt gatatgtttt cggaaaagtg
     3361 catagctgaa tttttgtata agcgacgggt caaagtcggg gtgtgaccga gatcttggcg
     3421 aatgtttata ataccggaat gtgcttttgg aacttccggt aattgtttta tgtacggttg
     3481 ttctggctgt ggaagctgcg caatgataac tgtcatggga ttgtgggagg catagaaata
     3541 tgatatccta aatgaaaatt tattgtatga aggctgatcg gcattttggg gttataccgt
     3601 gctgaaaaat attcggatat attattcgca aaaatagtgt tgacaattta aagagggtat
     3661 aatactataa tagcagaagc gaacaactgt tcgatactaa aaatcgttat acagaaggag
     3721 atattttatt atggcgggaa atgatgaaaa gaaaaaagcg ctggatgcgg cgatagcaaa
     3781 acttgagaag gattttggaa agggcgcggt gatgaaactc ggagaatcag gagctcatgt
     3841 tgcggtagag acagttccta caggctgttt gagtcttgat attgcgcttg gattgggcgg
     3901 agttccgaaa ggacgcgtga ttgaagttta cggacctgag tccagcggta agacaacggt
     3961 tgcgcttcat atgattgcgg aagtgcagaa acgcggcggt attgcgggat ttattgatgc
     4021 ggaacatgcg ttggatccgg tctatgcaaa gaatatagga gtggatattg acgaactata
     4081 tatttcccaa ccggacagcg gtgatcaggc tttggagatt gcagagacga tggcacgttc
     4141 cggcgcaatc gacattatcg ttgtagactc tgttgcggct cttgtgccga gacaggagat
     4201 tgaaggagat atgggagaca gtcatgtggg acttcaagcg cgattgatgt cacaggcact
     4261 taggaaactg actccggtta tcagtaagtc gaactgtatt gtgattttta tcaatcagct
     4321 gagagaaaaa gtcggggtca tgttcggaaa tccggagacg acaaccggag gaagggcgct
     4381 taagttttat gcgtctgtcc gaatggatgt cagaaaaaca gagactctga aacaaagcgg
     4441 agagatggtc ggaaaccata cccgtattaa gattgtgaaa aataagatag ctccgccgtt
     4501 taaggaggcg gaatttgaca ttatgttcgg aaaaggaatt tcaaaagaag gagatatcct
     4561 tgatctggct actaagttag atcttgttaa aaagagtgga gcatggtatg catatgaagg
     4621 cgataagatc ggacagggcc gggaaaatgc aaagacatat ctgtctgctc atccggaaat
     4681 aatggaagag ttggaaaaga aagtaagaga acattatcaa cttgggacag attcttcgga
     4741 aaaagatgag aaagaagtga aaaaggaaaa agaacaaaaa gggaaaagtg acgatcttgc
     4801 attaaaaggt gatgaaaaat aatgatagtc actaaaatag agccatgtac aaaaacgaag
     4861 tttaaaattt atatagatga gacatttaag tttgtacttt ataaaggaga ggtttcccgt
     4921 ttcggaattc ggatcgggga agaaatcccg ccggaaacag aagaaaagat ccgaacggaa
     4981 gtgcttttga agagggcgaa gcttagggcg atgcatcttc tcgaggatat ggaccgaaca
     5041 gagtctgccc ttcgcgaaaa acttcgtcag ggtctgtatc cgtctgacgt gacagaggcg
     5101 gcgattgaat atgtaaagtc attcggttat cttgatgatg tccggtatgc ggaaaacttc
     5161 gtgaggagca ggcagaatgt gaagagtaaa aaagaaatcg gcacggcgct gctgcagaaa
     5221 gggatttcgt cggaaatagc aaagcgcgct atggaaatct gctatgaaga aatgggagaa
     5281 gaagaggcaa tccgcaaaat tttaagaaaa aaaaagtttg atccgaataa ggcggaagaa
     5341 gctcaaatgc agaaaatata cggctatctg gggcgcaaag gatttcgtta tgatacagtc
     5401 cgtcaagtga tacaaaatta taatgacgat gcttgacatt gttgtgaaaa cagtataaaa
     5461 tttaactgtt gtatttaagt aatatgtaca atgaaaattt aataaggagg tgctcctgtg
     5521 caagtagatt tagtgatcgc tattatactt gctgcggcac tcgctttgat agttgggatg
     5581 gtttctcgat ttctcacaat ttccggtttg aaaaaaaatg cagaaagtaa aatcggaaat
     5641 gcagaggcaa aagcaagaga aatcatcgat gatgctgtga aaactgctga aacaacgaaa
     5701 aaggaagctc ttctcgaagt gaaagaagaa tcgatcaaga caaagaatga tcttgagaag
     5761 gagacaaaag aaagaagagc ggaattgcag cgctatgaaa aaagagtgct ggcaaaagaa
     5821 gaggcagttg ataagcgttc tgatgtgtta gaacatcggg agtcgaagct tacggcgaaa
     5881 gaagaccagc ttcgtcagcg ggaatccaag gtagaagagc tgaatcagaa acgtgtgcag
     5941 gaactagaac gaatctcagg acttacctcc gaacaggcaa aagaatatct gttgaaaact
     6001 gttgaagaag atgttaagca tgacaccgca aaaatggtca gagaattgga aacacaggcg
     6061 aaagaagaag cagacaagaa agcaaaagaa tatgttgtca atgcaattca gagatgtgcg
     6121 gcagaccatg ttgcggaaac gacaatttcc gttgtgcagc ttccgagcga tgaaatgaaa
     6181 ggtcgtatta tcggtcgtga aggaagaaat atccgaacac ttgaaacatt gaccggtgtg
     6241 gaactgatta ttgatgatac accggaagcg gttgttttat cgggatttga tcccatccgc
     6301 cgtgaagttg caagaattgc acttgaacgt ttgatcgtag acggacgtat tcatccggcg
     6361 agaattgaag aaatggtaga aaaagcgcag aaagaagtcg atacaatgat cagagaagaa
     6421 ggagaagcgg cagcgttaga agtaggcgtg ccgggaattc atccggagtt gatcagactt
     6481 ctcggtcgta tgaagtttag aacaagctat ggacagaatg cgttgaagca ttcgattgaa
     6541 gtggctcagt tgtcgggact tcttgcgggc gaaatcggta tagatgtcag aatggcgaaa
     6601 cgtgccggac ttctgcatga tataggaaaa tctatcgacc atgatgtaga agggtcacat
     6661 attcaaatcg gtgtggatct ttgtaggaaa tataaagaat cctcaaccgt aattaacgcg
     6721 gtggaatcgc atcacggaga tgttgagccg cagacattga tcgcatgtgt agtgcaggca
     6781 gccgatacga tttcggcagc aagaccgggt gcaagacgcg agacgatcga aacatacacc
     6841 aacagattga aacagttaga agagattacc aaccagttta aaggggtgga taaatctttt
     6901 gccattcagg cgggcagaga gattcgagtt atggtagttc cagagcaggt atctgatgat
     6961 gatatggtgc ttatggcaag ggatattgca aaacaaattg aatttgaact tgagtatccc
     7021 ggacagatta aagtcaatgt aatccgtgaa tcacgggcaa cagattatgc gaaatagcaa
     7081 tgaatcataa cagcagacga aagaccagcc cttgtcgaaa gacgagggct ttcttttatt
     7141 aaaatatgtt gattgctgag aggatacggg gaaggagaaa gacgatgagt gaacagatta
     7201 aacgtgtgaa cagagaattg aaatttaaag gaacgattat tgatttctat caagatacaa
     7261 tggaaattaa tggggatcat acagttgtat gggattttat caagcacaaa ggagctgcgg
     7321 cagtagttcc ggtcactgaa gatggaaaga tacttatggt acgtcagtat cgaaatgcac
     7381 ttgagagata tactttggag ataccggcag gggctctgga tgaggaagga gagccggggt
     7441 ttaaatgtgc tgctcgggaa ttggaagaag aaacaggtta taaaagtgag gaattggaat
     7501 ggctgatcac cttgaggaca acggttgctt tttgcaatga gaagatcgag gtgtatgtgg
     7561 cgaaaaattt aatcccttca aaacagcatt tagatgagga tgaatttatt gatctgaaag
     7621 cacaatatcg tgcactgaaa ccggagatcg acaccaacat tcaggcggtc ttggatgccg
     7681 ggcagttcat cggcggtccc tnnnnnnnnn ntaaaagaaa aaatatttac aggagagatt
     7741 gaagatgcaa aaacagttgc atcattaatg gcatatgcag taaaatacga agcctgaaaa
     7801 tgatgtaaga aacggatcgt gaaaagtgat aatgaaaaaa ttccggcata taatgaaata
     7861 tcaataaaaa gtaaagtgca gaatggcaaa aggatcagca actgcgcgat acagggagga
     7921 ataatgaagg tatttcatgt gaaaaagcag ttagcggctt tgtttatgcc gggattttta
     7981 attggaatta tttatgtcaa ttttcttgct aaaaaatatg tggcagctcc ggatctgttc
     8041 agtgaatatt ttttggatca attcaggggt gtgcagatag acgaaaaaga atatttatgg
     8101 tatctgcttc gcattcgctg cgttccgttt tttatgcttt cagggttggc tcttacgaag
     8161 ttgaaaaaag taagcgccgg gttatttctt gggtggacag gcatatctgt gggggtgctt
     8221 gtttcgctgg cggcatcgaa cttgggaatg aaaggctgtc tcttgtgtct tataggtgtt
     8281 tctccacagt ttcttttcta tattcctgcg tattttatga tcctgaaata ttgttatgag
     8341 tcagaagatg gcagatggaa cagacagaaa acaatttttg tcggtttgat gatgagttta
     8401 ggaattatta ccgaattata tgtgaatcct gtattagtga agttgttctt gaattatgtg
     8461 tagttgtcta tagactatgt gctgcgcaca tagtctatag atcttttgct ccttttcgga
     8521 gatgaaaaaa taaaataata acggcgattc cgaaaacaag aaactgactg gttaaaagtc
     8581 cgatcagata accgtatatt ccaaacttgg ggattgcaaa aaatactccg gcaatacgaa
     8641 caagaagacc ggcagtgttg acgagaagtg tgcagaatgt tttcccaagt ccgttaatta
     8701 tgcttgtaag ggcggtattt gtgtaaagaa acgggcagat ccatgaaagt gttaaaatga
     8761 aacttccggc agcagaacta tggaacaaaa ctcttccgag aaagtttccg aaaatcagaa
     8821 aaaacagaca gcatccaagg cctaaaatga aacagcttcc tacagattgt ttcaataaac
     8881 gaatgattcg tctgttatca tcagatgcct gggcagcgct gacggcaggc ataagcactg
     8941 ttccgataga agttgtgagc gccgaaggaa aaaggatgca tggcagagcc attcctgtca
     9001 atactccgta aaggcgaagc gcttccgaag aagtcattcc gtagattttt aaaaaagtcg
     9061 ggatggaggc cgcctcaatg ctttggagta aagtaactgc cgttcgattt gctgtaagag
     9121 gaacagataa agcggacagt tttctgaaag tttttgaaat ggaagaaatc gaagcggcat
     9181 aacaaagctg cttttgccga ttcagcatct gtatcgaaaa aagtgcggca gccgtctcgc
     9241 ctgcggcaat gccggcggcg gcaagtttga cagaagggat ttccccggtt tttttaacaa
     9301 agagaaaaag caggacgacg aaaagaattc gagcggattg ttcgataagc tgtgacaggg
     9361 caggaatttt tgtctgctga agccctaaac tgtatccgca gatacaacta tgtattgcgg
     9421 cgaacggtaa tgcataagag acgataagaa ggagattttc acacctggca tctcctaaaa
     9481 aagttgttgc tatataaaag gcgttttttt ggagaaaata taattctata agtgagagaa
     9541 agagtgtgaa gccaagagaa actgcaagaa tgtttccggc ttctttttgc cgttcaatat
     9601 gcggggagga tgaactggcg gcagagtaag taacaacaga tgacattttt tctgcgacca
     9661 tgtgtgagac ggcggtttga atcccgaatg tgctgactga cagacaaagt gcatagaccg
     9721 ggaaaatgag ctgatataaa ccgacgtttt cttctccgaa agcatgactt aaaaaaatac
     9781 gaaagaagaa tcccataatg cgagtgatca atccggcggc agttaaaacg gcggcgccgc
     9841 gaaataaatt gcgggaagaa aaggagcgga atttgtcaga acggaataac ataaaatcac
     9901 ctcgatttgt aacaacagac atatacttgt taaaacatat gtcataagga gacttgacag
     9961 aaccggggat gaatgctatg ctacaaaaga aatccctagt aaaatactgg ggaatccatt
    10021 ctgcaatatt aagaataaag aaatagagta tttttattaa ggaacggagc gtgagaaagt
    10081 gaagttatct acaaaaggaa ggtatggact tcgtgcgttg atcgatctgg cgcaaaatgg
    10141 aggtgaacag ccggtatcta ttacaagtat ttcaacaaga caagatatct cggaacgata
    10201 tcttgagcag ttgatgtcta tgctgaaaaa agcggggatt gtaagaagtg taagaggagc
    10261 cggaggcgga tatgtgcttg taaaaagact ggaagatatt tctgtgggag atgtgcttcg
    10321 cgcattggaa ggcagtcttg agccggtgga ctgcgcgggt cttgaaccaa atggaggatg
    10381 cagtgtttcg gacagctgcg tgacaaaata tgtttggaag aaaattaacg acagtattaa
    10441 ccagacggta gatgagatta aacttgatca actcgtaaaa gaaaacggaa aaaactgtag
    10501 aaactgatta cagtttattt aatgaagaga tggaggaaaa aagatggata aactgatata
    10561 tcttgataat gcggctacga caaaaacttc acctgaagtt gtggaagcaa tgctgcctta
    10621 ttttacagaa cattatggga atccgtccag tatatattcg tttgcggcgg caaataagga
    10681 agtgatttca aaacaaagag agacgatcgc aggtgtgatc ggagcaaagg aaaatgagat
    10741 ttattttacg gcgggaggaa cggaagccga caactgggca ttgacggcga cagcagaggc
    10801 gtatggcgca aaaggaaagc atattattac gacgaagatt gaacatcatg ccgttttgca
    10861 tacatgtgaa tatcttcaaa agcgagggta tgaggttact tatctcgatg tggatgaata
    10921 tggagttgta gatccgaagt cggtagaggc ggcgatcaga ccggatacga tattgatctc
    10981 ggtcatgttt gcgaacaatg agatcggtac ggtagagccg ataaaagaga tcggcgcgat
    11041 cgctcatgaa aaaggaattt tgtttcacac ggacgccgta caggcgtttt gtcagatacc
    11101 gatcgatgtc gatgaatgtc atatagatat gctcagcgcc agcggacata aattaaacgg
    11161 tccgaaggga attggatttt tatacatcag aaaaggagtc aaaatccgct cgttgatcca
    11221 cggaggagca caggagagga gacgtcgtgc aggaactgaa aatgttccag gaatcgtcgg
    11281 tttcgggaaa gcagttgaaa tagctgcagc gtctatggaa agaagaatgg aaaaagaaag
    11341 tcagcttcgg gattatttga tcgaaaagat tgaaaaagag ataccatact gcagattaaa
    11401 cggcgacaga gaaaggcgtc ttccgaataa tgtgaatttc agttttcaat atatagaagg
    11461 cgagtcgctt cttatcatgc tcgacatgaa aggcatctgc gcttccagcg gatctgcgtg
    11521 tacatcggga tcgctggatc cttcacatgt tcttttggca atcgggcttc ctcatgaaat
    11581 tgcacacgga tctttgcgtc tgacgcttgg agacgatacg acaaaagaag atctggatta
    11641 tgtgacgacc tgtttgaaag agatagtagc tcgactaaga agcatgtctc ctctttatga
    11701 agattttttg aaaaaacaaa aaaataagta aaggaacagg gaaaagaaat tgacttaagt
    11761 caaaagaaaa atatcgtttg aataagcgga aacagagaat aggaggaatt tatttatgta
    11821 tacagaaaaa gtaatggatc attttcagca tccgagaaac gtaggagaac ttgaaaacgc
    11881 cagcggcgtc ggaacagtgg gaaatgcaaa atgtggagat atcatgagaa tttatcttga
    11941 cattgatgaa aaccagatta ttcgggatgt gaagtttaaa acttttggat gtggggcggc
    12001 tgtagcaaca agcagtatgg cgacagaact tgtaaaaggg aaaaatattc aggaggcaat
    12061 gaaagtaaca aacaaagctg tgatggaggc tcttgatggt cttccgccgg taaaagtaca
    12121 ttgttcgttg ctggcagagg aagcaattca tgcggcgctt tgggattatg ctgaaaagaa
    12181 cgggatcaag attgaagggc tggaaaaacc aaagacagat attcatgagg acgaggaaga
    12241 agaggaatat taatgaagaa agtggttgta ggaatgtcag gaggcgtgga ttcgtctgtg
    12301 gcggcatatc tgctgaaaga acaaggctat gatgtgatcg gcgttacgat gcagatctgg
    12361 caggaagaag acgtctgtac agtagaagaa aacggaggct gctgcgggtt aagcgctgtt
    12421 gaagatgcga ggagagttgc agcggcattg ggcattcctt attatgtgat gaatttcaga
    12481 gaggaattcc aaaaaaatgt aatcgactat tttgcagatt cttatgtgaa cggccagacg
    12541 ccgaatccgt gtattgcatg caaccgttat gtgaaatggg aagcgcttct taagcgcagc
    12601 atctcgatcg gagcagattt tattgcgaca gggcactatg caagagttga gcagctttca
    12661 aacgggagat atgctctgcg gcgttcggca actgctgcaa aagatcagac ttatgcatta
    12721 tataatctga cgcaggaaca gttgaaacga acgttgatgc cggtaggaga atatacaaaa
    12781 gaagaagttc gggtaattgc ggaaaagatc ggactgctcg ttgcggacaa gccggacagt
    12841 caggatatat gttttgtaca ggatggaaac tatgctgctt ttatagaaga acatacaggg
    12901 aagaaagctt cggaaggaaa ctttgttaca tcggatggaa ctgtcatagg gaggcataaa
    12961 gggattatcc attatacggt cggtcagaga aagggacttg gtctggcact tggatatccg
    13021 gcatttgttc ttgaaatccg tccggaaacg aatgaggtcg ttatcggaac ttatgaagaa
    13081 tctctgacaa cggttgtccg tgcaaaacag ctgaacttta tgtcggtaga agatctaaaa
    13141 gaaccgctgc gtgtgtttgc gaagatcagg tacaatcaca aaggcgcgtg gtgtacggtc
    13201 gagcggacga aagaagatga agtaacttgc tgttttgagg agccgcagcg cgcggtgaca
    13261 cccggtcagg cgattgtatt ttatgacggc gagtatgtac ttggcggcgg tacgattttg
    13321 tgagaggtgt gagaggatgc ctaaacgatt aaaagaaaca atgatacgat ctgattttca
    13381 tatgcatacc gcattttcaa cggacagtga agaggctgtt gaaagtatgt tgaaccgggc
    13441 tgttgaaaga gggctctatt cggtttgcat cacagatcat atggatgcgg attacccgcc
    13501 ggatgacgaa atgggagaaa gtgcattccg gttggatacg gaaaactact ttccgtttct
    13561 tttaaaaaag aaaaaagagt atgaagaacg gattcaggtg cgtatcggaa ttgaattagg
    13621 acttcaaaag catctgggga caagatatga gacgttgatc gagaagtatc cgttcgattt
    13681 tgtgatcggc tctatgcatc ttgtctgtgg ggaagaccct tataccggaa aggtctttga
    13741 agagctggga gatgcacagg tatacaggag gatgttctgt gaaacgttgg aatgcattcg
    13801 gaaaataaaa tgtttcgatg tgctcggtca tttggattat gtggttcgat atggaaagca
    13861 tcannnnnnn nnngaggcgt attcttatga aaagttttct gatgagattg acatgatatt
    13921 aagagaattg atctcgtcag gaaaaggtct ggaattgaac atggcaggaa taaagtatgg
    13981 tcttggattt gctcatccgc atccgagtgt gcttcgccga tataaagagc tgggaggaga
    14041 gatcatcacc gtgggatctg atgctcataa agcagagcat atcggatatg aatttgagat
    14101 cgcgggcgaa cttctgaaat cctgtggttt tcgatattat acagaatttg agaatcgtaa
    14161 ggctgttttt cgacggattc cataaaaatg tatttaggac ttgaattttt gttcctgttt
    14221 tagtacaatt atgctagttg atgaattctt aacaatgttt actttgcggg ggttcatttg
    14281 cataactgga aacagttaaa tatttttcaa acttttagga ggaattaatc atggcagtaa
    14341 aagtagcgat taatggattt ggacgtattg gacgtcttgc tttcagacag atgttcggag
    14401 cagagggatt tgagatcgta gcaatcaatg acctgacatc cccgaaaatg cttgctcact
    14461 tactgaaata tgactcaaca cagggcaaat atgcactggc agataaagtt gaggctggag
    14521 aggattctat catcgttgat ggaaaagaaa ttaaaatcta cgcaaaagca aacgcagcag
    14581 aacttccgtg gggagaaatc ggcgttgacg tagttctgga gtgtacagga ttctacacat
    14641 ctaaagcaaa agcacaggct cacatcgatg caggcgctaa aaaagttgtt atttccgctc
    14701 cggcaggaaa tgaccttccg acaatcgtat ataacgtaaa ccacgagcag cttacaaaag
    14761 atgacaacat catctcagca gcttcctgta caacaaactg cttggctccg atggcaaaag
    14821 cactgaacga tcttgcaccg atcaaatccg gtatcatgtg cacaatccac gcttacacag
    14881 gtgatcagat gacacttgac ggaccgcaga gaaaaggcga tctgagaaga tctcgtgcag
    14941 cagcttgcaa catcgtgccg aacagcacag gtgcagcaaa agcaatcggt cttgttattc
    15001 cggaactgaa cggaaaactg atcggttctg ctcagcgtgt tccgactccg acaggatcta
    15061 caacaattct tacagcagtt gtagaaggcg aagtaacagt tgatcagatc aacgcagcta
    15121 tgaaagcagc agcaaacgag tcttacggat acaatacaga tgagatcgta tctagcgata
    15181 tcgtaggtat gtcttacgga tcactctttg atgctactca gacaatggca cttccgactg
    15241 gcgacggaac aacagaagtt caggttgttt catggtatga caacgagaac tcttacacaa
    15301 gccagatggt aagaacaatc aaatacttcg gaaaactgct tgaggcttaa ttcattttaa
    15361 agtaatggat tattttggcg gacaggcagt gtcgcgccga acagttatga aaatgactca
    15421 caacggggtc cggtcttatt gtaggaccgg actccgtttt gttatgaatc ttcagaaaaa
    15481 tattcaggag gcaatgataa tggcagctct taataaaaaa tcagtagatg atattaatgt
    15541 aaagggacag agagtccttg taagatgtga ctttaacgtt ccgctgatcg acggtaagat
    15601 tacagatgaa aatcgtatcg tggcagctct tcctacaatt aaaaaattga ttgcggatgg
    15661 aggaaaagta atcctttgtt ctcacctcgg aaagccgaag ggtgagccga aaccggaact
    15721 ttctcttgca ccggtggcag taagactggc tgaacttctc ggacaggatg tgaaattcgc
    15781 agccgatccg gaagtagtag gagaaaatgc aaaagcggca gtggaagcga tgaaggacgg
    15841 cgacgtagtc cttcttgaga atacacgtta cagagcagaa gagacaaaga acggagaggc
    15901 attcagcaaa gaactggctt ctctttgtga tgtatttgta aatgatgcgt ttggtacggc
    15961 tcacagagca cactgttcaa acgtaggagt tacagagtat gttgatacgg cagttgtcgg
    16021 atatcttatg caaaaagaga tcgacttcct tggaaatgca gtagagaacc cggaaagacc
    16081 gtttgttgcg atccttggcg gtgcgaaagt ttccagcaaa atctcagtta tcaataacct
    16141 gcttgacaaa gtagacacat tgattatcgg aggaggaatg agttatacat ttacaaaggc
    16201 tcagggcgga agtgtaggag catctcttct tgaggaagac tatcttcagt atgcacttga
    16261 tatgatcaag aaagctgagg aaaaaggagt aaaacttctt cttccggttg acacgatcgt
    16321 agcagatgaa ttttcaaacg atgcaaatac gaaagttgtt tcacgtgatg cgattccgga
    16381 cggatggatg ggtctggata tcggaccgga gacggcaaaa atctttgcag aggcagtgaa
    16441 atctgcgaaa acagttgttt ggaacggacc gatgggatgc ttcgagatgc ctaaatttgc
    16501 agaaggaaca aaagttgttg cagaagctct cgcaaataca gaggctgtta cgatcatcgg
    16561 aggcggtgat tcggcagcgg ctgtaaatca gcttggattc ggcgataaga tgacacatat
    16621 ttctacaggc g
//
LOCUS       DBMDXF010000015        16615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1044_length_16615_cov_2.612632, whole genome shotgun sequence.
ACCESSION   DBMDXF010000015 DBMDXF010000000
VERSION     DBMDXF010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_16615_cov_2.612632"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            25..10404
                     /locus_tag="ACZPD4_01315"
     CDS             25..10404
                     /locus_tag="ACZPD4_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy0128 family protein"
                     /protein_id="HIR1377752.1"
                     /translation="MGNTTASFQPAKKNTFYYFTEDTPIYTDKDCTMAVKNEPSADQT
                     YYYKRPYVQLNGGQVEEIDGKVAFTGSNFQQASANFGINDKGEYFIKSGSARLTRIDA
                     LIDDKTENITNTADVVIDPIWDNINNPDYLNVYLGNNGRLALELPGALAISKDARVAA
                     NKNLNADEILKDKKFTFEISIPSMKGKTVKAEVRNAQNEIQGKAFELEFDKTGAAKYQ
                     IKDDETLYIHGLDKDAQYTVTEKELPKGFELTAVDKKADAKEAAGTIVSGKSVKHVFE
                     NTYDVDEITLAETDFASYEKQFDRWDIADKFRIELIPTTEGAPLPNGAADGKKGVEVT
                     EKNSSGNFGEITFSHPGVYEYEIQEKQPASAIPGVIYSLASYTYRVTVTDNGDGTLSA
                     VAEMEKTANDDGASVGNTPIPVENKTAVFVNDFHADSATTSILAKKVYADESGANPLK
                     NGMFEFKLKATGDNAEQAPMPTGEKDENGYIHVVNVGTGITFGNMVFTEENVSDTPWT
                     YEIAEVIPETAVNNGDGTYTLNGITYKAQTYSVSIMAKAQGSGEDAYIVIEKTYSKAE
                     GAVAEDQVVFNNSYEPKPIVLPGEGESAVQGRKTLVGRDSLVDEEFSFTLSAANTAAR
                     TGLKENFIIFNGDTAQDTMQKTVNGLTNNQAATFDFDSIEFKRPGTYVFSVVENAPEN
                     GNGMVYDRHTARVRVIVTDENGELKAETAYYNGEGADTDAAAFVNLYTASATYGTGAE
                     LIVEKTLSGRALKAGEFTFKIEAADSDTVNAETADAKLSDSDREFTNTSGAADGVASR
                     IFDKLSGVTFTQDDAGKTFSYVLSEAAGNLPGVTYSKDTYRFDITVVDNGDGTMHTET
                     KVTMQGDGVTAEAHHDSSDGRDTIVAGFTNGYHADSVEVDFTEAAALFHKRLVGRDWK
                     EDDSFTFNLTAEDGTPMPKDTEGNDVTSVTVSQKGGTEDGTDVPFGFGKVTYDTVGKY
                     SYTVTEENAGQTINGVTYSKNEAKIIVDVSDPGNGQLTANIQSYSTIFINEYGASLNL
                     GAAGGLAITKTVTGHALAKEQFEFKVKAVKTDTATADEAAELFGFKEGETEVTFKNNE
                     AAADGQTVTMLKTDTNFTLKNLGKVYKYEVSEMNDKKPGYTYDDTVYTVELWVTDDGD
                     GTLTLHTKVTDEKGSVISEETSNETDQKQTVLAFNNSYAGSGVLDGSANLAGTKKMDG
                     PWEATDKDLSGFRFTITGGDEATNAAITAGTVVLPQSVTATSDADGNFNFGDITFNEK
                     GTYKFTVSEVVPAEGDKIPGVAYNAETVTIIVNVTDNDDGTLTAALAEGSQELIFTNT
                     YATTQDATFTPSVVKEVKGLNAKEIFTFKLSAADEATKAAIDSGTLTGIGTTADLYSS
                     EKTTTKLIPKDGTEQVDFNALTFKKAGTYKFTVQETNANAPTGWTYDSHTYEIIIKVT
                     DQDSVLKATQEINADGVTNSQIFINKFEASTTYGDEGGLNVTKTLNGRTLAADMFDFT
                     ITGEATDSVTAEEAEAKLAETDKSFKNTAPGKDDVAVMSKLSDVKFDETDIGKTYQYS
                     VREAAGTDTKYTYDQVSATVAITVQEKDGELYTVTTVTKGDDSKEYSSAESKATAVAP
                     FVNSYTPDIVTIEPGAFAGKVTKVLKGNRDTGLAAGEFNFQMKITPADDTSSMDNVVL
                     PEGAVGDTITSANAADGSVSFGNIQFKAAGNYHVEITEVVPEYADPNMTYDKHTFSYD
                     IAVTYNAAEGTLSAKVADGSQAGSATFTNIYEAEDTKDVINTEEPATAVNGKIVGVGD
                     QLTYTIDWVNNAVDETGAPAKAEVKITDIVPKGTEYVSADTNGVYEETSKTITWTLGE
                     QEAGAFGTVSFVVKVLDSAGGTAVENTAEITIGDNDPNQTNTVTTNVPGKDSVVEGDG
                     ELQVGKVLTYTISYKNPEIEAATVIITDSIPEGLDYVDDSAGEYASYNAETRMLTWKI
                     ADVQPGADGTVTFDARVNESAETVVENKATIQIGENGPEYETDTDRKEIPKDGNLSIS
                     KTIVLTEGQGTGIDKEKEFTFIVALKDAKGTALTKEYAYAVTGESGEEIKKGNAADGT
                     EISFQHGQKAVITGLPAGAQYTVTEKEVDGYTTTVNGNAGNAASGTITSNTTAEANFT
                     NTYSVAGSLSGSEALKVTKELTGRKWLDTDTFSYTLTAADEATVEAIENGFITLPENA
                     GGLEITKASAGHQAAFGDIQFTIAGTFKFNVTEQPSGIAGITDDQEAERTVVVKVTDK
                     KNGTLEAAVVEGESENLTFINTYGAGTGDEDIAAKIEASKKLTGRDMKANEFRFEIVT
                     RAAENPEGFKETFAAAGTNTEAAEGAEGAVKFAGNDGALTYTTESLDQAVKDGYAVKS
                     ENDDGSDVWTVSYTAREITDKLPGGVTAVEGKTSFDFTVVVTDRTDGTLTAQVQLPEG
                     GIVFENAYTAEDTEVNTDPADAKSYFNKVLTGRGWLGTDEFTFTITPQDGAPAAEDAA
                     ADGTKTVTVTSDSAKAGEAVLFGFGKIRFTDEDMNGAAAGQDGTRTKEFRYTVKENPL
                     PDGKMTGVTIDGHEATLKITVSDNLEGKLEVTNIASENGTFTNEYKSQLDYAAAGGLQ
                     ITKALNGRDMEKDQFTFTVTPKATEGSTTAEEAAEKFGLANAANTYKNEAAEAGAVTT
                     INVLDGKNVTFTQADAGKTFTYEAAETAGTNTAYTYDTDVRTVTVKVKDNNNSTLTVT
                     TRVTKVKDGQEVIVDEQEVTTGEVGQKKATISFVNTYNDEPVELGGEGSVKINATKTL
                     ANRPLTDGEFTFRIFDKKGNPVVGKDAEATGTNAADGSITFAPVSYDTDRLMADVKAG
                     IASVDKKSQAPAYVYTYDYTVTEDNPSGGVTGIATTFAIQVIVTDRGDGTLAIEVKYP
                     DGKDSLPFENVYGKSANAEIALNGKKVYEKESVNAPDIAGKYTFTITGTDELGNPAPM
                     PVKDGKVVTETTNDAAGNIDFGKLVYTMENVFGSAESQDEQTNANQDVEAGEDPADTN
                     QNAEAGQDPAAADTAEDKTAAEDAADAAENAASEETAGDGTEGTKAPETDETDIEANG
                     GSNAGSSGEDQDKEAAGAASADGGNVVDQAGTMNAQEAGQANRFSEPRTKVYTYRVTE
                     SGSMAGVTNDLAAAEGKTFTVTVTDNGDGTISAVSSWQNDFAFEFTNTYRVNPTDYSV
                     NEHISIKKELTGRDLHEGEFTFELIDESGNVVASAVNEADGTVRFGALHYTEAGTYNY
                     VIREVEGTAGGVQYDSAEHTVTVIVTDNGDGTLSAKAETKSGEDMNGIVFRNIYEARP
                     ASVTLGASKTYKGAELKDKQFTFVLKDKDGNVVFEAKNGADGQVMFETLIYDRAGVYE
                     YTISEKNDGQKNVTYDETVYNVTITVTDNGKGNLIAEAVYGDGRTPQFVNTYVKPQNP
                     VKPEQPTPTVKPQNPGAVQTGDHNSFAGTLGMAALALAVAAGVMIRRKKKS"
     gene            10575..12044
                     /locus_tag="ACZPD4_01320"
     CDS             10575..12044
                     /locus_tag="ACZPD4_01320"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1377753.1"
                     /translation="MEHLYRKLKEYADTGYYPFHMPGHKRNTALIGSELPYELDITEI
                     DGFDDLHHSEGILKELQEYAACVYHAEETHYLVNGSTVGLLSAVMGSTNSGDCILMAR
                     NCHKSVYNAVFMNQLHPIYIYPENGAINPGEVKRILEENPNVKIVVITSPTYEGVVSD
                     VERIAGIVHEKGIPLIMDEAHGAHFGFHPYFPKNSNTLGADIVIHSLHKTLPSLTQTA
                     LLHMNGELANRTDIREYVHMLQSSSPSYILMAGIDECVRFVGERSSDVFDNYVKRLES
                     VRKRISRLSVLKLIETEKYDRSKIVTAVGNHGDEDINRFTGKQLYDVLKDKYLLQPEM
                     ASGSYVVLMTSPADTDEGMERLVSALEEIDGELAKRDKYERNNSLARYIVQDSDTYQY
                     ILKDKKERDHQKNIYIYSPSETKRQRLKKGCESVEWKMCGGKVAAEYAYIYPPGIPLI
                     VPGERISEKIAEEMSEYEKMGFRIEGTKVKGKIEVLING"
     gene            12037..12621
                     /locus_tag="ACZPD4_01325"
     CDS             12037..12621
                     /locus_tag="ACZPD4_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1377754.1"
                     /translation="MGKIYCVMGKSSSGKDTVYKKLKEQYKEFRLIVPYTTRPIREGE
                     KDGVEYYFVDPEQFRAMKEDGKVIESRSYNTKCGIWTYFTADDGQIDLSAADYLLIGT
                     LVSYQALREYFGEEAIVPVYLEVEDGLRLARALERERRQEKPKYAEMCRRFLADEEDF
                     SEENLIKSGITERFGNEDFTECLNKIQRYIEEIR"
     gene            12764..13759
                     /gene="holB"
                     /locus_tag="ACZPD4_01330"
     CDS             12764..13759
                     /gene="holB"
                     /locus_tag="ACZPD4_01330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR1377755.1"
                     /translation="MSGFKDVVGHKNIIKYIQNAVTADAVSHAYILNGERGSGKKLLA
                     NLFAMSLQCENRDEDGDACGKCRSCRQAAGGNQPDIIRIMHEKPNTIGVGDIRTQVND
                     DIMIRPYSSKYKIYIIADADMMSVEAQNALLKTIEEPPEYAVIMLLTENAETLLPTIR
                     SRCVMMKLRNIKDQLVKKYLMEQLEVPDYKADVCVAFAQGNMGKAIMLANSEYFNEIK
                     EEVVHLLRNIDEMTVPDLMEAVKRCMIYKMEISDYLDMIAIWYRDVLIYKATRSVDRV
                     VFSDQMKYIRERAKKSSYEGIENILDSLEKAKARMKANVNFELTMELLLLTIKEN"
     gene            13764..14642
                     /locus_tag="ACZPD4_01335"
     CDS             13764..14642
                     /locus_tag="ACZPD4_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR1377756.1"
                     /translation="MVKVIGVRFRTAGKIYFFSPGQFDIKQGDNVIVETARGVEFGRV
                     VSGVKEVAEEDVIQPLKSVIRIATEQDQKVVNKNKQKEKEAFKICLEKIRKHGLEMKL
                     IDVEYTFDGNKILFYFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDETKIRGGIGI
                     CGRPLCCSTYLTEFSAVSIKMAKEQNLSLNPTKISGVCGRLMCCLTNEEETYEMLNSQ
                     LPSVGDAVTTKDGLTGVVHSLSVLRRLVKVIVNLENDEKEIREYMADELKFKPRRKKQ
                     KVTKEEMKQLAELEKE"
     gene            14653..15366
                     /locus_tag="ACZPD4_01340"
     CDS             14653..15366
                     /locus_tag="ACZPD4_01340"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1377757.1"
                     /translation="MSGQKKGKEAVLFPGERLDDLQLNGLELIQDPKKFCFGVDAVFL
                     SDFVKIKAGERALDLGTGNGIIPILLSEKTQGRHFTGLEIQPEMAEMARRSVDYNGLE
                     DKVDIVTGDIKEAAEIFKPAFFDVITTNPPYMIADHGLRNPADAKAIARHEVLCSLDD
                     ILRESMRLLQDKGRFYMIHRPFRLTEIMIKMNYYKIEPKRIQFIYPYLDKEPTMVMIE
                     GVRGAKPRITVEPPLVIYK"
     gene            15508..16368
                     /gene="rsmI"
                     /locus_tag="ACZPD4_01345"
     CDS             15508..16368
                     /gene="rsmI"
                     /locus_tag="ACZPD4_01345"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1377758.1"
                     /translation="MAGTLYLCATPIGNLEDITFRVLRVLREADLIAAEDTRNSIRLL
                     NYFEIETPMTSYHEYNKFDKGRVLIEKLLEGKDIALITDAGTPGISDPGEELVRMCYE
                     EGITVTAVPGASACVSALTISGLPTRRFAFEAFLPSDKKEREAVLSCLEKEQRTIVLY
                     EAPHRLIKTLKMLGERLGDRRVSVCRELTKRHETVYRSTLLEAVSYYEENLPKGECVL
                     VIEGLSREEIEQEEQKKWSNLSIEEHMEYYLSQGIDKKEAMKKTAKDRGVQKREIYNY
                     LEKLKSGEGN"
     gene            complement(16389..>16615)
                     /locus_tag="ACZPD4_01350"
     CDS             complement(16389..>16615)
                     /locus_tag="ACZPD4_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377759.1"
                     /translation="ALGRAIVCQPKVLLVDEDFARQDPERRKEMLCDILEINEELKIT
                     VLYVTNDPLEARTLGKKIVFMKDGKITDIG"
BASE COUNT         5506 a         2948 c         4523 g         3638 t
ORIGIN      
        1 tacatagatc cgaatggaaa gacattgggc aatacgactg cttctttcca accggcaaag
       61 aaaaatacat tttattattt tacagaagat actccgattt atacagataa agattgtaca
      121 atggcagtaa agaacgagcc gagtgcggat cagacgtatt attataagag accgtatgtg
      181 cagttaaacg gaggtcaggt tgaagaaatc gacggcaagg tagcctttac gggatcaaac
      241 ttccagcagg caagtgcgaa cttcggtata aatgacaagg gagaatactt tatcaagtcc
      301 ggttctgcac gattgacaag aatcgatgcg ttgatagatg ataaaacaga gaacataaca
      361 aatacggcgg atgtagtgat cgatccgatt tgggataata tcaataatcc cgattactta
      421 aatgtttatc tgggaaataa cggaaggctt gcgcttgagc ttccgggcgc tcttgcaatc
      481 agcaaagatg cccgcgtggc agcgaataaa aatctgaatg cggatgaaat attaaaagat
      541 aaaaagttca catttgagat cagtattccg tctatgaagg gaaaaacagt caaagcggaa
      601 gtacgaaatg cgcaaaatga aattcaggga aaagcatttg aactggaatt tgataagacc
      661 ggagctgcca agtatcagat taaagatgac gaaacgctgt atattcatgg attggataaa
      721 gatgcgcaat ataccgtaac cgaaaaagaa ctgccgaaag gatttgaact gacggcagtt
      781 gataaaaagg cggacgcaaa agaggcggct ggaacgatcg tttcaggaaa atctgtaaaa
      841 catgtgtttg aaaatacata tgacgtagat gaaatcacgc ttgccgaaac tgatttcgca
      901 agttatgaga aacagtttga ccggtgggat attgcagata agttccgaat cgaattgatt
      961 ccgacaacag aaggagctcc gcttccgaat ggagctgctg atggtaaaaa aggcgttgag
     1021 gtaacagaaa aaaattcatc aggaaacttc ggagagatca cattttctca tcccggagta
     1081 tatgaatatg aaattcagga gaaacagccg gcaagtgcaa taccgggagt tatatattct
     1141 ctggcatcct atacatatcg ggtaacggtg accgataatg gagacggaac attatccgct
     1201 gtggcagaaa tggaaaaaac ggctaatgac gacggggcgt cagtaggaaa tacgccgatt
     1261 ccggttgaga ataaaacagc ggtatttgta aatgatttcc atgcagattc cgcaacgaca
     1321 tctatacttg caaaaaaagt gtatgcagat gaaagcggag cgaatccgtt gaaaaacgga
     1381 atgtttgaat ttaaactcaa agcgaccgga gacaacgcag agcaggcgcc aatgccgacc
     1441 ggagagaagg atgaaaatgg ttatatccat gtagtcaacg tcggaacagg aattacattc
     1501 ggtaacatgg tgtttacaga ggagaatgtc agtgatacac cgtggacgta tgaaattgca
     1561 gaagtgattc cggaaactgc ggttaataat ggagacggaa cttatacttt aaacggtata
     1621 acttataagg cacaaacata ttccgtatct attatggcaa aggcacaagg ttcaggcgag
     1681 gatgcgtata ttgtcataga aaagacatac agcaaagcag aaggagcagt tgctgaagat
     1741 caggttgtat tcaacaacag ttatgaaccg aagcctatag tacttccggg tgagggagaa
     1801 tcagcggtac agggaagaaa aactttagtc ggacgcgatt ctcttgtgga tgaagagttc
     1861 tcctttacat tgagtgctgc aaataccgca gcaagaaccg gtcttaaaga aaatttcatt
     1921 atcttcaatg gagatacggc tcaggatacg atgcaaaaaa cggtgaacgg actgacgaat
     1981 aatcaggctg caacatttga ttttgacagt atcgagttca aacgtccggg aacatatgta
     2041 tttagtgtag tagaaaatgc cccggaaaac ggaaacggaa tggtatatga ccgacataca
     2101 gcaagagtac gggttattgt gacagatgag aacggagaat taaaggcaga aacagcttac
     2161 tataacggtg aaggcgcaga tactgatgcg gctgcatttg tcaatctgta tacagcgtct
     2221 gcaacttacg gaacaggcgc ggaactgatt gtagaaaaga cgttatccgg ccgtgctctg
     2281 aaagcgggcg aatttacatt taaaatcgag gcggcagaca gtgatactgt aaatgctgag
     2341 acagcggatg ctaaattgtc agattctgac agagaattta caaatacatc aggagcggca
     2401 gacggtgttg catctcggat ctttgacaaa ttgtcagggg tgacctttac acaggatgat
     2461 gcaggaaaga cgttcagcta cgttctttca gaggcagcag gaaatttacc gggagttaca
     2521 tactcaaagg acacatatcg ctttgatatt acggtagtag ataacggcga cggaacgatg
     2581 catacagaaa cgaaagttac aatgcaggga gatggtgtga cagcagaagc tcatcatgat
     2641 agttcagacg gaagagatac gatagtagca ggttttacga acggatatca tgcagacagc
     2701 gtggaagtag acttcacaga agcggcagca ttgttccaca aacgactggt tggacgcgac
     2761 tggaaagaag atgattcgtt tacattcaat cttacggcag aagatggaac tccaatgccg
     2821 aaagatacgg aaggcaatga tgtaacaagc gttactgtat cacagaaagg cggaacggaa
     2881 gatggtacgg atgtaccgtt tggattcgga aaggtcacat atgatacagt cggaaagtat
     2941 tcctacactg ttacagaaga gaatgcagga caaaccatta acggagtgac ttatagtaag
     3001 aatgaggcaa aaataatagt tgatgtttct gatccgggaa atgggcagtt gaccgcaaat
     3061 atacagtcct atagtacgat atttattaat gaatacggcg cttctctgaa cctcggagcg
     3121 gcaggcggac ttgcaattac aaaaacagtg acaggtcatg cattagcgaa agagcagttt
     3181 gaatttaagg taaaagcggt taagacagac actgctacag cagatgaagc ggcagaactc
     3241 tttggattta aagagggaga gacagaggtt acatttaaga acaacgaagc ggcggcagat
     3301 ggacaaactg taaccatgtt aaaaacagat acgaacttta cgttgaagaa tctgggtaaa
     3361 gtatacaaat atgaagtatc ggaaatgaat gataagaaac cgggatatac atacgatgat
     3421 actgtttata cagtagaatt atgggtaaca gatgatggag acggaacatt aacgcttcat
     3481 acaaaggtga cagatgaaaa aggcagtgtc ataagcgaag agacaagcaa tgaaacagat
     3541 caaaagcaga ctgttctcgc attcaacaac agctatgccg gaagcggtgt tcttgacgga
     3601 tcagcaaatc ttgcaggaac aaagaagatg gatggtccat gggaagcaac cgacaaagat
     3661 ttatctggct tccggttcac tatcacgggc ggtgacgaag caacgaatgc ggcaattaca
     3721 gcaggcacag ttgtattacc gcagagtgta acggcaactt ctgatgcaga cggcaacttc
     3781 aactttggag atattacctt taatgagaag ggaacttaca aattcacagt cagtgaagtt
     3841 gtaccggcag agggagacaa gattccggga gttgcctata atgcagagac ggttacgatt
     3901 atcgttaatg ttacggataa tgatgacgga acattaacag cggcgctggc agaaggatca
     3961 caggaattaa tatttacgaa tacttatgca acaacgcagg atgcaacatt tacgccatcc
     4021 gtagtgaagg aggtaaaggg gcttaatgcg aaagagatct ttacattcaa gctgagcgca
     4081 gccgatgaag caacgaaggc ggcgattgac agtggaacac tgaccggaat tggaacgacg
     4141 gcagatctat acagctcaga gaaaacgaca actaaactga ttccaaagga tggaacagag
     4201 caagtagact ttaatgcgct gacatttaag aaagccggaa cttataagtt taccgttcaa
     4261 gagaccaatg caaatgcacc gacaggatgg acgtatgatt ctcatacata tgagattatt
     4321 attaaagtaa cagaccagga ttctgtattg aaggcaacac aagagatcaa tgctgacggg
     4381 gtgaccaatt cacaaatctt catcaataaa tttgaagcat cgacaactta cggtgatgaa
     4441 ggcggactga atgttacaaa gacattgaat ggacgtacac ttgcagcaga tatgtttgat
     4501 ttcacaatta ccggtgaagc aactgacagt gtaactgcag aagaagcaga agcaaaactt
     4561 gcagagactg ataagagctt taagaatact gcaccaggca aagacgatgt cgcggtgatg
     4621 agtaagctta gtgacgtgaa gtttgatgaa acagacattg gtaagacata tcaatactcc
     4681 gttcgcgagg cagcaggcac cgatacaaag tatacgtatg atcaagtatc agcgaccgtt
     4741 gcaattacgg tacaggagaa agacggagag ctgtatacgg taacaaccgt aaccaaggga
     4801 gacgatagta aggaatatag ttcggcagaa agtaaagcta cagcagtagc gccgttcgta
     4861 aacagctata caccggatat cgtaaccatt gagccgggag catttgccgg aaaggtaacg
     4921 aaagttctga aaggaaaccg tgacacaggt ttagcggcag gtgaattcaa tttccaaatg
     4981 aagattacac cggcagatga tacaagcagc atggacaatg ttgtacttcc ggagggagca
     5041 gttggagata caatcacatc ggcaaacgcg gcagacggtt ctgtaagctt cggaaatatt
     5101 cagttcaaag cggcgggaaa ctatcatgta gagattacgg aagtagttcc ggaatatgct
     5161 gatccgaata tgacgtatga caagcatacc tttagctatg acattgcagt gacttataat
     5221 gcggcggagg gaacgttgtc agcgaaagtc gcggatggaa gtcaagcagg aagcgcgaca
     5281 tttactaata tttacgaggc ggaagacacg aaggatgtta ttaacacaga agagccggcc
     5341 acggccgtaa atggcaagat tgtcggtgtc ggcgatcagt tgacttatac tatcgactgg
     5401 gtaaataatg cagtagatga aacaggcgct cctgcgaaag cggaggtgaa gatcacagat
     5461 attgtaccga aaggtacgga atatgtaagc gcagatacga atggagttta tgaagagacc
     5521 tcaaagacaa tcacctggac gcttggcgag caggaagcag gagctttcgg aacggtaagc
     5581 tttgtagtaa aagtgcttga cagcgcaggc ggaacagcgg tagaaaatac ggcagagatt
     5641 accattggag acaacgatcc aaatcagacc aatacagtta ccacgaacgt tccgggcaaa
     5701 gattctgtag tagaaggcga cggagaatta caggtcggca aggttttgac ctataccatt
     5761 tcctataaga acccggaaat tgaagcggcg acagttatca ttacagacag cattccggaa
     5821 ggtcttgatt atgtagacga cagcgcgggc gaatatgcat cgtataacgc agagacacgt
     5881 atgctgacat ggaagattgc agatgttcag ccaggagcag acggaacggt tacatttgat
     5941 gctagagtaa atgaaagcgc cgagacagta gttgagaaca aagcgacgat ccagatcggc
     6001 gaaaatggac ctgaatatga gacggatacc gatcggaaag agattccgaa agatggaaat
     6061 ctttccatca gtaaaaccat cgtactgaca gaaggtcagg gcacagggat cgacaaagag
     6121 aaagagttta catttatagt agcgcttaag gatgccaaag gtacggcatt gacgaaagaa
     6181 tatgcttacg ccgtaacagg cgaaagcgga gaggaaatca agaaaggtaa tgctgcagac
     6241 ggtaccgaga tttccttcca gcatggacag aaggcagtga ttaccggact tccggctggc
     6301 gcgcagtata cggtaactga aaaagaagtt gacggataca ccacaactgt aaacggaaac
     6361 gccggcaatg cggcaagcgg tacgattact tcaaatacaa ctgcagaagc aaactttacg
     6421 aatacgtaca gcgtggcagg aagtcttagc ggaagcgaag cgcttaaagt gacgaaggaa
     6481 ctgacaggaa gaaaatggct tgatacagat acgtttagct atacgttgac ggcagcggat
     6541 gaagcgacag tggaagccat tgagaatgga tttatcacgc ttccggaaaa tgcaggcgga
     6601 cttgagatta caaaagcatc tgcaggacat caggcggcat ttggagatat ccaatttacg
     6661 atcgccggaa cgttcaagtt caatgttacg gagcagccaa gcggtatcgc cggtattacg
     6721 gacgatcagg aagcggaaag aactgtggtt gtgaaagtga cagacaagaa aaacggaacg
     6781 ctcgaagcag ccgttgtcga aggcgagagt gagaatctga cctttatcaa tacatatggc
     6841 gcaggcacag gagacgagga tatcgcggcg aagatcgagg cgtcgaagaa actgaccgga
     6901 cgtgatatga aggcgaatga gttccgattt gaaattgtga caagagcggc agaaaatccg
     6961 gaaggattca aggaaacttt cgcagcggca ggaacgaata cagaagctgc ggaaggagcg
     7021 gaaggagcag taaaatttgc aggaaatgac ggcgctctga cgtatacgac ggagtcgctt
     7081 gatcaggcag taaaagacgg atatgccgta aaatctgaaa atgatgacgg ttcggatgta
     7141 tggacggtca gctataccgc aagagagata acagacaaac ttccgggcgg agtaaccgca
     7201 gtagaaggaa agacgtcttt cgactttacg gtcgtagtga cagatcgtac agatggaaca
     7261 ttaactgcac aagtacagct tccggaaggc ggtattgtat ttgaaaatgc ttatactgcc
     7321 gaagacacag aggtcaatac agatcctgcg gacgcaaaat catactttaa taaagtgctg
     7381 acagggcgcg gttggttagg aacagacgag tttacattta ccattacgcc gcaggatggc
     7441 gcgccggcgg cggaagacgc ggcggcagac ggtacgaaaa cagtaacagt aacgtctgac
     7501 agtgcaaaag caggagaagc tgttctgttt ggtttcggaa agatccggtt tacagatgag
     7561 gatatgaacg gagctgccgc aggacaggat ggaacaagga cgaaagaatt ccgttacaca
     7621 gtgaaagaaa atccgcttcc ggatggaaag atgacgggag tgacgatcga cggacatgag
     7681 gcgacattga agatcacagt atcggataac ctggaaggta aattggaagt aacaaacatc
     7741 gcctccgaaa acggaacatt tacaaatgag tacaaatccc agcttgatta tgcggcggca
     7801 ggaggactgc agatcacgaa ggcgctgaac ggaagagata tggaaaaaga tcagttcacc
     7861 tttacagtga cgccgaaagc gacggaagga agcacgactg cggaagaagc ggcggaaaaa
     7921 ttcggacttg cgaatgcggc aaatacatat aagaatgaag ccgcagaagc aggcgcagtg
     7981 acgacgatca acgtactcga cggaaagaac gttacattta cgcaggcaga cgcaggcaag
     8041 acgtttacct atgaagcggc ggagacggcg ggaacaaata cagcttatac gtatgataca
     8101 gatgtaagaa ccgtaacagt caaagtaaaa gataataaca attcgacgct gacggtaacg
     8161 acacgggtta cgaaagtaaa agacggtcag gaagtgatcg tggacgagca ggaagtaaca
     8221 acaggagaag tcggtcagaa aaaagcgact atatcgtttg taaatacata caacgacgaa
     8281 ccggtcgagc ttggcggaga aggaagcgtg aagatcaacg cgacaaagac gctggcaaac
     8341 agaccgctga cagacggaga atttacgttt agaatatttg ataaaaaagg aaatccggtt
     8401 gtcggcaaag acgccgaggc gacaggtacg aacgcggcgg acggaagcat tacgtttgct
     8461 ccggtaagct acgatacaga cagactaatg gcagatgtga aagcaggaat cgcatccgta
     8521 gacaaaaagt ctcaggctcc ggcatatgtc tacacatatg actatacggt gacggaagac
     8581 aacccgtccg gcggagtgac aggtattgcg acgacatttg cgatccaggt gatcgttaca
     8641 gaccgcggcg acggaacgct ggcgatcgaa gtgaaatatc ctgacggaaa agattcgctt
     8701 ccgtttgaga atgtatacgg caagtctgca aatgcagaga tcgcgctgaa cggaaagaaa
     8761 gtatatgaga aagaatccgt caacgcgccg gatatagcag gaaagtatac atttacgatc
     8821 acaggaacag atgaacttgg aaatcctgcg ccgatgcctg taaaagacgg caaagtcgtg
     8881 acagagacga cgaacgatgc ggcaggaaat atcgatttcg gaaaacttgt ctatacgatg
     8941 gaaaatgtat ttggttcagc ggaaagtcag gatgagcaga caaatgcaaa tcaggatgtg
     9001 gaagcaggag aagacccggc ggatacaaat cagaatgcgg aagcaggaca agacccggcg
     9061 gcagccgata cagcggaaga taagacggcg gcagaagatg cggcagacgc ggcagaaaac
     9121 gcggcgtcag aagaaacggc gggagacgga acagaaggaa cgaaagcgcc ggaaacagat
     9181 gaaacagaca ttgaagcaaa cggcggatcg aacgccggat caagcggaga agatcaggac
     9241 aaagaagcgg caggagcggc ttcggcggac ggcggaaacg tcgttgatca agccggaaca
     9301 atgaacgcgc aagaagcagg tcaggcgaac agattcagcg agcctcggac aaaagtttat
     9361 acttatagag tgacagagtc cggcagcatg gcgggcgtga cgaacgattt ggcggctgcg
     9421 gaaggaaaga cgtttacagt aaccgtgaca gacaacggcg acggaacgat cagcgccgtt
     9481 tcctcatggc agaatgattt cgcatttgag tttacaaaca cataccgtgt aaatccgaca
     9541 gattacagtg tgaatgaaca tatcagcatc aaaaaagagc tgactggacg tgatctgcac
     9601 gagggagaat ttacatttga actcatcgat gagtccggaa atgttgtggc ttcggcagtg
     9661 aatgaggcgg acggaacggt tcgtttcgga gctctgcatt acacagaagc aggcacatac
     9721 aattatgtga tccgcgaggt ggaaggtaca gccggaggcg tacaatacga ttcggcagag
     9781 catacggtga ctgtcattgt gacagataac ggcgacggta cattgagcgc gaaagcagag
     9841 acgaagagcg gagaagatat gaacggcatc gtattccgaa atatctacga agctcgtccg
     9901 gcgtcggtaa cgctcggagc ttccaagact tataagggag cggagctgaa agacaaacag
     9961 tttacgttcg tgctcaaaga caaggatgga aacgttgtgt ttgaagcgaa gaacggcgca
    10021 gacggacagg ttatgtttga gacgctgatc tatgaccggg cgggcgtata tgaatacaca
    10081 atttctgaga aaaatgatgg gcagaaaaat gtaacatacg acgaaaccgt atacaatgta
    10141 acgattacgg ttacagacaa cggaaaaggc aatttgatcg cagaggcggt atacggagac
    10201 ggcaggacgc cgcaattcgt taatacatat gtgaaacccc aaaatccggt gaaaccggaa
    10261 cagccgaccc cgactgtaaa accacagaat ccgggagcgg tgcagaccgg agatcataat
    10321 tcgtttgcgg gaacgctggg aatggcggcg cttgctttag cggtggcagc aggtgttatg
    10381 atcagaagaa agaaaaaatc ataaaataac gtgattttgg aaatgagagc agcggcagag
    10441 gatgccgctg cttttttgta tatagccata gtcaatcata aagctctgtg ttagaataaa
    10501 gcagtgatat gacgatgtaa agaaacaaga gaaaagaacc gatgtacaga ggacagggtg
    10561 accggaggga tatgatggag catttatata gaaaattaaa ggaatatgcg gacacgggtt
    10621 attatccgtt tcatatgccg gggcataaaa ggaatactgc gctgattgga tcagaacttc
    10681 catatgaact ggatatcaca gaaatcgacg gatttgacga tcttcatcat tcggagggga
    10741 tactgaagga actgcaggag tatgcggcgt gcgtatatca tgcggaggaa acgcattacc
    10801 ttgtgaacgg gagcacagtc gggctgctga gtgcggttat gggaagcaca aactcgggag
    10861 attgtatttt aatggcaaga aactgtcata aatcggtcta taacgcagtc tttatgaacc
    10921 aacttcatcc gatttatatt tatcctgaaa acggagcaat aaatcccggt gaagttaagc
    10981 ggattcttga ggaaaatccg aatgtgaaga ttgttgtcat tacatctccg acgtatgaag
    11041 gcgttgtttc ggatgttgag cggattgccg gaattgttca tgaaaagggc attccattga
    11101 tcatggatga ggcgcatggg gcacatttcg ggttccatcc gtattttcca aagaacagta
    11161 atactttagg ggcagatatc gttattcata gtctgcacaa gacgcttccg tcgttgactc
    11221 agactgcgct gcttcatatg aatggagagc tggctaacag aacggatatc agagagtacg
    11281 tccatatgct tcaatcgagc agtccgtcat atatacttat ggcaggaata gacgagtgtg
    11341 ttcgtttcgt gggagagaga agctcagatg tttttgacaa ttatgtgaag cgtctggaat
    11401 ctgtaagaaa gagaataagc aggctttctg tgttgaaact gattgagacg gagaagtatg
    11461 acaggtcgaa gatagtaact gcggttggga atcatggcga tgaagacata aataggttta
    11521 caggaaaaca attatatgac gttttgaaag ataaatattt actacaaccg gagatggcat
    11581 caggctcata cgttgttttg atgacttcgc cggcagatac agacgaaggg atggagcgcc
    11641 ttgtgtctgc attggaagaa attgacggcg aacttgcaaa aagagataag tatgaaagaa
    11701 ataattctct ggcgcgatat atagtgcagg attcagatac atatcaatat atattgaagg
    11761 acaagaaaga acgggatcat cagaagaata tatatatcta tagtccgagc gagacgaaac
    11821 gacagcgttt aaaaaaggga tgcgaatctg ttgaatggaa aatgtgcggc ggaaaggtcg
    11881 cggcagaata tgcctatatt tatccaccgg ggatcccgct gatcgttccg ggagaacgga
    11941 tctcagagaa aatagcggaa gagatgtccg agtatgaaaa aatgggattt cggattgagg
    12001 gaacaaaggt gaaaggaaag attgaggtat tgataaatgg gtaaaattta ttgtgtgatg
    12061 ggaaagagtt cttcgggaaa ggatactgtt tataaaaagt taaaagagca atataaagag
    12121 tttcgtttga ttgttcccta tacgacccgt ccgatcagag aaggcgaaaa agacggcgtc
    12181 gaatattatt ttgtggatcc ggaacagttt cgtgcgatga aggaggacgg gaaggtgata
    12241 gagtcccggt cttataacac aaagtgcgga atatggactt attttactgc ggatgacggg
    12301 cagatcgatc taagcgccgc agattatctt ttgatcggaa cgctcgtgtc atatcaggct
    12361 ttgcgggaat atttcggaga ggaggcgatc gtgccggttt atctcgaagt ggaagacgga
    12421 ctgcggcttg cccgcgcgct tgagcgggaa cgcagacagg agaagccgaa atatgcggag
    12481 atgtgccgcc gttttcttgc ggacgaggag gacttttcgg aagaaaatct tatcaaaagc
    12541 gggattacag agcggtttgg caatgaagat ttcacagagt gtctgaataa aatacaaaga
    12601 tatattgaag aaataagata gattttatca gtttttatga gatttcactc ttttgtaata
    12661 ccttttaatt taaaattgta gtacaatagg agtatgtctt gaaagggaaa cgtcttctga
    12721 gaggcatttt ggaaaattaa ttttaagagt gcaggtgata tggatgagtg gttttaaaga
    12781 cgtagtagga cataagaata ttattaagta cattcagaac gcagtgactg cggacgcagt
    12841 ttctcatgcg tatattttga atggcgagag aggttcaggg aagaagctgc ttgcgaattt
    12901 gtttgccatg agcctgcaat gtgaaaaccg ggatgaagac ggcgacgcct gtggaaaatg
    12961 ccgttcgtgc aggcaggcgg caggcggaaa tcagccggat attatccgga tcatgcatga
    13021 gaaaccgaat acgatcggag taggagatat ccgtacgcag gtgaatgacg atattatgat
    13081 ccggccgtac agcagcaaat ataaaatcta tattatagca gatgcggata tgatgagcgt
    13141 ggaggcgcag aacgcgcttt tgaaaacaat cgaagaaccg ccggagtatg ccgttatcat
    13201 gcttctgacg gagaatgcgg agacacttct tccgacgatc cgttcaagat gtgttatgat
    13261 gaagcttcgc aatattaaag atcagctcgt gaaaaaatat ctcatggagc agttggaagt
    13321 gccggattat aaagcagatg tctgcgttgc ttttgctcag ggaaatatgg ggaaggcgat
    13381 catgttggca aactccgaat atttcaatga gattaaagag gaagttgtac atttgctgag
    13441 aaatatcgac gagatgacag ttccggatct gatggaagcg gtaaaacgct gcatgatata
    13501 taagatggag atcagcgatt atcttgatat gatcgcgata tggtacagag atgtgttgat
    13561 ctataaagca acaagaagtg tggatcgggt cgtgttttcc gatcagatga aatatattcg
    13621 ggagcgggcg aagaagagct catacgaggg gattgaaaat attctcgatt ctcttgaaaa
    13681 ggcaaaggca agaatgaaag cgaatgtaaa ttttgagctg acgatggaac tgctcctgct
    13741 gacgataaag gagaattgac aaaatggtaa aagtaatcgg agtcagattt cgtactgccg
    13801 ggaaaattta ttttttttct cccggacagt ttgatattaa gcaaggcgac aatgtgatcg
    13861 tggaaactgc ccgcggcgta gagttcgggc gagttgtaag cggagtaaaa gaagttgccg
    13921 aagaagatgt gatccagccg ttgaaatcag tgatcaggat tgcgactgag caggatcaga
    13981 aagttgtaaa taaaaacaaa cagaaagaaa aagaggcgtt taagatctgc cttgaaaaaa
    14041 tccgtaagca tggactggaa atgaaattaa tcgacgttga gtacactttt gatgggaata
    14101 agatattatt ttatttcaca gcagacggaa gaattgattt tagagagctt gtaaaagatc
    14161 ttgccgcagt atttcgcaca aggatcgaac ttcgtcagat cggagtgcgt gacgagacga
    14221 agatccgcgg ggggatcggt atctgcgggc ggccgctttg ctgcagcaca tatttgactg
    14281 aattttccgc tgtgtcgatt aagatggcga aagaacagaa tctgtctctg aatccgacga
    14341 agatatcagg tgtgtgcgga aggcttatgt gttgtctgac gaatgaagaa gagacgtatg
    14401 agatgcttaa cagccagctt ccgtcagtcg gggatgcggt tacgacaaaa gacggactta
    14461 caggagttgt ccattcattg agcgttctgc gcagattggt aaaagtaatc gtaaatttgg
    14521 aaaacgatga gaaagagatc agagaatata tggcggatga gctgaaattt aaaccgagac
    14581 gaaagaaaca gaaagttaca aaagaagaga tgaagcagct tgcggaactt gaaaaggagt
    14641 aggtagcggc atatgagcgg acaaaagaag gggaaagaag cggttctttt tccgggcgag
    14701 cgactggatg atctgcagct taacggtctt gaattgattc aggatccgaa gaaattctgt
    14761 tttggcgtag atgctgtttt tctttcggat tttgtgaaaa taaaggcggg ggagcgggct
    14821 cttgatctgg gaacagggaa cgggattata ccgatactgt tgtctgaaaa gacgcaggga
    14881 cgtcatttta caggtctgga gattcagccg gagatggcag agatggctcg aagaagtgta
    14941 gactataacg gtttagaaga taaagtggat attgtgacag gcgacatcaa agaggcggcg
    15001 gaaatattta agccggcctt ttttgatgtg atcacgacaa atccgccgta catgatcgca
    15061 gatcacggac ttcgcaatcc ggcggacgcc aaggcgatcg cacggcatga ggtgctttgt
    15121 tcacttgatg atattttgcg ggagagtatg cgccttttgc aggataaggg aagattttat
    15181 atgatacacc gtccattcag gctgacggag attatgatta aaatgaatta ttataaaatt
    15241 gagccgaaac ggattcagtt tatttatccg tatcttgata aagagccgac gatggtaatg
    15301 atagaaggcg tacgcggggc aaagccgcgt atcacggtag aaccgccgct tgtgatttat
    15361 aaataaaaag aaatgaaaag gtaaaataaa taagaattaa aaagatataa aaaagaaatg
    15421 aaaaaacaaa taaagaaata aaaaaataga caacaagaaa agaaaacaga aaacaaaaag
    15481 agacgaagag aaaaacagga gggaaagatg gcaggaactc tctatttgtg tgcgacgccc
    15541 atcgggaatt tagaagatat tacatttcga gttcttcgag tgcttcggga ggcagatctt
    15601 atcgcggcgg aagacacacg taacagcatt aggcttttga attattttga gattgaaacg
    15661 ccgatgacga gctaccatga atacaataaa tttgataaag gaagagtgct tatagagaag
    15721 cttttggaag gaaaagatat tgcgcttatt acagacgcgg gaacgcccgg gatctccgat
    15781 ccgggagaag agcttgtacg tatgtgttat gaagaaggga ttacggttac tgctgttccc
    15841 ggagcttcgg cgtgtgtgtc tgcacttacg atctcgggac ttccgacaag aagatttgcg
    15901 tttgaagctt ttctgccatc agataaaaaa gagcgggaag ctgtcttaag ttgtctggaa
    15961 aaggaacaga ggacaatcgt tttgtatgaa gctccgcacc gtcttatcaa aacattgaag
    16021 atgctgggag agcggctcgg cgacaggcgg gtaagcgtat gccgggaact gacaaaacgg
    16081 catgaaactg tgtatagatc gactcttttg gaagcggttt cttattatga agaaaatctt
    16141 ccgaaagggg agtgcgttct tgtaattgaa ggattgagcc gtgaagagat cgagcaggaa
    16201 gagcaaaaga agtggagtaa tctgtcgatc gaagaacata tggagtatta tctgtcgcag
    16261 ggaatcgata aaaaagaagc gatgaagaaa accgcaaaag atcgaggagt gcagaaaaga
    16321 gaaatttata actatcttga aaaactaaaa agtggtgaag gaaactaaaa aataaccgat
    16381 accggaggtt atccgatatc ggttattttg ccgtctttca taaatacgat ctttttgcct
    16441 aatgtgcggg cttccaaagg atcgtttgtt acatataaga cggtaatttt aagttcttcg
    16501 ttaatttcga gaatatcaca aagcatctct tttcttcgtt caggatcttg acgcgcaaaa
    16561 tcttcgtcta cgaggaggac tttcggctgg cagacaatcg cgcgtccgag agcga
//
LOCUS       DBMDXF010000016        16487 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1053_length_16487_cov_2.319907, whole genome shotgun sequence.
ACCESSION   DBMDXF010000016 DBMDXF010000000
VERSION     DBMDXF010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 16487)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16487)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16487
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1053_length_16487_cov_2.319907"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(191..1189)
                     /locus_tag="ACZPD4_01355"
     CDS             complement(191..1189)
                     /locus_tag="ACZPD4_01355"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1377760.1"
                     /translation="MKAEILKMLREADGYISGQEICEKFNVSRTAVWKVIRRLQEDGY
                     EVEAVRNKGYRMVESPDILTKEEVESCMHTEWVGRNVVCYKVTDSTNLRIKQMGDEGA
                     LAGTLAVAEEQTAGRGRRGRSWESPAGAGIFMSVLLRPKITPDKASMLTIVTACSTAK
                     GIKKYFENEGASCPDIQIKWPNDLVVNGKKLAGILTEMSTQIDYINYVTVGIGINANT
                     KKFPEELKDHATSLYLECGHMVKRASVIASIMKCFEEDYRLFLETEDLSGLMETYMSM
                     LVNRDRDVLVLDPSGTYKAKALGIDKNGELIVRREDGTSEHVYAGEVSVRGVYGYV"
     gene            complement(1372..2358)
                     /gene="argF"
                     /locus_tag="ACZPD4_01360"
     CDS             complement(1372..2358)
                     /gene="argF"
                     /locus_tag="ACZPD4_01360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1377761.1"
                     /translation="MDLKGRHFLTLKDFTPEEIIYLLDLAAELKEKKKKGILVDHHRG
                     KNVALIFEKDSTRTRSSFEIAAHDLGMGTTYIGTMGSQFGKKESIKDTARVLGRMYEG
                     IEYRGFGQEIVEELAQYAGVPVWNGLTNEYHPTQMLADMLTIREHFGKLKGLKLVYMG
                     DARYNMGNSLMIACAKLGLDFVACTTEDYFPNEELVEECRVYAKESGSEITLTSDVKE
                     GTKGADVIYTDVWVSMGEPDEVWEKRIRELTPYKVTKEVMENAKPEAIFLHCLPAFHD
                     LGTKVGREIGERFGITDMEVTDEVFESEQSKVFDEAENRMHTIKAVMDATLK"
     gene            complement(2465..3250)
                     /locus_tag="ACZPD4_01365"
     CDS             complement(2465..3250)
                     /locus_tag="ACZPD4_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1377762.1"
                     /translation="MITSTSNIQVKELLRLQKKSREREKEGVFIVEGPRMAEEIPRER
                     IVKLYVSESFQAKCVKEKNDRFIQEAEVMSDTVFAHVSDTKTPQGILAVVRRMEYTAE
                     DILGETPHVLVLENIQDPGNLGTIFRTAEAAGATGIVLSKDCVDLYNPKVIRSTMGAI
                     FRIPFIYTDDLKGTIDELKREGITVYAAHLKGENSYDMEDYKTACAFLIGNEGNGLTE
                     ETAACADRKILIPMEGGAESLNAAVASAVLMFEAGRQRRNKRK"
     gene            complement(3268..3960)
                     /locus_tag="ACZPD4_01370"
                     /pseudo
     CDS             complement(3268..3960)
                     /locus_tag="ACZPD4_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(4072..5520)
                     /locus_tag="ACZPD4_01375"
     CDS             complement(4072..5520)
                     /locus_tag="ACZPD4_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1377763.1"
                     /translation="MEYICRVWGKQIKIIGWFLEMEKKKKSPRRLKFNTVQTVVIGFF
                     GVILGGGILLSLPICNQKPIEFIDALFTSVSSVCVTGLVTIVPAEQFTLLGKIILLLL
                     IQVGGLGVIACMSAFFLLLGKKINMKGRITIQQAYGLDTLSGLVKFVIRILKGTFLVE
                     GIGAVLFSLRFIPEFGFIKGVGYAIFHSVSAFCNAGIDILGSTSFIQYSGSWLINFTT
                     MFLIVMGGLGFPVWHDIAINIKKMTGIKKRPLKWLFTRLCLQSKIVLTMTGLLILCGA
                     VGFFLLEFNNPETMGDMNLLEKMTASLFQSVTTRTAGFATVPQGDLRESSKLLGCMLM
                     FIGGSPAGTAGGVKTTTIAMLLLTVLCVLKNKRDTECFARKIDNNIVRSGITITMITL
                     IFLMSGITALTIFEPGIDFLDLTYEAASAMGTVGLTADLTPILSRGSHVILMILMYVG
                     RIGPLTMALALSGRSDKISKFRELPEENVMLG"
     gene            complement(5656..6348)
                     /locus_tag="ACZPD4_01380"
     CDS             complement(5656..6348)
                     /locus_tag="ACZPD4_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1377764.1"
                     /translation="MSKRILIVEDEESIADLEKDYLELSNFEVEVANDGDTGLQKGLG
                     GDFDLIILDLMLPGVDGFEICRQIRDQKNTPIIMVSAKKDDIDKIRGLGLGADDYMTK
                     PFSPSELVARVKAHLARYERLINSNAEENEVIEIRGLKIDTTARRVWVGGEERSFTTK
                     EFDLLTFLASHPNHVYTKEELFREIWDMESIGDIATVTVHIKKIREKIEYDTSHPQYI
                     ETIWGVGYRFKV"
     gene            complement(6341..7396)
                     /locus_tag="ACZPD4_01385"
     CDS             complement(6341..7396)
                     /locus_tag="ACZPD4_01385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1377765.1"
                     /translation="MKLRTKLIVAFMSVMILPMIFLNVVMHTFASREVGELQQLYMIV
                     VFITTTLLIYWIYRSVSVPLAKLQKAARNIKEGNLDFEIRQESDDEIGQLCQDFEEMR
                     LRLKANAEEKVAFDRENKELISNISHDLKTPITAIKGYVEGIMDGVADTPERMDRYIK
                     TIYNKANEMDLLINELTLYSKIDTNRIPYNFTTISAKGYFGDCGEDLHMELESKGIEF
                     TYCNTMEEDCKVIVDPEQLRRVINNIVSNSLKYMDKPNGKVTMEVKDVGDFIQVELGD
                     NGKGIAAKDLPYIFDRFYRTDASRNSSKGGSGIGLSIVKKIVEEHGGNIWATSEEGAG
                     TTMYFVIRKYQEVSIDE"
     gene            complement(7606..8805)
                     /gene="metK"
                     /locus_tag="ACZPD4_01390"
     CDS             complement(7606..8805)
                     /gene="metK"
                     /locus_tag="ACZPD4_01390"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1377766.1"
                     /translation="MEREKILFTSESVTEGHPDKMCDQISDAILDELMKQDPMSRVAC
                     ETCCTTGVVMVMGEITTKAYVDIPKIVRDTVREIGYTRGKYGFDADTCGVITTIDEQS
                     SDIAMGVDKALEAKENKMSEDDIEAIGAGDQGMMFGYASDETEEYMPYPAAMAHKLAR
                     RLTEVRKNGTLPYLRPDGKTQVTVEYDENGVPKRLDAVVLSTQHDPEVTQERIHEDIK
                     KYVFDEVIPANMTDDETKFFINPTGRFVIGGPHGDSGLTGRKIIVDTYGGMARHGGGA
                     FSGKDCTKVDRSAAYAARYAAKNVVAAGLARRCEIQLSYAIGVAQPTSVSVDTFGTGK
                     VSDTKLVEIIRENFDFRPAGIIKMLDLRRPIYKQTAAYGHFGRLDVELPWEKLDKADD
                     LKKYLES"
     regulatory      complement(8984..9081)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9143..10576)
                     /locus_tag="ACZPD4_01395"
     CDS             complement(9143..10576)
                     /locus_tag="ACZPD4_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1377767.1"
                     /translation="MKEKYKKYIGNKAFYMMVMAIAVPIMIQNGLTNFVNLLDNIMVG
                     RLGTEEMSGVSIVNQIIFVYNLCIFGGTAGVGIFTAQYFGQKNDEGIRSTFRYKIWLA
                     LIITVGAAFLLLFFGKQLILLYLTGENGGNEKLALEYGMTYLRIILLDLPAFMILQVY
                     ASTMRECGETVVPMKAGAAAVAVNLIFNYLLIYGKFGFPRLGVAGAAVATVLSRYTET
                     AIVVIWAHTHEKENTYMTGLYKTLSVPRDHVKKYFIKGTPLLLNEALWSFGMAVLAQN
                     YSVRGLTVIAAMNIANTINNLFSVVFAAMGESVAIIVGQALGFGDMKRAREIDNKMIV
                     SCVLISFAVSVFMFLFAPFFPRIYNTTEAVRKAAMGLVLAQAVFIPQNAFLNAAYFTL
                     RAGGKTGVTFFFDCVFLWCVNIPIVFVLCRYTGLAPWAVYSAMQIGEWLKCIAGAVMV
                     KKGVWLENLTGKGEDEKYLLQENEEKH"
     gene            complement(10592..11569)
                     /locus_tag="ACZPD4_01400"
     CDS             complement(10592..11569)
                     /locus_tag="ACZPD4_01400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR1377768.1"
                     /translation="MRRYLSVDCGGTKTAFLLCRETGELEAACTLGPGNYMVNGIDHV
                     LSVLKDGILQICCQAAIAQSEITHSFIAIAGFKDIPADVPVLTRLVRETFPRMSITLG
                     NDTENALAGSLLGKQGIHVIAGTGSIGLGFDKDSYYVRSGGWHHLFGGDEGSGYWIGC
                     QLIRHFTMQADGREEKTMMFDYILEKYGLACPEEILRLVINEWKGERDKIASMSKDAY
                     ELAEQGDSAAAGIFKSAARELAKIVKGVYRNGNFDIPVYVSYSGGVFKAMKYIKETLE
                     EELRDVSCVFTEPCLLPSAGGIILACRAAGNEVSEQMIENLRTAQHMRV"
     gene            complement(11566..12630)
                     /locus_tag="ACZPD4_01405"
     CDS             complement(11566..12630)
                     /locus_tag="ACZPD4_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1377769.1"
                     /translation="MELEKFDPVCAAVLKKTEIEKPAYPVIDFHMHMGKMLLGESYET
                     KYDTKEYVRELQDAGVVCAVNMDGYFGKDLEKMQKKQEGFEEMFFNFMQLDFSAYDDP
                     DFCDKTKKVIEDSCMRGCRGIKLWKDLSLWERDKYGRPIRTDDPRFDIIYDTAAKLHI
                     PVLMHVADPAAFFTPKSEKNERWEELDVCPEWDFSDHEKYMSFEELMEMQENTVRSHP
                     ETTFVIAHVGSYAENLEWVSGQLEKYPNMYIDVAARLAELGRVPYSAKKFFVKHQDRI
                     LFGTDCTPLGMGYHKIYYRFFETKDEYFPYQPEGELPGQGRWAIYGIGLEDEILKKIY
                     YKNACKVLSLDENRFVGGVR"
     gene            complement(12747..13583)
                     /locus_tag="ACZPD4_01410"
     CDS             complement(12747..13583)
                     /locus_tag="ACZPD4_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1377770.1"
                     /translation="MKMLKDKKNRKLRIISFVILFLAAIVFLAPYFWMLSNSFKSTKE
                     ILLEPTNMLPKQFTLDGYVKVITDSPFFRWMGNSLFVTVIDTLVVLFTSSLIGFVLSK
                     YKFKLNKFLFYVILITMMMPTAAMMIPNFLLISKLGWYDKIISLVVPTFVNAFGIFLC
                     KQFIDDMPKELFECAMLDNAGPFTIYRKIVLPGIRPALGSLTIFTFLGLWNDYQTPLI
                     YLNSSENMTLPLAMSYFSTQHLSDLSATMAAASLIMIPASIVFIIFQKQFIKGITMTG
                     MK"
     gene            complement(13594..14469)
                     /locus_tag="ACZPD4_01415"
     CDS             complement(13594..14469)
                     /locus_tag="ACZPD4_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1377771.1"
                     /translation="MKNKKFVGTVMVPAMLHMFVFIVIPIVIGLVISFFNYNPLSSDN
                     KFIGLENFVRLVQDADFRKALLNTLLFVLITVTLNIGIALVVAQMISWFKSNKVRSFF
                     RMVFFLPCIAPMVATSVVFARSIFPTTTGFLNVALNKIGIDSINWLGDPKVVMISLII
                     YTIWVDVGYNVILFSAGIDGIPSYVYEAADLDGASEWVKFRKITWPLLGRSFQFVIVQ
                     TLISHFQMFAQFAVLILKDGPQNSGLVLSRYIYKMAFEYKDMGYASAVSLVLFVIIMT
                     LSLIEQKKSAVDWEY"
     gene            complement(14520..15803)
                     /locus_tag="ACZPD4_01420"
     CDS             complement(14520..15803)
                     /locus_tag="ACZPD4_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1377772.1"
                     /translation="MKKRIVSLILCGLMVFSAAGCSGGGENKAGKDDVTLTMWTHINE
                     PWNAAYKDAIKEFESENEGVRIKLETFPYDEFESKVQTALIDGEGGADIYELWGGWAL
                     DFTPYGTLMELPEEMAEKVREENYEPTYGALEYEDKMYGIPLEYNIEYGGMLVNDTLL
                     KAKGLTVPKTWEELRKTAIEGTEHDGNIFQCKGFDFVNWDSVPYYFLAFILQQDADYM
                     TADGKFDVTTDAAKTAFNELATMVTEDKVTDLEGLTGGADMEGYQQLYAGRNMIVPRG
                     PWTISEGEQSFGLKLGTDFDYAAMPQYGNNQKFASENGWSLAVSSSCKEQEIALKFLE
                     YLYQDDVMLEHNIRCTQIPAKKSLAQSEEYKKEVPYADVLVGILDQGQFIGNFNTDRL
                     KETINNTFVSYCSGEYGSLDEAMEDMNTKLNGILE"
     gene            complement(15784..>16487)
                     /locus_tag="ACZPD4_01425"
     CDS             complement(15784..>16487)
                     /locus_tag="ACZPD4_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR1377773.1"
                     /translation="IEREEVAAEEPVRIEMFKKLGYFNTESSGHNSEYVAWFRKRPDL
                     IEKYCTHGTGWNPGAYGFILDEYLGREDTWEKEYTDWLENGEVDLERGEEYASNIFNA
                     VFGDHTPYFFNGNLKNHGYITNVKQGVCVEVPTVATEAGIVPIKQITLPDHLAVLVNN
                     SALIEDLAVKAAIEGDPNLVFQAVLYDPLTSAVCSMDEILNMVQEMLDKNAEYLSYFK
                     SLKINMEVLHEKENR"
BASE COUNT         4210 a         4517 c         2799 g         4961 t
ORIGIN      
        1 tttcaaggtt caaatatatc tgaaaagcat ttctgcaatt tactcaaaaa atcttcattt
       61 taaccttgac ttttaatagt tagacttttt cctgtttttt tccgaactta aaatctgcat
      121 ttagttcgtc tatactcatc catatctctc agcttatcct atgcatgcat ttcttatccc
      181 gcacatgcat ttatacataa ccgtacacac ctctgaccga gacttctccg gcatagacat
      241 gttccgaagt tccgtcttcc cttctcacga tcaactctcc gtttttatcg atccccagcg
      301 cctttgcctt atatgttccg cttggatcaa gcacaagaac atcccgatcc cggttgacaa
      361 gcatcgacat atacgtttcc atcagtccgg ataaatcttc cgtctcaaga aacaggcggt
      421 aatcttcctc aaaacatttc atgatcgacg caatgacaga cgctcgtttc accatatgtc
      481 cgcattcaag atacagcgat gttgcatgat cttttaattc ttccgggaac ttttttgtgt
      541 ttgcattaat tccgatcccg acggtcacat aattgatata atcgatctgc gtactcatct
      601 ccgtgagaat acctgccagt tttttcccgt tgacaacgag atcgttcggc cacttaatct
      661 gaatatccgg acaggaagct ccctcatttt caaaatattt ttttattcct ttcgccgtac
      721 tgcacgccgt cacgatcgtc agcatagacg ctttatccgg cgtgatcttc ggtcttaaaa
      781 gaaccgacat aaagatcccc gctccggcag gtgattccca cgaacgtcct ctgcgccctc
      841 ttcccgccgt ctgctcttcc gccacagcca gcgttccggc taaagcgcct tcatctccca
      901 tctgcttgat gcgcagattc gttgaatccg tcactttata acatacgaca tttcttccca
      961 cccattccgt atgcatacag ctttccactt cttcttttgt caaaatgtcg ggactttcca
     1021 ccatgcgata ccctttgttt cgcacagctt ccacctcgta gccgtcttcc tgaagccggc
     1081 ggatcacctt ccagaccgca gtgcgcgaca cattgaactt ctcacagatt tcctgccccg
     1141 aaatgtaccc gtctgcttcc cgcagcattt tcaaaatctc tgctttcata attttatctg
     1201 cttttcgcct tctctgattt tactttcttg gctttttgcc ttctcaagtt ttcgtctttt
     1261 tattctctcg actttttatc ttttttacgt tctcagcttt tcgttgtctt tatctttctt
     1321 gctttttgcc tttgtatctt ctctgttttt atcttctttt ctacttattt tttacttcaa
     1381 tgtagcatcc ataaccgctt taatcgtatg catccggttt tccgcctcat cgaacacttt
     1441 cgactgttca gactcaaata cctcgtccgt cacttccata tctgtgattc cgaaacgctc
     1501 gccgatctct cttccgactt tcgttccaag atcatgaaac gccggaaggc agtgaagaaa
     1561 gattgcttcc ggttttgcat tttccatcac ttcttttgtt actttgtacg gagtcagctc
     1621 tctgatccgt ttctcccaca cttcgtcagg ttctcccata gatacccata cgtctgtata
     1681 gatgacgtca gcgccttttg ttccttcttt cacatcgctt gtcaatgtaa tttcagaacc
     1741 tgattctttt gcatatacgc ggcactcctc tacaagctcc tcattcggga aatagtcttc
     1801 tgtcgtacac gccacaaaat caagtccgag ttttgcacag gcgatcatga gcgaattgcc
     1861 catattataa cgggcatctc ccatatatac aagtttcaga cctttcagtt tcccgaaatg
     1921 ttcacggatc gtcagcatat ctgccagcat ctgtgtcgga tgatactcgt ttgtcaatcc
     1981 gttccataca ggtactcccg catactgagc cagctcttcg acaatctcct gtccgaatcc
     2041 tctgtactcg attccttcat acattcttcc aagtactctt gccgtatctt tgatgctctc
     2101 tttcttcccg aactgagatc ccatcgttcc gatataagtc gtccccattc cgagatcatg
     2161 tgccgctatc tcaaaagaac tgcgtgtacg cgtactgtct ttttcaaaga tcagcgccac
     2221 atttttccct ctgtgatgat cgacaagtat cccttttttc ttcttttctt ttaattctgc
     2281 ggcaaggtcc aaaagataaa tgatctcttc cggtgtaaaa tctttcagtg tcaaaaaatg
     2341 tcttcctttt aaatccattg tcttatcctc tcttttcatt tattttttat ctgtatttat
     2401 ttactttcaa ttattcacat ttgttttcat ttactttgat catcatttac agactcgcac
     2461 cgccttattt tctcttgttc cgcctttgtc tgcccgcttc aaacataagc actgccgaag
     2521 ccaccgctgc attcagcgac tctgcgccgc cttccatcgg gatcagtatt ttccgatccg
     2581 cacatgccgc agtttcttct gtcagaccgt ttccttcatt tccgataaga aatgcgcatg
     2641 ccgtcttgta atcttccata tcataggagt tttccccttt aagatgcgcc gcataaaccg
     2701 tgattccttc ccgtttcagt tcgtcgatcg ttcctttcag atcatccgta taaataaacg
     2761 gaatccgaaa aatcgctccc atcgtagagc ggatcacttt cggattatac agatctacgc
     2821 agtctttgct caaaacaatg cctgtagccc ccgccgcctc tgcagtcctg aaaatcgttc
     2881 cgagattgcc cggatcctga atattttcaa gaacgagcac atgcggagtc tcccctaaaa
     2941 tatcttccgc cgtatactcc atccggcgta cgacggcaag gattccctgc ggagtcttcg
     3001 tgtccgacac atgcgcaaat accgtatccg acatcacttc cgcctcttga ataaaccgat
     3061 cgtttttttc tttgacgcat ttcgcctgaa aactttccga cacatacagt tttacaatgc
     3121 gctcccgtgg gatctcttcc gccatgcgcg gtccttctac gataaatacg ccctctttct
     3181 cccgctcacg gctttttttc tgcaaacgaa gcaattcttt tacttgtata ttcgatgtac
     3241 ttgtaatcat tttctcacct gcattttcta tctgtttttc atgtcaaatt tctcaagaat
     3301 atcgttagaa ccgatcacga tcaatatttc tttttcccga agcggctcct gcggattgat
     3361 cgcgacttcg atctttccgt tcttgataat accgacgaca ttgattccca gacgctctct
     3421 gactccgaga tcgacaagac tctttcctac ccagttttgc ggcacgacga tctctaccat
     3481 actgtaatca tttgaaagag agatccagtc cacaaattct ttcgacacga ggctttttgc
     3541 cacgcggctt cccatctcga tctcaggata cacgatacga tccgctccga ctctttgcag
     3601 gatcgcgccc tgaagtttgt ctttcgcctt tgcaacgacg agaggaattc ccatttcttt
     3661 gcaaagcatc gtcgccataa tacttgcctc taaactttcc gaaatggcaa ctaccactcc
     3721 gtcaagatta cgtcctccga gagcctgcag cgcgtcaggc tctgacacgt ccgctttgac
     3781 cgcgtaagaa actttgtcgg atatttcctg tactttctcg taagactcgt ctacgacgag
     3841 cacatcgcat cccatatttt ccaatgtaag cgcaacgctt gacccgaaac ttccaagtcc
     3901 caacaccgca aattgtttat ccataatatg ttccctcctt ttaaatgctt ctgtctttac
     3961 atttccggtt ttgtatttct acttttttat ttctctgtcc atattctcat cttcatgctc
     4021 tgcattgcat tttctttcct aattatccaa gcattacgtt gtctctccca cttagccgag
     4081 catcacattt tcttccggaa gttctctgaa cttagaaatc ttatcgcttc ttcccgataa
     4141 tgcaagcgcc atagtaagcg gtcctatcct tccgacatac ataaggatca taaggatcac
     4201 atgacttccc ctgctcagga tcggagtaag gtctgccgtc agtccgaccg ttcccattgc
     4261 ggatgccgct tcataagtaa gatcaaggaa atcaattccg ggttcaaaga ttgtcagtgc
     4321 ggtaattccc gacatcagga agatcaaagt gatcattgtg atcgtaattc ccgatctgac
     4381 gatattatta tcaatctttc ttgcaaaaca ctctgtatct cttttgtttt tcagtacaca
     4441 gagaaccgtc agcaaaagca tcgcgatcgt cgttgtctta acgccgcctg ccgtacccgc
     4501 cggagatcct ccgatgaaca taagcataca gcccaaaagc ttcgagcttt ctcttaagtc
     4561 tccttgcgga actgtcgcaa atcccgccgt tcttgtcgtc accgactgaa ataaagatgc
     4621 cgtcatcttc tcaagaagat tcatatctcc cattgtttcg ggattgttaa actccagcag
     4681 gaaaaatccg acggcgccgc acaggattag aagtcccgtc atcgtaagaa cgatcttact
     4741 ttgcagacaa agtcttgtaa acagccattt taaaggtctt ttcttgattc ccgtcatctt
     4801 ttttatattg atcgcaatgt catgccatac cggaaaacca agtccgccca taacgatcag
     4861 aaacattgtc gtaaaattaa taagccacga ccctgaatac tgtataaagc tcgtacttcc
     4921 aagtatatcg atacccgcgt tgcaaaatgc cgaaacagaa tgaaagattg cgtaaccgac
     4981 gccttttata aacccaaact caggaataaa ccggagcgaa aaaagtaccg cgccgatccc
     5041 ctctaccaaa aacgttcctt ttaaaatacg gatcacgaac tttacaagac ctgaaagcgt
     5101 atccagtcca tatgcctgtt ggatcgtgat tcttcctttc atattgatct tttttcccaa
     5161 taaaaggaaa aacgcagaca tgcaggcgat cactccaagt ccgccgacct gtatcaaaag
     5221 cagtaaaata attttcccta gcaatgtaaa ctgttccgcc ggaacaatcg tcacaaggcc
     5281 ggtaacgcat acgctgctga cagacgtaaa aagcgcgtct ataaactcga tcggcttctg
     5341 attacagatc ggaaggctca aaagtattcc gcccccgagt atcaccccga aaaatccgat
     5401 aacaaccgtc tgcaccgtat tgaacttcag tctcctcgga ctcttttttt tcttttccat
     5461 ttctaaaaac caccctatga tcttaatctg ttttccccat acacggcata tatattccat
     5521 attatgccgc ttcccttttg acttctatat gatacacgca tttattctca tttacaatag
     5581 aaaaggagtt ccgaaaaacg aaaccccttt tctctcatga caaatataaa atcaaactgt
     5641 gttttcgtca tgccattaca ctttaaagcg atatcccact ccccatatcg tctcgatata
     5701 ctgtggatga gacgtgtcat attcgatttt ctcccgaatc ttcttaatat gcactgtcac
     5761 agtcgcgata tctcctatcg actccatatc ccagatctcg cgaaacagct cttctttcgt
     5821 atacacatga ttcggatgac tcgccaaaaa tgtaagaaga tcgaactctt tcgtcgtaaa
     5881 tgaccgctct tctcctccga cccatacgcg tctcgccgtc gtatcgatct tcaatccgcg
     5941 gatctcgatc acttcatttt cctccgcatt gctgttgatc agccgttcat atctggcaag
     6001 atgcgctttc acgcgggcca caagctcact cgggctaaac ggcttcgtca tataatcatc
     6061 cgctccaagg cccagacctc tgattttatc aatatcgtct tttttggcag aaaccataat
     6121 aatcggcgta ttcttctgat ctctgatctg acggcatatt tcaaaaccgt caactcccgg
     6181 gagcatcaga tcaagaataa tcaggtcaaa atctccgccc agtccttttt gcagacctgt
     6241 atctccgtca ttcgccactt ccacctcaaa gttgctcagt tcaagataat ctttctcaag
     6301 atccgcaata ctctcttcat cctctacgat caaaatacgc ttactcatca atagatacct
     6361 cctgatattt tctgattaca aaatacattg ttgttccggc gccctcttca ctggtcgccc
     6421 atatatttcc tccatgttct tccacgatct tcttcacgat cgacaaaccg attccgcttc
     6481 cgcctttcga tgaattgcgg gaagcatccg tcctgtaaaa gcgatcgaaa atgtacggca
     6541 ggtcttttgc ggcgatccct ttgccgttgt cgcctaattc aacctgtata aaatcgccga
     6601 catcttttac ttccatcgtg acttttccgt tcggcttgtc catatatttc aacgaattcg
     6661 atacgatatt attgatcacc cttctaagct gctcgggatc aacgatcacc ttgcagtctt
     6721 cttccatcgt attgcaataa gtaaattcga ttcctttcga ctcaagctcc atatgcagat
     6781 cttccccgca gtctccaaaa taccctttcg ccgatatcgt cgtaaaatta tacggaatac
     6841 gattcgtgtc gatcttggaa taaagcgtca gttcgttgat taaaagatcc atctcatttg
     6901 ctttattata tattgtctta atgtatctgt ccatcctttc gggggtatcc gcaaccccgt
     6961 ccatgattcc ttccacatat cctttgatcg ccgtgatcgg tgttttcaga tcgtgggaaa
     7021 tattactgat cagctccttg ttctcccggt cgaatgcaac cttctcttcc gcgttcgcct
     7081 tcagcctaag cctcatctcc tcaaaatcct gacagagttg tccgatctca tcatccgact
     7141 cctgcctgat ctcaaaatca agattccctt ccttgatatt tcttgccgcc ttctgcagtt
     7201 tcgccagcgg aacagaaacc gaacggtaga tccaataaat cagcagcgtc gttgtgatga
     7261 atacgacaat catataaagc tgctgaagtt ctcctacttc ccgcgacgcg aacgtatgca
     7321 tcactacatt caaaaagatc atcggcaaaa tcataacaga cataaatgcc acgatcaatt
     7381 ttgtcctaag cttcataggc gctcctccta aaacagattc tcacttttgc atgatattat
     7441 atattatacc gttacccctc cccttcttca acgaaacttt ttataaacaa atcagccgcc
     7501 tcttcgatat ggatatccgt taccctgtat cgaaaagacg gccgaatcgt catcttagtt
     7561 ttctatcctt tttctatgcc ggtctttccc gccggtaaat ctgttttatg attcaagata
     7621 ttttttcaga tcgtctgctt tgtccagttt ttcccacggc agttcaacgt caagtcttcc
     7681 aaaatgtccg tacgcagcgg tctgcttata gatcggtctg cgcagatcca gcattttaat
     7741 aattcccgcc ggacggaaat caaagttctc acggatgatc tccacaagct ttgtatccga
     7801 aaccttccct gtgccgaacg tatctacgga taccgacgtc ggctgcgcta cgccgatggc
     7861 ataagaaagc tggatctcgc atcgtctcgc aagacctgcc gcaacaacat ttttcgccgc
     7921 ataacgcgcc gcatatgccg cggaacgatc cacttttgta caatcttttc ccgagaaagc
     7981 tccgccgccg tgacgcgcca ttcctccgta agtatcaaca atgattttac gtcctgtcaa
     8041 accgctgtct ccatgaggtc ctccgatcac aaaacgtcct gtcggattaa tgaaaaactt
     8101 tgtttcatca tctgtcatat ttgccggaat cacttcatcg aatacatatt tcttaatatc
     8161 ttcatgaatc cgctcctgcg tcacttcagg atcgtgctgt gtagaaagca caaccgcatc
     8221 caaacgcttc ggaacgccgt tttcatcata ttccacagtt acctgtgttt tcccgtccgg
     8281 gcgaagatac ggcagtgttc cgttctttct tacttctgtc agacggcgcg caagcttatg
     8341 cgccatcgcc gccggatacg gcatatattc ttctgtttca tcagacgcat aaccgaacat
     8401 cattccctga tctcctgctc cgatggcttc aatatcgtct tctgacattt tattttcttt
     8461 tgcttcgagc gctttatcca ctcccatcgc aatatcggac gactgttcgt caatcgttgt
     8521 gatcacgccg catgtatcgg cgtcaaatcc gtattttcct cttgtatagc cgatttcacg
     8581 aaccgtgtca cgtacgatct tcggaatatc tacataagct ttcgtcgtga tctctcccat
     8641 caccataacg acgcctgtcg tacagcatgt ctcacatgcg acacggctca tcggatcctg
     8701 cttcataagt tcatccaaaa tagcgtccga aatctgatcg cacatcttgt ccgggtgtcc
     8761 ttccgtaacc gactcggaag taaataaaat cttttctctc tccataacca ttctcctttc
     8821 gttcactttc tctatttctt tacgatcaag aaagatatct gcctgcggca gactgcttcg
     8881 gatcataagc agtcgtcaag atccccggat gacccggact ttgactcgcc gccgcaaata
     8941 aaaacagccc gtaaaagcgg actgtctctc tatcacaaca atcctcttat tgttcgatga
     9001 ctcgctcagg ttggcacctt cctgttcagg ggttgccgca gcttctcaga tcctttgtat
     9061 ctccactgct ctgaataaga gaaagtattt ttattaaact gttttgttac aaacaagact
     9121 ttacaatgta cactcggaaa tgtcaatgct tttcttcatt ttcctgcaga agatattttt
     9181 catcttcccc tttaccggtc aggttctcaa gccacactcc ctttttcacc attaccgcgc
     9241 ccgcaataca cttcagccat tccccgatct gcatcgcact gtagaccgcc cacggggcaa
     9301 gtccggtata tcggcataaa acaaaaacga tcggaatgtt gacgcaccat aagaatacac
     9361 agtcaaagaa aaaagtgaca cctgttttcc cgcctgcccg aagggtaaaa taagcggcgt
     9421 tgagaaatgc attctgggga ataaacaccg cctgtgccaa aacaagtccc atagccgcct
     9481 ttcgcacagc ttccgtcgta ttataaatcc gggggaaaaa cggcgcgaaa agaaacataa
     9541 aaaccgatac tgcgaaactg ataagaacgc aggatacaat catcttattg tcgatctctc
     9601 tcgctctttt catatctccg aagccaagtg cctgcccgac tatgatcgcc acggattctc
     9661 ccatcgccgc aaacacaacg ctgaataagt tattgatcgt attcgctatg ttcatcgccg
     9721 cgatcaccgt cagcccgcgc acagaatagt tttgtgccaa aaccgccata ccgaaagacc
     9781 ataacgcttc gttcagcaaa agcggcgttc cctttataaa atattttttt acatgatccc
     9841 ggggcacgga caacgtttta taaagtcctg tcatatatgt attttctttc tcatgggtat
     9901 gcgcccatat tacgacgatc gccgtttccg tataacgcga aagcaccgtt gccacagccg
     9961 ctcccgccac ccccagccgg gggaatccga actttccgta tatcagcaga taattgaaaa
    10021 taagattgac cgccacagcc gcagcccccg ccttcatcgg cactactgtt tccccgcact
    10081 cacgcatcgt actggcataa acctgcagga tcataaacgc cggaagatcc aataagataa
    10141 tccgaagata cgtcatccca tattccagcg ccagcttttc atttcctccg ttttctcccg
    10201 tcagatacaa aagaatcagc tgctttccga aaaacagaag aagaaaagcg gcgccgacag
    10261 taattatcag cgccaaccaa atcttatatc ggaatgtact gcgtatccct tcatcatttt
    10321 tttgtccgaa atattgggcg gtaaagattc ccacacctgc tgttccgccg aaaatacata
    10381 aattatacac aaatatgatc tgattgacga tcgaaacacc cgacatctct tctgttccca
    10441 gtcttccaac cataatattg tccaaaaggt tcacaaaatt tgtaagtccg ttctggatca
    10501 tgatcggaac ggcgatcgcc atgaccatca tataaaaggc tttatttcca atatattttt
    10561 tatacttttc tttcattttt tcatatcaat tttatacccg catatgctgt gccgtacgca
    10621 gattttctat catctgttca ctcacttcgt ttcccgcagc tctacacgcc agtataatcc
    10681 cgcctgccga cgggagcaga cacggctctg tgaaaacgca cgatacgtcc cgcagctctt
    10741 cttccagcgt ttcttttata tacttcatcg ctttgaacac acctccggaa taagacacat
    10801 aaaccggtat atcaaaattc ccgtttctgt acacgccttt tacgatcttc gcaagttcgc
    10861 gcgccgcgct tttaaaaatg cctgccgccg cagaatcgcc ctgttccgca agttcatatg
    10921 catcttttga catagatgcg atcttatccc gttctccctt ccactcatta ataacgagcc
    10981 ttaatatctc ttcaggacac gcaagaccgt atttttccaa aatataatca aacatcatcg
    11041 ttttttcttc tcttccgtcc gcctgcattg taaaatgccg gatcaactga catccgatcc
    11101 aataaccgct tccttcatcc cctccgaaaa gatgatgcca tccgccgctt cgcacatagt
    11161 aactgtcctt gtcaaatcca agcccgatcg atcccgttcc ggcgatcaca tggattccct
    11221 gttttccgag aagagatcct gccagtgcat tttccgtatc gttcccgaga gtgatcgaca
    11281 tcctcggaaa cgtttcccgt acaaggcggg taagcaccgg cacatccgcc ggtatatctt
    11341 taaaccctgc gatagctata aaactgtgag tgatctcact ttgcgcgatt gccgcctgac
    11401 agcagatctg caaaataccg tcttttaata ctgacagcac atgatcgatt ccgttcacca
    11461 tatagttgcc ggggcctaac gtacacgcag cttccagctc tcccgtttca cggcacaaaa
    11521 gaaacgccgt ctttgttccg ccacaatcaa cgcttaaata ccgtctcatc tcacacctcc
    11581 tacaaagcgg ttttcatcaa gagaaagaac tttacatgca tttttataat atatcttttt
    11641 caagatctca tcttccagtc cgattccgta gattgcccat cttccctgtc ccggaagttc
    11701 tccttcgggc tgatacggaa aatactcgtc ttttgtctcg aagaaacgat aataaatctt
    11761 atgatatccc ataccaagcg gcgtacaatc cgtaccgaac aagatccgat cctgatgctt
    11821 cacaaaaaac ttcttcgcac tgtacggaac tcttcccagc tccgccagcc gggccgcaac
    11881 atctatatac atattcggat atttttccag ctgcccgctt acccattcca gattctccgc
    11941 gtaacttccg acatgagcga tcacaaacgt cgtttccgga tgactgcgta ccgtattctc
    12001 ttgcatttcc ataagttctt caaaactcat atacttttca tgatctgaaa aatcccattc
    12061 cggacagaca tcaagctctt cccatcgttc atttttttca cttttcggag taaaaaacgc
    12121 cgccggatca gctacatgca tcagcaccgg aatgtgcagt ttcgccgccg tatcatagat
    12181 gatatcaaaa cgaggatcat ctgtacggat cggcctgccg tatttatccc gctcccacag
    12241 cgaaagatct ttccataatt tgatcccccg gcatccgcgc atacaggaat cttctattac
    12301 ctttttcgtt ttatcgcaaa aatcaggatc atcatacgca gaaaaatcaa gctgcataaa
    12361 attaaaaaac atttcctcga atccttcttg tttcttttgc atcttttcca gatcttttcc
    12421 gaaatatcca tccatattca cagcgcatac aacgccggca tcttgcagtt cccggacata
    12481 ttccttcgtg tcatactttg tttcatacga ttcgcccaac agcatttttc ccatatgcat
    12541 atggaaatct atcaccggat atgccggttt ttcaatttct gtttttttca gcactgcggc
    12601 gcagacagga tcaaattttt ccaattccat acagaatcac cccttctgat tctctttttt
    12661 atgttttcct ctttttatgt tcttagcatt tacattttat ctttatactt cttgcttttt
    12721 taactttttc ttttccacat ttttcgttat ttcattccgg tcatcgtaat tcctttgatg
    12781 aactgcttct ggaatataat aaatacgatc gacgcaggaa tcataataag ggaagcggca
    12841 gccatcgtcg ccgaaagatc cgataaatgc tgagttgaaa aatacgacat tgcaagaggc
    12901 agagtcatat tttccgagct gttcaaatag atcaacggcg tctggtaatc attccacaat
    12961 cctaaaaagg taaagatcgt cagacttccg agcgccgggc ggattcccgg aagcacgatc
    13021 tttcgataga tcgtaaacgg ccccgcatta tcaagcatcg cacactcgaa aagttccttc
    13081 ggcatatcgt cgataaactg tttgcacagg aaaattccaa atgcatttac aaacgtaggc
    13141 acaacaagag aaatgatctt atcataccat cccagttttg atataagcaa aaagttcggg
    13201 atcatcattg ccgccgtcgg catcatcatt gtgatcaata ttacataaaa caaaaatttg
    13261 ttcagtttga atttatattt cgacagcaca aatccgatca aagagctcgt aaacaaaacg
    13321 acaagcgtat caataaccgt tacaaaaagc gaatttccca tccatctgaa aaacggcgaa
    13381 tctgtaatca ctttcacata tccgtcaagc gtaaactgct tcggcagcat attcgtaggc
    13441 tccagcaaaa tctctttcgt agatttaaag gaattggaaa gcatccaaaa atacggcgca
    13501 agaaacacga tcgctgccaa aaacaaaatc acgaatgaaa taatccttag ttttctgttc
    13561 tttttgtctt ttaacatttt cataatatgc ctcctaatac tcccagtcta ccgcactctt
    13621 cttctgctcg atcaatgaca gtgtcataat aatgacaaag agaacaagcg acacagccga
    13681 tgcatatccc atgtctttat actcaaacgc catcttgtaa atatatcttg aaagaacaag
    13741 tcctgaattc tgagggccgt ctttcaaaat gagcactgca aactgcgcaa acatctgaaa
    13801 atgtgagatc aacgtctgta caatgacaaa ctgaaagctt cttccaagca aaggccatgt
    13861 aatcttgcgg aattttaccc attcgctcgc tccgtccaga tctgccgcct catacacata
    13921 agaaggaatt ccgtcaatac ctgccgaaaa caggatcacg ttataaccga cgtctaccca
    13981 gatcgtataa ataataagtg agatcatcac tactttcgga tctccgagcc agttgatcga
    14041 gtcgatcccg atcttattca gagccacgtt cagaaatcct gttgtcgtcg ggaagataga
    14101 acgggcaaat acaacggatg ttgcaaccat aggcgcaata cacggcagga aaaataccat
    14161 acggaaaaaa cttctcacct tatttgattt aaaccatgag atcatctgcg ccacaacgag
    14221 cgcgatgccg atattcaaag taaccgtgat gagcacgaat aacagcgtat ttaaaagcgc
    14281 cttacgaaaa tccgcgtctt gaacaagcct tacaaagttt tccagtccta taaatttatt
    14341 gtctgaactg agtggattat agttaaagaa agaaatcaca agacctatca cgatcgggat
    14401 aacgataaat acaaacatat gcagcatagc cggtaccatg accgtcccta caaatttttt
    14461 atttttcatg acgcaccccc ttgtgagtca gactgccgtt ttacggcagt ctttttattt
    14521 tattccagta ttccattcag tttcgtattc atatcttcca tcgcctcgtc caacgatccg
    14581 tattccccgg aacaatagga aacaaacgta ttgttgattg tttctttcag acgatccgtg
    14641 ttaaagtttc cgataaactg tccctgatcg agaattccta caagaacgtc cgcgtacgga
    14701 acttcttttt tgtactcttc gctctgagcc agacttttct ttgccggaat ctgtgtgcag
    14761 cgtatgttat gctctaacat aacatcatcc tgatacagat attcaaggaa tttcaaagcg
    14821 atttcctgtt ctttacaact ggaacttacc gccagcgacc atccgttttc cgaagcaaat
    14881 ttctgattgt ttccatactg cggcatagct gcataatcaa aatccgtgcc gagttttaat
    14941 ccgaatgact gttctccttc ggagatcgtc cacggtccgc gcggtacgat catgttgcgt
    15001 ccggcatata actgctgata accttccata tccgctccgc ctgtcagtcc ttcaagatct
    15061 gtaactttat cttctgtcac cattgtcgca agttcattaa acgctgtctt agccgcatct
    15121 gtcgtcacgt cgaattttcc gtccgccgtc atgtaatccg catcctgctg aaggataaag
    15181 gcaaggaaat aatatggcac ggaatcccag ttcacaaaat caaaaccttt gcactggaaa
    15241 atattgccgt cgtgctctgt tccttcgatt gccgttttac gaagttcttc ccatgttttc
    15301 ggaacagtaa gccccttcgc cttgagcaat gtatcgttca caagcattcc gccgtactcg
    15361 atattatatt ccagcgggat accatacatt ttatcctcat actcaagtgc tccgtatgtc
    15421 ggctcgtaat tttcttctct cactttttct gccatttcct ccggcagttc catcaatgtt
    15481 ccgtaaggag taaaatctaa tgcccagcct ccccacaatt cataaatatc cgctccgcct
    15541 tcaccatcga tcaaagcggt ctgtactttt gattcaaact catcatatgg gaacgtctca
    15601 agtttaatcc gaacgccttc attttccgat tcaaactctt tgatcgcatc tttatatgcc
    15661 gcattccacg gctcattgat atgtgtccac attgtcagtg taacgtcgtc ttttcctgct
    15721 ttattttctc cgccgcctga acatcctgcc gctgaaaata ccatgagccc acataatatt
    15781 aaactaacga ttctcttttt catgaagcac ctccatattt atctttaacg acttaaagta
    15841 actcaaatac tctgcatttt tatcaagcat ctcctgaacc atgttcagga tttcatccat
    15901 gctgcagacc gcgcttgtaa gcggatcata caaaacagcc tggaatacaa ggttcggatc
    15961 gccttcaatg gcagctttca cggcaaggtc ttcgatcagc gccgaattat ttacaagcac
    16021 tgccagatga tccggaagcg tgatctgttt gatcggaacg attcccgctt ctgttgcgac
    16081 tgtcggaact tccacacata cgccttgttt cacatttgta atataaccat ggtttttcag
    16141 gtttccgtta aagaagtaag gtgtgtgatc gccgaacact gcgttgaaaa tattggaagc
    16201 gtattcctct cctctttcaa gatcgacttc tccgttttca agccagtccg tatattcctt
    16261 ttcccatgta tcttcccgtc ctaaatactc atccagtata aatccgtacg ctcccggatt
    16321 ccatcctgtt ccgtgagtac aatatttttc gatcagatcg ggacgttttc taaaccatgc
    16381 cacatattcc gaattatgtc ccgacgattc tgtattaaaa tatccaagtt tcttgaacat
    16441 ttcgattctg acaggttctt cagccgccac ttcttctctt tcgattg
//
LOCUS       DBMDXF010000017        16245 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1075_length_16245_cov_2.446853, whole genome shotgun sequence.
ACCESSION   DBMDXF010000017 DBMDXF010000000
VERSION     DBMDXF010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 16245)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16245)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16245
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1075_length_16245_cov_2.446853"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="ACZPD4_01430"
     CDS             <1..648
                     /locus_tag="ACZPD4_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1377774.1"
                     /translation="KDLNKIITARLKDEYYSVDSCFDGKEAEEYLTAVKYDAVILDIM
                     MPKKDGLSVLKEMRRKKDSTPVILLTAKDSVEDRVAGLDAGANDYLVKPFAFEELLAR
                     VRVLLRKPEEMPKTCYTIADLEVHLDTHKVIRGKEEIRLSGKEFALLRYMVQNAGIVL
                     SRDRLEQHLWNYDYMGSSNVIDVYIRYLRKKIDDGREKKLIHTVRGVGYVLKEEE"
     gene            645..2051
                     /locus_tag="ACZPD4_01435"
     CDS             645..2051
                     /locus_tag="ACZPD4_01435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1377775.1"
                     /translation="MKKVSLKIKLTLLYTIFILVVVGTVLGILFSLSGREILASTKMS
                     LERRVEESLEEIEMQDGELKIDSDFYSVENGVYLSMYDSTGYFLYGKIPGGFDRQPDF
                     LDGEVREIKDKAGEEDWYIYDLFFRPGEGKEIYVRGVISVTESEESFQTILRIAFILL
                     PLLAAATAFVGYRFTKRTLKPVKDITDTVCKIRMDADLSRRIDNIDTVRETKEKRYKK
                     NRDEIYILAETFNEMLDELEAVFKREKQFTSDVSHELRTPISVMMAQCGQLLKDEALK
                     PKQKAQVKLIEQKAENMSDLIAQLLFLSRADQGRQQIEKENVNLSELTEMIVGEQQFL
                     AESKGNAGQIICHIEPEIWAEVDETLYIRMMINLLSNALRYGEGRDIEVSLSVHNDSD
                     IIGKVKDHGIGISKGDIPHIWERFYRADKSRTGGSHCGLGLSMVKWIAEAHGGSVEVV
                     SKEGEGSEFVFHLRKSKS"
     gene            2212..2823
                     /locus_tag="ACZPD4_01440"
     CDS             2212..2823
                     /locus_tag="ACZPD4_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR1377776.1"
                     /translation="MKKFTKIATVAVMTMVTAGVFAGCGLKGGKDIGKEAALEAALAD
                     AGVRESDTTRLRVSSEIDDGRKVYEVRFDVEGTEYDYEISGRDGQILSADTERISTVD
                     TTDRAGTTDTAGTADTAGTTDKEKSGNSQNNAEALGISIEEAEKIALERVPGASENDI
                     RIQSDYDDGKYKYEGDIIYDGKEYEFEIDASTGTILEWSEERY"
     gene            2958..3623
                     /locus_tag="ACZPD4_01445"
     CDS             2958..3623
                     /locus_tag="ACZPD4_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HIR1377777.1"
                     /translation="MNYLWAGMILIGIIFGAFNGRMEVITNAALDSSKEAVTLCITMI
                     GIMSFWCGIMEIAAQAGIIGSAAKKMRPVIRFLFPDLPKEHKAQEHITTNFIANILGL
                     GWAATPAGLKAMKELETLEEERRAGKVPGPVRRKGVASNEMCTFLIMNISSLQLIPMN
                     VIAYRSQYGSANPAAIVGAGILATVASTGAAVIFCKIAEQRRKKSGIIKESRYPVKNR
                     ESA"
     gene            3685..4392
                     /locus_tag="ACZPD4_01450"
     CDS             3685..4392
                     /locus_tag="ACZPD4_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR1377778.1"
                     /translation="MDKRYSEEEKDTREELKKEIKREIKKQNRKLKIKIPWKIQKILI
                     IIAIIALAGGAGAIGMKRYITHESQTTKLGFEDIGELATQEANCTEVNVTEDVRKLFG
                     IDIPFTQSKYIYSYNVIVKAGYDFGEIRYEVKKDSIKVKLPEVRILSSQVDEDSFRVY
                     HEKESIFNQIRLEENNAAFAKLKEQAEKDAVANGLYENARSNAKTILKGYFAQAYDLD
                     DYKITFEGGEGGNTNEK"
     gene            complement(4448..5551)
                     /locus_tag="ACZPD4_01455"
     CDS             complement(4448..5551)
                     /locus_tag="ACZPD4_01455"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1377779.1"
                     /translation="MNTKPVFHGSDIEKICEYYHLNQEDIVNFGANVNPIGISTHVKQ
                     VLASHLDLLSSYPDRDYQSLRNTVSEYCGVPADYVLPGNGSSELISLLIETRAPKRTL
                     LLGPTYSEYSRELSLSNSKCNEFLLKETDEFILNVDFLCQSLLAGSYDFLIICNPNNP
                     TSSAILRSDMERLLTFCEQHDIFVMIDETYVEFAPVIEDVTAVPFTKTFKNLMVLRGV
                     SKFFAAPGVRLGYGITGNMPFLKAMKEKQIPWSLNSLGAFAGELMLKDEEYIRQTRRL
                     ILSEREKMYSALKEQPAYKTYKPYANFLLVKLLQKETTAFDVFTHCVKNGLMIRDCSS
                     FKSLDGEFIRFCVMDSKDNQRLLDELSFLCRQK"
     gene            complement(5642..5884)
                     /locus_tag="ACZPD4_01460"
     CDS             complement(5642..5884)
                     /locus_tag="ACZPD4_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1377780.1"
                     /translation="MEYKPKGVCSRLIHFDIVDNKVRNVSFVGGCSGNLQGISHLIEG
                     MDVDEAISRIEGIRCGFKPTSCPDQLAIALKEARNA"
     gene            6339..7289
                     /locus_tag="ACZPD4_01465"
     CDS             6339..7289
                     /locus_tag="ACZPD4_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377781.1"
                     /translation="MLETMMVSSGSVMYVMAAVGILGVLAKIVNRMTLYRLIKAAGNM
                     PKSTHKLMKLVKAKYEHACMAHESVENVGVFVEKYIYEYRGFLFRIHTWRQIELLSVW
                     FVGILAVIGAALSYMEGGLTETVYQYIAGGAAEAVLLMVIIRMSDEPYKIKAIKMYMV
                     DYLENVCAVRVRRQNRKEKEFIDVIPSENVNGGGKVKEQKTFAGADDKKEGASAGTVP
                     VQENRADKKENAKGAATMQETEYEQWRAQAVREILDETGSSMVEKRLPINIEGEPQER
                     KKAEKLSQNVGRGGGEHEPDSGHPQIREETIRQILEEFLA"
     gene            7397..8746
                     /locus_tag="ACZPD4_01470"
     CDS             7397..8746
                     /locus_tag="ACZPD4_01470"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1377782.1"
                     /translation="MMSKVTFDYSKACGFVQDHEMQYMSELAEQAKEKLLARTGAGND
                     FLGWIDLPVDYDKDEFDRIKKAAEKIREDSEVLLVIGIGGSYLGARAAIEFLGHSFAN
                     VVSKEVRKSPEIYYVGNSISSTYVKDLIDVIGDRDFSINMISKSGTTTEPAIAFRVFK
                     ELLEKKYGKEEAAKRIYATTDKAKGALKNLATEEGYETFVVPDDVGGRFSVLTAVGLL
                     PIAVSGADIDKLMEGAASGRKAALEADFEKNGAMQYAAVRNILLRKGKTVEVLANYEP
                     SLHYVSEWWKQLYGESEGKDQKGIFPASVDLTTDLHSMGQFIQDGNRILFETVLNVEK
                     SRAEIVINEEPVDLDGLNYLAGKNVDFINKSAMNGTILAHTDGNVPNLMVKIPEQNEF
                     YLGELFYFFEFACGVSGYLLGVNPFNQPGVESYKKNMFALLGKPGFEKEREELLKRL"
     gene            8902..9978
                     /locus_tag="ACZPD4_01475"
     CDS             8902..9978
                     /locus_tag="ACZPD4_01475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1377783.1"
                     /translation="MLRIGMLTSGGDCQALNAAMRGVVKGICSKRDDVEIYGFKDGYK
                     GLIYADFSMLTPKDFSGILTRGGTILGTSRQPFKLMRVPDKDGLDKVEAMKHTYHKLN
                     LNCLIILGGNGTHKTANLLREEGLNVITLPKTIDNDLWGTEMTFGFQSAVDIATDTID
                     RIHTTATSHSRVFIIEVMGHKVGYVTLHAGIAGGADIILLPEIPYDINVITDVINRRS
                     REGKPFTILAVAEGAISKEDAKLKKKEYKEKISKRKYPSIAYELAEQIQEATDKEVRI
                     TVPGHTQRGGSPCAYDRVFATRVGAKAAEMILEGKYGYMVAMRDGETTAVPLEEVAGK
                     LKMVDPKCGLIKEAKTIGISFGDE"
     gene            10016..11635
                     /gene="dnaX"
                     /locus_tag="ACZPD4_01480"
     CDS             10016..11635
                     /gene="dnaX"
                     /locus_tag="ACZPD4_01480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR1377784.1"
                     /translation="MSYTALYRKFRPAEFGDVKGQDHIITTLQNQIKANRIGHAYLFC
                     GTRGTGKTTVAKIFAKAVNCEHPVDGSPCGECEMCKSIAAGTSMNVIEIDAASNNGVD
                     NIREIREEVTYRPTEGKYKVYIIDEVHMLSIGAFNALLKTLEEPPEYVIFILATTEVH
                     KIPITILSRCQHYDFKRISIETITERMQELMDTEHVEAEDKALRYIAKAADGSMRDAL
                     SLLDQCIAFYLGQKVTYEHVLEVLGAVDTDVFSRLLRKVINTDVPGVLDVVEELVMQG
                     RDLTQLTADFTWYLRNLLLVKTSDNIEDVLDVSTENMMQLKEESGMIETAALFRYIRV
                     FSELSGQLKYATQKRIMLEVALIKLCTPAMETKQDTILERIRAVEEKVEKSMEASANM
                     QGQIVYVNGDGAQIAGGSVINGNTEGDFAGARAQRPQIPDAIPEDVQEVVKNFRMIAG
                     EASGIIKGYLKSARLSLGGDNRLLIVLPDSLAASVVGREDHVEELEKLIEERIGKRVE
                     VEVRHVEEGRHFEDTFIDIEQKINMKITVED"
     gene            11638..11991
                     /locus_tag="ACZPD4_01485"
     CDS             11638..11991
                     /locus_tag="ACZPD4_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR1377785.1"
                     /translation="MAKRGGFPGGAMPGNMANLMKQAQKMQRQMEEQQKELEAKEFTA
                     TAGGGAVEVTVSGKREVTKVKLAEEVVDPDDIEMLEDLIVAATNEALRQIDEATGSAM
                     SKLTGGMGGGIPGMF"
     gene            11994..12590
                     /gene="recR"
                     /locus_tag="ACZPD4_01490"
     CDS             11994..12590
                     /gene="recR"
                     /locus_tag="ACZPD4_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR1377786.1"
                     /translation="MEYYSNQISRLIEEFSRLPGIGSKSAQRLAFHVINMPLDQVEGL
                     AKSIIDARKNVRYCKVCCTLTDRETCPICGNPDRDKKTIMVVETPRDLAAYEKTGKYN
                     GVYHVLHGAISPMLGIGPGDIRLKELMERLQGDVNEVIIATNSSLEGETTAMYISKLI
                     KPAGIKVSRIASGVPVGGDLEYIDEVTLLRALEGRTEL"
     gene            12662..13498
                     /locus_tag="ACZPD4_01495"
     CDS             12662..13498
                     /locus_tag="ACZPD4_01495"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1377787.1"
                     /translation="MDKLKLMKTANEVRKGIVTSVHSAKAGHPGGSLSAADLFTYLYF
                     EELNVDPKDPKKADRDRFVLSKGHTAPGLYAALAEKGFFPKEDLITLRHTGSYLQGHP
                     DMKCIPGVDMSSGSLGQGVSAAVGMAIAAKISGDDYRVYTLLGDGEIQEGQVWEAAML
                     ASHHKLDNLLVIVDNNNLQIDGEITKVNSPYPIDKKFEAFNFHVINIDGNDFDQIDAA
                     FKEAKSVKGRPTVIIAKTVKGKGVSFMENQAGWHGKAPNDEEFKIAMADLEKAGEALC
                     QK"
     gene            13486..14427
                     /locus_tag="ACZPD4_01500"
     CDS             13486..14427
                     /locus_tag="ACZPD4_01500"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1377788.1"
                     /translation="MSEVKKIATRESYGETLVELGAEHEEIVVLDADLAAATKTGLFQ
                     AAYPERFINCGIAESNMIGVAAGLAAAGKVPFASSFAMFAAGRAYEQVRNSVGYPHLN
                     VKIGATHAGISVGEDGATHQCNEDIALMRTIPGMVVINPSDDIEARAAVRAAYEYVGP
                     VYMRFGRLAVPVINDRPDYKFELGKGVVLREGKDVTIIATGLPVNNCLQAAEKLAAEG
                     IDAKVINIHTIKPLDEELIIAAAQETGKVVTVEEHSVIGGLGSAVCDVLSANVPTKVL
                     KIGINDVYGESGPALELIEKYGLGINGIYEKVKAFVS"
     gene            14599..15807
                     /locus_tag="ACZPD4_01505"
     CDS             14599..15807
                     /locus_tag="ACZPD4_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1377789.1"
                     /translation="MERQLPKNVRQIGNVSDSPKIYVEDYVDTFFAGLCEKCEKAGTD
                     PMGAFLVGDIQNGEDEDYVYIYGAIQMHDLKMSDKEYVIDEAAWKNAYEDCKQYFEDG
                     EMLGWFVVHPGAPLIPEPETVKLHKKSFPKKNTVFIMKDPVEKEETYYVHKMNDLLEI
                     GGHYTYYEKNPCMQNYMISSRKKNGASASETVEDRAAKDFRSLARKRSEHQKGRRPGS
                     LMYAAGACVILILVIMGVSLFNSFDRMKSVQNTLGFLDKETVASDGKTSEETTDDGSV
                     PTSAEIEAVKGQTDADSADENKTKEESVSGQKEDGKDEKNENPENSLSESNDKKEPEG
                     SDSAQGADEQTIGSANGQDGVYIVEKGDTLAIISRKIYGDLDHVDTICKMNGITDGNL
                     IYIGQKLLLP"
     gene            15887..>16245
                     /locus_tag="ACZPD4_01510"
     CDS             15887..>16245
                     /locus_tag="ACZPD4_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1377790.1"
                     /translation="MTQKKKTDFKIVTPPDDLSEVRAKTKKIHREKLKKIVVPVILIA
                     LAVSGTYLMLTNKAYSEAGTAVRYSTDSSDTSNYAHFANGIVRYNRDGVVFLNKKNEE
                     KWIQSTQLKNPIIEVKE"
BASE COUNT         5316 a         2606 c         4393 g         3930 t
ORIGIN      
        1 aaggacctga acaaaattat aacagcgcgt ctgaaagatg agtattatag tgtggattca
       61 tgttttgacg ggaaggaagc cgaggaatat ttgactgccg taaaatacga tgcggtaatt
      121 cttgatatta tgatgccgaa aaaagacgga ttatccgttt tgaaggaaat gaggaggaaa
      181 aaggattcta caccggttat attactgact gcaaaagaca gtgtggaaga tcgggtggca
      241 gggcttgacg caggtgcaaa tgattatctt gtaaagccgt ttgcatttga agagctgctt
      301 gcccgggtac gggtgctttt gagaaagccg gaggaaatgc cgaaaacatg ttacacgatt
      361 gcggatctgg aagtacatct tgatacgcat aaagtaataa gaggtaaaga agaaatcaga
      421 ctttcgggaa aagagtttgc gctgctgaga tatatggtcc aaaatgcggg aattgttctt
      481 tcacgagaca gattggagca gcatctgtgg aattatgact atatgggaag ttcaaatgtt
      541 atagatgtgt atatccggta tttgaggaaa aaaattgatg acggaagaga gaaaaaactc
      601 attcatacag taagaggagt cggttatgtg ttaaaggagg aagaatgaaa aaagtttctt
      661 taaagataaa attaactctt ttatatacga tatttattct tgtggttgtc ggaacggttc
      721 ttggaatatt gttttctttg agtggacgtg agatacttgc gtctacgaaa atgtcactgg
      781 aaagaagagt agaggagagt ctggaagaaa ttgaaatgca ggacggagaa ttaaaaattg
      841 attcggattt ttattctgtg gaaaacggag tctatctttc catgtatgac tcaaccggat
      901 attttttata tggaaagatt cccgggggat tcgatcggca gccggacttt ttggatggag
      961 aagtaagaga gattaaagat aaggcaggag aagaagactg gtatatatac gatctttttt
     1021 tcagaccggg agaaggtaag gagatatatg tacgcggagt gatatctgtg acggaatcgg
     1081 aggagagttt tcagacgata cttcgcatcg cgtttattct tcttccgctg cttgccgcag
     1141 cgacagcgtt cgtaggatat cgcttcacaa aaagaacgct taagcctgtg aaagatatta
     1201 cggatactgt gtgtaagata cggatggatg cggatctttc gagaagaatt gacaatatcg
     1261 atacagtaag agaaacaaag gaaaagagat acaaaaaaaa cagagatgag atttatatcc
     1321 ttgcggagac gtttaacgag atgctggatg agctggaagc cgtgtttaaa agagaaaagc
     1381 agttcacttc ggacgtttcg catgaattaa gaacgccgat aagcgttatg atggctcagt
     1441 gcggtcagct tttaaaagat gaagcgttaa agccaaagca aaaagcacag gtgaaactga
     1501 tcgaacagaa agcggaaaat atgtcagatt tgatcgcgca gcttctgttt ttatcaagag
     1561 ccgatcaggg gcgtcagcag atcgagaaag aaaacgttaa tttaagtgaa ctgacggaaa
     1621 tgatcgtcgg tgaacaacag tttcttgctg aatcaaaagg aaacgccggt cagatcatat
     1681 gtcacattga acctgaaata tgggcggaag ttgatgagac gctgtacatt agaatgatga
     1741 taaatcttct gtctaatgcg ctgcgatacg gggaaggacg ggatatagaa gtgagtttat
     1801 ccgtgcataa tgattcggat attatcggaa aagtaaaaga tcatggcatc ggcatttcaa
     1861 agggggatat tcctcatata tgggaacggt tttatcgtgc ggataaatcc agaacaggag
     1921 gaagtcactg cgggctcggt ctgtctatgg tgaaatggat cgcagaggca catggaggaa
     1981 gtgtagaagt tgtcagtaaa gaaggcgaag gaagtgagtt tgtttttcat ttaagaaaat
     2041 cgaagtcatg acaggacaat gtaaatttgt tttcgttctt taatgttatt ttaatctttt
     2101 tgggatatag taatctcatc aaagaaaata aaggcataaa ccgaagtttg gaatcgaatg
     2161 ttttcaaagt aaaagtaaat gctgatgtga aataaagaaa ggagaataga aatgaaaaaa
     2221 tttacaaaaa tagcaacggt agcagtgatg actatggtga cagcaggtgt atttgcaggg
     2281 tgtggactaa aaggcggcaa agatatagga aaagaagcgg cattggaagc ggcattggct
     2341 gatgcgggag taagagaatc agatacgacc cgtctgagag tgtcttcgga gatcgacgat
     2401 ggcaggaaag tatatgaagt gcggtttgac gtagaaggaa cagagtatga ttatgaaata
     2461 agcggaagag acggtcaaat attaagcgca gatacagagc ggatttcaac tgtagataca
     2521 acagacagag caggcacaac agatacagcg ggcacagcgg atacagcagg cacaacagat
     2581 aaagaaaaaa gcggaaattc tcaaaataat gcggaagctt tggggatcag tatagaggaa
     2641 gcagagaaga tcgcattgga aagagttccg ggggcatcgg aaaacgatat tcgcattcaa
     2701 tcagactacg atgacggaaa atataaatac gaaggcgata tcatttatga cgggaaagag
     2761 tatgagtttg aaatagatgc gtctacagga acgattcttg agtggagtga ggaacgatac
     2821 tgatcaaagg aaaattgtga ttagatgtga taaaaaagac gataaaaaat attttcggag
     2881 tatcggaata aaagaaagag agaagagaat ataattcaga aagaatgaaa aatgaatcat
     2941 agacagcgga gaagtgtatg aattatctat gggcaggcat gattttgatc ggaattatat
     3001 tcggagcgtt taacggcagg atggaagtta ttacaaatgc cgcgctcgat tcttcaaaag
     3061 aagcggtgac actttgcatt acgatgatag ggattatgtc gttttggtgt gggatcatgg
     3121 agattgcggc acaggcaggg atcatcggaa gtgcggcaaa aaaaatgcgt ccggtgatcc
     3181 gttttctttt tcctgatctg ccgaaagaac ataaagcgca ggagcatatt acaacaaatt
     3241 ttattgcgaa tatcctcggt cttgggtggg cggcaactcc ggcaggatta aaggcgatga
     3301 aagaacttga aacattggaa gaggaaaggc gggcaggaaa agtgcccggt ccggtgaggc
     3361 gcaagggagt cgcaagtaat gaaatgtgta cgtttttgat catgaatatt tcttctcttc
     3421 agttgatccc gatgaacgtg atcgcatatc gaagtcagta tggaagcgcg aacccggcgg
     3481 caatcgtagg cgcggggatt ttagctacgg tggcaagtac gggggctgca gtcatattct
     3541 gcaaaatagc ggagcaaagg cgaaagaaat ctggtataat caaggagagc agatatccgg
     3601 taaagaacag ggaatctgca taaaaaacag tatgtgtaag atcgtatccg ctgcagacag
     3661 tacgggtgtg tgaagggaga atgtatggac aaaagatata gtgaagagga aaaagataca
     3721 agagaagaat tgaaaaagga aattaaaaga gaaataaaga aacaaaatag gaaactcaaa
     3781 attaagattc cgtggaaaat tcaaaagata cttattatta tagcgattat cgcgcttgcg
     3841 ggaggcgcgg gagcgatcgg tatgaaacgc tatattactc atgaaagcca gacgaccaaa
     3901 cttgggtttg aagatatcgg ggagttggcg acacaggagg caaattgcac agaagtaaat
     3961 gttaccgaag atgtaagaaa attgttcggt attgacattc cgtttacgca gagtaaatat
     4021 atctatagtt acaatgtgat cgtaaaggcg ggatatgatt ttggcgagat tcgatatgaa
     4081 gtaaaaaaag attctatcaa agtgaaactt cctgaagtca gaatcttaag ttcgcaggtt
     4141 gatgaagatt cgtttcgtgt atatcatgag aaggaaagca ttttcaatca gatccgtctg
     4201 gaagagaata acgcggcgtt tgccaaacta aaagaacagg ccgaaaaaga cgcggtggca
     4261 aacgggcttt atgagaatgc aagaagtaat gcaaagacga tcctgaaagg atactttgct
     4321 caggcatatg atttagatga ttataaaatc acatttgaag gaggagaagg agggaataca
     4381 aatgaaaaat aaaatcttgc ttgtactcgg gatcatagcc gctgcaatcg ccatatttta
     4441 ttttatatta tttttgacgg cataggaaag agagttcgtc gagaagacgt tgattgtctt
     4501 tgctgtccat aacacaaaac cggataaatt ctccgtctaa acttttgaag gaagaacaat
     4561 cgcggatcat aagaccgttt ttgacgcagt gagtaaatac gtcaaaggcg gtcgtttctt
     4621 tttggagaag ttttacaagc agaaagttag cgtatggctt atatgtttta taagcaggct
     4681 gctcttttaa agcagagtac attttttccc gttcggaaag aatgagacgg cgggtctgtc
     4741 tgatatattc ttcatctttc agcatgagtt ctccggcaaa agcgccgagg ctgttcaaag
     4801 accagggaat ctgcttttct ttcattgctt ttaaaaacgg catgttaccc gtgataccat
     4861 aacctaagcg gactccgggc gctgcaaaaa attttgacac gcctcgtaaa accattagat
     4921 ttttgaatgt tttcgtaaaa ggtactgctg tgacgtcttc gataacaggg gcaaattcaa
     4981 cataagtctc atctatcatg acaaagatgt catgctgttc gcaaaatgta agaagacgtt
     5041 ccatatcaga tcggagaatg gcagatgacg taggattgtt cggattacat atgataagaa
     5101 aatcataact tccggcaagg agcgactgac agagaaaatc tacatttaaa ataaactcat
     5161 cagtttcttt taaaagaaac tcgttgcatt tgctatttga aagtgacaac tctctggagt
     5221 attccgaata ggttggtcca aggagaaggg tgcgctttgg ggcgcgtgtt tcgattaaaa
     5281 gagagataag ttcactcgag ccgttcccgg gcagtacgta gtctgcggga acgccgcagt
     5341 attctgaaac ggtattccga agagattgat agtcacggtc gggataagag gaaagaagat
     5401 ccaaatggga tgcaagaacc tgttttacat gggtggagat accgatagga tttacatttg
     5461 cgccgaagtt gacgatatcc tcctgattaa gatgatagta ttcacatatt ttttcgatat
     5521 cgcttccgtg gaaaacaggt tttgtattca tagcatttcc ttcctgatga ttaaaaaagt
     5581 aataataaca gtaaaatatt aacgtgcaaa aaataaagcg tttgtcttgc cgggcatctt
     5641 attaagcgtt tcgagcttct tttaatgcaa tggcaagctg atccggacaa gaggtcggtt
     5701 tgaatccgca ccggatgcct tcaatgcgcg agatcgcctc atctacatcc ataccttcga
     5761 tgaggtgaga aattccctga agattcccgc tgcatcctcc gacaaaagat acgtttctta
     5821 ctttattgtc cacgatgtca aaatgaataa gacgtgagca gacgcccttt ggtttgtatt
     5881 ccatgatttt acctcctgaa aacagatttc ttcattttta taaaaggaat tataacaaac
     5941 agaggaagaa aatacaaatt ttgtgcgtct gttttcgaga aaagtcgaca tgagtgcagt
     6001 cggttgaaaa tcgaacggga acagaaagga agaaagagaa aaacaaggga gacgtattta
     6061 tacatattac agtcagagag agaggatgct tgcagttgag aggaaaaata aaaagtatat
     6121 ctaaaacagt atgagatgat ctgcaaaaaa atgcgcggca cgaaaaaaag gagaaagaag
     6181 aaaatcggga aacctacaaa agaaaaggct gcgcggtgta gaaaaatgta gaacggtttt
     6241 tgcggacaag acagaaatcc tgtcttataa tggctacaaa cacaagcgga cgttgacaga
     6301 cggggcgtgt gaaagataaa aaaacaggag ggcttgttat gcttgagaca atgatggtaa
     6361 gttccggatc ggtcatgtat gtgatggcgg cagtcgggat acttggggtg ctggcaaaga
     6421 tcgtaaaccg gatgaccttg tacagattga taaaagcggc tggaaatatg ccgaaaagta
     6481 cgcataagct gatgaaactt gtaaaggcaa aatacgaaca tgcctgtatg gcgcatgagt
     6541 ctgtggaaaa tgtcggtgta tttgtagaaa aatatatcta tgaatacaga ggatttttgt
     6601 ttcggataca cacatggcgt cagatcgagc tgttgtctgt ttggtttgtc ggtattttag
     6661 cggtgatcgg cgcggcgctt tcttacatgg aaggcggact tacggaaacg gtttatcagt
     6721 acatagcagg cggggcggcg gaagcggtgc ttcttatggt gatcattcgt atgtcggacg
     6781 agccgtataa gataaaagcg attaaaatgt atatggtcga ttatttggaa aatgtatgcg
     6841 ctgtcagggt aagaagacag aacaggaaag aaaaagaatt tattgacgta atcccgtcag
     6901 aaaatgtaaa tggaggtgga aaagtaaaag aacaaaaaac atttgccggg gcggacgata
     6961 agaaagaagg agcgtctgcc ggtacagtac cggtgcagga gaacagggca gataaaaagg
     7021 aaaacgcaaa aggcgccgca acaatgcagg agacggagta tgaacaatgg cgggcgcagg
     7081 cagtcagaga gattttagat gagacaggga gcagtatggt agagaaacgg cttcctatta
     7141 atatcgaagg cgagccgcaa gagagaaaga aagcggaaaa gctcagccaa aatgtcggca
     7201 gaggaggggg agaacacgaa ccggacagcg ggcatccgca gatcagagag gagacgatac
     7261 ggcaaatact ggaggaattt cttgcttagg aagtgtatat ttgttaaaat aagagtatat
     7321 ggcggagagt aattctgccg gatctataaa acagacaaaa gataaatcat aaaataagta
     7381 tcagagagga tggattgtga tgagcaaagt aacatttgat tattcaaagg catgtggttt
     7441 cgtacaggat catgagatgc agtatatgag cgaactggca gagcaggcaa aggaaaagct
     7501 gcttgcaaga acgggcgccg gaaacgactt cctcggctgg atcgatcttc cggttgatta
     7561 tgataaagat gagtttgacc ggatcaaaaa agcggctgag aaaatcagag aagattcgga
     7621 agtgcttctt gtgatcggaa tcggcggatc ttatctcgga gcaagagcgg caatcgaatt
     7681 tctcggacac tcttttgcga acgttgtatc aaaagaagtg agaaaatctc cggagattta
     7741 ttatgtcggc aacagtatca gcagtacata tgtaaaagat ctgatcgatg tgatcggaga
     7801 tcgtgatttt tcaattaata tgatctccaa atcgggaacg acgacggaac cggctatcgc
     7861 tttccgcgta tttaaagaac tgttggagaa aaaatacgga aaagaagagg ctgcaaagag
     7921 gatttacgca acgacggaca aagcgaaagg agcgttgaaa aatcttgcca cagaagaagg
     7981 ttacgagaca ttcgttgtgc cggacgatgt aggcggtcgt ttctctgttc ttactgcagt
     8041 aggtttgctg ccgatcgcgg taagcggagc ggatatcgac aaattaatgg aaggagcggc
     8101 atcgggaaga aaggcggctt tggaagctga ttttgaaaag aatggcgcaa tgcagtatgc
     8161 ggctgtcaga aatattcttc ttcgtaaagg aaaaacagtg gaagtgctgg caaactacga
     8221 accgagtctt cattatgtat ccgaatggtg gaaacagctt tacggagaga gtgaaggcaa
     8281 ggatcagaaa ggaatctttc ctgcatctgt agatcttacg acagatcttc attctatggg
     8341 acagtttatt caggatggaa acagaattct ttttgagacg gttcttaatg tagaaaaatc
     8401 aagagcagag attgtgatca acgaagaacc ggtagatctt gacggattga attatcttgc
     8461 cggaaaaaat gttgatttca ttaacaagag tgcgatgaac ggaacgatcc ttgcgcatac
     8521 ggacggtaat gtgccgaacc ttatggtaaa gattccggag cagaatgagt tttatctcgg
     8581 ggaattgttt tacttctttg aatttgcatg cggagtgagc ggatatttac tcggcgttaa
     8641 tccgttcaac cagccgggtg ttgaaagtta taagaagaat atgttcgccc tgcttggaaa
     8701 accgggattt gaaaaagaaa gagaagaact gcttaagaga ctgtagtttt aaaatttctt
     8761 gttggatttc cgtaaataga gtataataat aagcggtcgg aattaccggc tgcttatttg
     8821 cattatagaa actattcaaa tatcagaaaa acagttttgg ggtgcgttat gcacggatga
     8881 tacgatcaag gagggtgcgt tatgttaaga atcggaatgt tgacaagcgg cggagactgt
     8941 caggcattaa atgcggcgat gcgcggagtt gtgaaaggaa tctgctccaa acgggacgat
     9001 gtggagatct acggttttaa agacggatat aaaggattga tctatgcgga cttcagtatg
     9061 cttacaccga aagatttttc cggcattctt acaagaggcg gaacgattct cggaacatcg
     9121 agacagccgt ttaaattgat gagagttcct gataaagacg ggctcgataa agtagaggcg
     9181 atgaagcata catatcataa gctgaatctg aactgtctta ttattttagg aggaaacgga
     9241 acgcataaaa cggcaaatct tcttcgggaa gagggattga atgtgatcac gcttccgaag
     9301 acgatcgata acgatctgtg gggaacagaa atgacattcg gatttcagag cgcggtggat
     9361 attgcgacag atacgattga cagaatccat acgacggcaa cgtcccacag ccgtgtattt
     9421 attatagagg taatgggaca caaagtagga tatgtaacgc ttcatgcagg tatagcgggc
     9481 ggcgcggata ttatccttct tccggaaatc ccttatgata tcaatgtgat cacagatgtg
     9541 atcaacagac ggtcgagaga aggaaaaccg tttacgattc ttgcggttgc ggaaggtgca
     9601 atctcgaaag aggatgcaaa actgaaaaag aaagaatata aagagaagat ctccaagaga
     9661 aaatatccgt ctatcgcata tgagctggca gagcagatcc aagaagcgac agataaggaa
     9721 gtaagaatta ccgttccggg acatacacag cgcggaggtt ctccgtgtgc atacgaccgt
     9781 gtatttgcaa ctcgggtcgg agcaaaggcg gccgagatga tactggaagg aaaatacgga
     9841 tatatggtcg ctatgagaga tggcgagacg acggcagttc cgcttgaaga agtggcggga
     9901 aaattaaaga tggtagatcc gaaatgcgga ttgatcaaag aagcgaaaac aataggtatc
     9961 agttttggcg atgaataaac gaaataaact aagaaatcag gcagaggtgt aaaacatgtc
    10021 atatacggcg ctttatagaa aatttcgccc ggcagagttt ggagatgtaa aagggcagga
    10081 tcatattatt acaacattac agaatcagat caaggcaaac cgtatcggac atgcatatct
    10141 tttctgcggt acaagaggta cgggaaaaac aacggttgcg aaaatatttg cgaaagcggt
    10201 aaactgtgag catccggttg acggcagtcc gtgcggtgag tgcgagatgt gtaagtcgat
    10261 cgcggcaggt acgtctatga atgtgattga gattgacgcg gcgtctaata acggcgttga
    10321 taatatccgt gagattcggg aagaggtgac gtaccgtccg acagaaggaa aatataaggt
    10381 ttatattatt gatgaagtac atatgctgtc tataggagcg tttaacgcgc ttcttaaaac
    10441 attggaagag ccgccggaat atgttatctt tattcttgcc acaacagaag ttcataaaat
    10501 tccgatcacg attttatcac gatgtcagca ttatgatttc aagaggatca gtattgagac
    10561 gattacggaa cgtatgcagg aattgatgga tacagagcat gtagaagcag aggataaagc
    10621 gctgcgctat atagcgaaag cggcggacgg atcgatgaga gacgcgctta gtcttcttga
    10681 tcagtgtatt gcattctatc ttgggcagaa ggtgacatac gagcatgttt tggaagtgct
    10741 gggagcggtg gatacggatg tattcagcag acttttgcgg aaagtgatca atacggatgt
    10801 gccgggtgtg ctcgacgttg tggaggaact cgtaatgcag ggaagagatc tgacgcagct
    10861 tacggcagat tttacatggt atttaaggaa cttgcttctc gtaaaaacat ctgataatat
    10921 agaggatgtt ctggatgtgt ctacggaaaa tatgatgcag ttaaaagaag aatccgggat
    10981 gatcgagaca gcagcgttat tccggtatat ccgtgttttt tcagagcttt ccgggcagtt
    11041 aaaatatgcg acgcaaaaga gaatcatgct agaggtggct ttgattaaat tatgtactcc
    11101 ggcaatggaa acgaagcagg atactatttt agagcggatc cgtgcggtgg aagaaaaagt
    11161 ggaaaaaagc atggaagctt cagcaaatat gcaggggcag atcgtctatg taaacggaga
    11221 cggagcgcag atcgcaggcg gatcggtgat taatggaaac accgagggcg attttgcagg
    11281 cgctcgtgcg cagagaccgc aaattccaga tgcgattccc gaagacgtac aggaagtcgt
    11341 aaagaatttt agaatgattg ccggagaagc ttcaggtatt ataaaggggt atctgaaaag
    11401 cgcccgctta agtctgggcg gagacaaccg gcttttgatc gttcttccgg acagtcttgc
    11461 ggcaagcgtc gtagggcggg aagatcacgt tgaagagttg gaaaaattaa tcgaagaacg
    11521 gatcggaaaa cgtgtggaag tagaagtgcg tcatgtagaa gaaggcagac attttgagga
    11581 tacattcata gatatagaac aaaagatcaa tatgaagata acggtggagg attgattatg
    11641 gcaaaaagag gcggttttcc gggtggagcg atgccgggaa atatggcaaa tcttatgaaa
    11701 caggcgcaga agatgcagcg tcagatggaa gagcagcaaa aagagcttga ggcaaaggaa
    11761 tttacggcga cggcaggcgg tggagcggtt gaagtgactg tttcgggaaa acgcgaagtg
    11821 acaaaagtaa aacttgcgga agaagtcgtt gatccggatg atattgagat gttggaagac
    11881 ttgatcgttg cggcgacaaa tgaggcgctg cgtcagatcg atgaggcgac aggttctgct
    11941 atgtctaagc tgacaggcgg catgggcggc ggaattccgg ggatgtttta aggatggaat
    12001 attacagtaa tcagatcagc cgtctgatcg aagaattttc ccgtttgccg gggattggca
    12061 gcaagtcggc gcaaaggttg gcgtttcatg tgatcaacat gccgctggat caagtggaag
    12121 gtttggcaaa atcaattatt gacgcaagga aaaatgtgcg gtattgtaaa gtgtgctgta
    12181 cacttacaga tcgggaaacc tgtccgattt gcggaaatcc ggatcgcgac aagaaaacga
    12241 tcatggtcgt tgagacaccg cgggatctgg cagcgtatga aaagacagga aaatataacg
    12301 gagtatatca tgtgctccac ggggcaatct ctccgatgct gggaatcggc ccgggagata
    12361 tccgtctgaa agaactgatg gaacggcttc agggagacgt gaatgaagtg atcattgcaa
    12421 cgaattcaag tctggaaggc gagacaacgg caatgtatat aagcaaactc ataaaaccgg
    12481 cgggaataaa ggtgagcagg attgccagcg gcgttccggt cggaggagat ttggaatata
    12541 ttgacgaggt gacgctttta agagcgcttg aaggcaggac agaactgtaa gatagagcag
    12601 aaataataaa cagaagtaat aaacagaagt aagaaagagg agacattaaa gaggagacat
    12661 tatggacaag ttaaagttga tgaaaactgc aaatgaagtc cgcaagggaa ttgtgacatc
    12721 ggttcacagt gcgaaagcag gacatccggg cggatcactt tccgcggcgg atctattcac
    12781 ttatctgtat tttgaggaat tgaatgtaga tccgaaagac ccgaaaaaag cggacagaga
    12841 tcgtttcgtc ctctcaaagg gacatacagc gccggggctt tatgccgcgt tggcagagaa
    12901 aggatttttc ccgaaagaag atctgatcac actgagacat acaggttctt atcttcaggg
    12961 tcatccggat atgaagtgca ttccgggtgt tgatatgtct tcaggttctc tcggacaggg
    13021 agtttctgcg gctgtaggaa tggcgattgc ggcaaagata tcgggtgatg attaccgcgt
    13081 atatacactt ctcggtgacg gagagatcca ggaaggtcag gtgtgggaag cggcgatgct
    13141 tgcgtctcac cacaaactgg ataaccttct tgtgatcgtg gataataata atcttcagat
    13201 cgacggtgag attacaaaag tgaactctcc gtatccgata gataaaaagt ttgaagcgtt
    13261 taattttcac gtgatcaata ttgacggaaa tgattttgat cagattgacg ctgcatttaa
    13321 agaggcaaaa tcagtaaaag gacgtccgac ggtgatcata gccaagactg tgaaaggaaa
    13381 aggagtttct tttatggaaa atcaggcggg atggcatgga aaagcgccga acgacgagga
    13441 atttaaaatc gcgatggcag atttggaaaa ggcaggtgaa gcgttatgtc agaagtaaag
    13501 aagattgcga cgagggaaag ttacggagaa acgctggtag agcttggggc ggaacatgaa
    13561 gagatcgttg tgcttgacgc ggatcttgcg gctgcgacaa aaacaggttt atttcaggct
    13621 gcatatcccg aacgtttcat caactgcggt atcgcggaaa gtaatatgat cggcgtggca
    13681 gcaggattgg cggctgcggg taaagttccg tttgcaagtt ccttcgcaat gtttgcggcg
    13741 ggacgggctt atgagcaggt aagaaattca gtcggttatc cgcatctgaa cgtaaaaatc
    13801 ggagcaacac atgccggaat ttccgtaggt gaagacggag cgacacatca gtgtaatgaa
    13861 gatattgctt tgatgcgtac gattccggga atggttgtga tcaatccgtc tgatgacata
    13921 gaggcaagag cggcggtgcg cgcggcttac gaatatgtag ggccggtgta tatgcggttc
    13981 ggacgtctgg ctgttccggt gatcaatgac agaccggatt ataagtttga acttggaaaa
    14041 ggcgttgtgc ttcgagaagg aaaagacgta acgatcattg cgacaggact tcctgtcaac
    14101 aactgtctgc aggcagcgga gaagctggca gcggaaggta ttgatgcaaa agtgatcaat
    14161 attcatacga tcaagccgct tgacgaagaa cttatcattg cggccgcgca ggaaacaggc
    14221 aaagtcgtta cagtcgaaga gcattcggtt atcggaggac ttggaagcgc agtatgtgat
    14281 gtgctttccg caaatgttcc gacaaaagtg cttaagatcg gaattaacga tgtgtatgga
    14341 gaatcaggtc cggcgcttga actgatcgaa aaatacggac ttggcataaa cggtatttat
    14401 gaaaaagtaa aagcgttcgt atcataatat tacaaaagga gatgctgttc attttgagcg
    14461 gcatctttta taatatgtcg aattcttctg acaataactg ataaaatcat tttttttatt
    14521 gtgctatcct catatcaacg ccgatagaaa cattgttgat ttttaaaata ttcagttgat
    14581 ttaggaggat gagtataaat ggaaagacag ttaccgaaaa acgtgcgtca aataggaaat
    14641 gtaagcgaca gtcctaaaat ttatgtggaa gattatgtag atacattttt tgccggattg
    14701 tgtgagaaat gtgagaaagc aggcacagac cctatggggg cttttcttgt cggagatatt
    14761 caaaacggcg aggatgaaga ttatgtttat atttatggtg cgattcagat gcatgatctg
    14821 aaaatgtcgg ataaagaata tgtgattgat gaggctgcat ggaaaaacgc atatgaagac
    14881 tgcaaacagt attttgaaga cggggaaatg ctaggatggt ttgtagtaca tcccggagcg
    14941 ccgctcattc cggaaccgga aaccgtaaag cttcataaaa aatcatttcc gaagaaaaat
    15001 accgttttca ttatgaaaga tccggtagaa aaagaagaga catattatgt acataaaatg
    15061 aatgacttgc tggagatcgg agggcattac acatattatg aaaaaaatcc gtgtatgcag
    15121 aattatatga tctccagccg gaaaaaaaat ggggcgtcag cgtcggaaac agtggaagac
    15181 agagctgcaa aggactttcg ttctttggct agaaaacgga gcgagcatca aaaaggcaga
    15241 cggccgggga gtctgatgta tgctgcggga gcttgtgtga ttttgatact tgtaattatg
    15301 ggggtatcgt tgtttaacag tttcgacagg atgaaatcgg tacaaaacac gcttggattt
    15361 ttagataaag agacagttgc gtcagatggg aaaacgtcag aagaaacaac agatgacgga
    15421 tctgttccca catctgcaga aattgaagca gtaaaaggac agacagatgc agattccgca
    15481 gatgaaaaca aaacaaagga ggaaagtgtg tcaggtcaaa aggaagacgg aaaagatgag
    15541 aaaaatgaaa atccggaaaa cagcctgtct gagagcaatg ataaaaaaga accggaggga
    15601 agcgattctg cgcagggggc agatgaacag actattggaa gcgcgaacgg tcaggacggg
    15661 gtttatattg tagagaaagg agacacgctg gcgattatca gccgtaagat atacggcgat
    15721 cttgaccatg tagacacgat ttgcaaaatg aacgggatta cggacggcaa tcttatttat
    15781 atcggacaga aattgctatt gccatgaaag tatggtaaga tgttggcgaa tagatttagc
    15841 ggaaataaaa aggacataaa acagaaatat aaagaagggg agttatatga cacaaaagaa
    15901 aaaaacggat tttaaaatag taacaccccc cgatgatcta agtgaagttc gtgctaaaac
    15961 gaagaaaata cacagggaaa aattaaaaaa gattgttgtg ccggttattc ttattgctct
    16021 tgcggtaagc ggaacatatc tgatgctcac gaataaggcg tatagtgaag ccggaacggc
    16081 ggtcaggtac tcgacagata gttcggatac aagtaattat gcccattttg caaatggaat
    16141 cgtgcgatac aatcgggatg gcgttgtttt tttaaataag aaaaacgaag aaaagtggat
    16201 tcagtcgaca caactgaaaa atccgatcat tgaggtgaaa gaaaa
//
LOCUS       DBMDXF010000018        15881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1105_length_15881_cov_2.081496, whole genome shotgun sequence.
ACCESSION   DBMDXF010000018 DBMDXF010000000
VERSION     DBMDXF010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1105_length_15881_cov_2.081496"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(501..4991)
                     /locus_tag="ACZPD4_01515"
     CDS             complement(501..4991)
                     /locus_tag="ACZPD4_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012931.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIR1377791.1"
                     /translation="MPEAKTARYVRLYIDGYTANAADDQTNWRSVSLYDFQIYANEIP
                     DTVLPDENYCLEGTAEASNFEEVQSEPGKQGPAKAIDGDLTTRWATESDGKGTTARTL
                     TVKLPAAQWVEYVKIDWEMPASGVPTPKKYTIEAAEKEGGEFTVVHTNSEQVTKAEQI
                     IKLDKPVWAKELKLNVTEYGAATGTWYNVSVAEFGAYAMKPESGVIDENATAKQIADK
                     LAAPTVKADKTGLEWQDVPEGVTVEFLADYEQVVGRDGKIYQPLRETTVKGIYKVTKG
                     EETAEGAREHTVTIPGKYDNAGTNAKPVVIPELAEWYGGTEAGSVKIGEGTKIVYKDA
                     AFKAAAEALAADYKAEYGVDLQVADSGEDAGDIVFTKDDKNGLGEEGYIMEMDDKVNV
                     KAEQAQGAYWSTRSILQIVKLNNGEIPKGITKDYPKFKVRSFSLDVARKPASLESLED
                     FVDAMAYYKMNDFQVHLNDNLIFYENFESAEVARERAYTGFRLESDIKAGGENKKDLT
                     NEDLFYTKEDFRNFIEESEAQGVSIVPEIDAPGHSGAFTKVRPDLMLPQNQAVNGNAK
                     RAGEQFDLSGDVNSKDSQYGKSLSFVQGVWDEYLTDNMFDDSMTVHIGTDEYYGEENA
                     FRHFSDDMIKYIQGKDRTVRMWGSLTQIDGDGTVPVKSENVQLNVWNTGYSNPKQMYN
                     DGFDLINTLDGSLYMVPNGSGSRGGYGDYLRTEDLYKNWIPNNMGGTVIPAGSDQMLG
                     STFAIWNDNIDTAAQGISEVDNFERFEDALPILASKGWGEGEDLEFAAVKEKAEKLGD
                     APGSNPYYEETADKDGKYMSYGFDNKEDSSENNRDIKETKNADIDGALKLKGGESYAS
                     TPIEKLAVGNALSFDVTLTEEARAGQILFEADTPGGEDYVHDIRIMDDGRVGFRRELY
                     DYYFDYKLPVGEKVHMEIVTDTLKTVLIVDGKEYQATGIYINRQTDNTLRKQGIKNAT
                     LLLPVQRIGSKTNSIVGTIDNVEVKTADPISSKDEYNKAAWTKVSVDTETNIADSKTE
                     GLFTYAFDGKSNTIWHSNWKDGKSDKLKPQGSFDEIGGVINLGQKYTINQFSFTPRQG
                     NASGQVTKADLYVKANEGDEWVKVAENTEFAADASKKTFYFDEQEVQFVKFVAKTSND
                     GWVAVSEFDVDNEKVKEMTVYAAAQKGGKVTSSAKDGKVMQHENVTVTATPNKEYKFA
                     GWYDILTGEKVSDDAEYTFAVEMNTSLIAHFTKNGETPDPQPKEWTVTIDGTGHKVKD
                     GETLTVEEPSKPGHAFAGFYLVGTDTAVDFTKPITSDMNVESRFTQYKVTAVESENGT
                     ITVSEMNDKGEVTVTAKANDGYIFKGWKVDGKETEALGTPYTFVPVKDTTVEAVYEKK
                     DDTKVYHTVIINGQTVTVEDGKTLDRPADPVKEGYKFIGWFVDGKEFDFNTPITGDLK
                     IEARFEKLPTTDKNDKNDKNDKVEGAVQTGDTANAVPWIVCLGAAAGAGVVVLKSRKK
                     RS"
     gene            complement(5002..5433)
                     /locus_tag="ACZPD4_01520"
     CDS             complement(5002..5433)
                     /locus_tag="ACZPD4_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1377792.1"
                     /translation="MKKLTRAGVGALAGVMVFGLAAPLAPLSVEAADEVKNLAIGSTA
                     TVNDKETDYWGADKAVDGIVNRTAAKRDQSRWATNVGGDADAKWLKIDLKEAKTFQSF
                     VLAWERRNITGYKIQISQTGEDNSWEDVYTKTGASHISQYK"
     gene            complement(5914..6606)
                     /gene="rplA"
                     /locus_tag="ACZPD4_01525"
     CDS             complement(5914..6606)
                     /gene="rplA"
                     /locus_tag="ACZPD4_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1377793.1"
                     /translation="MKRGKKYIEAAKLIERGNLYEKEEAVALVKKAATAKFDETIEAH
                     IRTGCDGRHADQQIRGAVVLPHGTGKKVRILVFAKDAKAEEAKAAGADFVGGDELIPK
                     IQNEGWLDFDVVVATPDMMGVVGRLGRVLGPKGLMPNPKAGTVTMDVTKAINDIKAGK
                     IEYRLDKTNIIHVPIGKASFTEEQLADNFQTLIDAINKARPSAVKGQYLKSITLTSTM
                     GPGVKVNPMKLA"
     gene            complement(6765..7190)
                     /gene="rplK"
                     /locus_tag="ACZPD4_01530"
     CDS             complement(6765..7190)
                     /gene="rplK"
                     /locus_tag="ACZPD4_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1377794.1"
                     /translation="MAKKVEGYIKLQIPAGKATPAPPVGPALGQHGVNIVEFTKQFNA
                     RTADQGDLIIPVVITVYNDRSFSFVTKTPPAAVLIKKACKIQSGSGVPNKNKVATITK
                     AQLQEIAELKMPDLNAATVESAMSMVAGTCRSMGVVVEE"
     gene            complement(7242..7760)
                     /gene="nusG"
                     /locus_tag="ACZPD4_01535"
     CDS             complement(7242..7760)
                     /gene="nusG"
                     /locus_tag="ACZPD4_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1377795.1"
                     /translation="MSEAKWYVVHTYSGYENKVKANIDKAIENRHLEDQILEVRVPME
                     EATELRNGVQKAVQRKMFPGYVMIHMVMNDDTWYVVRNTRGVTGFVGPGSKPVPLEEH
                     EMENLGIQRDNVIVNFGVGDMVVVLSGAWEGTVGTVQSMNEQKKSLSINVELFGRDTP
                     VELSFSEVKKMD"
     gene            complement(7786..>7926)
                     /gene="secE"
                     /locus_tag="ACZPD4_01540"
     CDS             complement(7786..>7926)
                     /gene="secE"
                     /locus_tag="ACZPD4_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1377796.1"
                     /translation="FKKVIWPDKKTLARQTTAVVAVSVILGALIAVIDVILRYGIDLL
                     IK"
     assembly_gap    7927..7936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8012..8161)
                     /gene="rpmG"
                     /locus_tag="ACZPD4_01545"
     CDS             complement(8012..8161)
                     /gene="rpmG"
                     /locus_tag="ACZPD4_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1377797.1"
                     /translation="MRTRITLECTECKNRNYNMTKDKKAHPDRMETKKYCRFCKSHTL
                     HKETK"
     gene            complement(8336..9478)
                     /locus_tag="ACZPD4_01550"
     CDS             complement(8336..9478)
                     /locus_tag="ACZPD4_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1377798.1"
                     /translation="MDLNKENMKKMRELIVFTIILLIALWNYKLLFEWIGFAFGIVTP
                     FLIGGGIAFVLNVPMSFFEETLFGNKRVKEKKLAKRLARPLSLVLTIVAVIGVVVLVM
                     FVVIPELAQTVLSLGKTIQAFVPEAQHFLEELFTDNSEIRAWLNNLDVDLDKLLDSVI
                     AFFRNGAGNVLNSTVYAIGSIVNGVTTFVIAFVFACYILLQKEKLGVQVKKVMHAFLK
                     EKWEKRCLKVCTLTSKTFSNFLTGQCLEALILGSMFFVAMSLFKMPYALLVGVLVAFT
                     ALIPIFGAFIGCVVGAFLILMVDPLQALFFVIMFLVLQQIEGNLIYPKVVGNSVGLPS
                     IWVLAAVTIGGSLMGVVGMLIFIPLVSVVYTLFRECVYKRLEQKQR"
     gene            complement(9526..10869)
                     /gene="glmM"
                     /locus_tag="ACZPD4_01555"
     CDS             complement(9526..10869)
                     /gene="glmM"
                     /locus_tag="ACZPD4_01555"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1377799.1"
                     /translation="MGKYFGTDGFRGEANVVLTVEHAFKVGRYLGWYFGQDHKARIVI
                     GKDTRRSSYMFEYALAAGLTASGADAYLLHVTTTPSVSYVVRTEDFDCGIMISASHNP
                     FYDNGIKVINSAGHKLEAEVEEKIEAYIDGEIGELPLATKEKIGRTVDYAAGRNRYIG
                     HLISVATRSFKDMRVGLDCSNGSSSAIAKSVFDALGAKTYVINNEPDGTNINTDCGST
                     HIHVLQEFVKEKKLDVGFAYDGDADRCIAVDENGKVIDGDLILYICGKYLMEQGRLEG
                     NTIVTTVMSNLGLYKACDKIGMKYEQTAVGDKYVYENMLKNGYILGGEQSGHIIFSKH
                     ARTGDGILTSLLIMEVILEKKQSLGTLAGEVKIYPQLLKNVRVTDKKTARENPAVQEA
                     VQKAADELGSDGRILVRESGTEPVIRVMVEAATDEICEKYVNSVVKVIEDQGLTE"
     gene            complement(11329..12249)
                     /gene="tsf"
                     /locus_tag="ACZPD4_01560"
     CDS             complement(11329..12249)
                     /gene="tsf"
                     /locus_tag="ACZPD4_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR1377800.1"
                     /translation="MAITAGMVKELREMTGAGMMDCKKALSETNGDMDAAVEFLRKNG
                     QAKAEKKAGRIAAEGIVKTVVKDNKAAIVEVNSETDFVAKNDEFQGFVETVVNQIVDS
                     EAADMDAFMAEAWAADTTKTVKDALVEKIAVIGENLNIRRFEKVAADNGCVVSYIHGG
                     GRIGVLVVADTDVVNDDIKACLKNVAMQVAAMSPKYVSRDEVSQEYMEHEKEILLAQA
                     KKENPEKPENIIEKMIIGRLNKEMKEICLLDQVYVQDSDLTVAKYVDKVAKENNANVT
                     VKKFVRFETGEGLEKKVDDFAAEVAAQAGM"
     gene            complement(12431..13177)
                     /gene="rpsB"
                     /locus_tag="ACZPD4_01565"
     CDS             complement(12431..13177)
                     /gene="rpsB"
                     /locus_tag="ACZPD4_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR1377801.1"
                     /translation="MGVISMKQLLEAGVHFGHQTRRWNPKMAPYIYTERNGIYIIDLQ
                     KSVGMVDDAYKAVSDIAAEGGTILFVGTKKQAQDAIKTEAERCGMYYVNERWLGGMLT
                     NFKTIQSRIKRLKEIETMSEDGTFDVLPKKEVIELKKEWAKLEKNLGGIKDMKRLPDA
                     IFIVDPKKERICVQEAHTLGIPLIGICDTNCDPEELDYVIPGNDDAIRAVKLIVSKMA
                     DAVIEANQGATDEEYYEAETAVEAEEAVEE"
     gene            complement(13306..14109)
                     /gene="cobK"
                     /locus_tag="ACZPD4_01570"
     CDS             complement(13306..14109)
                     /gene="cobK"
                     /locus_tag="ACZPD4_01570"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HIR1377802.1"
                     /translation="MKKDKIWIFAGTTEGRLLTRYASQMKMKCCVSTATEYGKSVLGE
                     NEGIRVIPGRMDQSKMRAFIRENNIGLAVDATHPFARQVTENICAACEEEKIKYVRCL
                     RERETNDPADHAGETSDGRPISAASVEEAVEYLKKTGGNVLISTGSKELHLYTRIKDY
                     KERCFARVLSTQEAVAEAVRLGFEGKHLIAMQGPYSEELNLAMLKHINAAYFVTKESG
                     GAGGFEEKKKAAQKAGAELIVIARPKEEGKSLQEVKKLMEEFLAKENLL"
     gene            14329..15120
                     /locus_tag="ACZPD4_01575"
     CDS             14329..15120
                     /locus_tag="ACZPD4_01575"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1377803.1"
                     /translation="MIKLIASDLDGTLLQNEAQELTPRAIELIRALTQKGVHFVAASG
                     RQYANERLLFHEIKDDISYIAENGSLCIHQGKVISRGLIEDDLAYRILRELKKEPGFE
                     IVVSREDTCLIENNNSQFANHIVNVMHNTTEIVDDLLTVKGPFLKIAFANMTSRRTTD
                     CLKELQEKFSSEIKVVTSGNIWIDFIVPGCNKGSALRNLMDLFGITPEECVAFGDQYN
                     DVEMLETVGMGYAMSNAAPGIAGHAKYITDSVEEVLEDILAGLDL"
     gene            complement(15117..15848)
                     /locus_tag="ACZPD4_01580"
     CDS             complement(15117..15848)
                     /locus_tag="ACZPD4_01580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR1377804.1"
                     /translation="MERAHKYEFEGKVRYSEIDHRATMTLPALINYFQDCSTFQSEQL
                     GYGMDVLKKEKKAWVLAYWQVIVKRYPKLCERITVGTFASGFKGMFGNRNFYMKDESG
                     EQIACANSIWVYMDVEKGKPVRVDKEQAEAYGIDEPLEMPYEDRKIEEAESYEEKAPF
                     PVRKYHIDTNEHVNNCQYVQMALEVLPRDILVSQVRVDYKKSAVLGDKIFPETAQKKD
                     RVIVRLCDEDRKPYAVIEFKTGCRS"
BASE COUNT         3806 a         4119 c         2751 g         5195 t
ORIGIN      
        1 tcttttttaa gaccttctct taaaaatcag tatacgaaaa aaagaactgt tctacaagac
       61 tatccacgca aaaactgttg ttgtattgta tcgggtaatt ttgagggaat tcctcagacc
      121 ggcgaaactg ccggtatctt tcacagatag aagttctgtg agtgaattta tcatagcata
      181 tctatccttc aaaaacaata atatttttca ctttactatt aaaaaacacc tacatgaggt
      241 gaagagttta tttccacttt gaacaaagta gattgtagtg tttcacttat aaagaaacac
      301 agaaccacag ggaatgtcag aaggcgcatt tcaggattgt acatagccgc caaaatctcc
      361 ggcgttcccg cactctgacc tatcacctat atgaaaactg ccgacaggat tacctgtcgg
      421 cagaaaaact gtatgatact attttatttg ttatgctttc aagatcaatt atttatcaca
      481 ttctgaaaac agtttttaaa ttaagaacgt tttttgcgtg atttcaacac cacaacacca
      541 gctcctgcgg ctgcgccgag gcatacgatc cacggtactg catttgctgt atcgcccgtc
      601 tgaactgcgc cttcaacttt atcgtttttg tcgttcttat cattcttatc agtagtcgga
      661 agcttctcaa aacgagcctc gatcttcaga tctcccgtaa tcggagtatt gaaatcaaat
      721 tcttttccat ccacgaacca accgatgaac ttatatcctt cttttacagg atcagcagga
      781 cgatcaagcg tcttgccatc ttctaccgta actgtttgtc cgttaatgat tactgtatga
      841 taaacttttg tatcatcttt cttctcatat acagcttcaa ctgttgtatc tttcaccggc
      901 acaaatgtat aaggtgttcc aagagcctct gtctcttttc catctacttt ccaaccttta
      961 aagatatatc cgtcatttgc ttttgctgta acagttactt cacctttgtc attcatctcg
     1021 ctgactgtga ttgttccgtt ctcactctct actgctgtaa ctttatactg tgtaaagcgt
     1081 gattcaacat tcatatcaga tgtgatcggt tttgtgaaat ctacagccgt atctgttcca
     1141 acaaggtaga accctgcaaa cgcatgaccc ggtttagacg gctcttctac tgtcagtgtt
     1201 tcgccgtctt ttactttatg tcctgtcccg tcgatcgtta ccgtccactc tttcggctgc
     1261 ggatccggcg tttcaccgtt tttcgtgaaa tgagcgatca aagatgtatt catttctaca
     1321 gcaaatgtat attccgcatc atcagatacc ttttctcctg taagaatatc ataccatcct
     1381 gcaaacttat actctttatt cggagtcgcc gttacagtaa cgttctcgtg ctgcattact
     1441 tttccgtctt ttgcgctgct tgtaactttt ccgcccttct gagccgcagc ataaactgtc
     1501 atttctttta ccttttcatt gtcaacatca aattcagata cagctaccca accgtcatta
     1561 gatgtttttg caacgaattt tacaaactga acttcctgct cgtcaaagta gaatgtcttc
     1621 ttagatgcat cagcggcaaa ttctgtattt tcggcaactt ttacccattc gtcaccttca
     1681 tttgccttca catataaatc agcttttgtc acctgaccac ttgcatttcc ctgtcttgga
     1741 gtgaaggaga actgattgat cgtatatttc tgaccgagat tgatcactcc gccgatctca
     1801 tcaaaacttc cctgcggttt cagcttatcg ctttttccat ctttccaatt tgaatgccag
     1861 attgtatttg attttccgtc aaatgcatat gtgaaaagac cttctgtttt actatcagca
     1921 atattggttt ctgtatcaac agatactttt gtccatgccg ctttattata ctcatctttt
     1981 gacgagatcg gatctgctgt ctttacttct acattatcga tcgtgccgac gattgaattc
     2041 gtctttgatc cgatacgctg aacaggcaga agcagtgttg catttttaat cccctgtttt
     2101 ctgagcgtat tatcagtctg tctgttgatg taaattcctg ttgcctgata ttcttttccg
     2161 tctacgatca acacagtttt caacgtatct gtcacgattt ccatatgaac tttctcgcca
     2221 accggaagtt tataatcgaa gtagtaatca taaagctctc ttctgaatcc aactcttccg
     2281 tcatccatga tgcggatatc atgtacataa tcttctccgc ccggtgtatc tgcttcaaac
     2341 aggatctgtc ctgctcttgc ttcttcagtt aatgtaacgt caaatgacag ggcgtttccg
     2401 actgcaagtt tttcaatcgg tgtcgacgca taactttcgc cgcctttcag tttcagcgcg
     2461 ccgtcaatat ctgcattctt tgtctctttg atatctctgt tgttttcaga cgaatcttct
     2521 ttattgtcaa aaccgtaaga catatacttg ccgtctttat ctgccgtttc ttcatagtac
     2581 ggattgcttc ccggagcatc tcccagtttc tccgcttttt cttttactgc ggcaaactca
     2641 agatcttcac cttcgcccca gcctttgctt gcaagaatcg gaagcgcatc ttcaaaacgc
     2701 tcaaagttat caacttctga gattccctgt gcagcagtgt caatattatc gttccaaatc
     2761 gcaaacgtac ttccgagcat ctgatcagat cctgccggaa ttactgttcc gcccatatta
     2821 ttcggaatcc aatttttgta aaggtcttct gttctcagat aatcgccata tcctcctctg
     2881 cttccgcttc cattcggaac catatagaga gaaccgtcca gtgtattgat aagatcaaat
     2941 ccatcgttgt acatctgttt cggattggag tatcctgtat tccatacgtt caactgtaca
     3001 ttttcgcttt ttaccggaac tgttccgtct ccgtcaatct gcgtcaaact tccccacata
     3061 cggactgtac ggtctttacc ttgaatatat ttgatcatat catcggagaa atgacggaaa
     3121 gcattctctt caccataata ttcatctgtt ccgatatgta ctgtcatact gtcgtcgaac
     3181 atattgtctg tcagatattc atcccacaca ccctgaacaa atgaaaggct cttaccatac
     3241 tgactgtctt tggaattcac atcaccgctt aagtcaaact gctcgcctgc acgttttgca
     3301 tttccgttga cagcttgatt ctgcggaagc atcagatcag gacgaacttt tgtaaatgca
     3361 cccgaatgtc ccggagcatc aatttccggc acaatactta ctccctgcgc ttcagactct
     3421 tcgataaagt ttctaaagtc ttcctttgta tagaaaaggt cttcgttagt cagatctttt
     3481 ttattttctc cgcctgcctt aatatcggac tcaagacgga atcctgtata cgccctttct
     3541 cgagcgactt ctgcgctttc aaaattctca tagaagatca agttatcatt caaatgaacc
     3601 tgaaaatcat tcatcttgta gtatgccata gcgtctacaa aatcttccaa tgactcaaga
     3661 gatgccggtt tacgggcaac atccaagcta aagcttctta ctttgaactt cggatagtct
     3721 tttgtaatac ctttcggaat ctcaccgtta ttcagtttca cgatctgaag aatggaacgt
     3781 gtcgaccagt aagctccctg cgcctgctca gcctttacat ttactttatc gtccatctcc
     3841 atgatatagc cttcttcacc gagtccgttt ttatcatctt ttgtaaagac gatatcgcct
     3901 gcatcttctc cgctgtctgc aacctgaaga tcaacgccgt actctgcttt ataatctgct
     3961 gcaagagctt ctgccgctgc cttaaacgcg gcgtctttat aaacgatctt tgtgccttca
     4021 ccgatcttaa cagatccggc ctctgttccg ccataccact ctgccagttc cggaattaca
     4081 accggtttcg catttgttcc agcgttatca tatttacccg gaatcgtaac tgtatgttct
     4141 cttgcgcctt ctgctgtctc ttcacctttt gttactttgt aaattccctt tacagttgtc
     4201 tctctaagcg gctgatagat cttgccgtct cttccgacaa cctgttcgta atctgcgagg
     4261 aactctacag taactccttc cggcacatcc tgccactcta atccggtttt gtccgccttt
     4321 acagtcggag ctgccagctt gtccgcaatc tgttttgctg ttgcattctc atcgatgacg
     4381 cctgactccg gcttcatcgc atatgcgccg aattctgcca cagaaacatt ataccaagtt
     4441 cctgtcgcag cgccgtactc cgttacgttt aatttcagtt cttttgccca tacaggtttg
     4501 tctaacttga taatctgctc ggcttttgta acctgctcgc tgtttgtatg aactacagta
     4561 aattcgccgc cttctttttc tgccgcttca atcgtatatt tcttcggagt aggaactccg
     4621 cttgccggca tttcccagtc gatcttcaca tattctaccc actgcgctgc cggaagcttc
     4681 acagtcagcg tacgggctgt cgttcctttt ccatcgcttt ctgttgccca gcgtgttgtt
     4741 aaatctccgt caatcgcttt tgccggacct tgttttcccg gctcgctttg aacctcttca
     4801 aaattgcttg cttctgctgt tccttccaga cagtagtttt catccgggag aaccgtatca
     4861 ggaatctcat ttgcatagat ctggaagtca taaagagaaa ctgatcgcca atttgtctga
     4921 tcatctgccg cattcgccgt atatccgtca atatataaac gtacataacg tgctgttttt
     4981 gcctctggca aatgaatatt ttcatttata ttgagaaata tgacttgctc ctgtttttgt
     5041 atagacgtct tcccatgaat tatcttcgcc tgtctgtgaa atctggattt tataaccggt
     5101 aatatttctt ctttcccatg caagaacaaa cgactggaat gtttttgctt ctttcaaatc
     5161 aatcttcagc cactttgcat ctgcatctcc gcctacgttc gttgcccaac gggactgatc
     5221 tctttttgct gcagttctgt taacaatacc gtcaacagct ttatccgcgc cccaatagtc
     5281 tgtttcttta tcgttgactg ttgctgttga accaatcgcc agatttttca cttcatctgc
     5341 cgcctcaaca cttagaggtg caagcggagc tgccaatccg aagaccatta ctccggcaag
     5401 cgctcccacg ccggcgcgcg ttaacttttt cattttcctt cccatttttt acctctctgc
     5461 tttcttaaaa tttttaacga gagaaaacac gttttcgtac ttctctcgtt acttctgcta
     5521 ataaacttcc agtaaaaagt ttatatcctg atttagtcag agtcgatata aaaaaccaac
     5581 tcgcctctag cgactctctc attattatag tttttttcat cataaaactc aatatttttt
     5641 atcactacat aatttttttg tgatttttaa caatatttcc tttttcatcc tatgaaaaca
     5701 gcctttccgg gcgcatcttc tcttcaaaaa tttgtcaatt ttactatttt tactttttta
     5761 cacatgtgtt tcatattcac atactttaaa acaaataagc gatcgccaag tttctgggca
     5821 tatccggact ttcatccggt ttctataagc ttcctataaa acaaacgaga cagcacatac
     5881 tatgatatgc tgtctcgctg tatattactt taattatgca agtttcatcg gattaacctt
     5941 cacgccaggt cccattgtag atgtaagagt aatgctcttc aaatactgtc ctttaaccgc
     6001 tgacggtctt gccttattga ttgcatcgat aagcgtctgg aagttgtccg ctaactgttc
     6061 ctctgtaaag gatgctttac cgatcggaac atggataatg tttgttttgt caagtctgta
     6121 ctcgatctta ccggctttga tgtcgttgat cgcttttgtc acgtccatcg taactgttcc
     6181 ggctttcggg ttcggcataa gacctttcgg tccaagtaca cgtccgagac gtcctacaac
     6241 acccatcata tccggtgtag ctacaactac gtcaaagtca agccatcctt cgttctggat
     6301 cttcggaata agctcgtctc ctcctacaaa atcagctcct gctgcttttg cttcttctgc
     6361 ctttgcgtct tttgcaaata caagaatgcg aactttttta cctgttccgt gcggaagaac
     6421 aaccgcgccg cggatctgct gatctgcatg acgtccgtca caacctgttc tgatgtgagc
     6481 ctcgattgtc tcgtcaaact ttgctgttgc tgcttttttc actaatgcta ccgcttcttc
     6541 tttttcgtag aggtttcctc tctcgatcag ttttgcagct tcgatatatt tctttcctcg
     6601 tttcatttta caataacctc cttgtggtat ttgcgggaac gtgcccctcc cacttctttc
     6661 aaatcacttg atgtttgttt caatgcatca cggatgttta cattctgccc ttcaaaaccg
     6721 attcatcctc accgcgcttt gaatatgcag acgaaactct gtgcctactc ttctactact
     6781 acacccatag aacggcatgt tcctgctacc atactcatag cagactcaac tgttgccgca
     6841 ttaagatccg gcattttcag ttccgcgatc tcctgaagct gagcttttgt gatcgtcgca
     6901 actttatttt tgttcggtac gcctgatccg gactggatct tgcaagcttt tttaatcaaa
     6961 actgctgccg gcggtgtctt tgtaacgaag ctgaaacttc tgtcattgta tactgtgatg
     7021 acaaccggaa tgatcagatc gccctgatct gctgttcttg cgttgaactg ttttgtaaat
     7081 tcaacgatat taacgccgtg ctgtccaaga gccggaccaa ccggcggtgc cggagttgcc
     7141 ttgccggcag gaatctgcaa tttgatataa ccttctactt tttttgccat ttcaattacc
     7201 tccttgtggt attgtcgggg acaagcccct cccacttact tttaatccat ttttttcact
     7261 tctgaaaaac taagttcaac cggtgtgtca cggccgaaca gctcaacatt gatcgacaga
     7321 ctcttcttct gttcgttcat gctctgcaca gttcccactg tgccttccca tgctccgcta
     7381 agtacgacaa ccatgtcgcc gactccgaaa ttcacgataa cattatctct ctggatgccg
     7441 agattttcca tctcatgctc ctcgagcgga accggttttg atcccggtcc gacaaagcct
     7501 gtcactccgc gagtatttct gactacatac catgtatcgt cattcatgac catatggatc
     7561 atcacatacc ccggaaacat cttgcgctga accgcttttt gaacaccgtt tctcaactct
     7621 gtcgcttctt ccataggaac gcgtacttca agaatctgat cttcaagatg acggttctcg
     7681 atcgccttat caatattcgc tttcactttg ttttcatacc ctgaataagt atgaactaca
     7741 taccattttg cttctgacat aaaatcacct ttccgctgcg gtcaactatt ttatcaataa
     7801 gtcaataccg tatcttagga ttacgtcgat caccgcgatc aacgctccga ggattacgga
     7861 aacagcaaca actgccgtgg tctgtcttgc aagtgttttc ttatccggcc aaataacctt
     7921 tttgaannnn nnnnnncgct tgaagtcctt tgaaccagct ctttttctga gtcttttctg
     7981 tttcactcat aattccactt ccccctgacg actacttcgt ttctttgtgc agtgtgtgtg
     8041 atttgcagaa tctgcagtat tttttagttt ccatgcgatc cggatgagct ttcttatcct
     8101 tcgtcatgtt gtagttacgg tttttacatt ccgtacactc caatgtaatt cttgtgcgca
     8161 caacttccac ctccgcttta attattcact acttgttgct gacggctgcg accccgtcaa
     8221 aatttaggca taaaaaaaag acctacttcc attcgccgta gtattgtacc acgaaaaacg
     8281 gaagtaagtc aagatgttta ttaaattcca ttcatttcta catgaaatat atgaattatc
     8341 tctgtttttg ttccagtctt ttatatacac actctctaaa cagcgtatat acaaccgaca
     8401 ctaacggaat aaagatcagc atccccacaa cgcccatcaa actgccgccg atcgttactg
     8461 ccgcaagcac ccatatggaa ggcagaccga ccgaatttcc cacaactttc ggataaatga
     8521 gatttccctc aatctgttga agcacgagaa acataatgac aaaaaacaat gcctgcagcg
     8581 gatcgaccat caaaatgagg aacgctccta caacacagcc gataaaagcc ccaaatatcg
     8641 gaataagcgc tgtaaatgcc acgagcacac cgaccagaag cgcatacggc atcttgaaaa
     8701 ggctcatcgc cacaaaaaac atcgacccga gaataagcgc ctcaagacat tgtcccgtca
     8761 aaaaattaga aaatgttttg gaagtcagtg tacacacttt cagacatctt ttttcccatt
     8821 tttctttcaa aaacgcatgc atcacttttt ttacctgcac gccgagtttc tctttttgaa
     8881 gcaaaatata acatgcaaat acaaacgcga ttacaaatgt tgtcacaccg tttacgatag
     8941 aaccgatcgc atataccgta gaattcagca cgtttccggc gccgtttcta aagaaagcga
     9001 tcacgctgtc caaaagctta tccaaatcta cgtccagatt attcagccac gcgcgtattt
     9061 cactgttgtc tgtaaataac tcttccagaa aatgctgtgc ctccggtaca aatgcctgta
     9121 ttgtctttcc aagcgaaagc actgtctgcg ccagctccgg aataacgacg aacatcacca
     9181 gaacgacaac tccgatcact gccacgatcg taagcacaag actcaacggc cgagccagcc
     9241 ttttcgccag tttcttttct tttacccgct tatttccaaa caaagtttct tcaaaaaaac
     9301 tcattggcac gttcagcaca aaagcgattc ctccgcctat aagaaatgga gtaacaatgc
     9361 cgaacgcaaa gccgatccac tcaaacagca gtttatagtt ccaaagtgca atgagaagaa
     9421 tgatcgtaaa aacgatcaat tctctcatct ttttcatatt ttctttattc aaatccatat
     9481 atttttaatt cccgatctct atttttaccc gcgaaatcgt aatgcttatt ctgtcagccc
     9541 ctgatcttcg atcaccttca caacgctgtt tacatacttt tcgcatattt catcagttgc
     9601 cgcttcgacc attacacgga tcacaggctc tgtaccgctt tcgcgaacaa ggatacgtcc
     9661 gtcgcttcca agctcgtcag ccgctttttg aactgcttcc tgaactgccg gattttctct
     9721 cgccgtcttt ttatctgtga cacggacatt tttcaaaagc tgaggataga tcttcacctc
     9781 tcccgcaagt gtgccgagag actgtttttt ctcaaggatt acttccataa tcaaaagtga
     9841 ggtaaggatg ccgtcgcctg ttcttgcatg tttactgaaa atgatatggc ctgactgctc
     9901 accgccgaga atataaccgt ttttcagcat attctcatag acgtacttat cgccgaccgc
     9961 cgtctgctca tatttcatac cgatcttatc acacgctttg tacagtccca agttagacat
    10021 taccgttgtt acgatcgtgt tcccctcaag acggccctgt tccataagat attttccgca
    10081 tatatagaga atcaagtctc cgtctatcac ttttccgttt tcatctacag caatacatct
    10141 gtctgcatct ccgtcataag cgaatccgac gtcaagcttt ttctctttca caaattcctg
    10201 aagaacgtga atatgtgtag atccgcagtc tgtattgatg ttcgtaccat ccggttcatt
    10261 attgataaca tatgttttag ctccgagcgc gtcaaatacg cttttcgcga ttgcagatga
    10321 acttccgtta gaacaatcca atccgactct catatctttg aaagaacgtg ttgctactga
    10381 gataagatgg ccgatataac ggtttcttcc cgctgcataa tcgacagtac gtccgatctt
    10441 ttcttttgtc gccagcggaa gctctccgat ctcaccgtct atatacgcct caatcttttc
    10501 ttctacttct gcctctaatt tatgtcctgc gctgttgatc actttgatcc cgttatcata
    10561 aaacggatta tgacttgcag aaatcataat accgcagtcg aaatcttccg ttctcaccac
    10621 atatgaaaca ctgggggttg tcgtaacatg aagaaggtac gcatccgcgc ctgacgctgt
    10681 caatcccgcc gcaagagcat actcaaacat atagctgctt cttctcgtat cttttccgat
    10741 cacgatcctt gccttatgat cctgtccgaa ataccagccg agataacgtc ctaccttaaa
    10801 tgcatgctct actgtcagca caacattcgc ctcaccacga aagccgtccg ttccaaaata
    10861 ttttcccata atgattatcc tctttgttct tttataatat ttatgctgca aaagaatctt
    10921 ctactctctt ttgtgacata aacacattat attaccatta cgaaaaagtt acaagtattt
    10981 cttatatgtt acatttttct ttttccgcac aagcgcttag ttcgattccg cttcgctaca
    11041 tctcactacc gtgcgtctcg cttcgtccgc acaagcgctt agttcgattc tgcttcgcta
    11101 catctcacta ccgtgcatct cgcttcgtcc gcacaagcgc ttagttcgat tctgcttcgc
    11161 tacatctcac taccgtgcgt ctcgattcgt ccgcacaagc gcttagttcg attccgcttc
    11221 gctacatctc actaccgtgc gtcgcacggt atatacaaaa agaggattcg atatgagtaa
    11281 actcatatcg aatcctcttt ttgtatatgt cgcgcttgtg ctaaacgctt acattccagc
    11341 ctgtgctgct acttctgctg cgaaatcgtc aacttttttc tcaagtcctt cgcctgtctc
    11401 gaaacgaacg aacttcttaa ctgttacgtt agcattgttt tcttttgcta ctttgtcaac
    11461 gtattttgca actgtaaggt cgctgtcctg aacgtatacc tgatcaagaa ggcagatttc
    11521 tttcatctct ttgttgagac gtccgatgat catcttctca atgatatttt ccggtttctc
    11581 cgggttctct ttcttagcct gagcaagaag gatctctttt tcatgctcca tgtattcctg
    11641 agaaacttca tcacgggata catatttcgg agacatagca gcaacctgca tagctacatt
    11701 cttaaggcat gctttgatgt cgtcattcac aacgtctgta tcagccacta cgagaactcc
    11761 gatacgtccg ccgccgtgga tgtaagatac aacgcatccg ttgtcagcag caactttctc
    11821 aaatcttctg atgttaaggt tttctccgat cactgcgatt ttctctacga gagcgtcttt
    11881 tacagttttt gttgtatctg ctgcccaagc ttctgccatg aacgcatcca tatctgccgc
    11941 ttcggaatca acgatctgat ttacaactgt ctctacaaat ccctgaaatt catcgttctt
    12001 tgcaacgaag tctgtctcag agtttacttc tacgattgca gctttgttgt cttttacaac
    12061 tgtttttaca ataccttccg ctgcgattct tccagctttt ttctcagctt ttgcctgtcc
    12121 gttctttctg aggaattcta ctgccgcatc catatctccg tttgtttcag agagagcttt
    12181 tttacagtcc atcattcccg ctccggtcat ttctcttaat tcttttacca ttcctgctgt
    12241 gattgccatg ataatttcct ccattatctt ctcaatattc tttttcacct aaacaaatca
    12301 ccgtaagtgc caaacggcgt cttccgggat tgtaagcggt cgccaaggtc tcgggcaggc
    12361 ccggactttt gctcgtcgcc tatacaagca aaaagcttca acctattttt aggctgaagc
    12421 tctcgttttc ttactcttca acagcctctt cagcttctac tgctgtttct gcttcgtagt
    12481 attcttcgtc tgttgctccc tgatttgctt caatcacagc gtcagccatc ttagaaacga
    12541 tcagcttaac agctctgata gcatcatcgt tacccgggat cacataatcc agttcttccg
    12601 gatcgcagtt tgtatcacag ataccgatca acggaattcc aagtgtgtgt gcttcctgta
    12661 cgcagattct ttcttttttc ggatctacga tgaagattgc gtccggcagt ctcttcatat
    12721 ctttgattcc gccaaggttc ttctcaagtt ttgcccactc ttttttcagc tcgataactt
    12781 cttttttcgg aagtacatcg aatgttccgt cttctgacat tgtctcgatc tctttgagac
    12841 gtttgatacg gctctggatc gtcttaaagt ttgtgagcat accgcctagc catctctcgt
    12901 ttacataata cattccacag cgctctgctt ctgttttgat cgcatcctga gcctgtttct
    12961 ttgttcctac gaaaaggatc gtgccgcctt ctgccgcgat gtcagaaacg gctttgtatg
    13021 catcgtcaac cattcctact gatttctgaa ggtcgatgat ataaatacca tttctctctg
    13081 tgtagatgta cggagccatt ttcggattcc atcttcttgt ctgatgtccg aaatgaacac
    13141 ctgcttctaa aagctgtttc attgaaataa cgcccatgtt tctttcctcc aaattggttt
    13201 tttacttccg catgttttgt tttcctccga aaccgtccgg attctccggc ttaggcaccg
    13261 acggagataa ccgcatgcgt gtttttttgc aacattcgta gtatatcata gaagattttc
    13321 ctttgcaagg aattcttcca taagtttttt cacctcttga aggctttttc cttcttcttt
    13381 cgggcgcgcg atcacgataa gttccgcccc ggctttctgc gccgcctttt tcttttcctc
    13441 gaacccgcct gcgccgcctg attctttcgt cacaaaataa gctgcattta tatgtttgag
    13501 cattgccaga ttcagttctt ccgaataggg accctgcatt gcgatcagat gtttcccctc
    13561 aaaaccaaga cggactgcct ctgcgactgc ctcctgcgtc gaaaggactc ttgcaaaaca
    13621 tctttcttta taatctttta tccgggtata caaatgcagt tctttgctcc ctgtagatat
    13681 cagcacattc ccgcctgtct tttttaaata ttccaccgct tcttctaccg aagccgcaga
    13741 tatgggtctt ccgtcactcg tttctcccgc atgatcagcc ggatcgttcg tttctctttc
    13801 ccgaagacag cgaacatatt tgatcttttc ttcttcgcat gcggcgcaaa tattttctgt
    13861 aacttgtctt gcaaacggat gggttgcatc caccgcaagt ccgatattat tttcccttat
    13921 aaacgctctc atctttgact gatccatccg cccgggtatg acccggattc cctcattctc
    13981 tcccagaacg ctttttccat attcggttgc ggtgctgaca caacacttca ttttcatttg
    14041 tgatgcatac cgggtgagca gtctgccttc tgttgttccc gcgaaaatcc atatcttgtc
    14101 ttttttcatc tgactttcac tctttctact cattttttaa cttcggattt ttcttgcctc
    14161 tatcaaaaag ttctcttatc aagcgctttt tcatgcgctt tttaagtctt attttttatt
    14221 tactgccgat attttttacg ttataatcta atacgcggac acgcagaaga aacacttatc
    14281 cttatattta tctataactt ttctataact ttatggaggg attgacctat gatcaaatta
    14341 attgcaagcg acttagacgg cactctgctt caaaacgaag cgcaggagct gacgccccgg
    14401 gcaatcgaac ttatccgggc gctgacccaa aaaggagttc attttgttgc cgcaagcgga
    14461 cgccagtatg caaacgaacg acttctcttt cacgagatca aagacgatat ctcctacatc
    14521 gccgaaaacg gttctctgtg cattcatcag gggaaagtta tctcccgggg actgattgag
    14581 gacgatctcg cttaccgcat tctgcgcgaa ttaaaaaaag aacccggatt tgagattgta
    14641 gtatcacgag aagatacttg cctgatcgaa aacaataact cccaattcgc aaaccacatt
    14701 gtaaatgtga tgcacaacac aaccgagatc gtggacgacc tcttaactgt caaaggtcct
    14761 tttttaaaga ttgcgttcgc gaacatgact tcccgcagaa caacggactg tctgaaagaa
    14821 cttcaggaga aattttcatc cgaaatcaaa gttgtcactt ccggaaacat ttggatcgac
    14881 ttcatcgttc ccgggtgcaa caaaggaagc gcgctccgaa atctgatgga tcttttcggc
    14941 ataacccccg aagaatgtgt cgccttcggc gatcaatata atgatgtgga aatgcttgaa
    15001 acagtcggca tgggatacgc catgtcaaac gccgcccccg gcatcgccgg acatgcaaag
    15061 tatattaccg actctgtaga agaagttctt gaagatattc ttgccggact cgatctttaa
    15121 gaccgacatc cggttttaaa ttctatgacc gcatatggtt ttctgtcttc atcgcaaaga
    15181 cggacgatca cgcggtcttt tttctgtgct gtttccggaa atatcttatc tccaagcact
    15241 gcggacttct tataatcaac ccggacctga ctgactaaga tatccctcgg aagcacttcc
    15301 agcgccatct gaacatactg acagttattt acatgctcat ttgtatctat atggtatttt
    15361 cgcaccggaa acggcgcttt ttcctcataa gactccgctt cttcgatttt gcggtcctca
    15421 tacggcattt ccagcggttc gtcaatccca taggcttccg cctgttcttt gtcaacacga
    15481 accggttttc ctttttctac gtccatatac acccatatcg agttcgcaca ggcaatctgc
    15541 tctccgcttt cgtctttcat ataaaaatta cggtttccga acatcccttt aaaacctgaa
    15601 gcaaatgttc cgactgtgat cctttcgcat agcttcggat accttttcac aatcacctgc
    15661 cagtatgcca aaacccacgc ttttttctct tttttcaaca catccattcc atatccgagt
    15721 tgctcggact gaaaggtact gcagtcctga aaataattga tcaacgccgg aagagtcatt
    15781 gtcgccctgt gatcgatctc actgtatctt accttgcctt caaattcgta tttatgtgct
    15841 ctctccattt ttattccttc ttcctgccat tcttcttgtt g
//
LOCUS       DBMDXF010000019        15445 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1134_length_15445_cov_1.976929, whole genome shotgun sequence.
ACCESSION   DBMDXF010000019 DBMDXF010000000
VERSION     DBMDXF010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15445)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1134_length_15445_cov_1.976929"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            109..588
                     /gene="ybaK"
                     /locus_tag="ACZPD4_01585"
     CDS             109..588
                     /gene="ybaK"
                     /locus_tag="ACZPD4_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1377805.1"
                     /translation="MGKNKEKTNVMRILDQKKIAYESYSYVDTGAVSGIEVAEVLGEN
                     PDMVFKTLVTVGKSKTNYVFVIPVNCELDLKKAAHAVKEKSVEMLKSKELLPLTGYVH
                     GGCSPIGMKKQFTTVIHKTAKNFETMIFSGGKIGYQVELSLEDLLKVIPAKLEDVTE"
     gene            640..1566
                     /locus_tag="ACZPD4_01590"
     CDS             640..1566
                     /locus_tag="ACZPD4_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377806.1"
                     /translation="MMRYTWKRILTGIVSLFLLATVTFFLVRLIPGSPFQRGGVSEQV
                     VEAVEEEYGLNDPLIEQYLSYMGNLLKGDLGISYQDPGTSVEEIIGRAAPLTVSLGLS
                     ALIVSVIVGTGCGVFYAASEKRAVKKVIGSLGMLAAGIPGFAAAILLLMVFSVKLKWF
                     PSSGLFSFSHYILPVTALSLYPTAVITRMTGHALDEEMAKDYVLFARAKGLKKSRIIF
                     THALKNAWFPVLNYIGPASAFLLTGSFAIESVFTIPGLGREFVMSIANRDYTLILGLT
                     VFMGTVVILLNLLTDLLGAWLDPSVRRAYRKK"
     gene            1610..2446
                     /locus_tag="ACZPD4_01595"
     CDS             1610..2446
                     /locus_tag="ACZPD4_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1377807.1"
                     /translation="MRKENRISSKKRKGYRDEKAALIILLIWIFLAVCVPLFGPYSPT
                     EQNAEIRNQPMSLIHFFGTDEFGRDNFTRVWYGAGISLVIGVGSSLINGMIGILYGAV
                     AGYRGKRIDMILMRAADVIAAIPSILYVILITLVMGPGIKSMIFGICIAGWIDMARVV
                     RGEMIRLKETDFAAAAKIDGISGIRILFRHLLPNALGMIVVNFLFLIPQAIFTEAFLS
                     FLGVGLSAPTASLGTLIQEGRDRMILYPCELLAPLITLCVLLVVFHILAMAMERRGGQ
                     TD"
     gene            2449..4221
                     /locus_tag="ACZPD4_01600"
     CDS             2449..4221
                     /locus_tag="ACZPD4_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377808.1"
                     /translation="MLDVRSLYVTYLSEEERVEAVRGIDFSAEEGKVTGIVGESGSGK
                     STAMLAVMGLLDHKAAVEAEKVSLFGNTVRAGDNAAIIFQDPLKCLNPTVKIGRQIAE
                     TVRNRKKCTWREAKKRSEELLDSVGIRHPRLRMKQYPFELSGGMRQRVVIAIALACEP
                     NLIVADEPTTALDAAMQVRVIRLLKKIVKETNTALLLVSHNMGVIAAACDYVYVMKDG
                     KMIENGCADDIFYNPQTEYTKKLLSDATAEQLLVKEKVFAAQENPLFTVRHLKKRFQN
                     EEGVNDVSFDICEGEVFALAGESGSGKTTLAKILTGLEQPDSGEVFCRGERVDRKNRK
                     RNKNMHGKVRMIFQDPYSSLNPCMTINEMLTETLRAKEKQIKFDQKERKKDRQRQIEQ
                     MLTRVGLSSLDGKRYPRELSGGQRQKAAIARALLLEPELLICDEAVSSLDAATRTQIL
                     DLLLRVVKEKKIACLFISHDMALIRRISHRTGVLYKGTLAECAKTRDLCRDPWHPYTK
                     ALLDSVMPPDPLKARKQKVPAVHEGKSEGGCPYFGSCGYKMEMCKDAAPEMYDFEGRA
                     VSCHLYSKQQRAGRNPNYKMTSQI"
     gene            4323..6038
                     /locus_tag="ACZPD4_01605"
     CDS             4323..6038
                     /locus_tag="ACZPD4_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1377809.1"
                     /translation="MRYRKNIKGGEKKIIDQKAILKTVSRGTALFLAAVLFAGSILTG
                     CASTEKSSKSRKNNGSADHEITVAINSETGSLDPAGSIALTYLAYSVSALDELLTFDE
                     NGEIEYRAAQSYEVNEDFTTWTFHLRKDALWSDGGEVTSADFKNTIVRALDPESGSGY
                     AVYLFPILNAEKIYNKEADIETLGVELPDDKTIVFHLEKPCVYFLDLLRLPVYTPSCS
                     KYADAANRGWEKDPKTSLSNGPFYLAEYVPNQYFTLKKNEYYWGKDNISLEKITYRFF
                     DDTQSMAAAYETGEVDVATALPSSVMELYDGKEDLFVTDQIATRYIYFNLNVKPFDDV
                     RVREAFNLAVDREELCKIVGEDTEPTYNLVAKYMKDKNTGKYFTEEAEQPFEENIERA
                     QKLLAEAGYPEGKGFPELTYSYPSLELDSDTAQVIREQLKKNLNIEIKLNAQELQTNY
                     SMRHAGNFDLCRMNWTADFSDPYTYLSMLLSDSTYNCSGIKNEQYDELVRRSDSETDP
                     VKRSALMHEAEQLAVGEQFYILPLYAMKSVNLVNPKIEGIRQIPASGALEYRYAKIRT
                     GHSEK"
     gene            complement(6069..6929)
                     /gene="sdaAA"
                     /locus_tag="ACZPD4_01610"
     CDS             complement(6069..6929)
                     /gene="sdaAA"
                     /locus_tag="ACZPD4_01610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1377810.1"
                     /translation="MDFKSGQQLIGLCETNECPISEIMLIREETFTERTRKDIINQMG
                     KTWQIMKESAHSSVENPKHSIGGLIGGEAKLLSEHLSSGKSICKDPLSRAITYSMSIL
                     ETSASMGLIVAAPTAGSAGIVPGLLLALQETYDFSDNDIIRVLFNAGAVGCLAMRNAT
                     VAGAVGGCQAEVGVASAMAASAAVELMQGTARQCLDAASTVLMNMLGLVCDPVGGLVE
                     CPCQMRNASGVANALTAAEIVLSGVRQLIPFDEMLDAMYTAGKRLPAEYRETALGGCA
                     ATSTGCSLCQ"
     gene            complement(6929..7615)
                     /gene="sdaAB"
                     /locus_tag="ACZPD4_01615"
     CDS             complement(6929..7615)
                     /gene="sdaAB"
                     /locus_tag="ACZPD4_01615"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIR1377811.1"
                     /translation="MSFISVFDVLGPNMIGPSSSHTAGAAAIALLAGKMPNAAIKKAD
                     FILYGSFARTYKGHGTDRALLGGIMGFSTDDRRIPDSFSIADERGLQYSFTANTTETE
                     IHPNTVDIHMTDENGQKLIVRGESVGGGKIRIARINEVEVDFTGEYSTLIVIQQDKPG
                     VIAHITNCLSEMNVNIAYMKLYREEKGCTAYSIVESDGIVPQTVAGRIKENPYVHDVM
                     LVLPNEKGEF"
     gene            7762..8802
                     /locus_tag="ACZPD4_01620"
     CDS             7762..8802
                     /locus_tag="ACZPD4_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1377812.1"
                     /translation="MMELVTGGSGSGKSAYAEDRICALYEEYRKTGKKEQKLYYIATM
                     YPYGTETEEKIADHRRRREGKGFRTLEWYTNITEKIHQFEASGEALGCVLLECVSNLA
                     ANELYMEEGAKDEAVRVVAQAMAMLKKKSCHLVVVTNEIFSESAKDSEEMRKYKYIMG
                     EINKELAKMADEVTEVVCGRPLRRKVKTAEDKTACGNTRRNEGNERQMKTAKCEMSCG
                     KMKRGIRIVTGGAFQGKKNYAQLCYPGLKWSSGADCSFEDVKTWEAVDGFHLFIWRWL
                     KSGRTKEELLEMISEERDLVIVCDEIGCGLVPVDAFEREYREAVGRIMTGLTKKAVRA
                     DRVICGIGYVIK"
     gene            8845..9492
                     /locus_tag="ACZPD4_01625"
     CDS             8845..9492
                     /locus_tag="ACZPD4_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR1377813.1"
                     /translation="MLTEIEKVLPSEIEKRSFEIIAEELGDKQLIPGTESIVKRCIHT
                     SADFDYADNLIFSENVVEKALKAIRGGASIVTDTQMGKAGINKKCLAGYGGEVYCFMS
                     DDDVAQAAKRNKTTRAAASMDKAAKLGEQVIFAIGNAPTALVRLYELIKEEKIAPELV
                     IAVPVGFVNVVQSKELILSLENTPYIVARGRKGGSNIAACICNALLYMGDGGKRE"
     gene            9489..9962
                     /locus_tag="ACZPD4_01630"
     CDS             9489..9962
                     /locus_tag="ACZPD4_01630"
                     /EC_number="1.3.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 dehydrogenase/sirohydrochlorin
                     ferrochelatase family protein"
                     /protein_id="HIR1377814.1"
                     /translation="MKKPYFPMFVDLSGRQVTVVGGGSVALRRSETLLYFGVDIKVIA
                     PEIRSEFYRLDSEGKVVLCNREYQEGDAKGADLVIAASNCREVNRRVYEECQKAHIPV
                     NTADDRELCDFYFPSVVLAEDIVIGLNSIDHDPKKVKEARKIIENVFDNERDYRS"
     gene            10274..10768
                     /gene="nuoE"
                     /locus_tag="ACZPD4_01635"
     CDS             10274..10768
                     /gene="nuoE"
                     /locus_tag="ACZPD4_01635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR1377815.1"
                     /translation="MAEKKATVPFSGTKEQEEALMKVIHELKDEKGALMPILQKAQDI
                     YGYLPIEVQKMISDETGIPMEKIYGVATFYSQFTLSPKGKYRISVCLGTACYVKGSGD
                     IYNALMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMINDEVYGRLTVDDLDDIL
                     AKYE"
     gene            10951..12771
                     /gene="nuoF"
                     /locus_tag="ACZPD4_01640"
     CDS             10951..12771
                     /gene="nuoF"
                     /locus_tag="ACZPD4_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR1377816.1"
                     /translation="MCESGKDADGNTYRTHVLVCGGTGCTSSGSARIRERLEKEIEAN
                     GLSDEVCVVKTGCFGLCALGPIMIVYPEGTFYSMVQEEDIPEIVTEHLLKGNVVKHLL
                     YEETVKADKIIPLNETNFYKKQHRVALRNCGVINPEKIDEYIGTGGYEALGIVLTEKK
                     PEDVIQILLDSGLRGRGGAGFPTGLKWKFAAGNDADQKYVCCNADEGDPGAFMDRSIL
                     EGDPHAVLEAMAIAGYAIGASQGYIYVRAEYPIAVQRLEIAIEQAREYGLLGKNIFDS
                     GFDFDIELRLGAGAFVCGEETALMTSIEGNRGEPRPRPPFPALKGLFQKPTILNNVET
                     YANIPQIIVNGPEWFASMGTEKSKGTKVFALGGKIHNTGLVEIPMGTTLREIVEEIGG
                     GVPNGKKFKAAQTGGPSGGCIPAEHLDIPIDYDNLLSIGSMMGSGGLIVMDEDTCMVD
                     IAKFFLEFTVDESCGKCTPCRIGTRRMLEILEKITKGQATMEDLDKLEELCYHLQSNS
                     LCALGQTAPNPVLSTLRYFRDEYIAHIVDKKCPAGVCKDLLQYKIDPDKCKGCTLCAR
                     TCPADAIIGKVKEVHMINPEKCLKCGACMEKCRFGAIYKE"
     gene            12786..14528
                     /locus_tag="ACZPD4_01645"
     CDS             12786..14528
                     /locus_tag="ACZPD4_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HIR1377817.1"
                     /translation="MGNVNLKINGLNVTAPAGSTILEAAQAAGIKIPTLCYLKEINEI
                     GACRMCVVEVKGARNLVASCVHPVAEGMEVQTNTPKLLDSRRRTLELLLSNHDKKCLS
                     CVRSGQCELQELCQELGVTDENYFEGETTHYELDESAVHMVRDNNKCILCRRCSAVCE
                     KVQSVGVIGPNNRGFATFIGSPFEMGLGDTSCVGCGQCIAACPTGALYEKSNIDDVLA
                     AIADETKHVVVQPAPSVRAALGEEFGYPMGTDVEGKMAAALRRIGFDKVFDTNFSADL
                     TIMEEAHEFLDRVKNKGVLPLMTSCSPGWVKYCEHYYPDQLDHLSSCKSPQQMFGAIT
                     KTYYAEKMNIAPEDIVCVSVMPCTAKKFEIQREDQDAGGVPDVDISITTRELARLIRK
                     VGINFRSLPDEGFDDPLGESTGAGVIFGATGGVMEAALRTAVEELTGETLEKVEFTEV
                     RGTEGIKEAVYNVAGMDVKVAVASGLSNAKIIMDKIRAKEADYHFVEIMCCPGGCVNG
                     GGQPQVHADVRNFEDVKAIRAKVLYDNDAAKTLRKSHENPSIKRVYSEYLGEPGSEKA
                     HHILHTTYVKRTVN"
     gene            14744..>15445
                     /locus_tag="ACZPD4_01650"
     CDS             14744..>15445
                     /locus_tag="ACZPD4_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1377818.1"
                     /translation="MGWDWHKFRKDTAFATRNLVWYTLIMSVVTEMVSAMVSALTKTG
                     GSSESLISEVTGTAASAGMIAGVLIGVFCLFKIDGTKAEKIDIFQKSKRKMTAGAFSR
                     FCIYMIMAQMVFSLIAAAAELILNQLGLSMDTLTELASVGSDMGIMMMIYAGFVGPVV
                     EELVFRGFLMRRFEKYGKGFAVIVSAVLFGVMHGNPLQIVFGTLVGLILGYIAIEYSI
                     KWSIILHIANNFILGD"
BASE COUNT         4655 a         2724 c         4164 g         3902 t
ORIGIN      
        1 tgaaaattat ggaaagaagg aataattatg tcgtttttaa acagtagaaa agtatcatgg
       61 attatcacag aagcatttcg gtatagatag aattcggaag gaagaataat gggaaaaaat
      121 aaagaaaaaa caaatgtaat gaggatttta gatcagaaga agattgcata tgaaagctat
      181 agttatgttg atacgggagc ggtaagcgga attgaagtag cggaagtttt gggtgaaaat
      241 cctgatatgg tgtttaaaac tcttgtgacg gtcggaaaat caaaaacgaa ttatgtcttt
      301 gtcattccgg ttaactgtga actggatcta aagaaagcgg cacatgccgt aaaagaaaaa
      361 agtgttgaaa tgctaaaatc aaaagaactg cttcccctta ccggatatgt tcacggcgga
      421 tgttctccga ttggaatgaa aaaacagttt acgacagtga tacataaaac ggcaaaaaac
      481 tttgaaacga tgattttcag cggcggcaag atcggatatc aggtagaact tagtctggaa
      541 gatcttctga aagtcattcc ggcaaagctg gaggatgtga cggaataaaa cggcagagca
      601 aagaaaaaaa cagggtctga acaaggaggt gaaagggcaa tgatgcgtta cacatggaaa
      661 agaatattga ctgggatagt atcattgttc cttcttgcca cggtcacttt ttttctcgta
      721 aggctgatac cgggaagtcc gtttcaaaga ggcggcgttt cggagcaggt tgtggaagct
      781 gtggaagaag aatatgggtt gaacgatccg ctgatcgagc agtatctgtc atatatggga
      841 aatcttttaa aaggagatct ggggatttct tatcaggatc cgggtacatc ggtggaagag
      901 attatcggac gggcagctcc gcttacggtg tcgctcggac tttcggcatt gatcgtatca
      961 gtgatcgtcg gtacgggatg cggggtgttc tatgccgcgt cggaaaaaag ggcagtgaaa
     1021 aaggtaatcg gatcattagg aatgctggcg gcgggcattc ccggttttgc cgctgcgatt
     1081 cttctgttaa tggtattcag tgtaaaatta aaatggtttc cttcgtcggg tttattttct
     1141 ttttcacatt atattttacc ggttacggca ttgtcgcttt atccgacggc tgtgatcacc
     1201 cgaatgacag gacatgcatt ggatgaggag atggcaaaag attacgttct ttttgccagg
     1261 gcaaaaggat tgaaaaagag caggatcatt tttactcatg cgttaaaaaa cgcatggttt
     1321 ccggtattga attatatcgg tccggcatcg gcgtttctgc ttacgggaag ttttgccata
     1381 gagagcgtgt ttacgattcc gggtctggga agagagtttg tcatgtcgat tgcaaacaga
     1441 gattatactc ttattctcgg attgactgta tttatgggaa cagttgttat tcttcttaat
     1501 ttgctcacag atctgcttgg ggcatggctt gatccaagtg tcagaagagc ttacagaaag
     1561 aaataagaaa aaacttgtgt caggaaccgg cgggaggtgt gacaaaaata tgcggaaaga
     1621 aaacagaata agcagtaaaa aaagaaaggg atatagggat gaaaaagcgg cgcttatcat
     1681 ccttttgatc tggatctttc tggcagtatg cgtaccttta ttcgggcctt attctccgac
     1741 ggaacaaaat gcggaaatac ggaaccagcc aatgtcgttg attcattttt tcggtacaga
     1801 cgagtttgga agagataatt ttacacgggt ttggtatgga gcaggcatta gtcttgtgat
     1861 cggcgtggga agttctctga tcaatgggat gatcggtatt ttgtacggtg ctgtagcggg
     1921 gtatcgtggg aaacgcatcg atatgattct gatgagagca gcggatgtga ttgccgcgat
     1981 cccgtcgatt ttatatgtta tactgataac gctcgttatg ggaccgggga taaaaagcat
     2041 gatcttcggg atttgtattg ccggctggat cgatatggcg cgtgtcgtac ggggggagat
     2101 gatccgttta aaagaaacag attttgcggc ggcggcaaag atagatggta tatcgggcat
     2161 tcggattttg tttagacatc tgcttccgaa cgcactgggg atgatagtcg taaacttctt
     2221 gtttctgata ccgcaggcga tttttacaga agcattttta agctttttgg gggtgggatt
     2281 atctgcgccg acggcaagtc tcggaacatt gatacaggaa ggacgagaca ggatgattct
     2341 ttatccgtgt gaacttttgg cgcctcttat tacgttgtgt gtgcttttgg tcgtgtttca
     2401 tatacttgca atggcgatgg aaagaagagg agggcagaca gattgaatat gctggatgta
     2461 agatcacttt atgtaacgta tttgtctgag gaagaacgcg ttgaagctgt acgggggata
     2521 gatttttcgg cggaagaagg aaaagtgacg ggaatcgtag gcgaatccgg ttcaggcaag
     2581 agtacggcaa tgctggctgt gatgggactt ttggatcata aagccgctgt agaggcagag
     2641 aaagtgtctc tttttggaaa tactgtcaga gcaggagaca atgcggccat tatttttcaa
     2701 gatcctttaa aatgtctgaa tccgacagtg aagatcggca gacagattgc cgagactgtg
     2761 cggaatagga aaaaatgtac atggcgggag gcaaagaaaa gatcggaaga actattggat
     2821 tcggttggta tccgccaccc caggttaagg atgaaacaat atccttttga actttccgga
     2881 ggaatgagac aaagagttgt tattgcgatt gcccttgcct gcgaaccgaa tctgatcgtg
     2941 gcagacgaac ctacgacggc gcttgacgcg gcgatgcagg tcagagtaat ccggctgttg
     3001 aaaaaaatag taaaagaaac aaatacagct cttcttcttg taagtcataa tatgggggtg
     3061 atcgctgcag cttgtgatta cgtttatgtt atgaaagacg gaaaaatgat agagaacgga
     3121 tgtgcagatg atattttcta caatccacag actgaatata caaagaaact tctgtctgat
     3181 gcaacggcag aacagcttct tgtaaaagaa aaagtgtttg ctgctcagga aaatccgctg
     3241 tttacggtaa gacatttgaa aaagcggttt caaaacgaag aaggggtgaa cgatgtgtcg
     3301 tttgatattt gtgagggtga agtgtttgct cttgcaggag aaagcggcag tggaaaaacg
     3361 acgctggcaa aaatacttac agggctggaa cagcctgaca gtggggaagt gttttgcagg
     3421 ggagaaagag tagatcgaaa aaacagaaaa agaaacaaaa atatgcatgg gaaagtcagg
     3481 atgatatttc aagatccgta ttcatccctg aatccgtgca tgacgataaa cgaaatgttg
     3541 acggaaacgc ttcgggcgaa agaaaaacaa ataaagtttg atcaaaagga aaggaaaaaa
     3601 gaccggcaaa gacagataga acagatgctt acgcgcgtag gattgagtag tttagacggg
     3661 aaacggtatc caagagaact ttcaggcggg cagcgtcaga aagcggcaat cgccagggcg
     3721 ctccttttag agccggaact tctcatttgt gatgaagctg tttcttcatt agatgcagcg
     3781 acaagaacgc agatattgga tctgctgctg cgagtagtaa aagaaaaaaa gatcgcctgt
     3841 ttgtttattt cgcatgacat ggcgctgatt cgccggatca gtcacaggac aggcgttctc
     3901 tacaaaggaa cactggcgga atgcgcaaaa acccgggatt tatgcaggga tccgtggcat
     3961 ccgtatacga aagcacttct cgattcggta atgccgcctg atccgttgaa ggcaagaaaa
     4021 caaaaagtac ctgcagtgca tgaaggaaaa tcagaaggag gctgtccgta tttcggatct
     4081 tgcggatata agatggaaat gtgcaaagac gcagcccctg aaatgtatga ttttgaaggc
     4141 agagcagttt cctgtcacct ttattccaaa caacaacgcg ccggaagaaa tccgaattat
     4201 aagatgacat ctcagatata aaaaagcaaa attacgaaaa ctacagatat agaaacggac
     4261 atcggtatag atgaattgtt gcacaaagaa tcgggaatca tgaaaatagg agataaataa
     4321 aagtgagata cagaaaaaat ataaaaggcg gggaaaaaaa gattatagat cagaaggcca
     4381 tcctgaaaac agtgtcgaga ggaacagctt tgtttttggc ggcggtattg tttgcgggaa
     4441 gtatcctgac agggtgtgca agtacagaaa aatcgtcaaa aagcaggaaa aacaacggca
     4501 gtgccgatca tgagattaca gttgcgatca acagtgaaac cggatcgtta gatccggcag
     4561 gatcaatcgc tttgacgtat cttgcatatt cggtaagcgc tttagacgaa ctgctgacct
     4621 ttgatgaaaa cggagaaatc gagtaccgtg cggcacagtc ttacgaagta aatgaagatt
     4681 ttacaacgtg gacgtttcat ttgcgaaaag atgcgctttg gtctgacgga ggagaagtga
     4741 catcggcaga ttttaagaat acgatcgtaa gagcgttaga ccctgaatcg ggaagcggat
     4801 atgcagttta tctttttccg attttaaatg cagaaaaaat ttataataaa gaggcggata
     4861 tagagacgct gggagttgag cttcccgatg ataagacgat tgtttttcat cttgaaaagc
     4921 cgtgcgtata ttttcttgat cttctccggc tcccggtata tacgccttcc tgctcgaaat
     4981 atgcggatgc tgctaaccgt ggctgggaga aagatccgaa gacgagttta tcaaacggtc
     5041 ctttttattt ggctgaatat gtaccgaatc agtattttac gctgaaaaaa aatgaatatt
     5101 actggggaaa ggacaatatc agcttagaga agatcaccta tcgttttttt gatgacacac
     5161 agtctatggc agcggcttat gaaacgggag aagttgatgt ggcaacagcg cttccttctt
     5221 cggtcatgga attatatgac ggaaaagaag atctgtttgt gacagatcag attgcgacac
     5281 gatatattta ttttaatctg aatgtgaagc cgtttgacga tgtccgtgtg agggaggcgt
     5341 ttaatcttgc ggttgacaga gaagagttgt gcaagattgt cggagaagac acagagccga
     5401 cgtataatct ggtggcaaaa tatatgaaag acaaaaatac ggggaaatat tttacggagg
     5461 aggcagaaca gccttttgaa gaaaatatag aacgggcaca gaaacttttg gcagaagccg
     5521 gatatcccga aggaaaagga tttcctgaac tgacatatag ctatccgtct ttggaacttg
     5581 actcggacac ggcgcaggtt atccgggaac agttgaagaa aaacctcaat atagagatca
     5641 agttaaacgc tcaagaatta cagacaaatt acagtatgcg tcatgccggt aattttgatc
     5701 tgtgccggat gaactggacg gcggactttt ctgatccata cacatatctt tcgatgcttc
     5761 tgtcggatag tacgtataat tgcagtggga tcaaaaatga acagtatgat gaacttgtcc
     5821 gaagatcgga ttccgaaaca gatcctgtaa aacggtctgc gctgatgcat gaagcggaac
     5881 aactggcagt cggagaacag ttttatatac ttccgcttta cgcgatgaag agcgttaatc
     5941 ttgtgaaccc gaagatcgaa ggaattcgtc agattccggc gagcggcgct ttggaatatc
     6001 gatatgcgaa aatcaggacc ggtcattcgg agaaataacc ggtctgcacg ctgcaaaata
     6061 ttttctgctc actgacagag ggaacatccc gtagaagtag ctgcacagcc gccgagagcg
     6121 gtttcgcggt attcggcagg aagtctcttt cctgcggtat acatagcgtc taacatttcg
     6181 tcaaagggga tgagttgccg gactccgctt aaaacaattt cggctgcggt taatgcgttg
     6241 gcgacacccg atgcgttgcg catctgacag gggcattcca caagaccgcc gacagggtcg
     6301 catacaagtc cgagcatatt catcagtacg gtggacgcgg cgtcaaggca ttgccgcgca
     6361 gtaccctgca tcagttcgac tgcggcagaa gcggccattg ccgaggcgac tccgacctct
     6421 gcctgacagc cgcccacagc gccggcaaca gtagcgttgc gcatggcaag acagccgacg
     6481 gcccctgcat taaaaaggac acggataata tcgttgtcag agaaatcata tgtctcctga
     6541 agcgcaagaa gcagtccggg aacgattccc gcagacccgg ctgtaggcgc ggcaacgatc
     6601 agtcccatag aggcgcttgt ttcgaggatt gacatggagt aagtaattgc ccggcttaat
     6661 gggtctttac agatgctttt cccggaactg agatgttcac ttagaagttt tgcttcgcct
     6721 ccgatgagtc cgcctatgga atgttttgga ttttcaacag aggaatgggc agattctttc
     6781 atgatctgcc atgtttttcc catctggttt ataatgtcct ttcgtgttct ttccgtaaaa
     6841 gtttcttccc gtatcagcat gatctctgaa ataggacatt cgtttgtttc acataatccg
     6901 atcagctgct gtccgctttt aaaatccatt aaaattctcc tttctcattc ggaagtacga
     6961 gcatgacgtc atgtacataa ggattttctt tgattcgtcc ggctactgtc tgaggaacga
     7021 ttccgtcaga ttcgacgatg ctgtacgccg tacacccttt ttcttcccgg taaagcttca
     7081 tataagctat attgacgttc atttcgctta agcagtttgt gatgtgtgcg atcactccgg
     7141 gtttgtcctg ttgaatgacg ataagagtgc tgtattctcc ggtaaaatca acttccactt
     7201 cgttgattct tgcgattcgg atttttcctc ctccgaccga ttctcctcga acgatgagtt
     7261 tttgtccgtt ttcgtcagtc atatggatgt ctactgtgtt agggtgaatt tcggtttctg
     7321 ttgtatttgc cgtaaacgaa tactgaagtc cgcgctcatc ggcgatagaa aatgaatccg
     7381 gaatccgtct gtcgtcggtt gaaaatccca tgatccctcc gagcagggcg cggtctgttc
     7441 cgtggccttt atatgtgcgg gcaaaagacc cgtaaagaat aaaatcagct tttttaatgg
     7501 cggcgttcgg catctttcct gcaagaaggg cgattgccgc agcgccggcc gtgtgcgaac
     7561 tggacggacc gatcatgttc ggaccgagca catcgaatac actgataaat gacataaaag
     7621 ttcctcctgt tctttttgtt gacgctcata tagaggtagt atatcatgtt ttcaaataag
     7681 aatgtgggtt cagagaagaa aagatgcgtt tattgtgtat agaaaagcta tcgggaaaga
     7741 aaaaagaaag gttttaaaaa tatgatggag ttagtaacag gaggaagcgg aagcgggaaa
     7801 tccgcgtatg cagaagacag gatctgcgca ttgtatgaag aataccgtaa gacaggaaag
     7861 aaggaacaaa agctgtatta tatagcgacg atgtatccgt atggaacgga aacagaagag
     7921 aaaatagccg accacaggag acgaagagaa ggaaaagggt ttcggactct tgaatggtat
     7981 acaaacatta cggaaaagat ccatcagttt gaagcgtccg gggaggcgct tggatgtgtt
     8041 cttttagagt gcgtctctaa tttggctgcg aacgaattat atatggaaga aggggcgaaa
     8101 gacgaggctg tccgggttgt tgcgcaagcc atggcaatgt tgaaaaagaa aagttgtcat
     8161 cttgttgttg tgacaaatga aatcttttcg gaatcggcaa aagattcgga agagatgaga
     8221 aagtataagt atattatggg agagatcaac aaagagcttg cgaagatggc agatgaagtg
     8281 actgaagtcg tctgcggaag acctctcaga agaaaagtaa agaccgcgga agataaaact
     8341 gcatgcggga atacaagacg gaatgaaggg aacgaaagac agatgaagac ggcaaagtgt
     8401 gaaatgtcct gcggcaaaat gaaaagagga atcaggatcg tgacaggggg agcgtttcag
     8461 ggaaagaaaa actacgcgca gttatgctat ccgggactga aatggagcag cggagcggac
     8521 tgtagtttcg aggatgtcaa aacatgggag gctgtagatg ggtttcatct ttttatatgg
     8581 cgctggctga aatcaggaag aacaaaagaa gaactgttag aaatgataag cgaagagaga
     8641 gatcttgtta tcgtgtgtga cgagatcggg tgcggtctgg ttccggttga tgcgtttgag
     8701 cgtgagtaca gagaggcagt cggaagaatt atgaccggtt tgactaaaaa ggcagttcgg
     8761 gcagaccgcg tgatctgtgg gatcggatat gttataaaat gaaaagaaac actgaaaacg
     8821 atcaaagaaa aggaggaaaa gaagatgttg acagagatag aaaaggtgct tccgtcggag
     8881 attgagaaaa gaagttttga gatcattgcg gaagaactgg gggataagca gttgattccg
     8941 ggaacggaat cgatcgtgaa aagatgtatc catacaagcg ctgattttga ttatgcggat
     9001 aatcttattt tttcggaaaa tgtagttgaa aaggcgctaa aagcaatccg cggcggagcg
     9061 tctattgtga cagatacaca aatgggaaaa gcggggatca ataaaaaatg tcttgccggc
     9121 tatggtggag aggtatattg ctttatgtcg gatgacgatg tggcgcaggc ggcaaaaaga
     9181 aacaaaacaa cacgggcggc cgcgagtatg gataaggcgg caaaattagg ggagcaggta
     9241 atctttgcca tcggaaatgc tccgacagcg cttgtgcgtt tatatgagct gatcaaagag
     9301 gaaaagatcg ccccggaact tgtgatcgcg gttccggtcg gatttgtcaa tgttgtacag
     9361 tcaaaagaat tgatcttaag tcttgaaaat actccgtata ttgttgcgag aggacgaaaa
     9421 ggcggcagca atattgcggc atgtatatgt aatgcacttt tgtacatggg agacggagga
     9481 aaaagagaat gaaaaagccg tattttccga tgtttgttga tctttcgggc agacaggtta
     9541 ctgtagtggg agggggcagt gttgcgcttc gccgatcgga gacgctgctt tatttcggcg
     9601 tggatattaa agtgatcgcg ccggagatca gaagtgagtt ttatcgtctg gattccgaag
     9661 gaaaagttgt tttgtgtaac agagaatatc aggaagggga tgcaaaagga gcagatcttg
     9721 tgatcgccgc atcaaactgt cgggaagtaa accgccgtgt gtatgaagag tgtcaaaaag
     9781 cacatattcc ggtcaacacg gcagacgaca gagagttgtg tgacttttat tttccgtctg
     9841 tcgtattggc agaagatatt gtcatcggac taaattccat agatcacgat ccgaagaaag
     9901 tgaaagaagc gagaaagatt attgaaaatg tgtttgataa tgaacgagat taccgttcct
     9961 gataataaga taaagaaggt gttaaaacat tctgtcagca gaaaaactgc tggcagtttc
    10021 tgcattggcg acagactgaa gtgtggatcc gttcgatccg tcgatgtttc ggtaataaat
    10081 gtaaaaagat aaaagatata aaaaatgaaa aagaaacaga tgtattatgt gtaaaatgta
    10141 aaaacaaaag cggtgcacat tgtttttggt gataaaaata gacaaaaaat taacaaaaaa
    10201 taagtagaat tatgatattt tgtatgatat gatatctcta atgggcatta acagtaagaa
    10261 tggaggttga agaatggcag agaaaaaggc gactgttcct ttctcgggaa caaaagaaca
    10321 ggaagaagca ctcatgaaag taatccatga gctaaaagat gaaaaagggg cgctgatgcc
    10381 gatcttgcag aaagcgcagg atatatatgg gtatcttccg atcgaagttc agaaaatgat
    10441 ctcagacgag acaggtattc cgatggagaa gatctatgga gtggcaacat tttattcaca
    10501 gttcacattg agtccgaaag gaaaatacag gatttccgtc tgtcttggaa cggcatgtta
    10561 tgtaaaagga tcgggagata tttacaatgc tttgatggaa aagttaggta ttgtcggcgg
    10621 agaatgtacg ccggacggaa agttttctct tgatgcgtgc cgatgtgtcg gagcatgcgg
    10681 attagctcct gttatgatga tcaatgacga agtatacgga cgtttgacag tagatgatct
    10741 ggatgacatt ttagcgaagt atgaataatg cggacatcaa aatcccggat aatatgaaaa
    10801 cgggaatatg atacttattg attttagaaa aacaggaaat ggaggagtta tatgaaatct
    10861 cttgaacaat tacatgagat ctgtgagcgg atgcgtaaag tcgtgcagct tagagaagag
    10921 aatgcgaaag aaatacttga gaaagccgca atgtgtgaaa gcggaaaaga tgccgatgga
    10981 aatacatacc gcacgcatgt gcttgtctgc ggcggaaccg gatgtacgtc ttccggaagc
    11041 gcccggatca gagagagact ggaaaaagag atcgaagcga acggattgtc cgatgaagtc
    11101 tgtgttgtta agacaggttg tttcggcttg tgtgcgttag gcccgatcat gatcgtatat
    11161 ccggaaggaa cgttttattc tatggttcag gaagaagata ttccggagat tgtgacagaa
    11221 catctgctca aaggaaacgt ggtaaagcat ctgctttatg aagagactgt aaaagcagat
    11281 aagatcattc cgttaaacga gacgaatttt tataaaaaac agcacagagt cgctctgcga
    11341 aactgcggtg tcatcaatcc cgaaaagatc gacgaatata tcggaacagg cggatacgaa
    11401 gcgctcggta ttgttctgac ggaaaagaag ccggaagatg tgattcagat tcttttggac
    11461 agcgggcttc gcggcagagg cggagcagga ttcccgacag ggttaaaatg gaagtttgcg
    11521 gcaggaaatg acgcagatca aaaatatgtc tgttgtaatg cggacgaagg agatccggga
    11581 gcatttatgg atcgttcgat cttagaggga gatcctcatg cggttcttga agcaatggcg
    11641 atcgccggat atgcgatcgg agcatcccaa ggctatattt atgttcgtgc agagtatccg
    11701 atcgccgtgc agcgtcttga gattgcgatc gaacaggcaa gagagtacgg tctgctcgga
    11761 aaaaatattt tcgatagcgg atttgacttt gatattgaat taaggcttgg agcaggcgcg
    11821 tttgtctgcg gagaagagac ggctcttatg acttctatag aaggaaacag aggagagccg
    11881 cgtccgcgtc cgccgttccc ggcgctgaaa ggcctgtttc aaaaaccgac aattttaaat
    11941 aacgtggaga cgtatgccaa tatccctcag attattgtga acggaccgga gtggttcgct
    12001 tcgatgggaa cggaaaaatc aaaaggaacg aaagtatttg ctctcggcgg aaagatccat
    12061 aatacaggac ttgtagaaat tccgatggga acgacgcttc gcgagatcgt agaagagatc
    12121 ggcggaggag tgccaaacgg aaagaaattc aaagctgctc agacgggagg cccgtcagga
    12181 ggatgtatcc ctgcagaaca tttagatatt ccgatcgatt acgataatct gctttcaatc
    12241 ggttcaatga tgggttccgg cggtttgatc gtcatggatg aagatacttg catggtcgat
    12301 atcgcgaaat tcttccttga gtttacggta gatgagtcat gcggaaaatg tactccgtgc
    12361 cggatcggaa cacgccgtat gcttgaaatc cttgagaaga tcacaaaagg tcaggcaacg
    12421 atggaagatc tcgataagct ggaagaactg tgctatcatc tccaatcaaa ttctctgtgt
    12481 gcgctcggac agacggctcc gaatccggtg ctttcgactt tgagatattt cagagacgag
    12541 tatattgctc acattgtaga taaaaaatgc cctgccggag tatgtaaaga tctccttcaa
    12601 tataagatcg atccggataa atgtaaaggt tgtacgctct gtgcgagaac ctgtccggca
    12661 gatgcgatca tcggtaaagt aaaagaagtg catatgatca atcctgaaaa atgtctcaag
    12721 tgcggagctt gtatggaaaa atgccgcttc ggagcaattt acaaagaata aggagggcag
    12781 agaagatggg aaatgtgaat ttaaagatca atggtttaaa tgtgacagcg cctgccggct
    12841 ctacgatcct tgaagcggca caggctgcgg gaattaagat accgactttg tgttatttaa
    12901 aagagatcaa tgagatcggc gcctgccgta tgtgcgtcgt ggaagtaaaa ggcgcaagaa
    12961 atctcgtggc atcttgtgta catccggttg cagaagggat ggaagtgcag acgaatacgc
    13021 cgaaacttct tgattcgaga agaagaacat tagagctgct tttgtcgaat catgacaaaa
    13081 aatgtctgtc ttgtgtcaga agcgggcagt gtgaacttca agagctttgt caggaactcg
    13141 gagtgacaga tgaaaattac ttcgagggcg agacaacaca ttatgaattg gatgaaagcg
    13201 cggttcatat ggtgcgggat aacaacaaat gtatcctttg ccggagatgt tctgccgtct
    13261 gtgaaaaagt gcagagcgta ggagtgatcg gaccgaacaa cagaggattt gccacgttta
    13321 tcggctctcc gttcgagatg ggactgggag atacaagctg cgtcggctgc ggacagtgta
    13381 tcgcggcatg tccgacgggc gcgctctacg aaaagagcaa tatcgacgac gttcttgccg
    13441 ctattgcgga tgagacaaaa catgttgtag tacagcctgc tccgtcggtg cgggcggctc
    13501 ttggcgaaga attcggttat ccgatgggaa cagacgtaga aggaaagatg gctgcggctc
    13561 ttagaaggat cggatttgat aaagtattcg atacaaattt cagtgcagac cttacgatta
    13621 tggaagaagc tcatgaattt ttagacagag tgaaaaacaa aggagtcctt ccgcttatga
    13681 cgtcatgctc acccggatgg gtaaaatatt gcgagcatta ctatccggat cagctcgatc
    13741 atctttcaag ctgcaaatca ccgcagcaga tgttcggagc gattacgaaa acatattatg
    13801 cggaaaagat gaacattgcg cctgaagata ttgtctgcgt cagcgtgatg ccgtgtacgg
    13861 cgaagaagtt tgaaatccaa agagaagatc aggacgcagg cggcgttcct gatgtagata
    13921 tttcgatcac gacaagggaa cttgcacgcc tgatccgtaa agtcggaatt aatttccgca
    13981 gtcttccgga tgaaggattc gacgatccgc tcggagaatc tacaggagcg ggagtgatct
    14041 tcggcgcaac aggaggcgtt atggaagcgg ctcttcgtac cgcagtggaa gaactgacag
    14101 gcgagacgct tgagaaagta gaatttacag aagttcgtgg aacagaggga atcaaagaag
    14161 cggtttacaa cgtagcagga atggatgtga aagtcgccgt tgcttccgga ctttccaatg
    14221 cgaagattat catggataaa ataagagcta aagaggcgga ctatcatttt gttgagatca
    14281 tgtgctgtcc tggaggatgt gtcaacggcg gcggtcagcc tcaggtgcat gcggatgtgc
    14341 gtaattttga agatgtaaag gcaattcgtg caaaggtact ttatgataac gatgccgcaa
    14401 aaacattgag aaaatctcat gagaatccgt ccatcaaacg ggtttacagc gaatatctcg
    14461 gcgaaccggg aagtgagaaa gcgcatcata ttcttcatac aacatatgta aaacgtacag
    14521 tgaattaaaa agtccgagaa acaactgatc ttagttgtta tggaccgcgc tgttactata
    14581 tgtcaaatat tttataacac agggaagtct tttagatttt cctgtgttat aattataaaa
    14641 agctgtttta ttaaaaagat aactgacagg aaatgaagat aaaacaaaaa gttgtgaaca
    14701 atgtgacttt gccggtcggt ttatcgggaa aggggaacgg aatatgggat gggattggca
    14761 taagtttcgg aaagatacgg catttgcgac aagaaatctg gtctggtaca ctttgatcat
    14821 gtcggttgtg acagagatgg tctccgcaat ggtttcggca ttgacgaaga caggcggttc
    14881 gtcagagtcg cttatttcgg aggtgacggg aacggcagct tccgcaggga tgatcgcagg
    14941 agttttgatc ggagttttct gtctttttaa gattgacggt acaaaagcag aaaagatcga
    15001 tattttccaa aaatcgaaac gaaagatgac agcaggcgca ttttcaagat tttgcatcta
    15061 catgatcatg gctcagatgg tcttttcgtt gatcgcagca gcagcggaac tgattttgaa
    15121 tcagttgggg ctttcgatgg atacgctgac agagcttgct tcggtcggtt ctgatatggg
    15181 gattatgatg atgatctatg ccggattcgt cggaccggtt gtggaagaac tcgttttcag
    15241 aggatttctt atgagacggt ttgaaaaata tggtaaagga tttgcggtta ttgtctcggc
    15301 agttttgttc ggtgttatgc acgggaatcc gcttcagatc gtattcggaa cgctcgtggg
    15361 acttatttta ggatatatag caatcgaata ttcgataaaa tggtcgatca tccttcacat
    15421 cgcgaataac tttattctcg gagat
//
LOCUS       DBMDXF010000020        15381 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1137_length_15381_cov_2.023560, whole genome shotgun sequence.
ACCESSION   DBMDXF010000020 DBMDXF010000000
VERSION     DBMDXF010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15381)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_15381_cov_2.023560"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(461..550)
                     /locus_tag="ACZPD4_01655"
     tRNA            complement(461..550)
                     /locus_tag="ACZPD4_01655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(513..515),aa:Ser,seq:gga)
     gene            complement(556..641)
                     /locus_tag="ACZPD4_01660"
     tRNA            complement(556..641)
                     /locus_tag="ACZPD4_01660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Ser,seq:tga)
     gene            complement(916..1680)
                     /gene="ispD"
                     /locus_tag="ACZPD4_01665"
     CDS             complement(916..1680)
                     /gene="ispD"
                     /locus_tag="ACZPD4_01665"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR1377819.1"
                     /translation="MLKPKNVIDREDKGEIVEAEGGGTMTKVYCTAIVLAAGSGKRMG
                     TKVHKQYLQLCEKPVLAYSLQAFQQSDLIDEIILVVGKGEEEFCRTEIVEKYGFFKVQ
                     KIICGGEQRYHSVWNGLKETKAGYVYIHDGARPFVNENIIRRGYECVVREHACAAGMP
                     VKDTVKIVDDNNIVSQTPERKYVWIVQTPQVFDTELIKRAYRLLEKEGESAVTDDAMV
                     VETMLGESVKLYEGSYENIKITTPEDLKVAEAFVGK"
     gene            complement(1887..2915)
                     /gene="tsaD"
                     /locus_tag="ACZPD4_01670"
     CDS             complement(1887..2915)
                     /gene="tsaD"
                     /locus_tag="ACZPD4_01670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1377820.1"
                     /translation="MKEIRNINILAIESSCDETAAAVVHNGRKVLSNIISSQIDLHKL
                     YGGVVPEIASRKHIEKINQVIEEALKEAEVTLKDIDAVAVTYGPGLVGALLVGVAEAK
                     AIAYAAGLPLVGVHHIEGHISANYIEHPELEPPFLCLVASGGHTHLVCVKDYGKYEIL
                     GRTRDDAAGEAYDKVARAIGLGYPGGPKIDRIAREGNPDAIKFPKANVDGAKYDFSFS
                     GLKSAVLNYINGCKMKGESFDPADIAASFQKAVVEVLVEKSMQAAEDYSMRKFAIAGG
                     VASNTALRTAMEAACAKRGIQFYHPSPIYCTDNAAMIGAAGFYEYLAGTRHGWDLNAV
                     PNLKLGER"
     gene            complement(2926..3834)
                     /locus_tag="ACZPD4_01675"
     CDS             complement(2926..3834)
                     /locus_tag="ACZPD4_01675"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR1377821.1"
                     /translation="MLDVCLLGCGGMMPLPYRYLTALMTRFNGSSMLIDCGEGTQVAI
                     KEKGWSFKPIDVICFTHYHGDHISGLPGLLLTMGNADRTEPLTLIGPKGLERVVSCLR
                     VIAPELPFPIVFKEIEGAEQTFEINGYRLKAFRVNHNVLCYGYTMEIDRAGKFDAQRA
                     KEQGIPLPYWSRLQNGETIETEDKVYTPDLVLGPPRKGIKLTYTTDTRPTDSIKENAA
                     GSDLFICEGMYGEQEKAAKAVEYKHMTFYEAAALAKEAEVEEMWLTHYSPSLTKPQYY
                     MDEVRRIFPRAKAGNDGMTAELMFED"
     gene            complement(3869..4108)
                     /locus_tag="ACZPD4_01680"
     CDS             complement(3869..4108)
                     /locus_tag="ACZPD4_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377822.1"
                     /translation="MRRYYLKEREMIKETEAIICNRCGKEIVVRNGEPREGIFSADCE
                     WGYFSEKDGERHHFDLCESCYDDLVSSFKIPVDAE"
     gene            complement(4228..5412)
                     /gene="tsaB"
                     /locus_tag="ACZPD4_01685"
     CDS             complement(4228..5412)
                     /gene="tsaB"
                     /locus_tag="ACZPD4_01685"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1377823.1"
                     /translation="MRVLAIDSSGLTATVAIVEDDTTIAEFTINYKKTHSQTLLPMID
                     EMVKMVEADLNTIDAIAVAGGPGSFTGLRIGSATAKGLGLALGKPLIHIPTADAMAYS
                     IYGCEDIICPIMDARRNQVYTGIYSFVPKKESEDSEHGQMKYAFQVIRVQMAVSVEDL
                     IRRLNIYGRRVVFLGDGVPVYRKMLEKGLKVPYFFAPSYLNRQRAAVVGALGIRYFKM
                     GRYETAAEHKPDYLRVSQAERERAAKEKNAAPEIRRMVMEDGAAIAELEYSLFRDAWS
                     EKSILETLKQPNAICLIAEKADRTAGYLLAYTAAEEMEIARIGVVKEMRRQGVARELL
                     GALEKIGKQSGIEKILLDVRSGNEAAHKLYTSEGFTEDGIRKHFYSDPDEDAVLMSKT
                     VK"
     gene            complement(5534..5962)
                     /gene="tsaE"
                     /locus_tag="ACZPD4_01690"
     CDS             complement(5534..5962)
                     /gene="tsaE"
                     /locus_tag="ACZPD4_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1377824.1"
                     /translation="MILETNSPQETFSAGRQLGEKAFPGQVITLTGDLGVGKTVFTQG
                     LAKGLGIEEPVNSPTFTIVQVYDEGSLPLYHFDVYRIGDIEEMDEVGFEEYVMGDGVS
                     LIEWANLIEEILPENRTEVIIEKDLEKGFDYRKIIINEMK"
     gene            complement(5979..7157)
                     /locus_tag="ACZPD4_01695"
     CDS             complement(5979..7157)
                     /locus_tag="ACZPD4_01695"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1377825.1"
                     /translation="MKELELKYGCNPNQKPSRIYMSDGRELPIKVLCGRPGYINFLDA
                     FNGWQLVSELKKATGLPAATSFKHVSPAGAAVGLPLSDTLAKIYWVDDLGELSPLACA
                     YARARGADRMSSFGDFISLSDVCDVDTAKLIKREVSDGVIAPGYEPEALEILKQKKKG
                     NYNIIEIDPDYVPAALEHKEVFGITFEQGRNELNIDKDFFSDVVTENKEIPEQAKIDL
                     AISMITLKYTQSNSVCYVKDGQAIGIGAGQQSRIHCTRLAGQKADNWWLRQSPKVLGL
                     QFKDGIGRADRDNAIDLYIGDEYMDLLADGVWENTFAVKPEVFTREEKREWLDKMTDV
                     ALGSDAFFPFGDNIERAHKSGVKYIAQPGGSVRDDNVIATCNKYGMAMAFTGIRLFHH
                     "
     gene            complement(7235..7951)
                     /locus_tag="ACZPD4_01700"
     CDS             complement(7235..7951)
                     /locus_tag="ACZPD4_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR1377826.1"
                     /translation="MKMLSIEEELKNNSYPGRGVVIGKTQDGTKAVTAYFIMGRSENS
                     RNRVFVEEGEGIRTQAFDPAKLEDPSLIIYAPVRVLGNKTIVTNGDQTDTIYEGMDKQ
                     MTFEQSLRSREFEPDGPNYTPRISGVMHIENGTYNYAMSILKSNNGNPESCCRYTFAY
                     ENPVAGEGHFIHTYMCDGNPLPSFEGEPKLIGIPNDMDEFTETLWHSLNEDNKVSLFV
                     RYIDIETGKYETKIVNKNHK"
     gene            complement(8032..9351)
                     /locus_tag="ACZPD4_01705"
     CDS             complement(8032..9351)
                     /locus_tag="ACZPD4_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1377827.1"
                     /translation="MDADPAGNSILFNLLLLLFFTLLNAFFAGAEMAVVSVNKNRIRS
                     MADEGNKKAKVIEGLFEDSTKFLSTIQVAITFAGFYSSASAASGISPVLAQSLEAAGV
                     PYSSQIAYNGVTLVLMFFNLVFGELVPKRIALQKAEAFCMFTVMPVHYISIILSPFIK
                     LLSMSTKLVLKLLGMKTEDEEEAVTEEEVKALLKMGNESGTFDDDEKEMIDSVFKFDR
                     RTAREIMVPRREVIAFDIDDPFEEMIDEILETRHNRIPVYEENIDNIIGVLHVKDMMI
                     EMRKHPLKKGDVRQMLRQPFFIPETKEADELFRIMQETRHHMALLVDEYGGFSGIVTI
                     ENLVEEIMGDINEEYEEVVPEIECVREDEYKIDGGVLIDDLNDELGLKLETENYDTLS
                     GYLIEKLGHIPAKEDRDIIETDGLIFKIEEVKDNRITQVRLRMKDLK"
     gene            complement(9498..10424)
                     /locus_tag="ACZPD4_01710"
     CDS             complement(9498..10424)
                     /locus_tag="ACZPD4_01710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1377828.1"
                     /translation="MQMKKVNFGSTGKQVPAIVMGCMRMKELTVKEAAKLVDAAMAEG
                     VDFFDHADIYGGGVCEERFKEAAEEAKISREDMWIQSKCGIVPGVMYDFSKEHIIKSA
                     EDSLQRLGTDYLDSLLLHRPDALMEPEEVAAAFEELHTSGKVRYFGVSNQNTMQMELL
                     SRYVSEPLMADQLQFGAAHASMIASGVEVNMTTPGSVDRDGSVLDYCRLHDITIQAWS
                     PFQYGMIEGVFLNSDKFAVLNEKIREIAGKYGVSDTTIATVWILRHPAHMQVLSGTMN
                     LQRLREICKACEITLTREEWYAIYMAAGHMLP"
     gene            complement(10498..12423)
                     /locus_tag="ACZPD4_01715"
     CDS             complement(10498..12423)
                     /locus_tag="ACZPD4_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1377829.1"
                     /translation="MRTMFTPLKIAGMEVKNRTFMAPMSLGYESQDGTVNEKMEAYWL
                     RRAQGGVGCIIVDATSVDPNVPYLGNTLCFRDEESIKKYKSFTDKVHSYGCKIIPQIT
                     HPGPESISAFFGVAPVASSSYPNSMGQMTRELTKEELPGIVALYAKASKNAKEAGFDG
                     IELHCAHAYMLLGSFLSPLRNKRTDEYGGSLLNRARLLFEVLDAIKETCGEDFPIVLR
                     MSGSERDPQGNTLEDMKRLIPYLEKHGVDCFEISGGTQYERCNKIIPCHGEEEFTNLK
                     EARAIKAITAKPVITVGKILDAQLAEDVIETNEVDGVVLGRALLADPDFVEKAEEKRY
                     DEIAPCAGCGVGCVGEQTKRRPASCVINPLAGRETEFEEKPAESEKNVLVVGAGIGGL
                     AAARILAKRGHHVVVFEKEKKAGGQLNLACRAPFKQEISKWIVYLLQECDRYGAEIRY
                     EIEVNADVIKAENPDIVILATGAEPIVLPVEGKETMIQANDVLSGKVPILGGNAAIIG
                     GGMVGIETAEYVLHHSRGAARAALIEMTDSIGQGMVPNNLVPTMARLRQENVQMITGA
                     RVKKIDAGTIIADTKKGEMKYPGFTHVICAVGSKAWNPLYEEIKDTYETYVIGDAKEV
                     AQALEAVRGAYELGYRL"
     gene            complement(12445..>13041)
                     /locus_tag="ACZPD4_01720"
     CDS             complement(12445..>13041)
                     /locus_tag="ACZPD4_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1377830.1"
                     /translation="QVEKLVKVYKNTKVDAQMLKDADVDAVILAIGSSPITIKLEGMD
                     KLQHASAPEVLRHEVCPKGKVAVIGGGLVGLETADTLATDGCDVTVLEMKDEIGSDLG
                     SLRKIAVMMKLQQMQVKMLPNSKVCKFTEEGIVLEDETLVPCNCAVFAVGFASNDSHL
                     LVEECEKRQIPCRVIGDAKSARRALDAIAEGFEAARTL"
     assembly_gap    13042..13051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13052..14352)
                     /locus_tag="ACZPD4_01725"
     CDS             complement(<13052..14352)
                     /locus_tag="ACZPD4_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1377831.1"
                     /translation="MFEHLFAPIKIRDMELKNRIILPAMGTRMAAENGAVTDKLIAYH
                     AARAKGGCALNIVEVAAVHTPSAPAHFVSISEDYLIEGHKKLTDAIHENGGKAGIQLW
                     QGSIAACMDPKAQMLVVSDMNFGAYMIKAITKEQIQEVIACYGKAAARAVEAGYDCLE
                     FHLAHNYLPHSFLSAGFNHREDEYGGSFENRCRFPLEVIDEIRKNMPEGMPLCIRIDA
                     QDDMLENGLMIEDVIRFCKIAKEHGVDVLDVSRGNIITAASMYEVPPIDVPNGFNIDN
                     AARIRKETGMLTVGVGRINTAALAEEIISQDKVDMVVMGRAQLADPEFANKAHEGRIG
                     DIVHCVGCNQGCYDGFTDVANRPHITCMRNPMIGHESEYDLSKTETPKKVWVVGGGVA
                     GMEAAKILHERGHEVSLFEASDTLGGEFLLAGEAPGKAEMKA"
     gene            complement(14437..>15381)
                     /locus_tag="ACZPD4_01730"
     CDS             complement(14437..>15381)
                     /locus_tag="ACZPD4_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1377832.1"
                     /translation="LRCVKVSFHIGLFMFLCFEQHKENENEVLMYGVPPLFVLNAQEK
                     QRVLRVLDDLLAEEQEEEFLLYNMNIYLAMQLHALYCRYAMERKKKEDSEKDCVWDVI
                     QRVLLDTSKTVPMEEYVKAAGMTKVTLNRKIVRRCGYTFFQLQKMGKIFNACALLHFP
                     DLNIQYISDYLGFTNIEDFYRVFKRYMNVSVREYQKQNVGNGMQMHSEEEALQILVYL
                     ILNFHDPFQMKEMEKVLKKNAHLLERRAREVFGRSVLELLEEVRIQISCSYLVATDRT
                     VTQISSDVGFGSISSFQRSFFKYMNQTPSQFICSMKSK"
BASE COUNT         3788 a         4202 c         2543 g         4838 t
ORIGIN      
        1 gccgccagcg ttcatcctga gccaggatca aactctcgtt aaaagtgttt gtcttggtca
       61 agaatcacta ctagcaattc tttccctctt tactgttttg gtttgttttc aaaccgttct
      121 taaaaatttt tctctttaaa gaaattttca agggttgttg tctattgttc agttatcaag
      181 gttctttctt tgttctgtct caagcgacag cttttatatt ttatcaaaag ctttatcgtt
      241 tgtcaagaac ttttttaact tttttcattt tcttttttgt ggagctttct ctcaacgagt
      301 cagctcatct ataataccat ttgtgtcagt ttttgtcaag aacttttttg acttttttca
      361 aaaacttttc tgatattatt ttgatacgct attttcctgc ttcccgaact acggtttgca
      421 attctcaccg aatattcgac aagatttttc gcgcatcaag cggagaagga gggatttgaa
      481 ccctcgcgcc gctactaacg acctactccc tttccagggg agccccttca gccaacttgg
      541 gtacttctcc aaaaacggag agggtgggat tcgaacccac gcgccctttc ggacaaacgg
      601 ttttcaagac cgcctcgtta tgaccacttc gatacctctc catatttaca acagtgaaaa
      661 tcggcttatt gaacttcccc tgtgcaacgt acgttatgat atcacatgta catttcctat
      721 gtcaagacct tattttatat tttacagatt tttttgcttt tttccataat tgcagagcgt
      781 atttttctgc taaactgttt tttctacttt atatgatata aaataccggc tgtttctcgg
      841 aaaaatccgc aatacagccg gtgtctggcg caaaatttgt ctatgactct ctgtttctat
      901 ttttgaagag tatacttatt tgccgacaaa cgcttctgcc acctttaaat cttcgggagt
      961 tgtgattttt atattctcat aagatccctc atacaatttg accgattcac ccaacatcgt
     1021 ttccacaacc atagcatcat ccgttacagc gctttctcct tctttttcca acagccggta
     1081 tgcacgtttt atcaactccg tatcaaacac ctggggcgtc tgaacaatcc acacatattt
     1141 tctttccggg gtctgggata caatattatt gtcgtctacg attttaactg tatcttttac
     1201 aggcatccct gccgcgcagg catgttcccg caccacacat tcatatcccc tgcgaataat
     1261 attttcattc acaaacgggc gggcgccgtc atggatgtat acatatcccg cctttgtctc
     1321 ttttaaaccg ttccaaaccg aatgatatct ctgttcgcct ccgcatatga ttttctgtac
     1381 tttaaaaaag ccatatttct ccacaatctc tgtgcggcaa aactcttctt ccccttttcc
     1441 gacaacaaga atgatctcat ctataaggtc ggattgttgg aatgcctgca gggaatacgc
     1501 aagtaccggc ttttcacaca actgcaaata ttgcttatga acttttgttc ccattctctt
     1561 tccgctgcct gccgcaagca cgattgctgt acaatatact ttcgtcatag ttccaccccc
     1621 ttccgcttcc acaatctcac ctttatcttc tctgtctatg acattcttcg gctttaacac
     1681 tctttgcctc taacatccta tctttttgat attctctgtt ttaaaactct ccgcgtcttt
     1741 aacattctat ctttttggca ttctctgttt taaaactctc tgctctttaa cattctatct
     1801 ttttggcatt ctctgttttt aaactctctg cgtctttaac atctcatctc tttggtattc
     1861 tctatctttg atattttctg atttttctat cgttcaccga gttttaaatt cggcaccgca
     1921 ttcagatccc atccatgacg cgtacctgca agatattcat agaatcctgc tgcgcctatc
     1981 attgccgcat tatctgtgca ataaatcgga gacggatgat agaattgaat gccccgcttt
     2041 gcacacgccg cttccatagc cgttcttaac gccgtgttag atgcaacgcc gcctgcgatc
     2101 gcaaatttgc gcatactgta atcttccgcc gcctgcatag acttctctac caatacttcc
     2161 acaaccgctt tctgaaaaga tgcggcaata tccgcaggat caaaactttc acctttcatc
     2221 ttgcagccgt ttatataatt gagaaccgcc gacttcaaac cgctgaaact aaaatcatat
     2281 tttgcaccgt ctacatttgc tttcggaaat tttattgcat cagggtttcc ctctcttgca
     2341 attctgtcga tcttcggtcc tcccggatat cccaatccga tcgcacgcgc tactttatca
     2401 taagcttctc ctgccgcatc atcccttgtt cttccaagta tttcatactt tccgtaatct
     2461 ttcacacata caagatgagt gtgccccccg gatgcaacaa ggcacaaaaa gggcggttct
     2521 aattcgggat gctcgatata atttgccgaa atatgccctt ctatatgatg cacgccgaca
     2581 agcggaagcc ccgccgcata agcaatcgcc tttgcttctg ccactccgac aagcagcgcc
     2641 cctacaagtc ccgggccgta cgtaaccgcg accgcatcaa tatctttcaa tgttacctcc
     2701 gcctctttca gcgcttcttc aatcacctga ttgatttttt caatatgttt ccgcgatgca
     2761 atttccggaa caactccgcc atataatttg tgcagatcga tctgcgatga aataatgttt
     2821 gaaagaactt ttcttccatt gtggacaact gcagccgcag tctcatcaca cgaactttca
     2881 atcgccaaaa tattaatgtt tcttatctct ttcatttcac gcctctcaat cttcaaacat
     2941 caactccgca gtcatgccgt catttcccgc ttttgccctc ggaaaaatac ggcgcacctc
     3001 atccatataa tactgcggtt ttgtcagcga cggactgtaa tgcgtcagcc acatctcctc
     3061 tacttcggct tccttggcaa gtgccgccgc ctcatagaac gtcatatgct tgtattctac
     3121 tgcttttgcc gctttttctt gttctccgta cattccttca caaataaaca gatcagaacc
     3181 ggccgcattt tctttgatcg agtccgtagg ccgggtgtct gtcgtgtacg tcagttttat
     3241 cccttttcgc ggagggccaa gcacaagatc aggggtatag actttatcct ctgtctcaat
     3301 cgtctcgcca ttttgaagcc ggctccaata cggaagcgga attccctgtt cttttgccct
     3361 ctgagcatcg aattttccgg cgcggtcaat ttccatcgta tatccgtaac aaagtacgtt
     3421 atgatttaca cggaaagctt tcagtctgta tccgtttatc tcgaatgtct gttcagcgcc
     3481 ttctatctcc ttaaaaacga tcggaaacgg aagctcgggc gcaatcactc taaggcagct
     3541 caccacccgc tcaagtcctt ttggtccgat caatgtaagc ggctctgtac ggtctgcatt
     3601 tcccatcgta aggagcagtc ccggaagacc gcttatatga tctccgtgat aatgtgtaaa
     3661 acaaatcaca tcaatcggtt taaaactcca tcctttttct ttaatcgcaa cctgcgttcc
     3721 ttctccgcag tctataagca tactacttcc attaaaccgc gtcataagcg ccgtcagata
     3781 acgataagga agcggcatca tcccgccgca tcccaacaaa catacatcta acatttattt
     3841 ttctgtccat tctttttgtt ttaatttttc actctgcgtc aacgggtatt ttaaaagaac
     3901 tcacaagatc atcataacaa gattcgcaca gatcaaaatg atgcctttct ccgtccttct
     3961 ccgaaaaata cccccattcg cagtctgcac taaatatgcc ttcccggggt tccccgtttc
     4021 tgacaacaat ttcttttccg caccgattgc agataatcgc ctcggtttct tttatcattt
     4081 ctctttcttt caaataatat cggcgcatat cttatcctcc tgtaaataga aatttacgtt
     4141 tccgctatgc ccaaagtata tctgaatttt tttcaaattt ctatcggaaa ccgattccgt
     4201 ttttgtcggt ttttggcatt attaatttta tttaactgtt ttgctcatca aaaccgcatc
     4261 ttcatcaggg tcagaataaa aatgctttct gatcccgtct tccgtaaatc cttcgcttgt
     4321 atataatttg tgtgccgctt cgtttcctga ccgcacatcc aacaatattt tttcaattcc
     4381 cgactgtttc cctatctttt caagcgcgcc taaaagttcc cgggcaaccc cttgcctccg
     4441 catctctttc acaacgccta tacgcgcaat ttccatttct tccgcagccg tatatgccaa
     4501 aagatatccc gccgtgcggt ctgctttttc tgcgatcaga caaatcgcat tcggctgttt
     4561 cagcgtttca agaattgatt tttcactcca tgcatcacgg aacaaactat actccagttc
     4621 cgcgattgcc gctccgtctt ccatgaccat acggcggatt tccggagccg catttttttc
     4681 ttttgccgct ctttcccgct ccgcctgaga aacgcgcaga taatccggtt tatgttctgc
     4741 cgccgtttca taacgtccca tcttaaaata gcggatacca agcgctccta ccactgccgc
     4801 acgctgtctg tttaaatatg acggcgcaaa aaaatacgga actttcagac ccttttccag
     4861 catcttgcga tatacaggta ctccatcgcc caagaatacg actctgcgtc catagatatt
     4921 cagccttcgt atcaaatctt ccactgaaac cgccatctgt acacgtatta cctgaaatgc
     4981 atatttcatc tgtccgtgtt cggaatcttc actctccttt ttcggaacaa aagagtagat
     5041 tcctgtataa acctgatttc ttcttgcatc catgatcgga catataatat cctcgcatcc
     5101 gtagatactg tatgccatcg catctgctgt cggtatatgg atcaagggct tgcctaacgc
     5161 aagccccaaa ccttttgctg ttgcagatcc gatccgaagt cctgtaaacg atcccggtcc
     5221 tcccgcaact gcgatcgcat caatcgtgtt caaatccgct tctaccattt tcaccatttc
     5281 atcaatcatc ggcagaagtg tctgtgaatg tgtcttttta taatttattg taaattccgc
     5341 aatcgtcgtg tcgtcctcta ctattgcaac cgtagctgtc agacccgaac tatctattgc
     5401 caaaactctc atgctttaca tcctgctttc cttttcgctt tcttccggca ttcgtccgcc
     5461 attcttcatt cataaccgca gttcactgcc gccaaacttt ataccgtctt atccggtctt
     5521 atccgatcga acctcacttc atctcattga ttatgatctt ccgatagtca aacccttttt
     5581 ccaagtcttt ttctatgata acttccgtcc ggttttccgg aagaatctct tcgatcagat
     5641 tcgcccattc gatcagcgaa acaccgtcgc ccataacata ttcttcaaat cccacttcat
     5701 ccatctcttc gatatccccg atccggtaca catcaaaatg atacagcggc aggcttcctt
     5761 cgtcatacac ctgcacgatc gtaaaggtcg gactgtttac cggttcttcg atgccaagtc
     5821 ctttggcaag tccctgtgta aaaacagttt ttccgactcc gagatctcct gtcaaagtaa
     5881 tcacctgccc cggaaatgct ttttctccaa gctgtcgccc tgccgaaaac gtttcctgag
     5941 gactattcgt ttctaaaatc atgttacagc gcctcttctt aatgatggaa taagcgaatt
     6001 ccggtaaatg ccattgccat tccgtattta ttacatgtag cgatcacatt atcgtctctt
     6061 acagaaccgc ccggctgcgc aatatatttc acgccgctct tatgcgcgcg ttcaatgttg
     6121 tcaccaaacg ggaaaaaggc atccgatcca agagcaacat ctgtcatttt atcaagccat
     6181 tctctctttt cttctcttgt aaacacttca ggttttactg caaatgtatt ttcccacacg
     6241 ccgtccgcaa gcagatccat atactcatcg ccgatataca gatcgatcgc gttatcacgg
     6301 tccgcgcgtc cgataccgtc cttaaactga agtccgagca ctttcgggct ttgacgcagc
     6361 caccagttgt ctgccttctg tccggcaaga cgtgtacaat gtattcttga ctgctgcccc
     6421 gctccgatac cgatcgcctg tccgtctttt acataacata cagaatttga ctgtgtatat
     6481 ttcaaagtga tcatggaaat agcaagatct atcttcgcct gttccggtat ctctttgttt
     6541 tctgtaacaa catcagagaa gaagtcctta tcaatattca gttcatttct tccctgttca
     6601 aatgtgatgc cgaatacttc cttatgctca agcgctgccg gaacataatc aggatcgatc
     6661 tcgataatat tataatttcc ttttttcttt tgttttaata tttcaagagc ctccggttca
     6721 tatcccggtg cgatcacgcc gtcggaaact tctctcttga tcaattttgc ggtatccaca
     6781 tcacacacat cagacaacga aataaaatct ccgaacgaag acattctgtc tgcgcctctc
     6841 gcccttgcat atgcgcaggc cagcggagac aactccccga gatcatctac ccagtaaatt
     6901 ttcgcaagcg tatcagaaag cggaagtccg actgccgccc ctgccggaga tacgtgttta
     6961 aacgatgttg ccgccggaag tcctgttgct tttttcaact cggacacaag ctgccatcca
     7021 ttaaatgcat caagaaaatt aatatacccc ggacgtccgc aaagcacttt gatcggcagt
     7081 tcacgtccgt cactcatata aattctggac ggtttctgat ttgggttgca tccatatttt
     7141 aattctaatt ctttcatctt cttatccgct ccttataatc tttatttgcg atctttattc
     7201 gtgatcctta ttcgtaatcc ttatttcgta atctttattt atgattttta tttacgattt
     7261 tcgtctcata ctttcctgtc tctatatcga tataacgcac gaaaagagat actttattat
     7321 cttcattcag gctgtgccat aacgtctcgg tgaactcgtc catatcgttc gggattccga
     7381 tcaatttcgg ttctccttca aaactcggca aaggattccc gtcacacata taagtatgga
     7441 taaaatgtcc ctctcccgca accggattct catatgcaaa tgtatatctg cagcagcttt
     7501 ccggattccc gttattactt ttcaaaatag acattgcata gttgtacgtt ccgttttcta
     7561 tatgcattac tccggagata cgcggcgtat aattcggacc gtcaggttca aactcgcggg
     7621 agcgaagtga ctgctcgaat gtcatctgct tatccatccc ttcatagata gtatctgtct
     7681 gatctccgtt tgtaacaatc gttttattac cgaggacacg caccggcgca taaataataa
     7741 ggctcggatc ttccagtttt gccggatcaa acgcctgtgt acggattcct tctccttctt
     7801 ccacaaacac acggtttctg ctgttctcac ttcttcccat gataaagtaa gccgtcacag
     7861 ccttcgttcc gtcctgtgtt ttcccgataa cgacccctct ccccggataa gaattgttct
     7921 tcagctcttc ttcaatagac agcatcttca ttacactgta tctcctttca tctctctgtt
     7981 caaattttcg tcttcttgta aactttccca tcgtctgact cagacaggtt tttatttcaa
     8041 gtctttcatc cgcagacgga cttgcgtgat acggttgtct ttcacttctt cgattttaaa
     8101 tatcagacca tccgtttcta tgatatcccg atcttctttt gcgggaatat gccccagttt
     8161 ttcaataaga tatcccgaca gtgtatcata attttccgtt tcaagcttca gtccgagttc
     8221 atcgttcaaa tcatcaatga gaactcctcc gtcaatcttg tattcatctt ctctgacaca
     8281 ctctatctca ggaactactt cctcatactc ttcgttaata tcacccatga tctcttcaac
     8341 gagattttcg atcgttacaa ttcccgaaaa gccaccgtac tcatcgacca aaagcgccat
     8401 atgatgccgt gtctcctgca taatccgaaa cagttcatcc gcttcctttg tttccggaat
     8461 aaagaacggt tgacgaagca tctgcctgac atctcctttc ttcaacggat gttttcgcat
     8521 ctcgatcatc atatctttta catgaagcac accgataata ttatcaatgt tttcttcata
     8581 taccggaatc cgattatgtc ttgtctcaag gatctcatcg atcatctcct caaacggatc
     8641 gtcaatatca aatgctatca cttctcgcct cggaaccatg atctctcttg cagtccttct
     8701 gtcaaactta aacaccgaat cgatcatctc tttttcgtca tcgtcaaatg taccgctttc
     8761 atttcccatt tttaaaagcg ctttcacttc ctcttccgtc accgcttctt cctcatcttc
     8821 cgttttcatc ccaagaagct ttaacacaag cttcgtagac atggaaagaa gtttgataaa
     8881 cggagacagt atgatcgata tatagtgtac cggcattacc gtaaacatac aaaacgcttc
     8941 cgctttctgc agcgcgatcc tcttcggaac aagctcgccg aacacaaggt taaaaaacat
     9001 cagcacaagc gtcaccccat tgtaagcaat ctgcgaactg tacggaacgc ctgcagcctc
     9061 aagtgactgc gccagaacag gcgaaatccc cgacgctgcc gatgccgacg aataaaatcc
     9121 cgcaaatgtg atcgcaacct gaatcgtcga aagaaacttc gtcgaatcct caaataaacc
     9181 ttcgatcact tttgcctttt tatttccttc atccgccata cttcgaatcc ggttcttatt
     9241 caccgatacg accgccatct ccgccccggc aaaaaaagcg ttcagcaaag tgaaaaataa
     9301 caacaacagc aagttaaaca aaatagaatt ccccgcaggg tctgcgtcca taaaaaatac
     9361 tcctcctgaa ataaattatt ttcaggagaa gtatatcatg cgcctatcat tttagcaagt
     9421 tgacgccggt atttttgcat tcgccttttt acacacacgc tttaacttca tattcgttgt
     9481 ccagagaaag ccgcagttta tggaagcata tgacctgccg ccatatagat cgcataccat
     9541 tcttcccgtg taagagtgat ctcgcatgcc ttacagatct ccctaagcct ctgcagattc
     9601 attgtgccgg aaagaacctg catgtgcgcg ggatgtctta aaatccacac ggtggcgatc
     9661 gttgtatcgc tgaccccgta ctttcctgca atctcgcgga ttttttcgtt tagaaccgca
     9721 aacttgtcgc tgtttaaaaa cactccttcg atcattccgt actgaaatgg cgaccatgcc
     9781 tgaatcgtaa tgtcatgcag ccggcaataa tctaatacgc ttccatcccg gtcaacgctt
     9841 ccaggcgtcg tcatatttac ttccactccg ctcgcgatca tggacgcatg agcagcgcca
     9901 aactggagct gatccgccat aagcggctca ctcacatatc tgcttagaag ctccatctgc
     9961 atcgtattct gattcgatac gccgaaatat ctcactttcc ccgacgtatg cagctcctca
    10021 aaagccgccg ctacctcttc tggttccatg agcgcatccg gtctgtgaag aagaagagaa
    10081 tccagataat ctgttccgag tctttgcaga gaatcttctg ctgattttat gatgtgctct
    10141 ttagaaaaat cgtacatcac ccccggcaca attccgcatt tgctttgaat ccacatatct
    10201 tcacggctta ttttcgcttc ttccgccgcc tctttaaaac gctcttcgca gactccccct
    10261 ccgtaaatat ccgcatgatc aaaaaaatcg accccctctg ccattgccgc atcaaccagc
    10321 ttcgccgctt cttttacggt cagttctttc atgcgcatac atcccatgac aatagccgga
    10381 acctgttttc ccgtacttcc gaaattcact tttttcatct gcatttccac ctctttcttc
    10441 cttgctgtta cttatattgt aaacgctgta aacgatagtc gtttcccatg ttggatatca
    10501 taaccgatat ccaagttcat atgctccacg cacagcttcc agcgcctgcg ccacttcttt
    10561 tgcatctccg atcacatatg tttcataagt gtctttgatt tcttcataca acggattcca
    10621 tgcctttgag ccaaccgcgc atatcacatg tgtaaatccc ggatatttca tctcaccttt
    10681 ttttgtatct gcgataattg ttcccgcatc aatctttttt actcttgcgc ctgtaatcat
    10741 ctgtacattt tcctgtctga gacgagccat agtcggcaca agattattcg gaaccattcc
    10801 ctgacctatg ctgtctgtca tctcgataag agctgctctt gccgctcctc tggaatgatg
    10861 aagcacatat tccgccgttt ctattccaac cattcctcct ccgataatcg ctgcatttcc
    10921 gccgagaatc ggaacttttc cgctcaatac gtcatttgcc tgaatcatag tttccttgcc
    10981 ttctaccgga agtacgatag gttctgctcc tgttgcaaga attacgatat ccggattctc
    11041 cgctttgatc acatctgcat ttacttctat ctcatagcga atctctgcac catatctgtc
    11101 acattcctgc aaaagatata cgatccattt tgaaatttct tgtttaaacg gagcgcggca
    11161 tgccagattc aactgaccgc ctgctttttt ctctttctca aatacgacca catgatgtcc
    11221 gcgttttgca agaattcttg ctgcggcaag tccgccgatt cccgctccga cgaccagaac
    11281 attcttttcg gattccgccg gcttttcctc gaactctgtc tctcttcccg ccaacggatt
    11341 gatcacgcaa gacgccggac gtcttttcgt ctgttcgccc acacatccga ctccgcagcc
    11401 cgcacaagga gcgatctcgt catagcgttt ttcttctgct ttctccacaa aatccggatc
    11461 tgccaaaaga gcacggccaa gcacaactcc gtcaacttca tttgtttcaa tcacatcttc
    11521 cgccaactgt gcatccagga tttttccgac cgtaatcacc ggttttgcag ttatcgcttt
    11581 aatcgcccga gcctctttta agttcgtaaa ctcttcttct ccatgacatg gaataatctt
    11641 attgcagcgc tcgtactgtg ttcctccact gatttcaaaa caatcgactc cgtgtttttc
    11701 cagataaggg atcagacgtt tcatatcttc caatgtattt ccctgtggat ctctttcgct
    11761 tccggacatt cttaaaacga tcgggaaatc ttctccgcag gtttctttga tcgcatccaa
    11821 tacttcaaac aacaaacggg cgcgattcaa cagacttcct ccgtattcat ctgtacgttt
    11881 atttcgtaaa ggtgacaaaa acgatccaag aagcatatat gcatgggcac aatgcagttc
    11941 tatcccgtcg aacccggcct ctttcgcatt tttagatgct tttgcataaa gagcgacaat
    12001 tcccggcagc tcttctttgg tcagttccct tgtcatctgt cccatagaat tcggataaga
    12061 actgctggcc accggcgcca ctccgaaaaa tgcactgata ctttccggac ccggatgcgt
    12121 aatctgcgga atgatcttac agccataaga atgaactttg tctgtaaagc ttttgtattt
    12181 tttgatactt tcttcatccc gaaaacaaag tgtatttcca agatacggaa cattcggatc
    12241 aacacttgta gcgtctacga tgatacatcc cacaccgcct tgcgcacgcc tgagccagta
    12301 agcttccatt ttctcattca cagtaccatc ctgactttca tatcccagcg acatcggagc
    12361 cataaaagtt ctgtttttca cttccattcc cgcaatctta agcggggtaa acattgttct
    12421 catacgcttt atcctcctat atttctacag tgttcttgcc gcttcaaatc cttccgcgat
    12481 cgcatccaga gcacgtcttg cggattttgc gtctccgatc acgcggcacg gaatctgcct
    12541 tttttcacat tcttccacaa gtaaatgtga atcatttgaa gcaaatccaa cagcaaatac
    12601 cgcgcaatta cacggcacaa gagtttcatc ttccagcaca attccttctt ccgtaaattt
    12661 acacactttg ctgtttggaa gcattttcac ctgcatctgc tgcaatttca tcataacggc
    12721 aatcttacga aggctgccca gatcgcttcc gatctcatct ttcatttcca aaacagttac
    12781 atcacatccg tctgtcgcaa gagtatccgc tgtttccagt cctaccagac cgcctccgat
    12841 aacagcgact tttcctttcg gacacacttc atgcctgaga acttccggtg ctgatgcatg
    12901 ctgcaactta tccatacctt ctaacttaat cgtaatcgga gaagaaccga ttgccaaaat
    12961 aacagcgtcc acatctgcat ccttcaacat ttgtgcatct acttttgtat tcttatatac
    13021 ctttacaagt ttttctacct gnnnnnnnnn ngccttcatc tcagctttgc ccggcgcttc
    13081 tccggcaaga agaaattctc ctcctaatgt gtcagaagct tcaaacagcg aaacttcatg
    13141 tcctctttcg tgcaggatct tcgcagcctc cattccggca acacctccgc cgacaaccca
    13201 tactttcttc ggagtttccg ttttacttaa atcgtactct gattcatgac cgatcatcgg
    13261 atttctcata caagtaatat gaggacggtt tgcaacatcg gtaaagccat catagcagcc
    13321 ctgattacat cctacgcaat gtacaatatc gccgattctg ccttcgtgcg ccttattcgc
    13381 gaattccgga tctgcaagct gcgcccttcc cataacaacc atatctactt tgtcctgaga
    13441 tatgatctct tctgccagcg ccgccgtatt gatccttcct actccgacag tcaacatccc
    13501 tgtctctttt cggattctcg ccgcattgtc aatattaaat ccatttggca catcaatagg
    13561 cggaacttca tacatagacg ccgccgtaat gatatttcct ctggatacat ctagaacatc
    13621 aacgccatgc tcttttgcaa tcttacaaaa tcgaatcaca tcttcaatca taagaccgtt
    13681 ttccaacatg tcgtcctgag cgtcaattct gatacacaac ggcatccctt ccggcatatt
    13741 tttgcgaatt tcatcaatta cttccaacgg gaatcgacat ctgttctcaa aacttcctcc
    13801 atattcatct tccctgtgat tgaagcccgc actcagaaat gaatgaggaa ggtaattgtg
    13861 cgccagatga aattccaagc agtcatatcc ggcttctacc gctctggcag ccgcttttcc
    13921 atagcatgcg attacttcct gaatctgttc ttttgtaatg gctttgatca tataagcgcc
    13981 aaaattcata tccgaaacaa ccagcatctg cgccttcgga tccatgcatg cggcaataga
    14041 tccctgccaa agctgaattc ctgctttccc gccgttttca tggattgcat ctgtcagttt
    14101 tttatgtcct tcaatcaagt aatcttcact gatagataca aaatgcgccg gcgcacttgg
    14161 cgtatgtacg gcggctactt ctacaatatt cagcgcgcat ccgccttttg ctcttgcagc
    14221 gtggtaagca atcaatttat ctgtcactgc cccgttttcc gccgccattc tcgttcccat
    14281 agccggcaaa ataattctgt ttttcagctc catatcacga atcttaattg gagcaaataa
    14341 atgttcaaac atctttctgt cacctccggt attttgattt cattataagg cgaagtacag
    14401 aaaatagata ttcagatatt ttaaaattat tatcaattat ttacttttca tactacatat
    14461 gaattgactt ggcgtttgat tcatatattt aaaaaaactg cgctgaaaag atgaaatact
    14521 tccaaatcca acgtcactgc tgatctgtgt aacagtccgg tctgtagcaa caagataact
    14581 gcaggaaatc tgaattctta cttcctccaa caattccaaa acacttcgtc caaacacctc
    14641 ccgcgctctt ctttcaagca aatgcgcatt ctttttcaac actttctcca tttctttcat
    14701 ctgaaacgga tcatgaaaat taagtataag ataaaccaaa atctgcagtg cctcttcctc
    14761 cgaatgcatt tgcataccgt ttcctacatt ttgtttctgg tattctctga cgctcacgtt
    14821 catataacgt ttaaatactc tgtaaaaatc ttcgatatta gtaaatccga gataatcact
    14881 gatgtattgg atattcaaat ccggaaaatg cagaagggca caggcattaa atatctttcc
    14941 cattttctga agctgaaaaa aagtataacc acaccgccgc acaattttac gattcaatgt
    15001 tacctttgtc atccctgccg ctttgacata ctcttccatt ggtactgttt tgcttgtatc
    15061 caacagcacc ctttgaataa catcccatac acagtctttt tcggaatcct cttttttctt
    15121 tctctccata gcgtatctgc agtacaatgc atgtaactgc atggcaaggt agatattcat
    15181 attatacagc agaaattctt cttcctgctc ttcagccaac aagtcgtcta aaacacgaag
    15241 aaccctctgt ttttcttgtg cattcaaaac aaataaaggg ggaacgccat acatcagcac
    15301 ctcattttca ttttctttgt gctgttcaaa acacaaaaac ataaacagac cgatatgaaa
    15361 agaaaccttt acgcagcgca a
//
LOCUS       DBMDXF010000021        15358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1140_length_15358_cov_2.231500, whole genome shotgun sequence.
ACCESSION   DBMDXF010000021 DBMDXF010000000
VERSION     DBMDXF010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 15358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15358
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_15358_cov_2.231500"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2349)
                     /locus_tag="ACZPD4_01735"
     CDS             complement(<1..2349)
                     /locus_tag="ACZPD4_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HIR1377833.1"
                     /translation="MRGMNQKRKRLGLWILFLAALLLTQFTVPVKASEPQIQDVNIQM
                     VGGQIRTIDPMGLRMVAYIKKSYIQELEKSGATVSYGIVLLPKKYLTEGQALTLDGKY
                     LYNGSVYKPAKVPAVKKFSEDNDRIYFTAVLANLPKERYKNDYAARAYAEITRTVTED
                     GGKKKTTTEVVYSESEIDRQVYRIAEEAVNGTTETEETKQWLQDNILAPVDTPEELPE
                     EEKKISFRLGKVSGVTLYHKTTSEAGVETVEEVSQFNLTEFKKEDYLVKVEMEDQPEI
                     FAGITEVIAPQTETGKVSFKLDRKDYTTSQAGQTIEGAVVEFGTKSNDKVSTKYITMQ
                     SLIDQMKANPGGTYTLEHDIDASMVQGDDYLVPTFSGTFNGNGYKIKGLTTTLFGTVS
                     GGKVQNVKLENVSITKVNSYKDAGGGTIANKAQKDAVIENVHVSGSLKSTNSRELLGG
                     LVGRMDYAKVSKCSANLEITGSFNTTGGLIGQMSNQNEGPNIVENSYAVGSIRGNRTN
                     GALGGLIGWHNCKTNFSVTNCYAAINMELTGTNRQPGGFIGYIGEADATGVLKSNVSY
                     STGNAGYKFDGSTETIKYTTAQIENLYSLRESRLKRESSRTGNTNLTQITDVTVDKLS
                     QKEFYTNMGWSEDVWDFAPLKEGKTPILRNNDSNMTTMLQTKEIASAADLKNIKNDLS
                     GVYVLTTDIDISESASGTAVIPGIFKGTLKGNGHQIIGQKIPLFDTLDGATIENVKLV
                     QGEINQKGTDKVAALAKTSQADTLIKEVYVRDMSVTGQSNVAG"
     gene            complement(2359..2955)
                     /locus_tag="ACZPD4_01740"
     CDS             complement(2359..2955)
                     /locus_tag="ACZPD4_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377834.1"
                     /translation="MKMQKNKKLVQAAAVGLSVCVLAGGGLFVWNMYGKNAEENEKQI
                     VSDEVAEIESLNSRDNVVDLEELFEGEKASGKEDVTSEKTEKKEDSSTKSGSSQKSDS
                     SKQSGGAKKPESSKDTDTSKKPDKKPSKPDEKPSKPDEQPSEPDDKPSVPDEKPSVPD
                     EKPSVPDEKPSVPDEQPSTPQKPALPQVNEPGWITGIY"
     gene            complement(3198..4940)
                     /locus_tag="ACZPD4_01745"
     CDS             complement(3198..4940)
                     /locus_tag="ACZPD4_01745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377835.1"
                     /translation="MKNTTIFKRLLGYLKAYRLRLFFVLLSAVISTAFMVLAPFLVGK
                     VTTTLFASIADKVFYWETIIWLLAALVALYFVSQLFSFLQGFGMAKITANVMQTIRRE
                     IDEKMHRLKLNYYDVHTHGDILSVITNDVDTINNTISQNLTSIVTQVTTAIGVLIMML
                     MISPKLSLIPIVMVPLSLLSAAGVMKASEKYYGEQQELLGKLNGYVEEMYNGQSVVQT
                     FNYQERAKKKFSQLNDALKNSSRKAETTAGAISPITTLVNDLGYVLCAALGCLWAIAG
                     KIAVGNVQAMLEYTWRFAEPFSAIAGMVGSFGAAAAAGNRIFGLLDAEEEIPDSDQCI
                     VPDDRSGRVTFENVTFGYTPDHLLMNGIDLTVEPGQKVAVVGPTGAGKTTLINLLMRF
                     YEVNNGSIKVDGTKITDMPREELRDRFGMVLQDTWLFEGTIGENIGYAEDNMKKEKIV
                     EAAKSACAHSFIKTLPGGYDMTLSKGAENISQGERQLLTIARAIASDPEIMILDEATS
                     NVDTHTEVLIQKAMAELMKGRTSFVIAHRLSTIRDADMILYMEDGDIKEVGNHEELMK
                     KNGKYAALYMSQFA"
     gene            complement(4942..6666)
                     /locus_tag="ACZPD4_01750"
     CDS             complement(4942..6666)
                     /locus_tag="ACZPD4_01750"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1377836.1"
                     /translation="MKTIFSKVKLRPGQIVQLFLLVLLSAVGQMLLPSFLAQMISHGV
                     AEGENRIVWMYAVIMAGVTLFSCVISFLSVKIASYISTDFAAQLRTQVFSKVQEFSAA
                     EMDRFGTASLVTRSTSDITNVQNFLTLLLRVGLLAPMMAAAGLVFSAATGGEVSSVLL
                     VAIPVLLTVLTIIMVLASKYSIRLRKKLDQINRLFLESLEGVRVIRAFNKQKTENARF
                     EEANEDYAMTAMAAGRITSLLMPAISVIFGVTTAAVLGMGAYYVSTGAMEVGSLVANS
                     QYISMVLTSVMMLSLVIMMFPTSYACAKRIAEVLQTDSSIRGGKFQMENRPVRGTVEF
                     RHVTFAYPGADEPILKDISFESRSGEVTAIIGGTGRGKSSILKLIPRLYDPMFGEVLI
                     DGVNAKEYRTEDLRSLIGYVPQKNVLFSGDIADNLNFGNENGTEEDWKQAAKTACADE
                     FICKKKEGYHDSVAQGGTNLSGGQRQRMAIARAMMKKPEIYVFDDSFSALDMKTDRQL
                     RQNLKESIEDATVIMVAQRISTVLDADRILVVDDGQIVGNGTHRELLDTCPLYREIAE
                     IQLGKEAL"
     gene            complement(6663..7238)
                     /locus_tag="ACZPD4_01755"
     CDS             complement(6663..7238)
                     /locus_tag="ACZPD4_01755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1377837.1"
                     /translation="MEFICAEEKQWKEIKTIYMEAFPKRERKPYFALRHSVKTKKAVV
                     MAATEGEQVLGFIVLIPYKDMVMADYLAVSSKVRSRGTGSYIMQNVCRQYKDKKIVLL
                     IERLDDKAENKEQRIARRKFYLKNGFTSSDIFITGASGEMEIMNFGEKVSEEAYIDLQ
                     KYALGTVMFRLSKIKVAEKTPHKKREEADQG"
     gene            7330..8139
                     /locus_tag="ACZPD4_01760"
     CDS             7330..8139
                     /locus_tag="ACZPD4_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1377838.1"
                     /translation="MNRKNYLYSSGEFAKLTGVNKRTLHYYNEIGLFRPEIIGENGYH
                     YYTCFQFTELELILTLRKIGLSIDEIKDYSSRPSDESFTEMMTKKKKLIDESIEQLLS
                     AQAFLEQKAERLKIGMYSQHGTIERCTLPERKIIFSSPISGEYDEDDFSIAAEFSLRL
                     KKLFHLYDNFGSCISIEDIQKNSFNSYHSFFAYCPQTSDVYDEILPAGTYLRAFCIGD
                     WGKLPEIYRSILYYARKHNLTLTGYAYEEGLNEMSIKDRSDYITMITIKCD"
     gene            complement(8126..9169)
                     /locus_tag="ACZPD4_01765"
     CDS             complement(8126..9169)
                     /locus_tag="ACZPD4_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR1377839.1"
                     /translation="MLAYTYEENGKFVLKEKEKPTLQSEDDAIVRVTLASICSSDLHI
                     KHGAVPRAVPGITVGHEMVGIVEETGRRVTKVKPGDRVTVNVETFCGECFFCKNGFVN
                     NCTDQNGGWALGCRIDGGQAEYVRVPYAEQGLNKIPDSVTDEAALFVGDVLATGFWAA
                     RISEIGEEDTVVIIGGGPTGICTLLCVMLKNPEKIILCEVDPKRIAFVKEHYPDVLVV
                     NPKETDAERFVKENSRHNGADRVLEVAGSSDTFELAWKVARPNAIVTVVALYEENQTL
                     PLPQMYGKNLIFKTGGVDGCDCDEILKLIEQGRIDTTPLITHTFPLEEIEQAYDIFEN
                     RKDNVIKIAVSHI"
     gene            complement(9212..9490)
                     /locus_tag="ACZPD4_01770"
     CDS             complement(9212..9490)
                     /locus_tag="ACZPD4_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HIR1377840.1"
                     /translation="MAKREKNPASDKTGNDTGISLLVLGIVSVIFALWIPIVGIVLGT
                     VSLVRVRKRNDLSGTAMAKAGKVLSIAGICIAVVNWILAIVLTFMTLA"
     gene            complement(9657..11813)
                     /gene="gnpA"
                     /locus_tag="ACZPD4_01775"
     CDS             complement(9657..11813)
                     /gene="gnpA"
                     /locus_tag="ACZPD4_01775"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HIR1377841.1"
                     /translation="MENSKGLVTIPTDLDVVPETLELMKRWGADAIRDCDGTDFPEAL
                     KDADAEIYSTYYTTRKDNEWAKANPEEIQQMYLMTPFYTADSESLSIEIMKGLYPDML
                     SPNTRDDIKRWWEVVDRTTGKVVPTDEWDYNEEEGKVTIKAVPFHEYTVSFLAYIMWD
                     PVHMYNAVTNDWKDVEHQITFDVRQPKTHEYTLKRLRKFIEDHPYVNVLRFTTFFHQF
                     TLVFDELAREKYVDWYGYSASVSPYILKQFEEEVGYPFRAEYIIDQGYYNNQYRVPSK
                     EFQDFQAFQRREVAKIVKEMTEITHECGKKAMMFLGDHWIGTEPFMEEFKTLGIDAVV
                     GSVGNGSTLRLISDIEGVKYTEGRLLPYFFPDVFNENGDPVKEAKYNWVTARRAILRK
                     PIDRIGYGGYLKLALQFPEFLDYVEQVCNEFRTLYANVKGTTPYCVKKVAVLNCWGKM
                     RAWGCHMVHHALYQKQNYSYAGIIESLSGAPFDVVFINFQDILDNPAILDDIDVIINV
                     GDADTAHTGGEWWENPKIIEAIRGFVYNGGGIIGVGEPSGHQYQGHFFQLANVFGVEE
                     ETGFTLGYDKYNWDEHEHFILEDSEEVDFGEGKKNIYALPSATILVQKEKEVQMAVNE
                     FGKGRAVYISGLPYSFENSRVLYRAVLWSTHSEDELNRWFSTNYNVDVHAYPKNNKYC
                     VVNNTDEPQQTTIYRGDGSSFELALEPNQIIWYEIA"
     gene            complement(11871..12050)
                     /locus_tag="ACZPD4_01780"
     CDS             complement(11871..12050)
                     /locus_tag="ACZPD4_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6903 family protein"
                     /protein_id="HIR1377842.1"
                     /translation="MDERTKQVLVGIIVAVIFIASVALIIIGQKNIGPQGLLTMLAGL
                     AGLIGLLAFYNHKYK"
     gene            complement(12063..12956)
                     /locus_tag="ACZPD4_01785"
     CDS             complement(12063..12956)
                     /locus_tag="ACZPD4_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1377843.1"
                     /translation="MGKTKVKESKKPLTLSKVVVYILLILLAITIIIPVGWVFVASVK
                     QNSEFYGSPWVLPEKIYFQNFIDAWEKASMGTYMLNSVIVTALALLLLIVISLPAAYV
                     LSRFKFKTSKFWNVLFMAGLFINVSYIVVPIFLMLNNWDKTLRQLTGGTFFLNNLFVL
                     ALVYASTALPFTIYLLSGYFRSLAKDFEEAAYVDGASYFTTMVKIIFPMAKPSIVTVI
                     LFSFLSFWNEYIISMTLLTKPELKTLPVGLMNLMAAQKSAVQYGQMYAGLVIVMLPTL
                     ILYICVQKKLTQGMTLGGLKG"
     gene            complement(12972..13862)
                     /locus_tag="ACZPD4_01790"
     CDS             complement(12972..13862)
                     /locus_tag="ACZPD4_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1377844.1"
                     /translation="MKARKGKGLFVFCCLAPAAILFIVFMILPTIDIFRMSMFKWGGY
                     TDNKTFVGLDNFKILFKSDKFYQAFQNSILLIVLVTLVTFTFALVFAYILSREKIKGK
                     TFFSVIFYIPNILSVVVISAIFSAIYDPNLGLLNSFLNLFKGAENPTLWLGDQKIVIY
                     SIVIALVWQAIGYYMVMYMASMANVPESLYESAALEGAGKIRQFFTITIPLIWTNIRT
                     TLTFFVISTINMSFLLVKAMTNGGPDGASNVFLSYMYQEAYTNSSYGYGMAIGVMVFL
                     FSFALAGILNVATKRQEIEF"
     gene            complement(13979..15319)
                     /locus_tag="ACZPD4_01795"
     CDS             complement(13979..15319)
                     /locus_tag="ACZPD4_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1377845.1"
                     /translation="MKKKKLISLLLVASMAFSLAACGSSGGDDKDADKGGDEKTLKVA
                     AVETAYGADMWKEVCSAFEKSHEGVKVELTIDKKLEDVINPQMKSGEYPDVILRAVGA
                     ESGITETFVKDNNLVDLTDVLDMTVPGEEVTVGDKILPGFVENTITNPYGDGKTYLMP
                     MFYGPCGLFYNAGLFEEKGWDVPTTWDEMWALGDKAKEEGIALFTYPTTGYFDAFFYA
                     LMHETMGDDFSKALTYEDGIWDTEGAKQAFDIVAKLATYTEKTTPANANDNDFRKNQQ
                     LVLDNKALFMPNGNWVIGEMEDAPRADGFEWGFTALPAVKEGGERASYTFFEQIWMPA
                     EAKEQDLGKEFIAYLYSDEAAKIFFDKGGAVQPIEGMSDMITDDNTKLYYSIYDTGAV
                     AVMDAFAATEPIEGLTTRSTFFDPVNSLVTGDKTEQDWIDQIKADSAKFHEALK"
BASE COUNT         3900 a         3957 c         2771 g         4730 t
ORIGIN      
        1 acctgctaca ttactttgtc ccgtaacgct catatctctg acatatactt ctttgataag
       61 agtgtctgcc tgtgaagttt ttgccagagc cgcaacttta tctgttccct tctgattgat
      121 ctcgccttgt acaagcttca cgttttcaat cgtcgctcca tctaacgtat cgaacaacgg
      181 aatcttctga ccgatgatct gatggccgtt tccttttaat gttcctttaa atattcccgg
      241 gataaccgct gttcccgacg ctgattccga tatatcgata tctgtagtca gaacatacac
      301 acctgacaaa tcatttttaa tattcttcag gtcggctgcc gatgcgattt cctttgtctg
      361 gagcattgtc gtcatatttg agtcattgtt tctaaggatt ggagtttttc cttctttcag
      421 aggagcaaaa tcccaaacgt cttcactcca gcccatgttc gtgtaaaact ctttttgtga
      481 aagtttatcc actgtaacat ctgtgatctg agtcagattc gtatttccgg tacgactact
      541 ctctcttttc agtctgcttt ctcttaaact ataaagattt tcaatctgtg ccgtagtata
      601 tttgatagtt tcagttgatc catcaaactt atatcctgca tttccggtac tgtatgaaac
      661 attgctcttt agtacacctg tcgcatcagc ctctcctata tatccgatga atcctcctgg
      721 ctgtctgttg gttccagtca gttccatatt gatagcggca taacaatttg ttactgaaaa
      781 gtttgtttta cagttatgcc atccgatcag tccgccaagc gccccattcg ttctatttcc
      841 tcgaattgat ccaacagcat atgaattttc tacaatatta ggaccttcat tttgattgga
      901 catttgaccg attagtccgc ccgtcgtatt gaaagatcct gtaatctcca gattagcaga
      961 gcatttactt actttcgcat aatccatacg ccctacaaga cctccgagaa gttctcggct
     1021 gtttgtactt ttcaaacttc cacttacatg tacgttctcg atcacagcat ctttctgagc
     1081 tttattcgct attgttccac cacctgcatc tttataactg tttacttttg taatggaaac
     1141 attttcaagt ttaacatttt gtaccttacc tccagaaacc gttccgaaca gagttgttgt
     1201 cagacctttg atcttatatc catttccgtt aaacgtaccg ctaaatgtcg gaacaaggta
     1261 atcatctccc tgaaccatac ttgcgtctat atcatgctcc agagtataag ttccgcctgg
     1321 atttgccttc atttgatcga tcagcgactg catcgtaata tattttgtag atactttatc
     1381 gttagacttc gtaccaaact ctacaaccgc gccttcgatc gtttgtcctg cctgagaagt
     1441 agtataatct tttctgtcaa gtttaaaact tacttttcct gtttctgtct gcggtgcgat
     1501 cacttccgta attcccgcaa atatctcagg ctgatcttcc atctcaactt tcacaagata
     1561 atcttctttt ttaaattctg taagattaaa ctgtgatact tcttctacag tttccactcc
     1621 ggcttctgac gttgtcttat ggtacagtgt cacaccggac acttttccaa gacgaaacga
     1681 gatctttttt tcttcttccg gcagttcttc cggcgtatct accggagcga gaatgttatc
     1741 ttgaagccac tgttttgtct cttccgtctc tgtagttccg tttacagcct cttctgcaat
     1801 ccgatatacc tgacggtcga tttcactctc actgtataca acttctgtcg ttgttttttt
     1861 ctttccgccg tcttctgtca cagttctcgt gatttctgca taagctctcg ccgcgtaatc
     1921 atttttatat ctttctttcg gcagatttgc caaaacggct gtaaagtaaa tcctatcatt
     1981 gtcttctgaa aatttcttta ctgccggtac ttttgccggc ttatagaccg agccgttata
     2041 aaggtacttg ccgtcaagcg tcaatgcctg accttctgtc aaatactttt tcggaagcag
     2101 tacgattcca taacttaccg tcgctccgct tttttccaac tcttgaatat atgatttctt
     2161 gatgtaggca accattcgca gccccatcgg atcgatcgta cgaatctgcc ctccaaccat
     2221 ctgtatgttt acatcttgta tctgcggctc ggatgcctta accggaacgg tgaactgcgt
     2281 caaaagcagc gccgccagaa aaagtatcca taaccctaat ctctttctct tttgattcat
     2341 ccctctcata ttccttcctt aatatatacc tgtgatccac cccggttcat tcacctgcgg
     2401 caatgccggt ttctgcgggg ttgacggttg ttcatccggt acggacggtt tctcatccgg
     2461 tacggacggt ttctcatccg gtacggacgg cttctcatcc ggcacagacg gcttgtcatc
     2521 cggttctgac ggttgttcat ccggttttga tggcttctcg tccggtttgg atggtttctt
     2581 atccggcttc tttgatgtat ctgtatcttt tgatgactcc ggtttttttg ccccgcctga
     2641 ttgcttagaa gaatccgact tttgagaaga gcctgacttc gtcgaagaat cttctttctt
     2701 ctctgttttc tcgctcgtca catcttcttt tcccgacgcc ttttctccct cgaaaagctc
     2761 ctccaaatct acaacattgt cgcgtgaatt caagctttca atttccgcaa cttcatctga
     2821 tacgatctgc ttttcatttt cttctgcatt tttgccgtac atattccata cgaacagacc
     2881 gcctcctgct agcacacaga ctgaaagtcc taccgcagca gcttgcacaa gtttcttgtt
     2941 cttctgcatc ttcatgcttt ccctccttac aaaatatacc tttacataaa tataaccgga
     3001 gagttgtgga atagatcacg caagactccg gttgttattc tgttacacta tttttcccat
     3061 ttgataatat actttctttt tttactttct gcaagcgttt tttacaaaaa acaacgaatt
     3121 tccagtattt tacgaatagt gcatctttcc cctctatttc agttcctttg ccccgctgta
     3181 aaatttctat aatccggtta cgcaaactga ctcatataaa gagccgcata tttcccgttc
     3241 tttttcatca gttcttcatg atttccgact tctttgatat ctccgtcttc catataaagg
     3301 atcatatctg cgtcacggat cgtagaaagt ctgtgggcga tcacaaaact tgtgcgtcct
     3361 ttcatcagtt ctgccatcgc tttttgaata agaacttccg tatgggtatc tacgttactt
     3421 gtcgcctcgt ctaagatcat gatctcagga tcggaagcga tcgctcttgc gatcgtcaga
     3481 agctggcgtt ctccctgcga aatgttctcc gctcctttac taagcgtcat atcgtatccg
     3541 cccgggagtg ttttgatgaa actgtgggcg catgccgatt tcgcggcttc tactattttc
     3601 tctttcttca tattatcctc ggcatatccg atattctctc cgatcgttcc ttcaaacagc
     3661 catgtatcct gaagaaccat accgaatctg tcgcgcagtt cttcccttgg catatccgtg
     3721 atcttagttc cgtccacttt gatcgatccg ttatttacct cataaaagcg catcaaaaga
     3781 ttgatcagcg ttgtttttcc cgctccggta ggtcctacta ccgcaacttt ctgccccggt
     3841 tctaccgtca aatcgatccc gttcatcaaa agatgatccg gcgtatatcc gaacgtcaca
     3901 ttttcaaatg taacgcgacc gctgcggtca tcaggtacga tgcactggtc tgaatccgga
     3961 atttcctctt ctgcgtcaag cagtccgaaa atacggtttc ctgctgccgc tgccgctccg
     4021 aaacttccta ccattccggc gattgcggaa aacggctctg caaaacgcca tgtatactcc
     4081 aacatagcct gtacgtttcc gactgcgatc tttccggcga tcgcccagag acatcctaaa
     4141 gcggcgcaaa gaacataccc aagatcattc acaagagttg taatcggact gatcgcccct
     4201 gctgttgtct ccgcttttct tgaactgttt ttaagcgcgt cattcaactg agaaaatttc
     4261 tttttcgcac gttcctgata attaaacgtc tgcacaacgg actgtccgtt atacatctct
     4321 tctacatatc cgttcagctt accgagaagc tcctgctgtt ctccataata cttttcgctc
     4381 gctttcatca ctcctgcggc gctcaataga gaaagaggca ccataacaat cgggatcaaa
     4441 gacagcttcg ggctgatcat cagcatcata ataagtacgc cgatggcagt cgtaacctgt
     4501 gttacgatcg aagttaagtt ctgactgatc gtattgttga tcgtatctac atcgtttgta
     4561 attacactga gaatatctcc gtgtgtatga acgtcataat aattcagttt cagccgatgc
     4621 attttctcat cgatttccct gcggatcgtc tgcatcacat ttgcagtgat ctttgccata
     4681 ccgaatccct gcaaaaagga aaacaactgt gatacaaaat ataacgctac caacgccgcc
     4741 aaaagccaga tgatcgtttc ccagtaaaaa actttatcgg caatacttgc aaacaacgtc
     4801 gtcgtcacct ttcctacaag aaacggagca agaaccataa aggcagtgct gataactgct
     4861 gaaagcagta caaaaaataa ccgaagtctg tacgctttta aatagcccag cagcctctta
     4921 aatattgtcg tatttttcat atcaaagcgc ctcctttcct aactgaatct ctgcaatttc
     4981 ccgataaagc ggacaagtat ccaacaattc cctgtgcgtt ccgtttccta cgatctgccc
     5041 gtcgtcaact actaatatcc ggtctgcgtc aagaactgtg ctaatccttt gcgctaccat
     5101 gatgaccgtt gcatcttcga tgctttcctt cagattctga cggagctgac ggtcagtctt
     5161 catatctaaa gcagaaaaac tatcgtcaaa cacataaatt tccggttttt tcatcatcgc
     5221 tctcgctatc gccatacgct gacgctgccc gccggataag ttcgtgccgc cctgggcgac
     5281 cgaatcgtga tatccttctt tcttcttaca gataaactca tccgcacagg cagtctttgc
     5341 cgcctgtttc cagtcttctt ccgttccgtt ttcatttccg aaattaagat tatctgcaat
     5401 atctcccgag aaaagaacat ttttctgcgg tacgtatccg atcaaagaac gaagatcttc
     5461 cgtgcggtat tctttcgcgt tcactccgtc gatcagcact tctccgaaca tcggatcata
     5521 aagtctgggg atcagtttta atatacttga ttttccccgt cccgttcctc cgatgatcgc
     5581 cgtaacttct cccgaacggg attcaaaact aatatctttc aggatcggct catccgctcc
     5641 cggataagcg aatgtcacat gacgaaactc aaccgttccg cgcaccggac gattttccat
     5701 ctgaaacttt ccaccccgta tcgagctgtc tgtctgcaaa acttccgcga ttcgtttcgc
     5761 acatgcataa cttgtaggaa acatcataat gacaagagac agcatcatca cagatgtaag
     5821 taccatactg atatactggc tgtttgccac taaagaacct acttccatag cgcctgtact
     5881 tacataatat gcgcccatgc ccaatacagc cgccgtcgtc actccgaaga tcacactgat
     5941 agcgggcatc agaaggcttg tgatccgccc tgctgccatt gccgtcatcg cataatcttc
     6001 atttgcctct tcaaaacgtg cattttctgt cttctgctta ttaaacgcgc ggatcacgcg
     6061 tactccttcc aacgattcca aaaacaatct gttgatctga tcgagctttt ttctcagacg
     6121 gatcgaatac ttcgatgcca gtaccatgat gatcgtcaat acagtcagaa gaaccggtat
     6181 cgcgacaaga agaaccgaac tgacttctcc tcccgtagct gcggaaaaca ctagtcctgc
     6241 cgcagccatc ataggcgcaa gcaatccgac tctaagcagc aatgtcaaaa agttctgtac
     6301 attcgtaata tccgaagtgc ttcttgtgac aagactcgct gttccgaacc gatccatctc
     6361 tgccgccgag aactcctgca ctttagaaaa gacctgagtt ctaagctgtg ccgcaaaatc
     6421 tgtagaaata taagatgcaa tcttaacaga gagaaaactg atcacgcagg aaaacaatgt
     6481 tactcccgcc atgatcacgg catacatcca gacaatacgg ttttctcctt ccgccacgcc
     6541 atggctgatc atctgtgcca aaaacgacgg taaaagcatc tgccctactg ccgacagcaa
     6601 tacaagcaaa aacaactgaa cgatctgacc cggccttaac tttacttttg aaaatattgt
     6661 tttcatccct ggtcagcctc ctctcttttc ttatgcggcg ttttctctgc cactttgatc
     6721 ttggacaatc tgaacatcac cgttccaagc gcatatttct gcagatctat atacgcttct
     6781 tccgaaacct tttctccgaa attcataatc tccatttccc cgcttgctcc cgttataaaa
     6841 atatcagacg acgtaaatcc gtttttcaaa taaaattttc tgcgggcaat cctctgttct
     6901 ttgttctctg ccttatcatc aaggcgctcg atcagaagaa caattttttt atctttgtac
     6961 tgcctgcaca cattctgcat aatataactt ccggttcctc tgctgcgaac tttagatgac
     7021 actgccagat aatctgccat taccatatct ttatatggaa taaggacgat aaatcccaac
     7081 acctgttcac cttcggttgc tgccataaca accgcttttt tcgtttttac ggaatgccgc
     7141 agtgcaaaat atggttttct ctcccgcttc ggaaatgctt ccatataaat tgtttttatc
     7201 tctttccact gtttctcctc ggcacatata aactccatcc cgatctcctt tctgcgcttt
     7261 atcattgatt ttcttctttt catcatgtat catataatgt acagtaactg tacagtcaag
     7321 gagtttttta tgaatcgaaa aaattattta tattcttccg gggaatttgc aaaactgacc
     7381 ggagtcaaca aacgcacgct gcactattac aatgaaatcg ggctgttccg ccccgaaatt
     7441 atcggagaga acggatacca ctactataca tgctttcaat ttacagagtt agaactgatc
     7501 cttacattgc gcaagatcgg tctgtccata gacgagatca aagattactc atcccgccct
     7561 tccgacgaat cttttacaga gatgatgaca aaaaagaaaa agctgatcga cgagtccatc
     7621 gaacagcttt tatctgcaca ggctttttta gaacaaaaag cagagagatt aaaaatagga
     7681 atgtattcgc agcacggaac gatcgaacgc tgtacgctgc ccgagagaaa gatcatcttc
     7741 tcatccccta tttcgggaga atacgacgaa gacgattttt ccatcgccgc ggagttctct
     7801 ctccgcctga aaaaattatt tcatctttat gacaatttcg gaagctgtat ttccatagaa
     7861 gatatccaaa aaaacagttt taacagctat cactccttct ttgcatactg cccgcaaact
     7921 tctgacgtat atgatgaaat cctgcctgcc ggaacatatc tgcgcgcttt ttgcatcggt
     7981 gactggggaa aacttcccga aatctaccgc tcgatccttt attatgcccg caaacacaat
     8041 ctgacgctta cagggtatgc atacgaagaa ggactaaatg aaatgtctat caaagaccga
     8101 agtgattaca ttacgatgat cacaattaaa tgtgactgac tgcgatcttg atcacattat
     8161 cttttctatt ttcaaaaata tcatatgcct gttctatttc ttccagcgga aatgtatgtg
     8221 tgataagcgg cgttgtgtcg atacgtccct gctcgatcag tttcaatatc tcatcgcagt
     8281 cacatccgtc tacgcctcct gttttgaaaa taagattttt cccgtacatt tgcggaagcg
     8341 gaagcgtctg gttttcttca tagagcgcca cgactgtgac aatcgcattc gggcgcgcga
     8401 ccttccatgc aagttcaaat gtatccgagc ttcccgccac ttccaaaact cgatccgccc
     8461 cgttatgcct gctgttttct ttcacgaatc gttctgcatc cgtctctttc ggattcacga
     8521 caagtacgtc cggataatgt tcctttacaa atgcgatcct cttcggatct acttcgcata
     8581 aaataatctt ttcgggattc ttaagcatca cgcacaaaag cgtacaaatt cccgtaggtc
     8641 ctcctccgat aatgacgacc gtatcctctt ctccgatctc tgaaatccgc gccgcccaaa
     8701 acccggttgc gaggacgtct cctacaaaaa gagcggcttc atccgtcacc gaatccggaa
     8761 tcttatttaa cccctgctcg gcataaggaa cacggacata ttccgcctgc ccgccgtcga
     8821 tccggcatcc gagcgcccag ccgccgtttt gatccgtaca attattcaca aacccatttt
     8881 tacagaaaaa acattccccg caaaatgttt cgacgtttac agtgacacga tctccgggtt
     8941 ttacctttgt cacacgcctt cctgtctctt ccacgatccc gaccatctca tgaccgaccg
     9001 ttatccccgg aactgctctc ggaacggcgc cgtgtttgat atggagatcg ctggaacaaa
     9061 tacttgccaa agtcacacgt acgatcgcgt cgtcctcact ttgcagcgtt ggtttttctt
     9121 tctctttcaa aacaaacttt ccgttttctt catatgtgta tgctaacatt tctcctgtct
     9181 gctcctcttt cctatgctat tcttatcatg cttatgctaa cgtcataaat gtaaggacga
     9241 ttgccagaat ccagtttaca acggctatac atattcccgc gatacttaag acttttcctg
     9301 cctttgccat ggctgttcct gacagatcat ttcttttccg cactcttaca agcgatacag
     9361 tccccagaac gatcccgacg atcggaatcc aaagtgcgaa aataacgctc acaataccga
     9421 gcacaagcaa cgagatgcct gtgtcgtttc ccgttttatc cgatgccgga tttttctcac
     9481 gtttcgccat tgctttcctc caatatcata ttacggctca tatatacata tgaaccgtaa
     9541 tattttttca ttcattctat tttctttttc acaaactttt ctgccgtcgt ctgttcccca
     9601 acaaaacgac cgcaaatgaa tcggcagaac actgcctgca cgatacgatc cagacattac
     9661 gcgatctcat accagatgat ctggttcggc tcaagagcaa gctcaaagct gcttccgtct
     9721 ccgcggtaga tcgttgtctg ctgcggctca tccgtattat ttacaacaca gtatttgttg
     9781 ttcttcggat aagcatgtac atctacatta taatttgtac tgaaccatct gttcagttca
     9841 tcttcgctgt gtgtgctcca aagcactgca cggtaaagta cacggctgtt ctcgaagctg
     9901 tacggaagtc cgctgatata aacagcgcgt ccttttccga attcgtttac tgccatctgt
     9961 acttcttttt ctttctgcac aaggatcgtt gcactcggaa gagcataaat attcttcttg
    10021 ccttctccga aatctacttc ttcagaatct tccaaaatga agtgttcatg ctcatcccag
    10081 ttgtatttgt cgtatccgag tgtgaagccc gtttcttcct ctacaccgaa tacgttagca
    10141 agctggaaga aatgtccctg atactgatga ccggaaggtt cgccgacacc gataattcct
    10201 ccgccgttgt aaacgaatcc gcggatcgct tcgatgatct tcgggttttc ccaccattct
    10261 cctcctgtat gcgccgtatc tgcatccccg acattgatga tcacatcgat atcgtcaagg
    10321 atcgccggat tatccagaat atcctggaag ttgataaata caacgtcaaa cggcgctcct
    10381 gacagcgact cgatgattcc tgcatatgaa tagttctgtt tctgatacag tgcatgatgt
    10441 accatatggc atccccatgc tctcatcttg ccccagcagt taagcactgc gactttcttc
    10501 acgcagtacg gtgtcgttcc ttttacattt gcataaagcg tacggaactc gttgcatacc
    10561 tgctctacat aatcaaggaa ttccgggaac tgaagcgcaa gtttaagata accgccgtat
    10621 ccgatacgat cgatcggttt tctaaggatt gctcttcttg ctgtcaccca gttatatttt
    10681 gcttctttga ccggatctcc gttctcgttg aacacatccg ggaagaagta cggcaacaga
    10741 cgtccttctg tatatttcac tccttcgata tcactgatca gacgaagcgt cgaaccgttt
    10801 cctacgcttc ctacaacagc gtcaatacca agtgttttaa actcttccat aaacggctct
    10861 gttccgatcc agtgatctcc gaggaacatc attgcttttt taccacattc gtgcgtgatc
    10921 tctgtcatct cttttacgat cttggcaact tctcttctct ggaacgcctg aaaatcctga
    10981 aattctttgc tcggaacacg atactgatta ttgtaatatc cctgatcgat aatatattcc
    11041 gcgcggaacg gatatcccac ttcttcttca aactgtttta agatatacgg acttaccgac
    11101 gccgaatatc cgtaccagtc tacatatttc tcacgggcaa gctcgtcaaa tacaagtgta
    11161 aactgatgga aaaatgttgt aaaacgaagt acgtttacat acggatgatc ctcaataaat
    11221 tttctcagac gtttcaatgt atactcatgt gtcttcggct gacggacatc aaaggtgatc
    11281 tgatgctcta catctttcca gtcatttgtt acggcattgt acatatgcac cggatcccac
    11341 atgatatagg cgaggaagct cacagtatac tcatggaacg gaactgcctt gatcgtaacc
    11401 tttccttcct cttcattata atcccactcg tctgtcggaa cgacttttcc tgtcgtgcgg
    11461 tctacaacct cccaccaacg cttaatgtca tcccgtgtat tcggactgag catgtcagga
    11521 taaagacctt tcatgatctc aatcgacaga ctctcggaat cagccgtata aaacggcgtc
    11581 ataagataca tctgctgtat ctcttccgga tttgccttcg cccactcatt gtcttttctt
    11641 gtcgtataat atgtagaata aatttccgcg tctgcgtctt tgagcgcctc cggaaaatct
    11701 gtaccgtcac agtctctgat cgcatctgct ccccagcgct tcatcagttc caaagtctcg
    11761 ggaacaacgt cgagatctgt agggatggtc acaagtcctt ttgaattttc catgaattat
    11821 accctctttg ttctttcctg ttttcttttt ctgttttctg ttatattttg ctatttatat
    11881 ttatgattat aaaaagcaag aagtccgatc agacccgcaa gtcctgcaag cattgtcaga
    11941 agcccctgcg gtccgatatt tttctgaccg ataatgataa gggctacaga cgcgataaaa
    12001 ataacagcaa caatgattcc tactaaaacc tgttttgttc tttcatccat aatatcaacc
    12061 tcctacccct tcaatccgcc gagtgtcata ccctgtgtca gtttcttctg tacacagata
    12121 tacaaaatca acgtcggcag cataacgata acaagacctg catacatctg accgtattga
    12181 actgcagatt tctgcgccgc catcaagttc ataagtccta ccggcagagt tttcagttcc
    12241 ggttttgtca gaagtgtcat tgaaataatg tattcattcc agaatgacag gaaactgaac
    12301 aagattaccg taacaatact cggttttgcc atcgggaaaa taatctttac catcgttgta
    12361 aagtaacttg ctccgtctac ataagcggct tcttcaaaat cttttgccaa agatctgaaa
    12421 tatccggaca aaagatagat cgtaaacgga agcgccgtag acgcatacac aagagccagc
    12481 acaaacaggt tgttcaaaaa gaacgtacct cctgtgagct gacgaagcgt tttatcccag
    12541 ttattgagca taaggaagat cggtacaaca atgtaactta cgttaatgaa caggcctgcc
    12601 ataaaaagta cgttccagaa ttttgacgtt ttaaacttga atctggacaa tacatatgcc
    12661 gccggaagag agatcacgat cagcaaaagc aatgcaagcg ctgttacgat cactgagttg
    12721 agcatgtaag ttcccatgct cgccttctcc cacgcatcga taaagttctg gaaataaatc
    12781 ttctccggaa gtacccacgg acttccgtaa aactctgaat tctgcttaac agatgcaaca
    12841 aatacccagc cgaccggaat gataattgtg atcgcaagga gaattaataa gatatatact
    12901 accacttttg aaagtgtcaa tggttttttt gactctttta cttttgtttt tcccatcttg
    12961 atctccttcc cttaaaattc gatttcctga cgttttgttg ccacattcag aattcccgca
    13021 agtgcaaacg agaagaggaa taccataaca ccgatcgcca taccatatcc ataggaagag
    13081 ttcgtgtacg cttcctgata catataactt aagaatacat ttgacgctcc gtccggtccg
    13141 ccgtttgtca ttgcttttac aagcaggaaa ctcatattga tcgtactgat aacgaagaat
    13201 gtaagtgttg ttctgatgtt tgtccagatc agcggaattg tgatcgtaaa gaactgtctg
    13261 atctttcccg cgccttccaa agccgccgac tcataaaggc tttccggcac gtttgccata
    13321 cttgccatat acataaccat atagtaaccg attgcctgcc atacaagtgc aatcacaata
    13381 ctgtaaataa ctatcttctg atctccaagc caaagcgtcg ggttttccgc tcctttgaac
    13441 agattcaaaa aagagttcaa aagtccaagg ttcggatcat agattgccga gaaaatagca
    13501 ctgataacta ctaccgacaa aatgttcgga atgtagaaaa taacactgaa aaatgttttt
    13561 cctttaattt tttcccgtga caaaatataa gcaaatacaa gcgcaaatgt aaatgttaca
    13621 agtgtaacaa gaacgatcag caaaatcgag ttctggaacg cctgataaaa tttgtcactc
    13681 ttgaaaagta ttttaaagtt gtccaatcct acaaatgtct tattatctgt atatccaccc
    13741 catttaaaca tagacatacg gaaaatatcg atcgtaggca ggatcataaa tacgataaac
    13801 aatatcgctg ccggggcaag acagcaaaat acaaacagcc cttttccttt tcttgctttc
    13861 attaattcat ctcctgtcag gataaaacgg gcggtgaaaa cagattcacc gcccgcctac
    13921 acctgtgata ttcttccggt catcagaacc ggtaaatttc cggtgattta tttttttttt
    13981 atttcagcgc ttcatggaat ttagcactgt cagctttgat ctgatcgatc caatcctgct
    14041 ctgtcttgtc tccagttaca agagagttaa ccggatcaaa gaatgtagaa cgtgttgtaa
    14101 gaccttcgat cggctctgtt gctgcgaatg catccataac tgcaactgcg cctgtgtcgt
    14161 agattgagta atacagtttt gtattatcat ctgtgatcat atcagacatt ccttcgatcg
    14221 gctgaactgc tccgccttta tcgaagaaga tcttagctgc ttcatcagag tacaggtaag
    14281 cgataaattc ttttccgaga tcctgctctt ttgcttctgc cggcatccag atctgctcga
    14341 agaatgtgta agaagctctc tctccgcctt ctttaacagc cggaagagct gtgaatcccc
    14401 actcgaatcc gtctgctctc ggagcatctt ccatctcgcc gattacccag tttccgttcg
    14461 gcatgaacag cgctttgttg tcaagtacaa gctgttggtt cttacggaaa tcgttgtcgt
    14521 ttgcattagc cggtgttgtt ttttctgtat atgtagcaag ttttgcaaca atgtcaaatg
    14581 cctgtttagc gccttctgta tcccagatac catcttcata tgtcagcgct ttagagaaat
    14641 cgtctcccat tgtctcatgc atcaaagcat agaagaatgc gtcaaagtaa cctgttgtcg
    14701 gatatgtaaa caatgcgatt ccttcttctt tcgctttgtc gccaagtgcc cacatctcat
    14761 cccatgtagt cggaacatcc cagccttttt cctcaaacag tcctgcattg tagaacagtc
    14821 cgcatgggcc gtagaacatc ggcatcagat atgtttttcc gtctccgtac ggatttgtaa
    14881 ttgtattctc aacgaatccc ggaaggatct tgtctcctac agttacttct tcgcccggta
    14941 ctgtcatatc aagaacatct gtcagatcaa caaggttgtt gtcttttacg aatgtctctg
    15001 tgattcccga ttcagctcct acagctctta agatcacgtc cggatactcg ccggatttca
    15061 tctgcgggtt gattacatct tcgagctttt tgtcgattgt aagttcaacc ttaacgcctt
    15121 cgtgagattt ctcgaatgct gagcaaactt ctttccacat gtctgcgccg taagctgttt
    15181 ctaccgctgc aaccttcagt gttttttcgt ctccaccttt gtcagcgtct ttgtcgtctc
    15241 cgcctgatga accacaagct gcaaggctga atgccataga agcaacaagt aacagactga
    15301 tcagtttttt ctttttcata atgtcaaaat ctgcttcatc taaatgaacg ccgtggaa
//
LOCUS       DBMDXF010000022        15276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1151_length_15276_cov_1.901680, whole genome shotgun sequence.
ACCESSION   DBMDXF010000022 DBMDXF010000000
VERSION     DBMDXF010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 15276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15276
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1151_length_15276_cov_1.901680"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="ACZPD4_01800"
     CDS             complement(<1..222)
                     /locus_tag="ACZPD4_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1377846.1"
                     /translation="MDIRGKKVLVFGSGISGEGSCRLLLEKGADVVLYDGNEKLCADE
                     IEAKILETKKENGQFSIVLGTFPEEIIEEL"
     gene            complement(225..1181)
                     /gene="mraY"
                     /locus_tag="ACZPD4_01805"
     CDS             complement(225..1181)
                     /gene="mraY"
                     /locus_tag="ACZPD4_01805"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1377847.1"
                     /translation="MSSHVVIPVLAAFAVSLVLGPIVIPFLRKLKMKQTERVEGVQSH
                     LKKAGTPTMGGIIILAAVIVTSLFYVKDYPKIIPVLFLTVAFGLIGFLDDYLKVVLKR
                     SDGLMPMQKMALQIIVTAVFAFYLVKVADIPLTMLVPFSNGYYLDIGWMAVPLLFVAV
                     IGTVNGTNFTDGLDGLASSVTVLVATFFTVVAIGTKSGIEPVTCAVVGALLGFLLFNV
                     YPASVFMGDTGSLALGGFVAGTAYMMQMPLFILIVGIIYLVEVASVMIQVTYFKKTGG
                     KRFFKMAPIHHHFELCGWSETRVVAVFSIITALLCLIALMGV"
     gene            complement(1229..2950)
                     /locus_tag="ACZPD4_01810"
     CDS             complement(1229..2950)
                     /locus_tag="ACZPD4_01810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1377848.1"
                     /translation="MKNKTYNKRKVLVVFLCAAFVLFCLIGRLVYLMIFDAEYYQNMA
                     EELHEREREIKAARGEIVDRNGVVLATNRTVCTISVIHSQIKDPQQTADMLSKELELD
                     REDVLKKVEKVSSMEKIKTNVDKKTGDRIREYNLPGVKVDEDFKRYYPYDRLASRVLG
                     FTGSDNQGIIGLEVKYEDVLKGTNGKILTVTDARGIELEGVAEDRIEPVAGNTLKTSL
                     DYNIQSYCEQAAEKVLEEKQAEGVSVLLMNPQNGEIYAMVNVPEFNLNEPYRLNDKTE
                     DQMTDEERQNRLNQMWRNRCINDTYEPGSTFKIITSAACLEEGVVSLDDTFSCPGYRI
                     VEDRKIRCHKVGGHGTETFVQGIENSCNPVFMDIGLRLGSERFCDYFEQFGLMSLTNI
                     DLPGEAGTIMHKREDIKAVELATMTFGQSFQITPIQMAVTVSSMVNGGRRVTPHLGVS
                     VLDKKGKTVKTFKYGEKEGIVSEKTSETMQELLEKVVSEGSGKNAYLEGYSIGGKTAT
                     SQTLPRSAGKYISSFIGFAPADDPQILGMVIIHDPQGIYYGGTIAAPVLRDIYDNVLP
                     YLGIEKK"
     gene            complement(3037..5043)
                     /locus_tag="ACZPD4_01815"
     CDS             complement(3037..5043)
                     /locus_tag="ACZPD4_01815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1377849.1"
                     /translation="MAVKKRKKSRFEFLTRKFPLRMQRKLVMLFMAVVLAFVVLIGRI
                     TYINVTKGSKYTKVVLDQQNYDSRVIAFKRGDIVDRNGTKMATSERVYNVILDVAVMM
                     DKKEYIEPTKEVLKECFDVEGDVVDKLIEESPDSRYEVLVSDVDYATAQKFKKIDEDD
                     KKYPNVKGIWLEEDYLRSYPYGSMASDVLGFVYGGNQGALGIESAYNSILNGTDGREY
                     GYFDSDSSVERTVKPAKNGNTVVSTIDVTLQNIVEKCILEFNSAHAADGNPGSKNTAV
                     IIMNPNTGEILAEASYPNFDLNDPRNLSLLYTKEQWDAMSEEDQVEAMNDLWRNFCVS
                     DAYEPGSTAKPFTVAAGLETGTLTGNESYYCGGSKDVLGTKISCHYKSGHGQETIQDA
                     IANSCNVALMDMAAVIGPENFTKYQHIFGFGEYTGIDLPGEAETSGLLYTADTMGEID
                     LATNSFGQNFNVTMTQMVSAFSSLVNGGYYYEPHVVKQIQDEDGNVIETKDPVLLRKT
                     VSSETSKMLRQYLKATMDYGTGTRAKVEGYDIGAKTGTAEKLPRGNGKYLLSYIGYAP
                     QNNPEVVVYVVIDEPNTAVQDDSSLVLGLSQKIMSEAFPYLGITTIAQSGQNAQAQGQ
                     NFGNTEYTDYDENYEDTYGNADGSYVDENYTPDLDDWASSDVSE"
     gene            complement(5054..5605)
                     /locus_tag="ACZPD4_01820"
     CDS             complement(5054..5605)
                     /locus_tag="ACZPD4_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377850.1"
                     /translation="MAANYRTSKYRTTSLSERDKSGFYVYGNTVRQAETLPRKTKSPY
                     SQQREQTKRTSSRVVKNRNRAMSISPAYAVFLTAAAVCAVLICVLYLNLQSEVVSRSE
                     NVTAMQEQLADLTEANNTAYNSAADSVNLETVRDRAMNEMGMVYAAQGTIVEYDSPEG
                     DYVKQYNDIPESGVLAKSSDVLK"
     gene            complement(5691..6626)
                     /gene="rsmH"
                     /locus_tag="ACZPD4_01825"
     CDS             complement(5691..6626)
                     /gene="rsmH"
                     /locus_tag="ACZPD4_01825"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1377851.1"
                     /translation="MAFEHVSVLLNETVDGLNVKPDGVYVDATLGGGGHAYEVCRRLG
                     SKGRFIGIDQDADAIKAAGERLKGFGEKVTIIRSNYRDMKPQLQNIGVDKVDGIVIDL
                     GVSSYQLDTAERGFSYRVDAPLDMRMDQRQKMTARDIVNEYSESELYRVIRDYGEDKF
                     AKNIAKHIVAERRKNPIETTGQLNEIISHAIPMKFRKTSGHPSKRTFQAIRIELNHEL
                     DVLRESLDDMIEMLNPGGRICIITFHSLEDRIVKSAFRKNENPCTCPSHFPVCVCGKI
                     SKGKVITRKPILPSEAETESNSRSKSAKLRIFERA"
     gene            complement(6640..7077)
                     /gene="mraZ"
                     /locus_tag="ACZPD4_01830"
     CDS             complement(6640..7077)
                     /gene="mraZ"
                     /locus_tag="ACZPD4_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1377852.1"
                     /translation="MLLGEFNHSIDEKGRLIIPAKLRDDLGDSFVICNGLEGCLFVYS
                     QEEWNKFVAELETLPRMSKDARIFKRYFFGSASEGSFDKQGRVLVPPSLRKAAGLEKD
                     VVLVGVQDRIEIWDKALWEERSQISEEDLDAIAERMESIGIRI"
     gene            complement(7401..7652)
                     /locus_tag="ACZPD4_01835"
     CDS             complement(7401..7652)
                     /locus_tag="ACZPD4_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377853.1"
                     /translation="MSQEKVDRYKQEKVNRKQIMRKQKLMNNMRKGVLVVVALALIGW
                     LGYSAYDIYESGKERTVSEVNYDAVSDYLNGLNAEGSAE"
     gene            complement(7712..8557)
                     /locus_tag="ACZPD4_01840"
     CDS             complement(7712..8557)
                     /locus_tag="ACZPD4_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1377854.1"
                     /translation="MDYKIVLDSCGELTYKMKESGIYHTAVLSMLVDGDTILDDESFD
                     RKDFLKRVAVSPECPKSSCPSPDAYMELYGGAEKRVYVVTLSAELSGSYNSAQLGKEL
                     WKEENEDGDKQIYVFNSRSASVGESLIALKIQECEEKGYSFEEVIEHVEAYIEEQHTY
                     FVLENLDTLRKNGRLTGIKSLMASALNIKPVMGSTPEGTICQLGQARGVKKAIAKMAE
                     HIVADGKNTENKVLAIAHCNCPERAREIERMILDKIKVKDSFIVETGGISTMYANDGG
                     IIVVL"
     gene            complement(8663..10030)
                     /locus_tag="ACZPD4_01845"
     CDS             complement(8663..10030)
                     /locus_tag="ACZPD4_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1377855.1"
                     /translation="MPMRPVREDVYDTAPMRPMNIRSGYEGYRRTIDRRREVIRTERP
                     VSGRVNSRKGKKERTSGRTRYENRNSLSALIARQRSAAGKEQYFDYDLLFVIIFLMCF
                     GLVMLYSVSFYEAQADFGNDMYYFSKQALIGVGGFIGMYLVSKLDYHLYGAFAFEIYV
                     ISMFLMALVQTPLGVTVNGARRWIGLPGNLSLQPAEITKIAVILFISYELCRLGKRAY
                     SPKGIAQILAFGAVASAGVLFLTDNLSTAIIVAGITCILIFVSHPKTKPFLVIIGIGI
                     AVAAVGIAILSVTVANSDNFRLQRVISWLNPEATADTGSFQVMQGLYAIGSGGLFGKG
                     LGNSTQKLGVIPEAQNDMILVVICEELGVFGAVVILVLFALLLYRLIFIAKNAPDLFG
                     SLIATGIFAHIALQVILNIAVVTGLLPTTGITLPFISYGGTAIVFLMAEMGIALGISR
                     KIRLE"
     gene            complement(10143..12026)
                     /gene="asnB"
                     /locus_tag="ACZPD4_01850"
     CDS             complement(10143..12026)
                     /gene="asnB"
                     /locus_tag="ACZPD4_01850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1377856.1"
                     /translation="MCGFAGFVGKVEDRETVLVNMMDTIIHRGPDSAGKYVDEDAALG
                     FRRLSIIDLSSVGDQPLYNEDKSMVLVFNGEIYNYQELRKELIEAGHVFVSNTDSETL
                     IHGYEQWGEKLVDRLRGMYAFVIWDTKKKRLFGARDIFGIKPFYYAQMNQTFLFASEI
                     KAFMEHPKFDKIFNEDALGNYLSFQFVPTNETFFKGVFCLQPGHYFIYEDGKMEISRY
                     FEPNFTGKYEKTFDEAAAEVEKVMKESVEKHKISDVEVASYLSSGVDSSYLTYLGQVD
                     HTFTVGFDEGEYSEIQDAKDFAESIHMKNDAKVITPDEYWDSLSDIQYYMDEPVADPA
                     AVALYFLSKEAAKKVKVVLSGEGSDELFGGYNIYCEPLEHTAFNKIPMPIRRFMGKFA
                     EYCLPRGMKGRGFLMRHGKTLEERYFANATNIFTEREAAKILKKGCRPGIQDVTKPLY
                     NRVKDKDAVTKMQYVDLHLWLVHDILMKGDKMGMANSLEVRVPFLDRNVLELAESLPL
                     QYKVQAPRTKVALRAAAERSIRNKTAEKKKLGFPIPIRVWLKEEKYYNRVRAMFASSM
                     AKKFFNTDLLMKMLEDHRDGKNTNEKTDDSRKIWTVYIFLVWYDRFFEHGKPVHPSMT
                     AGK"
     gene            complement(12256..12330)
                     /locus_tag="ACZPD4_01855"
     tRNA            complement(12256..12330)
                     /locus_tag="ACZPD4_01855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12293..12295),aa:Pro,seq:ggg)
     gene            12452..12715
                     /locus_tag="ACZPD4_01860"
     CDS             12452..12715
                     /locus_tag="ACZPD4_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HIR1377857.1"
                     /translation="MATCQTNNQMRYMRRTMPDTSCPCHETVPMNGCPNTRDFFPANM
                     PIAMAYVPWQKWQNIYEPCRALKNGTIFEELNKPFSGKGACCR"
     gene            12793..13071
                     /locus_tag="ACZPD4_01865"
     CDS             12793..13071
                     /locus_tag="ACZPD4_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HIR1377858.1"
                     /translation="MKNMHRSMNRNQLMEHINQVSFAVDEVKLYLDTHPCDSEALAYF
                     HEMSAHRNDALKQYASAYGPLTVDTADYSCAERWNWINEPWPWQEGGC"
     gene            13075..13671
                     /locus_tag="ACZPD4_01870"
     CDS             13075..13671
                     /locus_tag="ACZPD4_01870"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIR1377859.1"
                     /translation="MWNYEKRLQYPVKITQTNPKIAKIIISQFGGPDGELAASMRYLS
                     QRYTMPYKEVTGTLTDIGTEELAHMEMVCAIVYQLTKDLSPEEIERSGFAPYYVDHTL
                     ALWPQAASGAPWTATYFQSKGDPITDLHEDLAAEQKARTTYDNILRLVKDPEVCDPIR
                     FLRQREIVHYQRFGESLRIVQEKLDSKNFYAVNPEFDK"
     gene            complement(13777..15138)
                     /locus_tag="ACZPD4_01875"
     CDS             complement(13777..15138)
                     /locus_tag="ACZPD4_01875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1377860.1"
                     /translation="MDNQYNYYNPNENSNNEDRQRMNSQSEGSQNGEQKGNTPQWQPG
                     GDHLKKEKKPHKNLMRTVAVVGFAILFGVVSSGIFLTTTVVGNRVFGLLDSSKSTTAS
                     SDKVSKGTPLSKSSSVVTSDVSEVVDQVMPSIVSITSMSVEEVRSFFGGTYEQQSKGA
                     GTGIIIGKTDTELLIVTNNHVVAGSDTLTVAFTDESTVEANIKGTDAQYDVAVIAVPI
                     ENISDDTLDAISVATLGDSTSLKVGEPAIAIGNALGYGQSVTTGVISALNRSVSMTNE
                     ETGDTQESGVKLIQTDAAINEGNSGGALVNVNGEVIGINSSKLVGDSVEGIGYAIPIS
                     DVGDLIENLMNRETKTKVAEDKRGLIGITGISVSDAFSQQIEMPAGVYVTEIAKGGGA
                     EKAGMTKGCIITAIDDITVDSMEALQEQLQYYEKGTTVTLKIQIPQTNGEYKEQTIEV
                     TLQ"
BASE COUNT         4107 a         4098 c         2524 g         4547 t
ORIGIN      
        1 aagctcttct ataatctctt ccggaaatgt tccgagcact atactgaatt gcccgttttc
       61 ttttttagtt tctaaaattt tagcttcaat ctcatcggcg cacagttttt catttccatc
      121 atacaaaaca acatccgcgc ctttttcgag caaaagcctg catgatcctt ctccactgat
      181 acccgaaccg aagacgagca ctttttttcc tcttatatcc atttttatac ccccatcagc
      241 gcaatgaggc aaagcaacgc cgttatgata gaaaatacag ctaccactct cgtttcagac
      301 catccgcata attcaaaatg atggtggatc ggcgccattt taaaaaacct ctttccgcct
      361 gttttcttaa aataagtaac ctgaatcatg accgaagcca cttccacaag gtaaataatt
      421 ccaacgatca ggataaacaa aggcatctgc atcatataag ccgtgcctgc cacaaatcct
      481 ccaagagcca aagaccctgt atctcccata aacacacttg ccggatatac gttaaacagt
      541 aaaaatccaa gcagcgcccc gactacagca caggtgacag gttcgatccc gcttttcgtt
      601 ccgatcgcca caactgtaaa aaatgttgcg acaagaacgg tcacactgga agcaaggcca
      661 tccagtccat ctgtaaagtt tgttccgttg accgttccga tcactgccac aaacaaaagc
      721 ggaactgcca tccagccgat atcaagataa tatccgttag agaacggcac aagcatcgta
      781 agcggaatat ccgccacttt cacgagatag aatgcaaaca cagccgttac aataatctgc
      841 agagccattt tctgcatcgg catcagtcca tccgaacgtt tcaacacaac tttaagataa
      901 tcatcaagaa atccgatcaa accaaacgcc actgtaagga ataaaaccgg aatgatcttc
      961 ggataatctt tcacatagaa cagggatgtc acaataacag ccgcaagaat aataatgccg
     1021 cccatcgtcg gagttcccgc ttttttcaaa tgagactgaa cgccttctac acgctccgtc
     1081 tgcttcattt tcagtttccg caaaaaagga atcacaatcg gtccgagcac aagacttacc
     1141 gcaaatgccg caagaaccgg aatcactaca tgactactca taaatccacc tcttcatctc
     1201 ttttatctca taaagtataa accatccatt actttttttc aatcccgaga taggggagca
     1261 cattatcata tatgtcccga agcaccgggg cagcgatcgt tccgccgtaa taaattccct
     1321 gaggatcatg gatgatgacc attccgagaa tctgaggatc gtccgccgga gcaaatccta
     1381 taaacgaaga aatatatttt ccggcgcttc ttggaagcgt ttgagacgtc gccgtctttc
     1441 ccccgataga atatccttca agatacgcgt tttttcccga tccttcggaa acaactttct
     1501 ccagaagttc ttgcattgtc tccgatgttt tctccgaaac aatcccttct ttttccccat
     1561 acttaaacgt cttgaccgtt ttccctttct tatctaagac cgacacgcca agatgcggcg
     1621 ttacacgcct gccgccattt accatcgaac tgaccgtcac cgccatctgg atcggcgtaa
     1681 tctgaaatga ttgaccaaac gtcattgtgg ccagttctac cgctttaata tcttctcttt
     1741 tatgcataat cgtccccgct tctccgggaa gatcgatgtt cgtcaggctc atcagaccaa
     1801 actgttcaaa atagtcgcaa aacctctctg atcccagtct aagtccgata tccataaata
     1861 ccggattaca cgaattttca atcccttgca caaaagtttc cgttccatgc cctcctactt
     1921 tatgacaccg gattttcctg tcttctacga tccgataccc cgggcaggag aaagtatcgt
     1981 ctaaagagac gacgccttct tcaagacaag ccgccgacgt aatgatctta aaagtagatc
     2041 ccggctcata cgtatcgtta atgcagcggt ttctccacat ctgattcagt ctgttctgac
     2101 gttcttcatc tgtcatctga tcttccgttt tgtcattcaa acgataaggt tcattcaaat
     2161 taaattcagg cacatttacc atagcataaa tttctccgtt ctgcggattc atcagcaaga
     2221 ccgatacccc ttctgcctgc ttttcctcca gcaccttttc ggccgcctgc tcacaatacg
     2281 actggatatt gtaatcaaga cttgttttta atgtatttcc ggcaaccggt tcaatccggt
     2341 cctccgcaac cccttccaac tctattcctc tggcatctgt aaccgtcagt atcttcccat
     2401 ttgttccttt taatacgtct tcatatttca cctcaagacc gatgatcccc tgattgtcgc
     2461 tccctgtaaa tccaagcact cttgacgcaa gccggtcata tgggtagtat cttttgaaat
     2521 cttcatccac ttttactccc ggcagattat actcacggat acggtcgcct gtctttttgt
     2581 caacattcgt ctttattttt tccatagaag atactttctc tacctttttc aaaacatcct
     2641 ccctgtcaag ttccaactct ttactgagca tgtctgccgt ctgctgcgga tcttttatct
     2701 gactgtgaat aaccgaaatc gtacacaccg tcctgtttgt cgcaagaaca actccgtttc
     2761 tgtctacgat ctcccctctg gccgctttga tctccctctc cctttcatgc agttcctccg
     2821 ccatgttctg ataatactcc gcatcaaata tcataagata aacaagtctg cctatcagac
     2881 aaaaaagcac gaaagccgca cataaaaaaa cgacaagtac ttttctctta ttataagttt
     2941 tattttttat tctttcctct attttgtttc tcataacaaa aaatccccac agaagatatt
     3001 tttacagttt attatatctt ctgcagggtt attcctctac tctgaaacgt cagacgacgc
     3061 ccaatcatct aaatcaggtg tatagttttc atccacatac gatccgtctg catttccgta
     3121 tgtatcctca taattttcat cataatccgt atattctgta ttgccgaaat tctgaccttg
     3181 cgcctgagcg ttttgcccgc tttgtgcaat agtcgtaatg ccaagatacg ggaatgcctc
     3241 agacatgatc ttctgactta atcccagaac aagggagctg tcatcctgta cagccgtatt
     3301 aggctcatcg atcacaacat aaactacgac ttccggattg ttctgcggcg cataaccgat
     3361 ataagataac agatactttc cgtttcccct cggaagtttt tccgctgttc ccgttttagc
     3421 gccgatatca tacccttcca cctttgccct cgttcctgtt ccgtaatcca tcgtcgcttt
     3481 caaatactgt ctcaacatct tagacgtttc ggaagacact gtctttctca aaagaaccgg
     3541 atctttcgtc tctattacat tgccgtcttc atcctgaatc tgttttacaa catggggttc
     3601 ataataatat cctccgttca caagagaaga aaatgcagaa accatctgtg tcatcgttac
     3661 attaaaattc tgtccgaatg aattggtagc cagatcgatc tctcccatcg tatccgccgt
     3721 ataaagcagt cctgacgttt ccgcctctcc cggaagatca atccctgtat actctccaaa
     3781 cccaaaaata tgctgatatt ttgtaaaatt ttccggaccg atcaccgctg ccatatccat
     3841 aagagctaca ttacatgaat ttgcaatggc atcctgtatc gtttcctgtc cgtgtcccga
     3901 cttatagtga cagctgatct tcgttccgag aacgtcttta gaacctccgc agtaatagct
     3961 ttcgtttccg gtcaatgttc ctgtttccaa gccggccgcc acagtaaacg gtttcgcagt
     4021 agaccccggc tcataagcgt cacttacgca gaaatttctc caaagatcat tcatcgcttc
     4081 gacctgatct tcttcactca tcgcatccca ctgctctttt gtataaagca aagatagatt
     4141 tctcggatca ttcaaatcaa aattagggta cgatgcttcc gccagtattt ctcctgtgtt
     4201 cggattcata atgataacgg ctgtgttttt actgccggga tttccatctg cggcatgcgc
     4261 actgttaaac tctaaaatgc atttttccac aatattttgc aatgtcacat caatggtaga
     4321 cacgaccgta tttccgttct tcgcaggttt gacagtacgc tctaccgaag aatcggagtc
     4381 aaaataccca tactctcttc catccgttcc gttgagaatt gaattatacg cgctttcgat
     4441 tccaagcgcc ccttgatttc ctccatatac aaatccaagc acatcactcg ccatagaacc
     4501 atatggataa ctcctcaaat agtcttcttc cagccagatt cctttcacat tcggatattt
     4561 tttatcatct tcatcaatct tcttaaactt ctgagcagtc gcataatcca catctgacac
     4621 aagcacttca taacgactgt ccggagactc ttcgatcaat ttatcaacaa cgtccccctc
     4681 tacatcgaaa cattctttca atacttcctt tgtcggttcg atatactcct ttttgtccat
     4741 cataaccgcc acgtcaagga tcacgttata tacgcgctca cttgtcgcca tctttgttcc
     4801 gttacgatcg acaatatcgc cccttttaaa agcgatcacc ctgctgtcat agttttgctg
     4861 atctaaaaca acttttgtat atttgcttcc cttcgttaca tttatatagg ttattcttcc
     4921 tataagaaca acaaaagcca gtactactgc cataaatagc attaccagct tccgttgcat
     4981 tctgagcggg aattttcttg tcaaaaattc aaatctactc ttttttcttt tctttacagc
     5041 cataattttt tccttacttt aatacatcgc tggactttgc gagcacaccg ctttccggaa
     5101 tatcgttata ctgctttaca taatcccctt ccggactatc gtattctacg attgttccct
     5161 gagcagcata aaccatcccc atttcattca tcgctctgtc ccgcaccgtc tcgagattca
     5221 ccgagtctgc cgccgaatta tatgcagtat tatttgcctc cgtcagatct gcaagttgtt
     5281 cctgcattgc cgtaacattt tccgatctgc ttacgacctc cgactgaaga tttaagtata
     5341 aaacgcaaat caataccgca cagactgccg ctgccgtaag aaatacagca taagccggac
     5401 taatactcat cgcgcggttt cggttcttaa caacccggct gcttgtccgt ttcgtctgct
     5461 ccctctgctg cgaatacgga ctcttcgttt ttctcggaag agtttctgcc tgacgtacag
     5521 tatttccata tacataaaat ccacttttgt ctctttcact tagacttgtc gttctgtatt
     5581 tggatgtacg atagtttgct gccatgacaa atgccccttc cttttttgta ttttcctaag
     5641 ggttctcccc tcccctcgga aattcgtttc tataatgata cactcccaat ttatgcccgt
     5701 tcaaatattc tgagtttcgc actttttgaa cggctgtttg attccgtttc tgcctccgac
     5761 ggcaaaattg gttttctcgt gatcactttc ccttttgaaa tcttcccaca gacacataca
     5821 ggaaaatgac tcggacaagt gcatggattt tcgtttttcc taaatgcact tttcacaatg
     5881 cggtcttcta acgaatgaaa tgtaataatg catatccttc cacccggatt cagcatctcg
     5941 atcatgtcat ccaacgactc cctcaaaaca tccagttcgt gattcaactc gatccgaatc
     6001 gcctgaaatg tccttttcga cggatgacct gacgtcttac gaaacttcat cggtatcgca
     6061 tgactgataa tttcattcag ttgtcccgtt gtttcaatcg gatttttcct acgctccgcc
     6121 acaatatgct ttgcaatatt cttagcaaat ttatcttccc catagtcgcg gatcacacgg
     6181 taaagttccg actcactgta ttcgttgaca atgtctctcg ccgtcatttt ctgtctctga
     6241 tccattctca tatcaagcgg cgcatccacc cgataagaaa atccgcgttc ggcggtatca
     6301 agctggtatg atgaaactcc cagatcgatc acaataccat ctactttgtc aacacctata
     6361 ttctggagct gcggcttcat atcacgataa ttgctccgaa ttatcgtaac cttctccccg
     6421 aagcctttca ggcgttctcc cgctgcttta attgcatcgg catcctgatc tattcctata
     6481 aatctcccct tactgccaag ccttctgcac acttcatagg catgtccgcc tccgccaagc
     6541 gtagcgtcta cataaacccc atccggtttg acgttcaacc cgtctactgt ttcgttcagt
     6601 agaacagaca catgttcaaa tgccattgtc ttctccttat caaatacgaa ttcctatgga
     6661 ttccatacgc tctgcaatcg cgtccagatc ttcctcgctg atctgactgc gttcctccca
     6721 aagagcctta tcccatatct cgatgcgatc ctgaactccc actaagacta catctttttc
     6781 taatccggct gctttgcgga gcgacggcgg gacaagaact ctcccctgct tgtcgaaact
     6841 gccctccgaa gcgcttccga aaaaataacg cttgaaaatt ctggcatcct tactcatgcg
     6901 tggcaaagtc tcaagttcag caacgaattt attccattct tcctgagagt aaacaaagag
     6961 gcagccttcc agtccgttac aaataacgaa actgtcacca agatcatccc gcagttttgc
     7021 cggaatgatg agtcttccct tttcatcaat actatggtta aactctccta ataacatctg
     7081 gaatcctttc tcttaatgtg agctccacat cgcccctact ttactccact agcctccact
     7141 ctttaccact tgactccatt ctaaaccacc actccccctt tttcaaccct tttttgagat
     7201 ttttttgtgt ccttttttcg gctaaaaacg aatcaaaaaa caggattttt cgcaaaaaaa
     7261 gaacatgtat ggcattgatg ttccaaatac cctacatgtt gtgtttttaa tgaatttatt
     7321 cactttaaag ttttaaaaaa aatttgggtg caagggtgtg cgctcttcaa attcggcgct
     7381 cactccccac accacaccat ttactcagca gaaccttctg cattcagtcc gttcaaataa
     7441 tcagatacgg cgtcataatt cacctctgag acagttctct cttttcccga ctcatagata
     7501 tcataagcag aataaccaag ccatccgatc aatgccagag ctaccaccac aagcacccct
     7561 ttacgcatat tattcatcag cttctgctta cgcatgatct gttttctgtt tactttttcc
     7621 tgtttataac ggtcaacctt ttcctgactc atcccgtatt actcctttca gataagatac
     7681 tacgcatgag gacattttat cacaaacact tctacagtac aactattatt ccaccgtcat
     7741 ttgcatacat tgtgctgatt ccgcctgtct ctactataaa actgtctttt acttttatct
     7801 tatcgagaat catacgctct atctctcttg ctctttccgg gcagttgcag tgagcgatcg
     7861 ccaacacttt attctctgta tttttgccat ctgccacaat atgctctgcc atcttcgcaa
     7921 tcgctttctt aactcctctc gcctgaccaa gctgacaaat cgttccctcc ggagttgacc
     7981 ccatcaccgg tttgatatta agagcgcttg ccatcaacga cttaattcct gtaagccttc
     8041 cgttcttccg aagtgtatcc agattttcaa gaacaaaata tgtgtgctgt tcttcgatat
     8101 atgcttccac atgctctatc acttcttcaa aagaataccc tttttcttca cactcttgta
     8161 ttttcaatgc gatcaaactt tcacctaccg atgccgaacg cgaattaaat acatagattt
     8221 gtttatcccc gtcttcgttt tcttccttcc acagctcttt tccaagctga gcgctattat
     8281 acgatccgct taattccgct gacaaggtca ccacataaac cctcttctcc gctccgccgt
     8341 aaagttccat atatgcatcc ggcgaaggac aggaagactt cggacattcc ggactcaccg
     8401 ctactctttt cagaaaatct ttccggtcaa aactttcatc atccaagatc gtatctccgt
     8461 caacgagcat actcaaaacc gccgtatggt aaatcccgct ctccttcatc ttatatgtca
     8521 attctccgca actatccaaa acaattttat aatccataac acttcctcct gaaacgcaaa
     8581 atatatttta cgcttctatt gtatgtagtt tttaatacca catataaata tatcacacat
     8641 ctcataagaa aggaagcttt ttctattcta atcttatttt ccgtgaaatt ccaagcgcaa
     8701 ttcccatttc cgccatcaaa aatacgattg cagttccgcc gtaactgata aaaggcaacg
     8761 taatacccgt tgtcggaaga agtcccgtca cgacagcaat gttcaaaata acctgtaatg
     8821 cgatatgcgc aaatatccca gtcgcgatca gcgaaccgaa aagatcgggc gcatttttcg
     8881 caataaagat cagtctgtat aacaacaatg caaacaggac caaaataaca accgcgccaa
     8941 atacgccaag ctcctcacaa ataacgacaa ggatcatgtc attttgcgct tcgggaatca
     9001 cgcccaattt ctgtgtactg tttcccagtc cttttccgaa cagtcctccg cttccgatcg
     9061 cataaagtcc ctgcatgacc tgaaaacttc ccgtatcggc cgtagcttcc gggttcagcc
     9121 acgagatcac tcgctggaga cggaaattat cactgttcgc cacagttacc gaaagaattg
     9181 cgatcccgac tgccgctacc gcaatcccga tcccgattat tacaagaaac ggcttcgtct
     9241 tcggatggct tacaaaaatc aaaatacatg taatccccgc cacgatgatc gccgtactca
     9301 ggttatctgt caaaaacaaa acgcctgccg atgccactgc gccaaatgca agtatctgtg
     9361 caattccttt cggcgaatat gcccttttcc caagacggca taactcataa gatataaata
     9421 gaatcactgc tatttttgta atctcggcag gctgcagcga aaggttgccg ggaagtccga
     9481 tccaacgtct cgcgccattg acagtcacgc caagcggagt ctgtacaagc gccatcaaaa
     9541 acatcgaaat tacatagatt tcaaatgcaa acgctccata cagatgataa tctaactttg
     9601 acacaagata catgccgata aatccgccca caccgatcaa cgcctgtttt gaaaaatagt
     9661 acatgtcatt tccgaaatca gcctgcgctt catagaaact gacactataa agcataacaa
     9721 gaccaaaaca catcagaaaa ataatcacaa aaagaaggtc atagtcaaaa tattgttctt
     9781 ttcccgctgc acttctttgt cttgcgatca gcgccgataa actatttctg ttttcataac
     9841 gagttctgcc tgacgtacgt tcctttttcc ctttgcggga attcacacgt ccagaaaccg
     9901 gacgctccgt ccgtatcact tcacgcctac ggtcaatcgt tctgcggtat ccctcatacc
     9961 cgcttctgat attcatcgga cgcatcggcg ccgtatcgta cacatcttct ctgacgggac
    10021 gcatcggcat cgtatctccg aggtttcttt tcttttgctt ctttacattt ttcctgccct
    10081 gttcgcgtct cttgatcgga cgcacagtcc tgcttctcat atcatcacac ccatcatcaa
    10141 tcttatttac ctgctgtcat ggaaggatgc accggtttcc catgttcaaa aaagcggtca
    10201 taccatacaa ggaaaatata taccgtccag attttgcggc tgtcatctgt tttttcattc
    10261 gtattctttc cgtctctgtg atcttccagc atcttcataa gcagatcggt attaaagaat
    10321 tttttcgcca tagaagatgc aaacatcgct ctcacacgat tataatattt ttcctctttc
    10381 agccaaaccc gaatcgggat cggaaaacca agtttctttt tctctgccgt cttgtttctg
    10441 atactgcgtt ccgccgctgc ccggagagca actttcgtgc gcggtgcctg cactttatat
    10501 tgaagaggaa ggctttccgc aagttccaga acattccggt caagaaacgg cactctgact
    10561 tccaaagaat tcgccatgcc catcttatcc cctttcataa ggatatcgtg aacaagccaa
    10621 agatgcaagt ccacatactg catttttgtc accgcatcct tatctttcac ccggttatac
    10681 aaaggttttg tcacatcctg aattcccggt ctgcatccct ttttcaaaat tttagcagcc
    10741 tcacgctctg taaatatatt cgtagcattg gcaaaatacc gttcttccag cgtctttcca
    10801 tgacgcataa gaaatccacg gcctttcatc cccctcggca gacagtattc tgcaaatttt
    10861 cccataaagc gccggatcgg catcgggatt ttattaaaag cagtatgttc aagaggttca
    10921 cagtaaatat tatatcctcc gaacaattca tccgatcctt cacccgaaag cacaactttt
    10981 actttctttg ccgcttcttt actcaaaaaa tacagtgcaa ccgccgcagg atccgccaca
    11041 ggctcatcca tataatattg aatatccgaa agactgtccc agtattcatc cggcgtgatc
    11101 accttcgcat cattcttcat atgaatactt tcggcaaaat cttttgcatc ctgaatctca
    11161 ctgtattctc cctcgtcaaa tccgactgta aacgtatgat cgacttgtcc cagataagtc
    11221 aggtaactgg aatccactcc gcttgataaa taagacgcaa cctccacatc gctaatctta
    11281 tgcttctcta ccgactcctt catgactttt tccacttcag ccgcagcttc atcgaatgtt
    11341 ttttcatatt ttcctgtaaa attcggttca aaataccgcg atatttccat tttcccatct
    11401 tcataaataa agtaatgacc gggctgaaga caaaatactc ctttgaaaaa ggtttcgtta
    11461 gtcggtacga actgaaagga aagataattt cccaaagcat cttcattaaa aatcttatca
    11521 aatttcggat gttccataaa tgctttgatc tcagacgcaa acaaaaaggt ctgattcatc
    11581 tgcgcatagt aaaaaggctt gattccaaaa atatcccttg caccgaaaag tctttttttc
    11641 tttgtatccc aaatcacaaa cgcgtacatt ccccggagac gatcgacaag tttctctccc
    11701 cactgctcat atccgtgtat cagagtttcc gaatctgtat tagagacaaa tacatgtcca
    11761 gcctcgatca attcttttcg cagttcctga tagttataga tttccccatt gaaaacaagc
    11821 accatgcttt tatcttcatt ataaaggggc tgatctccga ctgaagacag gtcgataatg
    11881 ctcaaacgcc gaaatccaag agccgcatcc tcatcaacat atttccctgc actgtccggt
    11941 cctctgtgta tgattgtatc catcatattt acaagaacag tttcccgatc ttctacttta
    12001 ccgacaaaac ctgcaaaacc acacattttt cttcttcctt tccataatat ccgaaataat
    12061 agataaggaa tcagtttccg gtaagccatt tacctgatct gatccctcat tgatctctcg
    12121 tatatgatat cttcttcaaa acaaaaatat acttctgcaa tcaccggaag tgccaaaaag
    12181 cacgctccga aatcgcaagc gtccgccaaa atctcgggca agcccgaact tcggctcgcc
    12241 gccgcaatag atcagtcggg gtgacaggat tcgaacctgc gacctcacgg cccccagccg
    12301 tgcgctctaa ccaaactgag ccacaccccg atgacttacc tattatatat aatttaatgg
    12361 cagttgtaaa ggatttttcc atatatcatt cgcaaaaacg taagtcgttc atattataat
    12421 aatgaattta caacaaaagg agataccgcc aatggcaact tgtcaaacaa ataatcagat
    12481 gcgttatatg cgccgtacaa tgcccgacac ctcctgcccg tgtcacgaaa cagttcctat
    12541 gaacggatgt ccgaataccc gggacttttt ccctgcaaat atgccgattg caatggcata
    12601 tgttccatgg caaaaatggc agaacattta tgaaccgtgc cgcgctctta aaaacggaac
    12661 gatctttgag gaattaaata aaccattctc agggaaagga gcctgctgcc gatgaaaaac
    12721 tatccttatt ctaactcaaa ctgttctgtc acaaataata atactcaaga ctgttcttta
    12781 aataacgctt caatgaaaaa tatgcatcgt tctatgaacc gaaaccagct tatggagcat
    12841 atcaaccagg ttagttttgc tgttgacgaa gtaaaacttt atctcgatac gcatccttgc
    12901 gacagcgaag cgcttgcgta ttttcatgaa atgagcgccc accgcaacga cgctttaaaa
    12961 caatatgctt ctgcctacgg accactgact gtcgacacag ccgattattc ctgtgccgaa
    13021 cgatggaact ggatcaatga accgtggcca tggcaggaag gaggatgctg atttatgtgg
    13081 aattatgaaa aacgtcttca atatccggta aaaatcacac aaaccaatcc taaaattgca
    13141 aagatcatca tcagtcaatt cggaggtccc gacggagaac tggccgcatc tatgcgctat
    13201 ctctctcaaa gatatactat gccgtataag gaagtaacag gaacgctaac cgatatagga
    13261 acagaagaac tcgctcacat ggaaatggta tgcgccatcg tttatcagct caccaaggac
    13321 ctttctccgg aggaaatcga acgctccgga tttgctcctt attatgtaga tcacacactt
    13381 gctctctggc cacaggcagc aagcggcgcc ccatggacag caacttattt ccaatcaaaa
    13441 ggagatccca tcaccgatct tcatgaagat cttgcggcag agcagaaagc gcgtacaacg
    13501 tacgataata ttcttcgtct cgtaaaagat cctgaagtat gcgatccgat ccgtttctta
    13561 agacaacgtg aaatcgtcca ctaccaacgt ttcggtgaaa gtttacgcat tgtacaggaa
    13621 aaactcgaca gcaaaaactt ctatgctgtc aatcccgagt ttgataaata atcactgcaa
    13681 cacaaacttc ccctcccgtt tcagagtttt ctctctccgg gaggggaaat aatttttcat
    13741 ttatatattt tcagaaaaag gatttctctt tcacacttac tgcagcgtca cttctatagt
    13801 ctgctcttta tactctccgt ttgtctgagg aatctggatt tttaacgtca cagttgtacc
    13861 tttctcatag tattgcagct gctcctgaag agcctccatg ctgtctactg ttatgtcatc
    13921 aatagctgtg ataatgcacc cttttgtcat tcctgctttt tctgcaccgc cgccttttgc
    13981 aatttctgtt acatatactc ctgccggcat ctcaatctgc tgtgaaaagg catccgaaac
    14041 actgattccg gttatgccga taaggcctct tttatcttcc gcaacttttg tttttgtttc
    14101 tctgttcata aggttttcaa tcaaatcacc gacatcactg atcggaattg catatccgat
    14161 tccttccaca ctgtcaccta caagttttga agagttaatg ccgatcactt ccccatttac
    14221 attgacaaga gcgcctccgc tgtttccttc gttgatcgcc gcatccgtct ggatcaattt
    14281 cacaccgctt tcctgtgtat ctccagtctc ttcatttgtc atagaaacag aacggttcaa
    14341 tgcactgata acgcctgttg tcacagactg tccgtatcca agtgcatttc cgatggcgat
    14401 cgcaggttcg ccgactttca ggctcgtgga atcacccaga gtggcaactg agattgcatc
    14461 cagtgtatca tccgaaatat tctcaatagg cactgcaata actgcaacat catactgggc
    14521 atctgtaccc ttaatgtttg cttctacagt tgattcatcc gtaaaggcaa ctgtaagagt
    14581 atcgcttccc gcaacaacat gattgttcgt cacgatcaat aactctgtgt ctgtttttcc
    14641 gataataatt ccggtacctg cgccttttga ctgctgttca taagttccgc cgaaaaagct
    14701 gcggacttct tctaccgaca tacttgtgat agaaacaata gacggcatca cctgatccac
    14761 aacttcagat acatcagatg tcactacgct ggatgatttt gaaagaggag tccctttact
    14821 taccttatca gaagatgcgg ttgtagattt tgaagagtca agcaacccaa acacccggtt
    14881 tccaaccacc gttgttgtaa ggaaaatccc acttgaaaca acaccgaata aaatagcaaa
    14941 acctacgact gcaaccgtcc gcatcaaatt cttatgcggc tttttttcct ttttcaaatg
    15001 atcgccgccc ggctgccact gtggggtatt tcccttttgt tctccgtttt ggctgccttc
    15061 gctctgagaa ttcattctct gccgatcttc gttatttgaa ttttcatttg gattataata
    15121 attatattga ttgtccataa cactacccgc tttctttctt tttttctatg gtcttatcat
    15181 agcggattaa tgtgatgaat ttgtgtttat tcatttaaca aatattgaat tgaaaacaca
    15241 aaaaaagacc ggacttagaa aatgattgaa aatttc
//
LOCUS       DBMDXF010000023        15144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1162_length_15144_cov_2.126637, whole genome shotgun sequence.
ACCESSION   DBMDXF010000023 DBMDXF010000000
VERSION     DBMDXF010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 15144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15144
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_15144_cov_2.126637"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="ACZPD4_01880"
     CDS             <1..581
                     /locus_tag="ACZPD4_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1377861.1"
                     /translation="VTAAEHSIGSQEAPDSARIELKNLLNDYEIYKNREEDLMSLTEE
                     KLSEIPHIDKLLEIKGVGLKTVSGFVAEVGDITRFDNPKQLQKLAGYAIVECSSGKHK
                     GESHISYRGRKRLRYVLYEAAISLIARNEEFKEVHRYYRTRKENPLKKMQSVIAVACK
                     VLRIFYTILTKGVTYDGAKMMQDIKRPQMLAA"
     gene            1443..3590
                     /locus_tag="ACZPD4_01885"
     CDS             1443..3590
                     /locus_tag="ACZPD4_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR1377862.1"
                     /translation="MHALRAGNVTGDQLTFHNKKLMMYICQSYFYEREEQAGMLGDKY
                     ICVDSTFNSRLKVWDSDVYKSEEPEDTEVFFLPRNNEEGGAFDPNASVGTRTIYREET
                     DSIVTFKDNSYIAEYDIQSGEEIYSAKTDYPVFCLMEYEGGVAAVEKSGDNYYYEFFP
                     WKKATSLEVEGSSHTVNIGDTLQLTPVTNGTMEEVYSWKSSDPKIASINRAGQVFGWS
                     KGEAVITVSTTSGLSAQCTVTVTGEPSVQDPDKTATVTDGYASNNHSLNDYAIWSKTV
                     NSYLIPNADGTFTRVEHCGGKMIVENYSAAGKKTGSKTVAMELSLFGGFYSGSDHNYF
                     VFGQANPNDSDEEEVMRIVKYSKDFQRIGAVSVYGANTNIPFEAGSLRMTETAGKLYI
                     HTCHEMYTDSDGLNHQANMTFVINENDMSVEQSYYDVLNLAQAGYVSHSFNQFIQTDG
                     ENIYRVDHGDYAPRGIALIKSQVGGSITKVDYAIPVGLGKVTGGHYNSTGASVGGFEI
                     SSENCIIAGNAVDFESESANTSDQRNIFISITDKQLTQAKTVWLTNYDKQQGINVQTP
                     QLVKIGEDQFLVMWQEGSKSEGNLTTKIVTIDSEGNKTSNIRSKSMPLSDCQPVVGPD
                     GVVRWYVTDGKAPTIYAVNPFEQSQSYIKGDVNEDGKVEISDLRLILRSVCKKVELTE
                     QQKLAADVTEDGKVEIGDLRKVLRYICHKIPEL"
     gene            complement(3666..4997)
                     /gene="mgtE"
                     /locus_tag="ACZPD4_01890"
     CDS             complement(3666..4997)
                     /gene="mgtE"
                     /locus_tag="ACZPD4_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1377863.1"
                     /translation="MNKDIFVQLLGQRQFKAVRSILDVMNEVDIASLLSELDDKELAL
                     AFRLIPKDKAAEVFANMETSMQTYLVDMFSEKELKELLDDLYMDDTVDLLEELPANLV
                     NRILDTVSPSDRTLINQLLNYPEDSAGSIMTTEYVDIRQSMTVAQSMAHIKETGIHKE
                     TIYTCYVTDKRKLIGIVSAKDLMTTDDGILISELMETEIISVRTYTDKEEVAQLFRKY
                     DLLALPVLDKDGLMVGIVTFDDAMDVMVEEATEDITKMAAINPSEKTYFNTSTFAHAK
                     NRIPWLLILMLTSIITGTIITKYEDAFAAIPLLVSFIPMLMDTGGNCGSQSSTLVIRG
                     LALSEIHFKDIFKVIFKEFRISIIVGAVLALTNGVRILIQYHNPNLALVIALSLIATV
                     IISKLIGCILPLVASKVHLDPAIMASPLITTLVDTFSILIYFNIASLMFRL"
     gene            5290..6750
                     /locus_tag="ACZPD4_01895"
     CDS             5290..6750
                     /locus_tag="ACZPD4_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HIR1377864.1"
                     /translation="MEYKITGCKPEKLFHFFEDISAIPRGSGNEKGLSDYLVKFAKDR
                     NLWVYQDESNNVIIKKEGSEGAKDKAPVMLQGHIDMVCDKLAGVEHDFEKDGLDLIVK
                     DGVLYANGTTLGADNGVAVALMMTVLDDKELEHPPVECVFTTSEEIGLNGAQALDKSQ
                     ITARTMINMDSEEEGVATVSCAGGLRVQLTRKIERVQAEGTLVQIKAEGLLGGHSGTD
                     IDKERQNANLLMARMADHLLKNTKGLLVNFAGGTKDNAITRECEMSLIYADSAEAQKA
                     EKLACDLAEVFADEITSFEPDFSCEIAVTEGQSAMAVQEEDAKAFVSLMRLAPNGIYR
                     RNIKMNGFVVVSSNMGVVRTEEDYIMVAVSPRSSVASLQEDTKSRFALLAETFGFEIE
                     YSGEYPGWSYAEESKIRDLFVESYRDLFGSELRLEAIHAGLECGLFSEAIPGLDAIAV
                     GPTLNNVHTPDENAPLDSLERFYELLKDVLSRLARA"
     gene            6813..7913
                     /locus_tag="ACZPD4_01900"
     CDS             6813..7913
                     /locus_tag="ACZPD4_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR1377865.1"
                     /translation="MPLFLCDREFVSDQRWRVDMNEFYEELKKVLDLSDGDVWTETVL
                     CGKDAGKKRILSGIFAQDEEQNERPDEKKKENREKSGCEGKSRIFRERIGRIPRLVIC
                     GGGHVSAAATKLGKMLGFEVTVLEDRPKFADHVRKAGADNVICAPFKEGLAKIGGDSD
                     TWFVIVTRGHRYDAECLEIIVEKQNAYIGMMGSKRRVAIVKDQLEKKGIDREDLERVH
                     TPIGLKIKAETPEEIAVSIMAEIIEVKNSREKAGGYSKELSEKLFEKKNDTKESFKEP
                     SLKKVLATIVSRKGSAPRSVGTKMLIMEDGSITGTIGGGCVESEVMQKALLMMRKGEP
                     VFQLCKVDMTADDAEEEGMVCGGVVEVMLEMI"
     gene            7938..9224
                     /locus_tag="ACZPD4_01905"
     CDS             7938..9224
                     /locus_tag="ACZPD4_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1377866.1"
                     /translation="MKRKSVFILKGNIVYSKNQKEFSICENGYVVCKEGIVEGVYKTL
                     PFKFGGNPIRDYKDALIIPGFTDLHVHAPQYSYRGLGMDMELLEWLETNTFPEESKFC
                     DLDYAEKSYRIFVKNMQKSATTRACVFATLHRPATVLLMDMLEQSGLSTFVGKVNMDR
                     NAPDYLVEETKKSTEETLKWIEETIQKNYSRTYPILTPRFIPTCTDEVMKELKKLQKR
                     YELPLQSHLSENFGEIAWVKELCPWSEFYGDVYDTFGLFGKDTRTIMAHCVHSDEREI
                     SRMKENGVFIAHCPESNMNVSSGIAPIRKFLEEGLHVGLGSDVAGGSTENMFRAMAHA
                     VQASKLRWRICDDSLEALTAEEVFFMATKGGGEFFGKVGSFEPGYEFDAVVLDDSRLA
                     HPQELDIRSRLERMIYLADEREVVGKYVKGKEIDLS"
     gene            complement(9340..9723)
                     /locus_tag="ACZPD4_01910"
     CDS             complement(9340..9723)
                     /locus_tag="ACZPD4_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1377867.1"
                     /translation="MLKYVETNKAPAAIGPYSQGIVVNGTAFFSGQIPLSPETGEVIG
                     TTIREQTEQVMKNIQGLLESQNASFTDVVKTTCFLADMSDFAAFNEVYAKYFTGKPAR
                     SCIAVKDLPKGVLCEVETIAVIEEN"
     gene            10026..11471
                     /locus_tag="ACZPD4_01915"
     CDS             10026..11471
                     /locus_tag="ACZPD4_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1377868.1"
                     /translation="MGIRETKKDNSELFKWDGKPSAGQVFPLAAQHVLAAVVGCVTPA
                     ILVANAANNAGGNVDMGLLIQMSLVFAALSTLLQLYGNKIRIGSGLPVIIGVSFAYVP
                     TMTAIAAQAKSIDTILGAMLIGGVIAIIVGLTIKQIRKVFPPLVIGTVIFAIGLSLYK
                     TAINYMAGNPANTYELVVEQQGKTPALVFGSWQNWVVAFVTLAIVVALNHYGKGLFKL
                     ASILIGLLCGYVLALCFGMVDFSVLSSAGWFQIPKPMEFGMKFEISAIIPLAILFLVN
                     SIQAMGDFSATTSGGMDRLPTDRELNGGIIGYGIGNIISAFFGCPPTATFSQNVGIVG
                     TTKVISRRVFATSAGILLVAGLIPKFSALLRTIPQCVLGGAVVSVFASIAMTGIRLLV
                     TEKLTARNATVAGLSIAIGMGVSLSGGCLDQMTQSIHSALNLSMNLENFQAIINNTFA
                     SSPVVLATIFAVILNLILPKEKTTEKETKLR"
     gene            11529..12896
                     /gene="hydA"
                     /locus_tag="ACZPD4_01920"
     CDS             11529..12896
                     /gene="hydA"
                     /locus_tag="ACZPD4_01920"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR1377869.1"
                     /translation="MMKRLFKGGTVVSGEGLKKIDLLVKGEKILAAGKNLEFQDAETI
                     DVRGKLLFPGFIDAHTHMAAKFNDITAADKFETGTKAALAGGTTCIIDFAEQKKGKSL
                     KEAIDDKRQEAEGEASCDYALHLTLNEWNDDVAEELGEIVKKETCSFKLYTAYDMMLD
                     DGQIYEIFSRVKELGGIVGVHCENHGIVQSRLKELEKTKGRTDIADYSWIHPKEAEAE
                     AVGRILKIAKCADVPVIIVNLSTAAGYREILKAREIGQTVYVETCPEYLLLNEEKYAL
                     AAEEARKYVTVPPLRKQKNSDILWDALKEGRIQTIASDHFGFTKEQKNAGEEDFMLTP
                     SGMPGAEERPALMWQFGVNEGKITAEQMCAYLSENPAKLFKLYPWKGALIPGSDADIV
                     IWNPNTEWTMTAADHQSACDYCLLEGAEIEGRAEQVYLRGKLVAENGKIIEEKTGMYI
                     RHGVE"
     gene            12961..13644
                     /gene="yqeC"
                     /locus_tag="ACZPD4_01925"
     CDS             12961..13644
                     /gene="yqeC"
                     /locus_tag="ACZPD4_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR1377870.1"
                     /translation="MNTSVISVVGAGGKTTLLHRLEDECVRKKQHVIITTTTKIMHEN
                     KPYFLEDTSIGKIKDTLGKFGRVWIGSRDLISGKTAPPAKEILDEIFWWELPILVEAD
                     GAKRLPLKVPAEHEPVIPSQTGHVVSVYGLDAIGRTLESTCFRWERAAQILEKGGEEF
                     VTAKDIAILASSHKAGRKGCPPYAQYTVVLNKADSASKREAALEVCRELNVRGVENIV
                     VTSLHSKKL"
     gene            13668..15095
                     /gene="yqeB"
                     /locus_tag="ACZPD4_01930"
     CDS             13668..15095
                     /gene="yqeB"
                     /locus_tag="ACZPD4_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR1377871.1"
                     /translation="MKILIKGAGDLATGIASRLYGAGHQILMTEIAQPLTVRRTVALS
                     RAVYDRRAIVEEMEAVSAKTQEDAEAIMEEGKIPVIVDPKADCRKWYKPDVIVDAIIA
                     KKNTGTKITDAPFVIGIGPGFTAGKDCHCVIETKRGHTLGSVIWEGSAIADTGVPGNI
                     GGYTTERLIRASEDGIIEPKVRIGDIVEKGQIVAVTGGKPVFAQMGGIVRGMLQPGAY
                     VKKNLKIGDIDARAERSHCETISDKARAIGGGALEAAERFEKIKGHYAVVILAAGKGT
                     RFSGERGESKLHAKIDQKPMYMHMMDKMQAFSSVELFIVTGDEKIIEEAEKRGIFVVR
                     NKEPEKGIAHSVVLGLKAVSHSFKSGTDRKSGAPDGILFSVCDQPKLKISTIQRILND
                     AALHKKNVICAGYRGQKGNPVLWPAHCFAELLELTGDEGGRQMLTKYEEKVRIIETSR
                     EELKDIDSKRDLENLGKETEENKKA"
BASE COUNT         4790 a         2587 c         3975 g         3792 t
ORIGIN      
        1 tggtaacggc tgcagagcac agtattggaa gccaggaagc acctgatagt gcaagaattg
       61 aattgaagaa tctactgaac gattatgaaa tctacaaaaa tagagaggaa gacctgatga
      121 gcctgacaga ggaaaagttg tcagagattc ctcatataga taagttgttg gaaatcaaag
      181 gagttggatt gaaaacagtg agtggatttg ttgccgaagt aggagacatc acacgttttg
      241 ataatccaaa gcaactgcaa aaactagctg gatatgccat tgtagagtgt agttcgggaa
      301 aacataaggg cgagagtcac atcagctaca gaggaagaaa acgcctcaga tatgtactgt
      361 atgaagcagc catatccttg attgcaagga atgaagaatt taaggaagta caccggtatt
      421 atcgaactag aaaagaaaat ccgctaaaga agatgcagtc agtcatagca gttgcgtgta
      481 aagtcttaag aatcttttat acgattttga ctaaaggtgt gacttatgac ggtgcaaaga
      541 tgatgcaaga tatcaagaga ccacagatgc ttgcagcata gagtgcagga agtaaccaac
      601 ccgaaaaggt acaaaagcag aatgtaatgg ggaacgtcca ctgaaaagtg ctgttacagg
      661 aagcaagtca gtaataatag gaacaaagaa tgagcaggta gttggcagga atattttcca
      721 tagggcaaga ccctgacaag gagctaagct gacacccgga ttatggatag gcagaacgaa
      781 ggaagttagg actcacgaag tgatgatcct ggtagacacg ggaggtgcgt tgccatagat
      841 ggaagggaat acacaaggcc atttaaaacg aataaaaaag acgttttatt tcgtataccc
      901 taaactagct attttcgtac cagatacaga gaaacatcca tgattcttgt tcattcatct
      961 gagatagaaa ctttaaaaaa cctttatttt aaaggaaaaa agacttgatt tattagggag
     1021 gaatttattt tctgaatcgg aacaaattat cgttgtacaa gccggacagc aatgagttga
     1081 cagaagtaca tcagtttgac aatgtgcaag atgtttatgt ggcaaatgac agactgtata
     1141 tattggaccg cagttctaat attacgatct ataatttgct tacgcagcag gaagagaaga
     1201 aacttcagta tggagatcac gcaagttcga tcggcgcaga ttcaaaggga aggatttatc
     1261 ttgccgaatc gaatggcaat ggtgacgatt atgatctgta tcttttatcg ccggaaggaa
     1321 cgcttttatc gcaggcttta tccgaggagg cagtgtacgg tttttgcggc ttcgatgaaa
     1381 gtaacgggaa ctattatgta gatacttata ataactggcg gtactggggg tatgaccatg
     1441 atatgcatgc ccttcgcgcc ggaaatgtaa ccggagatca gctcaccttt cataataaaa
     1501 aattgatgat gtatatttgt cagtcatatt tctatgaacg tgaagaacag gccggtatgc
     1561 tcggggataa atatatatgc gtagacagta catttaattc aagactaaag gtttgggatt
     1621 ctgatgtgta taaatcagaa gaaccggaag atacggaagt tttcttctta ccgcgcaata
     1681 atgaagaagg aggagcgttt gatccgaatg cgagcgtcgg aacacgaacg atctaccgcg
     1741 aggagacgga ttctattgtt acatttaaag acaattcata catagcggaa tatgacatcc
     1801 aaagcggaga agagatttat tctgcaaaaa cagattatcc ggtattctgt ctgatggaat
     1861 acgaaggcgg ggttgcagcg gttgaaaaat ccggcgataa ttattattat gaatttttcc
     1921 catggaagaa agcgaccagc ctggaagtgg aaggaagcag ccatacggta aatataggcg
     1981 atacattgca gcttacacct gtgacaaacg gaacaatgga agaagtctac agttggaaga
     2041 gcagcgatcc gaaaatcgcg agcatcaacc gggcgggaca ggtattcggc tggagcaaag
     2101 gagaggcagt gatcacagtc agtacaacaa gcggtctgtc ggcgcagtgt acggtgacgg
     2161 tgacgggaga accttctgtt caagacccgg ataaaacagc gacggttaca gatggttatg
     2221 cttctaataa tcattctttg aatgattatg caatatggag taagacagtt aattcttatt
     2281 tgattccgaa tgcagacgga acatttacga gagtggagca ttgcggcgga aaaatgatcg
     2341 ttgaaaacta ttctgcggca ggcaagaaga ccggaagtaa aacggttgca atggagctga
     2401 gtctgtttgg cggattttac agtggaagcg atcataatta tttcgtgttc ggacaggcta
     2461 atccaaatga cagcgatgaa gaagaagtaa tgcgaatcgt aaaatattcc aaagattttc
     2521 agagaatagg ggcggtttcc gtatacggag cgaatacgaa tattccgttt gaggcaggtt
     2581 cgctgcggat gacagagaca gccggaaagc tttatattca tacatgtcat gagatgtata
     2641 ctgacagcga cggattgaat catcaggcga atatgacgtt tgtgatcaat gaaaatgaca
     2701 tgagtgtaga acagtcttac tatgatgtgc tgaatcttgc acaggcagga tatgtaagtc
     2761 attcttttaa tcagtttatt cagacggacg gagagaatat ttacagagta gatcacggtg
     2821 attatgcacc gagaggaatc gctcttatta agtcacaggt tggcggatcg atcactaaag
     2881 tcgattatgc gattccggtt ggtttaggca aggtgaccgg agggcattac aattctacgg
     2941 gagcctctgt cggcgggttt gaaatatcgt ctgaaaactg tattatcgca ggaaatgcag
     3001 tagatttcga gagcgaaagt gccaatactt cagaccagag aaatattttt atcagcatta
     3061 ccgataaaca attaactcaa gcgaaaacgg tatggctgac aaattacgat aagcagcagg
     3121 gtataaacgt acagacacct cagcttgtga agatcggaga ggatcaattc cttgtaatgt
     3181 ggcaggaagg cagtaaatcc gaggggaatc tgacgacgaa gatcgtaacg atcgattccg
     3241 aaggaaataa aacttctaat ataaggtcaa aatcgatgcc gctttccgat tgtcagcccg
     3301 tcgtaggacc ggacggggtt gtaagatggt atgtgacaga cggcaaggct ccgacgattt
     3361 atgcggtcaa tccgtttgag caaagccagt catatataaa gggcgacgta aatgaagacg
     3421 ggaaagttga gatcagtgat ctgcgcctga ttctgcgcag cgtatgtaaa aaagtagagt
     3481 tgacagaaca gcagaaactt gctgcggatg tgacggaaga cgggaaagtc gagatcggtg
     3541 atctgaggaa agtgttaaga tatatttgcc ataagattcc tgagttataa caagaaaaag
     3601 gcgttgattc gttttttacg gatcagcgcc tttttaagca gaaaaagttg tgaaaacaga
     3661 actgcttaca accggaacat taatgatgca atattaaaat aaatcaatat agaaaatgta
     3721 tcgacaagcg ttgtgatcag cggagaagcc ataatagccg gatcaaggtg caccttgctt
     3781 gcaacaagag gaagaataca tccgatcagt ttcgagatga taacagttgc gatcagcgaa
     3841 agcgcaatga ctaaagcaag attcggatta tgatactgga tcaggatacg aacgccgttt
     3901 gtaagagcga gtacagctcc tacaattatg gagatacgga attctttaaa aataacttta
     3961 aaaatatctt taaaatgtat ttccgaaaga gcaagtcccc gaatgaccag tgttgagctt
     4021 tgcgaaccac agtttccgcc tgtatccatc aacataggga taaaggatac aagaagcggg
     4081 atcgcagcaa atgcgtcttc atattttgtg atgatcgtac ctgtgataat ggaagtaagc
     4141 ataaggatga gaagccatgg gatcctgttt tttgcgtggg cgaaagtgga tgtattaaaa
     4201 taagtttttt cgcttggatt aatggcggcc atcttagtga tatcttctgt tgcttcttca
     4261 accataacgt ccatagcgtc gtcaaatgtg acgattccga ccataagtcc gtctttatca
     4321 agtaccggaa gcgcaagcag gtcgtatttt ctgaataatt gagccacttc ttctttatcg
     4381 gtataagttc ttacggaaat gatctctgtt tccatcagtt cactgatcag aatgccatcg
     4441 tcggtcgtca tgagatcttt tgcgctgacg atgccgatca gcttcctttt atctgtgacg
     4501 tagcaggtat agatcgtttc tttgtgaata ccggtttcct tgatgtgagc catagactgt
     4561 gcgacagtca tagactgacg gatatcgaca tattcggttg tcatgatgct tcctgcgctg
     4621 tcttccggat aattaagaag ttggttgatc agcgttctgt cagacggcga aaccgtatca
     4681 agaatacgat ttacgaggtt tgccggaagc tcttcgagaa gatcgacagt atcatccatg
     4741 taaaggtcat caaggagttc ctttagttct ttttcggaaa acatgtctac aaggtaggtc
     4801 tgcatggaag tttccatatt ggcaaatact tccgctgcct tatctttcgg aatcagccga
     4861 aatgcaagag caagctcttt atcgtcaagt tctgacagta atgaggctat gtccacttcg
     4921 ttcattacat ctaaaatgct tctcacagct ttgaactgac gctggccgag aagctgtaca
     4981 aaaatgtctt tgttcatgat aaatacctct ctttatgtaa tttttttcga tcagactgtg
     5041 tatatgacaa ctgacaccgg agtccataac ctcaccacct ttcaatatgg aacgcacagg
     5101 gcgcttccgc aaaataaaaa ccgtcatttt taaagtgacg gaatcataac aatattttta
     5161 ctgctttatt ttagtttaca ggggaaaaag tgtcaagaaa aacatatgta aaaatggatg
     5221 aaaaattaag aaaaatctgt tataatcaat aaagcatatg gcaataacta atagaaaaga
     5281 ggatttatta tggagtacaa gatcacaggc tgcaagccgg agaaattatt tcattttttt
     5341 gaagatatca gcgcaatccc gagaggatcg ggaaatgaga agggtcttag cgactatctt
     5401 gtgaaattcg caaaagacag aaacctctgg gtttaccagg atgaaagtaa taatgtgatc
     5461 ataaaaaaag aaggaagcga gggggcaaaa gacaaagctc ctgttatgct tcagggacat
     5521 atcgatatgg tatgcgataa acttgccgga gtcgagcatg attttgaaaa agacgggctt
     5581 gatctcattg taaaagacgg cgttctttac gcaaacggaa cgactctcgg agcagacaac
     5641 ggagtagcgg ttgcccttat gatgacagtt cttgacgata aagaattaga gcaccctcct
     5701 gtagagtgtg tgtttacgac gtcggaggaa atcggactaa acggcgctca ggcgcttgat
     5761 aagtctcaga tcacagcgcg tacgatgatc aacatggatt cagaagaaga aggagtcgca
     5821 acagttagct gtgccggcgg acttcgtgtt cagcttacaa gaaagataga aagagtgcag
     5881 gcagaaggaa cattagttca aataaaagca gaaggccttc tcggcggaca ttcgggaacg
     5941 gatattgata aagagcgtca gaatgcgaat cttctgatgg ctcgtatggc agatcatctt
     6001 ctgaaaaata caaaaggact tcttgtgaac tttgcaggcg gaacaaaaga taatgcaatt
     6061 acaagagagt gtgagatgtc tcttatctat gcagattcgg cagaagcaca aaaagcagag
     6121 aaacttgcat gtgatctcgc agaagtattt gcagatgaaa tcacatcttt tgagccggat
     6181 ttctcctgcg agatcgcagt gacggaaggc cagagcgcaa tggcggttca ggaagaagat
     6241 gcaaaggcat tcgtaagtct gatgcgtctt gctccaaacg gaatttacag acgaaacatc
     6301 aagatgaacg ggtttgtcgt tgtatcgtct aatatgggag tagtcagaac agaagaagat
     6361 tatattatgg ttgcggtttc tcccagatcg tctgttgcaa gccttcagga agatacaaaa
     6421 tcgcgtttcg cacttcttgc agagacgttt ggatttgaga ttgagtacag cggagaatat
     6481 ccgggctgga gttatgcgga agaatctaag atccgcgatc tgtttgtaga aagctaccgc
     6541 gatctgttcg gaagtgaact tcgtttggag gcgattcatg caggacttga atgcggactt
     6601 ttttcagaag caattccggg actggatgcg atcgcagtag gtccgacact gaataatgta
     6661 cacactccgg atgagaatgc tccgcttgat tcgcttgagc gtttctatga gcttcttaaa
     6721 gatgttcttt caagattggc cagagcataa gaagaacagg gagatttgat taaaaataag
     6781 tgttctgtaa aagaatgggc gggatcttga agatgccgct ttttctttgc gaccgggagt
     6841 ttgtttcaga tcagcgctgg agagtagata tgaatgagtt ttatgaagaa ttaaaaaagg
     6901 tactggatct ttctgacggg gatgtttgga cggagactgt tttgtgcggc aaagatgccg
     6961 gaaagaagcg gattctttcc gggatttttg ctcaagacga agaacaaaat gaaagaccgg
     7021 acgaaaagaa aaaggaaaac agagaaaaaa gcggatgtga aggcaagagc agaatcttcc
     7081 gtgaacgaat cggaagaatt ccccgtcttg taatttgcgg cggaggacac gtttcggctg
     7141 cggctacgaa actcgggaag atgcttgggt ttgaggtgac tgttcttgag gatcgtccga
     7201 aattcgcaga tcatgtaaga aaagccggag cagataatgt catttgcgct ccttttaagg
     7261 aaggacttgc aaagatcggc ggtgactcgg atacatggtt tgtcatagtg acgcgcggtc
     7321 acagatacga tgcggaatgt ctggaaataa tcgtggaaaa gcaaaatgca tatatcggga
     7381 tgatgggcag taaacggcgg gttgcaatcg taaaagatca gcttgagaaa aaaggtatcg
     7441 acagagaaga tttagagcgg gtacacacgc cgataggatt gaagatcaag gcagagactc
     7501 cggaggagat cgccgtttcg atcatggcgg aaataatcga agtaaaaaac agcagagaaa
     7561 aagcgggcgg atattcaaaa gagttgtcgg agaagctttt tgaaaagaaa aatgatacaa
     7621 aagaaagttt taaggaacca tcattgaaaa aagtgcttgc gactatcgtt tcccgaaaag
     7681 gatctgcgcc tagaagcgta ggaacgaaga tgctgataat ggaagacgga agcataacgg
     7741 gtacgatagg cggaggatgt gtagaaagtg aagtcatgca aaaagccctg cttatgatgc
     7801 gaaaaggcga acctgtattt caactttgta aagtcgatat gacggcagac gacgcagaag
     7861 aggaaggaat ggtatgcgga ggcgtcgtag aagtgatgct tgagatgata taaccgaaaa
     7921 ggaaaggagg cagtgaaatg aaaagaaaat cggtttttat tttaaaagga aatattgtat
     7981 acagcaaaaa tcagaaagaa ttttcgattt gtgaaaatgg atatgtagtc tgcaaagaag
     8041 gaatcgtgga gggcgtctat aagacgcttc cgtttaaatt cggaggaaat ccgatacggg
     8101 attacaaaga cgcgctgatt attccgggat ttacagatct tcatgttcat gcgcctcagt
     8161 attcgtaccg cggtctggga atggatatgg aattgttaga atggctcgaa acgaatacgt
     8221 ttccggagga atcgaagttt tgtgatttag actatgcaga aaagtcatac cggattttcg
     8281 tgaaaaatat gcaaaagagt gcaactacaa gggcgtgtgt atttgcgact cttcacaggc
     8341 cggcgactgt tttgcttatg gatatgcttg aacaaagcgg gctgagtact tttgtcggga
     8401 aagtgaatat ggatcggaat gcgccggatt atttagttga agaaacgaaa aagtcgacag
     8461 aagaaacttt aaagtggatt gaggagacaa ttcagaaaaa ctacagtcgt acatatccga
     8521 tccttacgcc gagatttatt ccgacgtgta ccgatgaggt tatgaaagaa ctaaaaaaac
     8581 ttcagaaacg ttatgagctt ccgcttcagt cacatttgtc ggaaaacttc ggggagatcg
     8641 catgggtaaa agaactttgt ccgtggtctg agttttacgg agacgtttat gatacattcg
     8701 gattgttcgg taaagataca cgtacgatta tggcgcattg tgtacacagc gatgaaagag
     8761 agatcagcag gatgaaagaa aacggcgtat ttatcgcaca ctgtcccgag tctaatatga
     8821 atgtgtcttc cgggatcgcg ccgataagaa agtttcttga agaaggactt catgtcggat
     8881 tgggaagcga tgtggcagga ggaagtacgg aaaatatgtt tcgggcaatg gctcatgccg
     8941 ttcaggcgtc aaaactgcgt tggcgtattt gcgacgatag tttagaagcg ctgacggcag
     9001 aagaagtttt ctttatggcg acaaaaggag gaggagaatt tttcgggaaa gttggaagct
     9061 ttgaaccggg atacgaattt gatgcagtcg ttctggatga cagcaggctt gcgcatcccc
     9121 aggaacttga tataagaagc cgactggaac ggatgatcta tctggcggat gaacgggaag
     9181 tagtcggaaa atatgtaaaa ggaaaagaaa tcgatctttc ttaaggaaaa gagaaaaata
     9241 aaagacaaat aaaagataaa taaatgaaga aacagcccgc ggagaatgaa accttcgcgg
     9301 gctgtttctt gaaaagaatt cggcttatcc tttcgatgtt taattttctt caatgaccgc
     9361 gattgtttct acctcgcaca aaactccttt cgggagatct tttacggcga tgcaggaacg
     9421 ggcgggtttt cctgtaaaat attttgcata tacttcgtta aatgcggcaa aatcagacat
     9481 atcggcaaga aagcatgtcg tttttacaac atctgtaaaa gaagcgtttt ggctttcgag
     9541 aagtccttgt atatttttca taacttgctc tgtctgttcc cggatcgttg ttccgatgac
     9601 ttcaccggtt tccggagaaa gaggaatctg accggagaaa aacgcagttc catttactac
     9661 aataccttgt gaatatggtc cgatcgctgc cggtgctttg tttgtttcaa catattttaa
     9721 cattatatct acctcctgaa aaaatatttt gcattctctt atcatacatc aaaaaattcg
     9781 ccttgtatat atttttaggc gaatccgaaa gtgtaccgtg cttgtttttg aggtgtgctc
     9841 agatgaaaag aagaggggga aagcataatg ggcgattatt gcatttcatt atatttaatg
     9901 atattctaat agaagataaa aggcggtatg aacaggaaaa acgggagagg aaaaagagaa
     9961 aaaatcttcg gagattttcc tgtatcaagg aaggataaag atcagtcaga aagaggaaaa
    10021 gtaagatggg tatacgagag acaaaaaaag ataattcaga gttgtttaag tgggacggaa
    10081 agccgtcggc gggacaggta tttccccttg cggcgcagca tgtgcttgcg gcggtagtcg
    10141 gatgtgtgac gccggcaatt ttggttgcaa atgcggcgaa taatgcggga ggcaatgtag
    10201 acatgggact tttaatacag atgtctcttg tctttgcggc gctgtctacg ctgcttcagt
    10261 tatatggaaa taaaatcagg atcggatcgg ggcttccggt gatcatcggc gtgagttttg
    10321 cttacgttcc gacgatgacg gcaatcgccg cacaggcaaa aagcattgat acgattctcg
    10381 gagcgatgct cataggcggc gtgatagcga ttattgtagg attgacgatc aaacagatca
    10441 gaaaagtatt tccgccgctt gtgatcggca cggttatttt tgcgatcggg ctgtctttgt
    10501 ataaaacagc gatcaattat atggcaggaa atccggccaa tacatatgag ctcgtagtag
    10561 aacaacaggg aaagacgccg gcgcttgtgt ttggttcctg gcagaattgg gtcgttgctt
    10621 ttgtgacgct tgcaatcgtc gttgcgttaa atcattatgg gaaaggtctt tttaaactgg
    10681 cgtcaatatt gatcgggctt ttgtgtggat atgtgctcgc gctttgtttt ggaatggtag
    10741 atttttcagt tctttcaagc gcgggatggt ttcagatccc gaaaccgatg gagtttggta
    10801 tgaaatttga aatatcggcg atcatacctt tggcaatttt atttttagtc aattccattc
    10861 aggcgatggg agatttttcc gcaactacaa gcggcggaat ggatcgtctc ccgacagaca
    10921 gggaattaaa cggcggtatc atcggatatg gaataggaaa tattatcagc gcttttttcg
    10981 gctgtcctcc gacggcaaca ttcagtcaga atgtcggcat tgtaggtacg acgaaagtga
    11041 tttcaagaag ggtgtttgcg acatcggcgg gaatccttct tgtagccggt ctgattccga
    11101 agttttcggc tttattaagg acgattccgc aatgcgtgct tggaggcgcg gtcgtttcgg
    11161 tgtttgcttc tatcgcaatg acgggaatcc gactgcttgt aacggaaaaa ctgactgccc
    11221 gtaatgcaac ggtagccgga ctttctattg cgatcggaat gggtgtttca ttaagcggcg
    11281 gatgtctgga tcaaatgacg cagagtatcc acagcgcttt gaacctgagt atgaatcttg
    11341 aaaactttca ggcgatcata aacaatacat ttgcatcctc tccggttgtt ttggcgacaa
    11401 ttttcgccgt aatactgaac ttgatccttc cgaaggagaa aacgacggaa aaggaaacga
    11461 aattacgtta agtaaagaac gaattatttt tataaaaggt ataaaatcaa gtagaaagaa
    11521 ggggattaat gatgaaacgg ttattcaaag gaggaacggt agtcagcggg gaaggtctga
    11581 aaaagatcga tcttttggta aaaggggaaa agattcttgc ggcagggaaa aatcttgaat
    11641 ttcaggatgc ggaaacgatc gatgtgagag gaaagcttct ttttccgggt tttatagatg
    11701 ctcatacgca tatggcggca aagtttaatg atattacggc ggcagacaag tttgaaaccg
    11761 gaacaaaggc agcgctggca ggcggaacga cgtgcatcat agattttgca gagcagaaga
    11821 aaggaaaaag tctgaaagaa gcgattgacg ataaacgtca ggaagcagaa ggcgaggcat
    11881 catgtgatta tgcgctgcat cttacactga atgagtggaa cgatgacgtt gcagaggaac
    11941 tgggggagat tgtaaaaaag gagacttgtt ctttcaaatt atatacggcg tatgacatga
    12001 tgctggatga cggacagatt tacgagattt tttcaagggt gaaagaacta ggcggcattg
    12061 tcggagttca ttgcgaaaat cacgggattg tacagtctcg tttaaaagaa cttgagaaaa
    12121 caaaaggaag aacggatatt gcggattatt catggataca cccaaaagaa gcggaagcag
    12181 aagcggtggg gcgcatcctg aagattgcca aatgtgcgga tgttccggtg atcattgtga
    12241 atttaagtac ggctgccgga taccgggaga ttttaaaagc gagagaaatc ggtcagaccg
    12301 tctatgtcga gacgtgtccg gaatatcttc ttctgaatga ggaaaaatat gctcttgcgg
    12361 cagaagaagc gcgaaaatat gtaactgttc cgcctcttag gaaacagaaa aacagcgata
    12421 tattgtggga tgcattgaaa gaaggaagga ttcagacgat agcgtccgat cattttggat
    12481 ttacaaaaga acagaaaaat gcaggagaag aggattttat gctgacgcct tccggtatgc
    12541 cgggagcaga agaacgtccg gcgcttatgt ggcagttcgg agtgaatgaa ggaaagataa
    12601 cggcagagca gatgtgtgca tatctttcgg agaatcccgc aaaattattt aaattatatc
    12661 cgtggaaagg ggcacttatt ccgggcagtg acgcggacat cgtcatatgg aatcctaaca
    12721 cggaatggac gatgactgcg gcggatcatc agtctgcatg cgactattgc ctgttggaag
    12781 gagcagagat cgaaggaagg gcagaacagg tttatttgag aggaaaactt gtggcagaga
    12841 acggaaaaat catagaagaa aagacaggaa tgtatatccg tcatggcgta gaataatatg
    12901 gtgtttgtct ataaaaataa tgaactgtgt gaacgtatgt ctttgtcaga ggcgctttct
    12961 gtgaacacat cagtcatctc ggtcgtggga gcgggaggaa aaacaactct tttgcatcgt
    13021 ttggaagacg agtgtgtaag aaaaaaacaa catgtgatca ttacgactac aacgaagatc
    13081 atgcatgaaa ataaaccata ttttttagaa gatacatcta tcggtaaaat aaaggatacg
    13141 ttgggaaagt tcgggagagt gtggatcgga agcagggatt tgatttccgg gaaaacggcg
    13201 ccgcctgcaa aagaaatttt agacgagatc ttttggtggg agctgccgat actggtcgaa
    13261 gcagacggag caaaacgact tccgttaaaa gttcccgcag aacatgaacc ggtcattccg
    13321 agtcaaacag gacatgttgt ttccgtatat gggttagatg caataggcag gacgttagaa
    13381 tcaacctgtt ttcggtggga aagagcagcg cagatcttag aaaaaggcgg agaagagttc
    13441 gttacggcaa aagatatcgc gatacttgcg tcatcgcata aggcgggcag aaaaggctgt
    13501 ccgccttatg cgcagtatac ggttgtttta aataaagcag acagtgcatc taaaagagaa
    13561 gcagcgcttg aagtctgcag agaattgaat gttcggggag tggaaaatat tgtagtgaca
    13621 tctttgcatt caaagaaact ttagaataaa aataagagga aaaaagtgtg aaaatattaa
    13681 ttaaaggcgc aggggacctg gcgacgggaa tcgcatcgag actgtacgga gcaggtcatc
    13741 agattttgat gacagagatc gcacagcccc ttacggtaag gagaacggtt gctttgtcaa
    13801 gggcggtata tgacaggcgg gcgattgtag aagagatgga agcggtatct gcaaagacac
    13861 aggaagatgc ggaagcgata atggaggaag gcaaaatccc tgttatagtt gatccaaagg
    13921 cggattgtcg aaaatggtat aaaccggatg tgatcgttga tgctattatt gcaaagaaaa
    13981 ataccggaac taagattacg gacgctcctt ttgtgatcgg cataggtccg ggatttacag
    14041 cgggaaaaga ctgtcattgt gtgatcgaga caaaaagagg tcatactctc ggttcggtaa
    14101 tatgggaagg aagcgctatt gcggatacag gagttccggg aaacatagga ggatatacga
    14161 ccgagcgtct cattcgtgct tcggaagacg ggataataga accgaaagtc cgaatcggcg
    14221 atatagtcga aaaagggcag attgttgcag taaccggagg aaaaccggtt ttcgcccaaa
    14281 tgggcgggat cgtgagagga atgctgcagc cgggggcgta tgtgaagaag aatctgaaaa
    14341 tcggagatat agatgcccgc gcggagcgtt cccactgcga gacgatttcg gacaaggcaa
    14401 gagcaatagg aggcggagcg cttgaagcgg cagagcgctt tgaaaaaata aaagggcatt
    14461 atgcagtcgt tatattggcg gcgggaaagg gaacaagatt ctcaggggaa cgtggggaaa
    14521 gcaaacttca cgcaaagata gatcaaaagc cgatgtatat gcatatgatg gataaaatgc
    14581 aggctttttc atccgttgaa ctgtttattg ttacagggga tgagaagatt atagaggaag
    14641 ccgaaaaaag aggaatcttt gttgtgagaa ataaagagcc ggaaaaaggc atcgcacatt
    14701 ctgtcgtact tggcttaaag gcagtatctc atagttttaa gagcgggaca gacagaaaat
    14761 cgggtgcacc ggacggaatc ctgttttcag tgtgcgatca gccgaaactg aaaatatcca
    14821 caatccaaag aatattaaac gatgcggcgc tgcataagaa aaacgttatt tgtgcgggat
    14881 atcggggaca aaaaggaaat ccggttttgt ggccggctca ctgttttgcg gaattattag
    14941 aattaacagg ggatgaaggc ggcagacaaa tgcttacaaa atacgaagaa aaggtacgta
    15001 tcatagagac aagccgggaa gaactcaaag atatcgacag caagagggat ctggagaatc
    15061 tggggaaaga aacggaagaa aataaaaaag cgtgaataac tatccataca atagtacgtt
    15121 tctttttatt cattttagtc tatc
//
LOCUS       DBMDXF010000024        14924 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1186_length_14924_cov_1.744451, whole genome shotgun sequence.
ACCESSION   DBMDXF010000024 DBMDXF010000000
VERSION     DBMDXF010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14924)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_14924_cov_1.744451"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="ACZPD4_01935"
     CDS             complement(<1..1262)
                     /locus_tag="ACZPD4_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377872.1"
                     /translation="MMMFVIFLIVNVGTIGIFYGVYGTKRKYEEGMLMGVHLPGSAAE
                     SEEVKMLMKKYRTWTKWFYTLNLLAGIAVCGFCFWYISVFMIVWSIWLVEVCIGAILL
                     LYRTHRKLYDLKMKNGWIGSNGSKIMTADSAVDATVSAQSEKMGMSPLWHILFCGLIV
                     LPCLLPQVRLYLKESEDGWILFLCGFAVNLVFAAVHAVILRTRNTVYSEDVKMNLAVN
                     RLQKNVWSWSLIAGSLFNTGAYLWVTYFMDEKMWIGSEVYIVYIILESVPIFIFMSGV
                     FYIKKKRNRILQNNETLHYVDDDIYWKNGWYSNPNDKRLIVQDWACSFNYTTNMARPA
                     GKISFAAGIVITIVCLLWVCVLMFRIDFTPIKVSVGSDKIEITSGYSDMEIDIDEVQS
                     AELLPKLPNDEYKRVNGSDDGQKKIGKF"
     gene            1489..1890
                     /locus_tag="ACZPD4_01940"
     CDS             1489..1890
                     /locus_tag="ACZPD4_01940"
                     /inference="COORDINATES: protein motif:HMM:NF022281.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR1377873.1"
                     /translation="MKKRQNISALFTIAVFYLILESVFGITCPILFLTGISCAGCGMS
                     RAWFCVLRMDFTGAFRYHPLFWILPIGIMLFLFWDKIPKKIQKILLITACVLFGAVYI
                     LRMLDTADSIVVFQPEKGAIFSLISQLFTKK"
     gene            1913..3382
                     /locus_tag="ACZPD4_01945"
     CDS             1913..3382
                     /locus_tag="ACZPD4_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HIR1377874.1"
                     /translation="MQCKTCGREIEDTAKFCPYCGSKTEPPHNSAENKTAETAYETTA
                     NTNAETAYGTETNKNSETAYGTEANKNAETAYGTKTSKTPETDRETFKNNTEDRFQTA
                     SAGDLDETKKFLQIGFFILAALCFVMAFRNLLSGIQNFSYSLIMQGLGNLLNMVCYAG
                     IAAVLVMIALSKEKQRIKTFFVILSGLAAIIALCGIIEFITSMFRSFRYYFSFDTFFW
                     ILSSLLLTLTIMAAVVGITYTLLHLSGSTPDLSNITGVIGGSARSSQTSETAQGNTNK
                     KTNYQNAGTDHMNQNSKTNCESQAGSTNYEGQNMGTNYNNQGTNYNNQGPNYNNQGTN
                     YNNQGPNYNYQSSGPNYSGSGVTPLKTDRGLLGYIVLSILTCGIYSYYFIYTVARDVN
                     TACAGDGKSTGGLVQFILLSILTCGIYSWWWQYSLGNRLAANAPRYGLSFTENGTTIL
                     LWLFFGSFLCFIGPFIAMNIIIKNTNSICMAYNRYNGLI"
     gene            complement(3581..4528)
                     /locus_tag="ACZPD4_01950"
     CDS             complement(3581..4528)
                     /locus_tag="ACZPD4_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1377875.1"
                     /translation="MRRKVFRYSFLCALLFVFVTAVFMFTACGKEETVKEKEENKKEN
                     KEEIVIKKDDIEDTEEVGSSDPELEEEAAFPILNDLIDRIQLTAAPLESTGASVSVYA
                     ENLSDGARASVNSSQMPAASLIKLYIAGCVYEQMDFVKEQEVYAGETEELLRLMITVS
                     DNDAANELVRKLGFGDASAGMEVVNEFCRLHGFADTHMGRLLLAPNDMDDNYTSVNDC
                     GKFLREMNGNVLVGSEKLLDLMRQQERTGKIPAGVPENIETANKTGELSDVENDAAII
                     FGENGAYTVCVMMSGLSDTSAARSVIREISAQIYEYMCP"
     gene            complement(4568..5446)
                     /locus_tag="ACZPD4_01955"
     CDS             complement(4568..5446)
                     /locus_tag="ACZPD4_01955"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1377876.1"
                     /translation="MKKGVTFFVFICLLVFDVSACGRENVSDPSEGKKASAENPKTTD
                     RSSEESEQTSEQTSEQTEASVSAEEHSGQTDIQKPVKKDILIAIDPGHQSPDIDMSGQ
                     EPNAPGSGDFKQKSAGGTVGRFTGIPEYELNLEIALMLRDRLTEQGYDVILTRENNET
                     AISNAERACLANDAGAEFSLRIHANGSEDPSVNGAMALIGSAENPYVGGVYEESYRLA
                     EMILNCYCSASGMQNLGIRCNDTMTGINWSQIPVVILEMGFMTNQQDDTNMSDATYRE
                     LMVDGIVDGINAYYGF"
     gene            complement(5466..6695)
                     /locus_tag="ACZPD4_01960"
     CDS             complement(5466..6695)
                     /locus_tag="ACZPD4_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HIR1377877.1"
                     /translation="MRCQYCGRKIPDDSAFCPECGQRQEELSFHAESSFDSTQESKPK
                     VCPVCGTVMEEGTLFCPECGTPYARSEQQSGTRFEDTEEMPYISSPEDSEELLKVRDE
                     EEFEEFGSQRDGRGDMGNQGNVPDMRQSLDESYGKKKSKAPIIIGVVIGGIVLVAIVI
                     AVLFFVLKKEDPKPSSDSVNTDITEPSENEILNDVDYNLLDEDELFFEGFIKKTENGD
                     YVLRLEEEYTFYGEDFYGEKVLLEDARNVYIDQSVLPEGMLDSIKSNQTIEIEGQFYF
                     DNDKLYITPFVIWDEYGENLIEDFEKSKEEDEVLNKDYILPQSASYLLTESDIEGLDI
                     REINYAKNEIYARHGRLFQSAELQNYFNSKKWYHGTVSPEEFNNSMLSEIERKNADFL
                     AQVEFGMDPKGYQLDAE"
     gene            6890..7672
                     /locus_tag="ACZPD4_01965"
     CDS             6890..7672
                     /locus_tag="ACZPD4_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848102.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HIR1377878.1"
                     /translation="MKMNELKLIYFSPTGTTHKVVVETARSIDLKSVSFDLTIKKDLK
                     PVAKFAPNDFALFAIPVYYGRVPALFLKYLDLFSGNGTPAALVATYGCREYEDALLEL
                     KTEVEKRGFKVIGAAAFPAEHSIVNSIGARRPNKADLKEASEYGISLNRIIKKEDSFA
                     SFDLKVPGNTPYRKYGKNVLFPKADVSLCTECSACAKICPAGAISLDNPKKTDHDKCI
                     GCMKCVRFCKQKARYVSAFKLNMAAKKLTKVCQSDKKAEIFL"
     gene            complement(7881..13949)
                     /locus_tag="ACZPD4_01970"
     CDS             complement(7881..13949)
                     /locus_tag="ACZPD4_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HIR1377879.1"
                     /translation="MAGRVIKRLLAGVLTVAVVIPGNFVPAQAAEEPQEDYLIYPNPH
                     KVEYQEGDYILGKELNVIYDKGIDEATKNRLQEAADLKGIEVNEAEQPKEGATNVYVG
                     VHGQDGTAEDYITEEYQPEDSLFGKTDSYFLASDENVISVLGKDADSAFYGLTTLYHV
                     FAQMDSLTIRNFEIEDYADVVSRGFIEGYYGNPWSTEDRVNLMKWGGYYKLNAYFYAP
                     KDDPKHRTQWDQLYTEEELANKIRPLAEAGNESKCRFVYALHPFPQGNHLRFDDNYEA
                     DLAKLQAKFKQVIDQGVRQIAILADDFWNPGGPNGVRLLNDMTAWLEEVKKQYPDMKM
                     TIPYVPYDYMGNGSSAELQELKKAPANVQIVMTGGRVWGEVTNNFTSTFTNNVGRGPF
                     MWINWPCTDNSKKHLIMGGYDTFLHPGVEPSKIQGIMLNPMQQSEPSKVAIFGNADYA
                     WNIWETKEEADKSWNDAFSFVDHNSAIMNDASDALRELSKHMINQAMDTRVTPLQESV
                     VLKEKLNAFKEKLEAETLTAEEVDAVIAEFEVLQKASKTYRAGGNEAIKGQIVYWLNC
                     WDDTTEAAIAYLNGVKSALAGDVSSVISYNTAGKTAFDRSKTYDFLYVDHQEYAEVGV
                     QHIVPFINTLAEYVSAKAETAVNPDKVIPKFITSRKDAPTGSKENIFDGDESTSAVYK
                     TPNSLSKDDYIGVEYNKVIDIDSIRFLLGGGKDHFEHAKLQYMTEEGTWEDLTLTGME
                     NNFAGEFGKVQDINVKEANLPSDLKAKGIRLIATAANVNDCWLEVREIQINKKEEVSE
                     DTERYTGDVTFSGISTQGNDHTAAKMFDGDLSTEMWIAKGPYSGEGKDTIAADAYIQI
                     TFPEAKQIGSIRMTQGKTSSADVFKKVEIQYSTDGQSGWKKAGEFTNAKDQTVNINVT
                     DKIKGIRLVNKEQTAGWVRIGELDIRAPKNMTTPITYKVIKTERWKVAQGKETSLYDG
                     DDNTFVWYDPDGDGNSTSDSVLKDDFLGYDLGTEAVLESAHIVVGHTGGDKLVKYAVE
                     TSVDNSTWTPAEGYAEYTGAAEGKDVLNIDLKGVTARYIRIRNLEKKPTWAKFSEFTV
                     KQKVDQTGTTENIYTNVKDHGMLGTTEAGMSSLQPGTISLKKDQYVGVDLKNIKAIEN
                     IAVNAGENANVKLQSSMNGVIWTDVNAGALEDARYVRLYNAGNETQNVSVQEFKVTYA
                     FIGEKTVESDFAQKDSANDMRSNGQVGNVFDGKLNTLGKITGTQDAGKKIVFDLGQTV
                     NFESFRYYVKETCLDFLRHAKFEVADRKDATDDQWTKILEVGNAEAVQMSANATAKDA
                     DYLLHDTTNPGNMYAEATGLNVSGRYLRIVPLTTYTERWVELYELQINGGAYMTTESN
                     RDIISETAEEAGKIPSNVFDGNFATTYKPSAANGSFTYRISEPDQRTIRIIQNGKASN
                     ADVQAVLYKDGAKQEAAAIGKLNQTINEFAVGKDSQILEVIVTWAEDIPEISIIKTSE
                     KEKAAVNKDALNEAIKKPIDDKWTADSKRAAEEAKAAAEEIAANEYVTQEVVDLAEKA
                     LLAAHKNAVVKGDVTALENALNNMKAGKENVGAEEAPVYVEIYSARTYAAYESVMNEI
                     REALKDKENVSEKEAQDLTAKLEKAEKALVYSSIQRELAETELENAVKYNKDDYTTVS
                     YKAYTDAKSALDAIVKADKTERKNPKEVYTARNTFAQTEEGLVNVAALKAKLAEVGKL
                     DENLYTKDSWKELQDAVKNAEAYLENGTKDQVDKAVSDLENAVNGLVEKTEVTVDDVI
                     AEMEKINGKDYTEVSFGALQDAISKAKADKDQNDPALDQANITAMKEAKAALVSIVDL
                     KAALNEAAQHKAGTYTVSSYKLLKDAVTNAEPLKVNGTKEEVANAAAAIRAAIKGLDK
                     RAVGLDEYRDSIVLKKPEGYTEESYAAYKNAYDALMALDSKETTAEMFANAKSAFEAA
                     QAGLVQKPGSNGTGSSSNGTVSNGAVATGDTVNVSALAGLFLSLLMIAAFMKRKTFLG
                     FFEKDE"
     gene            complement(14207..>14924)
                     /locus_tag="ACZPD4_01975"
     CDS             complement(14207..>14924)
                     /locus_tag="ACZPD4_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1377880.1"
                     /translation="SYSGRCLLSNYFTGRDANRGACTHPCRWKYAVVEEKRPGEYLPV
                     YENERGTYIFNSKDLCMIEHIPELIDAGIDSLKIEGRMKTALYVATVARTYRKAIDDY
                     KKDPKLYEQNMPWYKEQISNCTYRQFTTGFFFGKPDETTQIYDSNTYNKEYTYLGIVG
                     EIKDGLCRIEQRNKFSVGETIEIMKPDGRNIEAEVLRILNEEGKEQESAPHSKQLLYV
                     ELSEIPDVYDILRRKEEESK"
BASE COUNT         3897 a         3756 c         2510 g         4761 t
ORIGIN      
        1 ctgaatttcc cgattttctt ttgaccatca tcagatccat ttactcgttt gtattcatca
       61 ttcggaagct ttggtaaaag ttctgcgctt tgcacttcat caatatcaat ttccatatcg
      121 gaatagccgg atgtaatctc gattttatca gatccgacag aaacttttat cggagtaaaa
      181 tcaatccgaa acatcagcac acacacccac agaagacata ctatcgtaat gactatccct
      241 gccgcaaacg aaattttccc tgccggacgc gccatattcg tcgtatagtt aaaagaacac
      301 gcccaatcct gcacgatcag ccgtttatca ttcggattac tgtaccatcc gtttttccaa
      361 taaatatcat catcaacata atgcaaagtt tcattattct gcagaatccg gtttcttttt
      421 ttctttatat aaaatactcc gctcataaaa atgaaaatcg gaacactttc aagtataatg
      481 tatacaatat atacttctga tccgatccac atcttctcat ccataaaata tgttacccaa
      541 agatacgctc cggtattaaa aagacttccg gctatcagtg accatgacca aacatttttc
      601 tgcaaccggt tcaccgccag attcatctta acatcttcac tgtacaccgt attgcgcgtt
      661 cgcaatataa ccgcatgtac agcagcaaac acaagattaa ctgcaaatcc gcacaaaaat
      721 aagatccatc catcttccga ctcttttaaa tatagtctca cttgcggtaa taaacatggc
      781 aggacgatca atccgcaaaa caaaatatgc cacaacggag acattcccat tttctccgat
      841 tgtgcagaca ctgttgcatc aactgccgaa tccgcagtca taatcttgct tccattactc
      901 ccgatccaac cgtttttcat cttcagatca tacagttttc tgtgcgttct atataataga
      961 agaatcgctc cgatgcacac ctccacaagc catatactcc acacgatcat aaagaccgaa
     1021 atataccaga agcaaaatcc gcataccgca attcctgcca gcaagttcaa agtataaaac
     1081 cattttgtcc atgtacggta ttttttcata agcattttta cttcttcgct ttcagccgcc
     1141 gaacccggca aatgcactcc cataagcatc ccttcctcat acttacgttt cgttccgtaa
     1201 acaccgtaaa aaataccgat tgtacctaca ttcacgatca aaaaaataac aaacatcatc
     1261 atctgtcatc ccgccgttcc tgcaaagttc cgatcataca aaccggcaag atcttccctt
     1321 tttttgatac gatctcatac tttattttgt attatctaca ataatacaaa atatgaggaa
     1381 aatcaacttc ttccaaagcc atatcctgat tgtgcgtcca caattatgta cacagattga
     1441 caatattttt cacctattgt aaactagaaa aataagggag aaaccaatat gaaaaaacgg
     1501 cagaatataa gcgcactttt tacaatcgca gtgttttatc ttattttaga atcggtattt
     1561 ggaattacct gtccgattct tttcctgacc ggaatttcct gtgccggctg cggaatgtcg
     1621 agagcatggt tttgtgtact ccgtatggac ttcaccggag ctttccgata ccatccgctt
     1681 ttttggatat taccgatagg aattatgctg tttctctttt gggataagat accaaaaaag
     1741 atacagaaga tcttacttat aactgcctgt gtactatttg gggcagtata catcttacga
     1801 atgttggata cggccgattc catcgttgtc tttcagccgg aaaagggagc gattttttca
     1861 ttgatttctc aactctttac aaaaaaataa aggaaacgag gagggctttt atatgcaatg
     1921 caagacatgc ggaagagaaa tcgaagacac cgcaaagttt tgtccgtatt gcggcagcaa
     1981 aactgagccg ccgcataatt ctgctgaaaa caaaacagca gaaacagctt atgaaaccac
     2041 tgcgaacaca aacgccgaaa cagcttacgg tacagaaaca aacaaaaact cagaaacagc
     2101 ttacggtaca gaagcaaaca aaaacgccga aacagcttac ggtacaaaaa caagcaaaac
     2161 accagaaaca gacagagaaa cttttaaaaa caatacagaa gaccgctttc aaacagcatc
     2221 agcaggcgac ttagatgaga cgaagaaatt tttacagatc ggctttttca ttttagcagc
     2281 actctgcttc gttatggctt tccgcaatct actctcaggc attcagaatt tttcttacag
     2341 cctcatcatg caaggtctgg gaaatcttct taacatggta tgttatgcag gaatagcggc
     2401 agtattggta atgatcgcac tttcaaaaga aaaacagagg atcaaaacat ttttcgtaat
     2461 cttaagcgga ttagcggcaa tcatagcgct gtgcggaatc attgaattta ttacatctat
     2521 gttccgaagc ttcagatatt atttttcttt tgatacattt ttctggatct tatcttcttt
     2581 attattaacg ttaaccatta tggcggcggt tgtcggcatc acttatacac tcctgcatct
     2641 ctcaggatcg acaccggatt tatcaaatat cacaggagtc atcggcggat ctgcccgcag
     2701 cagtcagaca tccgaaacag cacagggcaa tacaaataaa aaaaccaact atcaaaacgc
     2761 cggaacagat catatgaatc aaaacagcaa gacaaactgc gaatcacaag ccggcagtac
     2821 aaactatgag gggcagaaca tgggtacaaa ctacaacaat caaggaacaa actacaataa
     2881 ccagggacca aactacaaca accagggtac aaactataat aaccagggac caaattacaa
     2941 ctatcaaagt tccggtccga actattcagg atcgggtgtt acgccactga aaaccgaccg
     3001 cggactgctc ggttatatcg ttctttcgat tcttacatgc ggaatttaca gttattactt
     3061 tatatacaca gttgcccgcg atgttaatac tgcttgtgcc ggagacggaa aatcaacagg
     3121 cggattagta cagttcatcc tgctttccat cctcacttgc ggaatttaca gttggtggtg
     3181 gcagtattct ctcggaaacc gccttgcagc aaacgcacct cgttacggtc tgtcatttac
     3241 agaaaacggt actactattt tattatggct tttctttggc tctttcctct gtttcatcgg
     3301 accgtttatt gcaatgaata tcattattaa aaatacaaac agcatctgta tggcatacaa
     3361 tcgttacaac ggattaattt aatcatttaa cattataata aaaggttctg cccgaacgat
     3421 ctgcatcctg aaacttcaga tacaagtcaa acataaaatt cggtcagaac cttttttatt
     3481 tctcctatga tcttttcaca atctctttac aatctctttt taccatttcc taatatatat
     3541 ccgaaaataa tatcccgcag tcaaaatcaa aatattcctg tcacggacac atatactcat
     3601 aaatctgcgc cgaaatctct cggataacag atcttgcggc agaagtatca gataaaccgc
     3661 tcatcatcac acaaactgta tatgctccgt tttccccaaa tataattgcc gcgtcatttt
     3721 ccacatcgga aagttccccc gtcttatttg cagtctcaat attttccggc actcctgccg
     3781 ggatcttccc cgtacgttcc tgctgccgca tcagatcaag aagtttttct gatccaacca
     3841 gcacatttcc gttcatttcc cgcagaaact ttccgcagtc gttgacagaa gtataattat
     3901 catccatatc attcggtgca agaagcagcc ttcccatatg agtatctgca aaaccgtgca
     3961 accgacaaaa ctcattcaca acttccattc ccgccgaagc atccccaaaa ccaagcttac
     4021 gaacaagctc atttgccgcg tcattgtcgc ttaccgtaat catcagacgc aaaagttctt
     4081 ctgtttctcc tgcgtaaacc tcctgttctt tcacaaaatc catctgttcg tatacacatc
     4141 ccgcaatata aagcttgatc aaacttgccg ctggcatctg actgctgttg accgatgccc
     4201 tcgctccgtc agaaagattt tctgcatata cagaaaccga agctccggta ctttcaagcg
     4261 gcgccgccgt caactgtatc cgatcaatta agtcatttaa aataggaaac gcggcttcct
     4321 cttctagttc cggatcggat gatccgactt cttctgtatc ttcaatatca tcttttttaa
     4381 tcacaatctc ttccttgttt tcttttttgt tctcttcctt ttcttttaca gtttcttcct
     4441 tgccgcaggc agtgaacata aaaaccgccg tcacaaaaac aaacaagagc gcgcataaga
     4501 aactgtatct gaaaactttt cgcctcatac ttatttttac tccgtcacat aaaaatatct
     4561 aaagaactca aaacccgtaa tatgcattga tcccatctac aatcccatcg accatcaact
     4621 ctcgataggt ggcatccgac atgtttgtat catcttgttg attcgtcata aatcccattt
     4681 ctaatatcac aaccggtatc tgactccagt tgatccctgt catcgtatca ttacaccgaa
     4741 tcccgagatt ctgcattcca ctcgcactgc aataacaatt taaaatcatt tccgccaaac
     4801 gataactctc ttcgtaaact ccgcctacat aaggattttc agcagaaccg atcagagcca
     4861 tcgcaccgtt taccgatgga tcttcacttc catttgcatg aattctcaaa gaaaactccg
     4921 cccctgcatc atttgcaaga caggctcgtt ccgcattgct tatagctgtt tcgttatttt
     4981 cccgcgtcag gattacatca tacccttgtt ccgtcagacg atctcgaagc atcaatgcga
     5041 tttcaagatt aagttcatat tccggtatcc cggtgaatct gccgacagta ccgccggcag
     5101 atttttgttt aaaatcacct gacccaggag catttggctc ttgcccgctc atatctatat
     5161 ccgggctttg atgcccggga tctatcgcga tcaaaatatc tttttttacc ggtttttgta
     5221 tgtcagtttg tcctgaatgt tcctctgcgc tcacagacgc ctccgtttgt tccgatgttt
     5281 gttccgatgt ttgttccgat tcttctgatg accgatcagt agttttggga ttctcagccg
     5341 aagctttctt tccttctgaa ggatcggaaa cattttccct gccgcacgcc gaaacatcga
     5401 aaacaagaag acagataaat acaaaaaatg taaccccttt tttcataatt atgctccttt
     5461 aacaattact ctgcatccaa ctgatatcct ttcggatcca taccaaactc gacctgagcc
     5521 agaaaatctg catttttccg ttcaatttca gaaagcatcg aattgttaaa ttcttccgga
     5581 ctgaccgtac cgtgatacca tttcttacta ttaaaataat tctgcagttc agccgattgg
     5641 aaaagccggc catgcctcgc atagatttca tttttggcat aattaatctc gcggatatcc
     5701 aagccttcaa tatcgctttc agttaaaaga taagaagcgc tctgcggaag aatataatct
     5761 ttatttaaaa cttcatcctc ctcttttgat ttttcaaaat cttctatcag gttttcccca
     5821 tattcatccc aaattacaaa cggtgttata taaagcttat cgttgtcaaa ataaaattgt
     5881 ccttctatct ctatcgtctg attagattta atgctgtcga gcattccttc cggaagaaca
     5941 gattgatcga tatatacgtt tctcgcatct tccagcaaaa ctttttcacc gtaaaaatct
     6001 tctccgtaaa aagtatattc ttcttccaag cgcagaacat aatcgccatt ttctgtcttt
     6061 ttaataaagc cttcaaagaa taactcatct tcatcaagca ggttataatc tacatcattc
     6121 aaaatctcat tttccgacgg ttctgtaata tccgtgttga cggaatcaga agacggtttc
     6181 ggatcttctt ttttcaaaac gaaaaacaac acagcaatca ctatagcaac taaaacaatt
     6241 cctccgatga ccacaccgat tataatcggc gctttgctct ttttcttccc ataagactca
     6301 tccaaagact gccgcatatc cggcacattt ccttgattgc ccatgtcacc ccgaccgtct
     6361 ctttgtgatc cgaactcttc aaactcttct tcatcccgca cctttaacaa ctcttcagag
     6421 tcttccggag acgatatata cggcatttcc tcagtatcct caaaccgcgt accactctgc
     6481 tgctctgacc gtgcataagg cgtaccacat tcagggcaaa acaaagttcc ctcttccatc
     6541 acagtaccgc ataccggaca aactttcggc tttgattcct gtgttgaatc aaaacttgac
     6601 tctgcatgaa acgatagctc ttcctgacgc tgtccacatt ccggacaaaa tgcagaatca
     6661 tcaggaattt ttcttccgca atattgacat ctcataatcg tattcctcct tataaataac
     6721 tgctgctata tttatagtat atttttccat ccttccggtc aaacaaaatt tacgaatggc
     6781 ggggactgaa ctattgcttt tgttcgatat tctatattta tttcttcgtg catttttatc
     6841 cgtattatga tatcattgta atattactta caaaggagtc tgttgattta tgaaaatgaa
     6901 cgaattaaaa cttatttatt tcagccctac aggaacgacc cataaagtcg ttgtggaaac
     6961 ggcaagaagt atcgatttaa aatcagtctc ttttgacctt acgatcaaaa aagatctgaa
     7021 acctgtcgca aaatttgcgc caaacgattt tgccctgttt gcgatccccg tatactacgg
     7081 tcgtgttccg gcgctctttc tcaaatatct tgatctcttt tccggaaacg gaacacctgc
     7141 cgctcttgtc gctacatacg gatgccgcga atacgaggat gcacttttag aattaaagac
     7201 cgaagtagaa aaaagaggat tcaaagtaat cggagctgcc gcatttccgg cagaacactc
     7261 tatcgtgaac tctatcggcg caagacgtcc gaacaaggcg gatttaaaag aagcttccga
     7321 atacggaatt tcactgaatc ggatcattaa aaaagaagat tctttcgctt ctttcgatct
     7381 gaaagtaccg ggaaatactc cttacagaaa atacggaaaa aacgtacttt ttccgaaagc
     7441 cgacgtttcc ctttgtacag aatgcagcgc ctgcgcaaag atctgtccgg caggagcaat
     7501 ctctcttgat aatccgaaaa agacagatca cgataaatgt atcggatgta tgaaatgtgt
     7561 ccgcttctgc aaacagaaag ccagatatgt cagcgctttt aagttgaata tggcagcaaa
     7621 gaaattaaca aaggtctgtc agagtgataa aaaggccgag atatttcttt aagctacacc
     7681 gtcatctgtt attcaaaaca gcggttcaaa aatatataaa cagatatcga tcggtcgtca
     7741 agtttccggg ctttcccgcc ctttggctta ccgccttcgc aaaagaatcg ccgaatccgg
     7801 ctttactgcc ggatccggcg attcttaact ccaggtcatc ttatatcttt taatattatc
     7861 atgtgaacct gttttaaatc ttattcatct ttttcaaaaa atcccagaaa agttttacgt
     7921 ttcataaatg ccgctatcat caagagtgac aggaataatc cggctaatgc tgaaacattc
     7981 actgtatctc cagttgcaac tgctccgttt gaaacagttc cgtttgaaga acttcctgtt
     8041 ccgttgcttc ccggtttctg tacaagacct gcctgtgcag cttcaaatgc agatttcgca
     8101 tttgcaaaca tttctgccgt tgtctccttt gaatccagag ccatcagcgc atcatatgca
     8161 tttttatatg ccgcatagct ttcttctgta taaccttccg gtttcttcaa tacgatagag
     8221 tctctgtact catccaaacc aactgcacgt ttgtccagac ccttgatcgc cgcacggatt
     8281 gcagccgccg cattggcaac ctcttctttt gtaccgttta ctttcaacgg ctctgcattt
     8341 gtcacagcat ctttcaaaag cttataagaa cttaccgtat aagtacctgc tttatgctga
     8401 gcagcttcat taagagcagc cttcaaatct acgatactta caagcgccgc tttcgcttct
     8461 ttcattgcag tgatattcgc ctgatctaat gccggatcgt tctgatcttt atctgctttt
     8521 gctttgctga tcgcatcctg aagtgcgccg aaacttactt cagtgtaatc tttgccgttt
     8581 attttttcca tttctgcaat gacatcatct acagttactt ctgttttttc taccagacca
     8641 ttcactgcat tttccagatc ggaaaccgct ttatctacct gatcttttgt tccgttttcc
     8701 agataagcct ctgcattctt cactgcatct tgaagctctt tccaactgtc tttcgtataa
     8761 agattctcat ccagttttcc aacttctgca agttttgctt tcaatgcggc tacattgaca
     8821 agaccttctt ctgtctgcgc aaaagtattt cgtgcagtat agacttcttt cgggttcttc
     8881 cgctctgtct tatctgcttt cacaatcgca tctaatgctg atttcgcgtc tgtatatgct
     8941 ttgtaagaaa cagttgtata gtcgtcttta ttatatttta ctgcgttttc taactctgtt
     9001 tctgccagct ctctctggat cgatgaatat actagtgctt tttcggcttt ttcaagtttt
     9061 gccgtcagat cttgcgcttc tttctccgaa acattttctt tatcctttaa cgcctctctg
     9121 atctcattca tcaccgactc atatgccgca taagttctcg ccgaatagat ctctacataa
     9181 acaggagctt cctctgctcc gacgttttct ttgcctgctt tcatgttgtt caaagcgttt
     9241 tccaatgcag taacatcgcc tttgacaacc gcgtttttgt gtgcggcaag aagcgctttt
     9301 tctgccagat ctacaacttc ctgagtcacg tactcatttg ccgcgatctc ttctgccgct
     9361 gcttttgctt cttctgccgc ccgtttgctg tctgcagtcc acttatcgtc gatcggcttt
     9421 ttgattgcct cattcagcgc atctttattg accgctgctt tttccttttc acttgtcttt
     9481 ataatagaaa tctccggaat gtcttctgcc catgtcacga taacttccag aatctgactg
     9541 tcttttccaa ccgcaaattc attgatcgtc tgattcagct ttccgatcgc agctgcttct
     9601 tgttttgctc cgtctttata gagaactgcc tgtacatctg cattcgacgc ctttccgttc
     9661 tgtatgatac ggatcgttct ctgatccggc tcactgatgc gatatgtaaa cgaaccgttt
     9721 gccgcagatg gtttgtatgt tgtcgcaaag tttccgtcaa atacattaga cgggatcttg
     9781 cccgcttctt ccgctgtttc tgatatgatg tcgcggtttg actctgtcgt catataagct
     9841 ccgccgttga tctgaagctc gtacagctca acccatctct ctgtatatgt tgtaagcggt
     9901 acgattctta agtaacgtcc cgacacattc aatccggttg cctctgcata catatttccc
     9961 ggatttgtag tatcatgaag cagatagtct gcatctttcg ctgtcgcatt agccgacatc
    10021 tgcacagcct ctgcgtttcc gacttcaagg atctttgtcc actgatcgtc tgtggcatct
    10081 ttcctgtcag ctacttcaaa ttttgcatgt cttaagaagt caagacatgt ctcttttaca
    10141 tagtatctga acgattcaaa attcacagtc tgtccgagat cgaatacgat ctttttccct
    10201 gcatcctgtg ttcctgtgat cttgccaagc gtattaagct ttccgtcgaa tacatttccg
    10261 acttgtccgt tgcttcgcat atcgtttgcc gaatctttct gagcaaaatc actttctacc
    10321 gttttctctc cgataaacgc atacgtcact ttgaattctt gaaccgaaac attttgtgtc
    10381 tcatttcccg cattgtaaag tcttacatat cttgcatctt ccaatgctcc tgcattaaca
    10441 tcagtccaga tcactccgtt catagagctt tgcagtttca cattcgcatt ttctcctgcg
    10501 ttcactgcaa tgttttcgat cgctttgatg ttctttaagt caacaccgac atactgatct
    10561 tttttcagac tgatcgttcc cggctgcaaa gaagacatac ctgcttctgt cgttccgagc
    10621 attccgtgat cctttacatt tgtataaata ttctctgttg ttcctgtctg atcaactttc
    10681 tgttttacag taaactcaga gaattttgcc cacgtaggtt tcttctccag attgcggata
    10741 cggatgtaac gcgccgtcac acctttcaga tcaatgttca gtacgtcttt tccttctgcc
    10801 gctccagtat actctgcata tccttctgcc ggagtccatg tgctgttatc tacagacgtc
    10861 tcaaccgcat attttacaag cttgtctccg cctgtatgtc cgactacgat atgagcgctt
    10921 tccaatacag cttctgttcc gaggtcatat cctaagaaat catctttcag aacagagtca
    10981 cttgttgaat ttccgtctcc atcaggatca taccatacga atgtattgtc atctccgtca
    11041 taaagactcg tttctttccc ttgcgctact ttccaacgct ccgtcttgat cactttgtat
    11101 gtgatcggag tcgtcatatt cttcggcgcg cggatatcta attcaccgat gcgtacccag
    11161 cctgccgtct gctctttatt cacaagacgg attcctttga tcttgtcagt tacgttaatg
    11221 tttactgtct gatctttcgc gttcgtaaac tctcctgcct ttttccaacc gctctgtccg
    11281 tctgtgctat actgtatttc tactttttta aacacatccg cagaagaagt tttgccctgt
    11341 gtcattctta tgcttccgat ctgcttcgct tccgggaagg taatctggat ataagcgtct
    11401 gccgcgatcg tatcttttcc ttcgccgctg tacggtcctt tagcgatcca catttctgta
    11461 gacagatctc cgtcaaacat ttttgccgcc gtatgatcat ttccctgtgt ggaaataccg
    11521 ctgaacgtca catctcctgt gtaacgctct gtatcttcgg aaacttcttc ttttttgttg
    11581 atctggattt cgcgtacctc aagccagcag tcatttacat ttgccgctgt cgcaatgagg
    11641 cggattcctt ttgctttcag atcactcgga agatttgctt cttttacatt gatgtcctga
    11701 actttaccga attctccggc aaaattgttt tccatgcctg tcaacgtcag atcttcccat
    11761 gttccttctt ctgtcatata ctgcagtttt gcatgttcaa aatgatcttt tccgcctccg
    11821 agaaggaaac gaatgctgtc aatatcgatt actttgttgt attctacacc gatataatca
    11881 tctttcgaca atgaattcgg cgttttgtaa actgcacttg tagactcgtc gccgtcaaaa
    11941 atattttctt tagaacctgt cggcgcgtct tttctgcttg taatgaattt tgggatcact
    12001 ttgtcgggat tgaccgctgt ttccgctttt gcagatacat actctgccaa tgtattgata
    12061 aacggaacaa tgtgctgtac gccgacctcg gcatattcct gatgatccac atataaaaag
    12121 tcatatgttt ttgaacggtc gaacgccgtc tttcctgctg tattatagct gatcacagaa
    12181 gatacgtctc ctgccagagc gctctttacg ccgtttaagt atgcgatcgc cgcttctgtc
    12241 gtgtcatccc agcagttcag ccaatatacg atctgacctt tgatcgcctc gtttccgcct
    12301 gcacgatatg tctttgaagc tttttgaagc acttcaaact ctgcgattac cgcatctact
    12361 tcttccgccg tgagagtctc tgcttccagt ttttctttaa atgcatttaa cttctctttc
    12421 agtacaacag attcctgcaa aggtgttaca cgcgtatcca tcgcctgatt gatcatatgc
    12481 tttgataatt cacgcagggc gtcgctcgca tcattcataa ttgccgaatt atgatctaca
    12541 aaggaaaatg catcattcca tgacttgtct gcctcttcct ttgtctccca aatattccat
    12601 gcataatctg cattaccgaa aatagcaacc ttgctcggtt ccgactgctg catcgggtta
    12661 agcatgattc cctggatttt agacggctct actcccggat gaaggaacgt atcataaccg
    12721 cccatgatca aatgtttctt agagttatcc gtacacggcc agttgatcca cataaacggt
    12781 ccgcgtccaa cgttatttgt aaacgtagaa gtaaagttat ttgttacttc tccccatact
    12841 cttccgccgg tcataacgat ctgaacatta gccggagcct ttttcaactc ctgaagttct
    12901 gcgctgcttc cgttgcccat atagtcatac ggaacataag ggatcgtcat cttcatatca
    12961 ggatactgct ttttcacctc ttcaagccat gcagtcatat cgtttaacag acgtactcca
    13021 ttcggtccgc ccggattcca aaagtcgtct gcaaggattg cgatctgacg tacgccctga
    13081 tcgatcacct gtttgaattt tgcctgaagt ttcgccaaat cagcttcata attatcatca
    13141 aaacgaagat gattgccctg cgggaacgga tgaagggcat aaacaaatcg gcattttgat
    13201 tcatttcccg cctctgcaag cggtctgatc ttatttgcca gctcttcttc cgtataaagc
    13261 tgatcccact gtgtacgatg tttcgggtcg tctttcggcg catagaaata cgcgtttaat
    13321 ttgtaatatc caccccattt catcaagttt acacgatctt ctgtagacca cggatttcca
    13381 taatagcctt caataaaacc acggcttact acgtccgcat agtcctcgat ctcaaagttt
    13441 ctgattgtca ggctgtccat ctgtgcaaaa acatgataca gcgtagtcaa tccataaaat
    13501 gcagaatctg catcttttcc aagtacggat attacattct catcacttgc cagaaagtaa
    13561 gaatccgtct ttccaaacaa agagtcttcc ggctgatatt cttctgtaat ataatcttct
    13621 gccgttccgt cctgtccatg tacaccgaca tatacattgg ttgcgccttc ttttggctgc
    13681 tctgcttcgt ttacctcgat tcctttgagg tctgccgcct cttgcagacg gttctttgtc
    13741 gcctcatcga ttcctttgtc atagattaca ttcaattctt ttccgagaat gtaatctcct
    13801 tcctgatact ccaccttatg tggattcgga taaatgaggt agtcttcttg cggttcttcc
    13861 gctgcctgcg ccggaacaaa attgcccggt atcacaacgg cgactgtaag aacccctgcg
    13921 agcagtcttt tgatcactct tcctgccata aaccttttcc ttccttttct cataatatta
    13981 tcaggatact accatctatc attatatcat tttttactca tttacgatat actttttttg
    14041 tttttttcat ctattttcat catatttact tccttttcta tcaatcattt tgtcatttca
    14101 ccgtcttttt gaacagtttc gctctcttat ttcctccatt acaaaacggc atagccaaag
    14161 atacgataaa cgcctttgtc tatgccgttt ctatttatat tttcttctat tttgattcct
    14221 cttcttttct tcgcaaaata tcatatacgt ccgggatttc acttagttca acatagagca
    14281 gctgtttcga gtggggcgca ctttcctgct cttttccctc ttcattcagg atgcgcagca
    14341 cttccgcttc tatgtttctg ccgtccggct tcataatttc aatcgtttcc ccgacagaaa
    14401 atttgtttct ctgctctatc ctgcacaatc cgtctttgat ctccccgaca atgccaagat
    14461 aagtatactc tttattgtat gtgttgctgt catagatctg tgtcgtctca tccggtttcc
    14521 caaagaaaaa tccggtcgta aactggcggt aagtacaatt tgagatctgt tctttatacc
    14581 agggcatgtt ctgttcatat aacttcggat ctttcttata gtcatcgatt gcttttcggt
    14641 acgtccttgc cactgtcgcc acatacagag cggttttcat tctgccttcg attttcagac
    14701 tgtcgatccc cgcatcgatc agctccggta tatgttcgat catacacagg tcttttgagt
    14761 taaaaatgta agttcctctt tcattttcat aaacaggaag gtactctccc ggtctttttt
    14821 cttccacgac cgcatatttc cagcggcacg gatgagtaca tgctcctctg ttcgcatctc
    14881 ttcccgtaaa ataattactc aaaagacatc ttcccgaata agag
//
LOCUS       DBMDXF010000025        14197 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1250_length_14197_cov_2.072219, whole genome shotgun sequence.
ACCESSION   DBMDXF010000025 DBMDXF010000000
VERSION     DBMDXF010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14197)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1250_length_14197_cov_2.072219"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="ACZPD4_01980"
     CDS             complement(<1..372)
                     /locus_tag="ACZPD4_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HIR1377881.1"
                     /translation="MKYIVILCDGMADEPLESLSGKTPLEAAKTLNMDRLAKNAEIGM
                     VQTVPKGMSPGSDTANLSVIGYDPKKYYSGRSPLEALSIGAQMGDLDVSFRCNLVTLT
                     EDEDCYEERTILDHSSGEISTE"
     gene            complement(392..1609)
                     /locus_tag="ACZPD4_01985"
     CDS             complement(392..1609)
                     /locus_tag="ACZPD4_01985"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1377882.1"
                     /translation="MISIAVLGYGTVGSGVVEVLEKNKDVINQRAGDEISVKYVLDLR
                     DFPGDPIQEKIVHDIDVIINDPEVQIVVEVMGGVEPAYTFVKRCLEAGKSVATSNKAL
                     VAKHGATLLSIAREREINFLFEASVGGGIPIIRALNSSLTADRIEEITGILNGTTNYM
                     LTKMFYEGAQYDEVLKEAQDNGFAERNPEADVEGYDACRKIAILSSLISGHQVDFEDI
                     YTEGITQITKEDMMYAKEMKMTIKLLASSKRDGDHLHAIVAPALLGNEHPLYSVNGVF
                     NAVFVNGNMLGDAMFYGSGAGKLPTASAVVADVVDEAKHLHRNIMTMWKNEKLELLPI
                     EDTEKRFFVRISGDEKMRREETEAAFGAVTFVRAEGLENEFGIITEVMTEGAYRKIAE
                     KFEGILHMIRVEE"
     gene            complement(1686..2805)
                     /gene="prfB"
                     /locus_tag="ACZPD4_01990"
     CDS             complement(join(1686..2729,2731..2805))
                     /gene="prfB"
                     /locus_tag="ACZPD4_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_147625391.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1377883.1"
                     /translation="MVLLDELKLRLSAYEEPLKDLRDSLDLASKKLRVEELEKRLEEP
                     GFWDDPEVSQQVMKELKGLQDSVKEIEKLYSDYEDMMLLIEMSEEEDSEETAEEIEEE
                     LQQFEETFEELRIGTLLTGPYDRDNAIVTLHAGAGGTESCDWTGMLYRMYTRWAEKKG
                     YSIEELDYLEGDVAGVKGVTFEVKGENAYGYLRSEKGVHRLVRISPFNSAGKRQTSFA
                     SCDVIPDIEEDIDIEINDDDLKIDTYRSSGAGGQHINKTSSAVRITHLPTGIVVQCQN
                     ERSQHMNKDKAMQMLKSKLYLMKQQEQAEKMSDIRGEVRDINFGNQIRSYVMQPYTMV
                     KDHRTNAEVSNVGGVMDGNIDPFINAYLSANTEQASEQ"
     gene            complement(2889..5465)
                     /gene="secA"
                     /locus_tag="ACZPD4_01995"
     CDS             complement(2889..5465)
                     /gene="secA"
                     /locus_tag="ACZPD4_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1377884.1"
                     /translation="MGIIEKIFGTHSHNELKRIYPIVDRIEALAPAMEALSDEELKGK
                     TKEFKDRLNEGETLDDILPEAYAVVREAAWRSIGMRHYRVQLIGGIILHQGRIAEMRT
                     GEGKTLVSTLPAYLNALSGKGVHIVTVNDYLAKRDAEWMGKVHEFLGLTVGVILNGMD
                     NKERRAAYDCDITYVTNNELGFDYLRDNMVIYKEQLVQRGLNFAIIDEVDSVLIDEAR
                     TPLIISGQSGKSTKLYEACDILARQLERGEASGEFSKMNAIMGEDIEETGDFIVNEKE
                     KAINLTEDGVKKVEKFFHIENLADPENLEIQHNIILALRAHNLMFKDQDYVVNAEGEV
                     MIVDEFTGRIMPGRRYSDGLHQAIEAKEHVKVRRESKTLATITFQNLFNKFEKKSGMT
                     GTALTEEKEFRDIYGMDVIEIPTNKPVQRKDLEDAVYKTKAEKYHAVVEEVKRAHATG
                     QPVLVGTITIEVSELLSGMLKKEGIKHNVLNAKYHEQEAEIVADAGVHGAVTIATNMA
                     GRGTDIKLDEDARAAGGLKIIGTERHESRRIDNQLRGRSGRQGDPGESRFYISLEDDL
                     LRLFGSDRLMGAFNALGVADGEQLEHKMLSNAIETAQKKLETNNFGIRKNLLEYDQVM
                     NEQREIIYGERRRVLDGESMRDTVYNMITEYVENITDRFASPEAAAEDWDVKGLELNL
                     HKIIPMMELPSEKECIDMRQKELKHLLKERAVKAYEVKEAEFPEAEQIRELERVVLLK
                     VIDARWMDHIDDMDQLRQGIGLQAYGQRDPLVEYKMTGYNMFGEMTNMIAETTIRTLF
                     NVRVEQKVEREEVAKVTGTNKDDTSVRAPKKREDKKVYPNDPCPCGSGKKYKQCCGRK
                     PV"
     gene            complement(5843..11197)
                     /locus_tag="ACZPD4_02000"
     CDS             complement(5843..11197)
                     /locus_tag="ACZPD4_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HIR1377885.1"
                     /translation="MQKKIRKIVSSILASCMVISAFAGIAPQKAEAKTTLVNVAVNGT
                     VSTQNGENGSYVIGNINDGDPKTSWQTQGTWPATAVIQLDKGRSISKVAVELGEDGDN
                     STGRTALVTVQYAQNGITTDLIDFGSKRMTVDSKEEFTADVPKSATHIYVTLSDPQNS
                     DGSQGAFWPSVQEVEVFEEQEEKVSDYNNIANQAKITTDGNENQSEGSANLVDGNDKT
                     LYKFHNEAQTSEKYIALNFDEARTMDAFRIAFEHVGDTDSVDFAFEYSILAKKAGDSD
                     YTKIADHQKANRTDNYAQVYKFNAAQYSEVKIVMHSCKTQGGTQNGWPAVAEFEVYGS
                     EIEVQDTGSIAFKKPVHTPSGKNTAKNITDGSKKTQWTGAYYPSYVDIDLEKNYNLDT
                     VEVYTPENGYSQYTIYTSMDGRDFDKLAEKTSKDSCDAEKGEVYQAEGKEARIVRVYM
                     EYNSASTAAVLNEVRVTGKESKSEVQKRPEINTVKYEESEYNVEITENDTKNEVYGII
                     ERRLGKAYKDWFKLELAENPKKNGYDYFELSMDGDQVKIKGNDGVSLATGLNHYLKYF
                     CQVNLSQVGDQADMPENKPVVTEKVFKETKAEVRYSYNYCTLSYSMAFWGEQEWRDEL
                     DWLALNGVNVVLDATAQEEVWRRFLGELGYSHEDIKDFIAGPAYYAWAYMANLSGFGG
                     PVHDSWFEERTELARKNQLIMRKLGMQPVLQGYSGMVPTNIHDYDKNAEVIEQGEWCS
                     FQRPTMLKTTSSTFEKYAKKFYQCQKEVYGDVSNYYATDPFHEGGITGGMNASDISEK
                     VLTEMITADKDAVWIIQSWQGNPTTALLNGLDRVEKGTDHALILDLYAEKDPHYDEGR
                     PGAEAYGDEEEFDKTPWLFCMLNNFGGRLGLHGHLDNLANNIPKVFNETKYIAGIGIT
                     PEASVNNPVLYDFLFETIWQDDASQKMEVIDLDTWLDDYATRRYGAESESANQAWDIL
                     KETVYKASLNGLGQGAPESVVNARPNLTIGAASTWGNAVISYEKGDLEEAAALLLADY
                     DKLKDSAGYQYDLANVLQQVLSNSAQEYQKGMSAAFSAKDLDSFKTYSEKFMSVIEDM
                     EKVTGTSEYFLLGRWVEQAKALANNADDFTKELYEFNAKALVTTWGSKNQAEKGGLKD
                     YSNRQWSGLIGDFYKARWQRWITARTNELSGTDYESNIDWFEWEWEWVRSNKEYPTTA
                     TPQDLKTLGAEILENYSVKNPAANDEFDLPTEGMTATAGSAQPQTGKEGPASYVLDKD
                     VSTLWHSKYEGDDQNNLWIDIALGESKTVNGLRILPRNGAVNGVIVEYRIEVSNDNGQ
                     SYHEVATGTWTNDSGWKWAQFDQTEATNVRLYAVRTLSDQAGKNFASAAEIRIMAPKK
                     EEPQPEQVNKQFLEFLIGYAQSAKEKPEYEHVVPEVKKALDAALAKAVQVKDNEKASQ
                     EQVDEAYTELLEIVHMLDFIGDTTSLKVLVDAVSDKTEKDYTPSTWQPFKSALDAANE
                     VLKNENALDADIEAARTALETAVNGLVKRADFTKLQASVDEAKKAYGDLSGYTEESAA
                     KYTQALTAAETVLKNADASQTEVDQAKTDLETAISGLTAKPSPDKVDKTALQSYVDFA
                     KKYADKEEEYTPSTWNAFKAAYDKAQEVLKDEKAAQKEVDDALAKLQKAAVDLEMKPS
                     KEKLEELKKAIKAAEKKDLSGYTDASVEAYKDVVEKAKTMLSDKETTATEVEDMLKTL
                     KDADKLLVKKDDVKEDEDKNPDSQKGQAVQTGDQTPIMFWAFMLVASAVVIFRRRKRA
                     "
     gene            complement(11546..12319)
                     /gene="hisA"
                     /locus_tag="ACZPD4_02005"
     CDS             complement(11546..12319)
                     /gene="hisA"
                     /locus_tag="ACZPD4_02005"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HIR1377886.1"
                     /translation="MKFRPCIDIHNGKVKQIVGGSLKDEGNMAKTNFASDLNAAFYAN
                     MYKEDCLRGGHIILLNSRQSEYYEQTKQQAKEALRVYPKGLQIGGGITDENAYEYIEC
                     GASHVIVTSYVFRGGEFCRENLKKLVHAVGKEHIVLDLSCRKQGEDYYVVTDRWQKYT
                     NLRLNAAVLEELSSYCDEFLIHGVDVEGKRSGMEEQLVHMLGQWGKIPITYAGGISSE
                     DDLERFRSLGGGQLDFTIGSALDLFGGTVSYRKIVNKYN"
     gene            complement(12443..12838)
                     /locus_tag="ACZPD4_02010"
     CDS             complement(12443..12838)
                     /locus_tag="ACZPD4_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377887.1"
                     /translation="MKKMKQLILLTLCVFTLFGMTACAGKDKTDNAADQTTTDKDRDN
                     DRNDASDKSDKKGDGILDDAVNDVTQGVDDVTDDVTDGIDDVTDDVTDGVDNAADDLT
                     DENGAKKEGDRMKNTDQGDATGNADDSMR"
     gene            complement(12927..13748)
                     /locus_tag="ACZPD4_02015"
     CDS             complement(12927..13748)
                     /locus_tag="ACZPD4_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1377888.1"
                     /translation="MGKEEIQESEPLRIYLNEIGEIPLLDETEEKELGRRISDGDESA
                     RARLEEGNLRLVVSIAKHYTGRGLPLMDLIQEGNIGLMHAAEKYDYTKDNRFSTYASW
                     WIKEAITRAIDQQSREIRVPVHVAENIKRVQKAAKELQQEFGREAEPGEIAKKLGDRT
                     EEEVKNILSYIRNPVSLETPVGEDGEDSLGDFVEDRSETTPEDAMDVLVRKEEVQELL
                     ETLNDREKEVISLRFGLGKDRTYTLEEIGESLGITRERVRQIESKAMEKLRKKAL"
     gene            complement(13814..>14197)
                     /locus_tag="ACZPD4_02020"
     CDS             complement(13814..>14197)
                     /locus_tag="ACZPD4_02020"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIR1377889.1"
                     /translation="DPAVSHIVNGHVPVKRKDGENPVKCGGKVLVIDGGFSKAYQKET
                     GIAGYTLIFNSYGLLLVAHEPFESTESAIAKEKDIHSETMIVKRVRERLLVGDTDIGE
                     ELKRQVKDLERLLVAYRNGELREKR"
BASE COUNT         3286 a         3843 c         2377 g         4691 t
ORIGIN      
        1 ctctgtcgat atttctcccg aactgtgatc aagaatcgtg cgttcttcat aacaatcttc
       61 atcctctgtc aatgtaacaa ggttgcaccg aaaagacaca tccaagtccc ccatctgcgc
      121 gccgatactc aaagcttcta acggagaacg tcctgaataa tacttcttcg gatcatatcc
      181 gatcaccgac aggtttgcag tatcgcttcc cggagacatt cctttcggaa ctgtctgaac
      241 catcccgatc tccgcgtttt ttgccagacg atccatattc agagtctttg ccgcttcaag
      301 cggtgttttc cctgacagag actccagtgg ttcatctgcc attccgtcac ataaaataac
      361 gatatatttc ataattttcg tcctctttat tttactcttc aacacggatc atatgaagga
      421 ttccctcaaa tttctccgcg atctttctgt atgctccctc tgtcatcact tctgttatga
      481 taccgaactc attttcaaga ccttccgctc tcacaaaagt cactgctcca aatgccgctt
      541 ctgtttcttc ccgacgcatt ttttcgtcgc cgctgatccg tacaaagaaa cgtttttccg
      601 tatcctctat cggaagaagt tccagctttt catttttcca cattgtcata atatttctgt
      661 gaagatgttt tgcttcatct acaacatccg ctacaacagc gctcgccgta ggaagttttc
      721 ctgctccgct tccgtaaaac attgcgtctc caagcatatt tccgtttaca aacactgcgt
      781 tgaacacacc gtttacgcta taaagcggat gttcatttcc aagaagcgcc ggagcaacga
      841 tcgcatggag gtggtcgccg tcacgcttac tcgatgcaag aagtttgatc gtcattttca
      901 tctcttttgc atacatcata tcttcttttg ttatctgcgt gataccttct gtgtaaatat
      961 cttcaaaatc aacctgatgt cctgaaataa gagaagaaag aatcgcgatt ttccggcatg
     1021 cgtcatatcc ttctacatca gcctcaggat tgcgctccgc aaagccgtta tcctgcgctt
     1081 ctttaagcac ttcgtcgtac tgagcacctt cataaaacat cttcgtaagc atatagtttg
     1141 tcgttccgtt caaaattccg gtaatctcct caatgcggtc tgccgtcagc gaagaattta
     1201 acgcgcggat aatcgggata cctcctccta cacttgcttc aaacaggaaa ttgatctccc
     1261 gctctcttgc gatagacaga agagtcgcgc catgctttgc cacaagcgct ttgttggatg
     1321 tcgccacgct ctttcccgct tccagacacc gttttacaaa tgtatacgcc ggttctactc
     1381 cacccattac ttccactacg atttgaactt ccggatcatt aatgatcaca tcaatgtcat
     1441 gcactatctt ttcctgtatc ggatctcccg gaaaatctct cagatcaaga acatatttga
     1501 cactgatttc atctcctgct ctctgattga taacatcctt atttttttcg agcacttcta
     1561 caactcccga accgactgtg ccatatccca ataccgctat acttatcatc ctattcttcc
     1621 tttcctatcc ctttttctgt tcgtaacatt tcacaaaacc actattagcc aaaacaggct
     1681 tccgatcact gctcggatgc ctgttctgta tttgcactta aatatgcgtt gataaacgga
     1741 tcgatatttc cgtccatcac tccgcctacg ttacttactt ccgcatttgt acggtgatct
     1801 ttcaccatcg tatacggctg catgacatag gaacggatct gatttccaaa attaatatcc
     1861 cttacttctc cgcggatatc agacattttt tccgcctgtt cctgttgctt catcaaataa
     1921 agctttgatt tgagcatctg catcgcttta tccttgttca tatgctgaga gcgttcattc
     1981 tgacactgaa ctacgattcc ggtcggaaga tgcgtaatac gcactgccga ggacgtctta
     2041 tttatatgct gacctcccgc gccgctggaa cgatatgtgt cgatcttcag atcatcgtca
     2101 ttgatctcta tatcaatatc ttcctctata tccggaatca catcacagga tgcaaatgat
     2161 gtctgacgct ttcctgccga attaaacgga gaaatacgca cgagacggtg tactcctttt
     2221 tccgagcgaa gatatccgta tgcattctcg cccttcacct caaaagtcac gccttttact
     2281 cctgccacat ctccctcaag ataatccagc tcttcgatac tgtacccttt cttttccgcc
     2341 cacctcgtat acatacggta gagcattccg gtccagtcac aagattctgt accgcccgcc
     2401 ccggcatgaa gcgtcacgat cgcattatct ctgtcatatg gtcctgtaag aagtgtcccg
     2461 atacgcaatt cttcaaacgt ctcttcaaac tgctgcaatt cttcttcaat ctcttccgct
     2521 gtttcctcgc tgtcttcttc ctcgctcatc tcaatcagaa gcatcatatc ctcataatct
     2581 gaatacagct tttcgatttc ttttacagag tcctgcagcc cttttaattc tttcattact
     2641 tgttgggaaa cttccggatc atcccaaaat cccggttctt ccaaacgctt ctccaactct
     2701 tcaacccgca gcttcttaga agctaagtca aagtgaatcc ctcaaatctt tcagcggttc
     2761 ttcatatgcg cttagtctta acttcagttc atctaacaaa accaccttga tcacctcttt
     2821 catttattat acaaatcctg tcttatcttt tattatacgg attctattgt tttttataat
     2881 accgtctatt acaccggttt tcttccacag cattgtttgt attttttccc gcttccgcaa
     2941 ggacatggat cgttcggata aactttctta tcttcccgct ttttcggagc gcgtacggaa
     3001 gtgtcatcct tatttgttcc tgtaactttc gctacctctt ctctttctac tttctgctcg
     3061 acacgaacat tgaacagtgt acggatcgtt gtctcggcga tcatatttgt catctcgcca
     3121 aacatattat accctgtcat cttatactca acaagcggat ctctctgtcc gtatgcctga
     3181 agaccgattc cctgacgaag ctgatccata tcgtcgatat gatccatcca tcttgcatcg
     3241 attactttta gaagtacgac acgctctaac tctcggatct gttctgcttc cgggaattcc
     3301 gcttctttca cttcatacgc tttcaccgca cgttctttca agagatgttt cagctctttt
     3361 tgacgcatat caatacattc tttctctgac ggaagctcca tcatcggaat aatcttatgc
     3421 aagttcagct ccagaccttt tacatcccag tcttctgctg ccgcttccgg agaggcgaaa
     3481 cggtctgtaa tattttcaac atattctgtg atcatattat agacggtatc tctcatgctc
     3541 tcgccgtcga gaacccttct tctctctccg taaatgatct ctctttgctc gttcataacc
     3601 tgatcatact caagcagatt tttacggata ccgaagttgt ttgtctccag ttttttctgt
     3661 gctgtctcta tcgcattgga aagcatctta tgctccaact gttccccgtc tgcaacaccc
     3721 agcgcattaa aagctcccat aagacggtcc gaaccgaaca gacgaagaag atcatcttcc
     3781 agtgaaatat aaaatcttga ttcacccgga tctccctgac gaccggaacg tcctctaagc
     3841 tgattgtcaa tacgtcttga ctcatgcctt tccgtaccga taatctttaa tccgcctgcc
     3901 gctctcgcat cttcatcaag tttaatatcc gtaccacgac ctgccatgtt cgtcgcgatc
     3961 gttacggcgc cgtgaacacc tgcatccgct acgatctctg cttcctgctc gtgatatttc
     4021 gcattcagaa cgttatgttt gattccttct tttttcagca taccgctcaa aagttcggac
     4081 acttctatcg tgatcgttcc gacaagaacc ggctgacctg tagcatgagc tctctttact
     4141 tcttcaacga cagcatgata tttttctgct tttgttttat ataccgcatc ttcaaggtct
     4201 tttctctgaa ccggtttgtt tgtcgggatt tctataacgt ccattccgta aatatcacgg
     4261 aactcttttt cttctgtaag agccgtacct gtcataccgc tcttcttctc aaatttatta
     4321 aacaagttct ggaatgtgat cgtcgcaagc gtcttgctct ctctgcgcac ttttacgtgt
     4381 tccttcgcct caatcgcctg atgcagcccg tccgaataac gtcttcccgg catgatacgt
     4441 cctgtaaatt catcgacgat cataacctct ccttctgcat tcacaacata gtcctgatct
     4501 ttaaacatca gattatgcgc gcgcaaagca aggataatat tatgctggat ctccagattt
     4561 tccggatcgg caagattttc aatatggaag aatttctcta ctttcttaac tccgtcttct
     4621 gtcaggttaa ttgccttttc cttttcattg acaataaaat cacccgtctc ttcgatatcc
     4681 tctcccataa tcgcattcat cttggagaat tctccgcttg cctcgccacg ttcaagctga
     4741 cgggcaagaa tatcacacgc ttcataaagc tttgtcgatt ttccgctctg cccggaaata
     4801 ataagcggcg ttctcgcctc gtcgatcaaa acagagtcaa cctcatcgat gatcgcaaaa
     4861 ttcagacctc tttgtacgag ctgttcttta tatatgacca tgttgtcacg cagatagtca
     4921 aatccgagtt cattattcgt tacataagta atatcgcagt catatgccgc acggcgctct
     4981 ttattgtcca ttccgtttaa aataacaccg actgtcaatc caagaaattc atgtactttt
     5041 cccatccatt cagcgtcacg ttttgccaga taatcattga ccgtaacgat atggacgcct
     5101 tttcctgaaa gtgcgttcag ataagccgga agtgtagaca caagcgtctt accttcacct
     5161 gtacgcatct cggcaatacg accctggtgc agaataatac caccgataag ctgtactctg
     5221 taatgacgca taccgatact tctccatgct gcctctctta caacggcata agcctcagga
     5281 agaatgtcgt caagcgtctc tccttcattc agtctgtcct taaactcctt tgtttttcct
     5341 ttcagttcct catctgacag cgcctccatc gcaggagcaa gagcttcgat ccggtctacg
     5401 atcggataaa ttcttttcag ctcattatgg ctgtgggttc cgaaaatctt ttctataata
     5461 cccatattct gtaattaact ccttatataa atttagcatc ggtaatagct ccgatgccac
     5521 cactattctt cattttaaca ctaaccaaag cgtaattcaa tactcaaaaa ttgttttgac
     5581 aaccatacgc gcaccgcctt ttacgcacaa aaacgatact cttatatcgg aaataatgat
     5641 cccattcaga atcattcaat caccggaagt accaaaaggc acattccggg agtgcaagcg
     5701 gtcgccaagg tcacgggcaa gcccggactt tggcttatcg ccattgcaaa aagcacagaa
     5761 cccattccaa aggctctgtg cttttctcat tcatccgcac acaatatttg attatcctgt
     5821 gctgattttt ttatgcattg ctctatgctc tttttcttct tctgaaaata acaactgcgg
     5881 aagctaccag catgaacgcc cagaacatga tcggtgtctg atcgcctgtc tgaactgcct
     5941 gacctttctg tgaatccgga tttttatcct catcttcctt aacgtcatct tttttcacaa
     6001 gaagcttgtc tgcatctttt aatgttttca gcatatcttc aacctctgtt gctgttgttt
     6061 ctttatctga caacattgtt tttgcttttt ccacaacatc tttatatgct tcgacagacg
     6121 catccgtata tccgcttaaa tctttctttt cagccgcttt gatcgctttc tttaattcct
     6181 ctaacttttc tttgctcggc ttcatttcca aatcaacggc agctttctgc agtttagcca
     6241 aagcgtcgtc tacctctttt tgtgccgcct tctcatcttt aagaacttcc tgcgctttat
     6301 catatgccgc cttaaaagca ttccatgtcg aaggagtata ctcttcttct ttatctgcgt
     6361 atttctttgc gaaatctaca taactctgaa gcgcagtctt atccacttta tccggactcg
     6421 gttttgcagt cagtccgctg atcgcagttt caagatccgt cttcgcctga tctacttctg
     6481 tctgtgaagc atccgcgttt ttaagtaccg tttcagccgc tgtcagcgct tgcgtatact
     6541 tcgctgcgga ttcttccgta tatcccgaaa gatccccgta cgcttttttc gcctcatcaa
     6601 ccgaagcctg cagttttgtg aaatctgcac gttttacaag tccgtttacc gctgtctcaa
     6661 gcgctgtgcg agccgcctcg atatccgcat caagcgcatt ttcatttttc aagacttcat
     6721 ttgccgcatc caatgcgctc ttaaacggct gccatgtgga cggagtatag tctttttcag
     6781 tcttatcgct cactgcatcg accaatactt tcaggcttgt cgtgtcacct ataaagtcaa
     6841 gcatatgtac gatttcaagc aattctgtgt acgcttcgtc aacctgctcc tgtgacgctt
     6901 tttcattatc tttcacctga actgctttcg caagcgctgc atctaacgct tttttcactt
     6961 caggaacgac atgttcatat tccggttttt ctttcgcgct ctgtgcgtat ccgatcaaaa
     7021 attcaaggaa ctgcttattt acctgttccg gctgcggttc ttcttttttc ggagccatta
     7081 tacggatttc tgccgcagat gcaaagttct ttcctgcctg atctgacaat gttctcactg
     7141 catataatct aacgtttgtc gcctctgtct gatcaaactg agcccatttc catcctgaat
     7201 catttgtcca tgttcctgtc gccacttcat gataagactg tccgttatca ttgcttacct
     7261 caatacggta ttcaacgatc acaccgttca ccgcgccgtt tcttggaagt atacgcagtc
     7321 cgttgacagt cttgctctct cccagggcaa tatcgatcca cagattattc tggtcgtcgc
     7381 cttcatattt actgtgccaa agcgttgaca cgtctttatc cagaacatat gatgcgggtc
     7441 cttctttccc tgtctgcggc tgcgcgcttc ctgccgtagc agtcatccct tctgtcggaa
     7501 ggtcaaactc atcatttgcc gccggatttt ttacagagta gttttctaaa atttctgctc
     7561 cgagtgtttt cagatcctgc ggtgtcgcag ttgtcggata ctctttattg ctgcgtaccc
     7621 attcccattc ccactcaaac cagtcgatat tactctcata atccgttccc gaaagctcgt
     7681 ttgttcttgc tgtgatccag cgctgccatc ttgctttata gaaatcaccg atcaagcctg
     7741 accactgacg gttagagtaa tctttcagac cgcctttttc cgcctgattt ttcgatcccc
     7801 atgtagtaac gagcgctttt gcattgaact catacagttc ttttgtaaag tcatccgcat
     7861 tatttgcaag cgcttttgcc tgctctaccc atcttccgag aaggaagtat tcgcttgttc
     7921 ccgttacttt ttccatgtct tcgatcactg acatgaattt ttccgaatat gttttaaagc
     7981 tgtccagatc ttttgcgctg aacgctgcag acataccttt ttgatattcc tgagcgctgt
     8041 tggaaagtac ctgctgcaaa acatttgcca gatcgtactg atatcctgcg ctgtctttca
     8101 gcttatcata atcagctaac agaagcgctg ccgcttcttc aagatcgcct ttttcatagc
     8161 tgatcaccgc gttgccccat gtagaagccg ccccgatcgt aagattcggt cttgcattta
     8221 caactgactc cggcgcaccc tgtccaaggc cgtttaagct cgccttatat actgtttctt
     8281 tcaaaatatc ccatgcctga tttgccgact cactctctgc tccgtatctt ctcgtcgcat
     8341 aatcatcaag ccatgtgtca agatcgatca cttccatctt ctgtgacgca tcatcctgcc
     8401 agatcgtctc gaacaagaaa tcatacaata ccgggttatt tacactcgcc tcaggagtga
     8461 ttccgattcc cgcgatatat ttcgtctcat taaatacttt cggaatatta tttgcaaggt
     8521 tgtcaagatg tccgtgcagt ccgagacgtc ctccgaagtt attcagcata cagaacagcc
     8581 acggagtctt gtcaaattct tcctcgtctc catatgcttc cgcgccggga cgtccttcat
     8641 cataatgcgg gtctttctct gcgtacaagt caagaatcag cgcatgatct gttccctttt
     8701 ccactctgtc aagaccgttt aacaatgcag ttgtcggatt accctgccat gactggatga
     8761 tccaaaccgc atctttgtct gccgtgatca tttcagttaa tactttttcc gaaatgtcgg
     8821 acgcattcat accgcctgta atgccgcctt cgtggaacgg gtctgtcgca taatagttgc
     8881 ttacgtctcc gtaaacttct ttctgacact gatagaattt cttcgcatat ttttcaaatg
     8941 tagaagaagt tgtctttaac atcgtcggac gctggaagga acaccactcg ccctgctcga
     9001 tcacttctgc atttttgtca taatcgtgaa tatttgtcgg aaccattccg ctgtaacctt
     9061 gcagtacagg ctgcattccg agttttctca taataagctg attctttctt gccagctctg
     9121 ttctctcttc aaaccacgag tcatgcaccg ggcctccgaa accggaaagg ttcgccatat
     9181 atgcccacgc ataatatgca ggtcctgcaa taaagtcttt aatatcttcg tgagaatatc
     9241 caagttctcc taaaaatctt ctccatactt cttcctgcgc tgtcgcgtca agaacgacgt
     9301 tcacgccgtt taacgccagc cagtctaact cgtcacgcca ttcctgctct ccccagaacg
     9361 ccatagaata agaaagcgta cagtagttat aagagtagcg gacttccgcc ttcgtctctt
     9421 taaatacttt ttctgtcaca acaggtttat tttccggcat atctgcctga tctcccactt
     9481 gtgaaagatt tacctgacag aaatatttca gataatggtt cagtcctgtc gcaagagaaa
     9541 caccgtcgtt tcctttgatt ttcacctgat ctccatccat ggaaagttca aagtaatcat
     9601 aaccgttctt tttcggattc tccgcaagtt caagcttaaa ccagtctttg taagcttttc
     9661 cgagtcttct ttcgataata ccgtaaactt cgtttttcgt atcattttct gtaatctcta
     9721 cattatattc tgactcttca tattttactg tattgatctc aggtcttttc tgaacttctg
     9781 acttgctctc ttttcctgtc acacggactt cgttcagtac agccgccgta gacgcggaat
     9841 tatattccat ataaacgcgg acgattctcg cctcttttcc ttccgcctgg taaacttctc
     9901 ccttttcagc atcacaggaa tcttttgatg tcttctctgc cagtttatca aaatctcttc
     9961 cgtccatact tgtatagatc gtatactgag aatatccatt ctccggtgta tatacttcta
    10021 ccgtatcaag gttataattt ttctccagat caatatctac ataagacgga taatatgcgc
    10081 cggtccactg tgtctttttg cttccgtccg taatattttt cgccgtattt tttccagacg
    10141 gcgtatgtac cggtttctta aatgcgatac tgcctgtatc ctgtacttcg atctcgcttc
    10201 cgtacacctc aaactctgcc actgccggcc atccgttctg cgttccgccc tgtgtcttac
    10261 aagaatgcat aacaatcttc acttctgaat actgcgcggc attaaactta taaacctgcg
    10321 cataattatc tgtacggttt gccttctggt gatctgcaat cttcgtataa tcgctgtctc
    10381 ctgctttctt cgccaggatg ctgtactcga acgcgaaatc aacactgtcc gtatctccca
    10441 catgctcaaa agcaatgcgg aacgcatcca tcgttcttgc ctcgtcaaaa ttcaaagcga
    10501 tgtacttctc tgaagtctgc gcttcattgt ggaacttata caatgttttg tcgtttccgt
    10561 caacaaggtt tgctgatcct tcagattgat tttcgtttcc gtctgttgtg atcttcgcct
    10621 gatttgcaat gttattgtaa tcagacactt tttcttcctg ctcttcaaac acttccactt
    10681 cctgaaccga cggccagaac gcgccctgac ttccatcaga attctgcgga tcggaaagcg
    10741 ttacataaat atgcgttgcc gacttcggca cgtccgctgt aaactcttct tttgaatcta
    10801 ccgtcattct cttgcttccg aaatcaatca aatccgttgt aatgccgttc tgtgcatact
    10861 gaactgtcac aagcgccgtt cttcctgtac tgttatcccc gtcttcgcca agttctacag
    10921 ccacttttga aatactgcgt cctttatcca actggatcac agccgttgca ggccacgttc
    10981 cttgtgtctg ccagcttgtt ttcggatcac cgtcattgat gtttccgatt acataacttc
    11041 cattctcacc gttctgcgta cttaccgtac cgttaacggc aacattgaca agcgttgtct
    11101 tcgcctctgc tttttgcgga gctatgcccg caaatgcgct aataaccatg caggaagcaa
    11161 gaatcgatga tacgatcttt ctaatcttct tttgcattct tccactcctt tatcaataat
    11221 atcagaccga agcggcagat ctgttttcga tgtcattgca gatttctgca taaaatgctg
    11281 cgcgaataca ggcagaaaaa aagatccgat attctccggc ataatgcata gaaaatatct
    11341 gatcttatct gttcaaagcc gccatctatg tctttgctct ttatcagaat agcaaatttt
    11401 ctcaaaaatg tcaatttata catttgtatt gcttgttgta catttgcacc gcttatatca
    11461 tgctcttttc tatctattaa taccaaacag acctttcaaa atataccatt ttttctcaaa
    11521 tagtcacctg ttatgcatct tttcatcagt tatatttgtt cacaattttt ctatatgaaa
    11581 cagttcctcc gaaaagatcg agggcgcttc ctatcgtaaa gtcaagttgt ccgccgccca
    11641 aactccgaaa ccgttccaaa tcgtcttccg aactgattcc tccggcgtat gtaatcggaa
    11701 tttttcccca ttgcccaagc atatgtacaa gttgttcttc cattcccgat cgttttccct
    11761 ctacatcaac gccgtggatc aaaaactcat cacaataaga agataattcc tcaagaacag
    11821 cggcgttcaa acgtagattc gtatattttt gccaacgatc tgtaactaca taataatctt
    11881 caccttgttt tcggcagctc aaatcgagca cgatatgctc ttttcctacg gcatgaacaa
    11941 gcttctttag attttctctg caaaattctc ctccgcgaaa cacatacgat gtgacgatca
    12001 catggctcgc tccgcattct atatattcat atgcattctc gtctgtaatc cctcctccta
    12061 tctgcagtcc tttcggataa acacgaagag cctctttcgc ctgttgtttt gtctgttcat
    12121 aatattctga ctgccggctg tttaaaagaa taatatgacc tcctctcaga caatcttcct
    12181 tatacatatt cgcataaaat gcggcgttta aatcagatgc gaaattcgtc tttgccatgt
    12241 tcccctcatc ttttaaactt ccaccgacaa tctgttttac ttttccgtta tgaatatcaa
    12301 tgcacggtct gaatttcatc tgcccttctc cttatgtatt gtttacacta ttacaagatc
    12361 gaccgcagac ataaagttcc ttctctctgc ggtcgaatca atcccgatta ttttataact
    12421 gttcttatca tgcgacaaaa aactatctca tagagtcatc ggcatttcct gttgcatctc
    12481 cctgatctgt atttttcata cggtcgcctt cttttttcgc tccgttttca tcagtcagat
    12541 catccgccgc attgtcaaca ccgtctgtga catcgtctgt cacatcgtca attccgtctg
    12601 tgacatcatc tgttacgtca tcaactccct gagtcacatc atttaccgca tcatcaagaa
    12661 taccgtctcc ttttttatct gacttgtccg acgcatcgtt gcgatcgttg tctctgtctt
    12721 tatccgttgt cgtctgatcc gccgcattat ctgttttatc tttgcccgca catgctgtca
    12781 tgccaaacaa agtgaacaca cagagcgtca gcaaaatgag ttgtttcatt tttttcataa
    12841 gaatatctcc ctttccataa atattgttgt agttctaata tctgcaatat tttcaatatt
    12901 tatacattcg ggattttttc tttttgctac agagcctttt tcctcagttt ttccatcgct
    12961 tttgattcaa tctgacgcac acgctctctt gttataccaa gactttcacc gatttcttct
    13021 aatgtatatg tgcggtcttt ccccaatcca aaccgaaggc tgatcacttc tttctctctg
    13081 tcgttcaaag tttcaagaag ttcttgtact tcttctttcc tgacaagaac gtccatcgca
    13141 tcttccggcg tcgtctcaga tctgtcttct acaaaatctc caagactgtc ctcgccatct
    13201 tcccctaccg gagtttccaa agataccgga tttcgaatat aagaaagaat atttttcact
    13261 tcctcttctg tccggtcgcc aagcttcttt gcaatctccc ccggttctgc ttctcttccg
    13321 aattcctgct gcagttcttt tgctgctttt tgcacccgtt ttatattttc cgcaacgtga
    13381 accggaactc gaatttctct tgactgctga tcgatggctc ttgtaattgc ctctttgatc
    13441 caccatgacg cgtaagttga aaaacggtta tctttcgtat agtcatactt ttcggcagca
    13501 tgcatcaatc cgatatttcc ttcctgaatc agatccatta acggcaaacc tcttcccgta
    13561 taatgttttg cgatagaaac gacgagacga agattccctt cttccagtct cgctcttgcg
    13621 gattcgtctc cgtctgagat tcttctgccc aattcttttt cttccgtctc gtccaacaga
    13681 ggaatctccc ctatctcatt caaatagatc cgaagcggtt ctgactcctg aatttcttct
    13741 tttcccatac aaacctcact tttatttatc tgttttatat tctctttatg ttctgttata
    13801 tatccgatac ctgctatcgt ttttctctga gctcaccgtt tctgtatgcg acaagcagtc
    13861 gttcaagatc ttttacctga cgtttgagtt cctcaccgat atccgtatct ccgactaaaa
    13921 gcctctccct gactcttttc actatcatcg tttcggaatg gatatctttt tcctttgcga
    13981 tcgccgactc tgtagattca aacggctcgt gcgcaacaag cagaagtcca tatgaattaa
    14041 agatcaaagt ataacctgca attccggttt ctttctgata cgctttggaa aatccgccgt
    14101 cgatcacaag cactttcccg ccgcatttca cgggattttc cccgtctttt cgtttcaccg
    14161 gaacatgtcc gttgacaata tgagacaccg ccgggtc
//
LOCUS       DBMDXF010000026        14172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1254_length_14172_cov_1.921114, whole genome shotgun sequence.
ACCESSION   DBMDXF010000026 DBMDXF010000000
VERSION     DBMDXF010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 14172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14172
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_14172_cov_1.921114"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(117..353)
                     /locus_tag="ACZPD4_02025"
     CDS             complement(117..353)
                     /locus_tag="ACZPD4_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorion class high-cysteine HCB protein 13"
                     /protein_id="HIR1377890.1"
                     /translation="MSDLTATNCGCGCENGNGMSSCLWIILLLACCGGCGNMNSGCGN
                     GCGNDSCLWIILLLLFCGGNSFGGGCGSNCGCGC"
     gene            471..1400
                     /locus_tag="ACZPD4_02030"
     CDS             471..1400
                     /locus_tag="ACZPD4_02030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1377891.1"
                     /translation="MKWVKQSVGYWCDDIKQGRCIGQGVCVALLDTGIARHPDLKGSV
                     VKFCDFTKEGSLPGEKCYDDSGHGTHVAGILCGSGKVSAGAYAGMAPKAKLVVGKVLD
                     KDGNGDVSHVMKGIEWILKERKKYGIRIVNISVGTNPGLAKKQKELLLDAVELLWDTG
                     LTVVVSAGNYGPEEGTVAVPGNSRKVITVGVPDSNLPYSRKSRKSLNYSGRGPTGECV
                     VKPDVFAPGTGVVSCNSMYGMSGEHPYIMKTGTSMAAPVVSGAAACLLSKYPDMTNVE
                     IKLKLRESCVKMKGTESGWGMLHVGRLLGCDKV"
     gene            1457..1807
                     /gene="ylxM"
                     /locus_tag="ACZPD4_02035"
     CDS             1457..1807
                     /gene="ylxM"
                     /locus_tag="ACZPD4_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR1377892.1"
                     /translation="MDKILEQALLYDFYGELLTAHQKEIYEQFVLDDLSLGEIAADAG
                     ISRQGVHDIVKRCARSLEEYERKLHLVEKFLAVKEKVSCIDEILQEWEEEKENPEEII
                     ERLRKLSATIIEEL"
     gene            1882..3243
                     /gene="ffh"
                     /locus_tag="ACZPD4_02040"
     CDS             1882..3243
                     /gene="ffh"
                     /locus_tag="ACZPD4_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1377893.1"
                     /translation="MAFDSLTEKLQNVFRNLRSKGRLTEDDVKAALREVKMALLEADV
                     NFKVVKKFVKDVQERAVGQDVMNGLNPGQMVIKIVNEELVKLMGSETTEIKLQPGSAI
                     TVIMMAGLQGAGKTTTTAKLAGKYKLKGKKPLLVACDVYRPAAIKQLQINGEKQGVEV
                     FSMGDKNRPADIAKAALAHAQKNGNNIVILDTAGRLHIDEDMMAELQQIKEAVEVHQT
                     ILVVDAMTGQDAVNVAESFHNKIGIDGVIVTKLDGDTRGGAALSIKAVTGRPILYVGM
                     GEKLSDLEQFYPDRMASRILGMGDVLTLIEKAGAELDEEKAMQMADKMKKAQFDFEDY
                     LMSMEQMRKMGGLSSIMSMMPGLGGLGGKNKLPDLDSPENDKKMARMEAIIYSMTPEE
                     RKNPDLLNPSRKHRIAKGAGVDISEVNRMVKQFNESRKMMKKLPGMMGGKGGKKGRFR
                     LPF"
     gene            3290..3535
                     /gene="rpsP"
                     /locus_tag="ACZPD4_02045"
     CDS             3290..3535
                     /gene="rpsP"
                     /locus_tag="ACZPD4_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1377894.1"
                     /translation="MAVKIRLRRMGQKKAPFYRIVVADSRSPRDGRFIEEIGTYDPTK
                     DPSEFKVDEELAKKWLNNGAQPTEVVGKIFKAAGIEK"
     gene            3559..3786
                     /locus_tag="ACZPD4_02050"
     CDS             3559..3786
                     /locus_tag="ACZPD4_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1377895.1"
                     /translation="MKELVEVIAKALVDHPEEVSVNEKNEGRTIVLELHVAEGDMGKV
                     IGKQGRIAKAIRSVVKAAAAKEDKKVVVDIM"
     gene            3921..4427
                     /gene="rimM"
                     /locus_tag="ACZPD4_02055"
     CDS             3921..4427
                     /gene="rimM"
                     /locus_tag="ACZPD4_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845382.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1377896.1"
                     /translation="MEQFLQVGAITSTHGIRGEVKVFPTTDDPARFKKLKKVILDTGK
                     RQIDMEIQSVRFFKQFVIVKFKGIDNINDVEQYKGSSLFVSRENAVSLEENEYYIADL
                     IGMEVFTEEGHFGIVKDVMETGANEVYIIDSDVHGEVLVPAIKQCIENVDVENGKMQI
                     RLLEGLIG"
     gene            4455..5339
                     /gene="trmD"
                     /locus_tag="ACZPD4_02060"
     CDS             4455..5339
                     /gene="trmD"
                     /locus_tag="ACZPD4_02060"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1377897.1"
                     /translation="MNFHILTLFPEMVENGLKTSIIGRAVAGGLLSIEAVNIRDFAFN
                     KHQSVDDYPYGGGAGMLMQAEPVYLAYKDIEERIQKRIQNAKMQNAETEEQDAEVKVQ
                     NAGIQDAETVSPDKKLRVVYLSPQGKTFDQKMAEELAEEEDLVLLCGHYEGIDERVLE
                     EIVTDYVSIGDYVLTGGELPAMVMVDTISRLVPGVLHNDVSAEFESFQDNLLEYPQYS
                     RPEEWHGKKVPPVLLSGHHANIEKWRREQSILRTYERRPDLLEKSSLTWKEKKWLEET
                     VKEKTVHEEPEDVTECTN"
     gene            5485..6852
                     /locus_tag="ACZPD4_02065"
     CDS             5485..6852
                     /locus_tag="ACZPD4_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HIR1377898.1"
                     /translation="MKKNIFKKAGRVFTSLALAVSICLTMSVSVFAKNGNDLENKNIN
                     YRDYLYFVIDGKDYKMTDLTTEPGVDLSGSNVSAEVYAKVDMDGLNAAMKASGSKDTF
                     VLHKNTYMSRYEKSTEPIVYNFQDAGNGTYAIVDKSGAHMTLDNLAHVATVGLYIGKT
                     ADSSSYYFGMDAVVTNLFKLNILGKVTAKATVNYKNAEGTILKQLEYETETTGYWGEN
                     VLVEYANLPLIGTSYNLAGDTTGAWISYGYENEINVTVERIEPVFTGLETKVAEVEEF
                     LNKTEDKYTEESMTALTNALEAAKNILANKDNAIQADVDDALTVLTDAKDKLVLVQEE
                     TVNLKALQDKVAEVEKFLAETKTEYTKESMKALKDALADAKAILADPKNAKQADVDNA
                     LKTLTKAKEGLVPVTSDSKGTEAAKQEAKAPVTGDHSFAGITLLASMLCLAAGTIVFV
                     KRKHI"
     gene            6968..7315
                     /gene="rplS"
                     /locus_tag="ACZPD4_02070"
     CDS             6968..7315
                     /gene="rplS"
                     /locus_tag="ACZPD4_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR1377899.1"
                     /translation="MNDIIKKIEAEQLKENAPEFNVGDTVKVYAKIKEGNRERIQVFE
                     GTVLKRQGGGARETFTVRKTSNGIGVEKTWPIHSPHVEKVEVIRRGKVRRAKLNYLRK
                     RVGKAAKVKELVK"
     gene            7495..8169
                     /gene="lepB"
                     /locus_tag="ACZPD4_02075"
     CDS             7495..8169
                     /gene="lepB"
                     /locus_tag="ACZPD4_02075"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1377900.1"
                     /translation="MGELDFRKKRKNTNIRGTARKQGKNSRQRELRFENEKREKRSSG
                     KRWGKNKKNTKGIVLWAVELLLVCMTAVFLVAAFGQRVNVIGDSMSPVLKNGNVVMIN
                     HFIYNIKDPSRGDIAAFQKDGDERYFVKRIVGLPGETVQIKEGKLLIDGKPLKAEYVS
                     DIGYAGTASEPVHLGKDEYFLLGDNETASDDSREEKIGNIKKKEIYGEVWFVIKPWSD
                     IGFVSD"
     gene            8187..9029
                     /gene="ylqF"
                     /locus_tag="ACZPD4_02080"
     CDS             8187..9029
                     /gene="ylqF"
                     /locus_tag="ACZPD4_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR1377901.1"
                     /translation="MHFQWYPGHMTKARRMMQENIKLIDLIIELVDARVPLSSRNPDI
                     DELGKNKARLILLNKSDLAEERINDEWIDYFKDRGYSAVKVNSKKGGGMNQIRSAIQE
                     ACKEKTERDRKRGILNRPVRAMVVGIPNVGKSTFINSLAGKACAKTGNKPGVTKGKQW
                     IRLNKNVELLDTPGILWPKFEDQEVGKKLAFIGSIKDEILQTEELSTELIKFLNAEYA
                     GVLEEKYSIEHLEDVYECLTEIAKSRHCIVRGSELDTEKAAAILLDDFRSGKLGRISL
                     ERPQ"
     gene            9178..9714
                     /gene="lepB"
                     /locus_tag="ACZPD4_02085"
     CDS             9178..9714
                     /gene="lepB"
                     /locus_tag="ACZPD4_02085"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1377902.1"
                     /translation="MKDVIKELVGWIVSILLIVAVSYLIVTFVGQRTQVSGSSMETTL
                     SDGDHLIVDKISYRFREPQRYEIVVFPYRYEKNTYYIKRIIGLPGETVQIVDGYIYIN
                     GKQLDEHYGNEIIEEAGMAAEPVTLGEDEYFVMGDNRNNSQDSRVSDVGAIHRDELMG
                     RAWIRIWPLDQFGVIKHE"
     gene            9707..10474
                     /locus_tag="ACZPD4_02090"
     CDS             9707..10474
                     /locus_tag="ACZPD4_02090"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1377903.1"
                     /translation="MSKSIAQIKEEFGKADEKEREALYSMYDADARSGVQKLIASYRK
                     KEEALRLERERLSLMKSYERKYKDAEMICGIDEAGRGPLAGPVVAGAVILPKDCEILY
                     LNDSKKLSASRREALYDEIMEKAVAVGVGMASPARIDEINILQATYEAMRKAVEQLSV
                     VPDLLLNDAVTIPEMPYRQVPIVKGDAKSVSIAAASVIAKVTRDRLMMQYDKILPEYG
                     FAQNKGYGSQSHIEALKRVGASPIHRRSFIKNFISEE"
     gene            10532..10894
                     /locus_tag="ACZPD4_02095"
     CDS             10532..10894
                     /locus_tag="ACZPD4_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR1377904.1"
                     /translation="MKRNVKQNNRSVGAVYEQAAGYYLEQNGYELIEYNYRCRDGEID
                     IIAKDGDCYVFCEVKYRSGRQAGNPLEAVDQRKQKKIFRCALYYTVQHGIEDAQCRFD
                     VIGVEGTEITHIKNAFVG"
     gene            10899..11746
                     /gene="pgeF"
                     /locus_tag="ACZPD4_02100"
                     /pseudo
     CDS             10899..11746
                     /gene="pgeF"
                     /locus_tag="ACZPD4_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606696.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
     gene            11839..12858
                     /locus_tag="ACZPD4_02105"
     CDS             11839..12858
                     /locus_tag="ACZPD4_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1377905.1"
                     /translation="MQGYLISVLGKAQMFFGRHFLMAAAGSKNVSQSDANNVLETAGE
                     VTAEATEEVNRFIEFFHDNIPNLISFGVKVVLALIFFFIGSKVIKWIRKVIRKSFERS
                     NVDAGVKQFVDSMIKFSLYVILIFMIATNFGVESSSVAALIASAGVAIGLAVQGSLSN
                     FAGGILILVLKPFTVGDYIIVTQENIEGTVKEIQIFYTKLATIDNQTVVVPNSILTNN
                     SLTNVTARPERKLDLKVGISYEADLRKAKQIVEEKLRDDPSVIQDEEKRVFVDSLGDS
                     AVVIGLRAWVKTDEYWTTRWRILEEIKLTFDEEGIEIPYNQLTVHMKTDEETTKGNGN
                     TLGEN"
     gene            12968..13040
                     /locus_tag="ACZPD4_02110"
     tRNA            12968..13040
                     /locus_tag="ACZPD4_02110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13001..13003,aa:Thr,seq:cgt)
     gene            13245..>14172
                     /locus_tag="ACZPD4_02115"
     CDS             13245..>14172
                     /locus_tag="ACZPD4_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR1377906.1"
                     /translation="MERKNIWNLYSDEQILELKEINDKYKRCLDAGKTERECVELSVN
                     MARDAGYRDLEEILRSGETLKAGDKVYAVNMNKMLALFRIGERPISEGMNILGAHIDS
                     PRIDVKQNPLYENEELAYLDTHYYGGIKKYQWVAQPLALHGVIVKKNGETVKVSIGED
                     ENDPVVYITDLLIHLAGKQLQKKAAEVIEGENLDILIGSRPLKDLDDDKKKEAVKENI
                     LRILKEKYDVEEEDFLSAELEVVPAGKARDCGLDRSMVTGYGQDDRVCAYTSFLAMLE
                     MKDPKRTACCLLTDKEEIGSVGATGMQSRFFEN"
BASE COUNT         4722 a         2302 c         3793 g         3355 t
ORIGIN      
        1 caaaaaaggg acggcaaata aagatatgaa aaaggattaa ataaaatata aacagaaaca
       61 ggaaaagatg cgcggaagag accgcgcatc tttttgagac agaggtggaa ttcagattag
      121 catccgcatc cgcagttgga tccgcatccg ccgccgaagc tgttgccgcc gcagaagaga
      181 agaagaagga tgatccaaag acaactgtca ttgccgcatc cgtttccgca tccgctgttc
      241 atattgccgc atccgccgca gcaggcgaga agaaggatga tccaaaggca agaagacatt
      301 ccgtttccgt tttcgcaacc gcatccgcag ttagtagctg ttaaatcact catgttaaat
      361 cctccgaatt gattattgtt tacagtttta tattatgccg gcagatgaaa agtgttcctt
      421 aggggcaaaa gtttcgtgtc gggaatattc tgaataaaaa aggatttctt atgaaatggg
      481 taaaacaatc agtcggatac tggtgtgatg acataaaaca gggaagatgc atagggcagg
      541 gagtgtgcgt ggcgctgttg gataccggga tagcaagaca tcctgatctg aaaggcagcg
      601 tagtgaagtt ttgcgatttt acaaaagaag gtagcctgcc gggagagaaa tgttatgacg
      661 acagcggaca tggaacgcac gtggctggga ttttgtgcgg aagcggaaaa gtgtcagcgg
      721 gagcatacgc aggcatggcg ccgaaagcaa agcttgttgt cggaaaagta cttgataaag
      781 acggaaacgg ggatgtttct catgtgatga aagggattga gtggatttta aaggaaagaa
      841 agaagtatgg gatccgaatt gtgaatattt ctgtcggtac gaatcccgga cttgcaaaaa
      901 aacaaaaaga actgctgctt gatgcagtgg aactgctctg ggataccggt cttaccgttg
      961 ttgtctctgc cggaaactac ggaccggagg agggaacggt ggcagttccg ggaaacagca
     1021 gaaaagtgat tacggtaggc gtgcctgaca gcaatcttcc gtattcgaga aaaagcagga
     1081 agagtctgaa ttattcggga agaggaccga cgggagaatg cgttgtaaaa ccggatgttt
     1141 ttgcacctgg aacaggagtt gtaagctgta acagtatgta tggaatgtcg ggagaacatc
     1201 cttatataat gaagacggga acgtctatgg cggctccggt tgtctcggga gccgctgcct
     1261 gtctgttgtc aaagtaccct gatatgacaa atgtggaaat aaaattaaag ttaagagagt
     1321 catgtgtgaa aatgaaaggg acagaatcag gatggggaat gcttcatgta gggagattgt
     1381 taggctgtga taaagtgtaa agaaaaaaca cttgacacct gaatctgttt cgtgtatgat
     1441 ggcataggtg acaagaatgg ataagatttt agagcaggca ttattatatg atttttacgg
     1501 tgaacttttg accgcgcatc agaaagaaat ttacgaacag tttgtattag acgatctttc
     1561 gcttggggag atcgcggcag atgcgggcat cagccgtcag ggcgtccacg atattgtgaa
     1621 gcgctgtgca agatcactgg aagaatatga acgaaaactt catcttgtag aaaaatttct
     1681 tgctgtaaag gagaaggttt cctgcatcga tgagatattg caagaatggg aagaagagaa
     1741 agaaaacccc gaagaaatta tagagcgtct ccgtaaattg tcggcaacga tcatcgaaga
     1801 attgtaaata tagttgttta tgatggcaag aaaaaagatg atcatggcag atgcaaaagt
     1861 aaaatgtaga ggagtcagac aatggcattt gacagcttaa cagagaaatt acaaaatgta
     1921 ttcagaaacc ttcgcagtaa aggccgtctt acagaagacg atgtgaaggc tgcgcttagg
     1981 gaagttaaga tggcgcttct tgaggcggat gtcaatttta aagtcgtaaa aaaattcgta
     2041 aaagatgttc aggaaagagc agtcggtcag gatgttatga acggactgaa tccgggacag
     2101 atggttatca agatcgtaaa tgaagaactt gtaaagctga tgggatctga gactacagag
     2161 atcaaacttc agccgggaag cgcgatcaca gtgatcatga tggcaggtct tcagggagca
     2221 ggtaagacga caacgacggc aaaactggca ggaaaataca aattaaaagg aaagaaacct
     2281 cttcttgttg cgtgtgatgt ttaccgtccg gcagcgatca agcagcttca gatcaacggt
     2341 gaaaagcagg gcgtggaagt tttttcaatg ggagataaaa accgtcctgc ggatattgcg
     2401 aaagcggctc ttgctcacgc acagaaaaac ggaaataata tcgtgatcct tgacacggcg
     2461 ggacgacttc atattgatga agatatgatg gcagaacttc agcagatcaa ggaggcagta
     2521 gaagttcatc agacgatttt ggtcgtagat gcaatgacag gtcaggatgc ggttaatgtg
     2581 gctgaatcct ttcataataa aataggaata gacggtgtga tcgtcacaaa attagacggt
     2641 gatacaagag gcggtgcggc gctgtcgatc aaggcggtaa caggaagacc gattctctat
     2701 gtcggtatgg gtgagaagct ttctgatctg gaacagtttt atccggacag gatggcatcc
     2761 cgtattctcg gaatgggaga cgtactgaca ctgatcgaga aagccggagc ggagcttgac
     2821 gaagagaaag cgatgcagat ggcagataag atgaaaaaag ctcagttcga ctttgaagat
     2881 tatctgatga gtatggaaca gatgcgtaag atgggcggtc tgtcaagcat tatgagcatg
     2941 atgcccggat taggcggatt aggcgggaaa aataaactgc cggatcttga ttctccggaa
     3001 aatgacaaga agatggctcg catggaagcg atcatatatt cgatgactcc ggaggaaaga
     3061 aaaaatccgg atctgttaaa tccttccaga aaacatcgta tcgcaaaagg tgcgggagtg
     3121 gatatttccg aagtgaaccg catggtaaaa cagttcaacg agtcgcggaa gatgatgaaa
     3181 aaacttccgg gtatgatggg cggtaaaggt ggtaagaagg gcagattcag acttcccttt
     3241 taacacatag ataacaaaac aaattaaaag aaaaacagag gtggaaaaaa tggcagtaaa
     3301 gatcagatta agaagaatgg gacagaagaa agctcctttt tatagaatcg tggtagctga
     3361 ttcaagatcc ccgagagacg gaagatttat cgaggagatc ggaacatatg atccgacaaa
     3421 agatccgagc gagtttaaag tagatgaaga acttgctaaa aaatggctta acaacggagc
     3481 acagccgaca gaagtagtag gaaagatctt caaagcggca ggtattgaga aataagatgt
     3541 ccgcggaggt gcgtacagat gaaagaatta gttgaagtca tagcaaaagc gcttgtagat
     3601 catccggaag aagtttctgt taatgaaaaa aatgaaggac gtacgatagt gcttgagctt
     3661 catgtcgcag aaggcgatat gggaaaagtg atcggcaaac aaggccgtat cgcaaaagca
     3721 atccgttctg ttgtgaaagc ggcagcggca aaagaagaca aaaaagtagt tgtcgatatt
     3781 atgtaatata taataagaaa gtaaggggga cagcgttcgg taagaacagc ctgccccttt
     3841 tcttatgcaa cagtctgatt ttgcagaatc gatacgatgg cagaaagaga gaaaattgat
     3901 tggaacaagg gagaagtgga atggaacagt ttttgcaggt aggagcgatt acttccacgc
     3961 acggaattcg gggagaagta aaagtatttc cgacgacaga cgatccggcg cggtttaaaa
     4021 agctgaaaaa agtgattttg gatacaggaa aacgtcagat cgatatggag attcaatcgg
     4081 ttcgtttttt taaacagttt gtgattgtta aatttaaagg aatcgataat attaacgatg
     4141 tagaacaata taaaggaagt tccctttttg tttcaaggga aaacgcggta agtctggaag
     4201 aaaatgaata ttatattgcc gatctgatcg gcatggaagt gtttacagag gaaggacatt
     4261 tcggaattgt aaaagatgtg atggagaccg gtgcgaacga agtatatatt atagattcgg
     4321 acgttcatgg ggaagtgctc gttccggcga tcaagcaatg tattgaaaat gtagacgtag
     4381 aaaacggaaa aatgcagatc cgtctgttag agggtctgat cggatagatt ttaatgttag
     4441 gagaagcaaa aagaatgaac tttcatatac ttacgttgtt tcccgaaatg gttgaaaacg
     4501 gactgaaaac aagtattatc gggagagcgg tcgccggggg actgctttcg atcgaggctg
     4561 tcaatatacg ggattttgca tttaacaagc atcaaagtgt agatgattat ccgtatggag
     4621 gaggagcggg aatgctgatg caggctgagc ctgtgtattt ggcatataaa gatatcgagg
     4681 aacggatcca aaagcggata cagaatgcaa aaatgcagaa tgcagaaacg gaagaacaag
     4741 atgcggaagt gaaagtgcag aatgcaggga tacaagatgc agaaactgta agtccggata
     4801 aaaaactccg tgttgtctat ctttctcctc aggggaagac ttttgaccag aaaatggcag
     4861 aagaactggc agaggaagaa gatcttgttt tgctgtgcgg gcattatgaa gggattgatg
     4921 aacgtgtttt ggaggagatc gtgacggatt acgtctctat aggagactat gtgctcacag
     4981 gaggggaact tccggctatg gtcatggtag atacgatctc aaggcttgtg ccgggcgtac
     5041 tgcacaatga cgtctcggca gagtttgaat catttcagga taatctgctg gaatatcctc
     5101 agtattccag accggaagag tggcacggga aaaaagttcc gccggtgctt ttgtcgggac
     5161 atcatgcaaa catagaaaaa tggagaagag aacagtcgat tctgcggact tacgagagaa
     5221 gaccggatct tcttgaaaaa agctcgttaa cgtggaaaga aaaaaagtgg ctggaagaga
     5281 cggttaagga aaaaacagtt cacgaagagc cggaagatgt gacagagtgc acgaattgac
     5341 attattacac tttttagctt gaatttcccg aaaacttgaa agtataagaa taaaaggagt
     5401 agaatatttc gttggagagg ttgccggata tgggaatctc tttatcaaat atatctttta
     5461 tataataata gggaggaaga gaaaatgaag aaaaacattt tcaaaaaagc tggcagagtg
     5521 tttacatcac tggctcttgc agtgtctatt tgtctgacga tgtcggtttc tgttttcgct
     5581 aaaaacggta acgacttgga aaacaaaaac atcaattatc gcgattatct ttattttgtg
     5641 atcgatggaa aagattacaa gatgacagat cttacaacag agccgggcgt agatctttcg
     5701 ggaagcaatg tgtctgccga agtttacgca aaagttgata tggatggttt gaatgcggcg
     5761 atgaaggcgt caggttccaa agatacattt gtcctgcaca agaatacata tatgtcacgt
     5821 tatgaaaaat cgacagagcc gattgtgtat aatttccagg atgccggaaa cggtacatat
     5881 gcgatcgtag ataagtcggg agcacatatg acattggata accttgcgca tgtagctact
     5941 gtgggattgt atatcggaaa aacagcggac agctccagct attatttcgg tatggacgca
     6001 gttgtaacaa atcttttcaa actgaatatt cttggaaaag tgacagcgaa ggcaactgta
     6061 aactataaaa acgcagaagg aacgatttta aaacagctcg agtatgagac ggaaacaaca
     6121 ggatattggg gcgagaatgt tcttgtagaa tatgcgaatc ttccgttgat cggaacatct
     6181 tataatcttg cgggagatac aacaggcgca tggatcagtt acggatatga aaatgagatc
     6241 aacgtaacag ttgaaagaat tgagccggtc tttactggtc tggaaactaa agttgcagaa
     6301 gtagaggagt tccttaacaa aacagaagac aaatacacag aagaatcaat gacggctctt
     6361 acaaacgcac ttgaagcggc aaaaaatatt cttgcaaaca aagacaatgc aatccaggca
     6421 gatgtggatg acgctttgac ggtgttgaca gatgcgaaag acaagcttgt tttggttcag
     6481 gaagaaacag ttaatctgaa ggcgttgcaa gacaaagttg cagaagtaga aaaattcctt
     6541 gcagagacaa agacagaata tacaaaagag tctatgaaag ctttgaaaga cgcgcttgca
     6601 gatgcaaaag caattcttgc agatccgaaa aatgcaaaac aggcggatgt agataacgcg
     6661 ttgaaaactt tgacaaaagc aaaagaaggt cttgtaccgg taacaagcga ctcgaaagga
     6721 acggaagctg caaagcagga agcaaaagct ccggttacag gagatcattc gtttgcggga
     6781 atcacgcttt tggcatctat gctttgtctt gcggcaggaa cgatcgtgtt tgtaaagaga
     6841 aaacacattt aggacttgtc aatcggcaca aaatatgata gactacagaa gttgtgaaaa
     6901 gaagagcgat ggtcctctga cacattttta gtgttaagaa cgtcaaatat gaaggaggtc
     6961 acacaaaatg aacgatatca ttaagaaaat cgaagcagaa cagttgaaag aaaacgcacc
     7021 ggaatttaat gtcggcgata cagtaaaagt atacgcgaag attaaagagg gaaaccgcga
     7081 aagaatccag gtttttgaag gaacagttct gaaaagacag ggcggcggag caagagagac
     7141 tttcacagta agaaagacat ctaacggaat cggagttgag aagacatggc cgatccactc
     7201 gccacatgtt gaaaaagtag aagttatccg cagaggtaaa gtaagaagag cgaaactgaa
     7261 ctacttgaga aaacgtgtag gaaaagctgc aaaagtaaaa gaattagtaa aatagtaacc
     7321 agtgagaggg acaattacag atttgtatat tgtccctttt ttgtaattgc ggcgagcgaa
     7381 agcccgggct tgccgagatc ttggcgtccg cttacgatct taaattttcc ggaagaggga
     7441 aaagaaagat ggcagaggcg ggcagtcggc agaagcgatt agagcggagg ggttatggga
     7501 gagttagact ttcggaagaa acgtaaaaat acaaacatac ggggcacggc aagaaagcag
     7561 ggaaagaatt ccagacaacg cgaactgaga tttgaaaacg aaaaaagaga gaaacggtct
     7621 tccggtaaac gctggggaaa aaataaaaaa aatacaaaag ggatcgtttt gtgggcagtg
     7681 gaacttttgc tcgtatgtat gacggcggtt tttctcgtag cagctttcgg acagagagtg
     7741 aatgttatcg gagattcgat gtcgcctgtt ttaaaaaacg gaaatgttgt gatgataaat
     7801 cactttattt ataatataaa agatccttcg agaggagata tagcggcgtt tcaaaaagac
     7861 ggagacgagc ggtattttgt aaaacggatc gtaggactcc cgggagagac ggtgcagatc
     7921 aaagaaggaa aactgcttat cgacggaaaa ccgctgaaag cagagtatgt gtcggatatc
     7981 ggatatgcgg gaacagcgtc cgaaccggtc catctgggaa aagatgaata tttccttttg
     8041 ggagataatg agactgccag tgatgacagt cgtgaagaaa aaatcggcaa tattaagaaa
     8101 aaagagattt atggtgaagt ttggtttgtt ataaaaccgt ggtcagacat cggttttgtt
     8161 tcagactgat tttaggaggg agattcatgc actttcaatg gtatcccggg catatgacga
     8221 aagcccgcag aatgatgcag gaaaatataa aactgatcga tctgatcata gagcttgtag
     8281 atgcgcgcgt tccgcttagc agccggaatc ctgatattga tgagctcggg aaaaataagg
     8341 cgcgtctgat cctgctgaac aaatcagatc ttgcagagga acggatcaat gacgaatgga
     8401 tcgactactt taaagacaga ggatattctg ccgtaaaggt gaactcaaaa aaaggcggcg
     8461 gtatgaatca gatccgttct gcgattcagg aggcgtgtaa ggaaaaaaca gagcgcgacc
     8521 gtaaaagagg aattttaaat cgccctgtcc gcgcgatggt tgtcggcatt ccgaatgtcg
     8581 ggaagtctac gtttatcaat tccctcgcgg ggaaggcatg tgcgaagact gggaataagc
     8641 cgggcgttac aaaagggaaa cagtggatcc ggcttaataa aaatgtagaa ttacttgaca
     8701 ccccgggaat tctctggccg aagtttgaag atcaggaagt cggaaaaaaa ctggcgttta
     8761 tcggttcaat caaagatgag attcttcaga cagaagaact ttccacagaa ctgatcaagt
     8821 ttttaaatgc agaatatgcg ggagtgctgg aagaaaaata cagtatcgaa catttggaag
     8881 atgtatatga atgtcttacg gagattgcaa aaagcagaca ttgcatcgtg cgcggaagcg
     8941 aacttgacac ggaaaaagcc gcagcgatcc ttcttgatga ttttcgcagc ggaaagctgg
     9001 ggagaatctc attggaacgt ccacagtaat tataaaaaga aattcacaaa gcgggaagag
     9061 aagatcgtga acgtcagata gaaaaaaaga ctgcgggcgg aaaatgtgaa gaaaaagagc
     9121 agaagcagca aaaagaatga ctgcaaaaag atgtgcatat agcagaggtt gacttgaatg
     9181 aaagatgtaa taaaagaact tgtaggatgg atcgtatcga ttctgctgat cgtggcggta
     9241 tcttatctga tcgttacatt tgtcggacag agaacgcagg taagcggttc atctatggag
     9301 acaactttgt cggacggaga tcaccttatc gtagataaga tttcgtaccg gttccgggag
     9361 ccgcagcgtt acgagatcgt tgtatttccg taccgatatg aaaaaaatac ttactatatt
     9421 aaacggatca tcggacttcc gggggaaacg gttcagattg tagacggata tatttatata
     9481 aacggaaaac agcttgatga gcattacgga aatgaaataa tagaagaagc gggaatggcg
     9541 gcagaaccgg ttactctcgg agaagacgag tattttgtga tgggagataa tcgaaataac
     9601 agtcaggaca gcagggtgtc cgatgtgggc gcgatccata gagatgaatt gatggggcgc
     9661 gcgtggatac ggatctggcc gcttgatcag ttcggggtga taaaacatga gtaaaagtat
     9721 tgcacagatc aaagaagaat tcggaaaagc ggatgagaag gaacgagaag cgctgtacag
     9781 tatgtatgat gctgatgccc gcagcggcgt tcagaaactg atcgcttcct accgaaaaaa
     9841 agaagaggcg cttcgcttag agcgggagcg cctttctctc atgaaaagtt atgagagaaa
     9901 atacaaagat gcagaaatga tatgcggaat cgatgaagcg ggaagaggac cgctggcagg
     9961 gccggttgtg gcaggcgcgg ttattttgcc gaaagattgt gaaatcttat atctgaacga
    10021 ttctaagaag ctttcggcat cgagaagaga agcgctctac gatgaaatta tggagaaagc
    10081 agttgctgtc ggcgtcggaa tggcaagtcc cgcaaggata gacgagatca atattctgca
    10141 ggctacatat gaggcgatgc gtaaagcggt agagcagctt tcggttgtac cggatcttct
    10201 tttaaacgat gcggttacga ttccggagat gccctatcgt caagtgccga tcgtgaaagg
    10261 agatgcaaaa agtgtttcca ttgcggcggc aagcgttatc gcgaaagtga caagagacag
    10321 actgatgatg cagtatgata agatccttcc ggagtatgga tttgcacaaa ataaagggta
    10381 cgggtcgcaa agtcatattg aagcgttgaa acgtgtcgga gcaagtccga tccacagacg
    10441 ttcgtttatc aaaaatttta tttcagaaga ataaagacag aaaaaacatt taaaacagaa
    10501 tttcaaaaat tatgaaaaag aatgtggtaa aatgaagcga aacgtaaagc aaaacaacag
    10561 atctgtcgga gctgtatacg agcaggcggc aggatattat ctggaacaaa acggttatga
    10621 actgatcgag tataattaca gatgccgtga cggcgagatt gatattattg caaaagacgg
    10681 agactgttat gtgttttgcg aagtgaaata tcgttccggc agacaggcgg gcaatccgct
    10741 ggaggcggtc gatcaaagaa agcaaaaaaa gatttttcgg tgtgcgctgt actatacggt
    10801 acagcatgga attgaagatg cgcagtgccg ctttgatgtg atcggggtgg aaggtacaga
    10861 gattacgcat ataaagaatg catttgtggg gtgacggtat gagtttaggg ttagtttata
    10921 aaaatggaga acatattttt gacgaagtgt gcggagaagt tccgtttttg acatatccgc
    10981 tatttgaaaa gacgggagaa aaaaaagcgc tttttcgaca aggatcggcg gagtgagcaa
    11041 aggctgttat gagtcgatga accttagttt tgacagaggg gatgacaaag atgccgtcat
    11101 ggaaaatttt cagaggatcg catctgcaat cggaacaaac tgtcacgata tggtcttgtc
    11161 acgccaaaca catacggcaa atgtgcgtgt tgtcacggaa gaagataaag gaaaagggat
    11221 tgttcgcgaa cgggactata cagacgtaga tggtcttgtg acaaacgtgc cggggatttg
    11281 tcttgtcact tcttatgcag attgtgttcc gctttatttt gtagatccga taaaaaaagt
    11341 catcggtctt agtcattcgg gatggagggg aactgtcgga aagataggaa aagagacggt
    11401 tcgtgttatg aaagaacatt tcgggtgtga tccggcggat atattggcgg cagtcggacc
    11461 ttcgatctgt caggattgct atgaagtaag cggcgatgtg attgaaaaat tcaaagaagc
    11521 tttcgatgaa aaagattggg ataatctgtt ttacagaaaa aacaatggaa aatatcagct
    11581 tgatctatgg aaagcaaacg aatcggtttt tcgggaatcg ggaatctgca aggaacatat
    11641 tgcggtcacg aatgtctgta cgcattgcaa tagcaagatc ttatattcac atcgggagac
    11701 aggtgataag cggggaaatt tgtgcgcgtt tcttgcatta aaataaaatt tcttttagaa
    11761 aaacacggaa caggaaaaac agagaaaagc agatcttatc gaattgtaaa aaataaaaga
    11821 tgaaaggaaa aatatagaat gcaggggtat cttatcagtg tgcttggcaa agcgcaaatg
    11881 tttttcggca gacactttct tatggctgcg gcaggcagca aaaatgtgtc gcagtcagat
    11941 gcgaataatg ttcttgaaac agccggagaa gtgacggctg aggcaacgga agaagtgaac
    12001 cggtttatcg agttttttca tgacaatata ccgaatctga tcagcttcgg agtgaaagtt
    12061 gttctggcgc ttatcttttt ctttatcggg agtaaagtga tcaagtggat tagaaaggtg
    12121 atcagaaaat cgtttgagag aagcaatgtt gatgcaggtg tgaaacagtt tgtcgattct
    12181 atgatcaaat ttagtttgta cgttatattg atcttcatga tcgcaacaaa cttcggggta
    12241 gagtcatcgt ctgtagcggc attgatcgct tcggcaggtg ttgcgatcgg tctggcggtt
    12301 cagggaagtc tgtctaattt tgcgggaggt attttaatac ttgttttaaa accttttacc
    12361 gtaggcgatt atattatcgt gacacaggaa aatatcgaag gaactgtaaa agagatacag
    12421 atcttttata caaagctggc gacgatcgac aaccagacgg tagttgttcc gaacagtata
    12481 ctgacaaata acagtcttac aaatgtaacc gcaagaccgg agcggaaact ggatttgaaa
    12541 gtcggcattt cttacgaagc ggatcttcgc aaagcgaaac agatcgtaga agaaaaactg
    12601 cgtgacgatc cgtctgtcat tcaggatgag gagaaaagag tatttgtaga ttcgcttgga
    12661 gacagcgctg tcgtgatcgg acttcgggcg tgggtcaaga cggatgagta ttggacgaca
    12721 agatggcgta tacttgagga gatcaagctg acttttgatg aagaaggtat agagattccg
    12781 tataatcagc tgacagttca tatgaaaacg gatgaagaaa ctacaaaagg aaatggaaat
    12841 accctcgggg aaaattagcg gcgggaaaat ccacggggaa aacgtttgga aaaaactaaa
    12901 aaagtagttg aaatttacat taaagtaaga tataataatt aaggttcgta ttagtacgaa
    12961 tcttaatgcc ggaatagctc agcaggtaga gcacttcact cgtaatgaag gggtcgtcag
    13021 ttcaagtctg atttccggct taataaaaac gcagttccgt tttttcggaa ctgcgttttt
    13081 ttatggcgct cgccgcgttc cggaaagtgt ctttcggcgc tttatgtggc tgcgttttcc
    13141 ggttgaaaat taaaaagttt gtttactttt aaaatacaga gaaatcgtat ataataaagt
    13201 gaaaataaaa aagatcataa aataataaag aaggggactg ccttatggaa agaaaaaata
    13261 tctggaattt atacagcgat gaacagattt tggaactaaa ggaaatcaat gacaagtaca
    13321 agcgttgcct ggatgccgga aagacagagc gggagtgcgt agaacttagc gtaaatatgg
    13381 caagagatgc aggttaccgg gatcttgaag aaatattaag atcaggagaa acgctgaaag
    13441 cgggagataa agtatacgca gtcaatatga ataagatgct tgctttattt agaatcggtg
    13501 agagaccgat ttctgaggga atgaatattc ttggagcaca tattgattcg ccgcgtattg
    13561 acgtgaaaca aaatcctctc tatgaaaatg aagaacttgc ttatcttgat actcattatt
    13621 atggcggtat aaaaaaatat caatgggtgg cacagccgct cgcactgcac ggcgtcatcg
    13681 tcaaaaagaa cggagaaact gtgaaggtat ccatcggaga agatgaaaat gatcctgttg
    13741 tttatattac agatcttctg attcatctgg caggaaaaca gcttcagaaa aaagcagctg
    13801 aggtcattga gggtgaaaat cttgatattc tcattggaag cagaccgctg aaagatcttg
    13861 atgacgacaa aaagaaagag gctgtaaaag aaaatatcct cagaatcctg aaagaaaaat
    13921 atgatgtaga ggaagaggac ttcctttctg cagagcttga ggtagttccg gcaggaaaag
    13981 caagagactg cggactggac agaagcatgg ttacgggcta tggacaggac gaccgcgtat
    14041 gcgcatacac ttccttcctg gcaatgctgg aaatgaagga tccgaagcgt acagcctgct
    14101 gtcttcttac agataaagaa gagatcggca gcgtaggagc aacaggaatg cagtccagat
    14161 tttttgaaaa ca
//
LOCUS       DBMDXF010000027        14036 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1264_length_14036_cov_2.340007, whole genome shotgun sequence.
ACCESSION   DBMDXF010000027 DBMDXF010000000
VERSION     DBMDXF010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_14036_cov_2.340007"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="ACZPD4_02120"
     CDS             complement(<1..1154)
                     /locus_tag="ACZPD4_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1377907.1"
                     /translation="MTKIDIISGFLGAGKTTLIKKLLSEAFAGEQVVLIENEFGEIGI
                     DGGFLKESGIEIREMNSGCICCSLVGDFGKSLREVVDTYHPDRILIEPSGVGKLSDVI
                     KAVQDVQSEIDAKLNSFTTVVDVTKCRIYRKNFGEFFSNQIEYAGAVILSRTDKAKPE
                     KIEESIAILRELNPKAPFITTPLEQLAGSKILETMESVRSMEEELLAEVVCPECGHHH
                     GAHHHDHGEDEHHHEHHHHDHGECEHHHEHHHHDHGECEHHHEHHHHDHGECEHHHDH
                     HHHDNGEDGPHHDHHHHDCGCGHDHAGHHHADEVFTSWGRETIHVYSEEQVGEILKSL
                     ENEEEYGDILRAKGMVKGEDGTWIYFDMVPGEYEVRTGAPEYTGRICVIGA"
     gene            complement(1179..1931)
                     /locus_tag="ACZPD4_02125"
     CDS             complement(1179..1931)
                     /locus_tag="ACZPD4_02125"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1377908.1"
                     /translation="MKFVSWNVNGIRACVQKGFLDFFTEADADIFCIQESKMQEGQLE
                     LDLPGYHQYWNYAVKKGYSGTAVFTKKEPLSVTYGIGIEEHDQEGRVITCEFEDFYFV
                     TVYTPNSQNELARLDYRMRWEDDFRMYLKKLEEKKPVIVTGDMNVAHKEIDLKNPKTN
                     RKNAGFTDEERGKFTELLDAGFIDTFRYFYPDREGIYSWWSYRFSARAKNAGWRIDYF
                     CVSESLKERLKDAKILTDVMGSDHCPIELDMD"
     gene            complement(2034..2816)
                     /gene="truA"
                     /locus_tag="ACZPD4_02130"
     CDS             complement(2034..2816)
                     /gene="truA"
                     /locus_tag="ACZPD4_02130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1377909.1"
                     /translation="MRTYKLTIAYDGTNYQGWQRQSDTDRTIQGILEETLENCIGYQV
                     QIDGSGRTDAGVHASGQTASVVLPGKVDTALFTEKMNGCLPPDIRILDVELVKNGFHA
                     RKSAVGKVYEYRIDTGIKQDVFTRKYSGHFPGEPDLARMREAAGYLTGTHFFAGYTDK
                     KDNRSVRRTIYSIMIRDERKDFLPCGKSDQIGNGNCIVIRYDGTGFLYHMVRILTGTL
                     LEVGMRERTAESVKEALLTGDRSKAGFLAPAGGLFLREVRYE"
     gene            complement(2825..3274)
                     /gene="nrdR"
                     /locus_tag="ACZPD4_02135"
     CDS             complement(2825..3274)
                     /gene="nrdR"
                     /locus_tag="ACZPD4_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR1377910.1"
                     /translation="MRCPYCGNPDTRVIDSRPAEDKNSIRRRRSCDECGRRFTTYEKV
                     ETIPLIVIKKDNNREQYERSKIEQGVLSACYKRPVPAEAIQKTIDAVELEIFNREEKE
                     IPSSEIGEIVMEKLKELDPVAYVRFASVYREFKDAGTFMDELKKFLQ"
     gene            complement(3333..3587)
                     /locus_tag="ACZPD4_02140"
     CDS             complement(3333..3587)
                     /locus_tag="ACZPD4_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR1377911.1"
                     /translation="MRICELKEKEVINVCSGRRLGCIADVEINICTGEIEAIVIPGPG
                     KICGFFGTDCEYVIPFSCIKKIGPDIVLVEIKEEKFLQKF"
     gene            3756..4130
                     /locus_tag="ACZPD4_02145"
     CDS             3756..4130
                     /locus_tag="ACZPD4_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HIR1377912.1"
                     /translation="MKEMILLFNPPAKEQLLKIRSALLPLHIRIQLIKPEQYNQPLGY
                     LAGMKDISPVEGNYAGEELPDTMFLFSSLSSSRLDQVLAALRKSGAGPFPYKAVLTAT
                     NSKWNALDCFNEIRREHEALHP"
     gene            4198..5772
                     /locus_tag="ACZPD4_02150"
     CDS             4198..5772
                     /locus_tag="ACZPD4_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1377913.1"
                     /translation="MWKQKTLKKSSKSCMKPAYTRMVSVCFIISLLTTAYPVSTTFLN
                     LQKLSGPAYTDAAFSLSITNSEAFAETLGLILGGNAFPKLLQNTVFHVFSFVIDLCSA
                     GISIFFNVLRTVNAFFTEPLGTSFVFLIIGLCITFLYQLFVNYVLIIGEMRFFLEVRK
                     YKRTSISKIFFLYKLRCIAAPVWVMFCRSVFQFLWNFTIAGGIIKHYEYAMIPYILAE
                     NPKISRKDAFFLSKQLMNKNKWRVFLLDCSFLGWKILSILTLGILDFIFVNPYITGCK
                     AELYASLRRDYVLSRSPRYEMLSDSYLEHVPSEDELLISKALYDDSQGPYTKTSYFEP
                     EQYPVFLFSVQPKTVKPSARVFRNYDILSCIFLFHAASCFGWIMEMLIHLMRDGTVAD
                     IHLLFGPWLPLYGIYGIIILKASKRLLKKPIFVFFLNFIVFSFLQYIFSFTVELFSGY
                     KLWDFSEFFLNINGRIYLGGSAAFALLGCAFIYYLAPNWTNYFSKLSKKTQTVFCVIL
                     NSLFITDVILTLIFSY"
     gene            5919..6728
                     /gene="trhA"
                     /locus_tag="ACZPD4_02155"
     CDS             5919..6728
                     /gene="trhA"
                     /locus_tag="ACZPD4_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR1377914.1"
                     /translation="MGNTHAIAKKSTFIKKHPTLHIQNYAGNSYSAAGYKKTGYNSGK
                     SASLNELRGKFYIKEPGSAITHFIGMIMALIAAFPLLIKAAAQPDPVYLISMSVYAAS
                     LILLYAASTTYHTFDISEKGNTVLKKIDHMMISVLIAGSYTPVCLLVLEGRMRIILLA
                     VVWTLALTGILIKAFWVFCPKWVSSVLYIGMGWTCVLAFAQIFRNMPLAAFGWLLAGG
                     IIYTVGGIIYALKVPIFNNKHKNFGSHEIFHLFVMGGSICHFIVMYGYLLP"
     gene            complement(6810..7553)
                     /locus_tag="ACZPD4_02160"
     CDS             complement(6810..7553)
                     /locus_tag="ACZPD4_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1377915.1"
                     /translation="MLTEQRFERILERLKVKKSITVAEITEDLGISESTARRDITALD
                     KAGKLTKVFGGAVAAEHTYEMKEPTVAQKVNVRKEEKIKIAQYAADMIGDNDFVYLDA
                     GTSVGYMVEFLAEKRAAFVTNAVAHAQRLAAQGNRVLLIGGELKSSTEAVVGAQAIEL
                     LRKYHFTKGFFGTNGITKEEGFTTPDVGEAQVKQTAVQQCQKSYILADSTKFGNISAV
                     TFAPFSGSVILTEETVDGYKENERLIVCR"
     gene            complement(7776..9692)
                     /locus_tag="ACZPD4_02165"
     CDS             complement(7776..9692)
                     /locus_tag="ACZPD4_02165"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIR1377916.1"
                     /translation="MRITDLLDKRSISLSETPKTKSEALDRIIDLMVASGKINDREAY
                     REQVYAREEESTTGIGEGIAIPHGKCDAVTKPGLAAMVVKDGVEFDALDGQPVTLMFL
                     IAAPNTKDNVHLDVLSKLSVLMMDEVFADALRNAKSADEFLDIIDKADAEQGDIDERL
                     ADMNEASANGFKILAVTSCPTGIAHTYMAAEGIEKAAKEKDCFVKIETRGSGGAKNVL
                     TEKEIKEADCIIVAADAQVPMDRFDGKKVIICQVSDGISKAPQLIDQAMSGNVPVYHS
                     GNETSSSTAVSSSGKSGGIGHKIYTQLMNGVSHMLPFVVGGGILIAIAFLIDGLSIDL
                     NSLPADERAAFGTITPAAALFKQIGGVAFGFMLPVLAGYIAMAIGDRPALAVGFVGGM
                     IAAGGKSGFLGALVAGFAAGYIIVGLKKVCGKLPEAVEKIAPVLLYPVFGILIMGLLM
                     TFAVEPVMGGINTALNAGLSSMSGTSKILLGMIVGGMMSIDMGGPFNKAAYVFGTASI
                     AAGNYDIMAAVMVGGMTAPCAIALATMLFKNKFTKEQRDAGPTNFIMGLAFITEGAIP
                     FAASDPLHVLPACIAGSAVAGALSMAFNCTLMAPHGGIFVFPVVGNPLQYLIALVAGT
                     VVSALLLGVLKKKA"
     gene            complement(9924..10832)
                     /gene="pfkB"
                     /locus_tag="ACZPD4_02170"
     CDS             complement(9924..10832)
                     /gene="pfkB"
                     /locus_tag="ACZPD4_02170"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR1377917.1"
                     /translation="MIYTVTFNPSLDYIVSVNDFQLGLTNRTDSELILPGGKGINVST
                     ILMNLGIDSTAFGFAAGFTGEEIIREVEAMGIRSDFIKIDSGISRINLKLKNIDGTEI
                     NGSGPEISEEKIEELLRKLDILGEGDILVLAGSIPASMPADMYSTIMERLQHKNVTFI
                     VDATKDLLINVLKYKPFLIKPNNHELGELFDVKLTTREEVIPYGKKLQKQGARNVLIS
                     MAGEGAVLVAEDGSVYEAPAPKGTLVNAVGAGDSMVAGFTAGWIEKKDYRHAFYMGVS
                     AGSASAFSEYLATKEEIMDLYEKVSK"
     gene            complement(11093..11668)
                     /locus_tag="ACZPD4_02175"
     CDS             complement(11093..11668)
                     /locus_tag="ACZPD4_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1377918.1"
                     /translation="MINEAIEFAAKAHEGQLRKGTKKPYIVHPIEVSEIVSAMTDDEE
                     VICAAVLHDTIEDCRGITKDVLKLRFGDRVAELVAQESEDKTLSWKERKSATIARLKD
                     AQREVQLIGLADKLSNMRDIDRDYPVVGEKLWERFRMKSKAALEWYYKGVRDALKENF
                     SEEEEYKEYCGLIEKNFGSSEIDMSWDKQDV"
     gene            11857..12588
                     /gene="ymfI"
                     /locus_tag="ACZPD4_02180"
     CDS             11857..12588
                     /gene="ymfI"
                     /locus_tag="ACZPD4_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P 5-aminopentanone reductase"
                     /protein_id="HIR1377919.1"
                     /translation="MKKKYVLITGSSRGIGRACAVAFAKNGYHVFINCKNSVEQLAET
                     EHEILASGGGCTALPGDVSDPDVVRSIFCKIKKVCQGVDVLVNNAGISHFGLLSDLSD
                     SEWKNLIDTNLSSAFYCCREAIPHMVSEKCGKIINISSMWGTAGASCEAAYSASKSGL
                     HGLTKALAKELAPSGISVNAVACGVIDTDMNSRLNEEEKNMLCEEIPAGRFGTCEEVA
                     RTVLALAESPAYLTAQIIGIDGGFL"
     gene            12718..13587
                     /locus_tag="ACZPD4_02185"
     CDS             12718..13587
                     /locus_tag="ACZPD4_02185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1377920.1"
                     /translation="MPYSIMLDAGHGGRDPGAVYNGRQEKIDTLSLTLAIGQILQERG
                     IDVLYTRTTDIYESPYQKAMEANEAGVDFFISIHRNSFPQDNAVSGVESLVYDKSGIK
                     LEMAENINEQLEGIGFVNLGVKARPGLVVLRRTKMPAVLVEVGFINSDTDNMLFDDNF
                     SDIALAIAEGILDTLGINTPVVPLPDENEAPVAPLPDENTYPDDAFPNEDNVPVVPLP
                     PRYTVQTGAFRNPLYAERLRRELSELDFPASVVEGNGLYRVMVGVFPTLHDAAETERR
                     LRSAGYQTVIVSQ"
     gene            complement(13695..13976)
                     /gene="spoVG"
                     /locus_tag="ACZPD4_02190"
     CDS             complement(13695..13976)
                     /gene="spoVG"
                     /locus_tag="ACZPD4_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR1377921.1"
                     /translation="MQITDVRVRKVAKEGKLKAVVSITIDDEFVVHDIKVIEGEKGMF
                     IAMPSKKAMDGEYRDIAHPINSNTRERIQSMILEKYEQALAEEPDGENP"
BASE COUNT         3556 a         3615 c         2434 g         4431 t
ORIGIN      
        1 gctccgatca cacaaatacg tcccgtgtat tccggcgctc cggtacgcac ttcatattct
       61 cccggaacca tgtcaaagta gatccatgtt ccgtcttctc ctttgaccat tccttttgct
      121 cgaaggatat cgccatactc ttcttcattt tcaagactct ttaaaatctc cccgacctgt
      181 tcttccgaat acacatggat cgtctcacgc ccccagctcg taaacacttc gtctgcatgg
      241 tggtgaccgg catgatcatg accacatcca cagtcatgat gatggtggtc gtggtgaggt
      301 ccgtcctctc cattgtcatg atgatggtgg tcgtggtgat gctcgcactc tccgtggtca
      361 tgatggtgat gctcgtgatg atgctcgcac tctccgtgat catgatggtg atgttcgtga
      421 tgatgctcgc actctccgtg gtcatgatgg tgatgttcgt gatgatgctc atcctctcca
      481 tggtcatgat gatgcgctcc gtggtgatga ccgcactccg ggcagacaac ctccgcaagg
      541 agttcctcct ccattgatct gacggattcc atcgtttcca aaattttact tcccgcaagt
      601 tgttccaaag gtgtagtaat aaacggcgcc ttcggattca attctcttaa aatagcaata
      661 ctttcttcga tcttttccgg ttttgcctta tctgtcctgc tcaaaataac cgcgcccgca
      721 tattctatct gattactgaa aaattcaccg aaattttttc tgtaaattct gcatttcgtc
      781 acatccacaa cagtcgtaaa actatttaac ttagcgtcga tctcactttg tacgtcttga
      841 accgccttta tgacgtctga taattttccg acccccgacg gttctatcaa aatacgatcc
      901 ggatgataag tatccacaac ttctctcaaa gatttgccga aatctccgac aagagaacaa
      961 cagatacaac ccgaattcat ttccctgatc tcgattcctg attcttttaa aaatccgccg
     1021 tcaatcccga tctctccaaa ctcattttcg ataagaacaa cctgttctcc cgcaaacgcc
     1081 tccgaaagca gttttttaat aagcgttgtt tttccagctc ctaaaaagcc cgaaataata
     1141 tcaatctttg tcatttcaaa caacctccca tattatattc aatccatatc cagctcaata
     1201 ggacaatgat ccgatcccat cacatctgtc aaaatcttcg catctttcag acgctctttc
     1261 aaactttccg aaacacagaa ataatcgatc cgccatccgg cattttttgc tcttgcgctg
     1321 aagcgatacg accaccagga ataaattcct tcccgatcgg gataaaaata ccgaaacgta
     1381 tcaataaatc ccgcgtcaag aagttcggta aactttcctc tctcctcatc tgtaaatccg
     1441 gcattttttc gatttgtctt tggattcttc agatcaattt ccttgtgcgc cacattcata
     1501 tctccggtta cgatgaccgg ctttttttct tccagttttt tcaggtacat acgaaaatca
     1561 tcttcccatc tcatgcggta atcaagtctc gccaattcat tttgcgaatt gggcgtatat
     1621 accgtaacaa agtagaaatc ttcaaattcg catgtgatca ctctgccttc ctgatcatgt
     1681 tcttcgattc cgataccgta agttacagac aacggttcct ttttcgtaaa caccgccgtt
     1741 cccgaatatc cttttttcac cgcataattc caatactgat gatatcccgg caaatcgagt
     1801 tccaactgcc cttcttgcat ctttgattcc tgtatgcaga aaatatccgc atctgcctca
     1861 gtgaaaaaat ccaaaaaacc tttttgcaca cacgctctta ttccatttac attccacgaa
     1921 acaaatttca ttatcatcca cctctctcac ataatattaa tatcactctg atagaacctg
     1981 tttccgggta aagcataaaa ggacagatgc tttcactacc tgtccctctc ttctcattca
     2041 taccgtactt ctctcaaaaa aagtccgcct gccggagcca gaaaccccgc ttttgatctg
     2101 tctcctgtca aaagcgcctc tttcacactt tcggcggtac gttcccgcat cccgacttca
     2161 agtaaagttc ctgtcaatat tctcaccata tgatacaaaa atcccgttcc gtcatatcga
     2221 atgacaatac agtttccatt cccgatctga tcggattttc cgcacggcaa aaagtctttt
     2281 ctttcatctc tgatcatgat cgagtaaatc gtacgtctga cagatctgtt atctttttta
     2341 tccgtatacc ccgcaaaaaa atgtgtgcct gtcaaatatc cggccgcctc cctcatcctt
     2401 gcaagatccg gttctcccgg aaaatgaccg ctgtacttcc gtgtaaatac atcttgtttg
     2461 atccccgtat cgatccgata ttcataaact ttccctaccg cacttttccg ggcatgaaat
     2521 ccgttcttca caagctccac atcaaggatg cgtatatcag gcggaagaca cccgttcatt
     2581 ttttccgtaa acaaagccgt atccactttt cccggcaaaa caacgctcgc agtctggccg
     2641 cttgcatgaa ctccggcatc ggttctcccg gagccgtcga tctgcacctg atatcctatg
     2701 cagttttcca gcgtctcttc caaaatcccc tgtatcgtcc gatctgtatc tgactgcctc
     2761 tgccagcctt gatagtttgt cccgtcatag gcaatcgtca atttatatgt tctcataatt
     2821 ttatctactg caaaaacttc ttaagctcgt ccataaatgt gcccgcatct ttaaactctc
     2881 tgtacacaga ggcaaaacgt acatatgcta ccggatcaag ctcttttagt ttttccataa
     2941 cgatttcccc gatctcactg ctcgggattt ctttctcctc acgattaaaa atctcaagtt
     3001 cgaccgcatc gatcgttttc tggattgctt ctgcgggaac agggcgttta taacatgcgc
     3061 tcaatacacc ttgttcaatc ttagaccgtt catactgctc tctgttatta tcttttttta
     3121 taacaatgag cggaatcgtc tctacttttt cgtatgtcgt aaatcttctg ccgcattcat
     3181 cacacgaacg tcttcttctg atcgagtttt tatcttctgc aggacgagaa tcaatcactc
     3241 tcgtatccgg atttccacaa tatggacatc tcatgagcat ttccgccttt ctctttccat
     3301 tttctatatg cgcaattatt ataccacacg ctttaaaatt tttgcaaaaa tttttcttct
     3361 ttaatctcca caagcacgat atccggaccg atctttttta tgcaggaaaa gggaatcaca
     3421 tattcacaat ctgtcccgaa aaatccgcat attttccccg gtccgggtat tacgatcgcc
     3481 tcgatttctc ctgtacatat gttgatctcc acatctgcga tacagccgag acgccttccg
     3541 ctgcatacat tgatcacttc tttttccttc agttcacaga tccgcatacc gcctcaccgc
     3601 cattttacgc tcttcttaat ttattatatg aacgaacagt tccaccatgc acaaagccgc
     3661 aatccatttc tctttctttt ttccagttta tgcgatacaa taacatcagt aaaacaaata
     3721 aaaatatatt tatataaaat gaaaggattt cacacatgaa agaaatgatc ttactattta
     3781 atccaccggc aaaagaacaa ctgctgaaaa tccgttctgc cctgctgcct ctgcatatcc
     3841 gcatacagct tatcaaaccg gaacagtaca atcagccgct cggatatctt gccggcatga
     3901 aagatatctc tcctgtcgaa ggaaattacg ccggagagga acttccggat acaatgtttc
     3961 tcttctcttc tttaagcagc agccgtctcg atcaggttct ggcagccctt cgcaaaagcg
     4021 gagccggccc gttcccgtat aaagccgtcc tgactgcaac taacagcaaa tggaatgctc
     4081 ttgactgctt caacgagatt cgtcgcgaac atgaagcact ccatccttaa aacacttttt
     4141 tcaaaccaat caggcagact gccgatattg ttataacttt agagggggac tatcattatg
     4201 tggaaacaaa aaacattaaa aaaatcttct aaatcatgca tgaaaccggc gtatacaagg
     4261 atggtttccg tctgtttcat tatctctttg cttacgacag cataccctgt ttccacaaca
     4321 tttttaaatc tgcagaaact ttccggtcct gcctatacag acgccgcatt ttctcttagc
     4381 attacgaatt ccgaagcgtt tgccgaaaca ctcggtctga tcttaggcgg gaacgctttt
     4441 ccaaaacttt tacaaaatac tgtatttcat gtattttctt ttgtgatcga tctatgttcc
     4501 gccggcatat ccatattttt taatgtcctt agaacagtca atgcattttt tacagaaccg
     4561 ttaggcacat cttttgtatt tcttataata ggactgtgta taacatttct ctatcaactg
     4621 tttgtgaact atgtgcttat catcggagaa atgcggtttt ttcttgaagt acgcaaatac
     4681 aaaagaactt ctatctcaaa gattttcttc ttatataaac ttcggtgtat agccgcccct
     4741 gtgtgggtca tgttctgcag atctgtattt cagtttcttt ggaatttcac gatcgcagga
     4801 gggatcataa aacattatga atatgccatg atcccgtaca tactcgccga aaatccaaaa
     4861 ataagccgaa aagacgcctt ctttttatcc aaacagctca tgaataaaaa caaatggcgt
     4921 gtgtttctgc tggactgctc tttcttagga tggaagatct tatcaatctt aacactcggt
     4981 attcttgact tcatatttgt caatccatat attacgggat gtaaggctga actttatgcg
     5041 tcgcttcgca gagactatgt cctctcgcgg tcgccgcgct atgaaatgct aagcgattct
     5101 tatttggaac atgttccttc cgaagacgaa cttcttatca gcaaagctct ttatgacgat
     5161 tcccagggac cgtatacgaa gacttcctat tttgaaccgg aacaataccc tgtctttctc
     5221 ttctccgttc agccaaagac agtaaaacct tccgcccgtg tatttcggaa ttacgatatt
     5281 ttgtcctgca tcttcctctt tcatgccgca tcctgtttcg gatggatcat ggaaatgctc
     5341 atacacctta tgcgcgacgg aactgtggcc gacatccatc tgctgttcgg cccgtggctg
     5401 cccctttacg gcatttacgg aatcattatt ctgaaagcat caaaacgcct gctcaaaaaa
     5461 ccgatttttg tatttttcct gaatttcatt gtgttttcat tcctgcaata tattttcagt
     5521 tttacggttg aacttttttc aggatacaaa ctttgggatt tcagcgaatt ctttctgaac
     5581 attaacggca gaatttatct cggaggatct gccgcatttg cgttactcgg atgtgcgttt
     5641 atttactacc tcgccccgaa ctggacaaat tatttttcaa aattatcaaa aaagacacaa
     5701 accgttttct gcgtaatctt aaattcttta tttattacgg atgtcatttt aactttgatc
     5761 ttttcatact aagttgttaa aaatagtttt atcttcctaa agcaactttt taaatttgtg
     5821 gttgatttcc atgaattgat ggtgtaatat atgcatatac ttaatgtagt ttcaaaaact
     5881 acatgttgca tttaactaat tcggaggtgc atattattat gggaaatact catgccattg
     5941 ctaaaaaatc aacctttata aaaaaacatc ctacgcttca tatacaaaat tacgcaggga
     6001 acagctattc tgccgccggt tataaaaaaa ccggttataa ttccgggaaa tccgcctctt
     6061 tgaatgaact tcgggggaaa ttttatataa aggagcccgg cagcgccatc actcatttta
     6121 tcggaatgat catggcgctt atcgccgctt tccctctgct tataaaagcc gcggcgcagc
     6181 ctgatccggt ttacctgatc tccatgtccg tttatgccgc aagtctgatc ttattatatg
     6241 cggcaagcac aacttaccac acatttgaca tttccgaaaa gggaaacact gtcttaaaaa
     6301 agatcgacca tatgatgatc tccgtcctga tagcaggaag ttacacgcct gtctgcttac
     6361 ttgtcttaga aggccgtatg cggatcattt tacttgctgt tgtatggact ctcgcattga
     6421 caggtatact gatcaaagct ttttgggtat tttgtccgaa atgggtttct tccgttcttt
     6481 atatcggaat gggatggacg tgtgtccttg catttgcaca gattttccgc aatatgcctt
     6541 tggccgcttt cggatggctg cttgcaggag gtattatcta cacagtcggc ggtattattt
     6601 atgcattgaa agtaccgatt ttcaataaca aacataaaaa cttcggaagc cacgagatct
     6661 tccacctctt cgttatgggc ggaagcatct gccacttcat tgtaatgtac ggatacctgc
     6721 ttccgtaaaa aattaaaaaa tcaacagaca tctcatctct gatttgacgt ctgttgattt
     6781 tttctgtatg accggctctt agaatcgtct tatctgcaca cgatcagccg ttcattttct
     6841 ttatatccgt ctactgtttc ttccgtcagt atcacactgc cggaaaaggg agcaaacgtc
     6901 acggcgctta tattgccgaa ctttgtactg tcggcaagaa tatacgactt ttgacactgt
     6961 tgtaccgccg tttgtttcac ctgcgcttct cctacgtccg gagtcgtaaa cccttcctct
     7021 ttcgtaatac cgtttgttcc gaaaaatcct tttgtgaaat gatattttct taaaagttct
     7081 attgcctgcg ctccgacaac agcctcagtc gaacttttca attcaccccc tataagtaaa
     7141 acacgatttc cctgagccgc cagacgctgc gcgtgtgcga ctgcgttcgt tacaaacgcc
     7201 gcccgtttct ctgccaaaaa ttctaccata tagccgactg acgttcccgc atcaagataa
     7261 acaaaatcat tgtctccgat catatctgcc gcatattgtg cgattttgat cttttcctct
     7321 ttcctgacgt tcactttttg tgccactgtc ggttctttca tctcataagt atgctcggca
     7381 gcaaccgctc cgccaaacac tttcgtcagc ttccccgcct tatcaagcgc agtaatatcc
     7441 ctccttgccg tggattccga aattccgaga tcttctgtaa tttcagctac tgtaatactt
     7501 ttctttactt tcagccgttc taatatccgt tcaaaccttt gctctgttaa cataatgccc
     7561 tctgtgcata taataaacta tactatatgt tctttctctt tttccaatac gagggtcatc
     7621 tttcaatcta tattttttga tgaccctcgt atttactatt tgacactatt ctatcatttt
     7681 ttcactttat atgcttcact tttttttgct ttttcttatt tttctgtttc ccgctgagga
     7741 caaacagttt tttattcttt atatttttat actttttaag ctttcttttt caatactccg
     7801 agcagcaatg ccgacacaac tgttcccgca acaagcgcga tcagatattg aagcggattt
     7861 cctacaaccg ggaatacgaa gattccgccg tgcggcgcca tcagcgtaca gttaaacgcc
     7921 atggaaagcg ctcctgcaac cgccgatcct gcgatacagg ccgggagcac atgaagggga
     7981 tcagatgcag cgaacgggat cgctccttct gtaatgaaag caagacccat aataaagttt
     8041 gtcggacctg cgtctctttg ttcttttgta aatttgtttt taaagagcat tgtcgcaagc
     8101 gcgatcgcgc acggcgccgt cattcctccg accataaccg ccgccataat gtcataattt
     8161 cccgccgcga tagacgcagt gccgaacaca tatgccgcct tattaaacgg acctcccata
     8221 tcgatcgaca tcattcctcc gacgatcatt ccgagaagga tcttgctcgt tccgctcatg
     8281 ctggaaagtc ccgcattcaa agcggtgttg atgcctccca taaccggttc gaccgcaaac
     8341 gtcatcagta atcccatgat caaaatacca aataccggat acaataatac aggcgcgatt
     8401 ttctcgactg cttcgggcag cttgccgcac acttttttca gacctacgat aatgtatcct
     8461 gccgcaaatc ctgctacaag cgctcctaag aatcctgatt ttccgcctgc tgcgatcatt
     8521 ccgcctacaa atccgactgc aagcgccgga cgatctccga tcgccatcgc gatgtagccc
     8581 gcaagcaccg gaagcataaa tccaaacgca actcctccga tctgtttaaa caaagccgca
     8641 gccggcgtga tcgtaccaaa agccgctctt tcatccgccg gaagagaatt aaggtcgata
     8701 cttaaaccgt cgatcaaaaa tgcgatcgcg atcaggattc ctcctcctac tacaaacgga
     8761 agcatatgag aaacgccgtt cataagctgt gtataaattt tatgtccgat tcctcctgat
     8821 tttcctgacg aagacaccgc tgtcgaagag gacgtttcgt ttccgctgtg atatacagga
     8881 acgttgccgg acattgcctg atcgataagc tgaggcgctt tactgattcc gtctgatacc
     8941 tgacagatga tcactttctt tccgtcaaaa cgatccatcg gaacttgcgc gtctgccgct
     9001 acaataatac agtccgcttc tttgatctct ttctctgtaa gaacgttttt cgctccgccc
     9061 gagcctctcg tctcgatctt cacgaaacaa tctttttcct tcgccgcttt ctcaatacct
     9121 tccgccgcca tataagtatg ggcaattcct gtcggacagg acgttacggc aagaatttta
     9181 aatccgtttg cagaagcttc gttcatatct gcaagacgct cgtcaatatc gccctgctcc
     9241 gcatctgcct tatcgataat atcaagaaat tcatcggcgc tttttgcatt tctcagcgca
     9301 tcggcaaata cttcatccat catcagtacc gacagcttgc tcaacacgtc caaatgtacg
     9361 ttatctttcg tattcggcgc tgcgatcaaa aacatcagcg taaccggctg tccgtccaat
     9421 gcgtcgaatt ctactccgtc ttttactacc attgccgcaa gacccggttt cgttaccgca
     9481 tcacattttc catgaggaat ggcaattcct tctccgattc ctgtggtgct ttcttcttct
     9541 cttgcgtata cttgttctct gtatgcttca cggtcgttga tctttccgct tgcaaccata
     9601 agatcaataa tcctgtcaag cgcttcactt ttcgttttcg gagtctcgct caacgaaata
     9661 ctccttttat caagcagatc tgtgatcctc attctcaaaa cctcctgttc atcttcgttt
     9721 tcttccgttt atttttttgc ttatttgctt ctgtttattt atgtttgatt ctgtttgctt
     9781 atgtttggct ctgtttgttt ctgtctaact ctgtttgttt ctatttagtt ctgtttgttt
     9841 ctatttatta ctgtttactt ctgtttgttt ctgtttactt ttcttttctg cttacatctt
     9901 tactgtacac agttcgtttt tattcatttt gaaacctttt cgtacagatc catgatttct
     9961 tcttttgttg caagatattc agaaaatgca cttgcacttc ctgcggatac ccccatataa
    10021 aatgcgtgtc tgtaatcttt cttctcgatc catcctgccg taaatccggc aaccatggag
    10081 tcgcctgcac ctactgcatt taccaaagtt ccttttggcg ccggagcttc atacacactg
    10141 ccgtcttccg ctacgagtac cgcgccttca cctgccatag agatcagaac gtttctcgcg
    10201 ccctgctttt gaagtttttt cccgtaagga atcacctctt ctcttgtcgt cagtttgaca
    10261 tcgaacaatt ctcccagttc atgattattc ggcttgatca aaaacggctt gtattttaat
    10321 acattaataa gcaaatcctt tgtagcgtct acaataaacg ttacattttt atgctgaagc
    10381 cgttccatga tcgtactgta catgtccgcc ggcatcgatg ccggaatgct tcccgcaaga
    10441 acaagaatat ctccctctcc caaaatatcc agtttcctca atagttcttc gatcttctct
    10501 tcgcttattt ccggccctga accgttaatt tccgtaccgt caatattttt cagtttcaga
    10561 ttgatccgtg aaatcccact gtcgatctta ataaaatcag aacggattcc catcgcttct
    10621 acttctctga taatctcttc tcccgtaaat cctgccgcaa atccaaacgc tgtactgtca
    10681 attccgagat tcattaaaat tgtcgataca ttgattccct ttcctccggg aaggatcaat
    10741 tccgaatccg tccggttcgt aagtccaagc tgaaaatcat ttacagaaac gatataatcc
    10801 aaggacggat taaatgtaac tgtatagatc atctccattc ctccttttct ttttgcgtct
    10861 ttttgttttc ttgatgtcat tatataatca tattcagtca aattcaatca taataattca
    10921 catagttttc attttatttt cgttgaattt gcacaaatat aatctttttt gacttatttt
    10981 gaccgaatta aatataataa ccggacagaa cacaaatatt tatctacatt ctgcccggtt
    11041 atttatagtt atttgtattc atttatatct gagcaaacag atctctgtcg cgtcagacat
    11101 cctgcttgtc ccatgacatg tctatctcag acgatccgaa atttttctcg atcaaaccgc
    11161 aatactcttt atactcttcc tcttcggaaa agttttcttt taaagcgtcc cgaacccctt
    11221 tataatacca ctccaacgcc gctttgcttt tcatccgaaa acgctcccat aacttctctc
    11281 ctacaaccgg ataatctctg tcgatatccc gcatatttga gagcttatct gcaagaccga
    11341 tcaactgtac ttctctttgt gcgtctttta atctggcgat cgtcgcgctc ttgcgttctt
    11401 tccagctaag cgtcttatcc tcactttcct gcgctacaag ctctgcaact ctgtctccga
    11461 aacggagctt caaaacgtct tttgtaatcc ccctgcagtc ttcaatcgta tcatgcagca
    11521 ccgccgcaca tatcacttct tcatcatctg tcattgccga tacaatctct gacacctcta
    11581 tcggatgaac aatataaggt ttcttcgtac cttttctcaa ctgtccctcg tgtgcctttg
    11641 ccgcaaattc gatcgcctca tttatcatac cgcatcctcc gctttcttaa ttttcgcaca
    11701 attcactttt ctcgataaat ttatttataa attttagcac aaccgtaatc taagtacaag
    11761 acaattacga cgttcagctt ttcctcattt gcgcctctta atttttcctg atatactgtt
    11821 ctctgacaat caattatttt gaaaggtacg aaaacaatga agaaaaaata tgtccttatc
    11881 accggatctt ccagaggaat agggcgcgcc tgcgccgtcg cctttgccaa aaacggttat
    11941 catgtattta taaattgtaa aaactcagtc gaacaactgg ctgaaaccga acatgaaatt
    12001 cttgcttccg gcggcggctg cacagctctc cccggcgatg taagcgatcc cgacgttgtc
    12061 cgcagcatat tttgcaaaat aaaaaaagtc tgccaaggcg ttgatgtcct cgtcaacaat
    12121 gccggaattt cccatttcgg cttattaagc gatctttccg actcggaatg gaaaaatctg
    12181 atcgacacaa acctttcttc tgcattttac tgctgcagag aagctatccc tcatatggta
    12241 tctgaaaaat gcgggaaaat catcaatatt tcttccatgt ggggaacggc gggcgcttcc
    12301 tgtgaagccg cttattctgc ttccaaatca ggactccacg gcctgacaaa agctttggca
    12361 aaagagcttg cgccaagcgg catttcggta aacgccgttg cctgcggcgt cattgacaca
    12421 gatatgaact cccgcttaaa tgaagaagaa aaaaatatgc tttgcgaaga aattcccgcc
    12481 ggcagattcg gaacttgtga agaagttgca aggacagtcc ttgcccttgc cgaaagtccc
    12541 gcctatctca ccgcacagat tataggcatt gacggcggat ttttataaac aaagcacaaa
    12601 atggatcaat cccgaaagta ccaaaaggca cattccggga ttgatccatt ttgtaacatt
    12661 acttatctga gcgcatattt tatatagatc agataaaaaa ttcaggagga tattactttg
    12721 ccgtactcaa tcatgcttga cgccggacat gggggacgcg atccgggagc tgtatacaac
    12781 ggaagacagg aaaagatcga tacactttct cttacacttg ccatcggaca gattctgcag
    12841 gagcggggaa tcgatgttct ctatactcgc acgacagata tttacgagtc gccttatcaa
    12901 aaggcaatgg aagcaaatga agccggagtc gattttttca tctctatcca tcgtaattct
    12961 tttccgcaag ataacgctgt ctcgggagtc gaatctcttg tctacgacaa atccggcatt
    13021 aaactggaga tggcggaaaa tataaacgaa caacttgagg ggatcggttt tgtaaatctc
    13081 ggagtaaaag cccgccccgg gctcgttgtc ctccgacgca caaaaatgcc tgcggttctt
    13141 gtcgaagtcg gatttatcaa ttctgacacg gacaacatgc tctttgacga taacttcagc
    13201 gatatcgccc ttgccatagc tgaaggcatc ctcgatacac tcggcatcaa tacccctgtt
    13261 gttcctcttc cggatgaaaa tgaagctccg gtcgctccgc tgcctgatga aaacacatat
    13321 cctgatgatg cctttcctaa tgaagacaac gttcctgtcg ttcctcttcc gcctcgctac
    13381 acagttcaaa caggcgcatt tcgtaatccc ctgtatgccg aacgactccg ccgggagttg
    13441 tccgaacttg actttcctgc ctctgtcgtc gagggaaacg gattataccg agtcatggta
    13501 ggcgttttcc ccacacttca cgatgcggca gaaacagaac gtcggctgcg aagcgccggt
    13561 tatcaaacag tcatcgtcag ccaataaata aaactaaaac gtaccctaat gatccgacac
    13621 gcttcttgat atgcgttttc ttttattagg gtacgtttta tatttcatat tatcaggtat
    13681 ctatcataca ttatctatgg attttcccca tccggctctt ctgctaatgc ctgctcatat
    13741 ttttctaaaa tcatagactg aatccgttct ctcgtattag aattgatcgg atgtgcaata
    13801 tctctgtact caccgtccat tgctttttta cttggcatgg caatgaacat tcctttctct
    13861 ccttctataa ctttaatgtc atgaacaaca aattcatcgt cgatcgtaat ggatacaacc
    13921 gcttttaatt ttccctcttt cgcaactttt cgcactcgta catctgtgat ctgcataggt
    13981 tcagatctcc tttcttttct gccttttcgg agtattacgc cgctttttta caatgc
//
LOCUS       DBMDXF010000028        13918 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1286_length_13918_cov_2.160382, whole genome shotgun sequence.
ACCESSION   DBMDXF010000028 DBMDXF010000000
VERSION     DBMDXF010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13918)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1286_length_13918_cov_2.160382"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            129..407
                     /locus_tag="ACZPD4_02195"
     CDS             129..407
                     /locus_tag="ACZPD4_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HIR1377922.1"
                     /translation="MFLSFAETFKALSDPVRREILELLKKGRMSAGEIASHFDMTQAT
                     VSYHLKILKKADLIRETREKNFIFYELSLTVLEEIMVWISDLKGEKET"
     gene            404..1054
                     /locus_tag="ACZPD4_02200"
     CDS             404..1054
                     /locus_tag="ACZPD4_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIR1377923.1"
                     /translation="MKKYKTTIIITTLITLLPIVIGLFLWNKLPDTLATHWGSDGTAN
                     GWSSKTFSVFGMPLILTAIHVLCLFVTLNDPKKKNIHKKPMALIFWLVPVISLLANCS
                     TYLIALGVDFSIEIFISALIGVFFIVLGNYMPKLQQNYTIGIKLPWTLNSEENWNRTH
                     RLGGKLYIVMGIAMILMSFIDSLLGSEAAAWIILSVITIGAIIPAAYSFYLYKKGI"
     gene            complement(1224..2126)
                     /locus_tag="ACZPD4_02205"
     CDS             complement(1224..2126)
                     /locus_tag="ACZPD4_02205"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1377924.1"
                     /translation="MNTKVTIAGVEWNNPVTVASGTFGSGAEFTDFVDLNRLGAVTTK
                     GVANVPWTGNPTPRVAETYGGMMNAVGLQNPGIDVFCERDIPFLRKYDTKIIVNVCGR
                     STEDYCEVVERLANEDVDMLEINISCPNVKEGGIAFGQNPNAAEEITKAVKKYAKQPI
                     IMKLSPNVTSISEMAKAVEAGGADAVSLINTLTGMKIDVNKKAFVLANKTGGVSGPAI
                     HPIAVRMVYEAANVVSVPVIGMGGIASAEDAIEMILAGASAVSVGTANFFDPAVTMKI
                     VDGIESYMKQHGFESVADMVGIVK"
     gene            complement(2126..2908)
                     /locus_tag="ACZPD4_02210"
     CDS             complement(2126..2908)
                     /locus_tag="ACZPD4_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1377925.1"
                     /translation="MTKEKENAAIISQEEIAHGIYSMWIRTKAAETAEPGQFISMYTN
                     DGSKLLPRPISICEIDKEKGALRVVYRVTGENTGTELFSQMGEGDTIPVIGPLGNGFP
                     LEKALEKRAFLIGGGIGVPPILELAKQIGCEKKQIIVGYRNDETFLKTEFEQNGEVYI
                     STEDGSAGTKGNVMDVIRENGLEADIIYACGPTPMLRAIKNYAEENGIECYISLEERM
                     ACGIGACLACVCKTKEKDHHSNVHNKRICKDGPVFLSTEVEI"
     gene            complement(2997..3926)
                     /gene="pyrF"
                     /locus_tag="ACZPD4_02215"
     CDS             complement(2997..3926)
                     /gene="pyrF"
                     /locus_tag="ACZPD4_02215"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1377926.1"
                     /translation="MINKLVEKIKKTKAPIVVGLDPMLSYIPQHVQEKAFAEYGETLE
                     GAAEAIWQFNKEIVDKTYDLIPAVKPQIAMYEQFGVPGIAAFKKTVDYCKSKDLVVIG
                     DIKRGDIGSTSAAYAVGHLGKVKVGSKEYVPFDEDFATVNPYLGSDGVNPFADVCKEQ
                     KKGLFILVKTSNPSSGEFQDRIIDGRPLYEHVGEKVAQWGAECMGDEYSYIGAVVGAT
                     YPEMGKVLRKIMPKSYILVPGYGAQGGRGKDLVHFFNEDGLGAIVNSSRGIIAAYKQE
                     AYAKFGAENFGDASRAAVEDMIADISGALESAK"
     gene            complement(4305..4748)
                     /locus_tag="ACZPD4_02220"
     CDS             complement(4305..4748)
                     /locus_tag="ACZPD4_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR1377927.1"
                     /translation="MLELLRKKKSLRILIVAGAAIAALAGAVYLYFHNPHSYPLPCVF
                     YSLTGFYCPGCGAGRASYSLLHGRFLDAFCYNPVMTVLLPLLALYVAARAIDWAITGG
                     DHIDQKISVKFVAGILIFVLLFGIVRNIPVYPFNLLVPGGLFELF"
     gene            complement(4771..5511)
                     /locus_tag="ACZPD4_02225"
     CDS             complement(4771..5511)
                     /locus_tag="ACZPD4_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HIR1377928.1"
                     /translation="MNCIKCYQEIPDGAKFCPYCGAQQSDTSATDPVRAEETVTEPVQ
                     AAQTEEVPTEPVQAAQTEEVPTEPVQAAQTEEALTEPVQPTQTEEAPTEPIQPEAAST
                     DHVYQEAPAQETYTSAQNNPYYGTGQTYSDQTSDQQYGGNQGQTSYQNQMPYQNQAPQ
                     TPINWVPYLVLSIICTLCCCLPFGIVGIVYAAKINSSMAAGNYEEAKNAAKSAKIWII
                     ASAVVGLIVEVIYIIFAFIGGVGGYYYY"
     gene            complement(5623..6243)
                     /locus_tag="ACZPD4_02230"
     CDS             complement(5623..6243)
                     /locus_tag="ACZPD4_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1377929.1"
                     /translation="MTKKNETVVKIAQTALMAALCFVSFQFLQFKIVLPGGDATSFHV
                     GNAFCVLAALILGGWYGGLAGAIGMGFADILDPVYITGAPKTFVLKLCIGLIVGLVAH
                     RIAKINESTDKKYIFRWSMIASVAGLGFNVIADPIVGYFYKQYIMGQPQQMAEVLAKW
                     SAATTFVNAVISVILAGVLYNALRPVLLKTGIIRQKREEAVTAKSI"
     gene            complement(6348..9113)
                     /locus_tag="ACZPD4_02235"
     CDS             complement(6348..9113)
                     /locus_tag="ACZPD4_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1377930.1"
                     /translation="MENTSEKKDKNSVKKPLKHICIGLVAHVDAGKTTLSEGMLYLSG
                     QIRNMGRVDHGDTFLDNYETERERGITIFSKQAEFIWNDTSITILDTPGHVDFSAEME
                     RVLQVLDCAVLVVSAVDGVQAHTVTLWRLLKQYKIPTMIFVNKMDRQEADREKLLEEL
                     KNRFGDGCVEMDMQSEENRESLAMCDETMLEEYLSGGKIEKETVKKAVAARKVFPCWF
                     GSALKAQGIEALMSGLCEYAPSAEPLPEFGAKVYKIARDPQGNRLTYLKVTGGQLRVK
                     DVPFAAEQSCTGKVNQIRKYSGEKYELVQTVSAGEVCAVTGLEGTYPGQGIGAQKESL
                     LPVLEPVMTYRIELPDGCDAHKMFQNLRCLEEEDPQLHVIRNEETSEIHIRLMGEVQT
                     EVLQKMVKDRFGVLIHFGEGRIVYKETIKNSVEGAGHFEPLRHYAEVHLRLEPGERGS
                     GMQFAADCSEDVLDRNYQRLILTHLEEREHKGVLTGSALTDVRITLLSGKAHKKHTEG
                     GDFRQATYRAVRQGLRKAESVLLEPYYEFRMELPLENVGKAMTDIKRMSGEFEGPETE
                     NGMAVLKGSVPAAEMNGYQKEFTAYTGGYGRLFCSLKGYGECHNTEEVIGQIGYDADA
                     DVENTADSVFCSHGAGTIVPWYEADAHMHVEGEAAEKSEEDTQMSAAFRPQRRTIELT
                     QEELDAIYVRTPDPVKKTKRSAPVTVTAGKAAAFCNGDRLQSRNVPFDISGDYTKTKK
                     KKADRKEYLLVDGYNIIFAWDSLKELAKIDLAAARGKLADILCNYQGYKQCELILVFD
                     AYKVEGNPGEIMKYHNIHVVYTREAETADHYIEKTVRRIAKEHDVIVATSDALEQVII
                     MGQGAHRMSAAGLKEEIESVIEQMKDDYPKQEELIRNYLFDYLEKDIAEEMERVRLGS
                     HKKKWTV"
     gene            complement(9215..9637)
                     /locus_tag="ACZPD4_02240"
     CDS             complement(9215..9637)
                     /locus_tag="ACZPD4_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR1377931.1"
                     /translation="MRAEFDESLVTGNEMIDTQHKELIDKINKLLDSCETSKEKVVAV
                     KTLDYLADYTEFHFGEEEKLQESINYPAIAEHKKEHDKLRAVVKDLYNMLEEEEGPSE
                     AFVEQVNKNVIEWLYRHIKGFDRSVAEYKFMNESSERL"
     gene            complement(9642..10502)
                     /gene="nudC"
                     /locus_tag="ACZPD4_02245"
     CDS             complement(9642..10502)
                     /gene="nudC"
                     /locus_tag="ACZPD4_02245"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR1377932.1"
                     /translation="MIQDIEPHHLDNQYKPVPPDKDSIALYYEDHAALMKVSEKGISF
                     PSFADLEHLNREIYKECIYLFSVDDERYYLVNEIDRSMLLDFQMKNTEIFRTADPQDR
                     AFAGITGYQLYNWYKDRKYCGRCGKRLKHGEKERMLVCEACGNTEFPKICPAVIVGVT
                     DHNRILMSKYAGRTYKKYALLAGFSEIGETVEQTVAREVMEEVGLKVKNIRYYKSQPW
                     AFSDTLLMGFYCDLDGNDAITLDREELAVAEWFEREDIPVEPSRDSLTNEMILQFKNE
                     RTAVKKEKGE"
     gene            complement(10553..11017)
                     /gene="smpB"
                     /locus_tag="ACZPD4_02250"
     CDS             complement(10553..11017)
                     /gene="smpB"
                     /locus_tag="ACZPD4_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR1377933.1"
                     /translation="MAKSEVRMIANNKKAYHDYFILEKYEAGIVLHGTEVKSLRMGKC
                     SIKEAFIRVESGEMYIYGMHISPYEKGNIFNKDPLRVRKLLLHKKEIDKMFGKMKEQG
                     ITLVPLQVYFKGSLVKMEIGVAKGKKLYDKRDDIAKKDQKREAQREFKIRNL"
     gene            complement(11119..13326)
                     /gene="rnr"
                     /locus_tag="ACZPD4_02255"
     CDS             complement(11119..13326)
                     /gene="rnr"
                     /locus_tag="ACZPD4_02255"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR1377934.1"
                     /translation="MRKTHGNREKKRRIHDDRVKVNDRKNPSKSKEESRQFEKRKKVL
                     FDFINDDLYVPMKIKEIAVVLQIPGEQRQELREVLDALVEEGKITISKRGKYTCGHTE
                     RLKGIFQANARGFGFVTPEDGTDDIFIPEECLGNAFQGDEVEYIITSAPAGKRREGKI
                     VGIISHGVTHIVGLYEKCKSFGFVRPDNCRYVNDIYIPSGREMGAVTGHKVVVEMTSY
                     GGEHMKPEGKVTQIIGHVNDPGTDILSIVMDLGIKTEFPEKVLNQAVRVGKPVSEADR
                     AGRKDLTNVKMVTIDSEDAKDLDDAVSVERDGENYVLGVHIADVTNYVQEKSALDREA
                     LERGTSVYLADRVIPMLPHILSNGVCSLNAGEERLALSCIMTISPKGEMISHEITESV
                     ICVDKRMSYNKVSAVLEQDEQALREYEGFVPMIWMMKELSELIRQKRGKRGAIDFDFP
                     ETKIVLDEDGKPIEVKPYDRNPATEIIEDFMLMANETVAEEFFWREIPFLYRTHEAPD
                     EDRIRQLSAFVNSFGYHIHVGNEIRPKEIQKLLEKVEGKPEEDLISRLTLRSMKQARY
                     TTENTGHFGLAAKYYTHFTSPIRRYPDLQIHRIIKENLRGRFDENRAEHYSELLQQVA
                     AQCSERERRAEEAEREVVKLKKAEYMRDHIGEEFDGVISGVTKWGAYVELENTVEGLA
                     HVADMWDDHYEFYEQSYELVGEHTGKTYKPGQKVRICVTDADKLQRTVNFRIL"
     gene            complement(13512..13754)
                     /gene="secG"
                     /locus_tag="ACZPD4_02260"
     CDS             complement(13512..13754)
                     /gene="secG"
                     /locus_tag="ACZPD4_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1377935.1"
                     /translation="MDILKTVLTIIFVIDCIALACIILLQEGKSAGLGTISGAADTYW
                     GHNKGRSMEGALVKATRVIAVLFIVLAVVLNLNIFN"
BASE COUNT         3420 a         3739 c         2359 g         4400 t
ORIGIN      
        1 ggctatggga tttcacagaa aacattttga gaatttagat ttttttctaa atagatattg
       61 actttttatt tccgcagtgc tctaatatat ttagaaatat ttctaaatac tatttaaagt
      121 aagggggaat gtttttgagc tttgccgaaa cgtttaaagc attatccgat ccggtaagac
      181 gggaaattct ggaacttctg aaaaagggca gaatgtctgc cggagagatc gcaagtcatt
      241 ttgatatgac gcaggcgact gtctcctatc atctgaagat tttgaaaaaa gcagatctta
      301 tcagggaaac acgggagaaa aattttattt tctatgagct gagccttacc gtattagagg
      361 agattatggt atggatctca gacttgaaag gagaaaaaga gacatgaaaa aatataaaac
      421 cacaatcatt attacaactt tgatcacatt acttcctatt gtgatcggac tatttctttg
      481 gaacaaactt cccgatacac tggcaacaca ttggggaagc gacggaaccg caaacggatg
      541 gtccagcaaa acattttctg tcttcggaat gccgctcatc ctgactgcga tccacgtttt
      601 gtgccttttt gtcactttga atgacccgaa gaaaaagaat atccacaaga agcctatggc
      661 gcttattttc tggctcgttc ctgtgatttc tcttttggct aactgttcga cttatctgat
      721 cgctttagga gtagatttca gcattgaaat cttcatatcc gctctgatcg gtgtcttttt
      781 catcgttctc ggaaactata tgccgaaact tcaacaaaac tatacgatag gcatcaaact
      841 tccgtggaca ttaaacagcg aggaaaactg gaatcgaacc catcggctcg gaggcaaact
      901 ttatattgtt atgggaattg ccatgatttt aatgtcgttt atagacagct tactcggaag
      961 cgaagccgcc gcatggatca tcctgtccgt gatcactata ggcgctataa ttcctgccgc
     1021 ttattctttc tatttgtata aaaaaggcat ttaaatgaat cttctattat aaaaataacg
     1081 tctgtcagaa tttttgatcc tgacggacgt tatttttgtc ctcttatact cgttttctta
     1141 tgctcgtttt cttatattcg ttttcttata ttcgttttct tatattcgtt ttcttattct
     1201 cttttcttat gtttgctttt catctacttt acaattccga ccatatccgc aacactttca
     1261 aatccatgtt gtttcatata tgattcaatc ccgtctacga tcttcattgt cactgcagga
     1321 tcaaaaaagt ttgctgttcc taccgatacg gcgcttgccc ccgcaagaat catttcgatc
     1381 gcatcttccg cagatgcaat tccgcccatt ccgattacgg gaacactgac gacatttgcc
     1441 gcctcatata ccatgcgcac agcaatcgga tgaatcgccg gtcctgacac tccgcctgtt
     1501 ttgtttgcca aaacaaacgc ttttttattc acatcaatct tcatccccgt taaagtattg
     1561 atcaaagata cggcgtctgc gccgcctgct tccaccgctt tcgccatctc ggaaatactt
     1621 gtaacattcg gacttaattt catgatgatc ggctgttttg catatttttt tactgccttt
     1681 gtaatctctt ccgcagcgtt cggattctga ccgaaggcaa tcccgccttc tttcacattc
     1741 ggacaggaaa tattaatctc cagcatatcg acgtcttcat tcgccagacg ttctacgact
     1801 tcacaataat cttctgtgga tcttccacat acatttacaa tgatcttcgt gtcgtatttt
     1861 ctcaaaaacg gaatatccct ctcgcaaaac acatcgattc ccgggttctg caagccgact
     1921 gcgttcatca ttcctccgta agtttccgct actctcggcg tcggatttcc tgtccacgga
     1981 acattagcaa cgccctttgt cgtcacagct ccgagtcggt tcagatcgac aaaatcagta
     2041 aactctgctc ctgatccgaa cgtgccggaa gcaactgtaa ccgggttatt ccattccact
     2101 cctgcgatcg tcacttttgt attcatcaga tttccacctc cgttgacaaa aataccggac
     2161 cgtctttaca gattcttttg ttatgcacat tactgtgatg atctttttct ttcgtcttgc
     2221 atacacatgc aagacatgct ccgataccgc atgccatgcg ctcttccagc gaaatatagc
     2281 attcgatccc gttttcctcc gcatagtttt tgatcgcgcg gagcatcggt gtcggtccac
     2341 acgcatagat aatatctgct tccaatccgt tttcacggat cacatccatt acattgcctt
     2401 tcgttcccgc acttccgtcc tctgtagaaa tatatacttc cccgttttgt tcaaactctg
     2461 ttttcaaaaa cgtctcgtca tttcggtatc cgacaatgat ttgctttttc tcacagccga
     2521 tctgttttgc cagttccaga atcggaggca ctccgatccc tccccctatg agaaacgccc
     2581 gtttttctaa tgctttctcc agcgggaatc catttcccaa cggtccgatc acaggaatcg
     2641 tatcgccttc tcccatctgt gaaaaaagct ctgttcctgt attttctcct gtaacacggt
     2701 atacaacgcg cagtgcgcct ttctctttgt ctatctcgca aatactgatc gggcgcggaa
     2761 gaagtttgct tccgtcgttt gtatacatcg agataaactg ccccggctct gccgtttccg
     2821 ccgctttcgt tctgatccac atactgtata tgccatgcgc gatttcttcc tgtgaaatga
     2881 tcgccgcatt ttctttttct ttcgtcatat gtgtcatatg ctcctttatt tcaaattctc
     2941 tattacaaat tatcaactgc tttttccgtc agatcgtctt tgtccctgcc ggatacttat
     3001 tttgcagatt caagcgctcc gctgatgtcc gcgatcatat cttctacagc cgctctggaa
     3061 gcgtctccga aattctctgc tccgaacttt gcatatgctt cctgcttata tgccgcaata
     3121 attcctctcg acgagttcac gatcgctcca agtccgtcct cattaaagaa atgaacaaga
     3181 tcttttccgc ggccgccctg cgcgccgtac cccggaacga gaatatatga tttcggcatg
     3241 atctttctga ggacttttcc catctccggg tatgtcgctc cgacaacggc gccgatataa
     3301 ctgtattcat ctcccataca ctctgcgccc cactgcgcaa ctttttcgcc tacatgttca
     3361 tacagcggac gtccgtcaat gatgcggtct tggaattctc cgctggacgg attcgatgtt
     3421 ttcacaagaa taaataaccc ttttttctgc tccttgcata cgtccgcaaa cggattcact
     3481 ccgtcagatc caagatacgg attcactgtt gcaaaatctt cgtcaaacgg aacatactct
     3541 tttgatccga cttttacctt tccgagatgt cctaccgcat atgcggctga cgtagaaccg
     3601 atatctcctc ttttaatatc tccgatcaca acaagatctt ttgatttaca gtaatctact
     3661 gttttcttaa atgcggcgat tcccggaaca ccgaactgct cgtacatagc aatctgaggc
     3721 ttcacagccg gtatcagatc atatgtctta tctacgattt ctttattaaa ctgccagatt
     3781 gcctctgcag ccccttccaa agtttcccca tactctgcaa acgctttttc ctgaacatgc
     3841 tgaggaatat agctaagcat cggatcaaga cctactacga tcggcgcttt tgtcttttta
     3901 atcttttcta ctaatttgtt aatcatacaa gtaccctccg ttttcctgaa aatctgcctt
     3961 gcttttgtgt aaagcagatg cctcacacat tttcagaact tttttatctt ttatatttta
     4021 tcacaatatc cgactgactt ctataccaaa atcttgcctg cctcatccct gaaatgccaa
     4081 aaggaagatt ccgagattgc aaatgatcgc caagattcct ggcagatccg gactttatcc
     4141 tgtcgccgcc gtaaaaatat aagcggacac tctcggaaat tccttaagta tccgcttata
     4201 tatttttaat catttctcat ttatctttcg ttcttccgct ttatccggct tacataatag
     4261 cttttttcgc ctcttgtttt ccggccttcc tgtgagccgc aaatttaaaa cagttcaaac
     4321 aggccgcccg gcacgagcag attaaacgga tataccggaa tatttcttac gattccgaac
     4381 aaaagcacga aaatcaatat tcctgcgaca aattttacac tgatcttctg atcgatatga
     4441 tctccgcccg taatcgccca atcgattgct cttgccgcca cataaagcgc taatagcggg
     4501 agcaataccg tcataaccgg attatagcaa aacgcatcca aaaaccgtcc atgcaaaaga
     4561 gaataactgg cgcgtcccgc gccgcatccc ggacagtaaa accctgtcag agaataaaaa
     4621 acacacggca gcggatagct gtgcggatta tgaaaataca agtaaaccgc tcccgccagc
     4681 gccgctatag cggctcctgc tacgatcaag atccgcaaac ttttcttttt acgcaataat
     4741 tctaacatgt atttcctctg ctatagcatc ttagtaataa tagtatccgc ctacgccgcc
     4801 gataaaagca aagatgatat agatgacttc tacaataaga cctacgactg cagatgcaat
     4861 gatccagatc ttcgcggatt ttgccgcatt tttcgcttcc tcataattgc ctgccgccat
     4921 agatgaattg atctttgccg catatacgat acctacgata ccgaacggaa gacaacagca
     4981 caacgtacaa ataatagata atacaaggta cggtacccag ttgatcggcg tctgcggcgc
     5041 ctgattctga taaggcatct ggttctggta agatgtctga ccttgatttc ctccatactg
     5101 ctgatctgac gtctgatcag aataagtctg acctgttccg taataaggat tgttctgtgc
     5161 ggatgtatat gtctcctgtg ccggagcttc ctgatatacg tgatccgttg aagcagcttc
     5221 cggctgaatc ggttctgtcg gagcttcttc tgtctgggtc ggctgtaccg gctctgtcaa
     5281 agcttcttct gtctgtgccg cctgtaccgg ctctgtcgga acttcttctg tctgcgccgc
     5341 ctgtaccggc tctgtcggaa cttcttctgt ctgcgccgcc tgtaccggct ccgtcactgt
     5401 ttcttctgct cgaaccgggt ctgtcgctga cgtatcagac tgctgcgctc cgcaatacgg
     5461 acaaaacttt gcgccgtcag gaatttcttg ataacactta atacaattca ttttcctacc
     5521 tcgctctcat agtatttgta tattatttca tttcaataat aataccactg tcagagcaaa
     5581 aaagtcacca aaaaaataca tttttttgct ttttttacat tttcatatac tcttggctgt
     5641 caccgcttct tcccttttct gccgaatgat ccctgttttc agaagaaccg ggcgaagcgc
     5701 gttatacaaa acgcctgcga ggatcaccga aatcactgca tttacaaaag ttgtagccgc
     5761 actccacttt gcaagaactt ccgccatctg ctgcggctgc cccataatat actgtttgta
     5821 gaagtatcct acaatcggat cggcaatgac gttaaatcca agccctgcga cagatgcgat
     5881 catgctccag cggaaaatgt atttcttatc tgtactttca ttgatcttcg caattctatg
     5941 agccacaaga ccgacgataa gtccgataca taatttcagt acaaacgtct tcggcgctcc
     6001 tgtaatatag accggatcga gaatatctgc gaatcccatt ccgatagctc ccgcaagtcc
     6061 gccgtaccat ccgcccaaga tcagagccgc cagtacgcaa aatgcatttc ctacatgaaa
     6121 agacgttgcg tcgcccccgg gaagaacaat tttaaactgc aaaaattgaa atgatacaaa
     6181 acaaagcgca gccataagag ctgtctgggc aatcttaact acagtttcat tttttttcgt
     6241 catatactta cctgctttcc tgaactgtta cttttttgtt caacattatt taatacaggt
     6301 atattagcat cagactggtc gtattgaaac atccagttat cccattttta tacagtccac
     6361 tttttcttat gagaaccaag tcggactcgc tccatctctt cggcaatatc cttttcaaga
     6421 taatcaaaca agtaattccg gatcagctcc tcctgtttcg gataatcgtc tttcatctgt
     6481 tcaataaccg attcgatctc ctctttcagt cctgccgccg acatccgatg cgctccctgc
     6541 cccataatga tcacctgttc aagcgcatcc gatgttgcca cgataacatc atgctccttt
     6601 gcgattcttc gcacagtctt ctctatataa tgatccgcgg tttccgcctc cctcgtgtaa
     6661 acgacatgaa tattatgata tttcatgatc tctcccggat ttccttctac tttatacgcg
     6721 tcaaatacga ggatcagttc acattgtttg tatccctgat aattacacaa aatatccgca
     6781 agcttccctc ttgcggcggc cagatcgatt ttcgccaatt ctttcagtga atcccacgca
     6841 aaaataatat tatacccatc tacaagcaaa tattctttcc ggtctgcttt cttttttttc
     6901 gtttttgtat aatcaccgga aatgtcaaaa ggtacatttc gggattgtaa gcggtcgccg
     6961 ttgcaaaaag ctgccgcctt tcctgccgtc acagtaacag gcgcgctcct tttcgttttc
     7021 ttcaccggat cgggcgttct gacatagatt gcatccagct cttcctgtgt cagctcgatc
     7081 gttcttctct gtggacgaaa tgctgcagac atctgtgtat cttcttccga tttttctgcc
     7141 gcctcgcctt ctacatgcat atgggcgtcc gcctcatacc acggaacgat cgttcccgcc
     7201 ccatgagagc aaaataccga atctgcagta ttttctacat ctgcatccgc atcgtatccg
     7261 atctgcccga tcacttcttc tgtattatga cattcgccgt atcctttcaa agaacaaaac
     7321 aatcttccgt aaccgcctgt gtacgccgta aattcttttt gatatccatt catctcggcg
     7381 gcaggaacac ttcctttaag caccgccatc ccattttccg tctcaggtcc ttcaaactct
     7441 ccgctcatcc gttttatatc cgtcattgct tttccgacat tttccaacgg cagttccata
     7501 cgaaattcgt agtacggttc aagaagaaca ctttccgcct tccgaagtcc ctgacgtacc
     7561 gcacgatatg tcgcctgcct gaaatcaccg ccttctgtat gctttttatg cgcctttccc
     7621 gacagaagcg taattcggac atctgtaaga gcagaacctg tcagtactcc tttatgttcc
     7681 cgctcttcca aatgcgtaag tatcagcctc tggtaattac gatccaaaac atcctccgaa
     7741 caatctgccg caaactgcat ccccgatccg cgctctcccg gctctaatct caaatgcact
     7801 tccgcataat gtcgaagagg ttcaaaatga cctgcccctt ccacactgtt tttgatcgtc
     7861 tccttgtata caatgcgtcc ttctccgaaa tgaatgagaa ctccgaaccg gtctttcacc
     7921 atcttctgca gaacttccgt ctgcacttct cccataagac ggatgtggat ctctgatgtt
     7981 tcctcgtttc tgatcacatg aagctgcgga tcttcttctt caagacatcg cagattctga
     8041 aacattttat gagcgtcaca cccgtccgga agttcgatcc gatatgtcat gacaggttct
     8101 aatacaggaa gcaaagattc tttctgcgcg ccgatcccct gtcccggata agttccttca
     8161 agtcctgtca ccgcacatac ttcccctgcg gaaactgttt gaacgagttc gtatttttct
     8221 cccgaatact ttcggatctg attgaccttc cctgtacatg attgttctgc cgcaaaagga
     8281 acatctttga cgcgcagctg tcctcctgtg actttcagat aagtcagcct gtttccctga
     8341 ggatcacggg cgatcttata gacttttgcc ccaaattcag gcaaaggctc tgccgacggc
     8401 gcatattcac ataatcccga catcagcgct tcgattcctt gcgctttcaa agcagaacca
     8461 aaccaacacg gaaatacttt ccttgcggca accgcctttt tcacagtctc cttttcgatc
     8521 ttcccgcctg ataaatattc ctcaagcatt gtctcatcac acatcgcaag actttctctg
     8581 ttctcctccg actgcatgtc catctccaca cagccgtctc cgaatcggtt ttttaattct
     8641 tccagcagtt tttctctgtc tgcttcctga cgatccattt tattcacaaa aatcattgtc
     8701 ggaatcttgt actgtttcag cagcctccaa agcgtgaccg tatgtgcctg taccccgtcc
     8761 acagcgctga caacaagtac ggcgcagtca agcacctgca gcactcgttc catctctgct
     8821 gaaaaatcca catgcccggg agtgtcaaga atcgtaatac tcgtatcatt ccatataaat
     8881 tccgcctgtt ttgaaaaaat cgtgatcccg cgttctcgtt ctgtttcata attatcaaga
     8941 aatgtatccc catgatcgac gcgccccata ttccgaatct gtccgcttaa ataaagcatt
     9001 ccttccgaca atgttgtctt gcccgcgtcc acatgggcaa caagaccgat acatatatgt
     9061 tttaatggtt tttttaccga atttttgtct ttcttttcgg atgtattttc catgaaaata
     9121 cccctttcct gtgattttta tacagaaatt atcatatcac agaaaggggt ataagtcgaa
     9181 tgtaaaacgt tttctacttt tccactctat ttttctacag tctttcgctg ctttcattca
     9241 taaatttgta ttctgccacg gaacggtcaa aacctttaat atgtctgtac agccactcga
     9301 taacattttt attcacctgc tctacaaatg cctctgacgg tccttcttcc tcttcaagca
     9361 tattgtaaag atcttttact acggcacgca gtttgtcatg ttctttttta tgttccgcta
     9421 ttgccggata attgatcgat tcctgaagtt tttcttcctc cccgaaatga aactctgtat
     9481 agtctgcaag ataatcaagc gttttcactg ccactacttt ttctttgctc gtctcacaac
     9541 tatcaagaag cttattgatc ttgtcaatca gttctttgtg ctgtgtatcg atcatttcat
     9601 ttcccgttac caaactttca tcaaattctg ctctcatcgt ttcattctcc tttttctttt
     9661 ttcactgccg ttctttcgtt tttaaactgc aggatcattt catttgtaag actatcccgt
     9721 gatggctcta caggaatgtc ttccctttca aaccattctg ccaccgccag ttcctctctg
     9781 tcaagtgtga ttgcatcatt cccgtcaaga tcgcaataaa atcccataag aagcgtatcg
     9841 ctgaacgccc acggctgact tttataatac cggatgtttt tcactttcag cccgacttct
     9901 tccatcactt cccgcgccac agtctgttcg acagtctccc cgatctccga aaatcctgct
     9961 aaaagcgcat attttttata tgtacgtcct gcatatttgg acatcaaaat ccggttgtga
    10021 tccgtcacgc cgacaataac ggccgggcat atcttcggaa attccgtatt tccgcacgct
    10081 tcacagacaa gcatccgctc cttctccccg tgcttcagcc gttttccgca ccgtccgcaa
    10141 tatttccggt ctttatacca attataaagc tgatatcccg taattccggc aaatgcccgg
    10201 tcttgcggat ctgccgttct gaaaatctct gtatttttca tttggaagtc taaaagcatc
    10261 gatctgtcaa tttcatttac gagataataa cgctcatcat ctactgaaaa taaatatata
    10321 cactctttgt aaatctccct gttcaaatgt tccaaatccg caaaactcgg aaaactgatt
    10381 cccttttcag acactttcat cagcgccgcg tgatcttcat aatacaatgc aatactgtct
    10441 ttatcaggcg gaaccggttt gtactgatta tctaagtgat gcggttcaat gtcttgtatc
    10501 atagcagttc ctttcccata cgccgcagtt tttctttcct tttgcagcga cgctataagt
    10561 ttctgatctt aaattctctc tgtgcttccc gtttttgatc ttttttagca atatcgtccc
    10621 gcttatcata cagtttcttt ccttttgcca ctccgatctc cattttcaca agactcccct
    10681 taaaatacac ctgcagcgga acaagagtaa ttccctgttc tttcatcttt ccgaacatct
    10741 tgtctatctc ttttttatgg agaaggagct ttctcaccct gagcggatct ttattaaata
    10801 tgttcccttt ctcatacgga gagatgtgca tcccgtaaat atacatttct cctgactcca
    10861 ccctgataaa agcttctttt atactgcatt ttcccattct cagagatttt acctctgtac
    10921 cgtgcagaac aattcctgct tcatattttt caagaataaa atagtcatga tacgctttct
    10981 tattattggc gatcatcctg acttctgact ttgccataat ccactcttct cctgccctaa
    11041 aatctatcat gtccaaatac tttcacatac ccgaacacct ctttgtacga atgctttaca
    11101 tacgcaaata tctctttctt acagaatcct gaaattcact gtcctttgaa gtttatccgc
    11161 atctgtgacg caaattctca ccttctgtcc cggcttataa gtcttgcctg tatgttctcc
    11221 gaccagttca tagctttgtt cataaaactc ataatgatca tcccacatat ccgccacatg
    11281 tgcaagtcct tccaccgtat tttccaattc tacataagcg ccccactttg tcactcccga
    11341 aatcactccg tcaaactctt ccccgatatg atctctcata tactcagcct ttttcagctt
    11401 cacaacttcc cgctcggctt cttcggctct tctctccctt tcactgcact gtgccgcaac
    11461 ctgctgcaaa agttcagaat aatgctccgc ccggttctca tcaaatcttc ctcttaagtt
    11521 ttctttgatg atacgatgga tctgaagatc gggatacctt cggatgggcg atgtgaaatg
    11581 agtataatat tttgccgcaa gtccgaaatg tccggtattc tccgtcgtat acctcgcctg
    11641 tttcatcgac ctgagcgtaa gtctgctgat cagatcttcc tccggttttc cctctacttt
    11701 ctcaagcagt ttctggatct ctttcggccg gatctcattt ccgacatgga tatgatagcc
    11761 gaagctgttg acaaatgccg aaagctgtct gatccggtcc tcatccggcg cttcatgcgt
    11821 ccggtaaaga aacggaatct ccctccagaa aaactcttcc gccactgtct catttgccat
    11881 aagcataaaa tcttcgataa tctctgtcgc cggatttctg tcatacggct ttacttcgat
    11941 cggttttcca tcttcatcca aaacaatctt cgtctccgga aagtcaaaat cgatagcgcc
    12001 ccgttttccc cgtttctgtc tgatcaactc cgataattct ttcatcatcc atatcatcgg
    12061 aacgaatcct tcatactcac gaagcgcctg ctcatcctgc tctaatacag cggaaacttt
    12121 gttgtaactc atcctcttgt caacacagat caccgactct gtaatctcat gggaaatcat
    12181 ctcccctttc ggcgagatcg tcatgataca ggacaaagca agacgctctt cccccgcatt
    12241 gagcgaacag accccgtttg acaaaatgtg cggaagcatc ggaataaccc tgtcagccaa
    12301 atagacgctt gttccgcgtt ccaacgcttc tctgtcgagg gcgctttttt cctgaacata
    12361 attcgttaca tccgctatgt gtacgccgag cacataattt tctccgtccc gctctaccga
    12421 tacggcatca tccagatctt ttgcatcttc actgtcgatc gtcaccattt tcacattcgt
    12481 aaggtctttt ctcccggcac gatccgcctc acttacaggc ttcccgactc tgaccgcctg
    12541 attcaaaact ttctcgggaa actctgtctt aatgccaaga tccattacga ttgacaaaat
    12601 atccgtgccc ggatcgttca catgtccgat gatctgcgta acttttcctt ccggtttcat
    12661 atgttccccg ccgtaagacg tcatttccac aacaacttta tgacctgtca cagcgcccat
    12721 ctcacgccct gacggaatat aaatatcgtt tacataccga cagttatccg gcctgacaaa
    12781 cccgaaactt ttacattttt catacaatcc gacaatatgc gttactccgt gcgaaataat
    12841 tccgacgatc tttccctccc gccgctttcc tgccggagct gacgttataa tatactctac
    12901 ttcatcgcct tggaatgcat tcccaagaca ctcttccgga ataaagatgt catctgttcc
    12961 atcttccggc gtaacaaaac cgaaacctct tgcatttgcc tgaaatattc ctttcagacg
    13021 ctcggtatga ccgcatgtat attttcccct ttttgaaatc gtgatcttcc cttcttccac
    13081 aagagcatcg agaacttccc gcagttcctg tctctgttct cccggaattt gaagtacgac
    13141 ggcaatctct ttgatcttca taggtacgta cagatcatcg ttgataaaat caaaaagtac
    13201 ctttttgcgt ttttcaaatt gacggctttc ctcttttgat tttgaaggat tttttctgtc
    13261 attcactttg acgcgatcat catgtattct tctttttttc tctctgttac cgtgtgtttt
    13321 ccgcatttta ccctccgcac taaaataatc gccgaaagta ccaaaaggca cattcccgat
    13381 tgtaaacagc cgccagattc tcagacgtct ccgagctccg gctcttcgcc gttacaaagc
    13441 aaaagcaccc tatattcgta tagagtgctc ataatcatta ttatcttctg ctgtcttttt
    13501 atactatcct tttagttaaa gatattgaga ttcagtacaa ctgccaatac aataaacaga
    13561 accgcaataa ctcttgtagc ctttacaagc gcgccttcca tcgaacgtcc tttattatgt
    13621 ccccaatatg tatctgcagc tccgctgatc gttccaagac ctgcggattt accctcctgt
    13681 aaaagaataa tacaagctaa tgcgatgcaa tctataacaa aaataatcgt caatactgtt
    13741 tttaaaatat ccatttcttc tacctcctga aataaaccaa aattattcta tcacaattac
    13801 agcttattcg caagacattt catccattgt tttctcaaac cttgttttct caaatattca
    13861 ttcattgtgt cacaaaatat attatgttac aatcatctta aaattataat ttttttat
//
LOCUS       DBMDXF010000029        13797 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1295_length_13797_cov_2.206703, whole genome shotgun sequence.
ACCESSION   DBMDXF010000029 DBMDXF010000000
VERSION     DBMDXF010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1295_length_13797_cov_2.206703"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(405..2048)
                     /locus_tag="ACZPD4_02265"
     CDS             complement(405..2048)
                     /locus_tag="ACZPD4_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1377936.1"
                     /translation="MNYASIIIPFVGGLGMFIYGMQIMAQGLENAAGSRMKSLLEALT
                     KNKLFGVMLGAFITAVIQSSSATTVMVVGFVNAGIMNLSQAMGVIMGANIGTTVTGWL
                     VSSVEWAKFLSPANLAPIAIIIGVIIMLTGKRRSSKDISSIIVGFGILFVGITTMSSA
                     VAPLQESEAFCSIFVKLGHNPFLGILAGALVTAIIQSSSASVGILQSLAAAGLVPFNA
                     AVYIIMGQNIGTCVTAIMSSIGAKKTAKTAAIMHLLFNVIGTIIFSVAAIFFFKVLNP
                     GMGAGMITQTEISTVHTIFNIGTTLLLFPVSDWIIKLARKLQKEEDGVVDESRVLLDE
                     RMLETPSIAIQSVVSEVCRMGYIVKGTMDKTRRVLFTQDEETMNEIREEESIADRLCK
                     GITDYLIRISSLAINEKEHQHVASLLQIVSDIERISDYCENISEYAENLANQKKKFSG
                     VGATEMKEMLEVCSDSFTYAVEAFEENSAEKALKVIEKERSADDLEIRLRADHMQRLA
                     NKQCDTEAGIVFLDALVGLERISDHSRNIAEEVLSASNK"
     gene            2267..3076
                     /locus_tag="ACZPD4_02270"
     CDS             2267..3076
                     /locus_tag="ACZPD4_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377937.1"
                     /translation="MMSIKHYTDPELLEINTLENAIYMAMQNDVSFMIGLRLSLYEHQ
                     STFNPNLPLRYLFYISDLYSGMIDKNKLYGSRKILIPPEFVIFYNGIQDQPDRRILRL
                     SDLYSIRDQHCKLELEAVMLNINLGHNPALMNACTMLHQYAEYTDRVRQYTKTMSLED
                     AVEHAITECIHEGILKSFLEKNRAEVKNVCIYEYDEEEHIRLERSYAWEDGKKEGLKE
                     GIRKGEKGKTCNLIQKKLEKGKTLSQIADELEDTEENIGRLIEQFHLGIPQ"
     gene            complement(3188..3544)
                     /locus_tag="ACZPD4_02275"
     CDS             complement(3188..3544)
                     /locus_tag="ACZPD4_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1377938.1"
                     /translation="MDYVKAFIVGGLICAAVQILLDRTKLMPGRVMVLLVCGGALLGF
                     CGLYDPFQKLAGAGAGVPLPGFGNVLWQGVKEAVDKYGFMGVFMGGFQASAVGISAAL
                     IFGYIASFIFEPKMKK"
     gene            complement(3635..4681)
                     /locus_tag="ACZPD4_02280"
     CDS             complement(3635..4681)
                     /locus_tag="ACZPD4_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1377939.1"
                     /translation="MDQIKGAQSIMFREAPYLISAASVAGSKEADGPIGKLFDVTDKD
                     DLFGAQTWEEAESNMQKEACVLAAGKAHVDLKKIRYLFGGDLLRQGIATSMGVEALQI
                     PMFGLYGACSTSGEALALASMSAAAGYGGTMIAVTSSHFGSAEKEFRFPLGYANQRPL
                     SSHWTVTGSGAFLVQSAEEYRKQNTKSYFSNIRITGVTVGKIVDYGLKDSQNMGACMA
                     PAAMDTIVQNFQDMERKEKDYDRIITGDLGSIGRTILFDLIREKGYDICEKHMDCGMT
                     IFDKERQDTHAGGSGCGCAAVTLASYVLPKLEKGEWKRVLFVPTGALMSTVSFNEGAS
                     VPGIAHGIVLESDA"
     gene            4915..5376
                     /gene="spoVAC"
                     /locus_tag="ACZPD4_02285"
     CDS             4915..5376
                     /gene="spoVAC"
                     /locus_tag="ACZPD4_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HIR1377940.1"
                     /translation="MDKIQKLKQEKQYEQYVKEKTPRHNLFLNMCKAFFLGGLICLLG
                     QVIYHYFELRNLSPDECSRWMSLSLILLSVLFTGTGIYQKLAAWGGAGALVPITGFAN
                     SVAAPAIEYKKEGQVFGIGCKIFTIAGPVILYGIFTSWGLGLIYWFGTLFK"
     gene            complement(5369..6856)
                     /gene="malQ"
                     /locus_tag="ACZPD4_02290"
     CDS             complement(5369..6856)
                     /gene="malQ"
                     /locus_tag="ACZPD4_02290"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1377941.1"
                     /translation="MERASGVLLPIFSLPSKYGIGCFSKEAYQFIDRLKAAGQSYWQI
                     LPLGPTGYGDSPYQSFSTFAGNPYFIDLETLTGEGLLTAQECEYGCERIREDKIDYEK
                     IYKIRFQILEKAFSRFGENDEYRAFVSENQFWLEDYSLYMAIKDRNGGVSWNEWEEPL
                     KNKESQAIENAKAELSRQIAFYKFQQYEFDRQWKRLRSYANENGIEIIGDIPIYVAFD
                     SADTWSAPEMFRFNDALEPIDVAGCPPDGFSQTGQLWGNPLYDWEYHKKTGYDWWIRR
                     IEHCFRLYDVVRIDHFRGFDEYYAVPYGEKTAVHGKWMPGPGIELFRTLEEKIGRKRI
                     IAEDLGFLTPSVIQLLKESGFPGMKVLQFAFDSREDSNYLPHTYTRNCVVYTGTHDND
                     TTKGWYHTAAGSTRQFAKEYMYKPRLDEDTLAGDFIAMAMGSAADLCIVPMQDYLGLG
                     SDARINTPSTLGGNWEWRMKPGEPDEGTVREMERMTKIYGRLRFT"
     gene            complement(6909..9176)
                     /locus_tag="ACZPD4_02295"
     CDS             complement(6909..9176)
                     /locus_tag="ACZPD4_02295"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1377942.1"
                     /translation="MRTGIQEELELYMSKSVAQASNREIYEALLLFVQKLAAGKERAA
                     GKKKLYYISAEFLIGKLLSNNMINLGIYDEVKEMLFQNGKDICQIEEVEPEPSLGNGG
                     LGRLAACFLDSIATLGLPGDGIGLNYHLGLFKQEFKDHLQKELPNPWIEKQSWLTKTD
                     VVYPVSFRGLKVNARMYDIAVTGYHDQTNKLHLFDIDSVDEGIVEDGIHFDKEDIAKN
                     LTLFLYPDDSDEKGRLLRIYQQYFMVSSAAQMILDECVQKGSTLYDLPDYAVIQINDT
                     HPTMVIPELIRLLVERGLDIDEAIDVVSRTCAYTNHTILAEALEKWPIGYLKKVVPQL
                     VPIIEILDDKVRRRFSDESTAIIDRNDTVHMAHIDIHYGFSVNGVAALHTDILKQSEL
                     NHFYKIYPDKFNNKTNGITFRRWLLHCNPRLTALLDETIKDEYKEDASKLVKLLEYKD
                     DETVLKCLLEIKKENKKDLAAYLKETQAIEIDADTIFDIQVKRLHEYKRQQLNALYII
                     DKYLEIKEGILPKTPITAIFGAKAAPAYVIAKDIIHLILCLQEIINNDPEVSPYLKVV
                     MVENYNVTKAEKLIPACDISEQISLASKEASGTGNMKFMLNGAVTLGTEDGANVEIHE
                     LVGDDNIFVFGASSDEVIEHYKKADYSAKDYYKKNPKIKAAVDFITEKQMMKIGCKES
                     LERLQKELIGKDWFMTFLDFDAYKKAKDKALAAYTDQKEWAKKMLVNIAEAGYFSSDR
                     TIEQYNRDIWKLTRS"
     gene            complement(9230..10246)
                     /locus_tag="ACZPD4_02300"
     CDS             complement(9230..10246)
                     /locus_tag="ACZPD4_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1377943.1"
                     /translation="MKLIKTEEAVGSVLCHDITQIIPGVVKDAVFRKGHVVTQEDIPV
                     LLSVGKEHLYVWEKQEGMLHENEAAEVLRRVCQGRFLKASKPKEGKIELTAETDGLLK
                     VDKEKLNAVNGLGQIVIASRHGNFPVKKGDKVAGMRVVPLVIEEEKMEQVNRMCGNEP
                     IFTILPFCQMKAAVVTTGSEVYHGRIEDKFTPVVKEKLRECGMEVIGDTLCSDDADMV
                     TEAVKKWIEQGAELVVCTGGMSVDPDDRTPLAIRNAADEVITYGAPVLPGAMFMLAYA
                     GKVPIMGLPGCVMYAKRTIFDLILPRIAAGERLKAEDFTALGEGGLCLNCPVCTFPNC
                     SFGK"
     gene            complement(10377..12296)
                     /locus_tag="ACZPD4_02305"
     CDS             complement(10377..12296)
                     /locus_tag="ACZPD4_02305"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1377944.1"
                     /translation="MRHGFVKVAAATPDIRVADVEYNTEQICRAIDQAYEKGAKIVAF
                     PELCVTGYTCGDLFTQNVLLNAAYGAVKKIAGHTKGKNILAFVGMPLTEGGKLYNTAA
                     AVNDGKVIGFTTKTFLPNYGEFYEMRQFTPGPDSVRWIQFDGEKVPFGPKILFQDDET
                     ADLIVGAEICEDVWSPVPPSIEEALAGATIIVNCSASDETVGKGSYRTRLIAGQSARL
                     IAGYVYANAGEGESTTDLVFGGHNIIAEDGTILRQSERYKNEIIYSEIDLEKIVGERR
                     KNTTFSPEKLNCGCPVVVPFHVGICDTVLTRNFSQHPFVPSDEAARAHICDEILTIQA
                     MGLKKRLSHTNAKSAVVGISGGLDSTLALLVTAKAFDMLGRDKKEIIAVTMPCFGTTD
                     RTYNNACEMTRQTGATLIEVPIGEAVNVHFRDIGHDPDVHDVTYENAQARERTQVLMD
                     IANREAGMVIGTGDMSELALGWATYNGDHMSMYGVNASVPKTLVRHLVQHAADTTENE
                     SLRQVLYDVLDTPVSPELLPPKDGDIAQKTEDLVGPYELHDFYLYYMLRFGFSPEKIF
                     TLAVNAFAGVYEKETILKWLKIFYRRFFSQQFKRSCLPDGPKVGSVALSPRGDWRMPS
                     DACAALWLKELEQLQ"
     gene            complement(12411..13286)
                     /gene="hslO"
                     /locus_tag="ACZPD4_02310"
     CDS             complement(12411..13286)
                     /gene="hslO"
                     /locus_tag="ACZPD4_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1377945.1"
                     /translation="MKDYIVRASAANAQIRAFAAVTTELTEEARKRHETSPVATAALG
                     RLLTGGAMMGSMMKNDTDMLTIQIKCSGPIGGLTVTADSKGNVKGYVHEPNVILPPKN
                     GKLDVGGALGQGVMTVIKDMGLKEPYSGQTILQTGEIAEDLTYYFATSEQVPSSVGLG
                     VLMEKDNTVRCAGGFIVQVMPFIEDEVLNKLEENIQNIRSVTSMLDHGHTPEEMLAQV
                     LEGLDLEITDTMPAKFYCNCSKKRIEKAIISIGKKDIQSMIDDGKDIEVKCHFCNEAY
                     NYTVDELKELLKKSR"
     gene            complement(13401..>13797)
                     /locus_tag="ACZPD4_02315"
     CDS             complement(13401..>13797)
                     /locus_tag="ACZPD4_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1377946.1"
                     /translation="GVCSFIWDNRYYEKEQINEYDLTLFIAEDFNPMENAYVSERTAD
                     SEDALLSEEGAGDLEDTMFSEEEGGENGSLYRRYTETHYQRGYTLAEIQELLERAGLV
                     FIEAYDADTKETPNDTSERICVIARENGK"
BASE COUNT         3472 a         3616 c         2497 g         4212 t
ORIGIN      
        1 atctctctgt tttcttttcc tgtcataggc ttctcctttc atgcgcatga acgagtccta
       61 tgaactaaga gaaaagtttt cacaataaac tttattttct ttcttaagaa tcagtatacg
      121 aaaaaaagcg gtgctctaca agactctcca tgcaaaaata attttgttaa tcgggtaact
      181 ttgagggaat tcctcggact gacgatactg tcagcattct ccgcggacag acgagactgc
      241 cggcgctttt cacagaaaga atttctgtga gtggatttat cataacatat ttacaaatca
      301 ggaacaatga tattttatta caaggatctg cactctaacg tcagtatacg ataaagaata
      361 cggctgcacc gcagctttca ctgccatgca gccggaataa gtttttattt attacttgcc
      421 gaaagcactt cttccgcaat atttcttgag tgatcggaaa tcctctctaa gccgacaagc
      481 gcgtcaagaa atacgattcc cgcctccgta tcgcactgct tatttgcaag acgctgcata
      541 tgatctgccc gaaggcggat ctccagatca tcggcgcttc tttctttttc aatcactttc
      601 agcgccttct ccgcactgtt ctcttcaaat gcttcaaccg cataagtgaa actgtctgaa
      661 cagacttcca gcatctcttt catttctgtt gccccgacac ccgagaactt ctttttctgg
      721 ttcgcaaggt tttctgcata ctcggaaata ttttcacaat agtcgctgat tctttcgatg
      781 tctgaaacaa tctgaagaag gcttgccacg tgctgatgct ctttttcatt aatcgccaga
      841 gagctgatcc gtatcagata atctgtaatt cccttgcaga gccggtccgc gatactttct
      901 tcctctcgga tctcgttcat cgtctcttca tcctgagtaa ataatactcg gcgagtcttg
      961 tccatcgttc cttttacaat atatcccatc cggcatactt cgcttacaac agactgaatc
     1021 gcaatactcg gagtctctaa catcctttca tcaaggagca cacggctttc atctactact
     1081 ccgtcttcct ctttctggag ttttcttgcc agtttgatga tccagtcgga taccgggaag
     1141 agcagcagtg tcgttccgat gttgaaaatt gtatgtaccg tactgatctc tgtctgcgta
     1201 atcatacccg cgcccatacc cgggttgagc actttgaaga aaaagatcgc cgcaacactg
     1261 aatatgattg ttccgatcac attgaacaaa agatgcatga tcgccgctgt ctttgctgtc
     1321 tttttcgctc cgatacttga catgatcgcc gtcacacatg ttccgatatt ctgccccatg
     1381 ataatatata ccgccgcatt aaacggtaca agtcctgctg ccgcgagact ctgcaagatg
     1441 ccgaccgatg cagacgaact ctggatgatc gccgtcacaa gcgcacctgc aagaatacca
     1501 aggaacggat tgtgtccaag cttcacgaaa atgctgcaga atgcttctga ttcctgaagc
     1561 ggcgcaaccg ccgacgacat cgttgtaatt ccgacgaaaa ggataccgaa ccctacgata
     1621 atgctggaaa tatcttttga tgaacgtctc ttgcccgtca gcatgatgat cacaccgata
     1681 ataatcgcaa tcggcgcgag attggcagga cttaaaaact tcgcccattc cacactcgac
     1741 acgagccagc ccgttaccgt cgttccgata tttgctccca tgatgacgcc catcgcctga
     1801 gacagattca tgatacctgc gtttacaaac cctacgacca taactgtcgt tgccgacgaa
     1861 ctctggatca ctgccgtaat aaatgcgccg agcatcactc caaaaagttt atttttcgtc
     1921 agcgcttcca atagagactt catcctgctg cccgcggcat tctccaaacc ttgcgccatg
     1981 atctgcattc cgtagataaa cattcccaag ccacccacaa acgggatgat aatagatgca
     2041 tagttcataa ttctactctt tcctccgtag ctataagagt tttttcattc acttttttca
     2101 cataatttat gcagagctgc aaaagtgtgc aaccctgcat tcttacgata ccattatcag
     2161 cgtcaaaaaa caacaaaatc tttgcatttt cttagcgcaa ttttcacaga cgttttgtgg
     2221 atgtaatata cacataaaaa actggcgttt tctacacttt catgctatga tgtcgataaa
     2281 gcattatacc gatcccgaac tgttggaaat caacacctta gaaaacgcga tctatatggc
     2341 aatgcagaac gatgtttcat tcatgatcgg acttcggctg tctttatacg aacatcagtc
     2401 aacttttaat cctaatttac cgctgagata tctgttttat atttcggact tatactcggg
     2461 tatgatcgac aaaaacaaat tatacggttc acgaaaaatt ttgattccgc cggaattcgt
     2521 catcttttat aatgggatcc aggatcagcc ggaccgccgg atcttacgat tatccgattt
     2581 atattccata cgggatcaac actgcaaatt agaattagaa gctgtcatgc tcaacattaa
     2641 tctcggacat aatccagcct taatgaatgc atgtacgatg ctgcatcaat acgcagagta
     2701 tacagaccgg gttcgtcagt acacaaaaac aatgtcgctt gaagatgcgg ttgaacatgc
     2761 catcacagaa tgtatccacg aagggatttt aaaatcattt cttgagaaaa atcgagcgga
     2821 ggtgaaaaac gtgtgcattt acgaatatga cgaagaagaa catatccggc tggaacgcag
     2881 ttacgcctgg gaagacggaa aaaaggaagg acttaaagaa ggtattcgga agggcgaaaa
     2941 agggaaaact tgcaatctca ttcagaaaaa actcgagaaa ggaaaaactc tttcccagat
     3001 tgcagacgag cttgaagata cggaagaaaa tatcggacgc ctgatcgaac agtttcatct
     3061 tggaataccc caataaccga agcgacttat atagagaaaa acaattataa ttctctgcag
     3121 acacagtgtg tacagaaatc ttctttattt tatttttctt gatttctgta cacattttca
     3181 taaaaatcta ttttttcatt ttcggttcaa agataaagga cgcaatataa ccgaagatca
     3241 gcgctgcgga aataccgacg gcactcgcct gaaatccacc catgaacaca cccataaatc
     3301 catatttgtc aacagcttct ttcacgccct gccaaagcac attgccgaat cccggaaggg
     3361 gaacgcctgc ccctgccccg gcaagtttct gaaacggatc gtacagtccg caaaatccaa
     3421 gcaaagctcc gccacatact aaaagcacca taacccttcc cggcatcagc tttgtcctgt
     3481 ctaataaaat ctgcacagcc gcgcatataa gacctcctac gataaacgct tttacataat
     3541 ccattttgtt tcaccctttc tcctttctac tctgctcatt cttcttgctt ttatcatttt
     3601 gccttctatc ttcttgcttt ttaatttcct tatgctatgc atcgctttcc agaacaattc
     3661 cgtgagcgat ccccggtacg ctcgccccct cgttgaaact gacggtagac ataagcgccc
     3721 ctgtaggaac aaacaacacc cgcttccatt ctcctttttc taatttagga agtacatacg
     3781 atgccagcgt tacggcggcg cagccgcatc cgcttccacc ggcatgcgta tcctgccgtt
     3841 ctttatcaaa aatcgtcatt ccgcaatcca tatgtttttc gcatatatcg tacccttttt
     3901 ctctgataag atcgaataaa atcgttcttc cgatgcttcc taaatctcct gtaataatcc
     3961 tgtcgtaatc tttttctttt ctttccatat cctgaaagtt ctgtacaatc gtatccatgg
     4021 ccgccggagc catacacgcg cccatatttt gagaatcctt caagccataa tctacgatct
     4081 ttccgacagt tactcctgta atgcgaatat tagaaaagta cgactttgta ttttgttttc
     4141 tgtattcctc tgccgactgc acaagaaacg cgccgcttcc cgtcactgtc caatgtgaag
     4201 aaagaggtct ctgattcgca tatcctaacg gaaagcgaaa ctctttttcc gcacttccaa
     4261 aatgacttga tgtgacggcg atcatcgttc ctccgtatcc tgcggcagcg ctcatagatg
     4321 caagcgcaag cgcttctcct gatgtagaac atgctccgta gaggccgaac atcggaatct
     4381 gcaaagcctc tacccccatt gaagttgcta ttccctgccg caaaagatcc cctccaaaaa
     4441 gatatcggat ctttttcaag tccacatgtg cctttcctgc cgcaagaaca cacgcttctt
     4501 tctgcatgtt actctccgct tcttcccatg tctgtgctcc gaacaggtcg tctttatctg
     4561 tcacgtcaaa taatttcccg attggtccat ccgcctcttt gctgcctgcg acagacgcag
     4621 cgctgataag atacggcgct tcccgaaaca taatgctttg cgcccctttt atctgatcca
     4681 taatccttcc tttccctgac tccgtcttcc atcagccacg ttacgcacgc attgcgtttt
     4741 acacatggcg tgggctgaac tgttacagtt ttatttttat tatttttatt gtcggcatat
     4801 ttgcgaaaaa ttattatgaa tttatataca tattcggaaa attatttcag atactaacag
     4861 aaaagaaatt aaaaacgcgc gtgttgcgcg aaatgcaagg agtgtgtttc atctatggat
     4921 aaaatacaaa aactgaaaca agaaaaacaa tatgaacaat atgtaaaaga aaaaacgccc
     4981 cgacataatc tttttctgaa tatgtgcaaa gcgtttttcc tcggcgggct gatctgcctg
     5041 ctcggacaag ttatttatca ttattttgaa ttacgaaatc tttctcccga tgagtgcagc
     5101 cgctggatgt ctctgtcgct catcctttta agcgttttgt tcaccggaac cggaatttac
     5161 cagaaacttg cagcctgggg aggcgccgga gcgctcgtgc cgatcaccgg attcgcaaat
     5221 tcagtagctg cccctgccat cgaatacaaa aaagaaggtc aggtattcgg catcggatgt
     5281 aaaattttta cgatcgccgg tcctgttatc ctgtacggta ttttcacaag ctggggactg
     5341 ggactgatct actggttcgg aactttattt aagtaaaacg cagccgtccg tatatttttg
     5401 tcattcgctc catctcccga actgtccctt catccggctc tcccggtttc atacgccatt
     5461 cccaattccc gccaagtgtg gacggcgtat taattcgtgc atcgcttcca agtcccagat
     5521 aatcctgcat cggaacgatg cacagatccg ccgcgcttcc cattgccata gcaataaaat
     5581 ctcccgcaag cgtatcttca tcaagacggg gcttatacat gtattctttt gcaaactgcc
     5641 gtgttgatcc tgccgccgtg tgataccatc cttttgtcgt atcattatcg tgagttcccg
     5701 tatatacaac gcagtttctt gtatatgtat gcgggagata gtttgaatct tcccttgaat
     5761 cgaatgcaaa ctgaagcact ttcatccccg gaaatccact ttcttttaaa agctgtatca
     5821 cactcggagt taaaaagccg agatcttcgg caataatcct ttttctgccg atcttttctt
     5881 ccagcgtccg gaacagctct atccccggtc ccggcatcca ctttccgtgt actgccgttt
     5941 tttctccgta cggaacagca taatattcat caaaaccgcg gaaatgatcg atccgcacga
     6001 catcatacag ccggaaacag tgttcgatcc tgcgaatcca ccagtcgtac cctgtttttt
     6061 tatgatattc ccaatcgtag agcggattcc cccacaactg tcctgtctgc gaaaatccat
     6121 ccggcggaca tcccgcaaca tcgatcggtt caagagcgtc attaaaacgg aacatctccg
     6181 gcgccgacca tgtatccgcg ctgtcaaacg caacatagat cggaatatct ccgataatct
     6241 ctatgccgtt ttcgttcgca taagagcgaa ggcgtttcca ctgtctgtca aattcatatt
     6301 gctgaaattt gtaaaaggca atctgacgcg acagctctgc ttttgcattt tcaatcgcct
     6361 gtgactcttt gtttttaagc ggttcttccc attcattcca actcacgccg ccgtttcggt
     6421 ctttgatcgc catatagagg ctataatctt ccagccaaaa ttgattttcc gaaacaaatg
     6481 cgcggtattc gtcattttct ccaaaacgcg aaaacgcttt ctcaagtatt tgaaaccgta
     6541 tcttatatat tttttcataa tctatcttat cttcccgtat tctctcacat ccgtattcgc
     6601 actcctgcgc tgtcaacaaa ccttctcctg taagcgtttc aagatcgata aaatacggat
     6661 tgcctgcaaa tgtagaaaaa gactgatacg gagaatctcc gtatccggtc ggccctaacg
     6721 gaagaatctg ccagtaactt tgccctgccg ccttaagcct gtcaataaat tgataggctt
     6781 cttttgaaaa acatccgatt ccatattttg acggcagtga aaatatcgga agcagcacgc
     6841 cgcttgcccg ttccataacc atcccccttt tcatcaatat accgttttac taatattttt
     6901 ctttaatctt atgatctggt taatttccaa atatctctat tatactgttc aatcgttctg
     6961 tctgatgaga agtagcctgc ttcggcaata ttgacaagca tctttttcgc ccattccttt
     7021 tgatctgtat acgccgccag cgccttgtct tttgcctttt tgtacgcatc aaaatcaaga
     7081 aatgtcataa accagtcttt tcctatcagc tctttttgca gacgctccag actttctttg
     7141 catccgatct tcatcatctg tttttctgta atgaaatcaa ccgccgcttt gatcttcgga
     7201 ttctttttat aataatcttt tgccgaataa tccgcttttt tataatgttc gattacttcg
     7261 tcgcttgacg ctccgaacac gaaaatatta tcgtctccta caagttcatg tatctctacg
     7321 tttgccccgt cctctgtacc gagagtgact gcaccgttta acataaactt catattcccc
     7381 gtgccgcttg cctcttttga cgcaagagag atctgctcgg atatatcaca tgcaggaatc
     7441 agtttttcag cctttgtcac attataattc tctaccatga caactttcag ataaggactt
     7501 acttccggat cattattaat gatctcctgc aggcaaagta taagatgaat gatatccttt
     7561 gcgatcacat atgccggagc cgctttcgcc ccgaagatcg ctgtgatcgg agttttcgga
     7621 agaatcccct ccttgatctc caaatattta tcaataatat acagtgcatt taactgctgc
     7681 cgtttatact cgtgaagtct ttttacctga atatcgaaaa tcgtatctgc gtctatctct
     7741 attgcctgtg tctctttcag atacgcggca agatcttttt tattttcttt tttaatctct
     7801 aaaaggcatt tcaaaacagt ctcgtcgtct ttgtattcaa gcagtttgac aagtttagag
     7861 gcatcttctt tatactcgtc tttgattgtt tcatcaagca gcgccgtaag acgcgggttg
     7921 cagtgaagaa gccatctgcg gaacgtaatc ccgtttgttt tattattgaa tttatcagga
     7981 tagattttat aaaaatgatt cagttccgat tgttttaaga tgtccgtatg aagcgccgcc
     8041 actccgttaa cgctgaatcc gtaatgaatg tcgatatgcg ccatatgaac agtatcgttt
     8101 ctgtctatga tggctgttga ttcatcgctg aatcttctgc gaactttatc atcaagtatc
     8161 tcaatgatcg gaacgagctg cggcacaacc ttttttaaat atccgatcgg ccatttttca
     8221 agcgcttctg ccaaaatcgt atgattcgta tacgcgcatg tacgggatac gacatcgatc
     8281 gcttcatcga tatcaagccc tctttccaca agaagccgga tcagttcggg aatgaccatt
     8341 gtaggatgag tgtcgttgat ctggatgaca gcataatccg gcagatcata caatgtactt
     8401 cctttttgca cacattcatc caagatcatc tgtgcggcgc tgcttaccat gaaatactgc
     8461 tgataaatgc gcagaagcct tcctttttca tcgctgtcat caggatataa aaacaacgtc
     8521 agattttttg cgatatcttc tttatcaaaa tgaattccgt cttccacgat tccctcatca
     8581 acagaatcga tgtcaaaaag gtgcagtttg tttgtctgat catgatatcc cgtcactgcg
     8641 atatcgtaca ttcttgcatt aactttcaat cctctgaaag aaaccggata gacaacatcc
     8701 gtttttgtca gccatgactg tttctcaatc cacggattcg gaagctcttt ttgaagatgg
     8761 tctttaaact cctgtttgaa caggccgagg tgataattca gtccgatccc gtctccgggc
     8821 agtccgagtg ttgcgatcga atcaagaaaa catgccgcca gtctcccaag ccctccgttt
     8881 ccaagagatg gctccggctc aacctcctcg atctgacata tatcttttcc gttttgaaag
     8941 agcatttctt ttacttcatc ataaataccg agattgatca tattgtttga caacagtttt
     9001 ccgatcaaaa actctgccga aatgtaataa agcttctttt tccctgcagc tctttctttt
     9061 cctgccgcca gtttctgcac aaacaaaagc aacgcttcat aaatctctct gtttgatgcc
     9121 tgcgccacag atttactcat atacaattct aattcttcct gtataccggt tctcatttta
     9181 ctacctccga aaattttcct ctcattcctt ttatgattta tttttcttcc tattttccga
     9241 aactgcagtt cgggaaagta cacaccggac agttcaaaca caaaccgcct tctccgagcg
     9301 cggtaaaatc ttctgctttc agccgttccc ccgccgcaat tctcggcagt atcagatcga
     9361 atatcgtccg ttttgcatac attacgcatc ccgggagtcc catgatcgga acttttcctg
     9421 catatgcgag cataaacatc gcgccgggta aaaccggagc tccataagtg atcacttcat
     9481 ctgccgcatt tcggattgca agaggtgtac gatcatcagg atcaacgctc attcctccgg
     9541 tgcagacaac cagttccgcc ccctgttcga tccacttttt cacagcttct gtaaccatat
     9601 cggcatcgtc actgcaaaga gtatcgccga ttacttccat accgcactct cttagttttt
     9661 cttttacaac aggagtaaat ttatcttcga tccttccgtg atacacttcg cttccggttg
     9721 tcacgaccgc cgctttcatc tgacaaaacg gcagaatcgt aaaaatcggt tcgtttccgc
     9781 acatccggtt aacctgttcc atcttttctt cttcgatcac gagaggtacg acacgcattc
     9841 ctgcgacctt gtctcctttt ttcacaggaa aattcccgtg gcgtgatgcg atcacgattt
     9901 gtcctaaccc atttaccgcg ttcagttttt ctttatcaac tttaagcagt ccgtctgttt
     9961 ccgccgtcag ctcaatcttc ccttctttcg gctttgacgc tttcagaaat ctcccctgac
    10021 atactcgtct taaaacttcc gccgcctcgt tttcatgaag cattccttct tgtttctccc
    10081 atacatacag atgctctttt ccaaccgaga gcagtaccgg aatatcctct tgcgttacca
    10141 catgcccttt tcggaaaact gcgtctttta caacgccggg aatgatttgt gtaatgtcat
    10201 ggcagagtac acttccgacc gcttcttctg tcttgatcag tttcatatat tctcacactt
    10261 tcccactcat tttatataac agttccgtca acacctcgtt tcccaaagag atgttccgat
    10321 gattcctgtt gtttctatct atccttgttg tttccggtta ttcctgttat ttacagttat
    10381 tgcagttgtt ccaactcttt cagccataac gccgcgcatg cgtcgcttgg cattctccag
    10441 tcgcctcgcg gcgaaagagc tacgcttccg actttcggtc cgtccggcag acaagaacgt
    10501 ttgaactgct gcgagaaaaa tctacgataa aatattttca gccatttcaa aatcgtctct
    10561 ttttcataaa cgcctgcaaa cgcattcacc gccaaagtaa aaattttctc cggtgagaaa
    10621 ccgaagcgga gcatataata aaggtaaaag tcatgcagtt cgtacggtcc gacaagatct
    10681 tctgttttct gtgctatgtc tccgtctttc ggcggaagaa gttcaggact tacaggcgta
    10741 tcaagcacat cataaagcac ctgtcttaat gattcatttt ctgttgtatc cgccgcatgc
    10801 tgaacaagat gacgcacaag cgttttcggt acagacgcat tgacgccgta catagacatg
    10861 tgatctccgt tatatgtcgc ccaccctaaa gcaagctccg acatatcgcc tgttccgatc
    10921 accataccgg cttcccgatt cgcaatatcc ataagcacct gagtccgctc tcttgcctgt
    10981 gcattttcat aagttacatc atgcacatcg ggatcatgtc cgatatctct gaaatgaacg
    11041 ttcaccgctt ctccgatcgg cacttcaatc aacgtcgctc cggtctgtct cgtcatctca
    11101 cacgcattat tatatgtgcg gtctgttgtc ccaaagcacg gcatcgtaac cgcaataatc
    11161 tcttttttat cccgtcccag catatcgaac gcctttgccg tgacaagcag agccaatgta
    11221 gaatccagac cgcccgaaat gccgacaacc gctgattttg cattcgtatg cgacaacctt
    11281 tttttcagac ccattgcctg aattgtcaag atttcgtcac aaatatgcgc tcgcgccgcc
    11341 tcgtcagacg gcacgaacgg atgctgtgaa aagtttctcg taagaacagt atcgcatatt
    11401 cccacatgaa acggcacaac taccggacaa ccgcaattta gcttttccgg tgaaaacgtc
    11461 gtatttttac gtctctctcc tacaattttt tcaagatcga tctctgaata aatgatctcg
    11521 tttttataac gttctgactg ccggaggatc gttccgtctt ccgcaatgat attatgtcct
    11581 ccgaaaacga gatccgtcgt tgactctcct tctcccgcgt tcgcatagac atatcccgcg
    11641 atcaggcgcg cggactgacc tgcgatcaga cgggttctgt aacttccttt tccgaccgtt
    11701 tcgtcacttg cagaacagtt gacaatgatc gtcgctcctg ccaaagcctc ctcaatactc
    11761 ggaggaacag gagaccagac gtcttcgcat atttccgcgc ctactataag atccgccgtc
    11821 tcatcatcct gaaacaggat cttcggcccg aaaggaacct tttctccgtc aaactgaatc
    11881 cacctcacac tgtccggtcc cggagtgaat tgacgcatct cgtaaaactc tccgtaattg
    11941 ggaagaaatg ttttcgttgt aaatccgatc acttttccat cgttgactgc cgccgccgtg
    12001 ttataaagct tcccgccttc tgtaagcggc attcccacaa acgccagaat gtttttgcct
    12061 tttgtatgcc ctgcaatctt tttcactgcg ccgtatgccg cgttcagcaa aacgttctgc
    12121 gtaaaaagat ctccgcatgt atatcctgtt acgcataatt cgggaaatgc gacgatcttc
    12181 gccccttttt catatgcctg atcgatcgcc ctgcatatct gttctgtatt atattccaca
    12241 tctgccactc gaatatcagg cgtggcagcc gctactttca caaaaccatg tctcatcttt
    12301 aactccacct ttccgctccc ggcttttctg gtttccccat catcggcaca agccgtctta
    12361 ctctattttc tatcttcatc cgtaataact gttttgcctg attttctgcc ttatctgctt
    12421 tttttcagta attctttcag ctcgtctaca gtgtaattat atgcttcatt acagaaatga
    12481 cattttactt cgatatcttt tccatcatcg atcattgact gaatatcttt tttaccgatg
    12541 ctgataattg ccttttcgat ccgcttcttt gaacagttgc aataaaattt tgcaggcatc
    12601 gtatctgtaa tctcaagatc aagtccttct aatacttgcg caagcatttc ttccggcgta
    12661 tgtccatgat cgagcataga agttacagat cggatgttct gaatattttc ttccagtttg
    12721 ttcaacactt catcttcgat aaacggcatt acctgcacaa tgaatccgcc tgcacagcgc
    12781 accgtattgt ctttttccat gagaacgccc aaccctacgg aagacggaac ctgttctgac
    12841 gtagcgaaat aatacgtcag atcttccgcg atttcccctg tctgaagaat cgtctgtcct
    12901 gaatacggct ctttaagccc catgtctttt atcaccgtca ttacgccctg tccgagcgct
    12961 ccgcctacat cgagttttcc gtttttcgga ggaagaatca cattcggttc atgcacataa
    13021 cctttgacat tccctttgct gtccgctgtc actgtaagac ctccgatcgg accggaacat
    13081 ttgatctgta tcgtcagcat atctgtgtca tttttcatca tgcttcccat cattgctccg
    13141 cctgtgagca gtcttccgag agccgccgtt gcgacaggac ttgtctcatg acgcttgcga
    13201 gcctcctctg tcagttctgt tgtcactgcc gcaaatgcgc ggatctgtgc attcgccgca
    13261 gatgctctta caatataatc tttcatcaat ctttctcctg ttctgtttta ttttatcatt
    13321 acttcattct attttctttt tgtcgcttgc tctgtttatt tatcatgaat ttatcgttat
    13381 tttattatta ctcattctgt ttattttccg ttttcacggg cgatcacgca tattctctcg
    13441 ctcgtatcgt tcggcgtctc tttcgtatcc gcatcgtaag cctctataaa tacaagacct
    13501 gcacgttcca gaagttcttg tatctcggca agcgtatacc ctctttgata atgcgtctcc
    13561 gtatatctcc gatataaact tccgttttcg ccaccctctt cttctgaaaa catagtatct
    13621 tccaagtcac ccgctccttc ttctgaaagc agagcatctt ccgaatctgc cgttctttct
    13681 gaaacatatg cattttccat agggttaaaa tcttccgcga taaacagcgt cagatcgtac
    13741 tcgttgatct gttctttctc atagtatctg ttatcccaaa taaagctgca cacgccg
//
LOCUS       DBMDXF010000030        13693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1308_length_13693_cov_1.876692, whole genome shotgun sequence.
ACCESSION   DBMDXF010000030 DBMDXF010000000
VERSION     DBMDXF010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1308_length_13693_cov_1.876692"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="ACZPD4_02320"
     CDS             complement(<1..1028)
                     /locus_tag="ACZPD4_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1377947.1"
                     /translation="MESFQVYKDMKARTNGEIYIGVVGPVRTGKSTFIKRFMDLLVLP
                     NMTDIHAKERTQDELPQSASGTTIMTTEPKFVPKEAAGVKLSDDLEVKIRMIDCVGFM
                     AEGASGHIEKDEERQVKTPWFEYEIPFTKAAAIGTQKVIRDHATIGIVVTTDGSVTDL
                     PRENYIQAEERTVKELKAIGKPFMILLNCKKPYTEESKKLQEELREKYETAVLPVNCE
                     QMKEEDIHEIMRQVLYEFPVTEVEFYVPKWVEMLSREHKIKQDLFEHVRKIMETMDDI
                     RSVVSRSFEAEGPYIERILTEKIEMDTGKVQVKIEFAESYYYEVISEVTGEEIHGEFE
                     IGGVTAQF"
     gene            complement(1087..2100)
                     /locus_tag="ACZPD4_02325"
     CDS             complement(1087..2100)
                     /locus_tag="ACZPD4_02325"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR1377948.1"
                     /translation="MANVGVIGAGSWGTALSVLLADNGHHVTIWSIDEKEVEMLDKKR
                     EHLTKLPGVKLSDGMTITGDLESAVKGKDFLVLAVPSPFTRATAKKMSAYVGKGQIIV
                     DVAKGIEESTLLTLSAQIEEEIPQADVAVLSGPSHAEEVGRKLPTTCVIGAKTKKTAE
                     YLQSAFINKVFRVYTSPDILGIELGGSLKNVIALAAGIADGLGYGDNTKAALITRGIA
                     EIARLGVKMGGKIETFTGLTGIGDLIVTCASVHSRNRKAGYLIGQGRSMQEAMDEVQM
                     VVEGVYSAKAAAKLAKKYDVPMPIVEEVNKVLFEGKSPAEAVDDLMLRESRSEHRALQ
                     WEK"
     gene            complement(2104..>2524)
                     /locus_tag="ACZPD4_02330"
     CDS             complement(2104..>2524)
                     /locus_tag="ACZPD4_02330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR1377949.1"
                     /translation="PLLVMYAGAGVTLGHNFPFYMNFKGGKGIAVMAGLVFINSVWHM
                     PQSLFLILVTALFFFIPVIVTRYISVGSLCAYTAFLIEMILFGQWGWFDMEAGYCYEL
                     YGIAVLLTALAWYRHRENIKRLVKGTENKFGSKRKGH"
     assembly_gap    2525..2534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2535..2763)
                     /locus_tag="ACZPD4_02335"
     CDS             complement(<2535..2763)
                     /locus_tag="ACZPD4_02335"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR1377950.1"
                     /translation="MERLICLAVGYVCGLFQTGYLVGKMNHIDIRKEGSGNSGTTNAL
                     RVLGWKAGIFTFLGDVLKCVAAYFIVRFMYRG"
     gene            complement(2770..4095)
                     /gene="der"
                     /locus_tag="ACZPD4_02340"
     CDS             complement(2770..4095)
                     /gene="der"
                     /locus_tag="ACZPD4_02340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR1377951.1"
                     /translation="MSKPVVAIVGRPNVGKSTLFNALAGEMISIVKDTPGVTRDRIYA
                     DVTWLDKEFTMIDTGGIEPDSKDIILSQMREQAQIAIDTADVIIFITDVKQGLVDADS
                     KVADMLRRSGKPVVLVVNKVDNFDKYMADVYEFYNLGIGDPVPISAASRLGLGDMLDI
                     VAENFPEGSAQAEDDDRPRVAIVGKPNVGKSSIINKLLGENRVIVSDIAGTTRDAIDT
                     EILHNGKEYIFIDTAGLRRKNKIKEELERYSIIRTVTAVERADVVLMVIDATEGVTEQ
                     DAKIAGIAHERGKGVIIVVNKWDAIEKNDRTMREYESDIRQVLSYMPYAEIMYVSAAT
                     GQRLNRLYDMIDMVIENQTLRIATGVLNEIMTEAVAMQQPPSDKGKRLKLYYITQVAV
                     KPPTFVIFVNDKELMHFSYTRYLENKIREAFGFRGTSLKFFIRERKEKG"
     gene            complement(4108..5466)
                     /locus_tag="ACZPD4_02345"
     CDS             complement(4108..5466)
                     /locus_tag="ACZPD4_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1377952.1"
                     /translation="MTGKKHEHIVKSIMPGSIAEELGIEPGDKLLSIDGQEIEDIFDY
                     QFYVEDEEIVLLIEKQDGEQWELEIEKDADEQLGIEFGQGLMDEYRSCRNKCMFCFID
                     QMPEGMRDTLYFKDDDSRLSFLQGNYVTLTNMSDHDIERIIRYRLEPINISFQTTNPQ
                     LRCKMLHNRFAGDALKKVDRLFEGGIEMNGQIVLCKGENDGAELERSIKDLTAYIPLL
                     RSVSVVPVGLTKFRDGLYHLEPFTKEDAKEVLSVIHKWQDRIYEQYGIHFIHAGDEWY
                     LLAGQEVPEEERYDGYLQLENGVGMLRLLFEEFTEGYKSLTGDERQEELSIATGKLAY
                     PYISAMAEKIEEKFPNLEIHVFSIRNDFFGERITVSGLITAQDLTAQLKGERLGSRLL
                     IPCNMLKTDEDVFLDDFTVRQVSDALQVPIDIVKSSGQDFIDAVIGEKQTDPDCKTER
                     LI"
     gene            5687..6868
                     /locus_tag="ACZPD4_02350"
     CDS             5687..6868
                     /locus_tag="ACZPD4_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1377953.1"
                     /translation="MSNIELLEKTLPAAPLKLIAMESCRELGQKVNDYIVSFRENTIN
                     EVSESSLYVNYKSNNYLVDCCCPRFGTGEAKGLLKETIRGTDLFIMTDVCNHNLTYTV
                     NGHLNHMSPDDHFQDLKRIISAATGKAKRINVIMPFLYESRQHKRTKRESLDCALALE
                     ELNAMGVSNIVTFDAHDPRVQNAIPLSGFDSFNPPYQFMKALFRAVPDLIPDKDHLMI
                     ISPDEGAMHRAVYFSNILGVDMGMFYKRRDYSTIINGKNPIVAHEFLGDDVAGKDVII
                     VDDMIASGGSMLDVAKQLKERKAGRVFVCTTFGLFTEGFEKFDEYYEKGYINKVITTN
                     LTYLPAELYKKPYFVKADMSKFLALIIDSLNHDITIGAVMNPTDKIHALLEKYARGEQ
                     I"
     gene            complement(7048..8262)
                     /gene="ilvA"
                     /locus_tag="ACZPD4_02355"
     CDS             complement(7048..8262)
                     /gene="ilvA"
                     /locus_tag="ACZPD4_02355"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR1377954.1"
                     /translation="MMTLEKFEEASELVKKVTNSTKLVYSDYLSEQSGGKVYLKPENM
                     QHTGAYKIRGAYYKISTLSDEERGRGLITASAGNHAQGVAFAAKKFGCKAVIVMPTVT
                     PLIKVNRTKSYGAEVILHGDVYDDAYAYACELAEKEGYTFVHPFNDIDVATGQGTIAM
                     EIVQELPTVDYILVPIGGGGLVSGVATLAKMLNPKIKVIGVEPAEAASMKAALAAGEV
                     VQLESANTIADGTAVKAVGDKILPFVKENVDDVLLVEDDELIGAFLDMVENHKMIVEN
                     SGLLSVAALKQLDCKGKKVVCVLSGGNMDVITMSSIVQHGLIQRDRIFSVSVLLPDKP
                     GELVQVAQTIAEAQGNVIKLEHNQFVSTNRNAAVELRITMEAFGTEHKQEILKALEKK
                     GYRPRLIGAKLY"
     gene            complement(8259..9197)
                     /gene="ftsY"
                     /locus_tag="ACZPD4_02360"
     CDS             complement(8259..9197)
                     /gene="ftsY"
                     /locus_tag="ACZPD4_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1377955.1"
                     /translation="MEEKKGFFRRLVSGLAKTRDNIVSGMDSIFHGFSKIDEEFYEEL
                     EETLIMGDLGVHSTMEILEDLRAKVKERHIKEPIECRQLLIDSIREQMDVGETAYEFE
                     NRTSVVFVIGVNGVGKTTTIGKLAGKLKSQGKKVVLAAADTFRAAAGEQLREWANRAD
                     ADMIGGQEGADPAAVIYDAVAAAKARHADVLLCDTAGRLHNKKNLMEELKKINRVIDR
                     EYPEAFRETLVVLDATTGQNALAQAKEFNEVADITGVILTKMDGTAKGGIAVAIQAEL
                     GIPVKYIGVGETIDDLQKFDADEFVNALFYREDEEK"
     gene            complement(9238..12798)
                     /gene="smc"
                     /locus_tag="ACZPD4_02365"
     CDS             complement(9238..12798)
                     /gene="smc"
                     /locus_tag="ACZPD4_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1377956.1"
                     /translation="MYLKSIEVQGFKSFANKIKFDFHNGITGIVGPNGSGKSNVADAV
                     RWVLGEQRVKQLRGGTMQDVIFSGTENRKPLSYASVAITLDNADHKLPVDYEEVTVTR
                     KLYRSGESEYLINGASCRLKDINEMFYDTGIGKEGYSIIGQGQIDKILSGKPEERREL
                     FDEAAGIVKFKRRKNLSVKKLEEERMNLTRVNDILQELEKQLGPLEKQSETAKEYLKK
                     KEELKTYDINMFLLEEERIRDCLKETQKKLEIASLELEESNISYDKMKTEYEAIEEEV
                     EEIDLAIETAKNRLNETNLLKQQLEGQINVLREQINTARMNDEHYDNRLNTVQSEINM
                     RKEQKASFEEEKKNLSEKLREASQADTVVKKRLIDVQTQIAEHTEEIEQRKQEIMDLL
                     GNRASTKAKIQHFDTTRDQISTRKSVLARNILEVSAKAKEQEEQLHEHEERLSEIQDK
                     IRLYNMQITENEQKIQKLQEELNVRQEKLNVGRTAYHREASRLESLKNITERYDGYGN
                     SIRKVMANKDKNPGLIGVVADIIKVDKEYEIAIETALGGSIQNIVTDNEDTAKKMISF
                     LKTNKFGRATFLPLTSMRGGGGIRNEEALKEPGVIGTADKLVYVEARFTGLAEQLLGR
                     TLVVRTIDDGILIARKYKQSIRLVTLEGELINPGGSMTGGAFKNSSNLLSRRREIEEF
                     EKTVAMLKKDMDAAEADVSRIKSERAGCYNMVDDIRQELRKASVIENTAKMNAEQTKT
                     RMEEAKQSCAGYVAEQGKLERELGEIIENEESIRMELEVSENLEKELNLRIEELHALL
                     EKERDTESEQLKISEESHLSLAGMEQQNLFVSENISRIEEEIVKFQAELEELDKNKDH
                     ASDEIKEKEDKIKELRGTIDNSRELFDEIEKEIKEQTEGRDALNRKHKQFLQMREDLS
                     KHISALDKECFHLDSQKQSYEAASEKQMNYMWEEYEITYNHAMKLRDENLTDLAYMKR
                     QIQELKNEIRKLGTVNVNAIEDFKNISERYAFLKNQHDDLVEAEQTLMQIIDELDAAM
                     RKQFAEQFLKIKEEFNTVFRQLFGGGKGTLELMEDEDILEAGIRIIAQPPGKKLQNMM
                     QLSGGEKALTAISLLFAIQNLKPSPFCLLDEIEAALDDNNVTRFAQYLHKLTKNTQFI
                     VITHRRGTMTAADRLYGITMQEKGVSTLVSVDLLEDKLDR"
     gene            complement(12811..13497)
                     /gene="rnc"
                     /locus_tag="ACZPD4_02370"
     CDS             complement(12811..13497)
                     /gene="rnc"
                     /locus_tag="ACZPD4_02370"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1377957.1"
                     /translation="MHKKLKELEQKIGYRFRNFSFLEQAMMHSSYTNEHRMEKYRCNE
                     RLEFLGDAVLELISSEFLFRQSPKVPEGELTKTRASMVCEQSLAMCARDIDLGSYLLL
                     GKGEEVTGGRFRDSVTSDALEALIGAIYLDGGFTNAKEFIHRFVLTDQEDKKLFYDSK
                     TILQEIVQAEKLGEIEYHLIGEEGPDHNKSFSVELTIRGRSYGKGKGRTKKAAEQQAA
                     YEAIHVLKKR"
     gene            complement(13590..>13693)
                     /locus_tag="ACZPD4_02375"
     CDS             complement(13590..>13693)
                     /locus_tag="ACZPD4_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1377958.1"
                     /translation="GIEEEFDIEIDNEEAEKITTVQDAVDQIKKAVE"
BASE COUNT         3430 a         3583 c         2224 g         4446 t
ORIGIN      
        1 aagaattgcg cggtaacgcc gccaatttca aattcgccgt gaatctcttc tcctgtcact
       61 tcactgatca cttcatagta ataagattcg gcaaattcaa ttttcacctg tacttttccc
      121 gtatccatct cgatcttttc cgtcaggata cgctctatgt acggtccttc agcctcaaaa
      181 gaccgcgaca cgacacttcg gatatcatcc atcgtctcca tgatctttct tacatgctca
      241 aaaagatctt gtttgatctt atgttccctc gaaagcattt ccacccattt cggcacataa
      301 aactcgacct ctgtgaccgg aaattcatag agaacctgac gcataatttc atggatatcc
      361 tcttctttca tctgttcaca attcacaggc aatactgccg tttcgtattt ttcacgaagt
      421 tcttcctgaa gcttcttaga ttcttctgta tatggtttct tacagttaag caatatcata
      481 aacggctttc ctatcgcttt gagttctttt accgtccttt cttctgcctg aatataattt
      541 tccctcggca aatccgtaac gcttccgtct gtcgttacaa caatccctat tgtagcatga
      601 tcccggatca ctttctgcgt cccgattgca gccgctttcg taaacgggat ctcatattca
      661 aaccacggtg tctttacctg ccgctcttcg tccttttcaa tatggccgga tgctccttct
      721 gccataaaac cgacacagtc gatcattcgt atcttcactt ccagatcatc tgacagcttc
      781 acccccgccg cctctttcgg aacgaacttc ggttctgtag tcataattgt cgtaccggaa
      841 gcagactgcg gcaattcatc ttgcgtccgt tcttttgcat gtatatctgt catattcgga
      901 agcactaaaa gatccataaa ccgcttgata aatgttgatt tgcctgtccg aaccggtccg
      961 actaccccga tataaatttc cccgttcgtc ctcgccttca tatctttgta tacttgaaat
     1021 gattccataa aaataccccc ttgttctgct gatacaattt atgcatccgc aaacgggggt
     1081 atgactttat ttttcccatt gaagcgctct atgctcggaa cgcgactctc taagcatcag
     1141 gtcatccaca gcttccgccg gtgatttccc ttcaaaaagc actttattga cttcttccac
     1201 gatcggcatg ggaacatcat atttttttgc cagcttcgcc gctgcctttg ccgaataaac
     1261 gccttcaaca accatctgaa cttcatccat tgcttcctgc attgaccttc cctgtccgat
     1321 aagatatcct gctttccggt ttctgctgtg gacgctggcg cacgtaacga tcaaatctcc
     1381 tattcccgtc aaacctgtga acgtctcgat cttcccgccc atcttcacgc ccagtctggc
     1441 aatttccgca attccgcggg taataagcgc tgcctttgta ttatctccgt aaccaaggcc
     1501 atctgcgatt cccgctgcaa gagcaatgac atttttcaaa gaaccgccaa gctcaattcc
     1561 taaaatatcc ggacttgtat atacacggaa cactttatta ataaacgcag actgcaaata
     1621 ttccgccgtt ttttttgttt ttgctccgat gacacatgtt gtcggaagct ttcttccgac
     1681 ttcttctgca tggcttggtc ctgaaagaac cgctacgtcc gcctgaggga tttcttcttc
     1741 gatctgagca gacaatgtca aaagtgtact ctcctcgatt ccctttgcca catcaacgat
     1801 aatctgacct ttcccgacgt aagctgacat cttcttcgca gttgcccttg taaacggcga
     1861 cggaactgca agtacaagaa agtcttttcc cttgactgcc gattccagat cacccgtgat
     1921 cgtcatgccg tcggaaagtt tcacccccgg aagcttcgtc aggtgttctc tctttttatc
     1981 cagcatttca acttcttttt catcgatcga ccagatcgtc acatgatgtc cgttgtcagc
     2041 cagaagaacc gataacgccg ttccccagct tcccgcaccg attactccta catttgccat
     2101 aaatcaatgc ccctttcttt ttgaaccgaa tttattttct gttcctttga ccagacgctt
     2161 tatgttctct ctatggcgat accatgctaa tgcagtcaaa agaactgcga tcccatatag
     2221 ttcataacag tagcctgctt ccatatcgaa ccatccccac tgtccaaata ggatcatttc
     2281 aattaaaaat gctgtgtatg cacaaagaga accgacagat atgtaccttg tcacgatcac
     2341 aggaataaaa aagaataatg ccgtaacaag tataagaaaa agactctgcg gcatatgcca
     2401 cacactgtta ataaagacaa gtcctgccat aaccgcaatt ccttttccgc ctttaaagtt
     2461 catataaaac ggaaagttat gtccgagagt cactcctgcc cctgcgtaca ttacaagcaa
     2521 cggannnnnn nnnntccccc gatacataaa acgtacaata aaatatgcag ccacacattt
     2581 taaaacatct ccgagaaaag tgaagatccc cgctttccat ccgagcacac gaagagcatt
     2641 tgtcgttccc gaatttccgc ttccctcttt tctgatatct atatggttca tcttaccgac
     2701 cagatatccg gtctgaaaaa gaccgcacac atatccgacc gcaagacaga tcaatcgttc
     2761 catatttctt tacccctttt ctttccgttc tcttataaag aatttcaggg aagttcctct
     2821 aaaaccaaac gcttctctga tcttattttc caaataccgc gtatatgaaa aatgcatcaa
     2881 ttccttatcg tttacaaaga taacaaaagt cggaggtttt accgcaactt gcgtaatata
     2941 ataaagcttc agtctttttc ctttatctga cggaggctgc tgcattgcca ctgcttctgt
     3001 cataatctcg tttaaaactc cggttgcgat ccgaagtgtc tgattctcga taaccatatc
     3061 gatcatatca tataaccggt tcaatctttg accggttgct gcggatacat acataatttc
     3121 cgcatacggc atataagaaa gcacctgacg gatatcgctt tcatactcac gcatcgttct
     3181 gtcgtttttc tcaatcgcat cccatttgtt cacaacgatg atcacgcctt ttccacgttc
     3241 atgagcaatt cccgcaatct ttgcatcctg ctccgtcact ccttctgtcg catcgataac
     3301 cataagcact acgtctgctc tttccacggc tgttaccgta cggataatac tgtagcgttc
     3361 cagttcttct ttgattttat tctttctgcg aagtcctgcc gtatcaataa aaatatattc
     3421 ttttccgtta tgaagaatct cagtatcaat ggcgtctctt gttgttcccg cgatatccga
     3481 gacgattact cgattctctc cgagcagttt attgataatc gacgactttc cgacgttcgg
     3541 tttccctacg atcgcaaccc gcgggcggtc atcatcttcc gcctgagcgc ttccttccgg
     3601 aaagttttcc gcgacaatgt caagcatatc gccaagtccc aatctggatg ctgccgagat
     3661 cggaaccgga tccccgatac caagattata aaactcatat acatctgcca tatatttgtc
     3721 aaaattatcc actttattta caacaaggac aaccggcttt ccggatcttc tgagcatgtc
     3781 cgcgacttta gaatccgcat ccacaagtcc ctgcttcaca tcagttataa aaataataac
     3841 atccgccgta tcaatcgcaa tctgagcctg ctctctcatc tgtgataaaa tgatgtcttt
     3901 gctgtccggt tctataccgc ctgtatcgat cattgtaaat tctttatcaa gccatgtcac
     3961 atccgcataa atcctgtctc ttgtcactcc cggagtatct ttcacaatag aaatcatctc
     4021 tcctgccaaa gcattaaaaa gggttgactt tccgacattc gggcggccga caatggcaac
     4081 tactggttta ctcatattat tttcctttca aatcaatctt tcggttttgc agtccggatc
     4141 tgtctgtttt tctccgatca ccgcatctat aaaatcctgt ccgcttgatt ttacaatatc
     4201 aataggcact tgtaaagcgt cggatacttg tcttaccgta aaatcatcaa gaaacacatc
     4261 ttcatctgtc tttaacatat tgcacggaat gagcagtctg ctgccaagac gctctccttt
     4321 aagctgcgct gtcagatcct gcgccgtgat cagtccggac acagtaatgc gttctccgaa
     4381 aaagtcgttt cggatagaaa agacatgaat ttccaaatta ggaaatttct cttctatctt
     4441 ttccgccatg gcgcttatat aaggatatgc cagctttcct gtggcaatcg acagttcttc
     4501 ctgccgctcg tctcctgtaa ggcttttata cccttctgta aattcttcaa aaagaagacg
     4561 gagcattccg accccatttt ccaactgaag atatccgtca taccgctctt cctccggcac
     4621 ctcctgtcct gcgagcaaat accactcatc ccctgcatga atgaaatgaa ttccgtactg
     4681 ctcataaatg cgatcctgcc atttgtgtat aactgataat acttctttgg catcctcttt
     4741 cgtaaacggc tctaaatgat acagaccgtc tctgaacttt gtaagcccta ccggaacgac
     4801 agaaacgctt cgaagaagcg gaatatatgc cgtcaggtct ttaatagaac gttccagctc
     4861 agccccgtcg ttttctcctt tgcacagtac gatctgtccg ttcatttcaa taccgccttc
     4921 aaaaagtctg tctacttttt tcagcgcatc tcccgcaaaa cgattgtgaa gcatcttgca
     4981 gcgtagttgt ggattcgtcg tttgaaacga aatattgatc ggttctaacc tatacctaat
     5041 aatccgctca atatcatgat cactcatatt tgtaagcgtc acataatttc cctgtaaaaa
     5101 agacaatctt gaatcatcgt ctttaaaata aagagtatct ctcattccct ccggcatctg
     5161 atcgatgaag cagaacatgc acttattccg gcaggaacga tactcatcca tcaacccctg
     5221 cccgaactct attccaagct gctcgtctgc atctttttca atctcaagct cccactgttc
     5281 tccgtcctgt ttctcaatca aaagtacgat ttcttcgtct tccacgtaaa actgataatc
     5341 aaaaatatct tcaatctcct gaccgtcgat cgaaagaagt ttatctccgg gttcaatgcc
     5401 taattcctcc gcaatactgc cgggcatgat tgatttgaca atatgctcat gtttttttcc
     5461 tgtcatttgt tattcccctt atttgctgtt cattctatca catactataa cacatttaaa
     5521 cctcttcgca aagactgtac ttcaccaata caacatacat acaaaagaag cggcgcttga
     5581 atttcaggct gaaaaatgat atgattctag tgctgtaaag tcgtttgcat ttttctatgc
     5641 tccggcttaa aactcaattt atactattat ttaacaggag aatactatgt caaatatcga
     5701 acttttagaa aaaactcttc cggccgcacc gctaaaactc attgcaatgg aaagctgcag
     5761 agaactcgga caaaaagtaa atgattatat tgtttctttt cgtgaaaata caatcaacga
     5821 agtatccgaa tcttctctgt atgtaaacta caagtcaaac aactatctcg tagactgctg
     5881 ctgtccccgt tttggcacag gagaagcaaa aggacttctt aaagaaacta tccggggaac
     5941 agatcttttc atcatgaccg acgtttgcaa ccacaacctt acttacactg taaacggaca
     6001 tctaaaccac atgtctcctg atgatcattt ccaggacctg aaacgaatca tctccgctgc
     6061 aaccggcaaa gcaaagcgta ttaacgtcat tatgcctttc ctgtacgaaa gccgccagca
     6121 caaacgtaca aaacgcgaat ctcttgactg tgcgcttgct cttgaagaat taaacgcaat
     6181 gggagtttca aacatcgtca cttttgacgc ccacgatccc cgcgtacaaa acgcgatccc
     6241 tctaagcgga tttgacagct tcaatccgcc gtaccaattt atgaaagcgc tgtttcgcgc
     6301 cgttcctgat cttatccccg ataaagatca ccttatgatc atcagccctg atgagggagc
     6361 gatgcaccgc gccgtttatt tttccaatat tctcggtgtc gatatgggaa tgttttataa
     6421 acgccgtgac tattctacaa tcatcaatgg taagaatccg attgtggctc atgaatttct
     6481 cggcgatgac gttgccggaa aagatgttat catcgtagat gatatgatcg cttccggagg
     6541 aagtatgctt gatgtcgcaa aacagttaaa agaaagaaaa gcaggccgtg tatttgtatg
     6601 tacaactttt ggtttattca ccgaaggatt tgaaaaattt gacgaatact atgaaaaggg
     6661 atatatcaat aaagttatta cgacaaacct tacatatctg cctgcagaat tatacaaaaa
     6721 accatatttc gtaaaagcag atatgagtaa gtttctcgct ttgatcattg attctcttaa
     6781 tcacgatatt acaatcggtg ctgtaatgaa tccgacagac aaaattcatg ctctgcttga
     6841 aaaatatgcc cgcggcgaac agatttgatc gtgcatacag tataaaatat attgtaacta
     6901 attgatttta aaagaccggc gcttgaatgt ttgtttgcta tacaatgaaa cttagagcta
     6961 taaaaacggt acagtcaaaa gattctttta tcttgagact gtaccgtttt tatagtttcc
     7021 aaatgtatcc ctcaccgctt tgcaaactta atacagttta gcaccgatca atctcggacg
     7081 gtatcctttt ttctctaatg cttttagtat ctcctgcttg tgttctgttc caaatgcctc
     7141 catcgtaata cgaagctcta ctgccgcatt tctgtttgta cttacaaatt ggttatgctc
     7201 cagcttaatt acatttccct gagcttctgc gattgtctgc gccacctgga caagttcacc
     7261 cggtttgtca ggaagaagta cagatacaga aaaaatcctg tcgcgttgga tcaatccatg
     7321 ctgtacaata gatgacatcg taattacgtc catattaccg ccgcttaata cacagactac
     7381 ctttttccct ttgcagtcaa gctgcttcaa tgccgctact gacaaaagac ctgaattttc
     7441 aacgatcatc ttatgattct ctaccatgtc aagaaatgcg ccgatcaatt catcatcttc
     7501 tacaagaaga acgtcgtcta cattttcctt aacaaagggc agaattttat ctcctacagc
     7561 tttcacggcc gttccgtccg cgatcgtatt ggcgctttca agttgtacga cttcgcctgc
     7621 cgcaagcgcc gctttcatac ttgccgcctc tgccggttct actccgatta cttttatttt
     7681 cggattcaac atcttagcca gcgtcgcaac gccggaaaca agtccgcctc ctccgatcgg
     7741 aacaagaatg taatcaacag tcggaagttc ctgaacgatt tccattgcga tcgttccctg
     7801 gcctgttgct acatctatgt cattaaacgg atgtacaaac gtatatccct ctttttctgc
     7861 cagctcacac gcataggcat aagcatcgtc atagacatct ccgtgcagga tcacttccgc
     7921 gccgtagctc tttgtacggt tcacttttat aagaggtgta accgtaggca taacgataac
     7981 tgctttacag ccaaattttt ttgcggcaaa ggcaacgccc tgagcgtgat ttcccgccga
     8041 tgccgtgatc agtcctctgc ctcgttcttc atccgataac gtactgattt tataataagc
     8101 tcctctgatc ttgtatgctc cggtatgctg catattttcc ggctttaaat aaacctttcc
     8161 tccgctttgc tcgcttaaat aatcactgta cacaagcttt gtagaattcg tcactttttt
     8221 tacaagctcg ctggcttctt caaatttctc caatgtcatc atttttcttc atcctctctg
     8281 taaaacaatg cgtttacaaa ctcatctgca tcaaatttct gaagatcgtc tatcgtctct
     8341 ccgactccga tatattttac gggaattcca agttctgcct gaatcgcaac tgcaattcca
     8401 ccttttgccg ttccgtccat ctttgtaaga ataacacctg taatgtctgc cacttcatta
     8461 aactcttttg cctgtgccaa agcattttgt cctgttgtcg catctaatac gacaagtgtc
     8521 tcccgaaaag cttccggata ctctctgtcg atcacacggt tgatcttctt aagttcctcc
     8581 atcaggttct ttttattgtg aagtcttcct gccgtatcac agagaagcac atctgcatga
     8641 cgtgcttttg ccgcagctac cgcgtcatag attacagctg ccggatctgc tccttcctgc
     8701 cctccgatca tatctgcatc ggcacggttc gcccattctc gaagctgttc tcccgccgca
     8761 gcacgaaaag tatcggctgc cgccaaaaca acctttttcc cctgactttt caactttccc
     8821 gccagttttc cgatcgtagt tgttttcccg actccgttta ctccgatcac aaacacgaca
     8881 gatgttctgt tttcaaactc atatgccgtc tctcctacat ccatttgttc ccggatactg
     8941 tcaatcaata attgtctgca ctcaatcggt tctttgatat gtctttcttt tacttttgcc
     9001 cggaggtctt ctaatatttc cattgtagag tgtactccga gatcgcccat gatcaacgtc
     9061 tcttccagct cctcataaaa ctcctcgtct atttttgaaa agccatgaaa aatactgtcc
     9121 atcccggata caatattatc tcttgtcttt gcaagacctg atacaagacg cctgaaaaat
     9181 cctttttttt cttccattta tctaattaat tcctttctct gattccttta tcaattctca
     9241 gcgatccaat ttatcttcca aaagatcgac tgaaacaagc gttgaaacgc ctttttcctg
     9301 catcgtgatt ccatacagtc tatctgctgc cgtcatcgtt cctctacgat gcgtaattac
     9361 gataaactgc gtatttttcg tcagtttatg cagatattgt gcaaagcggg tcacgttatt
     9421 atcatccagc gccgcttcaa tctcatcaag aagacaaaac ggcgacggtt ttaaattctg
     9481 gatcgcaaat aataaagaaa ttgccgtcaa cgctttttcg ccgccggaaa gctgcatcat
     9541 attttgaagt tttttccctg gcggctgggc aatgatacgg attcccgcct ccaaaatatc
     9601 ctcatcttcc atcaactcca atgttccttt tccgcctccg aacaactgtc ggaacactgt
     9661 gttgaactct tcttttattt ttaaaaactg ctcggcaaac tgttttctca ttgcagcatc
     9721 taattcatca atgatctgca tcagcgtctg ctccgcctcg acaaggtcgt catgctgatt
     9781 tttcaaaaat gcatatcttt cggaaatatt tttaaaatct tcaatcgcat tgacattgac
     9841 tgtgccgagt tttcggatct catttttcaa ctcttgtatc tgacgcttca tatacgcaag
     9901 atccgtcaga ttttcgtcac gaagcttcat cgcatgattg tatgtgatct catattcttc
     9961 ccacatataa ttcatctgct tttcagacgc cgcctcgtaa gattgttttt gactgtccag
    10021 gtggaaacat tctttatcga gagccgaaat atgttttgac aagtcttccc gcatttgcag
    10081 aaactgttta tgcttcctgt ttaatgcatc tcttccctct gtctgttctt ttatttcttt
    10141 ttcaatttca tcaaacaatt ctcttgaatt atcaatcgtt ccccgaagct cttttatctt
    10201 gtcttccttt tccttaatct catctgacgc atgatcttta tttttatcca attcttccag
    10261 ttctgcctga aacttgacga tctcttcttc aatacgtgaa atattttccg atacgaaaag
    10321 attctgctgt tccattcctg ccaaagataa atgtgattct tctgagatct taagctgttc
    10381 actctccgta tctctttctt tttcaagcag agcatgaagt tcttcgatcc gaagattgag
    10441 ttctttttct aaattttcag atacctcaag ttccatcctg atcgactctt cgttctctat
    10501 tatctctcct aactctcttt caagctttcc ttgttctgcg acataacctg cacagctttg
    10561 ttttgcctcc tccatacgcg tcttcgtctg ctcggcattc atcttcgctg tattttcgat
    10621 gaccgatgcc tttctgagtt cctgacggat atcatctacc atattgtagc atcccgcgcg
    10681 ttcagattta atcctgctta catctgcctc ggcggcatcc atatcttttt tcagcatggc
    10741 taccgtcttt tcaaattctt cgatctctcg tcttctgctc agcaggttgc tggaattttt
    10801 aaatgcgcct cccgtcatcg atcctcccgg attgatcaat tctccttcca aagtaacaag
    10861 tctaatcgac tgcttatatt ttcttgcaat gagaattccg tcatcgatcg ttctaacgac
    10921 aagggttctt cctaacaact gctctgccag tccggtaaaa cgcgcctcta cataaacaag
    10981 cttatctgcc gtaccgatca ctcccggttc tttaagcgct tcttcattgc gaattccgcc
    11041 tccgcctctc atacttgtaa gcggcaaaaa tgttgcccgg ccgaacttat tcgtctttaa
    11101 aaaagaaatc attttcttgg ctgtatcttc attatccgta acaatatttt gaatacttcc
    11161 gcccaaagca gtctcaatcg caatctcata ttctttatcg actttgataa tatctgcaac
    11221 aacgccgatc aatcccggat ttttatcctt atttgccatc actttacgga tactgtttcc
    11281 atatccatca taacgctccg taatattttt cagtgattcc agcctggatg cctctctgtg
    11341 atatgctgtt cgtccgacat tcaatttttc ctgtctgaca tttaactctt cttgaagttt
    11401 ttgtatcttc tgttcatttt cggtaatctg catattatag agccgaatct tatcttgaat
    11461 ctcagacaac cgctcttcat gttcatgcag ctgctcttcc tgctctttcg cctttgccga
    11521 cacttctaaa atatttcttg caagcacgga ttttcttgtt gatatttgat ctctcgttgt
    11581 atcaaaatgt tggatttttg ctttcgtaga agctctgttt ccaagaagat ccataatttc
    11641 ctgtttccgc tgttcgatct cttctgtatg ctccgcaatc tgtgtctgca catcgatcag
    11701 tcgttttttt acaaccgtat ctgcctgcga cgcttcacgc agcttttccg aaagattttt
    11761 cttttcttct tcaaaagacg ctttctgctc ttttctcata ttgatttccg attggacggt
    11821 attcaaccgg ttatcataat gctcatcatt catacgagcc gtattgatct gttctctcaa
    11881 tacattgatc tgaccttcca gttgctgctt cagtaaattt gtttcattca aacgattttt
    11941 tgcagtttcg attgcaaggt cgatctcttc tacttcctct tcaatcgcct catactccgt
    12001 tttcatctta tcataactga tatttgactc ttctaattct aatgatgcga tctccagttt
    12061 cttttgtgtt tcttttaaac agtcacggat cctctcttct tccaaaagga acatattaat
    12121 atcatatgtc tttaactctt cctttttctt taaatactct ttcgctgttt ccgactgttt
    12181 ttccaaaggt cccaactgct tttccagttc ctgtaaaata tcgtttacgc gggttaagtt
    12241 catacgttct tcttcaagtt tctttaccga taggtttttt ctccgcttaa actttacgat
    12301 tccggcagcc tcgtcgaaca attctcttct ttcttccggc ttcccgctta atattttatc
    12361 gatctgtccc tgacctataa tcgaatatcc ttctttaccg atacctgtgt cataaaacat
    12421 ttcattaata tctttcagcc gacaggacgc tccgttgatc agatattcac tttctcctga
    12481 acggtacagc tttctcgtca cagtaacttc ttcataatca acaggcaatt tgtgatccgc
    12541 attatccaat gtgatcgcta cagaggcata acttaacggt ttcctgttct ctgttcccga
    12601 aaagattacg tcctgcatcg taccgccgcg gagctgtttt acccgttgct ctcccagtac
    12661 ccagcgaact gcatccgcaa cattgctctt tccgcttcca tttggtccga caatacctgt
    12721 aattccgtta tgaaagtcaa atttaatctt atttgcaaag gacttaaacc cctgcacttc
    12781 tatacttttt aaatacatag aacacctgct ttatcttttt ttcagcacat gaatcgcctc
    12841 ataggcagcc tgctgttccg ccgctttttt agtccttcct ttgcctttac cgtacgacct
    12901 gccccggatc gtcagttcca cagaaaatga tttgttatga tcaggtcctt cctcgcctat
    12961 cagatgatat tctatctctc ctagtttttc agcctgaaca atctcttgaa ggatagtttt
    13021 actatcataa aagagtttct tatcttcctg atccgtcaaa acaaaacgat gtataaactc
    13081 ttttgcatta gtaaaaccac catccagata tattgctccg atcaaagctt ccagtgcatc
    13141 tgaagtcaca gaatcacgaa aacgccctcc tgttacctct tccccttttc cgagaagaag
    13201 atagcttcca agatcgatgt ctctcgcgca catagccaat gactgctcac acaccatgct
    13261 tgcccttgtt tttgtcagct ctccttccgg aactttcgga gactgccgaa acaaaaactc
    13321 actggaaatc agttctaaga ccgcatctcc caaaaactct aaacgttcat tgcatctgta
    13381 tttttccatc cgatgctcat tcgtatagga actgtgcatc attgcctgtt caagaaaaga
    13441 aaaatttctg aatcgataac ctatcttctg ttctaactct tttaatttct tatgcatttt
    13501 ctactctttc ccggtgtctt gcaccgtcct ttatttcttt tccatgtaaa acgaaaaagc
    13561 ccacaggggc tttttcattt tacaccaaat tattccactg cttttttgat ctgatcaaca
    13621 gcatcctgaa ctgttgttat tttctccgct tcttcattat cgatctcaat gtcaaactct
    13681 tcttcgattc cca
//
LOCUS       DBMDXF010000031        13558 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1323_length_13558_cov_2.235491, whole genome shotgun sequence.
ACCESSION   DBMDXF010000031 DBMDXF010000000
VERSION     DBMDXF010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13558)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1323_length_13558_cov_2.235491"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(109..594)
                     /locus_tag="ACZPD4_02380"
     CDS             complement(109..594)
                     /locus_tag="ACZPD4_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR1377959.1"
                     /translation="MTKDVLVSISGKHIDIMDDPARGYEVGEDGIEVVTPANYYCRNG
                     KHYILYDEVLEGMAGTIKNKIKITGTDCLEIMKSGVTSSHMVFEKNKKNLTYYKTPYG
                     QMLVGVNTKNMEISVDEDQIGVKVAYELDVNHEPMADCRINMSIVPRNSKGVSMQLDI
                     R"
     gene            complement(599..1426)
                     /gene="murI"
                     /locus_tag="ACZPD4_02385"
     CDS             complement(599..1426)
                     /gene="murI"
                     /locus_tag="ACZPD4_02385"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR1377960.1"
                     /translation="MQMDCKKTAPVGVFDSGVGGLTVAREISRQLPNENIVYFGDTAR
                     VPYGSKSQNTIIRFSEQIIRFLKTKQVKAIVIACNTASALALDAVRDEFDIPIMGVVI
                     PGARAAVETTRNQKVGVIGTDATVQSGMYTKVIQSMAPDIEVIEKACPLFVPLVEEGF
                     KEHVVTEEVIEYYLESMRHTQIDAMILGCTHYPLLRSKIRDYLGEEIKIVNPAYETAM
                     DLKKLLYEKEMANDGTDAQHSVYSFYVSDAAEKFRRFANTVMPFDVPTTNVVNIEEY"
     gene            complement(1501..2511)
                     /gene="pfkA"
                     /locus_tag="ACZPD4_02390"
     CDS             complement(1501..2511)
                     /gene="pfkA"
                     /locus_tag="ACZPD4_02390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1377961.1"
                     /translation="MAENQKEKYLEEIEDQIRTIGVLTSGGDAPGMNAAIRAVVRTAL
                     SRGLKVRGIRRGFHGLLKEEIIDLSARDVSEKIQRGGTFLQTARCKSMRTEEGQQKAA
                     AICKKYGIDGLVVIGGDGSFAGAQKLANLGVNTIGIPGTIDLDIACTDYTIGFDTAVN
                     TAMEAIDKVRDTSTSHERCSIIEVMGRDAGYLALWCGIANGAERILMPEEHDFDEQEI
                     IDDILKNKKRGKKNYIIINAEGIGDSINMAKRIEAATGIETRATILGHMQRGGSPTCK
                     DRVYASIMGSMAVDLLCQGKTNRVVGYKNGEYVDYDIEEALAMKKTVSEYQYEVAKTL
                     AL"
     gene            complement(2602..6075)
                     /locus_tag="ACZPD4_02395"
     CDS             complement(2602..6075)
                     /locus_tag="ACZPD4_02395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1377962.1"
                     /translation="MSFAHLHVHTEYSLLDGSNKIKEYVARVKELGMNSAAITDHGVM
                     YGVIDFYREAKSQGINPILGCEVYVAPNSRFDREVTGGEDRYYHLVLLAENNEGYANL
                     MKIVSKGFVEGYYYKPRVDKELLRKYHSGIIALSACLAGEVSRYLMKGLYDEAKKAAL
                     EYRDIFGKDHFYLELQDHGLPDQGLVNQQLLKMSRETGIELVATNDVHYTYAKDEKAH
                     DILLCIQTGKRLADENRMRYEGGQYYIKSEEEMKSLFPYALQALENTQKIADRCLVEI
                     EFGVTKLPKYDVPDGYTSWEYLRKLCYEGLEKRYAERADELRERLDYELGTIKNMGYV
                     DYFLIVWDFIKYAKDHGIAVGPGRGSAAGSIVSYCLGITTIDPIRYQLLFERFLNPER
                     VSMPDIDVDFCYERRQEVIDYVVRKYGKDRVVQIVTFGTLAARGVIRDVGRVMDMPYA
                     FVDSIAKMIPQEPKMTIEKALKVNPELRKMCEDDEEVKYLIEMAKRLEGLPRHSSMHA
                     AGVVISQKSVDEYVPLSRAADGSVTTQFTMTTLEQLGLLKMDFLGLRTLTVIQNAVLM
                     ARKKDPSVNMDTLDYDDKKVLEYIGTGKTDGIFQLESAGMKSFMKELKPNSLEDMIAG
                     ISLYRPGPMDFIPQYIKGKNDASSISYDCPQLEPILNSTYGCIVYQEQVMQIVRDLAG
                     YSLGRSDLLRRAMSKKKGEEMKRERQIFLYGDEASGIPGCVKNGIDEQVANKIYDEML
                     DFANYAFNKSHAAAYAVVAYQTAWLKYYYPVEYMAALMTSVIDNPSKVAEYIYASRQM
                     GIQILPPDINNGEADFSVDEGNIRYGLSAIKSVGRPVVRAMLEERRTFGPYKNLEDFI
                     TRVSSKEGLNKRTIENLIKAGALDTLGGTRKQFMSIYIQIMDHVNQEKKYSMSGQMSL
                     FDLVGEEEKADFQIRMPDVGEYSKENKLAFEKEVLGVYISGHPLEEHEERWRRMISAT
                     TADFQFDEELNRTKVHDGEKSVVGGMIVDKTVKYTKTNQVMAFLTLEDLLGTVEVVIF
                     PRDYEKNRQWLEINEKVFISGRVSEEDEKASKLICEKVIPFERTKRELWIQFPDKETF
                     LDEEQIVYGYLADSEGEDEVIIYCAKERAVKRLPKNRNIQINEQVLSRLMNHFGESRV
                     KVVEKAIENHF"
     gene            complement(6107..6946)
                     /locus_tag="ACZPD4_02400"
     CDS             complement(6107..6946)
                     /locus_tag="ACZPD4_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvfB family protein"
                     /protein_id="HIR1377963.1"
                     /translation="MKLGEKQVLTVVKIVDFGVYLGNDEERVLLPKKQVPQGIEVGDP
                     IEVFLYKDSSDRMIATTNEPKLTIGKTAVLEVTDVGRIGAFLDWGLEKELLLPFKEQT
                     ERVEKGDRCLVSLYIDKSGRLCATMKVYQLLRTDSPYKKDDMVSGTVYELSRDFGVFV
                     AVDDQYSAMIPKREVYGKMYIGQRIEARVTAVKPDGKLDLSIRGKIPEQMDADADKVF
                     GRIEKNGGMLPFTDKADPERIKSEMGMSKAAFKRAVGRLLKQGKIQIDNEVGKIFAVN
                     KNR"
     gene            complement(6986..8254)
                     /locus_tag="ACZPD4_02405"
     CDS             complement(6986..8254)
                     /locus_tag="ACZPD4_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HIR1377964.1"
                     /translation="MYIADFHIHSRYSYATSKTCTLEYLDLWARRKGIHIVGTGDFTH
                     PAWRAELKEKLAPAEDGLYILKEEYRLYDEADGKKIDAYGRGQKEMIPRFIISGEISS
                     VYKKEGRSRKVHSLLLLPDFEAAERIADRLSQIGNICSDGRPTLRLDCRDLLELALDE
                     CGNSIYIPAHIWTPHFSVFGEFSGFETPDECFGDMTSYVYAMETGLSSDPLMNRRVSV
                     LDDYRLISNSDAHSPGNLGREATLFDVELSYRGIAEAIRTGNGLCGTIEFFPQEGKYH
                     LDGHRKCGVCFTPAETKAHGGVCPVCGRAVTVGVAHRIEEMADRSEEEAKSAAGKEPF
                     ESLIPLKEVIAMANGFAVKGKRTEREYMRLLVQLGPEFEVLRKIPVEQISRTAGEQTG
                     CLVDKLRKGKIKWNSGFDGEYGTIDPGVIQ"
     gene            complement(8420..8965)
                     /locus_tag="ACZPD4_02410"
     CDS             complement(8420..8965)
                     /locus_tag="ACZPD4_02410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1377965.1"
                     /translation="MRDFEQSIIINEKIKLEERFEFRTIFSEEAVQAASVERVCFPPN
                     EACTEEMMKQRALKIRELFFVAVDRKSGEVAGFLNGLATDGDCLKDEFFRNADLHDPT
                     GDNVMILGLAVLPKYRGIGVASELMRRYSGIQRQQGRCALILTCLDAKVEMYKKMGFE
                     DEGISDSSWGGEQWHQMRKGL"
     gene            9281..10048
                     /locus_tag="ACZPD4_02415"
     CDS             9281..10048
                     /locus_tag="ACZPD4_02415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1377966.1"
                     /translation="MIYEIKDTNKISPLFGHWEETLIWSCLQGIMGKIYANDLTAPTA
                     AMAVIGDFTFFAGEPCPELIAYRPPHCTQNFMIAVPQNKLWENMLTQFYGKKAKIISR
                     YATKKEPPIFNKHHLEKIVASLPKQYTLSMIDSHFYKLCKSEAWSKDLVSQFSTYEKY
                     RRLGIGVVISKDGLIISGASSYSRYQNGIEIEIDTRKEYRRNGFACICAAKLILECLK
                     RNLYPSWDAHNKASAALAEKLGYHYSHAYTALEIWDY"
     gene            complement(10247..11539)
                     /gene="guaA"
                     /locus_tag="ACZPD4_02420"
     CDS             complement(10247..11539)
                     /gene="guaA"
                     /locus_tag="ACZPD4_02420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1377967.1"
                     /translation="MKQDMIVILDLGSTENTVLAREIRELGVYSEIHPHDITEEEIKA
                     LDNVKGIILNGGENRVVDGKEVEVRPELYTWGYPVISVDYPASRCDVRFDSLPDQETL
                     KKFVFDECKAEANWNMKNFIEDQVELIRRQVGDRKVLLALSGGVDSSVVAAMLIKAIG
                     QQLVCVHVNHGLMRKNESESVVEVFRNQLHANLIYVDATERFLGKLENVSDPEEKRKI
                     IGGEFIRVFEEEARKLEGIDFLGQGTIYPDIIESGTKTAKMVKSHHNVGGLPEDLQFE
                     LVEPLKQLFKDEVRACGIELGLPAEMVYRQPFPGPGLGVRCLGAITRDRLEAVRESDA
                     ILREEFAKAGLDKKVWQYFTVVPDFKSVGMKNHARCFEYMVIIRAINTIDAMTASIEH
                     VDWEILDRITNRILAEVENVNRVCYDMSPKPPATIEFE"
     gene            complement(11599..12579)
                     /locus_tag="ACZPD4_02425"
     CDS             complement(11599..12579)
                     /locus_tag="ACZPD4_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1377968.1"
                     /translation="MDRFIREIAITEIDGVSIGHAQDNEAKTGASVLYFKRGAQAGCD
                     ISGGGPASRETPLTDSMTADNPLNAVVLSGGSAYGLAASDGVMRCLEEQGIGYDTGFS
                     KIPLVCQSCIFDLGYGRSDIRPDADMGYEACRKAMEGCDVSMGNVGAGTGASVGKLFG
                     MERATKSGLGIYALQAGELKIAAVVVVNAFGDVFDPENGQKIAGLMDAERTKFVDLEE
                     AFVEMMTTPQDLFHTNTTIGCIVCNARFDKAKLNKIASMARNAYARCINPVGTMADGD
                     SIYACSVGEVESDVNVAGTLAARAMQQAIKRAVTASAVDDREYLKYCLRN"
     gene            complement(12617..12931)
                     /locus_tag="ACZPD4_02430"
                     /pseudo
     CDS             complement(12617..12931)
                     /locus_tag="ACZPD4_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014019.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
BASE COUNT         3465 a         3719 c         2310 g         4064 t
ORIGIN      
        1 cctgaaaaga aaaaaggatt tttcaaaaaa taattaaaaa gcaaaaaaac agacgtcacg
       61 aaggaatatt ccttttctgc tgcgtctgtt ttttgttatt tttctttcct accggatatc
      121 cagctgcatg gagacccctt tactgtttcg cggcacaatg ctcatattaa tgcgacagtc
      181 tgccataggt tcgtgattta catctaattc ataagcgact ttaacgccga tctgatcttc
      241 gtctacgctg atctccatat tttttgtatt tacgcctacc agcatctgac cataaggcgt
      301 cttataatat gtcagatttt ttttattttt ttcaaatacc atatgagagg aagtaactcc
      361 gcttttcata atttcaagac agtccgttcc cgtgatcttg atcttatttt taatcgtgcc
      421 tgccattcct tcaagaactt catcatagag aatataatgt tttccattcc ggcagtaata
      481 atttgccgga gtcacaactt caatcccatc ttctccgact tcatatcccc ttgcaggatc
      541 gtccataata tcgatatgtt tccccgaaat gctgactaat acatcttttg tcatcaatct
      601 aatactcctc aatattcact acattcgttg tcggcacgtc aaacggcatt actgtatttg
      661 cgaatctgcg gaatttttcc gccgcatcac ttacataaaa ggagtataca ctgtgctgtg
      721 catctgtccc gtcatttgcc atttcttttt catacaaaag ctttttcaga tccatagccg
      781 tttcatatgc cggattcaca atcttgatct cctctccgag ataatcgcgg atcttagatc
      841 taagaagagg gtagtgggtg caccccaaaa tcatagcgtc aatctgtgtg tgccgcattg
      901 actccaaata atattcgatc acttcttctg taacaacatg ttccttaaac ccttcttcca
      961 ccagcggaac aaacagcgga catgcctttt cgatcacttc aatatccgga gccatgcttt
     1021 ggatgacttt cgtgtacatt ccgctctgta cagtcgcatc cgttcctata actccgactt
     1081 tctgattccg ggttgtctct acagccgctc ttgcgcccgg gatcacaaca cccatgatag
     1141 ggatatcaaa ttcatctctc acggcatcca gcgccaaagc gctcgccgta ttacaggcaa
     1201 taacgatcgc ttttacctgc tttgttttca aaaagcgtat aatctgctct gaaaaacgga
     1261 tgattgtatt ctgcgactta cttccgtacg gaactcttgc cgtgtctcca aagtatacga
     1321 tattttcatt cggaagctgc cgggaaatct ccctcgcaac cgtaagtccg cccactccgg
     1381 aatcaaaaac tccgacaggc gcggtctttt tgcagtccat ttgcatgatt ccctccatcg
     1441 tgcaaacagc cggtataaat ttataccggc atgtttttag aaatatatgt ctgttttttc
     1501 ttacagtgca agtgtttttg ccacttcgta ctgatactcg gatactgttt ttttcatagc
     1561 cagagcttct tcgatatcat agtctacata ctcgccgttc ttatatccta ctacacggtt
     1621 tgtttttccc tgacaaagaa gatcaactgc catagatccc atgatcgaag catatactct
     1681 gtctttacat gtcgggcttc ctccacgctg catatgtccg agaatagtcg ctctcgtctc
     1741 aatacccgtc gccgcctcga ttctctttgc catgttgatc gaatcgccga taccttccgc
     1801 attgatgata atgtaatttt tcttaccgcg ttttttattt ttcaaaatat catcaatgat
     1861 ctcctgctcg tcaaagtcat gctcttccgg cataaggata cgttctgctc cattggcaat
     1921 accgcaccac aaagcaagat atcccgcgtc acgccccata acttcgatga tgctgcatct
     1981 ttcatgagac gtcgaagtat cacgtacttt atcgatcgct tccatcgccg tatttacagc
     2041 agtatcaaat ccgatcgtgt aatctgtgca ggcaatatca agatcgatcg ttcccggaat
     2101 gccgatcgta tttacgccga ggttcgcaag tttctgagct ccggcaaaag aaccgtctcc
     2161 tccgataact acaagtccgt cgataccgta ttttttacag attgcagccg ctttctgctg
     2221 tccttcttcc gtacgcatag atttacaacg cgctgtctga aggaacgtac ctccgcgctg
     2281 gatcttctca gacacatcgc gcgctgaaag atcaatgatc tcctccttca aaagtccgtg
     2341 aaatcctctt ctgataccac gcactttcag tcctcttgaa agcgccgttc ttacgactgc
     2401 acgaatagcc gcgttcatac ccggcgcatc accgccgctt gtaagaactc cgattgtacg
     2461 gatctgatct tcaatctcct ctaagtactt ttctttttga ttctctgcca tgatttttcc
     2521 acctttctgc aaagtgtcgc tattaataat atttattcat tgtaattttt ttaacaatag
     2581 tcgaaaatta ttctacatta tttagaagtg gttttcaata gctttttcaa ccacttttac
     2641 ccgactttca ccgaaatggt tcattaacct gcttaaaacc tgctcattga tctgaatatt
     2701 ccggtttttc ggcagtcgtt tcactgcccg ttcttttgcg caatatataa taacctcgtc
     2761 ttcgccttcc gaatctgcca ggtatccgta aacgatctgt tcttcatcaa gaaacgtctc
     2821 tttatccgga aattgaatcc acaattctcg ttttgtcctc tcaaagggga tcactttctc
     2881 acagatcagc ttacttgcct tctcgtcttc ttccgaaacc cttccgctga taaacacttt
     2941 ctcgtttatt tcaagccatt gacgattttt ctcgtaatct ctcggaaaaa taacaacttc
     3001 cacagtgccg agcaaatctt ccagcgtaag gaacgccatc acctgattcg tctttgtgta
     3061 cttgaccgtc ttgtcaacga tcatccctcc gacaaccgac ttttctccgt catgcacctt
     3121 cgttctattc agttcctcgt caaactgaaa atctgccgtt gtagccgaaa tcatccgccg
     3181 ccatctctcc tcgtgctcct caagcggatg accgcttata taaacgccga gcacctcttt
     3241 ttcaaacgca agcttatttt cttttgaata ctcgcctaca tcaggcattc tgatctgaaa
     3301 atccgccttt tcttcttctc ccacaagatc aaacaaactc atttgaccgg acatggaata
     3361 ttttttctcc tgattcacat gatccatgat ctgaatataa atactcatga attgttttcg
     3421 ggttccgcct aaagtatcca acgcccctgc tttgatcaga ttttcaatag ttcgcttgtt
     3481 cagtccttct ttcgatgaaa cacgggtaat aaaatcttca agatttttat atggaccaaa
     3541 tgtcctgcgt tcttcgagca tcgccctaac aaccggtctc ccgacacttt tgatcgcaga
     3601 cagcccgtat cttatattcc cttcgtcaac cgaaaaatcc gcttctccgt tattaatgtc
     3661 aggaggaagg atctgaatgc ccatctgccg gcttgcataa atatattcag ctactttcga
     3721 cggattgtct atcaccgatg tcataagagc cgccatatat tcaacaggat aataatattt
     3781 cagccacgca gtctgatatg ccacaaccgc atatgcggcc gcatgcgact tattaaacgc
     3841 atagtttgca aagtcaagca tctcatcata aatcttattc gcaacctgct cgtcaattcc
     3901 gttttttaca catcccggaa taccggaagc ctcgtcaccg tacaggaaaa tctgccgttc
     3961 ccgtttcatc tcttcgcctt ttttctttga catggcgcgc ctcaaaagat cgcttcttcc
     4021 gagggaatat cccgcaaggt cgcgaacgat ctgcataacc tgttcctgat aaacgataca
     4081 gccgtatgtc gaattcagga tcggctctaa ctgcgggcag tcatatgaaa ttgaagaggc
     4141 gtcgtttttc cctttgatgt actgcggaat aaaatccatc ggaccggggc gatatagaga
     4201 gattccggcg atcatatctt ccagactgtt cggtttcagc tctttcataa agcttttcat
     4261 tcccgcgctt tccaactgga agatgccgtc tgtttttccg gtcccgatat attcaagcac
     4321 ttttttatca tcataatcca gcgtgtccat atttaccgaa ggatcttttt ttctcgccat
     4381 aagaaccgca ttttgtataa ccgtcaacgt acgaagtccc agaaaatcca ttttcaaaag
     4441 tccgagctgt tccagtgtcg tcatcgtaaa ctgggtcgtc accgagccgt ccgccgctct
     4501 cgacaacggc acatattcgt caacagactt ctgactgatc acgactccgg ccgcatgcat
     4561 agaactatgt ctcggaagtc cttccagtct tttcgccatc tcgataagat attttacttc
     4621 ctcatcatct tcacacatct tgcgcagttc aggatttacc ttcaacgctt tttcaatcgt
     4681 catcttcggc tcctgcggta tcatcttcgc aatcgaatcg acaaaagcat acggcatatc
     4741 catcacacgc cccacatctc gaatcacacc tcttgccgcc agcgttccga atgtcacgat
     4801 ctgaacaact ctgtcttttc cgtattttct cacaacatag tcgatcactt cctgtctgcg
     4861 ttcataacaa aagtcaacgt cgatatcggg catggaaaca cgttcgggat tcaaaaaacg
     4921 ctcgaacaaa agctgatatc tgatcggatc gatcgtcgta attcccaggc agtaggaaac
     4981 aatacttccc gctgcagacc cccggcccgg tccgactgcg atcccatgat ctttcgcata
     5041 ttttataaaa tcccacacga tcaggaaata gtctacatac cccatatttt tgatcgtgcc
     5101 caattcataa tcaagccgtt cccgaagttc atccgcccgt tctgcatagc gtttctcaag
     5161 tccttcgtaa cacagtttcc gtaagtattc ccacgatgta tatccgtccg ggacgtcata
     5221 tttgggaagc tttgttaccc cgaactcaat ctccactaaa cagcggtccg caatcttctg
     5281 cgtattttca agcgcctgaa gcgcatacgg aaaaagactt ttcatctctt cttcagactt
     5341 gatgtaatac tgtccgcctt catatctcat cctgttctca tccgccagcc ttttccctgt
     5401 ctggatacac aaaaggatat catgcgcttt ttcgtctttc gcataagtat aatgcacatc
     5461 attcgtcgcc acaagttcga tccccgtttc ccgggacatt ttcaaaagct gttgatttac
     5521 aagaccctga tcaggcagac cgtgatcctg cagttctaaa taaaaatgat cttttccaaa
     5581 gatatctctg tactccaacg cagctttctt cgcttcgtca tataagcctt tcatcagata
     5641 tctcgacacc tcgcctgcca ggcaggcgct cagcgcgata atgccgctgt gatattttct
     5701 gagaagttct ttatctacac ggggtttgta atagtatccc tcgacaaagc cttttgatac
     5761 gattttcata agattggcat aaccttcgtt attttccgca agaagtacaa ggtgataata
     5821 cctgtcttct cctccggtca cttctctgtc aaaacgggaa ttcggcgcga cataaacttc
     5881 acaccccaag atcggattaa ttccctgact tttcgcctct ctgtaaaaat caatcactcc
     5941 gtacattact ccgtgatcgg tgatcgccgc actgttcatc ccaagctctt tcactcttgc
     6001 aacatattct tttatcttgt tagaaccatc cagaagactg tattctgtat ggacatgcaa
     6061 atgtgcaaaa ctcatctttc acctcgtaag ccgacccgca aaaggtctat ctgttcttgt
     6121 ttaccgcaaa aatctttccc acctcgttgt cgatctgtat ctttccttgt ttcagaagtc
     6181 ttccgaccgc acgtttaaac gccgctttac tcatccccat ctccgactta atcctctccg
     6241 gatccgcctt atctgtaaac ggaagcatgc cgccattctt ttcaatacgt ccaaacacct
     6301 tatccgcatc cgcatccatc tgttccggta tcttcccgcg tatgctcaga tcaagctttc
     6361 cgtccggctt cacagccgtc accctcgctt caatgcgctg accgatatac atcttcccgt
     6421 aaacttcccg cttcgggatc atcgccgaat actgatcgtc cacagcgaca aacacgccga
     6481 aatcccgact taattcatac actgttccgc tgaccatatc gtcttttttg tacggtgaat
     6541 cagtacgaag aagctgatac actttcatcg tcgcacacag tctgccgctc ttatctatat
     6601 aaagactaac gagacatcga tcgccttttt ctactctttc cgtctgttct ttgaacggaa
     6661 gcagaagttc tttttccaat ccccaatcca aaaatgctcc gatccttcca acatccgtca
     6721 cttcaagcac agccgtcttt ccgatcgtaa gcttcggttc attcgtagtt gctatcatac
     6781 gatctgatga atctttatat aaaaacacct ctatcggatc tccgacttct atcccttgcg
     6841 gcacctgctt tttaggcaga agcaccctct cttcgtcgtt gcccagatat acgccgaaat
     6901 caacgatctt cacaacagtc agaacttgtt tttctcccag tttcatacat tttctccttt
     6961 attgttctga aacaaaactt tctatttatt gtatcacacc cggatcgatt gttccatact
     7021 ctccgtcaaa tccggagttc cattttatct tccctttccg aagtttgtca accagacatc
     7081 ccgtctgttc ccctgccgtc cggctgatct gttctacagg tatctttcgc agcacttcaa
     7141 attcagggcc aagctgcaca agaagccgca tatactctcg ttctgtacgt ttccctttca
     7201 cagcaaatcc atttgccatg gcaatcactt ccttaagcgg gatcagactc tcaaacggtt
     7261 ctttccccgc cgcagacttc gcctcctctt ccgatctgtc tgccatctcc tcgattctgt
     7321 gagccactcc taccgtcacc gcgcgcccgc atacaggaca tactcctcca tgtgctttcg
     7381 tctccgcagg agtaaaacaa actccgcatt ttcgatgtcc atccaaatga tactttcctt
     7441 cctgagggaa aaattcaatc gttccgcaca aaccgtttcc cgtccgaatc gcctctgcaa
     7501 tgcctctgta agaaagttcc acatcaaaaa gagtcgcttc ccgacctaaa tttcccggag
     7561 aatgagcgtc cgaattagaa ataagccgat aatcatccaa tacagatact cttcggttca
     7621 tcaacggatc tgacgaaagt cccgtctcca tagcatatac ataggaagtc atatcgccga
     7681 aacactcatc gggcgtttca aatcccgaaa actctccgaa taccgaaaaa tgcggcgtcc
     7741 atatatgagc cggaatataa atcgaattcc cacactcgtc aagcgccagt tcaagaaggt
     7801 cccgacagtc aagccgaagt gtcggccgac cgtccgaaca gatatttcca atctgtgaaa
     7861 gacgatccgc gatccgctct gccgcctcaa aatccggaag gagaagtaga ctgtgcacct
     7921 tccgggaacg tccttctttt ttatacaccg aactgatttc cccacttata ataaaacgcg
     7981 gaatcatctc tttttgtcca cgcccgtatg cgtcgatttt tttcccgtcc gcttcatcat
     8041 aaagtctgta ttcttctttc aatatatata atccatcttc cgcaggagcc agcttttctt
     8101 ttaactccgc ccgccatgcc ggatgtgtaa aatcacctgt cccaacaata tgaattcctt
     8161 ttctccttgc ccataaatca agatattcca gcgtacatgt cttgctcgtc gcataagaat
     8221 agcgggaatg gatatgaaaa tctgcaatat acattttatc tccccgatct gattttgcgg
     8281 tattttccgc attttaggta aatagttcag ccatacagcg tccgtctatg agtaaaacag
     8341 tggcatcagc cacgataagc acacagtgcg gttttacgaa tagcgcgggc tgaactgtta
     8401 ccattttaat tcatttattt tataatccct tccgcatttg atgccattgt tctccgcccc
     8461 aagatgaatc cgatatccct tcatcttcaa agcccatctt tttgtacatc tcgacttttg
     8521 cgtcaagaca tgtcaggatc aacgcacatc tcccctgctg cctttgtatc ccggaatacc
     8581 gccgcatcag ttctgatgcc actccgattc cccggtactt cggcaaaact gcaagaccga
     8641 gtatcatcac gttatcacct gtcggatcat gcaagtctgc attcctgaaa aattcatctt
     8701 tcaaacaatc cccgtccgtt gccagaccat tcaaaaaacc tgccacttca ccgctttttc
     8761 tgtcaaccgc cacaaaaaac aattcccgaa tcttaagagc ccgctgcttc atcatctctt
     8821 cggtacacgc ctcgttagga gggaaacata ctctttcgac agacgcagcc tgcacagctt
     8881 cctcagagaa tatagttcgg aactcgaatc tttcttcaag ctttattttt tcatttatta
     8941 taatactttg ctcaaaatct ctcatgtcat acactcctgc actctctgcc gtcatataac
     9001 acagcagcag atcttctttt taaattgtag tatactgtct gaggaaattc aaggaaaaga
     9061 actctcttcc tccgttcctg tttgcttatt tacaccaaac atattaaact atactcaaac
     9121 ctgccactac ataattaccg gaagcactaa acgacacatt tcagtattgt gaaaagccac
     9181 cgttccagca aacagctcct aataccactg ttccacctga cagtctgccc acaccttata
     9241 atgatccgct ccttataata atgagaaaca agaggaaaca atgatatacg aaataaaaga
     9301 cacaaacaaa atttcacctt tatttggaca ctgggaagaa acccttatct ggtcatgcct
     9361 gcagggaatc atggggaaaa tatatgcaaa cgacctcacc gcccctactg cggctatggc
     9421 cgtgatcggg gatttcacct ttttcgcagg cgaaccatgc ccggaactga tcgcttatag
     9481 accgccccac tgtactcaaa actttatgat cgccgttcct caaaataaac tttgggaaaa
     9541 catgctcact caattttacg gtaaaaaagc aaaaataatt tctcgctacg caacaaaaaa
     9601 ggaacctcct attttcaata aacatcattt agaaaaaatc gtcgcttccc tgccaaaaca
     9661 atatacactt tccatgatcg actcgcactt ttacaaatta tgcaaatcag aagcatggag
     9721 caaagatctc gtatcacaat tttcaactta cgaaaaatac cgccgtctcg gaataggcgt
     9781 tgtaatatcc aaagacgggc tgatcatatc cggtgcttca tcctactcca gatatcaaaa
     9841 cggcatcgaa atcgaaattg acacacgaaa agaataccgg agaaacggct ttgcctgcat
     9901 atgcgccgca aaactgatct tagaatgctt aaagcggaac ttatatccca gttgggatgc
     9961 acataacaaa gcatccgccg cactggcaga aaaattaggg tatcattaca gtcacgctta
    10021 tacggcgttg gaaatatggg attattaaga ttattgtaga ttcggtttct aatcctgccg
    10081 gcgacgtgga gaaaagagca atggataata tctaaaattc ctgtttgaac atcgttcaaa
    10141 tgaacgtatg tcggatgaag aatttcaaaa ttattgtcat ttgattgtca ctagacatag
    10201 agagccaaga aagcccgcaa aataggcatt tcttggctct aatatattat tcgaattcaa
    10261 tcgtcgccgg gggcttcggg ctcatatcat agcacacgcg gtttacgttc tcgacctctg
    10321 ccaggattct gttagtgatt ctgtcgagaa tctcccagtc tacgtgctcg atgctggctg
    10381 tcatcgcgtc gatggtattg attgcacgga tgattaccat gtactcaaag catcttgcat
    10441 gattcttcat tcccacagac ttgaaatcgg gtactaccgt aaaatactgc catactttct
    10501 tgtccagtcc tgcctttgcg aactcctcgc gcaggatagc atctgactca cggacagctt
    10561 ccaggcgatc tcttgtgata gcgccgaggc agcgaacacc aagtcccgga cccgggaacg
    10621 gctgacggta aaccatttca gcaggcaggc caagctcaat accgcaggca cgaacttcgt
    10681 ccttgaagag ctgctttaac ggctctacca attcaaactg caaatcttcc ggaagtcctc
    10741 ccacattgtg gtgagatttt accatctttg cagtcttggt tccgctctcg ataatgtccg
    10801 gataaatcgt tccctgtcca aggaaatcga ttccttccag ttttctcgct tcttcctcga
    10861 aaacacggat aaattctccg ccgataatct ttctcttctc ttccggatca gatacatttt
    10921 ccagctttcc aaggaaacgc tctgtagcgt ctacataaat cagatttgca tgaagctggt
    10981 tgcggaatac ctctacaacg ctttcagatt catttttccg catcagacca tgatttacat
    11041 gtacgcagac gagctgctgt cctatcgcct taatcagcat cgctgcaact acggaagaat
    11101 ctacgccgcc tgacagcgcc aaaagaacct ttctgtctcc tacctgtctg cggatcagct
    11161 ctacctgatc ttcaataaag ttcttcatgt tccagtttgc ttctgcctta cattcatcaa
    11221 atacgaactt tttcaacgtc tcctgatccg gcaggctgtc aaaacgaacg tcgcatctgg
    11281 atgcaggata gtctacggaa attaccggat agccccatgt ataaagctct ggtcttactt
    11341 ccacttcttt tccatctacc acacggtttt ctccgccatt taaaataatt ccttttacat
    11401 tgtcaagagc ctttatttct tcctctgtaa tatcatgagg atgaatctcg ctgtatacgc
    11461 caagttcacg aatctcccgg gcgagtaccg tattctctgt acttcccaga tccaaaataa
    11521 caatcatatc ctgcttcatt tgtctctcct tttaactggt cagattattt ccaacatttt
    11581 tattttactg attttccttt agtttcgcaa gcaatatttc aaatattctc tatcatccac
    11641 tgcggacgct gtcacagctc tttttatcgc ctgctgcatc gcccttgccg ccagtgttcc
    11701 ggctacattt acatcgcttt ctacctctcc cacactgcag gcatagatgc tgtctccgtc
    11761 agccatagtt ccaaccggat tgatgcatct cgcatacgca tttcttgcca tagaagcaat
    11821 tttattcagc tttgccttat caaaacgtgc attgcacacg atgcagccga tcgtcgtatt
    11881 tgtatgaaat aaatcctgag gcgtcgtcat catttctaca aacgcctctt ccaaatccac
    11941 aaatttcgtt ctttccgcat ccataaggcc tgctatcttt tgtccgttct ccggatcaaa
    12001 aacatctccg aatgcattaa cgactacgac agccgctatt ttcagctccc ctgcctgaag
    12061 cgcatagata cccagaccgg actttgtcgc ccgttccatt ccaaacagct ttcctacgct
    12121 ggctcccgtt cctgctccga catttcccat cgatacatcg cagccttcca tcgcctttct
    12181 acaggcctca tatcccatat ccgcatccgg acggatatcg cttcttccgt atcccaagtc
    12241 aaaaatgcag gactgacata caagaggtat tttggaaaat cccgtgtcgt atccaatccc
    12301 ctgctcctcc agacagcgca tcacaccgtc cgaagccgcc agaccatatg cgcttcctcc
    12361 tgacaacacg acagcgttca gcggattatc tgccgtcatg ctgtctgtaa gcggcgtttc
    12421 tctcgacgcc ggacctcccc cgctgatgtc gcagcctgcc tgtgcgcccc gtttaaagta
    12481 caatacgctt gcgcctgtct ttgcttcatt atcctgcgca tgtcctatgg atacgccatc
    12541 tatctctgta attgcgattt cccttataaa tctgtccatc aaatttccct ctcacccttt
    12601 ctgttcacac ccgaagctat tcggcaacca ctgccgttac cataagcatt ccgttacctg
    12661 caccgagcac ctcataatcg atatcaacgc ctacaaccgc attggccccc aaatctgccg
    12721 cacgctgttc catctccttc agggcgttct gtctcgccta aacaagctct tcctcatagc
    12781 ttcctgatct tccgccgaaa aagttgctga aagaagccgc aacatcgcgc acgaaattta
    12841 cgccagatat gacttctcca aatacaattc ccttatattc cgtaattctt cttccttcta
    12901 cagacactgt cgttgtcact atcattccca ttcctccgtt tcttcctttt atagggtact
    12961 gtgaaagtag aagctttctg ttgattgttg caaaatgcgt tacaaattta ttatttatat
    13021 catagcaaaa atcattttaa gatacaatcg tttcctggca ggaaatcctg cctggttact
    13081 aaaattaaat tcccccaaat ttttacgaaa tagaggggtg tctatctttc aatttcttgt
    13141 gtgatacttt accgcacatg aacattggca gagggaatga aacaaaaaga cattgcattt
    13201 cagttaggta tctctgaagc ctatgttact caactcaaaa aagttatagc attttttctt
    13261 actagaatta gagccatttt agggccaaaa accataaaac aaccccggaa atgcccattt
    13321 tatcagcatt tccggggttc tgtccgttat tcaaactcga caattactaa tcaattttgt
    13381 ttagagatta ttctctgtcg tgtttacagt tcttttgatt atttgattta tccatattat
    13441 cctgcctata caggctaatg ttatcatttt gttatcggtg ttgataacac ttgctctgta
    13501 actgtggata atagcactat attctatttc agatcataag cgattttgct taaaaaat
//
LOCUS       DBMDXF010000032        13478 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1330_length_13478_cov_2.151529, whole genome shotgun sequence.
ACCESSION   DBMDXF010000032 DBMDXF010000000
VERSION     DBMDXF010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13478)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1330_length_13478_cov_2.151529"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(198..1343)
                     /locus_tag="ACZPD4_02435"
     CDS             complement(198..1343)
                     /locus_tag="ACZPD4_02435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1377969.1"
                     /translation="MKKPHTKARGILFAAAGMFLIPGSVSEVSAAERKDEQPFIQASG
                     IESVLEKCYETEVKDNINLYLVPTEEGEYLNMAFSDTKEFTYIRNAPDDSGDWVGKLY
                     KDSAAQVLEYLDGWTKIRSGSAEGYVPEDALFTGEEAQARAQEYEKDTVTVTAYVLNV
                     REGCGTDSKILTQIKKGEVYETVGEATGGWYPVKVGDKSGWVSGDYVIAETSYSYGET
                     KEEEEKRIAEEEAQEKMAASVVCGADGRPGTRPVGAVSQGRVNTPSQAPGTPGQAVID
                     YACQFIGNPYVWGGTSLTNGADCSGFVQSVYAHFGVSLPRTTYDMVNSGYAVSYEEAL
                     PGDLILYDGHVGLYMGDGTIVNAMNEADGIGICSATYTDIIAVRRVL"
     gene            1726..2121
                     /locus_tag="ACZPD4_02440"
     CDS             1726..2121
                     /locus_tag="ACZPD4_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1377970.1"
                     /translation="MAALKYSRQRESIKHYLMTTKEHPTADTVYMHVKKEFPNISLGT
                     VYRNLNLLTDIGEAIKISTPDGGDRFDGRIEPHNHFLCEECGRLLDLDLDMKSIEEVN
                     CLAGKNFDGAITSSSTLFFGKCGDCIKKS"
     gene            2162..2704
                     /locus_tag="ACZPD4_02445"
     CDS             2162..2704
                     /locus_tag="ACZPD4_02445"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HIR1377971.1"
                     /translation="MKKFVCTVCGYVFEGEAAPAECPVCHAGADKFKEQTGEREWAAE
                     HVIGVAQGVSEDILADLRANFEGECTEVGMYLAMARVAHREGYPEIGLYWEKAAWEEA
                     EHASKFAELLGEVVTDSTKKNLEMRVDAENGATAGKFDLAKRAKAANLDAIHDTVHEM
                     ARDEARHGKAFEGLLKRYFG"
     gene            2915..3814
                     /locus_tag="ACZPD4_02450"
     CDS             2915..3814
                     /locus_tag="ACZPD4_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1377972.1"
                     /translation="MRNFIGVDLGGTNIRAAIVSEDGTIKCMKKSESHPERGAEAVME
                     TMISLIESLDGYEECEGIGMGIPGPIDTINGKIIVSTNLPKLIGFPIAEYIENHFHKP
                     TYMDNDVKVAALGEAVQGAGKDYPIVYYVTISTGVGGALVIDQKVIGGQNGHAGEIGN
                     ICIDRNREKYNILNVGAVENEASGTAVTRKGKAVFGDKINHAGDVFDLARSGDEQALK
                     IVDDMAYDLAMMFCAIGHIIDPHVFVVGGGVMKGKDVFFEKMESYYRSMIHVGMQPVV
                     FKEALLDEPGIIGAAMLPMTHLK"
     gene            complement(3900..4148)
                     /locus_tag="ACZPD4_02455"
     CDS             complement(3900..4148)
                     /locus_tag="ACZPD4_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR1377973.1"
                     /translation="MNYTYIVRCSDGSLYTGWTNNLEKRIKDHNAGRGAKYTKARRPV
                     VLVYKEEFPTKQEAMKREWEIKRLSRKEKLSMIENSKS"
     gene            complement(4230..4970)
                     /locus_tag="ACZPD4_02460"
     CDS             complement(4230..4970)
                     /locus_tag="ACZPD4_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1377974.1"
                     /translation="MNKNEKMSFLKGKGAAIGIVVCFMAVLAVVGTYTVGNYKKDMKE
                     QMAKAEKEAEQFTEDNTKETTTDEILLPEVEETDENEQESREDVSDTTGTEEGVQQTG
                     SAASNLWFSEESVLTWPASGAVINGYSMDETVFFQTLEQYQYNPAMIIAGEAGETINA
                     SAAGTVTAIEESAQTGLTVTLDMGNGYSAVYGQLKDVQVKTGDYVAAGSAIGSLNEPT
                     KYYSVEGTNLYFQVLKDGEPVDPMNFME"
     gene            complement(5069..6109)
                     /locus_tag="ACZPD4_02465"
     CDS             complement(5069..6109)
                     /locus_tag="ACZPD4_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1377975.1"
                     /translation="MKRYTIEQKFKSAAAFLVILILFPYIVSVFVNGVDPEETTNENF
                     YIRVKASEEEEADGIAEVEWNEYLTGVLAKDVPADTETEALKAQAVLVRTQISRALQR
                     STQAEETENQKSDRTGNVSDVILEIDYLSQKEMQDQWPADEYKKRYEKYRNAIEATEN
                     EVLFYNDTYAWTPFHQSNTGMSRSAEEVLGSSEFPYIKVKECPADKESEEQVGVFTFS
                     YDQIQKKCHHFLAAETDGEKAAAGYSFKDFEILEHDSAGYVGKLRIGETICTGDQFRD
                     ALSLPSSSFSFAESGKKIKIITAGNGHGMGLSCWTANKMAKDGKTYKEILEFFYEGTK
                     TEKDLNFIAKNK"
     gene            complement(6170..6808)
                     /locus_tag="ACZPD4_02470"
     CDS             complement(6170..6808)
                     /locus_tag="ACZPD4_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1377976.1"
                     /translation="MRNMYDYAYEYEYQYHPERAGEMFAVVFASLWLAIMIGMLVFML
                     GSYLLHSFGLYTIAKRMGKKDAWMAFVPFARTYYHGNMAGPIRLKNTTIKNPGLWKLL
                     FPIIYSGAIGIYVLVLVFGIGMTLAGSAVNQNVTGAGMSVLAFFFMYFVVIIVSILFG
                     AVQIALNVLVNKQIYENFAEGNMPVIHAVLSSLVPLYEAICLFIMRKRPFVR"
     gene            complement(6854..9325)
                     /locus_tag="ACZPD4_02475"
     CDS             complement(6854..9325)
                     /locus_tag="ACZPD4_02475"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1377977.1"
                     /translation="MHNKGFEKETFKETVRENIKTLYRKTIDEATPQQIFQAVSYAVK
                     DVIIDDWIATQKAYDEADAKTVYYMSMEFLMGRALGNNLINLTAYKEVKEALEEMEID
                     LNVIEDQEPDAALGNGGLGRLAACFLDSLATLNYPAYGCGIRYRYGMFKQKIKDGYQV
                     EVPDNWLKEGNPFEIRREEYAKEVRFGGNIRFENDPETGKAKFVQENYESVLAIPYDM
                     PIVGYGNHVVNTLRVWDAKAITDFKLDEFDRGNYHKAVEQENLAKLIVDVLYPNDNHY
                     SGKELRLKQQYFFISASLQALLEKYKKKHSDVRKLHEKVIIQMNDTHPTVAVPELMRL
                     LIDQEGLSWEEAWEVTSKTCAYTNHTIMAEALEKWPIDLFSRLLPRIYQIVQEIDRRF
                     LIKVNEMYPGNEHKVKKMAILRDGQVRMANMAIIAGFSVNGVAKLHTDILKTQQLRDF
                     YEMMPEKFNNKTNGITQRRFLAHGNPLLADWITDKIGNGWITDLSQIEKLRPFADDEK
                     ARREFMQIKYENKVRLAKYIKEHNGIEVDPRSIFDVQVKRLHEYKRQLLNILHIMYLY
                     NQIKEHPEMSFYPRTFIFGAKAAAGYLRAKETIKLINSVADVVNNDRSINGKLKVVFI
                     EDYRVSNAEIIFAAADVSEQISTASKEASGTGNMKFMLNGAPTLGTMDGANVEIVQEV
                     GAENAFIFGLSSEEVINYENNGGYNPTDIYFNDWELKRVIDQLMDGTYANGDHEMYKN
                     LYNSLLNTQCTDRADTYFILKDFRSYAEAQKRVEEAYRDQQRWSRMAMMNTICSGKFS
                     SDRTIEEYVSDIWKLEKVDVAPLEE"
     gene            complement(9418..12567)
                     /gene="ileS"
                     /locus_tag="ACZPD4_02480"
     CDS             complement(9418..12567)
                     /gene="ileS"
                     /locus_tag="ACZPD4_02480"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1377978.1"
                     /translation="MYKKVSTDMNFVDREKQIEKFWDDNHIFEKSMEEREGCPDYIFY
                     DGPPTANGKPHIGHVLTRVIKDMIPRYRTMKGSMVPRKAGWDTHGLPVELEVEKLLGL
                     DGKDQIEEYGLEPFIDKCKESVWKYKGMWEDFSGTVGFWADMDDPYVTYHNSFIESEW
                     WALKQIWDKGLLYKGFKIVPYCPRCGTPLSAQEVAQGYKDVKERSAIVRFKVKEEDAY
                     ILAWTTTPWTLPSNLALCVNPKEDYAKVKAADGNIYYMACALLDTVLGRLAEEGKAAY
                     EVLETYKGTDLEGKEYEPLYQCAADCAQKQNKKAFYVVCDEYVTLTDGTGVVHIAPAF
                     GEDDANVGRKYDLPFVQLVDEKGNMAEETPFAGLFVKKADPEVLKDLDARGLLYDAPK
                     FEHSYPHCWRCDTPLIYYARESWFIKMTAVKEDLIANNNTINWIPESIGKGRFGDWLE
                     NVQDWGISRNRYWGTPLNIWECECGHMHSVGSIEELKEMSDNCPDDIELHRPYIDAVT
                     IKCPKCGKEMHRVPEVIDCWFDSGSMPFAQHHYPFENKELFERQFPADFISEAVDQTR
                     GWFYSLLAISTLLFNKAPYKNVIVLGHVQDENGQKMSKSKGNAVDPFDALEKYGADAI
                     RWYFYVNSAPWLPNRFHGKAVVEGQRKFMGTLWNTYAFFVLYANIDQFDATKYELEYN
                     KLSVMDKWLLSKMNTMVKTVDTNLGNYRIPEAARALQEFVDDMSNWYVRRSRERFWAK
                     GMEQDKINAYMTLYTALVTVSKAAAPMIPFMTEEIYQNLVRSVDQNAPESIHLCMFPE
                     VNEDQIDTELEQNMEHVLKLVVMGRACRNTSNIKNRQPIGQMFVKAAFDLPEFYQDIV
                     ADELNVKNVKFTDDVRDFTSYSFKPQLKTVGPKYGKMLGGIKAALDHADGNSAMDEIN
                     ETGALKLDVSGQEITLFKEDLLIDTAQIEGYVSENDNGITVVLDTNLTPELLEEGFVR
                     EIISKVQTMRKEADFEVMDRIKVTYNGSEKAEKIFAEHSDEIGGEVLADKVVKAEPAG
                     YVKEWKINGEAVTMGVEKQ"
BASE COUNT         3471 a         3292 c         2350 g         4365 t
ORIGIN      
        1 aactttgtgg gaaaaccgac tttttccact tttttcgttg ttttcagtgt ggataagttg
       61 aaaactccac gtttttcaat ttcgacattt ttttacaaat aaacacaaat ttgtgcattt
      121 tgtcttttta tgtcgaaaac gggaaatctg tatttctctc atcaaaaata caaattcccc
      181 atttcaaaaa atttgtgtta cagaactctt cttactgcaa taatatcagt ataggtagcg
      241 ctgcatattc cgattccatc tgcttcgttc attgcattca cgatcgttcc gtcccccata
      301 taaagcccga cgtgaccgtc atacaaaatc aagtctccgg gaagagcttc ctcgtaactg
      361 acggcgtatc ctgaattcac catatcatat gtcgtccgtg gaagacttac tccgaaatgt
      421 gcataaaccg actgtacaaa acccgaacaa tccgctccgt ttgtcaggct tgtacctccc
      481 caaacatatg gatttccgat aaactgacaa gcataatcaa taacagcctg ccccggagtt
      541 cccggagctt gcgaaggcgt attcactctc ccctgcgaaa cagcgcctac aggccgtgtt
      601 cccggtctgc cgtcagcacc gcaaacgaca gatgctgcca ttttttcttg cgcttcttct
      661 tctgcgatcc gcttttcttc ctcttctttt gtctccccat aagaataaga agtttctgca
      721 atcacgtagt cgcctgagac ccaaccgctt ttatctccta cttttaccgg ataccatccg
      781 ccggtcgctt ctcccacagt ctcataaact tcgccttttt ttatctgtgt caaaatctta
      841 ctgtccgttc cgcagccttc ccgaacatta agcacatatg ccgtaaccgt cactgtatcc
      901 ttctcatact cctgtgcacg cgcctgcgcc tcctctcctg taaaaagcgc atcttccggc
      961 acatatcctt cagccgaacc cgatctgatc ttcgtccagc catccagata ttccagcacc
     1021 tgagccgccg aatctttata aagtttcccg acccagtcgc cggaatcatc cggtgcgttt
     1081 ctgatatagg taaactcctt cgtatcagaa aacgccatat tcaaatactc accctcttct
     1141 gttggtacaa gatagagatt aatattatct tttacttctg tctcgtaaca tttctccagt
     1201 accgattcaa tccccgatgc ctgtataaat ggctgctcgt ctttcctttc ggccgccgat
     1261 acttcagaca cgctccccgg gatcagaaac attcccgctg ccgcaaacag aatgcctctc
     1321 gcttttgtgt gtggtttctt catataaaac ctcctctata tttcggtcat ttaccgcatt
     1381 acatttatct tttgtaggaa gctttaaatg ttacacattt gttaaatttg ttacattcac
     1441 attatatcaa ctcgctattt gtttgtcaac ttctcttact gatttttcat tgacatctct
     1501 cgacagcaag tttctatatt attctttttt cttcttcttt ccatttgttt cattaattat
     1561 tttgtaacat tttatcgtaa atattcaatt ttaacagcca aaaataattt ttttcaaaat
     1621 aatgattgac aaacatcttt ctttgtatta tattctaatt acagtaatcg ttactattat
     1681 ttattaaata acggttctta gttagttatg aaagggggat tcccattggc agctttaaaa
     1741 tatagtagac aacgagaatc catcaagcat taccttatga caaccaaaga gcatccgacc
     1801 gccgacacag tttatatgca tgtgaagaaa gaatttccga acatcagtct tggcacggtt
     1861 tacagaaatt taaatctgct gaccgatatc ggcgaagcca taaaaatatc tactcccgac
     1921 ggaggagatc gatttgatgg acggattgaa ccgcataatc attttctttg tgaagagtgt
     1981 ggacggttac tggatctgga tctggatatg aagagtatcg aagaagtcaa ctgccttgcc
     2041 ggtaagaatt ttgacggagc gatcacttcc agttccactt tgtttttcgg taaatgcggt
     2101 gactgcatta aaaagtcata acaaataaac aatattattt tcaaaaaagg agatcaaaat
     2161 tatgaaaaaa tttgtatgta cagtatgtgg ttatgttttt gagggagaag cagctccggc
     2221 agaatgtccg gtttgccacg caggcgcaga taaattcaaa gagcagacag gcgaaagaga
     2281 atgggctgca gaacacgtta tcggtgttgc tcagggcgtt agcgaagaca tccttgcaga
     2341 tctgagagca aactttgaag gcgaatgtac agaagttggt atgtaccttg ctatggctag
     2401 agttgcacac agagaaggat atccggaaat cggtctttac tgggaaaaag cagcttggga
     2461 agaggctgag cacgcttcta aatttgctga acttctcggc gaagttgtta cagacagcac
     2521 aaagaaaaac cttgagatgc gtgtagatgc agagaacggc gctacagcag gtaaattcga
     2581 ccttgcaaaa cgtgcaaaag ccgctaacct cgatgcaatc cacgatacag ttcatgagat
     2641 ggcaagagac gaagctcgtc acggaaaagc atttgaagga cttcttaaga gatacttcgg
     2701 ctaatcaaaa tcccgaatat tgaaagatag gttatcagaa agatgtcggc taaactgccg
     2761 gcatcttttt tatctccttt tcttacattt ttttattttt gtctgttttt ttgtttttct
     2821 tttctttctt ttttatttta tatgcattct taccttgtat ctttttttct atctagtata
     2881 atagaaagaa aaggacaaaa aacgaggtgc tatcatgaga aattttattg gtgttgactt
     2941 aggcggaaca aacatccgcg ctgctatcgt ttccgaagac ggaacgatta aatgtatgaa
     3001 aaaatcagaa agccacccgg aacgcggtgc agaagctgtt atggaaacta tgatttccct
     3061 gattgaaagt cttgacggat atgaagaatg tgaaggaatc ggaatgggta ttcccggacc
     3121 gatcgacacg atcaacggca agatcatcgt ttctacaaat cttccaaaac tgatcggttt
     3181 tccgatagcg gaatatatcg aaaatcattt tcataaaccg acttacatgg acaacgacgt
     3241 taaagttgcc gctctcggag aagctgttca gggagccgga aaagattatc ctatcgttta
     3301 ctatgtaaca atttcaaccg gagtcggcgg agcacttgta atcgatcaaa aggtaatcgg
     3361 cggtcagaac ggacacgcag gcgagatcgg aaatatctgt attgacagaa accgtgaaaa
     3421 atacaatatt ttgaatgtag gcgctgttga aaacgaagcg agcggaactg ctgtcacaag
     3481 aaaaggtaaa gctgtattcg gagataagat caatcacgcg ggcgatgtat ttgatcttgc
     3541 ccgctcagga gacgagcagg ctctcaaaat tgtagatgac atggcatatg atcttgcaat
     3601 gatgttctgc gctatcggac atattatcga tcctcatgta ttcgttgtag gcggcggagt
     3661 tatgaaggga aaagatgtat tctttgaaaa aatggaatct tattaccgct ctatgattca
     3721 tgtaggtatg cagccggttg tattcaaaga agctctcctt gatgagccgg gtatcatcgg
     3781 cgctgcgatg cttccgatga cacatttaaa gtaaaactgt ttccttcctt tcaatataag
     3841 cagtttgcta cataaaagaa ctctttttta agttcttaaa aagaaaaact gagcatgacc
     3901 taactctttg agttttcgat catgctcagt ttttcctttc tgctcagtcg cttaatttcc
     3961 cactcccgct tcatcgcttc ctgctttgtc ggaaattctt ccttataaac aagcacaacc
     4021 ggacggcgcg cttttgtgta tttcgcgccc cgccccgcat tgtgatcttt gatccgtttt
     4081 tcaagattgt ttgtccagcc cgtataaagc gaaccgtcac tgcaccttac aatataggta
     4141 taattcatgt cttttattat ttcttttctt tcgtttttct tttcttgtta tatctttggt
     4201 cattgcttct tttattatta cttctttttc tattccataa aattcatcgg atcgaccggc
     4261 tctccgtctt taagcacttg aaaatacaga ttcgttcctt ctacgctgta atatttcgtc
     4321 ggctcattca aactgccgat tgcgctgcct gctgccacat aatctcctgt tttaacttgt
     4381 acatctttta actgtccgta cactgctgaa tatccgtttc ccatatcaag cgtcaccgta
     4441 agtcccgtct gcgccgactc ctcgatcgca gtcacagttc ctgccgcaga tgcattgatc
     4501 gtttctcctg cttccccggc tataatcatc gcaggattat attgatactg ttctaatgtc
     4561 tggaaaaata cagtctcatc catactatat ccattgatca ctgctccgct tgcaggccat
     4621 gtaagaacgc tttcttctga aaaccaaaga tttgacgcgg cgcttcccgt ctgctgaact
     4681 ccttcttccg tccctgttgt atcagacaca tcttccctgc tttcttgttc attttcatcc
     4741 gtttcttcca cttccggcag gaggatttca tctgtagttg tttctttcgt gttatcttct
     4801 gtaaactgtt ccgcctcttt ttcagctttc gccatctgct ctttcatatc ttttttgtag
     4861 tttccgaccg tatatgttcc aacgaccgcc agaactgcca taaagcagac tacaataccg
     4921 atggctgctc ctttgccttt taaaaaggac attttttcat ttttattcat tatcatcacc
     4981 tctggttata tttttgccgg aaaatgataa ctttattctg tcctttcaaa aaagtttttc
     5041 tctgactcta ttgttgatta taattacttt atttattttt tgcaataaag ttcaaatcct
     5101 tctctgtctt tgtcccttca taaaaaaact ccaaaatttc tttatatgtt tttccgtctt
     5161 ttgccatttt atttgcagtc caacaactca gccccatgcc atgtccgttt cccgccgtta
     5221 taatttttat tttcttcccg ctttccgcaa acgaaaaaga actcgacgga agcgacagcg
     5281 catcccgaaa ttgatctcct gtgcaaattg tttctcctat ccgaagcttt cccacatatc
     5341 ccgccgaatc atgttctaaa atttcaaaat ctttaaaaga atatcctgcc gcagcttttt
     5401 ctccgtctgt ctctgccgcc agaaaatgat gacatttttt ctgtatttga tcataggaaa
     5461 acgtaaatac gccgacctgt tcttccgatt ctttatctgc cggacattct ttcactttaa
     5521 tatacggaaa ctccgaactt ccgagcactt cttccgcact gcgcgacatt cccgtatttg
     5581 actgatggaa cggtgtccat gcatatgtat cattataaaa taacacctcg ttttctgtcg
     5641 cttctatggc atttctgtat ttttcatatc gctttttata ttcatcggca ggccattgat
     5701 cctgcatttc cttctgcgac aaataatcga tctccaaaat aacatctgat acatttccgg
     5761 ttctgtccga cttttgattc tccgtctctt ctgcctgagt gcttctttgc agcgcacggc
     5821 tgatctgtgt cctcacaaga accgcctgcg ccttcagtgc ctccgtctct gtatctgccg
     5881 gtacgtcttt tgccaacact cccgtaagat actcattcca ctctacttct gcaatcccgt
     5941 ctgcctcttc ttcctccgaa gctttaactc tgatgtaaaa attttcattc gttgtctctt
     6001 ctggatctac tccgtttaca aacacagaaa caatgtacgg aaacaatatc aaaataacaa
     6061 ggaatgcagc tgccgatttg aatttttgtt ctatcgtata tcttttcatc aaaaaacagc
     6121 cctccatatt tctatcatat gaagcgctgt tttttttata acttttgttt tatcggacaa
     6181 acggtctctt tctcataata aacaggcata tcgcctcgta taacggtact aacgatgata
     6241 atactgcatg aataaccggc atatttcctt ccgcgaagtt ttcataaatc tgtttattca
     6301 ccaagacgtt caaagcgatc tgaactgctc cgaataagat cgaaacaatg atcactacga
     6361 aatacataaa gaaaaacgca agtacgctca tccccgcccc tgtcacattt tgattcaccg
     6421 cacttccggc gagcgtcatt ccgatgccaa atacgagtac cagtacatag atgccgatcg
     6481 ctccgctgta gatgatcgga aaaagcagtt tccacaatcc cggatttttg atcgtcgtat
     6541 ttttcagtcg gatcggacct gccatattcc cgtggtaata tgttctcgca aaaggtacaa
     6601 atgccatcca tgcatctttt ttccccatcc gctttgcgat cgtatacaga ccgaaagaat
     6661 gcagaaggta acttccaagc ataaatacga gcattcctat catgatcgca agccaaagcg
     6721 aagcgaacac tacggcaaac atttctcctg ccctttccgg atgatactga tactcgtatt
     6781 catatgcgta atcatacata tttctcatct tactacctcc ttatcccttt tccgaaaaat
     6841 caatcagatg tttttactct tcaagcggag caacatctac tttttctaat ttccaaatat
     6901 cacttacata ttcttcgatt gttctgtcag aggaaaattt cccgctgcat atcgtattca
     6961 tcattgccat tcttgaccat ctctgctgat ctctgtaagc ttcttccact cgtttctgag
     7021 cctctgcata ggaacggaaa tctttcagaa taaagtaagt atccgctctg tctgtacact
     7081 gcgtattcaa cagagaatta tacagatttt tatacatctc gtgatcgccg tttgcatatg
     7141 tgccgtccat aagctgatcg atcacacgtt ttaattccca atcgttaaag taaatatccg
     7201 tcggattata tcctccgtta ttctcataat taattacttc ttctgaacta aggccgaaga
     7261 taaacgcatt ctccgctccg acttcctgca caatttccac attcgctccg tccatcgttc
     7321 cgagagtcgg cgcaccgttg agcataaatt tcatattacc tgttcccgac gcctcttttg
     7381 acgccgtgga gatctgctcg ctcacatcgg ctgccgcaaa aataatctcc gcatttgata
     7441 cacgataatc ttcaataaat acgactttca gttttccgtt aatgcttctg tcattattta
     7501 caacatcagc tacggaattg atcagtttga tcgtctcttt cgctcttaaa tatcccgccg
     7561 ccgctttcgc tccgaaaata aatgttctcg gataaaacga catctcagga tgttctttga
     7621 tctggttgta cagatacata atatgcagaa tattaagaag ctgacgtttg tactcatgga
     7681 gacgttttac ctgaacgtca aagatcgagc gcggatcaac ttcaattcca ttatgctctt
     7741 taatatattt tgcaagacgt actttgttct cgtatttaat ctgcataaac tcacgtctgg
     7801 ccttttcgtc gtccgcaaac ggtctgagct tttcaatctg cgacagatct gtgatccatc
     7861 catttccgat cttatctgta atccagtctg ccagaagcgg gtttccgtgt gcgaggaatc
     7921 ttctctgtgt gatgccgttt gtcttgttat taaatttttc cggcatcatc tcatagaaat
     7981 cacggagctg ctgtgttttc aagatatctg tatgcagctt cgcaacgccg tttacagaaa
     8041 atcccgcgat aatagccata tttgccatgc gaacctgacc gtctcttaag attgccattt
     8101 tctttacttt atgctcattt cccggataca tctcattcac tttgatcagg aatcttctgt
     8161 caatttcctg cacgatctga taaatacggg ggagcagtct tgaaaacagg tcgatcggcc
     8221 atttttcaag agcttctgcc ataattgtat gattcgtata agcacatgtt ttgctcgtta
     8281 cttcccacgc ctcttcccag cttaatcctt cctgatcgat cagcagtctc atcagttcgg
     8341 gaaccgcaac tgtcggatgt gtatcgttca tctgaatgat tactttctca tgaagtttac
     8401 gaacatcaga atgctttttc ttatatttct caagcagagc ctgaagactt gcggagatga
     8461 agaaatactg ctgtttcaaa cggagttctt ttcccgaata atgattgtcg ttcggataaa
     8521 gaacgtcaac gatcaatttc gcaagatttt cctgctctac cgccttatgg taatttcctc
     8581 tgtcaaattc atcaagctta aaatctgtga tcgcttttgc atcccacaca cgcagtgtgt
     8641 ttacgacatg atttccatat cctacgatag gcatatcata cggaatcgcc aaaacagact
     8701 cataattttc ctgcacaaat tttgcttttc ccgtctcagg gtcattttca aaccggatat
     8761 tccctccgaa gcggacttct tttgcatatt cttctcttct gatctcgaac ggattacctt
     8821 ctttgagcca gttatccggc acttccacct gatacccgtc tttaatcttc tgtttaaaca
     8881 tgccgtaacg ataacgaatg ccgcatccat aagccggata attcaatgtt gcaagtgaat
     8941 caaggaaaca ggctgccaac cgtccgagac ctccgtttcc aagcgctgca tccggctctt
     9001 gatcctcgat tacattcaga tcgatctcca tttcttccag cgcctctttt acctctttat
     9061 acgctgtcag attgatcagg ttatttccca atgcacgccc catcaaaaac tccatagaca
     9121 tgtaatacac agtctttgca tctgcctcgt catacgcttt ctgtgttgcg atccaatcat
     9181 cgatgattac gtctttcact gcataggata ctgcctgaaa aatctgctga ggagttgcct
     9241 cgtcaatcgt ttttctgtac agcgtcttaa tattttctct tacggtttcc ttaaatgttt
     9301 ctttctcaaa tcctttatta tgcattgctt tattcccttc cttttgatat gccgcggcgc
     9361 atcgcatcct ttatcttaca agaacgaatg cgcccggatg tgccgcatat cttaaaatta
     9421 ttgtttctca acgcccattg taactgcttc tccgttgatc ttccactctt tcacataacc
     9481 tgccggctct gcctttacaa ctttatctgc aagcacttcc ccgccgatct cgtcactgtg
     9541 ttctgcaaag atcttttccg ctttctcact tccgttatac gttactttga tgcggtccat
     9601 aacttcaaag tcagcttctt ttctcattgt ctgtacttta ctgataattt ctctgacaaa
     9661 tccttcttca agaagttccg gtgtcaggtt cgtatcaaga acaaccgtga ttccattgtc
     9721 gttttcagaa acatatcctt cgatctgcgc tgtatcgatc agaaggtcct ctttgaaaag
     9781 tgtgatctcc tggccgctta catcaagctt aagcgctcct gtttcattga tctcatccat
     9841 agcagaattt ccgtctgcat gatcaagcgc cgctttaatt ccccctaaca ttttaccata
     9901 tttcggacct actgtcttta attgtggttt aaaactataa gaagtaaagt ctcttacatc
     9961 atctgtaaac tttacatttt ttacattcag ctcatcagct acgatatcct gataaaattc
    10021 aggcagatca aatgctgctt ttacgaacat ctgaccgatc ggctgacggt tcttgatgtt
    10081 cgatgtattt ctgcaggcgc gtcccataac gacaagcttc agcacatgct ccatattctg
    10141 ctcaagctct gtgtcgatct gatcctcgtt cacttccggg aacatgcaca gatggatact
    10201 ctccggcgca ttctggtcaa cgctgcgtac aagattctga taaatctctt ctgtcataaa
    10261 cggaatcatc ggagctgccg cttttgatac cgtaacaagc gctgtatata gtgtcatgta
    10321 tgcattgatc ttatcctgct ccattccttt cgcccagaaa cgttcacggc ttcttctcac
    10381 ataccagtta ctcatatcat ctacaaattc ctgaagagcg cgcgccgctt ccggaatacg
    10441 ataatttccg aggtttgtat ctaccgtttt taccatcgtg ttcatctttg acaaaagcca
    10501 cttatccata acggacagtt tattatattc taattcatat tttgtggcgt caaactgatc
    10561 gatatttgca taaagaacaa agaaagcata tgtgttccac agtgttccca tgaactttct
    10621 ctgaccttct actacagctt ttccgtggaa acggttcgga agccacggag cgctgttcac
    10681 atagaaatac cagcggatcg catctgcacc gtatttttca agagcatcaa acggatcgac
    10741 tgcatttcct tttgatttgc tcattttctg tccgttctca tcctgtacat gaccaagtac
    10801 gattacattc ttatacggcg ctttgttaaa cagaagcgtt gagatcgcaa gcagggaata
    10861 gaaccatcct ctcgtctggt ctaccgcctc cgaaataaag tccgccggga actgtctttc
    10921 aaacagctct ttattttcaa acggataatg atgctgtgca aacggcattg atccgctgtc
    10981 aaaccagcag tcgattactt ccggaacacg gtgcatctct tttccacact tcggacattt
    11041 gatcgtcacc gcatcaatgt acggacgatg aagttcaatg tcatccgggc aattatcgga
    11101 catttctttt aattcctcaa tgcttccgac agaatgcata tgtccgcact cgcattccca
    11161 aatattaagc ggcgttcccc agtaacggtt acggctgata ccccagtcct gcacgttttc
    11221 aagccagtcg ccgaaacgtc cttttccgat actttcagga atccagttga tcgtattatt
    11281 atttgcgatg aggtcctctt tcacggcagt catcttgatg aaccatgatt cacgtgcata
    11341 atagatcagc ggtgtgtcgc atctccagca atgaggataa ctatgctcaa atttcggtgc
    11401 atcatagagc agacctctgg catcaagatc tttgagcact tccggatcgg ctttctttac
    11461 aaacaatcct gcaaacggcg tctcctctgc catatttcct ttttcatcta caagctgaac
    11521 aaacggaagg tcatattttc ttcctacgtt cgcatcgtct tcaccgaatg ccggagcaat
    11581 atgaacaacg cctgtaccgt ctgtcaacgt aacatattcg tcacatacaa cgtaaaacgc
    11641 tttcttgttc tgcttctgcg cacaatctgc cgcgcactga taaagcggct cgtattcttt
    11701 tccttccaaa tcagttccct tatatgtctc aagcacctca tacgctgctt ttccttcttc
    11761 tgcaagtctt ccaagcaccg tatcaagaag cgcgcacgcc atataataaa tatttccgtc
    11821 cgcagccttt actttcgcat aatcttcttt cggatttaca caaagcgcaa ggttcgacgg
    11881 cagcgtccag ggagttgtcg tccatgcaag gatatacgca tcttcttctt ttactttgaa
    11941 tctgacgatc gcagaacgtt cttttacatc tttgtatccc tgtgcaactt cctgtgcaga
    12001 aagcggcgtt ccgcagcgcg ggcaataagg aacgatctta aatcctttgt aaagaagccc
    12061 tttatcccag atctgcttta acgcccacca ttccgactct ataaaggaat tatgatatgt
    12121 gacatacgga tcgtccatat ctgcccagaa acctactgtt cctgaaaaat cttcccacat
    12181 tcctttatat ttccaaacac tttccttaca cttatcaatg aaaggttcta gtccatactc
    12241 ttcaatctgg tctttcccgt caagtccgag cagcttttcc acttccagtt ctaccggaag
    12301 tccgtgtgta tcccaacctg cttttctggg aaccatactg cctttcattg ttcgataacg
    12361 cggaatcata tctttaataa cacgagtcag cacatgacca atgtgcggct ttccgtttgc
    12421 tgtcggaggg ccgtcataaa aaatataatc cggacatcct tcccgctctt ccatactttt
    12481 ctcaaaaatg tgattgtcgt cccaaaactt ttcgatttgt ttttctcgat cgacaaaatt
    12541 catgtctgta gaaacttttt tgtacatgat cttcttcctt tcctccttaa taacaaaaag
    12601 cttccgtcct gtaataggac gaaagcttat tctccgtttt taaccacctg ttacaacata
    12661 cttggatctc acgccttgac tgtacgcgac agaaaacatc tatttacagt atagtacatc
    12721 tctttctata tctcttcttt tctgtagctt atgcatacga ttcggatccg catatggtgt
    12781 cctgtaacgt cggacttacg tcggctctta cttctctgta tttatttcag atgccgcggc
    12841 tcggaagtga tattcagaat ctgtctaccg gtatcggact tacaccatcc ccgactcgct
    12901 gtaacattcg atcagatctt actgtctccg tcatcgcctt tttcatcata tttttaatat
    12961 gtcgtataaa atattataaa aaaaatatta atattagtca aggtggtttt tccgtaaact
    13021 tattataatc gtaacagttc agccacaatt atttagaatc agtatacgaa aaaaagaact
    13081 gttctactag actatccatg caaaaactgt tgttgtattg tatcgggtaa ttttgaggga
    13141 attcctcaga ccggtgaagc tgccggtatt tttcacagaa agaatttctg tgagtggaat
    13201 tatcatagca tatcggtaaa tgagaaacaa tgatatattt atattttaat cgacttctga
    13261 cagaggagaa atcaactctc tcctctgttc tatgaagtta tctttgatta atttatatgg
    13321 agatgaaact gttcaatcag atgagcaata ttttcttctg tattttcaag atcatctgca
    13381 atttcggaaa ttgtttttcc tttttcaagt tttttccgga tgagagcact tttttcttct
    13441 attcgtcctt cttctcttcc cttcttctgc ccttcttc
//
LOCUS       DBMDXF010000033        13086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1381_length_13086_cov_1.883943, whole genome shotgun sequence.
ACCESSION   DBMDXF010000033 DBMDXF010000000
VERSION     DBMDXF010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_13086_cov_1.883943"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(235..1122)
                     /locus_tag="ACZPD4_02485"
     CDS             complement(235..1122)
                     /locus_tag="ACZPD4_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HIR1377979.1"
                     /translation="MEHCKIIAVGDNVCDKYLSRGKMYPGGQCVNTCVYVKMNNMESA
                     YLGKYGDDEVAACVQDTLKEIGIDDSHCRRYEGENGFALVTLKETDRVFLGSNKGGIA
                     KEHDFGFTKEDLEYIKGFHLIYTNLNSYIEKELPVLYQTGVPVAYDFSTRWTDEYLKE
                     ICPYVTVAIMSCAHLTDEEREKEMRKAQSYGVKVVLGTIGEDGSYVLYNDKILYAPAV
                     HADDVIDTMGAGDSYFSAFLCSLLETSKEGKIVEGTDEEMAKRLQTAMGKGAVFAAKV
                     CALEGAFGYGVPILGKTTV"
     gene            complement(1255..2043)
                     /locus_tag="ACZPD4_02490"
     CDS             complement(1255..2043)
                     /locus_tag="ACZPD4_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1377980.1"
                     /translation="MKRTLAMADNCIDVYYKLDRYYLTGNSIDFALNYKDLGGDVTEI
                     TILGNDVFADALEERLKERKIPLRILKRTDRPTAAAKMDLVDGDKKHLHFEGNAMEEI
                     ELQDDDLEFVKQFDIIYAERWAKVGRYIKEIRQEGQIWVYDFSKRLEQESNDEIIPYL
                     DYAFFSYDRDDEYVRNFMVKTSEKGAKTVVAMLGEYGSLAYENGKFYKLDAEKVEVVN
                     TVGAGDSYIAAFTYGVSLGEDIMQCMRRGRQRATEIIQQFNPYK"
     gene            complement(2051..2488)
                     /locus_tag="ACZPD4_02495"
     CDS             complement(2051..2488)
                     /locus_tag="ACZPD4_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR1377981.1"
                     /translation="MDFILKEVPICADKEFEMIKITDDVRKVVEESGVEKGIVYVITE
                     HTTTGITVNESLPCVEKDLMECLDRIAPEDYPYHHNHYLPTYGTIGGNAPGHLKSLLT
                     GNHCVFPVLDGTLKLGHAVDIYFCEYDGIKNRRYMVYVVGERG"
     gene            complement(2494..3342)
                     /locus_tag="ACZPD4_02500"
     CDS             complement(2494..3342)
                     /locus_tag="ACZPD4_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1377982.1"
                     /translation="MVIDTHIRPALFSPICENKERFEKRCDQMNYHLMKPSSIELLKK
                     QYALAGIEKVFLLPSDCSFETGEPDISNDEIEKIVQCDPTFFVGFASADPRKESAAEE
                     LEKAFKFQNLAGLYINTARLHMYPCDERLFVLYDICKKYKRPIIFQAGLSMENNSLAK
                     YCRPIEFEEVLSKYPEVNICLSHVGWPWVQETAALLLKYENCYTNTALMNFDGPYQIY
                     KKVFTEDMGALWVEHNIADKIMFGSGSPRIRPVRSKRGLDSLGFSEETLEKIYAENAE
                     RFLGRE"
     gene            complement(3489..5024)
                     /locus_tag="ACZPD4_02505"
     CDS             complement(3489..5024)
                     /locus_tag="ACZPD4_02505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1377983.1"
                     /translation="MKEYYLGIDIGTYESRGMLIDENMIPVADCSVTHGMDHPQEGWF
                     EHDADKVWWGDFCKLCRLLLEKSNIRSDEIRCVGTSALGTDCLPVDKDCNPLRPAILY
                     GIDSRAEEEIKWLTQYYGDDVKKMFGHPICTGDTAAKILWLKNHEPEVYEKTYKFLTG
                     SSYLTAKLTGKYVIDQFLAKGSFRPLYNRDGTINKENCSLYCRPDQIAECAYSFEIAG
                     TVTEEAAKESGLKKGTPVIVGTGDSTAEAISVGLVESGTVFFQYGSSMFYYYCVDRYV
                     DDYVSANGNGSLKGGKEFTIPGTFCIGDGTNAAGTLTRWVRDTFYEEELKKERAGGEN
                     AYAVMAREAAEVEAGSEGLIILPYIYGERSPIQDAKASGMLFGLKGTHTRKQINRAAL
                     EAVGYSTLQHMILFDEMNLPPKSVITAGGGTKNAAWMQIICDMIGRQICIPKRYQCSA
                     YGDAAMAALGDRRFRDFTELRDSLPKGKILVPNEKNHIYYKEHYKMFRDLYLNNKDMM
                     HRI"
     gene            complement(5093..5902)
                     /locus_tag="ACZPD4_02510"
     CDS             complement(5093..5902)
                     /locus_tag="ACZPD4_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1377984.1"
                     /translation="MWTQDMFGVEKPIIALLHLDALPGDPGYCGDMKTVTEHARKDLL
                     ALQDGGVDGILFANEFSLPYQPVADIAVVSAMAYIIGKLKDEISVPFGVNVVKNPIAT
                     IDLGAATGAKFGRSCFSGAYMGEYGVYVSNSGEAIRHRKALGIEDMKLLFKVNPEADA
                     YLVQRDVQVVAKSIMFGDFADGLCVSGAAAGAEPDDVILSRVHEVAKPRKVPVFCNTG
                     CNHGNVREKLGNCDGVCMGTAFKKDGVFNGRVDKERVREFMEIVADIRKGL"
     assembly_gap    6129..6138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6139..6768)
                     /locus_tag="ACZPD4_02515"
     CDS             complement(<6139..6768)
                     /locus_tag="ACZPD4_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1377985.1"
                     /translation="MKFAPMNYHYLRYPIKKFLDKVERSPFNSIDLYCSAPQLNLFDY
                     PLSRLIELKKEIEAHHLSVMAMTPENCVYPVNFCTQDDLTRESSIRYYQRAIDTAQFL
                     DCPCVQISTGFGYFDQPREEGWKYCRESLWTLAQYCEKKDVRLLLEELKVTTTNVLIT
                     SKDIAKMLEEIDSPAIVGMVDMDQMTYAKETIDDYFDNLGDKLQHIHFND"
     gene            complement(6902..8215)
                     /locus_tag="ACZPD4_02520"
     CDS             complement(6902..8215)
                     /locus_tag="ACZPD4_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HIR1377986.1"
                     /translation="MELIIVLAIIFIGVRVGGTFLAMAGGIGMFIMTFILHVTPSDPP
                     ITVILIMIAVICAAATMQACGGLDYLVRIAEKILRKNPKMITVLAPVVAYIFTFMCGT
                     GHIVYSLLPVINEIAIETGVRPERPISASIVSSQQAITACPISAATVGILAFMAEATN
                     YTKVNIFTLLIVCVPATLIGTVIAAIAVIKKGKELADDPEFQARVEAGLIEDFSKKVK
                     EEKKVSKEAKISVTIFLIAMVGIVLLGAVSGLVPTLADGSKLPLTTVIEIFMLVAAAA
                     MILLTKLDSNKVLDQPVFRTGMFAVVLAFGLCWLVNTFIGDQSSFITDNMSALTNKYP
                     WIFIVAVFIVGAITTSQSSTTMIMIPIGIALGLPANVIVAGWIACSSNYFIPASGQCV
                     AALAFDSAGTTKIGKFVLNHSYMIPGLVCTVVSVVVALLIGAVVF"
     assembly_gap    8218..8227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8624..9634)
                     /locus_tag="ACZPD4_02525"
     CDS             complement(8624..9634)
                     /locus_tag="ACZPD4_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1377987.1"
                     /translation="MSYVSTDIKKIIAEIIENKKEKGGVKNIYFVGCGGSLGALYPAK
                     TFMEREAQVLRSAWINSNEFVHSTPKDFGENSIICLACHKGNTPETIQAAKLGKEKGA
                     AVIILTWLEESEIIEFGDYIIRYTFDASLDHLAGDIDYAGEKTQCALLVAVELLAQTE
                     GYENYEKFYEGLGMISNIIKNARKHVAERAEAFAEEYKNDSVIYTMGSGASYGAAYME
                     SICIFMEMQWLDSSSIHTGEYFHGPFEITDANRPFMIQISEGTTRELDERALTFLKKY
                     AKRIEVLDAKELGLSTIDASVVDYFNHALFNNVYPIYNHALATKREHPLATRRYMWKV
                     EY"
     gene            9798..10508
                     /locus_tag="ACZPD4_02530"
     CDS             9798..10508
                     /locus_tag="ACZPD4_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1377988.1"
                     /translation="MELNNTIATPLYKQLEEKLQHEIETGERPAGSRLPTENELSETY
                     HVSRVTVRKALAELSELGYLDRKSGKGTFVAEKKLHRSITEVISFTDMCRMMNATAGA
                     KTIKIALEEPSEREIKEMSLKPGEKILVLERIRYMDDEPVIIELNKFPESFSFLFGED
                     LNDTSLFEVLKKHNIILDHSRKTLDIIFASPQEAKALNINKGYPLLRIKSTIHDVENT
                     ITNLCQQLCIGDKFTFIV"
     gene            complement(10667..11257)
                     /locus_tag="ACZPD4_02535"
     CDS             complement(10667..11257)
                     /locus_tag="ACZPD4_02535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR1377989.1"
                     /translation="MTEKEKMQSQKLYDANYDSELERERIECKALCQEYNHLPIKETE
                     KRSRLIKKILGKTGEKIHIEPDFWCDYGYCISAGENFYANHGLVVLDAGGVAFGDNVF
                     VAPNCGFHTSGHPIDFERRNRGLEYAYPIKVGNNVWFGAGVQVMPGVTIGSNVVIGAG
                     SVVVKDIPSDSVAVGNPCKVIRPITEEDKKKCWDRT"
     gene            complement(11351..11476)
                     /locus_tag="ACZPD4_02540"
     CDS             complement(11351..11476)
                     /locus_tag="ACZPD4_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024945.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1377990.1"
                     /translation="MHIHRNSFLYRINKIEEITGVDLREYHTFIHLAINIYIEEI"
     gene            complement(11498..12958)
                     /gene="abc-f"
                     /locus_tag="ACZPD4_02545"
     CDS             complement(11498..12958)
                     /gene="abc-f"
                     /locus_tag="ACZPD4_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIR1377991.1"
                     /translation="MSMIRVENITFSYPSSFDPVFENVSFQIDTEWKLGLVGRNGRGK
                     TTLLKLLLGEYEYRGKITGAVTFDYFPFPVSDKTQMTEDILRQVCPNARQWELIRELS
                     YLGIESEVLWRPFETMSNGEQTKALLAALFLKEEHFLLIDEPTNHLDVEGRRQMADYL
                     KRKRGFILVSHDRYLLDECVDHILSLNRSNIEIQSGNFSSWMENFKKRQNFEINRNES
                     LKKDIRRMKESARKAKVWSDKVESSKRGAADKGYVGHKAAKMMKRSKNAEARKERAAE
                     EKSKLLNNAEYAEMLKITPLMYHSEKLVSFSDVAVIYEGNQVCDAVTFQICRGERILI
                     QGKNGSGKSSLLKLLLKYPIEHTGTVTSGSGLVISYVPQDTSHLKGTLTEFAKVHKID
                     ESLFKAILRKMDFERVQFEKDMKDFSAGQKKKVLIAKSLCEPAHLYVWDEPLNYIDVY
                     SRIQIEQLIRQFSPAMLLVEHDVTFQKEIDAKVITL"
BASE COUNT         3481 a         3194 c         2073 g         4318 t
ORIGIN      
        1 tcgatatcat tatccggctt ccagttttta atgcttacaa gccatttttc attcgtatat
       61 acacatttga ttccttcttc tttgttttca tatacattcg gtctcatttt ttcctccgtt
      121 attatactat atttttcatt atttttaact ggttgatctc tcttttacag ttatcttttt
      181 atagctatcc tttttatctt ttcttttatc cttttgtatc ttacctttgg atctttacac
      241 agttgtcttt cccaagatcg gaactccgta tccgaaagct ccttctaaag cacatacttt
      301 cgccgcaaat accgcgcctt ttcccattgc cgtctgaagt ctttttgcca tttcttcatc
      361 tgttccctca acgatctttc cttcctttga cgtttcaaga agcgaacata aaaatgctga
      421 gaaataagaa tctcctgctc ccattgtatc gatcacatcg tcggcgtgta ctgcaggcgc
      481 atataagatc ttatcattat aaagaacgta agatccgtct tctccaatcg ttccgagaac
      541 aactttcact ccgtaagact gtgcttttct catttctttt tcccgctctt catctgtcaa
      601 atgcgcgcac gacataatcg caactgttac atacggacat atttctttca aatactcgtc
      661 tgtccaacga gtagaaaaat catatgcaac cggaacgccc gtctgatata aaacaggaag
      721 ttctttttca atataagaat tcagatttgt gtaaataaga tgaaaacctt tgatatactc
      781 taaatcttct tttgtaaatc cgaaatcatg ctcttttgcg ataccgcctt tatttgaacc
      841 taaaaatact ctgtcagttt ctttcaaagt tacaagagcg aatccatttt caccttcata
      901 acgacggcag tgactgtcat caattccgat ttctttcaac gtatcctgaa cacaggctgc
      961 cacttcatca tctccgtatt ttccaagata cgcagactcc atattgttca tctttacata
     1021 aacacatgta ttaacgcact gtccgcccgg atacatcttt cctcttgaca aatatttatc
     1081 acatacgtta tctccaactg caataatctt acaatgctcc atctttccga ttcctttctt
     1141 ttctgacatt ttctgcaaca gatcgcctaa tcgaaactgt caccgctgaa ttgttactct
     1201 tttttcatgc cgtattttgt ttctttgttt tccggcattt cacgatattt ttcgctactt
     1261 ataaggattg aactgctgta taatctctgt cgcacgttgt cttccgcgcc gcatgcactg
     1321 catgatatct tctcccaagc tgactccgta tgtaaaagcc gcgatataag aatcacctgc
     1381 tcctaccgta tttactactt caactttttc tgcatccaac ttataaaact tcccgttctc
     1441 ataagcaagg cttccgtatt ctccgagcat ggcaacgact gttttcgccc ctttttcgct
     1501 cgtcttcacc atgaaatttc ttacatattc gtcatcccga tcataagaaa agaatgcata
     1561 atcaagatac ggaatgatct cgtcgttact ttcctgttcc aaacgtttag aaaaatcata
     1621 tacccatatc tgcccttcct gcctgatctc ttttatatat ctgccgactt tcgcccaacg
     1681 ctccgcataa ataatatcaa actgttttac aaattccaga tcatcgtcct gaagttcgat
     1741 ctcttccatc gcatttcctt caaaatgcag atgcttttta tccccgtcaa caagatccat
     1801 ttttgcggcg gctgtagggc ggtcggtacg ctttagtatc ctcaaaggaa tttttctttc
     1861 ttttaacctc tcttctaaag cgtcggcaaa cacatcattt ccgagaatcg tgatttccgt
     1921 gacatcaccg ccaagatctt tataattcaa tgcaaaatca atactgtttc ccgtcagata
     1981 gtaccgatct agtttataat aaacgtcaat gcagttatct gccatcgcaa gtgtccgttt
     2041 cataatgatc ttaacccctt tccccgacta cataaaccat ataacgtctg ttttttatcc
     2101 catcgtattc acaaaaataa atatcgactg cgtgtccgag ttttaatgtg ccgtctaaaa
     2161 caggaaatac gcagtgattt cctgtcagaa gcgatttcaa atgtccgggc gcgtttcctc
     2221 cgatcgttcc gtaagtcgga aggtaatgat tatgatgata cggataatct tcaggcgcga
     2281 tccggtcaag acattccata agatcttttt ctacacacgg aagactttcg tttacagtga
     2341 ttcctgtcgt tgtatgctct gtaattacat agacaattcc tttttcgact ccgctttctt
     2401 ccacaacctt gcgtacgtca tctgtaatct tgatcatctc aaattcttta tccgcacata
     2461 tcggaacttc ttttagtata aaatccatct ctctcactcc cttccaagaa accgctctgc
     2521 attttctgca tatatttttt caagtgtttc ttccgagaaa ccaagactgt ccaatcctct
     2581 tttacttctg accgggcgaa ttctcggcga acccgaacca aacataatct tatccgctat
     2641 attgtgttct acccaaagcg ctcccatatc ttcggtaaat actttcttgt aaatctgata
     2701 cggtccgtca aaattcatca gcgccgtatt tgtatagcaa ttctcatatt ttaaaagcaa
     2761 cgccgccgtt tcctgaaccc acggccagcc gacatgagaa agacagatgt tcacttcggg
     2821 atatttactt aaaacctcct caaattcaat cggacggcaa tattttgcca gagagttatt
     2881 ttccatactc aatcctgcct gaaaaatgat cggcctttta tatttcttac atatatcata
     2941 cagcacaaat aatctttcat cacagggata catatgtaat cttgccgtat tgatataaag
     3001 tcccgccaga ttttggaact taaacgcctt ttccagttct tctgccgccg attcctttct
     3061 cggatctgca gacgcaaacc cgacaaaaaa cgtcggatca cattgtacga tcttctcgat
     3121 ctcgtcgttt gatatatccg gttctcccgt ttcaaacgaa caatctgacg gaagcagaaa
     3181 tactttttca attcctgcca atgcatattg ctttttcaaa agttcgatac ttgacggctt
     3241 catcaaatga tagttcatct gatcgcatcg cttttcaaag cgctctttat tttcacagat
     3301 cggtgagaaa agcgccggac gaatatgtgt gtctattacc atttttgtca cttcctctta
     3361 tttttcgctt ttatttttcg cttttatttg ccgcatttct tttatatatc tcgtttctta
     3421 tttcttttgc tttattttgt tgctttttat tttttgcttc aatttgtttt cttctatatt
     3481 gaaattgttt aaattctatg catcatatct ttattattca aatacaagtc tctgaacatc
     3541 ttgtaatgtt ctttgtaata tatatggttc ttttcattcg gaacaagtat tttccctttc
     3601 ggaagagaat ctctaagctc cgtaaaatca cgaaacctcc gatctccaag cgccgccatt
     3661 gcagcatctc cgtatgccga acactgatat cttttaggaa tacatatctg ccttccgatc
     3721 atatcgcata taatctgcat ccatgccgca ttttttgtgc cgccccctgc cgtaataacg
     3781 gatttcggcg gaaggttcat ttcatcaaac aagatcatat gctgaagagt cgaatatccg
     3841 accgcttcaa gcgcggctct gttaatctgc tttcttgtat gtgttccttt taaaccgaat
     3901 agcattcccg atgctttcgc atcttgtatc gggctgcgct ctccatagat ataaggaagt
     3961 atgatcagcc cttccgatcc ggcttctacc tctgccgcct ctcttgccat cactgcgtat
     4021 gcattttcac cgcccgctcg ttccttcttc aattcttcct cataaaacgt atccctcacc
     4081 catctcgtca gcgttcccgc cgcattcgtt ccgtctccga tacaaaatgt tcccggtatc
     4141 gtaaattcct ttccaccttt taaagaaccg tttccgtttg ccgacacata atcatcaaca
     4201 taacgatcaa cacagtaata ataaaacata gacgaaccat actggaaaaa tacagttccc
     4261 gattctacca atcctacgct gatcgcttct gcagtggaat ctcctgtacc gacgatcact
     4321 ggcgtacctt ttttcaaacc actctctttt gccgcctctt ccgtcactgt ccctgcaatt
     4381 tcaaaagaat acgcgcactc cgcgatctga tccggccgac aatataagct acaattttct
     4441 ttatttatcg tgccgtcccg attataaagc ggacgaaacg atccttttgc caaaaactga
     4501 tcgatcacat actttccggt taattttgca gtgagatatg aacttcccgt caaaaactta
     4561 tatgtctttt catacacttc cggctcatgg tttttgagcc acaaaatttt cgccgccgta
     4621 tcgcctgtac agatcggatg accgaacatt tttttgacgt catctccata atattgagtc
     4681 agccacttaa tctcttcttc cgcccgcgaa tcgatcccgt ataatattgc aggacgcagc
     4741 ggattacagt ctttatctac aggcaggcag tccgtaccaa gagcgctcgt cccgacacat
     4801 ctgatctcgt cagaacgaat gttactcttt tcaagaagaa gcctgcacaa tttacagaaa
     4861 tctccccacc agactttgtc cgcatcatgt tcaaaccaac cttcctgcgg atgatccatt
     4921 ccatgagtta ccgaacaatc tgcaacaggg atcatatttt catcgattaa cattcctctc
     4981 gactcatatg taccgatatc tattcccaga taatattctt tcactgtttt ctcctcaaag
     5041 actcttcttt cactgttttt cttatcaaag attcttcttt attgattttt ccttacaatc
     5101 cttttcttat gtctgccacg atctccataa attctcttac acgctcttta tctaccctgc
     5161 cgttaaatac gccgtctttt ttaaatgcag ttcccatgca gacgccgtcg cagtttccca
     5221 gtttctctct gacatttccg tgattgcatc ctgtattaca aaataccgga acttttctcg
     5281 gcttcgccac ttcatgcact ctggatagta tcacatcgtc cggttctgct cctgctgccg
     5341 ctcccgacac acataatcca tctgcaaaat cgccgaacat aatggatttt gcaacaacct
     5401 gaacgtctct ttgtacaaga taagcatccg cttccgggtt tactttaaaa agaagtttca
     5461 tatcttcgat tccgagcgct tttctgtgac ggatcgcttc tccgctgttc gagacatata
     5521 ctccatattc tcccatatat gctcctgaaa aacagcttct tccgaatttt gctcctgttg
     5581 cagctccgag atcgatcgtt gcaatcgggt tttttacgac attaacgcca aacggtacgc
     5641 tgatctcgtc ttttaatttt ccgatgatat acgccatagc cgaaacaacc gcgatatccg
     5701 ctaccggctg gtacggcaaa ctgaattcgt ttgcaaaaag gattccgtca acgcctccgt
     5761 cctgcagcgc cagcaagtct ttccttgcgt gttctgtaac tgttttcata tctccgcaat
     5821 atcccggatc tcccggaagc gcgtcaagat gcagcagcgc tataatcggt ttctctactc
     5881 caaacatgtc ttgtgtccac ataacacttt cctccgtata atatttgttt ctatgttgta
     5941 ttctccttta tataacaaga tttgtaattt ataattaatc aaactcgttc gtatttgctt
     6001 tcatccatgc gacaatatca tcgatcgcct tatcaggatc gcgataatat cttctgtcgc
     6061 atatttcaaa cgacaccgtt ccgtcataac ctctgttttt aatcgaatca taatattctt
     6121 tcatcgggnn nnnnnnnngt cgttaaagtg aatatgctga agtttatctc cgagattatc
     6181 aaaataatca tcgatcgttt ccttcgcata tgtcatctga tccatatcga ccattccgac
     6241 gattgccgga gaatcgatct cttccagcat ctttgcgata tctttgcttg tgataagcac
     6301 gtttgtcgtc gttactttta actcttccaa aagtaacctg acgtctttct tttcgcaata
     6361 ctgcgccagt gtccacaacg attctctgca gtatttccaa ccctcttctc ttggctgatc
     6421 gaagtatccg aatccggtac tgatctgcac acacggacag tcaagaaact gtgccgtgtc
     6481 gattgctcgc tgataatacc gaatgcttga ctctcttgta agatcatcct gcgtacagaa
     6541 attaaccgga tacacacagt tttcaggcgt catcgccatc acacttaaat gatgtgcttc
     6601 aatctccttt ttcaattcga tcaatcttga aagcggataa tcgaacaaat taagctgcgg
     6661 cgccgaacag tacagatcga tactgttaaa cggtgagcgt tccactttat caagaaattt
     6721 tttgatcgga tatcttaaat aatgataatt cataggcgcg aatttcattg ccatattcct
     6781 ccgtttcaat acttaaaatt tgaaaagact gccgcccgtt ccatacggat acaaggctgc
     6841 agtctctttt tgcttttcag ctttctttct atattgccgc agcacattgt cttctgactt
     6901 cttagaatac aaccgctcct ataagaagtg cgactactac tgatacgacc gtacatacaa
     6961 gccccggtat catatagctg tggttcaata caaatttacc gatcttcgtt gttcccgccg
     7021 agtcgaatgc aagagctgca acacactgtc ccgaagccgg aataaagtag ttagatgaac
     7081 atgcgatcca acctgccacg attacgtttg ccggaagtcc taaagcgatt ccgatcggaa
     7141 tcatgatcat tgttgtcgaa gactgactcg ttgtgatcgc acctacgata aataccgcta
     7201 caatgaaaat ccacggatac ttatttgtca gcgccgacat attgtctgta ataaatgacg
     7261 actgatctcc gataaatgta tttacaagcc agcacagacc aaacgccaga acaaccgcaa
     7321 acatacctgt tctaaatacc ggctgatcaa gaactttgtt gctgtccagt tttgtaagaa
     7381 ggatcatagc cgccgcagct acgagcataa agatttcgat tactgtcgta agcggaagtt
     7441 tgcttccgtc tgccagcgtc ggaactaaac cgctgactgc gccgagaaga acgattccca
     7501 ccatggcaat gaggaagatc gtaacggaaa tttttgcttc tttgctgact tttttctctt
     7561 cttttacttt cttggagaaa tcctcgatca atcctgcctc aacacgagcc tgaaattcag
     7621 gatcatctgc aagctctttg cctttcttaa ttactgcgat tgccgcaatg actgttccga
     7681 tcaatgtagc cggaacacaa acaataagta atgtgaagat atttactttc gtataatttg
     7741 ttgcctctgc catgaaagcg aggattccga ctgttgccgc tgaaatcgga cacgctgtga
     7801 tcgcctgctg tgaagaaacg atcgatgcgg aaatcggtct ttccggacga acgcctgtct
     7861 caatggcaat ttcgttgata accggaagaa gactgtaaac gatatgacct gtaccacaca
     7921 taaatgtaaa gatatatgct acaaccggag ccagtaccgt aatcattttc gggttctttc
     7981 tcaggatttt ctccgctatg cggacgaggt aatccaaacc tccgcaagcc tgcattgttg
     8041 ccgccgcaca aataactgct atcatgatca aaattactgt gatcggcgga tcagaaggag
     8101 taacatggag aataaatgtc ataataaaca ttccgattcc tcctgccatc gcaaggaacg
     8161 tacctcccac acgaactccg ataaaaatga tcgccaaaac gataatcaat tctatccnnn
     8221 nnnnnnnatc actctttagt ttaccctcat tttgaatttt ccttttaaag aactatgcgc
     8281 atattcttta actctacaaa ccaatccagt gcattgtatt cttttgtatc gttctgtatt
     8341 gttttgtatc tgatacaata gtatatttgt tttgttgttt tgtcaattta tttttgcaac
     8401 tttgtgtatt ttatatgaat attaattaca tttttgtgca tgatgctgta tttctattat
     8461 tttgtattat ttatatgtat tacctatatt ttccaatttt tttcatacca taagcattac
     8521 tattcgccca atttaaaaga agcccgcaca atgtgcagac tccttttata aaataagtta
     8581 tatcgcttaa ttggtcataa ataatttgtt tttatatccg tttttaatac tctactttcc
     8641 acatatagcg gcgtgttgca agcgggtgct cacgttttgt cgcaagagca tggttgtaga
     8701 tcgggtatac attgttaaac aaagcatgat taaagtaatc gacaacagat gcgtcgattg
     8761 ttgacagtcc cagttctttt gcgtcgagaa cttcaatacg ttttgcgtat ttcttaagga
     8821 atgtaagagc tctttcatct aattctctcg ttgttccttc tgaaatctgg atcataaacg
     8881 ggcggtttgc atctgtgatc tcaaatggac cgtggaaata ctctcctgta tgaatgctgc
     8941 ttgaatcaag ccactgcatt tccataaaaa tacagatact ttccatataa gcagctccgt
     9001 aagaagcgcc gcttcccatt gtgtagatta ccgaatcatt cttatattct tctgcaaaag
     9061 cttccgctct ttctgctaca tgtttgcggg catttttaat aatgttgctg atcatgccaa
     9121 gtccttcata aaacttttca tagttctcat agccttctgt ctgcgctaac agttcaacag
     9181 ctacgagcag cgcgcactgt gttttttctc ctgcataatc gatgtctccc gcaaggtgat
     9241 ccaaacttgc atcaaatgta taacgaataa tgtaatctcc gaactcaatg atctcagatt
     9301 cttcaagcca tgtaaggata ataactgcgg cgcctttttc ttttccgagt tttgccgcct
     9361 gaatcgtctc aggtgtattt cctttatgac atgccagaca tatgattgag ttttctccga
     9421 aatccttcgg tgtactatga acaaactcgt tactgttgat ccatgctgaa cgaagtacct
     9481 gcgcctctct ttccataaat gtctttgccg gataaagcgc tcccagagaa cctccgcatc
     9541 ctacgaaata aatatttttt actccgcctt tttctttttt gttttcgata atttccgcaa
     9601 tgattttctt aatatctgta cttacatatg acattgtatt acatctcctt ctcgttgtgt
     9661 atttctttgt ttctacctag agtgtaatac aatataatac ttattgtcaa tatataattt
     9721 attttctatt tattatatac atttttatgc aaatgtatta taatgtgaac agtaatactt
     9781 tatatgggag gttgtttatg gaattaaata atacgattgc aacgccacta tacaaacaat
     9841 tagaagaaaa gcttcagcat gagatcgaga caggcgaacg ccctgccgga agccgcctcc
     9901 ccacagaaaa cgaactaagc gaaacttacc atgtaagcag agttactgtt cggaaagcgc
     9961 ttgccgaatt atccgaatta ggatatctcg accgcaaatc aggaaaagga acttttgtcg
    10021 cagaaaagaa gctccaccgc agtataacgg aagtcatcag ctttactgat atgtgccgta
    10081 tgatgaacgc cactgccgga gcaaaaacga tcaaaatcgc attagaagag ccttccgaac
    10141 gtgaaataaa agaaatgtct ttaaaacccg gagagaaaat acttgtattg gaaagaatca
    10201 gatatatgga tgatgagccg gtaattatcg aactcaacaa atttcccgaa tcattttcct
    10261 tcctgttcgg cgaagactta aacgatactt ctttgtttga agttctgaaa aaacataata
    10321 ttattttaga tcactcacga aaaactttag atatcatctt cgcttctcct caggaagcaa
    10381 aggcgcttaa tattaataaa ggttatccgc ttcttcggat caaaagcacg atccacgatg
    10441 ttgagaacac gattacaaac ttatgtcagc agttatgtat cggtgataaa tttactttta
    10501 ttgtctgact ttacatttgg acatttaatt atgctcccca ttaaacagtt cttatcataa
    10561 aaatctgttt gatgaggagc attttttaac ttatgtctga tttctattta cactttacac
    10621 ttggacttcc gcatctgtac actatgctta cactctatga ctgtacttac gttctgtccc
    10681 aacatttctt tttatcttct tccgtaatcg gacggatcac cttacatgga tttcctacag
    10741 ccacgctgtc cgaaggaata tctttcacga ctacgcttcc tgcgccgatc actacgttgc
    10801 ttcctatcgt tactccgggc atcacctgta cacctgcgcc aaaccatacg ttatttccta
    10861 ctttaattgg atatgcatat tccagacccc gattcctcct ctcgaaatca atcggatgtc
    10921 ccgatgtatg gaatccgcaa ttcggagcaa cgaacacatt atccccgaaa gcaactccgc
    10981 ctgcatcaag cacaaccaat ccgtgatttg cataaaagtt ttcacctgcc gatatacaat
    11041 atccgtaatc gcaccaaaag tcaggttcaa tgtgaatttt ttctcccgtt tttcccagga
    11101 tcttttttat taaccgactt cttttttccg tctcttttat cggcagatga ttatattcct
    11161 gacataacgc cttacactct attctttccc tttccaattc agagtcataa tttgcatcat
    11221 atagtttttg agactgcatt ttttcttttt ccgtcattac cttcttctcc ttcccatcgc
    11281 tgtaaaaatt atagctgttt ttatattcga tgtctatcgc ccctgtgaaa aatagggtga
    11341 atatttttct ctatatctcc tctatgtaaa tattgatcgc aagatgtata aacgtatgat
    11401 attcccgcag atcgacgccg gtaatctctt caattttatt gattcgatat aaaaacgaat
    11461 tacgatggat atgcagttat ttaatggcct ttcattttca caatgtaatc actttagcat
    11521 caatctcctt ttgaaaagta acatcatgct ccacgagaag cattgcagga gaaaactgcc
    11581 tgatcagctg ctcgatctgg attcttgaat acacatcgat atagtttaac ggttcatccc
    11641 atacatataa atgcgccggt tcacaaagac ttttggcaat caatactttc ttcttctgcc
    11701 ctgccgaaaa atctttcata tccttttcaa actgcacacg ttcaaaatcc attttcctca
    11761 atatcgcttt aaacagactt tcatctattt tatgaacctt agcaaactcg gtcaatgttc
    11821 cttttaaatg tgacgtatcc tgcggaacat aagaaatgac aagtcctgat ccactcgtaa
    11881 cagttccggt atgttcgatc ggatatttta aaagcaattt caataaactg ctttttccgc
    11941 ttccgttttt tccttgaata agaatacgct caccccggca tatctgaaat gtaaccgcat
    12001 cacacacttg atttccttca taaataaccg ctacatcaga aaaagatacc agtttttcac
    12061 tatgatacat aagcggagtt attttcaaca tttctgcata ttctgcatta ttaagcagtt
    12121 ttgatttttc ttcggctgct ctttcttttc ttgcctcggc atttttagat cttttcatca
    12181 tctttgccgc tttatgccct acataccctt tatccgccgc gcctctcttt gacgactcca
    12241 ccttatcaga ccatactttt gccttccttg cagattcttt catccgacga atatctttct
    12301 tcaaactttc atttctgttt atctcaaaat tctgcctctt cttaaagttc tccatccaag
    12361 aagaaaaatt tccgctttgt atttctatat ttgatctgtt taatgacaaa atgtgatcca
    12421 cacactcgtc cagaagataa cggtcatgtg aaacaaggat aaatcccctt ttccttttca
    12481 gataatctgc catctgtctg cgcccttcca catctaaatg atttgtaggt tcatcgatca
    12541 acagaaaatg ctcttctttt aaaaagagcg ccgcaagaag cgccttcgtc tgctccccgt
    12601 ttgacattgt ttcaaacggc ctccataaca cttcagactc tattccaagg taggacagtt
    12661 ctcttattaa ttcccattgc cgggcattcg gacatacttg acgcaaaata tcttctgtca
    12721 tctgcgtttt atccgaaaca ggaaatggaa aataatcaaa cgtaacagcg cctgttattt
    12781 ttcctctata ctcatactct ccaagaagaa gtttcagcaa agttgttttt cctcttccgt
    12841 ttcttcctac aagtccgagt ttccattctg tatcaatctg aaaactcaca ttttcaaaaa
    12901 caggatcaaa acttgaagga tatgaaaacg taatattttc aacacggatc atcgacataa
    12961 taaattcctc ctccgtttta caatctatct gccgcacaga atcaccggaa gcggccggca
    13021 agatcccgga caggtccgga ctttgtcttg tcgacatcaa aaaggctttt ttgcatacgc
    13081 aaaaaa
//
LOCUS       DBMDXF010000034        13074 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1382_length_13074_cov_2.413937, whole genome shotgun sequence.
ACCESSION   DBMDXF010000034 DBMDXF010000000
VERSION     DBMDXF010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13074)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_13074_cov_2.413937"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            229..1092
                     /gene="fba"
                     /locus_tag="ACZPD4_02550"
     CDS             229..1092
                     /gene="fba"
                     /locus_tag="ACZPD4_02550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1377992.1"
                     /translation="MLVSATEMLQKAKAGHYAVGQFNINNLEWTKAILLTAQECNSPV
                     ILGVSEGAGKYMTGYKTVVGMVNGMLEELGITVPVALHLDHGSYEGCMKCIEAGFSSI
                     MFDGSHYPIEENVEKTKELVKIAHEKNMSIEAEVGSIGGEEDGVIGKGECADPQECKM
                     VSDLGIDFLAAGIGNIHGKYPENWEGLSFETLDAIQQLTGDMPLVLHGGTGIPEDMIK
                     KAISLGVAKINVNTECQLAFAAATREYVEAGKDLEGKGFDPRKLLKPGTDAIMATVKE
                     KMELFGSIGQA"
     gene            1440..3554
                     /locus_tag="ACZPD4_02555"
     CDS             1440..3554
                     /locus_tag="ACZPD4_02555"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1377993.1"
                     /translation="MSTEAINVAEIFGQNVFDDTVMQERLPKKIYKNLKHTIEEGREL
                     DLETADVIAHEMKEWAIEKGATHYTHWFLPLTGVTAEKHDSFISAPQPSGKVLMSFSG
                     KELIKGEPDASSFPSGGLRATFEARGYTVWDCTSPAFIREDAAGAILCIPTAFCSYTG
                     EALDQKTPLLRSMEALDTQAMRLLRLFGNTTSKKVTPSVGAEQEYFLVDSEKFLQRKD
                     LIYTGRTLFGAMPPKGQEMDDHYFGTIRQRIASFMKDVNEELWKVGVTAKTQHNEAAP
                     AQHELAPIYAKANIAVDHNQIVMQTLKRVASRHGMKCLLHEKPFAGVNGSGKHNNWSL
                     ITDDGINMLEPGKTPHENIQFLLVLTCVLKAVNRHAELLRESAADPGNDHRLGANEAP
                     PAIISVFLGEQLEDVIDQLVSTGEATHSLKGGKLETSVRTLPDLMKDATDRNRTSPFA
                     FTGNKFEFRMVGSRDSIASANIVLNTIVAEAFSEACDVMEQADDVQKAIHDLIKEYAI
                     ENQKIIFNGDGYSQAWVEEAQRRGLPNIKSMVESIPALVSDKSVELFEKFGVFTRTEL
                     ESRAEIRYENYSKALNIEARTMIDMGRKQFIPAIIKYTKTLADTVNSVKAAGVDASVQ
                     LATLDEITVLLKEASRAQAQLEKVTETASSMDDGPDKAAYYYETVCPAMEDLRAPIDK
                     LEMIVDKEAWPMPSYGDLIFEV"
     gene            3765..5804
                     /locus_tag="ACZPD4_02560"
     CDS             3765..5804
                     /locus_tag="ACZPD4_02560"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1377994.1"
                     /translation="MIGGHLLHGGDYNPEQWIEYPEILEEDLKLMNKANVNCVTLGVF
                     SWAELEPKEDVYTFEWLEEIIDKLRERKIQVVLATPSGGMPHWLTQKYPETLQVQADG
                     TVNLPGKRHNFCYSSPVMRRKVKQIDRALAQRFGKKENVILWHISNEFGGNFKDSTCH
                     CEKCQKKFREWLKNKYGTLDKLNASWWTGFWSHKYTDWDQIHSPSPQGECLTTALTLD
                     WKRFSSEQITDFCKMEADALREFSDLPTTTNMMGFFKGVDYNTLKNAVDIISWDNYPF
                     WHERKDEVPEAVYTSAGHALMRSLKREPFLLMESTPSSVSWRSHNPLKRPGMHMLSSM
                     QAVAHGADSVQYFQWRKSRGGYEKFHGAVVDHKNGSDTRTFREVTEVGKRLEHLSGGI
                     KTFLNRAKAAIVFDWENWWAVEDTSGPRQDLDYVKCVTDHYRAFWECGLDVDFVSMDD
                     DFSGYRLLAAPLNYMYKKGYSEKVRAFVEAGGTYVTTYFSGIVNETDLCFIGRHPLED
                     VLGVVSEEMDAPSKEFENCFDYNGKEYPAYTMCDIVHAKAKTEIYSVYKKDFYKGCPV
                     VTENAYGSGRAYYLSAESDQRFLSAFYKDVFIKAGLLKEASSADRILKRLPHGVTAAK
                     REDETGRKGLVFVMNFNDRQVRLEGIGKQIDAESGEVYENVLEMTPFSCTILKLK"
     gene            5932..7635
                     /locus_tag="ACZPD4_02565"
     CDS             5932..7635
                     /locus_tag="ACZPD4_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR1377995.1"
                     /translation="MQSAADLRSLLNRIDHRGYPAYKDTKGTYRFPGYVLSIDHVQGD
                     PFASPSKVSIHVEGKAAAFPSDLYCGKDQRIALQDELTRQFAKKADQFAFQAKGSGKS
                     GMIAVSRCGQEVLERTACRLDPNSGDIILRMEIGFPANGRTINARELEKILFQFVPEC
                     VKASLFYKNADAARLRKIIDLAEDQRYIRDQLPKLGLCAFVANGSILPRESGVSSKPM
                     RGAVKFQSPKEQEVTITLPHAGVISGMGIKRGITLIVGGGYHGKSTLLKALETGVYPH
                     VAGDGREYVITDDTAVKIRAEDGRSIKKTDISMFINDLPNGKDTVLFCTEDASGSTSQ
                     AANVIESMEAGTSLFLIDEDTSATNFMIRDELMQRVIHREMEPITPFIERIRELYEKQ
                     GISTVIVAGSSGAYFHIADTILQMDRYEPKDITGYAKKEAEAFPAISLSQEPARQPDF
                     KRCPIANADLKGNDRIKMKTMGKEAVMINKEMIDLRYVEQIVDSEQVTALGYCMRYAQ
                     KHLMNGKKDLRQVIAEMEKVIEKESLDALCESRSSIACMAMPRAQEIFACFDRYRKLK
                     L"
     gene            7694..8854
                     /locus_tag="ACZPD4_02570"
     CDS             7694..8854
                     /locus_tag="ACZPD4_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR1377996.1"
                     /translation="MKFIHLSDLHIGKHLYHYNMKEDQEHILEEVIGYTEKLRPDAVV
                     IAGDIYDKSVPSAEAVAVFDDFLTRLSSVSPQVSILIIAGNHDSAQRLDYASRLLGSR
                     GIYIAGKAPETEKEHLKKVVLKDEYGPVNFYLLPFLKPGYVRLLCENETPGSYTEAVD
                     AVLRREDIDFSERNVLVSHQFYTGNGETPETCDSELFSVGGIDNVDISAISRFDYAAL
                     GHLHKAQRVGAEHIRYCGTLLKYSVSEADLEKSLHLVTIGQKKEAVTIEKFPLHPLRD
                     VQKVRGCLSGILSSADPKKRDDYVSVTLTDEIDPYRPKEQLEKVYSRILEVRTDNERT
                     RKKLEFKDEDIRMENPLETFSDFYEEMHGRKMSLEEAEMMTQIFDDVKGEER"
     gene            8851..11484
                     /locus_tag="ACZPD4_02575"
     CDS             8851..11484
                     /locus_tag="ACZPD4_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1377997.1"
                     /translation="MRPIKLTMSAFGSYSGMETIDFTKIQGGLFLVTGDTGAGKTTIF
                     DAVTYALYDRTSGGRRDGNMMRSQYADDEAETFVEYTFAYRDGQYTIRRNPEYMRAGK
                     RKNADGKIRLVKETSKVSLIMPDGKEFQGKKREVDQKIEQIIGLDAGQFTQIAMIAQG
                     DFLRLLHAESKERKKIFSKIFQTQIYRDVQEQLKEQGKALYIQLKENEADIRREMERV
                     DADGSKVQKVWEDLISQEMPSAEAVTETLGRILKEGKEAYETMEAEETALRKESEQLH
                     LMIGKKEEINRIFGLLERTESEYKRLQEQSAEYELLKNHALLGERSEQARRLEVQALR
                     TKEDARRIKEEIVTLNEWMEEHSKRENRLKEDAEQAESEMDRIEPKLREQIVRLREIL
                     PRYAKVRKLKAEYEKQTVQMTRCIDQCKAASEDYEEKYRIFFGEQAGILAQELKEGSP
                     CPVCGSVHHPHKAKISEGGVDEKTVEEAKKIRDEAERKRAAAQEKYQEVRAQFETEEK
                     ALFGERYSREDEKQAEDRLAEYESLLAQKRSNLKKLQDAYRKTAEENQRQAGRAENLV
                     LQKTELEERFEQEQEMFRQEIRKQRFADQEEYREAKKWIEGWRQMNDQVKTYESLVVQ
                     KKSSVETLKGQLNGKRRQETESDVRKKNETDMLLKEKNVRKLEIHGKNITHQSACDSL
                     KKYFAAEEELREKYEVVGNLSRTANGSLTGSVKLDFETYVQRKYFRQIIQAANRRLAK
                     MTSGGFILQCRELKDLSSQGQAGLDLDVYDLVTDSVRDVKSLSGGESFMAALSMALGL
                     ADIIQNTAGAVSLETMFVDEGFGSLDDQSRERAIRILKELAGEKGLVGIISHVNELKE
                     QIDWKLTVTKSGHGSHARWII"
     gene            complement(11490..12836)
                     /locus_tag="ACZPD4_02580"
     CDS             complement(11490..12836)
                     /locus_tag="ACZPD4_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1377998.1"
                     /translation="MWAYNETFYQIYPIGFCGAPTHNDGVTMHRILKIADWAGYLEEL
                     GIGSIILNPIFESDNHGYDTRDLTKIDCRLGTNEDFQTVCETLHSHNIKIMLDGVFNH
                     VGRGFWAFQDVLEKKWDSPYKDWFYINFDGNSAYNDGFWYEGWEGHYELVKLNLQNPA
                     VTDHLLSCIKMWIETFGIDGLRLDVAYSLDHNFMRRLRSFCEEIKPGFALVGEVLFGD
                     YNLIVNNEMLHSCTNYECYKGLYSSFNSMNLFEIAHSLNRQYGPEQWCIYRGKHLMTF
                     ADNHDVTRLASILTNKRHIPLAYGLLFGMPGIPCIYYGSEWGEEGTKSPDNDYALRPC
                     FDAPKPNDLTDLIRRLIALRRESDALCSGSYRNIVITNHQLIFERKTEKEQFLVAVNA
                     SDCEFTAGHHELNGTYEQILSFENETITSGKDTSVQELNGQLVLPAYSIRYLKRAL"
BASE COUNT         3982 a         2354 c         3566 g         3172 t
ORIGIN      
        1 gattatggat attttcaaaa tataaaaaaa gaaaacagta ttgtaaaatc gttatgagag
       61 cgttatttga aatttcattc gtgacgtctg taatataaat cgaattttga ttgtcttttt
      121 ttaagagata agctataatg aacgcagagc ttaaattaac attttcggtc agatgcagct
      181 tgcttatgag atgtatctgc gcggagaaga gaattggagg aaaacaatat gttagtatca
      241 gcaacagaaa tgcttcaaaa agcaaaagca ggacattatg cagtaggaca gttcaatatc
      301 aacaaccttg agtggacaaa agcaattctt cttacagcac aggagtgcaa ctctccggta
      361 atcctcggtg tatctgaagg agccggaaaa tatatgacag gatataagac agttgtaggc
      421 atggtaaacg gaatgctgga agaactcgga atcacagttc cggttgctct gcatcttgat
      481 cacggcagct acgaaggatg tatgaagtgt atcgaagcag gattctcatc tatcatgttc
      541 gatggatcac attatccgat cgaggagaac gtagagaaga caaaagagct cgttaagatc
      601 gctcatgaga agaacatgtc catcgaggca gaagtcggtt ctatcggcgg agaggaagac
      661 ggagtaatcg gaaaaggcga gtgtgccgat ccgcaggagt gtaagatggt ttccgatctc
      721 ggaatcgatt tccttgcagc aggtatcgga aatatccacg gaaaatatcc ggagaactgg
      781 gaaggattaa gttttgagac tctggatgcg atccagcagc ttacaggcga tatgccgctc
      841 gttcttcacg gaggcacagg tattccggaa gatatgatca aaaaggcaat cagcctcggc
      901 gttgctaaga ttaatgtaaa tacagagtgt cagcttgcgt ttgcggcagc tacacgcgag
      961 tatgttgaag caggaaaaga ccttgaagga aaaggatttg acccgcgtaa acttcttaaa
     1021 ccgggtacag acgcaatcat ggctactgta aaagagaaaa tggaattatt cggttctatc
     1081 ggacaagctt aagcagcggc gcagcatatc tgacgtctgc tttcggcggc tgaatttttc
     1141 aaaaaaatac ttgccttttt tagttggcgg agttatactg actgacagag acatgaacag
     1201 gggcgttcca gcatagcatg gagtgccctt tttgtaatag caacgagcta aagcacggga
     1261 gtgccagaga tcttggcggc tgcttacgat cccggaatgt tctcttcggt gcttccggtg
     1321 atttatggta tgccataatc ttttttgtat tatattgtaa aagcagtaat acatagagta
     1381 gtctgataca aaaaagcgtt catgcagtct tgttaaaagg taggaaggaa aggtgaatta
     1441 tgagtacaga agcaatcaat gtagctgaaa tttttggaca gaatgttttt gacgatacag
     1501 tgatgcaaga gcgtctgccg aaaaagattt acaaaaatct gaagcacacg atcgaagagg
     1561 gcagggaact tgatctggag acggccgatg tcatagctca tgagatgaaa gagtgggcga
     1621 ttgaaaaagg cgcgacgcat tacacacact ggttcctgcc tttaacaggc gttacggcag
     1681 aaaagcatga ttcatttata tcggctcccc agccaagcgg aaaagttctt atgagttttt
     1741 cgggaaaaga attgatcaaa ggagaaccgg atgcttcctc gtttccgtca ggaggattaa
     1801 gagctacgtt tgaggcaaga gggtatacag tgtgggattg tacttctccg gcatttataa
     1861 gagaagacgc ggcaggcgcg atcctgtgca tccctactgc attttgttct tataccggag
     1921 aggcgcttga tcagaaaaca ccgcttcttc gttcgatgga agcgctcgat acgcaggcaa
     1981 tgcgtctgct tcgcttattc ggaaatacaa cttcaaagaa agtgacgccg tctgtcggtg
     2041 cggaacagga atatttcctt gtagattcag aaaagtttct tcagagaaaa gacctgatct
     2101 atacaggaag gacgctgttt ggcgcgatgc ctccgaaagg tcaggagatg gacgatcact
     2161 atttcggaac gatcagacag agaatcgcat cgtttatgaa agatgtaaac gaagaacttt
     2221 ggaaggtagg cgttacggcg aaaactcagc ataacgaggc tgcgccggct cagcatgagc
     2281 ttgctccgat ctatgcaaaa gcgaatattg cggtagacca taatcagatc gtgatgcaga
     2341 ctttgaaacg ggtggcaagc aggcacggaa tgaaatgtct gctccatgag aaaccgtttg
     2401 caggggtgaa tggatcggga aaacataata actggtcgct gataacagac gatggcatca
     2461 atatgctcga accgggaaag acaccgcatg agaatattca gttccttctt gttctgacat
     2521 gtgttttgaa agcggtcaac agacacgcgg aacttctcag agaatctgcg gctgatccgg
     2581 gaaatgatca ccggcttggg gcaaatgaag cgcctccggc aattatttcc gttttccttg
     2641 gagagcagct cgaggatgtg atcgatcagc ttgtaagtac aggggaggcg acacatagtt
     2701 tgaaaggcgg aaagctggag acgagtgtgc gtactcttcc tgatctgatg aaagatgcga
     2761 cagaccggaa cagaacttct ccgtttgcat ttacgggaaa taaatttgaa ttccgtatgg
     2821 taggttcgag agactcgatc gcatctgcca atattgtgct caatacgatc gtggcggaag
     2881 cgttttcgga agcatgtgat gttatggaac aggcggatga cgttcaaaaa gcgatccatg
     2941 acctgatcaa ggaatatgcg atcgagaacc agaagatcat ttttaacgga gacggatatt
     3001 cacaggcatg ggtagaagaa gcgcaaagaa gaggacttcc aaacattaag tctatggttg
     3061 agtcgatccc ggcgcttgta tctgataagt cggtagaact ttttgagaaa ttcggagtat
     3121 ttacacgcac agagcttgaa tcccgcgcgg aaattcgcta tgaaaattat tcaaaggcgc
     3181 tcaatatcga ggctcgtacg atgatcgata tgggaagaaa acagtttatt ccggcgatca
     3241 ttaaatatac aaagacgctg gcggatacgg ttaattcggt aaaagccgca ggtgtggatg
     3301 ccagtgttca gcttgcgacg cttgatgaga ttaccgtgtt gctcaaagaa gcaagccgcg
     3361 ctcaggcaca gcttgagaaa gtgacggaga cggcttcttc catggacgac ggtcctgata
     3421 aagcggctta ttattatgag acagtgtgcc ctgcaatgga ggatctgcgt gcgccgatcg
     3481 acaagctgga gatgatcgtt gataaagagg catggccgat gccgtcttat ggagatctga
     3541 tatttgaagt ataattagaa acaatgttct tgcgaacgat gtttcgataa agtggaaaca
     3601 gagatttttt gagatatgat tccgaaagcc tctgtttctt tttataattg acatctgaaa
     3661 aatctcgggg catacttatt gcagaaagaa gagctggaga ctgcattatt accggaatgc
     3721 ggcaaaaaca cacattgtcc gaaatgaaaa caggaggttg tactatgata ggaggacact
     3781 tattacacgg tggagactat aatccggaac agtggatcga atatccggag atattggaag
     3841 aagatttaaa gttgatgaat aaggcaaatg taaactgtgt gacgctcgga gtgttttctt
     3901 gggcggagtt agagcctaag gaggacgttt atacgtttga atggctggag gaaattatag
     3961 ataaactccg agagaggaag atacaggtag ttttggcgac tccgtcggga ggaatgccgc
     4021 actggcttac acaaaagtat cccgagacgc ttcaggtgca ggcggacggt acggtaaacc
     4081 tgccggggaa gcgccataac ttctgctatt cctctccggt catgaggcgg aaggtaaagc
     4141 agatagaccg ggctcttgcg cagcgattcg gaaagaaaga aaatgtgatc ctctggcata
     4201 tttccaatga gttcggagga aacttcaaag attccacatg tcattgtgaa aaatgtcaga
     4261 aaaagtttcg ggaatggctg aaaaacaagt acggaacact ggataagctt aatgcgtcgt
     4321 ggtggacggg attttggagt cacaaatata cagattggga tcagatccac agcccttctc
     4381 cgcagggcga atgtctgacg acagcgttga ctttagattg gaagagattt tcttccgagc
     4441 agattacaga tttttgcaaa atggaagccg atgcattgag agaattttcc gatcttccga
     4501 caacgacgaa tatgatggga tttttcaaag gagttgatta caatactctg aaaaatgcag
     4561 ttgatatcat atcatgggat aattatccgt tttggcatga gagaaaagat gaagtgccgg
     4621 aggcggtata tacatctgcg ggtcatgctc ttatgagaag tctgaaaaga gagccgtttc
     4681 ttttaatgga aagcacgccc tcttctgtga gctggcgttc tcacaatccg ctgaaaagac
     4741 cggggatgca catgctttct tctatgcagg cagttgcgca cggagcagat tcggttcagt
     4801 attttcagtg gagaaagagc aggggcggct atgagaaatt tcacggagca gtcgtagatc
     4861 ataagaacgg ttctgatacg agaacatttc gagaagtgac ggaagtagga aagcggctgg
     4921 agcacttaag cggcgggatc aaaacttttt taaacagggc gaaagcggcc attgtttttg
     4981 actgggaaaa ctggtgggct gtagaagaca cgagcggacc gcgtcaggat cttgattatg
     5041 tgaaatgtgt gacggatcac taccgtgcgt tttgggagtg tggcttggac gttgattttg
     5101 taagtatgga cgatgatttt tcgggatatc ggcttctggc ggcgccactt aattatatgt
     5161 ataaaaaggg atattccgaa aaggtcagag cgttcgtgga agcgggcgga acttatgtga
     5221 caacgtattt cagcgggatt gtaaatgaaa ccgatctttg ttttatagga cggcatccgt
     5281 tggaagatgt actcggggtg gtttctgaag aaatggacgc gccgtcaaag gagtttgaga
     5341 attgttttga ttataacgga aaagaatatc cggcgtatac gatgtgtgat atcgttcatg
     5401 caaaagctaa gacggaaatt tactctgttt ataaaaaaga cttttataaa ggatgtccgg
     5461 tagtaactga aaatgcatat ggaagcggaa gggcgtatta tctttcggca gaatcggatc
     5521 agaggtttct aagtgcattt tataaagatg tgtttataaa ggcgggattg ttgaaagaag
     5581 catcatctgc cgaccgcata ttaaaacgac ttccccatgg cgtgacggct gcaaaaagag
     5641 aggatgagac aggaagaaaa ggtcttgttt ttgtgatgaa ttttaatgac aggcaggttc
     5701 gtttggaagg cattggaaag cagattgatg cagaaagtgg agaagtatat gaaaatgtgt
     5761 tggaaatgac cccgttttca tgtacgattt taaagttgaa gtaaagcgtc agtatgaata
     5821 ttgtcaggca gtgaaatcag aacattaaga taacggcaga cagagtaaaa gaacgtaaaa
     5881 cgtaagccga tgcgcggcgc gggaaaatag tggaagaaag gaatcgagaa aatgcagtca
     5941 gcagcagatt tgagaagttt attgaacagg atcgatcatc gtggatatcc tgcttataaa
     6001 gatacgaaag gaacatatcg gtttccggga tatgtgttgt ctatagatca tgtacaggga
     6061 gatccgtttg cgtctccgtc aaaggtgagc atacatgtag aaggaaaggc agcggcattt
     6121 ccgagtgatc tttactgtgg gaaagatcag cggatcgcgc ttcaggatga attgacgcgt
     6181 cagtttgcaa aaaaggcgga tcagtttgct tttcaggcaa aaggttcggg aaaaagtggg
     6241 atgatcgctg taagccgctg cgggcaggaa gttttggaac gtacggcctg cagacttgat
     6301 ccgaacagtg gagatatcat tttgcggatg gaaatcggtt ttcccgcaaa cggaagaacg
     6361 atcaatgcgc gggagcttga gaagatcctg tttcagtttg tgccggaatg tgtaaaggcc
     6421 tctttgtttt ataaaaatgc agatgcggcc cgtctgcgta aaattataga tcttgcggaa
     6481 gatcagaggt atataagaga tcagcttccg aaactcggac tttgcgcttt tgtggcaaac
     6541 ggaagcattc ttccgagaga gtccggcgtt tcgtctaagc cgatgcgcgg cgcagtaaag
     6601 tttcagtctc caaaggagca ggaagtgacg attacacttc cgcatgcggg agtcataagc
     6661 ggaatgggga tcaaaagagg gatcacgctt attgtgggcg ggggatacca cggaaaatct
     6721 acgctgttaa aggcgttgga aacaggagtt tatccgcatg ttgcaggtga cggacgggag
     6781 tatgtgatca ccgacgatac ggcggtaaag attcgcgccg aggacgggcg gagtattaaa
     6841 aagacggata tttctatgtt tatcaacgat cttccaaatg gaaaggacac ggttttattt
     6901 tgtacggaag atgccagtgg aagtacgtct caggcggcaa atgtaatcga aagcatggaa
     6961 gccggaactt ctcttttttt gatcgatgag gacacaagcg ctacgaattt tatgatacgg
     7021 gatgaactga tgcaaagagt gattcatcgg gagatggagc cgatcacgcc gtttatcgag
     7081 cggataagag aattgtatga aaaacagggg atttccaccg tgatcgtagc gggaagttca
     7141 ggagcttatt tccatattgc agatacgatc cttcagatgg atcgatacga gccgaaagat
     7201 attaccggat atgcgaaaaa ggaagcggaa gcatttcctg cgatcagtct ttctcaagag
     7261 ccggcgcggc agcctgattt taagagatgt ccgatcgcaa atgcagattt aaaggggaat
     7321 gaccggatca agatgaagac gatgggaaaa gaagctgtca tgatcaacaa agaaatgatc
     7381 gatcttcgct atgtggaaca gattgttgac agcgaacagg tgacagcgct cggatattgt
     7441 atgcgatatg ctcaaaaaca tcttatgaac ggaaagaaag atcttcgtca agtgatcgca
     7501 gagatggaaa aagttataga aaaagagtcg cttgatgcgc tctgtgagag cagatcaagt
     7561 atcgcatgta tggcgatgcc aagagcgcag gaaatttttg cctgctttga cagatacaga
     7621 aaactgaaac tttaataagc gtgttttcgc agaaaaagaa aacgatcagg atgaagcgga
     7681 aaggaacagt aaaatgaaat ttatacattt atcggatctg catatcggaa aacatcttta
     7741 tcattacaat atgaaagaag atcaggaaca cattttggag gaagtgatcg gatatacaga
     7801 gaagcttcgg ccggatgcgg ttgtgatcgc gggagatatt tatgataaat ctgttccgtc
     7861 ggcagaggcg gtcgcagtgt ttgacgattt tctgacacgg ctttcatcgg taagccctca
     7921 ggtgtcgatt ttgatcatcg cggggaatca tgattcggcg caaagacttg attatgccag
     7981 ccgtctgctt ggaagccgcg gcatctatat tgcggggaaa gcgcctgaga cggaaaaaga
     8041 acatttgaaa aaagttgtat taaaagatga gtatggtccg gtgaattttt atctgcttcc
     8101 ttttttgaag ccgggatatg taaggttgtt gtgtgaaaac gagacgccgg gcagttatac
     8161 agaggctgtc gatgcggttt taaggagaga ggatatcgac ttttcggagc gaaacgtact
     8221 tgtatctcat cagttttata caggaaacgg cgagactccg gaaacgtgtg attcagaact
     8281 tttcagtgtc ggcggaattg ataatgtaga catttccgca atcagccgat ttgattatgc
     8341 ggcgcttgga catttgcaca aggcacagag agtgggggcg gaacatattc gatattgcgg
     8401 gacgcttttg aaatattcgg tcagtgaagc ggacttagaa aagtctcttc atcttgtgac
     8461 gatagggcag aaaaaagagg cggtcacaat cgaaaaattt ccgcttcatc cgctgcggga
     8521 cgtacagaaa gtgcggggat gtctttctgg aatcttaagt tcggcagatc cgaaaaagcg
     8581 ggatgattat gtgagcgtga cgcttacaga cgaaatcgat ccttatagac cgaaagaaca
     8641 gttggaaaaa gtgtacagcc gtattttaga agtgcggacg gataatgaac ggacgcgtaa
     8701 aaaacttgag tttaaggatg aggatatccg catggaaaat ccactggaaa cattttcgga
     8761 tttctatgaa gaaatgcatg gaagaaagat gtctttagaa gaggcggaaa tgatgacgca
     8821 gatttttgat gatgtgaaag gagaagaaag atgagaccga tcaaacttac gatgtctgcg
     8881 tttggttctt attccggtat ggaaacgatt gactttacga aaattcaagg cgggctgttt
     8941 ctcgttaccg gcgataccgg agccggaaaa acaacgatct tcgatgcggt gacatatgct
     9001 ctttacgaca ggacaagcgg cggacggcgc gacggaaata tgatgagaag ccagtatgcc
     9061 gacgacgagg cggaaacatt cgtggaatat acgtttgcat atcgggacgg gcagtatacg
     9121 atccgaagaa acccggaata tatgcgcgcg ggaaagcgga aaaatgcaga cggaaagatc
     9181 cgccttgtaa aggaaacgtc aaaagtcagc ctgatcatgc ccgacgggaa ggagtttcag
     9241 gggaaaaagc gtgaagttga tcagaaaata gagcagatca tcggattaga cgccgggcag
     9301 tttacgcaga tcgctatgat agcgcaggga gacttcctgc ggctgctgca tgcggaatca
     9361 aaagagcgaa agaagatatt ttccaagatt tttcagacgc agatatacag agacgtccag
     9421 gaacaattaa aagagcaagg aaaagcattg tatatacagt taaaagaaaa tgaagccgat
     9481 attcggcgtg agatggagcg cgtcgatgca gacggatcga aggtgcaaaa ggtgtgggaa
     9541 gatttgattt cccaggaaat gccctcggca gaggctgtaa cagaaacgct cggccggata
     9601 ttaaaagaag gaaaagaagc gtacgaaacg atggaagccg aagaaacggc gcttcggaaa
     9661 gaatcggagc agctccatct tatgatcggg aagaaagagg agatcaacag gatcttcggt
     9721 ctgttagagc gaacagaatc ggaatataaa agactgcagg aacaaagcgc ggaatatgag
     9781 cttttgaaaa atcacgcgct gttgggagaa cgttcggaac aggcgagaag attggaagtg
     9841 caggcgcttc gtacaaagga agatgcacgg aggatcaaag aagaaatcgt tactctgaat
     9901 gagtggatgg aagagcacag taagcgggag aacaggctga aagaagatgc ggagcaggcg
     9961 gagagtgaga tggatcggat cgaaccgaag cttcgagaac agatcgtgcg tttgcgtgag
    10021 attcttccca gatatgcaaa agtacggaaa ttaaaagcag agtatgagaa acagacagtt
    10081 cagatgacac gctgcataga tcagtgtaaa gcggcgtcgg aagattatga agaaaaatac
    10141 agaatctttt tcggcgaaca ggcggggatt cttgcacaag aacttaagga aggttctccg
    10201 tgtcctgtgt gcgggtcggt gcatcatccg cataaggcga aaatttccga aggcggagtc
    10261 gatgaaaaga cagtagaaga ggcgaaaaag atccgtgatg aagcggagcg aaagagagcg
    10321 gcggcacagg aaaaatatca ggaagttcgc gcacaatttg aaacagaaga aaaagcgctt
    10381 tttggagaaa ggtattctcg ggaagatgaa aaacaggcgg aagaccggct tgcagaatat
    10441 gagagcctgc ttgcgcaaaa aagatcgaat ttgaaaaagc ttcaggacgc atacagaaaa
    10501 actgcagaag aaaaccaacg tcaggcaggg cgggcggaaa atctggtttt acagaaaacg
    10561 gaactggaag agcggttcga gcaggaacag gaaatgtttc gtcaggagat acgaaagcag
    10621 cggtttgcgg atcaggagga ataccgggag gcgaaaaaat ggatcgaagg ctggaggcag
    10681 atgaacgatc aagtaaagac gtatgaaagt ctggtcgttc aaaagaaaag cagtgttgag
    10741 acattgaaag gtcagttaaa cggtaaaagg cggcaggaga cggaaagtga cgtcagaaag
    10801 aaaaatgaga cagatatgct tttgaaagaa aagaatgtgc gaaaactgga gattcacggg
    10861 aaaaatatta cccatcaaag cgcctgtgac agccttaaaa agtattttgc ggcagaggag
    10921 gaacttcggg aaaagtatga agttgtcggt aatctgagca ggacggcgaa cggaagtttg
    10981 acaggcagtg tgaagcttga ttttgagacg tatgttcaga gaaagtattt ccggcagatc
    11041 atacaagcgg cgaaccgaag gcttgccaaa atgacttccg gaggatttat cctgcagtgt
    11101 cgggaattga aagatttaag cagtcagggg caggccggat tagatctgga tgtgtacgat
    11161 ctggtgacag attctgtccg cgacgtaaaa tctctgtcag gcggagaatc atttatggcg
    11221 gcgttgtcaa tggcgttagg acttgcggat atcattcaga acacagccgg agcggtgagt
    11281 ttggagacga tgttcgtaga cgaaggattc ggttctttgg atgatcagtc gcgggagagg
    11341 gcgatccgta tcttgaaaga acttgcagga gaaaaaggac ttgtaggtat catttctcat
    11401 gtcaatgaat tgaaagagca gatcgactgg aagctgactg tgacaaaaag cggccatgga
    11461 agccatgccc gctggatcat ataagacttt tataaagctc tttttaaata ccggatgctg
    11521 tatgcgggca ggacgagctg tccgttaagt tcctgtacgg aagtgtcctt gccgcttgtg
    11581 atcgtttcgt tttcaaagga aaggatctgt tcatatgtgc cgtttaactc atggtgtccg
    11641 gctgtgaatt cacagtcgga tgcgttgacg gcaacaagga attgctcttt ttctgttttg
    11701 cgctcgaaaa taagctgatg atttgtaata acgatatttc gataagaacc gctgcacagc
    11761 gcatcgcttt ctctgcgaag agcgatcagg cggcggatca gatcggtgag atcgtttggc
    11821 ttaggcgcat cgaaacaagg ccttaaggca taatcattat caggtgattt cgttccttcc
    11881 tctccccatt cgcttccgta ataaatacag ggaatacccg gcataccgaa caaaagtccg
    11941 taagcaaggg ggatatgccg cttgtttgtc aatatggaag caagccgcgt gacgtcatgg
    12001 ttatctgcaa aagtcatgag atgctttcct ctgtaaatgc accactgctc cggtccgtat
    12061 tggcggttta atgagtgggc gatctcgaac aagttcatgc tgttaaagct ggaatataat
    12121 cctttgtaac attcgtagtt cgtgcagctg tgaagcattt cgttgttgac gatcaggttg
    12181 tagtctccga ataaaacttc tcctacaaga gcgaatcccg gcttaatctc ttcgcagaaa
    12241 gagcggaggc ggcgcatgaa gttatgatcg agagaataag cgacatcaag gcgcagtccg
    12301 tcaatgccga aagtttcgat ccacattttt atgcagctaa gcagatggtc tgtgacggca
    12361 ggattttgca ggtttaactt cacaagttcg tagtgtcctt cccagccttc ataccagaag
    12421 ccgtcgttat atgcgctgtt gccgtcaaaa ttaatataaa accagtcttt atacggagaa
    12481 tcccatttct tctctaacac atcctgaaat gcccaaaaac ctctgccgac gtgattgaat
    12541 accccgtcga gcatgatctt gatattgtgc gaatgaagcg tttcacatac tgtttggaaa
    12601 tcttcgttcg ttccgaggcg acagtcgatc ttcgtaagat ctcttgtgtc atagccgtga
    12661 ttatctgatt caaaaatcgg atttagaatg atcgatccga tcccgagctc ttcgagatat
    12721 ccggcccagt ctgcaatttt caaaatccgg tgcattgtca cgccgtcatt gtgcgtcggc
    12781 gcgccgcaga agccgatcgg ataaatctga taaaaagttt cattgtaagc ccacataatt
    12841 tcagtccttt cagttattat atttttgctg tgatcagata tatgtttcct cgtaaacgga
    12901 catctgtaca gccaactgtt gataggtaga ttgtaacata tcaaggcaga attgacatta
    12961 aaaagtgtag tagatatact gaataaaggc acaaaaacaa agataaagga agtaaaaacg
    13021 gtaaaaaatg agtttgatgg aaaggatccc caaagatttt tataaattat tttc
//
LOCUS       DBMDXF010000035        12996 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1395_length_12996_cov_2.447305, whole genome shotgun sequence.
ACCESSION   DBMDXF010000035 DBMDXF010000000
VERSION     DBMDXF010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12996)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1395_length_12996_cov_2.447305"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(274..346)
                     /locus_tag="ACZPD4_02585"
     tRNA            complement(274..346)
                     /locus_tag="ACZPD4_02585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Lys,seq:ctt)
     gene            complement(878..1528)
                     /gene="trmB"
                     /locus_tag="ACZPD4_02590"
     CDS             complement(878..1528)
                     /gene="trmB"
                     /locus_tag="ACZPD4_02590"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1377999.1"
                     /translation="MRLRNIPRADDTLQAHFTVIKNPKEFRGNWTSVFGNDRPIRIEI
                     GMGKGRFLLNMAKTYPNINFIGIERYSSVLLRAVEKFDTEEFSDLPNIRFVCMDAREL
                     PEVFAESEVDRIYLNFSDPWPKSRHAKRRLTSTEFLSRYEKVLPPQGLVEFKTDNTPL
                     FDFSLEQVKEAGWTLQAFTYDLHHNKELNKENIMTEYEEKFSSKGNPINKLIAMRK"
     gene            1756..2067
                     /locus_tag="ACZPD4_02595"
     CDS             1756..2067
                     /locus_tag="ACZPD4_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378000.1"
                     /translation="MDAILDKLTEIENAASAIVRHAEEQKDVLGQEFDEKRKKFDEDL
                     ENKTKARLEKIREQLEKEQSRVLNSSSGASEDTICSLRKEYEEKHTEYAHEILRRITE
                     V"
     gene            2071..3117
                     /locus_tag="ACZPD4_02600"
     CDS             2071..3117
                     /locus_tag="ACZPD4_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000220 - vacuolar proton-transporting
                     V-type ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V0D/AC39 family V-type ATPase subunit"
                     /protein_id="HIR1378001.1"
                     /translation="MGNLLKYSGIVTKIRAMEAKLLTPEQFADIANLRSVPDIADYLR
                     KNTAYAEVLDTLTEDQIHRGNIEKVLVQSLYHDYTKIYRFCGQSQREFMKLIMKGYEI
                     ELINYCLRIVINHYKKPFDLNYKSAFFDRYSQISIGKLITARTTDELVENLKETEYYA
                     PLKKIKDTDNVTLYDYNLALDLYYYTSTWKEQKKILKKSDLELFMRDRGSKIDLLNIQ
                     WIYRAKKYYNMKPADIYLMLIPIHYKLSTELVKDMVEAPGVEEFENVVIRTTYARHYN
                     FKQNLTMEQMYADCLHHLYTADRRKNPYSVSAINTYLFLKEEELKKLTTVMECVRYGL
                     NPGETAAYIGGRTR"
     gene            3114..5063
                     /locus_tag="ACZPD4_02605"
     CDS             3114..5063
                     /locus_tag="ACZPD4_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1378002.1"
                     /translation="MIVKMKFINISGPRNDIDRVTDQYLSRYEIQLESALSELKTVDN
                     LRPFVELNPYREVLSKANEFVGYLPNAETVEPDTKLGLDDMFELVRKADEDYRTLQEK
                     KEKLKQKIEEYRAKQQIVAPFRPLECDLHRVLHYRYITYRFGRIAVEFYHKMQKYLMD
                     DLKAIFVEGERDESYVYGVYFVSQAETQKVDAVFRSLHFERIELKDEFEGTPDTAYRV
                     LENEIARVNLEIEDLDKQAGEMLADRAPQLVAARDRLEELSNNFDVRKLAARMEDKQE
                     DYYILCGWMAEDDVDKFVEEVKEDDKVFVVVEDDHDAYFGEPPTKLENPKLFKPFEMF
                     VSMYGLPAHNEMDPTMFVGLTYSFIFGVMFGDVGQGLLLMIGGGLVYKFKKAPLAGII
                     ATAGVFSTIFGFLFGSIFGFEDVLPALWIRPIDHMTTLPFLGKLNTVFIVAVAFGMFL
                     IMVAMVLHIINAVRGKNMESAWFDPNGAAGLVFYLAVVVTVVLFMTGNPLPGGIVMAI
                     MFGIPLLLIFFREPLTRMLQKRADKMETGKAMFVVQGFFELFETLLSYFSNTISFIRI
                     GAFAVSHAAIMEVVLQLAGAESGSPNWAGVIFGNLFVCGFEGLIVGIQVLRLEYYELF
                     SRFYKGSGHAFDPYAKRKTKKNSRK"
     gene            5102..5536
                     /locus_tag="ACZPD4_02610"
     CDS             5102..5536
                     /locus_tag="ACZPD4_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR1378003.1"
                     /translation="MTMGTKIVFIITLIVSIIVPFGYYLLGEKSRKRYKRAIGTNAFF
                     FFGAFAIASIMLLGGPNVQAAEAVTADAGFATGMGYLAAAIVTGLSCIGGGIAVASAA
                     SAALGAISEDSSVLGKSLIFVGLAEGVCLYGLIISFMILGNL"
     gene            5608..5916
                     /locus_tag="ACZPD4_02615"
     CDS             5608..5916
                     /locus_tag="ACZPD4_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1378004.1"
                     /translation="MKMYLISDNVDTYTGMRLAGVDGIVVHEQDELKQALERVLEDKS
                     IGIVLLTEKFGREFPAIIDTFRLERKMPLLVEIPDRHGTGRKKDFITSYITEAIGLKL
                     "
     gene            6030..6617
                     /locus_tag="ACZPD4_02620"
     CDS             6030..6617
                     /locus_tag="ACZPD4_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR1378005.1"
                     /translation="MTLEEKLTYMQTVSMEQARAEGNAIIDAHREALEKIFSDHKSEM
                     MRQAETRIKAETTNAKRTVSQALAKSQLEIKRSQGKVQQELKDKVFEEAMQLVNDYMK
                     TKAYDDFLVKCIRKAERFAGDDPVVIYINPSDEKKRTELEKETGAKLTVSAEDFIGGV
                     RAVIRSRNILMDHSFKTQIQDEYDKFLFLGGDGIA"
     gene            6610..8376
                     /locus_tag="ACZPD4_02625"
     CDS             6610..8376
                     /locus_tag="ACZPD4_02625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1378006.1"
                     /translation="MLNTGTIYGINGPVIYLKGNTGFRMSEMVYVGKQKLVGEVIALD
                     KDRTTIQVYEETTGLRPGEEVVASGNAVSVTLAPGILNNIFDGIERPLERIAETGGAF
                     ITRGVSVDSLDREKKWKTHIVVEKGQYLHGGDIIAEVPETHAILHKCMIPPDVEGTVK
                     EVVKDGEYTIDETLVILELPDGGEKKLTMTQRWPIRVPRPTHHRFPASVPLVTGQRII
                     DTMFPIAKGGTAAIPGGFGTGKTMTQHQIAKWADADIIIYIGCGERGNEMTQVLEEFG
                     ELTDPRTGNPLMDRTTLIANTSNMPVAAREASIYTGLTLAEYYRDMGYDVAIMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLASRLSVFYERAGMMHNLNGTDGSVTIIGAVSP
                     QGGDFSEPVTQNTKRFVRCFWGLDKSLAYSRHFPAIHWLTSYSEYLNDLSHWYQDNVS
                     PQFVDYRNRLMALLNQESSLLEIVKLIGSDVLPDDQKLVLEIARVIRLGFLQQNAFHK
                     DDTCVSMEKQFLMMDTILYLYKQARTLVTMGHPMSVLKAENIFDRVISIKYDVPNDRL
                     EMFAQYHRDIDAFYQNVLEKNA"
     gene            8392..9777
                     /locus_tag="ACZPD4_02630"
     CDS             8392..9777
                     /locus_tag="ACZPD4_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1378007.1"
                     /translation="MSIEYLGLSSINGPLIALEGVQDAFFDEIVEFVVDGTQRKIGRI
                     VEIYEDKAVIQVFEGSENMSLKNTHTKLTGHPMEIALSPDILGRTFNGIGNPIDGLGP
                     IASTLRRDVNGSPLNPVKREYPRNYIRTGISAIDGLTTLIRGQKLPIFSGNGLPHDQL
                     AAQIVKQASLGENSDEKFAIVFAAMGVKHDVADFFRNTFEESGVADHVAMFLNLANDP
                     VVERLITPKVALTAAEYLAFEQNMHILVILTDMTSFAEAMREVSSSKGEIPSRKGYPG
                     YLYSELATIYERAGIVQGVNGSVTQLPILTMPNDDITHPIPDLTGYITEGQIVLDRPL
                     NGQSIYPPINILPSLSRLMKDGIGEGYTREDHQDLANQLFSAYAKVGDARNLASVIGE
                     DELSPIDKKYLEFGKEFEEKYIGQGMNENRTMEETLDLGWKLLGRLPREELDRVDTKI
                     LDKYYKPSEEE"
     gene            9840..10469
                     /locus_tag="ACZPD4_02635"
     CDS             9840..10469
                     /locus_tag="ACZPD4_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1378008.1"
                     /translation="MDPRTFPTKGNLMLAKNSLALAKQGYDLMDKKRNILIRELMDLI
                     DEARTIQEEIDTTFTYAYECLQRANIENGISNVEMLAYTVPIENSITIQTRSIMGTEI
                     PHVKYMPDAGKPTYSFSTTRESIDKAREAFRKVKDLTVKLSMVENAAYRLAGNIKKTQ
                     KRANALQNITIPMYTSLVYTITNALEEKEREEFTRLKVIKRMKTKKQGG"
     gene            10557..11150
                     /locus_tag="ACZPD4_02640"
     CDS             10557..11150
                     /locus_tag="ACZPD4_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1378009.1"
                     /translation="MSNEEQKSITPGIYLWLIAVNIYISMFAFGGGYVVVPLVRRSFV
                     EKKKIFTEDELMEMSAVAQSSPGAIAVNLAALSGYKAAGTAGAVISCAASVIPPLVIL
                     GIISGFYDLFIANKIVAAVLKGMQSGVAALIVSVVADMCTVILKEKSFFLFSLIPIAF
                     IANFIFDVNVALILAVCCGLCILRVYVRSGGHKWKFF"
     gene            11135..11701
                     /locus_tag="ACZPD4_02645"
     CDS             11135..11701
                     /locus_tag="ACZPD4_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1378010.1"
                     /translation="MEILLKLFLTFLKIGLVSIGGGYAVIPSIHEEVVVKAGWIGEKV
                     FADIITISQMTPGPLAVNTSTFVGLQIAGAAGAVVATVGCVFCGVAISLTLYRFFQKH
                     KESVYIFETLNGLKAASLGLIVSAAATIILLSFFGTGDPDLKTMKESLDIGAVAIFGG
                     VLFLSRKRKINPIMLMLVSGAAGIILYM"
     gene            11794..12759
                     /locus_tag="ACZPD4_02650"
     CDS             11794..12759
                     /locus_tag="ACZPD4_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378011.1"
                     /translation="MREKKNKNDNVNDVNGKRDNKKAAGKFKNWTTALSMELREHKSS
                     AIVYFTLRALVIIMLVLQLLNRNYENVFLCILTLLLLVIPSLAQITFKVELPTALEII
                     ILVFIFSAEILGEISEFYLVFPFWDTVLHTLNGFLAAAIGFSLVDLLNRSDRTVFSLS
                     PLFTAIVAFCFSMTIGVVWEFFEFGMDMIMELDMQKDTVIHTIRSVMLDPGGHNVPYA
                     IQNITDVAVNGQSLGLGGYLDIGLLDTMQDLIVNFIGAAVFSVLGFFYVKSRGKGKVA
                     GSFIPRRKAHDRDFLRIARESSEVEQDIQEKSQKVPKGSAKNGDR"
BASE COUNT         3848 a         2277 c         3398 g         3473 t
ORIGIN      
        1 tgtaaaaaga cacaactgac agaaacgcag aaacaggcgg gagatataaa caaaaacgga
       61 actgttgata tcgaagactt gagaacaatc ctgagagttg tttgcggaaa agtagaaagc
      121 ctgtaagtga tgtaaatagt aaatcgataa gaaaagcgga tggtgattga gctgtccgct
      181 ttttgtaatg gggaagagac ggatatttga atttaaagta caaaaaaatc accgtccccg
      241 cggctctcaa gtccgcaaaa acagtgatta gaatgcgcga tcaggggttc gaaccctgga
      301 caccctgatt aagagtcagg tgctctacca actgagctaa tcgcgcatgc aatagtatgt
      361 tgttttacaa cgcaagaaag attataattt aaaatttatg aaaaatcaac cctttttaac
      421 aaaaaaattc gattttcttt attttttctt ttattttcgt ctttatttta ccgatttgtt
      481 tttacttttc atcttttgtt aaaaaatagt attgtttttg tctgtatttg cggttgtcac
      541 cggctgtgtg tacatgagaa taaaaagttg tttgtcagga gtgtttgcgg gaacagaagt
      601 ctgaatttaa gattttgtac acttcatcaa aagaattttt gataataaga agttttcggt
      661 taagtctcgg atgacagtag catatttcac agagacggta tttttgatgt tttcgtttca
      721 tataaactcc ttttaacgga acggattgag cggacagctt aaataagcga ggccggaaag
      781 cgtctcatac atctttctat attatatgaa aaaaacaggg agaggttcgt tggaagcgtc
      841 tcaccctgct ttttatagca tatccgtgct gccgccgtta ttttctcatt gcgatcagtt
      901 tattgatcgg gtttcctttt gaagaaaatt tttcttcata ttctgtcata atattttctt
      961 tgtttagctc tttgttgtga tgcagatcgt atgtaaaagc ctgaagcgtc catccggctt
     1021 cctttacctg ttccagagaa aagtcaaaaa gaggggtgtt gtccgttttg aactccacaa
     1081 gtccttgcgg tgggaggact ttttcataac gggataaaaa ctctgttgat gtaagacggc
     1141 gttttgcatg acgtgatttt ggccatgggt cggaaaaatt caggtaaatg cggtcgactt
     1201 ctgattcggc aaatacttcc ggcagttctc ttgcatccat gcagacaaag cggatattcg
     1261 gcaaatctga aaattcttcg gtgtcaaatt tttctactgc ccgaagcagg acgctggaat
     1321 atcgttcgat tccgataaag ttgatattag gataggtttt tgccatattt aataagaagc
     1381 gtccttttcc cattccgatc tcgatccgta tcggacggtc gtttccgaaa accgaagtcc
     1441 agtttccgcg gaactctttc ggattcttga ttacagtaaa atgtgcctga agagtatcgt
     1501 cggcgcgggg aatatttcta agtctcatat gtgtctcctt gtattttggt cgttatacta
     1561 gattgtatca taatatgcag catatgtcag gtcaaagatt tgcggatgtt tgagaatcgt
     1621 tcctgtttag gcagaggata acatggacgg atgttaaggg aatgttaaag ggagcgcatt
     1681 tttggaagat gtggcttgca ttttggagga aaaggtgtat catagggata tattattgct
     1741 gacaggaggc tttttatgga cgctattttg gataaattga cggaaattga aaatgcagcg
     1801 tctgcgattg ttcgtcatgc agaagagcaa aaagacgtac ttggacagga atttgatgaa
     1861 aaacggaaaa aatttgacga ggacttagaa aataaaacga aagcacgcct tgagaaaatc
     1921 cgggaacagt tggaaaaaga acagtcgaga gttcttaaca gcagcagcgg cgcaagtgag
     1981 gatacgatct gcagcctccg gaaagaatat gaagagaagc atacagaata tgcgcatgag
     2041 atattgagac ggatcacaga ggtgtaactt atgggaaatt tacttaagta cagcgggatt
     2101 gtgacaaaga tccgggcaat ggaggcgaag cttttgacgc cggagcagtt tgcggatata
     2161 gcaaatttac gaagtgttcc ggatattgcg gactatctgc ggaaaaatac ggcgtatgcg
     2221 gaagtgcttg acactttaac ggaagatcag atccatcgag gaaacataga gaaggtgctt
     2281 gtgcagtcgc tttatcacga ttacacaaag atttaccgtt tctgcgggca gagtcagaga
     2341 gaatttatga agctgatcat gaaaggatac gagatcgagc tgatcaatta ctgtctgcgt
     2401 atcgtgatca accattataa gaaaccgttt gatcttaatt ataaaagtgc gttttttgac
     2461 cggtattcgc aaatatctat cggaaagctg atcacagcca gaacgacaga tgagcttgtg
     2521 gaaaatctaa aagaaacgga gtattacgct ccgttgaaaa aaataaaaga taccgataat
     2581 gtgactcttt atgattacaa tctggcgttg gatctttatt attatacatc tacatggaaa
     2641 gagcagaaga agattttgaa aaagtcggat cttgagctgt ttatgagaga tcgtgggtca
     2701 aagatcgatc ttttgaatat tcagtggatc tacagggcga aaaagtatta caatatgaag
     2761 cccgcggata tctatctgat gctgattccg atacactata aattgtcaac agaattagtg
     2821 aaagatatgg tggaggctcc gggagtcgag gagtttgaaa atgtcgtaat aagaacaact
     2881 tatgcccggc attataattt caaacaaaat ttgacaatgg agcagatgta tgcggactgt
     2941 ctgcatcatc tgtacacggc agaccggcga aagaatccgt attcggtctc cgcgatcaac
     3001 acctatctct tcttaaaaga agaagaattg aaaaagctga caaccgttat ggaatgcgtc
     3061 cggtacggac tgaatccggg agaaacggca gcatacattg gaggtagaac ccgatgatcg
     3121 taaagatgaa atttattaat atatcaggtc cgcgaaatga tatcgaccgt gttaccgatc
     3181 agtatctttc gcggtatgag attcagcttg agtctgcgct gtcggaactt aagacagtag
     3241 ataacttaag accttttgtg gagctgaacc cctacaggga agtgttaagt aaggcaaatg
     3301 aatttgtcgg atatcttccg aatgcagaaa cagtagagcc ggatacgaaa ctcggtcttg
     3361 acgatatgtt tgagcttgta cgcaaggcgg atgaagatta tcgtacactt caggaaaaaa
     3421 aggaaaagct taagcagaaa attgaagaat acagggcgaa acagcagatc gtagcgccgt
     3481 ttcgaccgtt ggaatgcgat cttcaccgtg tgcttcatta tcgatatatc acttatcgtt
     3541 tcggccgtat cgcagtggaa ttttatcata agatgcaaaa atatcttatg gatgatctaa
     3601 aagcaatctt cgtagaagga gagcgggatg aaagttatgt ttatggagta tatttcgttt
     3661 cgcaggcgga gacacagaaa gttgacgcgg tattccggtc gctgcatttt gaaagaatcg
     3721 agttgaagga tgaatttgaa ggaactcccg atacggcata ccgggtactc gaaaacgaga
     3781 ttgcccgtgt caatttagaa attgaagatc tggataaaca ggcaggagag atgctggcgg
     3841 accgcgctcc gcagttagtt gcggcaaggg acaggctgga agaactgtcc aataacttcg
     3901 atgtgagaaa acttgcggcc agaatggaag ataaacagga agattactat attctgtgcg
     3961 gatggatggc ggaagatgat gtagataaat tcgttgagga agtaaaggaa gacgataaag
     4021 tattcgtcgt tgtggaagac gatcatgacg catatttcgg agaaccgccg acaaagctgg
     4081 aaaatccgaa attattcaaa ccatttgaaa tgttcgtcag tatgtatgga ctcccggcac
     4141 ataacgagat ggatccgacg atgtttgtcg gtctgacgta ctcatttatc tttggagtga
     4201 tgttcggaga tgtcgggcag ggactgcttt tgatgatcgg cggaggattg gtctataagt
     4261 ttaagaaagc gccgcttgcg gggattattg cgacggcagg tgttttttcc acgatcttcg
     4321 gatttctgtt tggaagtatt ttcggatttg aagacgttct tccggcgctg tggatacggc
     4381 cgatcgatca tatgacgact ttgccgtttc tcggaaagct gaatacggtg tttattgttg
     4441 cggtggcgtt cggtatgttt ctgatcatgg ttgcgatggt gcttcacatt atcaatgcgg
     4501 tacgagggaa aaatatggaa agcgcatggt ttgatccgaa cggtgcggca ggacttgtat
     4561 tttatcttgc ggtcgtagtg acagtcgttc tctttatgac gggaaatccg cttccgggcg
     4621 gaatcgttat ggcgatcatg ttcggaatcc cgcttttgct catctttttc agagaacccc
     4681 ttacgaggat gcttcagaag cgggcggata agatggagac aggaaaggca atgtttgttg
     4741 tgcagggatt ttttgaactc tttgaaacat tgctcagtta cttctctaat acgatttcct
     4801 ttatccgtat cggagcgttt gcggtgagtc atgccgcgat catggaagtt gtgctgcagt
     4861 tggcgggagc agagagcgga agtccgaact gggcaggcgt gatcttcgga aacctgttcg
     4921 tttgcggatt cgaaggtctg atcgtcggaa tccaggtgct gcgtctggaa tattacgaac
     4981 tgttcagccg tttttataaa ggcagcggtc atgcatttga tccgtatgca aaaagaaaga
     5041 caaagaaaaa cagccgaaaa tagatttctt aaaattcatc atatcatagg aggaacttac
     5101 aatgacaatg ggaacaaaaa tcgtttttat catcacgctg atcgtgagca ttatcgtacc
     5161 gtttgggtat tatcttcttg gggaaaagag cagaaaacgt tataaaagag caataggaac
     5221 gaatgctttc ttctttttcg gagcgtttgc gattgcatcg atcatgcttc ttggcggacc
     5281 gaatgtacag gctgcggaag ctgtgactgc cgatgcggga ttcgcaacag gcatgggata
     5341 tcttgcggct gcgatcgtta cgggactttc ctgtatcggc ggcggaattg cggttgccag
     5401 cgcagccagc gcagcgttag gtgcgatcag tgaagattca agcgtactcg gaaaatctct
     5461 tatttttgta ggtcttgcag aaggagtgtg tctttatggt ttgatcattt ccttcatgat
     5521 tttaggaaac ctgtaataaa gatacatgcg gacggcgcgg cgggagcagc gtaaacggta
     5581 aggcagaaag gaaaaggatc tgaagttatg aagatgtatc tgatcagcga caatgttgac
     5641 acatataccg ggatgcgtct ggcaggagta gacggcattg tcgttcatga acaagatgaa
     5701 ctaaagcagg cgttggaacg tgttcttgaa gacaaaagta taggaattgt acttttgaca
     5761 gagaaattcg gcagggagtt tccggcgatc atcgatacat tccgattgga acgtaagatg
     5821 ccgcttcttg tggaaattcc ggaccggcac ggaacgggac gaaaaaaaga ttttatcaca
     5881 tcttatataa cggaagcgat cggattgaaa ttataaggcg gagatattca ttccgacgaa
     5941 tgaagaatac agcggacaaa gcagtgatag agcagaaaat aaatcaggca aatgaagcag
     6001 ataaatcgta gaaaagggag ggctttcggt tgactcttga agaaaaactc acatatatgc
     6061 agactgtgtc tatggagcag gcgcgcgcag aaggaaatgc gatcatagat gcgcacagag
     6121 aagcgctgga aaaaatattc agcgaccata agtcagagat gatgcggcag gcggaaacgc
     6181 gcataaaagc agagacgaca aatgcaaaac ggacagtaag tcaggcattg gcaaagtctc
     6241 agctggagat taaacgaagc cagggaaaag tgcagcagga gctgaaagac aaagtgtttg
     6301 aagaagcgat gcagcttgtg aacgattata tgaaaacaaa agcatacgac gactttcttg
     6361 ttaagtgtat ccgaaaggca gagcggtttg caggcgatga cccggtcgtt atttatatta
     6421 atccgtccga tgaaaaaaaa cgtacggagc tggaaaaaga aaccggggcg aagcttacgg
     6481 tgagtgcgga agattttatc ggcggggtgc gcgcggtaat ccgttcgcgt aatattttga
     6541 tggaccattc gtttaaaacg cagatccagg acgaatatga caaatttttg tttttaggag
     6601 gtgacggaat tgcttaacac aggaacgatt tatggaatta acggacctgt catctacctg
     6661 aaaggaaata caggatttcg tatgtcggaa atggtttatg tcggaaaaca aaaattagta
     6721 ggcgaagtga tcgctcttga taaagacagg acgacgatcc aggtgtatga agaaacgaca
     6781 ggacttagac cgggagagga agttgtcgca agcggaaatg cggtttcggt aacgcttgcg
     6841 ccgggaatcc tgaataatat atttgacgga atcgagcgtc cgcttgagag aattgcagag
     6901 acaggcggcg cgtttattac aagaggagtc agcgtggatt cgcttgacag agagaaaaaa
     6961 tggaagactc acatcgtcgt tgaaaaaggt cagtaccttc atggcggaga tattatagcg
     7021 gaagtgccgg agacacatgc gatccttcac aaatgtatga ttcctccgga tgtggaagga
     7081 acggtaaaag aggttgtaaa agacggagag tatacgatcg atgaaactct cgttatatta
     7141 gaacttccgg atggcggtga aaagaagctg actatgacac agcggtggcc gatccgtgtg
     7201 ccccgcccga cacatcaccg atttccggcg tctgttccgc ttgtgaccgg acagcgtatc
     7261 atcgatacga tgtttccgat cgcaaaggga ggaactgctg cgattccggg aggattcgga
     7321 accggaaaga caatgactca gcatcagatc gccaaatggg cggatgcgga tattattatt
     7381 tacatcggat gcggagagcg tggaaatgag atgacgcagg ttctggaaga gttcggtgaa
     7441 ttgacagacc cgcggacagg caatccgctt atggacagaa caacgctgat cgcaaatacg
     7501 tcaaacatgc cggtggctgc ccgtgaggca agtatttata caggacttac gctggcggaa
     7561 tattaccggg atatgggata cgatgtggcg atcatggcag attctacgtc cagatgggcc
     7621 gaggcactga gggaactttc gggacgtttg gaagagatgc cggcagaaga aggatttccg
     7681 gcttatctgg catcaagatt gtcggtgttt tacgagagag ccggaatgat gcacaatctg
     7741 aacggaacag acggatcggt tacgatcatc ggagcggtat cgcctcaggg cggtgatttt
     7801 tccgagccgg tcacacagaa tacgaaacgt tttgtgcgct gtttttgggg actggataaa
     7861 agtcttgcgt attcgagaca ttttccggca attcattggc tcacaagtta cagcgaatat
     7921 ctgaacgatt taagccattg gtatcaggat aatgtatccc cgcagtttgt tgattataga
     7981 aataggttaa tggcgcttct gaatcaggag agcagcctgt tggagatcgt aaaattgatc
     8041 ggaagcgacg ttctgccgga tgatcagaag cttgtacttg agattgcccg cgtgatccgt
     8101 ttgggatttt tacagcagaa cgcatttcat aaagacgata catgtgtctc aatggaaaaa
     8161 cagtttttga tgatggacac aattttatat ctttataaac aggcaaggac gcttgtgacg
     8221 atggggcatc ctatgtctgt gctgaaggcg gaaaatattt tcgaccgtgt aatctcgatc
     8281 aaatatgatg tgccgaacga cagattggaa atgtttgctc aatatcatcg tgatatcgac
     8341 gcgttttatc agaatgtttt ggaaaagaat gcataaggag aaccgaagat catgtcaatc
     8401 gaatatttag gattaagcag catcaacggc ccgctgatcg cgctggaggg cgtacaggat
     8461 gcatttttcg acgagatcgt ggaattcgtc gtagacggta cgcagagaaa gatcggccgt
     8521 atcgtagaaa tatatgaaga taaagccgtt attcaggtat ttgaaggctc tgaaaatatg
     8581 tccctgaaaa atacgcatac aaaactgaca ggccatccga tggagatcgc gctttcgccg
     8641 gatatacttg gcagaacgtt taacgggatc ggaaatccga tcgacggact tggaccgatt
     8701 gcttctacgt taagaagaga cgtgaatgga tctcctctca atcctgtgaa gagagagtat
     8761 cctcgaaatt atatcagaac aggaatttct gcgatcgacg gactgacgac gctgatccgc
     8821 ggacagaaac tgccgatctt ttcgggaaat ggtctgcccc atgaccaact ggcggcgcag
     8881 attgtaaaac aggcgtctct gggagaaaat tcagatgaaa agttcgcgat cgtatttgcg
     8941 gcaatgggtg taaagcatga tgtggcggat ttcttccgca atacgttcga ggagagcggg
     9001 gttgcggatc acgtagccat gtttttgaat ctggcgaacg atcctgttgt ggaacgtctt
     9061 atcacgccga aagtggcatt gacggcggcg gaatatctgg cgtttgaaca gaatatgcat
     9121 attcttgtaa tcttgactga tatgacatcc tttgcagaag cgatgcgtga agtttcttcg
     9181 tcaaaaggtg agattccgag ccgaaaaggt tatccgggat acttgtacag tgaacttgcc
     9241 acgatttacg aaagagcggg gatcgtacag ggagtgaacg gttctgtaac acagcttccg
     9301 attcttacga tgccaaatga cgatatcaca catccgattc ccgatctgac gggatatatt
     9361 acagaagggc agattgtact tgaccgtccc cttaacggac agtcgatcta tccgccgatc
     9421 aacattcttc cgtcattgtc ccgtcttatg aaagacggta tcggcgaggg atatacgaga
     9481 gaagatcatc aggatctggc aaatcagttg ttctccgcat atgcaaaagt cggagatgcc
     9541 cgaaatctgg cgtctgttat aggtgaggat gaattgtcgc ctattgataa gaagtatctt
     9601 gagttcggaa aagaatttga agaaaaatat atcggtcagg gaatgaatga aaaccgtaca
     9661 atggaggaga cgcttgatct cggatggaaa ctgctcggac gtttgccgag agaagagctt
     9721 gaccgcgttg atacgaagat tcttgataaa tattataaac cgtctgaaga agaataagtt
     9781 agggtgcaga gaaactgatc ctttcagagt aaaaataaaa gcggggaagg aggaggcata
     9841 tggatccgcg tacatttccg acaaaaggaa atcttatgct ggcgaaaaat tcgcttgcgt
     9901 tggcgaagca aggttatgat ctgatggata aaaaaagaaa tatactgatc agagaactga
     9961 tggatctgat cgatgaagca cggacgatcc aagaagagat cgacacgacg ttcacttatg
    10021 cgtatgaatg tctgcaaagg gcgaacatcg aaaacggaat cagcaatgtt gagatgcttg
    10081 catatacagt tccgatcgaa aattcgatca cgatccagac gcgcagtatt atgggaacgg
    10141 aaatccctca tgtcaaatat atgcctgatg ccggaaagcc aacgtattca tttagtacga
    10201 cccgtgaatc gatcgataag gcgagagaag cattccgcaa ggtaaaagat ctgacggtga
    10261 aattgtctat ggtagaaaat gcggcgtacc gccttgcggg aaatatcaaa aagacgcaga
    10321 agagggcaaa tgcattgcag aatatcacga ttccgatgta tacatcactt gtatatacga
    10381 tcacgaatgc tttggaagaa aaagagcgtg aagaatttac ccgtttgaaa gtaattaaac
    10441 gaatgaaaac aaagaaacaa ggcggctgag aaaccttact gatcttttgg tatgcccgtt
    10501 tgaatatatt ttacgcatgc cggaatgaaa ggtgcaggaa agaggaagga atcacaatga
    10561 gcaatgagga acaaaagtca ataacaccgg gcatttactt gtggctgatt gcagtgaata
    10621 tttatatcag catgtttgcg tttggcggag gttatgttgt tgttcctctt gtacgccgaa
    10681 gttttgttga aaagaaaaag atatttacag aagatgagct gatggagatg tccgctgtgg
    10741 cgcagtcaag tccgggggcg atcgcggtga atctggcggc gttgtcgggg tataaggcgg
    10801 cgggaacggc aggggcggtg attagctgtg cagcttctgt aattccgccg cttgtgattc
    10861 tcggaatcat ttcaggattt tatgatttat ttattgcaaa taaaattgtt gccgctgttt
    10921 taaaaggaat gcaatccgga gttgcggctt tgatcgtcag tgttgtagca gatatgtgca
    10981 ctgtgatatt gaaagaaaaa tcattttttc ttttttccct tattccgatc gcctttatag
    11041 caaattttat ttttgatgta aatgtggcgc tgattctggc agtatgctgc gggctttgta
    11101 tccttcgggt ttacgtaaga agcggaggtc acaaatggaa attcttttaa aattattttt
    11161 gacatttttg aagatcggcc ttgtcagtat cggaggcggg tatgcggtaa tcccttcgat
    11221 ccatgaagaa gttgtagtaa aagcgggatg gatcggagaa aaagtatttg cggatattat
    11281 tacgatatct cagatgacgc ccggtcctct ggcagtgaat acatctacgt ttgtcggctt
    11341 gcagatcgca ggtgcggcag gcgcggttgt ggcaacagtc ggatgcgttt tttgcggagt
    11401 tgcgatttca ttgactctgt atcgtttttt tcagaagcat aaagagtcag tctatatttt
    11461 tgagacgctt aacggattga aagcagcttc tctgggattg atcgtatcag ccgcggcaac
    11521 gatcatattg ctttcttttt tcggaacagg agatccggat ttaaaaacaa tgaaagaatc
    11581 gcttgatatc ggcgcggtag ctattttcgg aggagtgttg tttttgtcgc ggaagcggaa
    11641 gatcaacccg atcatgctga tgctcgtaag cggagcggca ggtataattt tatacatgta
    11701 actgtaaaag taaaaaagta aagaaggcgg tcttgaatag aaaagcggca aagggtctga
    11761 taaggaaaga tcagaaacag aggaaagagg tttatgagag aaaagaaaaa taagaatgat
    11821 aatgtaaatg acgtaaatgg caaaagagat aataagaaag cggccggaaa atttaaaaat
    11881 tggacaacag ctcttagcat ggagctaagg gaacataaaa gttcggctat agtgtatttt
    11941 acattgaggg cgcttgtgat cattatgctc gtgctccaac ttttgaatcg gaattatgaa
    12001 aatgtatttt tgtgtatact tacgcttctt ttgcttgtga ttccgagttt ggcacagatt
    12061 acatttaaag tggaactgcc gacagcattg gagattatca ttcttgtatt tattttttcg
    12121 gcagagatac tcggcgagat cagtgaattt tatcttgtat ttccgttctg ggatacagtg
    12181 ttacatacat tgaacggttt tcttgcggcg gcgatcggat tttcgcttgt tgatctgctg
    12241 aacagaagtg accggacggt tttttcactg tcgccgcttt ttacggcaat cgtagcgttt
    12301 tgtttttcga tgacgatcgg agttgtgtgg gagttttttg agtttgggat ggatatgatc
    12361 atggaactcg atatgcagaa agatacggtt atccatacga ttcgatctgt tatgcttgat
    12421 ccgggcggac ataatgtgcc gtatgcgata caaaacataa cagatgttgc ggtgaacgga
    12481 caatctcttg gattgggagg atatcttgat atcggactgc ttgatacgat gcaggatttg
    12541 atcgttaatt ttatcggcgc ggctgtattt tccgtacttg gatttttcta tgtgaaaagc
    12601 agaggaaaag gaaaggtggc aggaagcttt ataccgagaa gaaaagcgca tgacagagac
    12661 tttttgagaa ttgcgagaga gtcttcggaa gtggagcaag atatacagga gaaatcgcag
    12721 aaagtgccga aaggcagtgc aaaaaacgga gacagatgac agaaagaaga aaaggtatat
    12781 gaaacaggcg tcataagcgg cgcttgggag acggcagtcg gtacaataga tgtggtttta
    12841 taaacattat cacagaaaca agtctatttt tgacaatgtt tgttaaaaaa tagacataaa
    12901 gattgtggaa agcgacaaaa tatattgcaa aaacagcggg tgcattgtat ataattttgt
    12961 cgctttccac aatctttatg tctatttttt aacaaa
//
LOCUS       DBMDXF010000036        12988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1397_length_12988_cov_2.171879, whole genome shotgun sequence.
ACCESSION   DBMDXF010000036 DBMDXF010000000
VERSION     DBMDXF010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_12988_cov_2.171879"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="ACZPD4_02655"
     CDS             <1..1048
                     /locus_tag="ACZPD4_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1378012.1"
                     /translation="HISLISLGNGTASRESEQFIVELLKEIPEQVQYVIVNEAGASVY
                     SASKLASEEFPKFDVGQRSAASIARRLQDPLAELVKIDPKSIGVGQYQHDMNQKKLGE
                     KLGGVVENCVNKVGVDLNTASAPLLSYISGISSAISKNIVAYREEHGKFTSRRELLKV
                     PKLGPKAFEQCAGFMRIRDGENPLDGTGVHPESYEAAKGLLQKQGYSPEDVAAHNLAG
                     LSLTIKDYKATAEELGIGEITLRDIVKELEKPARDPRDEMPKPILRTDVLEMKDLKEG
                     MILKGTVRNVIDFGVFVDIGVHQDGLVHISQITDRYIKHPLEAVSVGDVVDVKVMSVD
                     MKKKRIQLTMRGIS"
     gene            1104..1733
                     /locus_tag="ACZPD4_02660"
     CDS             1104..1733
                     /locus_tag="ACZPD4_02660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1378013.1"
                     /translation="MAKYRHIVFDVDGTLIDTRDCILYSLQEMLMTVKGERYEIEDLK
                     FVLANTSVKNMHLLGVEEEKIPGAVEMWVANEEKNIDMIRIFDGIEELLEKLEKKGST
                     LGVVTSRTREELELVLDQLPIRKYFSICICADDTKEHKPSAQPLLKYMELSGARKEET
                     VYIGDSASDMACALNAGVDCVHAVWGEPETEADATYRAGKPEQLEEIFL"
     gene            1885..2325
                     /gene="rpiB"
                     /locus_tag="ACZPD4_02665"
     CDS             1885..2325
                     /gene="rpiB"
                     /locus_tag="ACZPD4_02665"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1378014.1"
                     /translation="MKIGIGNDHSALELKAEIISFLKEKGHEVVDYGTNSSESCDYPV
                     YGEIVARAVAAGEVEQGILICGTGLGISLAANKVKGIRAAVCSEPFTAKMARVHNNCN
                     ILAFGARVVGAELAKMIVETWLDAEFEGGRHQRRVDLITAIEEK"
     gene            2328..3503
                     /locus_tag="ACZPD4_02670"
     CDS             2328..3503
                     /locus_tag="ACZPD4_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1378015.1"
                     /translation="MQSYHLTQWVLFFFIYSFIGWVWECCFVSVRKRRWVNRGFMYGP
                     MLPIYGFGALAVLISTIRVRDSIPLIFLFGMVGATLLEYVTGAVMERLFNVKYWDYSN
                     QKFNLNGYICLTSSLGWGLFSVLLVKFVHVPIEGAVLKIPTIIAEGIAFVLTVAAAVD
                     VTQSFNDAMDLKRILAQLEESKKQIRKIQEKLKVASEEFVEDYRQRAGEFVEDYRQRA
                     GEFVEDYRRRVEEHVEDYRGRAEEMAEDYKKLAEEHMDSYRQRAEEMVEGYKEEIKVQ
                     KKKRREQINSRKEEYLARVHARREERQRLLARLSERAELLMKEEFTSKVDELIGEDKR
                     EEYAKIKKNISREMTKMHDRTDRSYIRAARHLKGNPTAVSKRFSEALAELRKHMDEK"
     gene            3523..4161
                     /locus_tag="ACZPD4_02675"
     CDS             3523..4161
                     /locus_tag="ACZPD4_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR1378016.1"
                     /translation="MTKKQRALQVIERLKKEYPSAGCTLDYDQAWKLLVSVRLAAQCT
                     DARVNVVVEGLYEKYPTVEALAEADVDEIEKIVRPCGLGRSKARDISGCMKMIRDEYE
                     GKVPDDFDALMKLPGVGRKSANLIMGDVFGKPAIVTDTHCIRLVNRIGLVDGIKEPKK
                     VEMALWKIIPPQEGSDFCHRLVFHGRDVCTARTKPYCEKCCLADICKKNGVS"
     gene            4185..6719
                     /locus_tag="ACZPD4_02680"
     CDS             4185..6719
                     /locus_tag="ACZPD4_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1378017.1"
                     /translation="MKSLKLRDDFYWTGIIDDQLRVFDIIMYTEFGTTYNSYVMKAGD
                     KTVLFETAKAKFFDEYLEHLQEVIDIRKIDYLVVSHTEPDHAGSVERLLDYSPQMKVI
                     ATGCALGFLKEIVNRDFVGIPARDKEKMTIGNKTLEFLFVPNLHWPDTMYTFIEEEQI
                     LVTCDSFGAHYCLPEVVSSEIKNEADYQSALKYYYDCIIGPFKPFMLKALDRVETMDI
                     SMICTGHGPVLVGDRIRQVMKQYREWSTVVNPNEKKTVIIPYVSAYGYTKMLAEKIAE
                     GVKDSGDIDVRSYDMVEADAEKVNEELLFADGILLGTPTIVGEALKPIWDLTLAMFPA
                     THGGKYAGAFGSYGWSGEGVPNITQRLKQLKMKVSEGFRVRFKPSEADLVSAYEYGYQ
                     FGCIVQNKEPVKPKKKGARTLVKCLVCGEIFDSSLEICPVCGVGKENFIEVEAEESAV
                     LNNTEEYYVILGNGTAGFNAAKAIRERDKTGAVTIISDEEYPAYNRPMLTKSIVAGLS
                     ADQIAIVDPSWYEENKVFQMLGKKVASVDVNEKEVILESGEKVHFTRLIYALGSECFI
                     PPIEGSSLPEVVAIRRLADVEKLEKMMEHAAKAVVIGGGVLGLEAAWELKKAGIDVQV
                     LEAAPILMGRQLDENASDILRMFAEKSGVKISTGVSVEAVEGDGHVSGVRLSDGQVIP
                     AEVVVVSAGVRANTALAKEIGLDADRAVKVNERMETSVPNVYACGDCAEFNGANYAVW
                     PEASEQGRIAGANAAGDQIEYAATAPTLTFHGMNTALFAAGDNGKNPNLLYKTAEFKD
                     MGKGRYSKYYFLNNRLAGVIMIGDLSDMVKITEALEKHALYKDVLG"
     gene            6871..8169
                     /locus_tag="ACZPD4_02685"
     CDS             6871..8169
                     /locus_tag="ACZPD4_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1378018.1"
                     /translation="MVNTIYELAWIFFIYSFIGWCGEVIVAAVNRHKFVNRGFIAGPL
                     CPIYGTGAVAVAVFLPELKENLIFLFIGGMIVTSFVEYITGRLMEKILHKKWWDYSDQ
                     KFHLDGYICLRVSALWGVCSVLMIYFLNPFFCGLVNMIPRLIGEVILWVLLGLLIADG
                     LGSGIAVLELKRKKGRIEQITEELQKTSKFLENALTKRIQKRLVKAFPNIETEKDAEQ
                     EEKFAEGCGFFKLASLFIIGAFIGDIVETIFCLITTGRLMSRSSLVFGQFSIVWGIAC
                     ALLTWILYRYRDKSNWFIFLFGTILGGAYEYICSVFTEIAFGTVFWDYSKIPFNLGGR
                     INLLYCFFWGFAAVIWMKAVYPFLSRQIERLPKKAGRIICSLLLVVLTADMALSFAAL
                     ARYGERQEGKEGNGIIAEKLDEYFPDQFIEKRYENLKIAK"
     gene            8680..8750
                     /locus_tag="ACZPD4_02690"
     tRNA            8680..8750
                     /locus_tag="ACZPD4_02690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8712..8714,aa:Gly,seq:tcc)
     gene            8987..9649
                     /locus_tag="ACZPD4_02695"
     CDS             8987..9649
                     /locus_tag="ACZPD4_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1378019.1"
                     /translation="MRGDVTDSGIMRVLSKICDIVCLNVLWLVCSLPIITIGASTTAL
                     YTVMLKLVKNEEGYIFKGFLHAFKLNFKQSTIIWLILLLCGGVCFVDYRISEMMPGTL
                     GMVFKSVFLLLAFFLISVTIYSFALTARYENTIGATLKNALLLTVAKLPYTILMTALT
                     VGAVVLSWWNTGILMLTIPVWILAGTGILAFINSCILRRVFRVFDEKAEGGDEERTDM
                     EE"
     gene            9696..10343
                     /locus_tag="ACZPD4_02700"
     CDS             9696..10343
                     /locus_tag="ACZPD4_02700"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1378020.1"
                     /translation="MNFLEERIVKDGVVKEGNVLKVDSFLNHQMDIKLLDQMGAEFKR
                     RFAKKPINKILTIEASGIGIACVAAQHFDVPVVFAKKSQSINLDGEMYVAEVESFTHK
                     CRNHVIVSQKFLTPEDHVLIIDDFLANGCALQGLIQIVQSAGATVEGIGIAIEKGFQS
                     GGRIIRNLGFQLESLAIVDGMDAAAGTVTFRSQEEQKEAEQPAEKCEQCRSATLD"
     gene            10336..11004
                     /locus_tag="ACZPD4_02705"
     CDS             10336..11004
                     /locus_tag="ACZPD4_02705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1378021.1"
                     /translation="MIKKKPVSVKGLVFDMDGLLLDSEKVVKRSWDYAGKELGYENFG
                     DHIYNTVGFNLKRRTEYFKTNVDLDFPMDRFAQMTREYYYKIADKEGIAVKKGAPELL
                     NAAKSHGCMIGLATSSRQIHAEQSLKRAGLYDYFDGKVFGDTVKEGKPSPEIYLKACK
                     SIGIEPEDAVALEDAPSGAISAHAAGMRVIVVPDLVQPPEEVKQFLWKQAEDLERVIE
                     MIWG"
     gene            11206..12975
                     /locus_tag="ACZPD4_02710"
     CDS             11206..12975
                     /locus_tag="ACZPD4_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR1378022.1"
                     /translation="MKKEVRGGVSLVLASAMLVASPMAAGMTETVQAEELTVDSKVAQ
                     IQGESIIPRADQVPGNEELFAQYVEQTFYGEESESYRTPAVYGNIGQNVLTGADKYVY
                     NELKKIAANIADGKAADGSEPSGQYVSTKFTLSVAGQGITYEGGAFKGFSLSKVIAAL
                     LADCPYDLYWYNKTASTWFNGRISGREVVEIDINFPAADAYAGPEIEQQYKTKCTVDS
                     KKTGAASSAAENARKIIEKHKAEKDYEKMESYKEEICDLTSYNYDAVKPGVAYGDPWQ
                     MIYVFDGDESTNVVCEGYAKAFQYLCDMSDFLDPGYNCCSVTGMMRGGTGEGPHMWNI
                     VTIGDGNYLVDVTNSDDGTAGERGGLFMAWDKNNGSVENGYVFNTVYYDDAITFAYDG
                     RTKDLYGTGDNSVLKIASAEYEEPKGTAPVIEGVENGGNYYATQKITVRDADNDLASV
                     TVNGKQEAGTEISLSADNQNNRKKEYTIIAEDRRGNSTSCKITINPCSDLQKRISHLS
                     VDTVKVTDRALVQNTLKDAVTAVENAAEEEKTILAEVKTKCETLLAKIDEMTQPQDYI
                     RGDVDANSKVDVGDVRQIVVRHA"
BASE COUNT         3923 a         2196 c         3598 g         3271 t
ORIGIN      
        1 tcatatttca cttatttcgc ttggaaacgg aactgcttcc cgtgaatcag aacagtttat
       61 cgtggaactt ttgaaagaga tacctgagca ggttcagtat gtgatcgtca atgaagcggg
      121 agcttctgtc tactcggcaa gtaagcttgc aagtgaggaa tttccgaagt ttgatgtagg
      181 tcaaagaagc gcggcgtcca tcgcgagaag acttcaagat ccattggccg aacttgtgaa
      241 gatcgatccg aagtcgatag gcgtcggaca atatcagcat gatatgaatc agaagaaact
      301 gggagaaaag cttgggggag tcgtagagaa ctgcgttaat aaagtcggcg ttgatttgaa
      361 tacggcgtct gcgccgcttt tatcgtacat ttcgggaatc agttcggcga tttctaaaaa
      421 tattgtagcc tacagagagg agcacggaaa gtttacgagc agaagagaac ttctgaaagt
      481 gccgaaactt gggccgaaag cgtttgaaca atgtgcggga tttatgagaa tccgcgacgg
      541 agagaatccg cttgacggta cgggcgtcca tcctgaatca tatgaagcgg caaaaggcct
      601 tttgcaaaag caggggtatt caccggaaga tgtggcggct cataatctgg cgggattatc
      661 tcttacgata aaagactata aagcgacggc agaagaatta ggaatcggcg agatcaccct
      721 gcgcgatatc gtaaaagagt tggaaaagcc tgcgcgcgac ccgcgtgacg agatgccaaa
      781 accgattctt agaacggacg tactggaaat gaaagattta aaagaaggaa tgatattgaa
      841 aggaacggtg cgaaacgtca ttgatttcgg cgtgtttgtg gatatcggag ttcatcagga
      901 cggtctcgta catatttccc agattacaga ccgttatatc aaacatcccc ttgaggcagt
      961 gagcgtcgga gacgttgtgg atgtaaaggt gatgagcgtg gatatgaaaa agaagaggat
     1021 acagcttacg atgagaggaa tttcctgaaa aacgaaaaaa gaaagaagaa aaaagatttg
     1081 agaagacagc gggaggtata ggaatggcaa aatacagaca tattgtcttt gacgtggacg
     1141 gaacactgat cgatacgaga gactgcatat tatattcatt gcaggagatg ctcatgacgg
     1201 taaaaggaga gcggtacgag atagaagatc tgaagtttgt gctggctaat acatctgtga
     1261 aaaatatgca tcttctcggg gtggaagaag aaaaaatccc gggcgcagtc gaaatgtggg
     1321 tggcaaatga agagaagaac atcgatatga tccgcatttt tgacggaata gaagaacttc
     1381 tggaaaaact ggagaaaaaa ggaagtacgc ttggtgtcgt aacatcaaga acacgcgagg
     1441 aactggagct tgttcttgat cagcttccga tacgaaaata tttttcgata tgcatatgtg
     1501 ccgatgacac gaaagagcat aaaccgtcgg cacagccgct gctgaaatat atggaactgt
     1561 caggagcgcg gaaagaagaa accgtctata tcggagacag cgcttcagat atggcatgcg
     1621 ccctcaatgc gggagtggat tgtgttcatg cggtatgggg cgaaccggaa acagaagcgg
     1681 atgcgactta tcgcgccggg aaaccggaac aacttgagga aatattttta taaaaattgt
     1741 ttgacagaac cggctttcta aagagcgttt tgtaaattag gtttattgaa tcacagcgcc
     1801 ggatttgtta tgatgataga gaaatcaatt ttattgtaaa gaagcatgta aataaaataa
     1861 agtggaaaag aaaaggagaa gaatatgaag atcggaatcg gaaatgacca ttcagcatta
     1921 gaattaaagg cggaaatcat tagtttcctg aaagaaaaag gacatgaagt agtagattac
     1981 ggaacaaatt caagcgagag ctgtgattat ccggtctacg gggaaatcgt ggcaagagca
     2041 gtggcagcag gcgaggttga acagggaatc ctgatctgcg gaacaggtct tggaatttct
     2101 cttgcggcaa ataaagtaaa aggaatccgc gccgcggttt gcagcgagcc gtttacggca
     2161 aagatggcaa gagtgcacaa taactgtaat atcctggcgt tcggagctcg tgtggtagga
     2221 gcggagcttg cgaaaatgat cgtagagaca tggcttgacg ctgagtttga aggaggacgt
     2281 catcaaagaa gagttgacct gattacagcg attgaggaga aataaacatg cagtcatatc
     2341 atctgacaca atgggtattg ttctttttca tttacagttt tatcggctgg gtatgggaat
     2401 gctgctttgt ttctgtgcgg aaacgccgct gggtaaaccg cggatttatg tacgggccga
     2461 tgctgccgat ctatggattc ggagcgcttg cggtcttgat cagtacgatc agagtcagag
     2521 acagtattcc gttgatcttt ttattcggca tggttggagc aacgctgttg gaatacgtta
     2581 caggggcggt tatggagaga cttttcaatg tgaaatactg ggattacagc aatcagaaat
     2641 ttaatctgaa cggatatatt tgtcttacct cgtctttggg gtggggattg ttttccgttt
     2701 tacttgttaa gtttgttcat gtaccgatcg agggagcggt gctgaagatt ccgacgatta
     2761 ttgcggaggg tatcgcattt gtgctgactg ttgcggcggc ggtcgatgtg acgcagtcgt
     2821 ttaatgatgc gatggatctg aagcggattc ttgcacagct tgaagaaagc aaaaaacaga
     2881 tcaggaagat acaggagaaa ttaaaagtcg cttctgaaga atttgtggaa gattatcgac
     2941 agcgcgcagg tgagtttgtg gaagattatc gacagcgcgc aggtgagttt gtagaagatt
     3001 atcgcaggcg tgtagaagaa catgtagagg attaccgcgg acgtgcggaa gaaatggcgg
     3061 aagattacaa aaaactggca gaagagcata tggacagtta ccgtcagcgc gcagaagaaa
     3121 tggtagaggg atacaaagaa gagatcaaag ttcaaaagaa gaaacgccgc gaacaaatca
     3181 attcacgcaa agaagaatat ctggcgcgtg ttcatgcaag gcgcgaagag agacagcgtc
     3241 ttcttgcaag attgtcggag agggcggaac tgttgatgaa ggaagaattt acatcaaagg
     3301 ttgacgaatt gatcggcgaa gataaacggg aagaatatgc gaagatcaag aaaaatattt
     3361 cccgggagat gacgaagatg cacgacagaa cagacagaag ctatatccgt gcagcaagac
     3421 atttgaaagg caatccgacg gctgtgtcaa aaagattttc agaagctctt gccgaactga
     3481 gaaaacatat ggatgagaag tgatagcgga gggataccaa agatgacaaa aaagcagcgt
     3541 gcgttacagg tgatcgagcg gctgaaaaaa gaatatccgt ccgccggctg tacgcttgat
     3601 tatgatcagg catggaaact gcttgtcagt gtgcggcttg cggcacagtg tacagatgcc
     3661 cgtgtcaatg tagtggtgga agggttatat gaaaaatatc cgacagtgga agctttggcg
     3721 gaagcggatg tagatgagat tgagaaaatt gtgcgtccgt gcggtcttgg cagaagcaag
     3781 gcgcgcgata tcagcggctg tatgaagatg atccgggatg agtatgaagg gaaagtgccg
     3841 gatgattttg acgcgctgat gaaacttccg ggagtcggaa gaaaaagcgc aaaccttatc
     3901 atgggtgatg tgttcgggaa acctgcgatt gtgacagata cgcattgtat ccggcttgta
     3961 aaccggatcg gactcgtaga tgggataaaa gaaccgaaaa aggtcgagat ggcattgtgg
     4021 aagatcattc ctcctcagga gggaagcgat ttctgtcaca ggcttgtctt ccacgggcgg
     4081 gatgtgtgta cggcaaggac aaagccgtac tgcgagaagt gctgtctggc ggatatttgc
     4141 aagaaaaacg gagtttcata aaaaagaaaa agaggaggca gaaaatgaaa agtttgaaat
     4201 taagagatga tttttattgg acaggtatta ttgacgatca actcagagta tttgatatta
     4261 tcatgtatac ggaattcggt acaacttata attcttatgt gatgaaggcg ggggacaaaa
     4321 cagttctttt cgagactgca aaagcgaagt tttttgacga atatttggaa catcttcaag
     4381 aagtgatcga cattcggaaa atcgattatc ttgttgtaag tcatacagag ccggatcatg
     4441 ccggaagcgt agaacgcctt ctcgattata gtccgcagat gaaggtgatc gctacgggat
     4501 gtgcgctcgg tttcctgaaa gagatcgtaa accgggattt tgtcggcatt ccggcaaggg
     4561 ataaagagaa gatgacgatc ggaaataaga cgcttgagtt tttgtttgtt ccgaatcttc
     4621 attggccgga tacgatgtac acttttattg aggaagaaca gattcttgta acatgtgatt
     4681 ctttcggtgc acattactgt ctcccggaag tcgtatcaag tgagattaaa aatgaggcgg
     4741 attatcaaag tgctttgaaa tattattatg actgcattat cggaccgttc aaacctttta
     4801 tgcttaaagc tctcgaccgt gttgaaacaa tggatatttc catgatctgt acggggcatg
     4861 gaccggttct cgtaggagat cggatcagac aggttatgaa gcaatacaga gaatggtcga
     4921 cagtggtcaa tccgaatgag aaaaaaacag ttattatccc gtatgtgagc gcatatggct
     4981 acacgaagat gcttgcggaa aaaatcgcgg aaggagtcaa agacagcggc gatatcgacg
     5041 tcaggtctta tgacatggtt gaggcagacg cagagaaagt gaacgaagaa cttttgtttg
     5101 cggacggaat attgcttgga actcctacga ttgtcggaga agcgctcaag ccgatatggg
     5161 atcttacact tgcaatgttt ccggctactc acggaggaaa atatgccggc gcattcggaa
     5221 gctacggttg gagcggagaa ggagtgccga atattacgca gcgtctgaaa caactgaaaa
     5281 tgaaagtatc ggaaggattc cgcgttcggt ttaaaccatc ggaagcggat cttgtgagcg
     5341 cttatgagta cggatatcag ttcgggtgca tcgtgcagaa caaagaacct gtaaagccga
     5401 agaaaaaagg cgccagaacg cttgtgaaat gtcttgtctg cggagaaatt ttcgattctt
     5461 cgctcgagat atgtccggtg tgcggagtcg gaaaagagaa ttttatagaa gttgaagcag
     5521 aagaaagcgc cgttttgaat aatacagaag aatattatgt tatcctcgga aacggaacgg
     5581 caggatttaa cgctgccaag gcgataaggg aacgtgataa aacgggagct gttacgatca
     5641 tttctgatga agagtatccg gcatacaacc gtccgatgct gacaaagtcg atcgtggcag
     5701 gattgagcgc ggatcagatc gcgatcgtag atccgtcatg gtatgaagaa aacaaagtgt
     5761 tccaaatgtt aggaaagaaa gtggcgtctg tcgatgtaaa tgaaaaagaa gtgattcttg
     5821 aaagcggaga gaaagtgcat ttcacgagac tgatctatgc acttggaagc gagtgcttta
     5881 tacctccgat cgaaggaagt tctttgccgg aagtagttgc gatccgccgc cttgcagatg
     5941 tggaaaaact ggaaaaaatg atggaacatg cggcgaaagc ggttgtgatc ggaggcggtg
     6001 tgcttggtct ggaagctgcc tgggaactga aaaaagcggg gattgatgta caagtgttag
     6061 aagctgctcc gattcttatg ggacgtcagc ttgacgagaa cgcttcggac attttgagga
     6121 tgttcgccga aaagagcggt gtgaagatca gtacgggcgt ttccgtggaa gcagtggaag
     6181 gagacggaca tgtatcggga gtccgcttgt ctgacggtca ggtgattcct gcggaagttg
     6241 tcgttgtatc ggcgggagtg cgtgcaaata cagcattggc taaggagatc ggacttgatg
     6301 cggaccgtgc cgttaaagtg aacgaacgga tggaaacgag tgttccgaac gtatatgcat
     6361 gcggtgactg tgcggaattt aacggggcaa actatgctgt ttggccggag gcttccgaac
     6421 agggacgtat cgcgggcgca aatgcggcag gggatcagat tgagtatgcg gcgacagctc
     6481 cgacattgac attccatggg atgaacacgg ctcttttcgc agccggagat aacgggaaaa
     6541 atccgaatct gctttataag acggcagaat ttaaagatat gggaaaagga agatacagta
     6601 aatattactt tttaaacaac cgccttgcag gcgttatcat gatcggagat ctgtccgata
     6661 tggtcaaaat cacggaggca ttggaaaaac atgccctgta taaagatgtg ctcggataga
     6721 aaagaacgca tcacataaag cggcgcctcc cgggcaggag tgagtgtctc gtataaaacc
     6781 gtgtccgggg ggattatttt tatcgaaggt gcatctgaaa ctgcgtcttt gcaaaaaaga
     6841 tgtgcgggaa aggaattcaa aagagaaaat atggtaaaca caatatatga actggcatgg
     6901 atctttttta tttattcatt tatcggatgg tgcggcgagg tcatcgttgc ggcggttaac
     6961 aggcacaagt ttgtcaacag gggatttatc gcgggaccgc tctgcccgat ctacggaacg
     7021 ggagcggtgg cggtggcggt gtttttgccg gaattaaaag aaaatctgat ctttcttttt
     7081 atcggcggaa tgatcgttac ttcgttcgta gaatatatta cgggaaggct gatggaaaag
     7141 atactgcata aaaagtggtg ggattattca gatcagaaat ttcatctgga cggttacatt
     7201 tgtctgcggg tatctgcttt atggggcgtg tgcagtgtac tgatgatcta cttcctgaat
     7261 ccgttttttt gcggtcttgt caatatgata ccccgtttga tcggagaagt gatcttatgg
     7321 gtgcttttag gcctgcttat tgcggatggt ctcggttcgg gaattgcggt tcttgaattg
     7381 aaaagaaaga aaggtcggat cgagcagatt acggaagagc tgcagaaaac gtctaaattt
     7441 cttgaaaacg ctctgacaaa acggatccag aaaagactgg taaaggcatt tccgaatatt
     7501 gagactgaaa aagatgctga acaggaagaa aaatttgcgg aaggatgcgg attttttaaa
     7561 ctggcgagtc tgtttatcat cggagcattt atcggggata tagtagagac gatattctgc
     7621 cttattacaa ccgggcgtct tatgagccgc agcagtcttg tgtttggtca gttcagtatc
     7681 gtgtggggaa ttgcatgtgc gcttctgaca tggattttat atagataccg tgataagagt
     7741 aattggttta tattcctttt cggaacaata ctcgggggcg catatgaata catatgcagt
     7801 gtttttacgg agattgcctt tggaacagtg ttctgggatt atagtaagat cccgtttaat
     7861 ctgggaggaa gaattaacct gttgtattgt ttcttctggg gatttgccgc cgttatctgg
     7921 atgaaagcgg tttacccgtt tttgtcaaga cagatcgaac gattgccgaa aaaggcggga
     7981 cgtatcatct gctccttgct gcttgttgtt ctgacggcgg atatggcgct gtcatttgcc
     8041 gctttggcgc gttacggaga acgtcaggaa ggaaaagaag gaaacgggat tatcgctgaa
     8101 aagttagatg agtatttccc ggatcagttt atcgagaaac ggtatgaaaa tctgaaaata
     8161 gcgaaataaa taaaacagat aaaacaaaaa accggaatgt gccttttggc gcttccggtt
     8221 tttgctgatg tgcggccctt cgccgggaaa ttatgatttg gaattcagag actgcagttc
     8281 gcgaagaaag tccagatcat cttgcctcaa ttttatgttt gtaacataat ttttatcgga
     8341 aggcgatgtg acgctgattt cgatcactgt gccttcttcc aaagcgttat gagacaccgc
     8401 gttcaaaaaa gaaggaaatt tcgggtgatt tttacgaaaa gtttccagat gtttttttaa
     8461 agcttgtaaa cgtgcgattt gtgataaatt caagtaacac aactcctttt tggttattcg
     8521 gaatattttc ctttatatga tttcgatttg tgaaaatcta tttaatctta taacaaagat
     8581 tcaataaaaa agcaaataaa aaaataaaat aaaaaaagta aaaatgttct tgcaattatc
     8641 tttatcatgt ggtagtatac tacttgtgtc agagatgacg cgggtgtagt tcaatggtag
     8701 aacaccagcc ttccaagctg gatacgtggg ttcgattccc atcacccgct ctattttttt
     8761 atcgtaacag ttcagccggt ttcagccgtt gtggttggac tgtttttttg taacggcgaa
     8821 gagccgaatc ccggacttgc ctgggaactt gacggctgct tgcgattccg gaacgcatct
     8881 tttatcgctt ccggtgatta tccgaaagaa gatgtgaaaa gcctgtgcta tacaggcttt
     8941 tctgtgctat actgtagttt acaatagaaa aaacgggagg cggatcatgc ggggtgatgt
     9001 tactgacagc ggtattatgc gtgtgctttc aaagatttgc gatatcgtgt gtctgaatgt
     9061 tttgtggctt gtttgctcac tgccgatcat tacgatcgga gcatcgacaa cagcgttgta
     9121 tacagtaatg ctgaaacttg taaaaaatga agaaggttat atatttaaag ggtttcttca
     9181 tgcatttaag ttgaatttta agcaaagtac aatcatatgg cttatattgc ttttgtgcgg
     9241 aggcgtttgt tttgttgatt acagaatatc ggaaatgatg ccggggacac tgggaatggt
     9301 gtttaaaagt gtttttttac tgcttgcatt ttttttgatt tcagtaacga tttattcatt
     9361 tgcgctgact gcaagatatg aaaatacgat aggggctacg ctgaaaaatg cgctcctttt
     9421 aacggtggca aaattacctt atacaatttt gatgacagcg ctgacagtcg gagcggttgt
     9481 tttgtcgtgg tggaatacgg gaatccttat gcttacgatc ccggtctgga tcttggcggg
     9541 aacaggtatt cttgctttta ttaattcctg tatattaaga agagtgttcc gtgtatttga
     9601 tgaaaaggcg gaaggcggcg acgaagaaag aaccgatatg gaagaataag atggaaaaat
     9661 aatctgcaca ggcaggaaaa cgagaggagc agaagatgaa ttttttagaa gaacgcattg
     9721 taaaagacgg ggttgtgaaa gaaggaaacg tattgaaagt agacagcttc ctgaatcatc
     9781 agatggacat taagctgttg gatcagatgg gggctgaatt taagagacgt tttgcgaaaa
     9841 agccgattaa taaaatttta acgatcgaag cgtcggggat cggtattgcc tgcgtggcgg
     9901 cacagcattt tgatgtgccg gttgtgtttg caaagaaatc gcagagtatc aatcttgacg
     9961 gagaaatgta tgtagcggaa gtggagtcat ttacacacaa atgcagaaat catgtgatcg
    10021 tatctcaaaa attcctgacg ccggaagatc atgttcttat tatcgacgat tttcttgcga
    10081 acggatgcgc tcttcaggga ttgatccaga tcgttcagtc cgcaggcgcg acagtagaag
    10141 gaatcgggat cgcgattgaa aaaggctttc agtcgggagg aaggatcatc cgcaacctcg
    10201 gttttcagct tgagtctctg gcaatcgttg acggaatgga tgctgcggcg ggaacggtga
    10261 cgtttcgcag ccaggaagag caaaaagagg cggagcagcc cgcagaaaaa tgtgaacagt
    10321 gcaggagtgc gactcttgat taagaaaaaa ccggtctctg tgaaggggct ggtttttgac
    10381 atggacggac ttcttcttga ctcggaaaaa gttgtaaaac ggtcttggga ttatgcggga
    10441 aaagaacttg gatatgaaaa cttcggagat cacatctata atacagtcgg atttaacttg
    10501 aagaggcgga cagaatattt caagacgaac gttgatttgg attttccgat ggatcgattt
    10561 gcgcaaatga cacgggaata ttattataag attgcggata aagaaggaat agcggtgaaa
    10621 aaaggcgctc cggaattgct gaatgccgca aaaagtcatg gatgtatgat cggacttgca
    10681 acgtcttcgc ggcagataca cgcagagcag tctttgaaaa gagcgggatt gtacgattat
    10741 tttgacggca aagtgttcgg ggatacagtg aaggaaggaa agccttcgcc ggagatttat
    10801 ctgaaagcgt gtaaaagtat cgggattgag ccggaagatg cggtcgcttt ggaagacgcg
    10861 ccttcaggag caatatcggc acatgcggca ggtatgcgcg tgatcgttgt tcccgatctt
    10921 gtgcagcctc cggaagaagt aaaacaattc ctatggaaac aagcggaaga tcttgagcgg
    10981 gtgatagaga tgatatgggg atgattcgtt atattgcaca taagccggag aaaagtttct
    11041 tgaaaaaatt atgaaaacaa aagaaatgtt taaataatgt acaaaaaata aaatgatgtg
    11101 tcgatcatct tttaagtgaa ctgaatttgt gataaaattg cataatcatg taaagtgttt
    11161 tacaggcaga cggaaaaaga tggaagaagt ttatggaggc ggaaaatgaa aaaggaagta
    11221 cgaggcgggg tcagtcttgt tcttgcatcg gcaatgcttg tggcatcgcc gatggcggca
    11281 ggaatgacgg aaacagttca ggcagaggaa cttacagtag atagtaaagt tgctcagatt
    11341 caaggagaga gcatcattcc gagagcggat caagttcccg gaaacgagga gttgtttgct
    11401 cagtatgtag aacagacatt ttacggtgaa gagagcgaaa gctatcgtac gcctgcagtt
    11461 tacggcaata tcgggcagaa tgttttaacc ggagcggaca aatatgtata taatgaactg
    11521 aaaaaaatag ccgctaatat cgcggacgga aaagcggcgg acggaagcga accgtcagga
    11581 cagtatgtat ctacgaaatt tacgctttcc gttgcggggc aggggattac atatgaaggc
    11641 ggcgcattta aaggatttag tttaagtaaa gtgattgcgg cgcttcttgc ggactgtccg
    11701 tatgatttgt actggtataa taaaacggcg tccacatggt ttaacggcag aatttcggga
    11761 cgggaagttg tggagattga tataaatttc ccggctgcgg atgcttatgc gggaccggaa
    11821 atcgaacagc aatacaagac gaagtgtacg gttgacagca agaagacagg ggcagccagt
    11881 tcggcggcgg agaatgcgcg aaaaatcata gagaagcata aagccgaaaa agattatgag
    11941 aagatggaat cttataaaga agaaatctgt gatctgacat cgtataatta tgatgctgtg
    12001 aaaccgggtg tcgcttacgg agatccgtgg cagatgatct atgtatttga cggagatgaa
    12061 agtacgaatg ttgtatgcga gggatatgca aaagcatttc agtatttatg cgatatgagc
    12121 gattttcttg atccgggata taattgctgc agcgtaaccg gcatgatgag aggcggtacg
    12181 ggagaaggac cgcatatgtg gaatatcgtg acgatcggag acggaaatta tcttgtggat
    12241 gtgacgaaca gcgatgacgg gacagcggga gagcgcggcg gactgttcat ggcatgggat
    12301 aagaacaacg gaagcgttga aaacggttat gtatttaata ctgtttatta tgatgatgct
    12361 attacttttg cctatgacgg acggacgaaa gatttgtatg gaacaggaga taactctgtt
    12421 ttgaagatcg cttcagcaga gtatgaagag ccgaagggga cagctccggt tattgaggga
    12481 gtggaaaacg gtggaaatta ttacgctaca caaaagataa cggtaagaga cgctgacaac
    12541 gatcttgcat ctgtgactgt aaacgggaaa caagaagcag gaacggaaat ttctctttcc
    12601 gcagataatc aaaataatcg gaagaaagag tacacgatca tagcagagga tcgcagagga
    12661 aacagtacaa gctgcaaaat aacgatcaat ccttgttccg accttcagaa aaggatctcg
    12721 catctgtctg tggacacagt aaaggttaca gacagagcgt tagtgcaaaa cacattgaaa
    12781 gacgcggtaa cagcagttga aaatgcagcg gaagaagaga agacaattct tgcagaagta
    12841 aaaacaaagt gtgaaacact tcttgcaaaa atcgatgaga tgactcagcc gcaggactat
    12901 atcagaggcg atgtggatgc caacagtaaa gtcgatgtcg gagacgtccg tcagatcgtt
    12961 gtacgtcacg catagattgt acatatct
//
LOCUS       DBMDXF010000037        12939 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1401_length_12939_cov_2.324038, whole genome shotgun sequence.
ACCESSION   DBMDXF010000037 DBMDXF010000000
VERSION     DBMDXF010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12939)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_12939_cov_2.324038"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            223..3450
                     /gene="carB"
                     /locus_tag="ACZPD4_02715"
     CDS             223..3450
                     /gene="carB"
                     /locus_tag="ACZPD4_02715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1378023.1"
                     /translation="MAKRNDIHKVLIIGSGPIIIGQACEFDYSGTQACKALRNLGYEI
                     VLVNSNPATIMTDPEIADVTYIEPLNVDRIEQIIKKERPDALLPNLGGQSGLNLCSEL
                     AQAGVLEKYNVEVIGVQVDAIERGEDRIEFKHTMDQLGIEMARSEVAYSVDEALAIAE
                     KLGYPVVLRPAYTMGGAGGGLVYNVDELKTVCARGLQASLVGQVLVEESILGWEELEL
                     EVVRDAEGNMITVCFIENIDPLGVHTGDSFCSAPMLTISEEVQQRLQEKSHKVVDSVK
                     VIGGCNVQWAHDPKTDRDIIIEINPRTSRSSALASKATGFPIALVSAMLATGMTLKDI
                     PCGKYGTLDKYVPDGDYVVIKFARWAFEKFKGVEDKLGTQMRAVGEVMSIGKNFKEAF
                     QKAIRSLETGRYGLGHAKDFDGKTKEELLKSLRYATSERYFVIYEALRKGASVEEIHE
                     LTKVKHYFLEQMKELVEEEEALLAFKGGLPEDNALIAAKKDGFSDKYLSQILEVDEDE
                     IRNKRISLGVEETWEGVHVSGTQDRAYYYSTYNGTDKNPISEEKPKIMILGGGPNRIG
                     QGIEFDYCCVHASLALKKLGFETIIVNCNPETVSTDYDTSDKLYFEPLTLEDVLSIYK
                     KEKPVGVIAQFGGQTPLNLAADLERNGVKILGTAPSVIDLAEDRDLFREMMDKLEIPM
                     PESGMAVTVDDALEIAEKIGYPVMVRPSYVLGGRGMEVVYDAESMVGYMKAAVGVTPD
                     RPILIDRFLGHATECEADAISDGTHAFVPAVMEHIELAGVHSGDSACIIPSKNISEEN
                     VKTIKEYTRKIAEEMHVKGLMNMQYAIENGKVYVLEANPRASRTVPLVSKVCNIRMVP
                     LAIDIVTSELTGRPSPVPALKEQNIPYYGVKEAVFPFNMFPEVDPLLGPEMRSTGEVL
                     GLSKSFGEAFYKAQEATQTKLPLEGTVLISVSNRDKPEVVEVAADFAKAGFKIIASDH
                     TCSLLKENGIEAEKINKLQEGRPNMLDVITNGKVDMIVNTPSGKESKNDDSYLRKAAI
                     KKKVPYMTTMAAAKAAANGILAVKKHGSKEVKSLQEIHGEITDKE"
     gene            3764..8935
                     /locus_tag="ACZPD4_02720"
     CDS             3764..8935
                     /locus_tag="ACZPD4_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1378024.1"
                     /translation="MKKRMFHKGMAAVLAVSLTTGGALPFMAQTVHAEDTAAEQGQIP
                     EKKSGVVTLEKTDGSYVFGNEYLKRTFSLSADKVLSTKEITNYRTGTTPTVFTPQAGS
                     EEFIINTLDNNSEGEDSGFVAPKKKLDTNGWTAEADSVATNEGANGGADKMFDGKNDT
                     YYHSKYNEGTDAERKYPHNIYVDFGAEKSFQSLRYQQRVDAQGNPTVSGHVKSYKIYT
                     GDSIDALKQATDAQPVAEGSFDNKKETYVNLKEKVTAKCVRIEFVDCHDPSDSNVSKD
                     VACCSEFDFFEDTATFPEATDDATQLKTSEMKVQGEPELTEKDGVKTLTFTFEPKRVR
                     GVDYTIKEVITMKDGDSFMRKRLDISVGEGQAEKAKIDYIDLENMQISQDDLKKDEYW
                     SIKDNMADNPDMGGMKGDYLELGQPYYVGAMYWGCEFPETENKIKGSNSFIRYYYGKS
                     LKSDDKFEYNEGNENGKMTTWDAVVGAARSRDYSVTQSDFYEYIETIAIDTEFRQQYN
                     SWYDNMKEITDEIIQKSFFEIEKGFTQYGIAPLDSYVVDDGWTNYSSFWDFNNKFPNE
                     LYNSSLQVNQLASNFGLWLGPRGGYGTERTIANWIASNGLGSVNNQSGGDINISDARY
                     LTKLNKDVFCEYQDKFDINYWKLDGMLLNPSTEQSEYYVTGNPLYTISETYERWTDIF
                     EDMRDNRAGKDLWLNMTSYTNPSPWHVQWVNSVWMQNTGDTGYTDSFNATDEEAMLTY
                     RDNAYYNFLNEREWQLPNKYFYNHDPVYGLTANDAYHRPDIKYTDDEMRNHLYMLGTR
                     GTAFWEYYYSYSMFDDNKWQINAEAAKWIEDNFDILQKSQMFGGKPNDGNVYGYSCWN
                     GKEGILSIRNPKNEAQSYKVTYDRLIGVGEDLGTVYGKVVVGDQRHQTDEPLTYGKEV
                     TYTLNPKEVLILQFGEKDETPAKILSVEGNGKEAEVEFDETIRTPEAGMFKVDGYEVT
                     KAELKADRRTVKLTLDKELKDARTVSVSVDGVKDTVGNTSKVSAQNDAFKDGIITGVI
                     SDDLKDGAVSTKAKYSVDGHGGFTVTGKIKTDSKDVVLAEQKGAYKVGIDGEGYLTFE
                     FNDMKITSKYDQKTVDKANDSYTSETKGIAADGKEHQFSAVKEINGMIKLYLDGKVVA
                     STYSEDKANPEIAKGETIFAQGLTKDEVSYITVLDRSLAYDEVKDLIDTEDNVVLAKN
                     NPKVKVTAYDATVNTAVAEKPDRPFSMVNDGVKSTANYLELTDTSDSQNHSRYVQFDL
                     GDEYDLMKIHMTRYWDGSRKYGPTVIQLSTDENFAADKTTTVYNSDKDNVHKQGAGKD
                     EFYVETAAGKEMWNAENSEPVTARYIRVYVNGRENNQGTSDHIVEFEAYGAKDGGAII
                     RPDRPEEPEKPVLTGVTASVEKAELKVGETTKATATIMPEGAEGVELAWTSSDDKIAT
                     VDKDGNVKAVAEGTATLTVTATQGSGDGAVTKTATVDVTVIKDGGTDPEPEKPVLTGV
                     KASVEKAELKVGETTKATATIMPEGAEGVELAWTSSDDKIATVDKDGNVKAVAEGKAT
                     LTVTATQGSGDSAVTKTATVDVTVIKDGGTDPEPEKPVVLTGVKASVKKADLKVGETT
                     KATAKITPEKAENVTFAWASSDKKVATVDADGNVKAVGKGTAKLTVTATQGSGADAVK
                     VTDTVKVTVTKDGGQTPQEDGKTPPKTGDETAPFFPFVLALAAGAVVMITRKKNG"
     gene            9238..9624
                     /locus_tag="ACZPD4_02725"
     CDS             9238..9624
                     /locus_tag="ACZPD4_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hippurate hydrolase"
                     /protein_id="HIR1378025.1"
                     /translation="MLSVVREYKDYSVLGHMDLIARYDEKGVYPFEKIKPIVEEILQV
                     VIADGKGLEVNTSSYRYGLSDTTPSVEILKRYRELGGKIVTIGSDSHKPEHLGAYIEE
                     TKEMLRKAGYTQFCTYERMSPVFHDL"
     gene            9866..>12939
                     /locus_tag="ACZPD4_02730"
     CDS             9866..>12939
                     /locus_tag="ACZPD4_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HIR1378026.1"
                     /translation="MRKRMKTVRGLWAGLLAAAVVLTSAAPSALTVQAEEADAVQTAE
                     VSGVEYQIYPTPHEMTYQDGGFDIGEVNIVYENGVDDVTKNRMTEVLSIKGKSESAAV
                     TNAKVDGKTNILAGIYGSDGYVDKYVKEHYTVDKSLFDHHGSYFLASNKGEIVILGLD
                     TDAVFYGITSLKHIFNQMDGTTIRNFEMRDYADVNIRGFIEGYYGLPWSNEDRMSLMR
                     FGGDYKMTSYIFAPKDDEYHKGKWRDLYPEEELAKIKEMVKVGNDSKCRFVWTAHPFM
                     GGFNQAQADQEIQALLRKFDQLYDAGVRQFGVLGDDVGSLPRTIVINMMTKVSEWAKK
                     KGDVYDTVFCPAGYNHSWQSGGTGGNYAELNEYDKGFPEDIKIFWTGEAVCKPIEQVT
                     LDHFRTHRTTDGQKRRAPLFWLNWPVNDINHGRLMMGKGVQLKTDVNPEDLAGAVTNP
                     MQESEVSKPAIFAVADYSWNIAGFNMDKSWADSFAYIDENASEELHTLAKHMSNPEPN
                     GHGLVLPESEEIQGLINTYKTQLAAGNVEEATTTQLVAEMTTIIEACDGFHAKSKNEA
                     MKAEILPFTNSLKDLCTAIRSFAQAQDAMQKNDMQSAFDLYMAGSGSYATSQTYTKEM
                     YNASPAVVTPGSTHLIPLAKTLEAALSDPMNDYVAGDVERPLKITAEASFQTWQSGKP
                     ADIIDGNSGTYAWYNGAEGVGQYYQVNFSKPTTIYGVHIENGAGKEKPDDTFGYAKLK
                     YQAEGSDEWKELENGKEYGPYAAKVDVAGLEIENVTAVRYECSRVGGSGKWPSMREFK
                     VDLQPGAADTFTKEVIRTTEGWTVYNNGNENNAIDGDEGTSVHYNVRQGDPTNGDSMI
                     AGDYFGVKLSEKITLGKIKIVQGNNDTHADYMQNCDLEWSVDGQKWTKIATFENKRTI
                     EIDVSDKDIQAQYVRIKNNATQKNWFAIREFDVDSKVWFNSKVYTNVDAYKEYRANLL
                     DESAEIEAKNDITLKKDQYIGFKIRSAQLEKVPYMLVAGDKDIENSTVSVRS"
BASE COUNT         4294 a         2307 c         3433 g         2905 t
ORIGIN      
        1 ggacagttaa agctgtgaaa acttatagaa atatgaaaag ttaaaaagcc ggaagtcgga
       61 aaaaatgtaa aaaatgaaaa gccgatgaga atacaggata aaaatatagg aaaatataga
      121 gagaaaagtg aaaattaagg attgtcggaa aacttaattt attgtataat attcgattga
      181 gaaaatgaaa tgagaacatg ggacaataca ggaggaagaa agatggcaaa aagaaatgat
      241 atccacaagg tacttatcat cggctccggc ccgatcatta tcggacaggc ttgtgaattc
      301 gattattccg gaacacaggc ttgtaaagca ttgagaaatt taggatacga gattgtattg
      361 gtgaattcaa acccggcgac aatcatgacg gatccggaga ttgctgacgt tacttatatt
      421 gaacctttga atgttgacag aatagaacaa atcataaaaa aagaaagacc tgatgcatta
      481 ctgccgaatc tcggtggaca gtccggtctt aatttatgct cagaactggc gcaggcgggc
      541 gttttggaga aatataacgt agaagttatc ggcgtacagg tcgatgcgat cgagcgagga
      601 gaagacagaa tcgaatttaa gcacacgatg gatcagctcg gtatagagat ggctcgaagc
      661 gaggttgcat attctgtaga cgaggctctt gcgattgcgg agaagcttgg atatccggta
      721 gttcttcgtc ctgcttatac gatgggcggc gcaggcggcg gtcttgtata taatgtagat
      781 gaattgaaaa cagtatgcgc cagaggtctt caggcaagtc ttgtaggtca ggttcttgta
      841 gaagaatcta tcttgggatg ggaagagctg gaacttgaag ttgtccgcga cgcagagggc
      901 aatatgatca ctgtatgctt tatcgaaaat atcgatccgc tcggcgttca tacaggagat
      961 tctttctgct ccgctccgat gcttacgatc tcagaagaag ttcagcagcg tctgcaggaa
     1021 aaatctcata aagtagtaga ttctgttaag gtgatcggcg gctgtaacgt acagtgggcg
     1081 catgatccga agacagacag agatatcatt atcgagatca acccgagaac gtctcgttct
     1141 tcggcgcttg catcgaaagc gacaggattc ccgatcgcgc ttgtatccgc aatgcttgcg
     1201 acaggcatga cgctgaaaga tattccgtgc ggaaagtacg gtacattgga taaatacgtt
     1261 cctgacggag attatgttgt gatcaagttc gcgcgctggg catttgagaa attcaaaggt
     1321 gtggaagaca agcttggaac acagatgcgt gcggtaggcg aagtaatgag catcggtaag
     1381 aacttcaaag aagcgttcca gaaagcgatt cgttctttgg agacaggaag atacggactc
     1441 ggtcatgcga aagattttga cggaaaaaca aaagaagagc ttctcaagag tttgcggtat
     1501 gcgacaagcg agcgttactt tgttatctac gaagcgctga gaaaaggagc ttctgtagaa
     1561 gagatccatg aactgacaaa agtaaaacac tatttccttg agcagatgaa agagctggtg
     1621 gaagaggaag aggctctgct tgcgtttaag ggaggtttgc cggaagacaa tgctcttatc
     1681 gcggcgaaaa aagacggatt ctctgataaa tatttaagcc agattcttga agtggatgag
     1741 gatgagatcc gcaataagag aatcagcctc ggcgttgagg aaacgtggga aggcgttcat
     1801 gtaagcggaa cgcaggacag agcatattac tattcgacat ataacggaac ggacaaaaac
     1861 ccgatcagtg aagagaaacc gaagatcatg atcttaggcg ggggaccgaa ccgtatcgga
     1921 cagggaatcg agtttgacta ttgctgtgta catgcgtctc ttgctttgaa aaagcttggt
     1981 ttcgagacga tcattgtcaa ctgtaaccct gaaacggtat ctacagacta tgacacatct
     2041 gataaactgt attttgagcc gcttacactg gaagacgtac tgagcattta taagaaggaa
     2101 aaaccggtcg gagtgatcgc gcagttcgga ggacagactc cgctgaacct tgcggcagat
     2161 ctggaaagaa acggcgtgaa gatcttaggt acggctccgt ctgttatcga tctggcggaa
     2221 gaccgcgatc tgttccgtga aatgatggat aagcttgaga ttccgatgcc tgagtcggga
     2281 atggctgtta cggtggacga tgctcttgaa attgccgaga agatcggtta tccggttatg
     2341 gtgcgtccgt cttatgtact tggcggccgt ggcatggaag ttgtatatga tgcggaaagc
     2401 atggtaggat atatgaaagc agcagtcggt gtgacacctg accgtccgat tttgatcgac
     2461 cgtttcctcg gacatgcaac agaatgtgaa gcagacgcga tcagcgacgg cacacatgcg
     2521 tttgttccgg cggttatgga acatatcgag cttgcgggag tacattccgg agattcggca
     2581 tgtattattc cttctaaaaa tatttcggaa gaaaatgtaa agacgatcaa agaatatacg
     2641 agaaaaatcg cagaagagat gcatgtgaaa ggtcttatga acatgcagta tgcgatcgaa
     2701 aacggaaaag tgtacgtatt ggaggcgaac cctcgtgcgt caagaacagt gccgcttgta
     2761 tcgaaagtat gtaatatccg catggttccg cttgccatcg acattgtgac aagtgaactg
     2821 acaggaagac cttcaccggt tccggcgtta aaagagcaga acattccgta ctacggagta
     2881 aaagaagcgg tattcccgtt caatatgttc ccggaagtag acccgctgct cggaccggag
     2941 atgagatcga ccggagaagt gctcggattg tccaaatctt tcggcgaagc attttataag
     3001 gctcaggaag caacacagac aaagcttccg cttgaaggta cggtgctgat ctctgtaagc
     3061 aatcgtgata agccggaagt tgtggaagtt gcggctgatt ttgcaaaagc gggattcaag
     3121 atcattgcgt cagaccatac ttgcagcctg cttaaagaga acggaatcga agcagagaag
     3181 atcaataagc ttcaggaagg ccgtccgaat atgcttgatg tgatcacgaa cggaaaagtt
     3241 gacatgatcg tgaatacacc gtcaggaaaa gaaagtaaga atgacgacag ctatttgaga
     3301 aaagctgcga tcaaaaagaa agttccgtat atgacgacaa tggctgctgc gaaagctgcc
     3361 gcaaacggaa ttcttgcagt gaagaaacat ggaagcaaag aagtgaaatc acttcaggaa
     3421 attcacggag aaattacgga caaagaatag tatgattcat taatataaga caggttttat
     3481 gaagatgccg gccgcatttg cggttaggca tcttttgtat aaaatgaaag atattttcct
     3541 ttggcaaatc aatcagatgg tgtatatgtg aaaaaatcaa taaaaataaa gctaaaatag
     3601 ccatattata caaaaagcat agataaaaag aaaaaaatac aaaagaagtg ataaaaagtg
     3661 taaatattat tggggggggg gtaatgctat catttataag atgatgggag taatgtggtg
     3721 agcacattcg tacattacat tttaaaaaga aggagagaag tttatgaaaa agagaatgtt
     3781 ccataaggga atggctgcgg ttctcgcggt atcgctgaca accggcggag cccttccgtt
     3841 tatggctcag acagttcatg cggaagatac tgcggcagag cagggtcaga tacctgagaa
     3901 gaaaagcggt gtcgtaacgc ttgaaaagac tgacggctct tatgttttcg gaaatgaata
     3961 tttaaaaaga acgttcagtt tgagcgcgga caaagtattg tcaacaaaag agattacaaa
     4021 ctatcgtacc ggaacgactc cgactgtgtt cacaccgcag gcgggcagtg aagagtttat
     4081 aatcaacacg cttgacaata attccgaagg ggaagacagc ggattcgtag cgcctaagaa
     4141 aaaattagat accaatggtt ggacagcaga ggcggacagc gttgcgacta atgaaggagc
     4201 gaatggcgga gcggataaga tgtttgatgg aaagaatgat acttattatc attctaaata
     4261 taacgaaggc acagatgccg aacggaaata tccgcataat atttatgtcg attttggtgc
     4321 agagaagtcg ttccagtctc ttcgctacca gcagagagta gatgctcaag gaaatccgac
     4381 agtgagcgga catgtaaaaa gttataaaat ctatacagga gacagtattg atgcgttaaa
     4441 acaggcaaca gatgcacagc cggttgcaga aggttctttt gataataaaa aagaaacata
     4501 tgtaaatttg aaagagaagg ttacggcaaa atgtgtgcgt atcgagtttg ttgactgtca
     4561 tgacccgtca gattcaaatg taagtaaaga cgtagcctgc tgttcggaat ttgatttctt
     4621 tgaggatact gcgacgttcc cggaggcgac ggacgatgcg acacagctta agacgagcga
     4681 gatgaaagtg cagggcgagc cggagctgac agaaaaggac ggagtgaaaa cgctgacctt
     4741 tacgtttgaa ccgaagagag taagaggtgt agattacacg atcaaagaag tgatcacgat
     4801 gaaagacgga gattctttca tgagaaagcg cctcgatatt tctgttgggg aaggacaggc
     4861 agaaaaagct aaaatcgatt atatcgattt ggaaaatatg cagatcagcc aggatgattt
     4921 gaaaaaagat gaatactggt cgatcaaaga taatatggca gacaatcctg acatgggcgg
     4981 aatgaaaggc gattatctgg agcttggaca gccgtactat gtaggtgcga tgtactgggg
     5041 atgtgagttc ccggagacag agaacaaaat taaaggttct aattccttta tccgttacta
     5101 ctatggaaaa tcattgaaat ccgatgataa gtttgagtat aacgaaggaa acgaaaacgg
     5161 aaagatgacg acgtgggacg ctgtcgtagg agctgcccga agcagagatt acagcgtgac
     5221 acagagcgat ttctatgaat atatcgagac gatcgcaata gatacggagt tccgtcagca
     5281 gtacaactca tggtacgaca acatgaagga gatcacggac gagattattc agaagagttt
     5341 cttcgagatt gagaaaggat tcacacagta cgggatagct cctcttgatt cttatgttgt
     5401 agacgatgga tggacgaatt acagctcctt ctgggatttt aataataagt ttccgaatga
     5461 gctttacaac tccagtctgc aggtgaatca gcttgcgtcc aacttcggac tgtggcttgg
     5521 accaagaggc ggatatggaa cagaaagaac gattgcgaat tggattgcaa gtaatggttt
     5581 gggttctgta aataatcaat cgggtggaga tattaatatt tctgatgcga gatatctgac
     5641 gaaattgaat aaagatgttt tctgcgagta tcaggataag tttgatatca actactggaa
     5701 actggatgga atgctcttaa atccatcgac agagcagtcc gaatattatg tgacaggtaa
     5761 tccgctttat acgatttcag agacatacga aagatggacg gatatttttg aagatatgcg
     5821 tgacaacaga gcgggaaaag acctttggct taatatgact tcttacacaa acccgagtcc
     5881 gtggcacgtt cagtgggtga acagcgtatg gatgcagaat acgggagata caggttatac
     5941 ggatagcttt aatgcaacgg atgaagaagc gatgcttaca taccgtgaca acgcttatta
     6001 taacttctta aatgaaagag agtggcagct tccgaacaaa tatttttata accatgaccc
     6061 ggtttacgga ttgactgcaa acgatgctta tcatagaccg gatatcaaat atacagatga
     6121 tgagatgaga aatcacctgt atatgctcgg aacgagagga acggcattct gggagtatta
     6181 ttatagttac tccatgtttg atgacaataa atggcagatc aacgcggaag cggcaaaatg
     6241 gatcgaagac aatttcgaca ttcttcagaa atcacagatg ttcggcggaa aaccgaatga
     6301 cggaaatgtt tacggatatt cctgctggaa cggaaaagag ggaattctct cgatcagaaa
     6361 tccgaagaat gaagcacaaa gttataaagt aacttatgac agactgatcg gcgtcggaga
     6421 agatctcgga actgtttacg gaaaggttgt cgtaggagat cagagacacc agacagatga
     6481 accgcttaca tatggcaagg aagtgacata cacgcttaat ccgaaggaag ttttgatcct
     6541 gcagtttggt gagaaagatg aaactcctgc gaaaatcctt tctgtagaag gaaacggaaa
     6601 agaagcagaa gttgaatttg acgagacgat ccgtacgccg gaagcgggaa tgtttaaggt
     6661 agacggatac gaagtgacaa aggcagaatt gaaagccgac agacgtacag tgaaactgac
     6721 tttggataag gaactgaaag atgcccggac agtgagcgta agtgtagacg gcgtgaaaga
     6781 tactgtcgga aatacttcga aagtaagcgc gcagaatgat gcgtttaaag acggcatcat
     6841 aacaggagtg atcagcgatg atctgaaaga cggagctgta tcgacaaaag cgaaatactc
     6901 tgtcgacgga cacggcggat ttactgtaac aggtaagatc aagacagatt caaaagacgt
     6961 ggtacttgca gaacagaaag gcgcttataa agtcggtatt gacggcgaag gatatctgac
     7021 gtttgagttt aacgatatga agatcacatc caaatatgat cagaagacgg ttgataaagc
     7081 aaacgacagc tacacaagcg agacgaaagg aattgcggct gacggtaaag agcatcaatt
     7141 ctctgctgta aaagaaatta acggaatgat caaactttac cttgacggaa aagtggttgc
     7201 ttcaacatac agcgaagata aagcaaatcc ggagatcgca aaaggcgaaa cgatatttgc
     7261 gcagggtctg acaaaagacg aagtttctta tattacagtt ctcgacagat cgcttgcata
     7321 tgatgaagtg aaagatctga tcgatacaga agataatgtt gttcttgcaa agaacaatcc
     7381 gaaagtaaaa gttaccgctt atgatgcaac tgttaatacg gcagtggcag agaaaccgga
     7441 cagaccgttt agcatggtaa acgacggcgt gaagagtacg gcgaattatc tggagctgac
     7501 agatacgagc gacagccaga accattcccg ttacgtgcag ttcgatctcg gagacgagta
     7561 tgatctgatg aagatccata tgacaagata ctgggatggt tcaagaaaat acggaccgac
     7621 agtgatccag ttaagtacag acgagaactt tgcggcagac aaaacgacaa cggtatataa
     7681 ctccgataaa gacaatgtac ataaacaggg cgcgggcaaa gatgagtttt atgtcgagac
     7741 ggcagcaggc aaagagatgt ggaacgcaga aaacagtgaa cctgtaacag ctcgctacat
     7801 ccgtgtctat gtaaacggaa gagaaaataa tcagggtacg tctgatcata tcgtcgagtt
     7861 cgaggcttac ggtgcgaaag acggcggagc gatcatccgt ccggacagac cggaagagcc
     7921 ggagaaaccg gtacttacag gcgtgacagc gtcagttgaa aaggcagaac tgaaagtcgg
     7981 cgagacaaca aaggcaacag caacgatcat gccggaaggt gcagaaggcg tagagcttgc
     8041 atggacgtct tccgatgata agattgcaac agtagataag gacggaaacg ttaaggcggt
     8101 tgcagaagga acggcaacgc tgactgtaac ggcgacacag ggaagcggag acggcgcggt
     8161 aacaaaaaca gctactgtgg atgtaacggt aataaaagac ggcggaacag atccggaacc
     8221 ggagaaaccg gtacttacag gcgtgaaagc gtcagttgaa aaggcagaac tgaaagtcgg
     8281 cgagacaaca aaggcaacag caacgatcat gccggaaggt gcagaaggcg tagagcttgc
     8341 atggacttct tccgatgaca agattgcgac agtagataag gacggaaacg ttaaggcggt
     8401 tgcagaagga aaagcaacgc tgactgtgac agcgacacag ggaagcggag acagcgcggt
     8461 aacaaaaaca gctactgtgg atgtaacggt aataaaagac ggcggaacag atccggaacc
     8521 ggagaaaccg gtggtactta caggcgtgaa agcgtcagtt aagaaagcag atctgaaagt
     8581 cggcgagaca acaaaggcaa cagcgaagat cacaccggaa aaggcagaaa atgtaacatt
     8641 tgcatgggct tcttccgata aaaaagttgc aacggtagat gctgacggca atgtaaaggc
     8701 agtcggcaaa ggaacagcga agctgacagt gacagcgaca cagggaagcg gagcggatgc
     8761 agtgaaagta actgacactg tgaaagtaac ggttacaaaa gacggcggtc agacaccgca
     8821 ggaggatgga aagacacctc cgaagacagg agacgaaaca gcgccgttct tcccgtttgt
     8881 acttgcactt gcggcaggcg cagtggtcat gatcacgaga aaaaagaacg gataaacaaa
     8941 gatacgcaga aaagatcaaa ataaaaaatt gatacttaaa aaggattgtc gtgtgagcgg
     9001 cgatcctttt ctgcattatc ggatattccg gtttctgatc gcgcagaacc ttcagggagg
     9061 acgtctgcgc cgcagcaggc aagtttacag ctttacggcg tgtggaaagg aaacgtatat
     9121 aaaaatgtta gaatggaaaa aacggttcgc tttgtgataa aatgatagaa gaaaacaact
     9181 tcggatcatt gaaatgggaa aacgcagaag gaatataatg aaaagtatta tgaggaaatg
     9241 ctgtcggtcg ttcgggaata caaggattac agcgtgcttg ggcatatgga tctgatcgcc
     9301 cgttatgatg aaaagggcgt ctatccgttt gaaaagatca aaccgatcgt cgaagagatt
     9361 ttgcaagttg tcatagcgga tgggaaaggt ctggaagtga atacatcgtc atatcggtac
     9421 ggcctttctg acacgacgcc ttctgtggaa attttaaaac ggtacagaga gcttggcgga
     9481 aagatcgtca caatagggag cgacagtcat aaaccggaac acttgggcgc ttatatagaa
     9541 gaaacgaagg aaatgctgag aaaagcaggt tatacgcagt tttgtactta tgaaagaatg
     9601 tctccggttt ttcatgatct ataacaacgt ttttgctata taaactgcaa agaaagcgga
     9661 gagaaaagag ataattgtgc agtataaaca aaataaagaa aatagaataa acatattgca
     9721 caaaagaagt ccgggcaata ggcgaaaact atcataaatt acaaatcggt tgctaaaaag
     9781 tgcaaataac tgaaatgtaa agtgcgatat cataagaaaa taggaatagt gggcaactat
     9841 agtaactaga aaaagaggga aatgtatgag aaagagaatg aaaacagtaa gaggcttgtg
     9901 ggcaggtctg cttgcggcgg cagttgtact tacatcggct gcaccgtcgg cgttgaccgt
     9961 acaggcagag gaagctgacg cagtgcagac tgcagaagtt tcaggggtgg aatatcagat
    10021 ctacccgact ccccatgaga tgacttatca ggatggggga ttcgacatcg gagaagtgaa
    10081 tattgtttac gaaaacggtg ttgatgatgt gacaaagaat cgaatgacag aagttctttc
    10141 gatcaaaggc aagagtgaga gcgctgctgt taccaatgca aaagttgacg gcaagacgaa
    10201 tatccttgca ggtatctacg gctccgacgg atatgtagat aaatatgtga aggagcatta
    10261 cacagtagat aaatctttgt ttgaccatca cggttcctat ttcctcgcat ctaataaggg
    10321 tgagatcgta atcctcggat tagatacaga tgcggtattt tacggcatca catctttgaa
    10381 gcatatcttc aatcagatgg acggaactac gatccgcaac tttgagatga gagattatgc
    10441 agatgtaaat atccgtggat ttatcgaagg atattacgga cttccgtggt cgaatgagga
    10501 cagaatgtct cttatgagat tcggcggaga ttataagatg acgtcttata tctttgctcc
    10561 gaaagacgat gagtatcaca aaggaaaatg gcgtgattta tatccggaag aagaactggc
    10621 aaagatcaag gaaatggtaa aagtcggaaa cgattctaag tgccgtttcg tttggacagc
    10681 ccacccgttt atgggaggat ttaatcaggc gcaggcagat caggagattc aggcgcttct
    10741 gcgcaaattt gatcagttgt acgatgcagg cgtgcgtcag ttcggagtac tcggcgacga
    10801 cgtcggaagc cttccgagaa cgatcgtgat caacatgatg acaaaagtat ccgagtgggc
    10861 aaaaaagaaa ggcgatgtat atgacacagt attctgtccg gcaggatata accattcatg
    10921 gcagtcaggc ggtacaggcg gcaattatgc agagctgaac gagtatgaca aaggattccc
    10981 ggaagatatc aagatcttct ggacaggaga agcagtatgt aaaccgatcg agcaggtaac
    11041 gcttgatcac ttcagaactc atagaacgac agacggtcag aaacgtcgtg ccccgctatt
    11101 ctggctcaac tggcctgtaa acgacattaa tcacggacgt cttatgatgg gtaaaggtgt
    11161 gcagcttaaa acagatgtaa atccggaaga tcttgcggga gcggtgacaa acccgatgca
    11221 ggaatcagaa gtatcgaaac cggcgatctt cgcagttgcg gattatagct ggaatatcgc
    11281 aggatttaat atggataaga gctgggcgga ttcatttgcg tatattgatg aaaatgcatc
    11341 tgaagagctt catacacttg cgaaacatat gtctaacccg gaaccgaacg gacatggact
    11401 tgtattgccg gagtcagaag aaattcaggg tctgatcaat acttataaaa cacagcttgc
    11461 agcaggaaac gtagaagaag caacaacaac gcagcttgtg gcagagatga caacaatcat
    11521 tgaagcttgc gacggtttcc atgcaaagag caaaaacgag gcaatgaaag cggaaatcct
    11581 tccgtttaca aactctttga aagatctctg tacggcaatc cgttcttttg cacaggcaca
    11641 ggatgcaatg cagaaaaatg atatgcagag cgcgtttgat ctgtatatgg caggttcggg
    11701 aagttatgcg acctcccaga catatacaaa agagatgtac aatgcgtctc cggcagttgt
    11761 aactccggga tcgacccatt tgatccctct ggcaaaaaca ttggaagcgg cattgtcaga
    11821 tccgatgaac gattatgtgg caggcgatgt tgaaagaccg ttgaaaatta cggcagaggc
    11881 aagctttcag acatggcaga gcggaaaacc ggcagatatt attgacggca acagcggtac
    11941 gtatgcatgg tacaacggag cagaaggtgt cggacagtat tatcaggtga acttcagcaa
    12001 accgacgact atttacggcg tacatatcga aaacggcgca ggaaaggaga aaccggacga
    12061 tacattcgga tacgcaaaac tgaaatatca ggcagaaggt tctgatgagt ggaaagaatt
    12121 agaaaacgga aaagaatacg gaccatatgc tgcgaaagtt gatgtggcag gacttgaaat
    12181 cgagaacgtt acggcagttc gttatgagtg ctcgagagtg ggaggatctg gtaaatggcc
    12241 ttctatgcgt gagtttaaag tagacttgca gcccggagcg gcagatacgt ttacaaaaga
    12301 agtgatccgt acaacagaag gttggactgt ttacaataat ggaaacgaaa ataatgcaat
    12361 cgacggtgat gaaggcacaa gcgtacatta caatgtaaga cagggagatc ctacaaacgg
    12421 tgactctatg atcgcaggtg attatttcgg agtgaagctg tccgagaaga tcacacttgg
    12481 aaagatcaag attgtccagg gtaataatga tacacatgca gattatatgc agaactgtga
    12541 tctggagtgg tcggttgacg gtcagaaatg gacaaagatt gcaacatttg aaaacaaacg
    12601 cacgatagag atcgatgtat ctgacaaaga tattcaggca cagtatgttc gtatcaagaa
    12661 taatgcgaca cagaaaaact ggtttgcgat cagagaattt gacgttgact ctaaagtatg
    12721 gtttaactcg aaagtttaca caaacgtaga cgcttacaaa gaatacagag caaatttgtt
    12781 ggatgaaagc gctgaaatcg aagctaagaa tgatattact ttaaagaaag atcagtatat
    12841 cggcttcaag atccgttcgg cacaacttga aaaagttcct tatatgcttg ttgccggcga
    12901 taaagatata gaaaacagca cggtttccgt acgttcaag
//
LOCUS       DBMDXF010000038        12451 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1465_length_12451_cov_2.243320, whole genome shotgun sequence.
ACCESSION   DBMDXF010000038 DBMDXF010000000
VERSION     DBMDXF010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12451)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12451
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_12451_cov_2.243320"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="ACZPD4_02735"
     CDS             <1..552
                     /locus_tag="ACZPD4_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1378027.1"
                     /translation="VIAEAKEGYEFVNWTSEGKVFSDSAAYEFAVEKDMELTANFKAK
                     EEPKPPVTEDTFKLNVTVNDDKMGTVKLDPAKESYKAGEIVTATATAKDGYKFVNWTV
                     DGKEVSDKAEYAFKVDRDVTLKANFEKVAADPKPETPKPENPKDEDKAVQTGDNGVSP
                     IIPLAGLALAAGAAVTVLRKKED"
     assembly_gap    679..778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_binding    1183..1459
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1758..4403
                     /locus_tag="ACZPD4_02740"
     CDS             1758..4403
                     /locus_tag="ACZPD4_02740"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR1378028.1"
                     /translation="MSKELAKTYDPKAIEEKLYEKWCENKYFHAEVDRSRKPFTTVMP
                     PPNITGKLHMGHALDNTLQDILIRYKRMEGYNALWIPGTDHAAISTEVKVTNQLKEEG
                     IDKKELGREGFLERTWQWKEEYAGTIENQLKKLGISCDWDRERFTMDEGCSKAVEEVF
                     IKLYEKGYIYKGSRIINWCPKCKTSLSDAEVEHEEQEGHFWHIKYPIVGTDRFLEIAT
                     TRPETLLGDTAIAVHPDDERYQDIIGKNVILPLVNREIPIVADYYVDKEFGTGAVKIT
                     PAHDPNDFEVGKRHDLEEINVMNDDATINEKGGKYAGMERYEARKAIVKDLEEQGYLV
                     KVVPHTHAVGTHDRCGTTVEPLIKQQWFVKMEELAKPAIEALKSGELKFVPERFDKIY
                     LHWLENIKDWCISRQIWWGHRIPAYYCDECGEFVVDRHAPEKCPHCGCTHFTQDEDTL
                     DTWFSSALWPFSTLGWPDKTEDLDYFYPTDVLVTGYDIIFFWVIRMVFSGYEHTGKSP
                     FHTVLIHGLVRDSQGRKMSKSLGNGIDPLEIIDKYGADALRLTLVTGNAPGNDMRFYN
                     ERVEASRNFANKVWNASRFIMMNMDENIIDEPSHDLFTPADRWILSAANTLAKDVTDN
                     MDKFELGIAVQKVYDFIWDEFCDWYVEMAKFRIYHKEEAPEAANCALWVLKTVLAQGL
                     KLLHPFMPFITEEIYGALVPEEESLMMSEWPKYKEEWNFAQDEFVMERVKAVTRGIRN
                     IRAEMDVPNNRKTNVFIVSEKEELTKAIEGFKQSVMPLMLASDIIVQSTKEGIEDNAV
                     SIVVPDAVVYLPLEDLVDFEQEKERLTKEEERLNKEIKRAKGMLANEKFVSKAPEAKV
                     QEERDKLEKYEQMLAQVKERMVGLG"
     gene            4396..6594
                     /locus_tag="ACZPD4_02745"
     CDS             4396..6594
                     /locus_tag="ACZPD4_02745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1378029.1"
                     /translation="MANIKGFFTKVRNLKKEDIKEHFKKRKERRQMILEKRRNGKFAK
                     KMQPVYKWMNRLSVPLQFLLACIINFFIEVISRLSFGEAWAYLTKTPLVFLYNSCMIF
                     VTFTIVYLVRRRVFTRILLSVFWLFLGICNGYLLTKRVTPFNAQDLKVLSDALELTGN
                     YFNTFELIMIIIGVTALIVWVVSMWKRGGQYTGKMHRWIALGGVVASFVGCMALTNLA
                     IDKRVISNYFGNIAFAYEDYGFPYCFSASLFNTGINEPTGYGKETMEKINEEEKLTKS
                     STGRSEEQRPNIIFVQLESFFDPTEVEWLRFSEDPIPNLRKMFSEYSSGYFKVPSVGA
                     GTANTEFEVLTGMSMRFFGPGEYPYKTYAKTRTLESAASALKGLGYGAEALHNNGGNF
                     YSRAQVFNNMGFDHYTSKEFMNILQTTPKGWATDDILVPNIMESMDTTEGQDFVFTVS
                     VEGHGEYPTEKVLEDPEIVVSGVEDEGERNAWEYYVNLVHAMDEFAGDLVRAVEERNE
                     PTVLVFYGDHLPTMNLEAKDLKSRYLYNTNYVIWDNIGLEKKDGNVAAYQVMADVFER
                     LDIHAGTVFNYHQQRRHTKNYLADLELLQYDIMYGDQYVYAEEGSPMKEGHMYMGVKD
                     AVISQVTEQYNKTYSIYGENFTKQSKVFINGEKQKTTFLNNTRLDLKESKLSDGDTIM
                     VAQVGSSNTTFRTTKTYKYNAGTLTEMPPEKDAPENGRQAFVVEKEEKEK"
     gene            6591..7895
                     /locus_tag="ACZPD4_02750"
     CDS             6591..7895
                     /locus_tag="ACZPD4_02750"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1378030.1"
                     /translation="MTYSEAVDYILDIPKFTKKNDAAHTKAFLKTLGDPQRKMRIIHV
                     AGTNGKGSVCAYLDGMLRSEQKRTGLFTSPHLVKINERIVIDGQMISDERFLEVFEET
                     MQAVDKMKEAGLSHPTFFEFLFGMAVLAFAESGVEYAVLETGLGGRLDATNAVESPAA
                     SVITSIGFDHEQYLGDTLEKIASEKAGIIKPHVPVYYAQTTGESDSVIEKEAKKRGSF
                     CKKIGKDAYEILGIEDKHIAFSCLNDYYGTTTWKLNNIGLYQPHNALLAMEVMRGLFG
                     ENGHPHLWKEALEAVRWRGRMEEVLPGVYVDGAHNISAVKAFAETVEKAGFADAVLFS
                     AVRDKDYEEMIKYLCEHTEAACYIVTQIEDARGTRADELGVVFRKYTDKPVRVIGSVK
                     DAFEYAVSSRRGGNVYCLGSLYLTGMIEGYLGSRENQIRGGR"
     gene            7897..8031
                     /locus_tag="ACZPD4_02755"
     CDS             7897..8031
                     /locus_tag="ACZPD4_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378031.1"
                     /translation="MLDYEEELKKFKPSLEVDDIENAVYQEDLTDMTDLLKQVMDQKK
                     "
     gene            8052..9374
                     /locus_tag="ACZPD4_02760"
     CDS             8052..9374
                     /locus_tag="ACZPD4_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1378032.1"
                     /translation="MEYTKKIVYQSNYWYNDGLRKAKIRDMSGAIMSLRKSLQFNREN
                     IAARNLLGLVYYGIGEVPEALVEWIISKNLKSRDNIADYYIKNVQSSAKELEQIDLAI
                     KKYNQCLTYCRQNGEDLAAIQLKQVIAAHPTFLKAYQLLTLIYLHTGQYSQARQTIKT
                     ARKLDTTNEITLLYMHELTKKGKKKTKEEKKKEEAVEYSLGNETIIQPKHSRFRQVTA
                     QLAVANIFIGAAIGAAIIWFLVAPAVNQSRSERMNDQMREYADEIKSLEAQVSAQTRT
                     LDNYRASGEDAQANAELAQKTAEGYEKLLSVEGQFLSNDYDDAALADALLGISRDTLK
                     QTGQVKYDEIAAAVYPGACEVKLAEGTQALNSGDYAGAIDPLSKVVLMNEGYNDGQAL
                     LNLAQAYKGSGDNENATVYFQKVIEKYAGSEYAAEAQSGLDEITNENN"
     gene            9557..9871
                     /gene="spoIIAA"
                     /locus_tag="ACZPD4_02765"
     CDS             9557..9871
                     /gene="spoIIAA"
                     /locus_tag="ACZPD4_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HIR1378033.1"
                     /translation="MEYQVQENCLTIYLPAEVDHHNAEEMKRKADKLIDRNHIKYVIF
                     DFENTDFMDSSGIGVIMGRYKTISLIGGEVWAVHTNARIRKILTLSGVTKIMQIYEEE
                     EE"
     gene            9868..10323
                     /gene="spoIIAB"
                     /locus_tag="ACZPD4_02770"
     CDS             9868..10323
                     /gene="spoIIAB"
                     /locus_tag="ACZPD4_02770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR1378034.1"
                     /translation="MTPANEMELKFDSRSANEGFARVAVAAFMTQLNPTVEEVADVKT
                     AVSEAVTNVIIHGYEKEVRKVYIKCMIEENIFTVEVCDQGKGIENIEEAMCPMYTTRP
                     DQDRSGMGFAFMEAFMDSVEVESELGKGTRVRMKKAVGKGRDIWTTQSL"
     gene            10302..11021
                     /gene="sigF"
                     /locus_tag="ACZPD4_02775"
     CDS             10302..11021
                     /gene="sigF"
                     /locus_tag="ACZPD4_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HIR1378035.1"
                     /translation="MDDTIALIRKSHSGDKAAREQLVKENVGLVHCVVKRFLERGAER
                     EDLFQIGSIGLLKAIDKFDLTYNVKFSTYAVPLISGEIKRFLRDDGMIKVSRSLKEIA
                     YKSVQARERLISSLGRDPTIEELSEEVGVEREELVQAMEAGCEIESIYRPVHQKEGSE
                     IRLLDKLEEKEKREDKILDLIVLKELLETLDEEEKQLIFLRYFADRTQSDVGKIMGIS
                     QVQVSRSEKRIIEKLRKKGGR"
     gene            11111..11296
                     /locus_tag="ACZPD4_02780"
     CDS             11111..11296
                     /locus_tag="ACZPD4_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378036.1"
                     /translation="MMEDGNGRKKVWICLSIVVLAAVVIGAVYYMNMPKEHSEEGFLI
                     RRESEDCADGIRENALY"
     gene            11268..11930
                     /locus_tag="ACZPD4_02785"
     CDS             11268..11930
                     /locus_tag="ACZPD4_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HIR1378037.1"
                     /translation="MASGKTLYIKGDRDVEVTKPDVTLADLLSMECSDQSLLPKLKTM
                     KILKFKKTGNQRCVISVLKIIACIHGQYPDMEVQNLGETDMIVTYEDQKTPSYAWHLT
                     KTAFVTAVTFCGAAFSIMAFNNDVDVTKLFDQIYHLVTGNKPGGITILEIAYSAGLMI
                     GILIFFNHFGKRRFTVDPTPMEVQMRVYENDIQTTLIENAARKEKEIDVGKGGGIHGT
                     GG"
     gene            11914..12378
                     /locus_tag="ACZPD4_02790"
     CDS             11914..12378
                     /locus_tag="ACZPD4_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HIR1378038.1"
                     /translation="MGQAAEVIVEQLVLALVGLSAGAAVAGGLFSFIVELGVIADFAD
                     RTHTGDKLLFYETCVALGGIIGNIVFLFFSFAPNEVSFASASFASGAVQVLFGIFAGI
                     FVGCWSMALAEILNVFPIFMRRARIVRYASVFVIGAAIGKGLGAGLFFLKGW"
BASE COUNT         3841 a         2231 c         3357 g         2922 t
ORIGIN      
        1 gtgatcgcag aagcaaaaga aggatatgag tttgtaaact ggacatcgga aggtaaagta
       61 ttctccgatt ctgcagcgta cgagtttgct gtagaaaagg atatggaact gacagcgaac
      121 ttcaaagcga aagaagaacc gaaaccgccg gtaacagaag atacattcaa actgaacgta
      181 actgtaaacg acgacaagat gggaacagta aaacttgatc ctgcaaaaga aagctataaa
      241 gcaggagaaa tcgtaacggc aactgcaacg gcaaaagacg gatacaagtt cgtgaactgg
      301 acagtagacg gtaaggaagt ttccgataaa gcagagtatg catttaaagt tgacagagat
      361 gtaacattga aagcaaactt tgaaaaagta gcggcagatc cgaaacctga aactccaaaa
      421 ccggagaatc cgaaggatga ggacaaagca gttcagacgg gagacaacgg cgtatcaccg
      481 atcattccgc ttgcaggatt agcattagcg gcaggcgcgg cagttacagt tcttcggaaa
      541 aaagaagact aatcgccgga tattcaaact gatgagaata atttgaaatc agaatgaaaa
      601 aataaagatt taaaacagtg ggacggcgct gatctatttt acggatcagc gtcgtttctc
      661 tattgccgcc aaagtgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
      781 agttccgccc gcgccattcg taaaaccgca ctgcgtgctt attgtggctg ccgccactgt
      841 tttactcata aaaaggcgct cagcacatac agatgtccgt tcgcaaaaac atgtaagcat
      901 gttttgtgct tcccgaacac tgtatggcag gtttagttga gagcaacagt tccgcccgcg
      961 ccattcgtaa aaccgcactg cgtgcttatt gtggctgccg ccactgtttt actcataaaa
     1021 aggcgctcag cacatacaga tgtccgctcg caaaaacatg caagcatgtt ttatgcttcc
     1081 cgaacactgt atggctgaac tgttgcgtta aagtcttaag attctctaaa aatcttgaca
     1141 aatacggcgc ttatgtctat agtatgtggt agttatttta aggtaaaggt attgatgaag
     1201 aggagtacat cattccaacc gccagagaga actgcccgga aaattatagg taagatcgcg
     1261 tgagatattg cgtgaaagca aaaagagatc cggctgaaag gcagtttcgg aaaaaggatg
     1321 atggaaggta gcttctgaac cggatgattg aacgattgaa gtaggttatc ccggagtagt
     1381 tcccgttata gaatgtgaga gatatatgtg tgacagcata tatgaaataa ggtggtaccg
     1441 cggagaattc gccctttaca gatgtaaagg gcgttttttg tataggcgac gagccaaagt
     1501 ccgggcttgc ccgagacctt ggcgaccgct tacaatcccg gaatgtgccg tttggcactt
     1561 ccggtgattg ttgccaagca tgcgataaaa cagtccggtg gactgttttg agtatcggcg
     1621 gccggtcaaa gtcgggtttg tccgaaattt tggcgcgtgc aaagtgccaa gcccctcact
     1681 tatgaggggc ggagagttga agtgggcttg ctcactttgt tcaataaaat gagggaaagc
     1741 aaagaaagga aaaaagaatg agcaaagaac tggcaaagac atatgatccg aaagcgatcg
     1801 aagaaaaatt atatgagaag tggtgtgaaa acaaatattt tcacgctgag gttgacagga
     1861 gcagaaaacc gtttacaacg gtgatgccgc ctccgaacat taccggaaaa cttcatatgg
     1921 gccacgcgct tgacaataca ttgcaggata ttctgatccg ctataaaaga atggaaggat
     1981 ataatgcact gtggattccg ggaacagacc atgctgcgat ctctacagaa gtaaaagtta
     2041 cgaatcagct caaagaagaa ggaatcgata agaaggaact cggcagggaa ggatttctcg
     2101 agcgtacatg gcagtggaaa gaagagtatg cgggaacgat cgagaatcag cttaaaaagc
     2161 ttggtatttc ctgcgactgg gacagagaac gcttcacaat ggatgaggga tgctcgaaag
     2221 cagttgaaga agtgtttatc aaattatacg aaaaagggta tatttacaaa ggttcaagga
     2281 tcattaactg gtgtccgaaa tgtaagacgt ctctttcgga tgcagaggta gagcatgaag
     2341 agcaggaagg acatttttgg catattaaat atccgattgt aggaacagat cgtttccttg
     2401 agattgcgac aacgcgtccg gagactcttc ttggcgatac ggcgatcgcg gtacatcccg
     2461 atgatgagag atatcaggat attatcggca aaaacgtgat ccttccgctt gtaaacagag
     2521 agattccgat cgtagcggat tattatgtag acaaagagtt cggaacagga gcggtgaaga
     2581 tcactccggc acatgacccg aatgattttg aagtaggaaa acgtcatgat ctggaagaaa
     2641 tcaatgtgat gaacgatgat gctacaatca acgaaaaagg cggaaaatat gcaggtatgg
     2701 aacgctacga ggcgagaaag gcaatcgtaa aagatctcga agagcaggga tatctcgtga
     2761 aagttgttcc ccatacacat gcggttggaa cacacgacag atgcggtacg acggttgagc
     2821 cgctgatcaa acagcagtgg ttcgtgaaga tggaagagct tgcaaaaccg gcgatagagg
     2881 cgctcaaaag cggagaatta aaatttgtgc ctgaacgttt cgataagatt tatcttcact
     2941 ggcttgaaaa tatcaaagac tggtgtattt ccagacagat ctggtgggga cacagaattc
     3001 cggcatatta ctgtgacgaa tgcggagaat ttgtcgtaga cagacacgct ccggagaaat
     3061 gcccgcattg cggatgcacg cattttacac aggatgaaga tacgcttgat acatggtttt
     3121 catcagcgct ctggccgttc tctacactcg gctggcctga taagacggaa gatcttgatt
     3181 acttctatcc gacagacgtg cttgtgacag gatacgacat catttttttc tgggtgatcc
     3241 gtatggtatt ctcaggatat gagcatacag gaaaatctcc gttccacaca gtactgatcc
     3301 acggacttgt aagagattca caaggccgaa agatgagtaa gtctttagga aacggaatcg
     3361 atccgctgga gatcatcgac aagtacggcg cggacgcact tcgtctgact cttgttaccg
     3421 gaaatgcacc gggaaatgac atgcgtttct ataatgagag agttgaggca agccgtaact
     3481 ttgccaataa agtatggaac gcatcccgtt tcattatgat gaatatggat gaaaatatta
     3541 ttgacgaacc gtctcatgat ctgtttactc cggcagacag atggatctta tcggctgcga
     3601 atacgcttgc aaaagacgtg acggacaata tggacaaatt tgagcttggt attgcggttc
     3661 agaaagtata tgactttata tgggatgagt tctgcgactg gtatgtagag atggcaaaat
     3721 tccgtattta ccacaaagag gaagctccgg aggccgcaaa ctgcgctctc tgggtattga
     3781 aaactgtgtt ggctcaggga ttgaaactgc ttcacccgtt tatgccgttt ataacagaag
     3841 agatttacgg ggcgctcgtt ccggaggaag agtcgcttat gatgtccgag tggccgaaat
     3901 acaaagaaga gtggaatttt gcgcaggatg agtttgtcat ggaacgcgtg aaagcggtga
     3961 caagaggcat ccgaaacatc cgtgcggaga tggacgttcc gaacaaccgt aaaacaaacg
     4021 tattcatcgt cagcgaaaaa gaagaactga caaaagcgat tgaaggattt aaacagtcgg
     4081 taatgccgct tatgttggcg tccgacatca tcgtgcagag cacaaaagag ggaatagagg
     4141 acaatgcggt atccatcgta gttcctgatg cggtcgtata tctgccgctt gaggatcttg
     4201 tggatttcga gcaggaaaaa gagagactta caaaggaaga agagagactg aacaaagaga
     4261 tcaaacgtgc gaaaggaatg cttgcaaacg agaagttcgt aagcaaagct ccggaggcaa
     4321 aagttcagga agagagggac aaactcgaga aatacgagca gatgctcgca caagtaaagg
     4381 agagaatggt aggtcttggc taatataaaa ggttttttta caaaagtcag aaatctgaaa
     4441 aaagaagata ttaaggaaca ctttaaaaag aggaaagaac gcaggcagat gatcttggaa
     4501 aaacggcgca atggaaagtt cgccaagaag atgcagccgg tttataagtg gatgaaccgg
     4561 ctgtctgttc cgcttcagtt tctgcttgca tgtatcatta actttttcat cgaagtcatc
     4621 tcaagacttt cgtttggaga ggcgtgggcg tatcttacaa aaacgccgct tgtgtttttg
     4681 tataattcat gcatgatatt cgttacattt acgatcgttt atctggtgcg aaggcgtgtg
     4741 tttacaagaa tattgctcag cgtgttctgg ctgtttttgg gaatctgcaa cggatatctg
     4801 ctgacgaagc gtgtaacccc ctttaatgca caagatttga aagtcctttc agacgcattg
     4861 gagttgacgg gaaattattt taacacgttt gagcttatta tgatcattat cggtgtgacc
     4921 gctttgatcg tatgggtcgt ttcgatgtgg aaacggggcg gacagtacac aggaaagatg
     4981 caccgctgga tcgcgcttgg cggagttgtc gcttcctttg tcggctgtat ggcgctgaca
     5041 aatctggcga ttgacaaacg tgtcatttct aattacttcg gaaacattgc gtttgcatat
     5101 gaagattatg gatttccgta ttgcttctcg gcaagtttgt ttaatacagg gatcaacgag
     5161 ccgacaggat atggcaaaga gacgatggag aagatcaacg aagaagaaaa actgacgaaa
     5221 agttcgacgg gaagatcgga agaacagcgt ccgaatatta ttttcgtaca gcttgagtcg
     5281 tttttcgatc cgacagaggt agagtggctt cgcttctccg aagatccgat cccgaatttg
     5341 agaaaaatgt tcagcgaata ttcaagcgga tactttaaag tgccgtcagt cggcgcggga
     5401 acggcaaaca cagaatttga agttctcaca ggaatgagta tgcgtttctt cggaccggga
     5461 gaatacccat acaaaacata tgcaaagact cgtacgttag agagcgcggc gtctgccctt
     5521 aaagggcttg gatacggcgc cgaagctctt cataataacg gaggaaattt ctacagccgc
     5581 gcccaggtat ttaacaacat gggatttgat cattacacaa gtaaggagtt tatgaatata
     5641 ctccagacaa cgccgaaagg atgggcgaca gacgatattc tcgttccgaa tattatggag
     5701 tctatggata cgaccgaagg gcaggacttt gtgtttacag tcagcgtaga aggtcacgga
     5761 gagtatccga cagaaaaagt gttggaagat ccggagatcg ttgtaagcgg cgtggaagat
     5821 gaaggcgaaa gaaatgcatg ggaatattat gtaaatcttg tccatgcaat ggatgaattt
     5881 gccggagatc tcgtacgcgc cgtggaggaa agaaacgagc cgactgtact tgtgttctac
     5941 ggcgatcatc ttccgacgat gaatctggaa gcaaaagact taaagagcag atatctgtac
     6001 aatacaaatt atgttatatg ggataacatc gggttagaga aaaaggacgg aaacgtagcg
     6061 gcatatcagg tgatggcaga tgtgtttgaa agactggata tccatgccgg aacagtattc
     6121 aactatcatc agcagcgccg ccatacgaaa aattatctgg cagacctgga actgctgcag
     6181 tacgatatca tgtacggcga tcagtacgtt tacgcagaag aaggctctcc gatgaaagaa
     6241 ggccatatgt atatgggcgt aaaagacgcc gtgatctcac aggtgacaga acaatataat
     6301 aaaacataca gtatttacgg agagaatttt acgaaacaaa gtaaagtatt tattaacggg
     6361 gaaaagcaaa agacgacgtt tttaaataac acccgtctgg atctgaaaga gtcaaaactt
     6421 tccgacggag acacgatcat ggtagcgcag gtcggatcga gcaatacgac gtttcggacg
     6481 acgaagacgt ataagtataa cgccggaacg ctgaccgaga tgccgccgga aaaagatgcg
     6541 ccggaaaacg gcagacaggc gtttgtcgtt gagaaggaag aaaaagaaaa atgacataca
     6601 gtgaagcagt agactatata ctggacattc ccaaatttac aaaaaaaaat gatgcggcac
     6661 acacgaaagc ttttctgaaa acgctcggag atcctcagcg gaaaatgagg atcatccatg
     6721 tggcgggaac aaacggaaaa ggttctgtgt gcgcgtatct ggacggaatg ctccgttcgg
     6781 aacagaaacg aacggggctt tttacgtctc cccatcttgt aaagatcaac gagaggatcg
     6841 ttatagacgg acagatgatt tcggatgaaa gatttctcga agtatttgaa gaaacgatgc
     6901 aggcagtgga caagatgaaa gaagcagggc tttctcatcc tacttttttc gagttccttt
     6961 tcggtatggc ggtgcttgca tttgcagaaa gcggcgtgga atatgccgtt cttgagacag
     7021 gactgggggg aaggcttgac gcgacgaacg ccgtggaatc tccggcggca tcagtgatca
     7081 cttcgatcgg ttttgatcat gaacagtatc tcggagatac gttggagaag atcgcatctg
     7141 aaaaagcggg cattatcaag cctcatgttc ccgtatatta tgcgcagacg acgggagaaa
     7201 gcgattcggt aattgaaaaa gaagcaaaaa agcgcggaag tttctgtaaa aaaataggga
     7261 aagatgcgta cgaaattctt ggaatcgaag acaaacatat tgcattttca tgtttgaatg
     7321 attattatgg aactaccacg tggaaattga acaatatcgg tttgtaccag ccgcataacg
     7381 cattgcttgc gatggaggtg atgcgtggtc tgttcggaga aaacggacat ccgcatttat
     7441 ggaaagaggc tcttgaggcg gtgagatggc gcggaaggat ggaagaagtt ctgcccggag
     7501 tatatgtgga tggagcacat aacataagcg cggtcaaggc gtttgcagag acagtggaaa
     7561 aggccgggtt tgcagatgcg gttttgtttt ccgcggttcg ggataaagac tatgaagaaa
     7621 tgataaaata tctctgtgag catacagagg cggcatgcta tatcgtgacg cagattgaag
     7681 acgcgagggg gacgcgggcg gacgaactgg gagtagtttt tcggaagtac accgacaaac
     7741 ccgtccgtgt gatcggttcg gtgaaggatg cttttgaata tgcggtgagc agcagaagag
     7801 gcggcaatgt atactgtctt gggtcgctgt atctgaccgg aatgatcgag gggtatctcg
     7861 gcagcaggga aaaccagatt cggggaggta gataaaatgc tggattacga agaagaatta
     7921 aaaaaattca agccaagcct tgaagtggat gatattgaaa atgcggttta tcaggaagac
     7981 ttgacggata tgaccgatct gttaaagcag gtaatggatc agaagaagta gaaagataat
     8041 ggtgggtata aatggaatat acgaaaaaga tcgtatatca gtcaaactac tggtataacg
     8101 acggtctgag aaaggcgaag atccgcgata tgtcgggagc gatcatgtct ttgcggaaaa
     8161 gtcttcagtt caacagggag aatatagccg cgagaaatct tctcggtctc gtgtattatg
     8221 ggatcggaga agtgccggaa gctcttgtgg aatggatcat cagcaagaat ttaaagtccc
     8281 gggacaacat tgcggattat tacattaaaa atgttcagtc gtccgccaag gagcttgagc
     8341 agatcgatct tgcgatcaag aaatacaatc agtgtcttac atattgcaga caaaacgggg
     8401 aggatctggc ggcgattcag ttaaaacagg tgatcgcggc acatccgaca tttttaaagg
     8461 cgtatcagct tttaaccttg atctatctgc atacggggca gtattctcag gcgagacaga
     8521 cgatcaagac ggcgagaaaa cttgacacga cgaatgagat cactcttttg tatatgcatg
     8581 agctgacaaa aaaggggaaa aagaaaacaa aagaagagaa aaagaaagaa gaagcggttg
     8641 aatacagtct tggaaacgag acgatcatac agccgaagca ttccagattc agacaagtta
     8701 cggcccagct tgcggttgcc aatatcttta tcggagcggc gatcggagcg gcgatcatct
     8761 ggtttcttgt ggcgcctgcc gtgaatcagt ccagatctga gcggatgaac gatcagatga
     8821 gagaatacgc agacgagatc aagtcgttag aagcgcaggt cagcgctcag acgcggacgc
     8881 ttgacaatta cagagcttca ggcgaagatg cgcaggcgaa tgccgagctg gcacaaaaga
     8941 cagcggaagg ctatgaaaaa ctgctgagtg tggaagggca gttcctttca aatgattatg
     9001 atgacgccgc attggcagat gctcttcttg ggattagcag agacacgttg aaacagacag
     9061 gacaggtcaa gtatgatgag atagcggctg ccgtttatcc cggagcgtgt gaggtgaaat
     9121 tagcagaagg aacacaggcg ctgaattcag gtgattatgc aggggcgata gaccccttgt
     9181 ctaaagttgt gctcatgaac gaaggatata acgacgggca ggcgcttttg aatctggcgc
     9241 aggcttataa aggaagcgga gataacgaaa acgcgacagt ttatttccaa aaagtgatag
     9301 aaaaatacgc gggttcggaa tatgccgcag aagctcagag cggacttgat gagattacaa
     9361 atgaaaataa ttaatacatt acgaaagaaa aggagatgcc gggaggcatg tccttttttt
     9421 gtataggcaa cgagccaaag tccgggcttg cccgagacct tggcgactgc ttacaatcct
     9481 ggaatgtgcc ttttggcact tccggtgatt atatagattt ttgaatttaa gatgccgaaa
     9541 aggagacgac gaagcgatgg aatatcaggt acaggaaaat tgtctgacga tttatcttcc
     9601 ggcagaagtg gatcatcata atgcggagga gatgaagcgt aaggcagata aactaatcga
     9661 tcgaaatcac attaaatacg tgatctttga ttttgaaaat acagatttta tggatagttc
     9721 cggtatcgga gttattatgg ggcgatataa aacgatttct ctgatcggag gagaagtgtg
     9781 ggcggtgcat acaaatgcac gcatccgtaa aatactgact ttgtcagggg tgacaaaaat
     9841 aatgcagatc tatgaggagg aagaagaatg acacctgcaa atgaaatgga attaaagttt
     9901 gacagcaggt cggcgaacga aggctttgcc cgcgtggcgg tagcggcgtt tatgacgcag
     9961 ttgaatccga cggtagagga ggttgcagat gtgaaaaccg ccgtgtcgga agctgtgaca
    10021 aacgtcatta tccacggata tgaaaaagaa gtacgcaaag tatatatcaa atgtatgatt
    10081 gaagaaaaca tatttacagt ggaagtgtgc gatcagggaa aaggaattga gaatatcgag
    10141 gaggcaatgt gtccgatgta tacgaccaga ccggatcagg atcggtcagg tatgggattt
    10201 gcgtttatgg aggcgtttat ggacagcgtg gaagtagagt ctgaactcgg gaaaggaacg
    10261 cgggtgcgga tgaaaaaggc agtcggaaaa ggcagggata tatggacgac acaatcgctt
    10321 tgatcagaaa gtcacacagt ggggataagg cggcaagaga acagcttgta aaagaaaatg
    10381 tcggactggt gcactgtgtc gtgaaacgtt ttcttgaaag gggcgcggaa cgcgaagact
    10441 tgttccagat agggagcatc ggcttgctta aggcgatcga taagtttgat ctgacatata
    10501 atgtaaagtt ttctacttat gcagttccgc ttatttccgg ggaaatcaag cgttttctgc
    10561 gggatgacgg aatgataaaa gtgagtcgtt cgctaaaaga gatcgcttat aaaagcgtac
    10621 aggcaagaga acgtctcatc agttcgctgg ggcgcgatcc gacaatagaa gagttatcag
    10681 aagaagtcgg agtggaaaga gaagaacttg tacaggcgat ggaggcggga tgcgagattg
    10741 aatccattta ccggccggtt catcaaaaag aaggaagcga gatacggctt cttgataaat
    10801 tagaagaaaa agagaaaaga gaagacaaga tcttggatct gatcgttctc aaagaacttc
    10861 tcgagacact ggatgaagaa gaaaaacagt tgatttttct tcggtatttt gctgatcgga
    10921 cacagagcga cgtaggaaag atcatgggaa tttctcaggt acaggtgtcg agatccgaga
    10981 aacggatcat tgaaaaatta agaaaaaaag gaggaagata aaagaatcgt ataaaatccg
    11041 gcaaaggcag catactacag taaaaagaag tggatttact gaaaaatgca gtgaaaagga
    11101 gtcggatcat atgatggaag atggaaacgg acgaaaaaaa gtatggattt gtctgtcgat
    11161 tgtcgtgctt gcggcggttg taatcggagc agtgtattac atgaatatgc cgaaggaaca
    11221 ctcggaagaa ggatttttaa tccggagaga aagcgaggac tgtgcggatg gcatcaggga
    11281 aaacgctcta tattaaaggc gatagagatg tggaggtgac taagccggat gtaacacttg
    11341 cagatctgct ttctatggag tgctccgatc agtcgcttct tccgaagctt aaaacaatga
    11401 aaatattgaa gtttaagaaa acgggcaatc aaagatgtgt gatttcggtg ctgaaaataa
    11461 tcgcatgtat ccacggccaa tatccggata tggaagtgca gaatctggga gaaacagata
    11521 tgatcgtaac atacgaagat cagaaaacac cgtcttatgc atggcatctt acaaaaactg
    11581 cctttgtgac ggctgtgaca ttttgcgggg cggcattttc gatcatggcg tttaataacg
    11641 atgtagatgt aacgaaactg tttgatcaga tttatcatct tgtcaccgga aataagccgg
    11701 gcggcataac gattttggaa atcgcatatt ctgccgggct tatgatcggt attttgatct
    11761 ttttcaatca tttcgggaaa cgccgtttta cagtagaccc tacgccgatg gaagtgcaga
    11821 tgcgggtgta tgaaaacgat attcagacaa cgcttattga aaatgcggca agaaaagaaa
    11881 aagagatcga tgtcggaaaa ggaggcggta tccatgggac aggcggctga ggtgatcgtg
    11941 gaacagcttg tgctggctct tgtcggattg agtgcagggg cggctgtggc aggcggctta
    12001 ttttctttta tcgtggaact cggagtgatc gctgattttg ccgatcggac acacacggga
    12061 gacaaattgc ttttctatga aacatgtgtg gcgcttggcg gaataatcgg aaatatcgtt
    12121 tttctttttt tctcttttgc gccgaatgag gtatcgtttg cttctgcgtc ttttgcgtcc
    12181 ggggcggtac aagttctgtt tgggattttt gccgggattt ttgtcggatg ctggtcgatg
    12241 gcgcttgcgg agatattaaa tgtttttccg atctttatgc gccgcgcgcg aatcgtcaga
    12301 tacgcgtctg tttttgtgat cggcgcagcc ataggaaaag gattgggagc aggattgttt
    12361 ttcttaaaag gctggtagaa gatgaaagac aaggcgggat ttcaacagga aaattatctt
    12421 gaaagaggca gggttttgga ctatcattaa t
//
LOCUS       DBMDXF010000039        12228 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1494_length_12228_cov_2.277480, whole genome shotgun sequence.
ACCESSION   DBMDXF010000039 DBMDXF010000000
VERSION     DBMDXF010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12228)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12228)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12228
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1494_length_12228_cov_2.277480"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(103..1329)
                     /gene="hydF"
                     /locus_tag="ACZPD4_02795"
     CDS             complement(103..1329)
                     /gene="hydF"
                     /locus_tag="ACZPD4_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HIR1378039.1"
                     /translation="MGLNNTPMSERVHIGFFGRRNAGKSSVMNAVTGQDLAVVSDVKG
                     TTTDPVYKSMELLPIGPVVMIDTPGIDDEGELGKLRVKKSYQTLNKTDAAVLVIDAAA
                     GVSGEDKKLLEKIKAKKIPYVVALNKKELAEEEDMERIKKELDSDGGWIIEVSAAAGD
                     GICELKEKIAAAVHQEETENVLVRDLLHPSDIVVLVVPVDSAAPKGRLILPQQQVIRD
                     ILEAEAVSVVVKENGLKNILDQLKENPRMVITDSQVFKKVSKDTPADVPLTSFSILMA
                     RYKGNLEQAVRGVTELDRLKDGDVVLISEGCTHHRQCEDIGTVKIPRWIREYTGKEIQ
                     IETTSGTEFPDDLSKYKLIIHCGGCMLNEREMKYRYSCAADQGIPITNYGIMIAYVNG
                     ILKRSVELFPELYEIL"
     gene            complement(1382..2605)
                     /gene="tyrS"
                     /locus_tag="ACZPD4_02800"
     CDS             complement(1382..2605)
                     /gene="tyrS"
                     /locus_tag="ACZPD4_02800"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1378040.1"
                     /translation="MGIYEELQARGLIAQVTDEERIRDLVNSGKAVFYIGFDPTADSL
                     HVGHFMALCLMKRLQEAGNKPIALIGGGTGYIGDPSGRTDMRSMMTPEQICHNCDCFK
                     EQMSKFIDFSEGKALMVNNADWLLDLNYVEVLREIGPHFSVNRMLTAECYKQRMEKGL
                     SFLEFNYMIMQAYDFYALFQKYGCNMQFGGDDQWSNMLAGTELIRRKLGEDAGAMTIT
                     LLLNSEGKKMGKTQSGAVWLDPNKTSPFDFYQYWRNVADADVLKCIRMLTFLPLEEID
                     KMDSWEGAQLNTAKEILAYELTKLVHGEEEAKKAQESARALFSKGNAAEMPTAQLTEE
                     DLTDGKIDILTMLVKSGLVTSKSEARRAVQQGGVAVDGEKVSDIAASFGKEELSGEGI
                     ILKKGKKNFRRVVAE"
     misc_binding    complement(2763..3046)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3121..3915)
                     /locus_tag="ACZPD4_02805"
     CDS             complement(3121..3915)
                     /locus_tag="ACZPD4_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIR1378041.1"
                     /translation="MSYLLEVCADSVRSAVEAQAGGADRIELCSGLVIGGLSPSVAMF
                     EQVKKNVDIPVRVLLRPRFGDFCYDGYEFETLKEEVCLFREAGADGVVIGILKPDGRL
                     DTDRMKELVECAKSESKSGKRPCAVTFHRAFDVCRSPYEALRQCIELGIDTILTSGQK
                     DSAWNGRELLKELVREAAGEIEILAGAGISPEVIGKLADYAGVTSFHMSGKKVTDSKM
                     EFRREDVPMGIPGFPEFDIWQTDAGLVGNAKRILIDLQEESLKNDR"
     gene            complement(3928..4659)
                     /gene="nagB"
                     /locus_tag="ACZPD4_02810"
     CDS             complement(3928..4659)
                     /gene="nagB"
                     /locus_tag="ACZPD4_02810"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1378042.1"
                     /translation="MKIYETENYEEMSRKAANILSAQVILKPDCVLGLATGSTPIGTY
                     DKLVEWYEKGDLDFSEVKTVNLDEYRGLTRDNDQSYYYFMHEHLFDRVNIDPENTNVP
                     NGTEPDADKECERYEKLIESMGGVDIQLLGLGHNGHIGFNEPDSAFAQTTHCVDLTES
                     TIEANKRFFASADDVPRQAYTMGIGTIMRAKKILLIVNGEAKADIVAKAFFGPVTPEV
                     PASILQLHNDVVIVGDKAALSKIPR"
     gene            complement(4765..5580)
                     /gene="truA"
                     /locus_tag="ACZPD4_02815"
     CDS             complement(4765..5580)
                     /gene="truA"
                     /locus_tag="ACZPD4_02815"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1378043.1"
                     /translation="MRRIKLTVAYDGTDYCGWQIQPNGITVEEVLNKALSDLTKETVK
                     VIGASRTDAGVHARGNVAVFDTKSPIPPERIAYAVNALLPDDIVVVRSEEVGCDWHPR
                     YQNSIKTYEYTIVNREMPDPLRRRDSWFVSFPLDIDDMKKAARYFIGEHDFKTFCSVH
                     TGVKTTVRRIDSLDVEKKGERIKIRISGNGFLYNMVRIIAGTLVEVGRGAFAPSEVKE
                     MLEQKERGRAGATAPPQGLVLLGIVYDGQADTERGQIQKDTDSSRMREDADGR"
     gene            complement(5801..6625)
                     /locus_tag="ACZPD4_02820"
     CDS             complement(5801..6625)
                     /locus_tag="ACZPD4_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1378044.1"
                     /translation="MIRDITIGQYYPVKSRIHSLDPRVKIICTLLFLVSLFVQSSFLG
                     YVIATLFLGMVIYMSKVPLKYIVKGLKPIVILLMFTVVMNLFLTRGGETLLHFWIFTI
                     TEEGVRTSVFMALRLMYLVAGSSLMTFTTSPNGLMDGMEKLLNPLNKLHLPVHEVAMI
                     MSIALRFIPILLEETDKIMKAQIARGADFESGNIIQRAKAMIPILVPLFVSAFRRAND
                     LAMAMEARCYHGGEGRTRMKPLKYNGRDKAAYLITILYAAGVFAVGKFVPFQLWIF"
     gene            complement(6622..7467)
                     /locus_tag="ACZPD4_02825"
     CDS             complement(6622..7467)
                     /locus_tag="ACZPD4_02825"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1378045.1"
                     /translation="MSIALEHVNYIYSPGTAYEKRALNDISLEIGQGQFVGIIGHTGS
                     GKSTLIQHLNGLMKATSGDILYDGQSIYAEGYDMRKLRSQVGLVFQYPEHQLFEVDVI
                     SDVCFGPKNQGLSEEESEKNAREALELVGFPEKYYKQSPFELSGGQKRRVAIAGVLAM
                     KPKVLILDEPTAGLDPKGRDEILDQIAKLHKETGMTVVLVSHSMEDVARYVDRIIVMN
                     KGEKMLDSTPKEVFRHYKELEEVGLAAPQVTYVMHDLKDRGFDVSPDATTIEEAADEI
                     MRSFI"
     gene            complement(7452..8303)
                     /locus_tag="ACZPD4_02830"
     CDS             complement(7452..8303)
                     /locus_tag="ACZPD4_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1378046.1"
                     /translation="MNMIHAEKLVYEYDKRDEEGNVIGTNRAIDGVDIDVPQGSFVAV
                     LGHNGSGKSTLAKHMNAILIPTGGTVWVDGQDTKDPENLWDVRKSAGMVFQNPDNQII
                     GTVVEEDVGFGPENIGIPTDEIWERVGESLRLVGMTEYRASSPNKLSGGQKQRVAIAG
                     VIAMEPKCIVLDEPTAMLDPNGRKEVLQAVARLRKEKNVTVILITHYMEEVIDADQVF
                     VMDSGHIVMHGTPREIFGRADELKSYRMDVPQVTMLAEELRKRGLDIPKGILRREELV
                     EALCRLR"
     gene            complement(8470..9351)
                     /locus_tag="ACZPD4_02835"
     CDS             complement(8470..9351)
                     /locus_tag="ACZPD4_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1378047.1"
                     /translation="MWKKTKEMKWKTAKLLWGDLFYDIAGAVLFAAGVYTFAENADFA
                     PGGITGISLLLNHVFGIPVGTLTLFINLPLIVISYRTLGKRFLLKTAKSMVITTFFLD
                     VVFPVFPMYEGQRMMAALCSGALMGAGMVMFYMRGSSSGGLDFVIMPIKKKRPHLSVG
                     KITMIIDLGIILLGWPVFGDVDAALYGLVSTFASVVVIDKVLYGLGAGTLAFVVTEKG
                     DEVAAKIAELTKRGVTSVKATGTYTGKKKNVLLCACSKSEAFLVKCAAEEADENMFLM
                     FMETSEVFGEGFRSSSD"
     gene            complement(9429..10466)
                     /locus_tag="ACZPD4_02840"
     CDS             complement(9429..10466)
                     /locus_tag="ACZPD4_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR1378048.1"
                     /translation="MSVKRDYYEVLGLSKGADAGSIKKAYRKLAKKYHPDTNQGDKQA
                     EKKFKEVTEAYTVLSDPEKKKMYDQFGHAAFDQGASADPFGKDTSGNPFSGGSYHRAY
                     TNGNGGYQEYHFESGNMDDMFGDMFGDIFGRGMHGSKGEEDFRGFHGFRGYERQRDAK
                     GEDLHAEIAVTFEEAVFGCDKTFTFKDPNSQGVSKSLQVHIPAGIDTGKSIRLRGKGM
                     PGVNGGENGDLFLKVNVGVKAGYERKGSDVYTTVFVPYTTAVFGGETKVQTLYGDVLC
                     KIAKGTQSGTKIRLKGKGIVSMKNPEVRGDQYVTVQIAVPKYLNPEAEQKLREFENVC
                     AGRSSERRKSA"
     gene            complement(10739..11932)
                     /gene="tuf"
                     /locus_tag="ACZPD4_02845"
     CDS             complement(10739..11932)
                     /gene="tuf"
                     /locus_tag="ACZPD4_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR1378049.1"
                     /translation="MAKAKFERNKPHCNIGTIGHVDHGKTTLTAAITKVLSHRVEGNA
                     SVDFENIDKAPEERERGITISTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGVMAQTKEHILLSRQVNVPYIVVFMNKCDMVDDEELLELVEMEIREVLS
                     EYDFPGDDTPIIQGSALKALEDPDGEWGDKIMELMDAVDTWIPTPERATDKPFLMPVE
                     DVFSITGRGTVATGRVERGTLHVSDEVEIIGIHDDVKKTVVTGIEMFRKLLDEAQAGD
                     NIGALLRGIQRTEIERGQVLIKPGTVNCHKKFTCQVYVLTKDEGGRHTPFFNNYRPQF
                     YFRTTDVTGVCELPAGIEMCMPGDNVEMTVELIHPVAMEEGLRFAIREGGRTVGSGTV
                     AKIIE"
     gene            complement(12056..>12228)
                     /locus_tag="ACZPD4_02850"
     CDS             complement(12056..>12228)
                     /locus_tag="ACZPD4_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378050.1"
                     /translation="GGGKMIRGFVPLSEMFGYATDLRSRTQGRGNYSMFFEKYEPVPK
                     SVQEKVLSNKNA"
BASE COUNT         3059 a         3466 c         2089 g         3614 t
ORIGIN      
        1 agcctgcatt accagtgaca ttcccaatgt ccctctttaa ttaaattcat tataacaaca
       61 acatataata ttgtcaacca aaacaattcc tgttttacag ttttacagta tttcataaag
      121 ctcgggaaac aattctacgc ttcgctttaa aataccgttc acataggcga tcataattcc
      181 gtaattcgtt atcggaatcc cctgatccgc cgcgcagcta taacggtact tcatctcccg
      241 ctcattcagc atacagccgc cgcagtgaat gatcaactta tatttactta agtcatccgg
      301 aaattccgtt cccgatgttg tctctatctg aatctctttg cctgtatatt ccctgatcca
      361 gcggggaatc ttcaccgttc cgatatcctc gcactgtcgg tggtgcgtac atccttcgct
      421 gatcagcacg acatctccgt cttttaatcg atccagttct gtcacacccc taactgcctg
      481 ttccagattg cctttatacc gcgccatcaa tatcgaaaaa gaagtaagcg gcacatcggc
      541 aggagtatcc tttgacacct ttttaaagac ctgactgtcc gtaatcacca ttctcggatt
      601 ttcttttaac tgatcaagaa tatttttcag tccgttttct tttacaacta ccgataccgc
      661 ctctgcttca agaatatcac ggatcacctg ctgctgagga aggatcagtc tgcctttcgg
      721 agctgccgaa tcgacaggca ctacaagaac gacgatatca gaaggatgaa gcagatctcg
      781 aacaagaaca ttctccgtct cctcctgatg cactgctgcc gcaatcttct ctttcagctc
      841 acaaataccg tcgcctgccg ccgcgctcac ttcgatgatc cagccaccat ccgaatcaag
      901 ttctttcttg atccgttcca tatcctcttc ttctgcaagt tcttttttat tcagcgcgac
      961 aacataagga attttctttg ccttgatctt ttcaaggagc tttttgtctt ccccggaaac
     1021 tcccgctgcc gcatcgataa caagtacagc cgcgtctgtc ttattaagcg tctggtaact
     1081 ttttttcacc cgcagtttcc caagttctcc ttcatcgtct attcccggtg tatctatcat
     1141 cacaaccgga ccgatcggaa gcagctccat tgacttatat accggatccg tcgtcgtacc
     1201 tttcacatct gatacaactg ccagatcctg ccctgtaacg gcattcataa cactcgactt
     1261 tccggcattc ctccttccga aaaatccaat atgaacccgt tcagacatcg gtgtgttatt
     1321 caatcccata ccgccacttc ctttccttct ttgatctgat ccgttctttt ttcgccgctt
     1381 cttactctgc aacaacccgg cggaagtttt tctttccttt tttcaaaata attccttcgc
     1441 ccgagagttc ctcttttccg aaagatgcgg caatatcaga aactttttca ccgtctacgg
     1501 caacgcctcc ctgctgtacc gcgcgtcttg cctcggattt agatgtaaca agtccgctct
     1561 tcacaagcat tgtaagaata tcgatctttc cgtctgtaag atcttcttct gtaagctgtg
     1621 cagtcggcat ctccgccgca ttacctttac taaacaaagc tcttgcgctt tcctgcgctt
     1681 tcttcgcttc ctcttctcca tggacaagtt ttgtcagctc atatgccaga atctctttcg
     1741 cagtattaag ctgcgctcct tcccacgaat ccatcttatc tatttcctca agcggaagga
     1801 acgtaagcat acgaatacat ttcaaaacgt ctgcatccgc aacatttctc caatactgat
     1861 aaaaatcaaa cggcgatgtt ttatttgggt caagccatac agcacctgac tgtgtttttc
     1921 ccatcttctt tccttcggaa ttcaaaagca gcgtaatagt catcgcgcct gcatcttctc
     1981 ccaattttct gcggataagc tctgttcccg caagcatgtt gctccactga tcgtcgccgc
     2041 cgaattgcat attacatccg tatttctgga acaatgcata gaaatcataa gcctgcatga
     2101 tcatgtagtt aaactcaagg aaacttaatc ctttttccat cctctgctta taacactctg
     2161 ccgtaagcat acggtttacc gaaaaatgcg gtccgatctc acgaagcact tctacataat
     2221 tcagatcaag aagccagtcc gcattattta ccataagcgc ttttccttct gagaaatcaa
     2281 taaacttgct catctgctct ttaaagcaat cgcagttatg acagatctgc tccggcgtca
     2341 tcatgcttct catatctgtt cttcctgacg gatctccgat atatcccgtt ccgcctccga
     2401 tcaatgcgat cggcttattt cccgcttcct gtaaacgttt cataagacac agcgccataa
     2461 aatgtcctac atgaagacta tctgccgtag gatcaaatcc aatataaaat acagcctttc
     2521 cactgtttac taaatcgcgg attcgttctt catccgtcac ctgcgcaatc aaacctcttg
     2581 cctgaagttc ctcgtaaatt cccattttct ttctccttat tctctgtaaa atatcaaatg
     2641 tttctaaatc accggaagtg ccaaaaggca cattccggga ttgtaagcag tcgccaaggt
     2701 ctcgggcaag cccggacttt ggctcgttgc ctatacaaaa aaaccgccct tatcgctcac
     2761 gcaataagga cggatatttc cgtggtacca ccttaattca ccgaatcgcc gaacatcggc
     2821 gctttcagtc tcattccggt acgataacgt ataccactcc gtcacagcct actcatcatc
     2881 tgccatttta catccgcggt aatggtttct cctgtaccgc aggacagact tcttttcagt
     2941 gcgaagctcc gggatgtatt cacagattcg tctcacgcgc ctctcatcaa ccggctgctt
     3001 tctgtcatca cgttcacctg ttacttcttc ccttcaaagc ctatataata tgaagcatat
     3061 cttagcttac acttcttctt ttgtcaagag ccgactacat attgatcagc cacaaattct
     3121 tcaccggtca ttttttaacg actcttcttg taaatcaatc aatatccgct ttgcattccc
     3181 gaccagcccg gcatccgtct gccaaatgtc aaattcagga aaacccggta ttcccatcgg
     3241 cacgtcttct ctgcgaaact ccatcttact gtcagtcact tttttgcccg acatatgaaa
     3301 agacgtcacg cctgcatagt ctgccagttt tccgattact tcaggactta tcccggctcc
     3361 tgcaagaatc tcaatctccc cggcagcttc cctcacaagt tcttttaaaa gttccctgcc
     3421 attccatgcg ctgtctttct gcccgctcgt aagaattgta tcaattccca gctctataca
     3481 ctgcctcagc gcttcatacg gactccggca cacatcaaaa gctctgtgaa aagtgaccgc
     3541 acatgggcgt ttcccacttt tactttcgga ctttgcacat tcgacaagtt ccttcatcct
     3601 atctgtatca agccttccgt ccggttttaa aataccgata acaacaccgt cggctcccgc
     3661 ctctctaaac agacagactt cttccttcag cgtctcgaat tcatatccgt cataacagaa
     3721 atctccgaat ctcggacgaa gcagcactct tacaggaata tcgacatttt tctttacctg
     3781 ttcaaacatc gcgaccgacg gagacagacc tccgatcaca agacccgaac acaattctat
     3841 gcgatccgct ccgcccgcct gcgcctcaac cgcagatctt acactgtcgg cacatacttc
     3901 aagtaaatac gacatcgccg cgcagcctta tctcggaatc ttcgacagag ccgctttgtc
     3961 tccgacgatc acaacatcgt tatgaagctg aagaatcgat gccggaactt ccggagttac
     4021 cggtccgaaa aatgcctttg caacgatgtc ggcttttgcc tctccgttta caataagcaa
     4081 gattttcttc gcacgcatga tcgttccgat acccatcgta tacgcctgac gcggcacgtc
     4141 atcggcagat gcgaaaaatc tcttattcgc ttcaatcgta ctctccgtca gatcaacaca
     4201 gtgtgttgtc tgtgcaaaag cgctgtccgg ctcgttaaat ccgatatgtc cgttgtgtcc
     4261 cagaccgaga agctgaatat caacgcctcc cattgattcg atcagttttt cgtatctttc
     4321 acattctttg tcggcatccg gctctgtacc gttcggaaca tttgtattct caggatcgat
     4381 attcacacgg tcaaacaaat gctcatgcat aaaatagtag tagctctgat cgttatcacg
     4441 agtcagacct ctgtattcat ccagattcac tgtctttact tccgagaaat caagatcacc
     4501 tttttcatac cactctacaa gtttatcgta tgttccgatc ggtgtagatc ccgttgcaag
     4561 accgagtaca caatccggtt tcaaaattac ctgtgcggaa agaatatttg ccgcttttct
     4621 actcatttct tcataattct ctgtttcata aattttcata tctttcgccc tcctatcgct
     4681 acatttaagt ctaacaatct gccttataaa agtcaagcag aaagaacgct cttcctacct
     4741 gtctatgcct gtatctttct gtatctatct gccgtccgca tcttcccgca tccggctgct
     4801 gtctgtatct ttctgtatct gtcctctttc tgtatcagcc tgcccgtcat acacgattcc
     4861 taaaaggaca agaccttgcg gcggcgcagt agctcccgct ctgcctcttt ctttttgctc
     4921 aagcatctct tttacttctg aaggagcaaa tgcaccccgt ccgacttcta caagcgttcc
     4981 cgcaatgatc cgaaccatat tatacaaaaa cccgtttccg ctgatgcgta tttttattcg
     5041 ttctcccttt ttttcaacat ccagactgtc gatcctgcgc accgttgttt ttacaccggt
     5101 atgaacgctg caaaatgttt tgaaatcatg ttcgccgata aaatacctcg ccgccttttt
     5161 catatcatca atatccagcg gaaaagaaac gaaccacgag tctcttcttc tgagcggatc
     5221 cggcatttca cggtttacta ttgtatattc atacgttttt atcgagtttt gatatctggg
     5281 gtgccagtca catccgactt cttccgaccg cacaactacg atatcatcgg gaagaagcgc
     5341 gttgactgca tacgcaatcc tctccggcgg aatcggcgac tttgtatcaa agacagccac
     5401 attcccccgc gcatgaacgc ctgcgtccgt tcggcttgca ccgatcacct tcacagtttc
     5461 ttttgtcaga tcggacagcg ctttatttaa aacttcctct accgtaatcc cgtttggctg
     5521 tatctgccac ccgcaataat ctgttccgtc gtaggcgaca gttaatttaa tccgtctcat
     5581 atttttattc gttccccttt attctgtcag atcatatgcg ttttcaactt tccaccgtgt
     5641 cttttccttc ttcgccgtct atccccggaa aagcagacct gcacagacct gtcatgccaa
     5701 atactttttc agcctccgca tcgggctgcc tttggagaat ccacaactga aaccgaagtc
     5761 cgccagatac tctgtattct cctccgtcac caggctgctt ttagaaaatc cacaactgaa
     5821 acggcacgaa ttttccgacc gcaaacactc ccgctgcata gagaatcgta atcagatacg
     5881 ccgctttgtc tcttccgtta tatttcagcg gcttcatcct cgtccttccc tctcctccgt
     5941 gatagcagcg cgcttccatc gccattgcaa gatcgtttgc ccggcggaaa gcagaaacaa
     6001 acaacggtac aagaatcggg atcatcgcct ttgccctctg aataatattc ccgctctcaa
     6061 aatctgctcc tctggcgatc tgcgctttca tgatcttatc cgtctcctcc aaaagaatcg
     6121 gaataaagcg aagcgcgatc gacatgatca tcgcaacctc atgaaccgga agatgaagct
     6181 tgttcaaagg attcagcagt ttttccattc cgtccatcaa tccgttggga gatgtcgtaa
     6241 acgtcataag agacgatccc gccacaagat acattaagcg aagtgccata aacaccgaag
     6301 tacgaacgcc ttcttccgta atcgtaaaaa tccaaaaatg cagcagcgtt tcgccgcctc
     6361 ttgtcaaaaa cagattcata acgaccgtaa acatcagaag gatcacgatc ggttttaacc
     6421 cttttacaat atatttcaga gggactttcg acatatagat taccattccg agaaacaatg
     6481 tcgcaattac atacccaagg aaagaactct gaacaaacaa agagacaaga aataaaagag
     6541 tgcagataat cttcacccgt gggtctaaac tatgtatccg gctttttacc gggtaatact
     6601 gcccgatcgt aatatctctg atcatataaa actcctcatt atctcatccg cagcttcttc
     6661 aatcgttgtc gcatcaggcg acacatcaaa tccgcggtct ttcaagtcat gcatcacata
     6721 agtaacctga ggtgccgcaa gtccgacctc ctccaactct ttataatgcc ggaacacttc
     6781 tttcggagta ctatccagca tcttctcccc cttattcatc acaatgatac ggtctacata
     6841 ccgcgccaca tcttccatac tgtgcgacac aagaacgacc gtcatccctg tctctttatg
     6901 cagttttgca atctggtcaa gaatctcgtc tctccctttc ggatcaagtc ccgccgtcgg
     6961 ctcatccaaa ataagcactt tcggcttcat agccaacact cccgcaatcg ccacacgcct
     7021 cttctgtcct cccgaaagct caaacggaga ctgcttataa tacttttcgg gaaatccgac
     7081 taattccaga gcttccctcg catttttctc actctcttcc tccgacaatc cctgattctt
     7141 cgggccgaaa caaacatcac ttatcacatc gacttcaaac aactgatgct caggatactg
     7201 aaacacaaga cctacctgac tgcgcagttt tctcatgtcg tacccttccg cataaatgct
     7261 ttgtccgtcg tacagaatat ccccggaagt cgccttcata agtccgttta aatgctgaat
     7321 cagcgtagat tttcctgatc cggtatgacc gatgatcccg acaaactgcc cctgcccgat
     7381 ctccaaacta atgtcattga gcgcacgttt ctcataagcc gtgccgggac tgtatatata
     7441 atttacatgc tctaacgcaa tcgacataac gcttccacca actcttccct tcttagaatt
     7501 cctttcggaa tatcaagacc acgttttcta agttcctccg caagcatcgt aacttgcgga
     7561 acatccatcc tgtatgattt cagctcatct gcacggccga aaatttctct cggcgtcccg
     7621 tgcattacaa tatgaccgct gtccataaca aacacctgat ccgcatcgat cacttcttcc
     7681 atataatgag tgataagaat gaccgttacg ttcttctctt ttctcaaacg cgcaaccgcc
     7741 tgaagcacct ccttgcgccc gttcggatca agcatggcag tcggttcatc caaaacaata
     7801 cacttcggct ccatcgcgat cactcctgcg atcgccacac gctgcttctg accgccggaa
     7861 agtttattcg gagaggacgc ccgatattcc gtcatcccga caagacgcag actttcccct
     7921 actctttccc atatctcatc tgtcggtatc cctatatttt caggtccgaa accaacatct
     7981 tcttctacga cagttccgat gatctggttg tcaggatttt gaaataccat tcccgcgctc
     8041 tttcgcacgt cccacagatt ctccggatct ttcgtatcct gtccgtccac ccatacagta
     8101 ccgcctgtcg ggatcaatat tgcattcata tgctttgcaa gagtagactt tcccgaaccg
     8161 ttatgaccga gaaccgccac aaaagaacct tgcggcacat caatatcaac cccgtcaatg
     8221 gcgcggtttg ttccgatcac atttccttct tcatcacgtt tatcatattc atatacaagc
     8281 ttttccgcat gtatcatatt cataggcaga ccgcctttcc cggttttatt tcaccttaat
     8341 tcttctcaat ctctccgcac ttacaaaaac tgcgttttca gacagattat ggattccatt
     8401 ttactgcatg aaaatgtttt tttcaacccg ccacaaacgc attttttctc cctgccagaa
     8461 acaaacgctt caatccgaac tgctccgaaa cccttctcca aacacttcac tcgtctccat
     8521 aaacattaaa aacatattct catccgcttc ttccgccgca catttcacga gaaatgcctc
     8581 agatttactg catgcacaaa gcaagacatt ctttttcttt cccgtgtatg ttcccgtcgc
     8641 tttgacagac gttacccctc tctttgtcag ctctgcgatc tttgccgcca cttcatctcc
     8701 cttttcggtt acaacaaagg ccagcgttcc tgctccgagt ccatacaaca ctttgtcaat
     8761 cacaacaacc gatgcaaatg tagagaccaa tccataaagg gccgcatcta catctccgaa
     8821 tacaggccat cccagaagaa tgatcccaag atcaatgatc atcgtaattt tcccgacaga
     8881 tagatgagga cgttttttct tgatcggcat gatgacaaaa tcaagacctc ccgaagaaga
     8941 gccccgcata tagaacataa ccatcccggc tcccatcagc gctccggaac aaagcgcagc
     9001 catcatcctc tgtccttcat acatcggaaa caccggaaaa acgacatcaa gaaaaaatgt
     9061 tgtgatcacc attgatttcg ccgttttcaa aagaaaacgt ttccccagtg tacgataact
     9121 gatcacgata aggggcagat ttataaaaag agtaagtgtt ccgaccggaa tcccgaaaac
     9181 atgattcagc aatagagata ttcccgtaat tccgccggga gcaaaatcgg cgttttccgc
     9241 aaacgtatat acccctgccg caaacaatac cgcccccgca atatcataaa ataaatcgcc
     9301 ccacaataat tttgcagttt tccatttcat ttcctttgtt tttttccaca taaaaacctc
     9361 tgtatgccat acttattcac aatgcttagt tacactgtat tcagtatatg caaaacagag
     9421 gtttctaatc atgcgctttt tctgcgttcc gagcttcttc ccgcgcatac attttcaaat
     9481 tcacgaagct tttgctcagc ttcgggattc agatatttcg gtactgcaat ctggactgtt
     9541 acgtattgat cgcctctcac ttccggattt ttcatcgaga cgattccttt tcctttcagg
     9601 cggatcttcg tccccgactg cgttcccttt gcgatcttac ataaaacatc cccgtacagc
     9661 gtctgcactt tcgtttctcc gccaaacact gccgtcgtat acggaacaaa aactgtcgta
     9721 taaacatcgc tgcctttccg ttcgtagcct gcttttactc ccacatttac ttttaaaaac
     9781 agatctccgt tctctccgcc gttcacccct ggcattcctt ttccgcgcag acggatactc
     9841 ttacccgtat caatccccgc aggaatatga acctgcaaac tcttagaaac gccctgacta
     9901 ttcggatctt taaaggtaaa cgttttatca catccaaaga cagcttcctc aaaagttacc
     9961 gctatctccg cgtgcaggtc ttctcctttt gcatccctct gccgctcata tccgcgaaat
    10021 ccatgaaatc cgcgaaaatc ctcttcccct ttgcttccat gcattccgcg gccgaaaata
    10081 tctccaaaca tatctccgaa catatcatcc atatttccgc tttcaaaatg gtactcctga
    10141 tatccgccgt ttccgttcgt ataagctctg tgataagaac ctcccgaaaa cggattgcct
    10201 gatgtatctt tgccaaacgg atcagccgat gctccctgat caaatgccgc atggccaaac
    10261 tgatcgtaca tctttttctt ctccgggtcg ctcagtaccg tatacgcctc cgtcacttct
    10321 ttaaacttct tctccgcctg tttatcgcct tgattcgtat caggatgata tttcttcgcc
    10381 agctttctgt aagccttttt tatcgagccg gcatctgctc cttttgatag accgagcact
    10441 tcataataat ctctttttac tgacattttc cttccctccc acaattacaa attcaagatc
    10501 actgatttta ttcttgttcc attctactaa aaatcattat ttacacaaat tatctctttg
    10561 atataaaacc accccggaga aatttccggg gtatctttag ttctgttcta tttgtaatat
    10621 agcagttgtc tttttaaatg tcaatatttt cgtaaatatt tttccgaaat tacaaaaacc
    10681 cgcagagcaa acactctgcg ggtcatatcg ctatatcaaa tctatattca gattatgatt
    10741 actcgatgat cttagctaca gtacctgatc ctactgtacg tccaccctca cggatagcga
    10801 aacgaagacc ttcttccata gctaccggat gaatcagttc aactgtcatc tctacgttat
    10861 ctccaggcat acacatctcg ataccagccg gaagttcgca aacacctgtt acgtctgttg
    10921 ttctgaagta gaactgtgga cggtagttgt tgaagaatgg agtatgacgt ccaccttcgt
    10981 cttttgtcag tacgtaaacc tggcatgtga atttcttatg gcagtttact gtacctggtt
    11041 tgataagaac ctgtcctctt tcgatctctg ttctctggat accacgaaga agtgctccga
    11101 tgttatctcc agcctgagcc tcatcaagaa gcttacggaa catctcgatt cctgttacaa
    11161 cagtcttctt aacatcatca tggataccga tgatttcaac ttcgtcagat acgtgaagtg
    11221 ttccacgctc tactctacca gttgcaactg ttccacgtcc tgtgatagag aatacgtcct
    11281 ctactggcat caggaatggc ttatctgtag cacgctctgg tgttgggatc catgtgtcaa
    11341 cagcgtccat gagttccatg atcttgtctc cccactctcc gtctggatct tcaagagcct
    11401 taagagctga tccctggatg atcggtgtgt catctcctgg gaagtcatac tcgctaagta
    11461 cttcacggat ctccatctct acgagttcaa gaagctcttc atcgtcaacc atatcacatt
    11521 tgttcatgaa tacaacgatg tatggtacgt taacctgacg ggacagaagg atatgctctt
    11581 tagtctgagc cataactccg tcagtagcag ctacaacaag gatagctccg tccatctgag
    11641 cagctcctgt gatcatgttc ttaacgtagt cagcatgtcc tgggcagtca acgtgtgcgt
    11701 agtgacggtt agctgtctca tactcaacgt gagctgtaga aattgtgata ccacgctctc
    11761 tctcttctgg agctttatcg atgttctcga aatctacaga tgcgttacct tctactctgt
    11821 gagaaagtac ttttgtgata gcagctgtaa gagttgtctt accgtggtca acgtgaccga
    11881 tagtaccgat attacaatgc ggtttgtttc tttcaaattt agcctttgcc attttgaata
    11941 tcctccttaa aaatatagcc cgatatgggg caacctagtt ttggtttcga ttttaaacct
    12001 ggctaagttt gattatattt caaacttagc cgattttcaa gccttttata atattttagg
    12061 catttttatt ggataatacc ttctcctgta cagacttcgg tactggctcg tatttctcga
    12121 agaacattga gtagttacca cgtccctgag ttctggaacg aaggtctgtt gcatatccga
    12181 acatttcgga aagcggaacg aatccacgga tcatcttgcc tccgccga
//
LOCUS       DBMDXF010000040        12144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1504_length_12144_cov_2.070746, whole genome shotgun sequence.
ACCESSION   DBMDXF010000040 DBMDXF010000000
VERSION     DBMDXF010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1504_length_12144_cov_2.070746"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            31..381
                     /locus_tag="ACZPD4_02855"
     CDS             31..381
                     /locus_tag="ACZPD4_02855"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR1378051.1"
                     /translation="MDNKKTDQYYIDKIITDLTFIRTHTESLSKLKLEEDEILVDSIM
                     FRLIQVAENSEKLTEKFKETYPSIPWRAIKGLRNRIVHEYGNVDLTIVYDTVKKDIPE
                     LIGILEGICRCKKE"
     gene            complement(437..1873)
                     /locus_tag="ACZPD4_02860"
     CDS             complement(437..1873)
                     /locus_tag="ACZPD4_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378052.1"
                     /translation="MPDFATLLSEHIHNKNIKTYALAQYCNLDRSNMYKIIKGQRKPS
                     SLEMVEKMCKFMHLSPSEQADMEESYHIALIGRENYYRRKAVQQFFEEFRIPTLQLPA
                     TSPSNASDYADNATLLHSHLEINRSLLYIIASELKKSGGKISLLIQPDYTFLMNILTA
                     GDYASSSVDIDHIICLNNNQNVSFARQNYNLNCLRNILPLYSNHYQYNCYYYYDDIDA
                     VTSAFALFPYAVITTEYACLISSDMQSGFITKDPESLKLFSYLFSQYLAQTTPLLRPV
                     TNLGGQIQYVENTMQNITEGYFFQMLPCLTRFLTRDMLETYIVKDLPHRSELLDRLQN
                     YLHELQSIPASADLTFICSIEGIRKFLNTGRVGEYPAYAYTPLNLSDRLFLIRQLANS
                     MLNEHNRLLKRAIGMIDHEFYLFAGRQYGYFMFQTSNTDRMIYLNIEEPGLLFTFFDF
                     CDTLDDEIFFTSEEAYRKIHDLIQEYSK"
     gene            2125..3813
                     /locus_tag="ACZPD4_02865"
     CDS             2125..3813
                     /locus_tag="ACZPD4_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR1378053.1"
                     /translation="MGKKLTKKLLVFLTLMCMIIGMNVSVSAETGSTGNTKAISAAEN
                     PADGTLQVWLEYEDDNGNSVALSGGSCFLINEEYVLSNYHIFNLNEEIAPGVTIRSAA
                     METLGLSELKDNDPHLKIRVYANRDMSVLATIHDSVQSQTMDYVAMKLSDKIYDRKPL
                     ALGDSSTVQQTQTVNAYGFPEDSIENKEFNTAADVSATDGTISKLTVGGRADFFEHTA
                     QLNHGNSGGPLVDSNKSVIGVNTFTYGNSSDQKYYAVQINAIKSGLDTFGISYINAGA
                     QATTQTPDEEEPEEKEGPDADLVTALKSEITKAKAVDTKNYTEESVKVLNDTISEAEK
                     VANDTEATDAQVTSATSDVKAAVENLEEKSGMNFLLIGGIAAGVIVVVVVIIIIVVSS
                     NSKKKKKAQAGNGNVPPSGMPQQPNGAVVPPNRAPEQPKAPYAQPYQAEPDGAGETTL
                     LDSGAGETTLLNGAGSAYLIRKKNGEKITINSQNFAIGKERRRVNYCISDNTSVSRYH
                     VVITKKGSDYYAADQKSSNFTFVNGVQLNPYQETLLTDRSTLKISDEEFEFHAS"
     gene            3874..4659
                     /locus_tag="ACZPD4_02870"
     CDS             3874..4659
                     /locus_tag="ACZPD4_02870"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR1378054.1"
                     /translation="MGFLSVVHSDRGIKKNTNQDSVLIKEASTDYGDVMLAVVCDGMG
                     GLAKGEVASAALIKAFSMWFEVQFPELLYRRRTADGINRMELENEINKLILDVNQRIA
                     DHGKISHIAMGTTAAVLLMAEGKYYTMNVGDSRVYKIDGQGMTQLTKDQTFVQKEIDA
                     GRMTPDEARVHPQRNVLLQCVGASEVIIPEFTQGEYKSGEVYMLCSDGFRHVITEEEF
                     YKLIDPEKMETERALKDAAVYCTELNKSRQEKDNISVILLKVC"
     gene            4671..6416
                     /locus_tag="ACZPD4_02875"
     CDS             4671..6416
                     /locus_tag="ACZPD4_02875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1378055.1"
                     /translation="MLEIGSIIDGKYKILNKIGQGGMSVVYLAMNERANKQWAIKEVR
                     KDGVKDYEVVRQGLIAETDILKRLNHPHLPSIIDVIDREDTFLIVMDYIEGKALDYWL
                     KKEGAQPQERVVEWAKQICDVFGYLHSRKPPIIYRDLKPANVMLKPDGQIMIIDFGTA
                     REFKETSIEDTSCLGTQGYAAPEQYGGHGQTDARTDIYTLGATMYHLLTGHNPSLPPY
                     EMYPIRRWNPALSSGLEEIVLKCTQRNPNDRYQSCAELMYALEHYGELDQEYRKRQMR
                     KWRTFVAMCSLTAASLLGCVGFKVGETMTTKSSYEGYIKEAANSPEQSEREKYYTDAI
                     KIDPRRGEAYHNLLQLCIRGADGSENDFDREETARLTSVLGYKGSGNRTNESYFEGNK
                     EEYDEFAFQMGLAYFYSYEGGEKSGKSMSQTWFEKAAESESLDKDKKELSKRFASIAA
                     YYASLDSVDESGYSTTSYGDYWIDLVSLTDGDLTSVVNPETALVMYKELVYRIDENAL
                     DFKRAGVTKGELLGELEETKKTLETTSFASTNANQTDINEQKKQEILNNISSAKEHVQ
                     VAFESRGTGGDADAE"
     gene            6406..7131
                     /locus_tag="ACZPD4_02880"
     CDS             6406..7131
                     /locus_tag="ACZPD4_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378056.1"
                     /translation="MQSEQIINILHIGFIICLVLTILFAALSVFFFFQFKIRDVFNAI
                     TGRAQRKSVQQMEEENAKTGKLRQDYYSAPTSSDLYTTPSGRIPPVMSAQQAAGGQAD
                     GAAYTEQVYHSADPEGREHTMQLHTAQQAASDNGGSEATTLLNSGSEETTLLNSGSEE
                     TTLLNSGSEETTLLNSGSEETTLLNGRNGGTAAQRNAGFGETTLLTPEMEASFVQAKT
                     NEKPEWNFVIKKEIMEIHTNEII"
     gene            7188..7697
                     /locus_tag="ACZPD4_02885"
     CDS             7188..7697
                     /locus_tag="ACZPD4_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378057.1"
                     /translation="MKPRTVKKGFLGVMGLFLLSALTPALIPFAVDKNGDLTQIGYAA
                     GALFWLGLLLGIAAYLFLARKAKPLTEQAGKRKLPSVICFFSNTPAIAADAVMIVSIA
                     ATIYCALHIEVNDFLAITALVFALAGIYAHFLFNGNVYFYIWNSRNRQRKRQTKERQN
                     KKEQEGMKA"
     gene            7694..12118
                     /locus_tag="ACZPD4_02890"
     CDS             7694..12118
                     /locus_tag="ACZPD4_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378058.1"
                     /translation="MKKKQKLKERICALVLALAMVLTWVLPDAGMTVQAAGGTEVEYT
                     VSVTDEDGALADVSVSAQTGDTAAVTAKTGQNGKATLILTKDAEYTVTLAKEGYETHT
                     ANVTPTEQNTQMNQKLRFKPFTITASDSTIAGQNVTLQIANPVAGVSYQWTANGASAA
                     PTSGTGTSFNITCAAAGTVNVTAAAKGQTASKSIIVNKKTINTSDISISLDPDQGAVN
                     KISVTVAGFPADAEGQVTLQYSYQGSTYTKTASKTKDITSVVIVLNAEQDVFFGKLNC
                     TVSYANDALYNDFSGVNKDAVYRKTHELTSEQKIELDTDNDGKKEEVYGQEITYGECI
                     DKTVELKAEDVKDRVVTYSFDENDTESQNIVTISEDGKISLKEDGHSGTAKVIVKAAE
                     TDDYAAAQMEYYIVAKPKAITVDVSKNLTVKDASKVYDGSADITVGAVVDGTLLVNGD
                     DGTNAEKDVLEIEGVKGTLWNTTEDDADPSTVSNEVKITADSFKEAELSSKWYEITYK
                     GENVFEGKTTVTRRTLYLGTSDIADCNYELDMFNEIQSAQAKVVTLNKDADVKDNGFV
                     SGENAENTGIEIPHVTVELNKVTYDEVDGKILIAVTEENAPYKDAIVPNITTENAGLN
                     YRYEVIPAGNAMYPDNIGDLTVKQQSKVEKDILDALEITAVLPNGEPTPASDRLYYQA
                     GADELWTNGSEVGTKLIFKIKESSEMSKLYDQVKVYVKSTDLIGKDGSDGVTFKEDTD
                     KNINVKINLKKASRPWTKTEKIDFTVHVDSTDPRFTFGQWGETKVASDTLRQVISFNQ
                     YKKSYYSFENVSASDVPQAVLASENAYSGVKASAYYVWKLDGPQASDVGFDEVNAKIE
                     AITLDQWVSLGEEEKSIPVIKAESGELKNLQGEYIVLAKVSDNVGHTCVYASNGVIVD
                     VEEPSISITGVDSSKYYNADIPFTVEVEDGKILNEETGKEDTVTTASIEKVTVQTYRD
                     DKETYKGEYPVEIENADDKKSGYTLEQIKGMAHKTIKDKVIADDKNDSSKSNNSNDVL
                     IKVTAQDCAGNTIEAKQALKIDTTAPEITVSYDNNDVVNDKYFDKARVMTIKYKERNF
                     DESKATFNLKIGDQTINGVTLTQLINSGESTYGIKGEFVENGDTQSDREEKDRTDDRT
                     VTYRLTFSKDNDYKIIPACEDKAGNTNKENKEDVTYVSGTKAKEEFTIDTKDPEISVK
                     YYISGSETSVSAEEANRLYTQENVKAVVSIKEHNFIIRDKFSNDPKQMTFTVTATEEL
                     EGEAVDTEAYATDANNQGDKKWQPAGVDIYEKEFEFTKDANYSLAIEYKDLAGNNVTY
                     APHFFTVDDTVPEGKVAYTTNSGEETWWNKFFDLITFNRFSNKDVPVSFESKDTTAGV
                     ATQEYYKAYEPMSKDEVKGLSDDEWTAGDGFTVEPDEQFVPYLKVTDKAGNVDYFSSE
                     FAVVADQTKPEIEITNLSTPRNGIFKGDVKLHISCRSL"
BASE COUNT         4155 a         2097 c         3032 g         2860 t
ORIGIN      
        1 atgaaatatt aaaggatgga attaaaattt atggataata agaagactga ccaatattat
       61 attgataaga ttattacaga cctcactttt attagaacac atacggagag cctgtcaaaa
      121 ttgaaattgg aagaggatga gattcttgtt gattctatta tgtttcggct aatacaggtg
      181 gcagaaaatt ccgagaaatt gacagaaaaa tttaaagaaa catatccatc cattccgtgg
      241 agagctatta aaggattgcg gaatcgaatt gtacatgaat atggaaatgt agaccttacg
      301 attgtatatg atacggtaaa aaaagatata ccggaattga tcggaatatt ggaaggaatc
      361 tgccgttgca aaaaagaata aaaagttccc tgacagcgag atgcaaaaga aacatctgcg
      421 gcggtcaggg gattttttat ttactgtatt cttgaatcag atcgtgtatt tttctataag
      481 cttcttcaga tgtgaagaat atttcatcgt caagcgtatc gcaaaaatca aaaaatgtaa
      541 aaagcagtcc gggttcttcg atgttcaggt agatcatccg gtctgtattt gaagtctgaa
      601 acatgaaata gccgtactgg cgtccggcaa agaggtaaaa ttcatggtcg atcatgccga
      661 tcgcccgctt caaaagccga ttatgctcgt tgagcatgga gtttgccaac tgccgaatca
      721 gaaagaggcg atcgcttaag ttcagcggag tatatgcata tgcaggatac tctccgacgc
      781 ggccggtatt taaaaacttt cttattcctt cgatagaaca gataaacgta aggtcggcag
      841 aggcgggaat tgattgaagt tcatgtaaat agttctgaag tctgtcgaga agttcgcttc
      901 ggtgtggaag atcttttaca atgtaagtct ctaacatgtc tcttgtcaaa aaccttgtca
      961 ggcacggaag catttgaaag aaataacctt cggttatgtt ctgcattgta ttttcgacat
     1021 actgtatttg tccgcctaaa tttgtgaccg ggcgaagcag gggtgttgtc tgcgccaggt
     1081 actgactgaa cagataggaa aataacttta gtgactccgg gtctttagta ataaagccgg
     1141 actgcatatc tgaagaaatc agacaggcat attcagtagt gataaccgca taaggaaata
     1201 aagcaaacgc tgaagttacg gcatcaatat catcatagta atagtaacag ttatattgat
     1261 aatgattact gtacagagga agaatatttc tcaggcagtt cagattataa ttttgccgtg
     1321 caaatgacac gttctggtta ttgttcaggc agataatgtg atcgatgtct acagaagagg
     1381 acgcataatc acctgctgta agaatgttca taagaaacgt gtaatccggc tggatgagca
     1441 gactgatctt tccacctgat tttttcagtt ccgaggcgat gatgtaaagg agcgagcgat
     1501 tgatctcaag atgagaatga agcaaggtag cattgtcggc gtaatcggac gcattggaag
     1561 gtgaagttgc cggaagctgc agtgtcggaa tacggaattc ttcaaaaaac tgttgtacgg
     1621 cttttctcct gtaatagttt tcgcgtccga tcagggcaat gtgatatgat tcttccatat
     1681 cggcctgttc tgaaggcgac aaatgcataa atttacacat tttttctacc atttctaaag
     1741 aagacggttt tcgttgccct tttataatct tgtacatatt ggatcgatcc aaattacagt
     1801 attgggcaag agcgtaagtt ttgatgtttt tattgtggat atgttctgat aataatgtcg
     1861 caaaatccgg cataatctta atctccctct cacatgtttt cctcggtaaa aaagaatact
     1921 cccacatggc aaaataccag ggggggggta ttactttttg aaagacaaac taatacagga
     1981 taattatata aaagcaaata taaaaaatct ataaaaatcc gacaaaaaag ctgaccacaa
     2041 aggttgacac atctttgaga acaaaggaaa accctatatt atacatataa tgacaaatat
     2101 tatatgtagc aggaggaaga gattatgggt aaaaaactaa cgaaaaaact gttggtattt
     2161 cttacactca tgtgcatgat tatcggcatg aatgtatcgg tatcagcaga aactggatcg
     2221 acgggaaata ccaaagcgat aagcgctgcg gaaaatccgg cggacggaac gcttcaggta
     2281 tggcttgagt atgaagatga taacggaaac agtgtagcgt tgtcaggcgg aagctgcttt
     2341 ttgatcaatg aagagtatgt actttccaac tatcatattt tcaatttgaa tgaagagatt
     2401 gctcctggtg tgacgatcag aagcgcggca atggaaacgt tgggattatc cgaattaaaa
     2461 gacaacgatc cgcatctgaa gatcagagta tatgcgaacc gtgacatgag cgtgctggca
     2521 acgatccatg acagtgtgca gagtcagacg atggactatg tagcaatgaa actgtcggat
     2581 aagatttatg acagaaaacc gcttgctttg ggagacagca gtacagttca gcagacacag
     2641 acagtaaacg cgtacggatt cccggaagac tcgatcgaaa ataaagagtt taatacggcg
     2701 gctgacgtat ctgcaacaga cggaacgatt tctaagctga cagtcggagg aagagctgat
     2761 ttcttcgagc acactgcaca gcttaaccat ggaaattccg gcggtcctct tgtagattcc
     2821 aacaagtcgg taatcggtgt taatacattt acatatggaa acagctcaga tcagaaatac
     2881 tacgcagtac agatcaacgc gatcaagagt ggtcttgaca cattcggtat ctcctatatt
     2941 aatgcagggg cacaggcaac aacacagact cctgatgaag aagagccgga agaaaaagaa
     3001 ggaccggacg cagatcttgt aacagcgctt aagagtgaaa ttacaaaggc gaaagcagtt
     3061 gatacgaaaa attacacaga agaaagtgta aaagttctca acgatacaat ctcagaagca
     3121 gaaaaagttg cgaacgatac agaggctaca gacgcacagg tgacatcggc aacatcagat
     3181 gtaaaagctg ctgttgaaaa tctggaagaa aaaagcggaa tgaacttcct gttgatcggc
     3241 ggaatcgcag caggagtgat cgttgttgta gtagtaatca ttattatcgt agtttcttct
     3301 aacagcaaga agaaaaagaa agcacaggca ggaaacggaa atgttcctcc gtcaggaatg
     3361 cctcagcagc cgaacggagc tgtagtacct ccgaaccgtg caccggaaca gccgaaagcg
     3421 ccttacgcac agccgtatca ggcagagccg gatggagcag gcgagactac tttgcttgat
     3481 tcgggagcag gcgaaacaac gttacttaac ggagcaggaa gcgcttatct gatcagaaag
     3541 aaaaacggtg agaagattac gatcaattca cagaactttg caatcggaaa agaaagaaga
     3601 agagtgaact attgtatttc cgataatact tctgtcagca gatatcatgt agtgatcaca
     3661 aagaaaggtt ctgattacta tgcggcagat cagaagtcaa gcaacttcac gtttgtaaac
     3721 ggagttcagt taaatccata tcaggaaaca ctgctgacag acagatcgac attgaagatt
     3781 tcggatgaag aatttgaatt tcatgcatca tgatctaaac gataaaagcg ctgattataa
     3841 ttgacaaaag agtaaagggc agtgagaggc agaatgggat ttttaagtgt tgtacatagt
     3901 gacagaggaa ttaaaaagaa tacgaatcag gattctgtct taatcaaaga ggcatccaca
     3961 gactacggcg acgttatgct tgctgtagtt tgtgatggaa tgggcggtct tgccaaaggt
     4021 gaggtcgcca gcgccgcttt gatcaaggca ttttctatgt ggtttgaagt tcagtttccg
     4081 gaacttcttt atcggagaag gacggctgat gggattaacc gtatggaatt ggaaaacgaa
     4141 ataaataaac tgatccttga tgtaaatcaa agaatcgccg atcacggaaa gatttcccat
     4201 attgcgatgg gaacaactgc ggcggtactt ctgatggcag aagggaaata ttatacgatg
     4261 aatgtgggcg actccagagt ctataaaatt gacggacagg ggatgactca gttgacaaaa
     4321 gatcagacgt ttgtacaaaa agagatcgat gcagggagaa tgacgccgga tgaggcgaga
     4381 gtgcatccgc agcggaacgt acttcttcaa tgcgtcggag caagcgaggt gatcattccg
     4441 gagtttacac agggagaata taagtcgggc gaagtttata tgctctgctc tgacggattt
     4501 cgccatgtga tcacggaaga ggaattttat aaattgatag atcctgaaaa aatggagacg
     4561 gaaagagcgc tgaaagacgc agcggtctat tgtacggaat tgaataaatc acgtcaggag
     4621 aaagataata tttcagtcat tctccttaag gtgtgttaga ggataaatat atgctggaaa
     4681 taggatcaat tatagacggt aaatacaaaa tactgaataa gatcggtcag ggcggaatga
     4741 gcgtcgttta tcttgcgatg aatgaaagag cgaataaaca atgggcgatc aaagaagtcc
     4801 gaaaagacgg cgtgaaagat tacgaagttg tacggcaggg actgatcgcg gaaaccgata
     4861 ttttgaaacg gttaaatcat ccgcatttac cgagtatcat cgatgtgatc gacagggagg
     4921 atacgttcct gatcgtaatg gattatatcg aaggaaaggc gctcgattac tggctgaaaa
     4981 aagaaggcgc gcagccgcag gaacgggttg tggaatgggc gaaacagatc tgtgatgtgt
     5041 tcgggtatct tcattcgagg aaaccgccga tcatttacag agacttaaaa ccggcgaacg
     5101 tcatgctgaa gccggacggg cagatcatga tcatcgactt cggaacggcc cgcgaattta
     5161 aagagacaag tattgaagat acgtcttgtc tcggaacgca gggatatgca gcgcccgaac
     5221 agtacggcgg gcatgggcag acggacgccc gtactgatat ttatacgctg ggtgcgacga
     5281 tgtaccattt gctgacggga cataatccaa gtcttccgcc gtacgagatg tatccgatcc
     5341 gaagatggaa tccggcgctc tcgtcaggat tggaagaaat cgtactgaaa tgtacgcaga
     5401 gaaatccgaa cgaccgttat cagtcgtgcg cggaactgat gtacgcgctg gagcattacg
     5461 gagaattaga tcaggaatac cggaaacggc agatgaggaa atggcggacg ttcgttgcaa
     5521 tgtgttcgct gactgccgca tcacttctcg gatgcgtcgg atttaaagtc ggggagacga
     5581 tgacgacaaa gagcagttat gaaggatata taaaagaggc ggcgaactcg ccggaacaaa
     5641 gtgaacggga aaaatattat acagatgcaa ttaagataga ccctcgaaga ggagaagcgt
     5701 atcacaatct tttgcagtta tgcatccgcg gagcagacgg gtcggaaaat gattttgaca
     5761 gagaagagac agcgcggctt acgtcggttc ttggatataa aggcagcgga aaccggacaa
     5821 atgaaagtta ttttgaagga aataaagaag aatacgacga attcgctttt caaatgggac
     5881 tggcgtactt ttattcatat gagggaggag aaaagagcgg aaaatcaatg tctcaaacat
     5941 ggtttgaaaa agcggcggaa agcgaaagcc tcgataaaga taaaaaggaa ttgtcgaaaa
     6001 gattcgcaag tatcgcggcg tactatgctt ctcttgattc agtggatgag agcggataca
     6061 gcacaacttc ttacggagac tactggatcg atcttgtcag cctgacggac ggagatctta
     6121 caagtgtggt aaatcctgag acagctcttg taatgtataa agaacttgta tacaggatcg
     6181 acgaaaacgc acttgatttc aaacgtgcgg gagtgacaaa aggcgaactt ctgggagaac
     6241 tcgaagaaac gaaaaagacg ctggagacaa cgtcgtttgc atcgacgaat gcaaatcaga
     6301 cggatatcaa cgagcagaaa aaacaggaga ttctgaacaa tatcagcagc gcgaaagagc
     6361 atgtgcaggt agcatttgaa tcccggggaa caggagggga tgctgatgca gagtgaacag
     6421 atcatcaata tacttcatat cggatttatt atctgtcttg tgctgacaat tttatttgcg
     6481 gcgctgagcg ttttcttttt ctttcaattt aaaatacgcg atgtgtttaa cgctatcacg
     6541 ggaagggcgc agcgaaagag cgttcagcag atggaagaag aaaatgcaaa gacaggaaag
     6601 ttaagacagg attattattc cgcgccgaca agttcagacc tttatacgac gccaagcgga
     6661 aggatacctc cggttatgtc tgcgcagcag gcagcaggcg gacaggcaga cggcgcggcg
     6721 tacacagaac aagtttacca ttcggcagat ccggaaggaa gagaacatac aatgcagctt
     6781 cacacagcac agcaggcagc gtctgataac ggaggaagtg aagcgacaac attgctgaat
     6841 agcggaagcg aagaaacgac tctgctgaac agcggaagcg aagagacaac tctgctgaac
     6901 agcggaagcg aagaaacgac tttgctgaac agcggaagtg aagaaacgac attgctcaac
     6961 ggcagaaacg gcggcacagc ggcacaaaga aatgccggat tcggagaaac aactttattg
     7021 acgccggaaa tggaagcatc gtttgttcag gcaaagacaa atgaaaaacc ggaatggaat
     7081 ttcgtcatta aaaaagaaat tatggaaatc catacgaatg agatcattta aagaaaacaa
     7141 aaaaagatag taatcgattt atcttgcagc agaaagaagg aaacagattg aaaccaagaa
     7201 cagtaaaaaa aggatttctc ggtgtgatgg gattatttct cctgtctgca ctgactccgg
     7261 cgctgattcc gtttgccgtt gacaaaaacg gcgatcttac acagatcgga tatgcggcgg
     7321 gggcattgtt ttggctcggt cttctgcttg gaatcgcagc atatttgttt ttggcgagaa
     7381 aggcgaagcc tctgacagaa caggcaggaa aacggaaact tccgtcagtg atctgctttt
     7441 tcagtaatac gcctgcgatt gcggcagacg cagttatgat cgtgtcgatc gcagcgacaa
     7501 tatattgtgc gcttcatatc gaagtgaatg attttctggc aattacggcg ctggtatttg
     7561 cgcttgcagg gatttatgca cattttctgt ttaacggaaa cgtgtatttc tatatatgga
     7621 acagccgaaa cagacagagg aagagacaga caaaagaacg gcaaaacaaa aaagaacagg
     7681 aagggatgaa agcatgaaaa agaagcaaaa actaaaagaa aggatatgcg cgctcgtcct
     7741 tgcgctggcg atggtactga cttgggtact gccggatgca ggtatgacgg ttcaggcggc
     7801 aggcggaaca gaagtagagt acactgtttc cgttacagat gaagacggcg cgttggcaga
     7861 cgtttcagtg tcggcacaga caggtgatac tgcggctgtg acagcgaaaa ccggacaaaa
     7921 tggtaaagcg acgctgattc tgacaaaaga tgctgagtat accgttacat tagcaaaaga
     7981 agggtatgaa actcacacgg caaatgtaac gcctacggaa caaaatactc agatgaatca
     8041 gaaacttaga tttaaaccgt ttacgatcac agcatctgac agtacaatag ccggacagaa
     8101 tgtaactttg cagatcgcaa atccggttgc aggagtgagt tatcagtgga cggcaaacgg
     8161 tgcatcagct gcgccgacaa gcggaacagg cacatcattt aatatcacat gtgcggcagc
     8221 gggaacggta aatgtgacag cggcagcaaa aggacagacg gcatcaaaaa gtattattgt
     8281 taataagaaa acgataaata catctgatat ttcgatcagt ttagatcctg atcagggcgc
     8341 tgtaaataaa atcagcgtaa ctgttgcagg ttttccggct gatgcggaag gtcaggtgac
     8401 attacagtat tcttatcagg gaagcacata tacgaaaact gcgtctaaaa caaaagatat
     8461 aacgtctgta gtaattgttc tgaatgcaga gcaggatgta tttttcggaa aattaaattg
     8521 tacggtaagt tatgcaaacg atgcattata caatgatttt tcgggagtga ataaagatgc
     8581 tgtttacaga aaaacgcatg agttgacttc tgaacagaaa atcgagctcg atacagacaa
     8641 tgacggcaag aaagaagaag tttatggaca ggaaatcaca tacggtgagt gcattgataa
     8701 gactgtcgaa ttgaaggcgg aagatgtcaa agacagagta gttacatata gttttgatga
     8761 aaatgacaca gaaagccaaa atattgttac gatcagtgaa gacggcaaga tttccttaaa
     8821 ggaagacgga cattcgggaa cagcgaaagt gatcgtaaaa gcagcagaaa ctgatgatta
     8881 tgcggcagca caaatggagt attacatcgt tgcgaaaccg aaagcaatca ctgtagatgt
     8941 aagcaaaaac ctgacagtga aagacgcgtc taaggtgtat gacggatcag cggatattac
     9001 agtcggggca gtcgttgacg gaacacttct cgtaaacgga gacgatggaa cgaatgctga
     9061 aaaagatgtt ttggaaatcg aaggtgtgaa aggaacactt tggaatacga cagaagatga
     9121 cgctgatcca tctactgtaa gtaatgaagt taaaataaca gcggattctt tcaaagaagc
     9181 agaattgtca tcgaaatggt atgagattac ttataaaggt gaaaatgttt ttgaagggaa
     9241 aacaactgtt acgagaagaa cattgtatct tgggacaagt gatattgcag attgtaatta
     9301 tgaattagat atgtttaatg aaattcaatc tgcacaagcg aaagtagtta ctcttaataa
     9361 agatgcagac gtaaaagaca atggatttgt gtcaggagaa aatgcggaga atacaggaat
     9421 tgaaattccg catgtaacag ttgaacttaa taaggtaact tatgacgagg tcgatggaaa
     9481 aattcttatt gcagtaacag aagaaaatgc accatataaa gatgcgattg ttccgaatat
     9541 aacaacggaa aatgcgggat taaactacag atatgaagta atcccagcag gaaatgcaat
     9601 gtaccccgat aacatcggag atctgacggt aaaacaacag tctaaagttg aaaaagatat
     9661 tttagatgcg ctggagataa cagcagtatt accaaacggt gagcctacac cggcgtcaga
     9721 tcgtctgtat tatcaggctg gagcagacga gctttggaca aacggaagtg aggtcggaac
     9781 aaagctgatc ttcaaaataa aagaaagctc tgagatgtct aagttatatg atcaggtaaa
     9841 agtctatgtg aagagcacgg atttaatagg aaaagacggc tctgacggag ttacatttaa
     9901 agaagacaca gataaaaata ttaatgtaaa aataaacctc aagaaagcat ctcgtccgtg
     9961 gacaaagaca gagaaaattg attttacagt tcatgttgac tcgacagatc cgagatttac
    10021 gtttggtcaa tggggagaga caaaggttgc aagcgatact ttaagacagg ttatttcttt
    10081 caatcagtat aaaaagagct attattcatt tgaaaatgtc agcgcaagcg atgttcctca
    10141 ggctgtactt gcaagtgaaa atgcatacag tggtgtgaag gcaagcgctt attatgtatg
    10201 gaagcttgac ggaccgcagg cttcagacgt agggtttgat gaagtaaatg caaaaattga
    10261 agcaatcaca ttagatcagt gggtttcact tggagaagag gaaaaaagta ttccggtaat
    10321 caaagcggaa agcggagagc tgaagaatct tcagggcgag tatatcgttc ttgcgaaagt
    10381 atcagataat gtaggacata catgcgtata tgcatcaaat ggggtgatcg ttgatgtaga
    10441 agagccgtcg atttcaatta caggagtaga cagttccaaa tattataatg cagacattcc
    10501 gtttactgtt gaggtagaag atggaaagat cttaaatgaa gaaacaggaa aagaggatac
    10561 tgtaacaaca gcaagtatcg aaaaagtaac ggttcagaca taccgcgacg ataaagagac
    10621 ttacaaagga gagtatccgg tagaaattga aaatgcagat gataaaaaat caggttacac
    10681 attagagcag atcaaaggga tggctcataa gacgatcaag gataaggtaa tagctgatga
    10741 caagaacgac agcagcaaaa gcaacaacag caacgatgtg ctgatcaaag tgacagcgca
    10801 ggattgcgca ggaaatacga tcgaagcaaa acaagcgctg aagatcgata cgactgctcc
    10861 ggaaatcaca gtttcttatg ataataatga tgtagtaaat gataaatatt ttgataaagc
    10921 tcgtgttatg acaatcaaat acaaggaaag aaactttgat gagagcaagg cgacgttcaa
    10981 tctgaaaatc ggagatcaga cgataaacgg cgtgactctg acccagctta taaactccgg
    11041 tgagagtact tatggtatca agggtgagtt tgttgaaaat ggagatacgc agtcagatag
    11101 agaagagaaa gacaggacag atgacagaac ggtgacgtac agacttacgt tctctaaaga
    11161 taatgattat aagattatac cggcgtgtga agataaagca ggaaatacaa ataaagagaa
    11221 taaagaggat gtaacatatg tgtcgggtac aaaagccaaa gaggaattta cgattgatac
    11281 gaaagatccg gaaatttctg tgaaatatta tatcagcgga agcgaaactt ctgtatccgc
    11341 agaggaagcg aacagactgt atacacagga aaatgtgaaa gcagttgtca gcatcaaaga
    11401 acataatttc ataataagag acaaattctc aaacgatccg aagcagatga cgtttaccgt
    11461 gacggcgacg gaagaactcg aaggtgaagc agtagataca gaagcttacg caactgatgc
    11521 aaataatcaa ggtgataaga aatggcaacc ggcaggagtc gatatatatg agaaggaatt
    11581 tgaatttacg aaagatgcaa attattctct tgcaatcgaa tataaggatc ttgcaggaaa
    11641 caacgtgaca tatgcgccgc atttctttac agtagacgat acagtaccgg aaggaaaagt
    11701 tgcttatacg acaaacagcg gcgaagagac atggtggaat aaattctttg atttaattac
    11761 atttaacaga ttcagtaata aagacgttcc ggtatctttt gaaagcaagg atacgacggc
    11821 aggagtagct acacaggaat attataaagc ttacgagccg atgtcaaaag atgaggttaa
    11881 aggactttca gatgacgaat ggacagcagg agacggattt actgttgaac cggacgagca
    11941 gtttgttccg tatttgaaag taacagataa ggccggcaac gtagattatt ttagttctga
    12001 atttgcagtt gtggcagatc agacgaaacc ggagattgag attacaaact taagcacgcc
    12061 gagaaacgga atattcaaag gtgatgtaaa actccatatc tcgtgcagat ccttatgatt
    12121 gtacatattg gtcattatac ggcg
//
LOCUS       DBMDXF010000041        12075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1512_length_12075_cov_1.517371, whole genome shotgun sequence.
ACCESSION   DBMDXF010000041 DBMDXF010000000
VERSION     DBMDXF010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_12075_cov_1.517371"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(57..503)
                     /gene="dtd"
                     /locus_tag="ACZPD4_02895"
     CDS             complement(57..503)
                     /gene="dtd"
                     /locus_tag="ACZPD4_02895"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1378059.1"
                     /translation="MKFVIQRVKQASVKVEGSVIGEIEKGYLVLIGVSDKDTEAVADK
                     MIKKMIGLRIFEDAEGKTNLSLADVGGSLLLVSQFTLYANCKKGNRPSFIEAGAPDKA
                     NQLYEYIIEESKKSVSVVQTGRFGAEMEVSLINDGPFTILLDSEML"
     gene            complement(536..1162)
                     /gene="sigH"
                     /locus_tag="ACZPD4_02900"
     CDS             complement(536..1162)
                     /gene="sigH"
                     /locus_tag="ACZPD4_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HIR1378060.1"
                     /translation="MEQYEKLTDEQLIEKLHSGDRDVTDYLMEKYKMMVRKKARAMYL
                     IGGETEDLIQEGMIGLIKAVRNFDSGMGRSFAGFAETCVLRQMYTAIEASNRKKHMPL
                     NSYISLYEESETNKNGTKLPLIDLIEPDRENDPEALYFGREYTEAFIERLKENLSPME
                     EQVLYLHLTGTDYKKIAELLKKSPKSIDNALQRIKKKTEKMLKTEIRA"
     gene            complement(1197..1973)
                     /gene="rlmB"
                     /locus_tag="ACZPD4_02905"
     CDS             complement(1197..1973)
                     /gene="rlmB"
                     /locus_tag="ACZPD4_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1378061.1"
                     /translation="MERKTIEKIEETKEHTLVIEGRNAVLEAFRSGRTIDKLFVLDGC
                     QDGPVRTIVREAKKHGTILNFVGKERLSQLTQTGRHQGVIAYAAAYDYAEIDDMLKLA
                     EKRGEDPFLIVLDGIEDPHNLGAIIRTANIAGAHGVIIPKRRAVGLTATVAKTSAGAL
                     NYTPVAKVTNLVKTMEELKEKGIWFVCADMDGQSMYDMNLTGPIGLVVGNEGDGVSRL
                     VKENCDFVASIPMKGEITSLNASVAGGVLAYEIVRQRLAK"
     gene            complement(1954..2421)
                     /locus_tag="ACZPD4_02910"
     CDS             complement(1954..2421)
                     /locus_tag="ACZPD4_02910"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR1378062.1"
                     /translation="MEKSLGYGFEYYMDLIPGMKQADPKASSSIVLAYIGDCVFDLII
                     KMRVVGRGGRQVHKLHEETSRYVQASAQSFMMRAIQEHLTEEEHAVYRRGRNTRSVSA
                     AKNQSITDYRRATGFEALVGYLYLKKEYKRLVELVTIGLESMEKELENGEKND"
     gene            complement(2406..>2605)
                     /locus_tag="ACZPD4_02915"
     CDS             complement(2406..>2605)
                     /locus_tag="ACZPD4_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR1378063.1"
                     /translation="LTDVLGIIVIKEEEMLDSEIEEMIEKRQAARKDRNFALADQIRD
                     ELLAKGIILEDTREGVKWKKA"
     assembly_gap    2606..2615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2616..3786)
                     /gene="cysS"
                     /locus_tag="ACZPD4_02920"
                     /pseudo
     CDS             complement(2616..3786)
                     /gene="cysS"
                     /locus_tag="ACZPD4_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427965.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
     gene            complement(3773..4459)
                     /gene="epsC"
                     /locus_tag="ACZPD4_02925"
     CDS             complement(3773..4459)
                     /gene="epsC"
                     /locus_tag="ACZPD4_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR1378064.1"
                     /translation="MGIVSYIKEEIRVVRERDPAIKSNMEVLLYPSFRVILRYRVAHK
                     LYLKKHYFWARWISQRAARKTGIEIHPGAKIGRGLFIDHGSGVIIGETTVIGDNVTLY
                     QGVTLGGTGKEQGKRHPTLEDNVMVSAGAKILGSFTIGENSKIGAGSVVLEEVPPNCT
                     VVGVPGRIVKMDNKKVPRSDMDQIHLPDPVLSDIKRLQEENLRLHKELKRLVKEIRCV
                     EKKGDYDEDL"
     misc_binding    complement(4518..4738)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4852..6048)
                     /locus_tag="ACZPD4_02930"
     CDS             complement(4852..6048)
                     /locus_tag="ACZPD4_02930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1378065.1"
                     /translation="MIIRKLEQSEHIATRKLWEEIFPEDTKAFLDYYYYIKAAKNQIY
                     VVEEDGRICSMLQLNPYRIRLEDKSFPSEYIVAVATKKEYRSRGYMRALLNTVLERMH
                     SEKVPFTFLMPAAEAIYRPYDFRYIYDQCMQEVKKDETADDRNRESEPQKEGTLEFSD
                     AGLWDAEEMADFFEEHFSDSWQVYAQRDTAYYQTMILEQQSEKGGVRLMRENGVLKGF
                     YAYALEEGLEVREPLYLNQFEGEFERSMQMLLDKSNIRKADQNENRVQQSLRIYAPLN
                     KKSCKMRSMIMARIVCLPEFLKAMIVDETETLECSFAVLDSILYKNSCVWKLTSVQGE
                     KEIHVQETEDSQGVFPIADLTEYLFGRIDLEELREREGVICTAELGEELEKIEKLTRV
                     YFNEVV"
     gene            complement(6049..6600)
                     /gene="ispF"
                     /locus_tag="ACZPD4_02935"
     CDS             complement(6049..6600)
                     /gene="ispF"
                     /locus_tag="ACZPD4_02935"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1378066.1"
                     /translation="MRVGMGYDVHRLAEDRKMILGGVEIPYEKGLLGHSDADVLVHAI
                     MDALLGAAALGDIGKHFPDTDPEYKGISSIRLLKHVGGLLDENRYMIENIDATIIAQR
                     PKMRPYIDTMRQNIADALCIEIDQVNVKATTEEGLGFTGDGEGISSQAICAIEKYTNY
                     SSTDVSSDCGMCGGCCSRIGRNE"
     gene            complement(6603..7229)
                     /locus_tag="ACZPD4_02940"
     CDS             complement(6603..7229)
                     /locus_tag="ACZPD4_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378067.1"
                     /translation="MNKKAWYTFRIVLGGYLAWLGIRMLIEMVNEKPNNMVFMCVMSV
                     IFILIGLAYAIYYVKKMFDLKKEDHRIEEEDKEAFDAGKDQADRKRRKNVLPEIGPNE
                     EMKKEAEQGKTDKETAQSGNNIEKEAEKLDDKAEGETEKSGYKAGSETEQSDDKVKKE
                     AEKPNDKNEEDAEDEMIKEGGKNQEPELAMEEFIEETETEIENDYEER"
     gene            complement(7348..7439)
                     /locus_tag="ACZPD4_02945"
                     /pseudo
     CDS             complement(7348..7439)
                     /locus_tag="ACZPD4_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048813205.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            7766..7838
                     /locus_tag="ACZPD4_02950"
     tRNA            7766..7838
                     /locus_tag="ACZPD4_02950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7799..7801,aa:Val,seq:tac)
     gene            7876..7949
                     /locus_tag="ACZPD4_02955"
     tRNA            7876..7949
                     /locus_tag="ACZPD4_02955"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7910..7912,aa:Met,seq:cat)
     gene            complement(8147..11902)
                     /locus_tag="ACZPD4_02960"
     CDS             complement(8147..11902)
                     /locus_tag="ACZPD4_02960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1378068.1"
                     /translation="MSNVKRVFVEKKPEFAVAAKELGHEIRHYLGITDVTGVRVLIRY
                     DVENISDETFEKACQGVFAEPPVDILYKETFNMSDADHSFSVEYLPGQFDQRADSAEQ
                     CVRFIKEDETPVIHTATTYVIEGTISEEEFAAIKNHCINPVDSREAGEEKPETLVTVF
                     DDPEDVIVFEGFKEMKEDELEKLYSSLGLAMTFKDFLHIRNYYKDEEHRDPTMTEIRV
                     LDTYWSDHCRHTTFSTELKEVSFNEGFYKKPIEDTYKAYLKDHSEIFAGREDKFVCLM
                     DLALMAMRRLKKEGKLQDQEKSDEINACSIVVPIKVDGVEEEWLINFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRTYVYQAMRVTGAADPTVSVEETLKGKLPQKKLVRGAA
                     SGYSSYGNQIGLATGLVKEIYHPDYVAKRMEIGAVLGAAPRRAVIRENSDPGDIIILL
                     GGRTGRDGCGGATGSSKVHTEESIETCGAEVQKGNPPTERKIQRLFRREEVSCLIKKC
                     NDFGAGGVSVAIGELADGLRVELDKVPKKYAGLDGTEIAISESQERMAVVVDPKDVEK
                     FMSYAKEENLEATEVAVVTEEPRLVLVWRGKEIVNLSRAFLDMNGAHQETAVEVEIPR
                     QEESLFVKTAVSDVKEKWLDVLSDLNVCSQKGLVEMFDGSIGAGSVFMPHGGKYQMTE
                     TQAMVAKVPVQNGTTDSVSMMSYGFDPYLSSWSPYHGAAYAVVESVAKIVAAGGDYSK
                     IRFTFQEYFRRMSEDPKRWSQPFAALLGAYAAQIGFGLPSIGGKDSMSGTFQDIDVPP
                     TLVSFAVDMAVEKEIITPELKEAGNKLVWLKAARDEYDLPVYSRIMEQYSKFTEDIRN
                     GRVVSAYALDRHGVVSAVSKMAFGNQMGVCLESSLDKDAIFAPAFGDIVAEVKADTFE
                     ELVGEYTLIGEVTNEKAFAYGDVKIGMDDALDAWTKPLEKVFATKSAESSDIPVEEKL
                     YDASTVHVCSHKIGQPTVFIPVFPGTNCEYDSARAFERAGANVITKVFRNLDAEDIRS
                     SVEEFEKAISKAQIIMFPGGFSAGDEPDGSAKFFATAFRNAKIKEAVDKLLNERDGLA
                     LGVCNGFQALIKLGLVPYGQIVGQTDDSPTLTYNTIGRHISKMVYTKVVTNKSPWLAE
                     AKLGGVYTNPASHGEGRFVASEEWIDKLFANGQVATQYCSPEGHITMDEEWNVNGSYH
                     AIEGITSPDGRVLGKMAHSERRGNSVAINIYGEQDLKIFESGVKYFK"
BASE COUNT         3110 a         3174 c         1753 g         4028 t
ORIGIN      
        1 gtatttcact gactctcctt aaatttcaaa caattagttt ttttccgatg atcctatcac
       61 aacatttccg aatcaagcaa aatcgtaaac ggaccatcat tgatcaaact cacttccatt
      121 tctgctccaa agcgacccgt ctgtacgact gacacacttt tcttactctc ttcaattata
      181 tactcatata actgatttgc tttatccggc gcccctgcct caataaaact cggccgattc
      241 ccctttttac aatttgcata aagcgtaaac tgtgatacaa gcaaaagact tccgcccaca
      301 tcagctaacg acaaatttgt ttttccctct gcatcttcaa aaatccgcag tccgatcatt
      361 ttctttatca ttttatccgc aactgcttcc gtatctttgt ctgaaacgcc aatcaatacg
      421 agatatcctt tttcaatctc tcctatcacg cttccttcca cttttacaga tgcctgtttt
      481 acccgctgta ttacaaattt cataaccttt tacccgcttt ctctatttga atttctcagg
      541 ctcttatttc tgttttgagc attttttctg tttttttctt aattctttga agagcattat
      601 caatcgattt cggacttttt ttcaacaatt ccgcgatttt tttataatca gttcctgtta
      661 agtgcaaata aagaacctgt tcttccatcg ggcttaaatt ttctttcaat cgttcaataa
      721 aagcttctgt gtactctctg ccaaaataca atgcctcagg atcattttcc cggtcaggct
      781 ctatcagatc gatcagaggc agttttgtac cgtttttatt cgtctcgctt tcttcataaa
      841 gtgaaatata agaattcaga ggcatatgtt tttttcgatt tgacgcctca atagcggtgt
      901 acatttgccg aagtacacat gtttccgcaa accctgcaaa tgacctgccc atgccgctgt
      961 caaaatttct taccgccttg attagtccga tcatgccttc ctgaataaga tcttctgttt
     1021 ctcctccgat cagatacatt gcgcgtgcct tcttgcggac catcatttta tatttctcca
     1081 tcagataatc tgtcacatcc cgatccccgg agtgcagttt ttcaatcaac tgttcatccg
     1141 tcagcttttc atattgttcc atcctttaaa acacccttca aacactgctt tacatcttat
     1201 tttgcaagtc tctgtctgac aatctcataa gcaagaactc cgcctgccac ggaagcattc
     1261 agagaggtaa tctcgccttt catcggaatt gacgcaacga aatcacaatt ttcttttact
     1321 aatcggctga ctccatcccc ttcatttccg acaacaagtc cgatcggtcc tgtgagattc
     1381 atatcataca ttgattgtcc gtccatatcg gcgcagacaa accaaattcc tttttctttc
     1441 aattcttcca ttgtctttac aagatttgtc acttttgcaa ccggagtata attcaacgcc
     1501 cctgccgaag ttttcgctac ggtcgccgtc aaaccaaccg ctctcctctt cggaatgatc
     1561 acgccatgag ctccggcaat attggctgtt cttataattg cccccaaatt atgaggatct
     1621 tcaatcccat ctaatacgat cagaaacgga tcttctcccc tcttttccgc aagtttaagc
     1681 atatcgtcaa tctctgcata atcataagcc gcagcgtaag caattacgcc ctgatgcctt
     1741 cctgtctgcg taagttgaga taatctttct tttccgacaa aattcaagat tgttccatgc
     1801 ttttttgctt ccctgacaat cgtgcgtacc ggaccatcct gacatccgtc caacacaaac
     1861 agcttatcga tcgtccttcc agagcgaaac gcttccagta ctgcgtttcg tccttcgatc
     1921 actaatgtat gttcttttgt ttcttcaatt ttttcaatcg tttttctctc cattttccaa
     1981 ttccttttcc atactttcca gtccgatcgt tacaagctca acaagccgtt tgtactcctt
     2041 tttcaagtaa agataaccca ctaacgcctc aaaccctgtc gctcttcgat aatcggtgat
     2101 cgactgattt tttgcagccg aaacgctcct tgtattcctt ccccttcgat ataccgcatg
     2161 ttcttcttct gtaagatgtt cctgaattgc ccgcatcata aaagactgag cagacgcctg
     2221 cacatacctg cttgtctctt catgcagttt atgcacctgc ctgccgcctc tgcctaccac
     2281 tctcatctta ataataagat caaagacaca atctcctata taggctaaaa caatagagga
     2341 acttgctttt gggtcggcct gcttcattcc gggaatcaga tccatataat actcaaatcc
     2401 atatcctaag cttttttcca ttttactccc tctcgcgtat cttcaagaat aatccctttt
     2461 gccaggagct cgtctctgat ctgatctgcc agagcaaaat tacgatcttt ccttgcagcc
     2521 tgacgttttt ctatcatctc ttcgatttcc gaatctaaca tttcctcttc ttttataacg
     2581 ataataccca aaacatctgt cagcannnnn nnnnntttag cttgctccgt cggcagtcgt
     2641 gttcatatat ttgacaagat caaaaatagc cgctatcgca tcggcagtat tgaaatcatc
     2701 atccattgcc ctttcaaaag cttctacata ttctgatgat ttctcaaaca atctcttctc
     2761 cgactctgtc attttctctt ctgacgtgtt tgaaataagg aatttcaagt tatctgccgc
     2821 agtaaggatt ctctccaaag aattttttgc tgcttccatc aactctgcac taaaattaag
     2881 cggacttctg tagtgtgcac tcaacataaa aaatctcaag atctgaaggt catacttttc
     2941 tccgatctct cttactgtaa aaaagttacc gagtgatttt gacatctttt tattatcaat
     3001 attaagaaat ccattgtgca tccaatattt tgcaaattct tttccgttcg cggcttcgct
     3061 ttgagcaatc tcgttttcat gatgcggaaa tatcaaatcc tctccgccgg catgaatatc
     3121 aatttgttct ccgaggtatt ttttagacat ctccgagcat tcaatatgcc atcccggacg
     3181 gccctcactc cacggagatt cccaggcagg ttctccctcc tttttcggtt tccaaagcac
     3241 aaaatcaagc ggatcttctt tttcgtcttc tcctgtaacg agaagcgatc ttcctcccga
     3301 cctcaaatca tccagattct tatgcgaaag ctttccgtaa ccttcaaact ttcttgtacg
     3361 gtaataaacc gttccgtttt tttcataggc gtatcctttt tcaatcaatg tggaaatcat
     3421 ttccaccatg ccgcagattt cttctgttgc aagcggattt ttcgtggccg gttttacatt
     3481 cattgcttcc atgtcttttt tacattccgc aatatatctt ttcgatatct catcagcaga
     3541 aacgccttct tcaatcgctt ttttaataat tttatcatct acgtctgtaa agtttgatac
     3601 aaaattcact tcaaatcctt tatattcaaa ataacggcgt actgtatcaa atgcgatcat
     3661 cggtcttgca tttccgatat ggataaaatt ataaaccgta ggaccgcaca catacatttt
     3721 tactttgcct tcttccaatg gcacaaattc ttcttttctt tttgataatg tattataaat
     3781 cttcatcata atctcctttc ttttccacac atcttatctc tttcacaaga cgtttcaatt
     3841 ccttatgcaa tcttaaattt tcttcctgaa gcctcttaat atccgacaat accggatccg
     3901 ggagatgaat ttgatccata tcggatctcg gaactttttt attatccatt ttaacgatac
     3961 gccccggcac acctactacc gtacagttcg ggggcacctc ttctaaaaca actgatcccg
     4021 ccccaatctt tgaattttct ccgatcgtaa aagaacctaa tatctttgct cccgcgctta
     4081 ccatcacatt atcttccaat gttggatggc gctttccttg ttcttttccg gttccgccaa
     4141 gcgttacccc ttgataaaga gttacgttat cgccgatcac cgttgtctca ccgatgataa
     4201 ctccactccc gtgatcaata aacaagccgc gtccgatttt agctcccgga tgaatctcaa
     4261 tccctgtttt tcgcgccgct ctttgtgaaa tccatctcgc ccaaaaataa tgttttttta
     4321 aatataattt atgagccact ctgtatctga gtattacacg aaaacttgga taaagcaaca
     4381 cttccatatt tgattttatc gcgggatccc gttcccgcac aactcgtatt tcttctttaa
     4441 tataagatac aatacccatt ctgttctccc acaaaatatt ttttactttt accggttaaa
     4501 taaaaacttc gtctcagatc agagacgaag tttttccgcg gttccactct attaaaagat
     4561 ttttctttca ctcaacagcc tgtaacgtat gctaaacgaa tcaggctact tttcattcac
     4621 ccgatcagct cccggacgca cttcacaaac catctttgta caggaatgct ttcagcctcc
     4681 gacattccct ctctgcgaac tttcacaatc tgttactctt tccgttctct gctttttatt
     4741 cttcgacata taatttatat tatgcaaatt ccacattttt gtcaataccg cttttctttc
     4801 aaaggcaatc aaaatcgccg ttgcttcttt ttcttttgag cacttttcct ctcagacaac
     4861 ctcattgaaa tatactcttg tcagtttttc tattttctct aactcttcac caagctcagc
     4921 tgtacagatc acgccctctc tttcacgcag ttcttccaaa tcgatccttc caaacaaata
     4981 ttctgtcaaa tccgctatcg gaaatactcc ttgtgaatct tcggtctcct gtacatgtat
     5041 ttctttctcg ccctgtacgc ttgtcaattt ccatacacag ctgtttttat acaaaataga
     5101 gtcaagcaca gcaaaagaac attcaagagt ttctgtttca tcgacgatca ttgcttttaa
     5161 aaattcaggc aaacaaacaa ttcttgccat aatcatagac cgcattttac aacttttttt
     5221 atttaaagga gcataaattc tcagcgattg ctgtactctg ttttcatttt gatccgcttt
     5281 ccggatattg cttttatcca acagcatctg catcgaccgt tcaaattccc cttcaaattg
     5341 attcagatac aacggctctc gtacttcaag tccttcttct aaggcgtagg cataaaaccc
     5401 tttcaacaca ccgttttccc gcataagtct aaccccgccc ttttcacttt gctgctccaa
     5461 tatcatcgtc tgataatagg ctgtatctcg ctgagcatat acttgccacg aatccgaaaa
     5521 atgttcctca aaaaaatctg ccatctcttc tgcgtcccac aatcccgcat ctgaaaattc
     5581 aagcgttcct tctttctgcg gctcactttc acgatttcta tcatctgctg tctcatcttt
     5641 tttgacttct tgcatgcatt ggtcataaat atatctaaaa tcataaggtc tgtaaatcgc
     5701 ttctgccgca ggcattaaaa atgtaaacgg cactttttcc gaatgcatcc gttccaaaac
     5761 agtatttagc agcgcccgca tatatcctcg actgcgatat tcttttttcg ttgcaacagc
     5821 gacaatatat tctgacggaa agcttttatc ctccagccgg atccgatatg gattcaactg
     5881 aagcattgaa caaattctcc catcctcttc cacaacgtaa atttgatttt ttgccgcttt
     5941 aatatagtaa taatagtcta ggaatgcctt cgtatcttcc ggaaatattt cttcccacaa
     6001 ttttcgtgtc gcaatatgtt cgctctgctc taatttacgg atgatcattt actcgttcct
     6061 cccgatgcgc gaacaacatc ctccgcacat cccacaatcc gaagagacat ccgtactgct
     6121 gtaatttgta tatttctcga tcgcacaaat cgcctgagaa gaaatccctt ctccgtcccc
     6181 tgtaaaccca agtccttcct ccgttgtcgc tttgacattg acctgatcga tctcaataca
     6241 gagtgcatct gcaatatttt gacgcattgt atcaatatac gggcgcatct tcggacgttg
     6301 agcaatgatc gtcgcatcga tattttcaat catataccta ttctcatcca aaagccctcc
     6361 tacatgtttc agaagccgaa tgctggaaat ccctttatat tccggatcgg tatccggaaa
     6421 atgtttcccg atatcaccta atgccgcagc tcctaaaagc gcatccataa tcgcatgcac
     6481 gagcacatct gcatccgaat ggcccaaaag acctttttca tacggaattt ccacgcctcc
     6541 caaaatcatc tttctatctt ccgccaatct gtgaacgtca tatcccatac ctactcgcat
     6601 gctcatctct cctcataatc attttctatc tctgtttccg tctcttcaat aaattcttcc
     6661 attgccaatt caggctcctg attttttcca ccttctttta tcatttcatc ttctgcatct
     6721 tcctcgtttt tgtcatttgg cttttctgcc tcttttttca ctttatcatc cgattgttct
     6781 gtctccgatc ctgccttata gcctgatttt tccgtttctc cttctgcttt atcatctaac
     6841 ttttctgcct ctttttcgat gttattaccg gactgtgctg tttccttatc tgttttcccc
     6901 tgctctgctt cttttttcat ttcctcattc ggaccaattt ccggcaaaac attttttctt
     6961 ctttttcgat ccgcctgatc ttttcccgca tcgaatgcct ctttatcttc ctcttcaatc
     7021 ctatgatctt cttttttcag atcaaacatt tttttcacat aataaatagc ataggctaaa
     7081 ccgatcaata taaagattac agacatcaca cacataaaaa ccatattatt cggtttttca
     7141 tttaccattt caatcaacat ccttatccca agccaagcta aatatcctcc taaaacaatc
     7201 cgaaaggtat accatgcctt tttattcatt ttcctcttcc tccgtaatta ttatgtgctt
     7261 tattgtaaca tatttttctt ttttcttaaa gcaactcgtg acatactccc accgcctaaa
     7321 gaggtgagga cttctcacga atctgtggaa attctttttc agaagtctgt ggctgcactt
     7381 ttatgaacat ttataaattt actgatttca gacttcggat gtgctttaaa aagaatatga
     7441 tctttcatca tttcgactgc ttatataata tcatatcttt gtgtacttat gaataatata
     7501 taactcaatt ccttcccctc tttagaagta gaggattcct tgtaaaaggt aggttaaaaa
     7561 acatatacct gcaccttttt aaaattaatg gaaaacagat ttccgaaaat gaacatagaa
     7621 actggatttt actcttacat ttaccttaaa aattaaaaaa tttaaaaata aaaatttttt
     7681 taaaaaaagt gttgacattt gatgaaaaat aatagtataa tagcgaagcg ggttacggaa
     7741 aagcttctga aacccaaaac aatatggggt cttagctcag ctgggagagc atctgcctta
     7801 caagcagagg gtcataggtt cgagccctat aggccccata acttacacat attcggaaca
     7861 ttttgttcaa gttatggcgg aatagctcag ttggctagag cacacggttc atacccgtgg
     7921 tgtcgctggt tcaaatccag tttccgctac taataaaaac tccaagatat tttcttggag
     7981 tttttattat attcttttct ttttcatctg ttgttatccc cctcacccct ctgctttttc
     8041 tcacttttga acataagtct gcccgcgcct gctctgacaa tattaaatga tcatacaaaa
     8101 gcagagcata cactcgcata tgccctgctt ttgcattact gactttttac ttaaaatatt
     8161 ttactccgga ttcaaaaatc ttaagatcct gctctccata aatattaatc gcaacagaat
     8221 taccccttct ttcagagtga gccatttttc caagcactcg accgtccgga cttgtgattc
     8281 cttcaatcgc gtgataagat ccattaacat tccattcttc atccatcgta atatgtcctt
     8341 caggactaca atattgtgtc gccacctgac cattcgcaaa caacttgtca atccattctt
     8401 cactcgcaac aaatcgtcct tctccatgag acgcaggatt tgtatatacg cctccgagtt
     8461 ttgcctctgc aagccatgga gatttatttg taaccacttt tgtataaacc atcttcgata
     8521 tatgacgtcc tattgtgtta tacgtcaaag tcggagaatc atcggtctgt ccgacgatct
     8581 gtccatatgg aacgagtcca agcttgatca aagcctgaaa accattgcat acgcctaatg
     8641 caagtccgtc gcgttcattt agcaatttat ctaccgcttc tttaatcttt gcgtttctaa
     8701 acgctgttgc aaagaacttt gccgatccat caggttcatc tcccgcacta aatccaccgg
     8761 ggaacataat aatctgcgct ttagaaatcg ctttttcaaa ctcttccacc gaagaacgga
     8821 tgtcttctgc atctaagttt ctaaacacct tcgtaatcac atttgcaccg gctctctcaa
     8881 aagccctcgc actgtcatac tcacagtttg tacccgggaa taccggaata aacaccgtag
     8941 gctgtccgat cttatggctg cacacatgta cggttgaagc atcgtataac ttttcctcta
     9001 ccggtatatc cgaactttcc gctgattttg ttgcaaatac tttttcaagc ggttttgtcc
     9061 atgcatccag cgcatcatcc ataccgatct tcacatcgcc atatgcgaac gccttttcat
     9121 ttgtcacttc accgatcaaa gtatattctc ccacaagctc ttcaaacgtg tctgctttaa
     9181 cctctgcgac gatatcgcca aatgcaggag caaaaatcgc atctttatcc aaactgcttt
     9241 caagacatac acccatttga tttccgaatg ccattttgct gactgccgaa acaacaccat
     9301 gtctgtcaag cgcataagcc gaaacaactc ttccatttct gatatcttct gtaaacttgc
     9361 tatactgttc cataatccgg ctataaacag gaaggtcata ctcatcacga gccgctttca
     9421 gccacacaag cttatttcct gcctctttca attccggcgt aataatttct ttttctactg
     9481 ccatatctac agcaaacgaa accaaagtcg gcggaacatc tatatcctga aatgttccgg
     9541 acatactgtc ttttcctccg atcgacggaa gtccgaatcc gatctgtgcc gcataagctc
     9601 caagaagcgc tgcaaaaggc tggctccaac gttttggatc ttctgacatt cttctgaaat
     9661 attcctgaaa cgtaaatctg atcttactgt aatctccacc tgccgcaact atcttggcaa
     9721 ccgattcaac aaccgcgtaa gctgcgccat gatacggact ccaacttgaa agatacgggt
     9781 caaatccata actcatcata gacacactgt cagttgttcc gttttgcacg ggaacctttg
     9841 ctaccatggc ctgtgtctct gtcatctgat attttccacc gtgaggcata aagaccgagc
     9901 ctgcgccgat tgaaccgtca aacatctcaa caagtccttt ctgagagcag acattcaaat
     9961 cgcttaatac atccagccat ttttccttca catcacttac tgctgtttta acaaataaac
    10021 tttcttcttg acgaggtatt tccacttcta ccgccgtttc ctgatgcgct ccattcatat
    10081 ccaaaaacgc tcgggaaaga ttgacaatct cttttcctct ccatacaaga acaagtcttg
    10141 gctcttctgt aacaaccgct acttccgtcg cttccagatt ctcttctttc gcataggaca
    10201 taaacttctc tacatctttt ggatctacaa caacagccat tctttcctgg gattccgaaa
    10261 ttgcaatttc tgttccgtcg agacctgcat atttcttcgg cactttatca agttcaaccc
    10321 ggagaccatc tgccagttcg ccgattgcca cagacactcc tcccgcaccg aaatcgttgc
    10381 attttttaat aaggcagctt acctcttcac ggcggaataa tctttgaatc ttacgctctg
    10441 taggcgggtt tcctttctga acttccgcac cgcatgtttc gatcgactct tctgtatgca
    10501 ctttagacga accggtcgca ccgccacagc cgtcacgtcc tgtacgtccg ccaagcaaaa
    10561 taataatatc tcccggatca gaattctccc ggattaccgc ccttctcgga gccgctccca
    10621 ataccgctcc tatttccatt ctttttgcaa cataatcagg atgataaatc tcttttacaa
    10681 gacctgtagc caatcctatc tgatttccgt aagaactgta gcctgaagcc gctcccctaa
    10741 caagtttttt ctgcggaagc tttcctttta atgtttcttc tacagatact gtcggatctg
    10801 cagctcctgt cactctcatt gcctgatata catatgtacg acctgacagc ggatctcgaa
    10861 tcgcaccgcc aagacatgta gccgcgccgc caaacggttc gatttccgtc ggatgattat
    10921 gcgtttcatt tttaaagttg atcagccact cttcttctac gccgtccact ttgatcggaa
    10981 ccacaatact gcatgcattg atttcatctg atttttcctg atcctgaagt tttccttctt
    11041 ttttcaatct gcgcattgcc atcaacgcca gatccatcag gcagacaaac ttgtcctctc
    11101 taccagcgaa aatctcacta tgatctttca gatatgcttt atatgtatcc tcaatcggtt
    11161 tcttataaaa accttcatta aaagatactt ccttcaactc tgtcgaaaat gttgtatgac
    11221 ggcaatgatc ggaccagtat gtatcaagaa ctcggatctc cgtcatcgtc ggatctctat
    11281 gctcttcatc tttataataa ttgcgaatat gaagaaaatc tttaaaagtc attgccagcc
    11341 ccaatgaact ataaagcttt tctagttcgt cttccttcat ttctttgaat ccttcaaata
    11401 cgatcacatc ttccggatca tcaaaaaccg tcacgagtgt ttccggtttt tcttctccgg
    11461 cttctcttga atcaaccgga ttgatacaat gattctttat cgccgcaaat tcttcttccg
    11521 aaatcgtacc ttcgatcaca tacgtcgttg ccgtatggat caccggtgtt tcatcctctt
    11581 taataaatct tacacactgt tctgccgaat cagctctttg atcaaactgt cccggaagat
    11641 actctaccga aaagctgtga tccgcatcag acatattaaa tgtctcttta taaagaatat
    11701 ctacaggcgg ttctgcaaat accccctgac atgccttttc aaaagtttca tctgaaatat
    11761 tttccacatc atatcgaatc agcacacgca ctcccgtcac atctgtaata ccgagataat
    11821 ggcggatttc atgacccagc tccttcgcag caacagcaaa ctccggcttc ttctctacaa
    11881 atacacgttt cacattactc atatgttgtc tcctccattt ttaactgaaa acctctgttt
    11941 gtatcgtacc acacttcaca tcattagtat aattaatata actaaacttt ctaataagca
    12001 atactaatca tttttctttt ttcctgtttt ttcttttact tttcgactgt tttctctcca
    12061 tctcatataa agact
//
LOCUS       DBMDXF010000042        11931 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1535_length_11931_cov_1.727134, whole genome shotgun sequence.
ACCESSION   DBMDXF010000042 DBMDXF010000000
VERSION     DBMDXF010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1535_length_11931_cov_1.727134"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            83..679
                     /locus_tag="ACZPD4_02965"
     CDS             83..679
                     /locus_tag="ACZPD4_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HIR1378069.1"
                     /translation="MDLKKAQEYIKALKNGEKFPKKNMFLIMFLLGALLFVIVLPTGS
                     SAPTSEKGGLDENKRKENKNSQTGEYETYIEEKTSSVLSQVKGAGKVTVMVTLKSNGQ
                     KLIEKDHSGSTQTQEETSDGNVRKDSESTSEKISVYEQTADGASVPYVSKELTPEVEG
                     VIVIADGGGDAVVARNLTEAVQALFGVEAHKIKIMKRG"
     gene            703..1314
                     /locus_tag="ACZPD4_02970"
     CDS             703..1314
                     /locus_tag="ACZPD4_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR1378070.1"
                     /translation="MKRVFKKNQIIIAALAVMIAVAGYLNYSGKIFHEKDGGAEEANA
                     DLANQELLDISEEDIETVGDEIASNDSDVEGTPGEAVLTNGSAETTVAQAKVTREQVR
                     AQNKETLQAIIDNTNLSESEKQEAVSQLVQMTQIAEQEAAVETMMASKGFSEAVVSID
                     ADSADIVVKAEELTDANRAQIEDIVTRKTKIAPQNIVITPIRE"
     gene            1493..3085
                     /locus_tag="ACZPD4_02975"
     CDS             1493..3085
                     /locus_tag="ACZPD4_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR1378071.1"
                     /translation="MSEITNEIKKRRTFAIISHPDAGKTTLTEKFLLYGGVINQAGSV
                     KGKATSKHAVSDWMEIEKERGISVTSSVLQFNYDGYCINILDTPGHQDFSEDTYRTLM
                     AADSAVMVIDASKGVEAQTRKLFKVCAMRHIPIFTFINKMDRDAKDTFDLLDDIEKEL
                     GIPTCPVNWPIGSGKAFKGVYDRQKKEVELFSDTRKGTATGEVKVVAMNNPEIDWLIG
                     DEAKTTLMEEIELLDGASAEFDRELVDKGELSPVFFGSALTNFGVETFLRHFLSMTTS
                     PLPRMSDHGAIDPMKEKEFSAFVFKIQANMNKAHRDRIAFMRICSGEFDAGMNVYHVQ
                     GKKDVRLSQPQQMIASERKIIDKAYGGDIIGVFDPGIFSIGDTLTTSKEKFAYEGIPT
                     FAPEHFARVRQVDTMKRKQFVKGINQIAQEGAIQIFQEYNTGMEEIIVGVVGVLQFDV
                     LKYRLKNEYNVEILLENLPYEYIRWIESADTDLDRISGTSDMKKIKDLKDRPLLLFIH
                     EWSIRMTLERNDGLVLSEFGRS"
     gene            3195..3578
                     /locus_tag="ACZPD4_02980"
     CDS             3195..3578
                     /locus_tag="ACZPD4_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1378072.1"
                     /translation="MAKDERNAYTIQNDASLGEVKIADEVVAIIAALAATEVDGVASM
                     AGNITNEVIGKLGIKNLSKGVKVDVLEGVVTVSLALNLKYDYSIMEVTKKVQEKVKNA
                     VENMTGLEVADVNIKVAGVEMEKQN"
     gene            3766..4173
                     /gene="nusB"
                     /locus_tag="ACZPD4_02985"
     CDS             3766..4173
                     /gene="nusB"
                     /locus_tag="ACZPD4_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1378073.1"
                     /translation="MVRTELREHIFKMLFQIEFNDSVDMPENLNYYFETLEDAKEKDK
                     QYIQNKYEAILSHIEEIDGLLNENATGWKTTRMNKVDLSILRLALYEIKWDEDVPAKV
                     AINEAVELAKRFGGDNSSSFVNGVLAKFAKQEL"
     gene            4175..5383
                     /gene="xseA"
                     /locus_tag="ACZPD4_02990"
     CDS             4175..5383
                     /gene="xseA"
                     /locus_tag="ACZPD4_02990"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1378074.1"
                     /translation="MNGIYTVKQVNSYIKNMFTQDFMLNRIYVKGEISNCKYHPSGHI
                     YFSLKDESGTIACVMFAGSRAGLSFTLKNGQRVIAFGSVSVYERDGKYQLYAREIKLD
                     GAGELYEKFEALKRELCEMGMFSDEYKQRIPAYVKTVGIVTAQNGAAVRDIINVAKRR
                     NPYVQLILYPALVQGEGAVKSIIRGIRMLEQQGVDVMIVGRGGGSIEELWAFNEEEVA
                     RAIFECSVPVISAVGHETDVTIADFVADLRAPTPSAAAELAVYEIRECKKTMSAYEDR
                     IVRAALSQIGLFRTKAERFSMRLKLLHPRHKLYEHRQFTAEMENRIRDIMERRLTQEK
                     HRLALNAERMKRVSPLLKLSSGYSYIRSEEGKNIRSTAQVKRGEKLEIFVSDGKIEAE
                     VHNTETMIHG"
     gene            5406..5618
                     /gene="xseB"
                     /locus_tag="ACZPD4_02995"
     CDS             5406..5618
                     /gene="xseB"
                     /locus_tag="ACZPD4_02995"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1378075.1"
                     /translation="MSEKKKENLEEMFKDLEELIGKMENEEITLEQTFDLYNNGMELL
                     KKCNLSIDEVEKKVLVLDENGETDEF"
     gene            5608..6498
                     /locus_tag="ACZPD4_03000"
     CDS             5608..6498
                     /locus_tag="ACZPD4_03000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1378076.1"
                     /translation="MNFKEDCRKRTEKIEEILRKYLPDQTGHQQIIEEAMEYSLMAGG
                     KRLRPMLMKETFDLFGGKGGVIEPFMAAIEMIHTYSLVHDDLPAMDNDDYRRGRKTTH
                     VVYGEDMGILTGDALLNYAFETACSAFETDPQNSILIGRALKILAGKAGIYGMIGGQV
                     VDVRSAGCKISGELLAFIYRLKTGALIEASVMIGAVLAGAKEEEIGKMEEIAGKIGLA
                     FQIQDDILDVTSTTEILGKPVHSDEKNEKTTYVTWKGLEKAKEEAARLTEEAVEELRS
                     IGKECGFMEELLRRLVYREK"
     gene            6516..8375
                     /gene="dxs"
                     /locus_tag="ACZPD4_03005"
     CDS             6516..8375
                     /gene="dxs"
                     /locus_tag="ACZPD4_03005"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1378077.1"
                     /translation="MIIEMIQKENDIKKLDHTQLKELAEEIRGFLLDKISCTGGHLAS
                     NLGVVELTMALHLTLDFPQDKLIWDVGHQSYTHKLLTGRKDGFDDLRKYGGMSGFPKR
                     KESECDAFDTGHSSTSISAGLGYVAARELKGEKYNVVSVIGDGAMTGGMAFEALNNAS
                     RLKSNFIIVLNDNNMSISENVGGVSKYLGCLRTAQAYTDLKKGVEDTLKKIPGTGDRI
                     VSRLKKTKSGIKQLFVPGMFFEDMDITYLGPVDGHDVKEMVRVFNEAKRVDHAVLVHV
                     LTKKGKGYLPAEENPAKFHGTGPFHLETGDAKESKGADTYTEVFSKVLLDIAKKDERI
                     VAITAAMADGTGLTPFAKHFPNRFFDVGIAEEHAMTFAAGLAAGGMKPIFAVYSSFLQ
                     RAYDQTLHDVCLQNLPVVIAVDRAGLVGSDGETHQGVFDLSFLSMIPNMTIISPKNRW
                     EMADMIRFAADFPYPIAVRYPRGEAYEGMKQFRSPICYGKSELLYEEEKIVIIFVGHM
                     AELADQVRKGLKEKGYCCSLVNARFVKPLDTELLERLAENHDLYVTIEENMLTGGFGE
                     QVLDYISGAGINVRVKNFGIADDYVEHGNVDLLRREVGLDKDTIVKQIIDEYV"
     gene            8392..9210
                     /locus_tag="ACZPD4_03010"
     CDS             8392..9210
                     /locus_tag="ACZPD4_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1378078.1"
                     /translation="MKERLDVLLVKRNLAESREKAKAIIMSGNVFVEGQREDKAGSTF
                     PEEVSIEVRGHTLPYVSRGGLKLEKAIANFDVSVKDKVCTDVGSSTGGFTDCMLQNGA
                     KKVFAIDVGRGQLDWKLRNDPRVICMEKTNIRYVTPEDLGEEIDFSSIDVSFISLTKV
                     LEPIRNYLAKDGEIVALIKPQFEAGREKVGKKGVVREKSTHREVIQKIMDYAMSIGFD
                     LCALDFSPIKGPEGNIEYLIHLKKSENEAGENLGGLDPVIVSDRAFETLAKQHA"
     gene            9255..10088
                     /locus_tag="ACZPD4_03015"
     CDS             9255..10088
                     /locus_tag="ACZPD4_03015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1378079.1"
                     /translation="MDCFYIITNKLKDKDYAITSQIEQYIQSSGKKCFLSEKDSEGHI
                     IPGTVPKEAECGLVLGGDGTLIRAVRDLGERSLPLLGINLGTLGYLTDVDLEDFESAL
                     DHLFSETPVIEERMMLEGSFRNSRKDMAMNDIVIAREGKVRIVSFHIYVNGALLNTYH
                     ADGVIISTPTGSTGYNLSAGGPIVEPTAQMIVITPICSHALNTSSIVLSADDLIEVEV
                     CEGRYGRQEQVSLCFDGAEQTTLVTGERVCIKRSSHTAKLIKLSKESFMKTMRKKMKG
                     N"
     gene            10093..10551
                     /locus_tag="ACZPD4_03020"
     CDS             10093..10551
                     /locus_tag="ACZPD4_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR1378080.1"
                     /translation="MKVSRHAKIIELISQYDIETQEELAEYLNNAGFKVTQATVSRDI
                     RDLKLSKVSTGSGHQKYILHRIEEPGMSEKYIRVLKDGYVAMDMAQNILVIKTVSGMA
                     MAVAAAVDAMKWHEVVGCIAGDDTIMCAIRTVEDTAAVMDKIRKIVSREV"
     gene            10561..>11931
                     /locus_tag="ACZPD4_03025"
     CDS             10561..>11931
                     /locus_tag="ACZPD4_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1378081.1"
                     /translation="MLQNLHVKNIALIDETEVDFGQGLNILTGETGAGKSILLGSVGL
                     ALGGKYSSDLLRNGAENGLVELTFSVDDGQIRQKLEEMDIIPDDGMVTLTRKFTGSRS
                     ISRINGETVNTGKLKEAAELLIDIHGQHDNKTLLQRKNHLVLLDLFGRKEIAPIKSEM
                     SKCYKEYRAICKKEEETSLNDEERRRELSLYEFEVHEIEEAALRENEDEELEETYRRM
                     TESRKITDAVAQTYRYTCEDSSANAGDCLTRAIRAFREAAEFDEEGDRLCALLMDVDS
                     LLNDFNRELSEYASKFEFSEEEFKETEERLNLINHLKAKYGSSVSDVLAYCESKRHRI
                     EQLNDYDAYIKQIEEEKEKALKKVERVTKKLHDVRNRYKTVFADEICIHLKELNFLDT
                     RFEIHLKDAGQFSANGKDEAEFYLALNPGEPVKPLENIASGGELSRIMLAIKTVLADA
                     EDTPTLI"
BASE COUNT         3964 a         1847 c         3134 g         2986 t
ORIGIN      
        1 aattatatag aagaaaataa agaggaagaa catacaggaa caaaaaaagc ggatacagat
       61 gccggaaagg agtcgataaa agatggattt gaaaaaggct caagagtata ttaaggcatt
      121 aaaaaacggg gaaaagtttc cgaagaaaaa tatgtttctc attatgtttt tgcttggtgc
      181 gctgctgttt gttattgttc ttccgaccgg ttcctctgcc ccgacttcgg aaaaaggcgg
      241 attagatgaa aataaaagaa aagaaaataa aaattcacaa acgggagaat atgaaacata
      301 catagaagaa aaaacatctt ccgtgctgtc gcaggtgaaa ggagcaggaa aagtaaccgt
      361 gatggtgacg cttaaatcaa acggacagaa attgattgaa aaagatcatt caggaagtac
      421 gcagacccaa gaagagacat ccgacggcaa tgttcggaaa gattcggaaa gtacgtcgga
      481 gaaaatcagc gtatatgagc agacggcaga cggagcatct gttccttatg taagcaaaga
      541 actgacgccg gaagttgaag gggtgattgt gattgcggac ggaggaggtg atgcagttgt
      601 tgcccgtaat cttacagaag cggtacaggc attatttggt gtggaagcgc ataagataaa
      661 aataatgaaa cggggatgat acataaaaag gaggcttgtg aagtgaaacg agtatttaag
      721 aaaaatcaga ttatcatagc tgcgttggcg gtgatgatcg cagtggcggg atatttgaat
      781 tattccggaa aaatatttca tgaaaaagac gggggagctg aggaagcaaa tgcagatctt
      841 gcaaatcagg agctgctcga tatttcggaa gaggatatag agacggtagg agacgagatt
      901 gcaagtaatg attccgatgt agagggaaca ccgggggaag ctgtactgac gaatggaagc
      961 gcagaaacga cagttgcaca ggcaaaggta acacgggagc aggtaagagc tcagaataaa
     1021 gagacgcttc aggcaattat agataataca aatttaagtg agtcggaaaa acaagaagcg
     1081 gtatcacagc ttgtacagat gactcagatt gcagagcagg aagcggcagt ggagacaatg
     1141 atggcttcaa aaggattttc ggaagcggtt gtaagtattg acgcagactc tgcggatatc
     1201 gtagtaaaag cagaagaact tacagatgcg aatcgagctc aaatcgagga tattgtgaca
     1261 agaaagacaa aaattgcacc gcaaaatatt gtgatcaccc cgattcgtga ataaaaagta
     1321 tctgctgtat gtgaaaatga aaaattcgat tgtatctgaa tgtttgcctg aatcccgtgg
     1381 ttgaagtatc ttttgagata tgataaacta aattggtatg tccggaagat gttccgggaa
     1441 aaataaaaga acgaacagat gcattttata agcattagga gaaaagaaac atatgtcaga
     1501 gataacgaat gaaataaaaa agagaagaac atttgccatc atttctcatc ccgatgcggg
     1561 aaagacaacg ctgacggaaa aatttctatt atatggaggg gtgatcaatc aggcaggttc
     1621 tgtcaaggga aaagcgacat ccaaacatgc agtttcagac tggatggaaa tagaaaaaga
     1681 aagaggtatt tcggttactt cttctgtatt gcagtttaat tacgatggat actgtattaa
     1741 tatccttgat acgccggggc atcaggattt ctcggaagat acttacagaa cactgatggc
     1801 ggcagattcg gcggttatgg tgattgatgc gtcgaagggt gtggaggctc agacgcgaaa
     1861 actgtttaaa gtatgtgcga tgcgtcatat tccgattttt acctttatca ataagatgga
     1921 tcgtgacgca aaagatacgt tcgatctttt ggatgatatc gaaaaggagc tcggcattcc
     1981 gacttgtccg gtaaactggc cgatcggctc gggaaaagca tttaaaggcg tgtatgacag
     2041 acagaaaaag gaagtagagc ttttctcgga tacgaggaaa ggaacagcga caggtgaagt
     2101 gaaagttgta gctatgaaca atccggagat tgactggctg atcggagatg aagcaaagac
     2161 aactctgatg gaagaaatcg aacttttgga cggcgcttcg gcagaatttg acagagagct
     2221 tgtcgataaa ggcgagctct ctccggtgtt cttcggttct gcacttacta acttcggagt
     2281 ggaaacgttt ttaagacatt ttctttccat gacgacgtcc cctttgccga ggatgtcaga
     2341 tcatggtgcg atcgatccga tgaaagaaaa agaattttcg gcctttgttt ttaaaattca
     2401 ggcaaatatg aataaagctc atagggacag aattgcgttt atgcgtattt gttcgggtga
     2461 gtttgatgcc gggatgaacg tatatcatgt tcaggggaaa aaagacgtaa ggctttccca
     2521 gccgcagcaa atgattgcca gtgaaaggaa aattatagac aaagcatatg ggggagatat
     2581 tatcggtgta tttgatccgg gaattttttc gataggcgat acactgacaa cgtcaaaaga
     2641 aaaatttgct tatgaaggaa ttccgacatt tgcgcctgag cattttgcaa gagtgcgtca
     2701 ggttgacacg atgaaaagga aacagtttgt aaaaggaatc aatcaaattg cacaggaagg
     2761 agcaattcag atctttcagg aatataacac aggaatggaa gaaatcattg tcggcgttgt
     2821 cggcgttttg cagtttgatg tattgaaata ccgtttgaaa aatgaatata atgtagagat
     2881 cctcctcgaa aaccttccgt acgaatatat ccgatggatc gagagtgcgg atacggatct
     2941 tgaccgtata agcggaacgt cggatatgaa gaaaataaaa gatttgaaag accggccgtt
     3001 acttttgttc atccatgagt ggagcattag aatgacgttg gaaagaaatg acggacttgt
     3061 tctttcggag tttggaagat cttaaaaatc ggattcaaat acagagttct cttggcggtg
     3121 aagtttttct ttgcaaacgg atggagattt gttataataa gaaaagagat gatacaaaac
     3181 gggaggtttt gaatatggct aaggacgaaa gaaatgctta tacaatacaa aatgatgcga
     3241 gtctcggcga agttaagatc gcggatgaag ttgtagcgat cattgcggct ctggctgcta
     3301 cggaagtgga tggcgttgct tccatggccg gaaatattac gaatgaagtg attggaaagc
     3361 ttggaatcaa gaacctgtca aaaggcgtaa aggtagatgt attagagggt gtcgttacag
     3421 tatctctggc attaaacctg aaatatgatt acagtattat ggaagttacg aaaaaagttc
     3481 aggaaaaggt gaaaaatgcg gttgagaata tgacggggct tgaggttgcg gatgtcaata
     3541 ttaaggttgc cggagtagag atggagaaac agaactgatc tgctttcgta ttgcaatatg
     3601 tgacaaggcg atccgcccct gacattgata ctaagggatg tcgcaagaca tcctttttca
     3661 atattgcgcg gcaataagat acaagcgggt acgagcagga caggaagatg gcaaaaggcc
     3721 gtgtgcgccg acagaagatg attatgacaa tagagaggaa ttactatggt aagaacggaa
     3781 cttcgggaac atatatttaa aatgcttttt cagatcgagt ttaatgattc tgttgatatg
     3841 ccggagaatt tgaactacta ttttgagacg cttgaagatg caaaagaaaa agacaaacag
     3901 tatatacaaa ataaatatga agcgatttta tcacacatag aagaaatcga cggactttta
     3961 aatgagaacg cgacaggctg gaaaacgaca agaatgaaca aagtcgatct gtcaatttta
     4021 cggttggcgc tttacgagat aaaatgggat gaggacgttc cggcaaaagt tgcgatcaac
     4081 gaggctgttg agcttgcgaa acgtttcgga ggagataata gctcgtcgtt tgtaaatggt
     4141 gtgcttgcta aatttgcaaa acaggagtta taaaatgaac ggcatttata cagtgaagca
     4201 ggttaattct tatataaaaa atatgtttac acaggatttc atgctgaacc gtatctatgt
     4261 aaagggagaa atttccaact gtaaatatca cccatcagga catatttatt tttctttgaa
     4321 agatgagtcc ggcacgattg cctgtgttat gtttgcaggc tcaagggcgg ggctttcttt
     4381 tacattgaaa aacggccagc gggtcatcgc attcggttca gtaagcgtat atgaaagaga
     4441 tggcaaatat cagctttatg caagagaaat caagctggac ggagcaggag aactctatga
     4501 aaagttcgag gcgcttaagc gggaactttg tgaaatgggg atgttttcag acgaatataa
     4561 gcagcgaatt cctgcctatg taaaaacagt tgggattgtg acggcgcaaa acggagcggc
     4621 ggtaagagat ataatcaatg ttgcaaaacg tagaaatcca tatgttcagc tcattcttta
     4681 tccggccctt gttcagggtg aaggagcggt gaagagcatt ataagaggaa tacggatgct
     4741 agaacaacaa ggtgtggatg tcatgatcgt tggcagaggc ggcggaagta ttgaggagtt
     4801 gtgggcgttt aacgaagaag aggttgcacg tgcaattttt gaatgttccg ttccggtcat
     4861 atctgcggta ggtcatgaaa cagatgttac aatcgcagat tttgttgcgg atttacgagc
     4921 tccgactccc tcagcagccg ccgaattggc agtgtacgag atccgcgaat gtaaaaaaac
     4981 gatgtctgcg tatgaagaca gaattgttcg ggctgccttg tcacagatcg gtcttttcag
     5041 gacgaaagcg gagcgtttct caatgaggct gaaactgctt catccacggc acaaattata
     5101 tgagcacagg caatttacgg cagagatgga aaacaggatt cgcgatatta tggaacgaag
     5161 actgacgcag gaaaaacatc gtctggcctt gaatgccgaa cgaatgaaaa gagtgtctcc
     5221 tcttttaaaa ctgagcagcg gatactcgta tataagatcc gaagaaggaa aaaatatacg
     5281 atcgacagct caggtgaaac ggggagaaaa attggaaatc tttgtaagcg acggaaagat
     5341 cgaagcggag gttcataata cggaaacaat gattcacgga taaaaacgga tcggcggagg
     5401 ttaaagtgtc agagaaaaag aaagaaaatc ttgaggagat gttcaaagat ttggaagaac
     5461 tgatcggaaa gatggaaaat gaagaaatca cgttagaaca aacatttgat ctatataata
     5521 acggaatgga acttcttaaa aaatgtaact tatccataga cgaggtagaa aaaaaggttc
     5581 tcgttcttga tgaaaatgga gagacagatg aattttaaag aagactgtag aaaacgaaca
     5641 gaaaaaatag aagagatttt acgaaaatac ctgcctgatc agacaggaca tcagcagatc
     5701 atagaagaag cgatggaata tagtctgatg gcaggtggaa aacgccttcg tccgatgctc
     5761 atgaaagaga cgtttgactt gtttggcgga aaaggcgggg tcatcgagcc gtttatggcc
     5821 gctatcgaga tgatccatac atattctctt gttcatgatg accttccggc catggataat
     5881 gatgattatc gaagaggacg gaagacgact catgtcgtat acggagaaga tatgggaatt
     5941 ttgacagggg acgcactttt gaattacgca tttgagactg catgcagcgc ctttgaaaca
     6001 gatcctcaaa acagcatcct gatcggaaga gctctgaaaa ttcttgcagg caaagccgga
     6061 atttacggaa tgatcggagg tcaggtggtc gatgtaagat ctgcaggatg caaaatatca
     6121 ggagaacttc ttgcgtttat ttatagattg aaaacaggag cgcttattga ggcgtctgta
     6181 atgatcggag ctgttcttgc aggggcaaaa gaagaggaaa tcggaaaaat ggaagaaatc
     6241 gccgggaaaa taggactggc attccaaata caagacgata ttcttgatgt gacgagtacg
     6301 acagaaatac tcggaaagcc ggtgcacagc gatgaaaaaa acgaaaaaac gacttacgtt
     6361 acttggaagg ggcttgaaaa ggcaaaagaa gaggctgctc gtctgacgga agaggcggta
     6421 gaagaacttc gttctatcgg aaaagagtgc ggatttatgg aggaactgct ccgaagactt
     6481 gtatacagag aaaaatagaa ctaggaggat caatcatgat aattgaaatg atccagaaag
     6541 agaacgatat aaaaaaactt gatcatacac agctaaaaga acttgcagaa gagatccggg
     6601 gatttcttct tgataagatc agttgtacag gaggacatct ggcttcaaat ctgggtgttg
     6661 tagaattgac gatggcgctt catcttacac tggattttcc gcaggataaa ttgatctggg
     6721 atgtaggtca tcaatcttat acgcataaac ttcttacagg aagaaaagat ggttttgacg
     6781 atctgcgtaa atatggcggc atgagtggat ttccgaaacg aaaagaaagt gaatgtgatg
     6841 cttttgatac agggcatagt tctacatcta tttcggcagg tctggggtat gttgcggcaa
     6901 gagaactgaa aggggaaaaa tacaatgtcg tttcagtgat cggagacgga gcaatgaccg
     6961 ggggaatggc atttgaggca ttgaataatg cttcccgttt gaaaagtaat tttattatcg
     7021 tgctgaatga taataatatg tcaatttctg aaaatgtcgg cggagtatcg aaatatttgg
     7081 gatgcctgcg gacagcacag gcgtatactg atctgaaaaa gggtgttgaa gatactttaa
     7141 aaaaaatacc ggggacaggc gatcggatcg tcagcaggct gaaaaagaca aaaagtggga
     7201 ttaaacagtt atttgttccg ggaatgtttt ttgaagatat ggatattaca tatttaggac
     7261 ctgtggacgg acatgatgtt aaagagatgg tgcgtgtgtt caatgaggca aaaagggtgg
     7321 atcatgccgt tttagtacat gtacttacga aaaaaggaaa agggtatctt ccggcagagg
     7381 aaaatccggc aaaatttcat gggacaggtc cgttccatct tgagaccgga gatgcgaaag
     7441 aatcaaaagg ggcggatact tatacggaag tattttctaa agtgctgctt gatattgcaa
     7501 aaaaagacga gcgtatcgtt gcgattacgg ctgcgatggc ggacggaaca ggtcttactc
     7561 cgtttgcaaa gcatttccca aatcgttttt ttgatgtggg gatcgcggaa gaacatgcga
     7621 tgacatttgc ggcaggtctt gcggcggggg gaatgaaacc gatatttgca gtatattctt
     7681 cgtttttgca aagagcgtat gaccagacgc ttcatgatgt ctgtctgcaa aatcttccgg
     7741 ttgtgatcgc ggtagacagg gcaggacttg tcggaagtga cggtgaaaca catcaggggg
     7801 tgtttgattt gtcattttta tcaatgatcc cgaatatgac gatcatttct ccgaaaaaca
     7861 gatgggaaat ggcagatatg atacggtttg cggcagattt tccatatccg atcgcagtgc
     7921 ggtatccaag aggcgaggca tatgaaggaa tgaaacaatt ccgcagtccg atctgttacg
     7981 ggaaaagtga actgctgtat gaagaagaaa agattgtgat catatttgta ggtcatatgg
     8041 cagaacttgc agatcaggtg agaaaaggcc tgaaagaaaa aggatattgc tgttctcttg
     8101 taaatgcaag gtttgtaaaa cctcttgata cagaattgct ggaaagatta gcggagaatc
     8161 acgatctgta tgtaacgatt gaagaaaaca tgctgacagg cggattcggg gaacaggttc
     8221 ttgattatat ttcaggagca ggtattaatg tacgcgtgaa aaatttcggg atagccgatg
     8281 actatgtaga acatggaaat gtagatctgc tccgcaggga agtaggtctt gataaagata
     8341 cgatcgtaaa gcagataatt gatgaatatg tgtaactgaa aggaaaaaaa tatgaaagaa
     8401 cgcttggatg tattacttgt aaaaagaaat ctggcggagt caagggaaaa ggcaaaagcg
     8461 atcattatgt ccggaaatgt ttttgtggag ggacaaagag aagataaagc gggaagcacg
     8521 ttcccggaag aagtcagtat cgaagttcgg gggcatacac ttccgtatgt aagcagaggc
     8581 ggcttgaaat tagaaaaagc gatcgcaaat tttgatgtca gcgtaaaaga taaagtctgt
     8641 acggatgtcg gttcttcaac gggcggtttt acagattgca tgcttcaaaa cggtgcaaaa
     8701 aaggtgtttg cgatcgatgt gggacgagga cagcttgatt ggaaactgag aaacgacccg
     8761 agagttatct gcatggaaaa gacgaatatt cgatatgtga cacctgaaga tttgggagaa
     8821 gaaattgatt tttcttctat tgatgtctca tttatttcgc ttacgaaagt attggagccg
     8881 attcgtaatt atttggcaaa agacggtgag atcgtagctc ttattaaacc gcagtttgag
     8941 gcaggacggg aaaaagtagg aaaaaaaggt gtcgtaagag aaaagagcac acaccgggaa
     9001 gtgattcaga aaattatgga ttatgctatg tcgatcggtt tcgatctgtg tgctcttgat
     9061 ttttctccga tcaaaggacc ggaaggaaat atagagtatt tgattcattt aaaaaagagt
     9121 gaaaatgagg ccggtgaaaa tctcggcgga cttgatcctg ttattgtttc agacagagca
     9181 tttgagacgc tggcaaagca gcacgcttaa gtaatgtatg cgatgcagta ttttgtaaaa
     9241 acaagaggac taaaatggat tgcttttata ttattacaaa taaattaaaa gataaagatt
     9301 atgcgatcac gagtcagatt gaacaatata ttcaatcttc cggtaaaaaa tgttttttgt
     9361 cggaaaaaga cagtgagggc catattattc cgggaactgt tcctaaggaa gcagaatgcg
     9421 gattggtact aggtggtgat ggaactttaa tccgtgcggt cagagatctt ggagagcgaa
     9481 gccttccgct gcttgggatc aatcttggaa cgctcggata tcttacagat gtggatctgg
     9541 aagattttga aagtgcattg gatcatttgt tttcagagac tccggttatt gaagaacgga
     9601 tgatgctgga aggaagcttt cgtaattctc ggaaagatat ggcgatgaac gatattgtca
     9661 ttgcaagaga aggaaaggtt cgtatcgtaa gttttcatat ttatgtcaac ggggcgcttc
     9721 tgaatactta tcacgcagat ggagtgatca tatcgactcc aaccggaagt acaggttata
     9781 atctgtcggc gggaggcccg atcgttgaac cgacagcaca gatgatcgtg atcactccga
     9841 tttgttcaca cgcgcttaat acaagcagta ttgttctgtc agccgatgat ttgattgagg
     9901 tagaagtgtg cgaaggacgt tatggaaggc aggaacaggt ttctctttgc tttgacggtg
     9961 cagaacagac aactcttgtc acgggagaaa gagtttgtat taagcggtcg tctcatacgg
    10021 caaaactgat caaattaagc aaagaaagct ttatgaaaac aatgcgtaaa aaaatgaaag
    10081 gaaattgaac atatgaaagt cagccgtcat gcaaaaatta ttgaacttat cagtcagtat
    10141 gatattgaaa cacaggaaga actggcagaa tatctgaata atgcggggtt taaagtgacg
    10201 caggctactg tgtcgagaga tatacgtgat ctgaagttgt caaaagtatc tacaggaagc
    10261 ggacatcaga aatatattct tcacaggatc gaagaacccg gaatgagtga aaaatatata
    10321 cgtgtcttaa aagatgggta tgtggcgatg gatatggcgc agaatattct agtgatcaag
    10381 actgtttccg gtatggcaat ggctgtggct gccgctgtag acgcaatgaa atggcatgag
    10441 gtcgtaggct gtattgcagg agacgatacg atcatgtgtg caatcagaac tgtagaagat
    10501 acagcggccg taatggataa gataagaaag atcgtttcaa gagaagtata ggtgaatggt
    10561 atgctacaaa atctacatgt gaaaaatatt gctttgatcg atgagacaga agtagatttc
    10621 ggacaaggat tgaatatatt gacaggagaa accggggcgg gaaagtctat cttgctcggc
    10681 tctgtcggac tggctcttgg aggaaagtat agttctgatc ttttgagaaa cggggcggaa
    10741 aatggtctgg tagagctgac tttttcggtt gacgacggac agatcagaca aaaactggaa
    10801 gagatggata ttattccgga tgacggtatg gttactttaa caagaaagtt tacaggaagc
    10861 agaagcataa gccgcattaa tggagagaca gtgaatacag gaaagttgaa agaagcggca
    10921 gagcttttga ttgacattca tggtcagcat gataataaaa ccttacttca aaggaagaat
    10981 catttggtgc ttctggatct ttttgggaga aaagagattg ctccgatcaa atcagaaatg
    11041 tcaaaatgtt ataaagaata tcgggcgatc tgtaaaaagg aggaagaaac ttctttaaat
    11101 gatgaagaaa ggcgaagaga actttctctt tatgaatttg aagtacacga gatcgaagag
    11161 gctgctcttc gggaaaatga ggatgaagaa ttagaggaaa cataccgaag gatgacggag
    11221 agcagaaaaa tcacggatgc agttgcgcag acttatcgct atacatgcga ggacagttcg
    11281 gcaaatgcag gtgattgtct tactcgggcg atacgtgcgt ttcgggaggc ggcggaattt
    11341 gatgaagagg gagaccgtct gtgcgctctg cttatggatg tggacagcct tctgaatgat
    11401 tttaacagag aactgtccga atatgcctca aaatttgaat tttcagaaga agagttcaaa
    11461 gagacagagg aacggttgaa tctgatcaat catttaaaag caaaatatgg aagttctgta
    11521 agtgatgtcc ttgcatattg cgaatcaaaa cgccatagaa tagaacagct taatgattat
    11581 gatgcatata taaaacaaat tgaagaagag aaagaaaaag cgttaaaaaa agtagaacgt
    11641 gtaacgaaaa aacttcatga tgtaagaaat cgatataaaa cagtttttgc tgacgaaatc
    11701 tgtattcatc tgaaggaatt aaattttctc gatacaaggt ttgagataca tttaaaagat
    11761 gcaggacagt ttagcgcgaa cggaaaagac gaagcggaat tttatcttgc gcttaatccc
    11821 ggcgagcctg taaagccgct tgagaatatt gcatcaggcg gagaactttc ccgtattatg
    11881 cttgcgataa aaacggttct ggcagatgcg gaagatacgc cgactttgat t
//
LOCUS       DBMDXF010000043        11892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1541_length_11892_cov_1.977695, whole genome shotgun sequence.
ACCESSION   DBMDXF010000043 DBMDXF010000000
VERSION     DBMDXF010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_11892_cov_1.977695"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            282..1628
                     /locus_tag="ACZPD4_03030"
     CDS             282..1628
                     /locus_tag="ACZPD4_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1378082.1"
                     /translation="MKEKEKKNTIVKRMIFAVAAGFVVGFLMLFLREFLLESGKSGIW
                     KTVDAIFFQDVTKTNGIEGIGILYIVGQLFMRGLQLAIVPLVLTSLSLAMCSLANPER
                     LGKIAGKTFITYICFYVVAAALAGAAAYFVKTMGWFDVQLPNEMVTEAVTMEGYNPLV
                     TVVNAVPSNIFSAFSSNNEIISVVVVAIILGLCMTVLGKKTEPLRKVIENLNDVVQLF
                     LNFLIDKIGPVAIFCMITRTLAVYGIEYIKPTLVWIVTTIVVSLVLVFTIYPVGIFLT
                     TRLNPIPFVKKTLKIGLFAAATNSSAATLPLNTKTCTEELGCSKEISSFVLPTGMTIN
                     MNGTTAMHMIAITFIATAAGIDIKPSTLAIAAFLSICTAMGTPAIPVAGTTMVYVVMT
                     GLGMSSELCMIGYSLVLAMNYLPGMAVITLNVIGDAATNVIVSFKEKVLNKDVYYK"
     gene            complement(1824..4376)
                     /locus_tag="ACZPD4_03035"
     CDS             complement(1824..4376)
                     /locus_tag="ACZPD4_03035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1378083.1"
                     /translation="MNYGKNNLSKRKSSISSKKKMKKKRVGVRFFKAVITCMLLLVVI
                     GLVGGIIFAKKIIDNTPDVSAEDIQPKGFTTTIVDQNGTVIDTLKDSDSNRVYRTYDE
                     ITAKTDFLPHAFVAIEDERFYEHNGIDIQGILRAGVVGVMNGFDFTEGASTLTQQLIK
                     NNVFDFVNEKTFFDRVERKLQEQYLALAFEKEMNKNEILEAYLNTINLGQGCLGVQTA
                     STRYFNKDVADLTLSECAVIAAITQNPTEYDPVTNPESNAKRREKVLGNMLEQKYIDQ
                     AQYDEALADPVYDRILQTAAITEDTTPYSYFTDALIEDVIQDLMDEKGYSETQAYNLL
                     YSEGLTIKSTQDLAIQQICDEEASNDAYYPIVEYGITDYALTIHRADGSAENYSKENL
                     KQYLISVRGSAAPFVFSSEDEAKASIEQYKGTLGINTDAGDIVDERLNITLQPQVSVV
                     LMDQYTGHVKAIVGGRGAKTTSLSLNRATASPKQPGSCFKVLSTYAPGLNECGMTLAT
                     TIKDEPYRYASGQPVNNWDNKYIGSARVRYAIEHSMNVCAVRTLTETVGLEKGYKYLE
                     NFGFTTLVNNDPNYPGMSDIAQATALGGITRGVYNIEMTAAYAAIANSGIYTEPLLYT
                     EILDHDGNVLIDNSKPITRRVLKESTAFLLTSAMQDVINRGTGTPARLNNMPAAGKTG
                     TTENSTDLWLSAYTPYYTCSVWTGYDEDKTLEQFWNQSWHEVIWKHIMDRIHAELEYK
                     NFTPPASVVQRTICTQTGKLAVNGCPSITEYFDKDTLTKETCPGHGKTDDESKDDENN
                     EDNSTNNNSGSSENLGNTNGDDGDDVDPIPPTPDPEPNPEPEGPEVIPQSRN"
     gene            4597..5250
                     /locus_tag="ACZPD4_03040"
     CDS             4597..5250
                     /locus_tag="ACZPD4_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR1378084.1"
                     /translation="MECYHTVYTEGTGEIVEKKSRFIAEVFPVSNEEEAFAHLEEIRK
                     KHWDARHHCWAYVIGRNPAAERMSDDGEPAGTAGKPILEVLRGKKLTDVFVVVTRYFG
                     GTLLGTGGLVRAYTSAAAEALAHTVIITKIHGFKLKIITDYTGLGKIQYILGQRGLSV
                     LDTVYTDKVELTVLVPDDEEGFLMKELMEGTNGQAMTEKTEQCWFAETEEGIKIFEQ"
     gene            complement(5346..5975)
                     /locus_tag="ACZPD4_03045"
     CDS             complement(5346..5975)
                     /locus_tag="ACZPD4_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1378085.1"
                     /translation="MSDKIIENNQVTVIGSVASEFTYSHEVFGEGFYMVDILVKRLSN
                     SSDRIPVMVSERLIDVTQDYKGRSIMVSGQFRSYNRHEEQKNRLVLSVFAREMEFIEE
                     EPDGAKTNHILLEGYMCKTPVYRKTPLGREIADLLLAVNRPYGKSDYIPCICWGRNAR
                     YASNFDVGEHVRILGRIQSREYVKKLSETETETRTAYEVSVSKLECIDE"
     gene            6225..8051
                     /gene="typA"
                     /locus_tag="ACZPD4_03050"
     CDS             6225..8051
                     /gene="typA"
                     /locus_tag="ACZPD4_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR1378086.1"
                     /translation="MKTKREDVRNIAIIAHVDHGKTTLVDELLKQSGVFRENQEVAER
                     VMDSNDIERERGITILSKNTAVHYKGTKINIIDTPGHADFGGEVERVLKMVNGVILVV
                     DAFEGAMPQTKFVLRKALELSLPVIVCINKIDRPEARPDDVIDEVLELFIDLGASDDQ
                     LECPFVYASAKAGVACLDLSDPQQDMKPLFETILEYIPAPEGDPEAPTQVLISTIDYN
                     EYVGRIGIGKVDNGTISVNQDMILVNHHDPDKQQKVKISKLYEFDGLSKTEVKSATIG
                     SIVAISGISDISIGDTLCSPEKAEAIPFQKISEPTIAMQFIVNDSPFAGQEGKYVTSR
                     HLRDRLFKELNTDVSLRVEEMENTDSFKVSGRGELHLSVLIENMRREGYEFAVSKAEV
                     LYHTDENGKKLEPIETAYIDVPDEFTGVVIEKLGQRKGELRNMGVSNGGYTRLEFSIP
                     SRGLIGYRGEFMTDTKGNGIINTSFDGYEPYKGDIQYRKQGSLIAFETGESVTYGLYS
                     AQERGTLFIGPGEKVYSGMVIGQNGKTDDIELNVCKTKHLTNTRSSSADEALRLTPPK
                     ILSLEQALDFIDTDELLEVTPKTLRIRKKILDSRMRKRSMMK"
     gene            8205..8762
                     /gene="hpf"
                     /locus_tag="ACZPD4_03055"
     CDS             8205..8762
                     /gene="hpf"
                     /locus_tag="ACZPD4_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR1378087.1"
                     /translation="MNFIISGKNIEVTPGLKEAIEQKLGKLERYFTPETEIIVTLSVE
                     KGRQKIEVTIPVKGSIIRSEQTSSDMYVSIDLVEEVIERQLRKYKNKLVAKSQGHPTA
                     AVPSGSIKKEFIESDDESSDDDEIRIVRTKKFGIKPMFPEDACIQMELLGHSFFVFSN
                     AETDEVNVVYKRKDGSFGLIEPEFS"
     gene            8908..9417
                     /locus_tag="ACZPD4_03060"
     CDS             8908..9417
                     /locus_tag="ACZPD4_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378088.1"
                     /translation="MKDKIKLLSIAAVFVLLICAGYSILSFKQTDLQSILKKESSKKD
                     LFIDHNESRVCSIQYEAKDNRTEKIYFEFADKYIFDMESKFSVYYDSNYLMIDSFEYE
                     VLKNNLIHTKCIEKGNAPASSYHGVISWYNKFNVAGCNVTKVTIGFKVIDPEIDLSNI
                     TEILSLHEE"
     assembly_gap    9616..9625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9698..10456
                     /gene="dapB"
                     /locus_tag="ACZPD4_03065"
     CDS             9698..10456
                     /gene="dapB"
                     /locus_tag="ACZPD4_03065"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1378089.1"
                     /translation="MIKIMMHGCNGKMGRMITEIVKNDENAVITAGVDTYTETPNDYP
                     VFDSVEKCTEDADVVIDFSNAGAVDSLLDYCVDKKLPVVLCTTGLSEEQLAKAEKAAE
                     KTAVLKSANMSLGINLLLKLLKDAAKVLAPAGYDIELVEKHHNQKLDAPSGTALALAD
                     SVNEALGNEYHYVYDRSTVRQKRDAKEIGISALRGGTIVGEHEVIFAGTDEVIEFKHT
                     AYSRSVFGKGAVEAAKFLAGKESGMYDMSDVVGL"
     gene            10520..>11892
                     /locus_tag="ACZPD4_03070"
     CDS             10520..>11892
                     /locus_tag="ACZPD4_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HIR1378090.1"
                     /translation="MKEEYKELKELQKRYLTRLSDLYPTIAAASTEIINLQAILNLPK
                     GTEHFLTDVHGEYEAFSHVLKNGSGSVRRKIEDVFGNTMSAADKKSLATLIYYPRAKM
                     DLIRQTETNMEDWYKVHLYRLIEVAKRAASKYTRSKVRKALPPNFAYVIEELITEKAE
                     VHDKESYYNEIISTIIRIGRAEDFIEAISELIQRLVVDHLHIVGDIYDRGPGPHIIMD
                     KLMSYHSVDIQWGNHDILWMGAAAGQWGCIANVIRICARYGNLDILEDGYGINLLPLA
                     AFALRIYGDDPCICFRLKAVEGIDPDEMQMNMRIHKAISIIQFKVEGQIIRRQKAFHL
                     ENRALLHRIDFEKGTIELDGKKYPLLDTAFPTVDPKDPYAFTQEEEEIMKRLEKAFLH
                     CEKLQRHMRFLLNKGSLYKVYNDNLLYHGCVPLTEDGKLKEIRLFGKSYGGKELYDVL
                     DSYVRKG"
BASE COUNT         3554 a         2181 c         2785 g         3362 t
ORIGIN      
        1 tgtagcggtc attccttttt tatataggta ttaaattatg tgtagaaaac aatcataaac
       61 aaaatgtatg aatcaaagaa aatagaagaa aacaaaaaaa tatataacaa aaataacgga
      121 tgaaatctgt ttaaaacata gaaaatcttt gaatcaaaga aataatattg acaataattt
      181 gccgaaaatg tacaataaat atcaagaaat cccattgtat tcgacgatat agacataaag
      241 ggataaaaac gaaaaacaca gaaagttaag gagatggaga gatgaaagaa aaagaaaaaa
      301 agaatacgat cgtgaaacgt atgatattcg cggtggcagc cggattcgtg gttggttttc
      361 tcatgctctt tttgagggaa tttcttctgg aaagcgggaa atcaggaata tggaaaacag
      421 tggatgcaat cttttttcaa gatgtaacaa agacaaacgg aattgaaggt atcggtattt
      481 tatatattgt cggtcagttg tttatgagag gacttcagtt ggctattgtt cctcttgtac
      541 ttacttcgct gagtctggcg atgtgcagtc tggcaaatcc ggagagactc ggaaagattg
      601 cagggaagac atttatcaca tacatttgct tttatgtagt ggcagcggct cttgcgggag
      661 cagcggcata ttttgtgaaa acaatgggat ggttcgacgt acagcttcct aacgagatgg
      721 tgacagaagc ggtgacgatg gaaggatata acccgctggt gacagtcgtc aatgcagttc
      781 cgtctaatat tttttcggca ttttcatcta ataacgagat catatctgtc gtagttgttg
      841 cgatcattct cggattgtgc atgacagtgc ttggcaaaaa gacagaacct cttcgtaaag
      901 tcattgaaaa tttaaatgat gtcgtacaac ttttcttgaa tttcctgatc gataagatcg
      961 gaccggttgc aattttttgc atgatcacaa gaacgcttgc ggtgtatgga atcgaatata
     1021 ttaaaccgac gcttgtatgg atcgttacta cgattgttgt aagtctggtg cttgtattta
     1081 caatttatcc ggtagggata ttccttacaa cccggctgaa tccgattccg tttgtgaaga
     1141 aaacgttgaa gatcggcttg tttgcggcag cgacaaactc ttctgcggca acacttccgc
     1201 tgaatacaaa gacatgtacg gaagaactgg gatgttcaaa agaaatcagc agttttgttc
     1261 ttcctacagg tatgacgatc aacatgaacg gtacgacagc gatgcatatg attgcgatta
     1321 catttattgc gacagcggca ggaattgata ttaagccttc aacacttgcc atagcagctt
     1381 ttctttctat ctgcacggcg atgggaactc cggcaattcc ggttgcggga acgacgatgg
     1441 tgtatgttgt catgacaggg ttgggaatga gctctgaatt atgtatgatc ggatattcgc
     1501 ttgtactggc gatgaattat cttccgggaa tggcggtaat taccttgaat gtaatcggtg
     1561 acgcagctac aaatgtgatc gtaagcttta aagagaaagt gctgaataaa gatgtttatt
     1621 ataaatagaa gataaataaa attgtaaaag tacagaataa agaagttgtt tagaaaaaaa
     1681 gaagataatg aaatcttgta atatcatgat gaaacaaaaa acaggaatcg ccgcattgaa
     1741 cttagttcag tgcggcgatt cccgcttttg cgtgtaaaca tcccactatg agattcgcag
     1801 acagttaaga gatgttacgg caatcagttt cgagattgag gaatgacttc aggcccttcc
     1861 ggttcgggat tcggttcggg atccggcgta ggcggaatcg gatccacatc atcgccatca
     1921 tctccgtttg tatttcctaa gttttcggag ctgcctgaat tgttgtttgt cgaattatcc
     1981 tcgttgttct cgtcatcttt actttcgtca tctgtctttc cgtgtcccgg acaagtttct
     2041 tttgtcaaag tgtctttatc aaaatactct gtgatggaag gacatccgtt cactgcaagt
     2101 tttcctgtct gggtacagat tgttctttgt acaacagaag caggaggagt gaagttttta
     2161 tattctaatt ctgcgtggat gcgatccata atgtgcttcc atattacttc gtgccatgac
     2221 tgattccaaa attgttccag tgttttatcc tcgtcatatc cggtccatac agaacaggtg
     2281 tagtaaggcg tatatgcgga taaccagaga tcagtactgt tttccgttgt acctgtttta
     2341 cctgctgcag gcatgttgtt aagtcttgca ggcgttccgg ttccacggtt gattacgtcc
     2401 tgcatagcgc ttgtcagaag aaatgctgtc gattctttta aaacacgacg tgttataggt
     2461 tttgaattat cgatcaggac gttgccgtcg tgatcaagaa tttctgtata aaggagcggt
     2521 tcggtataaa ttccgctatt tgcgatcgcg gcataagcgg cggtcatttc aatattgtaa
     2581 acacctcttg taatacctcc gagggcggta gcctgtgcga tatccgacat acccggatag
     2641 ttcggatcat tatttacaag cgtcgtaaaa ccgaagtttt cgagatattt gtatcccttt
     2701 tcaagtccga ctgtctcggt cagagtgcgt acggcgcaaa cattcatgga gtgttcgata
     2761 gcatagcgta cgcgggcgct tccgatgtat ttgttatccc agttgtttac cggctgtccg
     2821 ctggcgtacc ggtacggctc gtctttgatc gttgttgcaa gagtcatgcc gcactcgttt
     2881 aatcctgggg cataagtgga taaaacttta aaacaagatc ccggctgttt tggggaagct
     2941 gtggcgcggt ttaaagataa gctggtcgtc tttgctccgc gtccgccgac aatggctttt
     3001 acatgtcctg tatattgatc cattaatacg acggaaacct gaggctgaag cgtaatgttt
     3061 aaacgttcgt ctacaatatc gcctgcatct gtattaatac cgagagtgcc tttatattgt
     3121 tcgatagaag ctttcgcttc atcttccgaa ctgaaaacaa acggagcggc agagcctcgt
     3181 acagagatca gatactgctt taagttttcc ttactgtagt tttcggcaga tccgtccgca
     3241 cggtggattg tcagtgcgta gtctgtgata ccgtattcta cgatcggata ataagcatca
     3301 ttggaggctt cttcgtcaca gatctgttgg atcgccagat cctgagtaga cttgatagtc
     3361 aatccttcgc tgtagagaag gttataagcc tgtgtttccg agtaaccttt ctcgtccata
     3421 agatcttgaa tgacatcttc gatcaaggca tctgtaaaat aagagtaagg cgttgtatct
     3481 tcggtgatcg cggctgtctg taaaatgcgg tcatacacag ggtcggcaag cgcttcgtcg
     3541 tactgcgcct gatcgatgta tttttgttca agcatgttgc cgagaacttt ttcacggcgt
     3601 ttggcgttag attccggatt cgttacagga tcgtactctg tcgggttctg tgtgatcgcg
     3661 gcaatgaccg cacattccga aagggtcaga tccgccacat ctttgttgaa atatcttgta
     3721 gatgccgtct gtactccgag acatccctga ccgaggttga tcgtgttaag ataggcttct
     3781 aatatctcgt ttttgttcat ctctttttca aatgcgagag cgagatactg ctcttgtaat
     3841 ttacgttcca ctctgtcaaa aaatgttttt tcgttgacaa aatcaaatac gttgtttttg
     3901 ataagctgct gtgtcagggt actggctcct tcggtaaaat caaaaccgtt catgactccg
     3961 acgacgccgg ctcttaaaat tccctgaata tcgattccgt tatgttcata gaaacgttcg
     4021 tcctcgatcg ccacaaatgc atgcggaaga aaatctgttt ttgccgtgat ctcgtcataa
     4081 gtacggtaga cacggtttga atcagaatct ttcagagtgt cgatgactgt gccgttttga
     4141 tcgacaatcg ttgttgtaaa tcctttcggc tgaatgtctt cggcagatac gtccggggtg
     4201 ttgtcaatga ttttttttgc aaaaattata ccgccgacaa gccctataac gacaagcaga
     4261 agcatacagg tgataactgc tttaaaaaaa cgaactccta cgcgtttctt tttcattttc
     4321 tttttcgaag agattgatga ttttctcttt gaaagattat tttttccgta gttcacgaat
     4381 aacatcctcc ttttcaactt ctatgattat atcaaataag gtcttttaaa taaagaaaaa
     4441 aatgaaatct tcatatttta taaagaattt taatcataat atcagtatct tcacgttcgt
     4501 acaggcgaga gatccgcata atggttgtac atgttccatg tctttgctat agtagaaaag
     4561 gtatggaact gttttatgaa aaaggagaag ataaaaatgg agtgttatca tacggtatat
     4621 acagaaggaa caggagaaat tgtggaaaaa aaatcccgtt ttatagcaga ggtatttccg
     4681 gtttcaaatg aagaagaggc gtttgcgcat ttggaagaaa tccgaaaaaa acactgggat
     4741 gccagacatc actgctgggc atatgtaatc ggaagaaatc cggcggcaga gagaatgagc
     4801 gatgacggag aaccggcagg aacggcgggg aagccgatac tggaagtgtt aagagggaaa
     4861 aaactgacag atgtatttgt tgttgtcacc agatactttg gcggcacgct tctcggaaca
     4921 ggcggtcttg taagagcgta tacgtcggcg gcggcagagg cgcttgctca tacggttatt
     4981 ataaccaaga tacatgggtt taaactgaaa attattacag actatacggg acttggaaaa
     5041 attcagtata ttttaggaca aaggggatta tccgttcttg atacagtgta tacagataaa
     5101 gtagaactta cagttcttgt gccggatgac gaagagggat ttctgatgaa agagctgatg
     5161 gaaggcacga acggacaggc gatgacggaa aaaacggagc agtgctggtt tgcggagaca
     5221 gaagaaggaa taaaaatttt tgaacaataa aaagaacgct gcttacaggc gacagtctgc
     5281 ctgtaagcag cgttccttat ttgtataggc aacgagcaaa agcccggatt tgctaaatga
     5341 tgagattact catcaataca ttccagtttg ctcactgaca cttcatatgc cgttcttgtc
     5401 tctgtttctg tttcggataa tttctttaca tattctctgc tttggatcct tcccagtata
     5461 cgcacatgtt ctcctacatc gaagttcgat gcgtaccgtg cgtttcttcc ccagcagata
     5521 caagggatat aatctgattt cccgtaaggg cggtttacgg caagaagcag atccgcaatc
     5581 tcacgtccta aaggggtttt tcgatatacc ggtgtcttgc acatatatcc ttccagtaaa
     5641 atatgatttg tctttgcgcc gtccggttct tcttcgatga actccatttc ccgtgcaaac
     5701 actgaaagaa caagtctgtt tttctgttct tcatgacggt tatatgaccg gaattgtcct
     5761 gacaccataa tgcttctgcc tttgtaatct tgcgttacat cgatgagtcg ttccgagacc
     5821 atgaccggga tcctgtcact ggaattgctc agccgtttca cgagaatatc taccatataa
     5881 aatccttctc cgaatacttc atggctgtat gtaaattccg aagccaccga tcctataacc
     5941 gttacctgat tgttctcaat aatcttatct gacatgctct gtaattctcc tctcctctta
     6001 tcgccggggt atctgtccgg cactgcttgt atttttgagt cactactaaa tttatgtttt
     6061 tttcatcttg tttagaactg atttcataca gaatcgttcg acacaaaatt tcgacatgcg
     6121 ttcttgtaaa atgaaaattt tatgataaaa tataacagtt gcagtaaaac tataactata
     6181 aatcactccc ttagcagggg aaaataaaga aagagtgtat atttatgaag acaaaacgtg
     6241 aagatgtaag aaacattgcc atcatcgccc atgttgacca cggaaagacg acattggttg
     6301 acgagctttt aaaacaaagc ggtgttttcc gtgaaaatca ggaagtggcg gaacgtgtta
     6361 tggactcgaa cgatattgag cgggaacgtg ggatcacgat cctgtccaaa aatacggcag
     6421 ttcattataa aggaacaaag atcaacatta tcgatacgcc gggacatgcg gattttggcg
     6481 gagaagtaga acgtgtgctg aaaatggtca acggcgtgat cctcgttgtc gacgcatttg
     6541 aaggcgcaat gccgcagaca aaatttgtat taagaaaagc gcttgaatta agccttcctg
     6601 tcattgtctg catcaacaag atcgaccgtc ccgaagcaag acctgacgat gtaatcgacg
     6661 aagttcttga acttttcatc gatcttggcg catctgacga tcagcttgag tgcccgtttg
     6721 tatatgcatc cgccaaagca ggagttgcat gccttgatct gtctgatccg cagcaggaca
     6781 tgaaaccgtt atttgaaacg atcctcgaat atattcccgc tccggaagga gatcctgaag
     6841 ctccgacgca ggtgctgatc agcacgatcg attacaacga atatgtcgga cgtatcggta
     6901 tcggaaaagt ggataacgga acgatcagtg tgaatcagga tatgatactt gtcaatcacc
     6961 atgatccgga caaacagcag aaagtaaaga tcagtaaatt atatgaattt gacggattga
     7021 gcaaaacaga agttaaaagt gcaacgatcg gatcgatcgt cgctatttcc ggtatttctg
     7081 atatatcgat cggagatacg ctttgttctc ctgaaaaagc agaggcaatt cctttccaga
     7141 aaatttcaga accgacgatt gcgatgcagt ttattgtaaa cgacagtcct tttgccggac
     7201 aggaaggaaa atatgtaact tcccgccatc tgcgtgaccg cctgtttaaa gagctgaaca
     7261 cagacgtaag tcttcgagtg gaagaaatgg aaaatacaga cagttttaaa gtgtcgggac
     7321 gcggagaact tcatttgtcc gttttgattg aaaatatgcg ccgcgaagga tatgagtttg
     7381 cagtaagcaa agcggaagtt ctctatcata cggatgaaaa cggaaagaaa cttgagccga
     7441 tcgagacagc ttatattgat gtgccggatg aatttacagg agttgtaatc gaaaaactcg
     7501 gccaaagaaa aggagaactt cgcaatatgg gggtttccaa cggcggttac acacgtcttg
     7561 agttttccat tccgtccaga ggattgatcg gatatcgcgg agaatttatg acagatacaa
     7621 aaggaaacgg tatcattaat acaagcttcg acggatatga gccgtacaaa ggcgatatcc
     7681 agtatcgtaa gcagggatcg cttatcgcat ttgagacagg tgaatctgta acttatggtt
     7741 tatacagcgc gcaggaacgc ggaactttgt ttatcggacc gggagaaaag gtttattcag
     7801 gtatggtgat cggccagaat ggaaaaacag atgatattga gttgaacgta tgtaagacaa
     7861 agcatctgac aaatacacgt tcctccagcg cagatgaagc tttgagactg actccgccta
     7921 aaattttaag tcttgagcag gctcttgatt ttatcgacac cgacgagctt ttggaggtga
     7981 cgccgaagac gctgcgtatc cgcaaaaaaa tcctggattc aaggatgcgg aagagaagta
     8041 tgatgaaata aattatgaaa atttaatgaa atttgaagaa tttacagaat tcaaaacgat
     8101 ttattatata cattagagct gttctgtgtt ataatatttt cataatataa acatcctttt
     8161 tacacatgtt tatgttaagt atatacaaaa aggagttgaa cagcatgaat tttatcatca
     8221 gcggaaaaaa cattgaagta acaccggggc ttaaagaggc tatcgaacaa aagttaggaa
     8281 aacttgagag gtatttcact cctgaaacag aaatcatcgt aacgcttagc gtggaaaaag
     8341 gacgtcagaa aatcgaagtg acgattcctg taaaaggatc tatcatccgc tccgagcaga
     8401 cgagcagcga catgtatgtg tcgatcgacc ttgtagaaga ggttattgaa cggcagcttc
     8461 gtaaatataa aaacaaactt gttgcgaaaa gccagggaca tcctactgct gctgttccgt
     8521 caggaagcat taaaaaagag tttatcgaat ccgacgacga gtcatctgat gacgatgaaa
     8581 tccgcatcgt ccgcacgaaa aagtttggta ttaaaccgat gttccctgaa gatgcctgca
     8641 tccagatgga actgctcgga catagctttt tcgttttctc aaacgcagaa acagatgaag
     8701 taaatgttgt atacaaaaga aaggacggat ctttcggact gatcgagccg gaattttcat
     8761 aaactgaccg aattgtcatt tttcatcagt aaacagaatt aacgtacaga acgcccgcaa
     8821 aactgttcgc actgttttgc gggcgctttg actgttgtgt agctgccccc cgataagttg
     8881 tcttgaaagg tttatgagga acttcacatg aaagataaaa tcaaattatt gtcaatagcc
     8941 gcagtatttg ttttattgat atgcgcggga tattcgatac ttagttttaa gcagactgat
     9001 ttacaaagta tcttaaaaaa ggaaagttct aaaaaagatt tatttattga tcataatgag
     9061 agcagagttt gttccataca atatgaggcg aaggataacc ggacagagaa gatttatttt
     9121 gaatttgccg ataaatatat atttgatatg gagtctaagt tttcggttta ttatgacagc
     9181 aattatttaa tgatagactc ttttgaatat gaggtgttaa aaaacaatct gatccatacg
     9241 aagtgtattg aaaaggggaa tgcacctgca tcgtcatatc atggagttat atcatggtat
     9301 aataaattca atgtagccgg ctgtaacgtg acaaaagtta cgataggttt caaggtgatc
     9361 gatccggaaa tcgacctgtc aaatattaca gagattttat ctttgcatga ggaataaaga
     9421 cgggtatgat ctgacgaacc ggttatcgag aaactgtaac agttcagccc acgccattcg
     9481 taaaaccgca ctgtgcgctt atcgtggctg aattgttacg agaaactttg tgaaaaggat
     9541 atcatatgtt gagatgcgaa ctcaaatgtg atatcctttt ttgagtagag aaaaatccgt
     9601 ttttgattca ataatnnnnn nnnnngccgc aggaaaaaaa cttgtagtga acgattgttt
     9661 ttgaaacgtg aaatgataca ggcaggagga aaataaaatg attaaaataa tgatgcatgg
     9721 atgcaacgga aaaatgggaa ggatgatcac ggagatcgta aaaaatgatg aaaatgccgt
     9781 tattacggca ggtgttgaca catatacaga aacgccgaat gattatccgg tatttgattc
     9841 ggtggaaaaa tgtacggaag acgcagatgt tgtgatcgat ttttcaaatg cgggagcagt
     9901 ggattcgctt ttggattatt gcgtggataa gaaacttccg gttgtgcttt gcacgacagg
     9961 actttcggaa gagcagcttg caaaagcaga gaaggcggca gagaagacgg cggtgttaaa
    10021 gtcggcgaat atgtctcttg gaattaatct ccttttaaaa cttttaaaag atgcggcaaa
    10081 agtgcttgct ccggcgggat atgatattga acttgtggaa aaacatcaca atcagaaact
    10141 ggatgccccg agcggaacag cgcttgcact tgcggattcg gtgaatgaag cgcttggaaa
    10201 cgaatatcat tatgtatatg acagaagcac tgtccgccag aaacgtgatg caaaagaaat
    10261 cggaatttca gctcttcgcg gaggtacgat cgtaggggaa catgaggtga tctttgcggg
    10321 aacagacgaa gtgatcgaat ttaaacatac ggcatactcg agaagcgtat tcggaaaagg
    10381 cgctgtcgag gcggcaaaat tccttgccgg aaaagaaagc ggtatgtatg atatgagcga
    10441 tgtggtcgga ttgtgacatc cgacgtacat gagagatata aattgtgcgg tgtattgtgg
    10501 aacaaggggg cagacaagca tgaaagaaga atataaagaa ctgaaagaac tccagaaacg
    10561 gtatttgacc cgattgtcag acttatatcc gacaatcgcg gcggcatcga cggagatcat
    10621 caatcttcag gcgattttga atctgccgaa aggaacagaa cattttctga cagatgtaca
    10681 cggtgagtat gaggcttttt ctcatgtttt aaaaaacgga tccggatctg tccgaagaaa
    10741 gatcgaggat gtgttcggaa atacgatgag tgccgcagat aagaaatctt tggcaacgct
    10801 gatctattat ccgagagcaa agatggacct gatccggcag acagagacga atatggaaga
    10861 ctggtataaa gtacatctat atcgtttgat cgaagttgcc aaaagagcgg cgagcaagta
    10921 tacgcggtcg aaagtaagaa aggcgcttcc cccaaatttt gcttatgtga tcgaggaact
    10981 gatcacggag aaagcggagg tacatgataa ggaatcatat tataatgaga ttatttcaac
    11041 gattatacgg atcggaagag cagaagattt tatcgaggca atttcagagt tgatccaaag
    11101 gctcgttgta gatcatctgc atattgtcgg cgatatttat gacagaggtc ccggtcctca
    11161 tattattatg gataaactga tgtcttatca ctctgtcgat attcagtggg gaaatcatga
    11221 tatactttgg atgggagcgg ctgcgggaca atggggctgt attgcgaatg tgatccgcat
    11281 ctgtgcacgt tacggaaatc tcgatatttt ggaggatgga tatgggatca atcttcttcc
    11341 tctggcagca tttgcccttc ggatttacgg ggatgatccg tgtatttgtt ttcggttaaa
    11401 agcggttgaa gggatagatc ctgatgagat gcaaatgaat atgaggattc ataaagcaat
    11461 ctccatcatt caatttaaag tggaagggca gatcatccgt cggcagaaag catttcattt
    11521 agaaaacagg gcgcttttgc acagaattga ttttgaaaaa ggtacgattg aactggacgg
    11581 aaagaaatat ccgcttttgg atacggcgtt tcctacagtc gatccgaaag atccgtacgc
    11641 ttttactcaa gaggaagagg agatcatgaa gcggcttgaa aaggcgtttc tgcactgtga
    11701 gaagcttcaa aggcatatga ggtttttgct gaacaaggga agtttgtata aagtttacaa
    11761 tgataatctt ctctatcacg gatgtgttcc gctcacagaa gacggaaaat tgaaagaaat
    11821 tcggcttttc ggcaagtctt acggtggaaa agaactttat gatgttctgg attcttatgt
    11881 gagaaaaggc tt
//
LOCUS       DBMDXF010000044        11774 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1556_length_11774_cov_2.096719, whole genome shotgun sequence.
ACCESSION   DBMDXF010000044 DBMDXF010000000
VERSION     DBMDXF010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 11774)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11774)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11774
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_11774_cov_2.096719"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="ACZPD4_03075"
     CDS             complement(<1..527)
                     /locus_tag="ACZPD4_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1378091.1"
                     /translation="MSRHPELLIPASSLEVLKTAVIFGADAVYIGGEAFGLRAKAKNF
                     STEEMRKGIQFAHEHGVKVYVTANILAHNDDLAGVREYFEELKEIKPDALIIADPGVF
                     EIAKEICPEIERHISTQANNTNYATYNFWYKQGASRVVSARELSMEELKELRANIPED
                     LEIETFIHGAMCISYS"
     gene            complement(590..1255)
                     /locus_tag="ACZPD4_03080"
     CDS             complement(590..1255)
                     /locus_tag="ACZPD4_03080"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR1378092.1"
                     /translation="MIVDERIITFINSLDTKNSEILEDIEREALADNVPIIRREMQSF
                     LKVLLMVKKPMRVLEVGTAVGFSALLMSEYVPEECAITTIENYEKRIPIARQNFVRAG
                     KEKKITLLEGDAMDVLKTLEGPYDFIFMDAAKGQYIHYLPEILRLLAKGGILVSDNVM
                     QDGDVIESRFAVERRDRTIHARMREYLYELKHNDALVTSIIPLGDGVALSVKKEVGTD
                     IAQ"
     gene            complement(1259..>1480)
                     /locus_tag="ACZPD4_03085"
     CDS             complement(1259..>1480)
                     /locus_tag="ACZPD4_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1378093.1"
                     /translation="DIVSRGFVYVRESEELMDDARRAVSEAVDKCLSGRHTDWNKIKL
                     VIRDSMNDYIWKKTKRKPMVIPIIMDVDV"
     assembly_gap    1481..1490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1491..2940)
                     /locus_tag="ACZPD4_03090"
     CDS             complement(<1491..2940)
                     /locus_tag="ACZPD4_03090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1378094.1"
                     /translation="MKKQNNGKLRIIPLGGLEQIGMNITAFEYEDSIVVVDCGLAFPE
                     DDMLGIDLVIPDVTYLKDNASKVKGFVITHGHEDHIGALPYVLKEINLPIYTTKLTMG
                     IIENKLKEHNLLRTTKRKVVRHGQSINLGKFRIEFIKTNHSIQDASALAIYSPAGVVI
                     HTGDFKVDYTPVFGDAIDLQRFAEIGKKGVLALMSDSTNAERRGFTQSEKTVGITFDH
                     IFAEHQNTRLIIATFASNVDRVQQIINSAYKYGRKVVVEGRSMVNIIATASELGYLDV
                     PENTLIEIDQMKNYPPEKMVLITTGSQGESMAALSRMAADVHRKVTIQPNDTIIFSSN
                     PIPGNEKSVSRVINELSSKGAEVIFQDAHVSGHACQEELKLIYSLVKPKYAIPVHGEY
                     RHLKANAEVAKSLGIPKENVFIIQSGDVLELCDDSAEVVDKVHTGAILVDGLGVGDVG
                     NIVLRDRQHLAEDGIIIVVLTLERRTNRLLAGP"
     gene            complement(2957..3418)
                     /locus_tag="ACZPD4_03095"
     CDS             complement(2957..3418)
                     /locus_tag="ACZPD4_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1378095.1"
                     /translation="MKKNRTEELLKEKLREKGLKVTNQRILILSVLEQNKGRHLTAED
                     IYELVTQEHPEIGLATVYRTIQLLWDMQLIDKINLDEGCVRYEIGHLLDGGSKHNHHH
                     LICRTCNKIIPFDDDLLEEAERHIERTIGFHVLDHEVKFYGICRECMKKGK"
     gene            complement(3566..3826)
                     /locus_tag="ACZPD4_03100"
     CDS             complement(3566..3826)
                     /locus_tag="ACZPD4_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1378096.1"
                     /translation="MEKVKFAFADGNGEDEFFILEETKINGSAYILVAESEDDDAECL
                     ILKDVRDEESGESVYEIVENEKELQAVSKVFEELLEDVSIEL"
     gene            complement(3984..4421)
                     /gene="ruvX"
                     /locus_tag="ACZPD4_03105"
     CDS             complement(3984..4421)
                     /gene="ruvX"
                     /locus_tag="ACZPD4_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1378097.1"
                     /translation="MRIMGLDYGSKTVGVAISDALRLTAQAVETIERKEENKLRRTCA
                     RIEELIKKYDVEKIVLGFPKHMNNDIGERAQQSMEFGEMLKRRTGLEVILWDERLTTV
                     EAERTLIESNVRRENRKKHIDQIAAVFILQGYLDLMHMKKQAE"
     gene            complement(4428..4691)
                     /locus_tag="ACZPD4_03110"
     CDS             complement(4428..4691)
                     /locus_tag="ACZPD4_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR1378098.1"
                     /translation="MSDLSNTQFFQVEPGPQISAGDILEIVFKALKEKGYNPVNQIVG
                     YIMSGDPTYVTSFNGARSLIMKVERDELVEELLKAYIEHHSWE"
     gene            complement(4806..6101)
                     /gene="mtaB"
                     /locus_tag="ACZPD4_03115"
     CDS             complement(4806..6101)
                     /gene="mtaB"
                     /locus_tag="ACZPD4_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR1378099.1"
                     /translation="MKKAALHNLGCKVNAYETEAMQEMLEQAGYEIVPFKEGADVYII
                     NTCTVTNIADRKSRQMLHRARKLNPDAVVVAAGCYVQAQEGKEIDPCIDIVIGNNHKK
                     DLPELLKQYEMEKSGHTEYAMEDINRTKEYEELHLTKPGDHTRAYIKVQDGCNQFCTY
                     CIIPYARGRVRSRQTKDVLEEVRDLAGNGYKEVVLTGIHLSSYGIDFDGQRHLLDLIK
                     EVHKVEGIERIRLGSLEPGIITEEFAKELSEMPKVCPHFHLSLQSGCDATLKRMNRKY
                     TSGEYYDRCQILRKYFDHPALTTDVIVGFPGETEEEFEASYAFVDRVDFYETHIFKYS
                     KREGTKAAVMDGQIDEKIKGERSARMIALGERKKKAYEDSFIGKEVEVLFEERAQIEG
                     KAVMTGHTKEYMKIALETEKNISNCIVKVRIENHSQIIR"
     gene            6221..6454
                     /locus_tag="ACZPD4_03120"
     CDS             6221..6454
                     /locus_tag="ACZPD4_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1378100.1"
                     /translation="MKTVQISLNSIDKVKSFVNEITKYDNDFDLVSGRYVIDAKSIMG
                     IFSLDLSKPIDLNIHADGDVDAILAALDPYIIK"
     gene            complement(6613..7797)
                     /gene="thiI"
                     /locus_tag="ACZPD4_03125"
     CDS             complement(6613..7797)
                     /gene="thiI"
                     /locus_tag="ACZPD4_03125"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1378101.1"
                     /translation="MEFHSFLIKYGEIGIKGKNRYIFEDALVRQIRYALQSVDGEFLV
                     HKCHGRVYVDCEGEYDYEETVESLKKVFGIVGICPVVRKEVKDPEGLKKDVTAYVGEM
                     YPQADKTFKVEARRANKRYPMNSMELNCALGEAVLDAYPEMKVDVHDPEVRLNVEIRE
                     EVYIYSEIIPGPGGMPVGTNGSAMLLLSGGIDSPVAGYMVAKRGVELEAVYFHAPPYT
                     SERAKEKVIDLARLVSAYSGPIKLHIVNFTDIQLAIYEKCPHDELTIIMRRYMMRIAE
                     HFAKKNGCLGLVTGESIGQVASQTMQSLAATNAVCTLPVYRPLIGFDKKEIVEISEKI
                     DTYEVSIQPFEDCCTIFVAKHPVTKPNVERIEKSEINLADCIDDLMKTALETAEVITV
                     GA"
     gene            complement(7840..8997)
                     /locus_tag="ACZPD4_03130"
     CDS             complement(7840..8997)
                     /locus_tag="ACZPD4_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1378102.1"
                     /translation="MEVYLDNSATTMCYPEVGELVYHIMCRDYGNPSAMYRKGMEAER
                     YIKEAKETISKLLKVNAKEIIFTSGGTESDNQALIGCARANRRNGNHLITSCVEHPAI
                     LNTMHYLEEEEGFRVTYLPVDECGRIRLDALKEALCPETILVSVMYVNNEIGTVQPVA
                     EAARIVKQYKQSILFHTDAVQGFGKYKIYPKRLGVDMLSASGHKIHGPKGAGFLYVDS
                     RVKIKPIIFGGGQQKDMRSGTENVPGIAGLALASKMIYQDLELKTEHMRELKTHFIDC
                     ISQIGNVKVHGLTDEGSAPHIVSAGFAGVRSEVLLHTLEDRGICVSAGSACASNHPAI
                     SGVLQAIGTPREYLDSTLRFSLSEFTTKEEIDYTLETLYNCIPMLRKYTRR"
     gene            complement(9032..9799)
                     /locus_tag="ACZPD4_03135"
     CDS             complement(9032..9799)
                     /locus_tag="ACZPD4_03135"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR1378103.1"
                     /translation="MQQFFAEPSWITESEILIRGADLNHMKNVLRMKVGEDVRINDGQ
                     GTTYLCCLNRYEKDQAVLDVFRKLDTETELPSEIILFQGLPKGDKMEWIVQKSVELGA
                     HKIVPFSAKRSVVKLDEKKAVKKQERWQLIAKGAAEQSGRGVIPEVCGVCSFTEALEQ
                     AESLDVVLIPYELEEGMAETVRVIEQIEPGQSVGIFIGPEGGFEEAEVLRAKEKGARP
                     VTLGKRILRTETAGLTTLSILMYHIESRAVSPKREES"
     gene            complement(9819..10772)
                     /gene="prmA"
                     /locus_tag="ACZPD4_03140"
     CDS             complement(9819..10772)
                     /gene="prmA"
                     /locus_tag="ACZPD4_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1378104.1"
                     /translation="MKWNKFRLKTTTEAEDIVSSMLMDLGIQGVEIEDKIPLTQSDKE
                     QMFVDILPQTEADDGVAYLSFYLEPEEDKEKILSDVRKELEEMSAYVNVGECLIEESE
                     TEDVDWVNNWKQYFHQFYVDDVLIIPSWEEVKPEDEDKMIIHIDPGTAFGTGMHETTQ
                     LCIRQIRKYVTENTKILDVGCGSGILGMLALKFGAKYSVGTDLDPCAIDATHENMEVN
                     GISKDRYEVMIGNIIDDKEVQDKVGYECYDIVAANILADVLVALTPVVVNQIKKGGIY
                     ITSGIIDDKEETVVEAVKKAGLEVLDITYQGEWVCVTARKN"
     gene            complement(10776..>11774)
                     /locus_tag="ACZPD4_03145"
     CDS             complement(10776..>11774)
                     /locus_tag="ACZPD4_03145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1378105.1"
                     /translation="TDENGERTSLKIKISMRKTAKMIAVVSCTILMLAACSPKKEVIS
                     QSVTGNEDKTYDSGEWIVQKNGSLIQNAGGNQDGEKKRYCFQDVEENRYEAPLLSDVP
                     KCTYDFSDLKTDEQTGYKSYQDPETGAKAKLGIDVSEFQGEVIDWKQVKESGVEFVMV
                     RLGYRAYGESGSLVLDAMYEQNVKNALEAGLQVGVYFFSQAVSPAEAVEEAEFVLEHL
                     KHYNITGPVVFDTEEIKWDSARTDGNTRQDFTNYCKVFCDTIEHAGYDPMIYANLKWM
                     TFTLDMEQLTEYDFWYADYHETPQCPYEYKIWQYSETGAVPGITGNVDLNLWFQED"
BASE COUNT         2899 a         3241 c         1963 g         3661 t
ORIGIN      
        1 gaataagaga tacacatcgc cccgtgaata aacgtctcga tttccaaatc ttccggaata
       61 tttgcccgaa gttcttttag ctcttccatc gacagctctc tggcagatac gactcgactc
      121 gccccctgtt tataccagaa attataggtt gcataatttg tattatttgc ctgagtgctt
      181 atatgccttt ctatctcggg gcatatctct ttggcaatct caaatactcc cggatctgca
      241 atgatcagtg catccggttt gatttccttt aactcttcaa aatactcacg caccccggca
      301 aggtcatcgt tgtgcgccag aatattagcg gtcacatata ctttcacacc atgctcatgg
      361 gcaaattgaa ttccttttcg catctcttcc gttgaaaaat tcttcgcttt tgctctaaga
      421 ccgaaagctt ctcctccgat atagaccgca tcagctccga aaataactgc cgttttcaac
      481 acttccagac tgctcgccgg aattaataac tcaggatgtc tgctcatctt tctcttcatt
      541 ctcctacttc ttctttttct gtttttatgg cggcttataa ttttgcgctt tactgcgcaa
      601 tgtccgttcc gacttccttt ttcacactca gcgccacccc gtctcctaaa ggaatgatcg
      661 atgttacaag cgcatcgtta tgttttaatt cgtacagata ttcccgcatc cgcgcatgaa
      721 tcgtacgatc ccgccgttct accgcgaaac gtgactcgat cacatcaccg tcctgcatca
      781 cattatccga gacaaggata ccgccctttg caagcagacg cagtatctcc ggcagataat
      841 gaatgtactg acctttcgcc gcatccataa aaataaaatc atacggtcct tccaatgttt
      901 tcagcacatc catcgcatcg ccttccagca aagtgatttt cttctctttt cctgcacgga
      961 caaaattttg tcttgcgatc ggtattctct tctcataatt ttcaatcgtt gtgatcgcac
     1021 attcttccgg tacatattca ctcatcaata acgcagagaa acctacggca gttccgactt
     1081 ccagaacgcg cataggtttt tttaccataa ggagcacctt taagaagctc tgcatctcac
     1141 ggcgtattat aggcacgtta tcagccagag cctctctctc gatgtcctct aatatttcac
     1201 tgtttttcgt atccagcgaa ttgatgaatg taataatacg ttcatctact atcataattt
     1261 acacatccac atccatgatg atcggaatca ccatcggttt tctctttgtc tttttccaaa
     1321 tatagtcgtt catggaatcg cggatcacaa gtttgatctt attccagtct gtatgacgtc
     1381 ccgagagaca cttatccacc gcctcggata ccgctcttct cgcatcatcc atcagttctt
     1441 ctgattctct tacataaacg aagcctcgcg atacgatatc nnnnnnnnnn ccggacctgc
     1501 aagcaggcgg ttcgttcttc tctcaagcgt aagcacaaca atgataatac cgtcctctgc
     1561 caaatgctgt ctgtcccgaa gcacgatgtt tcctacgtct cctaccccga gtccgtctac
     1621 aagaatcgct cccgtgtgta ctttatctac tacttccgca ctgtcatcgc acagttccag
     1681 cacatctccg gactgaatga taaatacatt ttccttcgga attccaagtg attttgccac
     1741 ttccgcattt gctttcaaat gacggtattc tccgtggacc ggaatggcat atttcggctt
     1801 cacaagagaa tagatcaatt tcagttcttc ctgacaggca tgtcccgata catgggcatc
     1861 ctggaaaata acttccgccc ctttggaaga aagttcgttg atcacacggg aaacagactt
     1921 ctcatttccc ggaatcggat tagaactgaa gataatcgta tcgtttggct gtatcgtcac
     1981 ttttctgtgc acgtccgccg ccattctcga cagagccgcc atagactctc cctgacttcc
     2041 ggttgtgata agaaccattt tctccggcgg ataattcttc atctgatcga tctcgatcaa
     2101 tgtgttttcc ggcacatcca aatatcccaa ttctgacgct gtcgcaatga tattcaccat
     2161 gctgcgccct tccacaacta cttttcttcc atacttatat gccgaattga taatctgctg
     2221 cacacgatcc acgttagaag caaacgtagc gatgatcagc cttgtattct gatgttccgc
     2281 aaaaatatga tcaaacgtaa taccgaccgt cttttccgac tgggtaaatc ctcgccgctc
     2341 cgcattcgta ctgtctgaca tcagagcaag cacacccttt tttccgatct ccgcaaaccg
     2401 ctgtagatcg atcgcatctc cgaacaccgg cgtataatcg actttaaaat ctcctgtatg
     2461 gattacgaca ccggcaggcg aatagatcgc aagggcagac gcatcctgaa tactgtggtt
     2521 cgttttaata aattcaatcc tgaattttcc aagattgatc gactgtccgt ggcggacaac
     2581 ttttcgcttc gtcgtccgaa gaagattatg ctcctttagt ttgttttcaa taatgcccat
     2641 cgtaagtttc gtcgtataga tcggcagatt gatctctttg agcacatacg gaagcgcgcc
     2701 gatgtgatcc tcgtgaccat gtgtgataac aaaccctttc actttcgatg cattgtcttt
     2761 caagtatgtt acatcaggga tcacaagatc gatcccaagc atatcatcct ccgggaatgc
     2821 cagaccgcag tccacgacaa caatactgtc ttcatactca aaggcagtaa tattcatacc
     2881 gatctgctct aatcctccaa gcggtatgat acgcaatttt ccgttattct gttttttcaa
     2941 aaaataaaca cctccactat ttcccctttt tcatgcattc tctgcatatt ccgtaaaact
     3001 ttacttcatg atcaagaaca tgaaatccaa tcgtacgttc aatgtgacgt tccgcttctt
     3061 ccagaaggtc atcatcgaaa ggaatgattt tattgcaagt tctgcatata agatgatggt
     3121 ggttatgttt cgatccgccg tccagcagat gtccgatctc gtaacgcaca catccctcat
     3181 ccaaattaat cttgtctatg agctgcatat cccataaaag ctggatcgta cggtacacag
     3241 ttgcaagtcc gatttccgga tgctcctgcg tcaccagctc ataaatatct tccgcagtca
     3301 aatgcctgcc tttattctgc tccaagacag aaaggatcaa aattctctga ttcgtaactt
     3361 tcaacccttt ttctcttaat ttttctttga gcagttcttc tgtcctgttc tttttcattc
     3421 tatcctcacc cgaaacatcc aaaagacatt tttcactgcg atttcacgca ttttgccata
     3481 ctgtcatatc ttatatatca ctgtccgatt tcccgtggaa actctcagaa gttcccatag
     3541 catacaatgt aattttcttc ttagatcaga gttcaatact gacatcttca agaagttcct
     3601 caaacacctt ggagaccgcc tgtaactctt tctcattttc aacgatctca taaacgctct
     3661 cgcctgattc ttcatcacga acgtctttta atatcagaca ttccgcatcg tcatcctccg
     3721 actccgcaac aagaatatac gcgctcccgt tgatctttgt ttcttcaagt ataaaaaatt
     3781 cgtcctcacc gtttccatcc gcaaatgcaa actttacttt ttccatatcc gaacccctta
     3841 catcttttat ttttctatcc tgatatcctg cacaaaatca ccgaaagcgc caaaagacac
     3901 atcccgggat tgtaagcagc cgccaaggtc ttagcatatc ccgaactttg gctcattgcc
     3961 tatacaaaaa tgacagactt cattcattct gcctgttttt tcatatgcat cagatcaaga
     4021 taaccctgca aaataaacac tgcggcgatc tgatctatat gtttctttct gttttcacgg
     4081 cgcacgttgc tctcgatcag agtacgttct gcctctacgg tcgtcagtct ctcatcccac
     4141 aaaatgacct ctaacccggt tctgcgtttg agcatctctc cgaattccat agactgctgc
     4201 gcacgctctc cgatatcgtt attcatatgt ttcggaaagc cgaggacgat cttctccaca
     4261 tcatactttt tgatcagttc ctctatcctc gcgcaagtcc gccgaagttt attttcttct
     4321 tttctctcga tcgtctccac tgcctgagcc gtaagacgaa gagcgtcgct gatcgcaacg
     4381 cctaccgttt tcgacccata atcaagtccc ataattctca tatctgatta ttcccacgaa
     4441 tgatgttcga tatacgcttt taacagctcc tcgaccaact catctctctc tactttcatg
     4501 atcaggcttc tcgctccgtt aaaacttgta acatatgtcg gatctcccga catgatatat
     4561 ccgacgatct gattcaccgg attatatccc ttttctttca gcgccttaaa cacgatctcc
     4621 aaaatgtctc cggccgaaat ctgcggtccc ggttctacct gaaagaattg tgtattgctg
     4681 agatcactca tcttgctgtt cctccttacc ttctgtcttt ttgtataaga attctattcc
     4741 tcatttttca ttttcggaaa ctccgaaatc actacctaca attctaatat actcccattc
     4801 aaaattcaac gtataatttg tgaatgattt tcaattctca cttttacgat acaattagaa
     4861 atattttttt cggtttctag tgcaattttc atatactctt ttgtatgtcc tgtcataaca
     4921 gcctttccct cgatctgcgc gcgttcttca aaaagaactt ccacttcttt tccgataaag
     4981 ctgtcctcgt atgccttttt cttccgttcg ccgagggcga tcatacgggc gcttctttcc
     5041 cctttgatct tctcatcaat ctggccgtcc atcactgccg cttttgtccc ttctctcttc
     5101 gagtatttga aaatatgcgt ttcataaaaa tcaacccggt cgacaaacgc ataggaagct
     5161 tcaaactctt cctctgtctc ccccggaaat ccgacgatca catctgtcgt aagagccgga
     5221 tgatcaaaat attttctcaa tatctgacac ctgtcataat attctccgct tgtatacttt
     5281 ctgttcatcc gcttcagcgt cgcgtcacat ccgctttgaa gagaaagatg aaaatgcgga
     5341 cacactttcg gcatctcgga aagctctttt gcaaactctt ccgtaataat ccccggctca
     5401 agagaaccga gacggatccg ttcgatccct tcgactttgt gtacttcttt tataaggtca
     5461 agcagatgtc tctgcccgtc aaaatcgata ccgtaagaac tcagatggat tcccgtaaga
     5521 acaacttctt tatatccgtt tccggcaaga tctctcactt cttccagcac gtcctttgtc
     5581 tgcctgctcc gcactctgcc cctcgcataa gggatgatac aatacgtaca aaattgatta
     5641 catccgtcct gcactttaat atacgctctc gtatgatctc ccggttttgt caaatgcagt
     5701 tcttcatact ctttcgtacg gttgatatct tccatcgcat attctgtatg accgcttttt
     5761 tccatttcat attgtttcaa taattccggc agatcttttt tatgattatt tccgatcaca
     5821 atatcaatac acggatcgat ctccttaccc tcctgcgcct gtacataaca ccccgccgcc
     5881 acaacgaccg cgtccgggtt taattttctt gctcggtgca gcatctgccg agatttcctg
     5941 tctgctatat ttgtcaccgt gcatgtattg ataatataaa catccgcccc ttctttaaag
     6001 ggaacgatct cataacctgc ctgttcaagc atttcctgca ttgcttccgt ctcatatgcg
     6061 tttactttac atccaagatt atgaagtgcc gcttttttca taagtttacc tctttttcct
     6121 attctttcta tcattgttcc ttgactttta gttccctatc ccgtagaata ttcataaggt
     6181 tttgacccta ttttatcaca tatttcaagg aggtagcaaa atgaaaactg tacagatttc
     6241 attaaattca atcgacaaag taaaatcttt cgtaaatgag atcacaaaat acgacaacga
     6301 cttcgattta gtatctggaa gatacgttat agacgccaaa tccattatgg gaattttcag
     6361 tcttgatctc tccaaaccga ttgatcttaa cattcacgca gacggcgacg ttgatgcaat
     6421 cctcgctgca cttgacccgt atatcatcaa ataataacag tcaaacatat ttatgcgtaa
     6481 atgcacaatg caaaaagctg tctcttttga gacagctttt ttgtaatggc gacgagccaa
     6541 agcccgggat ataccaaaac ttcggcagcc gcttacaatc cctgaacatg ccttttggca
     6601 ctttcggtga ttctatgccc ctacggtgat cacttctgca gtttcaagcg ccgtcttcat
     6661 cagatcatcg atacaatctg ccagattgat ctccgacttt tcgattcttt ctacattcgg
     6721 ctttgtcacc ggatgctttg ccacgaaaat cgtgcagcag tcttcaaacg gctggattga
     6781 aacttcgtat gtgtcgatct tttcagatat ttccacaatc tctttcttat caaatccgat
     6841 aagcggacgg tataccggca gtgtgcagac agcgtttgtc gccgcaagac tctgcatcgt
     6901 ctgactcgcc acctgtccga tgctctctcc cgtaacaaga ccgagacagc cgtttttctt
     6961 cgcaaaatgt tctgcgatcc gcatcatata ccgacgcatg atgatcgtca gctcatcgtg
     7021 cggacatttt tcataaatgg caagctgaat atcggtaaaa ttaacaatgt gaagcttgat
     7081 cggacctgaa taagcagata cgagtcttgc aagatctatc actttttctt tcgctctctc
     7141 acttgtatac ggcggtgcgt gaaaataaac cgcctccagc tcaacgccgc gtttcgcaac
     7201 catataaccg gctaccggac tgtcgatccc tcccgacagc agaagcatcg cactgccgtt
     7261 tgttccgaca ggcattcctc ccggacccgg aatgatctcg gaataaatat aaacttcttc
     7321 tcttatctct acattcagac gtacttccgg atcgtgcaca tcaactttca tctcaggata
     7381 tgcatcaaga accgcctcgc caagcgcaca gtttaattcc atcgaattca tcggatagcg
     7441 tttattcgcg cgtcgcgctt ctactttaaa cgtcttgtct gcctgaggat acatctcgcc
     7501 tacataagca gtcacgtctt ttttcaatcc ttccggatct ttcacttctt tgcggacaac
     7561 cggacagatc cctacaatgc cgaatacttt tttgagactt tcaaccgtct cctcataatc
     7621 atattcaccc tcgcagtcca catagactct tccgtgacat ttatgaacaa ggaattctcc
     7681 gtctacgctc tgaagagcat acctgatctg acgcacaagc gcatcttcaa atatataacg
     7741 attctttccc ttgatcccta tttctccata tttaatcaaa aatgaatgaa actccatctt
     7801 cttctccttc tttgcgccat atctttctgt catgtgaaat cagcgccgtg tatatttccg
     7861 aagcatcgga atacaattat atagtgtttc caacgtatag tcaatttctt cttttgtcgt
     7921 aaactccgac aaactgaacc gcagcgtcga atcaagatat tctctcggcg ttcctatcgc
     7981 ctgaagaaca ccgcttatcg ccggatgatt agatgcacat gccgagcctg ccgacacaca
     8041 gattccacga tcttccagcg tatggagcag gacttcgctc ctcactccgg caaatcccgc
     8101 gctgacaatg tgcggagcgc ttccttcatc tgtaagtcca tgcactttca cattgccaat
     8161 ctgtgaaata cagtctataa aatgcgtttt cagttcccgc atatgctccg ttttcagctc
     8221 aagatcctga tagatcattt ttgacgccaa tgccaatcct gcgattccag gtacgttttc
     8281 cgttcctgag cgcatatctt tttgctgacc gcctccgaaa atgatcggtt ttatcttcac
     8341 acggctgtcc acataaagaa atcccgcccc tttcggtccg tgaatcttat gtccgctggc
     8401 cgaaagcata tcgactccaa gtcttttcgg atagatctta tactttccga atccttgaac
     8461 cgcatccgta tgaaataaga ttgactgttt atactgcttc acgatccggg ctgcctcagc
     8521 aacgggctgt accgttccga tctcgttatt gacatacatg accgatacaa ggatcgtctc
     8581 cggacaaagc gcttctttca gcgcgtcaag ccgaattctt ccgcattcgt caacaggaag
     8641 atacgtgacg cgaaatcctt cttcctcttc cagataatgc attgtattca aaatggcagg
     8701 atgttccaca caagaagtga tcagatgatt tccgttcctt cgattggccc gcgcacatcc
     8761 tatcagcgcc tgattatcac tttccgtacc tccggaggtg aaaataattt ctttagcatt
     8821 tacttttaaa agcttggaaa tcgtttcttt tgcttctttt atatatcttt cagcttccat
     8881 tcccttccga tacattgccg acggattccc gtaatcccga cacatgatat gatagacaag
     8941 ctctcccact tccggataac acatcgttgt agctgaatta tcaagataaa cttccatgat
     9001 tcctttcctt tactgatttc ttactattaa attatgactc ttctcttttc ggtgacacag
     9061 cgcggctttc tatatgatac atcaagatcg aaagtgtcgt aagacccgca gtctctgtcc
     9121 ttaaaatccg ctttccaagc gtgaccggcc gcgccccttt ctcttttgcc ctgagcactt
     9181 ccgcttcttc aaaacctcct tcaggaccga taaatattcc gaccgactgt cccggttcta
     9241 tttgttcgat cactctcaca gtctcggcca taccttcttc cagttcatac gggataagaa
     9301 cgacgtcaag actttctgcc tgttctaatg cttccgtaaa agaacatact ccgcaaactt
     9361 ccggaatcac gcctcttccc gactgctctg ccgctccttt tgcgattagc tgccagcgtt
     9421 cctgcttttt caccgctttc ttctcatcta actttacgac cgaacgtttt gccgaaaacg
     9481 gaacgatttt gtgagcgccc agttctaccg acttttgtac gatccattcc atcttatcgc
     9541 ctttcggaag tccctgaaac aaaataattt ccgatggaag ttccgtctcg gtatccagct
     9601 ttcggaacac atcgagcact gcctgatctt tctcataccg attcaggcag caaagataag
     9661 tcgtcccctg accgtcattg atccttacat cttctcccac tttcatgcga agtacatttt
     9721 tcatatggtt gagatccgcg cccctgatca aaatttcact ttctgtgatc catgacggct
     9781 ctgcaaaaaa ctgctgcatt cctcttctcc ttaccgtttt aattttttcg ggctgtcaca
     9841 cagacccact cgccttgata tgtaatgtca agcacttcca gacctgcttt tttcacagct
     9901 tccacaaccg tttcttcttt atcatcgata ataccgcttg taatatagat tccgcccttt
     9961 tttatctgat tcacgacaac cggagtcagc gctacaagaa cgtccgctaa aatattggcc
    10021 gcaacaatgt cataacactc atatccgact ttatcctgta cttctttgtc atcgataatg
    10081 tttccgatca tcacctcata acggtctttt gagattccgt tcacttccat attttcatga
    10141 gttgcatcga tagcacaagg atctaagtct gttccgaccg aatattttgc accgaatttc
    10201 aacgcaagca tcccgaggat cccgcttccg caccctacgt caagaatctt cgtattctcc
    10261 gttacatatt tgcggatctg gcggatgcaa agctgagttg tctcatgcat tcccgttccg
    10321 aacgccgtac ccggatcgat atggatgatc attttatctt cgtcttccgg ctttacctct
    10381 tcccatgacg gaatgatcaa aacatcgtct acataaaact ggtgaaaata ctgcttccag
    10441 ttgttcaccc agtccacatc ttccgtttcc gactcttcga tcagacactc tccgacgttt
    10501 acataagccg acatttcctc cagttctttt cttacatccg acaggatctt ctctttgtct
    10561 tcttccggtt ccaagtaaaa actcagatac gccacgccgt catccgcttc tgtctgcgga
    10621 aggatatcta caaacatctg ctccttgtcc gactgagtaa gaggaatttt atcctcgatc
    10681 tccactccct gaattccgag atccataagc atactgctta caatatcttc tgcttccgtc
    10741 gtcgtcttta accgaaattt attccatttc attttttaat cctcctgaaa ccataaattc
    10801 aaatctacat ttcccgtgat accgggaact gcgcccgttt cactgtactg ccatatttta
    10861 tattcatacg gacactgcgg cgtctcatga taatccgcat accagaaatc atactctgta
    10921 agctgctcca tatcaagcgt aaaagtcatc cattttaaat ttgcgtaaat catcggatcg
    10981 tatcccgcat gttcgatcgt atcacaaaac actttgcaat aattcgtaaa atcctgccgc
    11041 gtatttccat ctgttctcgc gctgtcccac ttgatctctt ctgtatcaaa aacaacagga
    11101 cccgtaatat tataatgctt caaatgctca agcacaaatt ccgcttcctc cactgcctca
    11161 gccggagaaa ctgcctgtga aaaaaaatac acgcctacct gcagccctgc ttccagagcg
    11221 ttcttcacgt tttgctcgta catcgcatcc aatacaagag agccgctctc tccgtaagcc
    11281 cgatacccga gacgaaccat aacaaattcg acaccggatt ctttcacctg cttccagtcg
    11341 atcacttctc cttgaaattc agatacgtcg atcccaagtt ttgcctttgc acctgtttcc
    11401 gggtcctgat agcttttata acctgtctgc tcatctgtct ttaaatcgga aaaatcgtaa
    11461 gtacatttcg gaacatccga aagcagcggc gcttcataac ggttctcttc cacatcttga
    11521 aaacagtatc gcttcttttc cccgtcctga ttgccgcctg cattttgtat gagagaaccg
    11581 ttcttctgaa caatccactc tcccgaatcg tatgttttat cctcatttcc tgttacagac
    11641 tgcgaaatca cctctttttt cggagaacag gctgccagca taagaatcgt acagcttaca
    11701 accgcaatca tctttgccgt ttttctcata cttattttta ttttcagact cgtcctctcc
    11761 ccgttttcat ctgt
//
LOCUS       DBMDXF010000045        11647 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1571_length_11647_cov_2.295860, whole genome shotgun sequence.
ACCESSION   DBMDXF010000045 DBMDXF010000000
VERSION     DBMDXF010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11647)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_11647_cov_2.295860"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..1256)
                     /locus_tag="ACZPD4_03150"
     CDS             complement(63..1256)
                     /locus_tag="ACZPD4_03150"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1378106.1"
                     /translation="MEMYKRIFVIVLDSLGIGAMPDAARFADEGADTFGHILEKTKTL
                     NIPNLRRLGFLNLKCGGDMKGVESPEGRFMRMREASNGKDTMTGHWEMMGIKTEKPFR
                     TFTEHGFPAELIKELEKQCGKKVIGNKSASGTEIIEELGEEEIRTGSMIVYTSADSVM
                     QICGNEETFDLEELYRCCETARRITMKDEWRVGRVIARPYIGMKKGEFKRTSNRRDYA
                     LKPPAPTVLNALKDAGNDVIGVGKIHDIFCGEGITKTYHSESSVHGMEQTIDVCKEEF
                     TGLCFVNLVDFDALWGHRRNVEGYGKEIEKFDEKLGVLLECLSQDDLLILTADHGNDP
                     TYKGTDHTREYVPFLAYSKRMQAGGPLPEAETFAVIGATVAENFGVDMPKGTIGYSVL
                     SRLQQ"
     gene            complement(1332..2429)
                     /locus_tag="ACZPD4_03155"
     CDS             complement(1332..2429)
                     /locus_tag="ACZPD4_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR1378107.1"
                     /translation="MAAKKIGISRDLIIHPGETIADVLEERGITQSELASSAGVSPAY
                     VSNVIAGKKGISANFARGLEYAIGVPKSFWLNLQANYEAELLEANELQTITEEERIVR
                     EDLKEIVKYFRERGMMPSRENKDDSILSLRKILQISNLVNLNEIIPGGAFRMSGNLSV
                     NRNVLGAWIRLCQILENNQNVSTKFEVEHINELIGKIKNVMCQKNVDFQNHLKTVLGE
                     YGIDFSVVKNFRGAPVQGYIFRRSDGVYQMVLTIRGAFADIFWFSLFHELGHIVNGDI
                     GKSIKFIDDGSDVDKETAADLFARDRLLSPESYASFIQKGDFTFEAICSYARTQNVMP
                     YIVIGRLQKEKYLDYHQYSNYKLRYKWGDCS"
     gene            complement(2398..2736)
                     /locus_tag="ACZPD4_03160"
     CDS             complement(2398..2736)
                     /locus_tag="ACZPD4_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378108.1"
                     /translation="MEITYANGKVEKYFKDYKKMQKKLPFEWVKTIKKHMDRLKAAEC
                     FGDFLKLRLGKPEQLEGCESIRYSLHVAANVRLIIEPNAEQDTVMICTEIEVEGVSDY
                     HGSKENWYIP"
     gene            complement(2810..3232)
                     /gene="cdd"
                     /locus_tag="ACZPD4_03165"
     CDS             complement(2810..3232)
                     /gene="cdd"
                     /locus_tag="ACZPD4_03165"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR1378109.1"
                     /translation="MELSQGMIAKLIETATAQLAYSYSPYSGFKVGAALLAESGRIYT
                     GCNIENSAFSPTNCAERTAFFKAVSEGERKFRAICIIGGKDISETVCTPPCGVCRQVM
                     AEFCDPKKFKVILASGREKYRILRLEELLPFGFGSEYL"
     gene            complement(3260..3910)
                     /gene="deoC"
                     /locus_tag="ACZPD4_03170"
                     /pseudo
     CDS             complement(3260..3910)
                     /gene="deoC"
                     /locus_tag="ACZPD4_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(4112..4894)
                     /gene="udp"
                     /locus_tag="ACZPD4_03175"
     CDS             complement(4112..4894)
                     /gene="udp"
                     /locus_tag="ACZPD4_03175"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR1378110.1"
                     /translation="MKNYSEDPNRQYHIQTAKGEIGKYVIMPGDPKRCVKIAQYLDNP
                     VLIADNREYVTYTGTLDQVKVSVTSTGIGGPSAAIAMEELCRCGADTFIRIGTCGGMQ
                     TDVKSGDIVVSTGAIRMEGTSREYAPIEFPAVPDLTVTNALVKAVKSKGYPFHTGVVQ
                     CKDSFYGQHEPERKPVGYDLLHKWEAWKQLGCLASEMESAALFVVASSLGVRVGSCFL
                     VIANQEREKAGLENPVVHDTDMAIRVAVDAIRRLISEERKKE"
     gene            complement(5079..7157)
                     /gene="glgB"
                     /locus_tag="ACZPD4_03180"
     CDS             complement(5079..7157)
                     /gene="glgB"
                     /locus_tag="ACZPD4_03180"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1378111.1"
                     /translation="MARRKGAGRNAAKSAGKGIVNHAAGGMAGSAVKKASGGASRNRA
                     NSPAKNTAADAASMKANEHAYEIGELDHYLFGQGNHYEIYKKLGAHKVVNDKTEGVYF
                     AVWAPNAKNVSVVGEFNDWNLERDRMERQEPLGIYVKFVPEAKEGQLYKFCIETQKGE
                     LLYKADPFANYAELRPGTASRITDLSRIRWTDDAWMKARKTWNHKKNPMSIYEVHMGS
                     WMRHPGREDEGFYTYREFAEAIVKYVKEMGYTHVELMGIAEHPFDGSWGYQVTGYYAP
                     TSRYGSPEDFAYLVDLLHKNNIGVILDWVPAHFPKDAHGLADFDGTPTFEYADPRLGE
                     HPDWGTKVFDYGKAEVRNFLIANALFWIEHFHVDGLRVDAVASMLYLDYGKQDGQWVA
                     NKYGGNENLEAIDFFKHLNSVVLGRNKGAVMIAEESTAWPKVTGSPEDDGLGFSLKWN
                     MGWMHDFTEYMKLDPYFRKNAHHMMTFAMQYAYSENYILVLSHDEVVHLKCSMINKMP
                     GLGFDKFANLKAGYAFMMGHPGKKLLFMGQEFAQLREWSEKREIDWFLLAEPEHRDIQ
                     NWYRDLLHLYKNNKALYEMDNDPAGFEWINADDIFRSIYSFVRHSKNKEKNLLFVCNF
                     TPVEREDYRVGVPTRKTLKLVLNSDEEKYGGKGEKRPLTYKPVKKECDGQAYSIAYPL
                     PAYGVAVFEY"
     gene            complement(7345..11124)
                     /locus_tag="ACZPD4_03185"
     CDS             complement(7345..11124)
                     /locus_tag="ACZPD4_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378112.1"
                     /translation="MDKYEYKSKTERMLELMESGAYSRAAAIADEIDWRRVRNAVMLS
                     NVSEIYEKTGEYQKGYDILTLAYQRAEGSRKIISRLCGLALKTGNVDEAIDFYDEFMQ
                     IAPKDPNQYILRYKILRAQRAPIEQQIEALEEYKKSEYIEEWAYELAKLYQEAGMTSE
                     CLEECDDLILWFSEGQYVYKAMELKMQYKPLTPSQQEKYDKRYARTSEETEEIPDIFS
                     YAEADESEQEEEENGLPGAELMAADSEVADADFAAEQQVNARVPKGMEELVPDMMPET
                     ISEGVREITLPNDPVEVDTEAWEADEIKALEADSPAEDAEDHVEQDEQVPIDAEDAGQ
                     TEDAYLPTDVSEAEGVDEPEERVPEGIKGPDVLETTAEPEEERQPAKKKLGDTMPLDV
                     ALRKLLHPELEELTADDFQDETAPMDFNFEDEEESESDLTETGAMDFDFEDEEEPEFD
                     LTETGAMDFDFENEEEPEFDLTETGAMDFDFEDEGEPEFDLTETGAMDFDFEDEEESE
                     FDLTETGAMDFDFEDEEESEFGKEKDMGNTSQIKFRPVRKDEIEDEEDLADLEQAIGE
                     IESVADIDMVSRLKEEKRRAALQAEEEEKAEEAARAEKAAQAEEAARAEEAAQAEEAA
                     QAEEAAQAEAAAEIKEDDQITGQISLEDILSGFEENAGEDEPVEEGTDAEGASESEQE
                     EVIDSAGDTAKGDTMETNKKTTPILPPDIQRMIDEIEGIIPPEEEKKEPTGVYLKRKE
                     EPSENMGQVAEDLRIDDDFDDDFDEDFDEEFEEDLDDDYFEEDYYEEADEVIEEPEED
                     YYEESEEVIEEPEEDYYEEADEVIEEPEEDYYEESEEVIEELEEDYYEESEEVIEEPE
                     EDYYEESEEVIEEPEEDYYEESEEVIEEPEEDYYEEADEVIEEAEGNADDEYFFGEDD
                     AEEIQDIEDEFTVNPDYSGEDDDREIPDDDEPIDIMSATTPLSRKETAKLIATGKTAP
                     LPLDEISDALSMDTGFVVHGRYDLEMQSGIGTRAGLTEEQKKLFSYFVPVRGMSEQLV
                     DVLEQDKNCTNRKGTSRTGNLLIIGNKGNGKTVLAVDVVKAIQKQRDIRQGKVAIVTG
                     DSLNKKKISDIVEKLYGGALIIEKASKMNEKTVARLNKAMEQDTGEMLIVLEEQRKPL
                     DRLLSSNREFRKKFTSRLEVPIFINDELVTFGQTYAKENGYHIDEMGILALYSKIDSL
                     QREDHFVTVAEVKEVMDDAIRHSQKASAKKLMRKVFGRNTDESDRIILSEKDFNI"
     assembly_gap    11265..11274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2659 a         3359 c         1840 g         3779 t
ORIGIN      
        1 tttacatttt tacagttttt catcctactt ccgctttgat ttttttacat tttcacggtt
       61 tactactgct gcagcctgct taaaacagaa tatcctatcg ttcctttcgg catatctact
      121 ccgaagttct ctgctacagt cgcccctatc accgcaaacg tctctgcttc cggaagcgga
      181 cctcccgcct gcattctttt gctgtatgca aggaaaggaa catactctct cgtatgatcc
      241 gttcccttgt atgtcggatc attcccgtga tctgcggtca ggatcaatag atcatcctgc
      301 gaaagacatt ccagcaaaac gccgagtttc tcatcaaact tttcgatctc tttgccatac
      361 ccttctacat ttctccgatg cccccataat gcgtcaaaat caacaaggtt tacaaaacac
      421 agtcccgtaa attcctcttt gcacacatcg atcgtctgtt ccatcccatg aacggaactt
      481 tccgaatgat atgttttcgt aatcccttcc ccgcagaaaa tgtcatgaat ctttcccacg
      541 ccgatcacat cattccccgc atctttcaga gcatttaaaa cagtgggcgc cggcggcttc
      601 aacgcgtagt ctctccgatt gctcgtcctt ttaaactcac cttttttcat tccgatatac
      661 ggtcttgcga tcacccttcc tacgcgccat tcatctttca tcgtgatccg ccttgcagtc
      721 tcacagcacc gatacagttc ttccagatcg aaagtctctt catttccgca gatctgcata
      781 accgaatccg ccgacgtata cacgatcata gatcctgtac gaatctcttc ctcgcccagc
      841 tcttctataa tctcagtacc gctcgcgctt ttatttccga tcaccttttt cccacactgt
      901 ttctccagct ccttgatcaa ttctgccgga aatccatgtt ccgtaaaagt ccgaaacggc
      961 ttttccgttt taatccccat catctcccag tgtccggtca tcgtatcttt tccgttgctc
     1021 gcttcccgca tccgcatgaa gcgcccctcg ggagactcga cgcctttcat atctccgccg
     1081 cactttaagt tcagaaaccc aagcctgcga agattcggaa tattcaaagt ttttgttttc
     1141 tccaaaatat gtccgaacgt atccgcgcct tcatctgcga accgagccgc atccggcatc
     1201 gccccgatcc cgagagaatc aagaacgatt acaaatattc ttttatacat ttccattatc
     1261 ccatctccct gtacaacact cttatatctt gcccttattc ccaataaatc aatactgtat
     1321 ccactttatt cttatgaaca gtctccccat ttataccgca atttgtaatt gctatactga
     1381 tgataatcca gatatttctc cttttgaagt cttccgataa caatgtaagg catcacattc
     1441 tgagtccgtg cataactaca gattgcttca aatgtaaagt ctcctttttg gataaaactt
     1501 gcataactct ccggtgacaa aagtctatct ctcgcaaaaa gatctgctgc cgtctcctta
     1561 tccacatcac tcccgtcatc tataaattta atacttttac ctatatctcc atttacaata
     1621 tgtcccagtt catgaaataa agaaaaccaa aaaatatccg caaacgctcc ccttatcgtc
     1681 aaaaccattt gatacacccc atcgctccgc cgaaaaatat acccttgcac aggcgctcct
     1741 ctaaaatttt tcacaacaga aaaatctatg ccatattctc ccaatacagt tttcaaatga
     1801 ttttgaaaat ccacattctt ctgacacata acatttttta ttttacctat caattcgttt
     1861 atatgctcta cttcaaactt tgttgaaaca ttttgattat tctccaaaat ttgacacaaa
     1921 cgaatccatg cccccaacac atttctattt accgataagt ttccggacat ccgaaatgct
     1981 cccccgggaa ttatttcatt taaatttacc aaattactaa tctgcagaat tttacgaaga
     2041 gaaagtatcg aatcatcttt attttcacga ctaggcatca tgcctctttc tctaaagtat
     2101 ttcacaattt cttttaagtc ttccctcacg atacgctctt cttccgtaat cgtctgtaat
     2161 tcatttgctt ccaaaagttc cgcctcatag ttcgcctgaa gattcagcca aaaagacttt
     2221 ggtactccga ttgcatactc taatcctctg gcaaagtttg cagatatccc tttctttccg
     2281 gcaataacat tgcttacgta agccggagaa acgcctgcac tcgacgccag ttcactctgt
     2341 gtgattccac gttcttccaa gacgtctgca atcgtttctc ccgggtgaat tattaaatca
     2401 cgggatatac caattttctt tgctgccatg ataatcactc actccttcca cttctatttc
     2461 cgtacaaatc ataaccgtat cctgttcggc attcggctcg ataatcaatc ttacatttgc
     2521 cgctacatgc aacgaatatc gaatactttc gcatccttct aactgttcag gctttcctaa
     2581 tctcaatttc aaaaaatctc caaaacattc cgccgctttt agtctgtcca tatgcttttt
     2641 tattgttttc acccactcaa aaggcagttt cttttgcatc tttttataat ctttaaagta
     2701 tttttcaact tttccattcg catatgttat ttccactcaa tacatcaccc tcacattaac
     2761 cttttggtta atttattatg tcatgttttc tttttcttgt caatcgttcc tacagatact
     2821 ccgacccaaa cccaaacggc aaaagttctt ccaaccgaag gattctatac ttttccctgc
     2881 ctgatgccaa tatcaccttg aacttcttcg gatcgcaaaa ttctgccata acctgacggc
     2941 acactccgca aggaggcgta catactgttt ctgaaatatc ttttccaccg atgatgcaaa
     3001 ttgcgcgaaa cttcctttct ccttcgctca ccgccttaaa aaacgcagtt ctctccgcac
     3061 aatttgtcgg tgaaaatgca gaattttcta tattgcatcc ggtatagatc ctgccgcttt
     3121 cagccaaaag agctgcgcct actttaaatc ccgagtaggg actgtatgaa tacgccagtt
     3181 gtgcagtcgc tgtctcaatt aactttgcga tcattccctg cgataattcc atttcacaag
     3241 ttccttccgc cgggttttct tcttccgttt tcacgatttt tacaatgcgg ctcgttccga
     3301 gtctgtctgc cccaagctcc agaaactttt ccgcgtcttc catcgaggaa atgccgcccg
     3361 ccgctttcat cttcacgtta ccgccgacat gatccgcaaa caggctgata tcgtcaaaag
     3421 tcgcgcctgc ttttgaaaaa cctgtggacg ttttaatata atccgctccc gaacgagtga
     3481 cgatctcaca cattttcact ttttcttcat ctgtcaaaag acatgtttct atgatcactt
     3541 taagaacttt actgccgcac gcatttttca gaatgcggat ttcttcttcg atctgatcgt
     3601 attttctgtc tttcagccat ccgatattga taaccatatc gatctcatcc gctccgtttg
     3661 cgatcgcatc ttttgtctca aattccttta cggcagtcgt ctcgtaaccg ttcggaaaac
     3721 cgatcacagt acaaatcgga actcttccgc ctacatattc cgccgcttgc ttcacataac
     3781 ttggaggaat acagactgac gccgtatcat atttcaccgc atcatcacat acctgacgaa
     3841 tctcatccca cacagcttcc tgcgacagca atgtgtgatc cacatgccgt aaaatttctt
     3901 tttcattcat atccttcatt tgaccgttta tttcctctct ttattttccg catttatttt
     3961 ctttcactta tttttcccgc tttttcccct catttctttt ctcgcgttta ttttctttca
     4021 tttatttttc ccgcttatct cccccattca tttttccgtt tttcttttct cccgctcttt
     4081 tcttctcgtt gtcattttct ttatatgcat tctactcttt tttccgttct tcggaaatca
     4141 atctgcggat cgcgtccact gccacccgga tcgccatgtc cgtatcgtgc actaccgggt
     4201 tctcaagtcc tgctttctca cgttcctgat tcgcgatcac aagaaaacac gagccgaccc
     4261 gcaccccaag gctgctcgcc acaacaaaca gagccgccga ttccatttcc gaggcaagac
     4321 agccgagttg tttccacgcc tcccacttat gaagcagatc gtagcctacc ggctttctct
     4381 ccggttcatg ctgaccgtaa aacgaatctt tgcactgtac aactcccgta tggaacggat
     4441 atccttttga tttaacagct ttcacaagcg cattcgtcac tgtcagatcg ggcactgccg
     4501 gaaattcgat cggcgcatac tccctgctcg ttccttccat cctgattgcc cctgttgaca
     4561 caacgatatc tccgcttttc acatctgtct gcattccgcc gcaagttccg atcctgataa
     4621 acgtatccgc accgcaccta caaagttctt ccattgcaat agctgccgac ggtcctccta
     4681 ttcccgtaga tgtaacgctt accttcactt gatcgagcgt tcctgtatat gtcacatact
     4741 ctctgttatc tgcaatcaac accgggttat ctaaatattg cgcgatcttc acacatcttt
     4801 tcggatctcc gggcataatg acgtacttcc cgatttcccc ttttgcagtt tgtatatgat
     4861 actgtctgtt cggatcttcc gaataatttt tcatctgctg ttcctcctca ctttgccggt
     4921 aaaaacaaaa ccgccgcaaa acagacaggc tattttaaaa atcccccatc tattttgcgg
     4981 cagtttgctc tgcatttttt accttttata taatagtgaa ccgcccaccg gttaaatcca
     5041 atggggttcc ggtttcaagt cagaagctgc ctgacatctt aatactcaaa cactgccaca
     5101 ccataggccg gaagcgggta tgcgatcgaa tacgcctgac cgtcgcattc ctttttcacc
     5161 ggtttgtaag taagcggacg tttttcccct tttcctccgt acttttcctc atcactgttc
     5221 aaaacaagct tcagcgtttt cctcgtcgga acgccgactc tgtaatcttc tctttccaca
     5281 ggagtaaagt tgcagacgaa aagcaaattc ttttctttat tcttggaatg tcttacaaag
     5341 ctgtaaatac ttctgaaaat atcatccgca ttgatccact caaatcccgc cggatcattg
     5401 tccatttcat aaagagcttt attgttttta tacaaatgaa gcagatcccg ataccagttc
     5461 tgaatatctc tgtgctcagg ttcggcgagc aggaaccaat cgatctctct cttttcgctc
     5521 cattctctaa gctgtgcaaa ttcctgaccc atgaacagaa gtttctttcc cggatggccc
     5581 atcataaacg catagcctgc ttttaggttg gcaaatttat cgaacccgag tcccggcatt
     5641 ttattgatca tcgaacactt cagatgaaca acttcgtcat gcgacaacac aagaatataa
     5701 ttttcactgt atgcatactg catcgcaaac gtcatcatat gatgtgcatt tttacggaaa
     5761 tacggatcaa gtttcatgta ctccgtaaaa tcgtgcatcc atcccatatt ccatttcagg
     5821 ctgaaaccaa ggccgtcgtc ttcgggcgat cctgtcacct tcggccatgc cgtcgattct
     5881 tccgcgatca tcacagcgcc tttgttccgt cccagaacaa ccgaattcaa atgtttaaag
     5941 aaatcgatcg cctcaagatt ctcgttgcct ccgtatttgt tcgcgaccca ctgtccgtcc
     6001 tgttttccgt aatcaagata gagcatcgac gcaacagcat ccacacggag cccgtccaca
     6061 tggaaatgtt cgatccagaa taatgcgttt gcaatcagga agttgcgcac ttccgctttt
     6121 ccgtaatcaa atactttcgt tccccaatcc ggatgttctc cgagtctggg atctgcatac
     6181 tcgaatgtcg gagttccgtc aaaatcagca agcccgtgag catctttcgg aaaatgcgcc
     6241 ggaacccagt caagaatcac tcctatattg tttttatgca gaaggtcaac cagatacgca
     6301 aaatcttcag gacttccgta tcttgaagtc ggcgcataat atcccgtaac ctgatatccc
     6361 cacgaaccgt caaacggatg ctctgcaatg cccatcagtt ctacatgagt gtatcccatc
     6421 tcttttacat attttacgat cgcctcggcg aactctctgt atgtgtagaa tccttcatct
     6481 tcccttcccg ggtgacgcat ccacgaaccc atatgaactt catagatcga catcggattc
     6541 ttcttatgat tccatgtctt ccgcgctttc atccatgcgt catccgtcca tcttatacgg
     6601 gaaagatccg taatacgaga cgccgttccc ggacgaagct ccgcataatt tgcaaacgga
     6661 tccgccttat aaagaagctc gcctttctgc gtctcaatac agaacttata gagctgtcct
     6721 tctttcgctt ccggtacaaa cttcacataa ataccgagag gctcctgacg ctccattctg
     6781 tctctttcca aattccagtc attaaattcc ccgactacag acacattctt cgcattcggc
     6841 gcccataccg cgaaataaac gccctccgtt ttgtcgttca ctactttatg tgcgcctaat
     6901 tttttataga tctcgtaatg gtttccctga ccaaacaggt aatgatccaa ttctcctatc
     6961 tcataggcat gttcattcgc tttcatactt gccgcatccg ccgcagtgtt tttcgccgga
     7021 ctgttcgccc tgttccggct tgcgccgcct gacgcctttt tcaccgcact gcccgccata
     7081 ccgccggctg catgattcac gatgcctttg cctgcacttt ttgccgcgtt tctgcccgca
     7141 ccttttctcc ttgccataaa agcccacctc gccgtttcct cataataaaa tatcgaagcg
     7201 tttctgcccg cttccgtgat agtttcatta tacttgattc cgtccaaaaa ataaagagaa
     7261 acagacagct ctttcccgcc cgtttctctt gtattttgtc tatttatgca cacattttaa
     7321 attatagtct attatcgtgc ttctttatat attaaaatct ttttcagaaa gaataatacg
     7381 gtctgactcg tctgtatttc ttccgaaaac tttccgcatc aatttcttcg cggacgcctt
     7441 ctgagaatga cggatcgcgt catccataac ttctttcact tccgcaaccg tcacaaaatg
     7501 atcttctctc tgcagagaat caattttact atacagcgca agaattccca tctcgtcaat
     7561 gtgatatccg ttttctttcg catacgtctg accgaacgta acaagctcat cgttgatgaa
     7621 aatcggcact tccagtcttg atgtgaattt tttcctgaat tctctgttcg atgacaacag
     7681 tctgtcaagc ggctttctct gctcttccaa gacgatcagc atctcgcctg tatcctgctc
     7741 catcgcttta ttaagacgcg caactgtctt ttcattcatt ttggacgctt tctcaatgat
     7801 cagagcgccg ccgtaaagtt tttcgacaat atcgctgatc ttcttcttat tcagagaatc
     7861 tcctgtcacg atcgcgacct tgccctgacg gatgtctctt tgcttctgga tcgctttcac
     7921 gacgtcaacg gcaagaaccg tttttccgtt gcctttattt ccaatgataa gcaagttgcc
     7981 cgttctcgaa gtcccttttc tgttcgtaca gttcttatcc tgctcaagca catcgacaag
     8041 ctgttcgctc attccgcgca ccggaacaaa gtaagaaaac agtttcttct gttcctctgt
     8101 aagtccggct ctcgttccga taccgctttg catctcaagg tcatatcttc catgtacgac
     8161 aaaaccggta tccattgaca atgcatccga gatctcatcg agcggaagcg gcgcagtctt
     8221 acctgtcgca atcagcttcg cagtctcttt acggctaagc ggtgtcgtcg ccgacataat
     8281 gtcgatcggc tcgtcatcat caggaatctc tctgtcgtca tcctctcccg aataatcagg
     8341 atttactgtg aactcatcct caatgtcctg aatctcttcc gcgtcatctt ccccgaagaa
     8401 atactcgtca tctgcgtttc cttccgcctc ttcgatcact tcatccgctt cttcgtagta
     8461 atcttcttcc ggttcttcaa tcacttcttc tgactcttca tagtaatctt cttccggctc
     8521 ttcgatcact tcttctgact cttcatagta atcttcttcc ggttcttcga tcacttcctc
     8581 tgattcttca tagtaatctt cctccagttc ttcgatcact tcttctgatt cttcatagta
     8641 atcttcttcc ggctcttcga tcacttcgtc tgcttcttcg tagtaatctt cctccggttc
     8701 ttcgatcact tcctctgatt cttcatagta atcttcttcc ggctcttcga tcacttcgtc
     8761 tgcttcctca tagtagtctt cttcaaagta atcgtcatcc aaatcttcct cgaactcctc
     8821 gtcgaaatct tcgtcaaagt catcatcaaa atcatcgtcg attctaagat cctccgcaac
     8881 ctgacccatg ttttcgcttg gttcttcttt tctcttaaga tatactcctg taggttcttt
     8941 cttttcttct tccggcggga taattccctc aatttcatca atcatacgtt gaatatccgg
     9001 cggtaaaatc ggtgttgttt tcttattcgt ctccatagta tccccttttg ctgtatcccc
     9061 tgctgaatct ataacttctt cttgttccga ttcggatgct ccttctgcat ccgtcccctc
     9121 ttcaaccggc tcatcctctc ctgcattttc ttcaaatccc gaaaggatat cttccaaact
     9181 gatctgacct gtaatctgat cgtcctcttt tatttcagcg gccgcttccg cttgcgctgc
     9241 ctcctccgct tgtgcggctt cttctgcttg tgccgcctct tccgctcttg cggcttcttc
     9301 tgcttgtgcc gccttttctg ctcttgcggc ttcttccgct ttctcctctt cttctgcctg
     9361 taatgcggcg cggcgttttt cctctttcaa acgacttacc atgtctatat ctgcaaccga
     9421 ctcaatctct ccgatcgcct gctccagatc cgcaagatct tcttcatcct cgatctcatc
     9481 ttttcttacc ggacggaatt tgatctggga tgtatttccc atatcctttt ctttgccgaa
     9541 ttccgactcc tcttcatcct caaagtcaaa atccatcgct cctgtttccg tcaaatcgaa
     9601 ctccgactct tcttcgtctt caaaatcaaa gtccatcgct cccgtttccg tcaagtcaaa
     9661 ctccggctct ccttcatctt caaagtcaaa gtccatcgct cccgtttccg tcaagtcaaa
     9721 ctccggctct tcttcattct caaagtcgaa atccatcgct cccgtttccg ttaaatcaaa
     9781 ctccggctct tcttcatctt caaagtcaaa gtccatcgct cccgtttccg tcaaatcaga
     9841 ttccgactct tcttcatctt caaaattaaa atccatcgga gctgtctcgt cttggaaatc
     9901 atctgccgtc aattcttcca actccggatg aagaagtttt ctcaatgcaa cgtctaacgg
     9961 catcgtatct cccagtttct ttttagcagg ctgcctttct tcttccggct ctgcggttgt
    10021 ctccagcaca tccggtcctt ttattccttc cggtacgcgc tcctccggct catcgactcc
    10081 ttctgcttcg gaaacatccg tcggcagata tgcgtcttct gtctgcccgg catcttccgc
    10141 atctatcggc acctgctcgt cctgttcaac atgatcctct gcatcctccg ccggtgaatc
    10201 cgcttctaac gccttgatct cgtcggcttc ccacgcttcc gtatctactt ccaccgggtc
    10261 gttcggcaaa gtgatctctc ttactccttc tgagatcgtt tccggcatca tgtccggaac
    10321 aagttcctcc attcccttcg gaactcgtgc gttcacctgc tgttcggcag cgaaatcagc
    10381 atcggcaact tcggagtcgg cagccataag ttcagctccc ggcaaaccgt tttcttcttc
    10441 ctcctgctcg gattcatctg cctctgcata agaaaaaata tcaggaatct cctctgtctc
    10501 ctcggacgtc cttgcataac gcttatcgta cttttcctgc tgggaaggag tcagtggttt
    10561 gtactgcatc ttcagttcca tcgccttata gacatactgc ccctcgctga accaaagaat
    10621 caaatcgtca cactcttcca gacattctga tgtcataccg gcttcctgat acagcttcgc
    10681 cagttcatat gcccactctt caatatattc ggatttctta tattcttcaa gagcttcgat
    10741 ctgctgctcg atcggcgccc tctgcgcgcg gagaatctta tatcttaaaa tgtactgatt
    10801 cgggtctttc ggcgcgatct gcataaactc atcgtaaaaa tcgatcgcct catcgacatt
    10861 tcccgtctta agagccaatc cgcacagacg gctgataatc ttacggcttc cttccgcccg
    10921 ctgatatgca agcgtcaaaa tgtcatatcc cttctgatat tctccggttt tttcataaat
    10981 ttcactgacg tttgacaaca tcactgcatt gcgcacgcgc ctccagtcaa tttcatctgc
    11041 gatcgccgcc gctctgctgt acgcgccgct ctccataagt tcgagcatcc gttcagtctt
    11101 tgatttatat tcgtatttat ccaccgcatt cacctcatta aaaaatagtt ttgtacagag
    11161 cagtgtcttt ccttctatgt ttctttacat actatatctt tcaatatttt atcatatgca
    11221 tttcgcaatg tcaaaaaaat gcaccctcaa ttaacattga agatnnnnnn nnnnctcttt
    11281 atttttaaat cggaggcaaa tattagattc tgttcctgta agacctctgc cattgttatt
    11341 tgatctgcgg tcgatttatt aagacctgct tattaaatct tccctccttc aattaacaaa
    11401 ttcgagttca taacagtatc cttatcacat atcacttacg ttccttgttt ccggtaccac
    11461 cacacatatt aaacccgata tgcacgaatg ataatctact caatgtttct ttacacatct
    11521 tttgaaattc tgctatatgt ggtatctata ttcaatatgt ctgcaaatgt taaatctttc
    11581 tgatctctat ccggacttcc aactctccga tcaattcttt cgttattttt attccggatc
    11641 atctatc
//
LOCUS       DBMDXF010000046        11311 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1629_length_11311_cov_2.018912, whole genome shotgun sequence.
ACCESSION   DBMDXF010000046 DBMDXF010000000
VERSION     DBMDXF010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1629_length_11311_cov_2.018912"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(197..1153)
                     /gene="cbiB"
                     /locus_tag="ACZPD4_03190"
     CDS             complement(197..1153)
                     /gene="cbiB"
                     /locus_tag="ACZPD4_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR1378113.1"
                     /translation="MMSLAACVTGFFLDRLFGDPVWLYHPVRMIGNMISFLEKRLRKE
                     NDKKNMTAGGVLWIIVAILSFGIPFAGLRVAQNIHPLICFFLESFWCYQIFAAKSLSE
                     ESKKVYYCLKRNDMKEARRAVSMIVGRDTENLTEEGVTKAAVETVAENTSDGVTAPLL
                     YMMLGGAPLGFLYKAVNTMDSMLGYKNEKYLYFGKIPAKMDDVFNFIPARVTAMFMVC
                     ASFLAGLDGKNAWRIYLRDRRKHASPNAAQTESVCAGALRIRLAGDAVYFGKVYKKEF
                     IGDDLRPIDPEDIIKAGRLMYVTALLMMFVFGGIKLCTVLIM"
     gene            complement(1150..1755)
                     /locus_tag="ACZPD4_03195"
     CDS             complement(1150..1755)
                     /locus_tag="ACZPD4_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1378114.1"
                     /translation="MLKIMLIRHFATPGNEKRQYIGSTDEVLSEKTAFLKRSYPIPEY
                     IIASPMKRCVQTAKRIWGTDLSGCPIVLEPLMRECDFGQFEGKTYEQLKKNAAYIKWL
                     KSGGITAFPGGEDQEEFRKRCVEGMKKQVRFLIARHVKYAAFVVHGGTIMAVMHALYE
                     EKKDFYNWQVENGSGYLVSVSEKEWENGIELLKNAEKRSGR"
     gene            complement(1749..2513)
                     /gene="cobS"
                     /locus_tag="ACZPD4_03200"
     CDS             complement(1749..2513)
                     /gene="cobS"
                     /locus_tag="ACZPD4_03200"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR1378115.1"
                     /translation="MNMLKSLIIALAMYSKLPVPNIDWDEKNMRYAMCFFPVVGVIIG
                     ALVFGAGQLILRYTECGKLFFAAVMTMIPVAVTGGIHLDGFADTIDAVSSYKDREKKL
                     EILKDPHTGAFAVIGLCAYFLITAALWSEVNLKTLSLAVWMYPISRALSGLSVVSFRS
                     AKSSGLLRTFQERADRKRTRIVLSIWLMSLAAGAIYIFRVQGAIIVTAALLVFFYYYK
                     MSFKQFGGITGDLAGYFLQICELVMLTVVAFLGGIC"
     gene            complement(2565..5165)
                     /locus_tag="ACZPD4_03205"
     CDS             complement(2565..5165)
                     /locus_tag="ACZPD4_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HIR1378116.1"
                     /translation="MASGILITAISSGSGKTAAACALMAAYQAQNKKVSSCKCGPDYI
                     DPMFHKEVLGVDSKNLDLFFSSEDELRKEYAKHAADAELVITEGVMGYYDGMRLDSVT
                     ASSYDVARTLDIPAVLILPCKGAALSLCAVVSGIVSFQKDSNIQGIILNRVSKMLYPR
                     LKKMLEDHLKSEGYDIPVLGYIPQDEAFCLESRHLGLVTPQEICHLKEQMTKAGNIVT
                     ETVDLDRLYQIAQGEQKANSPEKGQIKQNMRNGQSREPFQCDMEKYKSSDDGKVRVGV
                     AKDAAFCFYYKENLELLEESGAELVFFSPLRDQKIPENISGLIFGGGYPELNCKELSE
                     NNSMRRSVKDAIRSGMPCLAECGGFMYLHEEMEDERHIVWEMAGVLNGRTYPAGKLVR
                     FGYVELSHEKEQKESCYLKQGEVIKGHEFHYWDSSDNGEGLTAAKPDRRTSWKCVHTE
                     GSLFAGYPHLYMPSCPQFAKRFTDQCRLFAKENEANKKKQRRNHMSEDRKNMKEQSEP
                     ELEKVTKRLNEYLEQICPPDQKAAAQAKKRWKQIAKPLFSLGKLEDAVTKIAGMKGSP
                     AYSLDKKGLVIMCADNGVVEEGVTQTGQEVTAVVAENFTKSETSVCKMAQIAGVDLFP
                     IDIGMVSDVPGVTKKEYKIAPGTKNMTREAAMTRTEAIRAILTGIEIVGMLKSKSYEI
                     LATGEMGIGNTTTSSAVASVLTDIPVKLMTGRGAGLSADGLRRKIAAIERAISLHAPD
                     RGDPIDIISKVGGFDIAGLTGVFLGGAIFRIPIVIDGFISSVAALCAARLVPDCIGYM
                     LPSHCSGEPAASKVLDELGLSALLDCGMSLGEGSGAVAVMPLLEMGLSVYKSMSTFEE
                     IRVEQYEELK"
     gene            complement(5071..6414)
                     /gene="cbiT"
                     /locus_tag="ACZPD4_03210"
     CDS             complement(5071..6414)
                     /gene="cbiT"
                     /locus_tag="ACZPD4_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HIR1378117.1"
                     /translation="MCDKKRKVSLIGIGTGGVKEMTERAKEEILASDCLIGASRMLES
                     AACFLEQKKDIKRRDMLSEYRWDFIFSYVKEHEECSRTAVLFSGDIGFYSGAKKLEAA
                     LLHSEIDCTVELIPGISSVVYFAAKLGVSWDDAKIVSLHGNETLFIQTVDTNRKTFFL
                     LGGKDAGERLLNALLEYGMGELFLSVGRDLSYEDEKIIRKKAADFQIEEVRGLCTALV
                     ENENPAKRRGPHIRDDAFVRGKVPMTKEEVRAVSIARMELTEDAVVYDVGAGTGSVSV
                     EAALSGEQIKVYAIEKNPEAVLLLEKNRRKFRTDGIRIIEGSAPEALVHLEPPTHLFI
                     GGSSGNLKEILTVVKQKNPNVKIVISAISLETVRDVMDAEKEGLLTDMEVTQICASRS
                     RVLGQYHMMTGMNPVYIISAGGKEDGFGNFDHGDLQRKRENSGGVRTYGGISGSK"
     gene            complement(6407..7138)
                     /gene="cobJ"
                     /locus_tag="ACZPD4_03215"
     CDS             complement(6407..7138)
                     /gene="cobJ"
                     /locus_tag="ACZPD4_03215"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR1378118.1"
                     /translation="MNKIYVVGLGPGAGDQMTVQAKKVLDACPVIIGYTVYIDLVRPL
                     FPEKQFLSTPMRKEADRCKMAFEEVKKGVDVAMVCSGDSGIYGMAGLIYEIGRDYPEI
                     GIEIVPGITAASGGAAVLGAPLMHDFAVISLSDLLTPWELIEKRLCAAAEADFVICLY
                     NPSSRKRADYLEKACEIILKSRSEETVCGLVKNIGREGELYEIMTLKKLKTTQADMFT
                     TVYIGNSNTKELNGKMVTPRGYKDV"
     gene            complement(7135..7926)
                     /gene="cobM"
                     /locus_tag="ACZPD4_03220"
     CDS             complement(7135..7926)
                     /gene="cobM"
                     /locus_tag="ACZPD4_03220"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR1378119.1"
                     /translation="MVTFVGAGPGAKDLITVRGQRLLEEADIVIYAGSLVNPKLLYGC
                     KETCAVYNSAKMTLEEVLEVIKEGETQRKNIVRLHTGDPCLYGAIKEQMDELERLGIG
                     YEVCPGVSSFCAAAAALDAEYTLPGISQSVVITRMAGRTPVPEKESIRSFAAHGTTMV
                     IFLSTGMLKELSEELISGGYEQDTPAAIVYKASWPEEKVLNCTVKTLAQTAKEAGVTK
                     TALIVVGRVLDGKYERSKLYDPTFTTEFRKASSHVGKAEKENTKE"
     gene            complement(7966..8724)
                     /gene="cobI"
                     /locus_tag="ACZPD4_03225"
     CDS             complement(7966..8724)
                     /gene="cobI"
                     /locus_tag="ACZPD4_03225"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR1378120.1"
                     /translation="MKGKFTGVGVGPGDPQLLTLKALHAIQESDVVAIPVSDSGLKKP
                     GENADPKYMQQCTAYQIVRQVYSQIGEKEVIFLPMPMIKDREKLIEIHDEDAAFTAEK
                     LNEGKDVVFLTIGDPSIYSTCMYIHKRLKRMGYETELIPGIPSFCAAAARLDISLSEN
                     SDELHIIPASYGVEESMRLQGTKVFMKSGRKMAEVKRFLVENALEAKMVENCGMDSEK
                     LYFSTEEIPEQAGYYSLFIIKDESETQRKDRRKD"
     gene            complement(8732..9928)
                     /gene="cbiD"
                     /locus_tag="ACZPD4_03230"
     CDS             complement(8732..9928)
                     /gene="cbiD"
                     /locus_tag="ACZPD4_03230"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR1378121.1"
                     /translation="MKQEIGRNRSGLRLGYTTGSCAAAAAKAAVAMLLKEEEITHVKL
                     ITPKGIELYLEVESTIRTADYVQCAVEKYSGDDPDVTDGMKVYAKAEKIESFLKEEEA
                     EERCFEIKDHIVLAGGEGIGRVTKAGLEQQTGFPAINKIPRRMIADAVDEECRQYKYG
                     GKIKITLSIPGGEKIAKKTFNPRLGIVGGLSVLGTTGIVEPMSEKALTDTIWLEMKML
                     KETGYDRCYVVPGNYGMDFLREELGVDEEVTVKCSNYIGDTIDDAKLLGMKGILFIGH
                     IGKFVKLAAGVMNTHSRQADCRMEVLAVHAALEGADSHTVRSVMDCVNTSEALRVLKE
                     CGLLEKVMESVMERIEFYLTNRAGEALDIAAVVFSNEEGVLGKTSKADSLFEQTSRFV
                     RQKKGR"
     gene            complement(9953..10405)
                     /gene="bcp"
                     /locus_tag="ACZPD4_03235"
     CDS             complement(9953..10405)
                     /gene="bcp"
                     /locus_tag="ACZPD4_03235"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR1378122.1"
                     /translation="MLEAGMKAPEFALPDQNGKVHTLEEYKGKKVILYFYPRDNTPGC
                     TKQACGFGELYPEFSEKGAVVLGVSKDSVASHKKFEEKYGLPFTLLSDTELTCIQAYD
                     VWKEKNMYGKKTMGVVRTTYLIDEEGVIEKVFGKVKAAENPRQMLEEL"
     gene            complement(10450..11220)
                     /locus_tag="ACZPD4_03240"
     CDS             complement(10450..11220)
                     /locus_tag="ACZPD4_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR1378123.1"
                     /translation="MKKGILAVSFGTSHIDTMEKTIDVLEKEFAKTFEEAVVYRAFTS
                     NMIIKKLKRTANIKVQTVGEALEKMAADGIEEVIVQPTHVINGIENDRMLQEVMEHMH
                     LFRKVYVGKPLLSSVEDYKKAIHAVMEKTVIGEDEMLVLMGHGTDHHANSAYPTLEYT
                     FHSLGYQQVLVGTVESFPDLRNVMAKLEISGRKKVVLMPFMFVAGDHAKNDMAGEEDS
                     WKSELEEKGYEVRVILKGLGEFEGIRKIFAEHIEEAEM"
BASE COUNT         2791 a         3160 c         1832 g         3528 t
ORIGIN      
        1 catttacagc ccggatatgc cgctgactgc aaaagcgctc ttttcgccgc ctcgatcact
       61 tttttagacg gaccgagagg gttgatattt acggagaaat caatcatctc tccatatgta
      121 tatatatcgc ctccatgtgt atattgcata ttcagtctcc ttcttttgtc ttatctgacg
      181 aaaatccggc aaaagatcac atgatgagta cagtgcataa ttttattcct ccgaaaacaa
      241 acatcattaa caatgccgta acatacatta atctccctgc ttttataata tcttccgggt
      301 caatcggacg aagatcatct ccgataaact cctttttata aactttccca aaataaactg
      361 catctccggc gagccggatt ctaagcgctc cggcacagac cgattctgtc tgggcagcat
      421 taggacttgc atgttttcgt ctgtcacgca aatatatcct ccatgcattt ttcccgtcta
      481 atcccgcaag aaaacttgca cataccataa acatcgccgt cacacgggca ggaataaagt
      541 taaatacatc atccatcttc gccggtattt tcccgaaata caaatatttt tcattcttat
      601 acccgagcat agaatccatc gtatttaccg ctttatataa aaacccaaga ggcgctcctc
      661 caagcatcat ataaagaaga ggagctgtca ctccgtcaga tgtattttcc gctaccgtct
      721 caaccgcagc ctttgtcaca ccttcttcgg tcaggttttc cgtatctctc ccgacaatca
      781 tagaaaccgc tcttcgcgcc tccttcatat catttctctt taaacagtaa tacacttttt
      841 tgctttcttc actcagagat tttgccgcaa agatctgata gcaccaaaac gactctaaga
      901 aaaagcagat caacggatgg atattctgcg ccaccctcag tcccgcaaac ggaatcccaa
      961 acgaaaggat cgctacaata atccataaga cccctcctgc cgtcatattt ttcttatcat
     1021 tttcttttct cagtctcttt tccaaaaatg aaatcatatt tccgatcatt ctcaccggat
     1081 gatacagcca taccggatct ccaaacagac gatccaaaaa aaatccggtc acacaagcag
     1141 ccaacgacat catcttccgc tccttttctc cgcatttttc agcagttcaa ttccattctc
     1201 ccattccttc tccgatacag atacaagata accgcttcca ttctccacct gccagttata
     1261 aaaatctttt ttttcttcgt aaagagcgtg catcaccgcc atgatcgtac cgccatgaac
     1321 gacaaacgcc gcatatttca catgtcgggc aatcaaaaat ctgacctgtt ttttcattcc
     1381 ttccacacag cgctttcgga attcttcctg atcctctcct cccggaaacg ccgttatccc
     1441 tccgcttttc agccacttta tataagcggc gttctttttc agctgctcat atgtttttcc
     1501 ttcaaactgt ccgaaatcac actctctcat caaaggttcc aaaacgatag gacaccctga
     1561 taaatccgta ccccaaatcc gctttgccgt ttgtacacaa cgtttcatgg ggcttgcgat
     1621 aatatattcc ggtatgggat aactccgctt taaaaaagcg gtcttttccg acaacacttc
     1681 atccgtactt ccgatatact gtcttttttc atttcccggc gtagcaaaat gccggattaa
     1741 catgattttc aacatattcc tcccaaaaag gcgacaacag tcagcatgac gagttcacat
     1801 atctgtaaaa aatatcccgc caaatctccg gtaattccgc cgaactgttt aaaactcatt
     1861 ttataataat agaaaaatac aagcagcgcg gccgttacaa ttattgcacc ctgaacacga
     1921 aaaatataga ttgctcctgc tgccaaagac ataagccata tagataagac gatccgcgtc
     1981 ctcttcctat ccgccctctc ttgaaatgtt ctcaacaagc cgctgctttt cgctgatctg
     2041 aaacttacga ctgaaagtcc gctcagcgct ctcgatatcg gatacatcca tacggcaaga
     2101 gacagcgttt taagattcac ttcactccaa agagctgccg taataagaaa ataggcgcac
     2161 agaccgatca ctgcaaacgc tcctgtatga ggatctttca aaatctctaa ttttttctct
     2221 ctgtctttat aagaactgac ggcatcgatc gtatccgcaa acccatccaa atgaatcccc
     2281 cctgtcacag cgaccggtat catcgtcatt accgccgcaa aaaagagttt tccgcactct
     2341 gtatatcgca gaatcagctg acctgcgccg aataccagcg ctcctataat cacccctacg
     2401 accgggaaaa aacacatggc atacctcata tttttttcat cccaatctat attcggaaca
     2461 ggaagttttg aatacatagc caatgcaatt atcaaagatt tcagcatatt cattttcttt
     2521 ttgtctcctc atacatctta tccactctta caacacacct gcccttactt taattcttcg
     2581 tactgttcta ctcgtatttc ctcaaacgta ctcatgcttt tatatacgga caaccccatt
     2641 tccaaaagag gcattacggc gaccgctccg cttccttctc cgagagacat cccgcagtca
     2701 agcagcgccg atagtccaag ttcatccaat acttttgacg ccgccggttc gcccgaacaa
     2761 tgcgaaggaa gcatataacc gatacagtca ggaacaagac gagccgcaca aagcgccgcc
     2821 acagaagaga taaaaccgtc aatgacgatc gggatacgaa agattgctcc tccgagaaat
     2881 acgcccgtaa gacctgcaat gtcaaaaccg cccacttttg agattatatc gatgggatca
     2941 ccccgatcag gggcatgaag cgatattgct ctttcgattg cggcaatctt tcgtcttaat
     3001 ccgtctgcgc taagtcccgc tccccttcct gtcattaatt ttaccggaat atccgtcaat
     3061 accgatgcaa ccgcactgct cgtcgtcgta tttcctatcc ccatttctcc tgttgccaaa
     3121 atttcatatg acttcgactt caacattccc acaatctcaa ttcccgtaag gattgcccgg
     3181 attgcttccg tcctcgtcat ggctgcttcc cgggtcatat tttttgtacc aggtgcaatt
     3241 ttatattctt tctttgtaac accgggcaca tcagacacca tcccgatatc gatcggaaac
     3301 agatccactc ccgcgatttg agccatctta catacacttg tctcgctttt tgtaaaattt
     3361 tcggcgacaa cagccgtcac ttcctgcccg gtctgtgtaa cgccttcttc taccactccg
     3421 ttatccgcac acatgatgac aagacctttt ttgtccaaac tgtacgcagg acttcctttc
     3481 attcccgcaa tcttcgtgac ggcatcctcc aatttcccaa gactaaataa aggctttgcg
     3541 atctgtttcc atcgcttttt tgcctgcgcc gccgcctttt gatccggagg acagatctgt
     3601 tcaagatatt cgttcagccg ctttgtcact ttttctaatt ccggttctga ttgttctttc
     3661 atatttttcc tgtcttctga catatgattc cttctctgct tcttcttatt tgcttcgttt
     3721 tctttggcaa acagtctgca ctgatctgta aaacgctttg caaactgcgg acaggacggc
     3781 atatataaat gaggataccc cgcaaacaaa ctgccttctg tatgaacaca tttccaggac
     3841 gttcttcgat ccggcttcgc tgctgttaaa ccttctccgt tatccgaact gtcccaataa
     3901 tgaaactcat gccctttgat cacttctcct tgtttcagat aacagctttc cttttgttcc
     3961 ttttcatgac ttaactccac gtagccgaaa cgcacaagct ttcccgccgg ataagtcctg
     4021 ccgttcaaaa ctcctgccat ctcccataca atatgcctct catcttccat ctcctcatga
     4081 agatacataa aaccgccgca ctctgccaga cagggcattc cgcttctgat cgcatctttt
     4141 accgaccgac gcatagaatt gttctcggac agttccttac agttaagttc cgggtaacct
     4201 cccccgaaaa tcaaaccgga aatattttcg ggaatctttt gatctctcaa cggactgaaa
     4261 aacacaagtt ctgctccgct ttcttccaga agttctaaat tttctttata ataaaaacaa
     4321 aacgccgcat cctttgctac cccgacccga accttgccgt cgtctgagct tttatacttt
     4381 tccatatcgc attgaaacgg ctcccggctc tgcccgtttc gcatattctg cttgatctgc
     4441 cctttttccg ggctatttgc tttctgttcc ccttgtgcga tctgatacaa tctgtcaaga
     4501 tcaaccgtct ctgtcacaat atttcctgcc tttgtcatct gttctttcag atgacagatc
     4561 tcctgcggag taacaaggcc tagatgacgg ctttccagac aaaacgcttc atcctgcgga
     4621 atgtatccta atacgggaat atcataccct tctgatttca aatgatcttc cagcattttc
     4681 ttcaacctgg gatacaacat ttttgacacc cggtttaaaa tgatcccctg aatattgctg
     4741 tctttttgaa aggatacaat cccgctgaca acagcacaaa gggaaagcgc cgcccctttg
     4801 caaggaagaa tcaatactgc gggaatgtca agtgttcttg ctacatcata tgagcttgcc
     4861 gtaacagaat ccagtctcat cccatcataa tatcccatca cgccttctgt tataacaagt
     4921 tcagcatctg ccgcatgttt tgcgtattct tttctcagct catcttccga agaaaagaat
     4981 aaatccagat tttttgaatc tacgccaagc acttctttat gaaacatcgg atcgatatag
     5041 tcaggtccgc atttacagga tgatactttt ttattttgag cctgatatgc cgccataagt
     5101 gcgcacgccg ccgctgtttt cccgcttccg ctggagatcg ccgtgatcaa aattcccgaa
     5161 gccatcttcc tttcctcctg ctgaaataat atagacagga ttcattcctg tcatcatatg
     5221 atactgcccc agaacacgag aacgcgacgc acatatctgt gtaacttcca tatcggtcag
     5281 taacccttct ttttccgcgt ccatcacatc ccggactgtc tcaagcgata tagcgctgat
     5341 cacaatcttt acatttggat ttttctgttt cacaacagtc aatatttctt ttaaattacc
     5401 ggaacttcct ccgataaaca gatgagtcgg cggttccaaa tgcacgagcg cctccggcgc
     5461 gcttccttct atgatccgga ttccgtccgt cctgaatttt ctcctgtttt tttccaaaag
     5521 aagcaccgcc tccggattct tttcaatggc atacactttt atctgctcac ctgaaagagc
     5581 cgcctccaca gatacagagc cggttccggc gccgacgtca tacacaaccg catcttctgt
     5641 aagctccatg cgggcgatgc tgacggcgcg cacttcttct ttcgtcattg gaacttttcc
     5701 tctgacaaac gcatcgtcac ggatatgcgg accacgccgc tttgccggat tctcattttc
     5761 caccaatgca gtacataacc cccgcacttc ttctatttga aaatctgccg cttttttcct
     5821 tatgatcttt tcatcttcat aagaaagatc tcttccgacc gaaaggaaca gttctcccat
     5881 tccatactcc aaaagtgcgt tcagcagtcg ttctcccgca tcttttccgc caagaagaaa
     5941 gaacgttttt ctgttcgtat ctaccgtctg tataaacagc gtttcatttc catgaagact
     6001 tacgatcttt gcatcgtccc atgaaacacc gagttttgcg gcaaaataaa caacagatga
     6061 aatccccgga atcaattcaa ccgtacaatc gatctctgaa tgcaaaaggg ccgcttccag
     6121 tttttttgct ccgctgtaaa acccgatatc accggaaaac aaaaccgcag tccggctgca
     6181 ctcctcatgc tcttttacat aagaaaaaat aaagtcccac cgatattcac ttaacatatc
     6241 ccgccgtttt atatcttttt tctgctctaa aaaacatgcg gcgctttcaa gcatccgtga
     6301 agctccgatc agacaatcgc ttgcaaggat ctcttctttc gctctttcgg tcatttcttt
     6361 tactccgcct gtcccaatcc cgatcaaaga tacttttctt tttttatcac acatctttat
     6421 agcctctcgg agttaccatt ttcccgttta actctttcgt atttgagttt ccgatataaa
     6481 cagtcgtaaa catatctgcc tgtgttgttt tcagcttttt aagagtcatg atctcatata
     6541 actcgccttc tcttcctata tttttcacaa gaccgcacac cgtctcttcc gaccgtgatt
     6601 ttagaatgat ctcacaggct ttctcaagat aatccgctct cttcctgctg gacggattat
     6661 aaagacagat tacaaaatca gcctctgccg cggcgcaaag tctcttctcg atcagctccc
     6721 acggagtcag gagatcgctt aaactgatca ctgcaaaatc atgcatcaac ggcgcgccta
     6781 aaacggctgc tcctcctgat gccgccgtga tcccgggaac aatctcaatc ccgatctcag
     6841 ggtaatctct cccgatctca taaatcagac ctgccattcc ataaatcccg ctgtcaccgc
     6901 tgcataccat tgccacgtca acgccttttt ttacctcctc aaacgccatc ttacagcggt
     6961 cggcttcttt tcgcatcgga gtgctcaaga actgcttttc cggaaataaa ggacggacaa
     7021 gatcaatata taccgtatat ccgataatga ccggacatgc gtcaagaact ttttttgcct
     7081 gtactgtcat ctgatctcct gctcccgggc cgagtcctac aacatatatt ttattcattc
     7141 tttagtattc tccttttctg cctttccaac atgagaagat gctttccgaa attctgttgt
     7201 aaatgtcgga tcatataatt ttgaccgttc atactttcca tctaaaaccc tgcctactac
     7261 aataagcgcc gtcttcgtta cgcctgcctc ttttgcagtc tgcgccaatg tttttaccgt
     7321 acagttgagt actttttcct ccggccatga cgccttatac acgatcgcgg caggagtgtc
     7381 ctgttcatat cctccgctga tcagctcttc ggaaagttct tttagcatcc ctgtgcttaa
     7441 aaaaataacc attgttgtgc catgagccgc aaaacttctg atcgactcct tttcaggaac
     7501 cggcgttctt cctgccatac gggtaattac aaccgactgt gaaattcccg gaagtgtata
     7561 ttctgcatcg agcgcagctg ctgccgcgca aaaagagctg acgccggggc acacttcgta
     7621 tccgatccca agtctttcca actcatccat ctgctctttg atcgccccgt acaggcaagg
     7681 atcgcctgta tgaagccgga caatgttctt tctttgagtc tcaccttctt ttatcacttc
     7741 cagcacttct tccagtgtca tcttcgcact attgtaaact gcacacgttt ctttacagcc
     7801 atatagaagt ttcgggttca caagcgatcc tgcgtaaata acaatatccg cctcttctaa
     7861 aaggcgttgt ccacgcacag tgatcaaatc ttttgcaccc ggacctgctc caacaaatgt
     7921 gaccatcttt ctctttcctt tcgctttctt agcctcttta cttttttaat cttttctccg
     7981 atctttccgc tgagtttcgc tttcatcttt tatgataaac aacgaataat atcccgcctg
     8041 ttcggggatt tcttccgtcg aaaaataaag cttctccgaa tccataccac agttttccac
     8101 catttttgcc tctaaagcgt tctcgacaag aaaacgtttc acttccgcca ttttccgtcc
     8161 gcttttcata aatactttcg ttccctgcag cctcatactt tcttctactc cgtaggaagc
     8221 aggtatgata tgcagttcat cgctgttttc tgaaagggaa atgtcaaggc gagccgccgc
     8281 cgcgcaaaaa gaagggattc cgggaatcaa ctctgtttca tatcccatcc ttttcagtct
     8341 tttgtgtata tacatacagg tagaataaat gctcgggtct ccgatcgtaa gaaacacaac
     8401 atcctttcct tcatttaact tctctgcggt aaacgccgcg tcttcgtcat gaatctctat
     8461 cagtttctcc ctgtctttga tcatcggcat aggcagaaag atcacctctt tttccccgat
     8521 ctgcgaataa acttgtctga cgatctgata agccgtacac tgctgcatat attttggatc
     8581 tgcgttttca cccggctttt tcaaaccgct gtctgatacg ggaatcgcga caacatcact
     8641 ttcttgtatc gcatgcaatg cttttaatgt cagaagctgc ggatctcccg gcccaactcc
     8701 aacgcctgta aactttcctt tcatagcctc tttatcttcc tttcttttgc ctgacaaaac
     8761 gacttgtctg ctcaaacaga ctgtctgctt tcgaggtttt ccccaaaacc ccttcttcat
     8821 ttgaaaaaac aacagccgca atatccaacg cttcccctgc tctgtttgtc agataaaatt
     8881 ctattctctc cattacgctt tccattactt tttcgagaag tccgcattct ttgagtactc
     8941 ttaatgcctc cgatgtattc acacagtcca tgaccgaacg caccgtatgc gaatctgccc
     9001 cttctaaagc cgcatgtacc gccaacactt ccatcctgca gtctgcctgt cttgaatggg
     9061 tattcattac tcctgccgcc agtttgacaa attttccgat atgaccgata aatagaattc
     9121 ccttcatccc gagtaatttt gcatcgtcga tcgtatctcc gatatagtta ctgcatttta
     9181 ctgttacttc ttcgtccacg ccgagttctt cacgtaaaaa atccattccg taatttcccg
     9241 gaacaacata acacctgtca taccctgtct cttttaacat tttcatttcc agccatatcg
     9301 tatcagtcag cgctttttca ctcatcggct ctacgatgcc tgtcgttcca agaacagaca
     9361 ggcctcctac aatgccaagc ctcggattaa acgtcttctt tgcgatcttc tccccgcccg
     9421 gaatagacag cgtaatcttt attttcccac cgtacttgta ttgacggcac tcttcgtcta
     9481 ccgcatcggc aatcatcctg cgaggtattt tattgatcgc gggaaagcct gtctgctgct
     9541 ctaatcctgc ttttgtgacc cggccgattc cttctcctcc ggcaagaaca atatgatctt
     9601 tgatttcaaa acagcgttct tccgcttctt cctcttttaa aaatgactcg atcttctcag
     9661 cttttgcgta tactttcatc ccatctgtca catcaggatc atcgccgctg tatttttcaa
     9721 cagcgcactg cacataatcc gcagttctga tcgttgactc tacttccaaa tacagctcga
     9781 tccctttcgg agtgatcagt ttcacatgag taatctcttc ttctttaaga agcatcgcca
     9841 cagcagcttt tgccgctgcg gcagcacagc ttcctgtcgt atatccaagc cgaagtcccg
     9901 accggttcct cccgatctcc tgtttcattt tatgctattc tcctgatatg ctttacaatt
     9961 cttccagcat ctgccgtgga ttttccgccg cttttacttt accgaacact ttctcgatca
    10021 caccttcttc atcgatcaaa taagtagtgc ggacaacacc cattgttttc tttccgtaca
    10081 tattcttttc tttccataca tcataagcct gaatacatgt taactctgta tctgaaagaa
    10141 gtgtaaaggg caaaccgtat ttttcttcaa atttcttatg agatgcgacg ctatccttgc
    10201 ttactccaag cacaaccgct cctttttctg aaaattcagg atataattct ccgaaaccac
    10261 atgcctgttt tgtacatccc ggagtgttgt ctctcggata aaaatataag atcactttct
    10321 tccctttgta ttcttccaat gtgtgtacct ttccgttctg atccggcaat gcaaactccg
    10381 gcgctttcat tcctgcttct aacatttgta tttcctcctg ttttttattt tattcttttt
    10441 agcttgttct tacatttccg cttcttcgat atgttctgcg aatatttttc tgattccttc
    10501 aaattctccg agtcctttta aaataacccg gacttcatat cctttttcct ccagctcgct
    10561 tttccatgaa tcttcttctc ctgccatatc attttttgca tgatctccgg caacaaacat
    10621 aaacggcata agtacgactt tttttctgcc cgatatttcc agctttgcca ttacatttct
    10681 caaatcagga aagctttcca ctgttccgac aagaacctgc tgatatccaa gcgagtgaaa
    10741 cgtatactct aacgtaggat atgcggaatt tgcatggtga tctgttccgt gtcccatcag
    10801 aacgagcatt tcatcttcgc cgatcaccgt tttttccatc acggcatgaa ttgctttttt
    10861 ataatcttcc acagaactaa gaagcggttt ccctacatat acttttcgga acaggtgcat
    10921 atgttccatg acttcttgca gcatccggtc attttctatc ccgttgatca catgagtcgg
    10981 ctgaacgata acttcttcga tcccatctgc cgccattttt tctaaagctt cgcctactgt
    11041 ttgcaccttt atatttgcag tgcgctttaa tttttttata atcatattgc ttgtaaacgc
    11101 cctgtataca acagcttctt caaacgtttt tgcaaactcc ttttcgagca cgtcaatcgt
    11161 cttttccatc gtgtcaatat gacttgttcc gaaactgact gccagaattc cttttttcat
    11221 tttacatctc ctctcatttt tatttatttt attatttttt attttttgat attaattcag
    11281 atgtttagct tctttttatt atcatttttt a
//
LOCUS       DBMDXF010000047        11098 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1661_length_11098_cov_2.056379, whole genome shotgun sequence.
ACCESSION   DBMDXF010000047 DBMDXF010000000
VERSION     DBMDXF010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1661_length_11098_cov_2.056379"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="ACZPD4_03245"
     CDS             <1..574
                     /locus_tag="ACZPD4_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1378124.1"
                     /translation="DTPTPEEKFWTVTLDGNEVKVKDGEKLTKPADPTKEGYKFLGWF
                     VGDKEFNFDTPITEELNGIKIEARFEKIDTPTPGEKEWTVTLNGKEVRVKDGEKLTKP
                     ADPTKEGYKFLGWFVGDKKFNFDTPITKELNGIKIEARFEKIKDEPQQPNKGGAVQTG
                     DTTNIALWIACLGIAGAAVAVTLRSRRKRS"
     gene            872..943
                     /locus_tag="ACZPD4_03250"
     tRNA            872..943
                     /locus_tag="ACZPD4_03250"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:905..907,aa:Glu,seq:ttc)
     gene            1033..1104
                     /locus_tag="ACZPD4_03255"
     tRNA            1033..1104
                     /locus_tag="ACZPD4_03255"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1065..1067,aa:Cys,seq:gca)
     gene            1258..2163
                     /gene="nadA"
                     /locus_tag="ACZPD4_03260"
     CDS             1258..2163
                     /gene="nadA"
                     /locus_tag="ACZPD4_03260"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR1378125.1"
                     /translation="MTIREEIEQLKKEKNAVILAHYYVRPEVQEIADYIGDSFYLSKV
                     AVELEEQTIVFCGVSFMGESAKVLNPQKTVLMPDAKADCAMAHMADEETIRAMRKKYD
                     DLAVVCYINSTAELKRLSDVCVTSANAVRIVKALPNKNIFFIPDRNLAHFVAEQTPEK
                     NFVYNEGYCPIHETVKIEELKQAKAEHPEAEVLTHPECPKSLLDLSDYIGSTSGIIDR
                     VGKSDAEEFIICTENGVRYELEKRYPNKKFYFTKTEPVCEDMKMITLEKIAHVLRTGE
                     NEVQMDEALRAESKKPLEKMLDLAK"
     gene            2231..3481
                     /locus_tag="ACZPD4_03265"
                     /pseudo
     CDS             2231..3481
                     /locus_tag="ACZPD4_03265"
                     /inference="COORDINATES: protein motif:HMM:NF004820.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            3500..4366
                     /gene="nadC"
                     /locus_tag="ACZPD4_03270"
     CDS             3500..4366
                     /gene="nadC"
                     /locus_tag="ACZPD4_03270"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR1378126.1"
                     /translation="MNKITMKLQADHLIMEALKEDISSEDVSTNAVMKEAVPGEVDLI
                     CKEDGIIAGLDVFSRVFELLDENTKTELYCKDGDEVKSGQLMGKVKGDIRVLLSGERV
                     ALNYLQRMSGIATYTHSVAKLLEGTKTKLLDTRKTTPNMRVFEKYAVTTGGGYNHRYN
                     LSDGVLLKDNHIGAAGGVAQAVKMAKEYAPFVRKIEIEVETLDMVKEAVEAGADIIML
                     DNMTTEEMQEAIRIIDGRAETECSGNVTKENIARLTGLGVDYISSGALTHSSPILDIS
                     MKNLHPVKEDVR"
     gene            4363..4878
                     /locus_tag="ACZPD4_03275"
     CDS             4363..4878
                     /locus_tag="ACZPD4_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HIR1378127.1"
                     /translation="MTGSDRRKQIVDQIRNSTTPVAGKVLAAAYDVSRQVIVQDIALI
                     RAAGYDIISTNRGYIINEPAAVSRVFKVKHTDAQVEEELTEIIDLGGIVRNVMVNHRV
                     YGHMEAEINITSRRKVTEFMDDIQNGKSSPLKNITSDYHYHKVEADSEETLDLIEDML
                     RNKGFLVEDRG"
     gene            4913..6109
                     /locus_tag="ACZPD4_03280"
     CDS             4913..6109
                     /locus_tag="ACZPD4_03280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1378128.1"
                     /translation="MKEIFELLKQVLNREFIRAVLSNPRTKDGVVKAKVRPLEKNGDF
                     MFQVEKFTKTQAFHENINCKDAAEILAGHMENFRQMQIETVQAEYTVLVSKKGKISIK
                     RKNRKSAAPPAELSHDRKKRYILEEGTFVPFLNDLGVMTEEGKIVRTKTDKFRQINRF
                     LEFIEDILPQLDKGRELTILDFGCGKSYLTFAMYYYLKELKGFDIHVIGLDLKEDVIR
                     RCSGLAKKYGYEKLHFLVGDIADYEGVNEVDVVVTLHACDTATDYALAKAIGWNAKVI
                     LSVPCCQHEVNGQFAKADKTQCYDGCLDPIMDYGLLRERFAALVTDGLRAKYLEAAGY
                     DTQVLEFIDMEHTPKNILLRAVRADEKRVTKKSAGTAIEKCERFLQIEPTLGRLLADK
                     GENYKE"
     gene            6106..8559
                     /locus_tag="ACZPD4_03285"
     CDS             6106..8559
                     /locus_tag="ACZPD4_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378129.1"
                     /translation="MKKKIMKAGILIVVFCAAVIISSLVINKDVSDEAADMGAASLPE
                     ISFYVGETEVNPLFGYVNKMEIPAVRDTITPLKSDGSLTVKVEKYGNKIDKLKYEVYS
                     LDGTKKHAEGKADIVKETSQAELDLKGILSSEEPEAVLKVTLDTPLSQNGKTAGYYTR
                     IVRPKEVTTDQCLAFAKDFHTKALNKEATEELTAYLEPDEKSDNTTYQTVNIHSDITH
                     IQWGDMKPSVIGDVEWDIKESNTVYTSILAKYKVSCTDEEGAESIYNVKEFFRVRFLV
                     DTIYLLDYNRNMEQVFDGRESDFDENGIILGIIPKDISYEINKDQTSAAFVQAGELWL
                     YESKKGNLTKVFSMPDQEGRDTRGENDQHAVRVIGIDNKNNITFAVYGYMARGSHEGE
                     VGVGIYYYDAAENKIEEKAFITSTKSFAIAEDELGKMVYYNQSTSLLHVLADGTLYRI
                     DLKKDEKKVLAENLTDERYAVSDDGHLMVYQTGGKTDKSATLHIMNLKSGEDYTIKAE
                     DGENLRPLGFINGDFIYGKVNPADTGITVSGEEITPMYEVQIRNSKNKEAAQYNFTEQ
                     SIYTTDVLIDGNLLTFNRVIKDGETYNSTKQEYVTNNEERKESKIVFETYVSENTGKQ
                     MRFTFADGVKKKQKQNEKPIYQPGKKTLTIELKGKEKEEKYYVYGMGELAAVYNKAGY
                     AVQKAEQVSGVVISSEQKYVWEKGNRDLVYSTEAGKFQCEEGESSLDACERYMEQYHA
                     QRLDLTGCSLDQMLYVINRGCPMIAILESAHAVLLTGYTMTDITYVDPSTGESYTVGM
                     SEMENMTEAGGNTFIGYIR"
     assembly_gap    8633..8642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8784..9287)
                     /locus_tag="ACZPD4_03290"
     CDS             complement(8784..9287)
                     /locus_tag="ACZPD4_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1378130.1"
                     /translation="MKTTQQSKVLFMAQAAMIAAIYVVLTMLGASFAFGEVQIRFSEA
                     LTILPAITPAGIPGVFLGCLISNILGGAILPDIIFGSIATLIGALFTWQLRNKSKYLA
                     PVPPIIANALIVPFILKYGYAVPLPIPFMMLTVGLGEVISCGVLGMILYTALNKYKGV
                     LFKPSIS"
     gene            9590..9862
                     /locus_tag="ACZPD4_03295"
     CDS             9590..9862
                     /locus_tag="ACZPD4_03295"
                     /inference="COORDINATES: protein motif:HMM:NF037113.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HIR1378131.1"
                     /translation="MHTIGRINKSIYSCITEDIVTDEVIITDNQLQHILDRHPEVYKE
                     VTDYLNDIISAPDFIIKDKHKNTGLVVKRIKTKEEYAQMVLRICAS"
     gene            10367..10522
                     /locus_tag="ACZPD4_03300"
     CDS             10367..10522
                     /locus_tag="ACZPD4_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378132.1"
                     /translation="MSATTKEHAAVLDDVNWMMVASASALAAVLLILVSIACLLETPK
                     NNNSEGE"
BASE COUNT         3661 a         1808 c         2877 g         2742 t
ORIGIN      
        1 cgacacaccg acaccggaag agaaattttg gacagtgaca cttgacggaa acgaagtgaa
       61 agtaaaagac ggagaaaaac tgacgaaacc ggcagatccg acaaaagaag gatataaatt
      121 cctcggatgg tttgtaggag ataaggagtt taacttcgat acaccgatca cagaagaact
      181 gaacggtatt aagatcgaag ctcgttttga gaagatcgac acaccgacac cgggtgaaaa
      241 agaatggaca gtgacactta acggaaaaga agtcagagta aaagacggag aaaaactgac
      301 aaaaccggca gacccgacaa aagagggata caaattcctc ggatggtttg tgggagataa
      361 gaagtttaac ttcgacacac caatcacaaa agaactgaac ggtattaaga ttgaggctcg
      421 ttttgagaaa ataaaagatg agccacagca gccgaacaaa ggcggagcag ttcagacagg
      481 tgatacaaca aacatagcgc tgtggattgc atgtcttggt atagcaggcg ctgcagttgc
      541 tgtaactctt agatcacgca gaaaacgttc ttaatgaaat ggttgttttg attgacgaac
      601 ggtgatcagt tataaaaagt aaaatacaga tttttctgcc ggcaggattt atcctgccgg
      661 cagttttgca gtgtggatta aagataaaac aatagaatat ggaacgtaag aaatgttttc
      721 aaaaaagcta taaaaaatga gctttaaaaa atcgtaaaaa actgtaaaaa attttagtaa
      781 aaaaattgac agaataagaa aaaaagactt gcaattataa aagttatatg ataatattaa
      841 aaacggttct gctacagaac tagaaaacaa tggccccgtg gtcaagcggt taagacatcg
      901 ccctttcacg gcggtaacac gggttcgatt cccgtcgggg tcatttatca ttcggacaac
      961 agaagtattg ttgcgaaaag aatgataaaa ctatgcatat tattttacta tgtgtagtat
     1021 atatatgaat aaggcgcagt agccaagtgg taaggcgtgg gtctgcaaca ccctgattca
     1081 ccggttcaaa tccggtctgc gcctcttgag agatcccgaa agactaatgt ttttcgggat
     1141 tcttttttgc aaaaggcgag cggcacaact gtcttgtcat ttgtaaactc aagtgttata
     1201 atgagcatca cgacagaaac aaaggcgtat ggatttcatg cgggaaaggg tatacgaatg
     1261 acgattagag aagagatcga acagttgaag aaggaaaaaa acgcggtcat actggcgcat
     1321 tattatgtaa gaccggaagt tcaggaaatt gcagattata tcggcgattc tttttacctg
     1381 agcaaggtgg cagtagagtt ggaggagcag acgattgtgt tctgcggcgt ttcgtttatg
     1441 ggcgaaagtg cgaaagtgct gaatcctcag aagacggttc ttatgccgga tgcaaaagcg
     1501 gactgtgcga tggcgcatat ggcagatgag gagacgatcc gtgcgatgcg gaagaagtat
     1561 gatgatcttg cggttgtctg ctatattaat tcaacggcag aactgaaaag attatcggat
     1621 gtatgcgtaa cttcggcaaa tgcggttcgg attgtaaagg cacttcctaa caaaaacatt
     1681 ttctttattc cggatagaaa tctggcgcat tttgtggcgg agcagacgcc ggagaagaat
     1741 tttgtataca acgaaggata ttgtccgatt cacgagacgg taaaaataga ggaattaaag
     1801 caggcgaaag cggagcatcc ggaagcggaa gtgctgacgc atcctgaatg tccgaagtcg
     1861 cttcttgatc tttcggatta tatcggaagc acgtccggca ttattgatcg tgtgggaaag
     1921 agcgacgctg aggaatttat catctgcacg gaaaacggtg tgagatatga gcttgaaaaa
     1981 agatatccga ataaaaagtt ctattttaca aaaacagaac cggtctgtga agatatgaaa
     2041 atgatcactc tggaaaagat cgctcatgtt ttgagaacag gtgaaaatga agttcagatg
     2101 gacgaagctc ttcgtgccga atcaaagaaa ccgttggaaa agatgcttga tctggcaaaa
     2161 taacaggtga aaatgaaaga tataattaag gaatagtaag gtaagattag gggcgctaag
     2221 ggaagtggat atggaaaaaa taaaagaaaa acaagatcag gtcgatgtgg tcattgtcgg
     2281 aacaggagtc gcaggattat ttgcggcatt gaatctgccg cgtgatttta aaattatgat
     2341 gattaccaaa gcggatctgg agagcagcga ttcatttttg gcacagggcg gaatctgcgt
     2401 tttgcgcgat gaaagtgatt atgacagtta ttttgaagat acgatgcgtg cggggcatta
     2461 tgagaaccga aaagaatcgg tcgatattat gatccgcagt tcgcaggaga tcatcaggga
     2521 actgatcggg tacggcgttg aatttgaacg caggacagag catagtgatg atgtcatcgg
     2581 tgacagtgat atttctcgta catatgagta tacaagagaa ggggcgcatt caagtccgag
     2641 gattttattt catgaggata ttacaggaaa agaaattacg ggaaaacttt tggcgcgggt
     2701 gaaagagctt gacaatgtag agatttttga atatacgaca atgacggata ttatagagga
     2761 aggcggagta tgcagaggcg ttgtcatgca ggaacaagac ggaacgagcc gggcagttcg
     2821 ctcagcgtat acgatcgttg caagcggcgg aatcggagga ttgtaccgtc attccacaaa
     2881 ttttccgcat ttgacgggag atgctcttga aattgcgaaa aaacacggaa tcagactgga
     2941 acataccgat tatgttcaga ttcatccgac aacgctttat tctaagaagc cggggcggag
     3001 atttctgatt tcggaatcag tgcggggaga aggagctgtt ctgcttgata aagagggaaa
     3061 tcgttttgta aatgaattgc ttccgcggga tgtcgttaca aaagcgattc gcgaacagat
     3121 ggaaaaagac ggaacagatc atgtgtggct gtctatggag aatataggaa cagagtcgat
     3181 attgagtcat tttccgaata tatacagacg ttgtaaagaa gaagggtacg atgtgacgaa
     3241 agagccgatt ccggttgttc cggctcagca ttattttatg ggaggcatct gggtggacag
     3301 cgatagtcag acttctatgg aacggttatt tgcggcagga gagacgagct gcaacggagt
     3361 tcacggagca aatcgtctgg caagcaattc tttgctggaa agccttgtgt tcgcaaaaag
     3421 ggcggcgcag aagatcggaa gagaaaaaac ggataccgcc gcaaagcagg cgggagagaa
     3481 cgaaaaaagg agtggaaacg tgaataaaat tacaatgaaa ttacaggccg atcatttgat
     3541 tatggaggca ttaaaagaag atatttcaag tgaagatgtc agtacaaacg cagtgatgaa
     3601 agaagctgtt ccgggagagg ttgacctgat ctgtaaagag gacgggatta ttgcgggact
     3661 ggacgtattt tcaagggtgt ttgaactttt agatgaaaat acgaaaacag agctttactg
     3721 taaggacgga gatgaggtaa aatcaggaca gcttatggga aaggtgaagg gcgatatcag
     3781 agtgcttctt tccggcgagc gtgtggctct taattatctg caaagaatga gcgggatcgc
     3841 aacatataca cattctgtgg caaaacttct ggaaggtacg aaaacgaaac ttttagatac
     3901 gaggaaaacg acgccgaata tgcgtgtttt tgagaagtat gcagttacga caggcggcgg
     3961 atataatcac agatataatc tgtcggacgg agttttgctg aaggataatc acatcggagc
     4021 ggcaggagga gttgcacagg ctgtaaaaat ggcgaaggaa tatgcgccgt ttgtccgcaa
     4081 gattgagatt gaagtggaaa ctctcgacat ggtaaaagag gctgtagagg caggcgcaga
     4141 tattatcatg cttgataata tgacgacaga agaaatgcag gaagcaatca gaatcattga
     4201 cggaagagct gaaacggaat gttcgggaaa tgtaacgaaa gagaatattg cacgtctgac
     4261 aggtcttggc gttgattata tttcaagcgg agctttaaca cattcttctc cgatacttga
     4321 catttcgatg aaaaacctgc atcctgttaa ggaggatgtc agatgacagg ctctgacaga
     4381 agaaaacaga ttgtagatca gatcagaaac agcacgacgc ctgttgcggg gaaagttttg
     4441 gcggcggcat atgacgtaag ccgtcaggtg atcgtgcagg acatagcgct tatacgggca
     4501 gccggatatg atatcatctc tacgaacagg gggtatatta tcaatgagcc ggcagcagta
     4561 agccgtgtgt tcaaagtgaa gcatacagat gcgcaggttg aagaagagct gacagagatc
     4621 atcgatctgg gcgggatcgt gcgcaatgtg atggtcaacc acagagttta cgggcatatg
     4681 gaagctgaga tcaacattac atcccgcaga aaagtgacgg aatttatgga tgatattcag
     4741 aacggaaagt cgagtccgct gaaaaatatt acttcggatt atcattacca taaagtagaa
     4801 gctgacagtg aagagacatt ggatctgatt gaggatatgt tgagaaataa gggatttctt
     4861 gtggaggatc gcggttagat cattgaaagc gaatacgggc agaggttgga agatgaaaga
     4921 aatttttgag ttgctgaaac aagttttaaa cagagaattt atccgtgcgg tgctcagcaa
     4981 tccgaggacg aaggacggcg tcgtgaaagc gaaagtccgt cctcttgaga aaaacggcga
     5041 tttcatgttt caggtagaaa agtttacgaa gacgcaggcg tttcatgaaa acataaactg
     5101 caaagatgca gcggaaatac tggcaggtca tatggaaaat ttccggcaga tgcagatcga
     5161 gactgttcag gcggaatata cggttcttgt gagcaagaaa ggaaaaattt cgatcaagcg
     5221 gaaaaaccga aaaagcgcgg cgcctccggc agagctttca catgacagga agaaacgcta
     5281 tatattagaa gaaggaactt ttgttccgtt tttaaatgat ctcggagtta tgacagaaga
     5341 agggaagatc gttcggacga aaacggataa gtttcgccag atcaaccgtt ttcttgaatt
     5401 tatagaagat atccttccac agcttgacaa aggacgtgaa ttgacaattc tcgacttcgg
     5461 ctgtggaaaa tcatacctta cgtttgcgat gtattattat ttgaaagaac tgaaagggtt
     5521 tgacattcat gtgatcggac tcgatctgaa ggaagatgtg attcgccggt gtagcggact
     5581 tgcaaaaaaa tatgggtatg agaaactgca ttttctcgta ggagatattg cggattatga
     5641 aggagtgaac gaggtggatg tcgtcgtgac gcttcatgca tgtgatacgg cgacagatta
     5701 tgcgcttgca aaagcgatcg gttggaatgc caaagttata ttatcggttc cgtgctgtca
     5761 gcacgaagtg aacggacaat tcgcgaaagc ggataagacg cagtgttatg acggatgtct
     5821 ggatccgatc atggattacg gacttttgag ggagaggttt gcggcgcttg tgacggacgg
     5881 attgagagca aaatatttag aagctgcggg atatgatacg caggtacttg agtttatcga
     5941 tatggagcac actccgaaaa atattttgct tcgggcggtc agagcggatg agaaaagagt
     6001 tacgaaaaaa agcgcgggaa cagcgattga aaaatgtgaa cgttttttgc agatagagcc
     6061 gacgttgggg agattgttgg cagataaagg ggagaattac aaagaatgaa gaaaaaaata
     6121 atgaaagcag gtattttgat cgttgttttt tgtgccgcgg tcattatttc aagccttgtg
     6181 atcaacaaag atgtgtcgga tgaggccgcc gacatggggg ctgcatctct gcctgagatt
     6241 tctttttatg tcggagaaac agaagtgaat ccgctttttg gttatgtaaa caagatggag
     6301 atacctgctg tacgtgatac gatcactccg cttaaaagcg acggttcatt gactgtaaaa
     6361 gtagaaaaat acggaaataa gatcgataaa cttaaatatg aggtatattc gcttgacgga
     6421 acgaagaaac atgcagaagg caaagcggat atagtgaaag aaacttcgca ggcagaactg
     6481 gacttgaaag ggatcttgtc atcggaagag ccggaggcag ttttgaaagt gacgcttgat
     6541 acgccgctgt ctcaaaacgg aaagacggcg ggatattata cccgcattgt acgaccgaaa
     6601 gaagtcacga ctgatcagtg ccttgcattt gcaaaagatt ttcatacaaa agcgttgaat
     6661 aaagaagcga cagaggaact tacggcatat ttagagccgg atgaaaagag cgataatacg
     6721 acatatcaaa cggtaaatat tcattcagat attacgcata tacaatgggg agatatgaag
     6781 ccctccgtga tcggagatgt ggagtgggat ataaaagaaa gtaatacggt atatacttct
     6841 atactcgcaa aatataaagt ttcctgtacg gacgaagagg gagcggaaag catctataac
     6901 gtgaaagagt ttttccgggt gagatttctc gtagatacga tctatttgct cgactataac
     6961 cggaatatgg aacaagtatt tgacggaaga gaaagtgatt ttgatgaaaa cggaatcata
     7021 cttggcatta ttccgaagga tatttcctat gagatcaata aagaccagac ttccgccgcg
     7081 tttgtgcagg ccggtgaatt atggctgtat gagtcgaaaa aaggaaatct gacaaaagta
     7141 ttttccatgc cagatcagga aggcagagat acgcggggag agaatgatca gcatgcggtg
     7201 cgtgtgatcg gcattgataa taaaaataat attacgtttg ccgtttacgg gtatatggcg
     7261 cgcggcagcc acgaaggcga ggtcggcgtc ggcatttatt attatgatgc ggctgaaaat
     7321 aagattgagg aaaaggcatt tattacaagt acgaaatcgt ttgccatcgc agaagatgag
     7381 cttggtaaga tggtatatta taatcagagt acttcccttt tgcacgttct tgcagacgga
     7441 actttatatc ggatcgatct gaagaaagat gagaagaaag tgcttgcgga gaatcttact
     7501 gacgaacggt atgccgtttc agatgacggt catcttatgg tgtatcagac cggagggaaa
     7561 acagataagt ccgcgacgct tcatataatg aacttaaaga gcggagagga ttatacgatc
     7621 aaagcggaag atggggaaaa cctgcgccct cttgggttta tcaacggaga ttttatatat
     7681 ggaaaggtaa atcctgccga tacagggatc acggtatcag gcgaagagat cacaccgatg
     7741 tacgaagtgc agatcagaaa ctctaagaat aaagaagctg cgcagtataa tttcacagaa
     7801 caaagtattt atacgacgga tgtgctcatc gacgggaatc tgctgacttt taaccgcgtt
     7861 ataaaagacg gagaaacata taattccaca aagcaggaat atgttacgaa caatgaggaa
     7921 cgtaaagagt cgaagattgt ttttgaaaca tatgtatcgg aaaacacagg aaaacagatg
     7981 cgttttacat ttgcggacgg agtgaaaaag aaacaaaaac agaatgaaaa accgatatat
     8041 cagccgggca aaaaaacgct gacgattgag ctgaaaggga aagagaaaga agaaaaatat
     8101 tatgtttacg gcatgggaga actggcggct gtctataaca aagcagggta tgcggttcaa
     8161 aaggcagagc aggtttcggg agttgtaatt tcttccgagc aaaaatatgt atgggagaaa
     8221 ggaaaccggg atcttgttta ttcaacagaa gcaggtaagt ttcaatgtga agaaggagaa
     8281 agttctcttg atgcgtgtga gcgttatatg gaacagtatc atgcacagcg gcttgatctg
     8341 acaggctgtt ctctcgatca gatgttatat gtgatcaatc gcgggtgtcc gatgatcgcg
     8401 atattggaat cggcacatgc ggtgcttttg acaggatata cgatgacgga tatcacatat
     8461 gtagatccga gtacaggaga atcatataca gtcggcatga gcgaaatgga aaatatgaca
     8521 gaagcaggcg gaaacacatt tatcggatac atcagatgat aaaaggagaa ctttcctcag
     8581 gaagttgatt ggcgcggcac ataccggaaa ggtaaatata agtgataaaa aannnnnnnn
     8641 nngcgaaaaa cgtatctcac ggatattttt tgtatcgtat gttagcagcg cttcagatgt
     8701 ttggccgcaa taagcgcgga gtgcgttttg cgaatgagtt ttgcgaatga cgcaagctga
     8761 actgccgcat gctttagaat attttatgat atggaaggtt taaataaaac ccctttatat
     8821 ttattcagtg ctgtgtacag tatcattccg agaactccgc aggagatcac ttcccccaga
     8881 cctactgtca gcatcataaa cgggatcgga agaggaactg cataaccgta ttttaaaata
     8941 aacggaacga tgagcgcgtt tgcgatgatc ggaggaaccg gcgctaaata tttgctttta
     9001 ttgcgaagct gccatgtaaa taaagctccg atcaatgtgg cgatgcttcc gaaaataata
     9061 tccggcaaga ttgctccgcc taatatgttg ctgataagac atcctaaaaa tactccggga
     9121 attcccgcag gtgtgattgc gggcagaatt gtcagcgctt ccgaaaagcg gatctgcact
     9181 tctccgaatg caaaagatgc gccgagcata gtcagtacga cgtagattgc ggcgatcatc
     9241 gccgcctgtg ccatgaaaag cactttgctt tgttgagttg ttttcatttt tcaattctcc
     9301 tttagttttg ttttacgtgt ggaaggtcac gaacacacga ttcattttcc gcgaaacaat
     9361 gagccatgtg gaggtgcttg cacatccatt tggcgaatgt cgcgagagtc aaataccgca
     9421 ggtatttggc ttacgccgga aatcggcgaa acccttgtaa gaccttattt ttcgggtttt
     9481 tgtaacgtgg gttagtatag cacggagagc gttggaattc aagccgtccc cagcattctg
     9541 ttagttaatt ttccgatcgt cagcatctaa tgtaaaagag gtatcaaaag tgcataccat
     9601 tggtagaatt aataaaagca tatatagttg tataaccgaa gatatcgtaa cggatgaagt
     9661 tattattaca gataatcagc ttcagcatat tttggataga cacccagaag tatataaaga
     9721 agttacagat tatttgaatg atatcatatc tgccccggat tttattatta aagataaaca
     9781 taaaaataca gggcttgtgg ttaaaaggat aaaaacaaaa gaggaatatg ctcaaatggt
     9841 gttgcgaatt tgcgcatcgt aagataatgc tggatataaa aattcggtta tatcttgttg
     9901 ggaaataagt gaaaaacgtt tacagaatta cctcagaaat aaagaaatcc tttacaaaaa
     9961 ggaataaata ctatatactt tagatacaat aaataagaag ttgttcgagg tggtaaaatt
    10021 cgttgcaacc acgcacccat tgggtcaaaa gagatgcagg agaggcgacg cctgccgagt
    10081 aacttcttat tttgtaaata tcgacgattc accatcagcc agtttggttg gtggtatttt
    10141 tatattcaga aagtaggaaa tgccatgaaa gcagtatgcg ttaaaagtta ttatgacaag
    10201 tagctgaaga ggaaagtcac agttggagat gaactgaaac tgacagaaga gcggtttaaa
    10261 gagctgacct cggaaagtaa cggcgcaaag atagtgcttg taaaagcaag ggctgagaaa
    10321 agggcggttg taaagaaagg ataaggtgta gttaaaagca gatcttttgt cggcgacaac
    10381 gaaagaacat gcggcggttc ttgatgacgt aaactggatg atggtggcat cagcctccgc
    10441 attggcggct gtgctgttaa tcctggtcag tattgcttgt ttgctggaga caccgaaaaa
    10501 caataattca gagggtgagt aacatcgtcc cttttttaat tccataggta gggtggaaaa
    10561 aatttttgga aagcctcttg acaattacgc gtaacaataa tatattatgc gtaacgcgta
    10621 acatatggag gtgagaagaa tcgcggagaa aagtagagcc gattacatga aagccagacg
    10681 cgagaaacaa aaaacattta gtgttgctgt ggatagagaa aaaatgttga gatttgagaa
    10741 aaaaatatct gaacaaggaa aaacaaaatc cgaatggctt aatgaaaaaa tcgacgaaga
    10801 actaaaaaat aagaagtact cgtaattcca tcaagactac cgagtacttc aactaagaag
    10861 tttccttctg taaatactat aatgtagaat gagacttctt tcaagacgaa ttttaaaacg
    10921 agaattttta ttatgcagaa attaatgatt tttgaaggtc attaatttag gtcatactgt
    10981 ttccgaaagg agtcgatgaa aatggaaata ggttctgtaa ttcttttagg aggtattttt
    11041 ttagtcgtaa tttataggag cggtttatca aaagaattta aagagaataa tttagaat
//
LOCUS       DBMDXF010000048        10908 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1687_length_10908_cov_2.140094, whole genome shotgun sequence.
ACCESSION   DBMDXF010000048 DBMDXF010000000
VERSION     DBMDXF010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_10908_cov_2.140094"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="ACZPD4_03305"
     CDS             <1..245
                     /locus_tag="ACZPD4_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR1378133.1"
                     /translation="ETLLAKIDEMTQPQDYIRGDVDANSKVDVGDVRTALRYICKKTN
                     LTETQMKAGDVTGDEKVTIEDLRKILRYVCKKITEL"
     gene            complement(326..1717)
                     /locus_tag="ACZPD4_03310"
     CDS             complement(326..1717)
                     /locus_tag="ACZPD4_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1378134.1"
                     /translation="MINSLSELLYSHILIILLLGAGIFFTIKTKFVQIRMFIESLRVV
                     AQPGSDKNGLSSFQALMVSTASRVGTGNIAGISTAICLGGSGAVFWMWLTAILGSASA
                     FIESTLAQIYKHKAKDGSSYGGPAYYIQAALKKRWIGVLFAVVLILTYMGGFNLVASF
                     NIADSFRVYSFFNPEWTPLIVGAILAVVFALAVFGGSKKISQITEVLVPVMGIFYLAV
                     SLFIILKHYQLVPVMFTSIFNNAFDVQAIFGGFAGSCVMHGIKRGLFSNEAGVGSAPN
                     AAASAAVSHPVKQGLVQMLSVFIDTILICSATAFMLLCSGVEPTSKLAGMPWVQAAAS
                     SSLGGFGTIFITIALCLFAFTTLIGNFYYAEMGLKFLCGRKPPKAVINTFRIIAAAIV
                     LAGATMEFSIVWNTADVLMGLMALINLPVILILCKPAIQAMNDYTKQKKAGKDPVFKA
                     ADIQLKDKTDFWN"
     gene            2006..3472
                     /locus_tag="ACZPD4_03315"
     CDS             2006..3472
                     /locus_tag="ACZPD4_03315"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1378135.1"
                     /translation="MKLSKLLERLEYEVVQGSVETEVTTLANDSRKVESGSVFVCISG
                     AVFDGHQYVSDVAKKGAAAIIAERDIEVPKGVTVIKAENTRYALALMSAAYFGYPAEK
                     LKVIGITGTKGKTTTTYLIRSILEGVGHKVGLIGTIEAIIGDRKIPAANTTPESFTIQ
                     QYFAQMVEEGCDSVVMEVSSQGLMLHRTAGIPFEIGIFTNLGKDHIGPNEHKDFEDYK
                     RCKAMLFQQCKLGIANIDDPYYEDIFRGATCKVETFGFSPKADLRAENVNLVSKPGYL
                     GVSYHVAGKADFDVEIDIPGKFSVYNSLTAIAVCRHFGVPSEKITEALKTAHVKGRIE
                     MIKVSDDFTLMIDYAHNAMSLESLLTTLKEYEPKRLVCVFGCGGNRSKDRRYEMGEVS
                     GRLADLTIITSDNPRFEEPQEIINDIKVGIAKTDGAYVEICDRKEAIKYAIEHGQSGD
                     VIVLAGKGHEDYQEIKGVKYPMDERTLIAEVLEEIREN"
     gene            3493..5712
                     /gene="priA"
                     /locus_tag="ACZPD4_03320"
     CDS             3493..5712
                     /gene="priA"
                     /locus_tag="ACZPD4_03320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1378136.1"
                     /translation="MFADVIIDIQHEKLDKIFQYRIPERLEHEVEAGMAVVVPFGKGN
                     RKTKGYVTALSKTCDYDISKVKEIADISRSGVEIETKLIALAAWMKSNYGGTMLQSLK
                     TVLPIKEKENIKVKKRLRLLIDRETGEKQLHYYLEKNQKARARLMAALLDDPLLDYEL
                     VRKKLNITATVIRALEEQHVLAVESEQVYRNPVHAENIQKKEIIYTQEQKRAIMRFCK
                     DYENGIRKTYLLHGVTGSGKTEVYMEMIQSVVDKGKQAIVLIPEIALTYQTVMRFYRR
                     FKDRVSVMNSRLSAGERYDQMMRAKAGEVDVMIGPRSALFTPFPNLGLIVIDEEHEPT
                     YKSEQTPRYHARETALRRAQTEGASVVLGSATPSMEAMYRAKNGEYVLMEMKNRSKMQ
                     DPAEVYTVDMRKELKNGNRSILSEQLQRLMKERLEKKEQTMLFLNRRGYSGFVSCREC
                     GHVIKCPHCDVSLSVHRDGKMRCHYCGYEQTRVKICPECGSDHIGEFRAGTQQIEEIV
                     KTVFPQARVLRMDMDTTRQKDSHEKILSAFANEEADVLVGTQMIVKGHDFPNVTLVGI
                     LAADMSLYTDDYRSGERTFQLLTQAVGRAGRGDKKGEAVIQTYSPDHYAIATSAAQDY
                     EAFYEEEIRYRTMMGYPPAENLLAVFISSADEALLETGCRYLKEYMIKAKKDIEASVI
                     GPASPGIEKINDVYRKVIYVKTADYHDLVVLKDRTEKYIEINSGFDKMRIQFDFNPM"
     gene            5734..6234
                     /gene="def"
                     /locus_tag="ACZPD4_03325"
     CDS             5734..6234
                     /gene="def"
                     /locus_tag="ACZPD4_03325"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1378137.1"
                     /translation="MAIRVIREIGDDILEKQCKAVPKMTLRTKILIGDMLETMYESNG
                     VGLAAPQVGVLKRIVVIDVGEGPIVLINPQIIESSGEQTGEEGCLSVPGKFGIVTRPD
                     RVKVRAFDENMNEFEMEGEGLLARAFCHELDHLDGKLYVELAEDGLHEVSYDEEDQDD
                     AQGGEE"
     gene            6231..7223
                     /gene="fmt"
                     /locus_tag="ACZPD4_03330"
     CDS             6231..7223
                     /gene="fmt"
                     /locus_tag="ACZPD4_03330"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1378138.1"
                     /translation="MRIIFMGTPDFSVGTLEALVEAGHDVCLAVTQPDKPKGRGKEMQ
                     FTPVKEAAVKHGILVYQPKRVRDPECVEELRKYNADVMVVVAFGQILPKEILEMTPYG
                     CINVHASLLPKYRGAAPIQWAIIEGESVTGVTTMQMDEGLDTGDMILKTEIPIAEDET
                     GESLHDKLAEAGAALCVKTLHAIENKTAVFEKQPESPTAYAKMLTKDLGNIDWAKSAV
                     QIERLVRGLNSWPSAYTHRDGKVMKIWKALAKPQEECAEQTELGSEEHTAQKTGKNAM
                     PGTVVSVAKDSFCIQTGDGVLRILEVQMPGKKRMDTGSFLRGYKVEEGMVFGRE"
     gene            7223..8593
                     /gene="rsmB"
                     /locus_tag="ACZPD4_03335"
     CDS             7223..8593
                     /gene="rsmB"
                     /locus_tag="ACZPD4_03335"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR1378139.1"
                     /translation="MAKAINEREIVAAILMEVTENGRYSHIVLREVLTKYQYLEKRER
                     AFITRVTEGTLEHMTEIDYILDCFSKVKVKKMKPLIRAILRSAVYQIKYMDSIPDHAV
                     CSESVKLAVKKGFSGLRGYVNGVLRSVVKGIDEVEYPSDPIKRLSVRYSCPEWIIRLW
                     KETYDLSLIETMLKDFQTEKPTTIRRCRSRITMEQLKKRLEEEHVLVENHPYLKDALL
                     ISKYDYLESLETFREGLFVVQDISSMLVGELAHVQAGAQVIDLCAAPGGKALDVAERI
                     LMEGQAGCSGHVEARDLTEYKVSLIEENIERMGLCNVTAVCQDASVYDEKSAGKADIV
                     IADLPCSGLGVLGKKTDLKYKASPEGIVSLVKLQRKILSCAASYVKPGGELLYSTCTV
                     NPKENIENVHWFLKEFPEFYADDIREDLCLELRESVTENGCIQLLPGVHKSDGFFIAR
                     LRKKEV"
     gene            8664..9731
                     /gene="rlmN"
                     /locus_tag="ACZPD4_03340"
     CDS             8664..9731
                     /gene="rlmN"
                     /locus_tag="ACZPD4_03340"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1378140.1"
                     /translation="MKKESMRMKKDIRAYEYEELQKELERLGEKPFRAKQIYEWLHVK
                     LADRFDEMTNLSVKLREKLAEEYDIFPVQMAERQQSKLDGTNKFLFRLYDGNMVESVL
                     MRYKHGNSVCISSQAGCRMGCVFCASTIGGLKRNLSASEMLGQIYQIQKIIGERVSNV
                     VIMGTGEPLDNYNNFLKFIHILTDEHGLNISQRNVTVSTCGIVPKILELAEERLQITL
                     ALSLHGSTQEKRRKLMPVANKYELGEVLSACDTYFKKTGRRVTFEYSLVHEVNDTDED
                     AKELSELLAPRNCHLNLIPVNPVKERSFQRPSRKSALNFKNKLEKSGINVTIRREMGS
                     DIDGACGQLRRRYEEETMNEN"
     gene            9742..10485
                     /locus_tag="ACZPD4_03345"
     CDS             9742..10485
                     /locus_tag="ACZPD4_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1378141.1"
                     /translation="MRTFSITDVGMVRQVNQDYVYVTDRPLGILQNLFVVADGMGGHQ
                     AGDYASKYTVEVLNRELALSEGEDIERCLVGAIKTANREIIKEASRDEHLKGMGTTVV
                     AATISNQMMYFANVGDSRLYLINQGIQQLTKDHSLVEEMVRLGGIKPEEAKHHPDKNI
                     ITRAIGAKADVEVDFYEHRLKRGDIILMCTDGLSNMVEDEELFHIVQGGRDIVESGQA
                     LIEAAKENGGTDNIGVVLIEPFADEVSVL"
     gene            10482..>10908
                     /locus_tag="ACZPD4_03350"
     CDS             10482..>10908
                     /locus_tag="ACZPD4_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1378142.1"
                     /translation="MIKEGVCLGKRYEILGRIGSGGMADVYKGKDRKLNRYVAIKVLK
                     SDYRSDQVFIKKFLSEAQAAAGLMHPNVVNVYDVGQDRGLYYMVMELVEGITLKDYIK
                     KKGRLSAKETISISIQMVTGLQAAHNHHIIHRDIKPQNII"
BASE COUNT         3394 a         1962 c         2991 g         2561 t
ORIGIN      
        1 gtgaaacact tcttgcaaaa atcgatgaga tgactcagcc gcaggactat atcagaggcg
       61 atgtggatgc caacagtaaa gtcgatgtcg gagacgtccg cactgcgttg agatatatct
      121 gtaaaaagac caatttgacg gaaacacaga tgaaagccgg agatgtgaca ggagacgaaa
      181 aagtaacgat cgaagactta agaaagatct taagatatgt atgcaagaaa ataacagagt
      241 tataaaataa gaaagcaacg atataaataa aaagtgttca atattcaaaa accgggaaag
      301 tcagatcgac tgtcccggtt tctctttaat tccaaaaatc tgttttgtct tttaactgga
      361 tatcggcagc tttaaagact ggatcttttc cggctttttt ctgctttgta tagtcgttca
      421 tagcctgaat tgccggtttg cataagatga ggatgacagg aaggttgatt aaagccatca
      481 atcccatcag tacgtccgct gtgttccata cgatgctgaa ttccattgtc gcgcctgcaa
      541 gtacgatggc ggcggcgata atacgaaatg tattgatcac tgctttcggc ggttttctgc
      601 cgcagaggaa tttcagcccc atttccgcat agtagaagtt tccgatcagc gttgtaaagg
      661 caaacaggca aagtgcgatc gtaataaaga tcgtaccgaa accgccaagc gagctggaag
      721 ccgctgcctg aacccacggc attcctgcca atttagaagt cggctctacg ccggaacaaa
      781 gaagcatgaa cgctgttgcg gagcagatca ggatagtgtc gatgaataca gaaagcatct
      841 gcacaagtcc ttgttttacc ggatgggaaa ctgccgcgct tgccgctgca ttcggagcag
      901 aaccgacacc ggcttcgttg gagaacagtc ctcttttgat accgtgcata acgcaggatc
      961 cggcaaaccc tccgaagatg gcctgtacgt caaatgcatt attgaaaatg cttgtgaaca
     1021 ttacaggaac aagctgataa tgtttcagga tgatgaataa agaaactgca aggtaaaaga
     1081 tacccataac cggaacaagc acttccgtaa tctgtgagat ctttttactt cctccgaata
     1141 cagccaaagc aaatacaacc gcaaggatcg ctccgacaat gagcggagtc cattccggat
     1201 tgaagaaaga atagacacgg aaggaatccg caatattaaa tgaagctaca aggttgaatc
     1261 cgcccatata agtcaggatc aaaacaactg cgaaaaggac gccgatccaa cgcttcttca
     1321 aagcggcctg aatataatat gcaggtcctc cgtaagaact tccgtccttt gctttgtgtt
     1381 tgtaaatttg agcaagcgta ctttcgataa atgcagatgc gcttccgaga atagccgtaa
     1441 gccacatcca gaacactgcg cctgatccgc caagacagat cgctgttgag attccggcga
     1501 tgtttcctgt tccgactctg gaagcggttg ataccataag agcctgaaag gaagaaagtc
     1561 cgttcttatc gctgcccggc tgtgcgacaa cgcggagaga ttcgataaac atacggatct
     1621 gtacgaattt tgttttgatc gtaaagaaga ttcccgctcc gagaagcagt atgatcaaaa
     1681 tgtggctgta gagcagctcg ctcagcgagt tgatcatgtt tgtaataaaa tctgtgaaca
     1741 atagttttcc ctctctttcc taaagtcatg tttgctgtgt caatatcgct tatatgattg
     1801 acgatcgcgt actgcgaatg cagacagttt agatacattt cggtgtgaga acgacaaagc
     1861 gggactgatg tcaccgaaaa tgcatacata ccggttaatt atatagtaaa aacgtttgtt
     1921 ttgcaacaaa acgcttcttg tgttattcta taaaggtgtc aaaaggtcga ttttcaaaag
     1981 gaaaataaaa tcgggagggc tgagtatgaa attaagcaaa ttattagaac ggttagaata
     2041 tgaggttgtt caaggcagtg tcgaaacaga agtgacaacg cttgccaatg actcgagaaa
     2101 agtagaaagc ggatctgttt ttgtctgtat cagcggagcg gtttttgacg ggcatcaata
     2161 tgtttcggat gtggcaaaaa aaggcgcggc ggcgattata gcagaacggg atatcgaggt
     2221 gccgaaaggg gtgacggtga tcaaagcgga aaatacgaga tatgcgctgg cgctgatgtc
     2281 tgcggcgtat ttcggatatc cggcagagaa gctgaaagtg atcggaatta ccggaacgaa
     2341 aggaaagacg acgacaacat atctgatccg gtctatctta gaaggtgtcg gacataaagt
     2401 aggacttatc ggtacgatcg aagcgatcat cggagatcgg aagatccctg cggcaaatac
     2461 gacgccggaa tcgtttacga tccagcagta ctttgcacag atggttgaag aaggctgcga
     2521 cagtgttgtt atggaagttt cctctcaggg acttatgctt cacagaacgg cgggaattcc
     2581 gtttgagatc ggtattttta cgaatctcgg aaaagatcat atcggtccga atgaacataa
     2641 agactttgaa gattataaac ggtgcaaagc aatgctgttt caacagtgca aacttggcat
     2701 tgccaatata gacgatcctt attatgaaga tattttccgc ggcgcgacat gtaaagtgga
     2761 aactttcggt ttttctccga aagcggatct gagagcggag aatgtaaatc tcgtatcgaa
     2821 gccgggatat ttaggcgttt cgtatcatgt ggcggggaag gctgattttg atgtggagat
     2881 cgatatcccc ggaaaattca gcgtgtataa ttcgctgact gcgatcgcgg tgtgccgtca
     2941 tttcggtgtt ccgtctgaga aaataacaga agcgcttaaa acagcgcatg tcaaagggcg
     3001 tattgagatg ataaaagtat cggatgattt tacgctgatg atcgattatg cacataatgc
     3061 gatgagtctg gagagcctgc tgacaacact gaaagaatat gaaccgaaac gtcttgtatg
     3121 tgtgttcgga tgtggcggaa accgttcaaa agacagaaga tatgagatgg gcgaagtgtc
     3181 gggacgtctg gcggatctta cgatcattac ttcggataat ccgcgttttg aagaaccaca
     3241 ggagattatc aatgatatca aagtcggaat cgcaaagact gacggggcat atgtggagat
     3301 ctgcgacaga aaagaagcga tcaaatatgc catcgaacat ggtcagtcgg gagatgtgat
     3361 cgtattggcc ggaaaaggtc atgaagatta tcaggagatc aaaggagtca aatacccgat
     3421 ggatgaaaga acgctgatcg cagaggtact tgaggaaatc agggaaaatt aggaaagaca
     3481 aggggacaga gattgtttgc ggatgtaatc atagatattc aacatgaaaa actggataag
     3541 atctttcaat accggatccc cgagcggctg gaacatgaag tggaagcggg gatggcggtg
     3601 gtcgtacctt tcggaaaagg aaaccgtaag acaaaaggat atgtaaccgc attgtcaaag
     3661 acatgtgatt atgatatttc aaaagtgaaa gagatcgctg atatatcgcg aagcggcgtg
     3721 gaaatcgaga cgaagctgat cgcgctggcg gcatggatga agtcgaatta tggcggaacg
     3781 atgctgcagt ctttgaaaac ggttcttcct atcaaagaaa aagaaaatat aaaggtaaaa
     3841 aagcggctca gactgctgat cgaccgggag acgggggaaa aacagcttca ttattatctg
     3901 gaaaagaatc agaaagcgag ggcgcgtctg atggcggcgc ttcttgacga tccgctttta
     3961 gattatgaac ttgtcagaaa gaagcttaat attactgcca cggtgatacg cgcgttggaa
     4021 gaacagcatg ttctcgccgt agaatcagag caggtgtatc gtaatccggt tcatgcggaa
     4081 aatattcaaa agaaggagat tatttatacg caggagcaga aacgtgcgat tatgcgtttc
     4141 tgtaaggatt atgaaaacgg aatccgaaaa acatatttgc tccatggagt tacgggcagc
     4201 ggaaaaacag aagtttatat ggaaatgata cagtctgttg tagataaggg gaagcaggca
     4261 atcgttctga ttcccgagat cgcgttaact taccagactg tgatgagatt ttacagaaga
     4321 tttaaagacc gggtatctgt tatgaattcc cgactgtccg caggagaacg atacgatcag
     4381 atgatgaggg cgaaagccgg ggaagtggat gtgatgatcg gaccgagatc ggcgcttttt
     4441 actccttttc cgaatctggg actgatcgtg atcgatgaag aacatgagcc gacttataaa
     4501 agtgaacaga cgccgaggta tcacgcgcgg gaaacggcgc taagaagagc gcagacagaa
     4561 ggcgcgagcg ttgttctggg aagcgcgact ccgtcgatgg aagccatgta tcgggcaaaa
     4621 aacggcgagt atgttctgat ggaaatgaag aaccgttcta aaatgcagga tccggcagag
     4681 gtttatacgg tggatatgcg gaaagaattg aagaacggaa atcgttcgat actcagcgaa
     4741 caactgcaaa gactgatgaa agagcggctt gaaaagaaag aacagacgat gctttttttg
     4801 aaccggagag ggtattccgg gtttgtttcg tgccgggaat gcgggcatgt tataaagtgt
     4861 ccgcattgcg atgtgtcgct ttcggtccat cgggacggaa agatgagatg tcattactgc
     4921 ggatacgaac agacgcgtgt gaaaatatgt cctgaatgcg gctcggacca tatcggagaa
     4981 ttcagggcgg gaacacagca gatcgaagag attgtgaaga cggtatttcc gcaggcaaga
     5041 gtgctgcgga tggatatgga tacgacacga cagaaagatt cccatgagaa aatactgtct
     5101 gcatttgcaa atgaagaggc ggacgtactt gtcggtacgc agatgatcgt aaaaggtcat
     5161 gatttcccaa atgtaacatt agtcgggatt ctggctgcgg atatgtcgtt gtatacagat
     5221 gattacagat cgggcgagag aacgttccag cttttgactc aggcggtcgg aagagcgggg
     5281 cgcggagata aaaaaggaga agcggtgatt cagacataca gtcccgatca ttatgcgatc
     5341 gcaacgtctg cggcgcagga ttatgaggcg ttctatgagg aagagatccg atacagaacg
     5401 atgatggggt atcccccggc agaaaatctt ctggcagtgt ttatatcaag cgccgatgag
     5461 gcgttattgg aaacaggctg ccgttatttg aaagaatata tgataaaagc gaagaaggat
     5521 atcgaagcgt ccgtgatcgg cccggcaagt ccggggatcg aaaagatcaa cgatgtttat
     5581 cggaaagtga tctatgtaaa aaccgcggac tatcatgatc ttgtcgtgct gaaagaccgg
     5641 acggaaaaat atattgagat taattcggga tttgataaaa tgagaataca atttgatttt
     5701 aatccgatgt agcggataga aagaaggaga aaaatggcaa tcagagtaat tagagaaata
     5761 ggagacgata tattagaaaa acagtgtaaa gcagtgccga agatgacgct tcgcacgaaa
     5821 atactgatcg gagatatgct tgagacaatg tatgaaagca acggtgtcgg tcttgcggct
     5881 ccgcaggtcg gcgttttgaa aagaatcgta gtgatcgatg taggggaagg tccgattgtt
     5941 ttgatcaatc cgcagatcat cgaatcttca ggcgaacaga caggagaaga aggatgtcta
     6001 agcgtaccgg gaaaattcgg aatcgttaca agaccggacc gtgtaaaagt gcgggcgttt
     6061 gatgaaaata tgaacgagtt cgaaatggaa ggcgaaggct tgcttgcaag ggcgttttgc
     6121 catgaacttg atcatttgga cggaaaactg tatgttgaac tggcagaaga cggacttcat
     6181 gaagtgtctt atgatgaaga agatcaagac gatgcgcaag gaggggaaga atgagaatca
     6241 tatttatggg aacacccgat ttttcggtcg gaacactgga agcgcttgtg gaagcggggc
     6301 atgatgtgtg tctcgccgtc acacagcctg ataagccaaa aggcagagga aaagaaatgc
     6361 agtttacgcc tgtaaaggaa gcggcggtta agcatgggat tttggtgtat cagccgaaaa
     6421 gggtgcgcga tcctgagtgt gtggaagaat tgagaaaata caatgccgac gtgatggtcg
     6481 ttgttgcttt cggtcagatc cttccgaagg agattcttga gatgacgcct tacggatgta
     6541 ttaatgtcca tgcatctctt cttccgaaat atcgtggggc tgcaccgatc cagtgggcga
     6601 tcatagaagg agagagcgtg acgggcgtta caacgatgca gatggatgaa ggacttgata
     6661 caggagatat gattttaaag accgagatcc cgatcgcaga agacgagaca ggagagtcgc
     6721 ttcatgacaa actggcagaa gcaggcgccg ctctctgcgt aaaaacattg catgcaatag
     6781 aaaataaaac agcagtgttt gaaaagcagc cggaaagccc gactgcttat gcgaaaatgc
     6841 tcacgaaaga tcttggcaat atcgactggg cgaaatcggc agtgcagatc gagcgtctcg
     6901 tgcgtggatt gaattcatgg ccaagcgcat atacacaccg ggacggaaaa gtgatgaaga
     6961 tatggaaagc gttggcaaag ccacaggaag aatgtgcaga gcaaaccgaa ctgggaagcg
     7021 aggaacatac agcgcagaaa accgggaaga acgccatgcc gggaacggta gtgtccgtag
     7081 caaaagattc gttttgcata cagacgggag acggcgtgct tcgtatttta gaagtacaga
     7141 tgcccggcaa gaaacgaatg gatacaggct cgtttctgcg cggatataaa gtggaagaag
     7201 gaatggtgtt tgggagagaa taatggcaaa ggcgatcaat gaaagagaaa tcgtagccgc
     7261 aatccttatg gaagtaacgg aaaacggcag atacagtcat attgtattaa gagaagtttt
     7321 gacaaaatat caatatcttg agaaaaggga aagagcattt atcacccgcg ttacggaagg
     7381 aacgctggaa catatgacag agatcgatta tattctggat tgtttctcca aagtaaaagt
     7441 aaaaaagatg aagccgttaa ttcgggcaat tttaagaagc gcggtctatc agataaaata
     7501 tatggacagt attcccgatc atgcagtttg cagtgaatcg gtaaaactgg cagtgaagaa
     7561 agggttttcc ggtctgagag gctatgtcaa cggcgtgctg agaagtgttg tcaaaggaat
     7621 cgatgaggtg gagtatccgt cagatccgat caaacggctt tcggtacgat attcctgtcc
     7681 ggaatggatc atccgattat ggaaagaaac gtatgatctt tctttgatcg aaacgatgct
     7741 gaaagatttc cagacagaaa agccgacaac gatcagacgc tgcagaagcc ggattacgat
     7801 ggagcagctg aaaaagcggc ttgaggaaga acatgtttta gtggaaaatc atccgtattt
     7861 aaaggatgcg cttctcattt caaaatatga ttatttagag tctctggaaa catttcggga
     7921 aggtctgttc gtcgtgcagg atatcagttc gatgcttgta ggagaactgg cgcatgtgca
     7981 ggcgggagcg caggtgatcg atctgtgtgc ggcgccgggc ggaaaggcgc ttgatgtggc
     8041 ggaacgcatt ttgatggaag gtcaagccgg atgttccggt catgtggaag cccgtgatct
     8101 gacagaatat aaagtgtctt tgatcgagga aaatatagaa cgtatgggac tttgcaatgt
     8161 aaccgcggtt tgtcaggatg cgtctgtgta tgatgaaaaa tcagcgggta aagcggatat
     8221 tgtgatcgcc gatctgcctt gttccggtct gggagtgctt gggaagaaaa cagatctgaa
     8281 atataaagcg tcgccggaag gaatcgtaag tctcgtaaag cttcagcgaa aaatcctttc
     8341 ctgcgcggct tcttatgtga agccgggagg agaacttctg tacagtacgt gtactgtgaa
     8401 cccgaaagaa aatatcgaaa acgtacattg gtttttgaaa gaatttcctg aattttacgc
     8461 agacgatata agggaagatc tttgccttga attgagggaa agcgtgacag agaacggctg
     8521 tatccagctt cttccgggag ttcataaaag cgacggattt tttattgcgc gattgcggaa
     8581 aaaagaagtc tgaaaatgcc ggaacgccga aaaaggatga aatggcagga cgcgcaaaag
     8641 ggtgaaatgg caggatacga aaaatgaaaa aggaaagtat gagaatgaag aaagatattc
     8701 gggcatatga atatgaagaa ttacaaaaag agctggaacg tctcggagaa aaaccgtttc
     8761 gggcaaaaca gatatatgaa tggctgcatg tgaagcttgc agatcgtttc gacgagatga
     8821 cgaacctgtc tgtgaaactt cgggaaaagc ttgcggaaga atatgatatc tttccggtac
     8881 agatggcaga gcggcagcag tcgaaactgg acggaacgaa caagtttttg ttccgtctgt
     8941 atgacggaaa catggtggaa agcgttctta tgcggtataa gcacggtaat tcggtatgta
     9001 tttcctcgca ggcgggatgc cgcatgggat gtgtattctg cgcttcgacg atcgggggat
     9061 taaaacggaa tctgtctgca tccgaaatgc tcggtcagat ctaccagatc cagaaaatta
     9121 tcggagagag ggtttccaac gtcgttatca tgggaacagg agagccgctt gataattata
     9181 ataattttct gaaattcatt cacatactga cagatgaaca cggactgaat atcagtcagc
     9241 ggaatgtgac tgtttctaca tgcgggatcg taccgaaaat actggaactg gcagaggaac
     9301 gtctgcagat cacactggcg ctttctcttc acggatctac gcaggaaaag agaaggaaac
     9361 tgatgcctgt cgcaaataag tacgaattag gcgaggtact ttccgcctgc gatacttatt
     9421 ttaaaaagac aggacgcaga gtgacgtttg agtatagtct tgtacatgag gtgaacgata
     9481 cggacgagga tgcaaaggag ctttccgagc ttctcgcgcc gcgtaactgt catctgaacc
     9541 tgattccggt aaatccggta aaggagcgtt cttttcagcg tccaagccgc aaaagtgctt
     9601 tgaatttcaa aaataaactt gaaaaaagcg gaattaatgt tactataaga agagaaatgg
     9661 gctcggatat cgacggggcg tgcggtcagc ttagacggcg ctacgaggaa gagacaatga
     9721 atgagaatta aggagaagca tatgagaaca ttttcgataa cagatgtagg aatggtaaga
     9781 caagtaaatc aggattatgt ttatgtgact gacagacctc tcggaattct tcagaacctc
     9841 tttgtggtgg cagacggtat gggaggtcac caggcgggag attatgcttc taagtatacg
     9901 gttgaagtgc tcaacaggga attggcgcta agcgaaggcg aagatattga aagatgtctt
     9961 gtaggcgcta taaaaacggc aaacagggag atcataaaag aagcttcaag agacgaacat
    10021 ctcaaaggaa tgggaacgac ggttgttgcg gcgacgatct caaatcagat gatgtatttc
    10081 gcgaatgtag gagacagccg tttgtatttg atcaatcagg gcattcagca gcttacgaaa
    10141 gatcattccc tggtagaaga gatggtaagg cttggcggga taaaaccgga agaagcaaaa
    10201 catcatccgg ataaaaatat tattacgaga gcgatcggcg cgaaagcgga tgtagaggta
    10261 gacttttacg agcaccgtct gaaaagggga gacatcatat tgatgtgtac ggacggtttg
    10321 agcaatatgg tagaagatga ggaacttttt catattgttc agggaggaag agatatcgta
    10381 gagtccggtc aggctcttat agaagcggca aaagaaaacg gcggtacaga taatataggg
    10441 gttgtcctta tagaaccgtt tgcagatgag gtgagtgtat tatgattaaa gagggagttt
    10501 gcttaggaaa aagatatgag atactcggac ggatcggatc gggtggtatg gcggatgttt
    10561 acaagggaaa ggatcgtaaa ttaaaccgct atgtcgcgat caaagtatta aaaagcgatt
    10621 atcgttcgga tcaggtattt ataaagaaat ttttgagtga ggcgcaggct gcggcaggac
    10681 tgatgcatcc gaacgttgtc aatgtgtatg acgtcggtca ggacagaggt ctgtactata
    10741 tggtaatgga gcttgtcgaa gggatcactc tgaaagatta tataaagaaa aagggaagac
    10801 tttcggcaaa agagacgatc agtatttcca ttcagatggt gacagggctt caggcggcgc
    10861 acaatcatca tattatccac agagatatta agcctcagaa tattatca
//
LOCUS       DBMDXF010000049        10743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1720_length_10743_cov_2.158899, whole genome shotgun sequence.
ACCESSION   DBMDXF010000049 DBMDXF010000000
VERSION     DBMDXF010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_10743_cov_2.158899"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="ACZPD4_03355"
     CDS             <1..200
                     /locus_tag="ACZPD4_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378143.1"
                     /translation="DPVIGRLPNIILGIYCISQHKRHIGLSGTEPNFPVNTSLSPVFC
                     SRLFSEHCRTFSMNGPAIQLL"
     gene            155..595
                     /locus_tag="ACZPD4_03360"
     CDS             155..595
                     /locus_tag="ACZPD4_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039476.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HIR1378144.1"
                     /translation="MSHFFYEWSGDSTFIGLVCPISESAWEHMKLLFFPALLYCFLFK
                     LIYKNAVPNVSLPLLSGILTGTTLIPVFFYTYSGILGFTVVWIDIAIFYLCTGITFSM
                     ASKLYEQKNIARFRFLIYGLTILYSILFFIFTFIQPDIALFQNP"
     gene            complement(674..1177)
                     /locus_tag="ACZPD4_03365"
     CDS             complement(674..1177)
                     /locus_tag="ACZPD4_03365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1378145.1"
                     /translation="MTKLSTLCYIEKDDKYLMLHRTVKENDVNKDKWIGVGGHFEYGE
                     SPEECLLREVKEETGYTLTSWAYRGIVTFVYGEDVVEYMSLYTADGFEGEPIDCDEGE
                     LEWVEKSKIETLKLWEGDKIFFDLLERDCGFFSLKLVYNIEDILKSAVLDGKPLPLTE
                     DGKWIGK"
     gene            complement(1254..3098)
                     /locus_tag="ACZPD4_03370"
     CDS             complement(1254..3098)
                     /locus_tag="ACZPD4_03370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378146.1"
                     /translation="MLKKQTVNEDKIQKSKVQAEQSKPQSKDRKMSRGEILKKVFGHL
                     GKYRIFVVFSVLLAAVSVALTLYIPKLIGYAVDEIIGKGAVNFAGIFRILFQIGICTG
                     ITALAQWLMNVCNNKMTYQVVQDVRNEAFRKIQILPLKYIDNHPSGEIVSRVIADVDQ
                     FADGLLMGFTQLFTGAATIIGTLGFMLSVDVNITFVVVLITPISFFVANFIARKTFAM
                     FRLQSEIRGEQTGLIDEMIGNQKVVQAFGQEEKVTERFDKINEKLGSAYLRATFFSSV
                     TNPATRFINSLVYTGVGIAGAFAVVNGTLTVGQLTSFLSYANQYTKPFNEISGVVTEF
                     QNAVVCAGRIFELIEEIPQPEDAKDAAVLTHVRGHVLAEHVDFSYMPQQNLITDFNLK
                     VMPGQKIAVVGPTGCGKTTLINLLMRFYDVDGGCISVDGKDVREVTRESLRCAYGMVL
                     QETWLKTGTIRENILLGRSDASEEEMIEAAKASYIHNVILRLPNGYDTWVTEDGGGLS
                     QGQKQLLCIARVMLCRPPMLILDEATSSIDTRTEVKIQAAFTKLMEGRTTFIVAHRLS
                     TIREADVILVMKDGKVIEQGNHESLMAADGFYRHLYESQFLRGKDEDK"
     gene            complement(3091..4824)
                     /locus_tag="ACZPD4_03375"
     CDS             complement(3091..4824)
                     /locus_tag="ACZPD4_03375"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378147.1"
                     /translation="MRSLFIYLKNYKKETILAPLFKMLEASFELLVPLVMAAVIDKGI
                     AQKDSPYIIRMCLVLIVLGIVGLICSLTAQYFSAKAAAGFGTGLRHALFEHIQHFGFS
                     EMDEIGSSTLVTRMTSDVNQAQAGVNLVLRLFLRSPFIVFGAMAMSFMVDVKAAMVFV
                     VVIPLLSVVVFGIMLITMPLYKKVQSYLDEILLKTRENLIGVRVLRAFNKEEKEKAEF
                     EENNQMLTKEQKFVGSISGLMNPLTYIIVNVGIIVLIYVGAVRVNMGSLTQGEVVALV
                     NYMSQILVELVKLANLIITVTKAAACGKRIEAVLAIKPEMQSGSLLYTEEKRIPAVEF
                     SNVSLTYKNAGAPSLSNISFRAMPGETIGIIGGTGAGKSSIVNMIPRFYDASEGEVKV
                     FGHDVREYEIESLRSHIGVVPQKAVLFKGTVAENLRWGDENASEQTLEEALEISQAKE
                     FVDSKPGKLEFMIEQNGRNLSGGQRQRMTIARALVRKPDILILDDSASALDFATDAAL
                     RDAIKNMKENPTVFLVSQRAASVRYADRIVVLEEGKAVAVGTHEELMQNCSAYQEIYY
                     SQFPEEAVLHA"
     gene            complement(4826..5449)
                     /gene="nth"
                     /locus_tag="ACZPD4_03380"
     CDS             complement(4826..5449)
                     /gene="nth"
                     /locus_tag="ACZPD4_03380"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR1378148.1"
                     /translation="MKKRTEEILNLLDEQYGREYICYLNYETPWQLLIATMLSAQCTD
                     ARVNIVTKDLFQKYTSVDAFADADLKELEQDIKPTGFYRNKAKNIIACMKDIREKFGG
                     EVPRSLEDLTSLAGVGRKTANVIRGNIYHDASVVVDTHVKRISNRLGFTKQSDPEKIE
                     QDLMKELPKDHWILYNIQIITFGRSICTARNPKCGECFLKKYCTGRK"
     gene            complement(5468..5635)
                     /locus_tag="ACZPD4_03385"
     CDS             complement(5468..5635)
                     /locus_tag="ACZPD4_03385"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1378149.1"
                     /translation="MNVMPITELIDKVTEICKANGVKRLDLFGSFATGTATDTSDVDF
                     VVYRCKLTDYK"
     gene            complement(5858..7612)
                     /locus_tag="ACZPD4_03390"
     CDS             complement(5858..7612)
                     /locus_tag="ACZPD4_03390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1378150.1"
                     /translation="MSQKLENLLNLALDADQGERERSEELDVGYDKEENVWELIVKYS
                     GTLEAVRQTARSVTELLNGYAVIVIEEERIGQLAQLPEVEFIEKPKKLYFQTDVGRQV
                     SCIDIVQDMPLSLRGKGTLIGIVDSGIDYENAEFRNEDGTTRIVSLWDQSVNGRPPAG
                     YLAGTEYTREQIDAALATEDKEVRRQMVKTSDVSGHGTAVAGIAAGNGRGSEGRRFRG
                     AAPEAELIIVKMGAPREGGFPRTTELMRGVDYIVRKAVELRRPVAINISFGNTYGSHD
                     GTSLVERFLNDIADMWKNVICIGSGNEGASAGHVSGKVRRQISETVELAVQQREPALS
                     IQIWKSYVDEMGVSVISPSGRQAGPFYEFLGAQRYILGDTELLIYYGEPKPYSVKQEI
                     YLSLLPGKQYIESGVWKIVLTPGRIVDGEYQMWLPTQTSLNMGTAFLQPNSMSTLTIP
                     STASLAVTVAAYDARTFSYADFSGRGPAGMYEGENVLKPDIAAPGVRVTAPVPGGGYQ
                     SFSGTSFAAPFVTGSAALLMEWGIVRGNDPYLYGEKVKAYLRKGAKQLAGYERWPNAL
                     LGYGALCVRDSLPGLGSS"
     gene            complement(7816..9039)
                     /locus_tag="ACZPD4_03395"
     CDS             complement(7816..9039)
                     /locus_tag="ACZPD4_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1378151.1"
                     /translation="MKKNVIVGQSGGPTAVINASLYGVVNEALNRKDSFGAVFGMING
                     IEGFAEGRVMDMEELKRSGELELVKTTPGSYLGSCRYKLPKDLNDEVYPKLFERFKEY
                     DIGYFFYIGGNDSMDTVSKLSRYAEMVGSDICFLGIPKTIDNDLVNTDHTPGFGSAAK
                     YVASTVREIAVDASVYDNKKSVTIVEIMGRHAGWLTAASALARKFEGDNPVLIYLPEV
                     AFDTERFIQDVSASLEKVSNVIVCISEGINDGKGTFICELASEVGVDTFGHKMLTGSG
                     KYLENLIKDRLGVKVRSVELNVSQRCSSAMLSATDLQEAVDSGAYGVKCALHGESGKM
                     IAFVRADGEEYKLSYEAKDVNEICNREKGVPAGWITKDGSDVSDEFIRYAKPLIQGEV
                     QVPQQDGLPLFAYRK"
     gene            9250..10266
                     /locus_tag="ACZPD4_03400"
     CDS             9250..10266
                     /locus_tag="ACZPD4_03400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1378152.1"
                     /translation="MKYISFAIPCYNSEAYMEKAINSILIGGEDVEILIVNDGSKDGT
                     QEIAERYEAQYPTIVKAIQKENGGHGDAVNCGLSHATGKYFKVVDSDDWVDESSLFKI
                     LETVKTFVKEDTLVDMVISNYVYEKVGMEHKKVIRYDNVLPENTIFHWDEIGHFRLDQ
                     YILMHSVLYRTEMLKLCQLKLPKHTFYVDNIYVYYPLPHVRTLYYLNVDFYRYFIGRE
                     DQSVNEKIMIGRIDQQLFVTKTMISMYELRMISSKKLRKYMVNYLAIMMTVSSILCIR
                     SKKPENLTKKKELWSYLKRKDYKTYMKIRYGILGQTMNLPGRSGRKVSSLAYSVARRL
                     IGFN"
     assembly_gap    10400..10409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2858 a         2822 c         1785 g         3268 t
ORIGIN      
        1 cggatccggt cattggccgc ttgccgaata tcattttggg catctattgc atttctcaac
       61 ataaacgcca tattggattg tccggaacag agccaaactt tccggtaaat acatctttat
      121 ctccggtttt ctgttcacgt ctgttctcgg aacactgtcg cactttttct atgaatggtc
      181 cggcgattca acttttatag gtcttgtctg cccaatcagc gaatctgcat gggaacatat
      241 gaaacttttg ttctttccgg ctctgctcta ctgctttctt ttcaaactaa tatataaaaa
      301 cgccgttcca aacgtatctt taccactttt atccggcatt ctgacaggaa cgacacttat
      361 ccctgttttc ttttatactt actcaggcat actcggcttt acggttgtct ggatcgatat
      421 tgctattttt tacctttgta ctggaatcac tttttcaatg gcatccaaat tatatgagca
      481 gaaaaatatc gcacgtttcc gcttcctcat atatggactg actatattat attccatcct
      541 tttctttata tttaccttta ttcagccgga tattgccctg tttcaaaatc catgactttt
      601 cataccgcgt ccgcttacgc ctgcttcttt tttctatctg tctttcactt catttttcag
      661 tctgttttcc ggttcatttt ccaatccact ttccatcctc ggtaagagga agcggcttcc
      721 cgtcaagaac agcgcttttt aaaatatctt ctatattata aacaagtttc agagaaaaga
      781 agccgcagtc tctctccaac agatcaaaga aaatcttatc tccctcccat aacttcagcg
      841 tctcgatctt tgatttttca acccattcca actcgccctc atcacagtcg atcggttctc
      901 cttcaaaccc gtcagccgta taaagcgaca tgtattctac aacatcttca ccgtaaacaa
      961 atgtcacgat tccccggtaa gcccacgaag tcagcgtata tcccgtttct tctttcactt
     1021 ctcttagcaa gcactcttca ggactttctc catattcaaa atgtccgccg acaccgatcc
     1081 atttatcttt attcacatca ttttctttca ccgtacggtg aagcattaaa tatttatcgt
     1141 ctttttctat atagcataat gtacttaatt ttgtcatagc catcctcctt aatacggtct
     1201 ctatatttgt atctttatcc gttttcagac ttttttattt tcttcacttt ttatcattta
     1261 tcctcatctt ttcccctaag aaactgcgat tcatacaaat gtctgtaaaa tccgtctgca
     1321 gccatcaagc tctcgtgatt gccctgctca ataacttttc cgtctttcat aacgagaatc
     1381 acgtccgcct cgcggatcgt tgacagacga tgcgccacaa taaacgtcgt ccgcccttcc
     1441 atcaattttg taaaagccgc ctgtatcttt acttctgttc ttgtgtcgat cgatgacgtc
     1501 gcttcatcga ggatcagcat aggcggtctg cacagcataa cacgcgcgat gcataaaagc
     1561 tgtttctgtc cttgcgacag cccgcctccg tcttctgtca cccacgtgtc atatccgttt
     1621 ggcagacgca ggattacatt gtgaatataa gatgcttttg cagcttctat catttcctct
     1681 tctgacgcgt cggaccttcc taaaagaata ttttcccgga tcgttcccgt tttcagccac
     1741 gtttcctgaa gcaccatgcc atatgcacag cgcagacttt ccctcgtaac ctcacgcaca
     1801 tcctttccgt ctactgaaat acagccgccg tctacatcat aaaaccgcat aagcagattg
     1861 atcagcgttg tttttccgca tcccgtcggt cctacaacag cgatcttttg tcccggcata
     1921 actttcaaat taaaatccgt gatcagattc tgttgtggca tatacgaaaa atcaacatgc
     1981 tctgccagta catgtccccg gacatgagtc agaaccgccg catcttttgc atcttccggc
     2041 tgagggattt cttctattaa ctcaaaaata cgaccggcac atacaaccgc gttttggaac
     2101 tctgtcacaa ctccggatat ttcattaaac ggctttgtat actgatttgc ataactcaaa
     2161 aaacttgtaa gctgccctac tgtcagcgtt ccgtttacta ccgcaaacgc acctgcgatc
     2221 ccgactcccg tatatacgag actgtttata aaacgggtcg ccggatttgt aacggaagag
     2281 aaaaatgtcg ctctcaaata tgcgcttcct aatttttcat taatcttatc aaaccgctcc
     2341 gtcacttttt cttcctgccc aaacgcctgt actactttct gattgccgat catttcgtcg
     2401 atcagacctg tctgttctcc tctgatttca gactgaagac gaaacatcgc aaatgttttg
     2461 cgggcaataa agttcgccac aaaaaatgaa atcggagtga tcaaaacgac gacaaacgta
     2521 atatttacat caaccgacaa cataaatccc agcgtgccga taatcgttgc agctcctgta
     2581 aacaactggg taaatcccat caaaagtccg tccgcaaact gatctacatc cgcaataaca
     2641 cggcttacta tttctccgga tggatgattg tcgatgtatt tcagcggaag gatctgaatc
     2701 ttccgaaaag cttcgtttct cacatcctgc acgacctgat aagtcatttt attattgcac
     2761 acattcatca gccactgcgc aagcgccgta atacccgtac atattccgat ctgaaacaga
     2821 attcggaata tcccggcaaa gtttaccgct ccttttccta tgatctcatc taccgcataa
     2881 ccgatcagtt tcggaatgta gagcgtaagg gcgacgctga ctgctgccag caaaacagaa
     2941 aaaacaacaa aaatacgata cttcccaaga tgcccgaata ctttttttaa tatttctccc
     3001 cggctcattt tccgatcttt gctctgtggc ttactctgct ctgcctgcac ttttgacttc
     3061 tgtattttat cttcattgac tgtttgtttt ttaagcatgt aaaaccgcct cctccggaaa
     3121 ttgtgaataa taaatctcct gataagccga acagttttgc atcagctcct catgtgttcc
     3181 gaccgccaca gctttccctt cttccagcac aacgatccga tctgcatatc tgaccgatgc
     3241 cgcccgctga gagacaagaa atactgtcgg attttctttc atgtttttaa tcgcgtcacg
     3301 caaagccgcg tcggtcgcga aatcaagcgc cgacgcactg tcgtccaata taagaatatc
     3361 tggttttctt acaagcgctc ttgcgatcgt catacgctgt ctctgcccgc cggacaaatt
     3421 tctcccgttt tgttctatca taaattcaag ctttcccggc tttgaatcaa cgaactcttt
     3481 cgcctgtgaa atttcaagcg cttcttccag tgtttgttcc gatgcatttt catctcccca
     3541 ccgaagattc tccgcaaccg tccctttaaa aagcaccgct ttttgaggaa ccacgccgat
     3601 atgactgcgc aaactttcaa tctcatattc tcttacatca tgtccgaaaa ccttcacttc
     3661 tccttccgac gcgtcataaa accgagggat catgtttacg atcgatgatt ttccagcccc
     3721 tgttccgccg ataatcccta tcgtctctcc cggcattgcc cgaaacgaga tgttgctcaa
     3781 cgaaggcgct cccgcatttt tatatgtcaa tgacacattg gaaaattcca ccgcaggaat
     3841 ccttttttct tctgtataca acaaacttcc cgactgcatt tccggtttta ttgccaacac
     3901 agcttcgatc cgttttccgc atgcagccgc tttcgtcacc gtaatgatca gattcgccag
     3961 tttcacaagc tccacaagaa tctgcgacat atagttgaca agagccacta cttccccctg
     4021 agtcagactc cccatattta cacgaaccgc gccgacataa ataagaacta taatacccac
     4081 gttcacgata atatacgtca gtggattcat aagcccggag atacttccga caaatttttg
     4141 ttcctttgtc aacatttgat tgttttcttc aaattcagcc ttttcttttt cctctttatt
     4201 aaaagcacga agcactctta caccgataag attttctctc gtttttagaa gaatttcatc
     4261 taaatatgac tgcactttct tataaagagg cattgtaatc agcatgatcc caaatacaac
     4321 taccgacaaa agaggaatta caacaacaaa aaccattgcc gctttgacat ctaccataaa
     4381 agacattgcc attgcgccga atacaataaa aggcgaacgt aaaaaaagac gaaggacaag
     4441 gttcactcct gcctgcgcct gatttacatc acttgtcatt cgggtcacaa gcgtagaact
     4501 gccgatttca tccatttccg aaaaaccgaa atgttgaata tgttcaaaca gcgcatgccg
     4561 cagtcctgtt ccgaacccag ccgccgcttt tgccgaaaaa tattgagctg taagagaaca
     4621 tatcagcccg acgattccca atacaatcaa aacaagacac attcttataa tatacgggct
     4681 gtctttttga gcaattcctt tatcgatcac cgcagccata acaagaggaa caagaagttc
     4741 aaaagatgcc tccagcattt taaacagcgg agccaatata gtctctttct tataattttt
     4801 taaataaata aaaagtgatc tcatgtcatt ttctccctgt gcaatatttc tttaagaagc
     4861 attccccgca tttaggattt cttgctgtac agatacttct tccaaacgta atgatctgaa
     4921 tattatacaa tatccaatga tcctttggca gttccttcat cagatcctgt tctatctttt
     4981 cgggatcaga ttgttttgta aatcccagtc tgttagaaat ccttttcaca tgcgtatcca
     5041 caactacgct ggcatcatga tagatgttcc ctctgatcac attcgccgtc ttccgtccga
     5101 cgcctgctaa agaggtcaga tcttccagac tcctcggcac ttcaccgcca aacttctccc
     5161 tgatatcttt catacaggca atgatgttct tcgctttatt gcgataaaat cctgtcggct
     5221 tgatatcctg ctccaactcc tttaagtccg cgtcggcaaa tgcgtccaca gatgtatatt
     5281 tttgaaacaa atctttcgtc acaatattca ctctggcatc cgtacactga gcgctcaaca
     5341 tcgttgcgat cagcagctgc cacggcgtct cataattcag ataacaaata tattcccgtc
     5401 catattgttc atctaaaaga ttcaaaattt cttcggttcg ctttttcatg cagcttttat
     5461 cttctcctta tttataatcc gtcaacttgc atctgtatac aacaaaatca acgtcacttg
     5521 tatcggttgc agtccctgtt gcaaaagacc caaatagatc caaccttttt acaccatttg
     5581 ctttacaaat ttccgtaact ttatcaatca attccgttat cggcataaca ttcatcgtct
     5641 atcactcttc cttattcttc acttttgtta ttaacacact tggagtttta caattaatac
     5701 ttccttttat ttcatacaga tcagattcaa tgcaatattt cattcccatt gtatcagaac
     5761 tttttactct tttctattaa tgttccgcac ttcatcgtca tatttgcaat attttatata
     5821 tcatcagtta tcgaggacag cgcctgacta acgaaactta acttgagccc aatcccggca
     5881 ggctatcccg cacgcacagc gctccatacc caagcagcgc attcggccat cgctcgtatc
     5941 ccgcaagctg ctttgcgcct tttcttaaat aagcttttac tttttctcca taaagatatg
     6001 gatcgtttcc tctgacaatt ccccactcca tcaaaagcgc agcgctcccg gtcacaaaag
     6061 gagcagcaaa agaagttccg ctaaaagact gatatccgcc tcccgggaca ggcgctgtaa
     6121 cgcgcactcc aggtgcggca atatccggtt tcagcacgtt ctcaccttcg tacatccccg
     6181 ccggacctct tcccgaaaaa tccgcatacg aaaatgtccg ggcatcatat gccgccacgg
     6241 taacagcaag cgacgccgta gatggaatcg tcaatgtcga catactgttc ggctgtaaaa
     6301 acgctgtccc catatttaat gacgtctgtg tcggaagcca catttgatac tccccgtcca
     6361 caatacggcc gggagttaaa acaattttcc acacgccgct ttctatatac tgttttccgg
     6421 gaagaagcga cagataaatc tcctgtttca cgctgtacgg cttcggttct ccataataaa
     6481 tcaaaagttc tgtatctccg agtatatacc gctgagctcc caaaaattca taaaacggtc
     6541 ctgcctgcct tcccgacgga ctgatcaccg aaactcccat ctcgtcaaca tacgatttcc
     6601 atatttgaat actcagcgcc ggttctcttt gctgaacggc aagttctacc gtctcagata
     6661 tctgccgccg cacttttccc gacacatgac ctgccgacgc cccctcattt ccgcttccga
     6721 tgcagattac atttttccac atgtccgcaa tatcgttcaa aaaacgttct acaagagaag
     6781 ttccgtcatg agatccatat gtgttgccaa aactgatgtt tatcgccacc ggacgacgaa
     6841 gttctacagc cttacggaca atataatcta ctccccgcat cagttctgtt gttctcggaa
     6901 aaccgccttc tctgggggct cccatcttta cgatgatcag ttccgcctca ggcgcagctc
     6961 cccgaaacct tcttccctca cttccccggc cattccctgc cgcgattccc gccaccgctg
     7021 ttccatgacc actgacatcg gatgttttca ccatctgccg ccttacctct ttgtcctctg
     7081 ttgctaacgc cgcatcgatc tgctcccgtg tatactctgt tcctgccaaa tatcctgccg
     7141 gaggcctccc attaacagac tgatcccaaa gagacacaat ccgcgttgtt ccatcctcat
     7201 ttctgaactc tgcattttca taatcgatcc cactgtcaac aattccgata agagtccctt
     7261 ttcctctcaa agataacggc atatcctgta caatatctat gcacgacacc tgcctgccta
     7321 catcagtttg aaaatacagc tttttcggtt tttctataaa ttccacttcc gggagctgag
     7381 ccagttgtcc gatccgttct tcctcgatga caataaccgc atagccgttc aacagttccg
     7441 taaccgatcg cgccgtttgc ctgaccgctt caagtgttcc cgaatatttt acgatcagtt
     7501 cccacacatt ttcttcttta tcatatccaa catccagctc ttcggagcgt tctctttctc
     7561 cctgatcggc atccagagcc agatttaaaa gattttccag tttttgactc attcattctc
     7621 ctatgactgc agcacattca ttttttgcat tatatgtact tcaggaaact ttcaacataa
     7681 aaaatcatga tctttaccaa cctttttatc tccggcaaag accatgatct tcttttttct
     7741 ttattacgtc gtctatttat tttcttttct tgattttctt ttctttagtt ccttttcttt
     7801 agttcctctg tccgtttact tcctgtatgc aaacagcgga agtccgtcct gctgcggaac
     7861 ttgtacttct ccttgaataa gcggtttcgc ataacggata aattcatcac ttacgtctga
     7921 tccgtcttta gtgatccacc cggcaggcac acctttttca cgattgcaga tttcattcac
     7981 atcttttgcc tcgtaactaa gtttatattc ttcaccgtcc gctctcacaa aggcgatcat
     8041 ctttccgctt tcgccatgca aagcacattt tacgccatac gctcccgaat caaccgcctc
     8101 ctgcaaatcc gtcgcagaaa gcattgccga agaacatctc tgacttacgt tcaattcaac
     8161 agaacgaacc tttacgccga gacggtcttt tatcaggttt tccaaatatt ttcctgatcc
     8221 ggtcagcatt ttatgcccga aagtatccac tcctacctcg cttgcaagtt cacaaataaa
     8281 cgttcctttg ccatcgttaa ttccttccga gatacaaacg atcacatttg acactttttc
     8341 aagagacgcg ctcacatcct gtataaaccg ttctgtatca aacgccactt ccggcaaata
     8401 gatcagaacc ggattatctc cttcaaattt tcgtgccaat gcgcttgccg ctgtaagcca
     8461 tcccgcatga cgccccatga tctcgacgat tgtcactgat tttttattat catataccga
     8521 tgcgtcaact gcaatctccc gcacggttga agccacatat tttgccgcgc ttccaaaccc
     8581 cggcgtatga tctgtattga caaggtcgtt atcgatcgtt ttcggtatac ccagaaagca
     8641 aatatcgctt ccgaccatct ccgcataacg cgacagtttg ctcactgtat ccatcgaatc
     8701 atttcctccg atataaaaga aataaccgat gtcatattcc ttaaatcttt caaataattt
     8761 cggatatact tcgtcgttta gatctttcgg taatttatat ctgcaagagc caagatatga
     8821 accgggcgtt gtctttacta attccagttc tcccgagcgc ttcaattctt ccatatccat
     8881 gacccggcct tcggcaaacc cttcgattcc gttaatcatg ccgaacactg caccaaaaga
     8941 atcttttctg ttcaacgctt cattgacaac tccgtataag cttgcgttga taactgccgt
     9001 cggtccgccc gactgtccta cgatcacatt ttttttcatc tcttcctcct tattttaatc
     9061 accgaaagtt caaaaatcac attccgggat tgtaagcatc tgccaagatt tcggataatc
     9121 ccggccttta gcttttcgct attataaaaa cacttttact atatatgaaa aaattgaatt
     9181 ttacaatata gaataactaa tgtatactac accttagaca acatgacaga taaaatcagg
     9241 aggtatgata tgaaatacat ttcatttgca attccttgtt ataactcaga agcctatatg
     9301 gaaaaagcga tcaactcaat ccttatcgga ggagaagatg tcgaaattct tatcgtcaac
     9361 gatggttcaa aagacggaac gcaggaaatc gccgaacggt acgaagctca atatccgacg
     9421 atcgtcaagg cgatccaaaa agaaaacgga ggacacggcg atgcagtcaa ctgcggactt
     9481 tctcatgcca caggaaaata ttttaaagta gtagacagcg acgactgggt tgacgaaagc
     9541 tctctgttta aaattcttga aacagtaaaa acatttgtga aagaagacac acttgtcgat
     9601 atggtaatct caaactatgt ttacgaaaaa gtcggcatgg aacataaaaa agtgatccgc
     9661 tatgacaatg tgcttcccga aaatacgatt tttcactggg atgagatcgg acatttccgg
     9721 ctcgaccaat atattttgat gcactccgtt ctttatcgga cagagatgct gaaactgtgc
     9781 cagttaaaat taccgaagca cactttttat gttgataata tttacgtgta ttatccgctt
     9841 ccgcatgtac gcacccttta ttacctgaat gtcgatttct accgctattt tatcgggcgg
     9901 gaagatcagt ccgtcaacga aaaaatcatg atcggaagga tagatcagca attatttgta
     9961 accaagacaa tgatctccat gtatgaactg cggatgatct caagtaaaaa actgcgaaaa
    10021 tatatggtta attatcttgc gatcatgatg actgtttcat ccattttatg tatccgctct
    10081 aaaaaaccgg agaacttaac taaaaagaaa gaactgtgga gctacttaaa acgaaaagat
    10141 tacaaaactt atatgaagat ccgctacggt atactcggac agacgatgaa tctcccggga
    10201 agatccggac gtaaagtatc ttctcttgca tacagcgttg caagacgcct gatcggattt
    10261 aactaactta aaaaacggtg catcggaagc ttttccatgt accgttttct taattttcaa
    10321 ttttttgtct aacagttcag ccatacagat atccaagaag cacaaaacat gcttgcatgt
    10381 ttttgcaagt ggatatctgn nnnnnnnnnt ttttgtaaca gttcagccat acagtgtacg
    10441 ggaagcacaa aacatgtctg catgtttttg ctaacggaca tctgtatgcg ctaagcgccc
    10501 atttatgagt aaaacagtgg cgtcagccac aataagcacg tggtgcggtt ttgcgaatgg
    10561 cgcgtgctga attgttacaa ttttttcatt ttttcatttt ttttaaaaaa acacttgatt
    10621 tttttttaga gtctgatata ataaccatgt tgcttacggg aaacaacaaa aaacaacaca
    10681 aaacggtaca atgcggaatg gtgtaattgg cagcacagca gactctgact ctgttagtag
    10741 ggg
//
LOCUS       DBMDXF010000050        10698 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1726_length_10698_cov_1.891573, whole genome shotgun sequence.
ACCESSION   DBMDXF010000050 DBMDXF010000000
VERSION     DBMDXF010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_10698_cov_1.891573"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            181..660
                     /locus_tag="ACZPD4_03405"
     CDS             181..660
                     /locus_tag="ACZPD4_03405"
                     /inference="COORDINATES: protein motif:HMM:NF042632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6560 family protein"
                     /protein_id="HIR1378153.1"
                     /translation="MCAIELVINSLYNRKSITDNAGRNEFHYVLKIPGALKYTCMAFF
                     ILGLCMFCIFFVFKLQNNPTVTNGHLWMCLGVMAIGLLGIIRASKWKIAVNGNQMEIQ
                     RLFRKSIVLQISEIERVEIGKKNQLLLYASGKKLITIDFLTENYEYLEETLKRNGKY"
     gene            complement(728..1705)
                     /locus_tag="ACZPD4_03410"
     CDS             complement(728..1705)
                     /locus_tag="ACZPD4_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIR1378154.1"
                     /translation="MTKRKKGIIITSTCALLAGAAVIAELALGGGTGGNRFGRQQGWQ
                     NLQKEIFDSAYSEESYDSAVEVPEYEGEPYVILDGNEPAFTEDEMTTTVYEQYSPLDS
                     YGRCQTAEACVGEELMPDEKRGDISSVKPTGWQSVRYENVEGGSLYNRCHLIAYQLSG
                     ENANEENLITGTRYMNTEGMLPFENLVAEYVHETDNHVMYRVTPIFENENLVASGVQM
                     EAESVEDGGKGIKFNVYVYNIQPDIIIDYKTGDNRAEDDDEKEAAGTSGAGKVFVESE
                     YILNTSTHKFHRPDCAGVKDMKEKNKKPYKGKRSKLLEDGYSPCKSCNP"
     gene            1930..2505
                     /gene="lepB"
                     /locus_tag="ACZPD4_03415"
     CDS             1930..2505
                     /gene="lepB"
                     /locus_tag="ACZPD4_03415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1378155.1"
                     /translation="MANQCFDQKQESLEPEQEPSDKNELFHWILTLAIAVIIALCVNR
                     FVLVNAEIPSGSMENTIMTGDRVFGNRLAYIFGEPKRFDIIIFRYPDDEKQLFIKRII
                     GLPGETVEIHDGQIFIDGSKTPLKDVTTKEFMQGSFGPYTVPDNCYFVMGDNRNNSKD
                     SRYWEHTFVTDDEIVGKAFLRYWPLNKIKFL"
     gene            complement(2528..3217)
                     /locus_tag="ACZPD4_03420"
     CDS             complement(2528..3217)
                     /locus_tag="ACZPD4_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378156.1"
                     /translation="MNKKGCNLKKILIGVSVGVMILASVVGVYLYVNRFDASKYVQAV
                     LDVSYKNKTTLYQEVADVSEKEAKAVFEKNLDATMQGFDASDMPDSLKGKYRELFGEI
                     AKKVNYTVGEAQKEKDGTYIVSVKVKPITLFTDTYDLFLSRAEQYAKEVTDHVTKGAQ
                     MPTDEEMQEKIYQIYYEVLKEQAEVGMLYGRAEDVVLHIEKEGRGNYEIRQKDMKKLD
                     GLLIEEVHPIE"
     gene            complement(3324..5303)
                     /locus_tag="ACZPD4_03425"
     CDS             complement(3324..5303)
                     /locus_tag="ACZPD4_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6449 domain-containing protein"
                     /protein_id="HIR1378157.1"
                     /translation="MTSVKCFGSWLKEAGRGHLTALFGWTTFFIYTVLLVMRLSLDTS
                     YTFFGIGNTVLLWLSMGLGVLFSFLEFFYLLQQKKQDFYYSLPVKKGMIFWSRYTHGV
                     IHFFLPFLLMISVCGIWQAMVDPEFAPFVGSYTAKSILAFGFAFLIFYHIGILCVSVC
                     GNFICAVSLCVSIIAYSSLFIENVIKVFAKHYFVTYYKIELLEKLNNILSPFGLAKRL
                     AGLGIYEKRIALQYPVPAPYIIAAVVWAAVLFVLFSATQKKRKPEYVGTPFMFRNVQR
                     LTEILLSVLAGAWFAAFAAELTKTDSLLFGFVEALCGAAAAFFVQYLIELAVKKQGVS
                     LLRRKGQFAAVSIAAACIGPAMMFGARFYDGYFPKEAEAVSVVIEGVGMSRDEYEMLD
                     GQENTVTEKYLYRFRMTGEGKKMTLQWAKDLAVAVNGRDAEEQKSVTKATVCYETEGG
                     SRHYRTYPVTEESLRKFSSVYETKEYKTLAYEGVSLEEVGQDKFSWKDGVFSQTMKIE
                     AEEKEELLKRYKEDVFRFQMEQLTKACPCGILEISSPADGEETELIVYPFFEKTCDFL
                     ESRGIDPEKQIKDYPVESVEIKKTSAVLPNVSGGSYVHFYEEPEEVDQWKEKLIPKAF
                     DVQQILYPTDHTQNAEAVVTDEETASYVRVKCILK"
     gene            complement(5278..6174)
                     /locus_tag="ACZPD4_03430"
     CDS             complement(5278..6174)
                     /locus_tag="ACZPD4_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378158.1"
                     /translation="MICVKELVKNFDDVCAVNNITLNIPEGEMLGLLGTNGAGKTTLL
                     RMIAGVLEADGGSIVVDGANITSGDVSEEIFYLPDDPYFFPNASMEEMILFYKKYYPR
                     MDAEAAAYMAGKLNLETKRPLRTFSKGMKRQAFLILALCAGTKYLLCDEVFDGLDPIV
                     TEVMKNLFRQEMKERKFTVVVASHKLRELEDICHSIAILHKGGVLTSKDMRHQAGNIC
                     KYQCVFSEQTDLEEVKKAVDLIRFHADGYFVTLIVRMQPETMARLNSFSPVFMKEVPM
                     TLEEIFIAEMEGKEYDIRKVLR"
     gene            complement(6190..7173)
                     /locus_tag="ACZPD4_03435"
     CDS             complement(6190..7173)
                     /locus_tag="ACZPD4_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIR1378159.1"
                     /translation="MKIFVYNYREFDEAEYFEKFSKEYHVELGICKEAPSLENAYLVK
                     GYEYVSIITTKIDRPLLEKFREFGVKMISTRTVGYDHIDLEAARQLGIRVSNVSYSPE
                     CVADYTMMLILMSIRKMKRIMQRAEINDFSLPGIQGRELPNFTIGVLGTGRIGQAVIR
                     DLHGFGCKIYAYDKYENEAVKQFAEYADLSVIYEKCDLITLHMPLSEDNFHIIDASAM
                     KNMKDGVVIINTARGSLIDTKALIEALESGKVGAAGLDVIEDELGMYYYNRKSDILSK
                     RDLYVLRGFPNVIVTPHMAFYTDQAVSDMVKHSIESCILREAGKKDPWEVV"
     gene            7304..8089
                     /gene="pdaA"
                     /locus_tag="ACZPD4_03440"
     CDS             7304..8089
                     /gene="pdaA"
                     /locus_tag="ACZPD4_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HIR1378160.1"
                     /translation="MFSNISKKTFFLLPALFLSSYVIGAGAGSLTSAAAEGNWGLSFH
                     EENTPPTANASTDELAQYDAYYMQDTDEKILYLTFDAGYENGNTEKILDVLKKHHVSA
                     AFFVVGTYIESNPELVKRMCKDGHIVGNHTWHHPDMSQMSTLESFQKELTDVEKIYKE
                     TTGKDMVRFYRPPQGKYSKENLKMAQDLGYKTFFWSLAYVDWYDTDQPTKAEAFDKLL
                     KRIHPGAIVLLHSTSKTNADILDELLTKWKEMGYQIKPLTQLP"
     gene            complement(8098..8881)
                     /locus_tag="ACZPD4_03445"
                     /pseudo
     CDS             complement(8098..8881)
                     /locus_tag="ACZPD4_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
     gene            9237..9659
                     /locus_tag="ACZPD4_03450"
     CDS             9237..9659
                     /locus_tag="ACZPD4_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLC family EF-hand domain-containing protein"
                     /protein_id="HIR1378161.1"
                     /translation="MVYLVTGPRGSGKTQQMIDLANEKVKTSNGNVVFIKKSHKDTYT
                     VDFNIRVIRMADYPDVLTLEEFVGFLYGMAAGNHDIEAVFIDSVLKQANITLESLAAF
                     LHKLNKISTENNIDFYLSISADKKDIPGIDSSDCTVVS"
     gene            complement(9745..>10698)
                     /locus_tag="ACZPD4_03455"
     CDS             complement(9745..>10698)
                     /locus_tag="ACZPD4_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1378162.1"
                     /translation="LRFKYPTYIFNDLSFEVDEEGTPYWVCPVKKYNIGLFGGVTVDR
                     VVLCNAVTGETVDYKVKDVPQWVDRVYSADLLVQLYDYYGTLKHGYFNSVLGQKDCLA
                     TTDGYNYLAINDDVWVYTGVTSITGDQSNVGFVLMNQRTMETKFYEVEGATEASAMSS
                     AEGQVQNLKYVATFPLLLNISGEPTYFIALKDDAGLVKKYAMVNVQKYQIVAIGDTVS
                     ECESVYTDLMYENGIKEEEEDVREVQTITAPITKIAQGVVEGNSHYYIMVEGSDDIFD
                     VSVVDFIDIIRYNVGDQVTIEYKEGEKSNTVLSLTEVKTIQ"
BASE COUNT         3099 a         2678 c         1765 g         3156 t
ORIGIN      
        1 caaactattt attgacacag tgcttagatt caatgcaatg atattaaatc attcgttttc
       61 gataaatcaa atctaattta aagcaaagac aaacaataga taaaaaaaca gcgaacagcg
      121 cttagcaagg aggaacatgg gatgggagag ataataaaat ttcttatata tgctatttct
      181 atgtgcgcca tagagctcgt gatcaattct ttatataacc gaaaaagtat cactgacaat
      241 gccggtcgga atgaattcca ttatgtgctt aaaatccccg gagcattaaa atatacatgc
      301 atggctttct tcattttagg attatgcatg ttttgcatct tttttgtatt taaactacag
      361 aataatccaa ctgtaacaaa cggacaccta tggatgtgtt tgggggtaat ggcaatcggt
      421 cttcttggca taatccgggc aagcaaatgg aagatcgcgg taaacggaaa tcagatggag
      481 atacaacgtt tatttcggaa aagtatagtt ttacagataa gtgaaattga acgtgtggaa
      541 atcgggaaaa agaaccaact acttttatac gccagcggta aaaaactgat aacgattgat
      601 tttcttaccg agaattatga atacctggaa gagacattaa aaagaaacgg gaagtattga
      661 ccggctctgc gtatttcctc gcaagccgat ccaacttccc gccttaacat ttcaaaagtg
      721 taacctttca gggattacag cttttacagg ggctgtaccc atcctcaagc agcttagatc
      781 tttttccttt atatggtttc ttgtttttct ctttcatatc tttcaccccc gcacaatccg
      841 gcctgtggaa tttatgtgta ctcgtattta aaatatactc gctctctaca aacacttttc
      901 cggcgccgga tgtccccgcc gcctcttttt catcgtcatc ttctgctctg ttatctcctg
      961 ttttatagtc aatgataata tccggctgta tattgtacac ataaacatta aacttgattc
     1021 cctttccgcc atcttctaca gattccgctt ccatctgtac tcctgaagca acaagatttt
     1081 cattttcaaa tataggcgtt acacgataca tcacatgatt atctgtttca tgcacatatt
     1141 ctgccacaag attttcaaaa gggagcattc cctctgtatt catataccgt gttccggtaa
     1201 tcagattttc ttcatttgca ttttctccgg acaactgata cgcgatcaaa tggcagcgat
     1261 tatacagcga tcctccctcc acattttcat accgaacaga ctgccatccc gtcggcttta
     1321 cactgctgat atcaccgcgc ttttcatcag gcatcagttc ttctcccacg cacgcttctg
     1381 ccgtctgaca tctcccgtag ctgtccaaag gactgtactg ctcataaact gtcgtcgtca
     1441 tctcgtcctc tgtaaacgcc ggctcgtttc cgtccaatat cacgtatggt tctccttcat
     1501 attccggcac ttcaacagca gaatcataac tttcttcact atatgcagaa tcaaaaattt
     1561 ctttctgaag attctgccat ccctgctgcc tgccaaacct gttaccgcct gtaccgccgc
     1621 caagcgccag ttcggcaatg actgccgctc ctgctaacag cgcacatgta ctcgtaataa
     1681 taatcccttt ttttctcttt gtcatctttg atactcccat ttttcccgat cagatatatt
     1741 ttttgcaata cctgttttat ctgccggctg tttttcttcg tttatacgaa atcttattta
     1801 tgaaaaatca ttgtatcaca gaagattctt ttctgcaaaa tcagatcatg acaatactgc
     1861 atcttgcaaa tttttcatac actcgttatt attgaagtac ataaaattta cggaaagcag
     1921 gaatacacta tggcaaatca atgttttgac caaaaacaag aatcactcga accggaacaa
     1981 gaaccttccg acaaaaacga actcttccac tggatactga cactcgcaat cgcagttatc
     2041 attgcccttt gcgtcaaccg ttttgtcctt gtaaatgcgg aaattccttc cggttctatg
     2101 gagaatacga tcatgaccgg agaccgtgtt ttcggaaacc ggcttgctta tatattcggc
     2161 gaaccgaaac ggttcgatat cattatcttt cgatacccgg atgacgaaaa gcagcttttt
     2221 attaaacgca tcatcggact tcccggtgaa acagtggaaa tccatgacgg acagatcttc
     2281 atcgacggct cgaaaacccc tcttaaagat gtaacgacaa aagaatttat gcaaggttct
     2341 ttcggtcctt atactgtacc tgacaactgt tatttcgtca tgggggataa ccgaaacaac
     2401 tcaaaagact cccgctactg ggaacatact tttgttacgg atgatgaaat cgtcggaaaa
     2461 gctttcctac ggtactggcc tctcaacaaa atcaaattcc tctgatcaaa tcccgcttaa
     2521 gcagatctca ctcaatcgga tggacttctt caatcagaag tccatccagt tttttcatgt
     2581 ctttctgacg gatttcgtaa ttcccgcgcc cttctttttc tatatgcagt acaacatctt
     2641 ccgctcttcc ataaagcatt cccacctctg cctgttcttt tagcacctca taataaatct
     2701 gataaatctt ttcctgcatt tcttcatccg taggcatctg cgcgcctttc gtcacatgat
     2761 ctgtaacttc tttcgcatac tgctctgcac gactcaaaaa cagatcatat gtatcagtaa
     2821 aaagtgtgat cggttttact ttcaccgaaa cgatatatgt tccgtctttt tccttttgcg
     2881 cctcccctac tgtataattt actttttttg caatttcacc gaagagttct ctgtattttc
     2941 cttttaacga atccggcata tcagatgcat caaacccctg catcgtcgca tccagattct
     3001 tttcaaatac tgcttttgcc tccttttcag acacatctgc cacttcctga tacaaggttg
     3061 tcttattttt ataagacaca tcaagcacag cctgcacata ctttgatgca tcaaacctgt
     3121 tcacatacag ataaacgcca actacagagg ccagaatcat aacccccaca gaaacaccta
     3181 tcaatatttt ctttaagtta caaccctttt tattcatact tttccctcct gtcatttcgc
     3241 cacagtataa catcttccaa ataaaaaaaa agaagaactc tgtcatatag acaaaattct
     3301 tctttctatt atccatcact ttattatttc aatatacact tcactctcac ataagaagcc
     3361 gtctcctcat ctgtcactac cgcctctgcg ttttgggtgt gatctgtcgg atagagaatc
     3421 tgctgcacat caaatgcttt cgggatcagc ttttctttcc attgatctac ttcctcaggt
     3481 tcttcataaa aatgcacata acttccgccg cttacattcg gcaaaaccgc agaagttttc
     3541 tttatttcta cagattcaac aggataatct tttatctgtt tttccggatc aatcccccgg
     3601 ctttcaagga aatcgcatgt tttttcaaaa aacggatata cgataagctc ggtttcttct
     3661 ccgtctgccg gagacgagat ttcaagaatt ccgcatggac aggctttcgt aagctgctcc
     3721 atctgaaatc ggaacacgtc ttctttgtac cgctttaaaa gctcctcttt ttcctcagct
     3781 tctattttca tagtctgcga gaaaacgccg tctttccaac taaacttatc ttgtcccact
     3841 tcctcaagcg aaactccttc ataagcaagc gtcttatact cttttgtttc ataaacggag
     3901 gaaaacttgc gcagactttc ttctgtcacc ggataagtcc gataatgtct gctcccgcct
     3961 tctgtttcat aacaaacagt cgctttcgtc accgattttt gttcttccgc atctctgccg
     4021 ttcacagcaa ccgccaaatc ttttgcccat tgaagcgtca tttttttacc ttctcccgtc
     4081 atacggaagc gatataaata cttttctgtc accgtatttt cctgtccgtc gagcatttca
     4141 tattcatcgc ggctcattcc cactccctcg atcacaacgc ttaccgcttc cgcttctttc
     4201 ggaaaatatc cgtcataaaa acgtgcgcca aacatcatag cagggcctat acaggcggcg
     4261 gcaatactta ctgccgcaaa ctgccccttc ctccgcagaa gactaacccc ctgcttcttc
     4321 acagccagtt ctatcaggta ctgtacaaaa aaagctgccg ccgccccaca cagcgcttcc
     4381 acaaacccga aaagtaaact gtctgttttc gtcaattccg ctgcaaatgc cgcaaaccac
     4441 gctcctgcca aaacggaaag caatatttct gtcagtcttt gcacattgcg aaacataaac
     4501 ggtgttccta catattccgg ttttcttttc ttctgagtag cagaaaacaa aacgaacaga
     4561 acagccgccc atacaacagc agcgattatg taaggcgccg gaaccggata ctgcagagct
     4621 atcctcttct catatattcc aagtcccgcc agccgttttg ccaatccaaa cggagatagt
     4681 atattattta gtttttcaag caattcaatt ttgtaataag taacaaaata atgttttgca
     4741 aacactttga tgacattttc aataaataaa gaagaatacg caatgatcga aacacagagt
     4801 gagactgcac atataaaatt gccgcatacc gaaacacaca aaatccctat atgatagaag
     4861 attaaaaaag caaatccaaa cgcaagaata cttttcgcgg tataacttcc gacaaaagga
     4921 gcaaattccg gatcgaccat cgcctgccat attccacata cggaaatcat gagcaaaaac
     4981 ggaagaaaga aatggatcac cccatgcgta tatctagacc aaaagatcat tccttttttc
     5041 accggaagac tgtaataaaa atcctgtttc ttctgctgca gcaaataaaa gaactccaga
     5101 aaagaaaaca ggacgcccag tcccatactc agccaaagca gaactgtatt tccaatcccg
     5161 aaaaacgtat aggaagtatc aagacttaaa cgcattacaa gaaggacagt atatataaaa
     5221 aatgttgtcc atccaaacaa cgcggtaagg tgtcctcttc cggcttcttt cagccaacta
     5281 ccgaagcact ttacggatgt catactcttt tccctccatt tcggcaataa aaatctcttc
     5341 taacgtcatc ggcacttctt tcataaacac aggcgaaaaa ctgttcagcc gggccattgt
     5401 ttccggctgc atacgaacga taagcgtcac aaaatatcca tccgcatgaa aacgaataag
     5461 atcgaccgcc ttttttactt cttcaagatc ggtctgttcc gaaaatacac actgatattt
     5521 gcatatattt ccggcctgat gcctcatatc tttcgaggtc aggactcctc ctttatgaag
     5581 gatcgcgata ctgtggcata tatcctcaag ttcccgaagt ttatgagatg ccactaccac
     5641 cgtaaacttc cgttctttca tttcctgacg aaacaggttt ttcatcactt ccgtcacgat
     5701 cggatcaaga ccgtcgaaca cttcatcaca gagaaggtat tttgttccgg cacaaagggc
     5761 aaggataaga aatgcctgac gtttcatacc ttttgaaaat gtacgaagtg ggcgcttcgt
     5821 ttccagattc aatttccccg ccatataagc cgccgcctca gcatccatgc gaggataata
     5881 tttcttataa aacaaaatca tttcttccat agaggcgttc ggaaaaaaat aaggatcatc
     5941 cggcaaataa aaaatctctt cagatacgtc tccggatgtg atatttgcac cgtcaacgac
     6001 aatgctgccg ccatcggctt ccagcactcc cgcaatcata cggaggagcg ttgtcttccc
     6061 cgctccgttc gttccgagca ggccgagcat ctctccttcc ggaatattta aagtaatatt
     6121 attgaccgca catacatcat caaaattttt cacaagttct ttcacgcaga tcatctgaat
     6181 ccccttttct taaacaacct cccacgggtc tttctttccg gcttcacgga gaatacagct
     6241 ttctatcgaa tgttttacca tatcgctcac agcctgatcg gtataaaacg ccatatgcgg
     6301 cgtgacgatc acattcggga aaccacgcag aacatacaga tctcttttgg acaatatatc
     6361 tgatttcctg ttgtaataat acattccaag ttcatcttcg atcacatcca gaccggctgc
     6421 gccgactttc ccggattcca aagcttcgat caatgccttt gtgtcgatca gacttcccct
     6481 cgctgtattg ataataacga caccgtcttt catatttttc atcgcagacg catcgataat
     6541 atggaaatta tcttccgaaa gcggcatatg caaagtaatc agatcacact tttcataaat
     6601 caccgaaaga tccgcatact ccgcgaactg ttttaccgct tcattctcat atttatcgta
     6661 cgcataaatc ttacacccga aaccgtgcag atcccggatc accgcttgtc cgatcctccc
     6721 cgtaccgaga actcctattg taaaattagg aagctcacgt ccttgaatcc ccggcaatga
     6781 aaaatcattg atctccgccc tctgcatgat ccgtttcatt tttcggatcg acatcaaaat
     6841 aagcatcatc gtataatccg ccacacattc cggcgaatag gatacgttgc tcacgcgtat
     6901 tccgagctgc cgcgccgcct ccaggtcaat gtgatcgtat ccgactgtcc ttgtcgatat
     6961 catcttcacc ccgaactcac ggaacttttc caaaagaggt cggtcgatct tcgttgtgat
     7021 aatgcttaca tattcatacc cttttacaag atatgcattt tcaagcgatg gcgcctcttt
     7081 gcagatacca agctccacat gatactcttt tgaaaatttt tcaaaatatt ccgcctcgtc
     7141 aaactctctg taattatata caaaaatttt cattctcatc acctcgcaca ataatatttg
     7201 ccttatttta ctcccctttt ctcgaaatgt ctacctctgt tttgttcata cttttccctg
     7261 ccttttcata taaatatgac aaaacttaag aggtttatct actatgtttt caaacatatc
     7321 taaaaaaact tttttcttac ttcccgctct ttttctctca tcctatgtga tcggagccgg
     7381 agccggaagc ctcacctctg ccgctgccga aggaaactgg ggattaagtt ttcatgaaga
     7441 aaacacaccg ccgacagcta atgcttccac agatgaactt gcacagtatg acgcttatta
     7501 tatgcaagat acagatgaaa aaatactcta tcttacattt gatgcaggat acgaaaacgg
     7561 aaacacagaa aaaattttag acgtattaaa aaagcatcac gtatccgctg cttttttcgt
     7621 tgtcggaaca tatatcgagt caaatcccga actcgtcaag aggatgtgca aggacggaca
     7681 catcgtagga aaccatacat ggcatcatcc tgatatgtcg caaatgtcaa ctcttgaatc
     7741 atttcaaaaa gaattaacgg atgtagagaa aatatataaa gaaaccaccg gaaaagatat
     7801 ggttcgtttc tatcgtcctc cgcaaggaaa atacagcaaa gaaaacttaa aaatggctca
     7861 agatctcgga tacaaaactt ttttctggag tcttgcctat gtcgactggt acgatacaga
     7921 tcagcctaca aaagcagaag cgtttgataa actgctcaaa cgcatccacc caggcgcgat
     7981 cgtcctgctc cacagcacat caaaaacaaa cgccgacatc ttagatgaat tactgacaaa
     8041 atggaaagaa atgggttatc agatcaagcc tttaacccaa cttccttaag ttttcgctta
     8101 tccaagcgcc acatcaagta tcatcatcag aacaaagcct gccgccactc cgatcgttcc
     8161 gatattggaa tgttctccgg cctgagattc gggaatgagt tcttctacaa cgacatagat
     8221 catggctcct gccgcaaaag acaaaagata aggaaggaga ggcacggcga ttccggtcag
     8281 aaaaatggtg atcaccgccg caatcggttc tacaatgccg gacattgccc cgtataaaaa
     8341 tgcctttcct tttgacaatc catgactttg cagaggcatg gatataattg ccccttccgg
     8401 aaagttttga attgcaatcc caatcgaaag cgccagcgcg ccggcaagag agattaccga
     8461 attttctgtc attactcccg caaacgtcac tccgaccgcc atcccttccg gaatgttatg
     8521 aagcgttacc gcaaggacaa gcatcgtcgt tttttttaac tgagacggaa ctccttccgg
     8581 cttctcttcc gtaaagtgga ggtgtgggat caatgtatcc aaaacaagaa gaaaacccat
     8641 tccaaataaa aagccgactg ccggaggcat ccatgaaatt cctcccgcct gctctgacat
     8701 gtcaatggcc ggaataagaa gcgaccagcc cagacgccgc gatcattact cccgacgcaa
     8761 atccgagaag cattttctgc aggcggacat tcatttcctt tttcataaaa aataccattg
     8821 ccgaaccaag tgtcgttccg ataaacggaa tcattattcc taataaaata ttactgttca
     8881 tctctattcc tccgattttt atactgccgc ggacaatctt cttcaccata tccaccgccg
     8941 actgtaatat agaattacac cgatacttta tcctatgcat atgcccaaaa ctctgacaca
     9001 tatattcata attcatatat cggatcatat catacgtttc tgcatattgc aattattttg
     9061 atatctattc tcatttcgtc tttttcaacg ctcttcttta tagtaatatt gtttaatttt
     9121 ttttattttt ttatcctttt tttagttgtt tcacacatag aattagtgta taataagttt
     9181 aacacatttg agaggtgata ttctcaaaca atacttacat aaaggagaga ttcgccatgg
     9241 tatatttagt aacaggcccg agaggcagcg gaaaaactca gcagatgatc gatcttgcta
     9301 acgaaaaagt aaaaacatct aacggtaatg tagtatttat caaaaaaagc cataaagata
     9361 cttacacggt tgacttcaat atccgtgtaa tccgcatggc tgattatccg gacgttctaa
     9421 cgctcgaaga attcgtaggt ttcctttacg gtatggcagc cggaaaccat gatatcgaag
     9481 cagtctttat tgacagtgtt ttaaaacagg ctaacatcac tcttgaaagt cttgccgctt
     9541 tccttcacaa attaaacaag atttcaacag aaaacaatat tgatttttat ctcagcatca
     9601 gcgcggataa aaaagatatt cccggaatcg attcatctga ttgtacagtt gtcagctaac
     9661 tgctgcatct atattttatc gacagtcacc tgtacagcaa aacggttcac agcgctattg
     9721 actgtgaacc gttttttaat tcttttattg tattgttttc acttcggtaa gcgacagaac
     9781 cgtattgctc ttttcgcctt ctttatattc tatcgtcacc tgatctccca cgttatagcg
     9841 gataatgtca ataaaatcaa caaccgacac atcaaatata tcgtcagatc cttccaccat
     9901 aatatagtaa tgtgagtttc cttccactac tccctgagca attttcgtaa tcggagccgt
     9961 aatcgtctgt acttccctta cgtcttcctc ttcttcttta ataccgtttt catacattaa
    10021 atctgtataa acgctttcac actcgcttac agtatcgccg atcgcaacga tctgatattt
    10081 ctgaacattt accatcgcat actttttcac aagtcccgca tcatctttaa gtgcaataaa
    10141 atatgtcggt tctcctgaaa tatttaacag taaaggaaac gtcgcaacat atttcaagtt
    10201 ctgtacctgt ccttctgcag aagacatcgc agacgcctct gtcgcacctt caacttcgta
    10261 aaatttagtt tccatcgtac gctgattcat cagaacaaac ccgacattcg actgatcgcc
    10321 tgtgatcgaa gtaactcctg tataaaccca tacatcatcg ttgatcgcca gataattata
    10381 tccgtccgtt gtggcaagac agtctttctg tccgagcacg ctgttaaaat atccatgctt
    10441 caatgttccg taataatcgt ataattgaac aagaagatct gccgaatata cgcgatctac
    10501 ccactgcgga acgtctttga ctttataatc taccgtttct ccggtaactg cgttgcaaag
    10561 cacaacccga tccactgtca caccgccgaa aagaccgata ttatattttt tcacagggca
    10621 aacccaatac ggcgtacctt cttcgtctac ttcaaagctt aaatcattaa aaatatatgt
    10681 cggatactta aatctcaa
//
LOCUS       DBMDXF010000051        10670 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1734_length_10670_cov_2.247327, whole genome shotgun sequence.
ACCESSION   DBMDXF010000051 DBMDXF010000000
VERSION     DBMDXF010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_10670_cov_2.247327"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(171..1313)
                     /locus_tag="ACZPD4_03460"
     CDS             complement(171..1313)
                     /locus_tag="ACZPD4_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIR1378163.1"
                     /translation="MGTEFKDLDTTPTLTLDPFQAADKKETPAVQQVEEPVLDDSILT
                     EEERQTVEAFAKQIDLTNSSLILQYGAGTQKKMADFSESALDNVKSQDLGEVGDLLTG
                     VVTQLKSFDEDEEEKGFFGIFKKASNKIDSMKAKYAKAETNVNEIIKALEKHQVQLMK
                     DTALLDKMYELNLTYFKELSMYILAGKKKLAEVKRTQLADLMSRAQASGLPEDAQAAK
                     DLDEMCIRFEKKIHDLELTRMISIQTAPQIRLVQNNDTMMVEKIQSTIVNTIPLWKSQ
                     MVLALGVEHSTQAAEAQRRVTDVTNELLKKNAEKLKIATTETARQSERGIVDMDTLKA
                     TNESLISTLDEVMAIQREGREKRQAAEAELRNMEQELKGKLLQLHG"
     gene            complement(1453..2973)
                     /locus_tag="ACZPD4_03465"
     CDS             complement(1453..2973)
                     /locus_tag="ACZPD4_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR1378164.1"
                     /translation="MINNEWERSLDELNRTIQDAIDHQNYGRLNQNVTDTIGRAMEGL
                     SRGLRNGIRYGSDAMNAAADNFAQRQRQAGNTPPFHNQGTGSAGGFTQNQGAGSAGGF
                     TQNQRTGAAGGFTQNQRTGATGGFTQNQRTGATGGFTQNQRTGSAGGFTQNQRTGAAG
                     GFTHNQGAGSAGGFAQDQRTGTAGNSVHRGGILYLKGTAIKVGGIFLAITGYLGMIVT
                     LIIGLMLLLGSSVTGWQMEINVMAALMGGFSVAFAVMGIIGTSNACTVSRFGKYVKMI
                     GNREYCDVKEFAQQSGRSVKAVVKDLKKMIKKGWFRQGHLDEQETCLMTSNEAYRQYT
                     ALMERTRREKQEKEERAAKEQEEFSRLSPEVQKIIEAGDEYVRAIKAANDAIPGEEIS
                     EKIARMEMLVDRIFDRVEQKPDTVSDIRKLMDYYLPMTMKLLQAYEDLDAQPVQGENI
                     ISSKIEIEKTIDTLNVAFEKLLDDLFQDTAWDVSSDISVLNTMLAQEGLTEDEIRK"
     gene            3079..3867
                     /locus_tag="ACZPD4_03470"
     CDS             3079..3867
                     /locus_tag="ACZPD4_03470"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1378165.1"
                     /translation="MIKNASPLIIYEDEQIIVCHKPAGTPTQSSHIGTSDMESLLKNH
                     LFSSRKFHRAKSGEPYLAIIHRLDQPVEGILVFAKTPAAAKELNRQLTSQEFGKYYLA
                     VISGIPEPQEGSLENYLVKDARTNSSAVCTKTTPGAKLARLHYRTIETYGTPGNEKFT
                     DSKSTDTPKDTSRNHRQPPLQNESSALVEIHLDTGRHHQIRVQMAHFGHPLIGDRKYG
                     KKITDHMNSVQSSTKRFQAGQLHLCAYKLTFRHPKTGKEMEFQI"
     gene            complement(4018..10335)
                     /locus_tag="ACZPD4_03475"
     CDS             complement(4018..10335)
                     /locus_tag="ACZPD4_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1378166.1"
                     /translation="MKWTKKMKGAGKKAAVTVTALGMVLTAFPPIPAKAAEDFAGQLT
                     SVESVKKGDADNIVIVTFNGGVKAKLTFLEDGIFRYNVDPSGEFSEYAKPRQSSHKGR
                     IQQYPDSSSEYSKPEAKIKDADGKFTITSKNVSVEFDKETAKMKILSNDKVVMEEKEA
                     LAISKSATVQTLKKNDGENFYGGGTQNGRFVHTGESINIVNESGWTDGGVASPNPFYY
                     TTGGYGVLRNTFADGKYDFGKTAADTVTATHEEGELDAYYFVSDGKDNTEKTQELLSE
                     YYHVTGNPVLLPEYAFYEGHLNAYNRDAWSNEKPAGSSKAWTIKGTDPSTSEGKTTYE
                     IGMSDKFVPVDGQQLESLNGEGPTVATENYPEVTTPYEFSARAVLDDYVKYDMPLGYF
                     LPNDGYGAGYGQNGHHMTGGVNADGTSSADRLAAVKANVDNLAKFTEYAQGKGVQTGL
                     WTQSYLTPDSNSGTLWHRLRDFKNEVKVGGVSTLKTDVAWVGHGYSMQLDGVKTAYDI
                     ATTEGDIRPNIISLDGWAGSQRYCGIWTGDQTGGNWEYIRFHIPTYIGTSLSGNPNVG
                     SDMDGIFGGDALIATRDYQWKSFTPLMLNMDGWGTYAKMPYTFGDPYTGINRMYMKIK
                     AQLLPYIYTGAASAANIDTGNGDTGLPLMRAMFLEYPNDSYASSKAMQYQYMLGSEML
                     VAPIYQNTADAENDGNDIRNNIYLPGEKDVWIDYFTGEQYQGGRVLNNFDAPLWKLPV
                     FVKNGAIIPMWEENNSPEEIDKANRIVEFWPDGKSSYTMFEDDGKYIENDLKEEGEYG
                     KVTDASYGDHVSAKFTSEVKDGTATLTAEKSTGNYDGYKKDKNTTFVVNVSKKPSKVT
                     AKNGSETLNVEEVTSKADFDAKKAEKGKAVYFYDESPAIETYASEKETGIADLVKDVK
                     RTPKLYVKFAQADAKAVDQTLVIEGFANDGKLQKDQLNEKLAVPTLTEKTDEKTPTSI
                     TLGWNKVENATSYELMIDDVVFAVGDAEAYTHVDLAYHSEHTYQVRSRNADGYSKWSE
                     KQTFQSAEDPWKDTPKPQSITWSGAIYGNHTADRAFDQEFQQSDGGFHSDGNSIGQTL
                     TVDYGKAYKLDKIEYYPRTDAGNGTVTKMKLETSLDGVHWSEAKEYDWERNAETKVMD
                     KLNCGARYIRFTPLAALGNFFSASEIKVYKEENSTPFAVGSTNNSETVIEADYTNMKN
                     YLGVGGDDKIFIDQIKSRYGDINMNNVYDVYDYAFTMFQLDGGTQKTDSVSGEAMLLA
                     SSDSVKKGETFTVDVYAQDVAGLNAFGQVINYDQSKVEFVSCEISPLLAQMEDLTVNK
                     KYEDRAYVNLAFANRGDKELYTGSDMLATITMKAKEDISTTDAKVIDLSKVTLIGPNY
                     SMIESTVDAEVEIPDVTVTEKKFGQADFNITMTNEILTEDNAEEPNVGKLIQQGNYNG
                     LFNGTINRDFELKWDIESNHDPETGALPEYIKLPLTMHLALKNPEALNKVVVSNANKG
                     NGYLQKASAKLIYTDGTESDEIKIETEQDKYTFEFEGGKAVKEVQITFLQATSTGNKN
                     NMLTLAELELSNLSNTPVTGITADPNNAKEMFVGTLADINAIVQPDNATNKFFTVESS
                     DEDVVKIITLADENKHPIYKARAMKEGKSTITLTAAGNKEVKATYDINVKVGVDISGL
                     NEALAKARTYQASAYTEESYGQLTAAVNAATELLKGEYTKNQVLEAQMAIYAAIDGLT
                     FRPLDETKLLDAKAEGFTVTATSECDPDKLEDGLATNVLDGKEDNYWHTEYNKDVLPQ
                     SLNFDLGGLYNLTDITFLARQGVTNGDILKAQIFVGSDKDDMKSVGTYEFDENGNVLV
                     NRDQYQQIAFDAKDVRYVEFKVLEAGAQDKFASMAEIRFYGEKTTAALKSLYDSYVAE
                     NLDKADYTADSWAVYEAKMNEANALIGANDTTNAAAGEALTALQTAHDRLVKLNPDPQ
                     PGDVDKSGLTTLYNQYKDTKADGYTAESWTAFNEALMKAQSVLANPNATQDEVNAAKE
                     ALQTAYDGLVKDTTPDPNPNPNPDGNGGSGGNSGSNGNSGNSGSNGSNGNGGNAAVVT
                     GDSANIAGYLMALVAAGGIAVVTFFRRKRVK"
BASE COUNT         2436 a         2803 c         1971 g         3460 t
ORIGIN      
        1 ttataaccag tttcccatga gacctctggg gtggaaatca ccacaaactg ttctgaggga
       61 atttattgat cggggtgtaa catatgtttg acaaacctac aaaccgccgt tccaagtatt
      121 cattccgaaa ataccatcct aaacgtgcat ctaagacctt atcccaaaca ttatccgtga
      181 agctgcaaaa gctttccttt taattcctgc tccatattgc gaagttccgc ctccgcagcc
      241 tgacgttttt ccctgccttc acgctggatt gccattactt catcaagcgt agagatcaga
      301 gactcgttcg ttgctttgag cgtatccata tccacgattc cccgctcgga ctgccgagcc
      361 gtttccgtcg ttgcgatctt cagtttctct gcatttttct tcaaaagctc attcgtcaca
      421 tccgtaacac gccgctgcgc ttccgctgcc tgcgtcgaat gttctacccc aagcgcaaga
      481 accatctgac tcttccaaag aggaatcgta tttacgattg tagactggat cttttcaacc
      541 atcattgtat cattattctg aacgagccga atctgaggcg cagtctgaat agaaatcatt
      601 cttgtcagtt caagatcatg gatcttcttc tcaaaccgaa tgcacatctc atccaaatct
      661 ttcgccgcct gcgcatcttc cggcagtcct gatgcctgcg ccctgctcat cagatccgca
      721 agctgtgttc ttttcacttc cgcaagtttc ttctttcccg caagaatata catcgacaat
      781 tccttaaaat atgtcagatt cagttcgtac atcttatcaa gaagcgctgt atccttcata
      841 agctgtacct gatgcttctc cagcgccttg ataatctcat ttacattcgt ctcggctttt
      901 gcgtatttcg ctttcataga atcaattttg ttcgacgctt tcttaaaaat gccgaaaaag
      961 cctttttctt cttcatcttc atcaaaactt ttcaactgcg tcacaacgcc ggtaaggaga
     1021 tcccccacct cgccgagatc ttgagatttc acattatcca acgccgattc ggagaaatcc
     1081 gccatcttct tctgcgttcc cgctccgtac tgaaggatca gcgatgaatt cgtcaagtcg
     1141 atctgctttg caaatgcctc gaccgtctga cgctcttctt ctgtcaaaat gctgtcgtcc
     1201 agcaccggtt cttctacctg ctgcacggcc ggagtttcct ttttatccgc cgcctgaaac
     1261 ggatctaacg tcaatgtcgg agtggtgtct aagtccttaa attctgttcc catttcttta
     1321 atctcctttt ccatttcttt cttcagccct atcttactta tcttcactca tcattccccg
     1381 agcttctatt ttatcttctt tactcgctgc gaacatcttt tctatcttat cttctccact
     1441 cgctgcgcgc ttttactttc tgatctcgtc ttccgtaaga ccttcctgtg ccagcatcgt
     1501 attcagaacc gatatatccg acgacacgtc ccacgccgta tcctgaaaaa gatcgtccaa
     1561 caacttttca aacgccacat taagagtgtc aatcgtcttt tcgatctcta ttttggaaga
     1621 aataatattt tctccctgta caggctgtgc atccaaatct tcatacgcct gcagcagttt
     1681 catcgtcatc ggaagataat aatccattaa ttttcggata tcactgaccg tatcgggttt
     1741 ctgctcgacc cggtcaaata tcctgtctac aagcatttcc atacgggcga ttttctccga
     1801 aatttcttct ccgggtattg cgtcattcgc cgctttgatc gccctcacat attcgtcgcc
     1861 tgcttcgatg atcttctgga cttccggaga aagtctggaa aactcttcct gttccttcgc
     1921 tgcccgctct tctttttcct gtttttcccg acgcgttctt tccataagcg ccgtgtattg
     1981 gcgataagct tcgttgctcg tcataagaca tgtttcctgc tcatcaagat gaccttgccg
     2041 aaaccagcct tttttaatca tctttttcaa atctttcaca actgctttca cagaacgtcc
     2101 gctctgctgt gcaaattctt ttacgtcaca atattccctg tttccgatca ttttcacata
     2161 ttttccaaaa cggctcaccg tacacgcatt cgatgttccg ataattccca tcactgcaaa
     2221 cgcaacagaa aaaccgccca tcaaagccgc catgacattt atctccatct gccagcctgt
     2281 taccgaactt cccaacagaa gcatcagacc gatgatcaga gtaacgatca tcccaaggta
     2341 tcccgtaatc gcgaggaaga taccgccgac ctttattgcc gttcccttca gataaaggat
     2401 tccgcctctg tgcacggaat tccccgctgt tcccgttctt tgatcctgcg cgaagccgcc
     2461 tgctgagccc gctccctgat tatgtgtaaa tccgcccgct gcgcctgtcc tctgattctg
     2521 tgtaaatccg cctgctgagc ctgtcctctg attctgcgtg aaaccgccag ttgcgcctgt
     2581 cctctgattc tgcgtaaaac cgccagttgc gcctgtcctc tgattctgcg tgaatccgcc
     2641 cgctgcgcct gtcctctgat tctgtgtaaa tccgcctgct gagcccgctc cctgattctg
     2701 cgtgaaacca cctgcagatc ctgttccctg attatgaaac ggcggtgtgt ttcccgcttg
     2761 tctctgcctc tgtgcaaaat tatccgcagc ggcattcatc gcatcgcttc cgtaacggat
     2821 tccgtttctt agtcctctgg agagtccttc catcgcacgt ccgatcgtat ccgtcacatt
     2881 ctgatttaac cgtccgtaat tttgatgatc gatagcgtcc tgaatcgtcc gattcaattc
     2941 atccaacgat ctttcccact cattatttat catgaatttc ctccttaaag ggttgacctg
     3001 aaaattcttt tatatgataa tcagtttagc cgtaacacat tgaaaagtca ataatacgaa
     3061 ggagcgaaaa taagacaaat gataaaaaat gcatccccgc ttattatata cgaagacgag
     3121 cagatcatcg tctgccataa acccgcagga acgccgactc agagcagtca tatcggaact
     3181 tccgacatgg aaagcctgct caaaaaccat cttttttctt cacgtaaatt tcaccgggca
     3241 aaatcaggcg aaccgtatct tgcgatcatt catcgcctcg accagcctgt agaagggatt
     3301 ctcgtctttg caaaaacacc tgccgccgca aaggaactaa accgccagct cacttcccaa
     3361 gaatttggga aatattatct tgcagtaata tcaggcattc ccgaaccgca ggaaggctct
     3421 cttgaaaact atctcgtcaa agatgcccgt acaaacagtt ccgcagtctg tacgaaaacg
     3481 actccgggag caaaacttgc cagacttcac tacagaacaa tagaaacata cggcacgccc
     3541 ggaaacgaaa aatttaccga ttccaaatcg actgacacgc caaaagacac gtcccgaaac
     3601 cacaggcagc cgccattgca aaatgaatct tctgccttag tagaaatcca tcttgacaca
     3661 ggcagacatc atcagattcg tgtgcaaatg gcacatttcg gacaccccct tataggcgat
     3721 cggaaatatg gcaaaaaaat caccgatcat atgaattccg tacaatcttc gacaaaacga
     3781 tttcaggcag gacaacttca tctctgcgct tacaagctaa catttcgtca tcccaaaacc
     3841 ggaaaagaga tggaatttca gatttgaaac tccatctctc ataaatccga tcttcctgtt
     3901 tggaaagatc tcttctcctg caatcccgaa ttttaactcg tcgcacaaaa aagacggtgc
     3961 cttacgcgca ccgccttttt tgtttcatat aatcttcatc ttacgaatca gatattttta
     4021 tttcactctt tttcttctaa agaatgttac tacagcgatt ccgcccgctg ccacaagcgc
     4081 catcaaatat cctgcgatgt ttgcgctgtc acctgtaaca acagctgcat tgccaccgtt
     4141 tccgttgctt ccattgcttc cgctatttcc actgtttcca ttgcttccgc tgtttccgcc
     4201 actaccgccg tttccatccg gattcgggtt cgggttcgga tcaggagttg tgtcttttac
     4261 aagaccgtca taagctgtct gcaatgcttc ttttgctgca tttacttcat cctgtgttgc
     4321 attcggattt gcaagaacac tctgtgcttt cataagagct tcattgaatg ctgtccagct
     4381 ttctgctgta tatccgtctg ctttggtgtc tttgtactga ttataaagag tcgtcagtcc
     4441 actcttatct acatcgcccg gctgcggatc tggattaagt ttcacaagtc tgtcatgcgc
     4501 tgtctgcaat gctgtcaaag cttcgcctgc tgctgcgttt gttgtatcgt ttgctccgat
     4561 gagagcgttc gcttcattca tcttcgcttc gtatactgcc catgagtctg ctgtgtagtc
     4621 tgctttatcc aagttttctg ccacatagct gtcatagaga gatttcaacg ctgctgtcgt
     4681 tttctctccg tagaaacgga tctctgccat acttgcaaat ttatcctgtg ctcctgcttc
     4741 aagaactttg aactctacat atcttacatc tttcgcatca aaagcgatct gctgatactg
     4801 gtctctgttt acaagaacat ttccattctc atcgaactca tatgttccga cagatttcat
     4861 atcgtctttg tcgcttccta caaagatctg cgctttcaga atatcgccgt ttgtcactcc
     4921 ctgacgagca aggaatgtaa tatctgtcaa gttgtaaagt ccgccgagat cgaaattgag
     4981 gctctgcggc aatacatctt tgttgtactc tgtatgccag taattatcct cttttccgtc
     5041 aagtacgttt gttgcaagtc catcttcaag tttatcagga tcacattcac ttgttgccgt
     5101 tactgtgaat ccttctgcct ttgcatcaag cagttttgtt tcatcaagcg ggcggaatgt
     5161 aagtccatcg attgccgcgt aaattgccat ctgtgcttca agtacctgat tcttagtata
     5221 ttcgcccttc agcaattctg tagccgcatt tactgctgct gtaagctgtc cataactttc
     5281 ttctgtgtaa gcagatgcct gatatgtacg agctttagca agcgcttcat ttaatcctga
     5341 aatatcaacg ccgactttaa cattgatatc gtatgttgct tttacttctt tgtttcctgc
     5401 tgctgtcaga gtgatcgtac tctttccttc cttcatcgct ctcgctttgt agatcggatg
     5461 tttgttctca tctgccagcg taatgatctt cacaacgtcc tcatcagaac tttctactgt
     5521 gaagaatttg tttgttgcat tgtccggctg aacgattgca ttaatgtctg ccaatgttcc
     5581 gacaaacatc tcttttgcat tgttcggatc tgctgtgatt cctgtaaccg gtgtgttaga
     5641 aagatttgac agttcaagtt ctgcaagtgt gagcatgtta ttcttgttgc ctgttgatgt
     5701 agcctgcaag aatgtaatct gtacttcttt tactgctttt ccgccttcaa actcgaatgt
     5761 gtatttatcc tgttccgtct caatcttgat ctcatcagat tctgttccgt ctgtgtagat
     5821 cagttttgca gatgcttttt gcaaataacc gttgcctttg tttgcgttcg atacgactac
     5881 tttattcaat gcttccggat tctttaatgc aagatgcatc gtcaacggaa gtttgatgta
     5941 ctccggaagc gctcctgtct ccggatcatg attggattca atatcccact taagctcgaa
     6001 atctcgatta attgttccat taaacagtcc gttatagttg ccctgctgga tcaattttcc
     6061 tacgttcggc tcttctgcat tatcctctgt gaggatttca tttgtcattg tgatattgaa
     6121 gtctgcctgt ccgaacttct tctctgttac cgtaacatcc gggatctcaa cttctgcgtc
     6181 aaccgttgac tcgatcatgc tgtagttcgg tccgatcaaa gttactttag agaggtcgat
     6241 cactttcgca tctgttgtag agatatcttc ttttgccttc attgtgattg tagcaagcat
     6301 gtcgcttccg gtatagagtt ctttgtctcc acggtttgca aacgcaaggt ttacatatgc
     6361 tctatcttca tactttttat taactgtcag gtcttccatc tgagcaagga gtggagaaat
     6421 ttcacaggaa acgaattcca ctttactctg atcgtaattg atcacctgtc caaacgcgtt
     6481 caatcctgcg acatcctgtg cgtaaacgtc aactgtaaac gtctcgcctt tcttcacact
     6541 gtcgctgctt gcaagaagca tagcttctcc tgaaacgctg tctgtcttct gcgttcctcc
     6601 atccaactgg aacatcgtga atgcataatc ataaacgtca taaacgttgt tcatgttaat
     6661 atcgccgtaa cgactcttga tctgatcgat gaagatctta tcgtctccgc ctactccgag
     6721 atagttcttc atgttcgtgt agtcagcttc gattaccgtt tcgctgttat ttgtacttcc
     6781 gactgcaaac ggagttgaat tttcttcttt gtaaacttta atttcgcttg cagagaagaa
     6841 attacccaat gccgcaagtg gtgtgaaacg gatataacgc gctccacagt tcagcttatc
     6901 catcactttc gtttcagcat ttctttccca gtcatactct ttcgcctctg accagtgcac
     6961 gccgtcaaga cttgtctcaa gtttcatctt tgtaacagtt ccgttaccgg catccgtacg
     7021 cggataatac tcgatcttat caagtttata cgcttttccg taatctacag taagcgtctg
     7081 tccgattgag tttccatcag agtggaaacc tccgtcgctt tgctggaact cctgatcgaa
     7141 tgcacgatct gcagtatgat ttccgtaaat tgctccgctc catgtgattg actgcggctt
     7201 cggcgtgtct ttccacggat cttctgctga ctggaatgtc tgtttctcac tccacttgga
     7261 atatccgtcc gcgtttcttg agcgaacctg atatgtatgc tctgaatggt aagcaagatc
     7321 tacatgcgta tatgcctcag catcgccaac ggcaaataca acgtcgtcga tcatcagctc
     7381 gtaagatgta gcgttttcta ctttattcca tccaagcgtg atacttgtcg gtgtcttctc
     7441 gtcagttttc tctgtcagtg tcggaactgc aagtttctca ttcaactgat ctttctgaag
     7501 ttttccatcg tttgcgaatc cttcgattac taatgtctga tcaactgctt tcgcgtcagc
     7561 ctgagcgaat tttacataca gcttcggtgt tctcttcaca tcttttacaa gatctgcaat
     7621 gccggtttct ttctcagatg catatgtctc gattgccgga gactcgtcat agaagtaaac
     7681 tgcttttcct ttttctgctt tcttcgcatc aaagtcagct ttgctcgtca cttcttcaac
     7741 attgagagtt tcactgccat tttttgctgt tactttcgac ggcttcttgg atacgtttac
     7801 aacgaatgtt gtgtttttat ctttcttata tccatcgtag tttcctgttg acttctctgc
     7861 tgtcaatgta gctgttccgt cttttacttc cgacgtaaac ttcgcggata catgatcgcc
     7921 gtaagaagcg tccgttactt ttccatactc gccttcttct ttcagatcat tctcgatata
     7981 tttaccgtcg tcttcaaaca tcgtatagga actctttcca tccggccaga actctacgat
     8041 acggtttgct ttgtcgatct cttccggaga gttgttctct tcccacatcg gaatgatcgc
     8101 gccgttcttt acgaacaccg gaagtttcca aagcggagcg tcgaagttgt tgagaactcg
     8161 tccaccctga tactgctcgc ctgtgaagta atcgatccaa acgtctttct ctcccggaag
     8221 atagatgtta tttctgatat cattaccatc attttctgca tctgcagtgt tctgataaat
     8281 cggagctaca agcatctctg atccgagcat atactgatac tgcatcgctt tgctgcttgc
     8341 atagctgtca ttcggatatt caaggaacat cgctctcata agcggaagtc ctgtatctcc
     8401 gttgcctgta tcgatgttcg ctgcggaagc tgctcctgta taaatgtacg gaagaagctg
     8461 tgcttttatc ttcatgtaca tacgattgat acctgtatac ggatcaccga atgtatacgg
     8521 catcttcgca taagttcccc aaccgtccat gttgagcata agcggtgtaa atgatttcca
     8581 ctgataatct ctcgtagcga tgagcgcatc tccgccgaag ataccgtcca tatcgcttcc
     8641 tacgttcggg ttaccgctaa gagacgttcc gatatatgtc ggaatgtgga agcgaatata
     8701 ctcccagttg ccacctgtct gatctcctgt ccagataccg cagtaacgct gtgatcctgc
     8761 ccatccgtca agagagatga tgttcggacg gatatcgcct tccgttgtcg cgatatcgta
     8821 agctgtcttc acaccgtcaa gctgcataga atatccatgt ccaacccatg caacgtctgt
     8881 cttcaatgta gaaacgccgc cgactttaac ttcatttttg aagtcacgga gacgatgcca
     8941 taatgttccc gaatttgaat ccggcgtcag ataactctgt gtccaaagtc ctgtctgcac
     9001 accttttccc tgtgcatatt ctgtaaactt agcaaggtta tctacattcg ccttaactgc
     9061 cgcgaggcgg tctgccgagc ttgttccgtc cgcattcact ccgcctgtca tatgatggcc
     9121 gttctgtccg tatcctgcgc cgtatccgtc gttcggaagg aaatatccga gcggcatatc
     9181 atacttaaca taatcgtcaa gcactgcgcg ggctgaaaat tcatatggtg tcgttacctc
     9241 cggatagttt tcagtagcca ctgtcggacc ttcaccgtta agagactcaa gctgctgtcc
     9301 gtctaccgga acaaacttat cgctcattcc gatttcatat gttgtctttc cttcacttgt
     9361 cgacggatca gttcctttga tcgtccatgc tttcgaactg ccagccggct tctcgtttga
     9421 ccacgcgtca cggttgtatg cgttcaggtg tccctcataa aaagcatatt caggaagaag
     9481 aaccggatta cctgttacat ggtaatactc actcaaaagc tcttgtgtct tttctgtgtt
     9541 atcctttcca tcagaaacga aatagtaagc gtcaagttct ccttcttcat gagttgccgt
     9601 tacagtatct gccgctgtct taccaaagtc gtatttacca tcggcaaatg tatttctaag
     9661 cactccgtat ccgcctgtcg tataatagaa tgggttcgga gatgctacac cgccgtctgt
     9721 ccatccactt tcatttacaa tattgatcga ctctcctgta tgaacgaatc tgccgttctg
     9781 tgtaccaccg ccgtagaaat tctctccgtc gtttttctta agagtctgta ctgttgcaga
     9841 ttttgaaata gccagtgctt ctttttcttc catcacaacc ttatcgttgc ttaagatttt
     9901 catcttagct gtctctttat caaactcaac agaaacattt ttacttgtga tcgtaaactt
     9961 tccatctgca tctttaatct ttgcttccgg ttttgaatac tcactggagc tgtcaggata
    10021 ctgctgaata cgtcccttgt gggaactctg tctcggtttt gcatactccg agaactcgcc
    10081 gctcggatct acgttatatc tgaagatccc gtcttcaaga aacgtaagct ttgcctttac
    10141 accaccgttg aaagtcacaa taacgatgtt gtctgcatcg ccttttttca ctgactcgac
    10201 gcttgtcaac tgcccggcaa aatcttccgc agctttcgcc ggtatcggcg gaaatgctgt
    10261 aagcaccatg ccaagcgccg taacagttac agcagctttc tttcctgctc ctttcatttt
    10321 cttagtccat ttcatctttt tccttctcct ccttttcttc gggcaaattg ttcaaattga
    10381 ttgcatttac cactaagtac tttattatat aattattttg accaaatgta tttatacaaa
    10441 tcattctcgg tttgtatttt ttaacatttg tatcgccttt tttaccggta ttttcgggta
    10501 ttttcaacat atttctaact tttgtcgatt tatctgtact caatttctat caagacaaaa
    10561 tctctctatt agaaaggtaa tatttgcttc tgtatcttca agatcatctg caatttcgga
    10621 aattgttttt cctttttcaa gttttttccg gatgagagca cttttttctt
//
LOCUS       DBMDXF010000052        10468 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1769_length_10468_cov_2.446417, whole genome shotgun sequence.
ACCESSION   DBMDXF010000052 DBMDXF010000000
VERSION     DBMDXF010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_10468_cov_2.446417"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            374..691
                     /gene="rpsJ"
                     /locus_tag="ACZPD4_03480"
     CDS             374..691
                     /gene="rpsJ"
                     /locus_tag="ACZPD4_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR1378167.1"
                     /translation="MASQVMRITLKAYDHQLVDASAKKIIETVKKNGSQVSGPVPLPT
                     KKEVVTILRAVHKYKDSREQFEQRTHKRLIDIVTPTQKTVDALSRLEMPAGVYIDIKM
                     KNK"
     gene            788..1471
                     /gene="rplC"
                     /locus_tag="ACZPD4_03485"
     CDS             788..1471
                     /gene="rplC"
                     /locus_tag="ACZPD4_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR1378168.1"
                     /translation="MKKAILATKVGMTQIFNYEDGVLIPVTVLQAGPCVVTQVKTVEN
                     DGYSAVQVGYVDKKDKIVTKDGSGRKAIAHRNGVTKAEKGHFDKAGVSGKRYVKEFRF
                     DNAEEYALAQEIKADIFAEGDHIDATAISKGKGFQGAIKRHGQSRGPMTHGSKFHRHA
                     GSNGAASDPSKVFKGKKMPGQMGNKKITVQNLEIVRVDAENNLLLVKGSVPGPKKCLV
                     TIKETVKGN"
     gene            1501..2121
                     /gene="rplD"
                     /locus_tag="ACZPD4_03490"
     CDS             1501..2121
                     /gene="rplD"
                     /locus_tag="ACZPD4_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR1378169.1"
                     /translation="MAKVSVYNIEGKEVDTIELNDAVFGVEVNEHLVHMAVVNQLANN
                     RQGTQKAKTRSEVSGGGRKPWRQKGTGHARQGSTRAPQWTGGGVVFAPVPRDYSFKMN
                     KKEKRAALKSALSSRVEENKFIVVDEMNFDEIKTKKFQNVLNNLNVNKALVVLEDGNT
                     NAEISARNIAGVKTARTNTINVYDILKYNTVIATKAAVATIEEVYA"
     gene            2121..2420
                     /gene="rplW"
                     /locus_tag="ACZPD4_03495"
     CDS             2121..2420
                     /gene="rplW"
                     /locus_tag="ACZPD4_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR1378170.1"
                     /translation="MANIQYYDVILKPVITEKSMDLMADKKYTFLVHTEATKAQIKEA
                     VEKMFEGTKVARVNTMNLEGKNRRRGMVVGKTAKTKKAIVQLTEDSKEIEIFEGL"
     gene            2489..3331
                     /gene="rplB"
                     /locus_tag="ACZPD4_03500"
     CDS             2489..3331
                     /gene="rplB"
                     /locus_tag="ACZPD4_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR1378171.1"
                     /translation="MGIRTYNPYTPSRRQMTGSDFSEITKTTPEKSLLDSKKRKAGRN
                     AQGKITVRHRGGGAKKKYRIVDFKRKKDGIPATVVGIEYDPNRSANIALICYADGEKA
                     YILAPEGLKDGMTVMNGADAEVRVGNCLPLSEIPVGTQIHNIELYPGKGGQLVRSAGN
                     SAQLMAKEGKYATLRLPSGEMRMVPIICRASIGVVGNGEHNLINVGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGKTGIGRPGPCTPWGKPALGLKTRKKNKASNKLIVRRRDG
                     KAIK"
     gene            3358..3639
                     /gene="rpsS"
                     /locus_tag="ACZPD4_03505"
     CDS             3358..3639
                     /gene="rpsS"
                     /locus_tag="ACZPD4_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR1378172.1"
                     /translation="MARSLKKGPFADESLLKKVDAMNAAGDKSVIKTWSRRSTIFPSF
                     VGHTFAVHDGRRHVPVYVTEDMVGHKLGEFVATRTYRGHGKDEKKSKVR"
     gene            3671..4057
                     /gene="rplV"
                     /locus_tag="ACZPD4_03510"
     CDS             3671..4057
                     /gene="rplV"
                     /locus_tag="ACZPD4_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR1378173.1"
                     /translation="MAKGHRSQIKRERNEQRDTRPSAKISYARISVQKACYVLDAIRG
                     KDVQTALGILAYNPRYASAVIEKLLKSAIANAENNNGMSVDNLYIEECYANKGPTMKR
                     IRPRAQGRAYRIEKRMSHITIVLNER"
     gene            4067..4723
                     /gene="rpsC"
                     /locus_tag="ACZPD4_03515"
     CDS             4067..4723
                     /gene="rpsC"
                     /locus_tag="ACZPD4_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1378174.1"
                     /translation="MGQKVNPHGLRVGVIKEWDSKWYAEKDFADNLVEDHEIRTFLKK
                     RLYSAGISKIEIERASDRVKIIVYTAKPGVVIGKGGSEIEKVKAELAQFTDKKLIVDI
                     KEIKRPDKDAQLVAENIAQQLENRISFRRAMKSCMSRTMKAGALGVKTSVSGRLGGAD
                     MARTEFYSEGTIPLQTLRADIDYGFAEADTTYGKVGVKVWIYKGEVLPTKADKEGRDK
                     "
     gene            4723..5160
                     /gene="rplP"
                     /locus_tag="ACZPD4_03520"
     CDS             4723..5160
                     /gene="rplP"
                     /locus_tag="ACZPD4_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR1378175.1"
                     /translation="MLMPKRVKRRKQFRGTMKGKALRGNTIAYGEYGIVAAEPCWIRS
                     NQIEAARVAMTRYIKRGGKVWIKIFPDKPITAKPAETRMGSGKGTVEYWAAVVKPGRV
                     MFEIAGVPEETAREALRLAMHKLPCKCKIVSRADLEGGDNSEN"
     gene            5150..5350
                     /gene="rpmC"
                     /locus_tag="ACZPD4_03525"
     CDS             5150..5350
                     /gene="rpmC"
                     /locus_tag="ACZPD4_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR1378176.1"
                     /translation="MKTNTFVKELRGKSVEELNQELVAAKKELFNLRFQNATNQLDNT
                     SRIKEVRKNIARIQTMITEAGR"
     gene            5375..5653
                     /gene="rpsQ"
                     /locus_tag="ACZPD4_03530"
     CDS             5375..5653
                     /gene="rpsQ"
                     /locus_tag="ACZPD4_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR1378177.1"
                     /translation="MIERRIEVEERNLRKTRTGKVVSDKMDKTIVVAVEDHVKHPLYK
                     KIVKRTYKLKAHDENNECKIGDTVKVMETRPLSKDKRWRLVEIMEKAK"
     gene            5676..6044
                     /gene="rplN"
                     /locus_tag="ACZPD4_03535"
     CDS             5676..6044
                     /gene="rplN"
                     /locus_tag="ACZPD4_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR1378178.1"
                     /translation="MIQQESRLKVADNTGAKELLCIRVLGGSTRRYASIGDVIVATVK
                     DATPGGVVKKGDVVKAVVVRSVKGARRKDGSYIRFDENAAVIIKDDLNPRGTRIFGPV
                     ARELREKHFMKIVSLAPEVL"
     gene            6059..6370
                     /gene="rplX"
                     /locus_tag="ACZPD4_03540"
     CDS             6059..6370
                     /gene="rplX"
                     /locus_tag="ACZPD4_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR1378179.1"
                     /translation="MSTMKIKTGDTVKVIAGKDKDKEGKVIAVNKKDGRVLVEGVNML
                     TKHTKPSAANQNGGIVHQEGYIDASNVMLMHKGQATRVGFKMDGDKKVRFAKKTGEVI
                     D"
     gene            6394..6933
                     /gene="rplE"
                     /locus_tag="ACZPD4_03545"
     CDS             6394..6933
                     /gene="rplE"
                     /locus_tag="ACZPD4_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR1378180.1"
                     /translation="MSRLKEQYQNEIVDAMIKKFGYKNIMEVPKLDKVVINMGVGEAK
                     ENAKVLESAIADMEKIAGQKAVVTRAKNSVANFKIREGMPIGCKVTLRGEKMYEFVDR
                     LINLALPRVRDFRGVNPNAFDGRGNYALGIKEQLIFPEIEYDKIDKVRGMDVIFVTTA
                     KTDEEARELLKQFNMPFAK"
     gene            6946..7131
                     /locus_tag="ACZPD4_03550"
     CDS             6946..7131
                     /locus_tag="ACZPD4_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR1378181.1"
                     /translation="MAKTSMKIKQQRKPKFSTREYSRCRICGRPHAYLRKYGICRICF
                     RELAYKGQIPGVKKASW"
     gene            7220..7621
                     /gene="rpsH"
                     /locus_tag="ACZPD4_03555"
     CDS             7220..7621
                     /gene="rpsH"
                     /locus_tag="ACZPD4_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR1378182.1"
                     /translation="MTMSDPIADMLTRIRNANTAKHDTVDVPASKMKLAIANILLDEG
                     YIAKYDLVEDGHFQTIHITLKYGADKNEKVITGLKRISKPGLRVYANTEDIPRVLGGL
                     GTAIISTNKGVVTDKEARKLGVGGEVLCFVW"
     gene            7801..8343
                     /gene="rplF"
                     /locus_tag="ACZPD4_03560"
     CDS             7801..8343
                     /gene="rplF"
                     /locus_tag="ACZPD4_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1378183.1"
                     /translation="MSRIGRLPIAIPAGVTVEVAENNVVTVKGPKGTLTRELPTEMEI
                     KVEGEEVTVTRPNDLKKMKSLHGLTRTLINNMVVGVTEGYQKVLEVNGVGYRASKSGN
                     KLTLNLGYSHPVEMIDPEGVETVLEGQNKITVKGIDKEKVGQYAAEIRDKRRPEPYKG
                     KGIKYADEVIRRKVGKTGKK"
     gene            8361..8729
                     /gene="rplR"
                     /locus_tag="ACZPD4_03565"
     CDS             8361..8729
                     /gene="rplR"
                     /locus_tag="ACZPD4_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR1378184.1"
                     /translation="MVSKKSRSEIRRKKHMKLRNRFSGTAERPRLAVFRSNNHMYAQI
                     IDDTVGNTLVAASTLQKDVKAELEKTNNVEAAACLGKVIAEKAKEKGITEVVFDRGGF
                     IYQGKIKALADAAREAGLNF"
     gene            8745..9254
                     /gene="rpsE"
                     /locus_tag="ACZPD4_03570"
     CDS             8745..9254
                     /gene="rpsE"
                     /locus_tag="ACZPD4_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR1378185.1"
                     /translation="MRQERIDASQMELLEKVVSIKRVTKVVKGGRNFRFTALVVVGDG
                     NGHVGAGLGKATEIPEAIRKGKEDAMKKMIEITFDENGSITHDVLGKFGSASVLLKKA
                     PDGTGVIAGGPARAVIEMAGIKNIRTKSLGSNNKQNVVLATIEGLKDVKSPEDVARLR
                     GKSIEEIMG"
     gene            9314..9496
                     /gene="rpmD"
                     /locus_tag="ACZPD4_03575"
     CDS             9314..9496
                     /gene="rpmD"
                     /locus_tag="ACZPD4_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR1378186.1"
                     /translation="MANLKVTLVKSTIGAIPKHKRTVEALGLKKTGKTVVLPDNAATR
                     GMVQQVRHLVKVEEEA"
     gene            9517..9957
                     /gene="rplO"
                     /locus_tag="ACZPD4_03580"
     CDS             9517..9957
                     /gene="rplO"
                     /locus_tag="ACZPD4_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR1378187.1"
                     /translation="MDLSNLRPADGSKQSDNFRRGRGHGSGNGKTAGKGHKGQKARSG
                     ATRPGFEGGQMPLYRRIPKRGFTCRNSKTIVGINISELERFEDGATVSVETLIESGIV
                     KNPRDGVKILGNGELTKKLTVQANAFSAKAAEKIEALGGKAEVI"
     gene            9957..>10468
                     /locus_tag="ACZPD4_03585"
     CDS             9957..>10468
                     /locus_tag="ACZPD4_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR1378188.1"
                     /translation="MLKTVRKAFQIEDIRKRIFYTFVMLVIVRLGSQLPTPGVDPTFI
                     KEFFAQQTGDAFNFFEAFTGGSFTNFSIFALSITPYITSSIIMQLLTIAIPKLEEMQK
                     EGEEGRKKIAAITRYVTVALALIESTAMAVGFGRQGLLEEYNFVSCAVVVCTLTAGSA
                     FMMWIGERIT"
BASE COUNT         3669 a         1922 c         2605 g         2272 t
ORIGIN      
        1 atgacatctg ttgtttccat atataatagg gaaaaagaga aaaatgatat tgtattgaaa
       61 aaaatacaat ttttcataaa aatagcttga aatacaatgt tatttcctat atactaaaaa
      121 agctgtgaca tgatagctgt gaagcgtgag gttgctgccc ggcaaaaaga taagctgagg
      181 caggttttcc gtggagcgaa tgtcaagtta ggaaactggc gacaagtcac tgtacgaatc
      241 acaactgatt actgattacc acaagggaaa agtatgaaac aacgtgtgag tttctcacga
      301 cacacacggg agagtgtaca gtcaccgctt gtcgtactga agtcaaaagt acgaaaagga
      361 ggcgactttt tttatggcaa gtcaagtaat gagaatcaca ttgaaagcgt atgatcatca
      421 gctcgttgat gcatccgcaa agaaaatcat cgaaactgta aaaaagaatg ggtcacaggt
      481 gagcggaccg gtgccgctgc cgacaaagaa agaggtagtg acaattctta gagcggtaca
      541 caagtacaaa gactcaagag agcagttcga gcagagaact cataagagac tcatcgatat
      601 cgttacaccg acacagaaaa ctgtggatgc tctttccaga ctggaaatgc cggcaggtgt
      661 ttacatcgat atcaaaatga aaaataagta agcgaaaaac cgctgaacat tcaaattaac
      721 agtaacaatc ctaacattta ggatgagcgc gaaagcgttc cgctgtaaat cacaggaggt
      781 atttataatg aagaaagcta ttttggctac aaaagttgga atgactcaga tcttcaatta
      841 cgaagacgga gttctgattc cggtaacagt tctgcaggct ggaccgtgtg tggttacaca
      901 ggttaaaaca gttgagaacg atggctacag cgccgtgcag gttggatatg ttgacaagaa
      961 agataagatt gtcacaaaag acggaagcgg aagaaaagcg atcgcacaca gaaacggtgt
     1021 gacaaaagct gaaaaaggcc actttgacaa agcaggcgta tccggaaaaa gatatgtaaa
     1081 agaatttaga tttgacaatg cagaggaata tgctcttgct caggagatca aagctgatat
     1141 cttcgcagag ggagatcaca tcgatgcgac agcaatctcc aaaggtaaag gtttccaggg
     1201 cgcgatcaaa cgtcacggac agagcagagg acctatgaca catggttcca agttccatcg
     1261 tcacgctggt tccaatggtg cggcatctga tccgagtaaa gtattcaaag gcaaaaaaat
     1321 gcctggacag atgggtaaca agaagattac tgttcagaac cttgaaatcg tgagagttga
     1381 tgcagagaac aacctgcttc tcgtaaaagg ttcagttccg ggaccgaaga aatgtcttgt
     1441 aacgatcaaa gaaacagtaa aaggaaacta agctgacaat aagaaaggag gaacacacag
     1501 atggcaaaag tatctgttta caatatcgaa ggtaaagaag ttgatacaat cgaattaaac
     1561 gatgctgtat tcggtgttga agtaaatgag cacctcgtac acatggcagt tgtaaatcag
     1621 cttgcaaaca atcgtcaggg aacacagaaa gccaaaacac gttctgaggt ttcaggcggc
     1681 ggaagaaaac cgtggagaca gaaaggaaca ggacacgcaa gacagggttc aacaagagct
     1741 ccgcagtgga caggcggcgg tgttgtattc gctccggttc cgagagatta ctcattcaag
     1801 atgaataaaa aagagaagag agctgctctt aaatccgcac tttcttccag agtagaagag
     1861 aacaaattca tcgtagttga cgagatgaac ttcgatgaga tcaaaacaaa gaaattccag
     1921 aatgtcctga acaacctgaa cgtaaacaaa gcgcttgttg ttttggaaga cggaaataca
     1981 aacgcagaga tttctgctag aaacattgct ggcgttaaga cagcacgcac aaatacgatc
     2041 aacgtatatg acatcctgaa atacaacaca gtgatcgcaa cgaaagctgc tgttgcaaca
     2101 atcgaggagg tgtacgcata atggcaaaca ttcagtatta tgatgtaatc cttaaaccgg
     2161 ttatcacaga gaaaagcatg gatctgatgg ctgacaagaa atatacattc cttgtacaca
     2221 cagaggctac aaaagctcag atcaaagaag ctgttgaaaa aatgtttgaa ggaacaaagg
     2281 ttgctcgtgt caacacaatg aaccttgaag gaaaaaacag aagacgcgga atggtagtcg
     2341 gaaaaacagc taagacaaag aaagcgatcg tacagcttac agaagacagt aaagaaatcg
     2401 aaatctttga aggactgtaa gatcagaaac tatatacggt gcaaatccgt gaaacaaata
     2461 attagacgaa atgaaaggag agaaagtaat gggaatcaga acatataacc catatacacc
     2521 ttccagaaga cagatgacag gatctgattt ctccgagatc acaaagacaa ctccggaaaa
     2581 atcacttctt gattccaaaa aaagaaaagc aggtcgtaac gcacagggta agattactgt
     2641 aagacatcgc ggcggcggcg ctaagaaaaa atatagaatc gtagacttca agagaaagaa
     2701 agacggaatc ccggcaacag tagtaggtat cgaatacgat ccgaacagat ctgcaaacat
     2761 tgcgctgatc tgctacgcag acggagaaaa agcatatatc cttgctcctg aaggactgaa
     2821 agacggaatg acagttatga acggtgcaga tgccgaagta agagtaggaa actgcctccc
     2881 gctttctgag attccggtag gtactcagat ccataacatc gagctttatc cgggaaaagg
     2941 cggacagctt gttcgttcag caggaaacag cgcgcagctt atggctaaag aaggaaaata
     3001 tgcaacactc agacttccgt caggtgagat gagaatggtt ccgattatct gccgcgcatc
     3061 tatcggtgtg gtaggaaacg gagagcacaa cctgatcaat gtaggtaaag caggacgtaa
     3121 gcgtcacatg ggcatccgcc cgacagtacg tggttctgtt atgaacccga acgaccatcc
     3181 gcatggtggt ggtgaaggta agacaggtat cggccgcccg ggtccgtgta caccgtgggg
     3241 taaaccggca cttggtctta agacccgcaa gaaaaacaaa gcttctaaca agctgattgt
     3301 aagaagaaga gacggtaaag cgattaaata attagattca caaggaggtt agaacctatg
     3361 gctcgctcac ttaaaaaagg accatttgca gacgaaagct tactgaaaaa agtagacgct
     3421 atgaacgctg caggtgataa atcagttatt aagacatggt cacgccgttc tacaatcttc
     3481 ccgtcatttg taggccacac atttgccgta catgacggaa gaagacatgt accggtatat
     3541 gttacagaag atatggtagg acacaaactc ggtgagttcg ttgcgacaag aacttacaga
     3601 ggacacggca aagacgagaa gaagtcgaaa gtcagataag attcatatat tttgaaagga
     3661 gggttcatcc atggcaaaag gacacagatc ccaaatcaag agagaaagaa acgagcagag
     3721 agatacaaga ccttcagcta agatttctta cgcaagaata tctgttcaga aggcatgcta
     3781 cgttcttgat gccatcagag gtaaggatgt acagacagca ctcggtattt tagcttacaa
     3841 tccgagatat gcttcagcag ttatagagaa attactgaaa tcagcgattg ctaacgctga
     3901 gaacaacaac ggtatgagcg ttgacaacct ttacattgag gagtgctacg caaacaaagg
     3961 accgacaatg aagagaatca gaccgagagc acagggtaga gcttatagaa tcgagaagag
     4021 aatgagccac atcacaatcg tgctgaatga aagataggag gacaatatgg gacagaaagt
     4081 aaatcctcat ggcttgagag tcggcgttat caaagaatgg gattccaaat ggtacgcaga
     4141 gaaagatttc gctgacaact tggttgaaga ccacgagatc agaacatttt taaagaaaag
     4201 attatacagc gccggaatct ccaaaatcga gatcgagaga gcttccgatc gcgtgaagat
     4261 cattgtttat acagctaaac cgggcgttgt tatcggtaag ggcggttccg agatcgaaaa
     4321 agtaaaagca gagcttgctc agttcacaga taagaaactt atcgttgata tcaaagagat
     4381 caaaagaccg gacaaagacg cacagcttgt agctgagaac attgcccagc agcttgagaa
     4441 ccgtatttca ttcagacgtg ctatgaaatc ctgcatgtcc agaacaatga aagcaggagc
     4501 tcttggagtt aaaacttctg tatccggacg tctcggcgga gctgatatgg ctcgtacaga
     4561 gttctacagc gagggaacaa ttccgcttca gacacttaga gcagacattg actatggatt
     4621 cgcagaagct gacacaactt acggaaaagt cggtgtaaag gtttggattt acaaaggcga
     4681 agttcttccg actaaagcag ataaggaagg gagagataaa taatgttaat gccaaaaaga
     4741 gtaaaacgtc gtaaacagtt ccgtggtacc atgaaaggaa aagctctcag aggtaacacg
     4801 atcgcatatg gtgagtacgg tatcgtagcg gcagagccgt gctggattcg ttccaatcag
     4861 atcgaggcag cccgtgttgc catgacacgt tacatcaaac gtggcggtaa agtttggatc
     4921 aaaattttcc cggataagcc gatcacggca aaaccggctg agactcgtat gggtagcggt
     4981 aaaggtacag ttgagtattg ggcagctgta gtaaaaccgg gccgcgtgat gtttgaaatt
     5041 gcaggagtac cagaggaaac agctagagag gcacttcgac ttgcaatgca taaattacct
     5101 tgcaaatgca aaatcgtttc tcgcgcagac ttagaaggcg gtgataacag tgaaaactaa
     5161 tacatttgta aaagaattaa gaggaaaatc cgtagaggaa ttaaatcagg aattagtagc
     5221 tgctaagaag gaactcttca acttgagatt ccagaatgcc acaaaccagt tggacaatac
     5281 aagcagaatc aaagaagtaa gaaaaaatat cgcacgtatc cagacgatga tcacagaggc
     5341 tggacgataa gcggatatca gcttctttgc cagaatgatt gaaaggagaa tagaagtgga
     5401 agagagaaat ctgagaaaaa cccgtacggg taaggtcgta agcgacaaga tggacaaaac
     5461 aatcgttgtt gccgtggaag accatgtaaa acacccgctt tataagaaga tcgtaaaaag
     5521 aacttacaag ctgaaagctc atgatgagaa taacgagtgt aagatcggtg atacagtaaa
     5581 agttatggaa acaagacctc tgtcaaaaga caaaagatgg agacttgtgg agatcatgga
     5641 gaaagcaaaa taatttttga aggaggttta cctgcatgat acagcaagaa agcagactga
     5701 aagtagcaga caacacaggc gctaaagaat tactgtgcat ccgtgtgctt ggcggttcta
     5761 caagaagata tgcgagcatc ggtgatgtga tcgttgcaac tgtaaaagat gcaacaccag
     5821 gcggcgttgt taaaaaaggc gatgtcgtaa aagctgttgt tgttcgttct gtaaaaggcg
     5881 ctcgtcgtaa agacggttcc tatatcagat tcgatgaaaa tgctgctgtt attataaaag
     5941 acgatttgaa tccgcgtgga acacgtattt ttggaccagt agcccgtgag cttagagaga
     6001 agcactttat gaaaattgtt tctcttgctc cggaagtact gtagggaggt gcctaagaat
     6061 gtcaactatg aaaattaaaa caggcgacac tgtaaaggtt atcgccggaa aagataaaga
     6121 caaagagggc aaagttatcg ccgtaaataa aaaagacgga agagtcctcg ttgaaggcgt
     6181 caatatgctg acaaagcaca caaagccgag cgcagcaaac cagaacggtg gcatcgtaca
     6241 tcaggaaggt tatatcgatg catctaacgt aatgcttatg cacaaaggtc aggcaacacg
     6301 agtaggcttc aaaatggatg gagacaaaaa agttcgtttc gcaaagaaga ctggcgaagt
     6361 tattgattaa ttcgggaagg aggaccaagg actttgagta gactgaaaga acagtaccag
     6421 aatgagatcg ttgacgcaat gatcaaaaaa ttcggatata aaaatattat ggaagtgccg
     6481 aagctcgaca aagttgttat caacatgggc gttggcgaag caaaagaaaa tgcaaaggtt
     6541 ttagagtctg caatcgccga tatggaaaag atcgcaggtc agaaagccgt tgttacaaga
     6601 gcaaagaact ccgtggctaa cttcaaaatc agagagggta tgccgatcgg atgtaaggtt
     6661 acgcttagag gcgagaaaat gtatgagttc gttgatcgtc tgattaacct tgcgcttccg
     6721 cgtgtgcgtg acttcagagg tgtaaacccg aacgcatttg acggaagagg aaactatgca
     6781 cttggaatca aggagcagtt gattttcccg gagatcgaat atgataagat cgacaaagtc
     6841 agaggtatgg acgtaatctt tgttacaaca gccaaaactg acgaggaagc acgtgaatta
     6901 ttgaaacagt tcaatatgcc gtttgcaaaa taaaggagga aaatcatggc aaagacatct
     6961 atgaaaatca aacagcagcg caagcctaaa ttctccacaa gagaatatag ccgctgcaga
     7021 atctgcggac gtccgcatgc gtatttgaga aaatatggaa tctgccgtat ctgcttccgt
     7081 gaactggcat acaaaggaca gatcccggga gttaagaaag ccagctggta agccgaaagc
     7141 agtttggcgc atcgcaaaga aattttccgc ggggcagaaa atgtagaaca gatagaatat
     7201 aaaaggagga tacatccaca tgactatgag cgatcctatt gcagatatgc ttacaagaat
     7261 ccgtaatgcg aacactgcaa aacatgatac agttgatgta ccggcatcca agatgaaact
     7321 tgctatcgca aatattcttc ttgatgaggg atatatcgca aaatatgatc ttgtagaaga
     7381 cggacacttt cagacaattc atatcacact gaaatacggt gctgataaga acgagaaagt
     7441 tatcacagga cttaagagaa tctcaaaacc gggacttcgt gtttatgcaa acacagaaga
     7501 tatcccgaga gtactcggcg gacttggaac agcgatcatt tctacaaaca aaggcgttgt
     7561 aacagataaa gaagcaagaa aactcggcgt aggcggagag gtactttgct tcgtttggta
     7621 atttaaatcc gttgagtaca gaggaaacac catcgccgct aaactcgtgg gagacctcgt
     7681 taagcgtcga ggtgtacttt cgcactaagc tcgaaagtac ctcgctaagt gctcaatgca
     7741 actgaaaaca gaggagccaa agcagctcct ccgaaaatct aagttaagga ggaatagggt
     7801 atgtcacgta taggaagact gccgattgca attcctgcag gagtaactgt agaagttgca
     7861 gagaacaatg tagtgactgt gaaaggtccg aaaggaactc ttacaagaga gcttccgaca
     7921 gaaatggaaa ttaaggttga aggcgaagaa gtaactgtaa caagaccgaa cgatctcaag
     7981 aaaatgaaat ctcttcacgg tttgacaaga acactgatca acaacatggt tgtcggagtt
     8041 acagaaggat atcagaaagt ccttgaagta aacggtgtag gttacagagc atcaaaatca
     8101 ggaaataaac tgacactgaa ccttggatat tctcatccgg ttgagatgat cgatccggaa
     8161 ggcgttgaga cagttctgga aggtcagaac aagatcactg taaaaggaat cgacaaagaa
     8221 aaagttggac agtatgcggc tgagatcaga gataagagaa gaccggagcc atataagggc
     8281 aaaggtatta aatatgctga cgaagtaatc agacgtaaag taggtaagac cggtaagaaa
     8341 taaataagga gagtgtgaaa atggttagta agaaatcaag aagcgaaatt cgtagaaaaa
     8401 aacacatgaa attacgcaac cgtttcagcg gcactgctga aagaccgcgt ttagctgttt
     8461 ttagaagtaa taatcatatg tatgctcaga tcatcgacga cactgttgga aatactctgg
     8521 ttgcggcttc cactcttcag aaagatgtga aagcagaact tgagaaaaca aacaacgttg
     8581 aagcagcagc atgcctcgga aaagtaattg ccgagaaagc aaaagaaaaa ggtattacag
     8641 aagttgtatt cgacagaggc ggcttcattt atcagggtaa gatcaaagca ctggcagacg
     8701 cagcaagaga agccgggctg aatttctagg aagggagaac acagatgaga caggaacgca
     8761 ttgatgctag tcagatggaa ttattagaaa aagtggtatc aatcaaacgt gttaccaaag
     8821 ttgttaaagg tggtcgtaac ttcagattta cagctttagt tgttgtcgga gacggcaatg
     8881 gtcatgttgg tgcaggtttg ggaaaagcta ctgaaatccc ggaagcgatc cgcaaaggga
     8941 aagaagacgc tatgaagaaa atgatcgaga tcacattcga tgagaacgga agtatcacac
     9001 acgatgtact cggaaaattc ggaagtgcat ccgtacttct gaaaaaagct ccggatggta
     9061 caggagtaat cgccggaggt ccggctcgtg ccgtgatcga gatggcggga atcaaaaata
     9121 ttcgtacaaa atctctcgga tcaaacaata aacagaatgt cgtacttgct acgatcgaag
     9181 gactcaaaga cgttaagtct ccggaagatg ttgcaagact tcgcggcaaa tcgatcgagg
     9241 agatcatggg ctaagacgat agaaaatgtt gaataacaga ttctatccta tcgcataagg
     9301 aggtaagatt gaaatggcaa acttaaaagt tacgttagta aagtctacaa tcggagccat
     9361 accaaagcat aaaagaacag ttgaggctct tggtctcaag aagaccggca agacagtcgt
     9421 actgccggac aatgcagcta ccagaggtat ggtacagcag gttagacatt tagttaaggt
     9481 tgaagaagag gcataaaaag ataaggaggt aacgtcatgg acttatcaaa cttaagaccg
     9541 gcagacggtt caaaacagag tgataatttc agaagaggcc gtggacatgg ttcaggaaac
     9601 ggtaagacag ccggtaaagg ccataaggga cagaaagcac gttccggcgc tacaagaccc
     9661 ggattcgaag gcggtcagat gcctttgtac agaagaatac cgaagagagg attcacttgc
     9721 agaaactcta agacgatcgt aggtatcaac atcagcgaac tcgaaagatt cgaggacgga
     9781 gctacagtat ctgttgaaac actgatcgag agcggtatcg tgaaaaaccc gagagacgga
     9841 gttaaaattc ttggaaatgg agagttaact aagaagctta cagttcaggc aaatgcattt
     9901 agtgcaaaag ctgctgagaa aattgaagcg cttggtggaa aagcagaggt gatctaatgc
     9961 tgaagacagt gcgtaaagca tttcagattg aggatatcag aaaacgaatt ttttatacgt
    10021 tcgtaatgct tgtcatcgta agacttggat cacagcttcc gactccgggg gtagatccga
    10081 catttatcaa ggaatttttt gcacagcaga cgggggacgc tttcaacttt tttgaagcgt
    10141 tcaccggagg ctcgtttaca aacttttcca tttttgcatt gagcatcaca ccgtacatca
    10201 cttcatccat tattatgcag cttctcacaa tcgcaattcc gaaattagag gagatgcaaa
    10261 aagaaggaga agaaggaaga aagaagattg ccgcaattac acgttatgtg acagttgcgc
    10321 tggcgcttat tgagtctact gcaatggcag ttggattcgg tcgacagggt cttctcgaag
    10381 aatacaattt cgtaagctgt gcagttgtcg tatgtacact gacggcggga agtgccttta
    10441 tgatgtggat cggtgagcgc atcactga
//
LOCUS       DBMDXF010000053        10082 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1852_length_10082_cov_2.065825, whole genome shotgun sequence.
ACCESSION   DBMDXF010000053 DBMDXF010000000
VERSION     DBMDXF010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1852_length_10082_cov_2.065825"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="ACZPD4_03590"
     CDS             complement(<1..283)
                     /locus_tag="ACZPD4_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR1378189.1"
                     /translation="MTQHNEKGTRRDLVLYRLEVAKSDIKSAKILLDAGECRGANNRA
                     YYGIYHAISAIHALDGNAYKRHKDALANFNKNYIKTEIFPRILGRKIAEA"
     gene            complement(270..605)
                     /locus_tag="ACZPD4_03595"
     CDS             complement(270..605)
                     /locus_tag="ACZPD4_03595"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR1378190.1"
                     /translation="MTTRELVSQYVEVLCKIYGKHLKTVILYGSYARGDYTKDSDIDI
                     MVLLDLSDIDIKKYRHELSGATYDFNMDYDVDIKPIAKNQEHFNKWVEVYPFYSNVAS
                     EGVKLFDAA"
     gene            complement(979..4779)
                     /locus_tag="ACZPD4_03600"
     CDS             complement(979..4779)
                     /locus_tag="ACZPD4_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1378191.1"
                     /translation="MKKKMWRTGLCGLTACSMLFTMPALAWAADEPQNTNIDNGLIAY
                     YDFENVNEKKVPNVKDQSKYEGELKGSNVSIASDTIFGKSLKFTEGTEGTMEIPQIMN
                     TSQNSYSISLWFKYDTNTNREGKNTVLLQQSGQGRTFLQFTKDNKYATYVNATNVYSD
                     KTVDLSQWQHVMATYNKDTKKIAFYINGEKDSEKDAGNQVVNELTNLLIGRHKNAGND
                     PLSMRGLVDEIRVYDKVLTAEEAKAVYESKAGAMLFPQLQETLKEAKELDASGSLDTE
                     AEEAKVLKEAIAEAEKLTAESQLSEISETIKELEEAMKNYRAAIGVVLTVSPTKEERS
                     IEKSTIGINHRYAFNGYGSFDSTKMEMKEEFTDLYKEAGFGSIRYPGGTISNLFRWKD
                     TIGDKEDRVNQIHGFYNNPNQGGIAPNFGLTEVADFAYRDDVQSEIVYVYGFGRGSAQ
                     DAADLVEYLNAPAGSNPGGGVAWADIRKENGHAEPYNVRYFEIGNENNQPGTDGTTSQ
                     QYWMIGTQDAEKAYVEGGVASFTKQYAVKKDDWNKAASVSDGTANQVRYMRYANPNPM
                     TGKDGKTLVENFEAVQKGSVEVWVGTDGEGNNHKWEVVESLDNAGANDQKVTIDYRDG
                     SIHFGDGTHGKIPAKGQQIYVTYKVKRDGFVAVSKAMKDMTAEINEINAKSGSAEKAS
                     CYVYSSWETKGFIDKMAAGNWNDYYDGLTIHPYCGDPGADQDKGAFYDSAMRLAENVG
                     IQKVKNYVNMLPQGKVPVISEYGIFRSTSPLLRSQTHAVYIAKVLMEYVRLGSPYIQK
                     HCLVDWYSSGADSLGPTQQAVIQAVPQTGANQGTGEGNFRFFSTPSAKVFELFSNSFA
                     KGTKVVGTEFEHVETLANGTKAYSAIASKGDDGTLYVTVVNTDRENDKRLRVKVDGVD
                     LTGKTVEVQTLAGESFVDENSLAEPDKVTIENSTVTAEGTDLELTAKAHSVMSITVKE
                     KVDPPAPETYTVTAKANNTDMGTVTIDPVKDKYNAGEKVTATATAKEGYEFVNWTVDG
                     QEVSTKTTYELTVEKNTELTANFKAKAPVEEKFTVTARVNDSKMGEAKIDPDKAEYVK
                     GEKVKAIATAKEGYEFVNWTVDGEAVSTEATYEFTVDKNVELTANFKETEKPVEKFTV
                     TAKVNDSKMGTVKLDPAKESYENGEIVTAIATAKEGYKFVNWTVAGKVVSDKAEYKFK
                     VDRAAELQANFVKVSEDPKPENPKDEDKAVQTGDNGVSPIIPLAGLMLAAGAAVVALR
                     KKED"
     gene            complement(5131..6075)
                     /locus_tag="ACZPD4_03605"
     CDS             complement(5131..6075)
                     /locus_tag="ACZPD4_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1378192.1"
                     /translation="MENLIFSLNATVPVFLLMALGFLFRKLGWLDEVFASKMNQFVFL
                     VPLPLLVFEDLASVDFQKVWNLKFVLFCFFVTLASIALAGILSLLWKNRGIRGEFIQA
                     SYRSSAALLGIAFIQNIYGDAGLAPLMIIGSVPLYNMMAVVVLSFFQPEQRKRDKLLW
                     KKTLKGIVTNPIIIGIAAGLFWSALRIPMPYVIEKTVSNIGAVATPLGLMALGASFDV
                     QKALGKIKPVIAACMLKLVGFTAVFLPFAVMLGFRREELIAILVMLGSATTVSCYVMA
                     KNMGHEGTLTSGVVMLTTVFSAFTLTGWLFILKSMNLV"
     gene            complement(6180..9788)
                     /locus_tag="ACZPD4_03610"
     CDS             complement(6180..9788)
                     /locus_tag="ACZPD4_03610"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1378193.1"
                     /translation="MLQIKEICKEYRTGSLVQKALDKVSLNLRDNEFVAILGPSGSGK
                     TTLLNVIGGLDRYDGGDLIINGISTKKYKDRDWDSYRNHTIGFVFQSYNLIPHQTLLA
                     NVELALTISGVGKAQRRERARQALEKVGLGEQIHKRPSQLSGGQMQRVAIARALVNNP
                     DILLADEPTGALDSETSIQVMELLKEVAQDRLVVMVTHNPELAQQYATRIVELKDGKI
                     RSDSDAFYVDEETADVPEHKNMGKSSMSFLTALSLSFHNLKTKKARTLLTSFAGSIGI
                     IGIALILSLSAGVNDYIKNIERETLSQYPLQIQSTGFDFTSMMTGGEDSEEVKAVSAG
                     TGTSSKERREKENIWVIDMVTNMFSTMDSNDLESLKKFLDSKESRIDQYTNAVEYSYQ
                     VVPQIYAQDGEKVRQVHPDRSFEAAGLGSSVGSNSLMSSMMSTDVFYQMPADSDLYKD
                     QYDVKAGRWPKSYNECVLVLTSGGGMSDLLLYTLGLRDPLELEEMVAGFVEEEQIEVP
                     KDSTVYTYDDILGKEFKLVNSVDYYEYDNSYHVWKDRSGDQTYMDKVVKNAEPIRIVG
                     IVQPVKDANGAALMSGINYPASLTKHVAEEAENSKIVKDQKADPKRNVLTGEHFGTEN
                     VKGNVNMDSLITIDEQKLARAFAFDQNAFSGMQKTDGLSEIFRSAGSSVNLSGMLDLS
                     KIQVQLPELPQMNLEELLGDLDLTVSPEGFLPLAESLTEGYAEYAAAHPEADYSALAQ
                     QFGQYLGTPKAQEIIRRNISEIIQSGGGMSVTMEQLQAMVKEIAEGYGKFIVENGYQD
                     PSKFDEYLAEYMQTEEAKAILNRYANEIFGGIAGNVQITAEQLQKLSKELTEGYLLYA
                     AENGLADPTKIGEHFIAYLKTEDGRQRLSDGMKGIVDADSLEQQITDAFGFYMKGAVS
                     SYAGAVGTAIERQITSAMELVMNQMAEGLQFSVGQAMTQIGSSLQNAMHVDEDAFAEA
                     FQMNMTGEELAELMMSMGAAKNATYDNNLKQLGYVDFAVPTTISIYPKDFESKEEVVR
                     ILDDYNRKMEEQGKEEQVITYTDMVGALMSSVTQIIDVISYVLVAFVAISLIVSSIMI
                     GVITYISVLERKKEIGILRAIGASKSNISQVFNAETFIIGLCAGVIGIGITLLLLIPG
                     NALIHHLAGTNDVSAVLPVIPAIILIILSVILTLLGGLLPSKKAAKSDPVTALRTE"
BASE COUNT         2505 a         2567 c         1709 g         3301 t
ORIGIN      
        1 cagcctcagc aattttcctt cccaaaattc ttgggaagat ttctgtcttg atataatttt
       61 tattaaaatt tgcaagggca tccttatgcc tcttatatgc gtttccatct aaagcatgaa
      121 tagctgaaat agcatgataa attccataat aggctctgtt attcgctccc ctgcattctc
      181 ctgcatctaa aagaattttt gctgatttta tatcactttt agcaacttcc aacctatata
      241 gaaccaaatc tcgccttgtg cctttttcat tatgctgcgt caaataactt taccccctca
      301 cttgccacat ttgaataaaa cggatacact tctacccact tattaaaatg ctcttgattc
      361 tttgctatcg gcttaatatc tacatcataa tccatattaa aatcataggt tgcaccagat
      421 aactcatgac gatacttttt aatgtctata tcacttaaat ccaaaagcac cataatatct
      481 atatccgagt cctttgtata atcgcccctt gcataagaac catacagaat aactgttttc
      541 aaatgttttc cgtatatttt acagagcact tcaacatatt gactcactaa ctctcttgtt
      601 gtcatagata taccacctct tttaccaact ctctattatt aaaaatcata ctttccaata
      661 ccttaaattt gtattttatg aacattatcc tttattccac gaaatcaatc gttcataagc
      721 attcatttca aatatctatc catttttatt tgctccacca gtcaaccttc ttatacttat
      781 tataaccgat ttttatttct tttcctatct ttatttgata cctgattata aatatgtagc
      841 agttcagccc gcgccattcg ttatacagaa agagacgccg catagcgcgg cgcctctggt
      901 tttgtttcat tttttgcttt ttatcaagac aatctttatg ctatccgatc tgtttttcag
      961 actgttcaaa tattcagatt agtcttcttt tttgcgaaga gctacgactg ccgctcctgc
     1021 cgctaacatc aaccctgcaa gcggaatgat cggtgatacg ccgttgtctc ctgtctgaac
     1081 tgctttatcc tcatccttcg gattctccgg tttcggatct tctgacactt ttacaaagtt
     1141 tgcttgcagc tccgctgctc tgtcaacctt aaacttgtac tctgctttat cagaaactac
     1201 tttgccggct actgtccagt tcacaaactt gtatccttct tttgctgtcg caatcgctgt
     1261 tacaatttcg ccgttttcat agctttcttt tgcaggatca agttttaccg ttcccatctt
     1321 agagtcattt acttttgctg ttactgtaaa cttctctacc ggtttttctg tctctttgaa
     1381 attcgctgtc agttccacgt tcttatctac tgtaaattca tatgtagctt ctgttgaaac
     1441 tgcttcaccg tctactgtcc agtttacaaa ctcatagcct tcttttgctg tcgctatggc
     1501 ttttactttc tctcccttaa catattctgc tttgtcagga tcaattttcg cctctcccat
     1561 cttagagtcg tttactctcg ctgttactgt aaacttctct tctaccggag cttttgcttt
     1621 aaagttagct gtcagttctg tgttcttttc tactgtaagt tcatatgtag tttttgtaga
     1681 aacttcttga ccgtctactg tccagtttac aaactcatag ccttcttttg ctgtcgctgt
     1741 tgctgttact ttttcgcctg cattgtattt atcttttaca gggtcaatcg ttacagttcc
     1801 catgtccgta ttgtttgctt ttgctgttac tgtatatgtc tccggtgcag gcggatccac
     1861 tttttctttc actgtaattg acattacgga gtgtgctttt gccgtcagct caagatctgt
     1921 gccttctgcc gtaactgttg agttttcaat cgttacttta tccggctctg ccaagctgtt
     1981 ttcatcaacg aagctttcac ccgcgagcgt ctgtacttct actgttttgc ctgtcaggtc
     2041 tacgccgtct actttcacgc gaagtctctt atcattttca cggtctgtat ttactacagt
     2101 tacatacaac gttccgtcat cacctttact tgcgattgcg gaatatgctt ttgttccgtt
     2161 tgccaatgtt tctacgtgtt caaactctgt tccgactact ttcgttcctt ttgcaaatga
     2221 gttagagaat aactcgaaca cttttgctga aggtgttgag aagaaacgga agttcccttc
     2281 tcctgttccc tgattagcgc ccgtctgcgg tactgcctgg ataaccgcct gctgtgtagg
     2341 tccaagtgag tctgcaccac tactatacca gtctacaaga cagtgtttct ggatgtacgg
     2401 actaccgaga cgaacatact ccataagcac ttttgcaata tatactgcat gtgtctgaga
     2461 acgaagaagc ggcgatgtag atctgaaaat accatactca gatataaccg gaacttttcc
     2521 ttgcggaagc atatttacat aatttttaac tttctgaata cctacatttt ccgcaagacg
     2581 cattgccgag tcataaaacg cacctttgtc ctgatccgca cccggatctc cacaatatgg
     2641 atgaattgtc agaccatcat aataatcgtt ccagttacct gctgccatct tatcaataaa
     2701 accttttgtt tcccaactgg agtatacata gcaggatgcc ttttctgcac ttccgctttt
     2761 agcattgatt tcgttaatct ctgccgtcat atctttcatc gctttagaaa cagctacaaa
     2821 tccgtctctt ttaactttat atgttacata gatttgttgt cccttcgccg ggattttacc
     2881 atgtgttccg tctccgaagt ggattgatcc gtctctgtag tcgattgtca ctttctggtc
     2941 attcgctcct gcgttgtcaa gactttctac aacctcccat ttatgattgt ttccttcacc
     3001 gtcagtgcct acccatactt caacgcttcc tttttgaaca gcttcaaagt tctcaaccaa
     3061 tgtctttcca tcttttcctg tcatcggatt cgggtttgca taacgcatgt aacgaacctg
     3121 atttgcagtt ccgtcactga cagatgctgc cttattccag tcatcttttt tcaccgcata
     3181 ctgttttgta aagcttgcaa ctccaccttc tacatacgct ttttcggcat cctgcgttcc
     3241 tatcatccaa tactgctgag atgttgttcc atcagtaccc ggttgattat tttcatttcc
     3301 aatctcaaag taacgtacgt tgtacggctc tgcatgtccg ttttcttttc ggatatctgc
     3361 ccatgcaaca ccaccgcccg gattacttcc tgccggagcg ttcaaatact ctacaagatc
     3421 tgcagcatcc tgtgcagaac cacgtccgaa accgtataca taaacaatct ctgactgaac
     3481 gtcatcacga tatgcaaaat cagcaacttc tgtcaaaccg aagttcggag cgattccacc
     3541 ctgattcgga ttgttgtaaa agccatggat ctgatttaca cggtcttctt tatccccaat
     3601 cgtatctttc caacggaaca ggttcgagat cgttcctccc ggataacgga tagatccgaa
     3661 tcctgcttct ttgtagagat ccgtgaactc ttctttcatt tccatctttg tactgtcaaa
     3721 agagccatat ccgttgaaag catatcgatg gttaatacct atcgtcgact tttcaatcga
     3781 acgttcttct ttcgtaggtg aaactgtaag tacaactccg attgccgcac ggtaattctt
     3841 cattgcctct tccagttctt taattgtctc tgaaatttca gacaactgac tttcggcagt
     3901 taatttttcc gcttctgcaa tcgcctcttt cagcactttt gcctcttctg cttctgtgtc
     3961 caaacttcct gatgcatcta actcttttgc ttctttaagc gtttcctgta actgcgggaa
     4021 gagcattgct cctgcttttg attcataaac agcttttgcc tcttctgctg tgagaacttt
     4081 atcatataca cggatttcgt ccacaagacc tctcatagaa agcgggtcat ttcctgcatt
     4141 tttatgacgc ccgatcaaaa ggtttgtcaa ctcattaaca acctgattac ctgcatcttt
     4201 ctcgctatcc ttttctccgt ttatataaaa cgcaattttc tttgtatctt tattgtatgt
     4261 agccattaca tgctgccact ggctaagatc aaccgtctta tcgctatata cattcgtcgc
     4321 atttacataa gtcgcatact tattatcttt cgtaaactgt aaaaatgtac gcccctgtcc
     4381 gctctgctga agcagaactg tatttttccc ttctctattt gtattcgtat catatttgaa
     4441 ccacaaagaa atcgaataac tgttctgaga cgtattcata atctgaggga tttccatagt
     4501 accttcagtt ccctctgtaa atttcaggga tttcccaaaa atcgtatctg atgcaatcga
     4561 aacatttgaa cctttcaatt caccctcata ttttgactga tcttttacgt tcggaacttt
     4621 cttctcattc acattctcaa agtcataata cgcaatgagt ccgttatcaa tgttcgtgtt
     4681 ttggggttca tctgccgccc atgccaacgc cggcatagtg aacaacatgc tgcacgctgt
     4741 caatccgcac aaccccgtcc tccacatttt ctttttcata ctttttctcc attctacgat
     4801 actgtttttt ctgctattgc taaactgtct tttggttttt ctcctcccac gaaagaccag
     4861 tattgtttat attctatcat aaattaattt tttttaaagt tttttatgca tttttcctta
     4921 tagattagag taaaaacgga attttttatg atttcccgta ataatatgtg cctattttcg
     4981 tttgttttat gtgagttttg tacacaaaac acaactgatg caatgcgctc ctaatctcac
     5041 acagataatt ttatactgta tgagaccaac ttcaggtttt ccaaagagaa gcggatacga
     5101 agtttcactt tataaggaaa ccattcttat tcataccaaa ttcatacttt ttaaaatgaa
     5161 cagccaccct gttaatgtaa acgcactgaa tactgtagtc agcatgacga cgcctgacgt
     5221 cagcgtgcct tcatgtccca tattttttgc cataacatag cagcttactg ttgtcgccga
     5281 gccaagcatg acgagaatcg cgatcaattc ttctctgcga aatccgagca tcaccgcaaa
     5341 aggcagaaac accgctgtaa atccgacaag cttcaacata caggcagcaa tgacaggttt
     5401 gatctttcca agcgcttttt gaacatcaaa agaagcgccg agcgccatca atccaagagg
     5461 cgtcgcgaca gcgcctatat ttgacactgt tttttctatt acatacggca tcggtatccg
     5521 aagcgccgac cagaaaagac ctgcggctat gccgattatg attggattgg tcacgatccc
     5581 tttcagcgtc tttttccata acaacttatc tcgttttctt tgttccggct gaaagaagga
     5641 caggacgacg actgccatca tgttatataa cggcacgctt ccgatgatca taagaggcgc
     5701 cagacccgca tctccgtata tattttggat gaatgcgatc ccaagaagcg ccgcgctgct
     5761 tctgtatgac gcctgtataa actctcctct tattcccctg ttcttccata ataacgaaag
     5821 tataccggca agagcaatgc ttgcaagagt cacgaagaaa caaaataaaa cgaattttaa
     5881 attccacact ttctgaaaat cgacagatgc aagatcttca aacacaagaa gcggaagcgg
     5941 cacaagaaag acgaactgat tcattttcga tgcaaatact tcgtcgagcc atccgagttt
     6001 ccgaaagaga aacccaagcg ccattaacag gaaaaccggt actgtcgcat ttaaactaaa
     6061 gattaaattt tccataatca aaaatcactt ctccatcgct tttatagttc atatcaagat
     6121 tttctataca tacacagccg caagcattgg ttatctgtct tgattgctta tgcaagaaat
     6181 tattctgtcc gaagggcagt tacaggatca cttttcgcag cttttttaga cggaagcagt
     6241 cctccgagca gcgtcagtat tacgctgaga ataataagta tgatcgccgg aattaccgga
     6301 agaaccgcac ttacgtcatt cgttcctgcc aaatgatgga tcagagcatt tcccgggatc
     6361 aaaagcagaa gcgttatccc gatcccgatc acgcccgcac acaaaccgat aataaacgtc
     6421 tcggcattaa atacttgtga aatattactt ttagaagctc cgattgcgcg cagtatcccg
     6481 atttctttct ttcgctcaag aacactgata taggtgatca caccgatcat gatcgatgat
     6541 acgataaggg aaatcgctac aaacgcaacg agcacataac tgataacgtc tatgatctgt
     6601 gtcacagacg acatcagcgc gccgaccata tccgtatatg tgataacctg ttcttctttt
     6661 ccttgttctt ccatttttct gttataatca tcaagaattc tgacgacttc ttctttgctt
     6721 tcaaaatctt tcggatagat cgaaatcgta gtcggaacag cgaaatcaac ataacctaac
     6781 tgtttgagat tattgtcata cgtcgcattt ttcgcagctc ccatagacat catcagttct
     6841 gccagttcct ctcctgtcat attcatctga aacgcctcgg caaacgcatc ttcatctaca
     6901 tgcatagcat tttgcagact gcttccgatc tgtgtcattg cctgtcctac agaaaactgc
     6961 agtccttctg ccatctgatt cataaccagc tccatagccg atgtgatctg cctctcaatc
     7021 gctgttccta cggctcccgc ataagaggaa accgcgcctt tcatataaaa accgaacgcg
     7081 tctgtgatct gctgttccag actgtccgcg tctacgattc ctttcatccc gtcagacagt
     7141 ctttgccgtc cgtcttccgt ttttaaatat gcaataaaat gctctccgat ctttgtcgga
     7201 tcagccagcc cgttttccgc cgcatagaga agataccctt ccgtgagctc ttttgaaagc
     7261 ttctgaagct gttctgcggt aatctgaaca ttacctgcaa tacctccgaa aatctcattt
     7321 gcatatcggt tcaatatcgc tttcgcttct tctgtctgca tatattccgc caaatattca
     7381 tcaaacttag acggatcctg atagccgttt tcaacgataa attttccgta tccttccgct
     7441 atttctttca ccattgcctg aagctgttcc atcgtcacgg acatcccgcc gcccgactgg
     7501 atgatctcac tgatatttct tcgtatgatt tcctgcgctt ttggcgttcc aaggtattgt
     7561 ccgaactgtt gtgcaagcgc agaatagtcc gcctccggat gcgcggccgc atattctgcg
     7621 tacccttctg tcaggctttc tgccagcggc aaaaatcctt ccggcgaaac tgtaagatca
     7681 aggtctccca gcaattcttc cagattcatc tgcggcagtt cgggaagctg aacttgaatc
     7741 ttacttagat ctaacattcc cgaaagattg actgaacttc cggcagaacg gaaaatctca
     7801 gaaagaccat ccgttttttg cattcccgaa aaggcatttt gatcaaatgc aaatgcccgg
     7861 gcaagttttt gttcgtcgat cgtaatcaga gaatccatat taacgttgcc ctttacattt
     7921 tctgtcccga aatgttcccc tgtaagaacg tttcgtttcg gatctgcctt ctgatctttg
     7981 actatttttg agttttccgc ttcttctgcg acatgttttg tcaatgaagc gggataattg
     8041 atgcctgaca tcaaagctgc tccgtttgca tcttttacag gctgaacgat gccgacgatc
     8101 cggatcggct cggcattttt cacaactttg tccatgtacg tctgatctcc cgaacgatct
     8161 ttccacacat gataactgtt gtcatactca tagtaatcca cactgtttac cagtttaaac
     8221 tcttttccga gaatatcatc atatgtatag acagttgaat ctttcggaac ttcgatctgc
     8281 tcttcctcga cgaatcccgc aaccatctct tccagctcca acggatctct taagcccagc
     8341 gtgtacagca aaaggtcgct catacctccg ccggacgtca gcacaagtac gcattcattg
     8401 tatgatttcg gccacctgcc ggctttcaca tcgtactgat ctttatacag atcggaatct
     8461 gccggcatct gataaaagac gtctgtactc atcatcgaag acatcagact gtttgagccg
     8521 acagacgaac caagccctgc cgcctcgaaa gagcggtccg gatgtacctg acggactttc
     8581 tctccgtctt gcgcatagat ctgcggaaca acctgataag aatactcaac tgcatttgtg
     8641 tattgatcga tccggctttc tttactgtct aaaaactttt tcaaagattc cagatcattt
     8701 gaatccattg tcgaaaacat attcgtgacc atgtcgatca cccatatatt ttctttttcc
     8761 cgccgctctt ttgaagatgt tcccgttccc gcagacaccg cttttacttc ttccgaatct
     8821 tccccgcctg tcatcatcga agtaaaatca aatcctgtac tttggatctg aagcggatat
     8881 tgggaaagcg tttctctttc tatatttttt atgtagtcat tcactcctgc ggacaaagag
     8941 agaataagcg caatgccgat aattccgatc gatccggcaa aagaagtcaa taatgttctc
     9001 gcttttttcg tcttgagatt atgaaagcta agtgaaagcg ccgtcagaaa cgacatggat
     9061 gatttcccca tatttttatg ttccggaacg tctgccgttt cttcatccac ataaaatgca
     9121 tcgctgtccg aacgaatctt tccgtctttt aactcaacga ttcttgtagc atactgctgc
     9181 gccagctcgg gattatgagt taccataaca acaagcctgt cctgcgcaac ctctttaaga
     9241 agctccatca cctgaatact tgtctcgctg tcaagcgccc ctgtgggttc atcggcaagg
     9301 aggatatccg ggttatttac aagcgctctt gcaatggcaa ctctttgcat ctgtccgccg
     9361 gaaagctgac tcgggcgctt gtggatctgc tcgcctaagc caactttctc caacgcctgt
     9421 ctcgcacgtt ctctgcgctg cgctttgcct actcccgaaa tcgtaagcgc cagttccaca
     9481 tttgccaaaa gtgtttgatg agggatcaga ttataacttt gaaacacaaa accgatcgtg
     9541 tgattccgat acgaatccca gtcccggtct ttgtatttct tcgtcgaaat cccgtttatg
     9601 atcaagtctc cgccgtcata cctgtccaat cctccgatga catttaacag cgtcgttttc
     9661 cccgaaccgc ttggtcctaa aatcgccacg aactcattat cacgcagatt caggctcact
     9721 ttatcaagcg ctttttgaac aagacttccc gtccggtatt ccttacatat ttcttttatc
     9781 tgaagcatac tgttttcgtt cctttccgtg tcttaaatct atcgtcatct tccaaacaat
     9841 atctcataca ccctatatga cagactttct gcagtgaact accattgtct gaagccaagg
     9901 ggcttcctgt tttcagaata ctaagattaa tgatagtcca aaaccctgcc tctttcaaga
     9961 taattttcct ttgaaatccc gccttgtctt tcatcttcta ccagcctttt aagaaaaaca
    10021 atcctgctcc caatcctttt cctatggctg cccccaatca atcggttttc agcttatcca
    10081 gc
//
LOCUS       DBMDXF010000054        10024 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1868_length_10024_cov_2.052605, whole genome shotgun sequence.
ACCESSION   DBMDXF010000054 DBMDXF010000000
VERSION     DBMDXF010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_10024_cov_2.052605"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(221..3766)
                     /gene="nifJ"
                     /locus_tag="ACZPD4_03615"
     CDS             complement(221..3766)
                     /gene="nifJ"
                     /locus_tag="ACZPD4_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1378194.1"
                     /translation="MARKMKTMDGNQAAAHVSYAYTEVAAIYPITPSSVMPEHVDEWA
                     TEGRENIFGTTVEVTEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNLYKVAG
                     EQLPGVFNVSARALASHALSIFGDHSDVYACRQTGAAMLCESSVQEVMDLTPVAHCAA
                     LEGKLPFINFFDGFRTSHEIQKIETWDYEDLKDMVNMDAIDEFRAHALNPNHPCLRGS
                     AQNPDIFFQAREACNPYYDALPGIVQNYMDKVNEKLGTNYKLFNYYGAEDAEHVIVAM
                     GSVCDTIEETIDYLTAAGEKVGVVKVRLYRPFSAEALIDAIPDSVKKISVLDRTKEPG
                     ALGEPLYLDVVAALKGSKFDAVPIYTGRYGLGSKDTTPAQIVAVYHNDEKAKFTLGIV
                     DDVTNLSLKADEPLVTTPEGTINCKFWGLGADGTVGANKNSIKIIGDNTDMYAQAYFD
                     YDSKKSGGVTMSHLRFGKSPIKSTYLIHQANFVACHNPSYVDKYNMVQELVDGGTFLL
                     NCPWDMEGLEKHLPGQVKAYIANHNIKFYTIDGIKIGKEIGLGGRINTVLQSAFFKLA
                     EIIPEEEAISLMKAAAKATYGRKGDKIVQMNYDAIDAGAKQVVEIEVPESWKDAADEG
                     LAVPHIDENGRKDVIDFVKNIQTKVNAQEGNSLPVSAFTDYADGSTPSGSSAYEKRGI
                     AVDIPIWQPDNCIQCNRCAYVCPHAVIRPVALTEEEAANAPEGMQSIPMIGMPDMKFA
                     ITVSAYDCTGCGSCANVCPGKKGEKALVMGNMEENAGKQTFFDYGREIPVKPEVVAKY
                     KETTVKGSQFKQPLLEFSGACAGCGETPYAKLITQLFGERMYIANATGCSSIWGNSSP
                     STPYTVTPEGKGPAWSNSLFEDNAEFGYGMLLAQNTIRNRLKGLVEKLAADAENEDVK
                     AAAQEYLDTYTCGATNGTATDKLVAALEACGCDRAEKAELLKNKDFLAKKSQWVFGGD
                     GWAYDIGYGGVDHVLASGKDINIMVFDTEVYSNTGGQSSKATKTGATAQFAAGGKETK
                     KKDLAGMAMSYGYVYVAQIAMGADFNQTVKAITEAEAYPGPSLIIAYAPCINHGIKKG
                     MSKAQTEEQLAVECGYWNNFRFNPGAEGDKFFLDSKEPKKEDYQAFLDGEVRYNALKR
                     ANPEKAEKLFAINEQEAMERYAYLKKLVDVYKAEE"
     gene            complement(4080..4937)
                     /gene="lgt"
                     /locus_tag="ACZPD4_03620"
     CDS             complement(4080..4937)
                     /gene="lgt"
                     /locus_tag="ACZPD4_03620"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1378195.1"
                     /translation="MHTEISFPNLGIYLKNVGKSIDLFGIEIAYYGIIIGTAILLGFW
                     IAAREAKRTGQNPENYLDMGIIGVIAGIVGARLYYVIFSWDMYKDNLLDIFNLREGGL
                     AIYGGVIAAVISVLVLAKVKHLSAPQIFDTIAMALLNGQMLGRWGNFFNREAFGGYTD
                     SLFAMRLPLDAVRSSDVTEQMRRHIERIDGVSYIQVHPTFLYESLWCMVLLIILFAYR
                     KHKKYEGELFLMYLFGYGLGRFWIEGLRTDQLLLPGVGIAVSQLLAGAIVVGTGAAML
                     YLRKKHKNS"
     gene            complement(5117..6214)
                     /gene="ychF"
                     /locus_tag="ACZPD4_03625"
     CDS             complement(5117..6214)
                     /gene="ychF"
                     /locus_tag="ACZPD4_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1378196.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGAESANYPFCTIDPNVGVVTV
                     PDKRLDVLGEMYHTKKIIPAAIEFVDIAGLVKGASKGEGLGNQFLANIREVDAIVHVV
                     RCFENTNIVHVDGSIDPIRDIETINLELIFSDLEILERRIAKTVKLSRNDKAAAKELD
                     LLNRVKAHLEDNKMAKSFVTDDEDEQEWLASYNLLTAKPVIFAANVSEDDLADDGANN
                     EGVKAVREYAASEDCEVFVVCAEIEEEISQLDDDEKAMFLEELGLKESGLEKLIRASY
                     HLLGLISYLTAGEPEVRAWTIKRGTKAPQAAGKIHTDFERGFIRAEVVSYDDLMACGT
                     HAAAKEKGLVRLEGKEYVVQDGDIMLFRFNV"
     gene            complement(6457..9942)
                     /locus_tag="ACZPD4_03630"
     CDS             complement(6457..9942)
                     /locus_tag="ACZPD4_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HIR1378197.1"
                     /translation="MRVKRRKTMALLAAVSIVCAQSTVSDVFAAGAEEVAAQENNAVS
                     IEYLTLQPGERDDSVNLNWYAPEGTQNAIVRMGDKTVEASVRALHTPTKVVEGKYTDT
                     GKIVCQATVSGLSKGTEYSYQISYDNGNTWSKKYSYETPNAGEFRFGFTSDPQIKENG
                     ETNNGGWNPSDGKNQTGWAVMLDKMKEEDVNLIVSAGDQVEDQSWGKSSEYAAFFAPE
                     EMTSLLYAPAVGNHDRHYMFDDHFNLPNEMSVDGNAASLNQVKTTFRGQNSGTSQSHG
                     NYIQATADEIAQSAESNGVTPNKDGQYDFVERREMETKGNYYYLYNNVLFVTLNTGAY
                     PGGNDNENAGNVNVPSASKDNSEAEAIVSNFRTTLNAATAEYSGQYQWLVVTHHKSTQ
                     TVAKHAADSDIENYVDAGFEQVMDEFDVDFVLGGHDHVYSRSYVLKNGQRNAERLDDF
                     HDPDGVIYLTGNCCSDMQYYTPFEKVDKTNNADYPVLANGQTGSEAYLAGNLPIGNQE
                     WNQEYSPSYAVFDVADNKISVNVYNLSGDSNAPESKLIDSFSVTKNANGGELKNGLEN
                     KKSSIAMTQTAQYNSGMQNPDGGVMEIIDYNTVTGWAYAVNGVTGNLTAIPMKNKNAV
                     DKIALLDGKNINIKEIVENNYKGFSYGDMTSVAVSPNGEKLAVAIQSSDYSVAGCLAV
                     FLCGADGTLSFDQIYEAGVQPDMVTFTPDSKKILSADEGEPRNGYEAGSIDPKGTVTV
                     IDLTNQNVSHLDFTVFDSEAKRAELVQNGVILKKGTAPSVDLEPEYIAANDKTAYVTL
                     QEANAIAIVDLQTLSIENICSAGYEDYEKYPIDIDKKDAAYRPVSYPSLRGIRMPDGI
                     SLFESNGKTYIVTANEGDSREWNEYLNEAECNFGKGQTSPSGKITAENSGLTGKVVFF
                     DQNDYEGLNSEYDYLFGGRSFTVYCVDGSGMKEVYTSGNELEAKTAAYFPQYFNCSND
                     SAEIDDRSGKKGVEAESVTIGTVGEKTYAFIGLERIGGVMAYDITNPDKILFANYINS
                     RDFSKDIAGDVSPEGLCMISASESADGNAYLLASCEVSGTVAAYKLISQNIDSSDDDN
                     TDNNHDNNIDHNGSGHGGADTEDLNNQESKTNALKTGDHAPVIGTGIGMVLALSAIIV
                     ILKYRRSKNTITNTK"
BASE COUNT         2488 a         2523 c         1872 g         3141 t
ORIGIN      
        1 gtgtttcgat ggattatgcg attatattga tgatttttaa ttttatcaag agtatttccg
       61 agttcttagc aagtattctg ataattattt gtgcggttaa gtatctgaaa aaacattgac
      121 tgatgaaaat caacaggaca gcggcttttt ctgccgctgt cctgtcattt aaaaacgatc
      181 ttttaactgc ctttcaatcg tcttttatct tgtgtatatt ttactcttct gctttgtaca
      241 catctacaag ttttttcaga taagcatatc tttccatagc ttcctgctca ttgattgcga
      301 acagtttttc tgctttttcc ggatttgctc tcttaagtgc attgtaacgt acttctccgt
      361 cgaggaatgc ctgataatct tctttcttcg gctctttact gtcgaggaag aatttatctc
      421 cttctgctcc cggattgaag cggaagttat tccagtatcc acattctacc gcaagctgct
      481 cctctgtctg agctttgctc atacctttct tgattccgtg gttgatacac ggtgcataag
      541 cgataataag tgacggtccc ggataagcct ctgcctctgt aatcgctttc actgtctggt
      601 tgaagtcagc tcccatagca atctgcgcta catatacata gccgtagctc attgccattc
      661 ccgcaaggtc tttcttcttc gtctctttac cgcctgctgc gaactgtgct gtagctcctg
      721 tctttgttgc ttttgatgac tgtccgcctg tattggagta aacctctgta tcaaatacca
      781 tgatgttgat atctttaccg cttgcaagaa catggtcaac accgccgtat ccgatatcgt
      841 aagcccatcc gtctcctccg aatacccact gggatttctt agcgaggaaa tctttatttt
      901 tcagaagttc cgctttctct gcgcggtcgc atccgcaagc ttcaagagcc gccacaagtt
      961 tatctgtagc tgttccgttt gtcgctccgc atgtatatgt gtcaaggtat tcctgtgcag
     1021 cagccttaac atcttcattt tccgcatctg cggcaagttt ctctacaaga cctttcagtc
     1081 tgtttctgat cgtattctgt gcaagaagca taccatatcc gaactcagcg ttgtcttcaa
     1141 acaatgagtt gctccatgcc ggtccttttc cttccggtgt taccgtatat ggtgtggacg
     1201 gagaagagtt gccccagata gaagaacatc ctgtagcatt cgcaatatac attctttctc
     1261 cgaaaagctg tgtgatcagt tttgcgtacg gtgtctctcc acatcccgca caagctcccg
     1321 agaactcaag aagcggctgt ttgaactggc ttccttttac tgtcgtctct ttgtatttag
     1381 cgacaacttc cggtttcacc ggaatctctc ttccgtaatc gaagaatgtc tgtttgcctg
     1441 cattctcttc catgttgccc ataacaagag ctttctcacc cttcttgccc gggcagacat
     1501 ttgcacaaga tccacatcct gtacagtcat aagcggatac tgtgatagcg aacttcatat
     1561 ccggcatacc gatcatcgga atagactgca ttccttccgg agcattagca gcttcttctt
     1621 ctgtcagagc taccggacgg ataacagcat gaggacatac atatgcacag cggttacact
     1681 ggatacagtt gtccggctgc cagatcggaa tgtctaccgc gataccacgt ttctcgtatg
     1741 cggaagatcc tgacggagtt gatccgtctg cataatctgt aaatgcagat accgggaggg
     1801 aatttccttc ctgagcattt acttttgtct gaatattttt aacgaaatcg attacatctt
     1861 ttcttccgtt ctcgtcaatg tgcggtactg caaggccttc gtctgccgca tctttccagc
     1921 tttccggaac ttcgatctct acgacctgtt ttgctcctgc atcgatcgca tcatagttca
     1981 tctgaacgat cttatcgcct tttctgccgt atgtagcttt cgctgcggct ttcatcaggc
     2041 tgatcgcttc ctcttccgga atgatctccg caagtttaaa gaacgcagac tggagcactg
     2101 tattgatacg tccgccaagt ccgatctctt taccgatctt aattccgtcg atcgtataga
     2161 acttaatgtt atggtttgcg atataagctt ttacctgtcc cggaagatgt ttctcaagac
     2221 cttccatatc ccacggacag ttcaaaagga atgttccgcc gtcaacaagc tcctgtacca
     2281 tgttatactt gtccacataa gacgggttat gacatgctac aaagtttgcc tgatggatca
     2341 ggtatgtcga tttgatcggt gattttccga aacgcaggtg agacattgta acaccgcctg
     2401 atttcttaga atcatagtca aaatatgcct gagcatacat atctgtatta tcaccgatga
     2461 tcttgataga gtttttgttt gctccgactg taccgtcagc tccgagaccc cagaacttac
     2521 agttgatcgt tccttccggt gttgtaacga gcggctcgtc tgctttcagg gaaagatttg
     2581 tcacatcgtc aacgataccg agagtgaatt ttgctttttc atcgttatga tatactgcaa
     2641 cgatctgcgc cggtgtcgta tctttagaac cgagtccgta gcgtcctgtg taaatcggaa
     2701 ctgcgtcgaa cttagatcct ttcaacgccg caacaacatc caggtaaagc ggctcaccga
     2761 gagctcccgg ctcttttgtt ctgtcaagaa cggaaatctt cttaacagaa tccggaatcg
     2821 catcgatcaa cgcctctgcg ctgaacggtc tgtaaagacg aacttttaca acgccgactt
     2881 tttctcctgc cgctgtcagg taatcgatcg tttcttcaat cgtatcacat acggaaccca
     2941 ttgcaacgat cacatgttcc gcatcttctg ctccgtagta attgaacagt ttgtagttcg
     3001 ttccgagctt ttcgtttact ttgtccatat agttctgaac gattcccgga agggcgtcat
     3061 aatacgggtt gcacgcttct cttgcctgga agaagatgtc aggattctga gcggaacctc
     3121 taaggcacgg atgattcgga ttcaatgcat gagcacggaa ctcatcgatc gcatccatgt
     3181 tcaccatatc tttcagatct tcataatccc atgtctcaat cttctgaatc tcatgagatg
     3241 tacggaatcc gtcaaagaag ttaataaacg gaagttttcc ttcaagcgct gcgcaatgag
     3301 caaccggtgt caggtccata acttcctgta cgcttgattc acaaagcata gccgctcctg
     3361 tctgacgaca tgcataaacg tcggaatgat ctccgaagat agacaaagca tgacttgcaa
     3421 gcgcacgggc agatacgtta aatacgcccg gaagctgctc gcctgccact ttatataaat
     3481 tcggaatcat caaaagtaat ccctgagatg ccgtaaatgt cgttgtcaga gctcctgctg
     3541 ccaaagatcc gtgaacagct cctgccgctc ctgcctctga ctgcatctct gtaacttcta
     3601 cagtcgttcc gaagatgttc tctctgcctt ctgtcgccca ttcgtcaacg tgctccggca
     3661 taacggatga cggtgtgatc gggtagattg ccgcgacttc tgtatacgca tacgatacgt
     3721 gagcggctgc ctgattgcca tccatagtct tcatttttct tgccattttt ttgctcctcc
     3781 ttaagtttta cattgaataa aataaatttc tcacatgtgt cctcattata tctccaaact
     3841 tgtcaaaagt ctatagaggg cagtttgttt tttgtttatt tttagcaaca gttcagccat
     3901 acagatgtac gggaagcaca aaacatgcct gcatgttttt gcaagcggac atctgtatgt
     3961 gctaagcgcc cggttatgag taaaacagtg gcatcagcca caataagcac gcagtgcggt
     4021 tttacgaatg gcgcgagctg aactgtttct atttttaagc acatgtgaca tgaactgcct
     4081 taactgttct tatgtttttt tcgcagataa agcattgctg cccccgtgcc gactacgatc
     4141 gcacctgcaa gaagctggga gacggcaatc ccgaccccgg gaagaagaag ctggtctgtt
     4201 cgtaatcctt cgatccagaa tcttcccagt ccgtatccga acagatacat caaaaacagc
     4261 tcgccctcat actttttatg cttacgataa gcaaatagga taatgagcag taccatacac
     4321 caaagcgatt catacaaaaa agtcggatgc acctgaatat aactgactcc gtctatcctc
     4381 tctatatgcc ggcgcatctg ctccgtcaca tcggaagagc gcactgcatc taatggcagg
     4441 cgcatcgcaa acagactgtc cgtatatccc ccaaacgctt ccctgttaaa aaagtttccc
     4501 catcttccaa gcatctgccc gtttaataac gccatagcga tcgtatcgaa aatctgagga
     4561 gcagataaat gctttacttt cgcaagcacc agaacgctga tcacagctgc gatcacccct
     4621 ccgtaaatcg caagtccgcc ttctctcaga ttaaaaatat cgagcaaatt atctttatac
     4681 atatcccacg aaaagattac ataataaagt cttgccccga caattcccgc aatgacgccg
     4741 ataatcccca tatcaagata attttccgga ttctgacctg tccgtttcgc ttcacgggca
     4801 gcaatccaaa aaccaagcaa aattgccgtt ccgataatga ttccgtaata cgctatctct
     4861 attccgaata aatcgatgct ctttccgaca tttttaagat aaattcccaa attaggaaaa
     4921 ctgatctctg tatgcataga tcattccgct ccttttcatt ttttatttga cagaacaaac
     4981 atatgccctg tatcccctct aaaaattttc tatttacaca aaggcaggtc tttcatgtaa
     5041 gtaaaaaacg gatacagacc ggcaaagaag tgtatctgca cggttccatg ccgtccgtat
     5101 ccatcgctgc atattcttat acgttgaatc taaacagcat aatatcgccg tcctgcacaa
     5161 catattcttt tccttccagc cttacaagac ctttttcttt cgctgccgca tgtgttccac
     5221 atgccataag gtcatcatag ctgacaactt ccgcacggat aaatccgcgt tcaaaatccg
     5281 tatggatctt tcctgccgcc tgcggcgctt tcgttcctct cttgatcgtc catgcacgga
     5341 cttccggttc tcccgccgtc aggtaactga tcagaccgag aagatgataa cttgcgcgga
     5401 tcaatttttc aagacccgac tctttcaatc ccagttcctc aaggaacatc gccttttcgt
     5461 catcatcaag ctgggaaatc tcttcttcta tctccgcaca tacaacgaac acttcacagt
     5521 cctcgcttgc cgcatactca cgaaccgcct ttactccttc attgttcgca ccatcgtccg
     5581 caaggtcgtc ttcagataca tttgccgcaa aaataacagg ctttgcagtc agaagattat
     5641 aacttgcaag ccactcctgc tcgtcctcgt catctgtcac aaagcttttc gccattttat
     5701 tgtcttccag atgcgctttc acacgattta aaagatccag ctcttttgca gccgccttgt
     5761 cattgcggga aagcttaacc gttttcgcaa tacgccgctc cagaatctcc agatccgaaa
     5821 agatcagctc aagattgatc gtctcaatgt ctctgatcgg atcgatgctt ccgtccacat
     5881 gaacgatatt tgtattttca aaacagcgga caacatgaac gatcgcgtcc acttccctta
     5941 tatttgcaag aaactggttg ccgagacctt cgccttttga cgcgcccttt accaaccccg
     6001 caatatcaac gaactcgatc gccgcaggaa taattttttt cgtatgatac atctctccga
     6061 gtacatcgag acgcttgtcc ggaactgtta cgactcctac gttcggatcg atcgtacaga
     6121 acggataatt ggcggattcc gcccctgctt ttgtcaatga attaaacaat gtacttttcc
     6181 ctacgttcgg caaaccgaca atacctagtt tcatttttat ctttacctgt ctaaaaaccc
     6241 tgcaattttg cgggttttcg cctttctcta tatttttaag tatcagagtc ttaatgtcgt
     6301 tctatataat actataataa ggctcatatg tctacatcac aactctaaag tattatctaa
     6361 aagtttcccg ggaaaagcca tatttccctt cactgactta aaacgacaac aaaaggcggt
     6421 atttaccgcc ttttgcttcc ggatcagctc tgcttattat tttgtatttg taatggtatt
     6481 tttagatctg cgatatttca gtattacaat gattgcagac aatgcaagaa ccattcctat
     6541 tcccgttcct ataaccgggg catgatctcc cgttttcagc gcatttgtct tactttcttg
     6601 attgtttaaa tcttccgtat cggcaccgcc gtgaccactt ccgttgtgat cgatattatt
     6661 atcgtgatta ttatctgtgt tatcgtcatc cgaagaatca atattttgcg atatcagttt
     6721 gtaagcagca actgttcctg acacttcgca ggatgcaagc agataagcat ttccgtctgc
     6781 gctttcagat gcggaaatca tgcacagtcc ttccggagat acgtcgcctg ctatatcttt
     6841 actaaaatct cgggagttaa tataatttgc gaaaagaatc ttgtcagggt ttgtaatgtc
     6901 ataagccata acgcctccga tcctctcaag accgataaac gcataagttt tctctcctac
     6961 cgttccgatt gttacgcttt ctgcttctac gccctttttc cctgaacggt catctatttc
     7021 tgcggagtca ttggaacaat taaagtactg cgggaaatat gccgctgttt ttgcttctaa
     7081 ctcgtttccg ctcgtataca cttctttcat tccggatcca tctacacaat aaacagtgaa
     7141 ggaacgtcct ccgaacaggt aatcatattc tgaatttaaa ccttcataat cgttctgatc
     7201 gaagaataca acctttccgg ttagtccact gttttcagcc gtaattttcc cggaaggtga
     7261 tgtttgccct ttcccaaaat tacattctgc ttcattaaga tattcgttcc actctctgga
     7321 atccccctca tttgcagtta caatataagt tttgccgttc gactcaaaca gagaaattcc
     7381 gtccggcata cggattccgc gaagcgacgg ataagatacc ggcctgtatg ccgcatcttt
     7441 tttatcaata tcgatcggat atttttcgta atcttcatat cctgcggaac agatattttc
     7501 tatagaaagt gtttgcagat ctacgatggc aatggcgttt gcttcctgaa gtgtaacata
     7561 ggctgtttta tcatttgctg caatatactc cggttctaaa tcaacagacg gagccgttcc
     7621 ttttttcaaa atcactccat tttgtacaag ctccgctctt ttcgcttcac tgtcaaaaac
     7681 ggtaaagtca agatgtgata cattctgatt tgtcagatca attacagtga ccgttccctt
     7741 agggtcaata cttcccgctt catagccgtt tcttggttcg ccttcatctg ccgaaagaat
     7801 ttttttacta tcgggtgtaa atgtcaccat atccggctgt acacctgcct cataaatctg
     7861 atcaaatgac aatgtcccat ctgcgccgca aaggaatact gcgaggcatc cggcaaccga
     7921 ataatcagat gactggattg ccactgcaag cttttctcca tttggagaaa cggcaacaga
     7981 agtcatatca ccgtaggaga aacctttgta attgttctca acgatttctt tgatatttat
     8041 attctttcca tctaacaaag cgatcttatc aactgcattt ttatttttca tcggaattgc
     8101 cgttaagttc ccggttacac cattcacagc atacgcccag cctgtaactg tattgtaatc
     8161 tataatttcc ataacgccgc cgtccgggtt ttgcatgccg gaattatatt gagcagtctg
     8221 tgtcatggca atagaagact ttttattctc taatccgttt ttcagctctc cgccgttcgc
     8281 atttttggta acactgaatg aatcaattaa tttactttcc ggggcattgc tgtctcccga
     8341 aaggttatat acgttgacag agattttatt atccgccacg tcaaagacag cgtaacttgg
     8401 actgtactcc tggttccatt cctgatttcc aattggaaga tttcccgcca aatatgcctc
     8461 agaaccggtt tgtccgtttg ccagcaccgg ataatctgca ttattggtct tgtctacttt
     8521 ctcaaaagga gtatagtatt gcatgtcaga acagcaatta cccgtcagat aaatcactcc
     8581 gtcaggatca tggaaatcat ccagtctttc tgcatttctc tgaccgtttt tcagcacata
     8641 gcttcgagaa tacacatgat cgtgaccgcc taatacaaag tcaacgtcaa attcatccat
     8701 cacctgctca aatcctgcat ctacatagtt ctcaatatcc gaatcggcag catgctttgc
     8761 cactgtctgc gtagatttat gatgtgtaac aactaaccat tgatactgac ctgaatattc
     8821 tgccgtcgcc gcatttaaag ttgttctgaa gttagaaacg atggcttccg cttcggaatt
     8881 gtcctttgaa gcgcttggaa catttacatt tcccgcattt tcattatcgt ttccgcccgg
     8941 ataagctcct gtgtttaatg ttacgaagag aacgttatta tataaatagt aatagtttcc
     9001 ttttgtctcc atttctcttc gttccacaaa atcatattgc ccgtctttat tcggtgtaac
     9061 accatttgac tctgcagatt gtgcgatttc atccgcagtt gcctgaatat agttgccatg
     9121 gctttgactc gttccactgt tctgtccgcg gaatgttgtc tttacctgat taagagacgc
     9181 tgcgtttcca tctacactca tttcattcgg aagattaaaa tgatcatcaa acatgtaatg
     9241 acgatcatga tttcctactg ccggggcata cagaagagat gtcatttctt ccggagcaaa
     9301 aaatgccgca tactcactgg attttcccca gctttgatcc tccacctggt ctcccgcgga
     9361 aacaatgagg ttcacatctt cctctttcat cttgtccagc ataactgccc atccggtctg
     9421 attttttccg tcagaaggat tccatccccc gttatttgtt tctccgtttt ctttaatctg
     9481 gggatcgctt gtaaaaccaa atcgaaattc acctgcattt ggtgtttcat aagaatactt
     9541 ctttgaccaa gtatttccat tatcatatga aatctgataa ctatattctg tgccttttga
     9601 aagtcctgat acagtagcct gacatactat tttcccggta tctgtatatt ttccttcaac
     9661 tacttttgta ggagtatgaa gagctcttac agatgcttct actgtcttat cccccatacg
     9721 gacaatcgca ttttgagtac cttccggggc ataccagttc aggtttaccg aatcgtctct
     9781 ttcacctggt tgtaaagtaa ggtattcgat tgaaactgca ttgttttcct gtgctgccac
     9841 ctcctctgct cccgctgcaa agacatcact tactgtactt tgagcgcata cgatcgaaac
     9901 agctgctaaa agcgccattg tttttctccg ttttactctc atatcgtttc ctccgttaac
     9961 ccattgaatt caagtaacga taggattgta atggtctttt gttaaggtat tttgttgcat
    10021 atgt
//
LOCUS       DBMDXF010000055         9678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1928_length_9678_cov_1.836483, whole genome shotgun sequence.
ACCESSION   DBMDXF010000055 DBMDXF010000000
VERSION     DBMDXF010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1928_length_9678_cov_1.836483"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="ACZPD4_03635"
     CDS             complement(<1..893)
                     /locus_tag="ACZPD4_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HIR1378198.1"
                     /translation="MGQGSIIGYEINEKTCQISFYNEKDLEPDTLEVDTDNYQIPLII
                     GKLRDTWAYGKEAKRLVTIKDGFTATRLLSKSLAGDKIEFGEETYDAVWLLSQFIQMS
                     LQSFPEIDGIVFTVPSLSEEMAQMLRGIAVRMNIDKRNIFIQDYKESFCNYLFYQPKE
                     LWQYDAALFCCDRNEIKAYMLRRLQPGLGGGKTTFVTVDEVASAHMKELAMVYPVLNE
                     DKAKEADAMFSKFIESVFDKRIVSSVFLTGEGFENDWYPKSLRVLCNGRRAFIGNNLY
                     SKGACYTAYRKLYMHIENPVYL"
     gene            complement(921..4220)
                     /locus_tag="ACZPD4_03640"
     CDS             complement(921..4220)
                     /locus_tag="ACZPD4_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HIR1378199.1"
                     /translation="MKNKIKRFAKGDFHIPQPEIIFPETHIVMRVGEGEKYRGSFSLK
                     NQGEGTIRGLVYPSSYRVQCDEQGFDGNPVNIYYTYDGSGLMPGHVEHGKFTIVCNGG
                     EYDVAFTAIIEKPYVMTSYGKIQSLDDFKKLSFRDAAEAVKLFRSRDFYEILKYEDKR
                     IQALYENMRKWELDQQALEEFLVGCKQKEKIFLTLEEESRAFMSLSEARKETFTIRKN
                     TWGYLEIDVHTEGEFLSVEHTRVTTEEFIGNSYRLEYFLNVEALHPGSNFGRIILESP
                     YETLTYEVVVEKDISRDEDYRANDREFAGIVKNYLKYESGKLELNEWVEEAIRRISHL
                     REVDDRNEFYLLAHAHICLIGKRLEEAKWLLESYNYNRFAIGKDVELSSYYLYLTTLL
                     SNDTIGQRKVAEELSKSFMKHPDSWKILCMLVEVDSEYKIYSERLRALEKQFYDEKSH
                     SVWFYLQAFKCYREKSSSLKKLGMFEVRVLLFAVKHKLMTRELALYTANLASQMKVFD
                     KQLYAVLVGSYKMYKESMILTAICTLLIKGNCVESCYFQWYEKAVEAELKIAQLYEYY
                     MASVVPADFHKALPRSVYLYFMHGNSLDYHKCAFLYSNLITYEDESSEIYAHYRDEME
                     AFAWNQLDRRNVDEQLRIIYKRFVVEASMNPERVKALYDVCHAYRITTKVPNMKFIHV
                     IADDGTITQKSPYTENGARVFLYAKTDRLVWESKDGRHYTDSIPYESQRLFYELRYMD
                     MCRKYINGLRRTREEEEVQELTTEIVRENGVENYEEDELLGLCSKTIRENNYENDDFL
                     TYVCFELFKKGQYDKVILTYLASYYCGATSDMKMLWREARDYEVHTHKLAERILTQML
                     FSEELFQEAQVFEQYYAEGAYFRLQQAYLVYMSREYVVEERKISRSVIDIICREYEKG
                     EDTIDICKVAVLKYYSTREYSPQTRKTLKKFLQELCGKQIYFPFFLSYEKDWLIELQL
                     WDKTLIEYKGQKGSRVMLYYSLQKGGEESSDYSTEVLTPMYENIYVKKFVLFANEKLK
                     YYFKETIDGNSYRSDKELCVRETVQGEPGRYGRLNDILIEKNESERKKKIQAYAREDA
                     AAAQIFTKEQA"
     gene            complement(4429..4878)
                     /locus_tag="ACZPD4_03645"
     CDS             complement(4429..4878)
                     /locus_tag="ACZPD4_03645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1378200.1"
                     /translation="MIRKMRSEDKKTYMEMAREFYHSDAVLHPVPDTYFERTADEALR
                     SDVYAEIFLFECENEAAGYGLIAKTYSQEAGGMVWWIEEVYVREAFRSKGIGREFFAY
                     LDKVKGSAVTRMRLEVEEDNTRAVALYKKLGYKPLEYAQMIKDAPLK"
     gene            complement(4880..6799)
                     /locus_tag="ACZPD4_03650"
     CDS             complement(4880..6799)
                     /locus_tag="ACZPD4_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1378201.1"
                     /translation="MGKKSCFFVYKSGDADVEKKNPEINFVKALSAVCAVLLWRNAFL
                     SVFDLEALANQIGRKEIIFAEIAVSFAVFILLIAAFKVRRLRVIAYLVIFAPFISAAC
                     ARKFPPPWASWMLVFAAVLYGTCCAMEGCKAKQVFRSLVLISAVFGIAAGISVFAGKQ
                     IDQIRVDENGFYQETRQKIRENVIGKAEELAEKIKNKKDGKKLDEEKTDAESKHDRED
                     IEEKNPFRPENDPTQQNGKNDNMTSGGKMDDLKAISSFKPSSEVSTCVVLEKKKTYLY
                     DRWGTTYEDSAWTNEKLEEVVYSSKYYFEEEKEELFLQYPSELTRMQKMCEGWDKSSF
                     SAVKNQIDEALSNIVYDTNPGKTPAKWDFAEYFLFENKKGFCVHFATTAALLYRMCGY
                     QSIYVEGLVVPASAFKEKENGTYEAQVDGTMGHAWCEVYDEKTGEWITMEHTPASSRN
                     EMQGADAAKQKKENSFKSNQVFRLIVCVVFVAGAAFGGVFIQAVVRGKRHRRKIGTTR
                     GGIGILTMYDDVVKVARLTEKPKKQIFGNRHSDTDDYSEVWLKHLKKQYPQISEEEWN
                     SFYEEVRRILFYYPTKDRRIWKESYKLYRKFYAEAKRRMNPGMRLLLKYIYCIETPFC
                     EKGASFMKKKKKRRK"
     gene            complement(6768..7820)
                     /locus_tag="ACZPD4_03655"
     CDS             complement(6768..7820)
                     /locus_tag="ACZPD4_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR1378202.1"
                     /translation="MVKRIIGYLAVLILTIYLFFMYNDTVISGLLIFVIMYPLLSFWF
                     LMIQRGKVGTDMTRIPSMGEEGKTIKAGITVKNQSAWNMRYEAVILVGNKIEEKKKQK
                     RIRGVIQAENVRTFWYELPGNICGSMEITLNEIRIYDPLCIFYIRRREAETVFVKIMP
                     EFELMPVEITRKTREFQTDAEEYSCEKKGDDPSEIYQVREYRREDSLKDIHWKLTAAK
                     EELVAKERAFPLGCAVLIWFDIREKECTANGFSKMLKTAASLSITLVEEKCIHLAAWY
                     EEDTEQIVTVKVKDEESCCQMVWELIDIKPCGNTEKRDSYMRERFKGAEFSSIVTIDG
                     QGQIKKDGKEELFLRL"
     gene            complement(7831..8784)
                     /locus_tag="ACZPD4_03660"
     CDS             complement(7831..8784)
                     /locus_tag="ACZPD4_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1378203.1"
                     /translation="MEENRCRARSVIGEVEKSVVGKTDILRKVMTAILAGGHVLIEDI
                     PGTGKTTLALSFSHAMGLKVNRVQFTPDVLPADLSGFTIYDKASGTFVYQPGAAICNL
                     LLADEINRTSPKTQSALLEVMEEHQVTVDGKTHRTGEPFIVIATENPVGSAGTQLLPE
                     SQLDRFMICVSMGYPTAEEEIEILKREQSIEKTRERIDPVVNAEELIEMQREVKEVFV
                     HDRIFGYIVELAKATRENEWTELGLSPRGTVALTAMAKACAWLNGREYVVPSDVQEIF
                     PCVTEHRIILNMKAKVGHIKKEELIRRVLESVPTPTMRQKY"
     gene            complement(8989..>9678)
                     /locus_tag="ACZPD4_03665"
     CDS             complement(8989..>9678)
                     /locus_tag="ACZPD4_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1378204.1"
                     /translation="KYQIKAACVCFGAGFDKELAKDPELFSRELERAVEIAYELGAKV
                     VNHYCYHVAMGPEISFSELEKYYGRAIRKAKHRKIYLALENEAHDITQSPEGMKTIVE
                     HMNSEYFKTNFDATNYYQAGYEGFPYAYEVLKEYIVHVHIKNGCFYHPEFGHEKQCRG
                     GKMTGMFAGNDIYYPYASDGAVNIDGLLRRLEKDGYTGFCTMEPHTTPEKCLDFYREE
                     TAYLRERGIKE"
BASE COUNT         2579 a         2525 c         1446 g         3128 t
ORIGIN      
        1 agatataccg gattttcaat atgcatatac aactttctat acgctgtata acaagcgccc
       61 ttactgtaga gattattacc gataaatgct cgccgcccgt tgcacagcac tcgcaaagat
      121 ttcggatacc agtcgttttc aaaaccttct cctgtcaaaa atacagagga aacaatccgt
      181 ttgtcaaata cgctctctat aaatttgctg aacattgcat ccgcttcttt tgccttatct
      241 tcattcaaga caggatatac catcgcaagt tccttcatgt gagcgcttgc cacttcatct
      301 actgtaacaa acgtcgtttt tccgcctccc agccccggct gaagcctcct aagcatatac
      361 gctttgatct catttctgtc acagcaaaac agcgctgcgt catactgcca caattccttc
      421 ggctgataaa acaggtaatt gcaaaaactc tccttgtaat cctggataaa aatattacgt
      481 ttatcaatat tcatccgtac ggcgattcct ctgagcatct gcgccatttc ttccgaaaga
      541 gacggaactg taaaaacaat accgtcgatc tccggaaatg actgaagcga catctgaata
      601 aattgagata aaagccacac tgcgtcgtat gtttcttctc caaactcgat cttatcccca
      661 gcaagacttt tgcttagaag tctcgttgcg gtaaatccgt ctttgatcgt aacaagtctt
      721 ttcgcttctt ttccatacgc ccacgtgtct cgcagttttc ctatgataag cggaatctga
      781 taattgtccg tatccacttc cagcgtatcc ggctccaagt ctttttcgtt ataaaaactg
      841 atctgacatg ttttttcatt tatctcatat ccgatgatac ttccctgtcc catgaaaaaa
      901 caacctccta ttgtttaatc ttacgcctgt tcttttgtaa aaatctgcgc tgcagccgca
      961 tcttctcttg catatgcctg aatttttttc ttgcgctcac tttcgttttt ctcgatcagg
     1021 atatcattca gtcttccata gcgccccggt tctccctgaa cagtttcccg cacacaaagt
     1081 tccttatcac ttctgtaaga gtttccgtcg atcgtctctt taaaataata ttttaacttt
     1141 tcatttgcaa aaagaacaaa ctttttcaca taaatatttt catacatcgg agtaagcact
     1201 tccgtcgaat aatcggacga ttcttctcct cctttttgca ggctgtaata aagcattacc
     1261 ctgcttccct tctgaccttt atactcgatc agtgttttat cccaaagctg cagttcgatc
     1321 agccagtctt tttcatatga aagaaagaac ggaaaataaa tctgctttcc acacaattcc
     1381 tgaaggaatt tctttaacgt ctttcttgtc tgcggactgt actctctcgt cgaatagtat
     1441 tttaataccg ctactttgca tatatcgatc gtatcctcgc ccttttcata ttcacggcag
     1501 atgatatcga tcacactcct gctgattttt ctttcttcca ccacatattc tctcgacata
     1561 tacacaagat acgcctgctg aagcctgaaa tatgcacctt ccgcataata ctgctcaaac
     1621 acctgcgctt cctgaaataa ttcttccgaa aagagcatct gtgtcaggat cctctctgcc
     1681 agcttatgcg tatgtacttc ataatcccgc gcctctctcc aaagcatctt catatcagat
     1741 gtcgcgccgc agtaatagct tgccaaatac gtcaggatca ccttgtcata ttgacctttt
     1801 ttaaacaatt caaagcagac atatgtaaga aaatcatcat tctcataatt attctctctg
     1861 atcgttttgc tgcaaagtcc caaaagttca tcttcttcgt aattttcaac tccgttttct
     1921 ctgacgatct ctgtcgtcag ttcctgaact tcttcttcct cccgcgttct tctcaggccg
     1981 ttaatatatt tcctgcacat atccatatag cgaagctcat aaaaaagtct ctgactctca
     2041 tatgggatag aatctgtata atgccttccg tcttttgact cccacacaag ccgatcggtt
     2101 ttcgcataca agaatacacg cgctccgttt tccgtatacg gtgatttctg agtaatcgtg
     2161 ccgtcatctg caataacatg aataaatttc atattcggca ctttcgtcgt aatccggtat
     2221 gcatgacata catcataaag cgcttttacc ctttcaggat tcatagatgc ctctactaca
     2281 aaccgtttat aaatgatacg aagctgttcg tcaacatttc tccggtcaag ctgattccat
     2341 gcaaacgctt ccatctcgtc tctgtaatga gcgtaaatct cgctgctttc atcttcatat
     2401 gtgatcaagt ttgaatataa aaatgcgcat ttatggtaat ccaatgaatt tccgtgcata
     2461 aaatacaaat ataccgatct cggaagcgct ttgtgaaaat ccgccggaac aaccgaagcc
     2521 atataatatt cataaagctg agcaatctta agctctgctt ccaccgcttt ttcataccac
     2581 tgaaaataac agctttccac acagtttcct ttgatcagaa gagtacagat cgccgtcagt
     2641 atcatcgatt ccttatacat cttataagaa ccgacaagaa ccgcatataa ctgtttgtca
     2701 aaaactttca tctgacttgc aagatttgca gtgtaaagcg ccagttctct tgtcatcagt
     2761 ttatgtttga ctgcaaacag aagaacccga acttcaaaca ttccgagctt tttcagagaa
     2821 gaactctttt ccctgtaaca tttaaacgcc tgtaaataga accagacgct atgcgacttt
     2881 tcatcataaa actgtttttc caaagcccga agacgttcac tgtatatttt atactccgaa
     2941 tccacttcca caagcataca caaaatcttc caactatccg gatgcttcat aaatgacttg
     3001 gaaagctctt ccgcaacctt tcgctgcccg atcgtgtcat ttgacaaaag cgttgtcaaa
     3061 tacaaataat atgaactaag ttccacatcc ttaccgatgg caaagcgatt ataattatat
     3121 gattcgagaa gccatttcgc ctcttccaaa cgttttccga tcagacagat atgtgcatgc
     3181 gcaagaagat agaattcatt tctgtcatca acttccctca gatggctgat ccgacggatt
     3241 gcttcctcga cccattcgtt cagttccagt tttccacttt catattttaa atagttttta
     3301 acaattcccg caaactctct gtcgtttgca cgataatcct catctctgct tatatctttt
     3361 tctacgacaa cctcataggt aagagtctcg tacggagatt ctaatatgat cctgccgaaa
     3421 ttacttcccg gatgaagtgc ttccacattc agaaaatatt ccagccggta tgaatttccg
     3481 ataaactctt ccgtcgttac acgggtatgt tcaacagaga ggaattcccc ttctgtatgt
     3541 acgtcgattt ctaaatatcc ccacgtattt tttctgatag taaacgtctc tttccgcgcc
     3601 tctgaaagcg acataaacgc ccggctttcc tcctcaagtg tcaggaatat cttctctttt
     3661 tgcttacatc ctacgagaaa ttcttccaaa gcctgctgat ccagttccca tttccgcata
     3721 ttttcataaa gcgcctggat ccgcttatct tcgtatttca gtatttcgta aaaatctctg
     3781 gagcgaaaca gctttactgc ctcagccgca tcccgaaaag acagcttctt aaaatcgtca
     3841 agactttgta tcttcccata gctcgtcata acatacggtt tctcgataat cgcggtaaat
     3901 gcgacatcat actcgccgcc gttgcaaacg atcgtaaact tcccatgttc gacatgcccc
     3961 ggcatcaacc cgctgccgtc atatgtataa tatatattga caggattccc gtcgaaacct
     4021 tgttcgtcac actgcacacg ataagaagag ggatagacaa gacctctgat cgttccttcc
     4081 ccctgatttt tcaatgaaaa acttcctctg tatttttccc cttctccgac tctcattaca
     4141 atatgtgttt ccggaaatat aatttccggc tgcggaatat gaaagtcgcc tttggcaaat
     4201 cgtttgatct tattcttcaa atctgtcacc tactcatcat ttttgcagca gtccgggcat
     4261 gaacgtatcg cggactgctg tcttttgccc tcggacaaaa gtacgctact atgaattata
     4321 acattgttct gtctcttctt gcaatggggt attaaaagaa tttcagagat attgtataca
     4381 agtatacgat atctctgttt ggctaaaatg ctctgttttc aaacgatttt acttcaaggg
     4441 agcatctttg atcatctgcg catattccaa aggcttgtat ccaagttttt tatacagagc
     4501 tacggctctt gtattatctt cttccacttc aagcctcatc cttgtcaccg ccgagccttt
     4561 caccttatca agatatgcaa aaaattccct tccgatccct ttggaacgaa acgcttcccg
     4621 cacatacact tcttcaatcc accaaaccat tcctcccgct tcctgagaat aagttttcgc
     4681 gatcagcccg tatcccgccg cttcgttttc acattcaaaa agaaatatct cggcatagac
     4741 atcagagcga agcgcctcat ctgcagtccg ttcaaaatag gtgtccggaa ccggatgtaa
     4801 tacggcatcc gaatgataaa actctctcgc catctccata taagtctttt tgtcttctga
     4861 tctcattttt cttatcattt tactttctcc ttttcttttt ctttttcata aaacttgcac
     4921 ctttttcaca aaaaggcgtt tcaatgcaat aaatatattt tagaagcagc cgcattccgg
     4981 gattcatccg cctttttgcc tcagcataaa acttccgata caatttatag gattctttcc
     5041 atattctccg gtcttttgtc ggataataaa acaaaatgcg ccgcacttct tcataaaaac
     5101 tattccactc ttcttccgaa atctgaggat actgtttttt cagatgtttc aaccaaactt
     5161 ccgaataatc atctgtatcc gaatgcctgt ttccgaaaat ctgcttcttt ggtttttccg
     5221 tcagtcttgc aactttaact acatcgtcat acattgtaag aataccgatt ccgccccgag
     5281 tcgtcccaat ctttctcctg tgacgttttc ctctgactac agcctgaata aaaacgcctc
     5341 caaacgcagc ccccgccaca aaaacaacac acacaatcaa ccgaaaaacc tgattgcttt
     5401 taaaactgtt ttctttcttt tgttttgccg catccgctcc ctgcatctcg tttcttgaag
     5461 atgccggcgt atgttccatt gtgatccact ctcctgtttt ctcatcatat acttcacacc
     5521 atgcatgtcc cattgttccg tcaacctgcg cctcataagt tccgttttct ttttctttaa
     5581 acgcagaagc cggaactacc aaaccttcca cataaatcga ctggtagccg cacatcctgt
     5641 aaagtaaagc tgctgttgtg gcaaaatgta cacaaaatcc ctttttattt tcaaacagaa
     5701 aatactctgc aaaatcccat tttgcaggcg ttttcccagg attcgtatca taaactatat
     5761 tcgacaaagc ttcgtcaatc tgatttttca cagcagaaaa cgaactctta tcccatcctt
     5821 cgcacatttt ttgcatacgg gtaagttccg aaggatactg taaaaatagc tcctcttttt
     5881 cttcttcaaa ataatacttt gaagaataca ctacctcttc tagtttttca tttgtccacg
     5941 cactgtcctc ataagtcgtc ccccacctat cataaaggta tgttttcttt ttctcaagca
     6001 caacacatgt agacacttcc gaagacggct taaaagaaga aatagctttg agatcgtcca
     6061 tttttccgcc cgacgtcata ttgtcatttt ttccgttttg ttgtgtcgga tcattttccg
     6121 gccggaaagg atttttttct tctatatctt ctctgtcatg cttgctttca gcatctgttt
     6181 tttcttcatc taactttttg ccgtcttttt tattttttat tttttcagcc agttcttctg
     6241 cttttccgat tacgttctct ctgattttct gtctcgtttc ctgataaaat ccattctcgt
     6301 ctactctgat ctgatcgatc tgttttcccg caaaaacaga aattcccgca gcaatcccaa
     6361 agaccgccga aatcaaaaca agtgaacgaa aaacctgttt agctttgcat ccttccattg
     6421 cacagcacgt tccatacaat accgcggcaa aaaccaacat ccacgacgcc catggcggtg
     6481 gaaattttcg cgcgcatgca gcagatataa acggtgcaaa aataaccaaa taagcaatta
     6541 cgcgcagcct ccttactttg aatgccgcaa tcaacaaaat aaataccgca aaactcacgg
     6601 caatctctgc aaaaatgatt tcttttcgtc ctatttgatt tgccagcgct tccaaatcaa
     6661 atacagataa aaatgcattc cgccataata agactgcaca aacagcacta agcgctttca
     6721 caaaatttat ttccggattt ttcttttcca catcggcatc cccactttta taaacgaaga
     6781 aacaactctt ctttcccatc ttttttaatc tgcccctgtc catcgatcgt aactatactg
     6841 ctaaactctg cccctttaaa acgctccctc atataggaat ctcttttctc tgtatttccg
     6901 cacggcttta tatcgatcaa ttcccatacc atctgacagc agctttcttc atctttcact
     6961 ttcacagtca caatctgttc tgtatcctct tcataccacg cagccagatg aatacatttt
     7021 tcttcaacaa gagtgatcga taatgatgct gctgtcttta gcattttaga aaaaccgttc
     7081 gcagtacatt ctttttctct gatgtcaaac cagatcagta cggcacatcc taaaggaaac
     7141 gccctctctt ttgctacaag ctcttctttc gcggcagtca gtttccaatg aatatctttt
     7201 aaagaatctt ctcttcgata ctctctcacc tgataaattt cagaaggatc atctcccttt
     7261 ttctcacagg aatattcttc cgcatctgtt tgaaattctc tcgtctttct tgtgatctca
     7321 acaggcatca gttcaaattc aggcatgatc ttcacaaata ctgtttcagc ctctcttctc
     7381 ctgatataaa aaatacaaag gggatcataa attcttattt cattcaacgt aatttccata
     7441 ctgccacaaa tattccccgg cagctcatac caaaatgttc tgacattttc cgcctgtatc
     7501 actccccgta tccgcttctg tttctttttt tcttcaatct tgtttcccac aaggatcaca
     7561 gcttcatacc gcatattcca tgcggattga ttttttacgg taatcccggc ttttatcgtc
     7621 tttccttctt cacccatcga aggaatacgc gtcatatctg ttccgacttt tcctcgctgg
     7681 atcatcaaaa accaaaatga taaaagcgga tacattataa caaatataag aagtcctgag
     7741 attacagtat cgttatacat aaaaaataaa tatatcgtaa gtataagaac tgccagatat
     7801 ccgattattc tttttaccat ctgttcatcc tcaatatttc tgtctcattg tcggagtcgg
     7861 aacactctcc agaacccgcc ggatcagttc ttcttttttt atatggccta ccttcgcttt
     7921 catattcaga atgatccgat gctctgtgac acacggaaat atttcctgta catcggaagg
     7981 aacgacatat tcccgtccat tcagccacgc acatgctttc gccatagctg tcaacgcgac
     8041 agttccgcga ggacttaaac ctaactctgt ccattcattt tcccttgttg cttttgcaag
     8101 ttccacaata taaccgaaaa tccggtcatg tacaaaaacc tccttaactt ctctttgcat
     8161 ttcgatcaat tcctctgcat tgacaaccgg atcgattcgt tctctcgttt tttctattga
     8221 ctgttcccgt ttcaaaatct caatttcctc ttctgccgtc ggatagccca tactcacaca
     8281 gatcataaaa cgatcaagct gtgactccgg cagaagctga gttccggcgg aacctaccgg
     8341 attttcagtc gcgatcacaa taaacggctc tcctgtcctg tgagtttttc catcaaccgt
     8401 tacctgatgc tcttccatta cttccaaaag cgcggactgc gtctttggcg acgttctgtt
     8461 tatctcatct gccaaaagaa gattgcatat agctgcaccc ggctgataca caaatgtccc
     8521 tgatgcttta tcatatatcg taaatccact cagatctgcc ggaagtacgt ccggcgtaaa
     8581 ctgcacccga ttcaccttaa gtcccattgc atgcgagaac gaaagcgcca gcgtcgtctt
     8641 tcccgttccc ggaatgtctt cgatcagaac atgacctccg gcaagaattg ctgtcataac
     8701 ttttcttaaa atatctgttt tcccaacgac tgatttctcc acttccccta taactgaccg
     8761 cgctcgacat ctgttttctt ccataagtta ttctccccga tcttattcgc ttttgcctct
     8821 atatttgcac acgtttatta tcctgtttca tctacttata atctactcta tgtacaatat
     8881 caaaatcaac cgttatacag aattttccgg taaaaatgtg acagttcacc tgcccattca
     8941 taaaaatgcg gcgaaaatga ccgctgtcat cttcgccgta tatatcgttt attctttgat
     9001 tcctctttct cttaaataag ccgtctcttc tctgtagaag tccagacact tttcaggagt
     9061 cgtatgaggt tccatcgtac aaaaacctgt atatccgtct ttttcaagcc tccgcaaaag
     9121 tccgtctatg ttcaccgctc cgtcagatgc atatggataa tagatatcat ttcccgcaaa
     9181 catccccgtc attttccctc ctctgcactg cttttcgtgt ccgaattcag gatgataaaa
     9241 acaaccgttt tttatatgta catgaacaat atactccttc aaaacttcat atgcatacgg
     9301 aaaaccttca tatcccgcct gataatagtt ggtcgcatca aaatttgttt taaaatattc
     9361 cgaattcata tgctctacaa tcgttttcat cccttcggga ctttgagtaa tatcatgagc
     9421 ctcattttcc aaagccagat agattttcct atgcttcgct ttcctgatcg cccgcccata
     9481 atacttttcc aattcagaaa aagaaatttc cggacccatt gcgacatgat aacaataatg
     9541 atttacaact tttgctccca gctcataagc tatctcaaca gctcgttcta attctctcga
     9601 aaaaagttcc ggatctttcg ccaattcttt atcaaatcct gctccaaagc atacacacgc
     9661 tgcttttatt tgatattt
//
LOCUS       DBMDXF010000056         9678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1929_length_9678_cov_1.476872, whole genome shotgun sequence.
ACCESSION   DBMDXF010000056 DBMDXF010000000
VERSION     DBMDXF010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_9678_cov_1.476872"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="ACZPD4_03670"
     CDS             <1..1011
                     /locus_tag="ACZPD4_03670"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1378205.1"
                     /translation="EGNQASLYVIRNENGMEAAVTDLGATLVSLKVKNKEGSMADVVL
                     GYENAAGYEAGTCFFGTIVGRNANRIGGAQFELNGKTYHLTGNDNGNNLHSGMDFYNI
                     RIWETEEVTETSVRFKLHSPDGDQGYPGTADIYVTYSLDKDDKLDIFYEALSDKDTIL
                     NMTNHSYFNLDGHNSGSVLQHTLTLEADYFTPGDAQSIPTGEILPVENTPMDFREGRK
                     IGERIEEEYEPLRFGNGYDHNWVLKNKGNFEKVAELMAEESGIVMETYTDRPGMQIYT
                     ANFVENEVGKEGVVYGKRSAVCLETQCYPDAVHHENFPSPVYRAGEKYTAHTAFRFLI
                     KK"
     gene            1336..2610
                     /locus_tag="ACZPD4_03675"
     CDS             1336..2610
                     /locus_tag="ACZPD4_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1378206.1"
                     /translation="MKRKIALITMAAVMALSVTACGGGSDSGKSASNNSSGSEVANKD
                     KPLVWFNRQPSNSSTGELDMTALNFNKDTYYVGFDANQGAELQGTMVKDYIEKNIDKI
                     DRNGDGVIGYVLAIGDIGHNDSIARTRGVRKALGTGVEKDGDIDSAPIGTNADGTATA
                     VKDGTIEAGGKKYVVRELASQEMKNSAGATWDAATAGNSIGTWSSSFGENIDVVVSNN
                     DGMGMSMFNAWSKDNKVPTFGYDANNDAVAAIAEGYGGTISQHADVQAYLTLRVIRNA
                     LDGVDIDTGIGTEDEAGNVLSEDVYTYNEEQRSYYALNVAVTADNYQEFTDSTKVYEP
                     VSKQLDEKEHAKKKVWLNIYNASDNFLSSTYQPLLKNYDDLLNLDVEYIGGDGQTESN
                     ITNRLGNPSQYDAFAINMVKTDNAASYTSLLQ"
     gene            2849..4357
                     /locus_tag="ACZPD4_03680"
     CDS             2849..4357
                     /locus_tag="ACZPD4_03680"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1378207.1"
                     /translation="MADKKDNIVLSIRDMSKSFGRNRVLTRINLDVKAGTVMGLMGEN
                     GAGKSTMMKCLFGTYQKDEGNIFLDGKEVSFSGPREALENGIAMVHQELNQCLERNVV
                     DNLFLGRYPVNAVGVIDEGRMKKEASELFRKLGMTVNLSQPMRNMSVSQRQMCEIAKA
                     ISYNSKVIVLDEPTSSLTVQEVEKLFEMMRMLKEQGIALIYISHKMDEIFEICDEISV
                     LRDGNLVMTKSTEDTDMNELITAMVGRSLENRFPEVDNTPGDVVLSVQHLSTKFEPYL
                     KDVSFDVRKGEIFGLYGLVGAGRTELMETIFGVRTRAAGRVYFNNHLMNFESAKDAME
                     HGFAMITEERKANGLFLKGDLTFNTTIANLKQYKSGIALSDSKMEKATAEEIKTMRTK
                     CMGPNDMISNLSGGNQQKVIFGKWLEREPQVFMMDEPTRGIDVGAKYEIYELIINMAK
                     QGRTIIVVSSEMPEILGITNRIGVMSNGYLSGIVNTKETNQEELLRLSAKYL"
     gene            4378..6033
                     /locus_tag="ACZPD4_03685"
     CDS             4378..6033
                     /locus_tag="ACZPD4_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1378208.1"
                     /translation="MANGNSKVLTAEQEMQIRRPIEEYVGAIQKQIDGLRVDGTDKVL
                     SLQNTMDGVKRDRTLTKGEKEDRLTRMRRELQQAKAVESKNKDRISKLIADAEAYLKE
                     HFDKEYYVPVKESCAQEKVLAKEKYQKRVEELKKEHQQILSKLSEHQEIKDEKYVYKN
                     RLFDAKMELQKDYQTIKDRRHAAYSYKYHLIDLLRMSKFTFLETRAQKWENYKYTFSK
                     RNFFLQNGLYIAILLIFIVLCIITPMIKGAPLLTYNNVLNVLQQASPKMFLALGVAGL
                     ILLAGTDLSIGRMVGMGMTTATIIMHQGINTGGVFGHIFDFTSTPIIVRVILSLVICI
                     LLCTFFTMIAGFFTAKFKMHPFISTMANMLVIFGLVTYATKGVSFGAIEPTIPNMIIP
                     KINGFPTIIIWAAAAVFIVWFIWNKTTFGKNLYAVGGNAEAASVSGISVFAVTVGAFI
                     LAGILYGFGSWLECIRMVGSGSAAYGQGWEMDAIAACVVGGVSFTGGIGKISGVVVGV
                     FIFTALTYSLTILGIDTNLQFVFSGIIILVAVALDCLKYVQKK"
     gene            6295..7920
                     /locus_tag="ACZPD4_03690"
     CDS             6295..7920
                     /locus_tag="ACZPD4_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1378209.1"
                     /translation="MDKYTILLVDDEEEVIQAIIRKINWEELGFSVVGYADNGIKALE
                     MIEESQPDVVMTDIKMPYMDGMELCSHIRREYPAMKIVLFTGFDEFEYAKEAVHLEVE
                     EYILKPVNSVELINIFTKLKIKLDQEISERRSMEKLEHYYTESLPLLQANFCSTLIEG
                     RIHEDELQKYLSDYQISLPGPLYCCLVIHMSSSQIPENMNSLFLAASVQKQAEDRLGK
                     RWKAKCFPYLGNSVLFAQINNENEIAELTDDCDRFCKYVNRMMGTVVTVGIGQVCSQI
                     LDLAQSYNAAREAVSYRAVYGASRAINIKEIAPKESGEPCFTNDMELLNLFKKIRLNS
                     EEEIVEAVDQYIESISFPLKSLQQHHVVIMELVTALFRFSVNNDIAAEGVGGDIGILY
                     GKLLELEPDALRKWLTDISLCFSENLVTARSRSTQSFVSKAKEHVRSQYADVQLSLAQ
                     ICERLGVSNSYFSTVFKKETGKSFVSYVTDYRMEQAARLLIETNEKSYVIAQKVGYTD
                     PNYFSYVFKRKFGVSPSKYRAEHIKSEKQVFEK"
     gene            7934..>9678
                     /locus_tag="ACZPD4_03695"
     CDS             7934..>9678
                     /locus_tag="ACZPD4_03695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378210.1"
                     /translation="MQGENQNTEKSRITERVRSWEKYGGWTNLKPKGIQTTLMGVFSL
                     VSITIMLISGFVMYIWFSTLSREETVQSTQKLMEQTGESLEDYLVSMRQVSDTVYYNI
                     IKESDFSKQKQEIQSRMNLLYEANKDNLRSIAIYNHYGSLLAAEPVASQKEDPDVTQQ
                     DWYIQALDEVENMHFSTPHIQNLFDDGTQHYYWVTSLSRMIEITDSGVSNWGVLLVDM
                     DYADISRMMNQINTLNNGEYYYLCDSNGEIIYHPRQIQISNGLSGENSSLAASHKDGV
                     YEDDFKGERRKVIVNTISYTGWKLVGVIPYSAFIHGMIDVRYFIILLMLLTVMVLVAL
                     NRVVSARISSPILKLNASVLEYEAGEKPEVYIGGAEEIRYLGYSIQKSYEQIDELMKE
                     IIIEQNERRKSELDALQSQINPHFLYNTLDSITWMIEDGRNDEAAFMISQLAKLFRIS
                     LSKGHTVINIRDELQHARSYMNIQKIRYKNAFSVTFDVDDSLYAFCIVKLVLQPILEN
                     AINYGINGTDDCGEIKISGKMENGLIILSVTDNGIGMCSEEVSRLLTDEKRVHKHGSG
                     VGLVYYSSHFKVCECP"
BASE COUNT         3114 a         1534 c         2448 g         2582 t
ORIGIN      
        1 gaaggaaatc aggcgagtct ctatgtgata agaaatgaaa acgggatgga ggcagcagtg
       61 actgacttag gagcgacact ggtcagtttg aaggtgaaaa ataaagaggg aagcatggcg
      121 gacgttgttc tgggatatga aaacgcggcc ggttatgagg caggtacgtg ctttttcgga
      181 acgattgtag gaagaaatgc aaaccggatt ggcggcgcgc aatttgaatt aaacggaaaa
      241 acatatcatt tgacggggaa tgataacgga aataatctgc acagcggtat ggatttctac
      301 aatataagga tatgggagac ggaagaggtg acggagacgt ctgttcgttt taaacttcat
      361 agtccggatg gagatcaggg atatccggga acggcagata tatacgtgac atacagcctt
      421 gataaagatg ataagcttga tatcttttat gaagctttgt cggacaaaga tacgattctg
      481 aatatgacaa atcatagtta ttttaattta gatggacata acagcggaag tgtactgcag
      541 catacgctta ctctggaggc ggattatttt actccggggg atgcacagtc aataccgaca
      601 ggagagattc ttccggtgga gaatacacct atggattttc gggaaggaag aaaaatagga
      661 gagaggatcg aggaagagta cgaaccgctt cgattcggaa acggatatga tcacaactgg
      721 gttttgaaaa acaaaggaaa ctttgagaaa gtggcggaac ttatggctga ggaaagcgga
      781 atcgtgatgg aaacttacac ggatcgtccg ggaatgcaga tttatacggc gaactttgta
      841 gaaaatgaag taggtaaaga gggcgtcgta tatgggaaaa gaagcgcagt ttgtttagaa
      901 acacagtgtt atcctgatgc ggttcaccat gagaatttcc caagtcctgt ataccgtgca
      961 ggtgaaaaat ataccgcaca tactgcattt cgttttttga taaagaagta aaaacagatt
     1021 gaaaagacag atatgaaaca gaaagctgcc aaggggtata tcagcggctt tctgtttttt
     1081 gcattagtgg agaatgtaat ccgggaatgt gccttttaaa aatttcgatg attgtgtctg
     1141 actgaaaaag gatgggtaca aaatgacggg aaaataagag ctaaaatgac aaatattata
     1201 agacatgact aataaaatct taaaaaattc aatataaaca gaaaaatatt ctatgttata
     1261 tttaaaagaa ttcatataaa ctgtcattat caacaaaaaa tatctgtgcg aaaaagagca
     1321 gaagggagaa aatgtatgaa gagaaaaata gctttaatca caatggcggc ggtaatggca
     1381 ctgtcagtta ctgcgtgtgg aggaggatct gacagcggaa agtcagcgtc aaataattct
     1441 tccggatcgg aagtggcaaa taaggacaaa ccgttggtat ggttcaacag acagccgtca
     1501 aacagttcca cgggagaatt ggacatgaca gcattgaact tcaataaaga tacatattat
     1561 gtaggatttg atgcaaatca gggtgctgaa cttcagggaa ctatggtaaa agactatatc
     1621 gaaaaaaata tagataaaat tgaccgtaac ggtgacggag tgatcggata tgttctggct
     1681 atcggagata tcggacataa cgattctatc gcacgtacaa gaggtgtccg taaagctctt
     1741 ggtacaggcg tagagaaaga cggagatatc gacagcgctc cgatcggaac aaatgcagac
     1801 ggaactgcaa ctgctgtaaa ggacggaacg attgaagccg gcggaaagaa atatgtagta
     1861 agagaacttg cttctcagga gatgaagaac tctgcaggag caacatggga tgcggctaca
     1921 gcaggaaatt caatcggcac atggtcctct tcatttgggg aaaacataga cgttgtagtt
     1981 tccaacaatg acggaatggg aatgtcaatg tttaatgcat ggtcaaaaga taataaagta
     2041 cctactttcg gatacgatgc aaataatgac gcggtagctg ctattgcaga aggttacggc
     2101 ggaacgatca gccaacatgc agacgtacag gcatatttga cattgcgtgt tatccgtaat
     2161 gcacttgatg gcgtagatat tgatacaggt atcggtacag aagatgaagc aggcaatgtt
     2221 ttgtctgaag atgtatatac atacaatgaa gagcagcgtt cttactatgc gttgaatgta
     2281 gcggttacag ctgataacta tcaggaattt acagattcta caaaagtata tgagccggta
     2341 tccaaacagc tggatgagaa agaacatgcg aaaaagaaag tatggctgaa catttacaat
     2401 gcttccgata acttcttaag ttctacatac cagccgcttc ttaagaacta tgatgatctt
     2461 cttaatttag acgttgagta tatcggcggt gacggacaga cagagtccaa tattacaaac
     2521 cgtcttggaa atcctagcca gtatgatgcg tttgcgatta acatggtaaa aacagataat
     2581 gcggcttcct atacatcatt acttcagtaa taatgaggat aagcaggtct attagggaga
     2641 aggaattctc cctaatattt ttgcggcggc gataagctaa agtccgggtt tgcctgagac
     2701 tatggcggct gcctgcgatc tcggagagta ccttttggtg atttcggtaa ttgagatcag
     2761 gcagtcagag aaagttgtct gtgggaaaag gactgcaacc gtttgggtaa agatagaaaa
     2821 attctaaagt ataataggag taaaaagcat ggcagataag aaagataata tcgtcttatc
     2881 gatacgggat atgagtaagt cctttggaag aaacagagtt ctcactcgca tcaatctgga
     2941 tgtgaaagcg ggaacagtta tggggcttat gggggaaaac ggcgctggaa aatcaacgat
     3001 gatgaagtgt cttttcggta cctatcaaaa agacgaagga aatatcttcc tggatgggaa
     3061 agaggtaagt ttttccggac cgagggaggc gttggaaaac ggtatcgcta tggttcacca
     3121 ggaattgaat cagtgtctgg agagaaatgt agttgacaat ttgtttcttg gacggtatcc
     3181 ggtgaatgcg gtcggtgtaa tagatgaagg ccggatgaaa aaagaggcgt ctgaactttt
     3241 cagaaaattg ggaatgacag tcaacttgtc tcagccgatg cgtaatatgt cggtttctca
     3301 gcggcagatg tgcgagattg caaaagcaat ctcttataat tccaaggtga tcgttttgga
     3361 tgagcctacc tcttcattga cggtgcagga agtagaaaaa ctttttgaaa tgatgagaat
     3421 gctgaaagag cagggaattg ctttgattta tatttctcat aaaatggatg aaatttttga
     3481 aatttgtgat gaaatatcgg tgcttcgaga cggaaatctg gttatgacaa agagcacgga
     3541 agatacagat atgaatgaac tgattacggc aatggtagga cgttctcttg aaaaccgttt
     3601 tccggaagtg gataatacgc cgggagatgt ggttttgtcc gtgcagcatt tatctacaaa
     3661 attcgagcct tatcttaaag atgtgtcttt tgatgtgaga aaaggagaaa tcttcggatt
     3721 gtatggtctg gtaggagccg gacgaacaga gcttatggag acgattttcg gagtgcgcac
     3781 cagggcggca ggacgagtat actttaataa tcatcttatg aactttgaaa gtgcgaaaga
     3841 tgcgatggaa catggctttg ctatgatcac ggaggaacgt aaggcaaacg gattattttt
     3901 gaagggagat cttacgttta atacaacgat agcaaacttg aaacagtata aatcagggat
     3961 cgctctttct gattcgaaaa tggaaaaagc gactgcggaa gaaatcaaga ctatgcggac
     4021 aaaatgtatg ggaccaaatg atatgatttc aaatctttcc ggaggaaacc agcaaaaagt
     4081 tatttttgga aaatggctgg aaagagaacc gcaggtattt atgatggatg aaccgacaag
     4141 aggaattgat gtcggcgcaa aatatgagat atatgaactg attatcaaca tggcaaagca
     4201 gggcaggacg atcattgttg tctcgtctga aatgcctgaa attctgggaa ttacaaatcg
     4261 cattggcgtt atgtcaaatg gatatctttc tggaattgtt aatacaaaag agacaaatca
     4321 ggaagaacta ttaagactta gcgcaaaata cctataaaat gagggagaat gatgaatatg
     4381 gctaatggaa acagtaaggt tctgacggcg gaacaggaaa tgcagattcg ccgtccgata
     4441 gaagaatacg tcggtgcaat tcaaaaacag attgacggtc tccgggtgga cggcacggat
     4501 aaagtgttgt cgctgcaaaa cacaatggac ggagtaaaga gagatcgcac tcttacaaaa
     4561 ggtgaaaaag aggatagact tacgagaatg cgcagggaac tgcagcaggc aaaagcggta
     4621 gagtctaaga ataaagacag gatttcaaaa ctgattgcag atgcggaagc atatttgaaa
     4681 gaacatttcg acaaggaata ttatgtgcct gttaaagaaa gctgcgcaca ggaaaaagtc
     4741 cttgcgaaag agaaatatca aaaaagagta gaagaactga agaaagagca tcagcagatt
     4801 ctttccaaac tttcagaaca tcaggaaata aaggatgaaa aatatgttta taagaacagg
     4861 ctgttcgatg cgaaaatgga actgcaaaaa gattatcaga ctataaagga cagacggcat
     4921 gcggcgtatt cttataagta tcatctgatt gatttgctgc gtatgtcaaa atttactttt
     4981 cttgaaacgc gggcgcagaa atgggagaat tataaatata cgttcagtaa aagaaatttc
     5041 ttccttcaga acggattata tatcgctatt ctcttgatat ttattgtgct ttgtattatt
     5101 acgccgatga ttaagggagc gcctttgctc acttataata acgtgctgaa cgtacttcag
     5161 caggcatctc cgaagatgtt ccttgcatta ggtgttgcag gtttgattct tcttgcaggc
     5221 acagaccttt caatcgggcg aatggtaggt atgggaatga cgacggcaac gattattatg
     5281 catcagggaa ttaatacggg tggagttttt ggacatatat ttgatttcac gtcaacacct
     5341 atcattgtgc gtgtaatctt gtcattggtt atatgtatac ttttgtgtac attctttaca
     5401 atgatcgccg gattttttac tgccaagttt aagatgcacc cgtttatctc gacaatggca
     5461 aacatgctcg tgatcttcgg acttgttaca tatgctacaa aaggagtatc tttcggagct
     5521 atcgaaccga cgattccgaa tatgattatt ccaaagataa acggattccc tacaattatc
     5581 atatgggcag cagccgcagt ctttattgtg tggtttattt ggaataaaac aacttttggt
     5641 aagaatttgt atgcggtcgg cggaaatgca gaagcggcgt cggtgtcagg tatttcagta
     5701 tttgccgtta cagtaggagc atttattctg gcaggtattc tttacggatt cggctcctgg
     5761 ctggaatgta taagaatggt cggttcaggt tctgccgcat acggacaagg atgggaaatg
     5821 gatgcgattg cagcctgcgt agtaggcggc gtgtcattta ccggaggtat cggaaaaatc
     5881 tcgggagtag tagtcggtgt gtttattttc acagcgctta catattccct tacgatcctc
     5941 ggaattgaca caaaccttca gtttgtattc tcaggtatta tcattcttgt tgcggtagcg
     6001 ctggattgtc tgaaatatgt acagaaaaaa taaagataaa tcaaggaaag agccgacatt
     6061 atgtcggctc tttctgcttt aggataggtg atgagccaaa gccggagtct gcctgagatc
     6121 ccggcgatcg cttataattc cggaatgtgc tctttggtgt tctcggtgat tctgagccgg
     6181 tttaaaaaga cagaaagaac agacgacgga taaaatatga ggtcgtattt acaggcatgt
     6241 atgtctttgt tataatgagg aaaaataaaa cggaataata tatatggaga caagatggat
     6301 aaatatacaa tacttttggt tgatgatgaa gaggaagtaa tacaggctat tatccgaaaa
     6361 attaattggg aagagttggg attttctgta gtcggatatg ccgataatgg aatcaaggcg
     6421 ctggaaatga tagaggagag tcagccggat gttgttatga cagatattaa aatgccgtat
     6481 atggacggca tggagctgtg cagtcatatc agaagagagt atccggcgat gaagatcgta
     6541 ctttttacag gttttgatga gtttgaatat gcaaaggaag cggttcatct ggaagtggag
     6601 gaatatattt taaagccggt taattctgtg gaattgatca atatattcac aaagttaaaa
     6661 ataaagctgg atcaggagat aagtgaaaga agaagcatgg aaaagctgga acattattat
     6721 acggagtccc ttcctctttt gcaggcaaat ttttgttcta ctctgataga gggaaggatt
     6781 catgaggatg aacttcagaa atacctgtcg gattatcaga tttctcttcc gggtccatta
     6841 tactgctgtc tggtcattca tatgtcgtcc agtcagattc cggaaaatat gaattcctta
     6901 tttttagccg cgtctgtaca aaaacaggca gaagaccgtc tggggaaaag atggaaggca
     6961 aaatgttttc cctaccttgg aaacagcgtt ctgtttgccc agataaacaa tgaaaatgag
     7021 attgcggagc tgacagatga ctgcgaccgc ttttgtaagt atgtaaaccg catgatggga
     7081 actgttgtca cggtaggaat aggccaggtc tgcagtcaga ttttggattt ggcacaatcg
     7141 tataacgcag cgcgtgaagc ggtttcatat cgtgccgtct acggcgcttc cagggcgatt
     7201 aacattaagg aaatcgcgcc taaagagtcg ggagagccct gttttacaaa tgatatggaa
     7261 ttgttgaatt tatttaaaaa gattcgtttg aattcggaag aagagattgt agaagcggta
     7321 gatcagtata tagaaagcat ttcatttccg ctgaaatcat tgcagcagca tcatgtggtg
     7381 atcatggagt tggtaaccgc cttgtttcgg ttttctgtaa ataatgatat tgcagcggag
     7441 ggggtaggcg gagatatcgg aatactctat ggaaagctgc ttgagcttga acccgatgcg
     7501 ctgcgaaaat ggcttacgga tatcagcctt tgcttcagcg aaaatttggt tacggcaagg
     7561 agcagatcaa ctcagtcctt tgtatcaaaa gcgaaggagc atgtccgcag tcaatatgca
     7621 gatgtacagt tgtctctggc tcagatttgt gaaagacttg gggtgtctaa ttcctatttt
     7681 tctacagtgt ttaaaaagga aacaggaaaa tcttttgtca gttatgtgac agattaccgt
     7741 atggaacagg ctgcgcgcct gctgattgaa acaaatgaaa agagttatgt catagcgcag
     7801 aaagtaggat acacagatcc taattatttt agctatgtat ttaaaaggaa gtttggcgta
     7861 tcaccgtcaa aatacagagc ggagcatata aaaagtgaaa aacaagtatt tgaaaagtaa
     7921 atgcgggtat gatatgcaag gagaaaacca aaatacggaa aaaagccgca ttacggaacg
     7981 ggtgagaagc tgggaaaaat atgggggatg gacaaattta aaaccaaaag ggatacaaac
     8041 gactctgatg ggagtgtttt cgcttgtttc catcactatc atgttgattt ccgggtttgt
     8101 tatgtatata tggttttcta cgttatcaag ggaagagact gtgcagagta cccaaaagct
     8161 gatggaacag acgggggaga gtctggaaga ttatcttgtc agtatgagac aggtgtctga
     8221 taccgtttac tacaatatta taaaagagag cgatttctcc aaacaaaaac aggagattca
     8281 aagcaggatg aatcttttgt atgaggcaaa taaagataat cttcgaagta tcgcaattta
     8341 taatcattat ggaagtttat tggcggcaga accggtagcg tctcaaaaag aagatccgga
     8401 cgtaacccag caggactggt acatacaggc gttggatgaa gtggaaaata tgcatttttc
     8461 cacacctcat attcaaaatt tgtttgatga tggaacgcag cattattatt gggttacttc
     8521 tttaagccgt atgatcgaaa ttacagacag cggagtgtct aactgggggg ttctgcttgt
     8581 ggatatggat tatgcggata tttcaagaat gatgaatcag atcaatactt tgaacaatgg
     8641 agagtattat tatctgtgcg acagtaatgg ggaaatcatc tatcatccga ggcagataca
     8701 gatcagtaac ggtcttagcg gagaaaacag cagtctggca gcctcccaca aggacggtgt
     8761 gtacgaagat gattttaaag gcgagaggcg gaaagtaata gtcaatacta tcagttatac
     8821 cggatggaag ctggtggggg taattcctta ttcggcattt atacacggga tgatcgacgt
     8881 gcgatatttt attattttgt tgatgcttct gacggtaatg gtacttgtag cgcttaatcg
     8941 tgtcgtttca gcgagaatct caagcccgat tttgaaatta aacgcatcag tactggagta
     9001 tgaagcgggt gaaaagccgg aggtatatat tggaggagcg gaagaaatcc ggtatctcgg
     9061 atattctatc caaaaatctt atgagcagat agatgagctt atgaaggaga ttattatcga
     9121 acaaaacgaa cgtagaaaaa gcgagttgga tgcattgcaa agccagatta atcctcattt
     9181 tctttataac acgctggatt ctatcacgtg gatgatcgag gatggacgca acgacgaggc
     9241 ggcgtttatg atttcacagc ttgcaaaatt gttccgtata agtttatcaa aagggcatac
     9301 tgtgattaat atccgggatg agctgcagca tgcccgaagt tatatgaata tacagaagat
     9361 acgatataaa aatgcctttt cagttacttt cgatgtagat gattctcttt atgcattttg
     9421 tattgtaaaa ctggttttac agccgatctt ggaaaatgcg atcaattacg gaatcaatgg
     9481 gacagatgac tgcggtgaaa taaaaatcag cggtaagatg gagaatgggc ttattattct
     9541 ttcggtgaca gataacggga tcggtatgtg cagtgaggaa gtgagccggc ttttgacaga
     9601 tgagaaaaga gtacataagc atggttcggg agtaggactt gtttactaca gtagccattt
     9661 caaagtttgt gaatgccc
//
LOCUS       DBMDXF010000057         9627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1940_length_9627_cov_2.443523, whole genome shotgun sequence.
ACCESSION   DBMDXF010000057 DBMDXF010000000
VERSION     DBMDXF010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_9627_cov_2.443523"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            256..882
                     /locus_tag="ACZPD4_03700"
     CDS             256..882
                     /locus_tag="ACZPD4_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR1378211.1"
                     /translation="MEYLSALKKSVVAGLMIGIGCTVFLNMDNSIVASFLFGLGLFTI
                     INLELNLFTGKIGYICKENCAETLITLVGNGIGVNIMAFLMKQTRVGVRLVEKAGPIV
                     ETKLSDTYISLFLLAVCCGMLMYIAVATFKKQPNILGTIAVFLCVSVFILAGFEHCIA
                     NMFYFGLVSTPTKYAVPLLIMILGNSTGGILLCKLTQHVQIQKNSENA"
     gene            1020..2222
                     /gene="coaBC"
                     /locus_tag="ACZPD4_03705"
     CDS             1020..2222
                     /gene="coaBC"
                     /locus_tag="ACZPD4_03705"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1378212.1"
                     /translation="MLKDKTVLLGVTGSIAAYKIAYLASALKKLHADVHVLMTKNAVN
                     FINPITFETLTGNKCLVDTFDRNFEYSVEHVSLAKKADIVMIAPASANVIGKVAHGIA
                     DDMLTTTVMACRCKKFIAPAMNTNMYENPIVQDNLKILQGYGYGVVDPAVGYLACGDT
                     GAGKMVEPDVLLDVILQEIAYEKDLAGLKILVTAGPTQEAVDPVRYITNHSTGKMGYA
                     IAKIASRRGAEVTLVSGPTAEEEPAFVNVVNVCSAKEMFEAVKERAQEQDIIIKAAAV
                     ADYRPKHIRGSKMKKTEDGSDLTLELERTDDILAYLGAHKKQGQFLCGFAMETEDLIA
                     NAKRKLEKKRLDMIAANSLRVEGAGFGGNTNVVTVITKEKEVSLGKMTKEETAAELLT
                     MIAEARKV"
     gene            2332..3147
                     /locus_tag="ACZPD4_03710"
     CDS             2332..3147
                     /locus_tag="ACZPD4_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1378213.1"
                     /translation="MENKTVTENSTVSCAEEMNTAEVYGSDQYAADLKTASKDRQTSG
                     KQRSSSKFSTMSMVQVAIFGAVICVMAFTPFLGYIPLGFTRATIIHIPVIIASLLMGP
                     KKGGALGFLFGLTSFINNTMNPTPTSFVFTPFYSIAGVSGGWGSVIICFLPRILVGII
                     PYYVYKLIIRFSNEKTKKTGVSSIGLVTAGVAGAMTNTLLVMNLIFLFFREAYASANN
                     VAVSGVYTFILSIIGINGVPEAIVAGVLTLCIGRALLKKNVRERLGFRNDIGY"
     gene            3131..3898
                     /locus_tag="ACZPD4_03715"
                     /pseudo
     CDS             3131..3898
                     /locus_tag="ACZPD4_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
     gene            4175..>9627
                     /locus_tag="ACZPD4_03720"
     CDS             4175..>9627
                     /locus_tag="ACZPD4_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 95 catalytic
                     domain-containing protein"
                     /protein_id="HIR1378214.1"
                     /translation="MKRKLSKAGGKALSVALAMATVVSAAPFSVAAEDNVALAQTQET
                     EGNALRLWYDEEAPNSYTGWEQWALPLGNSAIGASVFGGVQTERIQLNEKSLWSGGPS
                     DSRPEYNGGNIESKGQNGKVMAQLKEKLKSGQGFDSNLAGQLIGVSDDAGVQGYGYYL
                     SYGNMYLDFKNVTKNNVSGYSRDLDLRTAVAGVNYDLNGAHYTRENFVSYPDNVLVTR
                     LTATDGGTLDFDVRVEPDEEKGGSQNQPGADSYARMFDKKVSDNAIAIDGQLKDNQLK
                     FSSYTKVIKDDGTAGQIKDDSTNGKITVSGAKAITIITSIGTDYKNDYPKYRTGETKE
                     QLAALVKGYVSGAEAKVKAGGYETLKEDHVNDYDHIFGRLDLNIGQAVSDKTTDKLLE
                     AYKKGTASETEKRYLELMLFQYGRYLTMGSSRETPVNEDGTKNERRATLPSNLQGIWV
                     GANNSAWHSDYHMNVNLQMNYWPTYTTNMAECAEPLINYVDSLREPGRITAKIYAGVE
                     STEANPENGFMAHTQNNPYGWTNPGWVFDWGWSPAGVPWILQNCWEYYEFTGDTEYMQ
                     THIYPMMKEEATLYDQMLMRDNDGKLVSVPSYSPEHGPRTAGNTYEHSLIWQLYEDTI
                     TAAETLGVDEAKVAQWKKNQADLKGPIEVGASGQIKEWYNETTLNTDENGNQMGQGYG
                     HRHISHMLGLYPGDLIAQSDEWLAAAKVSMQNRTDETTGWAMAQRVATWARLAEGDKA
                     YDVLSKMVTSGKIMTNLWDTHAPFQIDGNFGYTAAVAEMLVQSNMGHIDLMPAVPKAW
                     GTGNVKGLLARGNFAVDMAWADNKLTEASIHSNNGGEAVVQYANLSLATVKDSDGNLV
                     EITPVTSDRISFNTEAGKTYTITAIPDNTLAAAPTGLKVTKIKDGETVLTWDAVKART
                     EVSYNVYRQIEGGEWVQVQTELKATEWTDTDAWDVLGTLKYKVTAVIDGKESEFSSEA
                     TVDDCRNMIGMIDDQDERIKYTGSWGNWISQEGNYGGSVKYLEDPTGTETAELTFCGT
                     GIEVFSCTQNDRGKVEISIDGEVWATADTYSATQKRQAKIFSTEEHKDKTLEYGIHTV
                     KVRAMGEKNASASRDKVELDAFNVLNTEAARVEKVEVASASGMTTVSKAGSTLQMKVA
                     VTPAEAINKGVTWSVTTKSGSAAATIDENGLLSAGTANGVVTVKATSKENAEISGTCE
                     ITVAIPDVTAQSEIIEDCSDDKNSKNPKITWSETGWSNPYTGEADKHHGGTKTETSTE
                     GAWFSYTFTGTGIKFYAQKNNTQGKFTVELDGKAESDAVLWEEKSGGSPQQCVFEKTG
                     LENKEHTIKFTAARDHNNINVNIDYLEVFAPAAASVDKSALQAAIEKYSGLNKDDYAE
                     SLWNEFKKAYDDAVKGMNDDKTTKEQADKLAADLNAAGVKLEAAEIPAPTIPEDAKAT
                     VMGVTSTEATVSWTKLPRAESYKVYAVEHEGTAKALKAITDTVPDNAGEPVTTTETTV
                     RFTNLKPGTSYVFMIVGVNHNKVESEKALCSEPFTTVAAADEKAPAQVSGISVVKEGE
                     KDVKITWAPSEDPEGSEVTYIVYVDGVIVSESDKTEYTLKNADESKDYSIRIVAVDAS
                     GNKAVPAVINLTVDDWNAKTVVGVEKLADLKVKKGTAADKLDLPKTVNVTLEGGRVAD
                     VLEVKWTTDNYKADQIGTQTLSGELQKKKGVKIPDKFKTVTINVIVEESEVNPPQPED
                     NVVTDVEELTDLTVEFGTSVEKLALPTEVKVTLDNETEDKLSVEWNTDAYKADEAGTY
                     ELTGTLQAKEGIKLPDNFKTVTINVTVEEKGGEV"
BASE COUNT         3149 a         1725 c         2525 g         2228 t
ORIGIN      
        1 agggggatag ttgtttatca actatctgat acttagtata aagagaaaat atgtctaaag
       61 tatgttgaaa gaataaaaga atacgaaaat ttctaaacag aagattaata aaacggaaaa
      121 agagttctat acatttgcgg tatttatggt ataataggca cgttgtgcct gcgagccggg
      181 gcggtatcgt cgggttttat acaacattta attaagaata cttacaattt tagaagaaaa
      241 agaacgaggt gaggtatgga atatttatca gctttaaaaa aatctgttgt tgccgggttg
      301 atgatcggga tcggctgtac tgtttttttg aatatggaca attcaatcgt ggcgtcattt
      361 ttattcggat taggactttt tacgattatc aatttggaat tgaatttgtt taccggcaag
      421 atcggatata tctgtaaaga aaattgtgcc gagacattga ttactttggt cggaaacgga
      481 atcggtgtta atattatggc gtttcttatg aaacaaaccc gtgtcggagt aagattggtt
      541 gagaaggcgg gtccgatcgt agaaacgaaa ttgtcagata catatattag tttgtttttg
      601 ctggctgttt gttgtggaat gctgatgtat attgccgtgg caacatttaa aaaacagccg
      661 aatattttag gaacgatcgc agtctttctt tgcgtttcgg tctttatttt ggcaggattc
      721 gagcactgta ttgcaaatat gttttatttt ggtcttgtaa gtacaccgac aaaatatgct
      781 gttccgcttc ttattatgat tctcggaaac agtacgggag gtattttgct gtgtaagctt
      841 acgcagcatg tgcagattca gaaaaactcg gaaaatgcgt aatacagaca gtgattttct
      901 ttgagaatac atttcctgaa aaaaggcgga tctcaacctg aacatacgat ttgttcaggt
      961 tgatttttta tatacgatac aatataatga agcggagcaa aaggaaaggg ggtacgaaaa
     1021 tgttaaaaga caaaacagtt ttgctcggag ttaccggaag tatcgctgcg tataagatcg
     1081 cgtatcttgc cagtgcgctg aaaaagcttc atgcggacgt acatgtgctt atgacgaaaa
     1141 atgcggttaa ttttatcaat ccgatcacgt ttgaaacgct gacgggaaat aagtgtcttg
     1201 tagacacgtt tgaccgtaat tttgagtata gcgtggagca tgtgtctctg gcaaagaaag
     1261 cggatatcgt aatgatcgca ccggcaagcg ccaacgtgat cgggaaagtc gcccacggca
     1321 tagcggacga tatgctgaca acaacggtta tggcatgcag atgtaagaaa tttatcgcgc
     1381 ctgcgatgaa cacgaacatg tatgaaaatc ctatcgtgca ggacaactta aagatcttgc
     1441 agggatacgg atacggtgtt gtagatccgg cagtgggata ccttgcatgc ggagataccg
     1501 gagccggaaa gatggttgag ccggacgttc tgctcgatgt cattttgcag gaaatcgcat
     1561 atgaaaaaga tctggcaggt ttgaagatcc ttgtaacggc aggcccgaca caggaagcgg
     1621 tcgatccggt acggtatatt acaaaccatt ctaccggaaa gatgggttat gctattgcaa
     1681 aaatcgcatc aaggagaggc gctgaggtga cgctcgtatc agggccaact gccgaggaag
     1741 aacctgcatt tgtaaatgtt gtaaatgtat gttccgcaaa ggagatgttc gaggcagtta
     1801 aggaacgggc gcaggaacag gatatcatta tcaaagcggc agcagtggcg gattacagac
     1861 cgaaacacat aagaggcagc aagatgaaaa agacagaaga cggatcggat ctgacgcttg
     1921 agctggaacg cacagacgat attttggctt atctcggagc acacaaaaag caggggcagt
     1981 ttttatgcgg gtttgcaatg gaaacggaag acttgattgc aaatgcaaaa agaaaactgg
     2041 aaaagaagcg gcttgacatg atcgccgcaa acagtcttcg tgtggaaggc gcaggattcg
     2101 gcggaaatac aaatgtcgta acggtcatca cgaaagagaa ggaagtttca ctcggaaaaa
     2161 tgacaaaaga ggagacagcc gcagaacttt tgacgatgat tgcagaagcc agaaaagtct
     2221 gataagaaac agacaaaaag caaatgtatt gtatcccgaa agggaacgat aagaagcaga
     2281 aaaaagcaaa tgtattgtat cccgaaaggg aatgataaca aggaggcaga catggaaaac
     2341 aaaacagtaa cagaaaacag cacagtatca tgtgcggaag aaatgaatac ggcagaagta
     2401 tacggctcag atcagtatgc agcggatcta aagaccgcga gcaaagatcg gcagacgtcg
     2461 gggaaacagc gctcgtcaag taaattcagt acaatgagta tggttcaggt ggcaatattc
     2521 ggagctgtca tatgtgtgat ggcttttacc cctttcctcg gatatatccc gcttggattt
     2581 acccgtgcga cgatcataca tatcccggtt attatcgctt cgcttttgat gggaccgaaa
     2641 aaaggcggcg ctttaggatt cctttttgga ttgacaagtt ttattaacaa tacgatgaat
     2701 ccgacaccga catcgtttgt atttactccg ttttacagta ttgcaggcgt aagcggcgga
     2761 tggggaagtg tgattatctg ttttcttccc cgcatacttg tgggaattat accgtattat
     2821 gtatataagc ttatcattcg tttttcaaat gaaaaaacaa aaaagaccgg tgtgtcaagc
     2881 attggacttg tgactgcagg cgtggcgggc gcaatgacaa atacgctgct tgttatgaat
     2941 ctgatttttc tgtttttcag ggaagcatat gcatcggcaa ataatgtggc ggtatcgggc
     3001 gtatacacat ttattctttc tattatcggg ataaacggcg ttccggaagc aatcgtggca
     3061 ggtgtgctga ctttatgtat cggaagagcg cttttgaaaa agaatgtaag agaaaggctt
     3121 ggttttagaa atgatattgg ctattaatat cggaaataca aacaccgtgc tcggctgcat
     3181 tgacggctca aaatgcgtgt tcgtagaacg gatttcaact gttaagacaa agacggaact
     3241 ggagtatgcg atcgatatta aaaacgtact cgacatctac catattaaaa aggcagattt
     3301 agaaggcggt atcatttcgt cggttgttcc gcagattacg atggttgtaa aagctgcggt
     3361 tgaaaagatt taaaaaaagg aagtccttat cgtaggcgcg gggatcaaga ccggattaaa
     3421 tatcaggatc gacaatccgg cgcagcttgg cagcgacctt gttgtcggag cggtggcagg
     3481 gattgcggaa tatccgacgc ctcttcttat ttttgatatg ggtacggcaa acacggtttg
     3541 tgttatagat aaaaaccgaa actatatcgg cggcatgatt tatccgggga tcggagtttc
     3601 tcttgattcc cttacagcca atgcgtctca gcttggcggg atcagtctgg aagaaccggg
     3661 gcatattata ggaaaaaata cggtggactg tatgaaaagc ggtatcgttt acagcaacgc
     3721 tgcggcgatc gacgggttga tcgatcgact gtcagaagaa gtggacggcg atgtaacggt
     3781 agttgcgaca gggggagtgg cggaaaaaat cattcctcat tgcagaagaa agattattcg
     3841 agacgatgat cttgttttga aaggactgga aatcatctat cggaagaact gcaggtgaca
     3901 gatcggagaa aatcgacctg tgtataaggt aaatataacg taaaaagaga aaaggataga
     3961 cgcggtatag actatatata caaaaaatgg ctgcggctat ccttgaattt atgtgtaaaa
     4021 aagcgaaaat ataaaaaact tactatttta aatcataaaa atataacaaa atataataaa
     4081 agtgtgatat gataaacagt acaacgtagc atcggagtat tcggcttaat ttatttatgg
     4141 ctgtacttct gtgcgaattt aaaggaggaa aaagatgaaa aggaagcttt caaaagcagg
     4201 cgggaaagcg ttatctgtcg cacttgcgat ggcgactgtt gtatcggcag ctcctttctc
     4261 tgtagcggcc gaggataacg ttgctttggc acagacacag gaaacggagg gaaatgcttt
     4321 gcgtttgtgg tatgatgaag aggcacccaa ttcgtatacg gggtgggagc agtgggctct
     4381 tccgcttgga aacagcgcga ttggagccag tgtgtttggc ggtgttcaga cagagcgaat
     4441 tcagttgaat gaaaagtcgc tttggtccgg cggtccttca gacagccgac cggaatataa
     4501 cggcggtaat attgaatcaa aaggtcagaa cggcaaggtt atggcacaac tgaaagagaa
     4561 actgaaaagc ggacaagggt ttgacagtaa tcttgcagga cagcttatcg gagtcagtga
     4621 tgacgccgga gtacagggat acggatatta tttaagttat ggcaatatgt atctggattt
     4681 caaaaatgtt acaaagaata acgtttcggg atattcgcgc gatctggatc ttcgcacggc
     4741 ggtagccgga gtgaattatg atctcaacgg agcgcactat acaagagaaa acttcgtaag
     4801 ctatcctgac aacgttcttg taacacgtct gactgcaaca gacggaggaa cgcttgattt
     4861 tgacgttcgt gtagaaccgg atgaggaaaa aggcggaagt caaaaccagc cgggagcaga
     4921 ttcttacgcg agaatgtttg acaaaaaagt atccgacaat gcaatcgcaa tcgacggaca
     4981 gcttaaagat aaccagttga agttcagttc ttatacgaag gtaatcaaag acgatggaac
     5041 agcagggcag atcaaagacg attcgacaaa cggtaagatc acggtaagcg gtgcgaaggc
     5101 gatcacgatc attacttcca tcggaacgga ttataaaaac gattatccga aataccgtac
     5161 aggcgagaca aaagaacagc tcgctgcttt ggtaaaagga tatgtatccg gagctgaggc
     5221 gaaagtaaaa gccggcggat atgagacgtt aaaagaagat catgtaaatg attacgatca
     5281 tattttcgga agattagatc ttaacatcgg acaggcagtg tctgacaaga caacggataa
     5341 gctgttggag gcatataaaa aaggaacggc ttccgaaaca gaaaaacgtt atctggaatt
     5401 gatgctcttc cagtacggac gttatctgac gatgggatca tcgagagaga ctcctgtgaa
     5461 cgaagacgga acgaagaatg aaagaagagc aactcttccg tcgaacttgc agggaatctg
     5521 ggtcggagcg aataactccg catggcattc ggactatcat atgaatgtca atctgcagat
     5581 gaactactgg ccgacatata cgacgaatat ggcggaatgc gcagaaccgc tgattaatta
     5641 cgtagactct cttagagagc ctggacgtat tacggcgaag atttacgcgg gcgttgagag
     5701 tacggaagca aatcctgaaa atggatttat ggcgcataca cagaacaacc cgtacggatg
     5761 gacgaacccg ggctgggtat ttgactgggg atggtctccg gcaggcgttc cgtggattct
     5821 tcagaactgt tgggaatatt atgagtttac gggagataca gagtacatgc agactcatat
     5881 ttacccgatg atgaaagaag aagcgacgct ctacgatcaa atgttgatgc gcgataatga
     5941 tggaaagctt gtatctgttc cgagttattc accggagcac ggaccgagaa ctgcaggtaa
     6001 tacatatgag cattcgttga tctggcagtt atatgaagat acgatcacag cggcagagac
     6061 tttgggagtt gacgaagcga aagtcgcaca gtggaaaaag aatcaggctg acttaaaagg
     6121 accgatcgag gtcggtgcca gcggacagat caaagagtgg tataacgaga caacgttaaa
     6181 tacagatgag aacggtaatc aaatgggaca aggatatggt catcgtcata tttcccatat
     6241 gctcggatta tatccgggag acttgatcgc tcagagcgac gaatggctgg cggcagcgaa
     6301 agtatcgatg cagaatagaa ctgatgaaac gacaggatgg gcaatggctc agcgtgtcgc
     6361 aacatgggcg agacttgcag aaggcgataa agcatacgac gtattgtcca aaatggtcac
     6421 gagcggcaag atcatgacaa acctgtggga tacacacgct ccgttccaga tcgacggaaa
     6481 cttcggttac acagcggcag ttgcggaaat gcttgttcag agcaacatgg gtcacatcga
     6541 cctgatgcct gcagttccga aagcatgggg aacaggtaat gtgaaaggtc tgcttgcccg
     6601 cggtaatttt gcggttgata tggcatgggc agacaacaaa ctgacagaag cttcgatcca
     6661 ctctaataac ggcggagaag cggttgttca gtacgcaaat ctttcacttg caactgtaaa
     6721 agacagtgac ggaaacctcg ttgagatcac accggtgact tcagatcgta tttccttcaa
     6781 tacagaagca ggaaagacat atacgatcac tgctatccct gataatacat tagctgcagc
     6841 tcctacaggt cttaaggtaa cgaagatcaa agacggagag acagttctta catgggatgc
     6901 ggtcaaagcc agaacagaag tatcttataa tgtataccgt cagattgaag gcggcgagtg
     6961 ggtacaggtt cagacagaac tgaaagcgac agaatggaca gacacagacg cttgggatgt
     7021 gctcggaaca ttgaagtaca aagttacggc tgtgatcgac ggaaaagaaa gtgagttcag
     7081 ttcggaagca acggtagatg actgccgtaa catgatcggc atgatcgacg atcaggacga
     7141 acgtatcaaa tatacaggaa gctggggcaa ctggatcagc caggaaggaa actacggcgg
     7201 ttcagtgaaa tacttagaag acccgacagg aacagagaca gcggaactta cattctgcgg
     7261 aacgggtatc gaagtattct cctgtacaca aaacgacaga ggtaaagttg aaatctctat
     7321 cgacggagaa gtatgggcaa cagcagatac ttattcggca actcaaaaaa gacaggcaaa
     7381 aatattctct acagaagagc ataaagataa gacgcttgaa tacggaatcc atacggttaa
     7441 agttcgggcg atgggcgaga aaaatgcatc tgcaagcaga gataaagtag agcttgacgc
     7501 gtttaacgta ttgaatacag aagcggcgcg ggttgaaaaa gtagaggtag catctgcaag
     7561 cggaatgaca acagtaagca aagccggatc gacgcttcag atgaaagtgg cagtaactcc
     7621 tgcagaggcg atcaataaag gtgttacgtg gtctgttaca acaaaatcag gcagcgcggc
     7681 agctacgatc gatgaaaacg gtcttctgtc agcaggaaca gcaaacggtg ttgtgacagt
     7741 aaaagcgaca tcgaaagaaa atgccgaaat ctcaggcaca tgcgagatta cagttgcaat
     7801 cccggatgtg acagcgcagt cagagatcat cgaggactgc tcggatgata agaactccaa
     7861 gaatccgaag atcacatgga gtgaaacagg ctggagtaat ccgtacacag gagaagctga
     7921 taagcatcac ggcggaacta agacggagac aagtacagaa ggagcatggt tcagctatac
     7981 atttacagga acaggcatta agttctatgc tcagaaaaat aatacgcaag gcaaatttac
     8041 tgtagaactg gacggaaagg cagaaagcga cgcagttctt tgggaagaaa aatccggcgg
     8101 atcaccgcag cagtgcgtat ttgaaaagac aggtcttgaa aataaagaac atacgattaa
     8161 gtttacagcg gcaagagatc ataacaatat taatgtaaac atcgattacc ttgaagtgtt
     8221 tgctccggca gcagcttctg ttgacaaatc ggcgcttcag gctgcgatcg agaaatacag
     8281 cggtctgaat aaagacgatt acgcagaaag tttatggaat gaatttaaga aagcgtatga
     8341 cgatgccgtt aaaggaatga atgacgacaa gacgacaaaa gagcaggctg ataagcttgc
     8401 agcagatctg aatgcagcag gcgtaaaact tgaagcggca gaaattccgg ctccgacgat
     8461 tccggaagat gcaaaagcaa ctgtaatggg agttacaagt acagaagcaa ctgtttcatg
     8521 gacgaaactt ccaagggcag agtcttataa agtatatgct gtagaacatg aaggaacagc
     8581 aaaagcgctg aaagcgatta cagatactgt tccggataat gcaggagaac ctgtgacaac
     8641 aacagagaca acggtacggt ttacgaatct gaaaccgggt acatcttatg tatttatgat
     8701 cgtaggagta aatcataata aagtagagtc ggaaaaagct ctttgcagcg agccgtttac
     8761 gacagtggcg gcggcagatg aaaaagctcc ggcacaggta agtggaatca gcgtagtaaa
     8821 agaaggtgaa aaagatgtga agatcacatg ggctccgtca gaagatccgg aaggaagcga
     8881 agttacatat attgtatatg tagacggcgt gatcgtatct gaaagcgata agacagaata
     8941 tacattgaaa aacgcggatg aaagcaaaga ctacagtatc cgtatcgtag ctgtagatgc
     9001 atcaggaaat aaagctgtac ctgcagtaat taacctgacg gtagacgact ggaatgcaaa
     9061 gacagttgta ggcgtagaga aacttgccga tcttaaagtg aagaaaggaa ctgctgctga
     9121 taaactggat cttccgaaaa cagtaaatgt tacactggaa ggcggtcgtg ttgcagatgt
     9181 acttgaagta aaatggacga cagataatta caaagcagat cagatcggaa cacagacgct
     9241 gtcaggagaa cttcagaaga agaaaggcgt taagattcct gataagttta agacagtaac
     9301 gatcaacgta attgtagaag agagcgaagt aaatccgccg cagccggaag acaacgtagt
     9361 tacagacgta gaagaactta ctgatcttac ggttgagttc ggaacttcag ttgagaaact
     9421 tgctcttccg acagaagtaa aagtaacatt agataacgag acagaagaca aactgtcagt
     9481 agagtggaac acagatgcgt ataaagcaga cgaagcggga acgtacgaac tgacaggaac
     9541 acttcaggca aaagaaggaa ttaaacttcc tgacaacttc aagacagtaa cgatcaacgt
     9601 aactgtagaa gaaaaaggcg gagaagt
//
LOCUS       DBMDXF010000058         9622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1944_length_9622_cov_1.808739, whole genome shotgun sequence.
ACCESSION   DBMDXF010000058 DBMDXF010000000
VERSION     DBMDXF010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_9622_cov_1.808739"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            76..2439
                     /locus_tag="ACZPD4_03725"
     CDS             76..2439
                     /locus_tag="ACZPD4_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR1378215.1"
                     /translation="MKRRPLCIFCVLFLTVQAIRVLFFGAGDYTSALGQIVNRNNKGE
                     INLILTGTADRIDEKESVTAVRVKGNAVSFYSGSDRKIARQSVFGKSDGHRSSYDFSQ
                     ELPRKIWEEKVLVYLEKEDAAHIKIGNKLKIKGKASAFETARNPGNFDQRAYYKRQKI
                     GAYVWADEVGILSGQTECIRQFLHEFRQGWKKLLVGCLGSYYGNTISAILLGEKGDLD
                     PYMKKLYQKNGIGHLLAISGLHMSFIGSGLYGFLRKRGVGFPVSGAVGTIVLLFYTVM
                     TGASISALRALIMFSVRMGAEVTGRDCDLPTSLAVAAALLSAKDPLCLSDAGFLFSFG
                     SLCGICMLTPLFECLFGCGTEKKNIWTHMGKAVSASLAVNLFLLGPMLYFYFEIPPYS
                     VFLNLFVIPALPVIMGTGLSGSVMILLVKPIGIILLKSSGVILWAYDRLCSAAVMLPF
                     GRITAGKPSVQLLVAYYVILFALYGMYRRFPKKIWGYMLFVSAAFLIVLCGAKTRDIE
                     GIKITVLDVGQGDGIVLSGKGKNYLIDGGSSDVKQAGAQRIEPYLLSEGIGCLDYVFV
                     THGDEDHISGIRELLEGQKLGVRIDTLVLPPEEYHDEKLADLARIAVENGTRVVSVKT
                     GANIYGRGERQTEKNDSKEVMRLRCIGPLTDIPQGLTKPKAGNEASLVLEFSYGNFDM
                     LFTGDVEGAGEELLVKSDVLRKYEILKCAHHGSKNSGTAAFLEKTDPRAAIISAGIDN
                     RYGHPHEETLNRLKKRKVKTYNTQTDGAVTIKSDGIQISIESFLLSK"
     gene            2467..3450
                     /gene="holA"
                     /locus_tag="ACZPD4_03730"
     CDS             2467..3450
                     /gene="holA"
                     /locus_tag="ACZPD4_03730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1378216.1"
                     /translation="MKSLNEDLKTGQFKQIYLLYGEESYLKRQYRERLVRAMIPEGDT
                     MNYAVYEGKNIDIKEVIDLAETLPFFASRRLIVFEDTGFFKNAGTDLADYITDIPPTT
                     YFLFIEKEVDKRNRLYKAVKAKGHIVELTVQDENTLRRWVLGLVKKEKKQMEGADAAY
                     FLNKVGTDMENITQELEKLFCYTIGRDRITRDDIDQICVTQITNHIFEMVNAVAGKDQ
                     KRALDLYYELLALKEPPMRILFLMTREYRLLFHVKGLMVNGYGKKEIASKAGIHPFAA
                     GRYMDAAKRYELGELRTVMEEGAQLEQNVKTGLMTDTLAVELFIVKNSGKQ"
     gene            3512..5326
                     /gene="lepA"
                     /locus_tag="ACZPD4_03735"
     CDS             3512..5326
                     /gene="lepA"
                     /locus_tag="ACZPD4_03735"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1378217.1"
                     /translation="MSVIDQSKIRNFCIIAHIDHGKSTLADRIIEQTGLLTSREMQSQ
                     VLDNMELERERGITIKAQTVRTVYKADDGEEYMFNLIDTPGHVDFNYEVSRSLAACDG
                     AILVVDAAQGVEAQTLANVYLALDHDLDVIPVINKIDLPSADPERVKEEIEDVIGIEA
                     EDAPLISAKTGQNIHDVLEQIVKKVPAPQGDPKAPLKALIFDSVYDSYKGVIVFCRIK
                     EGTVKKGTPMLMMATGAQADVVEVGTFGAGRFIPCEELSAGMVGYITASLKNVKETRV
                     GDTVTDASRPCDSPLPGYKKVNPMVYCGLYPADGAKYPDLRDALEKLQLNDAALQFEA
                     ETSVALGFGFRCGFLGLLHLEIIQERLEREYNLDLVTTAPSVIYKIHKTNGEVIDLTN
                     PTNMPDPAEIDYMEEPMVKAEIMVTSEYIGAIMDLCQERRGIYQGMEYIEEKRAVLNY
                     HLPLNEIIYDFFDALKSRSRGYASFDYEMMGYQKSELVKLDILINKEEVDALSFIVFA
                     GSAYDRGRKMCAKLKEEIPRQLFEIPIQAAVGSKIIARETVKAMRKDVLAKCYGGDIS
                     RKRKLLEKQKEGKKRMRQVGSVEIPQKAFMSVLKLDDE"
     gene            5392..6663
                     /gene="hemW"
                     /locus_tag="ACZPD4_03740"
     CDS             5392..6663
                     /gene="hemW"
                     /locus_tag="ACZPD4_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1378218.1"
                     /translation="MKKELELYVHIPFCVKKCAYCDFLSFSAKQEEVSAYVEALAEEI
                     KGKKEQFSDYCVTTIFLGGGTPSILEGVYTASIFRALRESFDIAENAEITMEVNPGTV
                     SEEKINMWKTCGVNRLSIGLQSVDDGELKMLGRIHTYDEFLTTWKMVRKAGFHNVNID
                     LISAIPGQTKKSWETTLRTVAQLQPEHISAYSLIIEEGTVFGTIYGEDGERICQKELL
                     SRESSMPEVSDKDGNIRKDRQILPLPDEDTERAIYEITEKILREYGYERYEISNYAKP
                     GYECRHNLGYWERREYLGLGLGASSLIAEHRFCNTDKMDVYIRLNKTVDPDGKKEESK
                     KAVWEKAEEVSVLSVQEQMEEFMFLGLRKTDGVSEKSFFETFGVQIDTVYGETIGKLE
                     KERLLIKEKGQIRLTERGVDISNYVMSEFLF"
     gene            6859..7908
                     /gene="hrcA"
                     /locus_tag="ACZPD4_03745"
     CDS             6859..7908
                     /gene="hrcA"
                     /locus_tag="ACZPD4_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1378219.1"
                     /translation="MAVDTSLSERKLTILKAIIQNYLETGEPVGSRTLSKYTDLNLSS
                     ATIRNEMADLEDLGYIFQPHTSAGRIPSDKGYRLYVDMLMEEKEQEIAKRESAMLEKT
                     DKVEKVLQQAARVLATNTNYATMVSSPINNRNTLKFIQLSQVDAEQIVAVIVLGGNVI
                     KNKIIEVGEMLSNENLLKLNMLLNTTLNGLPIDQITLGLIARLKEQAGIHSEVVAHVL
                     DAVAETIHVEEDMKIYTSGATNIFKYPELSDKQSAQEIISAFEEKQQLADLVTETLAS
                     EDSHGIQVYIGDETPVKTMKDCSVVTATYELGEGMKGTIGIIGPKRMDYEHVMKTLKI
                     LQGELDAIYNKDPKG"
     gene            7936..8589
                     /gene="grpE"
                     /locus_tag="ACZPD4_03750"
     CDS             7936..8589
                     /gene="grpE"
                     /locus_tag="ACZPD4_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1378220.1"
                     /translation="MSKEDMIKEAVEEAKAEAAKMEDEAAADEQDEAAETKGSVEEEA
                     SKETEESQDTKDTESKEKKKFFAKKEKKDKKDEKIEELTDRLTRQMAEFDNFRKRTER
                     EKSQMYEIGAKDIIEKILPVIDNFERGLAAVPEESKEDPFVEGMEKIYKQIMTTLEGV
                     GVKPIEAVGQEFNPDFHNAVMHVEDEEAGENIITEEFQKGYMYHDSVVRHSMVKVAN"
     gene            8649..>9622
                     /locus_tag="ACZPD4_03755"
     CDS             8649..>9622
                     /locus_tag="ACZPD4_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1378221.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGQPTVIANTEGARTTPSVVAFTK
                     TGERLVGEPAKRQAVTNADKTISSIKREMGSDFKVTIDEKKYSPQEISAMILQKLKAD
                     AEGYLGEKVTEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGLDNE
                     KEQKIMVYDLGGGTFDVSIIEIGDGVIEVLSTAGDNRLGGDDFDQKITDYMLADFKAK
                     EGVDLSTDKMALQRLKEAAEKAKKELSSATTTNINLPFITATAEGPKHFDMNLTRAKF
                     DELTRDLVDRTAEPVRRALSDAGLTAADLGQVLLVGGSTRIPAVQEEV"
BASE COUNT         3122 a         1556 c         2569 g         2375 t
ORIGIN      
        1 taagtatatg ggcactgtgg atctttcaca gccgctgaaa gaaaatttag agttggtcgc
       61 agccgatgac aaaaaatgaa aagacgacca ttatgtatat tttgcgtact gtttcttaca
      121 gtacaggcga taagggtgct gtttttcggc gcgggagatt acacttccgc gcttgggcag
      181 atcgtaaacc gaaacaataa aggagaaatc aatcttatat tgacgggaac ggcagatagg
      241 attgatgaaa aagaatctgt aacggcagtc cgtgtaaagg ggaatgccgt ctctttttat
      301 agtggcagcg atcgaaagat cgcaaggcag tctgtattcg gaaaaagcga cgggcatcgt
      361 tcttcctatg atttcagtca agagctgcca agaaaaatat gggaagagaa agtgcttgtt
      421 tatcttgaaa aagaagatgc ggcacatatc aaaatcggaa ataaactaaa aataaaagga
      481 aaggcgtctg catttgagac tgcacgtaat ccgggcaatt ttgatcagag agcttattac
      541 aaaaggcaga aaataggcgc atatgtatgg gcggatgaag tcggtatatt atccgggcag
      601 actgaatgca tcagacaatt cctgcatgaa ttcaggcaag gttggaagaa actccttgta
      661 gggtgtttgg gcagttatta tggaaacacg ataagtgcga tccttctcgg agaaaaagga
      721 gaccttgatc catatatgaa aaagttgtat cagaaaaacg ggatcggtca tctgctggcc
      781 atatcagggc ttcatatgtc ctttatcgga agcggtcttt atggatttct gagaaaaaga
      841 ggagtgggat ttccggtgtc aggagctgtc ggaacgattg ttttgctttt ttatacagta
      901 atgaccggag cgagtatttc agctttaaga gcgcttatta tgttttccgt tcggatgggc
      961 gctgaagtga caggaagaga ttgtgacctt cccacaagtc ttgctgtggc agcggcgctg
     1021 cttagtgcaa aagatcctct ctgtctgtca gatgcaggat ttttgttttc ttttggatca
     1081 ctttgcggaa tttgtatgct gacgcctttg tttgaatgtt tgttcgggtg tggtacggag
     1141 aaaaagaata tatggacgca tatggggaaa gcggtgagcg caagtttggc agtgaatctg
     1201 tttttgcttg gtcctatgct gtacttttat tttgaaatcc cgccgtactc ggttttttta
     1261 aatttgtttg tgatcccggc tctcccggtt attatgggaa cggggctgtc gggttctgtg
     1321 atgatcctgc ttgtaaaacc gatcggtatt attttgctga aaagttccgg cgtgatactt
     1381 tgggcgtatg atcgattgtg ctctgcggca gttatgcttc cgttcgggcg gataacggca
     1441 ggaaaaccga gcgtacaatt acttgtcgct tattatgtga tattgttcgc gttatatgga
     1501 atgtatcggc gttttccaaa gaaaatatgg gggtatatgc tttttgtttc ggcggcgttt
     1561 ttgattgttt tatgcggagc aaaaacccgg gatattgaag gaataaaaat aacagtgctg
     1621 gatgtggggc aaggtgatgg cattgttctt tcgggaaaag ggaaaaacta tttgatagac
     1681 gggggcagca gtgatgtaaa acaggcaggc gctcaacgga tcgaaccgta tttgttgtcg
     1741 gagggcatcg gatgccttga ttatgtattt gtgactcacg gagatgagga tcatatcagt
     1801 ggaataagag aacttctgga agggcagaag ctgggagttc gtattgacac gcttgtgctt
     1861 cctcccgaag aatatcatga tgaaaagttg gcagatctgg cgcgcatagc ggtagaaaac
     1921 ggaacgcgtg tcgtttctgt gaaaacagga gcaaatatat atggaagagg agaaaggcag
     1981 acagaaaaaa acgattcaaa agaagtgatg cggttgagat gtatcggccc gcttacggac
     2041 ataccgcagg ggctgacaaa accaaaggcg ggaaatgagg cgtctcttgt gcttgagttt
     2101 tcttatggga atttcgatat gctttttacc ggggatgtgg aaggtgcggg agaggaactt
     2161 cttgttaaaa gcgatgtttt gagaaaatat gaaattttaa aatgtgccca tcatggatcg
     2221 aaaaactcgg gtacagctgc atttcttgag aagacagatc caagagcggc tatcatctcg
     2281 gcaggaattg ataatcggta cggacatccg catgaagaga cgttaaatag gttgaaaaag
     2341 agaaaagtga aaacatataa tactcagaca gacggggctg tcacaataaa gtctgacgga
     2401 atacagatta gtattgagag ttttctcttg tcaaaatgat tgtttttcct ttataataca
     2461 agagttatga aaagcttaaa tgaagatttg aaaacgggac aatttaaaca gatttatctt
     2521 ctgtacggag aagagtcata tttgaagcga cagtataggg aacggcttgt cagagcgatg
     2581 attccggaag gcgatacgat gaattatgcc gtctatgaag gaaagaacat agatataaaa
     2641 gaggtaatcg atctggcgga gacattgccg ttttttgcat cgcgccgtct tattgtgttt
     2701 gaagatacag gcttttttaa aaatgcagga acagatctgg cagattatat tacggacatc
     2761 ccgcctacta cttatttttt atttatagaa aaagaggtag ataagcgaaa caggctgtat
     2821 aaagcggtaa aggcaaaagg tcatattgtc gagttgacgg ttcaggatga aaatacgctg
     2881 cggcgctggg tgcttggtct tgtaaagaaa gaaaaaaagc agatggaagg cgcagatgcg
     2941 gcatattttc tgaataaggt cggcacggat atggagaata tcacacaaga actggaaaaa
     3001 ctgttttgtt atacgatcgg aagagatcgg attacaagag acgatattga tcagatctgt
     3061 gtcactcaga ttacgaacca tatatttgag atggtcaatg cggttgcagg aaaagaccag
     3121 aagagggcgt tggatcttta ttatgagcta cttgccctga aagaaccgcc gatgcgtatt
     3181 ctgtttttga tgacgagaga ataccgcctt ttatttcatg tcaaaggtct gatggtgaat
     3241 ggatacggga agaaggaaat cgcttcaaag gcagggatcc atccgtttgc tgcgggaagg
     3301 tatatggatg cggcgaaacg atatgaactc ggagagctga gaactgttat ggaagaaggg
     3361 gcgcagttag agcaaaatgt taagaccgga cttatgacag atactttggc tgtggaactt
     3421 tttattgtga agaattcggg aaagcagtga agtttatcac aaaatcaaat acagaaatca
     3481 tataaaatat gcaaataatg gaggaatgaa attgtcagtt atagatcaga gtaaaataag
     3541 aaatttttgt attatcgcgc atatcgatca cggaaaatct acgttagcag acaggattat
     3601 tgaacagaca ggcctcttga cgagcagaga gatgcagtct caggtgcttg acaacatgga
     3661 gcttgaaaga gaacgcggaa ttacgattaa agcacagacg gttcgtaccg tatataaggc
     3721 agacgacggg gaggaatata tgtttaacct gatcgatacg ccgggacatg tagactttaa
     3781 ttacgaagtt tcaagaagtc tggctgcgtg tgacggagcg attttagtag ttgacgcagc
     3841 tcagggagta gaagcacaga cattggcgaa tgtatatctt gcgttggatc atgaccttga
     3901 tgtgatcccg gtgatcaata agatcgatct tccgagtgca gatccggaga gggtgaaaga
     3961 agaaattgag gatgtgatcg gtattgaagc ggaagatgcc ccgcttattt cagctaagac
     4021 aggacaaaat atccatgacg tattggagca gatcgtgaaa aaagttcctg cgcctcaggg
     4081 agatccaaag gctccgttaa aagcgctgat ctttgactcc gtctatgatt cttacaaagg
     4141 ggtcattgta ttttgcagga taaaggaagg aacggtgaag aaaggaactc cgatgcttat
     4201 gatggcgaca ggcgctcagg cagatgttgt ggaagtcgga actttcggag cgggaagatt
     4261 tataccatgt gaagagttga gcgccggaat ggtcggatat attactgcca gcctgaagaa
     4321 tgtaaaagag acgcgggtgg gcgatacagt gactgatgcg tcaagaccgt gtgactcgcc
     4381 tttgccggga tataaaaaag taaatccgat ggtatactgc ggactttatc cggcagacgg
     4441 agcaaagtat ccggatcttc gagacgctct tgaaaaatta cagctcaacg atgcggcgct
     4501 gcaatttgaa gcggaaacat cggtcgctct tgggtttgga ttccgctgcg gatttctcgg
     4561 acttcttcac cttgagatta tccaggaacg tttggagagg gaatataatt tggatctcgt
     4621 aacaacggcg ccgagtgtaa tttacaaaat ccataaaaca aacggagaag tgatcgatct
     4681 gacaaatccg acgaatatgc cggatccggc ggagattgat tatatggaag aacctatggt
     4741 aaaagcggag atcatggtta catcggaata tatcggtgcg atcatggatc tttgtcagga
     4801 acgcagaggc atttatcagg gaatggaata tatcgaggaa aaacgtgccg tattgaatta
     4861 tcatcttccg ctgaatgaaa tcatctatga ctttttcgat gcattgaagt caagatcaag
     4921 aggatatgcg tcatttgatt atgagatgat gggatatcag aaatctgaac ttgtgaagct
     4981 tgatattctt attaataaag aagaagtgga tgcattgtcg tttattgtat ttgcaggaag
     5041 cgcttatgac cgcggaagaa aaatgtgcgc gaaattaaaa gaagaaattc cgagacagct
     5101 ttttgagatt ccgatacagg cggcagtcgg aagtaagatc atcgctcgtg aaacagtaaa
     5161 ggcaatgaga aaagatgtgt tggcaaaatg ttatggcggt gatatttcac gtaagagaaa
     5221 actgcttgaa aagcagaaag agggaaagaa aagaatgcgt caggtcggca gcgtagagat
     5281 accgcagaaa gcatttatga gcgtactgaa gttggatgac gagtgatttt tgttgtcgat
     5341 gtataagtga aaaagtgatg tttaaaacat cttaaagagg taaaggaaaa tatgaaaaaa
     5401 gaactggaac tgtatgtgca tattcctttt tgcgtaaaga aatgtgcgta ttgtgatttc
     5461 ctttcgtttt ctgcaaagca agaggaggtt tctgcttatg tggaagctct tgcggaggag
     5521 ataaaaggaa aaaaagaaca gttttcggat tattgcgtga caacgatttt tttaggcggt
     5581 ggaactcctt ctatattaga gggagtatac actgcctcta tttttcgtgc tttgcgggaa
     5641 agtttcgata ttgcggagaa tgcggaaata acgatggaag taaatcccgg aactgtgtcg
     5701 gaagaaaaga taaacatgtg gaaaacatgc ggagtgaacc ggttaagtat cggtcttcaa
     5761 tcggtcgatg acggagaact gaaaatgctt ggacgtatcc atacatatga tgaatttctc
     5821 actacatgga agatggtaag aaaagcagga tttcataacg ttaatatcga tttgatctcg
     5881 gcgattcccg ggcagacaaa aaagagctgg gaaacaacgc ttcgcacagt tgcgcagtta
     5941 cagccggaac atatttccgc atatagttta attatagaag aaggaactgt tttcggtacg
     6001 atttacgggg aggatgggga gcggatatgc caaaaagaac tgttgtcccg cgagagtagt
     6061 atgccggaag tatcagataa agacggtaat ataagaaaag acagacagat acttccgctt
     6121 cccgatgaag atacagaacg ggcgatttat gaaataacag agaagatcct tcgggaatac
     6181 gggtatgaga gatatgagat ttcaaattat gcaaaaccgg gatatgagtg caggcataat
     6241 ttagggtatt gggagcgaag agaatatctc ggactgggac tgggggcgtc ctctctgatt
     6301 gccgaacacc gattctgcaa tacagataaa atggatgttt atataagact gaataagact
     6361 gtagatccgg acggcaaaaa agaagagagt aaaaaagcgg tatgggaaaa agcagaagaa
     6421 gtaagtgtac tgtcagtgca agagcaaatg gaagaattca tgtttctcgg actgcggaaa
     6481 acagatggag tgtcggaaaa aagttttttt gaaacattcg gggtgcaaat agatacggtt
     6541 tacggagaaa cgatcggaaa acttgaaaaa gaacgtcttt tgataaaaga aaaagggcag
     6601 atccgcctga cagagcgggg agttgatata agcaactatg taatgagtga gtttttgttt
     6661 tgaaaaagcg gatgctgtga ttctgcctct ttcggataat aggaaattca gatagaagac
     6721 atcgtttcgg atgtacagaa ataaataaaa aaataataaa cactgttgac aaaaggaaaa
     6781 tgcagtgcta tcttaatata cgaaaggtgt tagcactcga aagaaaagag tgctaatagc
     6841 gagagaggag gaaaagctgt ggcggtcgat acgtcgttaa gtgaaagaaa acttactata
     6901 ttaaaagcga tcatacagaa ttaccttgag accggagaac cggttggctc gcggacactt
     6961 tctaaatata cagatctgaa tttaagttcc gcgacgatca ggaacgagat ggcagatctt
     7021 gaagatctcg gttatatttt tcagccgcat acgtcggcgg gaaggattcc ttcggataaa
     7081 ggatatcggc tttatgttga tatgctgatg gaagaaaaag aacaagagat tgcaaaaaga
     7141 gaaagcgcga tgttggagaa aaccgataag gttgagaaag tattacagca ggcagcaaga
     7201 gttttggcga cgaatacgaa ttatgcgacg atggtatcgt ctccgattaa caatcggaat
     7261 acgctgaaat ttattcagct ttctcaagtt gatgcagaac agatcgtagc ggttatcgta
     7321 cttggcggta atgtaatcaa gaataagatc atcgaagtag gggaaatgtt aagtaatgag
     7381 aatcttctga agttaaatat gcttcttaat actacattga acggacttcc gatcgatcag
     7441 atcacacttg gattgatcgc aaggctgaaa gagcaggcag gcattcacag tgaggttgtc
     7501 gctcatgtac tggatgcggt ggcagagacg atccatgtag aagaagatat gaagatttat
     7561 acaagcggag ctacgaacat ctttaagtat ccggagttga gcgataagca aagcgcacag
     7621 gagatcatca gtgcatttga agagaagcag cagctcgccg atcttgtgac agagacactg
     7681 gcaagtgagg atagtcacgg aatacaggtt tatatcggag atgagactcc ggttaaaaca
     7741 atgaaggact gcagtgtcgt gacagcgacg tatgaacttg gcgaaggcat gaaaggaacg
     7801 atcggcatta tcggaccgaa acggatggat tatgaacatg tcatgaagac gctgaaaatt
     7861 ctgcagggag agttggatgc gatttataat aaagatccaa aaggatagaa aggtggaaag
     7921 ccggtgggtg acaagatgag caaagaagat atgataaaag aagctgtcga ggaagcaaag
     7981 gcagaagcgg caaagatgga agacgaagcg gctgcagatg agcaggacga ggcggcagag
     8041 acgaaaggat ctgttgagga ggaagcttca aaagagacgg aagagtctca ggatacaaaa
     8101 gatacagaat caaaagaaaa gaaaaagttt ttcgcaaaga aagaaaaaaa agataaaaaa
     8161 gacgagaaga ttgaagaatt gactgatcgt ctcacccgtc aaatggcaga gtttgataac
     8221 ttccgcaagc gtacagagag agaaaaatct caaatgtatg aaatcggagc gaaagatatc
     8281 atagaaaaaa ttcttccggt tatcgataat tttgaacgtg gtcttgcagc cgtgccggag
     8341 gaaagtaagg aagatccttt tgtagaagga atggaaaaaa tctacaaaca gatcatgacg
     8401 actttggaag gggtcggtgt aaaaccgatc gaagcggtag gccaggaatt taatcctgat
     8461 ttccataatg cggttatgca tgtggaagat gaggaagcgg gtgaaaacat catcacggaa
     8521 gaatttcaaa aaggctatat gtatcatgac agcgtcgtaa gacacagtat ggtaaaagta
     8581 gcaaattagt agaaaagcaa ctttatttca gaataataaa aagcgataag aagcaggagg
     8641 aaaaaatcat gggaaaaata atcggtattg accttggtac aacaaacagt tgcgtagctg
     8701 ttatggaagg cggacagccg acagtgatcg caaacacaga aggcgcaaga acaacgccgt
     8761 cagtcgtagc gtttacaaag acaggagagc gtctcgtagg agagccggca aaacgtcagg
     8821 cagttacaaa cgcagacaaa acaatttctt ctatcaaaag agagatggga agcgatttta
     8881 aagtgacgat cgatgagaaa aaatattctc ctcaggaaat ttccgcaatg atccttcaga
     8941 aattgaaagc ggatgcagaa ggttatttgg gagaaaaagt aacggaagct gttattacag
     9001 ttccggcata cttcaacgac gcgcagcgtc aggctacaaa agatgcagga aagatcgcag
     9061 gtcttgatgt aaaacgtatc atcaacgagc ctacggcagc ggctcttgct tacggcctgg
     9121 acaatgagaa agagcagaaa atcatggtat acgaccttgg aggtggtacg tttgacgttt
     9181 ccattatcga gatcggcgac ggcgtgatcg aagttctctc aacagcagga gataaccgtc
     9241 ttggcggtga tgactttgac cagaagatta cagattatat gctggcagat tttaaggcaa
     9301 aagaaggcgt tgacctttca acggataaga tggctcttca aagattaaaa gaagcggcag
     9361 agaaagcaaa aaaagaactt tcttccgcaa caacaacaaa tatcaacctt ccgttcatta
     9421 cggcaactgc cgaaggaccg aaacactttg atatgaatct gacaagagca aaattcgatg
     9481 agctgacaag agatctcgta gacagaacgg cagagccggt aagacgcgct ctttccgatg
     9541 cgggacttac agcggcagat ctcggtcagg tattgcttgt aggcggatct acacgtattc
     9601 cggcagtaca ggaagaagta aa
//
LOCUS       DBMDXF010000059         9577 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1953_length_9577_cov_2.172963, whole genome shotgun sequence.
ACCESSION   DBMDXF010000059 DBMDXF010000000
VERSION     DBMDXF010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1953_length_9577_cov_2.172963"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(23..1471)
                     /locus_tag="ACZPD4_03760"
     CDS             complement(23..1471)
                     /locus_tag="ACZPD4_03760"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1378222.1"
                     /translation="MSAQNLTLLTDLYELTMMQGYFESQANETVIFDVFFRENPNKGG
                     YSVMAGLEQVIEYIKNLNFSYEDVDYLRGLGLFSEDFLHYLSGFHFSGDIYAIPEGSV
                     IFPREPLVKVIAPIMEAQLVETAILNIINHQSLIATKASRVVHAAQGNGVMEFGLRRA
                     QGPDAGLYGARAAMIGGCVGTSNVLAGKMFDVPVLGTHAHSWIMSFPDEYTAFKEYAR
                     LYPDACTLLVDTYDTLKSGVPNAIRVFQKMKDAGIHPKSYGIRLDSGDLAYLSKKARK
                     MLDEAGFPDAVIAASNDLDEFLINDLKIQGAAITSWGVGTNLITSKDCPSFGGVYKLA
                     AIQNKDGEFVPKIKISENIEKITNPGNKTIYRIYDKDTGKIRADLICFADETYDTSED
                     LLLFDPNSTWKKTRLEGGTYTMKEILKPIFIRGECKYTSPSVKEIASFCEQEKNTLWD
                     ETKRLFNPHKVYVDLSQRLYDTKTELLNNVNR"
     gene            complement(1615..3036)
                     /gene="hydG"
                     /locus_tag="ACZPD4_03765"
     CDS             complement(1615..3036)
                     /gene="hydG"
                     /locus_tag="ACZPD4_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HIR1378223.1"
                     /translation="MYDVNSKHADDFINHEEILETLAYADENKNNMKLIDAIIEKAKK
                     RKGLTHREASVLLACSDEEKNKEIYKLAEQIKKDFYGNRIVMFAPLYLSNYCVNGCTY
                     CPYHAKNKHIMRKQLSQEEIRREVIALQDMGHKRLALEAGEDPVRNTMDYYLESIKTI
                     YSIKHKNGAIRRVNINIAATTVDNYRLLKEAGIGTYILFQETYHKESYLELHPTGPKH
                     DYNYHTEAMDRAMEGGIDDVGLGVLFGLDKYRYEFAGLLMHAEHLEAAFGVGPHTISV
                     PRLKHADDIDPDQFDNGIDDDTFAKIVACIRIAVPYTGMIISTRENQKTRERVLHLGI
                     SQISGGSRTSVGGYCEPEPEDAKSEQFDVSDTRTLDEVVRWLMEMDYIPSFCTACYRE
                     GRTGDRFMSLCKSGQIQNCCHPNALMTLKEYLMDYASPETKAIGDRLIDREVLNVPNE
                     KARAVVLENLKLIEQDNRRDFRF"
     gene            complement(3134..4192)
                     /gene="hydE"
                     /locus_tag="ACZPD4_03770"
     CDS             complement(3134..4192)
                     /gene="hydE"
                     /locus_tag="ACZPD4_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HIR1378224.1"
                     /translation="MTEKNDTKQLIDRLEATRNLSRDEWIRLIQNRTADDADYLFERA
                     QAVRTRHYGRSVYIRGLIEFTNYCKNDCFYCGIRKSNRNANRYRLTKEQILSCCEMGY
                     ALGFRTFVLQGGEDGSFTDDRLTDIISSIRTRWSDCAITLSIGERSYESYKQLYDAGA
                     NRFLLRHETYDDTHYGKLHPPALSAARRKQCLFDLKKIGYQVGTGFMVGSPYQTFENL
                     ADDMLFLKELNPQMVGIGPFIPHHDTPFADQKAGSADLTLFLLGLIRLLLPKVLLPAT
                     TALGTIDSDGREKGILAGANVIMPNLSPPQVREKYLLYDSKLCTGDEAAESLRSLKQR
                     IQNIGYEITVSRGDSLNI"
     gene            complement(4332..4580)
                     /locus_tag="ACZPD4_03775"
     CDS             complement(4332..4580)
                     /locus_tag="ACZPD4_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HIR1378225.1"
                     /translation="MDTRIALIGVLLETRESVDKLNHLLSDYGEYVIGRMGLPYKEKG
                     IHIISIAVDAPNNIISALSGKLGMLPGVSTKTIYGKTT"
     gene            5043..5315
                     /locus_tag="ACZPD4_03780"
     CDS             5043..5315
                     /locus_tag="ACZPD4_03780"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR1378226.1"
                     /translation="MAEIRKRIVFHGRVQGVGFRYTAKYLAQSLELTGWVRNDWDQTV
                     TMEVQGREALINKLLVGLNQGRFIDIQWMDTEEIPLEEEREFTVKY"
     gene            5423..6217
                     /gene="proC"
                     /locus_tag="ACZPD4_03785"
     CDS             5423..6217
                     /gene="proC"
                     /locus_tag="ACZPD4_03785"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1378227.1"
                     /translation="MKLGFIGTGNMAGAIMGGIIRNEIFKPEEIIGADISKESRSKVQ
                     SAYGINVTEDNKKAASESEVLVLSVKPQYYAETIKEIKDCVREDQLIITIAPGKTLKW
                     LEEQFEKKVKIVRAMPNTPALVGEGMTAACANDAVTEEEKEYALKILRSFGKVEIVAE
                     NMIDAVVAVSGSAPAYVFMFIEAMADAAVSEGMPRKQAYEFAAQAVYGSAKMVLDTGK
                     HPGELKDMVCSPAGTTIEAVRVLEEKGLRSAVIEAMKVCADKSRKM"
     gene            6338..7252
                     /gene="trxB"
                     /locus_tag="ACZPD4_03790"
     CDS             6338..7252
                     /gene="trxB"
                     /locus_tag="ACZPD4_03790"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR1378228.1"
                     /translation="MYDIGIIGGGTAGMTAAIYGQRAGKRTIIIEGGTFGGQITSSPN
                     VENYPGIASVSGSEFSMNLLDQAMKLGAETQMAQVTGIRDEGEVKVIVTPEKEYPCRS
                     IVLATGVTHRHLGVPNEERLTGAGVSYCATCDGMFFRGKEVAVVGGGNTAIQDAEFLS
                     DYCSKVYLIHRRDEFRGENSGVKRLKEKENVEFILSATVKELVGESMVEKLILNDKKT
                     GKDFELHVSGVFVAVGQIPKNETFADTVKLDEGGFVLASEDCLTSHPGIFAAGDCRTK
                     EVRQLTTAAADGAVAALAACKYIADQSL"
     gene            complement(7377..8114)
                     /locus_tag="ACZPD4_03795"
     CDS             complement(7377..8114)
                     /locus_tag="ACZPD4_03795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1378229.1"
                     /translation="MFRFICIVIFLILFLILTIPILIVEWIIGKFAPNARDISSLRIV
                     QWGFKVILKITGVKTTVIGEENIPDEAVLFVGNHRSYFDILLTYSRCKRLTGYVAKKE
                     MEKYLTLTTWMRRLYCLFLDRSDPKQGLKTILTAIDYIKRGISICIFPEGTRNTGAEL
                     SLLPFKDGAFKIATKTGCPIVPICMNNTAEIFENHFPKIRKTHVVIEYQKPIYPDRLD
                     KETKRHIGDHVESIIKETIEKNAKLYF"
     gene            8245..8733
                     /locus_tag="ACZPD4_03800"
     CDS             8245..8733
                     /locus_tag="ACZPD4_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR1378230.1"
                     /translation="MEKCRGSIGMDRGYAKRKLLKKDTQKAAVFGAVLICAAAFTAAL
                     TGCVSTQAETEKIRDMKYEVIEEEDIPQSLREMIKGGEKKPFRITYSDQGFLYIAQGY
                     EVQPTTGYSVEVKELYETETAVCIKTTLIGPKKGEEKEKAETYPYIVVQTEDVKKDVL
                     FK"
     gene            8745..9320
                     /locus_tag="ACZPD4_03805"
     CDS             8745..9320
                     /locus_tag="ACZPD4_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HIR1378231.1"
                     /translation="MDLTKKTVHYTQEGKTVFDRFYLDEDYNVPEQKEAVQRIVYSSA
                     KLKTEDVRPVENYVKVTGKAYYKILYMTQGETTLSVLEGKIPFEEMIYAENDGEETFF
                     IRNERTEFTVSVVHSRKLTLRVAAEMELGRERMRDEELTTDAESDFPVYKKRQKMNVS
                     ELKISKKRYVPYQRRNSTAGNERKYRTDFIF"
     gene            9343..>9577
                     /locus_tag="ACZPD4_03810"
     CDS             9343..>9577
                     /locus_tag="ACZPD4_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HIR1378232.1"
                     /translation="MRPGQDELLIRGEAEVFCMYLSPEEKVEWIEQSVPYEGRIPCEG
                     AEEDMICQIRQSLEDPIVDIRQDGDGEMRALGIE"
BASE COUNT         2717 a         1807 c         2401 g         2652 t
ORIGIN      
        1 tagattcata agagagttgt tcctatcggt ttacgttatt taacagttcg gtttttgtat
       61 cataaagtct ttgtgaaagg tctacatata ctttgtgcgg gttaaagaga cgctttgtct
      121 catcccacag agtatttttc tcctgttcgc agaaagaggc aatctctttt acggacggag
      181 aagtgtattt acattcgcca cggataaaga tcggttttaa aatctctttc attgtatatg
      241 tgccgccttc aagccgtgtc tttttccatg tggaattcgg atcaaaaaga agcagatctt
      301 cagaagtatc gtaagtttca tctgcaaaac agattagatc ggcgcggatt tttcctgtat
      361 ctttatcata aatgcgatag atcgttttgt ttccgggatt ggtgattttt tctatatttt
      421 cggaaatctt gattttcgga acaaattccc cgtctttatt ttggatggcg gcaagtttgt
      481 aaacccctcc gaatgacgga cagtcttttg atgtgatcag attcgttccg acaccccatg
      541 aagtgatggc agctccctga attttcagat cgttgatcaa aaattcgtcc agatcatttg
      601 acgcggcgat aactgcgtca ggaaatccgg cttcatcgag cattttccgt gcctttttgg
      661 aaaggtaggc aagatcgccg gaatcaagac ggattccgta acttttcgga tggattccgg
      721 catctttcat tttctgaaat acacggatcg cattcggaac acctgatttt aatgtgtcgt
      781 atgtgtctac gagaagcgta caggcgtcag gatacagtct ggcgtattct ttaaatgccg
      841 tatattcgtc aggaaaactc atgatccagc tgtgtgcatg tgttccgaga acaggaacgt
      901 caaacatttt tcccgcgaga acattggacg ttccgacaca acctccgatc atggctgccc
      961 ttgccccgta aagacctgca tcaggtccct gggcgcggcg cagaccgaat tccatcacgc
     1021 cgtttccctg tgccgcgtgg acaacgcggg aagcctttgt tgcgatcaaa gactgatggt
     1081 tgataatgtt taagatcgcg gtttcgacta actgcgcttc catgataggg gcgatcactt
     1141 tcacgagcgg ttcccgcgga aagatgacgc tgccttcagg aatggcataa atatcgccgc
     1201 tgaagtggaa tccgctcaag tagtggagga agtcctcgct gaacagtccg agtcccctta
     1261 gataatctac gtcttcataa gaaaaattca agtttttaat atattcgatc acctgctcta
     1321 aaccggccat gacagagtat ccgcccttgt ttggattttc gcggaaaaac acgtcgaaaa
     1381 tgaccgtttc gtttgcctgc gattcaaaat atccctgcat cattgtcagt tcgtaaagat
     1441 ctgtcagcaa ggtgagattt tgtgcgctca tggttattcc ctcttttcat tgccatgcat
     1501 aagacgaaac gatgaaactg ctttcgtctg tatcgtaaat ttgcagagga agcagaagtg
     1561 gtttttactg cggcttccgt ctgccaacgc tcattatatc acaagaaaat aaatttagaa
     1621 acgaaaatct cttctgttat cctgctcgat cagtttcaga ttttccaaaa cgacggcacg
     1681 cgctttctca ttcggaacat tgagaacttc tctgtcgatc agccggtctc cgattgcctt
     1741 tgtctcagga gacgcataat ccatcaaata ttctttcagt gtcatgagag cgttcggatg
     1801 acagcagttt tggatctgtc cgcttttgca aagagacata aaccgatctc cggttcttcc
     1861 ttcacgataa catgcggtac aaaaacttgg gatgtaatcc atttccataa gccagcggac
     1921 aacttcgtca agtgtgcggg tatcgctgac gtcaaactgt tcggatttgg catcttcagg
     1981 ttccggttca cagtaccctc ctacgctggt tcgtgatccg ccgctgatct gggagattcc
     2041 aagatgaagg acgcgctccc gtgttttctg gttttcgcgg gtagagataa tcattcccgt
     2101 atatggaacg gcgattcgga tgcaggcaac gatcttggca aaagtatcgt cgtcgattcc
     2161 gttgtcaaac tgatcgggat cgatatcgtc ggcgtgtttc agacgaggaa cgctgatcgt
     2221 gtgcggaccg actccgaacg ccgcttccag atgctcggca tgcatgagaa gcccggcaaa
     2281 ttcatagcgg tatttgtcca gtccgaagag aacacccaga ccgacgtcgt ctatgccgcc
     2341 ttccatggcg cggtccatcg cttcggtgtg atagttgtag tcgtgttttg gtccggtcgg
     2401 atgcagttca agatagcttt ctttatggta tgtttcctga aacaagatgt acgttccgat
     2461 gccggcttct ttgagcaggc ggtaattatc gactgtcgta gccgcaatat taatattgac
     2521 acggcggatc gcgccgtttt tatgtttgat gctgtaaatc gttttgatcg attctaagta
     2581 atagtccatc gtattgcgga caggatcttc gcctgcttcc aaagcaagcc gtttatgccc
     2641 catgtcctga agtgcgatga cttctcttcg gatctcttcc tgagagagct gtttcctcat
     2701 aatatgtttg tttttcgcgt ggtacgggca gtaagtacag ccgttgacac aataatttga
     2761 cagataaaga ggagcaaaca ttacgattct gtttccgtag aaatcttttt tgatctgttc
     2821 tgcaagctta tagatctctt tgtttttttc ttcgtccgaa caggcgagaa gaactgatgc
     2881 ttcccgatgt gtcagccctt ttcgtttttt tgctttttca atgatcgcat cgatcagttt
     2941 catgttattt ttattctcat ctgcataggc gagcgtttcg agaatttctt catgattgat
     3001 aaaatcatct gcatgttttg aatttacatc gtacatagtt gagtctcctt ttaattttta
     3061 atattatttt tcggaatttt aaatcgtttt atctgctttc agacagggaa aatgtttcgg
     3121 atgaaagtgc tttttatatg ttcagggaat ctcctctgga aactgtgatt tcatatccga
     3181 tattttgtat tctctgcttt aaagaacgaa ggctttcggc ggcttcgtca ccggtgcaca
     3241 gtttactgtc gtaaagtaaa tatttttctc tcacctgcgg cggagaaaga ttcggcatga
     3301 tcacgttcgc tccggcaaga attccctttt cgcggccgtc ggaatcaatc gtgccaagag
     3361 cggtcgtggc agggagaagg actttcggaa gaagcagacg gatcaatccg agcagaaaca
     3421 gcgttagatc agcgcttccg gccttttgat ctgcaaacgg ggtgtcatga tgggggatga
     3481 aaggaccgat cccgaccatc tgtggattga gttcttttaa aaacagcata tcgtctgcca
     3541 gattttcaaa agtctgataa ggcgatccga ccataaatcc ggtgccgacc tgatagccga
     3601 tcttttttag atcaaaaaga cattgctttc gtctggcggc gcttaaggca ggggggtgaa
     3661 gctttccgta atgcgtgtca tcgtatgttt catgtcttaa aagaaagcga ttcgctccgg
     3721 cgtcataaag ttgcttgtag ctttcataag aacgttctcc gattgagagt gtgatcgcgc
     3781 agtcactcca tcgcgttcgt atagaagaaa taatatcagt cagccggtca tccgtaaatg
     3841 aaccgtcctc accgccttgc agaacgaacg tccgaaagcc gagagcatat cccatctcac
     3901 agcaggagag tatctgctct tttgtcagac ggtaacggtt ggcatttctg ttactttttc
     3961 gtattccgca gtagaagcag tcgtttttac aataatttgt aaattcgatc agtccgcgga
     4021 tgtatacgct gcgtccataa tggcgtgtgc ggacggcctg tgcccgttca aataaatagt
     4081 ctgcatcatc ggccgttctg ttttgaatca ggcggatcca ttcgtctctg gaaagattac
     4141 gggttgcttc taatcggtca atcagttgtt ttgtatcgtt tttttcggtc atagtcgtgt
     4201 cctctcgcag aaaaataagt gtgacagttc agttcgcgcc attcgtaaaa ccgtactgcg
     4261 tgcttatcgc ggctgacgcc gctgttttac tcataagcgg gcgtttggat catatagctg
     4321 ttacaaataa gttacgttgt tttgccgtag atcgttttcg tgctgacgcc cggaagcatt
     4381 ccgagtttgc cggaaagggc gctgatgata ttgttcgggg cgtctacggc gatactgata
     4441 atatggattc ctttttcttt gtatggcagt cccattcttc cgattacata ttctccgtag
     4501 tcgcttagaa gatggtttag tttatcgacg gattctcggg tttcgagaag aacgccgata
     4561 agagcgattc tagtgtccat agtatgtctc cttgttataa ttcgtatagt gtaagcagtt
     4621 gacaaggttt cagacaagtt cggactttcg tttgcagaca ttgtaaaaag cgcgcctgaa
     4681 aaggcgcgcg gatatacaaa gaaagaggga gagtccctct gatattccat atgtgcgcct
     4741 ttttgctcag gaggatgcct ttgcagtcag aacgtatgtc ttgtgtttga gtatagcata
     4801 aagttggaag aatgacacga aaaattttgg gattcacttg ttgttttatg tattatgcat
     4861 aaaactttaa tatgttttta gtgcggtttc tataatatga tttttatgat ttcatttgtg
     4921 atatagtcac tgttaaaaag tttttattgg tttaatatga aactagagta taatagaaaa
     4981 gggttcgtaa attcatttct atgattgaag catatatttt cagaaattga ggaggcggta
     5041 atatggcaga gatcagaaaa cggatcgttt ttcacggacg cgttcaaggc gtcggatttc
     5101 gttatacggc aaaatatctc gcgcagtctt tggaattgac aggctgggtt cgcaacgact
     5161 gggatcaaac tgtgacaatg gaagttcagg gaagagaagc gctgatcaat aaactgcttg
     5221 tcggcttgaa tcaagggcgt ttcatcgata ttcaatggat ggatactgaa gagattccgt
     5281 tagaggaaga acgggaattt acggtgaagt attgaaaaat gagcaaaacg aaaaactgaa
     5341 aaaatgataa aacagagaaa atattgaata gaacgacgac agataaaagg aaaatagaaa
     5401 tatggaaaag gagaataaga ttatgaaatt aggatttatc ggaacaggaa atatggcggg
     5461 cgcgatcatg ggaggcatta tcagaaatga gatctttaag ccggaagaga tcatcggcgc
     5521 ggatatttca aaggaaagcc gaagcaaagt gcaatctgca tatgggatca atgtgacaga
     5581 ggataataag aaagcggcgt cagaatcaga agttcttgta ctctcggtga agccgcagta
     5641 ttatgcggag acgatcaagg agattaaaga ctgtgtaaga gaagatcagc ttatcattac
     5701 gatcgctccc ggaaagacgc ttaagtggct ggaagaacag tttgaaaaaa aggttaagat
     5761 cgttcgtgca atgccgaata caccggcgct tgtaggagag gggatgacag ccgcatgtgc
     5821 aaatgatgcg gtgacagaag aagaaaaaga atatgcgttg aaaatcttac gttctttcgg
     5881 aaaagtagag attgtggcgg aaaatatgat cgatgctgtc gttgccgtaa gcggaagcgc
     5941 tcctgcatat gtatttatgt ttattgaagc gatggcggat gcggctgtgt cggaaggaat
     6001 gccgagaaag caggcgtatg aatttgccgc gcaggcagtt tacggaagtg caaagatggt
     6061 gctcgatacg ggaaaacatc cgggagaatt aaaagatatg gtatgttctc ctgcgggaac
     6121 aacgatagag gctgtccgcg ttctggagga gaaaggactt agaagcgcgg tgatcgaagc
     6181 tatgaaagta tgcgctgata aatcgagaaa gatgtaataa cgaagaaata aagtgctgca
     6241 aaacagactt ttacagaagc aaggaagcga attgaatttt gcagaatgaa agaaataaac
     6301 aggtcgttta gtgacaggaa aaaaggagat gaaaattatg tatgatatcg gaatcatcgg
     6361 cggcgggact gccggaatga cagcggcgat ctatggtcag agagcgggaa aaagaacaat
     6421 tattatagaa ggaggaacat ttggcggtca gatcacttcg tctccgaatg tagaaaatta
     6481 tccggggatc gcaagtgtga gcggaagcga attttccatg aatctgctcg atcaggcaat
     6541 gaaactcggc gcagagacac agatggcgca ggtgacggga attcgtgatg aaggagaagt
     6601 aaaagtaatc gttacaccgg aaaaagaata tccgtgccgg agtattgttt tagcaaccgg
     6661 agtgacgcac agacatcttg gagtaccgaa tgaagagcgg ctgacagggg cgggagtttc
     6721 ctattgtgca acatgtgacg gaatgttttt cagaggaaaa gaagtcgccg tagtaggcgg
     6781 cggaaacaca gcgatccagg atgcggaatt cctttccgac tattgcagta aagtatatct
     6841 gatccataga agagatgagt tccggggtga aaacagcggt gtgaagcggc tgaaagaaaa
     6901 ggaaaacgta gagtttatcc tcagcgcgac agtgaaagaa ctcgtcggag aatccatggt
     6961 ggaaaaatta attttaaatg ataaaaagac gggaaaagat tttgaacttc atgtgtcagg
     7021 tgtatttgtc gcagtcggcc agatccctaa aaacgagacg tttgcagata cggtaaaatt
     7081 agatgaggga ggattcgttc tcgcatcgga agactgcctt acatctcatc cgggaatttt
     7141 tgcggcggga gactgccgta caaaagaagt aagacagctt acgacggcgg cggcagacgg
     7201 cgcggtggcg gcgcttgcgg catgtaaata tatcgcagat caaagtttgt gagattttgt
     7261 gctgaattat cattttaata agtaaaataa aaaacagttc cgttttacag acagttcgat
     7321 gagtctgtat gaaaagcgga actgcttttt tatttttttg atatttttat ttgagtttaa
     7381 aaatataatt ttgcattttt ttcaatcgtt tctttgatga tcgattctac gtgatctccg
     7441 atatgacgtt ttgtttcttt atcaagccgg tccggataga tcggtttttg gtattcgatc
     7501 acgacatggg tttttctaat cttcggaaaa tgattttcaa aaatttcagc cgtgttgttc
     7561 atgcagatcg gaacgatcgg acagcctgtt tttgtggcga ttttaaaagc gccgtcttta
     7621 aacggaagaa gggaaagctc cgctcctgtg ttgcgcgtcc cttcgggaaa gatgcagatt
     7681 gaaatccccc gtttaatgta atctatagca gtcagtatag tctttagtcc ttgttttgga
     7741 tcggaacggt caaggaagag acaatacagg cgacgcatcc acgttgtaag tgtcagatat
     7801 ttctccattt ctttttttgc gacatatccg gtgagacgtt tgcatcggct gtatgtcagg
     7861 agaatgtcga agtagcttcg atgatttcct acaaagagaa ccgcttcgtc gggaatgttt
     7921 tcctctccga tgaccgtcgt ttttactccg gttattttta aaatcacttt gaatccccat
     7981 tgtacgatgc gcaaagaact tatgtcgcgg gcgttcgggg caaattttcc gatgatccat
     8041 tcaacgatga ggatcggaat tgtaaggatg agaaataata taagaaaaat tacaatacaa
     8101 ataaaacgaa acataaataa acctcatttt cttcttaaat ttgcaataca acggtaatta
     8161 tacagcggag gataggaaaa cacaagactt ataaaaggat agaatgatat aggcataagg
     8221 aaggtgtaca gcgcatatgt ttgtatggaa aaatgcagag gttcgatcgg aatggaccga
     8281 ggatatgcaa aaagaaagct gttaaaaaaa gatacgcaaa aggcggcagt gttcggagcg
     8341 gttctgatct gtgccgcagc gtttacggca gcgctgacag gatgtgtttc aacacaggca
     8401 gagacggaaa agatacgaga tatgaagtac gaggtgattg aggaagaaga tattccccaa
     8461 agtcttcggg agatgatcaa aggaggagag aagaaaccgt ttcggattac atattcggat
     8521 cagggattcc tctatatcgc acagggatac gaggttcagc cgacaaccgg ttatagtgtg
     8581 gaagtgaagg aactttatga gacggaaacg gccgtctgta taaaaacgac tctgatcggt
     8641 ccgaaaaaag gggaggaaaa agaaaaagca gaaacatacc cctatatagt cgtgcagacg
     8701 gaagatgtaa aaaaagatgt tttgtttaag tgaggaggag aaagatggat ctgacgaaaa
     8761 aaacagttca ttatacacag gagggaaaaa ccgtttttga ccggttttat ctggatgaag
     8821 attacaatgt gcccgagcag aaagaagctg ttcaaagaat cgtgtacagc agtgccaagc
     8881 ttaagacaga ggatgttcgt cctgtggaaa actatgtgaa agtaacagga aaagcgtatt
     8941 acaaaatcct ttatatgacg cagggggaga caacgctttc ggtgttggaa ggaaagattc
     9001 cgtttgaaga gatgatctat gcggaaaatg acggcgaaga gacgtttttt atccgaaatg
     9061 aaaggacgga atttactgta tcggtcgttc attcaagaaa actgactctt cgggtagctg
     9121 cggaaatgga actgggaagg gaacggatga gagatgaaga attgacgaca gatgcagaaa
     9181 gtgattttcc ggtatacaaa aaacgacaga agatgaatgt gtcggaatta aagatttcaa
     9241 aaaaaagata cgtaccgtat caaagaagaa atagtactgc cgggaacgaa agaaagtatc
     9301 ggacagattt tattttttga cataagcgga agaaaagcgg aaatccgccc cggacaggat
     9361 gaactgctta tacggggcga ggcggaagta ttctgcatgt atctgtcacc ggaagaaaaa
     9421 gtggaatgga ttgaacagag cgtgccgtat gagggaagga ttccatgcga aggcgcagag
     9481 gaagatatga tctgtcagat ccggcaaagt ctggaagatc ctatcgtcga tatccgtcag
     9541 gatggagacg gagaaatgag agctcttggt atcgaag
//
LOCUS       DBMDXF010000060         9470 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1977_length_9470_cov_1.561746, whole genome shotgun sequence.
ACCESSION   DBMDXF010000060 DBMDXF010000000
VERSION     DBMDXF010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1977_length_9470_cov_1.561746"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            73..2724
                     /gene="mutS"
                     /locus_tag="ACZPD4_03815"
     CDS             73..2724
                     /gene="mutS"
                     /locus_tag="ACZPD4_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1378233.1"
                     /translation="MAELTPMMKQYMETKSQYQDCILFYRLGDFYEMFFEDALTASRE
                     LEITLTGKNCGQEEKAPMCGVPYHAVEGYLNRLVAKGYKVAICEQVEDPKTTKGIVKR
                     EVVRIVTPGTNLDTQALDETKNNYIMCIVYIADRYGVSVADISTGDYFVTEIPDSAKL
                     LDEIYRFSPSEIICNEAFYMSGVDMDGMKDRLGITIYSLESWYFDDEVCRKKLLEHFE
                     VSSFAGLGLADYDCGIISAGALLQYLLETQKNSLSNLTHITPYAAGKFMMIDSSTRRN
                     LELCETLREKQKRGSLLWVLDKTKTAMGARTLRKYVEQPLIDKTEIIRRLDAVQELKE
                     QAISREEIREYLSPVYDLERLITKIAYGSANPRDLTAFRSSLEMLPALLYILQEMKAE
                     LLKDLAVDLDPLEDLCILVKKAIREDPPIAMKEGNIINDGYNEEVDKLRRAKSDGKDW
                     LAKLENDEREKTGIKNLKIKYNKVFGYYLEVTNSYKEMVPEYYTRKQTLANAERYITP
                     ELKELEDMILGAEDKLYALEYELYSEVRDLIASQIERIQKTAKAVAALDAFASLALVA
                     ERNNYVRPKINEKGVIDIKEGRHPVVERMIPNEMFISNDTYLDDKKHRISIITGPNMA
                     GKSTYMRQTALIALMAQIGSFVPAKSANIGLSDRIFTRVGASDDLASGQSTFMVEMTE
                     VANILRNATSKSLLILDEIGRGTSTFDGLSIAWAVIEYISDSRLLGAKTLFATHYHEL
                     TELEGKIDNVNNYCIAVKEKGDDIVFLRKIVKGGADKSYGIQVAKLAGVPELVIGRAK
                     EIVEELSDEDITARVSEIASKERVVKKKPKVKKYDDVDIAQMSLFDTVKDDDVLEELK
                     NLDVGNMTPIDALNTIYRLQNKLKNRW"
     gene            2757..>4735
                     /gene="mutL"
                     /locus_tag="ACZPD4_03820"
     CDS             2757..>4735
                     /gene="mutL"
                     /locus_tag="ACZPD4_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1378234.1"
                     /translation="MPHIQVLDQITIDKIAAGEVIERPASIVKELVENAIDAKATSVT
                     VEIKDGGISFIRVTDNGCGIEADEVRCAFLRHSTSKIRTAEDLVSIHSLGFRGEALSS
                     IAAVTRTEVITKTEEGQSGIKYVIEGGKETALEETGAPNGTTFLIHQLFYNIPARRKF
                     LKTPVTEAGHVQDLLIHLALSHPEVSFLFINNGQEKLRTSGNGKLKDVIYNVYGREVT
                     SNLLELDYKKDGLSISGFLGKPVITRGNRNFENFFVNGRYVKSAMLSKSLEDAYKDFT
                     MQHKFPFAVLHFQVEGDLIDVNVHPTKMELRFQRQQEVYNTMYEAVHRRLLEPELIQT
                     ATVPDPADAAQKKKESPFLLKPKSIENTKYDPIEKIREKMQEREPEQVREKAQETKPE
                     QVREKVQDEEQGQARKQTSAIEADKESVLQSEPQIHDEDYFIRKMRERVKAYHERSSS
                     AEVLDRGEIYRPQQQAERIREAAAYRAKEADKTPKQMDLFEENFLKREVKAEYTLIGQ
                     VFDTYWLVQFQDSLYIIDQHAAHERVMYERTLKEMKSREYTSQYLSPPIILSLSMQEA
                     QLLEEHIDRFTRIGFEIEHFGGEEYAVRAVPDNLFGIAKKELLMEMIDDLADGINTSM
                     TPDLIDEKVASMSCKAAVKGNNRLSAKEVDALIGE"
     assembly_gap    4736..4745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4790..5737
                     /gene="miaA"
                     /locus_tag="ACZPD4_03825"
     CDS             4790..5737
                     /gene="miaA"
                     /locus_tag="ACZPD4_03825"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1378235.1"
                     /translation="MKKPLIILAGPTAVGKTKASIGLAKRIGAEIISADSMQVYKHMD
                     IGSAKITRSEMSGISHHLIDILEPEEEFNVVRFQKMALEEMEQIYEKGKIPLIVGGTG
                     FYIQSVLYNIDFTENDSPNDYRKKLEILAKEKGAEYLHTMLTEVDPKSALQIHTNNIK
                     RTIRALEFYHQTGMKISEHNEAEREKESPYQYAYFVLTDDRAKLYERIDRRVDLMMKS
                     GLEEEVRFLKDRGVSSTSTAMQGLGYKELYAYMNGEYSLEEAVRIIKRDTRHFAKRQL
                     TWFKREKDVTWIDRAVIGDDEEHILDFMIEELRKKQILV"
     gene            5826..7133
                     /locus_tag="ACZPD4_03830"
     CDS             5826..7133
                     /locus_tag="ACZPD4_03830"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1378236.1"
                     /translation="MSNTETKTIYRQLGISEDVWIYGQKIEEGLKERFAEFDSNAEYN
                     QLKVISAMQQCRVNESCFNYASGYGYNDQGRDTLEEVYAAAFHTEAALVRPQITCGTH
                     ALALALSANLRPGDELLSPVGKPYDTLEEVIGIRQSKGSLAEYGVTYKQVDLLEDGYF
                     DYPAIEKALNERTKLVTIQRSKGYQTRPSYSVEKIGELIAFIKKKRPDVICMVDNCYG
                     EFVERIEPSDVGADMIVGSLIKNPGGGLAPIGGYVAGKKELIENCAYRLTSPGLGKEV
                     GASLGVMQSFYQGFFMAPTVVCGALKGAVFAANIYEKLGYSVVPNASESRHDIIQAVT
                     FGSADGVIAFCQGIQAAAPVDSYVTPEPWAMPGYDSEVIMAAGAFVQGSSIELSADGP
                     IKPPYAVYFQGGLTWQHAKLGILKSLQSLIDAGVIDRSKLQSL"
     gene            7174..8403
                     /locus_tag="ACZPD4_03835"
     CDS             7174..8403
                     /locus_tag="ACZPD4_03835"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1378237.1"
                     /translation="MRKVAIIGAGAAGMTAAITAASNGAEVVVLEHMDRVGKKILSTG
                     NGRCNFTNTYQDRSCYHSDNESFPWKIIEKFNAEQIIKLFEELGVYAKNRNGYMYPYS
                     DQASSVTEALKMELERLQIDVRLQTECTDIFPRKKGFTLQIVKDGKKGKIYADHVILC
                     TGSRAFPASGSDGSGYDLAKKLGHKIIPVLPALVQLRCEEKFFKSIAGVRVQGTVSIW
                     SENQCLAKDTGELQLTNYGISGIPVFQVSRYAAIALYKKKKVQAILDFMPEFSEDELE
                     KFLIKRAKMRPYKRADQFFTGLSNKKLSDLWVKEAGISKEKKVGVFAEKEIQQLVRLI
                     KNFKVIVTETNSYEQAQVCRGGIDTKDVHPDTLESRYVPGLYFAGEILDVDGMCGGYN
                     LTWAWASGAVSGREASF"
     gene            complement(8505..9122)
                     /gene="lexA"
                     /locus_tag="ACZPD4_03840"
     CDS             complement(8505..9122)
                     /gene="lexA"
                     /locus_tag="ACZPD4_03840"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR1378238.1"
                     /translation="MSQGKISKKQQEILEYIKSQILQRGFPPAVREICEAVNLKSTSS
                     VHSHLETLEKNGYIRRDPTKPRAIEILDDMFNLTRREMVHVPIIGQVAAGEPILAQEN
                     IEDYWPVPADRMPNKQTFFLKVKGESMINAGILDGDMVLVEEDSTASNGDMVVALIED
                     GATVKTFYKEEGVFRLQPENDFMDPIIVKNVTILGKVIAVMRFFS"
BASE COUNT         3165 a         1588 c         2212 g         2495 t
ORIGIN      
        1 tactaccata gaataagagc gtagtggtac tatgtttaaa actgttattt ttatgtacaa
       61 aggagtagga atgtggctga attaacacca atgatgaagc agtatatgga gacaaagtct
      121 caatatcaag actgcattct attttataga ttgggtgatt tctatgaaat gttttttgaa
      181 gatgccctga ctgcatcaag ggagttggaa atcactttaa ccgggaaaaa ctgcgggcag
      241 gaagaaaagg cgcctatgtg cggagttccg tatcatgccg tagaaggata tttaaacaga
      301 cttgttgcca aaggctataa agtggcaatc tgcgaacagg tagaagatcc gaaaacgacg
      361 aaaggaatcg ttaaaaggga agttgtacgt atcgtaaccc cgggaacaaa tcttgataca
      421 caagcgttgg acgaaacaaa gaacaactat attatgtgta ttgtatatat tgcagacagg
      481 tacggcgttt ctgttgccga tatttcgacg ggagattatt ttgtaacgga gattccggac
      541 agtgcgaaat tattagatga aatctatcga ttctcaccgt cggagattat atgcaatgaa
      601 gcgttttata tgagcggcgt cgacatggac ggaatgaaag atcggcttgg aattacgatt
      661 tattcgcttg aatcatggta ttttgacgat gaagtatgcc gtaaaaaact tttggaacat
      721 tttgaggttt cctcgtttgc cggacttgga cttgcagatt atgattgcgg aatcatcagc
      781 gccggagctc ttctgcagta tctgttggaa acacagaaaa actcgctttc aaatctgaca
      841 catattacgc cttatgccgc aggcaagttt atgatgatcg acagttcgac aagaagaaat
      901 cttgaacttt gcgaaactct ccgtgaaaaa caaaaaaggg gatctcttct gtgggttctc
      961 gataaaacaa agacagcaat gggagctcgt acattgagaa aatatgtgga gcagccgctg
     1021 atcgataaga cagagattat tcgtcgtctg gatgcagtac aggaattgaa agaacaggcg
     1081 atttctcgtg aagaaatcag agaatatctg tctcctgtgt atgatttgga acgactgatc
     1141 acaaagatag cgtacggatc tgccaatcca agagacctga ctgcctttag aagttctctg
     1201 gagatgcttc ccgcactttt gtatatattg caagaaatga aagcagaact tttaaaagat
     1261 cttgctgttg atcttgatcc tcttgaagat ctctgtatac ttgtaaaaaa agcaatccgg
     1321 gaagatcctc ccattgcgat gaaagaagga aatattatta atgacggtta taacgaagaa
     1381 gttgataagc tgcgccgcgc aaagtccgat ggaaaagact ggttggcaaa gcttgaaaat
     1441 gacgagcgtg aaaagaccgg gattaaaaat ctaaagatta agtacaataa agtattcgga
     1501 tattatttag aggtgacaaa ctcttacaaa gaaatggttc cggaatatta tacacggaaa
     1561 cagacattgg caaatgcaga aagatatatt actcccgagc tgaaagaatt agaagacatg
     1621 atacttggag cggaggacaa actttatgca ttggaatatg aactgtacag tgaagtaaga
     1681 gatttgattg caagtcagat agagcgtatc caaaaaaccg ctaaagcagt agcggcgctt
     1741 gacgcctttg cttcccttgc tctcgttgcg gaacgcaata actatgttcg gcctaagatc
     1801 aacgaaaaag gagtaattga tataaaagaa ggacgtcacc ctgttgtaga gcggatgatc
     1861 ccgaatgaaa tgtttatttc aaacgataca tatcttgatg ataaaaaaca ccggatttct
     1921 attattacag gaccgaatat ggcaggaaaa tcgacgtata tgcgtcaaac ggcgctcatt
     1981 gctcttatgg cgcagatcgg ctcgtttgta cctgccaaaa gcgctaatat cggtctttca
     2041 gaccgtattt tcacaagagt cggagcctcg gacgatcttg cttccggtca gagtacattt
     2101 atggttgaaa tgacggaagt tgcaaatatt cttagaaacg ctacgtcaaa aagcctgttg
     2161 attcttgatg aaatcgggag aggcacaagt acgtttgacg gattgtcgat tgcctgggcg
     2221 gtgatcgaat atataagcga cagcagactt ttaggtgcaa aaacgctgtt cgcaacacat
     2281 tatcatgaac tgacagaact ggaaggaaaa atcgataatg taaacaatta ctgtatcgcg
     2341 gtaaaagaaa aaggtgatga cattgtattt ttacgaaaga ttgtcaaagg cggagcagat
     2401 aaaagttacg gaattcaagt tgcaaaactt gcaggtgttc cggagcttgt gatcggccgc
     2461 gcaaaagaga ttgtagaaga gctaagtgat gaagatatta cggcaagagt aagtgagatt
     2521 gcgtcaaaag aacgtgtcgt aaaaaagaaa ccgaaagtaa aaaaatacga tgatgtagat
     2581 attgcgcaga tgtcgctctt tgacacagta aaagatgacg atgttcttga agaattgaaa
     2641 aatcttgacg ttggaaatat gactccgatc gatgctctta atacgatata cagattacag
     2701 aataaattaa agaaccgttg gtaaagttga taaaatcaaa aagaaaagga gcagtcatgc
     2761 ctcatattca agttttagat cagataacga ttgataagat cgcagccggg gaagttattg
     2821 aaagacccgc gtctattgtg aaagaactgg ttgaaaatgc gattgatgcg aaagccacct
     2881 ctgttacagt cgaaattaaa gacggaggaa tttcatttat ccgtgttaca gataatggat
     2941 gcgggattga agcagatgaa gtaagatgtg cttttttgcg ccattcaact agtaagatcc
     3001 gtacagcgga agaccttgta tccatacatt ctctcggatt tcgtggggag gctttgtcga
     3061 gtattgccgc ggtaacaaga acagaagtta tcacaaaaac agaggaagga caaagtggaa
     3121 taaaatatgt catagaaggc ggaaaagaga ctgcattgga agagacagga gctcccaacg
     3181 gaacgacttt tcttattcat cagttatttt ataatatacc cgcgaggcga aaatttttaa
     3241 aaacaccggt gacagaggcg gggcatgtgc aggatctgtt gatccatctg gcgctgtcac
     3301 atccggaagt ttcattttta tttatcaata acggacaaga aaaacttcgg acgtcaggaa
     3361 acggaaaact aaaagatgtg atttataatg tatatggacg cgaagtgacg tcgaatcttt
     3421 tggaacttga ttataaaaaa gacggtcttt ctatttccgg atttttaggt aaaccggtca
     3481 tcacccgagg aaaccggaat tttgaaaatt tctttgtgaa cgggcgttat gtaaaaagtg
     3541 caatgctctc taagtcgctg gaagacgctt ataaagattt tacgatgcag cataaatttc
     3601 cgtttgctgt tcttcatttc caggtagaag gggatcttat tgacgtaaat gttcacccga
     3661 cgaaaatgga gcttcgtttt caacgtcagc aggaagttta caatacaatg tacgaagctg
     3721 ttcacagaag gcttcttgaa cctgaattga ttcaaacagc tacagttccg gatccggcag
     3781 atgcagctca aaagaaaaaa gaaagtccgt ttttgctgaa acccaaatct atagaaaata
     3841 caaaatatga tccaatagaa aaaatccggg aaaagatgca ggaaagagaa ccggagcaag
     3901 tacgggagaa agcgcaggaa acgaaaccgg agcaagtacg ggaaaaagta caggatgaag
     3961 aacaggggca agcccggaaa cagacgtctg ctatcgaggc agataaagaa tctgttttgc
     4021 agtctgaacc tcaaattcat gatgaagatt attttataag gaaaatgcgg gaaagggtga
     4081 aggcatatca tgaacgctcc tcgtctgcag aagttttaga ccgtggggaa atttatcgtc
     4141 cgcagcaaca ggcagaacga atccgggaag ctgctgcata tcgggcaaaa gaggcagaca
     4201 agactccgaa gcagatggat ctgtttgaag aaaatttctt gaagcgtgaa gtgaaggcag
     4261 aatacacatt gatcggtcag gtgttcgata catactggct tgtacaattc caagacagtc
     4321 tttatattat agatcagcat gccgcgcatg aaagagtgat gtatgagaga actttaaaag
     4381 agatgaaatc cagggaatat acatctcagt atttaagccc tccgatcatt ttatcgcttt
     4441 cgatgcagga agctcagctt ttggaggagc atatagatcg ttttacgaga atcggttttg
     4501 aaatcgaaca ttttggagga gaagaatatg ctgtcagggc agtgccggat aatttgttcg
     4561 gtatcgcaaa aaaagaactt cttatggaga tgatcgacga tctggcagat gggataaaca
     4621 catctatgac tcctgatttg attgatgaaa aggtagcgtc tatgtcttgt aaggcggcgg
     4681 taaaaggcaa taaccgttta tcggcaaaag aagtagacgc tctgatcggt gaattnnnnn
     4741 nnnnntgacg aaacgagaat tagagaagaa gtttaagagg atggtataaa tgaaaaaacc
     4801 actgattatt ttggccggac ctacagcggt cggaaaaaca aaagcatcta tagggcttgc
     4861 aaaaagaata ggggcagaaa ttatttcggc agattctatg caggtatata aacatatgga
     4921 tatcggttcg gcaaagatta cgcgcagtga aatgagcggg atttcgcatc acttgattga
     4981 tattctcgag ccggaagagg aatttaatgt tgttcgtttt cagaagatgg ctcttgagga
     5041 aatggaacag atttatgaaa agggaaaaat tcctttgatt gtaggaggaa ccggttttta
     5101 tatccaatcg gttctttata atattgattt tacggaaaat gattctccaa atgattatcg
     5161 gaaaaaactg gagattctcg caaaggaaaa aggggcagaa tacttgcata caatgttgac
     5221 ggaagtagat cctaagtcgg cattgcagat tcatacaaat aatataaaac ggacaatacg
     5281 tgcgcttgaa ttttatcatc agacaggaat gaaaatatcc gaacataatg aagcggaaag
     5341 agaaaaggaa tctccgtatc aatatgcata ttttgttttg acagacgaca gagcaaaact
     5401 gtatgaacgt attgaccgac gcgtagatct tatgatgaaa tcaggtttag aagaggaagt
     5461 tcgtttctta aaagaccgag gcgtgagttc aacttctaca gcgatgcagg gacttgggta
     5521 taaagaattg tatgcatata tgaacggaga gtattcattg gaagaggcgg tacgtattat
     5581 aaaacgcgat acaagacatt ttgccaagcg ccagcttaca tggtttaagc gagaaaagga
     5641 tgtcacatgg atagatcgtg ccgttatcgg agacgacgag gaacatattc ttgattttat
     5701 gatcgaagag ttgagaaaaa agcagatttt agtataaaat ataaaaccaa caaaataatg
     5761 gatcaatata acgaaaactt tacaagatta aagagaaaaa ctaataaaaa aagaggaaaa
     5821 tatatatgag taatacagaa acaaaaacga tataccgaca gttgggaatt tcagaagatg
     5881 tgtggatata tgggcagaag atagaggaag gtctgaaaga acgatttgca gaattcgaca
     5941 gtaatgcaga atataatcaa ctgaaggtga tctctgccat gcagcaatgc cgggtaaatg
     6001 aaagctgctt taattatgca agtggatacg gttataacga tcaaggacgc gatacgctgg
     6061 aagaagtata tgcggcggca tttcatacag aagcggctct tgtgcgtccg cagattacat
     6121 gcggtacaca tgcacttgca cttgcgttat cggcaaactt aagacccggt gatgaattgt
     6181 tatctcctgt agggaaaccc tacgatacac tggaagaagt aatcggcatt cgccagtcta
     6241 aaggatctct cgcagaatat ggagtgacat ataaacaggt cgatctgttg gaagacggtt
     6301 attttgatta tccggcaatc gaaaaagcat taaacgaaag aacaaagctt gtgacgatcc
     6361 agcgttcaaa aggatatcag acacgtccga gttattctgt agaaaaaata ggagaactga
     6421 ttgcatttat aaaaaagaaa agaccggatg ttatttgtat ggtagataac tgctacggag
     6481 aatttgtaga acggatcgaa ccaagcgatg taggtgcaga tatgattgtc ggttcattga
     6541 ttaaaaatcc cggcggcggt cttgcaccga tcggaggata tgtggcaggc aaaaaagaac
     6601 ttatcgaaaa ctgtgcgtat agattgacat cccccggatt agggaaagaa gtgggagcgt
     6661 ctctcggagt gatgcagtct ttttatcaag gattttttat ggcgccgaca gttgtatgcg
     6721 gagcgcttaa aggagctgtt tttgcagcga atatttatga aaaactcggg tattcggttg
     6781 ttccaaatgc aagcgagagc cgtcatgata ttattcaggc agttacgttt ggcagtgcag
     6841 atggagtgat tgcattttgc cagggaattc aggcagcggc tccggtagac agttatgtaa
     6901 ctcccgaacc ttgggcaatg ccgggatacg acagtgaagt catcatggct gccggagcat
     6961 ttgtacaagg gtcctcgatt gaactatctg cagatggtcc gatcaaacct ccctatgccg
     7021 tttattttca aggcggtctt acatggcagc atgcgaagct gggcatttta aaatctcttc
     7081 aaagtttgat tgatgcagga gttattgaca gatccaaact tcagtcgctt taaaacagtg
     7141 ttttgatgct tagaaaagaa aaggatttat attatgagaa aagtagcgat aatcggggcg
     7201 ggagcagccg ggatgacggc agcgatcaca gcagcttcaa acggggcaga agtagttgta
     7261 ttggaacata tggaccgcgt cgggaaaaaa attctttcga cagggaacgg acgatgcaat
     7321 tttacaaata cttatcagga tcgaagctgt tatcactctg ataacgaatc atttccatgg
     7381 aagatcattg agaaatttaa tgcagagcag attataaaat tgttcgaaga actcggcgtt
     7441 tatgcgaaaa acagaaatgg atacatgtat ccctattcgg atcaggcatc gtctgtaact
     7501 gaggctttga aaatggaatt agagcgtctg caaatcgatg taagacttca aacagaatgc
     7561 acggatattt tccctcgtaa aaaggggttt actcttcaga ttgttaaaga cgggaaaaag
     7621 gggaaaatat atgcggatca tgtaatcctg tgtacgggat ccagagcttt ccctgccagc
     7681 ggatccgatg gttccggtta tgatctggca aaaaaactcg gacataaaat aattcctgtt
     7741 cttcctgctc ttgtacaatt aagatgtgaa gaaaaatttt ttaaatcaat cgcaggagta
     7801 agagttcagg gaactgtttc gatatggtcg gaaaatcaat gtctggcaaa agatacggga
     7861 gaattacaac tgaccaatta tggcatatca ggtattcctg tatttcaagt gagcagatat
     7921 gcggcgattg cattatataa aaagaaaaaa gttcaggcga ttcttgattt tatgccggaa
     7981 ttttcggaag atgagctgga aaagttttta ataaaacggg cgaaaatgcg tccgtataag
     8041 agagcagatc aatttttcac agggttatct aataaaaagt tgtccgatct ttgggtgaaa
     8101 gaagcaggga tttcaaaaga gaaaaaagtc ggagtgtttg cagaaaaaga aattcagcag
     8161 cttgtccgtt taatcaagaa ttttaaagtg attgtaacag aaacaaattc ttatgagcag
     8221 gcacaggttt gccgaggagg tatcgataca aaggacgttc atcctgatac tttagaatcg
     8281 cgttatgttc cgggacttta ttttgcagga gaaatactag acgtagatgg catgtgcggt
     8341 ggatataatc tgacatgggc atgggcgagc ggtgcagtca gcggaagaga agcaagtttt
     8401 tagaaaacct gattaaaaac caaaaagaga agtatccaaa atctttaaat acaggatact
     8461 tctctttttg tcttatgatt ttttcggtac tttatattcg gaagttatga gaaaaatctc
     8521 atcacagcaa taactttacc taaaatagta acattcttta caatgatcgg atccataaaa
     8581 tcattttcag gctggagacg aaaaactcct tcttccttat aaaatgtttt tacggttgct
     8641 ccgtcctcga tcaacgcaac gaccatatct ccattagaag ctgtggaatc ttcttccaca
     8701 agaaccatat cgccgtcaag tataccggca ttgatcatac tttctccttt tacctttaaa
     8761 aaaaatgtct gtttattcgg catacgatca gcgggaaccg gccagtaatc ttcaatgttt
     8821 tcctgagcca gaatcggttc tcctgcggca acttgcccga taatcggtac atgaaccatt
     8881 tcacgccgcg tcaggttgaa catatcgtcc aaaatttcga tcgcacgggg ttttgtcgga
     8941 tcgcgccgaa tataaccgtt tttttctaat gtttcaagat gtgaatgaac agaagaagta
     9001 gactttaaat ttacagcttc gcatatttcc cgaacagcag gcggaaaccc ccgttgaaga
     9061 atctgtgatt taatatattc taaaatttcc tgttgttttt tacttatttt tccttgtgac
     9121 atagtttcct ccgatattat agtccgttta ttttattaaa ataatggtaa cacaacgaag
     9181 aacaaaaagc aaacatttgt tgcgaaaata tgttcgattt ccgtgttgac aaacatttgt
     9241 tcttattgta taatccaaat atagaacata agttcgcgga acaaatattc gaatcgaaga
     9301 atacatgtat acatttttct tgcagactga atattacaaa ataataagag aaaacaaagt
     9361 gtaaaaataa gcagagataa aggggatact attatgaagg gaaaaagaaa tgaaaaaaga
     9421 tcaaaaaaat cggctatatt aaaacaacat gctcgtaatt tattcgtggg
//
LOCUS       DBMDXF010000061         9462 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_1980_length_9462_cov_2.393655, whole genome shotgun sequence.
ACCESSION   DBMDXF010000061 DBMDXF010000000
VERSION     DBMDXF010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_9462_cov_2.393655"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            179..2431
                     /gene="pflB"
                     /locus_tag="ACZPD4_03845"
     CDS             179..2431
                     /gene="pflB"
                     /locus_tag="ACZPD4_03845"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR1378239.1"
                     /translation="MAQFDQWAGFSGKNWKNSVDVRDFIQENYTPYDGDESFLEGPTE
                     ATDKLWGRLQELQKEERAKGGVLDMETEVVSGLTAYGPGYIDESMKDLEKVVGLQTDK
                     PLKRAFMPYGGINMAVKACTTYGYEPNERLKEIFTKYHKTHNQGVFDIYTPEMKKARH
                     NKIITGLPDTYGRGRIVGDYRRVALYGIDFLIEKKQYDFERYARHGMKGTDFRLREEI
                     ADQIRALQQMKEMAAIYGYDISQPAKDAKEAVQWLYFGYLAAIKTQNGAAMSVGRIST
                     FLDIYIERDMKNGVLTESEAQELIDHMVMKFRMVKFARIPSYNELFSGDPVWATLEMG
                     GLGQDGRSMVTKNDFRFLHTLENMGPSPEPNLTVLYSERLPENFKKYTSLISVKTSSV
                     QYENDDVMRPVWGDDYSICCCVSATQTGKEIQFFGARANLAKCLLYAINGGVDEKTKV
                     QVAPAYRPITSEYLDYDEVMEKYDEMMEWLSKLYVDTLNMIHYMHDKYYYEAAEMALI
                     DTDVRRTFATGIAGFSHVVDSLSAIKYAKVKVIRDEDGLATDFEIEGDFPRYGNDDDR
                     ADDIAIWLLKTFMHKLSKCHTYRDSEPTTSILTITSNVVYGKATGTLPDGRKAGEPLS
                     PGANPSYGAEKNGLLASLNSVAKLPYELALDGISNTQTISPNALGHDDDERKVNLARV
                     MDGYFHQGAHHLNVNVFGTDKLIDAMEHPEKPEYANFTIRVSGYAVKFIDLTREQQLD
                     VIARTCHESM"
     gene            2638..3390
                     /gene="pflA"
                     /locus_tag="ACZPD4_03850"
     CDS             2638..3390
                     /gene="pflA"
                     /locus_tag="ACZPD4_03850"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR1378240.1"
                     /translation="MEQANEIKGYVHSLESFGSVDGPGVRYVIFLTGCAMRCQFCHNP
                     DTWNLKKGTLYTADELLKTALRYRTYWGDKGGITVSGGEPLLQIDFLIELFRKAKEAG
                     VHTTLDTSGNPFTREEPFFGKVQELMKYTDLVMLDIKHIDDEQHKILTGCTNENILDF
                     AKYLDEIKKPVWIRHVLVPQRSDDDGALGKLREFIDGLSNVEKVEVLPYHTLGAYKWK
                     ELGYEYPLEGIDPPTKERIENANKILRTAECK"
     gene            complement(3466..4806)
                     /locus_tag="ACZPD4_03855"
     CDS             complement(3466..4806)
                     /locus_tag="ACZPD4_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378241.1"
                     /translation="MNAARQNEQSIFHTFFKYVSSNVLGMIGFSCYILADVFFIARGI
                     GADALAALNLTLPAYNLMNGIGLMIGMGGAARYSLSSTRPDSDTHRTAFTHALLLAAL
                     CALFFSFAGAFCSEEISAILGADHDTIGYASDYIRILLLFSPLFLGNNLLLCFVRNDG
                     APRLSMAGMLIGSLANIVLDYIFIYPLGMEMAGAATATATAPVISMLIMSVHFIKKNN
                     RFHITKIRLSLKRAADICFLGVSSLVLELSSGIVIIVFNFLILKLNGNTGVAAYGILA
                     NIALVLTSVFTGIAQGIQPIVSRHADAKGKHVQNSIRKYALITSLLLSLVSYITISLF
                     SVPIAEAFNKADDPLLTTIASGGMRIYFINLFFAGVNIIASAFLCSCDKPKLAFVISV
                     LRGFILIIPAAWILSSLFGLTGIWLTVPVTEAIVCLFSFLFLFRGTHTQIVQST"
     gene            5138..6301
                     /locus_tag="ACZPD4_03860"
     CDS             5138..6301
                     /locus_tag="ACZPD4_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 domain-containing protein"
                     /protein_id="HIR1378242.1"
                     /translation="MNKKEILEIRKQFTPANCAITRICGCYVDHEKEKKMESKEAFLS
                     LPEEEAFKYFDIFKKTLSGTVGKNILNMEFPIDAEMPGGTQEFLLKLRSSKLQDDMLL
                     EEFYDKVIEAYDCAENYYIILIHAMYDVPGKSSDGIEMFDASDEVYEYLLMSLCPVSL
                     SKAGLSYNAESNCIQDRIRDWIVDMPDKGFLFPAFNDRSTDLHSMLYYTKKSEDLQPG
                     MIDQLFGTKMPMSADTQKETFKMLIEDTLGEDGDYETVRNIHETLNQLIEEHKEEPEP
                     LALDLTDMKKVFEQSGVEAEKMESFDRNFTENAGEKTSLLASNIAETRKFQIETPDIV
                     IKVNPERADLVETRIIDGRRCLVIPVDDHIEVNGINVQTIKMKEREENQETYN"
     gene            6438..6893
                     /locus_tag="ACZPD4_03865"
     CDS             6438..6893
                     /locus_tag="ACZPD4_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1378243.1"
                     /translation="MFQCDINDAPVIGILGIVSHRAKVAAKDMYSVFDLNRSQASILL
                     TLHHCESMSQKDLAKQLNITAPSITSSIRKMEQFGYIARQTDPADQRVMRLALTEKGR
                     SCIQSIKDVTDKMEEILLKGMSVEEKILFRRLLVHVNNNIDQYERKEKI"
     gene            6890..9214
                     /locus_tag="ACZPD4_03870"
     CDS             6890..9214
                     /locus_tag="ACZPD4_03870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378244.1"
                     /translation="MKNLFKYASEHWKALLAIVAILIVQAYCDLSLPAYTSDIVNVGI
                     QQGGVEDHIPDAISAEDMETLLLFTSEKDGKTVLSAYEKDDKTYEEQAYVLKDTVKED
                     TDRTEKLSGILAAPMMMAAGFESGSDMTADIEEQLKAQLPPEMISEDMTVLDILKMMP
                     QEQKQAFVSEIEKKTEELPDTITEQAAVNYVKEAYADLGIDMDELQFRYLFSTGAKMI
                     GLAFLGMAASVLVGFLASRVGAAAGRDLRGRVFKKVVGYSSNEFDHFSTASLITRSTN
                     DIQQVQFIIVMLLRIVLYAPILAIGGIVQVFQTNVSMSWIIGLAVVLIALVVLVLFAV
                     AMPKFKSLQKLVDKVNLVMREILTGLPVIRAFSTEKHEEKRFDKANMDLMKTNLFVNR
                     AMTFMMPIMMLIMNGISVLIMWSGAKGINDGQMQVGDMMAFIQYTMQIIMGFLMICML
                     SVMLPRAAVAADRVDEVLSSRTVIHDPKTPKKFEEKQKGVLTFDHVSFKYPGADENVL
                     EDITFTAKPGETTAIIGSTGSGKSTLVNLIPRFYDVTDGSIRLDGTDIREVTQHDLRD
                     KLGYVPQKGLLFSGDIASNIMFGNPEGGGQEMKEAAQIAQATEFIEAKPNKYESPISQ
                     GGSNVSGGQKQRLSIARAIAKRPEVFIFDDSFSALDFKTDVALRKALKKRTKESTVLI
                     VAQRISTILNAEQIIVLDDGKIAGIGTHKELLRNCEVYKQIAASQLSEKELQDGMQSE
                     DRKTEDIKSETIKTEERKEAISAKTAEQEVRSDV"
     gene            9207..>9462
                     /locus_tag="ACZPD4_03875"
     CDS             9207..>9462
                     /locus_tag="ACZPD4_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378245.1"
                     /translation="MSRHGMKGPHGGMRGGAKAKDFKGSLKKLVKYMSVYKVQMLFVA
                     VFAVCGTVFNIVGPKILGKATTEIFNGLVSKVSGGSGMDFG"
BASE COUNT         3053 a         1706 c         2377 g         2326 t
ORIGIN      
        1 gcgttatttt tgatatggac gggctgatgt ttgacaccga gcgcctgtgg caatgagata
       61 caaattacga tatcatttgg actgtttaac cgctcattgc atgaaccgaa agcgcagatg
      121 atgtgcttcg gtatgaaacg aataaattta ttaagatttc agaggaggct ttcaaattat
      181 ggcacaattt gatcagtggg caggattttc cggaaagaac tggaaaaatt ctgtagatgt
      241 acgcgatttc attcaggaga actacacacc gtatgacgga gacgaatcat tcctggaagg
      301 acctacagag gcaacagata aactgtgggg aagacttcag gagctgcaga aagaggaacg
      361 tgcaaaaggc ggcgttcttg acatggagac agaagttgta tccggtctta cagcatacgg
      421 cccgggatat atcgacgaga gcatgaaaga cctggagaaa gttgtcggac ttcagacaga
      481 caaaccgctt aagagagcgt ttatgcctta cggtggtatc aacatggcag taaaggcttg
      541 tacaacttac ggttatgagc cgaatgaaag attgaaagag atctttacaa aatatcataa
      601 aacacataac cagggagtat tcgacatcta cacaccggag atgaagaaag cccgtcacaa
      661 caagatcatc acaggacttc cggatacata cggacgcgga cgtatcgtag gcgactaccg
      721 tcgtgttgct ctgtacggta ttgatttcct gatcgagaaa aaacagtacg acttcgagcg
      781 ttatgcaaga cacggcatga aaggaacaga cttccgtctt cgtgaagaga tcgcagatca
      841 gattcgcgca cttcagcaga tgaaagagat ggcagcaatc tacggatatg atatttcaca
      901 gccggcaaaa gatgcaaaag aagcagtaca gtggctgtat ttcggatacc ttgcggcgat
      961 caagacacag aacggagctg caatgagtgt cggacgtatc tctacattcc ttgatattta
     1021 tattgaaaga gatatgaaga acggtgttct gacagagagc gaagctcagg aattgatcga
     1081 ccacatggtt atgaagttcc gtatggttaa attcgcaaga atcccgtcat acaacgagct
     1141 gttctcagga gacccggtat gggcaacact tgaaatgggt ggtctcggac aggacggacg
     1201 ttctatggta acaaagaacg acttccgttt ccttcacaca cttgagaaca tgggaccgtc
     1261 tccggagccg aacctgacag ttctttactc agaaagactt ccggaaaact tcaagaaata
     1321 tacatctttg atttctgtaa agacaagttc cgtacagtac gaaaacgatg atgttatgcg
     1381 tccggtttgg ggcgatgact acagcatctg ctgctgtgta tccgcaactc agacaggtaa
     1441 agagatccag ttcttcggag caagagcaaa ccttgcaaaa tgcctgcttt acgcgatcaa
     1501 cggcggtgtt gacgagaaaa caaaagtaca agtagctccg gcttacagac caatcacatc
     1561 agagtatctt gattacgatg aagtaatgga gaaatatgac gagatgatgg agtggctgtc
     1621 caaactgtat gttgatacat tgaacatgat ccactacatg catgacaaat actactatga
     1681 agcagcagag atggctctta tcgatacaga tgtaagacgt acatttgcaa caggtatcgc
     1741 aggattctca catgtagtag actccttaag cgcaatcaaa tatgcgaaag taaaagtaat
     1801 tcgtgacgaa gacggtcttg caacagactt tgagatcgag ggagacttcc cgagatacgg
     1861 taacgatgac gaccgtgcag acgatattgc gatctggctg ctcaagacat ttatgcacaa
     1921 attgagcaaa tgccacacat acagagattc tgagcctaca acatcgatcc tgacaattac
     1981 atcaaacgta gtttacggaa aagcaacagg aactcttccg gatggaagaa aagcaggcga
     2041 gccgctgtca ccgggagcaa acccgtctta cggcgcagag aagaacggtc ttcttgcttc
     2101 cctgaactct gtagcaaaac ttccgtacga gctggctctt gacggaatct ctaatacaca
     2161 gacgatcagc ccgaatgcac tcggacatga tgatgacgag cgtaaagtaa atcttgcccg
     2221 cgtaatggac ggatacttcc atcagggagc acaccacctg aacgtaaacg tattcggtac
     2281 agataaactg atcgacgcta tggagcaccc ggaaaaaccg gaatatgcaa actttacaat
     2341 ccgtgtatca ggatatgccg ttaagtttat cgacctgaca agagagcagc agttagacgt
     2401 aatcgcaaga acttgccacg agtctatgta ataaatagta acagttcagt ccgcgccatt
     2461 cgtaaaactg cactgtatgg ctgaactgtt acaataaata aaggtctgca gatttcttta
     2521 gaaagaaaaa tgtgactata agatgaagaa agtccggttc ttttgcttga gatatgatct
     2581 ttggcagaag aaccggtttc tttataaatc tttataaaat gaaaaggaga cgccggaatg
     2641 gaacaggcaa acgaaataaa aggatacgtt cattcacttg aaagttttgg atcagtagac
     2701 ggaccgggag tacgatacgt gatctttttg acaggatgcg ctatgcgctg tcagttctgt
     2761 cataacccgg atacatggaa tcttaagaaa ggaacgctgt atacggcaga tgagcttctg
     2821 aaaacagctc tgcgctatcg cacttactgg ggagataaag gcgggatcac ggtaagcgga
     2881 ggagagcctc ttcttcagat tgacttcttg attgaattgt tccgcaaagc gaaggaagcc
     2941 ggagtccata caacgcttga tacaagtgga aatccgttta cgagagaaga accgtttttc
     3001 gggaaagtac aggaactgat gaaatataca gatcttgtga tgctcgatat aaaacatatc
     3061 gatgatgagc agcataagat attaacagga tgtacgaatg aaaatattct tgatttcgct
     3121 aaatatttgg atgagattaa aaaacctgtc tggattcgtc atgtccttgt tccgcagagg
     3181 agcgacgatg acggagcgct tggaaaactc cgtgaattca ttgacggact aagtaatgtg
     3241 gaaaaagtcg aagtccttcc gtatcatacg ctcggagcat ataaatggaa agaactcgga
     3301 tatgagtatc cgttggaggg gattgatcct ccgacaaaag agcggattga aaatgcaaat
     3361 aaaatactgc gtacagcgga atgtaagtaa aaacgaaagt atcgggatac agaagatgaa
     3421 ttttgtatcc cgatactttt tgatacgcct gaaagagtgg gcgttttatg ttgactgtac
     3481 tatttgagtg tgtgtgccgc gaaaaagaaa caggaatgaa aacaggcata caatcgcttc
     3541 ggtgacagga acggtaagcc agatccctgt caatccaaat aatgacgaca aaatccatgc
     3601 ggcaggaatg atcagtataa agcctcttaa aacagagatg acaaatgcaa gttttggttt
     3661 gtcgcatgaa cataaaaatg cagatgctat aatgttcact ccggcaaaaa ataagttgat
     3721 aaaataaatt ctcatgcctc cggaagcgat agttgtcaac agaggatcat ccgccttgtt
     3781 gaatgcttcg gcgataggaa cagaaaaaag actgatcgtt atgtaggaaa caagcgaaag
     3841 gagaaggctt gtgatcagcg catattttcg tatactgttt tgtacatgct ttccttttgc
     3901 atcggcatgt ctgctgacga taggctgtat gccctgcgcg atccctgtaa aaacagaagt
     3961 gaggacaagg gcgatatttg caaggattcc gtaggcggca acacctgtat ttccgttcaa
     4021 cttaaggatc aggaaattga atacgataat aacgattcca gaggaaagct ccagtacaag
     4081 agaggaaact cccaggaaac aaatatcagc ggcgcgtttt aatgacagcc gtatttttgt
     4141 aatatgaaac cggttgtttt tctttataaa atggacagac atgatgagca tactgatcac
     4201 aggagcagtt gcagtcgcag tggcggctcc tgccatttcc atgccaagcg gatagataaa
     4261 aatatagtcc agtacaatgt tggcaagact tccgataagc attcccgcca tagaaagtct
     4321 gggagctccg tcgtttctca caaaacaaag aagaagattg tttccgagaa agagcgggga
     4381 gaagagaagc agtatgcgga tgtagtcgga ggcgtaaccg atcgtgtcgt gatcggcgcc
     4441 gagtatggcg gaaatctctt ctgaacaaaa tgctcccgca aaggagaaga aaagtgcgca
     4501 gagagcggcc aaaagcagag catgtgtaaa agcagtgcgg tgtgtatcgc tgtccgggcg
     4561 tgtcgatgaa agcgagtagc gggcggctcc gcccataccg atcatcagac cgatcccgtt
     4621 cataaggttg tatgcaggga gcgtcagatt gagcgccgcg agggcgtctg cgccgattcc
     4681 ccttgcaatg aaaaaaacgt cggccagaat atagcaggaa aatccgatca tgccgaggac
     4741 gtttgatgat acatatttaa aaaaagtatg gaagatcgat tgttcgtttt gtcttgcagc
     4801 gttcataaaa gttccttccg taaaaaaggc atctgacgat gcattttctc tgaaaatatt
     4861 tcaagaaaga gtgcgaagcc aaatgagttg aaatgtaagc tttctatcaa aagtctattt
     4921 tgtatggtct tattgtttgc gacgctatcg taccatagaa caaagggaaa atcaaatctt
     4981 ttttaattcc ccgatcaggt gtatgatata atagatcggc gcaaacggaa atagcgccgg
     5041 aaaatgagca aaacggaagc agatgcgaaa gaaacagatg cgatgaaagc aggcgcgaaa
     5101 gaaacagaca gaagaatgac ggaaaggggt ttataatatg aataagaagg aaatattaga
     5161 gataagaaag cagtttactc cggcaaattg tgcgattaca cgtatatgcg ggtgttatgt
     5221 agatcatgag aaagaaaaga agatggaatc aaaagaagct tttttgtcat tgccggaaga
     5281 agaggcgttt aaatattttg acatttttaa aaagacgctt tccggtacgg tcgggaaaaa
     5341 tatattgaat atggaatttc cgatcgatgc ggagatgccc gggggaacgc aggagtttct
     5401 tttgaagctt cgcagcagca agcttcagga tgatatgctt ttggaagagt tttatgataa
     5461 agtcatcgag gcatacgact gtgccgagaa ttattacatt attttgatcc atgcgatgta
     5521 tgatgttccg ggaaaatctt ccgacgggat cgaaatgttc gacgcgtctg acgaagtgta
     5581 tgaatatttg cttatgagtc tctgcccggt gtctttgtcg aaagcaggat tgagttataa
     5641 tgcggaaagc aactgcattc aggatcggat aagagactgg atcgtagata tgcccgataa
     5701 aggatttttg ttccctgcct ttaatgaccg aagcacggat ttacatagta tgctatatta
     5761 tacgaagaaa tcagaagatt tacagcccgg aatgatcgat cagcttttcg gaacgaagat
     5821 gccgatgtct gccgatactc agaaagaaac gtttaaaatg ttgatcgaag acacgcttgg
     5881 tgaggacgga gattatgaga ccgtcagaaa tattcatgaa acgctgaatc agttgataga
     5941 agaacataag gaagaaccgg aaccgctggc gcttgatctg acagatatga agaaagtgtt
     6001 tgagcaaagc ggtgtagagg cggaaaagat ggaaagtttc gaccgcaatt tcacggaaaa
     6061 tgcaggagaa aaaacatcgc ttttagcatc taatattgca gagacgagaa agtttcagat
     6121 tgaaactccg gacatcgtta ttaaggtaaa tccggaaaga gcggatctcg ttgagacgag
     6181 gatcattgac ggacgccgct gtcttgtgat accggtggac gatcatatag aagtaaacgg
     6241 aatcaatgtg cagacgatca aaatgaaaga acgggaagaa aatcaggaga catataatta
     6301 acctactaaa taaaattagt caaaaagtat gaaaaaaatg tgaagtcaac atgtatatgt
     6361 tgactttttt gtgttatatt actaatatat tatttagtta tctaactaat aaataaaaag
     6421 taagagggag gcataacatg tttcaatgtg atataaacga tgctccggtg atcgggatat
     6481 tgggaattgt gtcgcaccgg gcgaaagttg cggcaaaaga tatgtacagt gtatttgatc
     6541 taaacagaag tcaggcaagt attttactta cgcttcatca ctgtgagtct atgtcgcaga
     6601 aggatctggc aaagcagttg aatattaccg ctccgtcgat tacatcatcg atccgtaaaa
     6661 tggaacagtt tggatatatt gcaagacaga cagatcctgc ggatcagaga gttatgcggt
     6721 tggcgcttac agagaaagga cgttcgtgca ttcaatctat aaaagacgtg acggataaaa
     6781 tggaggagat cctgcttaaa ggaatgagcg tagaagaaaa aatattgttt cgcaggcttt
     6841 tagttcatgt aaacaataat attgatcaat atgaaaggaa agaaaagata tgaaaaactt
     6901 atttaagtat gcgtccgagc actggaaagc gcttcttgcg atcgtggcca ttttgatcgt
     6961 gcaggcgtat tgtgatctgt ctttgcctgc atatacttcg gacatcgtca atgtgggaat
     7021 ccaacaaggc ggagttgaag atcatatacc ggatgcgatt tccgcggaag atatggagac
     7081 acttttgctt tttacttctg aaaaagacgg aaaaactgtt ttgtcggcat atgaaaagga
     7141 tgacaaaaca tatgaagaac aggcttatgt cttaaaagat actgtaaaag aagatacgga
     7201 taggacggaa aaattaagcg gtattctggc agcgcctatg atgatggcgg caggatttga
     7261 atcaggaagc gatatgaccg cagatatcga agaacagttg aaagcacagc ttcctccgga
     7321 aatgatatcg gaagatatga cggttttgga tattttgaaa atgatgccgc aggagcagaa
     7381 acaggcattt gtatcggaga tagaaaaaaa gacggaagaa cttcctgata cgatcacaga
     7441 gcaggctgcc gtaaattatg taaaagaagc ttatgcagat ctcggtatcg atatggatga
     7501 attgcagttt cgctatctgt tttcaacagg agcaaaaatg atcggtcttg catttctcgg
     7561 aatggcggca agcgttcttg tcggatttct tgcatcccgc gtcggagcgg cggcaggacg
     7621 cgatctaaga ggaagagtgt ttaagaaagt cgtaggatat tcaagcaatg agttcgatca
     7681 tttttccacg gcgtctttga tcacgagaag tacaaacgat atccaacaag tacagtttat
     7741 tatcgtgatg ctgcttagaa ttgtacttta tgcgccgatt cttgcgatcg gaggcattgt
     7801 gcaggtattt cagacaaatg tttcaatgtc ctggatcatc ggtctggcag ttgttttgat
     7861 cgctttagtc gttttggttt tgtttgcggt tgcaatgccg aaatttaaga gcctgcagaa
     7921 attagttgat aaagtgaacc ttgttatgag agaaatattg accggacttc ctgtgatcag
     7981 ggcgtttagt acagagaagc atgaggaaaa gcggtttgac aaagcaaata tggatctgat
     8041 gaaaacaaat ctgttcgtaa atcgcgcgat gacgtttatg atgccgatca tgatgcttat
     8101 tatgaacggg atctcggttt tgatcatgtg gtccggtgca aaaggaatca acgacggaca
     8161 gatgcaggtc ggagatatga tggcgtttat ccagtatacg atgcagatta ttatgggatt
     8221 ccttatgata tgcatgttgt cggttatgct tccaagagcg gctgttgcgg cagatcgtgt
     8281 ggatgaggtt ctgtccagca ggacggtgat ccatgatccg aaaacaccga aaaaatttga
     8341 agaaaaacaa aaaggtgtgc tcacattcga ccatgtttct ttcaaatatc cgggtgcgga
     8401 tgaaaatgtt cttgaagata ttacatttac ggcaaaaccg ggagaaacga cagcgattat
     8461 cggaagtacc ggaagcggaa aatcaacgct tgtcaatctg atcccccgtt tttatgatgt
     8521 gacggacgga agtatccgtt tggatggaac agatattcgt gaagtgacac agcatgattt
     8581 gagagataaa cttgggtatg ttcctcagaa aggactgttg ttctcaggtg atatcgcatc
     8641 gaatattatg ttcgggaatc cggaaggcgg cgggcaggag atgaaagaag ctgcgcagat
     8701 agcgcaggca acagagttta tcgaagcaaa gccgaacaaa tatgaaagtc ctatttcgca
     8761 gggcggatcg aatgtgtccg gaggccagaa acagagactt tcaatcgcaa gggcgatcgc
     8821 aaaacgaccg gaagtgttta tttttgacga cagtttttcg gcgcttgact tcaagacaga
     8881 cgttgctcta cggaaagctt tgaaaaagcg gacgaaagaa agtacggtgc tgatcgtggc
     8941 acagcgaatc agtacgattt taaatgcaga acagatcatt gtacttgatg atggaaagat
     9001 tgcaggaatc ggtacacata aagagcttct tagaaactgt gaagtttaca aacagattgc
     9061 ggcatcccag ttgtctgaaa aagaacttca agatggaatg caaagtgaag acagaaaaac
     9121 ggaagatata aaaagtgaaa cgataaaaac agaagaaaga aaagaagcaa tcagtgcaaa
     9181 aacagcggaa caggaggtga gaagcgatgt ctaggcacgg aatgaaaggt cctcatggag
     9241 gaatgcgagg aggagcaaaa gcaaaggatt ttaaaggctc gcttaaaaaa cttgtaaaat
     9301 atatgagcgt atataaagtg cagatgcttt ttgtcgctgt tttcgcagta tgcggaacgg
     9361 tatttaacat tgtcggaccg aaaatattag gtaaggctac aacagagatt tttaacgggc
     9421 ttgtcagtaa agtatcgggc ggatcgggaa tggatttcgg aa
//
LOCUS       DBMDXF010000062         9356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2009_length_9356_cov_2.064830, whole genome shotgun sequence.
ACCESSION   DBMDXF010000062 DBMDXF010000000
VERSION     DBMDXF010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_9356_cov_2.064830"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="ACZPD4_03880"
     CDS             <1..1125
                     /locus_tag="ACZPD4_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1378246.1"
                     /translation="KYLYKYVDAFGNTKYVYAWRLTPTDPTPKEKREKPSLRELEQQI
                     RRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKSTQKQREQLMRLLKEDKLGARSIDT
                     IKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFYIAIQDDCVRKNPFDFKLSEVLEN
                     DTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLILLKTGLRISELCGLTVADIDFKN
                     EVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSRETIQAFQRVMKKRPKAEPFVIDG
                     QSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKHHKDNPLPHITPHTLRHTFCTRLASK
                     NMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEVQRLIA"
     gene            1293..1745
                     /locus_tag="ACZPD4_03885"
     CDS             1293..1745
                     /locus_tag="ACZPD4_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1378247.1"
                     /translation="MLNFVAHKMQTDNNEGKWKTMNMEAWIGPILCSLQTIIPTVIVV
                     CFGANVVELILEVIPEWVTNGLSIAAGMLPVVGIGMLMRYMPVKKFLPFILVGFVLSA
                     YLGVPVLGIAIIGFAAAFWYFTTEINKADAVTMAAAEHADEMGDDFDE"
     gene            1738..2505
                     /locus_tag="ACZPD4_03890"
     CDS             1738..2505
                     /locus_tag="ACZPD4_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1378248.1"
                     /translation="MSNEMNKEMSQGTDVQTEERYQVTKRDLRKTAARYNFMACNIFN
                     YESQMGPAIAWAMAPVLRKIYKKDDEYKMALNNHFNYFNSTTVMSSMILGATLAVEEK
                     DGIAAKDTVQSLKTSLMGPFAGVGDTLVWVLWPTIIGSISGYMAQQGNPLGAIIWLLC
                     NIAFWFVKSKMFQIGYTSGTKLITNLGKRLTVFTEAASIMGLSVVGALIATSVKITTA
                     LNFKVGEVALNLQTDILDKIMPSLLPVLLTLLVYKLL"
     gene            2637..4067
                     /locus_tag="ACZPD4_03895"
     CDS             2637..4067
                     /locus_tag="ACZPD4_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1378249.1"
                     /translation="MANNYHQLSLKDTFSDCKDMFMDDVPSFFQLLEQHFDISLFIPQ
                     TFYNAFYQHLGRKREYPLTGFLSALILQKIFPIPTDSLLILLLNLCKELRDFCGFSKV
                     PDAPLFTRFRLGFSDHIELMFQQMVDYTEPICQQIDSSLAQILTFDTSGIELYVTENN
                     PKTLNALIRKLKVYYKDIPDVDPYKMAYGLMPSQAASCPDAKQQYINGHFCYADKFAI
                     LTNGLGIIRHISFLDDDFKTAHPEMTIEKKSDSPDEDKSIGDATSLKPVLSDFFFLHP
                     DFHPDTFLGDSSFDTIKTYGMLLNDFHFSKALIPYNLRNESNLKKVGYNEYGYPTCPG
                     DPSLAMKYCGITHENGRADRVKWICPKVHMVKGKYVCDCKNPCSTATKGRTTYTYENL
                     NFRMFPGIQRDSEEWNSLYKIRTIVERAIDHLKINMCVAGRKSRNHTTTKADVFLAGI
                     ASQLTVIVAHSMNCPQYIRSLKPLIA"
     gene            4367..4783
                     /locus_tag="ACZPD4_03900"
     CDS             4367..4783
                     /locus_tag="ACZPD4_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1378250.1"
                     /translation="MKRILIIASHEKMADGMKDTLNFISGGIQETIALSAYVDNRPVE
                     EAVDEVMDGFEGEDEVVVLTDLMVGSVNQKFFKYRTRPHTHIVSGMNLPLAFQIAMEP
                     QGKYITEERMKEIIETAKEELKYVNEVSNDEDEEDE"
     gene            4776..6464
                     /locus_tag="ACZPD4_03905"
     CDS             4776..6464
                     /locus_tag="ACZPD4_03905"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1378251.1"
                     /translation="MNNQTAWWKKSVVYQIYPKSFYDSDGDGFGDLNGIREKLSYLEK
                     LGVDVIWLNPIYESPQVDNGYDISNYKKIEPQLGTMEDFDMLLKEAHEKGIRIILDLV
                     VNHTSDKHPWFTEAKKGKENLYHDYYIWKDTIPNNWGSSFGGPAWEYVEETAQYYLHL
                     FAKEQPDLNWENPKVRKEVQEILRFWLEKGIDGFRMDVITLISKDPAYPDGPVIQNKA
                     YGSYYAGCASGPHVHEYLQEMNREVLSKYDIMTVGEAPHTSADEAAPYTASDRHELNM
                     VFHFDHMHLDYDENGKYAKNRVKLTDLKEVMTKWQDTMHECDGWNSLYWSNHDQARAV
                     SRFGNESEPYRVKSAKMLGTILHMMQGTPYIYEGEELGMTNAHFESIDEYKDVEALDI
                     FRDFTERKGFSEKDTLELLGLKSRDNARTPMQWDNTVNAGFTEGTPWIGVNKNCKEIN
                     VEQCLADQDSIFYYYQKLIQLRHEMPIITDGVYELLDKENESVYAYLRKGEKETLLVV
                     GNFTDKTINYHIEEKVQAKKSRLLISNYNAAETFERDLSLEPYGACVYLLEAEK"
     gene            6582..7871
                     /locus_tag="ACZPD4_03910"
     CDS             6582..7871
                     /locus_tag="ACZPD4_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1378252.1"
                     /translation="MADYKFDTIQLHAGQERPDPATDARAVPIYATTSYVFHDCADAA
                     ARFGLQAEGNIYGRLTNSTQEVFEKRIAALEGGSAALAVASGAAAITYTLQALAQNGG
                     HIVSQKTIYGGSYNLLAHTLPNLGITASFVDIHDLEAVERAITEQTKALYIETLGNPN
                     CDIPDMDALAELAHKYQIPLVVDNTFGTPYWIRPIEHGADIVVHSATKFIGGHGTTLG
                     GVIVDSGRFDWKASGKYPAITDPNDSYHGVVFSQAAPGAAFVTYIRAVILRDMGAAIS
                     PFAAFLLLQGTETLSLRLERHAENTKKVIEYLNHHPLVEKVNHPLLADHPDHDLYKKF
                     FKNGGGSIFTFEIKGGIKEAHEFIDHLEIFSLLANVADVKSLVIHPATTTHSQLNAQE
                     LQEQGIRQNTIRLSIGTEHIDDIIADLEKGFASVEKL"
     gene            7909..8841
                     /gene="cysK"
                     /locus_tag="ACZPD4_03915"
     CDS             7909..8841
                     /gene="cysK"
                     /locus_tag="ACZPD4_03915"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1378253.1"
                     /translation="MSKIYTSADQLIGKTPLMELTHIEKEHGLKAKILAKLEYFNPAG
                     SVKDRVAKAMIDEAEKSGKLKKDSVIIEPTSGNTGIGLAAVAAARGYRIIIVMPETMS
                     VERRQIMKAYGAELVLSDGARGMKGAIEKAEELAKELPNSFIPGQFVNPANPQAHRDT
                     TGPEIYEDTDAKVDIFVAGVGTGGTLTGVGEYLKSKKKDVEIVAVEPKSSAVLSEGIP
                     GAHKIQGIGAGFVPEILNTNVYDEIIAVSDEDAFATGKMIGQREGILVGISSGAAVWA
                     AIELAKRSENEGKTIVVLLPDTGDRYLSTPMFAD"
     gene            8875..9306
                     /locus_tag="ACZPD4_03920"
     CDS             8875..9306
                     /locus_tag="ACZPD4_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1378254.1"
                     /translation="MLISTKGRYALRVMIDLAQNKESGHVAMKIVAARQGISLKYLER
                     ILPVLTKNGLIEGVHGKGGGYRLCREPKEYKVGEILRLTEGNLAPVSCLECNAPVCER
                     KDQCHTIGMWTDFHKMINDYFDGITLEDLMHRNTELSEDEK"
BASE COUNT         2989 a         1711 c         2204 g         2452 t
ORIGIN      
        1 aaatacttat ataaatatgt agacgcattt ggaaacacaa aatatgtgta tgcttggaga
       61 ttgacaccca cagacccgac accaaaggaa aaacgggaaa aaccctcact tcgtgaactg
      121 gaacagcaga taagacggga tattgaggac ggtatcgaca gcacaggcaa gaaaatgaca
      181 ctttgccaac tttatgccaa acagaacgca cagagagcaa atgtgaagaa aagtacacag
      241 aaacaacggg aacaactcat gcggttattg aaagaggaca agttgggtgc taggagcatt
      301 gatacgataa aaccctctga cgctaaggaa tgggcgttac gcatgaaaga taaaggcttt
      361 tcctataaca ccattaacaa ccataaacgc tcgttaaaag cgtcattcta tatcgccata
      421 caagacgatt gtgtaaggaa gaaccctttt gattttaagt taagtgaagt cctagaaaat
      481 gataccaaag agaaagtcgc attgacagag gaacaggaac aagccttact ctcatttatc
      541 aagacagaca atgtgtatca caagcattat gatgatgtgc tgatactgtt aaagactgga
      601 cttcgtatct cggaactgtg cggactgaca gtagctgata ttgatttcaa gaatgaagtt
      661 gtaattatcg accaccagtt actaaagagc aaggaacagg gctattatat tgaaacgcct
      721 aagacgaaaa gcggaataag acaagtgcca ttaagtagag aaacaataca ggcatttcaa
      781 cgggttatga agaaacgccc aaaggcagaa ccatttgtga tagacggaca gagcaatttc
      841 ttatttgtca atcataaagg caagcccaaa gttgcgattg attacaacgc cttatttgtc
      901 cgtatggtaa agaaatataa caagcaccac aaggacaatc ccttgccaca tatcacaccg
      961 catacgctac gccatacatt ctgcacaaga ctggcaagca agaacatgaa cccaaaagat
     1021 ttacagtata tcatgggaca ttcaaacatt agtatcacaa tgaactggta cgctcatgcg
     1081 tccatagata ccgcaaaatc agaggttcag cgtctaatcg catagaagta tttaccacga
     1141 ttttaaccac gcttgatagc gaaaatataa gaagatagac ctagatatgt gaggtttacc
     1201 acaaaagcaa aatgcccgta gagcctataa aataaggctt tgcggacatt taaggagata
     1261 taaaaagata gtcaaaaaga catatataat ttattttaaa ctttgtcgct cataagatgc
     1321 agacagataa taatgaagga aaatggaaaa caatgaacat ggaagcgtgg attggaccga
     1381 ttctttgttc tttgcagaca ataatcccga cagtgatcgt agtatgcttc ggagctaatg
     1441 tcgtggaact gatcttggaa gttattccgg agtgggtgac aaacggtctt tcgattgcgg
     1501 caggtatgct tcctgtagtc ggtatcggaa tgttgatgag atatatgccg gttaagaaat
     1561 tccttccgtt tattttggtc ggatttgttc tgtccgcata tttgggagta ccggttcttg
     1621 gaattgcgat tataggattt gcggcagcat tttggtattt tacaacagag attaataaag
     1681 cggatgcagt gacgatggca gcagcagaac atgccgatga gatgggagat gattttgatg
     1741 agtaatgaaa tgaacaaaga aatgagtcag ggaacggatg tacagacaga agaacgttat
     1801 caggtgacaa agcgtgatct gagaaaaaca gctgcaagat ataactttat ggcatgcaat
     1861 atttttaatt atgaatcaca gatgggcccg gcaatcgcgt gggcaatggc gcctgtgctt
     1921 agaaagattt ataaaaaaga cgacgaatat aaaatggcat taaataatca ttttaattac
     1981 ttcaactcta cgacagttat gtccagtatg attctcgggg caacgcttgc ggtggaagaa
     2041 aaagatggaa ttgcggcaaa agatacggtt caatctttga aaacgagttt gatgggaccg
     2101 tttgcaggag tgggcgatac tcttgtatgg gtgttgtggc cgacgatcat cggatcgata
     2161 tcaggatata tggcacagca ggggaatcct cttggcgcga ttatttggct tttgtgcaat
     2221 atcgcatttt ggtttgtaaa atcaaagatg ttccagatcg gatatacgtc aggaacaaag
     2281 ttgatcacaa atttaggaaa acgtctgaca gtatttacag aggcggcatc tatcatgggc
     2341 ttaagcgtag tcggagcgct gattgctact tctgtaaaaa taacgacagc tttgaatttt
     2401 aaagtaggcg aagtggcatt gaacttacag acggatattt tggacaagat catgccgtct
     2461 cttcttccgg tcctgttgac tctccttgta tataaattac tttaggcatt tgctaaatgc
     2521 cgcaacccat tgattttact gggttttctc gtttcttata tataaatttt ctctccactc
     2581 atggtaaaat ataagtagcc acacaaacat cccacacatg agaggagaaa aaatttatgg
     2641 ctaataatta tcatcagctt tcacttaaag atactttttc agactgcaag gacatgttta
     2701 tggatgatgt tccttccttt tttcaacttc tggagcagca ttttgatatt tctcttttta
     2761 ttccacaaac tttttataac gctttttatc agcatcttgg tcgcaaaaga gagtatccgc
     2821 ttactggttt cctttctgcc cttatcctac aaaaaatctt tcctatcccc acggattccc
     2881 tgctgatact attattgaat ctttgcaagg aacttcggga tttctgcggt ttttccaaag
     2941 tccctgatgc acctttgttc actcgcttta gactgggctt ttctgaccac attgaactca
     3001 tgtttcagca gatggttgat tataccgaac ccatctgcca gcagattgat tcttcactgg
     3061 ctcagattct tacctttgat acttccggca tcgaactcta tgttacggaa aacaatccca
     3121 aaactctcaa tgcacttatc cgaaaactga aagtttatta caaagatatc cctgatgttg
     3181 acccttataa gatggcttac ggtcttatgc cttcacaggc ggcatcctgt cccgacgcaa
     3241 agcaacaata catcaacgga catttctgtt atgcggataa atttgccatc cttaccaatg
     3301 gtctgggtat catccggcat atctcttttc tggatgatga ctttaaaaca gctcatccgg
     3361 aaatgacgat agaaaagaaa tctgattctc ccgatgaaga taagtccatc ggggatgcaa
     3421 cttccctgaa gcctgtgctt tctgatttct ttttcctgca cccggacttc catccggata
     3481 cttttctggg cgattcttct tttgatacca tcaaaaccta tgggatgctg ttgaatgact
     3541 ttcatttttc caaggctctg attccttaca accttagaaa tgaaagcaat ctcaaaaaag
     3601 tcggatataa cgaatacggc tatccaacct gcccgggcga cccttctctt gccatgaagt
     3661 attgtggtat tacccatgaa aatggacgtg ctgaccgtgt caaatggatt tgtcccaagg
     3721 ttcacatggt aaagggtaaa tatgtctgcg actgtaaaaa tccttgctct accgccacca
     3781 aaggacgcac cacttataca tacgagaatc taaattttcg catgttcccc ggaattcaaa
     3841 gagattccga agaatggaat tccctttaca aaatcagaac cattgtggaa cgtgccatcg
     3901 accatctaaa aatcaacatg tgtgttgccg gaaggaaatc cagaaaccac accaccacaa
     3961 aagcggatgt ttttcttgca gggattgcaa gccagcttac cgtcattgtc gcccacagca
     4021 tgaattgtcc acaatacatc cgaagtttga aacctttaat tgcctaaaaa agtatttcat
     4081 tgtcatagtt catttatggg cttattaaag tacgccccac atctgatgat ttatgtcaat
     4141 tcagaaacca ttctgaataa tgaccgtttt ttctctcctt atatgaggaa agttatccac
     4201 atcaaagaaa aaattattta acaattacct atataaatta cttgggaata aaaaatggac
     4261 agctacaaaa ttgatccttc ttattattgt aatttcactt gtatgtgcgt tttttgggat
     4321 tttaactgta taaaaagtat gatgactgga aaggaaggag aatcatatga aacgaatttt
     4381 gataatagca tctcatgaga agatggcgga tgggatgaaa gatacattga attttatttc
     4441 cggagggata caggaaacga tcgcattgtc agcttatgtg gacaatcgtc cggtagaaga
     4501 agcggttgac gaagtgatgg acggattcga gggggaagat gaagttgttg tattgacgga
     4561 tttaatggtc ggaagtgtaa atcaaaagtt ttttaaatac cgtacgagac cgcatacaca
     4621 tatcgtaagc ggtatgaatc tgcctcttgc atttcaaatt gctatggaac cgcaagggaa
     4681 gtatattacg gaagagagaa tgaaagaaat aattgagacg gcaaaagaag aactgaaata
     4741 cgtaaatgag gtttcaaatg acgaggatga ggaagatgaa taatcagaca gcatggtgga
     4801 aaaaaagcgt ggtatatcaa atatatccga aaagcttcta tgattcggac ggtgatggat
     4861 ttggggactt gaacggaatc agagaaaaac tgtcgtatct tgaaaagctg ggagtagatg
     4921 tgatctggct gaatccgatt tatgaatcac cgcaagttga taatgggtat gatatatcga
     4981 actataaaaa aatagagccg cagcttggga cgatggaaga ttttgacatg ctgctaaaag
     5041 aggctcatga aaagggaatc cgcatcattt tggatcttgt tgtcaatcat acatcggaca
     5101 aacatccgtg gtttacagaa gcaaaaaaag ggaaagaaaa tctatatcat gattattaca
     5161 tatggaaaga tacgatcccg aataactggg gatcaagctt tggcggacct gcatgggagt
     5221 atgtagaaga gacagcgcag tattatcttc atctgtttgc aaaagagcag cctgatctaa
     5281 actgggagaa ccctaaagtc cggaaagaag tacaggagat tcttcgcttt tggctggaaa
     5341 aagggattga cggatttcgg atggatgtaa tcactttgat ttcaaaagat ccggcttatc
     5401 ccgatggtcc ggtgattcaa aacaaagcat acggaagtta ttatgcaggc tgtgcaagcg
     5461 gtccgcacgt ccatgaatat ctacaggaaa tgaatcggga agtgttgagc aaatacgata
     5521 ttatgactgt aggagaagct cctcatacaa gtgcagacga ggcggcgcct tatacagctt
     5581 cggatcggca tgaactgaat atggtctttc attttgatca tatgcatctt gattatgatg
     5641 aaaatggaaa atatgctaaa aaccgcgtga aattgacaga tttaaaagaa gtgatgacga
     5701 aatggcagga tacaatgcat gaatgtgacg gttggaacag tctttactgg agcaaccatg
     5761 atcaggcaag ggcggtatct cgtttcggaa atgaatcaga accgtatcgt gttaaatcgg
     5821 cgaaaatgct gggaacgatc cttcatatga tgcagggaac tccgtatatt tatgagggag
     5881 aagaactggg gatgacaaat gctcattttg agtctataga tgagtacaaa gatgtggaag
     5941 cgcttgatat ttttcgggat tttacggaaa gaaaaggttt ttccgagaaa gatacattgg
     6001 aacttctcgg cttgaaatca agggacaatg cgagaactcc gatgcagtgg gataatacgg
     6061 tcaatgccgg gtttacagaa ggaactccgt ggatcggagt gaataaaaac tgtaaggaga
     6121 tcaatgtgga acagtgtctt gcagatcagg attcgatttt ttactattat caaaaactga
     6181 ttcagcttcg ccatgaaatg ccgattatta cagatggggt atatgagctt ttggataagg
     6241 aaaatgaaag cgtatatgca tatcttagaa agggagaaaa ggagacgctt ctcgtagtcg
     6301 gaaactttac ggataagacg atcaattatc atatagaaga aaaggtgcag gctaagaaaa
     6361 gcagactttt gatttcaaat tataacgcgg cggagacgtt tgaaagagat ttgtcgttag
     6421 agccatatgg ggcatgcgta tatttgctgg aagcagagaa ataaggaaat aatttttcct
     6481 tataatgaaa aaaatgaaaa aactcttgac gaggttggtg ttaagtgata tcttaatacc
     6541 aacattttta gtaggtaaaa aaacagaaag gaagataaga tatggcagat tataaattcg
     6601 acacaattca gttacatgca ggacaggaac gtccggaccc ggcgacagat gcgagagcag
     6661 ttccgatcta tgcaacgaca tcatatgttt ttcacgactg tgccgatgcc gcggcaaggt
     6721 ttggtcttca ggcagaagga aatatttacg gcaggctgac aaactcgacg caggaggtat
     6781 ttgaaaaaag gatcgcggcg ctggaaggcg gttcagcggc tctggcggtt gcttcgggag
     6841 ctgcagcgat cacatatacg cttcaggcat tggcgcagaa cggcggtcac atcgtttcgc
     6901 agaaaacaat ttatggcgga agttataatc tgctggcgca tacgttgccg aatctcggaa
     6961 ttacggcaag ttttgtagat atccatgatc tggaagcggt agaacgtgca attacagagc
     7021 agacaaaagc gctctatatc gagacgctcg gcaatcccaa ctgtgatatt ccggatatgg
     7081 atgcactggc agaattagct cacaaatatc agatccctct tgtggtggat aatacgtttg
     7141 gaacgccgta ttggattcgg ccgatcgaac atggagcgga tattgtcgta cattctgcca
     7201 caaagtttat cggcgggcac ggaacaacac ttggaggcgt tatcgttgat tcgggaagat
     7261 ttgactggaa agcttcggga aagtatccgg ctattacaga cccaaatgac agctatcatg
     7321 gagttgtgtt ttcgcaggcg gctccgggcg cggcgtttgt gacatatatc cgggcggtga
     7381 tcctgcgtga tatgggcgcg gcaatctctc cttttgctgc atttcttttg cttcagggaa
     7441 cagaaacgct ctcccttcgt ttggaacgtc atgccgaaaa tacgaaaaaa gtgatcgaat
     7501 acttgaatca ccatcccctt gttgaaaaag tgaatcatcc gcttctggcg gatcatccgg
     7561 atcatgattt atacaagaag tttttcaaaa atggcggagg ttctattttt acatttgaga
     7621 ttaagggagg aataaaagaa gcgcacgagt ttatcgatca tttggaaata ttttctcttt
     7681 tggcaaatgt tgcggatgtc aaaagtcttg tgatccatcc ggcaacaaca acccattctc
     7741 agcttaacgc acaggaactg caggagcagg gaatccggca gaatacgatc cgtctttcaa
     7801 tcggaacgga acatatcgac gatattatcg cagatttgga aaagggattt gcatctgttg
     7861 aaaaactgtg agaaaataaa attgaaacat gaaaaaggag ataataatat gtctaagatt
     7921 tatacatcag ccgatcagtt gatcggaaaa acaccactta tggaactgac ccatattgaa
     7981 aaagaacatg ggctgaaagc aaaaatcctg gcaaaattgg aatattttaa tccggcaggc
     8041 tctgtaaagg atcgagtagc gaaagcgatg atagatgagg ctgaaaaaag cggaaaatta
     8101 aagaaagatt ctgttattat cgaaccgacg agcgggaata caggaatcgg tcttgcggct
     8161 gttgcagcgg caagaggata ccgcattatt atagtgatgc cggaaacgat gtcggtagaa
     8221 cgcaggcaga tcatgaaagc atatggcgcg gaactcgtgt taagtgacgg agcgagagga
     8281 atgaaaggtg cgattgagaa ggcggaagaa ttggcaaaag aacttccgaa cagctttatc
     8341 ccgggtcagt ttgtaaaccc ggcgaatcca caggctcatc gggacacgac gggcccggag
     8401 atctacgaag atacagatgc gaaggtcgat atctttgtgg cgggagtagg aacaggaggt
     8461 accctgaccg gagtcggaga atatctgaaa tcgaaaaaga aagacgtgga gatcgtggct
     8521 gttgagccga agtcgtcggc agttctttca gaaggaattc cgggagcgca taaaattcag
     8581 ggaatcgggg caggatttgt tccggaaatt ttaaatacaa atgtttatga cgagattatc
     8641 gcagtttcgg acgaagatgc atttgccaca ggaaaaatga tcggacaaag agaaggtatt
     8701 cttgtcggca tttcatccgg agcagcggtt tgggcggcaa tagagcttgc aaaacgcagc
     8761 gagaatgaag gaaagacgat cgtagtgctt ctgcctgata cgggggatcg ctatttgtcg
     8821 acaccgatgt tcgcggattg actttcaaaa agcatgagaa aggagaagtg ctgaatgttg
     8881 atctccacaa aaggaagata tgctttaaga gtgatgatcg atcttgcaca gaataaagag
     8941 tccgggcatg tcgcgatgaa aatcgtggct gcccggcaag gtatttcttt gaagtattta
     9001 gaaaggatct tgccggtgct gacaaaaaac ggattaatag aaggcgttca cggaaaaggc
     9061 ggcgggtatc gtctctgccg tgaaccaaaa gaatataagg tcggagaaat ccttcgtctt
     9121 acggagggaa accttgctcc ggtctcatgc ctggaatgca atgcacctgt ctgtgaaaga
     9181 aaagatcagt gtcacacaat tggaatgtgg actgattttc acaaaatgat caatgattat
     9241 tttgatggta tcacactgga agatttgatg catagaaata cagagttatc agaggatgag
     9301 aagtaatgaa acgaaatatt gatttgaatg aaatatcaga cgggcgtctc tattcg
//
LOCUS       DBMDXF010000063         9225 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2046_length_9225_cov_2.124507, whole genome shotgun sequence.
ACCESSION   DBMDXF010000063 DBMDXF010000000
VERSION     DBMDXF010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2046_length_9225_cov_2.124507"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="ACZPD4_03925"
     CDS             complement(<1..369)
                     /locus_tag="ACZPD4_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="HIR1378255.1"
                     /translation="MSGQYKIMYSSMDQFYDQTNGRMAEWVSQLEPWVKACENLGNME
                     CYQGKSAESVKTYLKEVHMTLLTSIQQAIQLYRTKYLFYREGYYDIEGDLYAVIPQKT
                     LLSVKDRMKTEIEDVSDSSLI"
     gene            complement(463..981)
                     /locus_tag="ACZPD4_03930"
     CDS             complement(463..981)
                     /locus_tag="ACZPD4_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378256.1"
                     /translation="MENKRDDRKKKIREIAGGILCIAVLLFIVMQIMLPETGIGGMVN
                     EGSMAVLFFGVGAVCAYAAIKKRKEDKSALVLGLCLAVLACVPGGWISGKVAADLIEG
                     PKTIRLTSVTTEELNGLSGLISHHYYLCGRGEDGKQYRLEVSADEFYELENIHEIIVR
                     CYSRTERILEFL"
     gene            complement(1048..1365)
                     /locus_tag="ACZPD4_03935"
     CDS             complement(1048..1365)
                     /locus_tag="ACZPD4_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5344 family protein"
                     /protein_id="HIR1378257.1"
                     /translation="MATLQSLGSVAGLINLDYGKFKYAVDQMRTSADGINGNVEERAR
                     GAERSEALDEFAEQFVQLQRIISLYKSLIKKDIDAIETTGEQLKEIDLKLEGVWKGLQ
                     MLK"
     gene            complement(1389..1841)
                     /locus_tag="ACZPD4_03940"
     CDS             complement(1389..1841)
                     /locus_tag="ACZPD4_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 domain-containing protein"
                     /protein_id="HIR1378258.1"
                     /translation="MAENSAEERRKRARVCEARSEKSAREREKAEKESKRAVNEKKIE
                     RLKTARDSIQSQKNSAKAKRKKLEKYANGDEIGEWIGKEQTATVYSIEGNVVGQYNTY
                     IERIDDVVDALCNEITRLENENMQLSWDVLHIGSLINSLVNEIRTLCN"
     gene            complement(1822..2184)
                     /locus_tag="ACZPD4_03945"
     CDS             complement(1822..2184)
                     /locus_tag="ACZPD4_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HIR1378259.1"
                     /translation="MGENVKKETVDYLPLGSVVILKGGVQKVVVIARGLLSVATGKEG
                     YFDYGGCLYPQGLVGDQILYFNHRDIVKVVFSGFHDDDDKMMVDNINQWYMESPYERT
                     DVEKLVRERGAQDGGKLG"
     gene            complement(2391..2684)
                     /locus_tag="ACZPD4_03950"
     CDS             complement(2391..2684)
                     /locus_tag="ACZPD4_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1378260.1"
                     /translation="MASQIRISPDQMRERANSYQTESGNVSEMIGRLDSLLQSLLGEW
                     EGSASEAYRERYESLRPAFTQAEELLAEISKALNTTAQNMENVDESIASGFRG"
     gene            complement(2743..7266)
                     /gene="essC"
                     /locus_tag="ACZPD4_03955"
     CDS             complement(2743..7266)
                     /gene="essC"
                     /locus_tag="ACZPD4_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HIR1378261.1"
                     /translation="MENRYKIILSGNQIYKEAELPADMERVTVGTGIDCTVRLRRDLF
                     FESIQIEFVKESGGWRATCSDNIYFTEGDIRKYMTRKVIHGDTLEVRYQESEGLVFRI
                     DFQIDFDSGSHRCERMINLDRYQTISIGNNSAYEIALSGVYAKREFVRLTRGQGGWTL
                     EVMNSEYGVYHNGKKTEQKEWIKDGDFFSVADYYFFLKGNALWAEIRSDLTVNGLGFG
                     DYPERNGYPRFSRNTRLKTVICEDKIEILDPPSKPQKPKSNLFMKLFPSFGMLIAAGA
                     MAFMGGTMIIFSLISCTIAIITAVVGVMEGKKEFREKTANRIEVYQKYIASKRQEIEE
                     CRNREWTERNEIYIPAEQEIQQVETFSPDLFDRTPQDEDFLCVRLGSGPIESARQVNY
                     KKQEKLEIEDDLSLLPEQTASFYKELQNAPVICDLKNVNAVGITGEEADRFELLKLIV
                     TDVALRHFAADVKLFFVAEKEHAGRMHLFRFLPGAYCVQTDTRGIVTDDESKTLIFEY
                     LYKELTMRAQEKRSYPHLLIFFYDEYGYKKHPISQFTEKGKDLGVTFLFFGQTRADIP
                     VGCDYVVQLSGGYRGVLINAAEKSKTVPFVSSQISDTLAVRIVRTLAPVCTDEVSLEG
                     ELIKNISMFKMLNILSVEDLDLKARWSASKVTKSMAAPVGVSKTGIVMLDLHDKAHGP
                     HGLVAGTTGSGKSEILQTYILSMATLYHPYEAAFVIIDFKGGGMVNQFAQLPHLLGAI
                     TNIDGNAINRSLKSIKAELQKRQKYFAQADVNHIDKYIRKYKAGEVSEPLPHLIIIVD
                     EFAELKAEQPEFMKELISAARIGRSLGVHLILATQKPAGQVNEQIWSNSRFKLCLKVQ
                     SKEDSNEVLKSPLAAEIKEPGRAYLQVGNNEIFELFQSAYSGAPEREDEGTGKAFTIY
                     EVPDGGKKVPVYVKKKNKGQEGMNNQLEAIVSYVSEYFRSIQIPQLPDICLPPLRECI
                     EFPPVSKEAVQEQKKEVGFYAWIGVYDDPDHQNQDQYAVNLSAANMIIIGSAQTGKTT
                     ILQNVIRSLSEQYTPDEVAIYIIDFASMVLKNFETLNHVGGVVSSSEDEKLKNLFKML
                     WEEMETRKEKLLSVGVSSFVAYKEAGRTDMKQIVLIIDNLTALKELYFQDDDELLNLC
                     REGITVGISIVIANAQTAGIGYKYLSSFSNRIALFCNDGNEYSAIFEHCNRRLEHLPG
                     RCFAEVDKQILECQAYLPFAGEKEFERAEAIRGYIEKRNGECTEVARKIPLIPPVLSM
                     NYMAQQFGNMMRGHFDLAAGLNYEDVMPYMLRMSSIGVLAVVGREGSGRYNWIKYVAD
                     MLELMYPGRSKVYISDGIGKKLASMKEKRNVVRYSMIAEDIVSYLKEIEEELQSRYEL
                     LAKGIESDPAEADYMLLIIDNPDAIEQISNSKEALASYKNIIGRYRNMNVGVIISAIE
                     NAPIPYSAPEVIKGIRDGRHLMYFGDISELKIYDMPLAVTRKFKKPIETGDGYYIKEN
                     ECIKLKTPFIAGE"
     gene            complement(7354..7602)
                     /locus_tag="ACZPD4_03960"
     CDS             complement(7354..7602)
                     /locus_tag="ACZPD4_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HIR1378262.1"
                     /translation="METAIIIFHIHKEHRTVDLEVPLDLTAKELAAALNEAYGLGVDL
                     SDINNCYLKAQNPIMLLKGNRTLKEFGVRNGTIISMAE"
     gene            complement(7651..8709)
                     /locus_tag="ACZPD4_03965"
     CDS             complement(7651..8709)
                     /locus_tag="ACZPD4_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1378263.1"
                     /translation="MARPRVIVADTDESYVIPLQKKFAELFADKIDLEIITDKDYFNQ
                     LFSLSQHADILLVSDTLYNSGLKKHDIRHLMILSEHYEPGQTDELDICRLFKYTSVRE
                     IFNEITGRCKETLYAEQDAGKETKIILITSASGGVGKTSVAFGLCRCLERNYKKTLYV
                     NADRLQSFQWMLENGEPIRDASVYAGLSGNLGNVYRIVRHVIRKEGFSYLPPFKAALM
                     SLGIPYRVFALLAEAARKEQEYDYIVIDADSVFDEEKAALMQLADKILIVTKQTEYAV
                     KATNELIQNVNRNNSDKYIFLCNDFKKEEENALLLPTAAHRFTVSEYIEHLPAYEMKG
                     SGLPVQTPGLQKAAFLIM"
     gene            complement(8725..9150)
                     /locus_tag="ACZPD4_03970"
     CDS             complement(8725..9150)
                     /locus_tag="ACZPD4_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012708.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1378264.1"
                     /translation="MLRSAGVSSEDSDVEKCGSVYETSTPYSLNRNEKVSSQRGDQTC
                     TLVSLTAGSPIRLTVTKQEYVIGKSTEQADGVAGFSKMISRRHCKIVKRGSGYAVVDL
                     NSSNGTYLNGMQLFPGREYPVKNGDIIRMAISDFQIVME"
     gene            9161..>9225
                     /locus_tag="ACZPD4_03975"
     CDS             9161..>9225
                     /locus_tag="ACZPD4_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378265.1"
                     /translation="MALGKTTKEIAVERNLSFHTI"
BASE COUNT         2278 a         2451 c         1542 g         2954 t
ORIGIN      
        1 tattagagaa gaatcagata cgtcttcgat ctctgttttc atccggtctt ttacagaaag
       61 taacgtcttc tggggaatga ctgcatataa gtccccttct atatcataat atccttcacg
      121 gtaaaaaaga tattttgtcc tatataactg tattgcctgc tgtatagaag taagcagcgt
      181 catatgcact tctttcagat acgttttaac agactccgcg ctttttcctt gataacattc
      241 catatttcca agattttcac acgccttaac ccacggttct aactgactga cccattctgc
      301 catgcgcccg tttgtctgat cataaaattg atccatagaa ctatacatta ttttatattg
      361 tccactcata atttttcctc tgatatttca tcaaaatctc tgtacaatgt ctgcatatcg
      421 aaaaactatc ccgaaaaatt ccgtaacagt tcattttaac tattacaaaa attccaaaat
      481 ccgctctgtt ctcgaataac atcgtacaat gatttcatgt atattttcca actcataaaa
      541 ctcatctgcc gatacttcca gccgatactg ctttccgtct tcccctctgc cgcataaata
      601 atagtgatga gaaattaacc ctgataatcc gttgagttcc tctgttgtaa ccgatgtcag
      661 acggatcgtt tttggtcctt ctataagatc tgctgcgacc ttcccagaga tccagccacc
      721 gggaacacat gctaaaactg ctaagcataa accgagaaca agcgcagatt tatcttcttt
      781 tcttttcttg atagctgcat aagcacagac tgctcctact ccgaaaaata aaactgccat
      841 tgacccttcg ttcaccatcc cgcctatgcc tgtttccggc agcataattt gcataacaat
      901 aaaaagcaaa actgcaatac acaaaattcc tccggcaatt tcccgaattt tcttttttct
      961 atcatctcgt ttattttcca cgttcactct ttttgctttc cttttttatt tttattttct
     1021 catgtatttc tgctgttctc aatcacttta tttcaacatt tgaagacctt tccatacacc
     1081 ctctaatttt aaatctattt ctttcagttg ttctcccgta gtttcaatcg catcaatatc
     1141 tttttttata agtgatttat aaagacttat gattctttgc agctgaacga actgctctgc
     1201 aaactcatca agagcttctg atcgctcagc tccccgcgct ctttcttcta cattcccgtt
     1261 aattccgtcg gcgcttgttc tcatctggtc gacagcatac ttaaatttcc catagtcgag
     1321 attgatcaga cctgctaccg atcccaacga ttgcaacgtt gccatagtga tacctcctcg
     1381 tataatactt aattgcaaag tgtacggatc tcattgacaa gagaattgat caaagatcct
     1441 atatgaagca catcccaact caactgcatg ttttcatttt ccagccttgt tatttcattg
     1501 caaagcgcgt ctacaacatc gtcaatacgc tcaatataag tattgtactg tccgacaacg
     1561 tttccttcta tactatatac tgtcgccgtc tgttccttcc cgatccattc tccgatttca
     1621 tcaccgtttg catatttttc cagttttttc cgctttgctt ttgccgaatt tttctgcgac
     1681 tggatactgt ctctcgccgt ttttaatctt tcgatctttt tttcattcac ggcgcgcttt
     1741 gattcttttt ctgctttctc ccgttcacgc gcgctttttt cgctgcgcgc ctcacacact
     1801 cttgcccttt ttcttctttc ctcagccgag ttttccgcca tcttgagcac cccgctctct
     1861 tacaagtttt tctacgtctg tgcgttcata tggtgattcc atataccact gattgatatt
     1921 gtctaccatc atcttatcat cgtcatcgtg aaatccgctg aaaacaactt ttacgatatc
     1981 tctgtgatta aaatataaga tctgatctcc gaccagtcct tgcgggtaca gacatccgcc
     2041 gtaatcaaaa taaccttctt tccctgtcgc aaccgacaaa agccctctgg caatcacaac
     2101 aactttctgc acgcctccct ttaagatcac aacacttcca agcggaagat aatctaccgt
     2161 ctcttttttt acgttttctc ccatcatgct tttcctttct gtttcaattc atttaaggta
     2221 accgctcagt ccgcgccatt cgtaaagccg cgctgcgtac ttatcatggc taacgctgct
     2281 gtacggctga actgttacta tttaagcttt ttaagttccg catttcccac aaaaggggaa
     2341 atgcggaacg cggttttatc cgccgctgcc tttaaacctg ctttttcgta ctatcctctg
     2401 aatccgcttg caatactttc gtctacattt tccatatttt gagctgtcgt attcaacgct
     2461 tttgaaatct ccgcaagcaa ttcttctgcc tgcgtaaacg ccggccgcag actttcataa
     2521 cgttctctgt aagcctcgct tgccgatcct tcccattctc ctaaaagaga ctgaagtaac
     2581 gaatctaatc ttccgatcat ctcgctgaca ttccctgatt ctgtttggta cgaatttgcc
     2641 ctttctctca tctgatccgg tgaaattctg atttgactag ccataattgc tacctcctat
     2701 aaaatttttt atttatatat accggtcttc cggatatata tcctactctc ccgcaatgaa
     2761 cggggtcttc aatttaatac actcattttc ttttatgtaa tatccgtcgc ctgtctcgat
     2821 cggttttttg aactttcttg tcacggcgag cggcatatcg taaatcttca attctgaaat
     2881 atctccgaaa tacatcagat gtcttccgtc ccggatgcct ttgatcacct ccggcgcgct
     2941 gtatgggatc ggcgcatttt cgattgccga tataatcacg cctacattca tgttccggta
     3001 gcgtccgata atatttttat acgatgcaag cgcctcttta ctattgctga tctgctcgat
     3061 cgcatcagga ttatcgataa taagaagcat gtaatctgct tctgccggat cgctttcgat
     3121 cccttttgca agaagttcat atctgctttg cagttcttct tcgatctctt tcagataaga
     3181 aacgatatct tccgcgatca tgctgtaacg aacgacattt cttttttctt tcatggaagc
     3241 aagcttcttc ccgattccgt ccgaaatata aacttttgat ctgcccggat acatcaattc
     3301 cagcatatcc gccacatatt ttatccagtt atagcgtccg ctgccctctc ttccgacaac
     3361 cgccaataca ccaatcgaag acatacgaag catatacggc atcacatctt cgtaatttaa
     3421 tcctgccgcc aggtcgaaat gtccgcgcat catatttcca aactgctgtg ccatataatt
     3481 catgctcaaa acaggcggga tcaacgggat cttccgagcc acctcagtac attctccgtt
     3541 tcgtttttcg atatatccgc ggattgcttc cgctctttca aattcttttt ctcctgcaaa
     3601 cggcagatac gcctgacatt caagtatctg tttatccact tctgcgaaac atcttcccgg
     3661 aagatgctca agtctcctgt tacaatgttc aaaaatcgca gagtattcgt ttccgtcatt
     3721 gcagaataac gcgatcctgt tagaaaaaga cgacaaatat ttgtatccga tccctgccgt
     3781 ctgtgcattc gcgatcacaa tagaaattcc gactgtaatt ccttcccgac acagattcag
     3841 cagttcgtca tcatcttgaa aatacaattc tttcagcgca gtaagattat ctataataag
     3901 aacaatctgc ttcatatcgg tgcgccccgc ctctttgtac gcaacaaatg agcttactcc
     3961 gacagataat agtttctctt ttcgagtttc catctcttcc caaagcattt taaacagatt
     4021 tttcagtttc tcatcttcgg aggaactgac aacgccgcct acatgattga gcgtctcaaa
     4081 gtttttcaat accatagacg caaagtctat aatgtagatc gccacctcat ccggcgtata
     4141 ctgttccgaa agacttcgga tcacattttg cagaatcgtc gtctttccgg tctgcgccga
     4201 accgataatg atcatatttg ccgcgcttaa attcactgca tactgatcct gattctggtg
     4261 atcgggatcg tcataaacgc cgatccacgc ataaaatccg acttcttttt tctgctcttg
     4321 tactgcctct tttgataccg gcgggaattc gatacactct ctaagcggag gcagacagat
     4381 atcgggaagc tgcggtattt gaatcgaacg gaaatattcg gacacataag aaacgatcgc
     4441 ctcaagctga ttgttcatcc cttcctgtcc tttgttcttt ttctttacat aaaccggaac
     4501 ttttttcccg ccgtccggaa cttcatatat cgtaaatgct tttcctgttc cttcgtcttc
     4561 tctttccggc gctccgctgt atgccgactg gaacaattca aatatctcat tgtttcctac
     4621 ttgcagataa gctcgtccgg gttctttgat ttctgcggca agaggagatt tcagcacttc
     4681 gttactgtct tctttgctct gcactttcag acagagttta aaacgggagt tactccatat
     4741 ctgttcattg acctgacctg caggtttttg agtcgccaga atcagatgta ctccaagact
     4801 tcttccgata cgcgctgccg agatcagctc tttcataaat tccggctgtt ccgctttcag
     4861 ctctgcaaat tcatctacaa tgatgatcag atgcggaagc ggttcgctta cttcgcccgc
     4921 tttatatttt cggatatatt tatcgatatg gttgacgtcc gcctgtgcga aatatttctg
     4981 tctcttctgc aattctgctt tgatcgactt aagcgaccga ttgatggcat tcccatcaat
     5041 atttgtgatc gcgccaagca gatggggcag ctgcgcgaac tgattcacca tacctccgcc
     5101 cttgaagtca atgatgacaa acgccgcttc gtaaggatga tagagcgttg ccatcgaaag
     5161 gatatacgtc tgcaatatct ctgattttcc agatcctgtc gttcccgcaa caagaccgtg
     5221 cggaccatga gctttgtcat gaaggtcaag cataacgatc cctgtctttg agactccgac
     5281 cggagctgcc attgacttcg taactttaga cgcagaccat cttgctttga gatcaagatc
     5341 ttccacagac aaaatattca gcatcttaaa cattgatata ttttttatca gttcaccttc
     5401 caatgaaact tcatccgtac aaaccggagc taacgtcctg acaatgcgta ctgccaaagt
     5461 gtcgctgatc tgagaagata caaacggtac tgtctttgat ttctccgctg cgttgatcaa
     5521 aacgcctctg taaccgccgg acaactgtac gacatagtcg catcctaccg gaatgtccgc
     5581 gcgtgtctgt ccgaaaaaca aaaaagtgac tcccagatct tttccttttt ctgtaaactg
     5641 tgagatcgga tgcttcttat atccgtattc gtcatagaaa aagatgagca gatgcggata
     5701 cgatctcttc tcctgtgctc tcattgtcag ctctttgtac aaatactcaa aaataagcgt
     5761 cttgctctca tcgtctgtta caattcctct cgtatctgtc tgtacgcagt acgctcccgg
     5821 aagaaaacgg aacaagtgca tccgtccggc atgttctttt tccgccacga aaaacaattt
     5881 tacatctgcc gcaaaatgac gaagcgccac atctgtaacg atcagtttca aaagttcaaa
     5941 ccgatctgcc tcttctcctg taatgccgac tgcatttaca tttttcagat cacatatgac
     6001 cggcgcattt tgaagttctt tataaaaact cgccgtctgc tcaggaagaa gacttaaatc
     6061 gtcttcaatt tccagcttct cctgtttctt gtaatttacc tgacgggcag actcgatcgg
     6121 accgcttccg agacggacac aaagaaaatc ttcatcctgc ggcgtccggt caaacagatc
     6181 cggagaaaat gtttccacct gctgaatctc ctgctctgcc ggaatataga tttcattacg
     6241 ttctgtccat tctctatttc tgcactcttc gatttcctga cgttttgacg caatatattt
     6301 ctgatatact tcgatccggt tcgccgtctt ttcccgaaac tctttctttc cctccatcac
     6361 accgactacc gctgttatga tcgcgatcgt acatgatata agagaaaaaa taatcatcgt
     6421 tcctcccatg aacgccatcg cgcctgccgc gatcagcatt ccgaaggacg gaaataattt
     6481 cataaataaa ttacttttcg gtttctgcgg ctttgaaggc ggatctaaaa tctcgatctt
     6541 gtcctcacat atcaccgttt tcagccgtgt atttctcgaa aatctcggat acccgtttct
     6601 ttccggataa tccccgaaac caagtccgtt cactgtcaga tcgcttctga tctccgccca
     6661 cagtgcgttc ccttttaaga aaaagtaata atctgcaacg gaaaagaaat ccccgtcttt
     6721 gatccattct ttttgttctg tcttcttccc gttgtgatat actccgtact cactgttcat
     6781 aacttcaagc gtccagccgc cttgtccccg cgtcagccgg acaaattctc tctttgcata
     6841 aacgcctgat agtgcaattt catatgctga gttgtttccg attgaaatcg tctgatacct
     6901 gtccaaattg atcatccgct cacagcgatg cgatccgctg tcaaaatcga tctgaaaatc
     6961 aattctaaat acaagtcctt ctgattcctg atacctgact tccaaagtat ctccgtggat
     7021 cactttccgc gtcatatatt ttcggatatc gccttccgta aaataaatgt tgtccgaaca
     7081 tgtcgctctc catccaccgc tttctttcac aaattcaatc tgtatcgatt caaaaaacag
     7141 atctcttctc aaacgcaccg tacagtcgat tcccgttcct accgtaaccc gttccatatc
     7201 agccggaagt tctgcttctt tataaatctg atttcccgaa agtataatct tatatctgtt
     7261 ctccatatct ttcctctgcc gtttcccgct ccttatctgc cgctgccttt gccgccggca
     7321 gacttatctg ataagattct tctcttttgc gccttactct gccatgctta tgatcgttcc
     7381 gtttcttact ccgaattctt ttaacgttct gttgcctttg agcagcatga tcggattctg
     7441 cgcctttaga taacaattat ttatatctga cagatcgacg cccagaccat acgcctcgtt
     7501 aagcgccgcc gccagttctt ttgccgtcag atccagcgga acttctaaat ctaccgtccg
     7561 atgttcctta tgaatatgaa aaataataat cgccgtttcc atcttctctc accacctgca
     7621 ctcatatcgt ctttgtattg atttttcttt ttacatgatc aaaaatgccg ctttctgcaa
     7681 tcccggcgtc tggacaggca gtcccgatcc tttcatttca tacgccggaa gatgttcgat
     7741 atattcgctc accgtaaatc gatgcgccgc tgtcggaagc agcaatgcat tttcttcctc
     7801 tttcttaaaa tcattacaaa gaaatatata tttatcacta ttgttccgat ttacattttg
     7861 gatcagctca ttcgttgcct tcaccgcata ttccgtctgt tttgtcacga tcaatatttt
     7921 atccgcaagc tgcataagag ccgctttttc ctcgtcaaac acactgtctg catcgataac
     7981 gatgtaatcg tattcctgct ctttgcgggc tgcttctgct aatagtgcaa acacgcggta
     8041 tgggataccc aacgacataa gcgccgcttt aaacggcgga agataactga aaccttcctt
     8101 tcgtatgaca tgccgtacga tccgataaac attgccaaga ttccccgaca gacccgcata
     8161 aaccgaagca tctctgatcg gctcgccgtt ttccagcatc cactgaaacg actgaagccg
     8221 atccgcattc acatacagtg ttttcttata attacgttcc agacaacgac acaagccgaa
     8281 tgcaacactc gttttcccga ctccgcctga cgccgaggtg atcaatatga tctttgtttc
     8341 ttttccggca tcctgttcgg cataaagcgt ctccttgcat ctgcctgtaa tttcattaaa
     8401 aatctcccgc acacttgtat atttaaacaa gcggcaaatg tcgagttcgt ccgtctgtcc
     8461 gggttcgtaa tgttccgaca atatcatcaa atgccggata tcatgttttt tcagcccgct
     8521 gttataaagc gtatccgaca caagcagaat gtccgcatgc tgcgaaagtg aaaataactg
     8581 attaaaataa tctttatccg taatgatctc caagtcgatc ttatccgcga acagctctgc
     8641 aaatttcttt tgtagcggaa ttacatagct ttcatctgta tccgccacaa tcactctcgg
     8701 tcttgccata tttctctgtc acctctattc catcacgatc tggaaatcgc tgatcgccat
     8761 tcgtataata tctccgtttt tcaccggata ttctctgccc ggaaaaagct gcatcccatt
     8821 caagtatgtt ccgtttgaac tattcagatc tacaaccgca tacccggatc ccctttttac
     8881 gatcttacag tgccttctgc tgatcatctt gctgaatccg gcaacgccgt ccgcttgctc
     8941 cgtactcttt ccgatcacat actcttgttt cgttacagtc agacggatcg gactgcctgc
     9001 tgtcagcgac acaagtgtac aagtctgatc tcctctctgt gaagaaactt tttcatttct
     9061 attcaaacta taaggtgtac ttgtttcata tactgatccg cacttttcca catccgaatc
     9121 ttcactgctg acaccggccg aacgaagcat cctgaaagaa atcgcattag gaaaaaccac
     9181 taaagaaatt gcagttgaaa gaaacctcag ctttcatacc ataaa
//
LOCUS       DBMDXF010000064         9208 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2051_length_9208_cov_1.959262, whole genome shotgun sequence.
ACCESSION   DBMDXF010000064 DBMDXF010000000
VERSION     DBMDXF010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2051_length_9208_cov_1.959262"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="ACZPD4_03980"
     CDS             <1..690
                     /locus_tag="ACZPD4_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR1378266.1"
                     /translation="VWYDALDHDIPLNQSIMNEIADTVGSISANRKLKNADEAKDYGL
                     DVPIYTIEYTDDNDDVISVYFGNNTGDNIYATLKGEKSIYTVSSQVIEDLNYTEEDLI
                     QLDDYPSIGSGNLEKAVITKNKNSVVYDSADETQTEQIIAIAGGLGAVQLSTTADYCA
                     EEKELSEYGLDEDLRAAVEVTYQEDEKEKKLTLYIGNRVGDDRYVMLNDSKIVYLVSD
                     AICGNILNEEE"
     gene            802..1209
                     /locus_tag="ACZPD4_03985"
     CDS             802..1209
                     /locus_tag="ACZPD4_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1378267.1"
                     /translation="MKEKLIRFMQGRYGIDQLSKFLLITGLAVVLLSAFFGETAVSML
                     LYIFGWGVVIYCYFRIFSRNVQKRYAENQAYLMKTYKIRNWFQNQKNIWQQRKVYHIY
                     TCPSCKQKIRIPKGKGKIEVRCPKCGTTFIKKS"
     gene            complement(1344..2408)
                     /locus_tag="ACZPD4_03990"
     CDS             complement(1344..2408)
                     /locus_tag="ACZPD4_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR1378268.1"
                     /translation="MKFYKKRWIQSGIILISSLGIVLSVFPSFADDNIDNLKGQTSAL
                     ESELDGINKDILSLSEEISATEMQVEILNGEIARTSDALVEAEENENEQYENMKSRIK
                     YMYEHGNATLLEMLFSAENMADFINKADFIENLSEYDKTALDDLKNIYKNIEDQKNTL
                     ETQQDSLLDLKDELKKKQTELQEKAKATSTNLTEVQAKLEKAKAEEAARLEAERLEQL
                     QQNGGYDDTITNNGGQIDASADDVTLLAAILQCEAHQNYDSLLAVATVILNRVESPRF
                     PNTIRGVIYASGQFEPVWTGRLDAVLKKGPTSLSMQVAQDAINGARLAAVADCYYFLY
                     AGSTNRPGVNIGNNLFFPSW"
     gene            2561..3409
                     /locus_tag="ACZPD4_03995"
     CDS             2561..3409
                     /locus_tag="ACZPD4_03995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1378269.1"
                     /translation="MTHKIKMIGLDLDGTLLTDKKELTVRTREVLAKAIRSGIVVLVA
                     TGRPWMGVPEELREFPGMDYALTSNGARIIDTRTGNVIEEHLLSVKSAKKALEICRKY
                     DTLQEVYFDGQGYAPAEKMEFVERYHKNPNMWEYMRKTRIPVDDIFELVDRENRGLDK
                     TQALFADMSERKHAWEELARHEDLELVGSLRYNIEVNAAGVNKGTGLVNLGSRLGIKR
                     EEIMAFGDGDNDTVMLKEVGFGVAMANGEPQVKEAADYITLSNEEDGVAQAIERFVLG
                     GGEARC"
     gene            3403..4230
                     /locus_tag="ACZPD4_04000"
     CDS             3403..4230
                     /locus_tag="ACZPD4_04000"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1378270.1"
                     /translation="MLEALKVIIFGIVEGITEWLPISSTGHLILLEELLHLKQNQAFL
                     DMFYVVIQLGAILAVVVMYFHKLNPFSPKKTQKQKMLTIQLWVKVIIGSIPAMLIGLP
                     FNDFIEEKFNNSYVVAATLIIYGILFIVIENYQKDKQPKVEKLSQLTIQTVLIIGVFQ
                     ALALIPGTSRSGATIVGALLIGVSRGVAAEFTFFLAIPAMFGASFLKLIKFGLNFTGM
                     QVFLLLLGTVVSFAVSILAIKFLMQYIRKHDFKVFGYYRIVLGVIVLLYFGIKTLLA"
     gene            4446..5336
                     /locus_tag="ACZPD4_04005"
     CDS             4446..5336
                     /locus_tag="ACZPD4_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1378271.1"
                     /translation="MKNTKMKKFLAVVLSAALVAGLAAGCGSKEDGKEEDKGSSAKTA
                     KVIDVDLTNEEYAFGIDKDQPELVEQVNAFIKEVKEDGTLDEICDKYFGGGEPAAVES
                     AKLDESKDQLVVATNAAFEPFEYTKGDKYVGIDMELAALLAEKLGKELVIQNMDFDAV
                     CLSVGQHKCDIAIAGLTVNDERKQYVTFSDTYYQASQRLIVPSDDDTFDDLKDADAVA
                     AKLGELKKEDKIGVQQGTTAQSYIEGDEAFGFEGLPATCQPYKSGSLAVQDMLNGNIK
                     YVIIDAAPATAITEAINAMQ"
     gene            5562..6272
                     /locus_tag="ACZPD4_04010"
     CDS             5562..6272
                     /locus_tag="ACZPD4_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1378272.1"
                     /translation="MGNFSAKVDKFIEIFIDQNGYVKVVQGLQNTVLIAIVGLVIGIL
                     IGTVIATARVLPKYKVLPRVLNGICSVYVAMFRGTPMVVQLLVFYYVLLPIIGWNITG
                     VQVAMLVFGLNSGAYISEIMRSGIQSVDAGQMEAGRAVGLSFGASMMKIVIPQAVKNI
                     LPTLGNEFIALIKETSVVSFVGAADLYVAFNYIGTNSYEFMVPYLVMALIYIALILVV
                     TLLVKLLERSLKKSDRRN"
     gene            6256..7080
                     /locus_tag="ACZPD4_04015"
     CDS             6256..7080
                     /locus_tag="ACZPD4_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1378273.1"
                     /translation="MIDVINLRKSFGDLEILKGINITINKGDIVAVLGPSGSGKSTFL
                     RCLNCMEDPTSGSIVFKGVDIADMKVDINVHRRHMGMVFQHFNLFNNKTVLQNVMMAP
                     AYLQCKDLKKAKNKNRMIRFKNIFRSKKEELIPITTTKEQIKSETKKQALELLGRIGL
                     EDKADVYPSTLSGGQKQRVAIIRSLAMNPDVILFDEPTSALDPEMVGEVLELMKKLAQ
                     EGMTMVVVTHEMGFAKEVASRVIFMDDGQIVEEAEPEEFFGNPKNPRLKEFLSKIL"
     gene            7229..9022
                     /gene="mutY"
                     /locus_tag="ACZPD4_04020"
     CDS             7229..9022
                     /gene="mutY"
                     /locus_tag="ACZPD4_04020"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1378274.1"
                     /translation="MRYDRIYKAKFIERPNRFIAYAELNGKKETVHVKNTGRCAELLR
                     PGADIYIQESDNPSRKTRWDLIAVKKGSRMINMDSQIPNKVVKEWIEQGNLFGNVTLI
                     RPETVYKNSRFDLYVEAEDQNGGIRKIFIEIKGVTLEENGVCRFPDAPSERAVKHLEE
                     LSDAKKNGYEAYVFFVIQMKGVRYFTPNTETHPQFAEALQKTAKEGVKVLAYDCDVTS
                     DAIELSDAVDVVLGNPILKESVRPLVEWFRENKRDLPWRKRINAYRVWISEIMLQQTR
                     VEAVKPYYERFLSELPDVSALASVEEDRLLKLWEGLGYYNRARNLKAAACQIMEQYGG
                     RFPSSYEEIRSLKGIGNYTAGAIGSFVYHIPKPAVDGNVLRVVSRLAADEGDIKTAAV
                     RSKVEELIEEIIPKDAPGDFNQGLIELGAIVCVPNGEPKCAACPLEALCKAHKEGREM
                     DFPVKKKAKARRIEKRTVFIFRDNEKVAIRKRPQKGLLAGMYEFPNVEGHLRTEEVIG
                     YAQTAGLTPVRVKRIGTAKHIFSHVEWHMTGYELLVDELEKTSAPNVGQMCVENGADG
                     SENIFVKIKQLEEEYAMPSAFDKFVEHTRFS"
BASE COUNT         2893 a         1536 c         2340 g         2439 t
ORIGIN      
        1 gtgtggtatg atgctttaga tcatgatatt ccgcttaatc aaagcattat gaatgaaatt
       61 gcagatacgg ttggcagtat ttctgcaaat cggaagttga aaaatgcaga tgaagcaaaa
      121 gactatggtt tggatgttcc tatttatacg attgagtata cagatgataa tgatgatgtc
      181 atttctgttt atttcggaaa taatacaggg gacaatattt atgcaacatt aaaaggggaa
      241 aaatctattt atactgtttc gagtcaggtc attgaagatt taaattatac agaagaagat
      301 ctgatacaac ttgacgacta tccatcgatt ggaagtggca atcttgagaa ggcagttatt
      361 acgaaaaata aaaattctgt tgtatatgac agtgcagacg agacgcagac ggaacaaata
      421 atcgcaatag ccggaggcct tggagctgtg cagctgtcca caacggcaga ttattgtgca
      481 gaagaaaaag aattatcaga atacggtttg gatgaagatt taagggctgc ggtagaagtg
      541 acttatcagg aagatgaaaa agaaaagaaa ctgacattat atataggaaa ccgggtcggg
      601 gatgacagat atgtgatgtt aaatgattca aagattgtgt atctggtttc tgatgcaata
      661 tgtggaaata tattaaatga agaagaataa aaacaggtta taagcagtaa gattttatat
      721 tcttttaatt gactttgcag gagaaacgag agagaatgtt attcatatca aaaaggcatt
      781 aatgcagaag gatggatgga aatgaaagaa aaattaatac gttttatgca gggcagatac
      841 ggtatcgatc aattatcgaa gtttcttctg atcacgggat tggcggtagt tcttttatcg
      901 gcgtttttcg gagaaaccgc ggtgagcatg cttttatata ttttcggctg gggagtcgtg
      961 atctactgtt attttcggat attttcaaga aatgtacaga agcggtacgc agaaaatcag
     1021 gcatatctta tgaaaacata caagataaga aattggtttc agaatcaaaa aaacatctgg
     1081 cagcagagaa aagtgtacca catttataca tgtccgtcat gtaaacaaaa aatccgcatc
     1141 ccgaagggca aagggaaaat tgaagtaaga tgtccgaagt gcggtacgac ttttataaag
     1201 aaaagttaag ctgtgcagac aacgaaaata tagaactgaa aagtaagaaa tccccgtaac
     1261 aatgaactgc tccaaaaagt ctgacttttt tggaagagtt cgttgttacg gggattttgt
     1321 tttcggtttc aataacgata tctttaccag ctagggaaga aaaggttatt tccgatgttg
     1381 actcccggtc ggtttgtaga tcctgcatag aggaaataat aacagtctgc cactgccgca
     1441 agtcttgcgc cgttgatcgc atcttgcgca acctgcatag ataaagaggt cggacctttc
     1501 ttcagaacag cgtcaagacg acctgtccat accggttcaa actgtccgct tgcatagata
     1561 actcctctga tcgtattcgg aaaacgagga ctttccacac ggtttaatat tacagttgcg
     1621 acagcaagca gagaatcata gttctgatga gcttcacact gcaggatcgc cgcaagaagt
     1681 gtgacgtcat ccgcagaagc gtcgatctgt ccgccgttgt ttgtaatagt atcgtcgtag
     1741 ccgccgtttt gctggagctg ctccagtcgc tctgcctcaa ggcgggcagc ctcttcagcc
     1801 tttgcttttt ctaatttggc ttgtacttcc gtaagatttg ttgaagtcgc ctttgctttt
     1861 tcctggagtt ccgtctgttt tttcttaagt tcatctttca gatcaagaag tgaatcctgc
     1921 tgagtttcta atgtgttttt ctgatcttcg atatttttat aaatattttt cagatcgtca
     1981 agggcagttt tgtcatattc gctcagattt tcgataaaat cagctttgtt tataaaatct
     2041 gccatgtttt ccgccgaaaa gagcatctca agcagtgttg cgttcccgtg ttcgtacata
     2101 tatttaatac gggatttcat attttcatac tgctcatttt cgttttcttc agcttcgaca
     2161 agtgcatcgg aagtacgggc aatctcaccg tttaagatct caacctgcat ttctgttgca
     2221 gagatctctt cgctcaaaga gagaatatct ttgttgatac cgtctaattc actttcgagt
     2281 gcagaagtct gtccttttaa attgtcaatg ttgtcatctg caaatgaagg aaaaactgaa
     2341 agtacaattc cgagagaact gatcaggatg atgcctgatt gtatccaccg ttttttgtag
     2401 aatttcatag ctttaaattc ctttcttgaa gcatttttta agtgctgaat aatcattata
     2461 ctacaaattg cgttatttgg caaactttga tatgataaag gctgagaagg ccgctttcaa
     2521 aagcagcgaa agacagaacg gaaaagaaag aggtatatct atgacgcata agataaaaat
     2581 gatcggactt gacctggacg gaacattgct gacggataaa aaagaactga ctgtgcggac
     2641 aagagaagtt cttgcaaagg cgatcagaag cggaatcgtc gtacttgtag ctacggggcg
     2701 tccgtggatg ggagttccgg aagaattgag agaatttccg ggtatggatt atgcgctgac
     2761 gtctaatgga gcaaggatta tcgacactcg tacgggaaat gtaatagaag aacatctgtt
     2821 gtctgtcaaa tcagcgaaaa aggcgttgga aatttgtcga aaatatgata ctctacaaga
     2881 ggtatatttt gatggacagg gatatgctcc ggcagaaaaa atggagtttg tggaacgata
     2941 tcacaaaaat ccgaatatgt gggaatatat gcgtaaaacc cgtattccgg tggatgatat
     3001 ttttgaactg gtagacagag aaaacagagg tctcgataaa acgcaggcat tatttgcgga
     3061 tatgtccgag agaaaacatg cgtgggaaga acttgcccgt catgaggatt tggaactggt
     3121 cggatctctt cgttataata tagaagtgaa cgcggcggga gtcaataaag gaacaggact
     3181 tgtcaatctc ggaagcaggc ttggtattaa gcgggaagag atcatggcgt tcggagacgg
     3241 agataacgat accgtgatgc tgaaagaagt cggtttcggc gtcgcaatgg caaacggaga
     3301 gccgcaggtg aaagaagctg cagattatat cacactgtca aatgaagagg acggcgttgc
     3361 gcaagcgatt gaaagatttg ttctcggagg aggagaagca agatgttaga agcattaaaa
     3421 gtaattatat tcggtattgt ggagggaatc acggaatggc tgccgatcag cagcacgggg
     3481 catctgatct tgttggaaga attactgcat ttgaaacaaa atcaggcgtt tcttgacatg
     3541 ttttatgttg tgatccagct tggagcgata ctggcggttg tcgttatgta tttccataag
     3601 ctgaatccgt tttcaccgaa gaaaacccag aaacagaaga tgttgacgat ccagttgtgg
     3661 gtcaaagtga tcataggttc gattccggca atgctgatcg ggcttccgtt caatgatttt
     3721 atagaagaaa agttcaacaa ttcatatgtt gttgccgcga cattgatcat ctacggtatt
     3781 ctgtttatcg taatcgaaaa ctatcagaaa gataagcagc cgaaagtgga aaaactttct
     3841 cagcttacga tacagacggt tttgatcatc ggcgtatttc aggcattggc gctgatcccc
     3901 ggaacatccc gttcgggagc gacgatcgtg ggagcgcttc tcataggagt atcaagagga
     3961 gttgcggcgg aatttacatt tttccttgca attccggcga tgttcggcgc aagttttttg
     4021 aaactgatta aattcggatt aaatttcacg ggtatgcagg tatttctgct tttgttggga
     4081 acggtcgttt cgtttgcggt ttccatcctt gcgatcaagt tcctcatgca gtatatcaga
     4141 aaacatgatt ttaaagtgtt tggttactac agaatcgtac tgggagtcat tgtgcttctt
     4201 tacttcggta ttaagacgct gcttgcataa gaattgtttc ggcgaaaatg tgtattttgc
     4261 aaaaaacctg tataaagaat gaagatttat gcataaagta acgcataata tacataaaaa
     4321 ctgaaacaga atgcataaaa ttaaaaaaag agcttgattt ttatgcagaa cgtgttagaa
     4381 tgtagtgcat ggaatgcatg gttattcagc attgagcaat ggaattataa acggagggtg
     4441 cgaaaatgaa aaatacgaaa atgaaaaagt ttttagcagt agttttaagt gcggcgcttg
     4501 ttgccggact ggcagcaggt tgcggaagca aagaagacgg taaagaagaa gataaaggaa
     4561 gcagcgcgaa aacggcgaaa gtgatcgacg ttgatcttac aaatgaagag tatgcgttcg
     4621 gtattgataa agatcagccg gaacttgttg aacaggtaaa cgcatttata aaagaagtaa
     4681 aagaagacgg aacgctggat gagatctgtg acaagtattt cggcggcgga gagccggcag
     4741 cagtagaatc cgcaaaactt gacgagtcga aagatcagct tgtagttgcc acaaatgcag
     4801 cgtttgagcc gtttgagtat acaaaaggcg ataaatatgt cggaatcgac atggaactgg
     4861 cagctcttct tgcggaaaag cttggaaaag aacttgtaat ccagaatatg gattttgatg
     4921 cggtatgtct ttctgtagga cagcacaaat gtgatatcgc tatcgcagga cttacagtca
     4981 atgatgaaag aaaacaatat gtgacattct cagatacata ttatcaggcg tcacagcgtt
     5041 tgatcgttcc gagcgatgac gatacattcg acgatctgaa agatgcagat gcagttgcgg
     5101 caaaacttgg cgaactgaaa aaagaagata agatcggcgt acagcaggga acgacagcgc
     5161 aaagctacat cgaaggagac gaagctttcg gatttgaagg acttccggct acatgccagc
     5221 cgtataagag cggatcactt gcggttcagg atatgctgaa cggaaacatc aaatatgtga
     5281 tcatcgatgc agctccggct acagcgatca cagaagcgat taatgcaatg caataagttc
     5341 tcggaagcag aatgagcaat cggcgcaata cagaagagga tttcaaaaca gtatgaaatg
     5401 aaacggacga ttgagcggat gatttcggac tgagagtttt gcaaaaagaa tagaaaacag
     5461 tattaaatca gaaagggaaa tacaggtatg ttccgaaaac ggaaaatatc ggcgttttcc
     5521 tttctttgga cgtttgagag aagaaagcaa agaggaaagt aatgggtaat tttagtgcaa
     5581 aagtagataa gtttatagaa atatttatcg atcaaaacgg ctatgtaaaa gtggtgcaag
     5641 gtttgcagaa cacagtcctt attgcgattg tcggtctggt catcggtatt ttgatcggga
     5701 ctgttatcgc gacggcaagg gtgctgccga aatataaagt gctcccaaga gttttgaacg
     5761 gtatatgcag cgtttatgtg gcgatgttca gaggaacgcc gatggttgtg cagcttcttg
     5821 tattttatta tgtacttctt ccgatcatcg gatggaacat aacgggagtt caggtcgcga
     5881 tgcttgtgtt cggattgaac agcggcgcat atatttcgga aattatgcga agcggaatcc
     5941 aatcggtaga cgcaggacag atggaagcgg gacgtgcagt cggattaagt ttcggggcga
     6001 gtatgatgaa gattgtcatt ccgcaggctg tcaagaatat tcttccgact ctcggaaacg
     6061 aatttatcgc gcttatcaaa gaaacatcgg ttgtaagttt cgtaggcgcg gcggatctgt
     6121 atgtggcgtt taactatatc ggaacaaaca gttatgaatt catggttccg tatctcgtga
     6181 tggcgcttat ttatattgca ttgatccttg ttgtgacact gcttgtaaaa ttattggaaa
     6241 ggagcctgaa gaagagtgat agacgtaatt aatcttagaa aaagctttgg tgatcttgaa
     6301 atcttaaaag gcatcaatat cacgatcaat aaaggcgaca ttgtcgcggt gctcggaccg
     6361 tcaggttccg gaaaaagtac gtttctgcgc tgcttaaatt gtatggaaga tccgacatcc
     6421 ggaagcattg tttttaaagg agtcgatatc gcggatatga aagtcgatat caacgtgcat
     6481 cgccgccata tgggtatggt atttcaacat tttaatttgt tcaataataa aacagttctt
     6541 caaaacgtaa tgatggctcc ggcatatttg cagtgcaagg atctgaaaaa agcaaaaaat
     6601 aaaaacagaa tgatccggtt taaaaatata ttccgaagca aaaaagaaga acttatccca
     6661 attacaacga caaaagaaca gatcaaaagc gagacgaaaa agcaggctct tgaactgctc
     6721 ggaagaatcg gacttgaaga caaagcggac gtttacccgt ctacattgtc gggaggtcag
     6781 aagcaaagag ttgcgatcat tcgttctctt gcaatgaatc cggatgtgat cctgtttgac
     6841 gagccgacat cggcgcttga tccggagatg gtcggagaag ttcttgagtt gatgaagaag
     6901 ctggctcagg aaggaatgac gatggttgta gtcactcatg agatgggatt tgcgaaagag
     6961 gttgcgtcca gagtgatctt catggatgac gggcagattg ttgaggaagc tgaaccggaa
     7021 gaatttttcg gcaatccgaa gaatccgaga ctgaaagaat ttttgtcgaa gattttgtaa
     7081 aaagaatctg taagttgaaa aacgtgtaca tgtctgtgca cagaagaaca tatgaaaagc
     7141 agggtggatg agaaactctg ctttttgttt ttgaaagaat gaaaagtccg aagcagactt
     7201 tttgctttta tttaaagcga ggtggaaaat gagatacgat cgaatttata aggcgaagtt
     7261 tatagaacgt ccgaaccggt ttattgccta tgcagaatta aacgggaaaa aggaaacggt
     7321 tcatgtgaaa aatacaggaa gatgcgccga gcttcttcgg ccgggagcag atatttatat
     7381 tcaggaaagc gataatccgt ccagaaagac aaggtgggac ttgattgccg taaaaaaggg
     7441 cagtcgaatg atcaatatgg actctcagat tccgaataag gttgtaaaag aatggattga
     7501 acagggaaat ctttttggaa atgtgacttt gatccggcct gaaaccgtat acaaaaattc
     7561 tcgttttgac ctgtatgtgg aagcggaaga tcaaaacggg ggaatccgaa agatttttat
     7621 tgagatcaaa ggtgtgacat tagaagaaaa cggagtgtgc cgcttcccgg atgcgccaag
     7681 cgaacgagcc gtaaaacatc tggaagagct ttccgatgca aagaagaatg gatatgaggc
     7741 atatgtgttt tttgtgatcc agatgaaagg cgtgcgttat ttcacgccga atacagagac
     7801 acatcctcaa tttgccgaag cgcttcaaaa gacggcaaag gaaggtgtaa aagtgcttgc
     7861 ctatgactgt gatgtgacaa gcgatgcgat cgagctgagc gatgcggtag acgtcgttct
     7921 aggcaatccg atcttaaaag aatctgtccg gcctcttgta gagtggtttc gtgagaataa
     7981 gcgtgatctt ccgtggagaa agaggattaa cgcatacaga gtgtggattt cggagattat
     8041 gcttcagcag acgagggtgg aggcggtaaa gccttattat gagcgctttt taagtgaact
     8101 tcccgatgtg agcgcacttg catctgtcga ggaagacagg ttattgaaac tgtgggaagg
     8161 attggggtat tataaccggg cccgcaattt aaaagctgcg gcgtgtcaga ttatggaaca
     8221 atacggagga aggtttcctt catcatatga agaaatacgt tcgctgaagg gaatcggaaa
     8281 ttatactgcg ggagcgatcg gatcatttgt ttatcacatt ccgaaacccg cagtagacgg
     8341 aaatgttctg cgcgtagtat cgaggcttgc ggcagatgag ggtgatatca aaacggcggc
     8401 tgtaagatca aaagtagaag aattgatcga agaaatcatc ccgaaagatg cgccgggtga
     8461 ttttaatcag gggttgattg agttgggagc gatcgtctgt gttccgaacg gagagccgaa
     8521 atgcgccgcc tgtccgcttg aagcgctgtg caaagcacat aaggaaggac gggaaatgga
     8581 tttcccggtg aagaaaaagg cgaaagcaag aagaatagaa aagaggacgg tgtttatttt
     8641 tcgggataat gaaaaagtgg caatcaggaa acggcctcaa aaaggactcc ttgccggaat
     8701 gtatgaattt ccgaatgtgg aagggcacct caggacagag gaagtgatcg ggtatgcgca
     8761 gacggcaggg ctgactccgg tacgtgtaaa acggattgga acggcaaaac atattttcag
     8821 ccatgtagaa tggcatatga caggatatga actgctggtc gatgaactgg aaaaaacgtc
     8881 ggcgccaaac gtcggtcaaa tgtgtgtgga aaacggagcg gatggatcgg aaaatatatt
     8941 tgtaaagata aaacaattag aggaagagta tgcgatgccg tctgcatttg ataagtttgt
     9001 agagcacact cggttttctt gaaatgtatg agatactgtg gtaaactgtt gctgtatgta
     9061 ttgaatgaag tcagaacaga caggagtgtg cttggatgaa agaaaaagaa cagatctcaa
     9121 gacagcagca aaaatcccgg gagacgaaag aaaaaatatt caaggcagct aaaaccgtag
     9181 acagaattgg aacaagaatt gatttttt
//
LOCUS       DBMDXF010000065         9164 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2062_length_9164_cov_2.000883, whole genome shotgun sequence.
ACCESSION   DBMDXF010000065 DBMDXF010000000
VERSION     DBMDXF010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2062_length_9164_cov_2.000883"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            324..662
                     /locus_tag="ACZPD4_04025"
                     /pseudo
     CDS             324..662
                     /locus_tag="ACZPD4_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            862..934
                     /locus_tag="ACZPD4_04030"
     tRNA            862..934
                     /locus_tag="ACZPD4_04030"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:895..897,aa:Ala,seq:tgc)
     gene            1246..1767
                     /locus_tag="ACZPD4_04035"
     CDS             1246..1767
                     /locus_tag="ACZPD4_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1378275.1"
                     /translation="MRGQTEGSHEEINVEEAIRIHGNMVYRLALVQMKNKSEAEDVFQ
                     EVFLRLVKYKERIQGREHLKAWLLRVTVNCCKKQFDSAFRKKTVSIDRDVQSTDSYEM
                     DLPENLIYEAVLRLPADYRSTVHLFYYEQYSVSEIAEMLELSESAVKTRLYRSRGMLK
                     EALKGEFDCVGRI"
     gene            1751..2254
                     /locus_tag="ACZPD4_04040"
     CDS             1751..2254
                     /locus_tag="ACZPD4_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378276.1"
                     /translation="MWDEYKKVNDSIHVPEELVNRTVKAAEREERRRKIIGLWKYTAI
                     AACFCFVCLGIWGAAFKDKIVIQDVTFASSEMEIGLNLGKKDISETREWEDIQVEKYT
                     EKDDENIPKELWKLKPGRVHGEKVYIGKTEDGILHAVFEKDGKIWYVTEEKGDKENLT
                     EYLKKTL"
     gene            2342..4669
                     /locus_tag="ACZPD4_04045"
     CDS             2342..4669
                     /locus_tag="ACZPD4_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1378277.1"
                     /translation="MKKKYLKTCISVLAVTAVLLSACGGPQQKNVGKTTMETAKLADS
                     IRKKYNEKYEYADPIRGVARDERLKLKMGFDIMNAGFTEYTQIVNVYKDPELTYQIGS
                     HFEWDEKTKELSVTPPRWSVGGIMSEGLDEDAPGYQAADSSLFDKGELNDWGNLPQYY
                     MVQYVDLETGEKLEKPIVTVFTVDSEVKNAPRVTLGINEEGFPVFRWKKVPGAERYYV
                     MSMTSSEKMGLSGDGWVEGETEGTEWIPESTAKFITYNVSEADRREPYVIEKYGEGNE
                     AIPKDTEYETYYCVIAVSKDGTSALSNMFRETDIARKVPFTEEVKMSLNEEGSNYTQS
                     FGEMPSYKWVTMCDGTLVQKLINYDFESAKEEKASWSGENIVKVPYKIDGTGFDGVVV
                     VESYNTDTWKEELEKVRIRQEKLRNRAGAVTPEMKVEGETESEKEEKESSGESERKYE
                     VTANSALSEYLAVNMLNANTSISLKEFPESADTDYLLDAWEEAVYQNPMVLGVTGASI
                     ANGGKTLHVKYDTKSEEIKKKQKEISKEAERVVDSIITDDMTELEKEMAINQYLCDTA
                     EYDMDALENAEENDFAGVDAKFNDSFTPYGVLLNKTGVCSSYAGAFKLLAQQAGLECI
                     VVTGNLDGELPHAWNKIKIDGEWRIVDSTNNDNELILNALLNLPDYAADKVLVEDERF
                     VLDDKVKDYEAKSDDKEFYRIEGKFYEKNKIFEPLKNELKEEGIAVLRTDYTLSDKEF
                     MKIAQEVVEGYGSGELGGYYWLGVIYLTDEEGREK"
     gene            4742..6127
                     /locus_tag="ACZPD4_04050"
     CDS             4742..6127
                     /locus_tag="ACZPD4_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HIR1378278.1"
                     /translation="MKKRRNKLVTLGMAAMMTAALMSGCGKKATPENLLKDMAKNMKD
                     TESVVANMKMDMEMGDDTTTIGIQMDMDIDMDLKSGESHAKGAVDMKMMGTNVGTEIE
                     MYTVKEKDQYIVYTMANDQWQKEEMDDSAADMAGELSDLTGSMGGHAKSFEMSKEKVE
                     VNGEKCFEMKGEIKGSDIVGMMESGMADSLGSEIDEDVIKDLELPCTIAVYEDDILPA
                     KISFDMQDIMEKAMEEEGVDVSACSIEVTYHEYDSVGEIKVPNDVIEKAGGEVSDDKE
                     DKEDKDDDKKADKPAEQSKELGAKWDSYTVQINDKVLKLPCSLADLEATGLKLDREYT
                     PEDYVVNAGEYELAWFEDENGNTIMADMVNTGDDPKALKDCIVGAISVDDYDLEEGGL
                     TVLFPGGITVGTKEADVLAAYGEPTDSYKDEEYGNTYYWYDKESYDNSCTVETDAETG
                     LVENITLQCYE"
     gene            6372..8645
                     /locus_tag="ACZPD4_04055"
     CDS             6372..8645
                     /locus_tag="ACZPD4_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1378279.1"
                     /translation="MIKLYENGVYLLNGTEIVEDTGSAQVSVSKEEAAKNTIAYGVLE
                     AHNTSSDMKNLQIKFDKLTSHDITFVGIIQTARASGLEKFPIPYVLTNCHNSLCAVGG
                     TINEDDHMFGLTCAKKYGGMYVPPHQAVIHQFAREMLAGGGKMILGSDSHTRYGALGT
                     MAMGEGGPELVKQLLNKTYDIKMPGVIAVYLEGEPMKGVGPQDVALAIIGATFKNGYV
                     NNKVMEFVGPGVKNLSADFRIGIDVMTTETTCLSSIWTTDEKIEEFYEIHGRKEEYKE
                     LNPGAVTYYDGCVRVNLSEIKPMIAMPFHPSNVYTIDELNANLSDILHDVEQKALVSL
                     DGAVEYSLQDKIRDGKLYVDQGIIAGCAGGGFENICAAADIIKGHNIGSDAFTFSVYP
                     ASTPIYMELVKNGVVADLMEAGTVVKTAFCGPCFGAGDTPANNAFSIRHTTRNFPNRE
                     GSKLQSGQISSVALMDARSIAATAANKGYLTPATAFDVEYKGQKYHFDQNIYANRVFD
                     SKGKADPNVEIQFGPNIKDWPEMSALPENLIVKVVSEIHDPVTTTDELIPSGETSSYR
                     SNPLGLAEFALSRKDPAYVGRAKEVQKAQKAIEAGTCPLDALEELKPVMSKIQAAYPE
                     VGEGNVGVGSTIFAVKPGDGSAREQAASCQKVLGGWANIAVEYATKRYRSNLINWGML
                     PFLTDADHEALPFKNGDYIFVPNVRKAVEEKKDVINAYVVGDELKEIQLKLGDLTDAE
                     RQIILDGCLINYYKATK"
     gene            9024..>9164
                     /locus_tag="ACZPD4_04060"
     CDS             9024..>9164
                     /locus_tag="ACZPD4_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378280.1"
                     /translation="MLRELRKQKKLTQAECAKYLGVPLRTYQNYETDAAKRTSIKYLY
                     MLE"
BASE COUNT         3108 a         1404 c         2376 g         2276 t
ORIGIN      
        1 taaaatatat cattgtctct gaagaagaga tatgttatga taaatccact cacagaaatt
       61 ctttttgtga aagatgccga cagcttcgtc ggtcagagga attccctcaa aattacccag
      121 aacacaacga aacagatttg catggatagt cttgtagaac gtctcttttt ttcgtatact
      181 gattcttaac agaagaaaaa agtttcaccg aaagttataa gagaaagctt taggagaaaa
      241 agctttgaga gaaaaaactt aagagtgttt tcttacttgt acgataggac tcattcatgc
      301 acatgaaagg agagcctatg gtaggaaaag aaaagtgggc aaaagaaaaa gaactgcgct
      361 tgtcagatgc ttatattgta aaagataatg aaccaagttt ggaattaaaa gtaaaggtta
      421 ttaatatccg accggaagaa catcatgaga ttttagagaa atgtcaagtg ctgaaagagt
      481 atagccagtt tatggaaatc gtgcagaact atcagatctc cggtgtggaa gaaccttata
      541 aaaaggcgat aaaagagtgc attgaaaaag gaaaaacaat ttccgaaatt gcagatgagc
      601 ttgaagatac agaagaaaat attgctcgtc tgatagaaga gttttatctt cgtataaatt
      661 gatcatagtt aaatctataa aatgggggat aagttttgat ttctcccctg tctggaaggt
      721 gtatttatat attttttaca acgaatacga aaagtgggaa atggaagctg catgttttgg
      781 catgaatttg tcgaaaattt cataaaaaac ttgacaagtt tcttagaata tgatatctta
      841 taaaagttct aagaacgcta tgggggatta gctcagttgg gagagcgcct gccttgcaag
      901 caggaggtca cgagttcgac tctcgtattc tccactttaa ataaagatcc gaacattggc
      961 attagtcagt gttcggattt tttatgctat aggaaattac tccgtaatgt tccatagcat
     1021 aaaaaacgct ccgtgtagga gcgcactgcg gcggaaaaga agtacgtcgc taaagcgacc
     1081 cgccgcaggc ggagaatctt gcgagcagga ttctttttta tattgtgcaa cgagccaaag
     1141 tccgggtttg ttactttgtt cttgtatgaa gaaaaaaagt aagatataat caaaaaataa
     1201 acataaaatc tatgtgacgt ttaagtacgg gaaaaggtat tatatatgag aggtcaaaca
     1261 gagggcagtc atgaagaaat taatgtagaa gaagccatcc gtattcatgg aaatatggtt
     1321 tatcggcttg ctctggtaca aatgaaaaat aaaagtgaag cagaagatgt gtttcaggaa
     1381 gtgttcctcc gattagtaaa gtataaagaa agaattcagg ggagagagca tttgaaggca
     1441 tggcttttga gagtaactgt taattgctgc aagaaacagt ttgatagtgc gtttcggaaa
     1501 aagacagttt ctatagacag ggatgttcag agcacggaca gttatgagat ggatctgccg
     1561 gagaatctca tatatgaggc agttcttcgc ctgccggcag attacaggag tacagtacat
     1621 ttgttttact atgaacaata ttctgtttct gagattgctg agatgttgga actttcggag
     1681 tcggctgtaa aaacccgcct gtatcgcagc agggggatgc ttaaggaagc tttgaaagga
     1741 gagtttgact gtgtgggacg aatataagaa agtaaatgac agcattcatg tcccggaaga
     1801 attagtgaac cggacggtaa aagcagctga aagagaggag cgccgccgca aaattatcgg
     1861 attgtggaaa tatacggcaa tagcggcatg cttttgtttt gtctgccttg gtatatgggg
     1921 agcggcgttt aaagacaaga ttgtgataca agatgtgaca tttgcttcat cagagatgga
     1981 gattggatta aatctcggaa aaaaggacat atcagagacg agagagtggg aagatattca
     2041 agttgaaaag tatacggaga aggatgacga gaatatcccg aaagagctgt ggaagctgaa
     2101 gccgggaagg gttcacggag aaaaagtata tataggaaaa acggaagacg gaattcttca
     2161 tgcagtgttt gaaaaagacg ggaaaatctg gtatgtgaca gaagaaaaag gggataaaga
     2221 aaatctgaca gaatatttga aaaaaacttt ataaaagacg taaccttttg gatgcggcaa
     2281 aggtattata agcagaaacg cataaaaaaa ctgtttgttg ttcatcaaag aggaaatgct
     2341 tatgaaaaag aaatatttga aaacatgtat cagtgttttg gcagtaacgg cagtcttgct
     2401 aagcgcatgc ggaggtccgc agcagaaaaa cgtcgggaag acaacgatgg agactgcaaa
     2461 actggcggat tctatcagaa aaaaatataa tgaaaagtat gaatatgccg atccgatccg
     2521 cggcgtggct cgtgacgaaa ggcttaagct gaaaatgggg tttgatataa tgaatgccgg
     2581 atttacggaa tatacacaga tcgtaaatgt gtataaagat ccggaactca cctatcagat
     2641 cggatcacat tttgagtggg atgaaaaaac aaaggaatta tcagttacac ctccgagatg
     2701 gagtgtaggc gggatcatga gcgaaggtct tgacgaggat gcgccgggat atcaagcggc
     2761 agattcgtct ttgtttgata aaggagagtt gaatgactgg ggtaatcttc cacagtatta
     2821 tatggttcaa tatgtggatc ttgaaaccgg agaaaaactg gagaaaccga tagtgacagt
     2881 gtttaccgtc gattcagaag ttaagaatgc accgagagtt actcttggga ttaatgagga
     2941 aggatttccg gttttcagat ggaaaaaagt gccgggagca gagcgttatt atgtgatgag
     3001 catgacgtcc tcagaaaaga tgggattatc cggagacggc tgggtcgaag gtgaaacaga
     3061 aggaacagaa tggattcccg aatcaacggc aaagtttatt acttataatg tgtcggaagc
     3121 ggatcgccgg gagccatacg ttattgaaaa gtacggagag ggaaacgaag cgataccgaa
     3181 agatacagag tatgaaactt attactgcgt tatagcagta tcaaaggacg gaacatctgc
     3241 tttaagcaat atgttcaggg aaacagatat tgcgcgtaaa gttcctttta cggaagaagt
     3301 gaagatgagc ctgaatgagg aaggttcaaa ttatacgcag tcgttcggcg aaatgccgtc
     3361 atataaatgg gtgacgatgt gcgacggaac gcttgtacaa aagctgatta attatgattt
     3421 tgaaagcgcg aaggaagaga aggcttcatg gagcggtgaa aatatcgtaa aagttccgta
     3481 taagattgac ggaacaggat ttgacggagt tgttgtcgta gaaagttata atactgatac
     3541 ttggaaagaa gaactggaaa aggttcgaat cagacaagag aaattaagaa atcgtgcagg
     3601 tgcggttact ccggagatga aagtggaagg cgagaccgaa agtgaaaaag aggaaaagga
     3661 gtcttcggga gagagtgaac gaaagtatga agtcactgca aatagcgctt tgagtgaata
     3721 tctggcagta aatatgctca atgcgaacac aagtatcagt ttgaaggaat ttccggaaag
     3781 tgcagatacg gattatctgt tggatgcatg ggaagaagcg gtttatcaaa acccaatggt
     3841 actcggagtg acgggggcgt caatcgcaaa cggcggaaag acactgcatg tcaaatatga
     3901 tacaaaatcg gaagaaataa agaaaaaaca aaaagaaatc tcgaaagagg cagaacgtgt
     3961 tgtagacagt attattacgg acgatatgac agaacttgag aaagaaatgg cgatcaatca
     4021 atatctgtgc gatactgctg aatacgatat ggacgcgctt gaaaatgcag aagaaaacga
     4081 ttttgccgga gtcgatgcaa agtttaatga ttcctttaca ccgtatggcg ttttgttaaa
     4141 taaaacagga gtgtgttcaa gttatgccgg agcattcaaa cttctggcgc agcaggccgg
     4201 actggaatgt atcgttgtga caggaaatct ggacggtgaa cttccccacg catggaataa
     4261 gattaaaata gacggggagt ggaggatcgt agattccacg aataacgata atgagttgat
     4321 cttaaatgcg ctgttaaatc ttcccgatta cgcggcagac aaagtgcttg tagaagacga
     4381 acgttttgta cttgacgata aggtgaaaga ttatgaagca aagtcggatg acaaagagtt
     4441 ttaccggata gaaggaaaat tttatgagaa aaataaaatt tttgagccgc ttaaaaatga
     4501 attaaaagaa gagggaattg cggtattgcg tacagattat acattaagcg ataaagagtt
     4561 tatgaagatc gcacaagaag tagtcgaagg atacggaagc ggagaactgg gaggatatta
     4621 ctggcttggg gtaatttatt taacagacga agaaggaaga gaaaaataaa agaaaacaga
     4681 gcgagagcag aaaatataaa agtatataat ataaatttat atataacgag gaggaaaagg
     4741 tatgaaaaaa cgtaggaaca aattagtgac actgggaatg gcagcgatga tgacagcggc
     4801 gcttatgtca ggatgcggga aaaaggcgac tccggaaaac ttattaaaag acatggcgaa
     4861 aaacatgaaa gacacagagt cggtagtggc aaacatgaag atggatatgg agatgggaga
     4921 cgatacaact acgatcggca tccagatgga tatggatatc gacatggatc ttaaatcagg
     4981 agaaagtcac gccaaaggcg cggtagacat gaaaatgatg ggcaccaatg tcggaacaga
     5041 gattgaaatg tatactgtaa aagaaaaaga tcagtatatt gtgtatacga tggcgaacga
     5101 tcagtggcaa aaagaagaga tggatgattc tgcggcagat atggcgggag aactttcgga
     5161 tcttacagga agtatgggag gacacgcaaa atcttttgag atgtccaaag aaaaggtgga
     5221 agtaaacggc gaaaaatgtt ttgagatgaa aggcgagatt aaaggctctg acattgtggg
     5281 catgatggaa tcaggtatgg cagacagctt aggttctgaa attgacgaag atgtgatcaa
     5341 agatcttgaa cttccgtgta cgatcgccgt ttatgaagat gatattcttc cggcaaagat
     5401 ttctttcgat atgcaggata ttatggaaaa ggcgatggaa gaagaaggcg ttgatgtttc
     5461 ggcatgctcg atcgaagtga cataccatga atatgacagt gtaggcgaga ttaaagttcc
     5521 gaacgatgtg attgaaaaag caggcggaga agtgtctgac gataaagagg acaaagaaga
     5581 taaagacgac gataaaaaag cggataaacc ggcagagcag agtaaagaac ttggcgcgaa
     5641 atgggacagc tatacggttc agatcaacga taaagttctt aagcttccgt gcagtctggc
     5701 agatctggaa gccacaggtc tgaaactcga cagagaatat acacctgaag actatgttgt
     5761 aaatgcaggc gaatatgagt tggcatggtt tgaagacgaa aacggaaata cgattatggc
     5821 ggatatggta aacacaggag atgatccgaa agcattgaaa gattgtatcg taggagcgat
     5881 ttctgtagat gattatgatc ttgaagaagg cggactgaca gttttgttcc cgggcggaat
     5941 tacggttgga acgaaagaag cggacgttct tgcagcttac ggcgaaccga cggattcgta
     6001 taaagatgaa gaatatggaa atacttatta ctggtatgac aaagaaagtt acgacaactc
     6061 atgtacagtt gaaacagatg cagaaacggg tcttgtcgaa aatattaccc tgcagtgtta
     6121 tgaataaaga tttcgagaga taaagcttga aaaaatatat catagcctga aaaagggcgg
     6181 gaggtctttc cggaaattcg tttgcggaag atattcccgc ccttttcgtt gtatggctga
     6241 acgattccgg aagaagttgt ttttttactg tatgcagaag ttgttttttt gctgtattgg
     6301 ttacattttc ataataagta tgataatata tagaaatagc gtaaaattga caaacgaaaa
     6361 ggaggtttca tatgattaaa ttatatgaaa acggtgtgta tttgctcaat ggcactgaga
     6421 ttgttgaaga tacagggagc gcacaggttt cggtttctaa agaagaagca gcgaaaaaca
     6481 cgatagctta cggcgtgtta gaggcgcata atacttcttc tgatatgaag aacctgcaga
     6541 ttaaatttga taaattgaca tcacatgata ttacttttgt cggaattatt cagacagccc
     6601 gtgcttcggg tcttgagaag tttccgattc catatgtatt gacaaactgt cataacagcc
     6661 tttgtgcggt tggcggtacg attaatgaag acgatcatat gtttggtctg acatgtgcga
     6721 aaaaatacgg cggcatgtat gttccgccgc atcaggcagt tatccatcag tttgcgagag
     6781 agatgctcgc aggcggcgga aagatgatcc tcggatcgga cagtcataca cgttatggcg
     6841 cacttggtac aatggctatg ggagaaggcg gtcctgaact tgtaaaacag ctcctgaaca
     6901 aaacgtatga tattaagatg ccgggagtga tcgctgttta tcttgaaggc gagccgatga
     6961 aaggggtagg tccgcaggac gttgcgcttg caattatcgg ggctacattt aaaaacggct
     7021 atgttaataa taaagtaatg gaattcgtcg gaccgggcgt gaaaaactta agcgctgatt
     7081 tccgtatcgg aatagatgtt atgacgacgg agacaacctg tctttcatcc atctggacga
     7141 cagatgaaaa gatcgaagaa ttctatgaga ttcacggaag aaaagaagaa tataaagaat
     7201 taaatccggg agcggttaca tattatgacg gatgtgttcg tgtgaactta agtgagatca
     7261 aaccgatgat cgcaatgccg ttccatccaa gcaacgtcta tacgattgat gaattgaacg
     7321 caaatctgtc agacatcctt catgacgtag agcagaaagc tctcgtaagt cttgacggag
     7381 ctgtagagta ttctcttcag gacaagatcc gagacggaaa actctatgta gatcagggaa
     7441 ttatcgcagg atgtgcggga ggcggttttg aaaatatctg tgcggcagca gatatcatca
     7501 aaggtcataa tatcggatcg gatgcattta cgttcagcgt atatccggca agtacgccga
     7561 tttatatgga acttgtgaaa aatggtgtcg tagccgatct tatggaagcg ggaacagttg
     7621 taaaaacagc attttgcggt ccgtgtttcg gagcgggaga tacaccggca aataatgcgt
     7681 tctctatccg tcatacaacg agaaacttcc cgaaccgtga aggaagcaag cttcagagcg
     7741 gtcagatttc ttctgttgct cttatggatg ctcgttccat tgcggcgaca gcggcaaata
     7801 aaggatatct gacgcctgct acggcatttg atgtagaata taaaggacag aagtatcatt
     7861 ttgatcagaa tatttatgcg aatcgtgtgt tcgacagcaa aggcaaagca gatccgaatg
     7921 tagagatcca gtttggtccg aatattaaag actggccgga gatgtcggcg cttcctgaga
     7981 atctgatcgt gaaggtcgta tctgaaatcc acgatccggt aacgacaaca gatgaactta
     8041 ttccgtcagg agagacgtct tcgtatcgtt ctaatccgct tggacttgca gagtttgcac
     8101 tttcaagaaa agatcccgca tatgtgggaa gagcaaagga agttcagaaa gcacagaaag
     8161 caatcgaggc aggaacatgt cctcttgatg ctctggagga actgaaaccg gttatgtcta
     8221 agatccaggc agcgtatccg gaagtcggag aaggaaatgt cggtgtagga agtacgatct
     8281 tcgcagtaaa accgggagat ggatcggctc gtgagcaggc tgcctcctgc cagaaagttc
     8341 tcggaggctg ggcaaacatt gctgtagaat atgcgacaaa acgttatcgc tccaacctga
     8401 ttaactgggg aatgcttcca tttttgacag atgcggatca tgaggcgctt ccgtttaaaa
     8461 acggagatta cattttcgtt ccgaatgtga gaaaagcagt tgaggaaaag aaagacgtta
     8521 tcaacgcata tgttgttggt gatgaattga aagagatcca gctgaaactc ggggatctga
     8581 cagatgccga gagacagatt attctggatg gatgtctgat caactactat aaggcaacaa
     8641 aataaaacag taaaataata gaatcccctg acagcgagat gcaaaagaaa catctgcggc
     8701 ggtcagggga ttttttgtaa cggaaaattc atttctgtgt gaaagatgtt ctgagtgtgg
     8761 gggttagtat gctgaaagta ttagagtgga tgtgcagttt gtggggatga aaatgttatg
     8821 tagatgataa taagaggtga ttacgggtat aataaatgtg caagaattta tggaaatata
     8881 aaagaaatag aaattgcgtt ttttgataag taataatacg caaaacatgc gtgcttaaac
     8941 tgcaaaaaat acgcgaaatt tgcgcgtttt aattgcgcgt ttccatattt tatggtagca
     9001 tttgaatgag gtggaaaaat atgatgctga gagaactgcg gaaacagaaa aaactaaccc
     9061 aagcggaatg tgcgaaatat ttaggagtgc cattgcgtac ttatcaaaat tatgaaactg
     9121 atgcagctaa gcgtacgtca ataaaatatt tgtatatgct cgaa
//
LOCUS       DBMDXF010000066         9104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2077_length_9104_cov_2.100078, whole genome shotgun sequence.
ACCESSION   DBMDXF010000066 DBMDXF010000000
VERSION     DBMDXF010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_9104_cov_2.100078"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..2718)
                     /gene="adhE"
                     /locus_tag="ACZPD4_04065"
                     /gene_synonym="adhC"
     CDS             complement(100..2718)
                     /gene="adhE"
                     /locus_tag="ACZPD4_04065"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIR1378281.1"
                     /translation="MAKKEKTVVPEIVDSVEALEAKMKAMREAQKVFATFTQEQVDKI
                     FYEAAMAANKQRIPLAKMAVEETQRGIVEDKVIKNHYAAEYIYNAYKNTKTCGVIEED
                     TAYGIKKVAEPIGLVAAVIPTTNPTSTAIFKTLICLKTRNAIIISPHPAAKECTIAAA
                     KVVLEAAVKAGAPEGIIGWIDAPSLELTNTVMRDADIILATGGPGMVKAAYSSGKPAL
                     GVGAGNTPVIIDDTADVRMAVNSIIHSKTFDNGMICASEQSVTVLESVYEQAKKEFAY
                     RGCYFLKPGKELDAVRKTIIINGALNSKIPGKSAYEIAKLAGVNVPEDTKILIGEVES
                     VDIAEEFAHEKLSPVLAMYKAKTFDEALDKAAQLVADGGYGHTSSLYVHPAEKEKIQK
                     HYEAMKTCRVLINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVGVKHLLNVK
                     TVAERRENMLWFRTPEKVYFKKGCMPVALDELGTVMHKKKAFIVTDTFLYKNGYVDPI
                     EKKLDEMGIQHTCFYNVAPDPTLQCAQEGVAQMRAFEPDTIIALGGGSAMDAAKIMWL
                     MYEHPEANFEDMAMDFMDIRKRVFTFPKMGEKAYFVAIPTSSGTGSEVTPFAIITDAE
                     TGVKWPIADYELLPNMAIVDVDNAMTAPKGLTSASGIDVMTHAIEAYVSIMATDYTDG
                     LAMKAVKMVFENLPSAYENGANDPKAREEMANASCMAGMAFANAFLGVNHSMAHKLGA
                     FHHLPHGVANAVILTEVMRYNAAEVPTKMGTFSQYQYPHALARYAELGRFAGCTGNTD
                     EEVFENFIAKLEDLKEKIGIKKTIKDYGIDEKYFLDTLDDMCEQAFNDQCTGANPRYP
                     LMKEIKEIYLKCYYGK"
     gene            complement(2998..3516)
                     /locus_tag="ACZPD4_04070"
     CDS             complement(2998..3516)
                     /locus_tag="ACZPD4_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR1378282.1"
                     /translation="MAKYRKLGRTSSQRKALIRNQVTALLNNGKIVTTEAKAKEIRKT
                     AEGLIALAVKEKDNFDEVTVMAKVARKDKDGKRVKEVVDGKKVTVYDEVEKKIKKDQP
                     SRLHARREMLKVLYPVKEGENKKAKEVDMVAKLFDEIAPKYADRNGGYTRIVKIGQRK
                     GDAAMEVLLELV"
     gene            complement(3778..>4670)
                     /locus_tag="ACZPD4_04075"
     CDS             complement(3778..>4670)
                     /locus_tag="ACZPD4_04075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1378283.1"
                     /translation="VEPLERGYGTTLGNSLRRIMLSSLPGSAISSVKIDGVLHEFSSI
                     PGVKEDVTEIIMNLKSLAIRNMSESDEPKTVYIEFEGEGVVTGADININDPDIEIVNP
                     DQVIATLSGGKDCRLNMELTITKGRGYVSSDKNKTEDMPIGVIAIDSIYTPVERVNLT
                     VENTRVGQITDYDKLTLDVWTKGTMLPDEAVSLAAKVLSEHLKLFVDLSEVAQVAEVM
                     IEKEDDEKEKVLEMSIDELELSVRSYNCLKRAGINTVEELTNKTPEDMMKVRNLGRKS
                     LEEVLAKLKELNLELNQGEE"
     assembly_gap    4671..4680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4802..5395)
                     /gene="rpsD"
                     /locus_tag="ACZPD4_04080"
     CDS             complement(4802..5395)
                     /gene="rpsD"
                     /locus_tag="ACZPD4_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR1378284.1"
                     /translation="MAVNRVPVLKRCRSLGMDPIYLGINKKSNRQLKRANRKMSEYGL
                     QLREKQKAKFIYGVLEKPFRNYYAKAKQMEGMTGENLMRILESRLDNVIFRMGLARTR
                     KEARQIVDHKHVLVNGKQVNIPSYLVKAGDTIEIKENKKGSQRYKDILEATGSNMVPE
                     WLDVDAENLKGSVKELPAREAIDVPVDEMLIVELYSK"
     gene            complement(5481..5876)
                     /gene="rpsK"
                     /locus_tag="ACZPD4_04085"
     CDS             complement(5481..5876)
                     /gene="rpsK"
                     /locus_tag="ACZPD4_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1378285.1"
                     /translation="MAKKVAKKVTKKRVKKNVERGQAHIQSSFNNTIVTLTDTQGNAL
                     SWASAGGLGFRGSRKSTPYAAQMAAETAAKAALVHGLKSVDVFVKGPGSGREAAIRAL
                     QACGIDVVSIKDVTPVPHNGCRPPKRRRV"
     gene            complement(6028..6396)
                     /gene="rpsM"
                     /locus_tag="ACZPD4_04090"
     CDS             complement(6028..6396)
                     /gene="rpsM"
                     /locus_tag="ACZPD4_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR1378286.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIYGIGRTSASRILADAGVDPNTR
                     VRDLTSEEVKKISAVIDETQVVEGDLRREIQLNIKRLKEIGCYRGIRHRKGLPVRGQK
                     TKTNARTCKGPKRTVANKKK"
     gene            complement(6788..6901)
                     /gene="rpmJ"
                     /locus_tag="ACZPD4_04095"
     CDS             complement(6788..6901)
                     /gene="rpmJ"
                     /locus_tag="ACZPD4_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR1378287.1"
                     /translation="MKVRSSVKPICEKCKIIKRKGSIRVICENPKHKQRQG"
     gene            complement(7188..7406)
                     /gene="infA"
                     /locus_tag="ACZPD4_04100"
     CDS             complement(7188..7406)
                     /gene="infA"
                     /locus_tag="ACZPD4_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR1378288.1"
                     /translation="MSKADVIEIEGVVVEKLPNAMFKVELENGHIVLAHISGKLRMNF
                     IKILPGDKVTLEMSPYDLSKGRIIWRDK"
     gene            complement(7454..8098)
                     /locus_tag="ACZPD4_04105"
     CDS             complement(7454..8098)
                     /locus_tag="ACZPD4_04105"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR1378289.1"
                     /translation="MKIIMLGAPGAGKGTQAKKIAEKYSIPHISTGDIFRANIKNGTE
                     LGKKAKTYMDQGLLVPDELVVDLVVDRVNQDDCANGYVLDGFPRTIPQAEALDKALAD
                     MGQKIDYAIDVEVPDENIVNRMSGRRACVDCGATYHLVYAPTKEEGICDKCGGGLILR
                     DDDKPETVLKRLGVYHDQTQPLIDYYTQSGILKEVDGTIDIDDVFAEIVRILGE"
     gene            complement(8255..>9104)
                     /locus_tag="ACZPD4_04110"
     CDS             complement(8255..>9104)
                     /locus_tag="ACZPD4_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR1378290.1"
                     /translation="ITENGVGNGISIVLLINIISRVPSDLYTLYEKFMSGKKLAQAGL
                     AGLIILAVILAVVVFVVLLQSGERRIAVQYSKKVQGRKTYGGQSTHIPLRVNTAGVIP
                     VIFASSLMQTPIVIAQFLGKGNGTGIGSQILAAMNSNNWCNPAKPVYTVGLLVYILLT
                     IFFAYFYTSITFNPLEIANNMKKSGGFVPGIRPGKPTVEYLQKILNYIIFIGACGLII
                     VQVIPFFFNGMFGANVSFGGTSLIIIVGVVLETIKKIESQMLVRNYTGFLNNKGSNAS
                     NSFLGY"
BASE COUNT         2211 a         2221 c         1602 g         3060 t
ORIGIN      
        1 ttttgtacat agatttatat gtttgaaccg aaataatttg aaaaaagcaa acagagcgct
       61 gtgaaagcag cgctctgtta ttattcgcag ttacggttct tatttaccgt agtagcattt
      121 caggtagatt tctttgatct ccttcataag cggatatctc gggtttgctc ctgtacactg
      181 gtcgttaaat gcctgttcgc acatatcatc caaagtatca aggaagtatt tctcgtcaat
      241 accgtaatct ttaattgttt tcttaatacc gatcttttct ttcagatctt caagttttgc
      301 gatgaagttc tcaaatactt cttcgtctgt gtttcctgtg catcctgcaa aacgtccaag
      361 ctctgcatat cttgcaagtg catgcggata ctgatactgt gagaatgttc ccatctttgt
      421 cggaacttct gccgcattat atctcataac ttccgtaagg attacagcgt ttgcaacacc
      481 gtgcggtaag tgatggaatg caccaagttt atgagccata gagtggttca caccaaggaa
      541 cgcatttgca aatgccatac ctgccataca ggaagcgttt gccatctctt cacgagcttt
      601 cggatcgttt gctccgttct cgtatgctga cggaaggttt tcaaatacca ttttaacagc
      661 tttcattgca agaccgtctg tatagtctgt tgccatgata gatacatatg cttcgatcgc
      721 atgtgtcata acgtcgatac ctgatgcgct tgtcagtccc ttcggagctg tcattgcatt
      781 gtcaacgtca acgattgcca tattcggaag aagttcgtag tcagcgatcg gccatttaac
      841 gcctgtttca gcatctgtaa tgatcgcgaa cggtgtaacc tcagaacctg ttcctgaaga
      901 tgtcggaata gctacgaagt atgctttctc tcccattttc gggaatgtaa atactctctt
      961 acggatatcc atgaagtcca tagccatgtc ttcaaagttt gcttccggat gctcatacat
     1021 aagccacatg atcttagcag catccattgc agatccaccg ccgagtgcga taattgtatc
     1081 cggctcgaat gctctcatct gagcaacacc ttcctgagca cactgcagag tcgggtccgg
     1141 agctacgtta tagaagcatg tatgctgaat tcccatctca tccagtttct tctcgatcgg
     1201 atctacatat ccgtttttgt aaaggaatgt atctgtaacg atgaatgctt tctttttgtg
     1261 cattactgtt cccagctcgt caagcgctac cggcatacat cctttcttaa agtaaacttt
     1321 ctcaggtgtt ctaaaccaca gcatattctc tctcctctcg gcaactgttt tcacgttcag
     1381 aagatgtttt acaccaacgt tttctgatac ggagtttcca ccccatgatc cacatccaag
     1441 tgtaagtgac ggagcaagtt taaagttgta aagatctccg ataccaccct gtgaagccgg
     1501 tgtattgatc agaacacggc aagttttcat tgcttcgtaa tgtttctgga tcttctcttt
     1561 ctctgccgga tgtacataca gagaagatgt atgtccgtat ccgccgtcag ctacaagctg
     1621 tgcagcttta tcaagagctt cgtcaaatgt ttttgcttta tacattgcaa gtaccggtga
     1681 cagtttctcg tgagcaaatt cttctgcgat atctacagac tcaacttcac cgataaggat
     1741 ttttgtatct tccggaacat ttacacctgc aagttttgcg atttcatatg cagattttcc
     1801 cggaattttg ctgttaagag ctccgttaat gatgattgtt ttacgaaccg catcaagctc
     1861 ttttccaggt ttcaggaagt agcagcctct gtaagcgaac tcttttttcg cctgctcgta
     1921 tacgctctcc agaacagtta cagactgctc ggaagcacag atcataccat tatcaaatgt
     1981 tttagaatgg ataatagagt ttacagccat tcttacatct gctgtgtcat caatgataac
     2041 cggtgtattt ccggcgccaa cgccaagagc cggttttcct gaagagtaag cagcttttac
     2101 cattcccggg ccacctgttg ccagaatgat gtctgcgtct ctcataactg tgtttgtgag
     2161 ctccagtgac ggtgcatcga tccatccgat gattccttcc ggcgctcctg cttttacagc
     2221 agcttcaagt acaacttttg cagctgcgat cgtacattct tttgcagccg ggtgtgggct
     2281 gatgatgatc gcatttcttg tcttcaaaca gatcaatgtt ttgaagattg ccgtagaagt
     2341 cgggtttgtt gtcgggataa ccgcagcaac aagaccgatc ggctctgcta cttttttgat
     2401 tccgtaagct gtatcttctt cgatcacgcc acaagttttt gtgttcttgt atgcgttata
     2461 tatatactct gcagcatagt ggtttttgat aactttatct tcaacaatac ctctctgcgt
     2521 ctcttctact gccatctttg caagcgggat acgctgtttg ttggctgcca tagctgcttc
     2581 atagaaaatc ttgtcaacct gctcctgagt aaatgtcgcg aaaactttct gtgcttcgcg
     2641 cattgctttc atttttgctt caagcgcttc tacactgtca acaatctcag gtacgactgt
     2701 tttttctttc tttgccattt gataattcct cctgtaattg agttccggtt tatcttttca
     2761 accgttatct atcttatttc tcacaagttc aagtatatct gtttgttaaa taattgtcaa
     2821 caacatattt gttaatattt taacggtatt ttttttgtat tatttgtgca ttttctacaa
     2881 aacagtaaaa atcctatgaa aattatcatt gtaaaaaaac tcctcctatg aatttcatag
     2941 aaagagtttc tcattgattc aatattaatc tgccgaaaat attcgggaac tcagctatta
     3001 tacaagctca agaagaactt ccatcgccgc gtcaccttta cgctgaccga tcttcacgat
     3061 tcttgtgtaa ccaccgttac gatctgcata tttcggagcg atctcatcaa ataattttgc
     3121 aaccatatcg acttcttttg cctttttgtt ctcgccctct ttaaccgggt aaagaacttt
     3181 cagcatctca cgtcttgcgt gaagtctgct cggctgatct tttttgatct ttttctctac
     3241 ttcatcgtaa acagtaactt tctttccgtc tacaacttct tttactctct tgccgtcttt
     3301 atctttgcgg gcaacttttg ccattactgt tacttcatcg aaattatctt tctctttcac
     3361 agccaacgcg ataagacctt ctgctgtttt acggatttct tttgctttcg cctcagttgt
     3421 aacgatcttt ccgttgttaa gcagagctgt tacctgattt ctgatcaatg cttttctctg
     3481 gctggatgtt ctgccgagtt ttctatattt tgccattctt atatttcctc ctaatctttc
     3541 cgtcataatt cggatgcgcc gtctgcagaa ccgcgcttcg cacgtctgcc gctattgttt
     3601 ataaacaaaa ccttaacgca tactcgttct ttttgaaaaa cacgccggca cgcattttcc
     3661 ctcccggtat atgtctgaac tatgactaca cattcaatta tgcatcttcg ggcttattaa
     3721 tcaaatgctc gcggatcgtc ctgtattccg aacaatccac gattttctat aaagcgatta
     3781 ttcctcgccc tgattcaact caagattgag ttcttttaac ttagcaagca cttcctcaag
     3841 tgatttacgt ccgaggttac gaactttcat catatcttca ggagtcttat tagtaagttc
     3901 ttctaccgta ttgattccgg ctctcttcag acaattatag gaacgaacgg aaagttcaag
     3961 ttcatcaatg ctcatctcaa gaactttctc tttctcatcg tcttctttct cgatcattac
     4021 ttccgcaacc tgagcaactt ccgaaagatc aacgaaaagt ttcaaatgtt cgctcagcac
     4081 ttttgccgca aggcttaccg cctcatcagg aagcattgtt ccttttgtcc aaacatcaag
     4141 tgttaattta tcataatccg taatctgtcc gacacgagta ttctcaacag tcagattcac
     4201 acgttcaacc ggtgtataga tcgagtcaat cgcaatcact ccgatcggca tatcttctgt
     4261 tttgttctta tcagaactta catatcctct gccctttgtg atcgtcaatt ccatattcag
     4321 cctgcagtct ttgccgccgc tcaatgtagc gatcacctga tccggattta cgatctcaat
     4381 atcagggtca ttaatattga tgtctgctcc tgtcacaaca ccttcgcctt caaactcaat
     4441 atatactgtt ttcggctcgt ctgactcaga catatttctt atcgcgagag atttcaaatt
     4501 catgataatc tccgtaacgt cttcttttac gcccggtatc gaactgaatt catgaagaac
     4561 tccgtcgatc ttaacggaac tgatcgcaga acccggaaga gatgagagca tgattcttct
     4621 cagagaattt ccgagtgttg taccatatcc tctttcaagc ggttctacaa nnnnnnnnnn
     4681 ccatattttt tatcttccga aacatccgta atctcaatat taggtttatt aaaatcaaac
     4741 actacacaga ccctcctttt gggttttaaa atgttgaagg gatacgctgt tgcagccgat
     4801 attatttaga atataactcg acaatcaaca tctcatctac cggaacatcg atcgcttctc
     4861 ttgccggaag ttcttttaca gatcctttca ggttctctgc atcaacatcg agccattccg
     4921 gaaccatgtt gctgccagtc gcttcaagaa tgtctttata tctctgtgag ccttttttgt
     4981 tttctttgat ctcaatcgta tctcctgctt ttacaaggta agacggaatg tttacctgtt
     5041 ttccgtttac gagaacgtgt ttgtgatcaa cgatctgtct tgcttctttt cttgttcttg
     5101 caagtcccat gcggaagatt acgttgtcca gtctggactc aagaattctc atcaggtttt
     5161 cacctgtcat tccttccatc tgttttgctt tcgcatagta gtttctaaac ggtttttcaa
     5221 gtacgccgta gatgaatttc gctttctgtt tctcgcgaag ctgcagaccg tactcgctca
     5281 tttttctgtt tgctcttttt aactgtctgt tagatttttt attaattccg aggtaaatcg
     5341 gatccatgcc taaagaacgg catcttttaa gaacaggaac tctgtttact gccatgtcta
     5401 tcctcctaat ttatacatac gagcggtttc ataccgcttt ccaagttact ttccggtgta
     5461 tgataatgtt cagccgtcga ttacactctt ctgcgtttcg gcgggcggca tccgttatgc
     5521 ggaaccggtg ttacgtcttt gatacttact acgtcaatac cgcatgcctg aagagcacgg
     5581 attgccgctt cacgtcctga tcccggtcct tttacgaata catctacaga cttaagtccg
     5641 tgtaccaatg ccgctttagc tgctgtctca gctgccatct gcgctgcata tggagtagat
     5701 tttcttgaac ctctaaatcc cagaccgcct gcacttgccc atgaaagagc atttccctgt
     5761 gtatctgtta atgtaacgat tgtattatta aaagatgact ggatatgtgc ctgtcctcgt
     5821 tcaacgtttt tcttgacacg tttttttgtt actttttttg caactttctt tgccatttaa
     5881 actaacctac tttcttcttt ctctttgatt taattccttg ttataacaag tttgttcgct
     5941 cagctaagct catgcttcgc atttgctaag atttgcgaac ggcttcgcac taaattcaaa
     6001 cttcgttttc attaagttgc tttcagctta ttttttcttg tttgctactg ttctcttcgg
     6061 acctttacat gttcttgcgt ttgtctttgt cttctgacca cgcaccggaa gtccttttct
     6121 atgacggatt ccacggtagc atccgatttc tttcagtctc ttgatgttaa gctggatctc
     6181 tcttctaaga tcaccttcta caacctgagt ttcatcgatc actgcgctga tcttttttac
     6241 ttcttcactt gtaagatctc ttacacgagt attcggatct actcctgcat ctgccaaaat
     6301 acggcttgcg cttgttcttc cgattccata aatataagtc aagccgatct ctacacgttt
     6361 gtctcttggt aagtctacac ctgcaatacg agccatgtgt ctttcctcca ttttctttgt
     6421 ttatactgtc tttaattgag gctgcggacg ctctgcttat tataatgccg catcctaccg
     6481 gccttttgcc gatatcccag cccagacgcg ttctcctcgc gccctttccc ggcacggatc
     6541 ttatccgtca atgcaaacgc cgggtattag aagcaatata caatcgcatc cgttcaagcc
     6601 atatgccggc tctgcctatg atcgtcacaa atggacgaaa cgggcaaact acggaagcgg
     6661 gacacacgca ctgccggtac ctgatgggca gatacggtgt aaccttactg tatattaaac
     6721 agctctacac acatcctcgt gtgtcatgct gtcagccgcc taaatcactt cacaaacttc
     6781 ctgtcaatta accctgacgc tgtttgtgtt tcggattttc acagattact ctgatgcttc
     6841 cttttctttt aataatcttg catttttcac aaattggttt tactgatgat ctaaccttca
     6901 tgtcaaatcc tccttccctt ctttgcttac ctacaatttc cggtcatttg ggcacacctc
     6961 accagaaatg accatttcat agtatatcac caaccccgca tttaagtcaa ccctctattt
     7021 cttatacttt cctatttctt tttcgctccg ctttttaatc gcaaatatta cgcaaaaaag
     7081 ttgttctgta cataagccct gactcttcgt cactgcagtc tcacagaaca acttcccgta
     7141 aatactcaaa ttccgcccgg ctttattgca ccggcttcct ttatgcttta tttatctctc
     7201 cagataattc tccccttgct aagatcatat ggcgacatct cgagcgtcac tttatctccc
     7261 ggaagaattt taataaagtt cattcttaat tttccgctga tatgtgcaag aacgatatgt
     7321 ccgttctcga gttccacttt aaacattgca ttcgggagct tctctactac tactccctcg
     7381 atttctatta cgtcagcttt tgacatatta tttaatcctc ctgaatttcg gcatcacatg
     7441 ccgtttcaca tttttactct cccagaatcc ttacaatttc cgcaaataca tcatcaatat
     7501 cgatcgttcc gtctacttcc tttaaaatac cggactgtgt ataataatcg atcaaaggct
     7561 gcgtctggtc atgatagacg ccaagtcttt tcagaactgt ttccggcttg tcatcgtcgc
     7621 gaagaatcaa accgcctccg catttatcgc aaattccttc ttctttcgta ggggcatata
     7681 cgagatgata tgttgccccg cagtctacgc aggctcttct tcccgacatt ctgtttacga
     7741 tattttcatc cggaacttct acgtcgatag cataatctat cttctgtccc atgtcggcaa
     7801 gagctttgtc taatgcttct gcctgcggaa tcgtacgcgg aaaaccgtca agcacatatc
     7861 cgttcgcaca atcatcctga ttcactctat ctacaacaag atcgactact aattcatccg
     7921 gcacaagaag accctgatcc atatatgtct tcgccttttt cccaagctcc gttccgtttt
     7981 tgatattggc acggaaaata tctcccgtgg aaatatgtgg aatcgaatat ttttctgcaa
     8041 tctttttagc ctgtgttcct tttccggctc ccggcgctcc cagcatgata atcttcataa
     8101 cttctacctc cgatcgatat gcaaacagtt cttttgcccc gaaatatttt cctgcataca
     8161 gaactgtttt ttgttttatc ttaaaataca ccttgagccc gctgcgtttt gcctttttca
     8221 aggcaaaaca gggcgagctg ttaaacattg ttatttaata tccaagaaag ctgtttgacg
     8281 cgttgcttcc tttattattt aagaatcctg tgtaatttct cacaagcatc tgcgactcaa
     8341 tcttcttgat cgtttcaagc acaacaccta cgataatgat aagcgatgtt cctccgaaag
     8401 aaacgtttgc gccgaacata ccattgaaga agaatgggat tacctgaacg ataatcagtc
     8461 cgcatgcgcc tatgaaaata atgtaattca aaattttctg caaatattca actgtcggtt
     8521 ttcccggacg aatacccgga acaaatccgc cgctcttctt catattattt gcaatctcca
     8581 aaggattaaa tgtaatcgat gtatagaaat atgcaaagaa gattgtgagc agaatatata
     8641 caagcagtcc aaccgtataa accggttttg ccggattaca ccagttgttt gaattcattg
     8701 cagcaaggat ctggcttccg atgcctgttc cgtttccttt tccgaggaac tgggcaatga
     8761 cgatcggagt ctgcatcaaa gatgatgcaa agataaccgg gattactccg gcagtgttta
     8821 cacgaagcgg aatatgtgta gactgtccgc cgtatgtttt tcttccctgt acttttttag
     8881 aatactgtac cgcaattcgt ctttctccgc tctgaagaag aacaacaaag actactactg
     8941 caagaattac cgctaaaata atcaatcccg caagacctgc ctgagcaagt ttctttccac
     9001 tcatgaattt ctcgtaaagt gtgtataaat cactcggaac acgagaaata atattgatca
     9061 gaagaacgat agaaataccg tttccgacac cgttttcagt gatg
//
LOCUS       DBMDXF010000067         8971 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2105_length_8971_cov_2.010823, whole genome shotgun sequence.
ACCESSION   DBMDXF010000067 DBMDXF010000000
VERSION     DBMDXF010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2105_length_8971_cov_2.010823"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(169..3351)
                     /locus_tag="ACZPD4_04115"
     CDS             complement(169..3351)
                     /locus_tag="ACZPD4_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1378291.1"
                     /translation="MITKFERKVIAGLTAFLMCLSMVFAVPVSVNAEAEQTQDEAVSD
                     AQQNEDVEDIQIPDSQEDVPHDITVGDEAGTITLKKDGTPEYGEAAFSTEQTTVRYRL
                     NVEEDGQYYFELIRDWSVNAYISIIEKKEDGDTWLPGEESRYELHKGGEYYLEITNNS
                     AESVQTLKWYAGKVRDIKPGEFEAELSENVKEIYYNLTVDETGWYRFDSGSLSMGIKD
                     KETEEFVSAWSFCKLESGHTYYITVSKSEVNPGTSSKWSVKKGKAISVELGQVNRNEN
                     SKEVYYVFVPETTGKYTVKEGASVYDENWKTLGIAYSVPLTAGKTYYFTVDRAQYWSI
                     QKYEESEKETIAVQTGTTYTTPFDKNREINYTFTPDTTAIYRFRSQQDNASLSIKECD
                     WQTGFGSSSGKIDFFIALEKGKTYEFSMVSLDKSEIQWNITKAGTKTANEGTEYTTTK
                     EETTVYDFVPSKSGEYMFSSKDGGTGKVYSSDWKEIEGYLYDGAAEFGMKVSLEQGKT
                     YHLGIALSDKEAKWKIEPVKESSDYTYRVLSDNTVEILKYSGVESNVTVPDKIDNKVV
                     KSVGYGAFAENENIVGVTIPAQVTDLQYGVFASCANLETVTFKEGSKLQKIAARAFEN
                     CSKLHSVSLPDSVQTIEENGFAHCKNLGPVDLGNGLKNIENNAFYYSGVTKVRIPGST
                     TEIGASTFYECRSLNDITLGSGLKGIESGVFYHCSNLKQIELPDNITYISDNAFGGSG
                     LTSVEIPDSVTSIGNLAFYGCQSLKKAVIGNNLAYIANNAFGSCALTEIMWGDKVEKI
                     GTESFSNNKNLTTVSLPNSITEIEYGAFNGCENLSDIEIPDSVEAIGGFAFDNEGSGI
                     RDTNTKWYDAQADGDVYAGKVYYKYKGEAPADTTVTIKDGTKGIAGYAFYMQGNVTEV
                     VIPNSVNNIGEVAFMGCESLKTVTIPESVKVIGREALGYLSSKQYEQGYKVEGFTIRG
                     VAGSAAEKYAKENGFTFEAMKPDYIKGDSDSDGKVTISDVRTTLRYVCQKVELDEEQK
                     LAADVEKDGVINIKDLRKVLRFVCNKIEEL"
     gene            complement(3470..4891)
                     /locus_tag="ACZPD4_04120"
     CDS             complement(3470..4891)
                     /locus_tag="ACZPD4_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1378292.1"
                     /translation="MNKKRIVGVALAVGACGVLAAAGMMLSKKSGAVVLDQAGNEIAK
                     LILQDGKISYECEDKYQSYADIVSQEAVKMIQEREGVNEKTAAKILADREITVKTWLD
                     RETLDAVIGSYNDGEGMACERFGAAVTDKEGHLTACYSDTKGNDNYNNVTKPAFAGST
                     MKPLSVYGPAIEDGTICWSSLYEDSAYGVVAGDNGIAREWPENVVPFTGKNVTAADAL
                     KESNNAVAVKVLKDYGVEKSVRFLEDTLGYDVDNEKKILEEEGEDRTLSNIASGYLAE
                     GEDRILSNIALGYLENGVTVSKMADAYQVFINEGKDTPLCAVKEIEKSGETYWTPQQK
                     ESEVFSEDTAYIMNRMLREVVTDGTGISAQVTGLDVCGKTGTSEYGDHWFVGMIPGNV
                     CAVWYKSDKDMDAETADAARICKGVFEKIGADASEQYSIPTGVKEEVYCKKSGLLAGE
                     QCQETGIGYYRKEDCPKVCGECK"
     gene            complement(4884..6554)
                     /locus_tag="ACZPD4_04125"
     CDS             complement(4884..6554)
                     /locus_tag="ACZPD4_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1378293.1"
                     /translation="MLILMTCIVIVGILGGGYYVSVSAVTGIVLTGVLFYRMYVKKRI
                     TAAWDLNMAAFAVLVFGYLLSCLWAVDSGMALMGVVKFLPLLLFYVLVSGLTDEREKM
                     IASLPMLGCLMTAFSFVMMQFEVFEQWVSVAGRLSGFFQYPNTYALFMLICLILVMWR
                     FDYKKIDWLDIVYGVAAVFGIIMSGSRTVFVLTAVAVIWVFAAKSSGKKVIISVLAAG
                     AVVAVILAVAAGSAGILERFTNISFGASTFLGRILYVQDALPLILKHPFGLGYYGYYF
                     IQQSVQTGVYTVVNAHNELIQILLDAGVIPAVFMGAAVLRSVFTKRTQSRNRIVLSIM
                     ILHSLFDYDFQFLAMGFVLILFLDMRNIKTQKVPVLTGTVVGLTGAAAAALSIMCGVS
                     DVCYTSGNYKAAEKVYSGNTMAQLALLTKADKAEEMKAIAEKVIVDNQSVSLPYSALA
                     QAAFADGDVEQFTEYKLKAIELAPYQYKEYENYLEVLVYCNDLYHQMGDKDGVRFCVE
                     KAEEIPKMLEQVKENTSALGWKIVDRPQVTLSHENLEIIEDMRRRMDE"
     gene            complement(6650..7477)
                     /locus_tag="ACZPD4_04130"
     CDS             complement(6650..7477)
                     /locus_tag="ACZPD4_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HIR1378294.1"
                     /translation="MRGKRIKQFILILCMVCLFGMNVQAEDVYTEFHVDNQKGKAGDV
                     VTVPVEFNTGQEVGGFQISIYYDKEVLEFQSLEAGDLIEKAGGGIFDYNHIEESSEII
                     VVYVVPDTVKNEGSIVDLKFKLKKDCTEKLPIGMKPDEVVDNTESSNLITGEVSGVDK
                     EFQEKVVGDLAVVSTDQNKSDRSKSDDKSDKKSDKDKDKSKSDKESSDDKKDSSESDE
                     KGKGDSGKKDADKADKDKKSKKLSAEDFKALAAFFGVMILSGVVVYLIKKKNGNKQK"
     gene            complement(7647..>8971)
                     /locus_tag="ACZPD4_04135"
     CDS             complement(7647..>8971)
                     /locus_tag="ACZPD4_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1378295.1"
                     /translation="VVKDNVTANSKTEIVGKDHGSYVGNTNENADPVPENTIPSKTVD
                     KNGKEIVNGRIPVVIELSSLKINKEDFTMTKGTSETLTVTGTPADALEGQTITWKSSN
                     KDVVTVDSEGKVEAVGVGNATITATAAGKSDSVKITVNNPLKKITVDPATLTLKKGTS
                     KTLSVKYDPADTTDNKAVTWESSDKSVATVDANGKVTALKDGSATITAKVGKLTATCA
                     LTVQEKKLTGISLDKTALELSKGQSSEALKVIYTPADTTDDKTVTWSSADEKIATVKN
                     GVVTAKATGTTKITATVGTHKAECTVTVNAKLTGIKVTPDKVTVEKGQKANLNVTYLP
                     ADTTDEKAVTWKSENESVATVDENGVVTAVKAGNVKITAKVGDKTAEYALKVTAKANG
                     TNNKNEKPADGGAVKTGDSNHISGFVVAMLLSIAAIVGVVIFKKRTHR"
BASE COUNT         2201 a         2392 c         1358 g         3020 t
ORIGIN      
        1 aggtcacgtt tttattatac acattaggag tttttatttc tgaatacacc gctaaagctt
       61 tcacggaacc acgcgactat cgcgtggttt tcttgataca acaaaactgc cgcttgcata
      121 taaaatcgca agcggcagtc tgtataattg aatctctttc aatcgctttt acagttcttc
      181 tattttgtta catacaaatc tcaatacttt ccttaaatct ttaatgttta taactccgtc
      241 cttttccaca tctgcggcaa gtttctgctc ctcgtccaac tcaaccttct gacatacata
      301 cctcaaagta gtacgaacgt ctgagatcgt cacttttccg tccgaatctg aatcaccttt
      361 tatataatcc ggcttcatcg cctcgaacgt aaatccgttt tcttttgcgt acttctccgc
      421 agccgatcct gctactcccc ggattgtaaa tccttctacc ttatatccct gctcatattg
      481 cttgctcgac aaatatccta aagcctctct tccgatcact tttacagatt cggggattgt
      541 gactgtcttc aaagattcac atcccataaa cgccacttct ccgatattat tcacgctgtt
      601 cggaatcaca acttctgtca catttccctg catatagaat gcatatcctg cgataccttt
      661 cgttccgtct ttgatcgtaa ccgttgtatc tgccggcgct tctcctttat atttgtaata
      721 aacttttcct gcatatacgt ctccgtcagc ctgtgcgtca taccatttag tattcgtatc
      781 tcttatgccg cttccctcat tgtcaaatgc aaaaccgccg attgcctcta ccgaatccgg
      841 aatttcaatg tcgctcagat tctcacatcc gttaaacgcc ccatactcaa tctctgttat
      901 gctgttcgga agcgaaactg tcgtaagatt tttattattg gaaaatgact ctgttcctat
      961 tttctcaacc ttatctcccc acatgatttc cgtcaatgca catgagccga atgcgttatt
     1021 cgctatatat gcaagattat ttccgattac tgcttttttc aatgattgac agccgtaaaa
     1081 tgccagattg ccgatacttg tcactgagtc cggtatctct acagacgtca atcccgagcc
     1141 gccaaatgca ttatctgaaa tataagtaat attatccggc agctcgatct gtttcaaatt
     1201 actgcaatga taaaatactc cggattcaat tccttttaaa ccacttccaa gagtaatgtc
     1261 gtttaacgat ctgcactcgt aaaatgtact tgctccgatc tctgtcgtgc tgccgggaat
     1321 acgaaccttc gtcactcccg aatagtaaaa cgcattgttt tcaatatttt ttaatccgtt
     1381 tccaagatct accggtccaa gatttttgca gtgagcaaat ccgttttctt cgattgtctg
     1441 aacgctgtca ggcagactga ccgaatgaag ctttgaacag ttttcaaatg ctcttgcggc
     1501 aattttctgt aatttgcttc cttctttaaa tgtaactgtt tccaaatttg cacatgatgc
     1561 aaatactccg tactgtaaat ctgtaacctg tgccggaatc gttacgccga caatattttc
     1621 attctccgca aacgctccgt aaccgacact tttaactacc ttattatcga tcttgtccgg
     1681 tactgttaca ttactttcta ctccggaata tttcaaaatc tctactgtat tatcagacag
     1741 cacacgatat gtataatcag atgactcttt taccggttca atcttccatt ttgcttcttt
     1801 atctgataac gcaatgccca aatgatatgt ttttccctgc tctaatgaaa ccttcatgcc
     1861 gaactctgca gctccatcat acaagtaccc ttctatttct ttccaatctg aactatatac
     1921 ttttcctgtt cccccatctt ttgaagaaaa catatattct cccgacttac tcggaacgaa
     1981 atcatatact gttgtctcct cttttgtcgt cgtatactct gttccttcgt tagcagtttt
     2041 cgtacctgct tttgtaatat tccattggat ctcactttta tccaggctta ccatcgagaa
     2101 ttcataagtt tttcccttct caagcgcaat gaagaaatct atcttgccac tcgaacttcc
     2161 gaatcctgtc tgccagtcgc attcctttat agacagactt gcattatcct gctgagatcg
     2221 aaaacgatat attgccgtcg tatccggagt aaacgtataa ttaatctccc tgttcttatc
     2281 aaacggcgta gtatacgttg ttcctgtctg taccgctatc gtttcctttt cactttcttc
     2341 atatttttgg atactccaat attgagccct gtccactgta aaataataag ttttccctgc
     2401 ggtcaacggt acagaatatg cgattccaag cgttttccaa ttttcatcat aaacacttgc
     2461 cccttctttt acagtatatt tccctgtcgt ttccggcaca aacacataat aaacttcttt
     2521 tgaattctca tttcggttaa cttgtcccaa ttctacagaa atcgctttcc cttttttgac
     2581 gctccattta cttgacgttc ccggatttac ctcgctctta cttacagtaa tataataagt
     2641 atgacctgat tctaatttac agaacgacca tgcggaaaca aattcctcgg tctctttgtc
     2701 tttaataccc attgacaaag aacccgaatc gaaacgatac catccggtct cgtccactgt
     2761 cagattgtaa taaatctctt ttacattttc gcttaattcc gcttcaaatt ctccaggctt
     2821 aatatctctt acttttcctg cataccactt taaggtttgt acagattctg cagaattatt
     2881 cgttatctcc agataatact ctccgccttt gtgcagttca tatcggcttt cttctcctgg
     2941 cagccatgta tcgccatcct ctttcttttc aataatagag atatatgcgt ttacactcca
     3001 atctctaatt aactcaaagt aatactgccc gtcttcctca acattcagac gataacgaac
     3061 cgttgtctgt tctgtcgaaa aagcggcttc cccatattcc ggtgttccgt cttttttcag
     3121 agtgatcgta ccggcctcat cacctacagt tatgtcatgc ggtacatcct cctgcgaatc
     3181 cggtatctgt atatcctcta catcctcatt ttgctgtgca tcacttactg cttcatcctg
     3241 cgtctgttct gcttctgcat ttacagaaac cggaactgca aacaccatcg acaggcacat
     3301 cagaaacgct gtaagtcctg cgatcacttt tctctcaaat tttgtgatca tactttttcc
     3361 ctcctttata ttttcaaaaa atgtattgtt ttctcttaaa gttaccgcct cctttcaagt
     3421 cagtttttac tgttaatatc tctttccgca aactctgcat actaatatat cacttgcatt
     3481 ctccgcatac tttcggacaa tcttctttcc tgtaatatcc gatacctgtt tcttgacact
     3541 gttctcctgc caaaagtccg ctctttttgc agtatacttc ttctttcaca ccggttggaa
     3601 tcgaatattg ctccgatgca tctgccccga ttttttcaaa gacccctttg catatccgag
     3661 cggcgtctgc agtttcagcg tccatatctt tatcagattt ataccatacc gcacatacat
     3721 ttcccggaat cattccgaca aaccaatggt ctccatactc gctcgttcct gttttcccac
     3781 acacgtcaag tcctgtcacc tgtgctgata tacctgttcc gtctgtcacg acttcccgaa
     3841 gcatccggtt cataatataa gccgtatctt cactgaacac ttccgattct ttctgctgcg
     3901 gcgtccaata ggtctctcct gatttttcta tttctttcac agcacaaaga ggcgtatctt
     3961 ttccttcatt tataaatacc tgatatgcgt ccgccatttt ggaaaccgtc acgccgttct
     4021 ccagatatcc aagcgctatg ttgcttaaaa ttctgtcttc tccttccgcc agatatccac
     4081 tcgctatatt gcttaaggtt cggtcttctc cttcctcctc taaaatcttt ttctcattat
     4141 ccacatcata accgagcgta tcttccaaaa aacgaaccga cttctcaaca ccgtaatctt
     4201 tcagtacttt cacggctact gcattatttg actctttcag tgcatctgca gccgtcacat
     4261 ttttccctgt aaacggcacg acattttccg gccattctct ggcaatcccg ttgtctcctg
     4321 ccacaactcc ataagcgcta tcttcgtaca acgatgacca acatatcgtt ccgtcctcaa
     4381 tcgccgggcc atatacacta agcggtttca tcgtcgatcc tgcaaacgcc ggctttgtca
     4441 cattgttata gttgtcgttt ccttttgtat cactgtaaca cgccgtcaga tgcccttctt
     4501 tatcagtaac cgccgctccg aaccgctcac atgccattcc ttcaccgtca ttataagagc
     4561 cgatcaccgc atccaaagtt tcccggtcga gccatgtttt cactgttatt tctctgtctg
     4621 ccagtatttt tgccgcagtt ttttcattca caccttcgcg ctcttgaatc attttgaccg
     4681 cttcctgcga aacgatatcc gcataagact gatatttgtc ttcacactca taactgatct
     4741 ttccgtcctg caatataagt tttgcaatct catttccggc ctgatcaagc acgaccgctc
     4801 cacttttttt gctaagcatc atccccgccg ccgcaaggac tccacatgct ccgacagcca
     4861 atgcgacccc gacaattctc tttttattca tccatccgcc tcctcatgtc ctcaatgatc
     4921 tcaagattct catgagacaa tgtcacttgc ggacgatcta cgatcttcca gcccagcgcg
     4981 ctcgtatttt cttttacctg ctccagcatc ttcgggattt cttctgcctt ttccacgcaa
     5041 aatcttaccc cgtctttatc gcccatctgg tgatacaggt cattgcagta tactaaaact
     5101 tcaagataat tctcgtattc tttatactga tacggcgcaa gttcaatcgc ttttaattta
     5161 tattccgtaa actgttctac gtccccatcc gcaaaagccg cctgtgccaa tgcactatat
     5221 ggaagagaca cagactgatt atccacgatc actttttccg caatcgcttt catttcctct
     5281 gccttatccg ctttcgtaag aagagccaac tgcgccattg tatttccact gtaaactttt
     5341 tcagccgctt tataatttcc cgacgtatag caaacgtccg aaaccccgca cataatcgaa
     5401 agcgccgctg ccgccgcccc ggtcagacct acaaccgttc cggttaaaac aggcactttc
     5461 tgcgtcttaa tattccgcat gtcaagaaac aggatcagca caaatcccat cgccagaaac
     5521 tgaaagtcat aatcgaacaa gctatgtaag atcataatgc taagaacgat cctgttccgg
     5581 ctttgcgtcc tcttcgtaaa tacagaacgt aaaaccgccg cgcccataaa taccgccggg
     5641 atcacgcctg catcaagcaa gatctgaatc aattcattat gtgcatttac aactgtatac
     5701 acaccagtct gtactgactg ctgtataaag taataaccat aatatccgag tccgaaagga
     5761 tgttttaata taagcgggag cgcatcctgc acatacaaaa ttcttccgag aaacgtactt
     5821 gctccgaagg aaatatttgt aaatcgctca agaatacctg cgcttcctgc tgcaacagcc
     5881 aatatcaccg ccacaacggc gcctgccgcc aacactgaaa taataacttt tttccccgag
     5941 gactttgccg caaacaccca tataacggca acagcagtta atacgaatac cgtacggctt
     6001 ccgctcataa taattccaaa tacagcggca acaccataca cgatatcgag ccagtcaatt
     6061 tttttgtaat caaatctcca cattacaaga atcagacaga ttagcataaa caatgcatat
     6121 gtattcggat attgaaaaaa tcccgacaac ctgcctgcaa ccgataccca ttgttcaaaa
     6181 acttcaaact gcatcataac aaatgaaaaa gcagtcataa gacatccaag catcggaaga
     6241 gacgcgatca ttttctctct ttcgtccgtc agacccgata caagtacgta gaacaataac
     6301 aacggaagga acttcacgac tcccataaga gccattcccg aatccaccgc ccataaacaa
     6361 gacagaagat atccgaacac aagcacggca aaggcagcca tattcagatc ccacgctgct
     6421 gtgatacgct tcttcacata cattctgtaa aacaaaactc cggtcagtac gattccggtt
     6481 accgcagaca ccgatacata atacccgccg cccaatattc cgactatcac tatacacgtc
     6541 attaaaatca gcatttttat cccgcccccg ctctcgcagc ttttacttgc ttttatttgt
     6601 ttatcgctat tttctgattt tatttttatc actgttttct gacttgtttt tacttttgtt
     6661 tgtttccgtt tttcttcttt atcaagtaaa caacgacgcc tgataaaatc ataactccga
     6721 aaaatgcagc cagcgcttta aaatcttcag ctgataattt tttcgatttc ttatctttat
     6781 ccgccttatc agcatctttt ttcccgctgt cacctttccc tttctcatct gattcggacg
     6841 aatctttttt atcatcgctt gattccttgt cagattttga cttgtctttg tccttatctg
     6901 attttttgtc cgatttatcg tccgacttcg atctgtctga tttgttctga tctgttgaaa
     6961 ctacggcaag atctcccaca actttctcct gaaattcttt atccacaccg gaaacttctc
     7021 ctgtaatcag gttactggac tccgtattgt ctaccacttc atccggcttc attccgattg
     7081 ggagcttctc cgtacagtct ttttttaatt taaacttcaa atcaacgata gatccttcat
     7141 ttttcactgt atccggaact acatacacaa cgattatttc tgatgattct tcaatatggt
     7201 tataatcaaa tattccgcct ccggcttttt caatcaaatc tcctgcctcc agcgactgaa
     7261 attccagaac ttctttgtca taataaatag agatctgaaa gccgccgact tcctggcctg
     7321 tattaaactc taccggaact gttacgacgt ctcccgcttt tcctttttga ttatctacat
     7381 gaaactccgt atatacatct tccgcctgaa cattcattcc gaaaagacag accatgcata
     7441 aaattaaaat aaactgtttt attctttttc ctctcatcaa cctctattct ccttacgctc
     7501 tttccctttt ataacatata ttttttgcac cttttcggag ctttttggta tcaaaagaaa
     7561 tacaatatca tctactgttt ttctttcttc tgataccaaa aagctccctg tcggtcagtc
     7621 gccaacaagg agctttcttt taacttttat ctatgcgttc tttttttgaa aattactacg
     7681 cctacaatcg ctgctatgga taaaagcatt gctactacga aacctgagat gtggttgcta
     7741 tctccggtct ttactgcacc gccgtcagcc ggtttctcat tcttattgtt tgtaccgttt
     7801 gcttttgctg ttactttcaa agcgtactca gccgtcttat ctccaacctt cgctgtgatc
     7861 tttacatttc cggcttttac agctgtaaca acaccatttt catcaactgt tgctacgctc
     7921 tcattttcgc tcttccatgt aacagctttt tcatctgttg tatctgccgg aagatatgtt
     7981 acattcaggt ttgctttctg acctttttca actgtcacct tgtccggtgt cactttgatg
     8041 cctgtcaatt ttgcattaac cgtaactgta cattcagctt tatgtgtacc tactgttgcc
     8101 gtaatctttg ttgtgcctgt tgctttcgct gtaacgacac cattttttac tgttgcgatc
     8161 ttttcatcgg cgcttgacca tgtaactgtc ttatcatctg ttgtatctgc cggtgtataa
     8221 atcactttaa gagcttcact gctctgacct ttggaaagtt ccaaagctgt tttatcaagt
     8281 gaaattcctg tcagtttctt ctcctgtact gtaagtgcac aagttgctgt aagttttccg
     8341 acctttgcag taattgttgc gctgccgtct tttaatgctg tcactttacc gtttgcatca
     8401 actgtcgcta cgctcttatc gctgctctcc catgttactg ctttgttatc tgtcgtatct
     8461 gccggatcat atttcactga caatgtcttt gaagtacctt ttttcaatgt aagagttgcc
     8521 ggatctacag taattttttt caacggattg tttactgtga tctttacact gtcgctcttg
     8581 cctgctgctg tcgctgtaat tgttgcgttt ccaacgccaa ccgcttcaac tttaccttcg
     8641 gaatcaactg tcacaacatc tttattggaa ctcttccatg tgatcgtctg accttccaaa
     8701 gcatctgcag gtgttcccgt tactgtaagc gtctcggaag tacctttcgt cattgtaaag
     8761 tcttctttgt tgatcttaag agaagaaagt tcgattacaa ccgggattct gccgttcaca
     8821 atttcttttc cgtttttatc cactgtttta gacggaatag tattttccgg aacagggtca
     8881 gcattctcat tcgtatttcc tacgtaacta ccatgatctt ttccgactat ttctgtcttg
     8941 ctatttgcag tcacattatc tttaactact a
//
LOCUS       DBMDXF010000068         8961 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2109_length_8961_cov_1.896501, whole genome shotgun sequence.
ACCESSION   DBMDXF010000068 DBMDXF010000000
VERSION     DBMDXF010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_8961_cov_1.896501"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            81..710
                     /locus_tag="ACZPD4_04140"
                     /pseudo
     CDS             81..710
                     /locus_tag="ACZPD4_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
     gene            971..2383
                     /locus_tag="ACZPD4_04145"
     CDS             971..2383
                     /locus_tag="ACZPD4_04145"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1378296.1"
                     /translation="MGGFFGVASKKDCVLELFYGVDYHSHLGTRRGGMAAYGNGEFCR
                     AIHNIENAPFRTKFDRDVDEMKGNLGIGCISDYEPQPLLIQSHHGSFAIVTVGKINNE
                     EELLEKVFEEGHSHFQEMSGGKINATELVASLICKKETLVEGIRYAQETVNGSLTLLL
                     MTKDGIYAARDRYGRTPVVVGKKEEAYCVSFENSAYINLGYEAYKELGPGEIDLITPE
                     SVEQLVAPGKEMKICSFLWVYYGYPTSSYEGVNVEEMRYKCGSMLAKRDGDSVHPDIV
                     AGVPDSGIAHAIGYSNESGIPYARPFIKYTPTWPRSFMPTSQSQRNLIAKMKLIPVQS
                     LIEDKKLLLIDDSIVRGTQLRETTEFLYKSGAKEVHVRPACPPLVYGCKFLNFSRSKS
                     DLDLITRRIIQEAEGENCPAEVLAEYADPDSCRYAKMVEEIRKIQNFTTLRFHRLDDL
                     IASIGINPCKVCTYCFNGKE"
     gene            2454..2767
                     /locus_tag="ACZPD4_04150"
                     /pseudo
     CDS             2454..2767
                     /locus_tag="ACZPD4_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242255.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     assembly_gap    2768..2777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2778..3832
                     /locus_tag="ACZPD4_04155"
     CDS             <2778..3832
                     /locus_tag="ACZPD4_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378297.1"
                     /translation="VTALFTVSALAVPELLMQVFTNDPVVVSEGVKYLRIVAFTYIMM
                     GVTQAYLYIMRSVERVVVATVVYLLSLICNIIMNAIFIFGLLGLPKMGVAGAALGTLC
                     ARILEVVLVAIYARLFNREIRLRFKYVLHTDKILFSDFMRYALPVVVNEVLWGLGTAA
                     NTAILGHMGSAAVAANSVAQVARQLATVVSFGLSSAAAIYLGKTIGEKKTEYAKAYAK
                     RFLILSIIMGILGGTVILAVSPLASAALTLSGTAKEYLRIMFFVMSYFVVGQAINTTL
                     VVGVFRSGGDTRFGLILDMTTMWCCSILFGFLAAFVFKLSVPVVYVILMSDEIIKIPI
                     TYKRYRSYKWLNDVTR"
     gene            3979..4809
                     /locus_tag="ACZPD4_04160"
     CDS             3979..4809
                     /locus_tag="ACZPD4_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1378298.1"
                     /translation="MPEQQNTNGCTEESCAGCAHADSCSSKKEDFREPENKYSSIKKV
                     IGVVSGKGGVGKSLVTASLARMMREKGYTVGILDADITGPSIPKMYGIHEKARGTEEG
                     ILPCIAKDETRIMSVNLLLEDESAPVIWRGPIIAGVVKQFWTDVMWGDIDYLFVDMPP
                     GTGDVPLTVFQSLPVDGVVIVTSPQDLVQMIVKKAANMAEQMNIPVLGIIENYSYVKC
                     PDCGKEIKVFGESHVEEAAREMNVPVLGRMPIDMKLAEAVENEKFYEAENPYLADVKL
                     "
     gene            4881..5831
                     /locus_tag="ACZPD4_04165"
     CDS             4881..5831
                     /locus_tag="ACZPD4_04165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1378299.1"
                     /translation="MIGKRTAIWKREEYSYESEERDIPYVISYMHEDGQIRPAMIVVP
                     GGAYRQVSSSEGDIVARTFYRAGYNALVLTYSVNPTSEGEPLHLQPLRDLARTVRLVR
                     YYAKQCRIDPEKVAVCGFSAGGHLCGSLGVHFQDIKEENSLLKEVSCRPDAMILGYPV
                     ITSGKYRNEDSFLALLGEDSDEEEREYLSVEKHVTEEMPPCFLWHTLEDKTVSAENGY
                     LFAEACRKAGVPYAHHVFAEGAHGMSVATEEWFEQKLKERPDKWTEEEQAHIYGALDE
                     VGMWTGLAKRWLKRTLCIKERQEIDSEDFIYQTWRSGLHK"
     gene            5785..6558
                     /locus_tag="ACZPD4_04170"
     CDS             5785..6558
                     /locus_tag="ACZPD4_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1378300.1"
                     /translation="MRILFIRHGDPDYINDTLTKKGHREAKLLAERAADLNLGSCYVS
                     PLGRAKDTAQYCLDKVGKTAQTVEWLREFPARIDLNQAVHLQKAYPTAEKENGRYVPR
                     IVWDIVPSYWMEHGECMDRDAWRKSDLCQNSDAVEVYDRVTLEFDRFLAEHGYVREGS
                     SYRVERECTETVTFFCHFGITCALLSHLWNMSPFSAWQYFAFAPTSVTEIVTEEREKG
                     IACFRGLKLGDASHLYAGNEPVSVAARFCEVYSDMNSRH"
     gene            6733..8367
                     /locus_tag="ACZPD4_04175"
     CDS             6733..8367
                     /locus_tag="ACZPD4_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1378301.1"
                     /translation="MISANNVTLRVGKKALFEEVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGQLEPTNGEVAITPGERLSFLQQDHFKYDEYPVLDTVIMGNARLYEIMKEKEAI
                     YAKEDFTDEDGIKASELEGEFATMNGWEAESDAASLLNGLGIETDLHYKMMKDMTGPQ
                     KVKVLLAQALFGNPDILLLDEPTNHLDLDAIAWLEEFLINFENTVIVVSHDRYFLNKV
                     CTQIADIDYGKIQLYAGNYDFWYESSQLLIRQMKEANKKKEEKIKELQDFISRFSANA
                     SKSKQATSRKRALEKIQLDEIKPSSRKYPYIDFRPNREIGNEVLTVEGLSKTIDGVKV
                     LDNISFTLGHDDKVAFVGSNEVAKTVLFKILIGEMEPDEGTYKWGVTTSQAYFPKDFG
                     GEFDSDYNITEWLTQYSEEKDVTYVRGFLGRMLFSGEDGVKKLKVLSGGEKVRCLLSK
                     MMISGANVLLLDEPTNHLDMESITALNNGLIKFPGVLLFTSRDHQIVQTTANRIMEIV
                     PGGQLIDKITTYDEYLESDEMARKRQTYTVSTEEDD"
     gene            8461..8546
                     /locus_tag="ACZPD4_04180"
     tRNA            8461..8546
                     /locus_tag="ACZPD4_04180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8496..8498,aa:Ser,seq:cga)
     gene            8736..>8961
                     /locus_tag="ACZPD4_04185"
     CDS             8736..>8961
                     /locus_tag="ACZPD4_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1378302.1"
                     /translation="MTDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVHARQEGKWTHYSLSK"
BASE COUNT         2688 a         1520 c         2381 g         2362 t
ORIGIN      
        1 ggcaggattg gcaagtagag aatccaatac cgtcagcgtt ttaccgtttc tgtggtaatc
       61 cttgtttacc tgtcgggcat tcagttcaaa atgtgcagac atgtcctaac tgcggaggct
      121 ccggtaaagt gattaaggaa aaatgttcaa aatgcgcagg aagcggatac attgcaaacc
      181 gaaagaagat caaagtgtct atcccggcag gaattgataa cggccagagc gttcgtatca
      241 gagacaaggg agaaccggga gtaaacggcg gaccacgggg agatcttctt gtagaagtga
      301 atgtttcggc gcatccgtcg ttaaaacgac aagatattca tattttctct acgcatccga
      361 tttcatttgc ggtagcggcg ctcggaggag atattcgtat tcctacggta gacggagatg
      421 tgatttataa tgtaaaaccg ggaacgaaaa cagatacaaa agtgcgcctt aaaggaaaag
      481 gagttccgtc tcttcgtaat ccacaggcaa gaggagatca ttatgtgacg cttgagatcc
      541 agactccgga aagattaagt tcggaggcga aagaagcgct gcgaagattt gatgaattga
      601 cgggaaatac acttcatgaa catgatgaga caacagggaa agtcgagaag aagaaaaaga
      661 aaggcttcat ggaaaaaatg aaagagatga aagaagcctt ggaagactaa caggtaagag
      721 gatgtcaaaa taaggcatcc tcttattgta tagacggaga aaatgggaat cgtttgcagg
      781 gagaatgatt ttgatgccaa agcggcaaaa ataaaggaat agaacttctg cttatatata
      841 tctataataa acgataatag caggcatatg aaagaaagta ttgacttatc gccgggaact
      901 ccttatcctt tataaggtga aagataaata gcagaatgaa tttgataaat ttacaggagg
      961 cacatagaaa atgggtggat ttttcggagt tgcatcaaaa aaagactgtg tattggagtt
     1021 attttacggc gtggactatc attctcacct cggaacgaga agaggcggaa tggctgcgta
     1081 tggaaacgga gagttctgcc gtgcgatcca taatatagaa aatgctcctt ttcgtacaaa
     1141 atttgacagg gatgtagatg agatgaaagg caatcttgga atcggatgta tttctgatta
     1201 cgagccgcag ccgcttctta tacagtcgca tcacggaagt tttgcgatcg ttacggttgg
     1261 aaagatcaat aatgaagaag aacttttaga aaaagtgttt gaagaagggc attcccattt
     1321 tcaggagatg agcggaggta agatcaatgc gacagagctt gtggcatctt tgatctgtaa
     1381 gaaagaaacg cttgtggaag gaatccgtta tgcgcaggaa actgtaaacg gctctcttac
     1441 gctgcttctt atgacgaaag acggaattta tgcggcgcgt gacagatatg gaagaactcc
     1501 ggttgttgtc ggtaagaaag aagaggcgta ctgtgtttct tttgaaaatt cagcgtatat
     1561 taatctcggt tatgaagcgt acaaagaatt agggccggga gagatcgatc tgattactcc
     1621 ggaaagcgtg gaacagttgg tagctccggg aaaagagatg aagatttgtt cttttttatg
     1681 ggtttattat ggttatccga cttcctctta cgaaggcgtc aatgtagaag aaatgcgtta
     1741 taaatgtgga agtatgcttg caaagcgcga tggagattct gtgcatcctg atattgtagc
     1801 cggagttcct gattctggga tcgcacatgc gatcggatat tctaatgaat cgggaatacc
     1861 gtatgcgaga ccgtttatta aatatacgcc gacgtggccg aggtcattta tgcctacaag
     1921 ccagagtcag agaaatctga tcgcaaagat gaagctgatt ccggtgcagt ctctgatcga
     1981 agataagaaa ctgcttttga tcgacgattc tatcgtgcgc ggaacgcagc ttcgggagac
     2041 gacggaattt ctctataaga gcggagcgaa agaagtgcac gtacgtccgg cgtgtccgcc
     2101 gcttgtgtac ggttgtaagt ttttgaattt ttcaagatcc aaatcagatc ttgatctgat
     2161 cacacgcagg atcattcaag aggcagaagg agaaaactgt ccggcagaag tgcttgcgga
     2221 atatgccgat ccggacagct gccgttatgc gaagatggta gaagagattc ggaagatcca
     2281 gaattttacg actcttcgtt tccaccgtct ggatgatctg attgcatcga tcggaatcaa
     2341 tccgtgtaaa gtgtgtacgt attgctttaa cggaaaagaa tgatcgaaaa acagctgcag
     2401 aatcattaca gggcagcaaa agaataagta aaaggtagtt tacggaaaat atcatgctaa
     2461 agagagaaaa tcatatagaa attgaccgga gaaagtttta caaaacggtg ctggcgctcg
     2521 ttgttcctat ggctcttcaa aatctgatca acgtaggcgt gacggcggca gacgtcatta
     2581 tgttaggcgc tgttggagaa aaagctttat ccggcgcatc tttggcagga cagattcagt
     2641 atattatgac attgttcctg ttcggtctga cctccggagc gacggtgctc acggctcagt
     2701 attggggaaa aggcgaccgg gatgcgattg aaaagatatt aggaatggca gtaaaagcag
     2761 cggtctgnnn nnnnnnngtg tgacggcgct ttttactgtg tcggctcttg cggtaccgga
     2821 actgttaatg caggtattta caaacgatcc ggttgttgtt tcggaaggag ttaaatacct
     2881 gagaattgtg gcgtttacct atataatgat gggagttacg caggcatatt tgtatattat
     2941 gcggagtgtg gaacgggttg ttgtggcaac agttgtctat cttctgtctc ttatatgcaa
     3001 tatcataatg aatgcgattt tcatttttgg tctgctcgga cttccgaaga tgggtgtggc
     3061 aggagcggcg cttggaactc tttgcgcccg tattctggaa gttgtgcttg ttgcgatata
     3121 tgcccgttta tttaacaggg agatcaggct gaggttcaaa tatgtgcttc atacggataa
     3181 aattctattt tctgatttta tgcgttatgc ccttccggtt gtagtcaatg aagttttatg
     3241 gggacttgga acggcggcga acacagcgat actcggtcat atgggaagcg cggctgtggc
     3301 ggcaaattca gtggcgcagg tggcaagaca gcttgcgaca gttgtatcgt ttgggttgtc
     3361 cagcgcggcg gcgatctatc ttggaaagac gatcggagag aaaaagacag agtatgcgaa
     3421 agcatatgca aaacggtttc ttatattgag cattatcatg ggaatattgg gaggaactgt
     3481 gattcttgcg gtttcgcctt tggcatcggc ggcactgact ttgagcggta cggcaaaaga
     3541 atatttgagg atcatgtttt ttgttatgtc ttattttgtt gtcggccagg cgattaatac
     3601 aacgcttgtc gtgggggtgt tccgttcggg cggcgatacg agattcggat tgattctcga
     3661 tatgacgacg atgtggtgct gctcgatatt gttcggtttt cttgcggcat ttgtatttaa
     3721 gctaagtgtg ccggttgttt atgtgatact tatgagtgat gagattatta aaataccgat
     3781 cacatataaa cggtatcgaa gttataaatg gctcaacgat gtgacaagat aacagtggaa
     3841 cggttgtaaa cagacgttaa atgaatatga ataaatgatt aataaatgag aaatgatatt
     3901 cgggcaaaat cagtacaaga ttttgcccga atgtgttata cttagaaaag agtaaattat
     3961 agaaagaggt agcagatcat gcctgaacag caaaatacaa acggatgtac agaagaatct
     4021 tgcgcaggat gcgcacatgc agattcatgc agcagcaaaa aagaggattt ccgtgaaccg
     4081 gaaaacaaat attcatctat taaaaaagta atcggtgtag tcagcggaaa gggcggtgtc
     4141 ggtaaatcgc ttgtgacggc gtcgctcgca agaatgatga gagaaaaagg atatacagtc
     4201 ggaattttag atgcggatat tacaggtccg tctattccga aaatgtacgg aatacatgag
     4261 aaagcaagag gaacggaaga agggattctt ccatgtattg caaaagatga gacaagaatt
     4321 atgtcggtca atcttctcct ggaagacgaa tccgcgcccg ttatttggag aggtccgatt
     4381 attgcgggag ttgtgaagca gttttggaca gatgttatgt ggggcgatat tgattatctg
     4441 tttgtagata tgccgccggg aacaggagac gttcctttga cagtatttca gtcgcttccg
     4501 gtagacggtg tggtgatcgt gacatctcca caggatcttg tacagatgat cgtgaaaaaa
     4561 gcggcgaata tggcggaaca gatgaatatt ccggtacttg ggatcataga aaactacagt
     4621 tatgtaaaat gccctgactg tggaaaagag attaaagtgt tcggagaaag tcatgtagag
     4681 gaagcagctc gggagatgaa cgttccggtg cttggccgta tgccgattga tatgaagctg
     4741 gcagaggcag ttgaaaatga gaagttttac gaagcggaga atccgtattt ggcagatgtc
     4801 aagttataag aatcatcctt gcgccggcaa ggtatattgt tgaaaatgtt tttgccgatc
     4861 ggtataaaag aggtggaaaa atgataggta agagaacggc aatttggaaa agagaggaat
     4921 attcgtatga aagtgaagaa agagatattc cttatgtgat aagttatatg catgaagacg
     4981 ggcagatccg tccggcaatg atcgttgtgc cgggcggagc ctatcgtcag gtgtcatcat
     5041 cggaaggcga tatcgtcgca cgaacatttt atagggccgg atacaatgcg cttgtactta
     5101 catacagtgt gaatccgaca tcggaaggcg agcctttgca ccttcagccg cttagagatc
     5161 tggccagaac ggtgcggctc gtacgttatt atgcgaaaca gtgccggatc gatccggaga
     5221 aagtggctgt atgcggattt tcggcaggcg ggcacttatg cggaagtctc ggagttcatt
     5281 ttcaggatat aaaagaagaa aattctcttc ttaaagaagt ttcatgcaga ccggatgcga
     5341 tgatactcgg ttatccggta atcacttcgg gaaagtatag aaacgaagat tcgtttttag
     5401 ctttgctcgg agaagattct gatgaagaag agcgggaata tctgtcggtc gaaaaacatg
     5461 tgacagaaga gatgccgccg tgttttttgt ggcatactct tgaagataag accgtttcgg
     5521 cggaaaacgg gtatttgttt gccgaagcat gcaggaaggc gggcgtacct tatgcgcatc
     5581 atgtatttgc ggaaggggca catgggatgt ctgtcgccac agaagaatgg tttgaacaaa
     5641 aactaaaaga acgtcctgat aaatggacag aggaagagca ggcgcatata tatggcgcat
     5701 tagacgaagt ggggatgtgg accggacttg caaaacgatg gttaaaacgg acgctttgca
     5761 taaaagaaag gcaggagata gacagtgagg attttattta tcagacatgg agatccggat
     5821 tacataaatg atacgctgac gaaaaaagga catcgtgagg caaaactgct tgccgaacgg
     5881 gcggctgact tgaatctcgg aagctgttat gtttctcccc ttggaagagc gaaagacacg
     5941 gcgcagtact gtctggataa agtgggaaag accgcacaga ctgtagaatg gctgagagaa
     6001 tttccggcaa ggatcgatct gaatcaggcg gtacatcttc aaaaggctta tcctaccgcg
     6061 gagaaagaaa atggtcggta tgtgccgaga atcgtgtggg atatcgttcc gtcgtattgg
     6121 atggaacatg gagaatgtat ggacagggat gcgtggagaa aatccgatct atgccaaaat
     6181 tcagatgcgg tggaagtata tgacagggta actcttgagt ttgacagatt tcttgcagag
     6241 catggatatg ttcgtgaagg cagcagttat agagtggaga gagaatgcac ggagaccgtg
     6301 acgttttttt gccattttgg gattacgtgc gcgttgttgt cgcatctgtg gaatatgtcg
     6361 cctttttcgg catggcagta ttttgcgttt gctccgacat cggtgacgga aatagtgaca
     6421 gaagaacgag agaagggaat cgcttgtttt cgggggttga agctgggaga tgcttctcat
     6481 ctttatgcgg gaaacgaacc ggtttccgtt gccgccaggt tttgtgaagt atacagcgat
     6541 atgaacagca ggcattgaga gaacagctta aaattatagg agctttcgga aacagaaggc
     6601 agggattggg gaaacgtaca tgtttcctat aagtccttgc ctttttcatg gattatatgt
     6661 taatataggc gttggtgcgt attaagcatc acatatcccg taaaacgaag tttcaaaaaa
     6721 ggagatattg taatgatcag tgcaaataat gtaactctgc gcgtgggaaa aaaagcgctt
     6781 tttgaagaag taaatatcaa gttcacagag ggaaactgtt atggtctgat cggcgctaac
     6841 ggagccggaa aatctacgtt tttgaagatt ttatcaggtc agcttgagcc gacgaacggt
     6901 gaagtggcga tcactccggg agagcgtctt tctttcttac agcaggatca ttttaaatat
     6961 gatgaatatc cggttcttga tacggtgatc atggggaatg cccgactgta tgagattatg
     7021 aaagaaaaag aagcgattta tgcaaaagaa gatttcacgg atgaagacgg aataaaggca
     7081 agtgaacttg aaggtgaatt tgctacgatg aacggatggg aagctgaatc agacgcggcg
     7141 tctcttttga atggtcttgg aatcgagaca gatcttcatt ataaaatgat gaaagatatg
     7201 acaggtccgc agaaggtgaa agtgcttctc gcacaggcgc tgttcggtaa tccggatatt
     7261 ttgcttcttg acgagccgac aaaccacctt gatctggatg cgattgcgtg gcttgaggag
     7321 tttttgatta attttgaaaa tacggtgatc gttgtgtctc atgaccgtta tttcttaaat
     7381 aaagtatgta cgcagatcgc agatattgat tatggaaaaa tacagcttta tgcgggaaat
     7441 tatgatttct ggtatgaatc aagtcagctt cttatccgtc agatgaaaga agcaaataag
     7501 aaaaaagaag agaagatcaa agaacttcag gactttattt cccgattcag cgcaaatgct
     7561 tcaaaatcaa agcaggcgac atcgagaaag cgcgcccttg agaaaatcca gcttgacgag
     7621 atcaaaccgt ccagcagaaa atacccgtat attgattttc gtccgaatcg tgagatcgga
     7681 aacgaagtgc ttacggtaga aggtctttct aaaacgatcg acggcgtgaa agtgcttgac
     7741 aatatcagct ttactctcgg tcacgatgat aaagtcgctt ttgtcggaag taatgaagtc
     7801 gcaaagacag ttctttttaa gatcctgatc ggagagatgg aaccggacga aggaacttat
     7861 aaatggggag tgacaacttc tcaggcatat ttcccgaaag atttcggcgg agaatttgac
     7921 agtgattata atatcacaga atggctgaca cagtattccg aagaaaagga tgtcacatat
     7981 gttcgcggat tcctcggaag aatgctcttc tcaggcgagg acggtgtgaa gaagctgaaa
     8041 gttctttcag gaggagaaaa agtacgctgc cttctttcta agatgatgat ttcaggagca
     8101 aacgttcttc ttcttgatga gccgacaaac catcttgaca tggaatcgat cacagctctt
     8161 aataatggac tgatcaaatt cccgggagtt ttacttttta cgtctcggga tcatcagatc
     8221 gtacagacga ctgcaaaccg tattatggaa atcgttccgg gtgggcaact gatcgacaag
     8281 attacgacat atgacgagta tcttgaaagt gacgagatgg caagaaagag acagacatat
     8341 actgtttcga ctgaggaaga tgattaaagg ttgattaaat ttgaaatatc ctctttcttt
     8401 ttggaaataa ttctgttata atgtaaaagc tgtgtatgga aatcatgcac agctcttcat
     8461 ggagacgtat cgaagtggtc ataacggggc gcactcgaaa tgcgtttgcc ctccggggca
     8521 cgagggttcg aatccctccg tctccgtttg aaaaatgaag ccgagagtca gaatgcagtt
     8581 gactctcggt ttattttatc tttttctttt tttgtctttg gcagacaaga ggaagaaaaa
     8641 acacatcaaa atattttgat gtgacatgtt gacaaaccac tttacaagac ttatgatgta
     8701 tatatcaaaa ataattgatg tgaggaatga cggcgatgac tgatttttca aaagacgcaa
     8761 aaatctttaa ggctctttgt gatacaaaaa ggcttactat tttagattac ttgaagagtg
     8821 gcgaaaaatg tgcgtgtgtt ctgattgaaa acatgaacat cggacagtca gctttatctt
     8881 atcacatgaa aatactttgc gattccggca ttgttcatgc aagacaagag ggaaaatgga
     8941 cacattacag cctaagtaaa a
//
LOCUS       DBMDXF010000069         8887 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2123_length_8887_cov_2.182222, whole genome shotgun sequence.
ACCESSION   DBMDXF010000069 DBMDXF010000000
VERSION     DBMDXF010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_8887_cov_2.182222"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="ACZPD4_04190"
     CDS             <1..1560
                     /locus_tag="ACZPD4_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="HIR1378303.1"
                     /translation="VSDLIALQAPNSYYLKDSMEEVKQNVTDFNQNIIDYEAQHKSEA
                     NGELADLLQSLFATLTEYYTNGTNVTSYQSGDCFGNSHMPELCQHVLTANEYLKENAE
                     EIELAEVKMQEVFAQQYEDACKAREEEGAIKLLTGGAAAITGILAIVGTGGMATPVVI
                     GVWVTGGSTALYGASKAIEGIQDVHYGRAKDLTSKSFNFIRDTVFQGNQEAYDAWGNI
                     NMMVAGLLVPIGQGTKAGAGLGVKAIARESAKRVAKEAVKDMAVDFTAETMTNWAREE
                     LDLGDFQTELLKTGLEFGLDKGLDKVGSKHVPFTDDMTFSEAKRYNQSMQNMESGKHN
                     PPGLSDADMDAWDFANQKVSEHIAVKKVDHNEVIKIRSEYAKKQESFLHPKTDADSAK
                     TATFTNQEAEDLAFGAIKGKGKSDAVVLGKFEDGKSTSYDKIAQEYDAQYYNLDEWDE
                     LAKTHSRDEMWKVNEKFLDIEIASGRDIYLSHDPAKFSGDGSFFAKEIEYLRQHGYKF
                     VKEGDLWHAVQ"
     gene            1643..2155
                     /locus_tag="ACZPD4_04195"
     CDS             1643..2155
                     /locus_tag="ACZPD4_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378304.1"
                     /translation="MKTRDEKEKKRDLFTTIFWILVLVFLIVKIVIPETGVLGMVNEG
                     SNAVLFFGIGGSCVWTIIHKRKTNKTAVVFGVLLLVLTLGIGGWISGKLVLDLISGPE
                     VVRLTSVTMRESSGIYGLISRHYYLCGRDDEWTEYQMEISSDEYYELEGEEQVTVLCY
                     PRTERILKFL"
     gene            2213..2668
                     /locus_tag="ACZPD4_04200"
     CDS             2213..2668
                     /locus_tag="ACZPD4_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378305.1"
                     /translation="MGSSQYEYMERKRMETEQKEMMCKYKKIICKIFGEQIRVIGESS
                     AIGPMGQFQIRFFYEPTKIYVTLDADRGAFTFDLKDEAKDWNTLYRIKKFDNCMTEKC
                     LENAAVILKQVLEENKFPLYKSENDKLYKKQDGTYRRIKDIYAELAGGE"
     gene            2673..3140
                     /locus_tag="ACZPD4_04205"
     CDS             2673..3140
                     /locus_tag="ACZPD4_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378306.1"
                     /translation="MAIKEVSERYLELRQNALDYTFEQMNLQLENDKQVYLAVFDIPV
                     ESAIIGNKTKTLVLVFGLNIHIYCANGDAVTGLEQNAKAKQAMQSLFISCPQALDEMT
                     LTHKTDFYESKNVRAYLKTRKGVYFKELTGETKKERFLEMLMRNVTEEVNFRH"
     gene            3712..4749
                     /locus_tag="ACZPD4_04210"
     CDS             3712..4749
                     /locus_tag="ACZPD4_04210"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1378307.1"
                     /translation="MKDKINSILSEAKGKISDAVSEGELQNVKSAYIGKQGSLSLLMK
                     EIPTVDVALRPEMGKLLNQAKNEVKDLIDEKRMELKLKASEVSPDFDCSVPGILPTGG
                     GLHPITQMCYDLNDTFRSMGFEVFEEDEITSEMYAFDKLNFPPNHPARESMDTYWLEG
                     HDSGSTNEKLCLRPHLTGGSVRYMQTHKPPYRFVYPGRVYRNETTDAHHERAFFQYEA
                     LIVDKDITFTSGKVMIKSILSKVFGTDVPVRMRSGFFPFVEPGFEIDMQCQVCGGKGC
                     SVCKHVGWIEVMPGGSPHPNVLRAAGLDPDEYTGFYVNIGLDRLVMMRYGVDDVRLFH
                     SADLRFLKQFR"
     gene            4778..7222
                     /gene="pheT"
                     /locus_tag="ACZPD4_04215"
     CDS             4778..7222
                     /gene="pheT"
                     /locus_tag="ACZPD4_04215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1378308.1"
                     /translation="MKLSLSWIRDYVNIPEDADLKKLAYDLTMSTVEVEDVEYLAERF
                     ENMIVGVIEKIEPHPNADRLRVCKVNIGSDEIKDIVCGGINLEEGMRVAVSCPGAVVR
                     WHGEGEPVVIKNSKLRGVESFGMICASDEIGLGELFPSETEGEILDLSAFDVPAGTSL
                     ADALDMNDVLLEIDNKSMTNRPDLWGHYGIAREIAALYNLPLAKIEPFRADVQSDFKV
                     EIEDPDRCTRYIGVEMSGVAVKPAPYKMQNRIWKVGMRPINALVDITNYVMLATGNPT
                     HAFDADNITDHIVVRHAGEGEKLLLLNDKELELNADDLVITDSEGAVALAGVMGGAKD
                     SILPKTERVILEVANFEATGIRRTALRYDTRTEASSRYEKAIDPERCDQALALSMKYF
                     KELYPELQVTGYCDNYVKKLDRAEIDVNLTWLEKRLGKHLTNEEIQGKLEQLGFDVQI
                     DGDNMHVTAPTWRSTGDISIKDDVMEEVARMYGYDNFEATEFTTTFTDSINQKDKSLV
                     RNIKEYLAIRCGMQEVYTYPWMNDVFVNAVLQSTDGVLKLSTPPAPDMSCIRSSLLPN
                     LCEAVAKNERYFNDFAIFEEAQVFFDKNYTNAYDETESLPEQRRHIGAAFASSVKDIG
                     MLFREAKGVLEYMPRYTHMEAYEFKKEEKPVWADNVVWLNIYLDGEKIGDMGLVAKKV
                     SMECGIKNLSVILFEMDASKLKPLKSRTNKFEHLAEYPETDYDISMLFDSDAVWNDIY
                     DAVMGKKKASALLKDASFVDEYRGKQIPQGKKSVTIRLTIGSDEKTLTSQEIESVAEQ
                     VMKKLGKKMGAELRTQ"
     gene            7265..8290
                     /gene="queA"
                     /locus_tag="ACZPD4_04220"
     CDS             7265..8290
                     /gene="queA"
                     /locus_tag="ACZPD4_04220"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1378309.1"
                     /translation="MKTSDFYYDLPEELIAQDPLEDRSSSRLMTVDKETGKIGHHVFS
                     EIIDYLNEGDCLVINDTKVIPARLIGAKEETGAKIEVLLLKRGEDDVWETLVKPGRKA
                     KPGTRISFGDGLLVGEVVDIVDEGNRLIHFEYEGIFEEILDQLGQMPLPPYITHQLQD
                     KNRYNTVYAEHSGSAAAPTAGLHFTPELLRKIEEKGVDIARVTLHVGLGTFRPVKVDD
                     VENHHMHSEFYMINEEAAEKINHAKDRGGRVICVGTTSCRTIESAADENGRLKACSGW
                     TEIFIYPGYRFKTLDCLITNFHLPESTLIMLVSALAGKEHVMAAYEEAVKERYRFFSF
                     GDAMLIL"
     gene            8549..8683
                     /locus_tag="ACZPD4_04225"
     CDS             8549..8683
                     /locus_tag="ACZPD4_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378310.1"
                     /translation="MYSEYIFDLSDKNVSDYVIYGKFTTSNMHIKGKWSVTFPLEKVR
                     "
BASE COUNT         2806 a         1498 c         2313 g         2270 t
ORIGIN      
        1 gtatcggatc ttattgcttt gcaggcgcct aattcgtatt atttaaaaga ttctatggaa
       61 gaagtaaagc agaatgtaac agacttcaat caaaatatta tagattatga agcgcagcat
      121 aagtcagaag caaacgggga attggcagat ctgcttcagt cattatttgc aacgcttaca
      181 gaatattata caaacggaac gaatgtgaca tcatatcagt cgggagactg tttcggaaat
      241 tcacatatgc cggagctttg tcagcatgta ctgacagcga atgagtattt gaaagaaaat
      301 gcggaagaga tcgagcttgc agaagtaaag atgcaggaag tattcgcaca gcaatatgaa
      361 gatgcgtgta aggcaagaga agaggaaggc gcaataaaat tgctgacagg aggcgctgcg
      421 gcaattaccg gaatcttggc aatcgtcgga actggaggga tggcaactcc ggttgtgata
      481 ggcgtatggg ttacaggagg atcgaccgct ctctatgggg cgagtaaagc gatagagggc
      541 attcaggacg ttcattatgg aagagctaaa gatctgacaa gtaagtcttt taattttatc
      601 agagatacag tatttcaggg gaatcaagag gcatatgacg catggggaaa tataaatatg
      661 atggttgcgg gtcttttagt tcctattggt caaggaacta aggcgggggc cggtcttgga
      721 gtaaaggcaa tcgcgcgcga aagcgcgaaa cgagtagcaa aagaggctgt aaaagacatg
      781 gcggttgatt ttactgccga aactatgaca aactgggcgc gtgaagagct ggatttgggc
      841 gactttcaga cggaacttct taaaacagga cttgagtttg gattggataa aggacttgat
      901 aaggtgggaa gtaaacatgt tccgtttaca gatgatatga cattttcaga ggcgaaacgg
      961 tataatcaat cgatgcagaa catggaaagc ggaaaacata accctccggg actatctgat
     1021 gcagatatgg atgcgtggga ttttgcaaat cagaaagtgt ccgagcatat agcggtgaag
     1081 aaagttgatc acaatgaagt gataaaaatc cgttcagagt atgcgaaaaa acaggaaagc
     1141 tttttgcatc caaagacaga tgcagacagc gcaaagactg cgacatttac aaatcaggaa
     1201 gcagaagatc tcgcatttgg cgcaataaaa ggaaagggta aatcggatgc cgtcgtgctt
     1261 ggtaagtttg aggacggaaa atcgacttca tatgataaaa tagcgcaaga atatgacgca
     1321 cagtattata atttggacga gtgggatgag ctggcaaaaa cgcattcaag agatgaaatg
     1381 tggaaagtaa atgagaaatt tcttgatatt gaaatcgcgt cgggaagaga tatatactta
     1441 tctcatgatc cggcgaaatt cagcggagac ggaagttttt ttgcgaaaga gatcgagtat
     1501 ttgcggcagc atggatataa atttgtaaaa gagggtgatt tgtggcatgc tgtacagtaa
     1561 ggctgagcgc aatttgactt atcaagacca gagtaaaggc ggattgcagc ttgaaaaata
     1621 aggaggctgt aaaacggtga gtatgaaaac gagagatgaa aaagaaaaga aacgagattt
     1681 atttacaaca attttttgga ttttagtgct ggtttttttg attgttaaaa ttgtaatccc
     1741 ggaaacaggt gtattgggaa tggtaaatga aggttcaaat gcggttttat ttttcggtat
     1801 cggaggaagc tgtgtgtgga cgatcattca taaaaggaag acaaataaaa cggcggttgt
     1861 tttcggagta ttgctgctgg tattgacatt aggtattggc ggatggatat caggaaagct
     1921 tgtattggat cttatatcag gaccggaggt tgtgcgtctg acgtcagtta ctatgcgtga
     1981 atcgtcggga atatatggtc ttatttcacg gcattactat ttatgcggaa gagatgacga
     2041 gtggacggaa tatcagatgg aaatttcttc ggatgagtat tatgagttag aaggagaaga
     2101 gcaggtgact gtgttgtgtt acccaagaac agagcggatt ttaaagtttt tgtgattttg
     2161 ccgacagaca tcatgtatcg gattgaaaca ggaagcccac aggcttcaga caatgggtag
     2221 ttcacaatat gaatatatgg agcggaaacg tatggagaca gagcaaaagg aaatgatgtg
     2281 taaatataag aagattattt gtaaaatatt cggcgaacag atacgggtga tcggtgaaag
     2341 cagtgcgatc ggccctatgg ggcagtttca gatcagattt ttctatgagc cgacaaaaat
     2401 ttatgttaca ctggatgcag accgcggggc atttacgttt gatctgaaag atgaagcgaa
     2461 agattggaat acgttgtaca gaataaaaaa gtttgacaac tgtatgacag aaaagtgtct
     2521 tgaaaatgcg gcggttattt tgaagcaggt tcttgaggaa aataagttcc ctctgtataa
     2581 atcggagaat gataagctgt acaaaaagca ggatggaacg taccgcagga taaaagatat
     2641 ctatgcagaa ctggcaggag gagagtaaat ttatggctat aaaagaagtg tcagaacgat
     2701 atttggaatt aaggcaaaat gctttggatt atacatttga acagatgaat cttcagcttg
     2761 aaaatgataa gcaggtttat ctagcagtat ttgatatacc ggtggagagc gcgatcatcg
     2821 gtaataaaac gaagactctt gtgctcgtgt ttggattgaa catccatatt tattgtgcaa
     2881 atggtgatgc cgtgaccgga ttggagcaga atgcgaaagc gaaacaggcg atgcagtctc
     2941 ttttcatcag ttgtccgcaa gcgcttgatg agatgacgct gactcacaag acagattttt
     3001 acgaaagtaa aaatgtaaga gcttatttaa aaacaagaaa aggcgtttac ttcaaagagc
     3061 ttacaggaga aactaagaaa gaacgctttt tggaaatgct tatgcggaac gtgacagagg
     3121 aggtaaattt ccgtcattga caaaaaacgt aatctgtgcc aaaataattt agaatcagcc
     3181 gattggaaca atgtttgttg ttacaaagcg ggagattaaa aaatataaca gaaacaaaat
     3241 cataatttca gaaattagac tatgaacata tgtgagtaga gtaggcagag cgacccagag
     3301 acggagtgtc atcggctgag agcattccgc gcgacccttc ttgaagtttc agtatgggag
     3361 tcttccggcg aaaagcagtt tgtgcgttaa gtcggatgtt cgtgtagcaa tattttacac
     3421 aatgagagcg cagagcagat aggatgctct gaaatagggt ggtaccgcga ttaacatcgt
     3481 cccttgttga acagacaagc gacggtgttt ttttgtatag gcgacgggcc aaagtccggg
     3541 cttgcccgag accttggcga ccgcttacaa tcccggaatg tgccgtttgg cacttccggt
     3601 gattatgcta tagaaaaatt cgatttccta tggcatagat cccttaggga ggatgcgcac
     3661 gccgcatcga ggaagttcaa atgaaaaaac aatttgggaa ggagaaataa aatgaaagat
     3721 aaaatcaaca gtattttatc agaagcaaaa ggtaagattt ctgatgcagt gagcgagggt
     3781 gaactgcaaa atgtaaaaag cgcgtatatc ggaaagcagg gttcattaag tcttttgatg
     3841 aaagagatcc ctacagtgga tgtggcgctt cgtcctgaga tgggaaaact tttgaatcag
     3901 gcgaaaaatg aagtgaagga cctgatcgat gagaagagga tggagctgaa gctgaaagct
     3961 tcggaagtgt ctcctgattt tgactgttct gttccgggta tcctgccgac aggcggcgga
     4021 cttcacccga tcacgcagat gtgttatgac ttgaacgata cgttccgttc gatgggattc
     4081 gaggtgtttg aggaagacga gatcacaagc gagatgtatg cgtttgataa attgaatttc
     4141 ccgccgaatc atccggcgcg tgagagtatg gacacatact ggctggaagg acatgacagc
     4201 ggaagcacga atgagaagct ttgccttcgt ccgcatctga caggcggaag tgttcgctat
     4261 atgcagacac acaaaccgcc gtatcgtttc gtatatccgg gtcgcgtgta cagaaatgag
     4321 acaacggacg cgcatcatga gagagcgttt ttccagtatg aggcgctgat cgtggacaaa
     4381 gatattacgt ttacatcagg taaagtaatg attaagagta ttttgagcaa agtgtttgga
     4441 acggacgttc cggtcagaat gcgttccggt ttcttcccgt ttgtagagcc gggatttgag
     4501 atcgacatgc agtgtcaggt ttgcggcgga aaaggatgca gcgtatgtaa acatgtcggc
     4561 tggatcgaag ttatgccggg cggttctccg catccgaacg ttcttagggc agcagggctt
     4621 gatccggatg agtatacagg attttatgta aatatcggtt tggacagact tgttatgatg
     4681 cgctacggcg tggatgacgt aagattgttc cacagcgcag atttaagatt cctgaaacag
     4741 tttagataga ggcaatttag atacgaggag agagactatg aaattatcat tatcatggat
     4801 tagagattat gtaaatattc cggaagatgc agatctgaaa aaactggcgt acgatctgac
     4861 aatgtctaca gttgaagtag aagatgtgga atatcttgca gaacgttttg aaaatatgat
     4921 cgtcggagtg atcgaaaaga tcgagccgca tccgaatgca gacagactgc gagtatgtaa
     4981 agtaaatatc ggcagtgatg agatcaaaga catcgtatgc ggaggcatta atctggaaga
     5041 aggaatgcgc gttgcagtat cttgtccggg agcggttgtc cgctggcacg gcgagggtga
     5101 gcctgtagtg atcaaaaatt ctaaacttcg cggagttgag tcattcggta tgatctgcgc
     5161 ttcagatgag atcggattag gcgagctgtt cccgtcagaa acagaaggag aaatccttga
     5221 tctgtcagcg tttgacgttc cggcaggaac ttctttggca gatgctttgg atatgaatga
     5281 cgtgcttctt gagatcgaca ataaatcaat gacaaacaga ccggaccttt ggggacatta
     5341 cggaatcgcc cgcgagatcg cggctcttta taacttgccg cttgcaaaga tcgagccttt
     5401 ccgggcagac gttcagtctg attttaaagt tgagatagaa gatcctgaca gatgtacaag
     5461 atatatcggc gttgaaatgt caggcgtggc tgtaaaacct gcgccgtata aaatgcagaa
     5521 tagaatctgg aaagtaggaa tgcgtccgat caatgcgctt gttgatatca cgaactatgt
     5581 aatgcttgca acaggaaatc cgacacatgc atttgacgcg gacaatatta cggatcacat
     5641 tgttgtaaga catgcaggag aaggtgagaa actgcttctt ttaaatgata aagaacttga
     5701 attaaacgca gatgatcttg taatcacaga ttccgaaggc gcggttgctc ttgcaggagt
     5761 tatgggcgga gcgaaagatt cgatcctgcc gaagacggaa cgcgtgattc ttgaagtggc
     5821 aaactttgaa gcgacaggca tccgccgtac ggcgcttcgt tacgatacgc gtacagaagc
     5881 gtcttccaga tatgagaaag caatcgatcc tgaaagatgc gatcaggcgc ttgctctttc
     5941 aatgaagtat tttaaagagc tttatcctga acttcaggtg acaggatact gcgataatta
     6001 tgtgaaaaaa ctcgacagag cagagattga cgttaatctg acatggcttg agaagagact
     6061 tggaaaacat ttgacaaacg aagaaatcca gggcaaatta gagcagcttg gattcgatgt
     6121 tcagatcgac ggagacaaca tgcatgtgac agcgccgaca tggcgttcga caggagatat
     6181 ttccattaaa gacgatgtga tggaagaagt tgccagaatg tacggatacg ataatttcga
     6241 ggcgacagag tttacgacga catttacaga ctcgatcaat cagaaagata agagtcttgt
     6301 aagaaatatt aaagaatatc ttgcaatacg ctgtggaatg caggaagtat acacttatcc
     6361 gtggatgaac gatgtgtttg taaacgcagt tctgcaaagc acggacggcg ttttaaaact
     6421 ttctacacct ccggcgccgg atatgagctg cattcgttct tctcttcttc cgaacctttg
     6481 cgaggcggtt gcgaaaaacg agcgttactt taatgacttt gccatttttg aagaagctca
     6541 ggtattcttt gataaaaatt atacaaatgc atatgacgag acagaatctc ttcctgaaca
     6601 gcgcagacat atcggagcgg catttgcaag cagcgtaaag gacatcggta tgcttttcag
     6661 agaggcaaaa ggagttcttg agtatatgcc gagatatacg catatggaag cgtatgagtt
     6721 taaaaaagaa gaaaagccgg tatgggctga caatgttgtg tggctgaata tttaccttga
     6781 tggcgagaaa atcggcgata tggggcttgt agcgaaaaaa gtttctatgg aatgcggcat
     6841 taagaatctt tctgtgattc tttttgaaat ggatgcttca aaactgaaac cgctgaaatc
     6901 aagaacgaat aaattcgagc atcttgcaga gtatcctgag acagactatg acatttccat
     6961 gctttttgat tccgatgcag tatggaatga catttatgat gccgttatgg gtaaaaagaa
     7021 agcaagcgcc cttttaaaag acgcatcgtt tgtagatgaa tacagaggaa aacagattcc
     7081 gcagggcaaa aaatccgtaa cgatcagact gacgatcgga tctgatgaga aaacgctgac
     7141 atcacaggag atcgaaagcg tcgccgaaca ggttatgaaa aaactcggaa agaaaatggg
     7201 cgcagagctt agaacacaat aaaacaaggc ttgcttctta gaaataacag gagataaacc
     7261 gaatatgaaa acaagtgatt tttattatga tcttccggag gagttgatcg cgcaggatcc
     7321 gcttgaagac cgttcctctt ccagacttat gacagtggac aaagaaaccg gtaagatcgg
     7381 acatcatgta ttcagtgaga ttatcgatta tttgaatgaa ggcgattgtc tcgtgataaa
     7441 tgatacaaag gtaatccctg ccagattgat cggagcaaaa gaagaaaccg gagcgaagat
     7501 cgaagtgctt cttctgaaaa gaggagagga tgatgtgtgg gaaacgctcg tgaaacccgg
     7561 tcgtaaggca aaaccgggaa cgcggattag tttcggagac gggctgctcg taggagaagt
     7621 tgtcgatatc gtggacgaag gaaacagact gatccatttt gaatacgaag gtatctttga
     7681 agaaatatta gatcagctcg gtcaaatgcc tcttccgccg tatattacgc atcagcttca
     7741 ggataagaac agatataata cggtttatgc agaacattca ggttcagcag ccgctccgac
     7801 ggcaggactt cattttacgc cggaactact cagaaaaatt gaagaaaaag gcgttgatat
     7861 tgcaagagta acattacatg taggtttggg aacatttcgt cctgtcaaag ttgacgatgt
     7921 ggaaaatcat catatgcact cggaatttta tatgataaat gaagaagcgg cggagaagat
     7981 caaccatgcc aaagacaggg gcggacgcgt gatctgcgtc ggaacgacaa gctgccgcac
     8041 gattgaatct gcggcagatg aaaacggacg tttaaaggct tgcagtggct ggacggagat
     8101 ttttatttat ccgggttatc gttttaaaac gctggactgc ctgatcacaa acttccattt
     8161 gccggagtca actcttatta tgctcgtatc cgcgctggca ggaaaagaac atgttatggc
     8221 ggcttatgaa gaagcggtga aagaacgata tcgtttcttt agctttggag atgccatgct
     8281 gattttgtaa agtaattcac gacaaaataa tcggcaaccg gcctatgggg ttaatcctcc
     8341 ccatgcctaa aaagtaggaa tagaaaaacc cacgaggtgt gcactcgtgg gttttttgtt
     8401 gacgctcaac atggatacgc cgtttctttt tgcctgctgg cattatagca tatgcaaata
     8461 ttgaatgcaa tattcatttt ccttgagttt ccgcagtctt gattgtgaag caagtatttc
     8521 tttctttaca gatgaaggag aaaaagatat gtacagcgaa tatatctttg acttgtcaga
     8581 taaaaatgtt tcggattatg ttatttatgg aaagttcaca acaagtaata tgcacattaa
     8641 agggaagtgg agtgttacat ttccattgga aaaggtaagg taataaaaag gtaagataat
     8701 aaacagcgaa aacccgctaa ccttccggct aacgggtttc gttgttttat agttatctaa
     8761 aggaatgttt acgcatttac aaagatatgt aggtttgtca aacatatgtt acaccccgat
     8821 caataaattc cctcagaaca gtttgtggtg atttccaccc cagaggtctc atgggaaact
     8881 ggttata
//
LOCUS       DBMDXF010000070         8869 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2127_length_8869_cov_1.806683, whole genome shotgun sequence.
ACCESSION   DBMDXF010000070 DBMDXF010000000
VERSION     DBMDXF010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2127_length_8869_cov_1.806683"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /gene="htpG"
                     /locus_tag="ACZPD4_04230"
     CDS             complement(<1..1963)
                     /gene="htpG"
                     /locus_tag="ACZPD4_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1378311.1"
                     /translation="MAVKKGTLSIDSENIFPIIKKWVYSDHDIFVRELISNGCDAVTK
                     LKKLDMMGEYELPEDYKAKIEVIVNPEEKTMKFIDNGLGMTAEEVEEYITQIAFSGAT
                     QFLEKYKDKTTEDDMIGHFGLGFYSAFMVADEVQIDTLSYKEGASAVHWASQGGTEYE
                     MQEGNKETVGTEITLFLNEDSLAFANEYRAREVIEKYCSFMPVEIFLSKANAQPEYET
                     IDEEDVLDTDEVVEHITEEVKEGEEGEPKKKAKIVKRPVSLSDTHPLWSKSPSECTKE
                     EYIDFYRKVFMDYKEPLFWIHLNMDYPFNLKGILYFPKINTEYESIEGTIKLYNNQVF
                     IADNIKEVIPEFLMVLKGVIDCPDLPLNVSRSALQNDGFVNKVADYISKKVADKLTGM
                     FKTDRENYEKYWDDISPFIKFGCLKDEKFGEKMKDSMIYKNLDHKYLTLEDIINESKA
                     AGTEEETAEEAAAETDVQTDTDDQDKEPEKTSVYYVTDEVQQSQYINMFKAQGQDAIV
                     LTHNIDSAFVTYLEQKHEDVRFLRIDADVHESLKDEVAEDEKESFGKMADSLVEIFRK
                     ELNDDKLDVKVEKLKDDKVASMAVLSEENRRMEEMMKMYGMSGMDSAMFGSKATLVLN
                     ANHPLVKFLTENKESENVTVICKQLYDLAM"
     gene            2214..2744
                     /locus_tag="ACZPD4_04235"
     CDS             2214..2744
                     /locus_tag="ACZPD4_04235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1378312.1"
                     /translation="MDQTSSDNRTDQPFTIRKAETNEDLQEIAALADVIWHEHFTPII
                     GKEQVEYMLSRFQSYPALQDQVLTGYEYYRLLGNRKLCGYMAIRQDQDGRMFLSKLYI
                     AKQYRGRHLAAFLFDFLKNLCRQRNCSAIWLTCNKHNDHSLNVYRHFGFQTIDAQKND
                     IGNGFFMDDYIMEYRL"
     gene            complement(2844..3362)
                     /locus_tag="ACZPD4_04240"
     CDS             complement(2844..3362)
                     /locus_tag="ACZPD4_04240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1378313.1"
                     /translation="MANPIVTFEMENGDIMKAELYPEIAPNTVNNFISLINKGFYDGL
                     IFHRVIRGFMIQGGCPNGNGMGGPGYAIKGEFAQNGFQNDLAHDPGVLSMARAMHPDS
                     AGSQFFIMHEKSPHLDGAYAAFGKITEGMDIVNKIAETATDYSDRPLEEQRMKKVTVE
                     LFGETYDEPETV"
     gene            3562..4674
                     /locus_tag="ACZPD4_04245"
     CDS             3562..4674
                     /locus_tag="ACZPD4_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378314.1"
                     /translation="MASLSLKGVQKIYPNGFHAVKDFNLEIADKEFIIFVGPSGCGKS
                     TTLRMIAGLEDISDGVFKIDGKVMNDVEPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     LRKVPKAEIDKKVREAARILDLDKLLDRKPKALSGGQRQRVAMGRAIVRNPKVFLMDE
                     PLSNLDAKLRVQMRIEISKLHENLGATIIYVTHDQTEAMTLGTRIVVMKDGVVQQVDT
                     PQNLYNTPCNLFVAGFIGSPQMNFMDAVCSVKGDDVTLTVGKHVLKVPASKKQALING
                     NYDGKTVVLGIRPEDVHDSQAFISNSPDSIIESEIKVYELLGAEVFLYFDVEGTQMTA
                     RVNPRTTLRTGDKAVFALDMEKIHVFDKETELTITN"
     gene            4823..5767
                     /locus_tag="ACZPD4_04250"
     CDS             4823..5767
                     /locus_tag="ACZPD4_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1378315.1"
                     /translation="MGENTEQTTNRKTETTAQMNELNKALLELKKITGISLEVSGAAP
                     EQIEPALTQIHCLCTAYKEKYNRTDFLQGLMTGNIPHYEISDRASRLHIDPEEKRILF
                     LIEGKHFDETSGAILKNLLSCRSKTYIVPLTETLLAVIRPLCQNETFEDMRHIAETIV
                     DTLNTEALIRVRLSYSSVMNTLADIQKAFQETNLALKVGRLFYSEQNIFPYNELGIGR
                     LIYRLPVSLCENFLSEIFGSPIPKTLDPELMATVNRFFLNNLNIAETARQLHMHRNTL
                     IYRLEQIEKQTGLDLRLFDDAMTFKIAMMVINYLKGNK"
     gene            5770..6270
                     /locus_tag="ACZPD4_04255"
     CDS             5770..6270
                     /locus_tag="ACZPD4_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HIR1378316.1"
                     /translation="MNDALYEQLVPRKQKTSGILISILIIVLAVAVAVLGMPLVGFLS
                     FLIAVLILMIAFYFIFPKLNVEYEYMILNHDLQIDAIYNKSKRKHMLSFDIQSAEIIA
                     PKKSPRLNAFHPDKTYDFTSGNENADVYAIMISLDQKNSCVYIEPDRKMIEHMRQWMG
                     SKMFLD"
     gene            complement(6388..7065)
                     /locus_tag="ACZPD4_04260"
     CDS             complement(6388..7065)
                     /locus_tag="ACZPD4_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR1378317.1"
                     /translation="MKYEIKGGELPVVVCFMDAGETMITEKGSMSWMSPNMKMETTSN
                     GGVGKALGRMFAGEAIFQNRYTAEGGPGMIAFASSFPGQIRPWEIAPGKEIVVQKAGF
                     LAAQESVELSIFFQKKLGSGLFGGEGFIMQKISGHGLAFLEFDGSVIEYVLQPGQQIV
                     VDTGYLAAMEATCNMEIRTVPGIKNMMFGGEGLFNTVITGPGRVWLQTMPISNVAGAI
                     MKVFPAR"
     gene            complement(7215..>8869)
                     /locus_tag="ACZPD4_04265"
     CDS             complement(7215..>8869)
                     /locus_tag="ACZPD4_04265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378318.1"
                     /translation="GQISSITTLTDVTFIYGITEENLEAYGNIIASENGEYLEYYNIS
                     YTTAFRDTLEMLSVLLIAAAILMPFVKCLDLSEMKVFRASFEVAGAGALFFAFGGFDI
                     MAYVVYHTVKGRSLPAGFEPALLVANFLLWMFMFGILFWCVTSLRAMFRMKKAYWTER
                     TLTAKMLRKMKNKGEESGGKIQKKVKSIGRKIRNFFARQYDALLHLDFQDKTNRTILR
                     VVLINFAILFVVCLFWWYGTVALIVYSVMLFMFLKKYTQDLSEKYALLLKSTNQLAEG
                     HLDIPIEGDVGLFNPIQEELKRIQKGFKKAVDEEVKNERMKTELVTNVSHDLRTPLTA
                     IITYTDLLKNEKDEEKRKEYISVLERKSLRLKVLIEDLFEISKAASKSVVMHFMKVDI
                     VGLFKQVELENVEKIKAANLEFRTKIPEQKVVMWLDSEKTYRIFENLIVNITKYAMPH
                     TRVYIDMTETEDGVHITMKNVSAAELNFNADEITDRFVRGDSARNTEGSGLGLAIAKS
                     FAELQHGSLKISTEADLFKADLYLPKSKEMPEKPGGGYPENL"
BASE COUNT         2434 a         2123 c         1588 g         2724 t
ORIGIN      
        1 gcattgcaag atcataaagc tgtttacaga tgacagttac attctcgctt tctttgttct
       61 ctgtcaggaa cttcacaagc ggatggtttg cgttgagtac aagtgtcgcc ttgctgccga
      121 acatagccga atccattccg ctcataccgt acatcttcat catctcttcc atacggcggt
      181 tttcttctga caaaacagcc atagacgcca ctttatcgtc tttaagcttt tccactttca
      241 catcgagctt atcgtcattt agttcttttc ggaagatttc cacgagactg tctgccattt
      301 ttccgaaaga ttctttctcg tcttctgcaa cttcatcttt caaagactca tgtacatcgg
      361 catcgattct taaaaatctg acgtcttcat gtttctgttc cagataagtc acaaacgcag
      421 aatcaatatt atgtgtcagt acgatcgcgt cttgtccctg tgccttgaac atattgatgt
      481 attggctctg ctgaacttcg tctgtcacat agtaaaccga cgttttctca ggctctttat
      541 cctgatcgtc cgtatctgtc tgcacatctg tttccgctgc cgcctcttcg gctgtctctt
      601 cttctgttcc tgcagcttta ctttcattta taatatcttc taatgtcaga tatttgtgat
      661 ccaggttttt gtagatcatc gaatctttca tcttctcgcc gaatttttca tctttcagac
      721 atccgaattt gataaacgga ctgatgtcat cccagtattt ctcataattc tctctgtctg
      781 tcttaaacat tcccgtcagc ttatccgcca ctttcttaga aatgtaatcg gcaactttat
      841 tgacgaatcc atcgttttgc agggcgcttc ttgatacatt aagcggcaga tccggacagt
      901 cgatcacacc tttgagaacc ataaggaact ccggaatcac ttccttgata ttgtccgcga
      961 taaaaacttg attgttgtac agcttgatcg ttccttcgat agattcatat tccgtattga
     1021 tcttcggaaa atacaaaatt ccttttaagt taaacgggta atccatattc aaatggatcc
     1081 agaacaaagg ctctttataa tccatgaaca ctttgcggta aaaatcaatg tattcttcct
     1141 ttgtgcactc gctcggactc ttgctccaca gcggatgagt atcgctcaaa gaaacagggc
     1201 gtttcacgat cttcgctttc tttttcggtt ctccttcttc gccttctttt acttcttccg
     1261 taatatgctc tacgacttcg tctgtgtcaa gcacatcctc ctcatcgatc gtttcatact
     1321 ccggctgcgc atttgctttg gaaaggaaga tttcaacagg cataaaagaa caatattttt
     1381 caatcacttc tcttgcgcgg tattcatttg caaacgcaag actgtcttca tttaaaaaca
     1441 gtgtgatctc tgttccgacc gtctctttat ttccttcctg catctcatat tccgtacctc
     1501 cctgacttgc ccagtgtacg gcagatgcgc cttctttata agacaatgtg tcgatctgaa
     1561 cttcatccgc caccataaac gcagaataaa atccaagtcc gaaatgaccg atcatatcgt
     1621 cttctgtcgt tttatcttta tatttttcaa gaaactgtgt cgctcccgaa aatgcgatct
     1681 gcgtaatgta ctcttctacc tcttcggctg tcataccaag accgttatcg ataaatttca
     1741 ttgtcttttc ttccggattg acaattactt cgatctttgc cttataatct tcgggaagtt
     1801 catattctcc catcatatca agctttttca gcttcgtaac cgcatcgcag ccatttgata
     1861 taagttccct cacaaaaata tcatgatccg aatacaccca tttctttata atcggaaata
     1921 tattttcgct gtcgatcgat aatgttcctt ttttcactgc cattgtaaac actccttatc
     1981 tttttttgat tttatgactt acgcatctcc cggtatactt tatatacatc ggcttcatgc
     2041 gatctgtttt ttatgtctaa cagacattct agtacggcaa tatttaaaag tcaatagaaa
     2101 atcagcactc ttttgtgttg agtgctaata attaatatta tttttagaaa gttgcggaaa
     2161 atatgttatt tgtactataa tatttgataa aaaaagaagg gggaatattt ctcatggatc
     2221 agacatcttc agacaatcga acggatcaac cgtttacaat ccgcaaggca gaaactaacg
     2281 aggatcttca agagatcgcc gctttggcag atgtgatctg gcatgaacat ttcacgccga
     2341 tcatcggcaa agagcaggta gaatatatgc tgtcacgttt tcagtcttat ccggctttac
     2401 aagatcaggt tcttaccgga tacgaatact accggcttct cggcaaccga aaactgtgcg
     2461 gatatatggc gatcaggcag gatcaggacg gaaggatgtt tttgagcaaa ctgtacattg
     2521 ccaaacaata ccgcggcaga catttagccg catttttgtt tgattttcta aaaaatttat
     2581 gtcggcagcg aaattgttcc gccatttggc tgacatgtaa taagcacaac gatcactctc
     2641 tgaacgtata ccgtcacttt ggttttcaga cgatcgacgc tcagaaaaat gatatcggaa
     2701 acggcttttt catggatgat tacattatgg aatatcggct gtaaatactg tgacataaca
     2761 atccccttaa acaggttttc gtttccttgc ctgcctaagg ggaatatgtc agcatcttta
     2821 cttttctttt ttgcctttac agcttatact gtctccggtt cgtcatatgt ttctccgaac
     2881 aattctacag tcaccttttt cattctctgc tcttcaagcg gacgatcgct gtaatctgtt
     2941 gcggtttctg cgatcttatt tacaatatcc attccttccg tgatctttcc aaacgccgca
     3001 taagcgccgt ccagatgcgg tgatttttca tgcatgataa aaaactggct tcccgcagaa
     3061 tcaggatgca ttgcacgagc catggaaaga actcccggat catgtgcaag atcgttttgg
     3121 aaaccgttct gtgcaaactc gcctttgatc gcgtatcccg gaccacccat tccgtttccg
     3181 ttcggacatc cgccctggat cataaatccg cggatcacac ggtgaaagat cagaccgtcg
     3241 taaaatcctt tattaatcaa tgaaataaag ttatttaccg tattcggcgc aatctcggga
     3301 taaagctccg ctttcatgat atcgccgttt tccatctcaa atgtaacgat tggatttgcc
     3361 ataattttat tcctcttttc tttactgttt tcatcgctta taacagtgct attgtatctg
     3421 atttctcatt tttcgtcaac atgtacactt tccgccgctt tttttagtca ctcatgacat
     3481 tgttctgttt ctggcttacg gttatactta tctcataaga aaaatccaga aaaacaagtt
     3541 tatttttagg aggaacataa aatggcaagt ttatcactta aaggcgttca gaaaatttat
     3601 ccgaacggat tccatgcagt aaaagatttc aacctcgaaa tcgcagataa agaattcatc
     3661 atcttcgtcg gtccgtccgg atgcggtaaa tctacaacac ttcgtatgat cgcaggtttg
     3721 gaagatattt ccgacggagt attcaaaatc gacggaaaag ttatgaacga cgtagaaccg
     3781 aaagaccgtg atatcgcaat ggtattccag aactacgcat tgtatccgca catgacagta
     3841 tatgataata tggcgtttgg tctgaaactt cgtaaagtac cgaaagcaga aatcgacaaa
     3901 aaagtccgtg aagcggcaag aatccttgac cttgacaaac tgctcgaccg gaagccgaag
     3961 gctctttcgg gaggacaaag acagcgtgtt gccatgggac gtgccattgt tcgtaatccg
     4021 aaagtattcc ttatggacga acctctttcc aaccttgacg caaaactgag agtccagatg
     4081 cgtatcgaga tttcaaaact tcacgaaaac ctcggcgcaa cgatcatcta tgtaacacat
     4141 gaccagacag aagccatgac tctcggaacg agaatcgtcg ttatgaaaga cggagtcgtt
     4201 cagcaggtcg atacaccgca gaacctttac aatacaccgt gcaacctgtt cgttgcagga
     4261 tttatcggat ctcctcagat gaactttatg gatgccgtat gctctgtaaa aggtgatgat
     4321 gtaacattga ctgtaggaaa acatgtatta aaagttcctg cttccaagaa acaggctctt
     4381 atcaacggaa actatgacgg aaaaacagtt gttctcggca tccgtcctga agacgtacat
     4441 gattctcagg catttatcag caattcaccg gacagcatta ttgaatctga gatcaaagta
     4501 tacgaattgc tcggcgctga agtattcctt tactttgacg tggaaggaac acagatgaca
     4561 gctcgtgtaa atcctcgtac gacactgcgt acaggcgata aagccgtatt cgcgcttgat
     4621 atggaaaaaa ttcacgtatt tgacaaagaa accgagttga cgattacaaa ctaatgattt
     4681 aaaatcaaaa cagcagttac atagtgtcag aacgcccggt ttgatcagga tctcatttct
     4741 gacaatactg cttataataa gaatttaaag ggggctgcac ttattacagg tgcggcctct
     4801 ttattcaaaa cgaggttaat ctatgggaga aaatactgaa cagactacaa accgcaaaac
     4861 agaaactaca gcacagatga atgaactaaa caaagctctg cttgaattaa aaaaaattac
     4921 cggaatctcg cttgaggtat ccggtgcagc gcctgaacag atcgaacctg cgctgacaca
     4981 gatccactgt ctttgcacag cttacaaaga aaaatataac cgcactgatt ttctgcaggg
     5041 actgatgacc gggaatatcc cgcactatga aatttccgat cgggcatcga gacttcacat
     5101 tgatccggag gaaaaacgaa ttcttttcct tatagaagga aaacactttg atgaaacttc
     5161 aggcgcaata ttgaaaaatc tactgtcctg ccgttcaaaa acttatatcg ttccccttac
     5221 ggaaacgctg cttgcagtca tacgtccgct ttgccaaaac gagacgtttg aagatatgag
     5281 acatattgcg gagacgatcg ttgatacgct caataccgag gcgcttatcc gtgtccgcct
     5341 ctcatacagt tctgtaatga acacgcttgc cgatattcaa aaagcttttc aggaaacgaa
     5401 tcttgcgttg aaagtcggaa ggctctttta ttcagaacaa aatattttcc cgtataacga
     5461 actcgggatc ggcagactga tctatcgtct tcctgtttca ctgtgcgaga actttctgtc
     5521 tgaaatattc ggttctccta taccgaaaac gcttgacccc gaacttatgg caacagtcaa
     5581 ccgtttcttt ttaaacaact taaacattgc cgaaaccgcc agacaactcc atatgcacag
     5641 aaacactctt atctaccgtc tggaacagat tgaaaaacag acaggtctgg atcttcgtct
     5701 ctttgacgac gccatgacat ttaagatcgc tatgatggtc ataaattact tgaaaggaaa
     5761 caaataacta tgaatgacgc attatatgaa caactcgtac cccgcaaaca aaaaacttcc
     5821 ggcattctca tcagcatact tatcatcgtt cttgcagtcg ctgttgctgt tttaggaatg
     5881 cctctggtcg gttttctgtc atttcttatc gcagttttga tcctgatgat cgctttttat
     5941 tttatctttc caaagctcaa tgtggaatat gaatatatga tcttaaacca tgatcttcag
     6001 atcgatgcca tttacaacaa gtccaaaaga aaacatatgc tcagttttga tattcagtct
     6061 gccgagatca ttgctccgaa aaaatctccg cggctgaacg ctttccatcc tgacaaaaca
     6121 tatgatttca cttcaggaaa tgaaaatgcc gacgtctatg ccattatgat ttctctcgat
     6181 cagaaaaact catgcgtata tatcgaacct gacaggaaaa tgatcgaaca tatgagacaa
     6241 tggatggggt caaagatgtt tttagattaa acgtgacgtg caatacgatc tgcaaaaaaa
     6301 ttcttctttt ttagcagtct ggcaggattg gtatcaatat ccgatcctgc cagactgctg
     6361 tttttattat atagcatgct attatattta tcttgccggg aaaactttca tgatagcgcc
     6421 tgccacattt gaaatcggca ttgtctgaag ccatacccgt cccggtcctg taattaccgt
     6481 attaaacaga ccttcgcctc cgaacatcat gtttttgatt ccgggcactg tgcgaatctc
     6541 catattgcat gtcgcctcca tagcggcaag gtaacctgta tcgactacga tctgttgtcc
     6601 cggctggagt acatactcta tgacagatcc gtcaaattca agaaacgcca gaccatgtcc
     6661 cgaaattttc tgcatgataa atccttctcc gccgaacaga ccggaaccta attttttctg
     6721 gaaaaagatc gataattcaa cgctttcctg cgccgccaga aaacctgctt tttgcacaac
     6781 tatctctttt cccggagcga tctcccaagg tctgatctgt cccggaaaac tggatgcaaa
     6841 cgcgatcatc cccggaccgc cttccgctgt ataacggttc tggaaaatcg cctctcctgc
     6901 gaacattctt ccgagcgctt ttccgacccc gccgtttgac gtcgtttcca tcttcatgtt
     6961 cggtgacatc cagctcatag agcctttttc tgtaatcatc gtttctcctg cgtccataaa
     7021 gcagactact accggaagtt ctccgccttt aatctcatat ttcatcttct tttcctccct
     7081 tttcccattc tttatgccag catatttttc ttattctgtc tctctttata ccgactctgt
     7141 tttctatcca cagattatca taaaataccg gaagagtcac tactctcccg gcattattta
     7201 catatccgtt acatttataa attttcagga tacccccccc cgggtttttc gggcatttcc
     7261 tttgatttcg gtaaatacaa gtctgcttta aagagatctg cctctgtcga aatcttcaaa
     7321 gaaccgtgct gcaattcggc aaagctcttt gcaatcgcca gaccaagtcc cgatccttcg
     7381 gtgtttcttg ccgaatctcc gcggacaaac cgatccgtaa tctcatctgc gttaaagttc
     7441 aattctgccg cagaaacatt cttcattgta atatgcactc cgtcttccgt ctctgtcata
     7501 tcaatataaa ctctcgtatg aggcattgca tacttcgtga tattcacgat aagattctca
     7561 aaaatccgat atgttttctc actgtcaagc cacatgacaa ctttttgttc cggaatcttt
     7621 gttctgaatt ccaaattcgc cgctttgatt ttttcaacat tttccagctc aacctgctta
     7681 aataatccta cgatatcaac tttcataaaa tgcataacga cacttttgct tgccgccttg
     7741 ctgatctcga ataaatcttc gatcaacact ttcaaacgaa gagactttct ttccagaacg
     7801 ctgatatatt cttttctctt ttcttcgtct ttctcatttt tcaacagatc tgtatatgtg
     7861 atgatcgctg tcagcggcgt cctaagatca tgggagacgt tcgtgacgag ttctgtcttc
     7921 atccgctcgt ttttcacttc ttcatctact gcttttttaa atcctttttg aatccgcttt
     7981 aattcttcct ggatcggatt gaacagaccg acatcgcctt cgatcggaat atccaaatga
     8041 ccttctgcaa gctgattcgt tgacttgagc aaaagagcat atttttcaga aagatcctgc
     8101 gtatattttt tcaaaaacat aaacagcatc accgaataaa cgatcaacgc gaccgtaccg
     8161 taccaccaaa aaagacatac gacaaacaga atcgcaaagt tgatcagcac tacgcgaagg
     8221 atcgtccggt tcgttttatc ctgaaaatca agatgaagca aagcgtcata ctgccgggca
     8281 aaaaagtttc tgatctttct cccgatactt ttcactttct tttgtatctt tcctccgctt
     8341 tcttctcctt tattcttcat ctttcttaac atctttgccg tcagcgtcct ttccgtccaa
     8401 tacgctttct tcatgcggaa cattgcccga agcgatgtaa cgcaccagaa aagtatcccg
     8461 aacataaaca tccaaagcag gaaattcgcc acaagcaaag ccggctcgaa tcctgccgga
     8521 agagatcttc ctttcaccgt atgataaaca acataagcca ttatatcaaa accgccgaaa
     8581 gcgaaaaata aagctcccgc acctgccact tcaaaggatg cacggaatac tttcatctcg
     8641 cttaagtcga gacattttac aaacggcata agaatcgccg ccgcgatcag cagcacgctc
     8701 aacatttcaa gcgtatctct gaaagccgtt gtatagctga tattgtaata ctcgagatat
     8761 tctccgtttt ccgaagcaat gatattccca taagcttcca gattttcttc tgttatcccg
     8821 taaatgaacg taacatccgt aagcgttgta atgcttgata tctgcccgt
//
LOCUS       DBMDXF010000071         8823 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2136_length_8823_cov_1.992548, whole genome shotgun sequence.
ACCESSION   DBMDXF010000071 DBMDXF010000000
VERSION     DBMDXF010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2136_length_8823_cov_1.992548"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="ACZPD4_04270"
     CDS             complement(<1..1206)
                     /locus_tag="ACZPD4_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase domain-containing protein"
                     /protein_id="HIR1378319.1"
                     /translation="MDRNERNTMDQNARKRTVRREGNAEGSARANEGLGRRPSGNGAP
                     ASAGRPSGKSSSASVRRTSESGDPTRRTTPGGTVTPMRRESGSVRKPSEQAGRAQGSL
                     RKEHSETGRTRDAAGAPTDPSLRRASGKAGTPTDPSARRAAGKAGAPTDPSARRTAGK
                     AGTPTDPSARRTAGKAGTPADPSLRRAAGKAQGNPAEQKPVRSADGIVRKQGGHPNGK
                     SKAMSKKKKKQKRAAFNFASGGVLIIAACVFIFSLYQLITMLIPYYSGGEEYDKIKNI
                     AITADEEGKGFTVDFDALLKENEDTVAWIRFDEPAVINYPVVKSADNNEYLTKTFTAN
                     DNKLGAIFVDMRNSNDFSDKNTFIYGHHLNVGGEMFSELLKYENESFCKKHPNFYIYT
                     PDGKVRTYKV"
     gene            complement(1222..1857)
                     /locus_tag="ACZPD4_04275"
     CDS             complement(1222..1857)
                     /locus_tag="ACZPD4_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HIR1378320.1"
                     /translation="MDVRRMRNEALGKRVVKALESRNMEAYYASTKEEAVQKALELME
                     EGSSVTMGGSMSVRESGLLDAINAGNYEFYDRDQAATPEERQEIALKAFTCDWYLGSV
                     NAMSENGVFVNIDGNANRVAAYAFGPKNVLLIVGMNKVVKSEEDAVQRARNEAAPINS
                     QRFGLETPCVKNGSCFDCKSSDCICCQVLVTRFSRIPKRFKIILVDDVLGF"
     gene            complement(1970..2731)
                     /locus_tag="ACZPD4_04280"
     CDS             complement(1970..2731)
                     /locus_tag="ACZPD4_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR1378321.1"
                     /translation="MKKLTIGHLYPDLLNLYGDRGNIQCFRKRLEWRGMEAEVIPFLS
                     GEKIDFSKLDIVLLGGGSDREQELVCGFLKDIKDDFKAYVEDGGVVLAVCGGYQLLGK
                     YYKTNKKTIEGLSILDITTEWQPERLIRNIVLNSPLFEQPVVGFENHGGRTYIGDHTP
                     FGKVFYGLGNTGKSGYEGVIYKNVIATYLHGPLLPKNPQVCDYLLEKALQRKYGEEVK
                     LEPLKDETEKKANSYIADRYSDKKYVLRETVKRHT"
     gene            complement(2728..4083)
                     /locus_tag="ACZPD4_04285"
     CDS             complement(2728..4083)
                     /locus_tag="ACZPD4_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIR1378322.1"
                     /translation="MRLRSKFAVKCAKATSSLIKKLNRGSGVTLPGYVARLIDPNILK
                     ELSGQVRKKTIVTMGTNGKTTTNAILCKALEHEGQKVIINRTGANMLNGIISAFVLAT
                     DKHGNLDADYACIEVDEIASVGVLPKIEPDCALLTNISRDQLDRFGEVDITYNKLMTA
                     VTSVPKTTLIINCDDILSYSLAQESKNAFVTYGISEQIFDDVSRSEIRESIFCRKCGK
                     KLEYQFFHYGQLGLYHCPNCGWNRPNPEYTAEHVVREDELYSFDMDGIHFDPQIRTPY
                     NIYNTLSAYAALKTMGAGYEHFKEMIEAFDYGNNRESIFNINGARVQLHLAKNPIGFQ
                     QKVSHVLRDPNPKDIIIQINDTYQDGEDVSWLWDVDFQYLAESNARKIITAGTRKYDM
                     ALRLKYEDVSCESTDDMRASVLECVEQGTKNLYVIVNYSGLYQTNHMLTELEGKGGTE
                     R"
     gene            complement(4104..4979)
                     /locus_tag="ACZPD4_04290"
     CDS             complement(4104..4979)
                     /locus_tag="ACZPD4_04290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1378323.1"
                     /translation="MIKVIASDMDGTLLCDDHRIAPDTIAAVKEACAAGIRFMVATGR
                     GYEGAMAELDGTGIVCDYILGSGAEVRDPQRKIVKECGIGMELCREICEEVKEFPISI
                     VFLTNEGDYRIGTEKEVEESIIAEMQLFHVNMTREEVVKTSEYRRAIESVKVVSDFEE
                     IEKAGIPVFKLFMFSHDLELVRRVTKHLEKNPKIAVASSFESNVEITDARAQKGPILK
                     EYIESLGYTMDEVMVLGDSLNDYSMMSMDFGATVAMGNAVSELKKAAKYITKSNDELG
                     VAYAIRELLKKQKNK"
     gene            complement(5180..6553)
                     /locus_tag="ACZPD4_04295"
     CDS             complement(5180..6553)
                     /locus_tag="ACZPD4_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378324.1"
                     /translation="MKKENDFGRDSIPLLVLKISIPFMFAQFVNVLYSIVDRIYIGNI
                     PVIGADSLAGAGVCAPIITLLSSFGTLIGIGGSVLFSVRLGAGDEKSAKQILANSFSM
                     LLIFSGILTIVFLLTKDYLLRWFGASADIFPYANTYMTIYTLGTFFALLSMGMNYFIT
                     AQGYPGLGMLTTLIGAIVNIVLDPVFIFGLEMDIAGAAVATVIAQMSSCIFVLYTLKR
                     KKMRIPLTLVKPEREIGKRIVKIGFSPFLILASDSVIIIALNAVLQYYGGADYGDTLI
                     TAATIVQSYMLLITAPMLGVTGGSQPLISYNYGANRADRIRKTVLWVLMLCVFFTAVM
                     FIISRVAPQYFVRIFTDNAEYRELSVWGIKVFTLMVVPLSLQYVFVDALTALGMTKLS
                     LGMSVVRKVFYFSSTCFLPALFTAKDAFYAEPVADILAASVTSVVFFFVYRKYLKGSG
                     RLGEIKL"
     gene            complement(6770..7231)
                     /locus_tag="ACZPD4_04300"
     CDS             complement(6770..7231)
                     /locus_tag="ACZPD4_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1378325.1"
                     /translation="MLMPSIFGENLFDDDWMDFPFERFDRDFWGKKNPLYGKNAKNMM
                     KTDIREHEAGYELDIDLPGFKKDEITVDLENGYLTISAAKGLDKDEEDKKGKYIRKER
                     YAGTMQRSFYVGDAITHEDIKAKFENGILSLTIPKKDAKAVETKKSIAIEG"
     gene            complement(7494..7898)
                     /locus_tag="ACZPD4_04305"
     CDS             complement(7494..7898)
                     /locus_tag="ACZPD4_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378326.1"
                     /translation="MVEHTICSTCQADISGVAGQHLDETMHAGYYSGWRKEQTGTKTY
                     TVNHPEQGHYESIVTGYNVAVEQFNEMEVYNHITDWFRIEKRLRRGIWFRLCDGSDFF
                     KNDYRTTRYGPHDLHSFGRGYRLISEKNDLHL"
     gene            complement(8365..>8823)
                     /locus_tag="ACZPD4_04310"
     CDS             complement(8365..>8823)
                     /locus_tag="ACZPD4_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIR1378327.1"
                     /translation="SKRTNYLVRTYEDKLEELGFEQEGNNYCLTDSDFKYDIYMFDRP
                     SIWKWNQEMSDREKFIVATLLVKNSDLEENPGITIRKTGDFITLYISRENHLADDGST
                     KPKEYSLDRNGQIKHKEKMEADDYEVYAKLKEEIENGVKKLNEMYSNIYE"
BASE COUNT         2282 a         2330 c         1418 g         2793 t
ORIGIN      
        1 caccttatat gttctaacct ttccatccgg tgtatatatg tagaagtttg gatgcttctt
       61 acagaaagat tcattttcgt atttaagaag ctccgaaaac atttctcctc cgacgttaag
      121 atgatgaccg tagataaacg tgtttttgtc tgaaaaatca ttactgtttc tcatatctac
      181 aaaaatcgca ccaagcttat tgtcatttgc cgtaaacgtc tttgtcagat actcattatt
      241 atccgcgctc tttacaacgg gataattgat caccgccggc tcatcaaagc gaatccatgc
      301 aaccgtgtct tcgttttctt tcagcaaagc gtcaaaatca acagtaaaac ctttcccttc
      361 ttcatccgcc gtaatggcaa tgtttttaat cttatcgtac tcttccccgc cggagtaata
      421 cggaatcagc atcgtgataa gctgatacaa cgaaaaaata aacacgcacg ccgctataat
      481 aagcactcct ccggaagcaa aattaaatgc tgcccgtttt tgtttctttt tcttttttga
      541 catcgccttt gattttccgt tcggatgacc gccttgcttt ctgacaattc catctgccga
      601 tctgaccggc ttctgttctg caggatttcc ctgagccttt cccgccgctc ttcttaagga
      661 cggatctgcc ggcgttcccg ctttccccgc tgttcttctt gcggacggat ctgtcggcgt
      721 tcccgctttc cctgccgttc ttcttgcgga cggatctgtc ggcgctcccg ctttccctgc
      781 tgctcttctt gcggacggat ctgtcggcgt tcccgctttc cctgacgctc ttcttaagga
      841 cggatctgtc ggcgctcccg ctgcatcccg cgttcttcct gtttcagaat gttcctttcg
      901 cagacttcct tgcgcacgcc ctgcctgttc agacggtttc ctcacacttc ccgactctcg
      961 tctcatcgga gtaaccgttc cgcccggcgt tgttcttctg gtcggatcgc cgctttccga
     1021 tgttcttcgt acagatgccg aactgctttt tcctgacggt cttcctgctg aggcgggagc
     1081 gccgtttccc gacggtcttc ttcccagacc ttcatttgcc cgcgcgcttc cctcagcatt
     1141 cccttctctt cgcaccgtac gttttcgcgc gttttgatcc attgtatttc tttcatttct
     1201 gtccataggt aatttcctct cctaaaatcc caaaacatcg tctacaagaa ttattttaaa
     1261 ccgcttcgga attctgctga atcttgtgac caagacctga cagcagatgc agtctgaact
     1321 cttgcagtca aaacagcttc cgttttttac gcatggagtc tcaagtccga atctctgtga
     1381 attgatcggc gcagcttcgt ttctcgccct ttgcaccgca tcttcttctg acttcaccac
     1441 tttattcatt cctacgataa gcagtacgtt tttcggtccg aacgcatatg ccgccacacg
     1501 gttcgcattt ccgtcaatat taacaaatac tccgttctca ctcatagcat tgacacttcc
     1561 gaggtaccag tcgcatgtaa acgctttcaa tgcaatctcc tgacgttctt cgggtgtcgc
     1621 cgcctgatcc ctgtcataaa actcataatt tcccgcattg atcgcatcaa gaagtcccga
     1681 ttcccgaacc gacatcgagc cgcccattgt aaccgagctt ccctcctcca tcagctccag
     1741 cgctttttga acagcttctt ctttcgttga tgcataataa gcttccatat ttcttgattc
     1801 aagcgctttc acaacacgtt tgccaagcgc ttcatttctc atccgtctta catccataag
     1861 aaatacttcc tttcttttat tttatattta cagcaacaag cgtagtgttc ccgccagaac
     1921 ttccacctgc cggaaaatct gtctttctgt cacactctct gcagccggat caggtatgtc
     1981 tttttaccgt ttcccgaagc acatattttt tatcgctgta cctgtcggca atataactgt
     2041 ttgctttctt ttccgtctcg tctttcagcg gctcaagctt cacttcttca ccgtattttc
     2101 tttgaagcgc tttttcaaga agataatcac atacctgcgg atttttcgga agaagaggtc
     2161 cgtgcaaata tgttgcgatc acatttttat atataacgcc ttcataacct gattttcctg
     2221 tatttcccaa gccgtaaaac actttcccga acggagtgtg atcaccgata tatgtccttc
     2281 ctccatgatt ttcaaatccg actacaggct gttcaaaaag cggactgttc aacacgatat
     2341 ttctgatcag tctctccggc tgccattctg tcgttatatc aagaatcgaa agaccttcta
     2401 tcgtcttttt atttgtcttg taatatttcc cgagaagctg atatcccccg cacacagcga
     2461 ggacaactcc cccgtcttca acatatgctt taaaatcatc tttgatgtct tttaggaaac
     2521 cgcatacaag ttcctgctct ctgtcagatc cgccgcctaa gagcacgata tccaacttag
     2581 aaaaatcaat tttttcacct gatagaaacg gaatcacctc tgcctccatc ccccgccatt
     2641 caagacgttt ccgaaaacac tgtatatttc ctctgtcacc gtataagttt aagagatccg
     2701 gatataaatg tccgattgta agtttcttca tcgttccgta cctcctttcc cctctaattc
     2761 agtcagcata tggttcgtct gatacaatcc ggagtagttc acgataacat aaagattttt
     2821 cgtgccctgc tctacacact caagcaccga tgcacgcata tcgtctgtag attcgcaaga
     2881 aacatcttcg tatttcaacc gaagcgccat atcatacttc cttgttcccg ccgtaataat
     2941 ctttcttgcg ttactttccg caagatactg aaaatccaca tcccaaagcc atgatacgtc
     3001 ttccccatcc tgatacgtat cattgatctg aatgataata tctttcggat tcggatctct
     3061 gagtacatgc gatactttct gctgaaaacc gatcggattt ttcgcaaggt ggagctgcac
     3121 tcttgcaccg tttatattaa aaatactttc tctgttattt ccataatcaa acgcctctat
     3181 catctcttta aaatgttcat atcccgctcc catcgtttta agagccgcat acgccgacag
     3241 agtattgtaa atattataag gtgtccttat ctgcggatca aagtggattc cgtccatatc
     3301 aaatgaatac aattcatctt ctcttacgac atgttcagcc gtatattccg gatttggtct
     3361 gttccatccg cagttcggac aatgatacaa ccctaactga ccataatgga aaaactgata
     3421 ttccagtttc tttccgcatt tgcggcaaaa aatgctctcc cgtatttcag atcgtgaaac
     3481 atcgtcaaat atctgctcgc tgataccata tgtgacaaat gcatttttgc tttcctgcgc
     3541 caaagagtaa gataaaatgt catcacaatt tataataagc gttgtcttcg gaacgcttgt
     3601 caccgctgtc atcaatttat tataagtaat atcaacttca ccgaaacgat caagctgatc
     3661 tcgcgaaata tttgtcagca gcgcacagtc tggctctatt ttcggcagca cccctacaga
     3721 tgcgatctca tctacttcta tgcatgcata atccgcatcc agattgccat gcttgtctgt
     3781 tgcaagaaca aacgccgaaa taattccgtt taacatatta gcgcccgtac ggttaataat
     3841 cactttctga ccttcatgct ctaacgcctt acataaaatc gcgttcgtcg ttgtctttcc
     3901 gtttgttccc attgtgacga tcgtcttttt tctaacctga cccgataatt ccttcagtat
     3961 attcggatcg atcagacgcg ccacatatcc cggaagcgtc accccgcttc cacggtttaa
     4021 ctttttgatc agactgctcg tcgctttcgc gcatttcaca gcaaatttac ttctcaatct
     4081 catacttgaa ccaatttccc tttttattta tttttctgct tttttaacag ttcccgaatg
     4141 gcatacgcaa caccaagctc atcatttgac ttcgtaatat attttgccgc ttttttcaat
     4201 tccgaaaccg catttcccat cgctaccgtc gctccgaaat ccattgacat catcgaataa
     4261 tcattcaaac tgtctccgag aaccataacc tcatccatag tatatccaag cgactcgata
     4321 tattccttca atatcggacc tttctgcgct cttgcatccg tgatctccac atttgattca
     4381 aatgatgaag cgactgcgat tttaggattt ttttcaagat gttttgtcac cctgcggact
     4441 aactcaagat catgtgaaaa cataaatagt ttaaacaccg ggatacccgc tttctcaatc
     4501 tcttcaaaat cagaaacaac ctttacactt tctatcgccc tgcgatattc gcttgttttc
     4561 acgacctctt cccgggtcat atttacatga aaaagctgca tctccgctat aatactttct
     4621 tcgacttctt tttccgttcc gatacggtaa tcgccttcat ttgtaagaaa tacgatcgat
     4681 atcggaaatt ctttcacctc ttcgcagatc tctctgcaca actccattcc gatgccgcat
     4741 tcttttacaa tttttctctg cggatctctg acttccgctc cgcttcccaa tatataatca
     4801 caaacaattc ctgttccgtc aagctctgcc atagcgcctt cataacctct gcctgtcgcc
     4861 accataaacc ggatgcctgc cgcacacgct tcctttactg ccgcgatcgt atccggagcg
     4921 attctgtggt catcacaaag aagcgttcca tccatatcgc tcgctatcac tttgatcata
     4981 tcttcatatc tccaatctat atacaatagt tcagccatac agtgtacgta gagcataaaa
     5041 catgcttgca tgtttttacc gacggacatc tgtgtgcggt tacttgctat tctatctatt
     5101 ataaagtttt tccccgcatt ttacaacaca aatcgcccta acctgcacag tcgtttttcc
     5161 ataaccgtca cagtcgtttc tacagtttga tttccccaag tcttccgctg cctttcagat
     5221 atttcctata tacaaagaaa aaaacaacgc ttgtcaccga tgccgccagt atatccgcca
     5281 ccggctccgc ataaaaagca tctttcgccg taaacaacgc cgggagaaaa catgttgatg
     5341 aaaagtaaaa aacttttctt acaacagaca tcccaagaga caacttcgtc atgccaagag
     5401 cagtaagcgc atcaacaaaa acatactgca gacttaacgg aacaaccatc agagtaaaca
     5461 ctttgatccc ccacacggac agttcccggt actctgcatt atccgtaaaa atacgaacaa
     5521 aatactgcgg agccactctt gaaataataa acatcaccgc cgtaaagaaa acacagagca
     5581 tcaacaccca gagcactgtt ttccgaatac gatccgctct gtttgctcca taattataac
     5641 taataagcgg ctgcgaccct cctgtcacgc caagcatcgg cgccgtgatc aaaagcatat
     5701 agctttgcac gatcgtagcc gccgtgatca acgtatcccc gtagtctgct ccgccgtaat
     5761 actgcaaaac agcattcagc gcaatgatga tcacactgtc tgatgccaga atcaaaaacg
     5821 gagagaaacc gatcttcacg attcgttttc caatctcccg ctcaggcttc accagcgtca
     5881 gcggaatacg catcttcttt ctcttcaatg tataaagaac aaagatgcat gatgacatct
     5941 gtgcaatcac cgtagccact gccgcccccg caatatccat ttcaagtccg aaaataaata
     6001 ccggatcaag cacgatattt acgattgctc cgatcagtgt cgtcagcatt ccaagccccg
     6061 gatatccctg tgccgtaata aaataattca tccccattga caaaagagcg aaaaaagttc
     6121 caagcgtgta aatagtcata taagtattgg catacggaaa tatatctgcg cttgccccaa
     6181 accatctcag taaataatct ttcgtaagta aaaatacaat cgtcaatatt cccgaaaaaa
     6241 taagcagcat agaaaaactg tttgccagta tctgcttagc gcttttttca tctcccgccc
     6301 cgagacgtac cgaaaacaag acggaacccc cgataccgat cagcgttccg aacgatgaca
     6361 acaatgtaat gatcggagca catacccccg cgcctgccag cgagtcagcc ccgatcaccg
     6421 gaatatttcc tatatagatt ctatccacaa tactgtacag cacattaaca aattgcgcga
     6481 acataaacgg aattgaaatt tttaatacaa gcaaaggaat agaatccctg ccaaaatcat
     6541 tttctttttt cataactcat ataaccctct ccctaagatt tcaggttata tattatcact
     6601 ttctcacaga tataaaaacc gcgaaaagtg atttttctaa aactttttct cgattttatt
     6661 cctgcaatat ccttacatcc gctcttgcat gtaaaaacgg cagtgaacgg gaacagcaca
     6721 ctcgccgttc ccgttcacta ccgtcaaaaa cttgtcaaaa gaaatgaatt tatccttcaa
     6781 tcgcaatgga tttcttagtc tctactgctt tcgcatcctt tttcggaatt gtcaggctca
     6841 aaataccgtt ttcaaatttt gctttaatat cttcgtgagt gatcgcatcg cctacataaa
     6901 aactcctctg cattgtacct gcataacgct ctttccgaat gtattttccc ttcttatctt
     6961 cctcgtcctt gtcaagtcct ttcgccgccg agatggtcag ataaccattt tcaagatcca
     7021 cggtaatctc gtccttttta aatcccggaa gatcaatatc caactcatat cccgcttcat
     7081 gctcacgaat atctgtcttc atcatgtttt tggcattctt accatacagc ggatttttct
     7141 ttccccagaa atctctgtca aatctctcaa acgggaaatc catccaatca tcgtcaaaca
     7201 aattctctcc aaaaatacta ggcatcaaca taagtcatat ctcctttcaa aacacttttg
     7261 aatttattgt tttctttgtt atatctgtaa tatagcaccc gttattagca ctgtcaagag
     7321 gtgagtgcta atatttttgc tatttttttg caaacccttt aaaatcaatg gtttgcagta
     7381 tcttatttct tttgaaattg tgattttttc tgttttgaca ccaatttgac accatttgcg
     7441 ctaaaactac aagccatgca agcgaaaaat gagctcccgt tgagagtttg atctcataag
     7501 tggagatcat ttttttcgct tataaggcga tagccgcgac cgaatgaatg caggtcatga
     7561 ggtccatagc gagtagttct gtaatcattc ttaaagaaat ctgagccgtc acataatctg
     7621 aaccaaatcc ctcttcgcaa tctcttttcg atcctgaacc agtctgtgat atggttataa
     7681 acttccattt cattgaactg ctccacagct acattatatc cggtcacaat cgattcgtaa
     7741 tgcccttgtt ctggatgatt aactgtatac gtttttgttc ctgtttgctc ttttctccag
     7801 cccgagtaat atcccgcatg cattgtctcg tctaaatgtt gtcctgcaac tcctgagata
     7861 tcagcttgac atgtgctaca aattgtatgt tctaccattt catatactgg ttcattgaca
     7921 gtttctgtcc aagcttcctg atcgactacc cattctcctg tatagatcgg aatactataa
     7981 tcatgagtat gtaccggagc tgtattttga gttccgccgt ttcctgagtt ccctccatta
     8041 gattcccctg ttgtttggga agcgttattt ttctgtgtag aagcagaagc atccggttga
     8101 gatgctgtct gcttctcatc ctattctttc ttgtaccgaa tttcggtaca agaacctgtt
     8161 ctaaaaaaca ccacacattt attatacttg ttatgcaagt atttgcatgc ccatattata
     8221 gctacaaaat attagttcaa tcggattttt gcaaccgtac accacaactc cgcaacactt
     8281 tcttatagtt tagattaatc aaaagaaggg tgtcaagcat tcaacaccct attattcgac
     8341 cttgcaatca ttatacaaat atttttattc ataaatgttt gaatacattt cattgagttt
     8401 tttcactcca ttctctatct cctctttcag tttagcatat acttcataat catctgcttc
     8461 cattttttct ttatgtttta tctgaccgtt tctatcaaga ctatactctt ttggttttgt
     8521 agaaccgtcg tctgccaaat ggttttctct tgaaatataa agtgttataa aatcgcctgt
     8581 tttccgaatt gtaatacccg gattttcttc caaatcgcta ttctttacga gaagtgtggc
     8641 tacgataaat ttctctctgt cactcatttc ctgattccat ttccatatag aaggtctgtc
     8701 aaacatataa atatcatact taaaatcgga atctgtgagg caataattat taccttcttg
     8761 ctcaaatcca agttcctcca acttatcttc atatgtccgc acaagatagt ttgtccgttt
     8821 cga
//
LOCUS       DBMDXF010000072         8627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2191_length_8627_cov_2.024982, whole genome shotgun sequence.
ACCESSION   DBMDXF010000072 DBMDXF010000000
VERSION     DBMDXF010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_8627_cov_2.024982"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            9..1319
                     /gene="rho"
                     /locus_tag="ACZPD4_04315"
     CDS             9..1319
                     /gene="rho"
                     /locus_tag="ACZPD4_04315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR1378328.1"
                     /translation="MISLDEQEDQEKLSEQKESSQEHLKEMQNQNREEKAASELKENK
                     EHREAKDKKPETDMEQLDSGCSVSGILEVMPDGFGFIRSDNYLPGENDVYVSPAQIRR
                     FGLKTGDILSGNTRIKTQSEKFSALLYVTTVNGYHPAEAAKRPNFEELTPIFPKDRIR
                     LEQPGSSTAMRIMDLISPIGKGQRGMIVSPPKAGKTTLLKEVALSVQKTEPDMHLLIL
                     LIDERPEEVTDIKEAIEGPNVEVIYSTFDELPEHHKRVSEMLIARAKRLVEHGRDVMI
                     LLDSITRLSRAYNLTVPPSGRTLSGGLDPAALYSPKRFFGAARKMREGGSLTILATAL
                     VETGSKMDDVVYEEFKGTGNMELMLDRKLQEKRVFPAIDIPRSGTRREDLLLNKEEQE
                     ASYIIRKALNGMKAEEAADNLINMFSRTKSNAELVQQVIRQKFI"
     gene            1410..1610
                     /gene="rpmE"
                     /locus_tag="ACZPD4_04320"
     CDS             1410..1610
                     /gene="rpmE"
                     /locus_tag="ACZPD4_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR1378329.1"
                     /translation="MREGIHPEYHQATVTCNCGNTFVTGSTKENIHVEICSKCHPFYT
                     GQQKAAQARGRVDKFNKKYGIK"
     gene            1771..2694
                     /locus_tag="ACZPD4_04325"
     CDS             1771..2694
                     /locus_tag="ACZPD4_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1378330.1"
                     /translation="MRSSNIGGQAVLEGIMMRNGGKYSVAVRKPDGEIESEVWDYHSV
                     IPWKVLLKIPFIRGIFNFVDSLVLGMKTLMYSASFFEEEEEGELLTKEEAQKREKKEK
                     IIMNITVVISLLAAAALFMILPYVLSGLLKRVTSSRVAVTVFEGVIRVAIFLIYIFLI
                     SRTKDIKRTFMYHGAEHKCINCIEHGLELNVENVRKSSKQHKRCGTSFLFFVMIVSVI
                     FCFFITAQSQVMRIVIRLALLPLIAGVSYEIIKLAGNSDNPVVNLLSKPGMWVQNMTT
                     KEPDDSMIEVAICAVEKVFDWREWQEKNNEL"
     gene            2684..3547
                     /gene="prmC"
                     /locus_tag="ACZPD4_04330"
     CDS             2684..3547
                     /gene="prmC"
                     /locus_tag="ACZPD4_04330"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1378331.1"
                     /translation="MNYKEAYAWGVELLEKACVEESKIDAWYLLEYVTKISRAVFYAY
                     PEKELTEEEKIQYEECIKRRAERIPLQHITGVQEFMGLPFLVNEHVLIPRQDTEVLVE
                     QALCLLEKEKKHKEIVRILDLCTGSGCILLSMLHYVRRKRKIDIYGVGSDISKEALAV
                     AVENAKKLGIEAKFVQGDLFENVEGSFDMILSNPPYIRTSEIERLQEEVRFHDPIKAL
                     DGKEDGLYFYRIIVKESRQYLKRGGWLIFEIGSDQAEAVKTLLQEAGYENIEVKKDLA
                     GLDRVVYGMYS"
     gene            3659..4729
                     /gene="prfA"
                     /locus_tag="ACZPD4_04335"
     CDS             3659..4729
                     /gene="prfA"
                     /locus_tag="ACZPD4_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1378332.1"
                     /translation="MFDRLEDLLIRYEEVMGELQEPGVADDPVRFRKLMKEQSDLAPI
                     VEAYNEYKQCRQNIEDSLAMLEEESDEEMRELAKEELSDSRKRVEELEQELKILLLPK
                     DPNDDKNVIVEIRAGAGGDEAALFAAEIYRMYVHYADSRRWKTEMISLNENGIGGFKE
                     CVFMITGQGAYSRLKYESGVHRVQRVPETESGGRIHTSTITVAIMPEAEEVDVVIDEK
                     DIRIDVMRASGNGGQCVNTTDSAVRLTHYPTGIVIYSQTEKSQLQNKEKAFRLLRSKL
                     YDLELEKQQASEAEARRSQIGTGDRSEKIRTYNFPQGRVTDHRIKLTLHKLDTILNGD
                     LDEIIDSLIAADQTAKLAKMEG"
     gene            4735..5634
                     /locus_tag="ACZPD4_04340"
     CDS             4735..5634
                     /locus_tag="ACZPD4_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HIR1378333.1"
                     /translation="MTEPTFKRPELEDKEIISSYFEKAQSRSCERTFVNVYLWSRHYK
                     VQYAIIEDTLVFRDSGKNLSFTYPAGEPENVKKALEFLMEYCKEKDVPFILYNVTPHM
                     FAQLDKWYPKKFFIEYNRDLADYVYETEKLASLAGKKLHGKRNHINKFKALYPDWSYE
                     PLNDDNVEDCFQMALKWRNKNGCEDDPEKNAEMCVTLNSLRLYKELGLKGGVLKADGK
                     IVAFTVGEPVSDDTFVVHIEKAFAEVDGAYPMINQQFVQHECMEYEYVNREEDTGAEG
                     LRKAKLSYRPAFLEEKGIVKEME"
     gene            5640..6827
                     /locus_tag="ACZPD4_04345"
     CDS             5640..6827
                     /locus_tag="ACZPD4_04345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1378334.1"
                     /translation="MISKKMENMVANSSAIRAMFEEGNRLAKIYGAENVFDFSLGNPN
                     VPAPEEVKNAIIELLNEEDPIALHGYTNSNAGYADVREAVAQSLNERFGTEFEGKNIV
                     MTVGAAGGLNVILKSLLNPADEVIVFAPYFGEYRSYVSNYDGVLVEVSPDTETFQPKL
                     AEFEEKITAKTKAVIVNTPNNPTGVVYSEETIEKMAEILEKKQKEFGTDIYLISDEPY
                     RELAYDGVEVPYLTKYYANTIVGYSYSKSLSLPGERIGYLVIPSEASDSEDLISAASV
                     ATRILGFVNAPTLQQKVVKACLNAKTDISYYDRNREALYNGLKELGFECVRPEGAFYL
                     FVKSPVEDEKAFCAAAKKYNILIVPGSSFGCPGYVRIAYCVAYETIVNSLPKFKELAG
                     EYK"
     gene            6864..7928
                     /gene="hisC"
                     /locus_tag="ACZPD4_04350"
     CDS             6864..7928
                     /gene="hisC"
                     /locus_tag="ACZPD4_04350"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR1378335.1"
                     /translation="MTVRKELLNNIRIVDPYVPGEQPRQKVVKLNTNENPYPPAPEVK
                     AVFDELKEDEFRLYPDPTSGKLIKALADQLKVGEDQIFVGVGSDDVLSLCFLTFFNSE
                     KPILFPDITYSFYQVWANLYRIPYVCPKLDGKFRIVKEDYYKVNGGIIFPNPNAPTAI
                     YEEVDFIEDILRHNRDNIVIIDEAYVDFAGKSAISLIDRYDNLIVTQTFSKSRSMAGM
                     RIGYAVSNPELIRCLNDVKYSFNSYTMSCAAISCGAAALENTEYFKTNISKIVQTREN
                     VKEQLRDLGFECTDSKANFLFVTHPRYDALKLFEELKEAGIYVRHWNDPKIKDYLRIT
                     IGTDEEMKIFLDFLKKKIGA"
     gene            7932..>8627
                     /locus_tag="ACZPD4_04355"
     CDS             7932..>8627
                     /locus_tag="ACZPD4_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR1378336.1"
                     /translation="MIKKLLQGMVVGIANIIPGVSGGTMMVAMGLYDKLIHAITHLKS
                     EFKNSMKLLIPIFIGAGAAIVILSRLFEFLLENYPIPTNFAFCGLIAGSLPFIFNKVK
                     GHSVSTGKIAAFLIFFGIVILMALLGDTNGKAADVSFGIINMIKLFSVGIIAAATMVV
                     PGVSGSMMLMLLGYYDTILKTINQFIDAVVAFDINGILTQCKVLIPFGIGIVIGIFLI
                     AKLIEFIFSKAEIH"
BASE COUNT         2780 a         1407 c         2211 g         2229 t
ORIGIN      
        1 tcgaagcgat gatctctctt gacgagcagg aagatcagga gaagctttcg gaacaaaaag
       61 aaagttcgca ggagcatttg aaagaaatgc aaaatcaaaa tagagaggaa aaagcagctt
      121 ctgaattgaa agaaaataag gaacatagag aggcgaagga taagaagccg gagacagata
      181 tggaacagct tgacagcgga tgctcggtaa gcggaatatt agaggtcatg cccgacggtt
      241 ttggatttat cagaagtgac aactatcttc cgggagaaaa tgacgtatat gtctcacctg
      301 cacagatccg ccggttcgga cttaaaacag gagatatatt gtctggaaat acaaggatta
      361 aaacgcagag tgaaaaattc agcgcccttc tttatgtaac tacagtcaac ggatatcacc
      421 cggcggaagc tgcgaaaaga ccgaactttg aagagttgac gccgatattt ccaaaagaca
      481 gaatccgtct ggaacagccg ggaagcagta cggcgatgcg gattatggat ttgatttcgc
      541 cgatcggaaa aggtcagaga gggatgatcg tttctccgcc gaaagcggga aaaacaactc
      601 ttttaaaaga agttgcgctt tcggtacaga aaacagaacc ggacatgcac cttcttatat
      661 tactgatcga tgaacggccg gaggaagtaa ctgatattaa agaagcgatc gaagggccga
      721 atgtagaggt aatatattct acgtttgacg aacttccgga acatcataaa agggtatcgg
      781 agatgttgat cgcaagggca aagcgtctcg tagaacatgg ccgggatgta atgatccttt
      841 tagatagtat tacaagatta tcacgggcat ataacctgac cgttccgcca tcgggacgaa
      901 cattgtcggg aggtcttgat ccggcagctc tttacagtcc gaagcgcttt ttcggagcag
      961 cccgaaaaat gcgggaaggc ggaagtctta ctattctggc cactgctctc gtagaaacgg
     1021 gaagtaagat ggacgatgtc gtatacgaag agttcaaagg aacaggaaat atggaactta
     1081 tgcttgatcg aaaacttcag gagaaaagag tgtttccggc aatcgatatt ccgcgttccg
     1141 gaacgagaag agaagatctt cttcttaata aagaggaaca agaagcaagt tacattatcc
     1201 ggaaggcgtt aaacggaatg aaagcggaag aagccgcaga caatctgatc aatatgtttt
     1261 cgaggacaaa atcaaatgca gaattagtgc agcaggttat tcgtcagaag tttatctaaa
     1321 aaatagttgc aaaaggaaat aacttgtgat acaatgagga agctgtttag agattataat
     1381 aactattttg aaatagagag gtgaaaatca tgcgcgaagg aatccatcca gaatatcatc
     1441 aggcaactgt gacttgcaac tgtggaaaca catttgtaac aggttctaca aaagagaaca
     1501 tccacgttga aatctgctcc aaatgccatc cgttctatac aggacagcag aaagcagcac
     1561 aggcacgcgg ccgtgttgat aagtttaata aaaaatacgg tattaaataa gaagcgaatg
     1621 aagcaggctg aggtgggatc atctcagcct cttttcttta ctgagaatac gttagttttt
     1681 cagtaattaa aaatctgtac aggtcagact gagaaagcaa ttctcttagt taagattccg
     1741 gcagacagta aaggagaaag cagggaaatt atgcgatcat cgaatattgg cggacaggca
     1801 gtgctggaag gcattatgat gagaaacggc ggaaagtatt ctgtggcagt acgaaagccg
     1861 gatggagaga tagaatcaga agtgtgggat tatcatagtg tgattccgtg gaaagttctt
     1921 ctgaaaattc cttttataag aggaatattt aattttgtgg attctcttgt tcttggaatg
     1981 aagacgttga tgtattctgc ttcgtttttt gaggaagaag aggaaggcga acttttgacg
     2041 aaagaagagg cgcaaaagag agaaaagaaa gagaagatca ttatgaacat tacggtagtc
     2101 atttcgttgc tggcggctgc ggcattgttt atgatactgc cctatgtgct gagcggactt
     2161 ttgaaacggg tgacgtcatc ccgtgttgcg gtgactgttt ttgaaggtgt gatccgtgtt
     2221 gcgatctttt tgatttatat ttttcttatt tcaaggacaa aagatataaa aagaaccttt
     2281 atgtatcacg gggcagaaca taagtgtatt aactgtatcg aacacggatt agaactgaat
     2341 gtggaaaatg tgaggaaaag ttcaaaacag cacaaacgat gcggaacaag ttttctcttt
     2401 tttgtgatga tcgtaagtgt gatattttgc ttttttatta cggcgcagtc acaggtgatg
     2461 cgcattgtta tcaggttggc gcttcttcct cttattgcag gtgtttctta tgaaatcatc
     2521 aaattggcag gcaacagtga caatccggtt gtgaatcttt tgagtaagcc gggaatgtgg
     2581 gttcaaaata tgacgacgaa agaaccggat gacagtatga tcgaagtggc gatctgtgcg
     2641 gtagagaaag tgtttgactg gcgggagtgg caggagaaga acaatgaatt ataaagaagc
     2701 gtatgcatgg ggcgtcgaat tgttagaaaa ggcatgcgtc gaagagtcaa agatcgatgc
     2761 atggtattta ttagaatatg tgacaaagat cagcagggca gtattttatg catacccgga
     2821 aaaagaattg actgaagaag aaaaaataca gtatgaagag tgtatcaaaa ggcgggcaga
     2881 gaggattccg ctccaacata ttaccggggt acaggaattt atgggacttc cgtttctggt
     2941 aaacgaacat gtacttattc cccgtcagga tacagaagtt cttgtagaac aggctttgtg
     3001 tctgcttgaa aaagaaaaga aacataagga aatagtccgg attcttgatc tttgtaccgg
     3061 atcaggctgc attcttttga gtatgctgca ttatgtaaga aggaaacgaa aaatagatat
     3121 ttacggcgtc ggctcggata tttcaaaaga agcgctggct gttgcggttg aaaatgcgaa
     3181 aaagctcggt atagaagcga agtttgtgca aggagatctg tttgaaaacg tagaaggaag
     3241 ttttgatatg attctttcaa acccgcctta catacggaca tctgaaatag agcggctcca
     3301 agaggaagtc agatttcacg atcctatcaa ggcattggac ggaaaagagg acggtcttta
     3361 tttttatcgg atcatcgtga aagaaagcag acagtattta aagcgcggcg gatggcttat
     3421 atttgagatc ggaagcgatc aggcggaagc ggtaaaaacg ctgcttcaag aggctgggta
     3481 tgaaaatatt gaagtgaaaa aagatcttgc aggacttgac cgcgtcgtat atggcatgta
     3541 tagttgaaaa taagtttgcc gcaagcccat tagctttaga cagtgggtag ttttacaaag
     3601 tgtatagaaa atatacagga cagtatacag ataaggtgta caggaaagga taagagaaat
     3661 gtttgacaga ttagaagatt tgttaattcg atatgaagaa gttatgggag agttacagga
     3721 gccgggtgtg gcggacgatc ctgtaaggtt tagaaaactg atgaaagagc aaagtgatct
     3781 tgcgccgatc gtagaggctt acaatgagta caagcagtgc aggcagaaca tcgaagacag
     3841 tctggcgatg cttgaggaag agtctgacga agagatgaga gaacttgcga aagaagagtt
     3901 gagcgattcg agaaaacgtg tagaagaatt ggaacaggag ctgaagatcc ttctgctccc
     3961 gaaagatccg aatgatgata agaacgttat cgttgagatc cgggcaggcg caggcggtga
     4021 tgaagcggcg ctttttgcgg ctgagatcta ccgtatgtat gttcattatg cggacagccg
     4081 aagatggaag acagagatga tctctttgaa cgaaaacgga atcggaggat tcaaagagtg
     4141 cgtattcatg ataacaggtc agggagctta ttccagattg aaatatgaaa gcggagttca
     4201 cagggttcag cgcgttccgg aaacagagag cggcgggcgt atccatacat ctacgattac
     4261 cgttgcaatc atgccggagg cagaagaagt tgacgtagtg atcgatgaga aagatattcg
     4321 tatcgacgtt atgcgtgcgt cgggaaacgg aggacagtgt gtcaatacaa ccgattccgc
     4381 agtccgtctt actcattatc ccacgggaat tgtaatttac agccagacgg agaagtctca
     4441 gcttcaaaat aaagaaaaag cattccgtct gcttcgttca aaactctacg atctggaatt
     4501 ggaaaaacaa caggcttcag aagcggaggc tagaagaagt cagatcggta cgggagaccg
     4561 ttccgaaaag atcagaacat ataatttccc gcagggacgt gttacagatc acaggatcaa
     4621 gctgactctt cataagttag atacaatact gaacggagat ttggatgaaa ttatcgacag
     4681 tctgatagca gcagatcaaa cagcgaagct tgcaaaaatg gagggataag atttatgaca
     4741 gaacctacgt ttaaacggcc ggaactggaa gataaagaaa ttatttcctc ttattttgaa
     4801 aaagctcaaa gcagaagctg tgaaagaaca tttgtgaatg tatatctatg gtcaagacat
     4861 tacaaagtac agtatgcaat catcgaagat acacttgttt ttagagacag cgggaaaaat
     4921 ctttcgttta cttatccggc gggagaaccg gagaatgtta agaaagcgct tgaatttctt
     4981 atggaatatt gcaaagagaa agacgttcct tttatattgt ataatgtaac gccgcatatg
     5041 tttgcacagc ttgacaagtg gtatccgaaa aagtttttta tcgagtataa ccgagatctg
     5101 gcagattatg tgtatgagac ggagaaactt gcttctctgg caggaaaaaa gcttcatggg
     5161 aagcggaatc atatcaataa attcaaggct ctttatccgg attggagcta tgagccgtta
     5221 aatgacgata atgtggaaga ctgcttccag atggctctaa aatggagaaa taaaaacgga
     5281 tgtgaagatg atccggaaaa aaatgcagag atgtgcgtga cgctgaattc tttgcgctta
     5341 tataaagagt tgggattaaa aggcggcgtt ttaaaagcag acggaaagat cgtagccttt
     5401 acagtcggag aaccggtcag cgacgacaca tttgtcgtgc atattgaaaa agcgtttgcg
     5461 gaagtagacg gggcgtatcc gatgatcaat cagcagtttg tacagcatga atgtatggag
     5521 tatgaatatg tgaatcgtga ggaggacacc ggagccgagg gacttcgcaa ggcgaaactt
     5581 tcttatagac cggcttttct tgaggaaaaa ggaattgtaa aagaaatgga gtgacagcta
     5641 tgatatcaaa aaaaatggaa aatatggtgg caaacagttc tgcgatccga gcgatgttcg
     5701 aggagggaaa ccgtctcgca aagatttacg gtgcggaaaa cgtatttgat ttcagtctcg
     5761 gcaatccgaa cgttccggcg ccggaagagg tgaaaaatgc gatcatcgaa cttctgaatg
     5821 aggaagatcc gatcgcgctt cacggttata caaacagtaa cgcaggttat gcggatgtgc
     5881 gcgaagcggt ggcgcagtct ttgaacgaac ggttcggcac agaatttgaa ggaaaaaata
     5941 tcgtcatgac tgtcggagcg gcaggaggat taaatgttat tttaaaatct ttgctgaatc
     6001 cggcagatga agtgatcgta tttgcaccgt atttcggaga atacagaagt tatgtcagca
     6061 attatgacgg tgtgcttgtc gaagtgtcac ctgatacaga gacatttcag ccgaaacttg
     6121 cagagtttga agaaaagatc acggcaaaga cgaaagcggt gatcgtcaat acgccgaata
     6181 atccgacagg agttgtatat tcggaagaga cgattgaaaa aatggcggaa attttagaga
     6241 agaaacagaa agaattcgga acggatattt atcttatttc ggacgagccg tatagagagc
     6301 tggcatatga cggagtggaa gttccttatc tgacgaagta ttatgcgaat acgatcgttg
     6361 gatattctta cagtaaatcg ctttctcttc cgggagaacg aatcggttat cttgtgatcc
     6421 caagtgaagc ttcagacagc gaagatttga tttcggcagc aagtgtagcg acgcgtattc
     6481 ttggatttgt caatgccccg acacttcagc agaaagtagt aaaggcatgt ttgaatgcga
     6541 aaacagatat ttcttattat gacagaaaca gagaggcgct ttataacggg ttaaaagagc
     6601 tcggatttga atgtgtcaga ccggaaggcg cattttatct ctttgtcaaa tctccggttg
     6661 aagatgaaaa ggcgttttgc gcagcggcaa agaaatataa catattgatc gttccgggaa
     6721 gttcgttcgg atgtccggga tatgtgcgta tcgcatattg tgtggcttac gagacaatcg
     6781 taaattcatt gccgaaattt aaagaacttg ccggagaata taaatagtaa gaatgtaaaa
     6841 agccatgtgc gggagaagag acaatgacgg tcagaaaaga acttttaaat aatatacgta
     6901 tagtggatcc gtatgttccg ggagaacagc cgcggcaaaa ggtagtgaaa ctgaatacaa
     6961 atgagaaccc ataccccccg gcgcccgaag tgaaagcggt ttttgatgaa ctgaaagagg
     7021 atgaatttcg tctttatccg gatccgacct cagggaagct tataaaagcg ctcgcggatc
     7081 aattaaaagt aggagaagat cagatttttg tgggagtcgg atcagacgat gtgctttcgc
     7141 tttgcttcct tacgtttttt aattcggaaa aaccgatatt atttcctgat attacatatt
     7201 ctttttatca ggtatgggca aatttgtaca ggattccgta tgtctgcccg aaattagatg
     7261 gaaagtttag aattgtaaaa gaagattatt acaaagtaaa cggaggaatc atttttccga
     7321 atccaaatgc cccgacggcg atttatgagg aagtggattt catcgaagac attttgcgtc
     7381 ataacagaga caatattgtg atcatagatg aagcgtatgt tgattttgca ggaaagtcag
     7441 cgatcagctt gattgaccgt tacgataatc tgatcgttac gcagacgttt tcaaagtcta
     7501 gaagtatggc gggaatgcga ataggatatg cagtttccaa tccggaattg atccgttgtt
     7561 taaatgatgt aaaatattcg tttaattctt atacaatgag ctgtgcggca atctcatgtg
     7621 gagcggcagc tttggaaaat acggaatatt ttaaaacaaa tataagtaaa atagtacaaa
     7681 cgcgggaaaa tgtaaaagag caactcagag atctcggatt tgaatgtaca gattcaaagg
     7741 caaatttttt gtttgtgact catccgagat atgatgcgct taaattgttt gaagaattaa
     7801 aagaagcggg aatttatgtg cggcattgga atgatcctaa gataaaagat tatcttagaa
     7861 tcacaatagg cacagatgag gagatgaaga tatttcttga tttcctgaaa aagaaaatag
     7921 gagcatagaa aatgataaaa aaattgttac aaggtatggt tgtcggaatt gcgaatatta
     7981 ttcccggagt gagcggggga acaatgatgg tcgcaatggg actttacgat aaactgattc
     8041 atgcgatcac gcatctgaaa tcagaattta agaacagcat gaaactgctt attccgattt
     8101 tcataggagc gggagcggca atcgttattc tttcaaggtt gtttgagttt cttttggaaa
     8161 attatccgat tccgacaaat tttgctttct gcggattgat cgcgggcagt cttccgttta
     8221 tttttaataa agtgaaaggt cactcggttt caacagggaa gatcgccgca tttttgatct
     8281 ttttcggaat agtgatactg atggctttgc ttggggatac aaacggaaaa gcggcagatg
     8341 taagtttcgg aattataaat atgatcaagt tgttcagcgt tggtatcatt gcggctgcaa
     8401 cgatggtcgt tcccggagta agcggatcta tgatgcttat gcttctcgga tattatgata
     8461 cgattctgaa aacaatcaat caatttattg acgctgttgt agcttttgat ataaatggta
     8521 ttttgacgca gtgtaaagtt ttgataccgt tcggaatcgg gatcgtgatc gggatcttcc
     8581 tgattgcaaa gctgatcgaa tttatttttt caaaagcgga gatccat
//
LOCUS       DBMDXF010000073         8490 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2237_length_8490_cov_1.638455, whole genome shotgun sequence.
ACCESSION   DBMDXF010000073 DBMDXF010000000
VERSION     DBMDXF010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_8490_cov_1.638455"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..4502)
                     /locus_tag="ACZPD4_04360"
     CDS             complement(<1..4502)
                     /locus_tag="ACZPD4_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1378337.1"
                     /translation="MKKRGRALALLLAAVLTVSSVPGTVMAADGVKPQDGTTKEQPFW
                     SGTGGSTRFRIPCLVSLDDGTLVAGCDARWNTSLDGGGLDTIVSRSTDKGKTWHYTFA
                     NYLGDNGNTHNNNSTAFIDPAMATDGEKVYMIADLYPAGYALNGASHAPVAGKSHDEN
                     GNILLADARKWQNCWVNDRQNEANYTYHLEKNDKKESDSAYVIKDANGAIVEGYTVDA
                     YFNIKGKDVDANLFEADSPFQVWPTDYLYLTTSEDAGATWSVPTIVNMRKDHEQSLLV
                     GPGRGMVTSKGRILFTAYEFTGSDKNSVAIYSDDGGKTWERGKSVSGWSSEAVVTEAD
                     GKLYMFTRHGGYYTSDDFGETWSTQKNMGISYWLNCQLTAITYPKKIDGKTAILFATP
                     SSSSGRAAGKIFVGLVQDDGTLDWKYNYSINGSAYYAYSCLTILPDGTIGLLYESDGT
                     VITYEDFDIEDVAKGAAIGNIWCTDENGTVADVTMTSDMSKKLTVNGLKDGAKVEVSS
                     DNENAVTAAYADGEVTLTSKTVTGMEKATVTVESEGEKTTVDVIVTDEKDYEIVDLRV
                     GDTKTYTDKTGNYSDSTLDGLNKDIADVTLTGEDAQAVEKQVKAQLATSEAHFDGAEK
                     SLDECLFTFASVEGQDNTYTMSAKDGDKTVYVNYRSAASAGTVCAEASANIKLEERSD
                     DQTFELLDQTAGANGNRLYFHKDNESKLCFDRNTGDHANCRMELYKKAANSSAESAIP
                     GYEKVTGLSQITSGEKYLIAAKAATGTYYVVNPSAAGEKYKHVAKVVEETIPVKQEAA
                     VALGSNAQFNDRGEKKISKCLFTFDKQAEEGKYKISATTEDGQKVYLGPKSAASAQTP
                     LTTTEAVITVAKTDKGFTLNQMENSANGGYLYFWKDNEGKFHFDRNSSVDPNGKCEFE
                     LYKKADNAEAAEIPGYEKLNDMSEIEANGQYLIAMKAKDNKYYLLNPATGNDKYSYVA
                     KVTGEMYKDETIGAKTDIAITGKSEGKTSVKIGNKTYFIFVKNDVEEVTIKIGETYNV
                     PGKILNESEVTKDGIVSLEKRENMPPYKAISEIAEGTYLFGNNSHIMLNTESTAAGSE
                     KGLGMKAVNFNNDETAKDFMWTLTKSGEGYTMKDVKSGKYINISGNNVELKDAEQVLT
                     IRARANGGFSVSANNSYLNNWAGNNNKVAAYPSDDNGWSFYKASAGNVVTGVKEGTVT
                     VAASEGTNYKITVVKDVPEVKEYTVTATVNDEAMGTAKLSPEADKYKEGTEVTATAKV
                     KDENKYEFVNWTVGNEEVSKKAEYKFTVDKDMELKANFKAKEEPQPPVEKTYKVTATV
                     NDETMGTAKLSPEADKYKEGTEVTATAKVKDESKYEFVNWTVGNEEVSKKAEYKFTVD
                     KDMELKANFKAKDPVEETFTVKVKANDTNMGEAKLDPAKAEYAKGEKVKAIATAKEGH
                     EFVNWTVDGKAVSAEATYEFLVEKNVELTANFKETEKPVEQFTVTVKANDDKMGTVAI
                     DPVKEN"
     gene            complement(4813..>4910)
                     /locus_tag="ACZPD4_04365"
     CDS             complement(4813..>4910)
                     /locus_tag="ACZPD4_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR1378338.1"
                     /translation="LGQQENPEGLGWATGDNYGYDIVRMIHDMKK"
     assembly_gap    4911..4920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4921..5654)
                     /locus_tag="ACZPD4_04370"
     CDS             complement(<4921..5654)
                     /locus_tag="ACZPD4_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR1378339.1"
                     /translation="MARQNERKMKLYMEAGLAVLLVLTIALASVSGRLSSQAHEKDKN
                     KQEAAVQGEAEVYEKNKGNDQEKNEVKDQRENNAQNTAGNAEESQETIADGQYPIMGN
                     SRVTVDEMVDYFQTAGKPYPKEALSKGGAESIEVFCRMYYDEAVAEGVRPEVAFVQTM
                     KETGFLQYGGDASVEQFNFAGLGTTGGGVPGNSYPDVRTGIRAQIQHLKAYATADPLN
                     GKCVDDRYEYVKKGTAPYVEWLGTLAL"
     gene            complement(5792..6538)
                     /locus_tag="ACZPD4_04375"
     CDS             complement(5792..6538)
                     /locus_tag="ACZPD4_04375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1378340.1"
                     /translation="MRLKDKVAIITGGSRGIGFATADAFLREGAKVAVAASSEKNAEA
                     AVEKLKAIHPDAEVVGISPNLGSFESVKEEFDKVEKQFGRIDILVNNAGVSESTPFTS
                     YTEADFDRVIDLNVKGVFNATRAVVDGMIKNHSGVVLNTSSMVSISGQPSGFAYPASK
                     FAVNGTTISLARELGPKGIRVNAVAPGITETDMMKAVPKNVIEPMIAQIPLRRLGQPE
                     DIANAFVFLASDEASYITGVVLSVDGMARA"
     gene            complement(6760..7695)
                     /locus_tag="ACZPD4_04380"
     CDS             complement(6760..7695)
                     /locus_tag="ACZPD4_04380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR1378341.1"
                     /translation="MYFLGIDGGGTKTDFLLLDENGKTIAQRKIGTISYKHVGMDYVT
                     ELLRENINQILDGNDAYICLAFPNWGESRVNDERFLCRIDKITDRPMKIVNDSAAGWA
                     GSLGLEEGINLVAGTGSIAYGQNKFGTEARAGGWDDGFSDEGSCYWLGKKALELFSKE
                     SDGRVKKGKLLEIFRYNFNLKNDFDLIDIFDEVYKNNRTKTAELQKLLLQAAMQGDEE
                     AIRLYEAAAEELALIIGAVYRKLEFSGDTIVSYSGGLFHAGDFILKPLVRKLEENGIC
                     LCPPKYSPVQGAALLAAREYSRDGEFLSRIKAGLE"
     gene            complement(7695..>8490)
                     /locus_tag="ACZPD4_04385"
     CDS             complement(7695..>8490)
                     /locus_tag="ACZPD4_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR1378342.1"
                     /translation="NMELLKTCYETMEKNVFDPWFKEIYENTWSPVFIWHDMYLTCLS
                     TTETCAEVLGTDVTKKLLNECVDKIANWFANDEYNVVFEAVTRENKIDIESDKGRFIN
                     PGHMHESAWFLMKAGVLLEDKQITMRGIQIARWANAVGKDEEYGGIISYADALGETPR
                     PIDWFKETNSLWNEKVWWPNAEALTAYAQAYCYTKDSYFLQEFLDQHNYCKEKFFDSQ
                     YKEWFERLEYDGTVKNSAKGTPWKCAFHLVRALVFTLEAFSEMEAV"
BASE COUNT         2056 a         2184 c         1316 g         2924 t
ORIGIN      
        1 taattttctt tcaccggatc gatcgctaca gttcccatct tgtcgtcatt tgcttttact
       61 gttactgtaa actgctctac cggtttttct gtctctttga agttagctgt cagttctaca
      121 ttcttttcta ctaagaattc atatgtagct tctgcagaaa ctgcttttcc gtccactgtc
      181 cagtttacaa actcgtgtcc ttctttagct gtagctatgg cttttacttt ctctccctta
      241 gcatattctg cttttgcagg atcaagtttc gcctctccca tattagtgtc atttgctttt
      301 acttttactg taaatgtctc ttctacagga tcttttgctt tgaagttcgc cttaagctcc
      361 atgtctttat caactgtaaa cttgtactct gctttcttag aaacttcttc attgcctact
      421 gtccagttta cgaactcata cttgctttcg tcttttactt tcgcagttgc tgttacttct
      481 gttccttctt tgtacttatc agcttccgga ctaagctttg ccgtacccat cgtttcatca
      541 tttactgttg ccgttacttt gtatgtcttt tctacaggcg gctgcggctc ttcttttgct
      601 ttgaagttcg ccttaagttc catgtctttg tctactgtaa acttatactc tgctttctta
      661 gaaacttctt cattgcctac tgtccagttt acgaactcat atttgttttc gtcttttact
      721 ttcgcagttg ccgttacttc tgttccttct ttgtacttat cagcttccgg gctaagcttt
      781 gccgtaccca tcgcttcatc gtttactgtt gccgttactg tgtactcttt tacttcagga
      841 acgtctttta caaccgtaat cttatagttt gttccttctg atgccgctac agttactgtt
      901 ccttctttca cacctgttac tacgtttccg gcagatgctt tgtagaagct ccatccgtta
      961 tcatcactcg gataagctgc aactttatta ttatttcctg cccagttgtt cagataagaa
     1021 ttatttgccg aaacagagaa tcctccgttt gctcttgctc tgatcgtcaa tacctgctct
     1081 gcatccttca gttccacatt atttccggaa atattaatgt atttgcctga cttaacatct
     1141 ttcatcgtat agccttcgcc tgatttggta agcgtccaca tgaaatcttt cgcagtctca
     1201 tcgttattga agttgacagc tttcattcca agaccctttt ccgaacctgc tgctgtactt
     1261 tccgtattca gcataatatg actattattt ccgaacaaat atgttccttc tgcgatttcc
     1321 gaaattgctt tgtaaggcgg catgttctca cgtttttcca aacttacgat accgtctttt
     1381 gtcacttcgg attcatttaa aatcttgccc ggtacgttat atgtctcgcc gatcttgatt
     1441 gttacttctt ctacatcatt tttcacgaag atgaagtatg tcttgttacc gatcttcaca
     1501 gatgttttac cttctgattt tccggtgatc gcaatatctg ttttcgctcc gatcgtttcg
     1561 tctttgtaca tttcgcctgt cacttttgca acatagctgt acttatcatt tcctgtcgca
     1621 ggattcaaca gataatactt attatctttc gctttcatag cgatcaaata ctgaccgttt
     1681 gcctcgattt ctgacatgtc attcagcttc tcgtaacccg gtatttcagc tgcttctgca
     1741 ttatccgctt ttttataaag ctcaaattcg cattttccat tcggatctac actgctattt
     1801 ctgtcaaagt ggaatttacc ttcattatct ttccagaagt acaaatatcc gccgtttgca
     1861 gaattttcca tctgattcaa tgtgaatcct ttgtccgttt ttgcaactgt gatcaccgcc
     1921 tctgttgttg taagaggagt ctgtgctgaa gctgcagatt tcggtccgag atatactttc
     1981 tgtccatctt ctgttgttgc agaaatttta tactttcctt cttctgcctg tttatcgaat
     2041 gtaaacagac atttactgat ttttttctcg cctctgtcat taaactgtgc attgcttccg
     2101 agcgctacgg cagcttcctg tttcaccgga atcgtttcct caactacttt tgcaacatgt
     2161 ttatactttt ctccggcagc cgacggattt acaacataat aagttcctgt agctgctttt
     2221 gccgcgatca aatatttttc gccgcttgtg atctgcgata accctgtcac tttctcatat
     2281 cccggaattg cagactctgc actgctgttt gcagcctttt tatacaattc catgcgacag
     2341 ttcgcgtgat cgcctgtgtt tctgtcgaag cacagtttac tttcattatc cttatggaaa
     2401 tacagacggt ttccgttagc acctgcagtc tgatccaata attcaaacgt ctgatcgtcg
     2461 cttctttcct ctaacttaat attcgctgat gcttccgcac atactgtacc ggctgaagcc
     2521 gcacttctgt aatttacata tactgttttg tctccatcct tagcagacat tgtatatgtg
     2581 ttatcttgtc cttctacact tgcaaatgta aacaaacatt catccaaaga cttctctgct
     2641 ccgtcaaaat gtgcttcact tgttgcaagc tgtgctttta cctgtttctc aactgcttgt
     2701 gcatcttctc ctgtcaatgt cacatctgca atatctttat taagaccgtc taatgtgctg
     2761 tcactgtaat ttcctgtctt gtctgtatat gttttcgtat ctcctacacg aagatctacg
     2821 atctcataat ctttttcatc tgttacgatt acatcgactg tcgtcttttc accttcagac
     2881 tcaactgtta cagtcgcttt ttccattcct gttactgttt tagatgtcaa tgtcacttcg
     2941 ccgtcagcat atgctgctgt tactgcgttt tcattgtcag aagaaacttc cacttttgcg
     3001 ccatctttta agccgtttac tgtcagtttt ttcgacatat cagatgtcat tgtaacatct
     3061 gccacagtac cgttttcgtc tgtgcaccag atatttccga ttgctgcgcc ttttgcaacg
     3121 tcttcaatat caaaatcttc atacgtaatc acagttccgt cactctcata aagaagaccg
     3181 atcgttccgt caggaagaat tgtcagacaa gagtatgcgt aatatgcaga tccgttaatg
     3241 ctgtagttat atttccagtc aagtgttccg tcatcctgca ctaagcctac gaaaatcttt
     3301 cccgccgcac gtcccgatga agacgatgga gttgcaaaca agattgctgt tttaccatca
     3361 attttcttcg gatatgtaat tgctgtcaac tgacaattca gccagtaact aatccccata
     3421 tttttttgcg ttgaccatgt ttctccaaaa tcatccgatg tataatatcc gccatggcga
     3481 gtaaacatat acaattttcc atctgcctca gttacaactg cttctgaact ccatccagat
     3541 acagactttc ctctttccca cgtcttaccg ccatcatccg aataaatagc cacgctattt
     3601 ttatctgaac ctgtaaattc atatgccgta aataagatcc tgcctttaga agtaaccata
     3661 cctcgtcccg gtcctactaa aagagactgc tcatgatctt ttctcatatt gacaatcgta
     3721 ggtacagacc acgttgctcc tgcatcttcc gatgttgtca ggtacaaata atctgtcggc
     3781 catacctgaa acggcgaatc agcttcaaac agattcgcat ctacatcttt ccctttaata
     3841 ttaaaataag cgtcaactgt ataaccttct acgattgcgc cattcgcatc tttaatcaca
     3901 tatgcagaat cagattcttt cttatcattc ttttccaaat gatatgtata gttagcttca
     3961 ttctgacgat catttaccca acaattctgc catttccttg cgtctgccag aagaatattt
     4021 ccgttctcat catgactttt acctgcgacc ggtgcatgac tcgcgccatt cagcgcatat
     4081 cctgccggat aaaggtctgc aatcatgtac accttttctc cgtccgttgc catggcagga
     4141 tcgataaacg ctgtagaatt gttattatgc gtgttcccgt tatcaccaag gtaattagca
     4201 aacgtatagt gccatgtctt tcctttgtct gtcgaacggg atacgatcgt atccagtcca
     4261 ccaccgtcta aactcgtatt ccatctggca tcgcagccgg ctaccaatgt gccgtcatca
     4321 agagatacaa gacacggaat tctaaacctt gtacttcctc ccgttccact ccagaatggc
     4381 tgttctttcg ttgttccgtc ttggggcttc acgccatctg ccgccataac tgttcccggc
     4441 acactactga ccgttaaaac cgcggcaagc agaagcgcta aagcccttcc tctttttttc
     4501 atactttttc cctccgttta ttttcaggct catgtcaatc cccccgaagt gccaaaaggc
     4561 atattccggg attgtaagcg atcgccaagt tctcggcgaa ctcagacttt ggcttatcgc
     4621 cattacaaaa aagaaaaacc gtattaaacc atctacgatt ttccatttta caccttattt
     4681 taaattatac tattcgccat attgtttttc aatgagattg cccttgacaa aaccactgaa
     4741 tttttcgtgc taaacaacag tgtttccctt caattttttg tgtattttac acaattttag
     4801 ctaacaatat ttctatttct tcatatcgtg gatcattcgg acgatatcat atccgtagtt
     4861 atctcccgtt gcccagccaa gcccttcagg attttcctgc tgccccagcc nnnnnnnnnn
     4921 agggcaagtg tccccagcca ttccacatat ggagccgttc ctttttttac atactcatac
     4981 cgatcatcta cacacttccc attcaaaggg tctgctgtgg catatgcttt cagatgttga
     5041 atctgcgccc tgatcccggt ccgcacatca ggataagagt tcccgggcac acccccgcct
     5101 gttgtaccta atcccgcaaa attaaactgt tcgacagaag catctccgcc atactgcaaa
     5161 aatcccgttt ctttcatcgt ctgcacaaaa gcaacttccg gacgaacgcc ttctgccacc
     5221 gcttcatcat aatacatcct gcaaaacact tctatagact ctgctccacc tttggaaaga
     5281 gcttctttcg gataaggttt tcctgccgtc tgaaaataat ctaccatctc atcaactgtt
     5341 acgcggctgt ttcccataat cggatactgt ccgtcagcga tcgtttcctg tgattcttca
     5401 gcatttcccg ctgtattttg agcgttgttt tccctttggt ctttcacttc atttttctct
     5461 tgatcatttc ctttgttttt ttcgtaaact tccgcttctc cttgtacagc cgcttcctgc
     5521 ttattcttgt ccttctcatg tgcctgcgaa gagagccgcc ctgatactga tgccagtgca
     5581 atcgttaaaa caagaagtac cgccagaccc gcttccatat ataatttcat tttccgctca
     5641 ttttgacgcg ccatatattc accatcccag aaatttaaaa tatctcaacg gaacaacttc
     5701 cgctgagata ttatattagc acatgttcaa atatatgaaa aggaatccct tcccggtaaa
     5761 ttattttgca aacaatattg caacgccata tttacgcgcg cgccattcca tctacactaa
     5821 gaactacgcc tgtaatatag gacgcttcat cagatgcaag aaatacaaat gcatttgcaa
     5881 tgtcttccgg ctgtccgagt ctgcgaagcg gaatctgtgc gatcatcggc tcgatcacgt
     5941 ttttcggaac ggctttcatc atgtctgttt ccgtaattcc cggagcaact gcattgacac
     6001 ggattccttt tggcccaagc tctcttgcca gagagatcgt cgtaccgttt accgcaaact
     6061 tagacgccgg ataagcaaat ccgctcggct gaccggaaat acttaccatt gaagacgtat
     6121 tcaggacaac cccgctgtga tttttaatca ttccgtcaac taccgcgcgt gttgcgttaa
     6181 atacaccttt tacattcaga tcgatcacac gatcaaaatc agcttctgta taagatgtaa
     6241 acggagtgct ctcggaaacc cccgcattat tcaccaaaat atcaatacgt ccgaactgtt
     6301 tctctacttt atcaaattct tctttcacac tttcaaaact tccgaggttc ggactgatcc
     6361 ctaccacttc tgcatccgga tgaatcgctt ttaacttctc gactgccgct tccgcgtttt
     6421 tttcactgct cgccgcaaca gcaactttcg caccctctct taaaaatgca tctgctgtcg
     6481 caaatccgat tccacggctt cctcctgtaa taatcgctac tttatctttt aatctcataa
     6541 atcgttcctc ctgtttttct cttgttttta tgtgtcgatc ttactctctt ataattttca
     6601 ttgcacatta tataagtata ataacacata atagaaatta tgtctgtgat tttatcacca
     6661 aataaaaata tccggcaaaa taattataaa cataatataa aataaaagag aatatctcgc
     6721 agcaaaaaat cgcaatctgc cgcggatatt ctctttttct tattctaagc ctgcttttat
     6781 tcgtgataaa aattcaccgt ctctgctata ttctctcgcc gccaaaagcg ccgctccctg
     6841 tacaggcgaa tatttaggag gacaaagaca aattccattt tcctcgagct tccgcaccag
     6901 tggctttaat ataaaatctc ctgcatgaaa caatccacct gaataagata cgatcgtgtc
     6961 tcccgaaaat tccagttttc tataaacagc gcctatgatc agcgctaatt cttctgccgc
     7021 agcctcatac aaacgtattg cttcttcatc tccttgcatc gctgcctgta aaagcaattt
     7081 ttgaagttca gctgtttttg tacgattatt tttatacact tcatcaaaaa tgtctataag
     7141 gtcaaagtca ttttttaaat taaaattgta tcggaaaatc tcgagtaact ttcctttttt
     7201 tacccttccg tcactctctt ttgaaaacag ctccaacgct ttctttccca accaataaca
     7261 agagccttca tcagaaaaac catcatccca tccgcccgca cgagcttctg tgccaaactt
     7321 attttgtcca taagcgatcg aacctgttcc tgccacaaga ttgatcccct cttctaatcc
     7381 caaagatcct gcccatcccg cggcgctgtc gtttacaatt ttcatcggcc tatctgtaat
     7441 tttgtcaatc cggcataaaa agcgttcatc atttactctt gattctcccc aattcggaaa
     7501 ggcaagacat atataggcat cattcccgtc taatatttga tttatatttt ctctcagtaa
     7561 ttctgtcaca taatccattc caacgtgctt ataagaaata gttcctatct tgcgctgtgc
     7621 aatcgttttt ccgttttcat ctaacagtaa aaaatcagtt tttgttcccc ctccgtcaat
     7681 ccccagaaaa tacactatac cgcctccatt tccgaaaatg cttctaatgt aaacacaagc
     7741 gctctcacaa gatgaaacgc acatttccac ggtgttcctt ttgcactgtt ttttacggtt
     7801 ccgtcatatt ccagtcgttc aaaccactct ttatattgtg aatcaaaaaa cttttcttta
     7861 caataattat gctgatctaa aaattcttga agaaaataac tgtctttcgt atagcaatac
     7921 gcctgtgcat aagctgtcaa cgcctctgca ttcggccacc atactttttc attccataaa
     7981 gagttcgttt ctttaaacca gtcgatcggg cgaggtgttt cccccaatgc gtctgcatat
     8041 gaaataattc caccatactc ttcatctttt ccgacggcat ttgcccaacg ggcaatctgt
     8101 ataccgcgca tagtaatctg cttgtcttcc agcaatactc ctgccttcat aagaaaccat
     8161 gctgattcat gcatatgtcc cggattaata aagcggcctt tgtctgactc aatatcaatt
     8221 ttattttctc ttgtaaccgc ttcaaacact acgttatatt catcatttgc aaaccagtta
     8281 gcaatcttat cgacacattc attcaaaagt ttttttgtaa catccgtccc caatacttcc
     8341 gcacatgttt ccgttgtaga cagacatgtc agatacatgt catgccatat aaaaaccggg
     8401 ctccatgtat tttcatatat ttctttaaac catggatcaa atacattttt ttccatcgtt
     8461 tcataacaag tttttaaaag ttccatattt
//
LOCUS       DBMDXF010000074         8358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2279_length_8358_cov_2.592588, whole genome shotgun sequence.
ACCESSION   DBMDXF010000074 DBMDXF010000000
VERSION     DBMDXF010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2279_length_8358_cov_2.592588"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(640..4374)
                     /gene="rpoC"
                     /locus_tag="ACZPD4_04390"
     CDS             complement(640..4374)
                     /gene="rpoC"
                     /locus_tag="ACZPD4_04390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1378343.1"
                     /translation="MPNNNEQQYQPLTFDAIKIGLASPEKIMEWSRGEVTKPETINYR
                     TLKPEKDGLFCERIFGPSKDWECHCGKYKKIRYKGVVCDRCGVEVTKASVRRERMGHI
                     ALAAPVSHIWYFKGIPSRMGLILDISPRTLERVLYFASYIVLDKGETDLQYKQILSET
                     EYQEQKEKWGSGSFRVGMGAEAIKELLESIDLEKEYAELQAGLENATGQKRARIVKRL
                     EVVEAFRESGNRPEWMILSAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRIINRNNRL
                     KRLLELGAPDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRALKSLSDMLKGKSGR
                     FRQNLLGKRVDYSGRSVIVVGPELKIYQCGLPKEMAIELFKPFVMKELVANGTAHNIK
                     NAKKMVERLQPEVWDVLEEVIKEHPVMLNRAPTLHRLGIQAFEPILVEGKAIKLHPLV
                     CTAYNADFDGDQMAVHVPLSQEAQAECRFLLLSPNNLLKPSDGGPVAVPSQDMVLGIY
                     YLTQERPGVKGEGMFFKSMSEAILAYENGYITLHSRIKVRVTRMMPDGTEKTGTIDAT
                     LGRLLFNEIIPQDLGFVDREVEGNELLLEVDFHVGKKQLKQILEKVINTHGATQTAEV
                     LDNVKAIGYKFSTRAAMTVSISDMTVPPQKPQMIEEAQNTVDKITKNYKRGLITEEER
                     YKEVVETWKATDDKLTEALLTGLDKYNNIFMMADSGARGSDKQIKQLAGMRGLMADTT
                     GRTIELPIKSNFREGLDVLEYFMSAHGARKGLSDTALRTADSGYLTRRLVDVSQELII
                     HDSDCAEEGKEIPGMYVSAFMDGNEEIESLQERITGRYSCEDIKDKDGNVLVKANHMI
                     TPRRAERVMKKGVDENGESLKKVKIRTILTCKCKNGVCAKCYGANMATGEAVQVGEAV
                     GIVAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVEELFEARKPKGLAIITEFAGR
                     AMISDTKKKREIIVTNDETGESKAYLIPYGSRIKIQDGVMLEAGDELTEGSVNPHDIL
                     KIKGLRAVQDYMLQEVQRVYRLQGVEINDKHIEVIVRQMLKKVRIEEKGDTEFLPGTM
                     VDVLEFNEVNERLEEEGKEPAIGEQIMLGITKASLATDSFLSAASFQETTKVLTEAAI
                     KGKVDHLVGLKENVIIGKHIPAGTGMKKYRDVALNTDARAEAMLAEELETEESAEVEN
                     AEAVSADAVNESLENEETALTDEVMDTEEADDEEAFVTEE"
     gene            complement(4392..8255)
                     /gene="rpoB"
                     /locus_tag="ACZPD4_04395"
     CDS             complement(4392..8255)
                     /gene="rpoB"
                     /locus_tag="ACZPD4_04395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1378344.1"
                     /translation="MEKNRIRPIKTGKSFRMSYSRQKEVLEMPNLIEVQKDSYQWFLD
                     EGLKEVFDDISPIADYSGHLSLEFVDFTLCEDDVKYTIDECKERDATYAAPLKVRVRL
                     HNKETDEINEHEIFMGDLPLMTKTGTFVINGAERVIVSQLVRSPGIYYGIAHDKLGKK
                     LYSSTVIPNRGAWLEYETDSNDVFYVRVDRTRKVPITVLIRALGIGTNAEIVDLFGEE
                     PKILASFTKDTAESYQEGLLELYKKIRPGEPLAVDSAESLINSMFFDPRRYDLAKVGR
                     YKFNKKLMLKNRIAGCILAEDAVSQLTGEIVAEKGTKITRELADKIQNNAVPYLWVEG
                     EDEERNIKILSNMMVDLQAVTDIDPEEVGVTEQVYYPVLAGIIEESAGDIEEMKALIK
                     RDIHDLIPKHITKEDILASINYNMHLEYGMGTDDDIDHLGNRRIRAVGELLQNQYRIG
                     LSRLERVVRERMTTQDQEGISPQSLINIKPVTAAVKEFFGSSQLSQFMDQNNPLGELT
                     HKRRLSALGPGGLSRDRAGFEVRDVHYSHYGRMCPIETPEGPNIGLINSLATYARINQ
                     YGFVEAPYRWIDKSDPENPVVTEKVVYMTADEEDNYHVAQANTPLDEEGHFIHKNVSG
                     RYREETQEYERSKFDYMDVSPKMVFSVATALIPFLENDDANRALMGSNMQRQAVPLLT
                     TEAPVVGTGMEVKAAVDSGVCVVAERAGTVESSTSKEIVVREEDGKKTSYKLTKFQRS
                     NQSNCYNQRPIVNKGDVVAAGQVIADGPSTSGGEMALGKNPLIGFMTWEGYNYEDAVL
                     LSERLVQDDVYTSVHIEEYEAEARDTKLGPEEITRDIPGVGDDALKDLDERGIIRIGA
                     EVRAGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGEYGIVVDAKV
                     FTRENGDELSPGVNQAVRIYIAQKRKISVGDKMAGRHGNKGVVSRVLPVEDMPFLPNG
                     RPLDIVLNPLGVPSRMNIGQVLEIHLSLAAKALGFNISTPVFDGANEIDIMDTLDLAN
                     DYVNLEWEEFEKKHGQELLPEVLQYLSDNREHRNLWKGVPLSRDGKVRLRDGRTGEYF
                     DSPVTIGHMHYLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALE
                     AYGASYTLQEILTVKSDDVVGRVKTYEAIIKGENIPEPGIPESFKVLLKELQSLALDV
                     RVLRDDNTEVEIMENVDYGETDLRHIIEGDRKYRDENESFGDHGFSEKEFVGEELEDV
                     EPEEEPDDDAPVDIEFDEYSDYEEE"
BASE COUNT         2002 a         2201 c         1608 g         2547 t
ORIGIN      
        1 tttattgcaa ataaaggaac aattccagca cctggaagag tggaaaagac tgataatcac
       61 cacggagaaa gatgcggcaa ggttgaaact ccatcccgca cagcctcaat acatttttac
      121 cgaagtcgga gtcggctatc gtatggctga agatgaagat gagtaaatgt taaaaacccg
      181 ctaaggtttt tgccgcttta ttccatttta cactttgctc ctttaaaata gagactgatt
      241 tgtttttatt tcatttgttt tgcacagaaa attatttttt tgtgtaaaac cgcaaaattt
      301 attttaagga gtgacctaca atgaatatcg gtttaatttt acttatgcta atcggaggct
      361 ctgtcggagt tttttctaca ctctacattc tgatcagcct tcctgtcata atttttctga
      421 agatttatcg aaaaattcga ttcggagctt ctctttatga ttaaataaaa tcaatgattt
      481 tcttatctta tgcatgtttt ttacagagct gaacaaaaaa ctccgtatac gtcaccgggt
      541 tttatcccga atctgtatac ggagttttta ttttatctta ttctttgtct ttcttgttta
      601 ttattcttgc actttcttgc ttagcagaac aatgatcttt tattcttctg tgacaaaagc
      661 ctcttcatca tctgcttctt ctgtatccat cacttcatcc gtcaacgctg tctcttcgtt
      721 ttccaaactt tcatttactg catctgcgct tactgcctca gcgttttcca cttctgcgct
      781 ctcttcagtt tcaagttcct ctgccagcat cgcttctgct ctggcatctg tatttaacgc
      841 tacgtcacga tattttttca tacccgtacc cgccggaata tgtttaccga taattacgtt
      901 ttctttcaga ccgacaagat gatcgacttt tcctttgatt gccgcttcgg taagaacttt
      961 cgttgtttcc tggaaagatg cggctgaaag gaaggaatct gtagcaagtg aagctttcgt
     1021 aataccaagc atgatctgct cgcctatagc cggctctttt ccttcttctt caagacgctc
     1081 gtttacttca ttgaattcaa gaacgtcaac cattgttccc ggaaggaatt ctgtatcacc
     1141 cttctcttcg atgcggactt tcttcaacat ctggcgaacg ataacctcga tatgcttatc
     1201 gttgatctca acaccctgca gacggtatac tctctgcact tcctgaagca tataatcctg
     1261 aacggctcta agacctttga tcttcagaat atcatgcgga tttacactac cttccgtcag
     1321 ttcgtctccg gcttcaagca tcacgccgtc ctgtatttta atacgagaac cgtacgggat
     1381 caaatacgcc tttgattcgc ctgtctcatc atttgtaacg atgatctcac gtttcttctt
     1441 cgtgtcggaa atcattgcgc gtccggcaaa ttccgtgatg atcgcaagac ctttcggctt
     1501 tctcgcctcg aaaagctcct cgacacgcgg aagaccctgt gtgatatcgt caccggcaac
     1561 tcctcctgta tggaacgtac gcattgtaag ctgtgttccc ggctcaccga tcgactgggc
     1621 agccacaata ccaactgctt ctccgacctg aaccgcttct cctgtcgcca tgtttgctcc
     1681 gtaacatttt gcacatacgc cgttcttaca tttacatgta aggatcgtac ggatcttcac
     1741 tttcttcagt gactcgccgt tctcatccac gccttttttc ataacgcgtt ccgcgcggcg
     1801 cggtgtgatc atgtggtttg cttttacgag tacatttccg tctttatctt tgatgtcctc
     1861 acaggaatag cgtcctgtaa tacgctcctg aaggctctca atctcttcat ttccatccat
     1921 aaatgcagat acatacattc ccggaatctc ttttccttct tctgcacaat cagaatcatg
     1981 gataatcaat tcctgagata cgtctacaag acgtctcgtc aggtaacctg aatcggctgt
     2041 acgaagagcc gtatctgaca gacctttacg agcaccatga gcagacatga aatattcaag
     2101 tacgtcaaga ccttcacgga agtttgattt gataggcaac tcgatcgtac gtcctgtcgt
     2161 atctgccata agtccacgca tacctgcaag ctgcttgatc tgcttatcag atccacgggc
     2221 tccagagtca gccatcataa agatgttatt atatttatca agacctgtca gcagagcctc
     2281 tgtcagctta tcatcggttg ctttccaagt ttcgacaact tctttataac gctcttcttc
     2341 tgttataaga ccacgtttgt aattctttgt gattttatct acggtattct gtgcttcctc
     2401 gatcatctga ggtttctgcg gcggcaccgt catatctgaa attgatacag tcatagccgc
     2461 tcttgtagag aatttgtatc cgattgcctt tacattgtca agcacttccg ctgtttgtgt
     2521 tgctccgtga gtattgatta ctttttcaag aatctgtttc aactgtttct ttccgacatg
     2581 gaaatcaacc tcgagaagca gctcgtttcc ttctacctct cgatctacga atccaagatc
     2641 ctgcggaata atctcattaa acaaaagacg tccgagtgtt gcatcgatcg ttcctgtctt
     2701 ctcagttccg tccggcatca ttctagttac acgaactttg attcgcgagt ggagcgtaat
     2761 atatccgttc tcatatgcaa gaattgcttc gctcatgctc ttaaagaaca ttccttcgcc
     2821 ctttactccc ggtctttcct gcgtcagata ataaataccg agaaccatat cctgtgaagg
     2881 aaccgccacc ggtcctccgt cggacggttt cagcaggttg ttcggtgaaa gaagcaagaa
     2941 acggcactct gcctgagctt cctgtgacag cggaacgtgt accgccatct ggtcaccgtc
     3001 gaagtcggca ttgtacgctg tacaaacgag cggatggagc ttgattgcct taccttctac
     3061 aagaatcggc tcaaatgcct ggataccgag tctgtgaagc gtgggggcgc ggttaagcat
     3121 aaccggatgt tccttgatca cttcctcaag aacatcccaa acttccggct gaagtctctc
     3181 taccattttc tttgcatttt ttatattatg tgctgttccg tttgccacaa gttctttcat
     3241 aacaaacggt ttaaacagtt cgattgccat ctctttcgga agaccgcact gataaatctt
     3301 aagttccggt cctacgacga taacggaacg tcccgaatag tcgacacgtt ttccaagcag
     3361 gttctgacgg aaacgtccgg atttaccttt cagcatatct gaaagtgatt tcagcgctct
     3421 gtttcccggg cctgtaaccg gacgtccacg gcgtccgttg tcgatcaaag cgtctaccgc
     3481 ttcttgaagc atacgttttt cgtttcttac gataatgtcc ggcgctccaa gctcaagcag
     3541 tcttttcaga cgattgtttc tgttaatgat tcttctgtac agatcattca aatcacttgt
     3601 cgcaaaacgg cctccgtcaa gctgtaccat aggacgaaga tccggcggaa taaccggaat
     3661 tgcagaaagg atcatccatt ccggacggtt gcccgactca cggaacgcct caacaacttc
     3721 aaggcgtttc acgattctcg ctctcttttg acctgtcgca ttttccaacc ctgcctgaag
     3781 ttctgcatat tctttttcaa gatcgatgct ttccagaagt tctttgattg cctcggctcc
     3841 catgcctacg cggaatgagc cgctgcccca cttctctttc tgttcctgat actctgtctc
     3901 agagagaatc tgtttatact gaagatccgt ctctcctttg tcgagaacaa tgtatgaagc
     3961 aaagtaaagc actctttcca atgttctcgg agagatatca aggatcaatc ccatacggct
     4021 cggaattccc ttgaaatacc agatatgaga caccggagcg gcaagggcga tatgccccat
     4081 acgctcacga cgcacacttg cctttgtaac ctcgacgccg catcggtcac aaacaacacc
     4141 tttgtaacgg atctttttat attttccaca atgacactcc cagtctttgc tcggtccgaa
     4201 gattctctca cagaacagtc cgtctttttc cggttttaat gttctgtagt tgatcgtctc
     4261 cggctttgta acctcacctc ttgaccattc catgatcttt tccggtgaag ccagaccgat
     4321 cttgatcgcg tcaaatgtta acggttgata ttgttgttca ttattgtttg gcatatattt
     4381 ggatcctcct cttattcttc ctcataatcg gaatactcgt caaactcaat gtccaccggg
     4441 gcatcatcat ccggctcttc ctcaggttca acatcctcga gttcctcgcc tacaaactcc
     4501 ttctcactaa atccgtgatc accgaaagat tcattctcat cacggtactt tctgtctcct
     4561 tcgataatgt gacgcagatc tgtctcgccg taatctacgt tctccataat ctctacttct
     4621 gtattgtcat cacgcaggac acgcacatcc aacgcaagag actgaagctc ttttaaaagc
     4681 accttaaatg actccggaat acccggttcc ggtatatttt ctcctttgat gatcgcctcg
     4741 taagtcttca cacgacctac aacgtcgtcg gacttcactg tcaggatctc ctgcagtgta
     4801 taagatgcgc cgtacgcctc cagcgcccaa acttccatct ctccgaaacg ctgtccgccg
     4861 aactgtgctt taccaccgag cggctgctgc gtaacgaggg agtaaggacc tgtagaacgc
     4921 gcatggatct tatcatctac aagatggtga agtttcaggt agtgcatgtg tccgattgtt
     4981 accggactgt cgaaatactc acctgtacgt ccgtctctca aacgcacttt accgtctctt
     5041 gagagcggaa cgcctttcca aaggtttctg tgttctctat tgtcagacag gtactgcaat
     5101 acttccggaa gaagctcctg tccatgcttt ttctcaaact cttcccactc aagattgaca
     5161 taatcatttg ccagatcaag agtatccata atgtcgatct cgtttgctcc gtcaaagaca
     5221 ggcgtagaaa tgttaaatcc aagagctttc gccgcaagac tcaagtggat ctcaagcacc
     5281 tgtccgatat tcatacgaga cggcacacca agcggattca aaacaatatc cagcgggcgc
     5341 ccgttcggaa gaaacggcat atcctctacc ggaagcacac gggaaacgac acccttgtta
     5401 ccgtgacgtc cggccatctt atctcctaca gatatctttc tcttctgcgc gatgtaaatt
     5461 ctgaccgcct gatttacccc gggtgaaagc tcgtcgccgt tttctcttgt aaatactttc
     5521 gcatctacaa cgataccata ctcaccgtgc ggcactttca aggatgtatc tcttacttca
     5581 cgggctttct ctccgaaaat cgcacggaga agtctctcct cagccgtaag ctctgtctct
     5641 cctttcggag ttacttttcc gacgagaata tctccggcgc gcacttccgc accgatacgg
     5701 atgattcctc tctcatcaag gtctttcaac gcatcatcgc cgacgcccgg aatatctctc
     5761 gtgatctctt caggtccgag ttttgtatct cttgcctctg cctcatattc ttcgatatga
     5821 acagaagtat aaacatcatc ctgtacaagt ctctcactca gaagaacagc atcctcgtag
     5881 ttataacctt cccatgtcat aaaaccgatg agcgggttct taccaagagc catctctccg
     5941 cctgatgtag acggtccatc tgcaataacc tgtcctgccg caacaacgtc gcctttatta
     6001 acgatcggac gctgattgta acagttactc tggttgcttc gctggaattt tgtcagctta
     6061 taagatgtct tcttgccgtc ttcttcgcgg acaacgatct cttttgacgt agagctttct
     6121 actgttcccg cccgttctgc gacaacacat actcccgagt caacggcagc ttttacttcc
     6181 atacccgtac cgactaccgg agcctctgtc gtcagaagag gaactgcctg acgctgcatg
     6241 ttcgatccca taagagcacg gttggcatcg tcgttttcaa ggaacggaat cagagccgta
     6301 gcgactgaga ataccatctt cggagaaaca tccatgtaat cgaacttgct tctctcgtat
     6361 tcctgtgttt cctctctgta acgaccggat acatttttat gaataaagtg gccttcttca
     6421 tcaagcggtg tattcgcctg tgccacatga taattgtctt cttcgtcagc tgtcatataa
     6481 acaacttttt ctgtaacgac cggattttcc ggatcagact tatcaatcca acgatatggc
     6541 gcttcaacaa atccatactg attaattcgc gcatatgttg ccaaagagtt gatcagaccg
     6601 atgttcggac cttcaggcgt ctcgatcgga cacattcttc catagtgtga ataatgtacg
     6661 tctcgaacct cgaatccggc gcgatctctg gaaagacctc ccggtccaag tgcggaaaga
     6721 cgtctcttat gtgtcagctc tccaagcggg ttgttctgat ccataaactg agacaactga
     6781 gaagaaccga agaactcttt caccgctgcc gtcaccggtt tgatattgat cagtgactgc
     6841 ggggaaattc cttcctgatc ctgtgtcgtc atacgctcac ggacaactct ttctaatctt
     6901 gaaagaccga ttctgtactg attctggagc aattctccta ctgcgcggat acggcggtta
     6961 cccaaatggt caatgtcatc atccgttccc atgccatatt caagatgcat attatagttg
     7021 atggacgcga ggatgtcctc ttttgtaata tgcttcggga tcaaatcgtg aatgtcacgt
     7081 ttgatcaacg ctttcatctc ttcgatgtct cctgcggact cttcgataat tcccgccaaa
     7141 accggataat atacctgctc cgtaacacct acttcttcag gatcgatatc tgtaacagcc
     7201 tgaagatcta ccatcatatt agaaaggatc ttgatgtttc tctcttcatc ttcaccttct
     7261 acccacagat aaggaacggc attgttctgg atcttatctg caagttcacg agtaatcttc
     7321 gtaccttttt cagcaactat ctctcccgta agctgactca ccgcatcctc tgcaagtatg
     7381 cagcctgcaa tacggttttt cagcatcagt ttcttattga atttataacg tccaaccttt
     7441 gcgagatcat atcttctcgg atcaaagaac atgctgttga tcagactttc cgcgctgtct
     7501 actgcaagcg gctcgcccgg gcggatcttc ttgtacagtt ccaaaagacc ttcctgataa
     7561 ctctccgcag tgtcctttgt aaagcttgca aggatcttcg gctcttcacc gaaaagatca
     7621 acaatctctg cattcgttcc gataccaagt gcacggatca gcactgtgat cggaactttt
     7681 cttgtacgat cgacgcgcac ataaaaaacg tcgttggagt ctgtctcata ttcaagccat
     7741 gcgcctcggt ttgggatcac tgtagatgag tacagtttct ttccgagttt atcatgagca
     7801 atgccataat aaattcccgg agaacgcacg agctgactaa cgataacacg ctctgcaccg
     7861 ttgataacaa atgtacccgt ctttgtcatc agcggaagat ctcccataaa aatctcatgc
     7921 tcgttaatct catcagtctc tttattgtgg agacggactc tcacttttaa gggagcagca
     7981 taagtcgcat cgcgctcttt acactcatcg atcgtatatt tcacatcgtc ctcgcaaagt
     8041 gtgaaatcta cgaattccag acttaagtgt ccgctgtaat cagcgatcgg ggaaatatcg
     8101 tcgaacacct ctttgagtcc ttcgtcaaga aaccattgat aggaatcttt ttgaacttca
     8161 atgaggttag gcatctccaa tacttctttc tgccgtgagt aactcatgcg gaaacttttt
     8221 ccggttttaa tgggacggat tctgtttttc tccattgacg tttcactcct ctatcatttt
     8281 ttcaatcatt cctctttcgt tgtccgcatc tcctttgtcg gacaacatcg aatgacctga
     8341 atctttacaa acgggact
//
LOCUS       DBMDXF010000075         8353 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2283_length_8353_cov_1.810955, whole genome shotgun sequence.
ACCESSION   DBMDXF010000075 DBMDXF010000000
VERSION     DBMDXF010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2283_length_8353_cov_1.810955"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="asnA"
                     /locus_tag="ACZPD4_04400"
     CDS             complement(<1..1066)
                     /gene="asnA"
                     /locus_tag="ACZPD4_04400"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1378345.1"
                     /translation="MEHLKIPEGYSSPLTIRETEVAIKEVKDHFERALAKSLHLTRVS
                     APLFVLPQSGMNDNLNGIERPVSFGIKEQDDACAEIVHSLAKWKRYALKKYGFHSGEG
                     LYTDMTAIRRDEDTDNIHSLYVDQWDWEKIISKEERNMETLEYTVRKVYSALKDTEDY
                     ISRRYNYIEPLLPDDIFFITSQELEDMFPDCTPKERELRIAKAKGAVFISQIGKVLAS
                     GEKHDGRAPDYDDWELNGDIIVYYPVLDIALELSSMGIRVDEESLKSQLKTAGCEDRA
                     KLPFQKSLLDGELPYTVGGGIGQSRICMYYLRKAHIGEVHSSMWPESIVETASENGIH
                     HPRQTENAHREQRLLPYPAEH"
     gene            1880..2050
                     /locus_tag="ACZPD4_04405"
     CDS             1880..2050
                     /locus_tag="ACZPD4_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378346.1"
                     /translation="MKRKRKTAREIFIVICLFLTIFLAVLFAFGRTEIPAVVYMSVCT
                     ACLFGGVAAIQE"
     gene            2212..3027
                     /locus_tag="ACZPD4_04410"
     CDS             2212..3027
                     /locus_tag="ACZPD4_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR1378347.1"
                     /translation="MKIDMHCHVKEGSIDSKVGVEEYITILKKHGFQGMVITDHDTYN
                     GYRYWKENIKGKKHTDFVVLKGIEYDTRDAGHILVIMPEGVKMRLLEMRGMPLALLID
                     LVHRNGGVLGPAHPCGEKYMSFTNARRYYLSPEIVKRFDFVEAFNSCEPVSSNSGAEK
                     LAKKYGKVMTGGSDSHKPDCVGRGYTILPEPVTCETELISLIHKKTPFKAGGVLYNKT
                     TKEKIGKINKLLVYSFWVYNKSGELVKRHGRNKKMEEEHPFDPIDPIELYYHS"
     gene            complement(3088..3435)
                     /locus_tag="ACZPD4_04415"
     CDS             complement(3088..3435)
                     /locus_tag="ACZPD4_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378348.1"
                     /translation="MLKYIPYVLMFAVATMIIYGWGLWRTMHQKQDLSNMLCSKGISK
                     VKKALKKNKSLTKKELEPFVKDLTAKQPFNSERIGVTDPNAFLDSILPYMLKQKMIKE
                     IREDKKVVYQLNK"
     gene            complement(3435..3797)
                     /locus_tag="ACZPD4_04420"
     CDS             complement(3435..3797)
                     /locus_tag="ACZPD4_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR1378349.1"
                     /translation="MSKKSKKNEKNYLDFIPVKNPDIEYETDENGIVTVFIEWKGFYH
                     KIAQKIFRRPRVSDIKMDKYGSFVWHAIDDEKDVHQLSKEMESEFPKMEKSLSRLIKF
                     LEIMKDNNLIYWKGEEKK"
     gene            complement(3935..5827)
                     /locus_tag="ACZPD4_04425"
     CDS             complement(3935..5827)
                     /locus_tag="ACZPD4_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR1378350.1"
                     /translation="MEKKNEFKPYIPADKIVPEFTVTSIVIGIILAVVFGAANAYLGL
                     IVGMTVSASIPAAVISMGIIRVILRKDSILENNLVQTIGSAGESVAAGAIFTLPALFI
                     WAEEGKIAFPSMMTIFLVALFGGVLGICFMVPLRQALIVEEHGTLPFPEGTACAEVLL
                     AGEEGGAKAGTVFAGLGIAAAYKFIADGFKLFPSEIGYAFKSYAGSQIGIQVLPALAG
                     VGFICGPKISSYMLAGGTLSWFVLMPAIALFGADATIFPGTAPISELLAANGPSALWS
                     NYIKYIGAGAVATGGMISLIKSFPLIVRTFKQAMSSMSKKRSNTTLVRTQQDLPMPIL
                     LVVLLVIVVAIWLIPAFPVSPLGSLIIVIFGFFFATVSSRMVGLIGSSNNPVSGMAIA
                     TLIIATLALKATGTTGTKGMVGAIAIGGIICVIAAIAGDTSQDLKTGFIVGATPKKQQ
                     IGELIGVIVSAAAIGGVLYLLNEAWSYGSKELPAAQATMMKMLVEGIMNAELPWGLIL
                     IGVFIAIVVEIIKVPVMPFAVGMYLPFSLSAGIMAGGAVRFLVEKIKGTDEEKKARTD
                     KGVLFTSGLIAGEGITGILLAGFAVAKLDVALKFSLPQVGSLIIFIALLGGLFALCMK
                     AKPAKK"
     gene            6142..6798
                     /locus_tag="ACZPD4_04430"
     CDS             6142..6798
                     /locus_tag="ACZPD4_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1378351.1"
                     /translation="MNEILEQALPFWESLTDKQKEEIRNNMVKTECQKKTQLHFGGGE
                     CAGVQIIGKGRVRVFITSPGGGDITLFRLLEGDVSILSAACMLNGMDVELDMEMETDC
                     ELYTIPKCVYRKLYDESGIVKDYTMEMISEKFSDIMWLFNQFVFSNVASRIASALLDH
                     KVMERSDSLAITHEALARDAGTAREVVTRILKQFQADGLVKLTRGKVEILNEAGLRKI
                     "
     gene            6955..7263
                     /gene="trxA"
                     /locus_tag="ACZPD4_04435"
     CDS             6955..7263
                     /gene="trxA"
                     /locus_tag="ACZPD4_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1378352.1"
                     /translation="MAVVKLTTANFDQEVLQADRPVLVDFYADWCGPCKMMGPVVEEI
                     SGELDDVKVCKINIDEEMEVAQRYGVMSIPTFIAFKNGEMTGKQVGAVPKGALADLVK
                     "
     gene            7367..>8353
                     /locus_tag="ACZPD4_04440"
     CDS             7367..>8353
                     /locus_tag="ACZPD4_04440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1378353.1"
                     /translation="MSLQFIMGNSGSGKSYCLYDTVVREAIENPDKNYLVIVPEQFTM
                     QTQKDLCLAHPKGGIMNIDVLSFGRLAYRVFEEVGQDARVVLDDEGKNLVLRKIAGEC
                     EKDLKVFKGNLKKQGYISEVKSVLSEFAQYGVDFERLDDFMKEVPPESYLHYKLDDIR
                     TIYEKFEEYLSEKYITKEEMLDVLSDAVPYSDILKGSVVAMDGFTGFTPVQNRLIGEL
                     FSVCEKVMITVTMDGRENPYVYEHPYQIFALGKQMVTSLMKIAGEKKTEIEEPICLFE
                     RPVYRFRDCSEMAFLETELFRYSGAKYNGSCESVSLHEVRSPGKEAKYVAESI"
BASE COUNT         2487 a         1490 c         2011 g         2365 t
ORIGIN      
        1 gatgctcagc aggatagggg agaagtcttt gctctcgatg cgcattttcc gtttgtcgag
       61 gatgatggat tccgttttcg gaagcggttt cgacgattga ttccggccac atagaagaat
      121 ggacttctcc gatgtgagct tttcttaaat aatacataca gatacgtgac tggccgatcc
      181 cgccgcctac agtataaggg agttctccgt caagaagaga tttctggaaa ggcagtttag
      241 cgcggtcttc gcatcctgcg gtttttaatt gagattttaa tgattcttca tcgacacgga
      301 tgcccataga agaaagctcg agagcgatgt caagaaccgg ataataaacg atgatatcgc
      361 cgtttaactc ccagtcgtca tagtccggcg cacgtccgtc gtgcttttcg cctgaagcga
      421 gcacttttcc gatctgtgag atgaatacgg cgccttttgc ttttgcaata cgcagttctc
      481 gttcttttgg agtgcagtca gggaacatgt cttccagttc ctgtgaagtg atgaagaaaa
      541 tatcatcagg aagaagaggt tcgatatagt tgtaccttct tgatatgtag tcctcggtat
      601 cttttaatgc actgtatacc tttctgactg tgtattccag tgtttccata ttccgttctt
      661 ctttggaaat aatcttttcc caatcccact gatcgacata aagggagtgg atgttatcgg
      721 tatcttcgtc acgacggatt gcagtcatgt cagtgtaaag accttcgccg gaatgaaaac
      781 cgtatttttt taaggcatat cgcttccatt ttgcgaggga atgaacgatt tccgcgcagg
      841 catcgtcctg ctctttgatt ccgaatgata ccggacgttc aattccgttt agattgtcgt
      901 tcataccgga ctgcggaaga acaaacaacg gggcagatac acgcgtcaaa tgaagagatt
      961 ttgcaagcgc gcgctcgaaa tgatccttaa cttctttgat agcgacttct gtttcacgga
     1021 ttgtaagcgg agaagaatat ccctccggta tttttaaatg ttccatatat aaccactcct
     1081 cttttgatgt attgacattt atgttttatt ttaatgggga gaaggggaga agtcaactgt
     1141 gacttttttg attatgttac tgacagattt tttcatggag aaaattcatt gagcttttct
     1201 ttttgctgct cgtttgctgc ctgtatttca gattattcac aatgaggata taaaataaca
     1261 gataatttaa aataaaatga aaaaatatta gaaataatat tgacaaacac ataatagagt
     1321 gatattatta aatcaacaaa taaaacaaac taaataatca atcaaaacta aataaaaaat
     1381 attttgtttt gaaagaattt tagtaaatgt tttattcatc catcggatat cgtgatcagg
     1441 gatcggatgg aaagatacag agaaagatca taatgacgag aaagttatga gagaagtaaa
     1501 atctgtattt gaaaaatcca tcggaaactg aagttcgatg gaaatctaag aataggaggt
     1561 acggaccacc gggaacgtaa gttgatctat caaaaagaag atagaaaaga gaggtaaaaa
     1621 aagaatgata gtaatatcac attgaaagtg gatgttgatc aagatgagtt gattggcatc
     1681 cactttcagt tgtgcggaaa tatattcggt aaaatagcga aagcataaaa cttggcagag
     1741 gtatgtatat tgctatgtat tgctgtatat tgcgtaatga gtcgggttta tggcacacct
     1801 tttctgataa gattttttta gaaaactttt tggaaaactt tttggaaaag atgatatttt
     1861 ggaaaggtgt gtttttatta tgaagagaaa aagaaagaca gcaagagaaa tttttatagt
     1921 gatctgttta tttttgacaa tatttctggc ggtgttgttt gcatttggga gaacagagat
     1981 tccggcagtc gtttatatgt ctgtatgtac ggcatgtctt ttcggagggg ttgcagcgat
     2041 acaggagtga gtgaccgggt gttttggatt ttatcaacta ccgttgtctt ctgaaatgtg
     2101 aaatgtcggt gtataacagt tgtaatcaac tgttcggatg atgtataata atttcatgaa
     2161 tgatagggat aaaaaatatc aggtgattac accggaaaag ggggcttgtg tatgaaaata
     2221 gatatgcatt gccacgtgaa agaaggctcg atcgacagca aggttggagt ggaagagtat
     2281 attacgattt taaaaaagca tggttttcaa gggatggtca ttaccgatca tgacacatac
     2341 aacggatacc gatattggaa agaaaatata aaaggaaaga agcatacgga ttttgttgta
     2401 ttgaaaggga ttgagtatga cacaagggat gccgggcata tccttgttat tatgccggaa
     2461 ggggtgaaga tgcgtctgct tgagatgcgg gggatgccgc tggcgctttt aatcgacctt
     2521 gtgcatcgaa acggcggagt cctaggtcct gctcatccat gcggtgagaa gtatatgagt
     2581 tttacgaatg cgcggcggta ttacttatct ccggagattg taaagcgttt tgattttgta
     2641 gaggcattta atagctgtga gcccgtttcg tcgaattcag gtgcggaaaa actggcgaaa
     2701 aaatatggaa aagttatgac tggcggaagt gattcacata agccggactg tgtcggacgg
     2761 ggatatacga tccttccgga acctgttaca tgtgagacgg aactgatttc gctgatccat
     2821 aagaaaactc cgtttaaagc gggaggagtg ctttataata agactacgaa ggaaaagatc
     2881 ggaaagatca acaagctttt ggtatattcc ttctgggttt ataataagag cggagaatta
     2941 gtgaaaagac atggcagaaa taaaaaaatg gaagaagagc atccgtttga tccgatcgat
     3001 ccgatcgaac tgtattatca ttcttgatcc gatgaatgga aaatgatcgc tgtgtggagc
     3061 atgcgataag aaaaagttgt ctatcagtta tttgtttaac tgatagacaa cttttttgtc
     3121 ttctcttatt tcttttatca ttttttgttt cagcatatac gggagaatcg aatcaaggaa
     3181 agcatttgga tcggtcactc cgattcgctc gctgttgaac ggctgttttg cggtcagatc
     3241 ttttacaaag ggttccagtt ctttttttgt cagcgactta ttttttttga gcgctttttt
     3301 gactttggag attccttttg aacagagcat gttggagaga tcctgtttct ggtgcattgt
     3361 gcgccaaaga ccccatccgt aaatgatcat tgtggcaacg gcaaacataa ggacgtaggg
     3421 aatgtatttt aacattattt tttctcctct cctttccagt agatcaggtt gttgtctttc
     3481 atgatctcaa gaaatttaat taaacgggat aatgattttt ccattttcgg aaattcagat
     3541 tccatttctt tggaaagctg atgtacgtct ttttcatcgt ctatcgcatg ccatacgaaa
     3601 cttccgtact tatccatttt aatatcgctg actctcgggc ggcggaatat tttctgggca
     3661 attttgtgat agaagccttt ccattcgata aagacggtga cgattccgtt ttcatctgtt
     3721 tcatattcta tatccggatt ttttacagga ataaaatcaa gatagttttt ttcgtttttt
     3781 ttactttttt tactcatctt aatgaagatc ctctctacaa tatcattaca gaataatatc
     3841 ataaaaagag gcagcctgca agcagcagac tgcctccggt gaacaatcag atcatgtatg
     3901 tactttgttt atacgttgaa ctgaatgtac ggttttattt tttagcaggt ttcgctttca
     3961 tgcagagtgc gaacaatccg ccgagaagag cgatgaagat aatcagactt ccaacttgcg
     4021 gaagtgagaa ttttaaagct acgtcaagtt tcgcaactgc aaatcctgca agcaggatac
     4081 cggtgatacc ttctcctgcg ataagtcctg atgtgaagag cacgcctttg tctgtgcggg
     4141 ctttcttttc ttcgtccgta cctttgatct tttcaacaag gaaacgaaca gctccaccgg
     4201 ccatgatacc tgcgcttaag ctgaacggga gatacatacc gactgcgaac ggcattaccg
     4261 gaacttttat gatctcgact acgattgcga taaacacacc gataaggatg agtccccacg
     4321 gaagttctgc gttcatgata ccttcaacga gcattttcat cattgtagcc tgagctgccg
     4381 gaagttcttt tgaaccgtaa gaccaagctt cgttcagaag gtagagaact ccgccgatag
     4441 ctgccgctga aacaatgaca ccgatcaatt caccgatctg ctgtttcttc ggtgtagctc
     4501 ctacgataaa tccggtttta aggtcctgag acgtatctcc ggcgattgct gcgattacac
     4561 agatgatacc gccgatagcg attgctccga ccataccttt tgtacctgtt gtacctgttg
     4621 cttttaaagc cagtgtagcg atgatcaatg ttgcgatcgc cataccggaa acagggttgt
     4681 tagaagaacc gatcagacct accatacgag aagaaacggt tgcaaagaag aaaccgaaga
     4741 ttacgatgat aagtgatccg agcggactta cagggaatgc cgggatgagc cagattgcaa
     4801 caacgatcac aagaagaact acaagaagga tcggcatagg aagatcctgc tgtgtacgaa
     4861 cgagcgttgt gttggaacgt tttttggaca tactggacat agcctgttta aatgtacgga
     4921 cgatcagcgg gaagctcttg atcaggctga tcataccgcc ggtagcaaca gctccggctc
     4981 cgatgtattt gatgtagttg ctccaaagag cagacggtcc gttggctgca agcaattcag
     5041 agatcggagc tgtgcctggg aagatcgtag cgtcagcgcc gaacaaagcg attgccggca
     5101 taagtacgaa ccagctcaga gtacctcctg caagcatgta gcttgaaatc ttcggtccac
     5161 agatgaagcc gacacctgca agagcaggaa gaacctgaat accgatctga gatcctgcat
     5221 agcttttgaa agcataaccg atttcgcttg ggaataattt gaatccgtct gcgatgaatt
     5281 tgtaagctgc tgcgataccg agtcctgcga atacagtacc tgcttttgcg cctccctctt
     5341 ctcctgcaag aagaacttct gcgcaggctg ttccttccgg gaacggaagt gtgccgtgtt
     5401 cttcaacgat caatgcctga cgaagcggca ccatgaagca aattccgagg acacctccga
     5461 aaagtgcaac gagaaagatc gtcatcatac ttgggaaagc gatttttccc tcctcagccc
     5521 agataaataa tgcaggcaga gtgaagatag cacctgccgc tacagactct ccggcggaac
     5581 cgattgtctg cacaagattg ttttcgagaa tggaatcctt acgaagaatc acacggatga
     5641 tacccataga gataactgct gccggaatgg aagctgatac tgtcataccg acgatcagtc
     5701 cgaggtatgc gtttgcggct ccgaaaacga ctgcaaggat aataccaatc acgattgaag
     5761 ttaccgtaaa ctccggaacg attttatctg ccggaatata cggtttgaac tcatttttct
     5821 tttccatgat gttcattcct tttcctttct gtaaaattaa agactttgta gtaaagtcta
     5881 taacattata ccactttggc gtgataaaag gaagaagaaa aatttgtatt tatgcaatat
     5941 tcgtcaatat ttctacagta atattaatat ttattaatat tatgacggtt ttttttgaca
     6001 tattctgcgt tttggataaa attgcagggg aaaacggcga agaagaaatt caggcggagc
     6061 gggcggagag attttacact ttatatttca ggaagattct tttataataa gaggcagcaa
     6121 gacggaaggt gaaaagtgga aatgaatgaa attttagaac aggcgcttcc tttttgggaa
     6181 agtctgacag ataaacagaa agaagaaatc aggaacaata tggtaaaaac agaatgccaa
     6241 aagaaaacgc agctccattt cggcggcgga gaatgcgccg gagtccagat catcggaaaa
     6301 ggaagagtgc gggtatttat tacatcaccg ggcggcggag atattacatt attccgtttg
     6361 ctggaaggag atgtcagtat tctgagcgcg gcgtgtatgc ttaacggtat ggatgtggag
     6421 cttgatatgg agatggaaac agactgtgaa ctttatacga ttccaaagtg tgtgtatcgc
     6481 aaactgtatg atgagagcgg aattgtgaaa gactatacga tggagatgat atcggaaaag
     6541 ttttctgaca tcatgtggct gtttaatcag ttcgtttttt caaatgtagc atctcgtatc
     6601 gcatcggctc tgctcgacca taaagtgatg gagcgctcag acagtctggc gattacgcac
     6661 gaggcattgg cgcgggatgc ggggacagcg agggaagtag tgacaaggat actgaagcag
     6721 tttcaggctg acggacttgt gaaactgacc cgcgggaaag tggaaatact taacgaggca
     6781 ggactgagaa aaatctaatc ttgaaacggc aaaacggcac atagatgtta attgtgattt
     6841 tgtcactgaa aagaaaattc atccggtata atataaagcc atactcaatg aaagaaaaca
     6901 aaaatcgaat caataaaaac agaccgaata aggtcgggaa aaggagaaag aaaaatggca
     6961 gtagtaaaat taacaacggc aaattttgat caggaagttc ttcaggcaga tcgcccggta
     7021 ttggtagatt tctacgctga ctggtgcgga ccgtgtaaga tgatgggacc ggtagtagaa
     7081 gagatttccg gagaattgga cgatgtaaaa gtatgtaaga tcaatattga cgaagagatg
     7141 gaagtagcgc agcgctacgg tgttatgagc atcccgacat ttatcgcgtt taaaaacgga
     7201 gagatgacag gaaaacaggt cggcgctgtt ccgaagggag cgcttgcaga tctggtaaaa
     7261 taacaaaaca tagtaaagga cagataaacc ggcttcttgt caggaatgtg cacgggtagt
     7321 ataatcaaga aaaatatgtt ggaagacctg tggcaggagg cggtttatgt cattacaatt
     7381 tattatggga aattccggaa gcggaaaatc ttactgttta tatgatacgg ttgttcggga
     7441 agcgattgaa aatcctgaca agaattatct tgtcatcgtt cccgagcagt ttacgatgca
     7501 gacacaaaaa gatctctgtc ttgcgcatcc aaaaggcggg atcatgaata ttgatgtgtt
     7561 aagtttcggg cgtctggcgt accgcgtttt tgaagaggtg ggtcaggatg cccgtgttgt
     7621 gcttgatgat gaaggaaaaa atcttgtttt gcgcaaaatt gcgggcgagt gtgaaaaaga
     7681 tctgaaggtc tttaagggaa acctgaaaaa acaaggttat ataagcgaag tgaagtctgt
     7741 tctttcggaa tttgcgcagt atggcgtaga ctttgaacgt ttggatgatt ttatgaaaga
     7801 agtgccgcct gaaagttatc tgcattataa actggatgat atccgaacta tatatgaaaa
     7861 atttgaagag tatttaagtg aaaaatacat tacaaaagaa gaaatgctcg atgtgctcag
     7921 cgacgctgtt ccgtattccg atattttaaa aggaagcgtg gtggcgatgg acgggtttac
     7981 aggttttacg cctgtacaaa accgtttgat cggtgagctt ttttcagtgt gcgaaaaagt
     8041 gatgatcaca gtgacgatgg acggaagaga aaatccgtat gtatatgaac atccgtatca
     8101 gatttttgcg ctcggcaaac agatggtcac atctcttatg aagattgcgg gtgagaaaaa
     8161 aacagagatt gaagaaccga tatgtttgtt tgaaaggccg gtgtatcgtt ttcgggattg
     8221 ttcggaaatg gcgtttttgg aaacggaact attccggtat tcaggcgcaa aatataacgg
     8281 aagctgtgag tctgtatcgc tgcatgaagt gcgctctccg ggaaaagagg caaagtatgt
     8341 agcagaaagt atc
//
LOCUS       DBMDXF010000076         8344 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2284_length_8344_cov_2.213393, whole genome shotgun sequence.
ACCESSION   DBMDXF010000076 DBMDXF010000000
VERSION     DBMDXF010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2284_length_8344_cov_2.213393"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..4011
                     /locus_tag="ACZPD4_04445"
     CDS             <1..4011
                     /locus_tag="ACZPD4_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HIR1378354.1"
                     /translation="KEIEEKIKKQKEQGNAYFDEEALIEYLKQNMINYGNGINSGVST
                     GNNTLNNIKESRENVFRYLQRITDEFRSPVYADTAESRHAVTISTGQELIEKINENPN
                     GFYVLEKDISMADINLTGEVYIDKTFIGKLQGNGHKITDAQGPLFAKIANSYVSDLTI
                     VNKEGETKDWFGKTKQYTIIVNEQKKETVQEIKTLEELKTVGQNKYTKYVLKNDIDAS
                     SVTTETAVVKGIFKGEFDGGGFTIKGLKKPLFEKVQEGTVRNLKIENAEINSTEESSK
                     NAVITKESNHAVFESLNLADIKVSGVSYNAVVTGYDYTSSVFSKIQIRNAQITGTKNY
                     NAVLAGRASGSQIQDVSVIGSSVALSGTDCGGFIGEGKNVTISRVYSDADMTVNTYTD
                     DKNRTQSAGFIGNLTGKSSVEYVFAAGKVDNKTSEQLYNFIGTPDALKTMVKNSFVIQ
                     NAGGVSNITDGVGQEILREVTSQEAATSDFYKTSMTLNEETWNLSLVPMKGYPELKGM
                     EKREVISVKTAEDFMKMKDFPTQEYRLKADIDLSGTEQTGSVIPEFSGVLDGENHKIT
                     GLKAPLFGQLSGTVSNVAIDAGAIEIGNSVDTTVGIFANTMTNATVEKVMIANGSISS
                     TAGKAAGFAGTVTDSTVKNIFIQGRVNAVSTASGFAETSHHSVMENIYANIDVNGADG
                     AGFLVNSTGENSYKNICSIGNVAENMYKLAKTDITFTNAYELSAADGISSAAEANGVK
                     TIGKEVWTKAFYTETLKLDISVWDVENAETNGYPLLKEFNVNLSPMTVEIQKPQDIRK
                     LNKLPEGRFTITADLDFTEYGAAEITENIAENSIENNADINAADSVENSAENHAEETA
                     QAGTCLVTETFTGSINGGGHKVSGMKSAMFKQLSGKVENLEFRNILVDNETAGANVLA
                     ETTHNANVKNVHFNGITLRGAGYTGMIGKDTGSTFSQISVQNADVTTRADYAGVFAAN
                     AAGTQIFDVLITDTEVATSNAYVGGFIGNAERITAQKVFADAELDIPYTVSPQNTAAF
                     IGQASEDSKIQYSTAAGGVYPEDPSSTRYKLTHMDNSSNLNELKAFTNCFINTDTPGY
                     DSIANDPKGVTHEALCGTEFYTNEMRLSQDVWDLSDVAGTGTPSLKTMPEEDVRAPET
                     APTPEEEIPMQETAPEGYTEIRTAEELLAIRDSSDKYILMSSISLYDAEPQDGSFLGD
                     FKGELNGNGLTIREVYGAPLFNTLSGKVENLKLTDVKVEAWSQNQGANAFAKTLSGAT
                     VSKVALKNILVAGGNNTGALAGTAQNSTVSEVWAEGLNVNPYGPIHGQNDAMVGGLIA
                     KLKGGCHISDSYVAAR"
     gene            3975..5759
                     /locus_tag="ACZPD4_04450"
     CDS             3975..5759
                     /locus_tag="ACZPD4_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HIR1378355.1"
                     /translation="MPYQRQLCGGEITVNGETQGGVFGYNSNMDNGPENTVKQVVSNM
                     KTKAISGQTDGAGFIGMIGYDYSWSTWMKNSIAIGEAGVSSDHGSVGQAYRFATKGNS
                     ESPIRFGLQNCYEANVSGRSSVVSGYLDETGRYKETSFYQDTLKFDSSKWNFTSVEKK
                     GHPTLSWVADAEPLPPLPEGSAVTTHKQLKTEVPQGYTAIRTPADFMKIAENPSGKYI
                     LMNNISLEQVKLAEGQTSYIMKRFEGELDGNNQVIHGLRASLFDSISGTSSKKAVVKN
                     LRVQNVFVNAGYKDIFDWQGKPVLAEANGLAREVSNGRLETIYMNCVKLNGGGNTGAL
                     GGLVNETYIGKVWLEGIDINSGLSEEELGRFNLVGGAVGSLSGYNSKFEDSYVEGKII
                     MDNNQQGGVAGQVKAAVVRNVISNVEARSNKPDSWTEKSGFVGDVNTSLQYDNRWYLD
                     RCISIGNSGNNYKFLGKDLKAVTKNNLNLCYEVTGKTGRSNVTDATSQSGTLLTIDNI
                     NNVELYRDTLSFNDNTAQADPNAWDFSSVAEKGYPTLTWLLTYDGAAATMVEQTPVQD
                     QEQEAAQDENLAEAEVNLPEQDEYLKED"
     gene            5789..5941
                     /locus_tag="ACZPD4_04455"
     CDS             5789..5941
                     /locus_tag="ACZPD4_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378356.1"
                     /translation="MEVKEKYETPMMEMIEFNREEVVTMSGNGGTEPQQGDNFTDDSI
                     MGSWGE"
     regulatory      6085..6160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6375..7571
                     /locus_tag="ACZPD4_04460"
     CDS             6375..7571
                     /locus_tag="ACZPD4_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1378357.1"
                     /translation="MLISIAMMFLVGMSMGWICKKIHLPSLFGMIVTGIVLGPYVLDV
                     IDASILNISSDLRRIALIIILMRAGLSLDLNDLKKVGRPAVLMCFVPACFEIGGMVLL
                     APKLLGISILDAAIMGTVIAAVSPAVIVPKMLKLMEEGYGVKKSIPQMILAGASVDDV
                     FVIVMFTAFTGLAQGDQVSAVDFINIPISIAVGALAGFLIGFLSAEYFKKIHIRDTSK
                     VIILVCVSFLLVTFEDEFASVIPFASLIAVMTMGIALQQKRKNVAKRLSAKFNKLWVG
                     AEVMLFVLVGATVDIRYAVSAGVVSIVLIFAVLVFRMLGVFLCLLKTKLTMKERMFCV
                     IAYMPKATVQAAIGGVPLAMGLSCGNIVLTVAVTAILITAPLGAFLIEATYKKWLGRQ
                     TEILSR"
     gene            7652..7912
                     /gene="spoIIID"
                     /locus_tag="ACZPD4_04465"
     CDS             7652..7912
                     /gene="spoIIID"
                     /locus_tag="ACZPD4_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR1378358.1"
                     /translation="MKDYIEERAVEIAAYIIDNNATVRQTAKQFGISKSTVHKDVTER
                     LIQINPALAKEARKVLDTNKSERHIRGGMATREKYLHKMLSE"
     gene            8028..8282
                     /locus_tag="ACZPD4_04470"
     CDS             8028..8282
                     /locus_tag="ACZPD4_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378359.1"
                     /translation="MRIVIVDSEETSREVIKQAINTVVETHKIKSGCKIVGEAADGRK
                     GCEIIVKERPDLIIMDLELPGKNGLPLLKKIRSEQNTTIA"
BASE COUNT         2748 a         1450 c         2188 g         1958 t
ORIGIN      
        1 aaagagatcg aagaaaagat caaaaagcag aaagaacagg gcaacgcata ttttgacgag
       61 gaagcgctga tcgaatattt gaaacaaaat atgatcaatt acggaaatgg tattaactcc
      121 ggcgtttcaa ccggaaacaa tacgctcaac aatattaaag aatcaagaga aaacgtattc
      181 cgttatctgc aaagaattac ggatgagttc agaagtccgg tttacgcaga tacggcagag
      241 tcaagacatg cggtaacgat ttccacaggg caggagctga tcgagaagat aaacgagaac
      301 ccgaacggat tctatgttct tgaaaaggac atttctatgg cggacattaa tctgacagga
      361 gaagtttata ttgacaagac gtttatcgga aaactgcagg ggaacggtca taagattaca
      421 gacgcgcaag gaccgctctt tgcaaagatc gccaactctt acgtttccga tcttacgatc
      481 gtaaataaag aaggcgagac aaaggactgg ttcggaaaga caaaacagta tacgatcatt
      541 gtcaatgagc agaaaaaaga aaccgttcag gaaattaaga ctttagaaga attaaaaaca
      601 gtcggacaga acaaatatac aaaatatgtg ctgaaaaacg atattgacgc gtcatccgta
      661 acgacagaga cggcggttgt caaaggcatc ttcaaaggag agttcgacgg aggcggattc
      721 acgatcaaag gactgaaaaa accattgttt gaaaaagttc aggaaggaac agtccgcaac
      781 ttgaagatcg aaaatgcaga gatcaacagt acagaagaaa gcagcaaaaa cgcagttatc
      841 acaaaagaat caaaccacgc tgtctttgaa agcctgaacc tggcggatat caaagtcagc
      901 ggagtgagct acaatgcggt agtgacagga tatgattata caagctctgt tttctcgaaa
      961 atacagatcc gcaatgcgca gattacaggt acgaaaaatt ataacgccgt tttggcaggc
     1021 cgcgcatccg ggtcgcagat tcaggatgtc tctgtaatcg gaagcagtgt tgcacttagc
     1081 ggaacagact gcggcggatt catcggagaa ggcaaaaacg tgacgatcag tcgcgtgtac
     1141 agcgatgcgg atatgaccgt taatacatac acagacgata agaaccgtac gcagagcgcc
     1201 ggattcatcg gcaatcttac aggcaagagc agcgtagaat atgtatttgc ggcaggaaaa
     1261 gtagacaata aaacaagcga acagctttat aacttcatcg gtacgccgga cgcgctgaaa
     1321 acgatggtga aaaacagttt tgttatccaa aacgcaggcg gagtatcgaa tatcacagac
     1381 ggcgtaggac aggagatctt gcgtgaagta acgtctcaag aggcggcaac gtcagatttc
     1441 tacaaaacat caatgactct gaacgaagag acatggaact tgagtttagt tccgatgaaa
     1501 ggttatccgg aactgaaagg aatggagaag cgagaggtca tctccgtaaa aacggcggag
     1561 gacttcatga agatgaaaga cttcccgact caggaatacc gtctgaaagc agacatcgat
     1621 ctgtcaggaa cagaacagac aggaagcgtg ataccggaat tttccggtgt gctcgacgga
     1681 gagaatcata agatcaccgg tctgaaagcg ccgctgttcg gtcagttaag cggtacggtg
     1741 tcgaatgtag ccattgatgc aggtgcgatt gaaatcggaa acagcgtaga tacgactgtc
     1801 ggtatatttg cgaatacgat gacaaacgca actgttgaaa aagtcatgat cgcaaacgga
     1861 agcatcagca gtacagcagg aaaagcggcg ggattcgcag gaactgtgac agactcaact
     1921 gtgaaaaata ttttcattca aggaagagta aatgcagttt cgacagcgtc cggtttcgca
     1981 gaaacgtccc accattcggt tatggaaaat atctatgcaa acatagacgt aaacggggca
     2041 gacggcgcag gattccttgt gaacagtaca ggtgaaaata gttacaaaaa tatctgttca
     2101 ataggaaacg tcgcagaaaa catgtataaa ctggcaaaaa cagacatcac gtttacaaat
     2161 gcttatgagt tgtcggcagc agacggaatc agctctgcgg cagaagcaaa cggcgtgaaa
     2221 acgatcggaa aagaagtgtg gacgaaagcg ttctatacag aaacacttaa gctagatata
     2281 tccgtatggg atgtagagaa tgcagagacg aacggatatc cgttgttgaa agagttcaat
     2341 gtgaacttat ctccgatgac tgtggaaatt caaaaaccgc aggatatccg gaagctgaac
     2401 aaactgccgg aaggaagatt tacgatcacg gcagatctgg atttcacaga gtacggcgcg
     2461 gcagaaatta cagaaaacat tgcggaaaac agcatagaaa acaacgcaga tataaacgcg
     2521 gctgacagcg tggaaaacag cgcggaaaat catgcggaag aaacagcgca ggcaggaacg
     2581 tgccttgtga cagagacgtt tacaggaagt ataaacggag gcggccacaa agtaagcggc
     2641 atgaagagcg cgatgttcaa acagctttca ggaaaagtag aaaaccttga atttagaaat
     2701 attcttgtgg ataatgaaac agcaggagca aatgtgcttg cggaaacgac gcacaatgca
     2761 aatgtgaaaa acgtacattt caacggaatt acactgcgag gcgcagggta cacaggtatg
     2821 attggaaaag atacaggatc gacatttagt cagatcagtg tacagaacgc agacgtgacg
     2881 acaagagccg attatgcagg tgtgttcgcg gcaaatgcgg caggaactca aattttcgat
     2941 gtgctgatca cagatacaga agtggcaacg tcgaacgctt atgtgggagg attcatagga
     3001 aacgctgaga gaatcaccgc acagaaagta ttcgcagatg cagagctgga tattccgtat
     3061 actgtttccc cgcaaaatac ggcggcgttt atcggtcagg catcggaaga cagcaagatt
     3121 caatatagta cggcggcagg cggcgtctat ccggaagatc cgtcgtcaac aagatataag
     3181 ctgacgcata tggataacag ctctaatctg aatgaactaa aggcatttac aaattgcttt
     3241 attaacacag atacgccggg atacgacagc atagcaaacg atccgaaagg tgtgacgcac
     3301 gaagcgcttt gcggtacaga gttctataca aatgagatgc gcttgtctca ggacgtatgg
     3361 gatctgtcgg atgtggcagg aacaggaacg ccaagcttga agacgatgcc ggaagaagac
     3421 gtgagagcgc cggagacagc gccgacgccg gaagaagaaa tacctatgca ggaaacagct
     3481 ccggaaggat atacagagat ccgaacggca gaagaactgc tcgcgatccg agacagttcg
     3541 gataaatata ttttaatgag ctcgatttca ctttacgatg cagagccgca ggacggctca
     3601 tttctcggcg acttcaaagg agaactgaac ggaaacggac tgacgatccg agaagtttac
     3661 ggagcgccgt tgtttaacac attgtcagga aaagttgaaa atctgaaact gacagacgtt
     3721 aaagtagaag cttggagtca gaaccaagga gcaaatgcat ttgcgaaaac attaagcggc
     3781 gcgacagttt caaaagtagc gctcaaaaac atcctcgtcg caggaggtaa taatacagga
     3841 gcgcttgcag gaacagcaca gaattcaacc gtaagcgaag tgtgggcgga aggattaaat
     3901 gtcaatccgt atggaccgat acatggacag aatgatgcga tggttggcgg cttgattgcc
     3961 aaattgaaag gtggatgcca tatcagcgac agctatgtgg cggcgagata actgtaaatg
     4021 gcgagacaca gggcggtgta ttcggatata atagtaatat ggataatgga cctgaaaata
     4081 cagtgaaaca agttgtttct aacatgaaga caaaagcaat ttccggtcag acagacggag
     4141 caggattcat aggaatgatt ggatatgatt attcgtggag cacatggatg aaaaacagca
     4201 ttgcaatcgg tgaagcggga gtaagcagtg atcatggaag tgtcggtcag gcttatcgtt
     4261 ttgcgacaaa agggaactca gaatcgccga taagatttgg tttgcagaac tgttatgaag
     4321 cgaatgtcag tggaagaagc agtgtagttt caggttactt ggatgagacg ggtaggtata
     4381 aagaaacatc gttctatcag gatactctga aattcgacag ctcaaaatgg aactttacat
     4441 ctgttgagaa aaaaggccat ccgacattaa gctgggtagc agacgcagaa ccgcttccgc
     4501 cattgccgga aggttcggca gtaacgacac ataaacagtt gaagacagaa gtgccgcaag
     4561 gatatacggc aatccgtact ccggcagact ttatgaagat cgcagagaat ccgagcggaa
     4621 aatacatttt gatgaataat atttcactgg agcaggtaaa actcgcagaa ggtcagacgt
     4681 cctatattat gaagcgcttt gaaggagaac tggatggcaa taatcaggtg atccacgggt
     4741 taagagcatc gctgtttgat tccatcagcg gaacgtcaag caaaaaagca gtcgtgaaaa
     4801 atctgcgtgt gcagaatgta tttgtgaatg cagggtacaa ggatattttt gattggcaag
     4861 gaaaaccagt acttgcagag gcaaacggac tcgcacgtga agttagcaac ggaaggttag
     4921 aaactatata tatgaactgc gtgaagctaa acggcggagg aaatacaggc gctttgggtg
     4981 gtttggtaaa tgaaacctat atcggaaaag tttggttgga aggaattgat atcaacagtg
     5041 gacttagcga agaagaactt ggaagattta atcttgtcgg aggagcagtc ggaagtttat
     5101 caggttataa ttccaaattt gaagattcct acgtagaagg taagattatt atggataata
     5161 atcagcaggg tggagttgcc ggacaagtaa aagcggcagt tgtgagaaat gtgataagta
     5221 atgtggaagc gagatccaat aaaccggatt cgtggacaga aaaaagcgga tttgtaggag
     5281 atgttaatac aagtctccaa tatgataatc gttggtatct tgacagatgt atttccatag
     5341 gaaattcagg aaacaattat aagtttttag gcaaagatct taaggctgtt acaaaaaata
     5401 atttgaattt atgttatgaa gttacaggaa aaacaggaag gtctaacgtt acagatgcaa
     5461 cgagtcaaag cggtacactg ctgacaatag ataatatcaa taatgtagag ttgtatcgtg
     5521 atacgctttc ttttaatgac aatacggcac aggcagatcc gaatgcatgg gatttcagtt
     5581 ctgttgcgga aaaaggctat ccgacgttaa catggctctt gacatatgac ggcgcagcgg
     5641 ctactatggt agagcagaca cctgtacaag accaggagca ggaagcagcg caggatgaaa
     5701 atcttgctga agcggaagta aacttaccgg agcaggatga atatttaaaa gaagactaaa
     5761 agatatatag aaaagaagat gaggaggtat ggaagtgaaa gaaaaatacg aaactccgat
     5821 gatggagatg atcgaattca acagagaaga agtagttact atgtcgggta atggaggtac
     5881 tgaaccccaa caaggtgata attttacaga tgatagtata atgggaagtt ggggagagta
     5941 attagtctga tatgaaacat taagagatcc ccggcaggcg ttgtcagcag gcgcgcctgc
     6001 cgggggcttt tctgcctttt actgtctttt gtcagggaaa agcgtttgag aaaatagttg
     6061 acaaaagcag taactttgat acaatttgac tatgggaatg aagttctccc ttagtgatga
     6121 aactaaaacc gctgatttgg ctgatgactt ctgcattttg acgcaggaat tatcagcttt
     6181 tttattctat aggaaatttg cattttccat agaataaaac tcttccgaag aatgcacacg
     6241 ccgcaataag gaagtttacc gctttgcggc gtttcggagg tggcaaagcg tcaaagtgtt
     6301 catgagtgag gagaggcggt ggcgaagtgg acttctttat tttattccgg tttgtcagag
     6361 aaggaggatg ggttatgctg atcagtattg ccatgatgtt tttagtcggt atgtctatgg
     6421 ggtggatctg taaaaagatt catctgccga gtttgttcgg aatgattgta acgggaattg
     6481 tacttgggcc atatgtgttg gacgtgatcg acgcttctat tttgaatatt tcatcggatc
     6541 taagaaggat cgctcttatt attattttaa tgagagcggg attgtcatta gatcttaatg
     6601 atctgaaaaa ggtgggacgt cctgctgttt taatgtgttt tgttccggca tgttttgaaa
     6661 taggaggaat ggtcttatta gcgcctaagc tgcttggaat ttcgattttg gatgccgcta
     6721 ttatgggaac ggtcattgcc gcggtttctc cggcagttat tgtgccgaaa atgttgaaac
     6781 tgatggagga aggatacggt gtaaagaaga gcatccctca gatgattctt gcaggagcat
     6841 cggttgacga tgtgtttgtg atcgtaatgt ttacggcatt tacaggatta gcgcagggcg
     6901 atcaagtttc ggcagtggac tttatcaata ttccgatttc gattgcagtc ggagctttag
     6961 caggatttct tatcggattt ctctctgcag aatattttaa aaagattcat ataagagata
     7021 cttcaaaagt tattattctt gtgtgtgttt cctttttgtt agttacattt gaagatgaat
     7081 ttgcatctgt cattccattc gcatctttaa tcgcggttat gacaatggga attgctttgc
     7141 agcagaagag aaaaaacgtg gcgaagcgat tgtcggcaaa attcaataaa ctttgggtcg
     7201 gcgcagaagt gatgcttttc gtacttgtcg gagcaactgt ggatatccgg tatgcagtgt
     7261 cggcgggagt tgtatctatt gttttgattt ttgcggtttt ggtatttcgg atgttgggag
     7321 tattcttatg tttgctgaaa acgaagctta caatgaaaga acggatgttt tgcgtgatcg
     7381 cgtatatgcc gaaagccact gttcaggcag ctatcggcgg cgtaccgctt gcgatgggat
     7441 tgtcctgcgg taatattgtg ctgacggttg cggtaactgc gattttgatt acagcaccgc
     7501 tgggggcatt tctgattgaa gcgacttata aaaaatggtt aggacgacag acagagatcc
     7561 taagtagatg atataaagga aataacagtt caatacatac ctctgcagct tgcggcatac
     7621 attgtaaaaa cgatgtttac aggggaatat tttgaaagat tatatcgaag aaagagccgt
     7681 tgagattgca gcttatatta tagataataa tgcaacggtg cggcagacag caaaacagtt
     7741 tgggatcagt aaaagtacgg tacataaaga tgtaaccgaa cgactcatac agatcaaccc
     7801 ggctttagca aaagaggcgc gaaaggtatt ggatacgaat aagtcagaac gtcatattcg
     7861 gggagggatg gctacgcggg aaaaatattt gcacaaaatg ttatctgaat agaagtgaaa
     7921 gttctttaca aatcgagtca gagccgaata tactagaaag atacagtaag gtacgaatta
     7981 gcagtaagag ataaacgcga aggtttatat ttaggcggag gtgacgaatg aggatcgtaa
     8041 tagttgacag cgaagagaca tccagagaag tcattaaaca agctataaac acagtggttg
     8101 agacacataa aataaaatca gggtgcaaaa ttgtcgggga agcggcagac ggacggaaag
     8161 ggtgtgagat aatcgtgaaa gaacgcccgg atcttattat catggatctt gaactgccgg
     8221 ggaaaaacgg tttgccactt ttgaaaaaga tacggtctga gcaaaataca actattgcat
     8281 agacttgaat gaggctatgg tgaagaaggg cgagaatgtc cgtctgacat tgaaccagaa
     8341 agcc
//
LOCUS       DBMDXF010000077         8320 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2294_length_8320_cov_1.676238, whole genome shotgun sequence.
ACCESSION   DBMDXF010000077 DBMDXF010000000
VERSION     DBMDXF010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2294_length_8320_cov_1.676238"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(102..3449)
                     /gene="mfd"
                     /locus_tag="ACZPD4_04475"
     CDS             complement(102..3449)
                     /gene="mfd"
                     /locus_tag="ACZPD4_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1378360.1"
                     /translation="MKALLAPLAELADYEEIRKNRTKENGMIQISGCVNTQKIHLLSG
                     IGSGCGYKLVVFSNEEKAKKAYEEYLLFGEETYLYPARDLLFYYADIKGKTLTNRRME
                     VLRAIAEKKKEEPVTVITTMDAFLDGIISPDEIQKNRIHITGEDTVDLTKLEQDLTAL
                     GYERESQIEAPGQFAVRGGIIDVFPLAEEMPVRIELWGDEIDSIRMFDAKSQRSIENI
                     SEITIYPASENCFGNNGLVSFLKYFPENETLLFYDEPHRLQETAETVEAEYTESLKNR
                     ADAGMKEEGEEELRVFQTKDIISEMNRYSGIGLTTLESKCGLFKVRSVYTVQAKGVNP
                     YNNSFELLTRDLKRLKRNGYRVVLLSGSRTRAKRLAEDLRDYDLSSFYSEDMQREVKP
                     GEIMAAYGYASEGYEYPMLKFVVISESDIFGRKKKKKKRKKYEGQRIQDFAELKPGDY
                     VVHENHGIGVYKGIEKIEVEKIVKDYMKIVYAEGGVLYIPVAQMDLIQKYAGADAKKP
                     RLNKLGTIQWGKTKSQVKKAVQIVAKDLVELYAVRQQSEGFVYGPDTVWQKEFEEMFP
                     FEETDDQLQAIEDTKHDMESKKIMDRLICGDVGYGKTEIAIRAAFKAVQEGKQVVCLV
                     PTTILAQQHYNTFVQRLKEFPVRIDLLCRFRSAAEQKKTIEDTKKGFVDILVGTHRVL
                     SKDVVFKDLGLLIIDEEQRFGVTHKEKIKKLKENIDVLTLTATPIPRTLHMSLIGIRD
                     MSVLEEAPNERMPIQTYVMEYNDEMVREAITRELARDGQVYYVYNRVNDIADVAGRIQ
                     SLVPDANVAFAHGQMKERELEDIMYDFINGDIDVLVSTTIIETGLDIPNANTMIIQDA
                     DRFGLSQLYQLRGRVGRSNRMAYAFLLYQRDKLLKEVAEKRLSAIREFTDLGSGIKIA
                     MRDLEIRGAGNLLGEAQSGHMEAVGYDLYCKMLNEAVRQLKGGPEAETFTTLIDLNVD
                     AYIPEYYIKNEYQKLDIYKRIAAIESEEELEDMTEELIDRFGDIPKKVQQLLVIASLK
                     SLAHSVYVTAIEQKGEEIRFTMYEKAKIDPSGIPKFLDSYKNDLIFRAEEPPYFLYCR
                     KGRNKKSNTENVLDTVRRILEDLKKLT"
     gene            complement(3453..4034)
                     /gene="pth"
                     /locus_tag="ACZPD4_04480"
     CDS             complement(3453..4034)
                     /gene="pth"
                     /locus_tag="ACZPD4_04480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1378361.1"
                     /translation="MFIIAGLGNPTKQYEGTRHNVGFEVIDRISEKYNIDVDAKKHRA
                     LIGKGIIQGQKVILAKPQTYMNLSGESVRSLLDYYKVDEEHELIVIYDDISLDVGQLR
                     IRAKGSAGGHNGMKNIIAHLGSQVFPRIKVGVGEKPAKYDLADYVLGHFSKGEAKLMD
                     EGYDHAVHAVELIVSGQINEAMNEYNRKKKEEK"
     gene            4186..4620
                     /locus_tag="ACZPD4_04485"
     CDS             4186..4620
                     /locus_tag="ACZPD4_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1378362.1"
                     /translation="MSSRTKIVVLHMKEIIYTAIFAALALLVIVLLIFMFGTGKKDSA
                     VKNEKQYTPGTYTSTLTLNNTNLEVEVSVDAYKINSIRFSNLDETVTTMFPLIQPAIE
                     EIAEQIYETQSPDSITLSEDTPYTSQLILDAVSEAVEKAAVK"
     gene            complement(4702..4965)
                     /locus_tag="ACZPD4_04490"
     CDS             complement(4702..4965)
                     /locus_tag="ACZPD4_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HIR1378363.1"
                     /translation="MNEYSEECLNTFLKEQKKLFDEPVAENMEEAEAFLADCMAVVVD
                     SIDEVREYFEEEGVDVDGMSAEDLEEASEVFALPDGTYLIVEG"
     gene            complement(4981..5634)
                     /locus_tag="ACZPD4_04495"
     CDS             complement(4981..5634)
                     /locus_tag="ACZPD4_04495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1378364.1"
                     /translation="MYKACIFDLDGTLTDTLESLTFSVNETLKEVALPAITEEQCRTF
                     VGNGAKVLIEQSLKAAGDTKLLLFEKAFEAYGRIFDRNCTYHVKPYPGIREMLLKLKD
                     QNMKLGVLSNKPDRQAVHVVEEIFGKETFDLVWGQREGVPRKPDPSALFAMAESLGVS
                     SEEVLYIGDSEVDAATGNAADMDTVLVEWGFRSREELEKADAYRIVAAADEILDIIR"
     gene            complement(5750..6532)
                     /gene="sigG"
                     /locus_tag="ACZPD4_04500"
     CDS             complement(5750..6532)
                     /gene="sigG"
                     /locus_tag="ACZPD4_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HIR1378365.1"
                     /translation="MTVNKVELCGVNTAKLPLLKEEEKEELFERIKAGDEEAREKYIK
                     GNLRLVLSVIKRFENSSENADDLFQIGCVGLMKAVDHFDPGRMVKFSTYAVPMIIGEI
                     RRYLRDNSPIRVSRSLRDTAYKAIYAKEGYIKRHMKEPTVQEIAEEIGIAKEEIVYAL
                     DAIQMPMSLHEPVYSDGQDALFVMDQVSDKKNKEENWIEELSLEAAMERLNERERYII
                     TLRFFEGKTQMEVAEQINISQAQVSRLEKNALKIMRKYLLGK"
     gene            complement(6672..8276)
                     /locus_tag="ACZPD4_04505"
     CDS             complement(6672..8276)
                     /locus_tag="ACZPD4_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR1378366.1"
                     /translation="MKLTFVGAAHEVTGSCHFLQAAGKNILIDCGMEQGPDLYENPGL
                     PIPENEIDYVFLTHAHIDHSGMLPKLAKNGFKGQIFTTFATADLCNIMLRDSAHIQEF
                     EAEWRNRKGRRAGKPEFEPVYVMQDALDAIELLVPCGYGERITICDGIQIRFTDVGHL
                     LGSASIEVWATEGDVTKKIVFSGDIGNVDQPIIKDPSYTDSADYIVVESTYGNRIHTA
                     EKPDYLGEFTRIIKETFDRGGNVVIPSFAVGRTQEMLYFIREIKEKHLLPEYEDFDVY
                     LDSPLAIEATKVFTMNMRDCFDKEAMELVNAGINPLVFPGLHISTTSDDSKMINFIEK
                     PKVIISASGMCDAGRIRHHLKHNLWRPECTILFVGYQANGTLGRSLIEGEKNVKLFGE
                     PIEVHAHIESLHGVSGHADMNGLLSWIGAFVSPIERVFVVHGEDTVTEEFAHTVEEKF
                     GYSAWAPYPCCEADLLKNEIVNEGVRVPVKAKKAARRKSDSAFERLVAAGRRLLDIIY
                     KNEGLSNKDKAKFETQINNLSDKWED"
BASE COUNT         2126 a         2226 c         1272 g         2696 t
ORIGIN      
        1 ttaaaatatc gtctgtaccg ttctttatca ccacggcaca acatgttttt agtataccgt
       61 gtttttattt ctatctcaag tcctaattta aaatcttaaa tttaagtcag cttttttagg
      121 tcttccaaaa tccgtctcac tgtatccagt acattttctg tattgctttt tttgtttctg
      181 ccttttctgc aatacagaaa atacggcggt tcttctgccc tgaatatcag atcgttttta
      241 taactatcca aaaatttagg aataccggat ggatcgatct ttgctttttc atacattgta
      301 aatcgaattt cttcaccttt ttgctcgata gcagtaacat acaccgaatg agccagactt
      361 tttaaagatg ctatcacaag aagctgctgc acttttttcg gaatatcgcc aaagcggtcg
      421 atcagttctt ccgtcatatc ttctaactct tcctcacttt caatcgccgc gatccgttta
      481 tatatatcca gcttttgata ttcattttta atataatatt ccggaatata tgcatctaca
      541 tttagatcaa tcaatgttgt aaacgtttct gcttccggcc cgccttttaa ttgtctgacc
      601 gcttcattta gcattttgca ataaagatca taccctaccg cttccatatg accgctctgc
      661 gcttctccta aaagattacc tgccccgcga atttcaaggt ctcgcatggc gattttgatt
      721 ccggatccga gatctgtaaa ctcacggatc gccgagagcc tcttctctgc cacttctttt
      781 aataatttgt ctctctgata cagaagaaat gcatatgcca tacgattaga acgaccgaca
      841 cgtccccgaa gctgataaag ctgagataat ccgaatcggt ccgcatcctg tatgatcatc
      901 gtattcgcat tcggaatatc caatccggtc tcaataatcg tcgttgaaac aagaacatcg
      961 atatcaccgt tgataaaatc atacatgata tcttccagct ccctctcttt catctgtcca
     1021 tgcgcaaatg caacattcgc atccggaaca agactttgta tacgtcccgc aacatcggca
     1081 atatcattga cgcgattata aacataatat acctgtccgt ctcttgccaa ctcccgcgtg
     1141 attgcctccc gaaccatttc gtcattatac tccattacat aagtctgaat gggcatcctt
     1201 tcattcggag cttcttctaa tacgctcata tcccgaatac cgatcagact catatgaagc
     1261 gtccggggaa tcggtgttgc cgtcaatgtc agaacatcaa tattttcctt taattttttt
     1321 attttttctt tatgggtcac gccaaaccgt tgttcttcgt ctattatcag caatccaaga
     1381 tctttaaaca ctacatcctt tgacaaaact ctgtgcgttc cgacaagaat atccacaaac
     1441 cctttcttcg tgtcctctat tgtctttttc tgttctgccg ccgaccgaaa ccgacagagc
     1501 agatcgattc ttacaggaaa ctctttcagt cgctgtacaa atgtattata gtgctgctga
     1561 gctaaaatcg tcgtcggcac gagacataca acctgttttc cttcctgaac cgctttaaat
     1621 gccgcccgta tagcaatttc tgtctttccg tatcctacgt ctccgcagat aagacgatcc
     1681 atgatcttct tactttccat atcatgcttt gtatcttcta ttgcctgaag ctgatcatcc
     1741 gtttcctcga acggaaacat ttcctcaaac tctttctgcc agacagtatc cggtccatat
     1801 acaaatcctt ccgactgttg tctgaccgcg tacaattcta caagatcttt cgctacaatc
     1861 tgaacagctt tctttacttg gctttttgtt tttccccact ggatcgttcc gagtttattc
     1921 aaccgaggtt tctttgcatc cgctcctgca tatttctgga tcagatccat ctgtgccaca
     1981 ggtatataca agactccgcc ttcggcatag acgatcttca tataatcttt tacgatcttc
     2041 tctacttcaa ttttttcaat tcctttatat acgccgattc catgattttc gtgaacaacg
     2101 taatctccgg gtttcagctc ggcaaaatcc tgaatccgct gaccttcata ctttttgcgt
     2161 tttttctttt tcttttttct tccgaaaata tccgactccg aaataacgac aaatttaagc
     2221 atcggatact catacccttc tgacgcatag ccgtacgctg ccatgatctc gcccggtttt
     2281 acttccctct gcatatcttc actgtaaaaa ctgctcaaat catagtctct taaatcttcc
     2341 gccagacgtt ttgctctcgt tctcgaaccc gataaaagga caactctgta cccgtttcgt
     2401 tttaaccgct ttaaatcacg tgtcaaaagc tcaaaactgt tattataagg atttacccct
     2461 tttgcctgca ccgtataaac cgatcttact ttaaataaac cgcattttga ttcgagcgtt
     2521 gtcaaaccga tcccgctgta acgattcatc tcactgatga tatcttttgt ctgaaagact
     2581 ctcaactctt cttcgccttc ttctttcata cctgcatcag cccggttttt caaactttcc
     2641 gtatattccg cctctactgt ttccgccgtc tcctgcagtc tgtgcggttc atcataaaat
     2701 aatagtgttt cattttccgg aaaatatttc aaaaaggata ccaaaccatt gtttccaaaa
     2761 caattttccg aagccggata aattgtgatc tccgaaatat tttctatcga cctctggctc
     2821 ttagcatcaa acatacggat agaatcgatc tcatcacccc aaagctcaat ccgcaccggc
     2881 atctcttccg caagaggaaa aacatcgata attccccctc tgaccgcaaa ctgacccggc
     2941 gcttctattt ggctttctct ctcatatcca agagccgtca gatcttgttc aagtttcgta
     3001 agatcaaccg tatcttctcc tgtaatatga attctgtttt tttgaatttc atccggggaa
     3061 atgatcccgt ccaaaaaagc atccatcgtc gtaatcacag tgaccggctc ttcttttttc
     3121 ttttctgcaa tcgctcttaa cacttccatt cttcgatttg taagtgtctt ccctttaata
     3181 tccgcataat aaaaaagcaa atctcttgca ggatacaaat acgtctcttc tccaaacagt
     3241 aaatactctt cgtatgcctt ctttgccttt tcctcatttg aaaaaacaac aagtttatac
     3301 ccgcatcccg atccgattcc actaagcaaa tgtatcttct gagtatttac acaacccgag
     3361 atctgtatca ttccgttttc ttttgttctg tttttccgga tttcttcata atccgccagt
     3421 tccgcaaggg gagcaagcaa cgctttcata atttattttt cctctttctt tttccggtta
     3481 tattcattca tcgcctcatt gatctgacct gaaacgatca attccacggc atgaaccgca
     3541 tggtcatacc cttcatccat caacttcgct tccccctttg aaaaatgtcc gagcacataa
     3601 tccgccaaat cgtatttcgc cggcttctct ccgactccta ctttaatacg gggaaatact
     3661 tggcttccaa gatgagcaat aatatttttc atcccgttat gtcctcctgc gcttcctttc
     3721 gctctgattc taagttgtcc tacgtctaag ctgatatcgt cataaataac gatcagttca
     3781 tgttcttcat ccaccttata ataatcaagc aaactgcgta cgctttcgcc acttaaattc
     3841 atataagtct ggggcttagc caaaataact ttctgcccct gaatgattcc ctttccgatc
     3901 agcgctctgt gtttttttgc atccacatct atattatatt tctctgaaat cctgtctatt
     3961 acttcaaatc cgacattatg tctcgttcct tcatattgtt ttgtcggatt tccaagtccc
     4021 gcgataataa acatatcttc tacctcctat acagcacaaa tattttacaa tatctctctt
     4081 cctttgtcac gcagataaca tgcatatttt tccttttcca aacatacact tataaaaaaa
     4141 gcggatcgcc gtaaaacggc agccggataa tcgggaggta actctatgag ttccagaaca
     4201 aaaatcgttg ttcttcacat gaaagaaatt atttataccg ccatttttgc ggcacttgcg
     4261 ctccttgtca tcgtcctgct tatttttatg ttcggaacag gcaaaaaaga cagcgccgta
     4321 aaaaacgaaa aacagtacac gcccggaaca tatacttcca ctctcacact aaataatacg
     4381 aacctggaag tagaagtttc cgtagacgct tacaagatca attcaatccg tttttccaat
     4441 cttgatgaaa cagtaacgac tatgttccca ctcatccagc ctgccatcga agaaattgcc
     4501 gaacaaatct atgaaacaca gtcacctgat tcaatcactc tgtccgaaga tacgccatat
     4561 acgtcgcaac ttatattgga cgccgtttcc gaagccgtcg aaaaagccgc agtaaaataa
     4621 ctgcggcctt ttctctttgc tgccttatat tcatacaaca atacttgaca aatattattc
     4681 atacatttgc tttcaccggt attatccttc aacgatcaaa tacgttccgt ccggcaaagc
     4741 gaacacttcc gaagcttcct caagatcttc tgcggacatt ccgtccacat ctactccctc
     4801 ttcttcaaag tattctctca cctcgtcaat agaatcgact acgacagcca tacagtctgc
     4861 aagaaacgct tctgcttctt ccatgttttc tgccacaggc tcatcaaata attttttctg
     4921 ttctttcaaa aatgtgttca gacactcttc actgtactca ttcatctgtc tttttcccct
     4981 ttacctgata atgtcaagaa tttcatctgc cgctgccaca attctgtatg catctgcctt
     5041 ttccagttct tctcgtgatc gaaatcccca ttctacaaga accgtatcca tatccgccgc
     5101 atttcccgtc gcagcgtcga cctctgaatc tccgatatac aatacctctt ccgaagacac
     5161 ccctaatgac tctgccatcg caaacaatgc cgaaggatcc ggttttctcg gtacaccttc
     5221 tctctgcccc cacacaaggt caaacgtctc tttcccgaat atttcttcca caacatgaac
     5281 tgcctgacga tccggtttgt tcgacaacac tcctaatttc atgttttgat ctttcagctt
     5341 taagagcatc tctcttatcc ccggataagg ttttacatga taggtacagt tcctgtcaaa
     5401 aatacgtcca tatgcctcaa aggctttctc gaacaaaaga agctttgtat ctcctgccgc
     5461 tttcaatgat tgttcaatca gtacctttgc tccgtttccg acaaatgttc tgcactgttc
     5521 ctctgtgatc gccggaagcg ctacttcctt taatgtctca ttcaccgaaa atgtcaaaga
     5581 ctccaacgta tcggtcaacg ttccgtctaa gtcaaatata catgctttgt acaccgtttt
     5641 tcatcctctc ttgtctttgg tcagatttat attaccatca gtcactttat ctgacaagat
     5701 acttttaaac aatctgttct ttctcctgac gtattgtttt ttcgcactct tattttccta
     5761 aaagatactt gcgcattatt tttaaagcat tcttctccag acggcttacc tgagcctgcg
     5821 aaatattgat ctgctctgca acttccatct gcgtctttcc ttcaaaaaaa cgaagtgtga
     5881 taatatatcg ctctctttcg ttcagtcgtt ccatcgcggc ctctaaagaa agttcctcga
     5941 tccagttttc ttctttattc tttttatcac tcacctgatc catcacaaac agagcatctt
     6001 gtccgtcgct gtacacaggc tcatgcagac tcatcggcat ctgaatcgca tcaagcgcat
     6061 agacgatctc ttccttggca atcccaatct cttctgcgat ttcctgaacg gtcggctctt
     6121 tcatatgccg ctttatataa ccttcttttg cataaattgc cttataagct gtatcgcgca
     6181 atgatctgct cacacggatc ggactgttat cccgcaaata tcgtctgatc tcccctatga
     6241 tcataggtac tgcatatgtg gaaaatttca ccatgcgccc cggatcaaaa tgatctactg
     6301 ctttcatcag tccgacacat cctatctgga acagatcatc cgcattttca ctgctgtttt
     6361 caaaacgttt gatcacactt agaacaagcc gcagattccc tttaatatac ttttcccggg
     6421 cttcttcatc ccctgctttt atccgctcaa aaagttcttc tttttcctct tctttaagaa
     6481 gcggaagctt cgctgtattt accccgcaaa gttctacctt atttactgtc atcacaacaa
     6541 tcctcccaag tagtttctac ttagaagaat tctcattcaa taagaaagat attccgattc
     6601 ccgcaaattt ttataaactt ttagtccttg acaaaatcat ctttttttgt atttcccgtc
     6661 acgcagacca tttagtcttc ccatttatcg gaaagattat tgatctgtgt ttcaaatttt
     6721 gccttatctt tattagaaag tccttcattt ttgtaaataa tatcaagcag acgtctgcct
     6781 gctgccacaa gccgttcaaa agcagagtct gatttccgcc gtgccgcttt ctttgctttg
     6841 accggaacgc gcactccttc gttcacgatc tcattcttca aaaggtccgc ttcacagcaa
     6901 gggtaaggcg cccatgcaga atacccgaac ttttcctcta ccgtatgtgc aaactcttct
     6961 gtcaccgtat cttctccatg tacgacaaac actctctcaa tcggactgac aaacgcccca
     7021 atccatgaaa gcagcccgtt catatccgca tgaccgctta ccccgtgtaa gctttcgata
     7081 tgcgcatgaa cttcaatcgg ctctccaaac agttttacat tcttttctcc ttcaatcaga
     7141 cttcgtccga gagttccgtt tgcctgatat cccacaaaca ggatcgtaca ttccggacgc
     7201 caaagattat gcttcaaatg atgacggata cgacctgcat cgcacattcc cgatgcggat
     7261 ataatcactt tcggtttctc gataaaattg atcatcttgg aatcatcgct cgttgtcgat
     7321 atgtgaagcc cgggaaatac aagaggattt attccggcat tgacaagctc cattgcctct
     7381 ttatcaaaac aatcccgcat attcattgta aatactttcg tcgcttcgat cgccaacgga
     7441 ctatccaggt acacgtcaaa atcttcgtat tccggaagca gatgcttttc tttgatctca
     7501 cggataaaat aaagcatctc ctgtgtcctt ccgactgcaa aggaaggaat gacaacatta
     7561 cctcctctgt caaatgtctc cttgataatg cgggtaaact ctcccagata atccggtttc
     7621 tcggcagtat gtatccggtt tccgtaagta gactccacta cgatataatc tgccgaatct
     7681 gtgtagcttg gatcttttat gatcggctga tctacatttc cgatatcccc cgaaaagacg
     7741 atcttcttcg tcacatcacc ttctgttgcc cagacttcga tactggccga tccaagcaga
     7801 tgtccgacat cggtaaaacg aatctgtatc ccatcgcaga tcgtgatcct ttccccatac
     7861 ccgcacggca cgagcagttc aatcgcatcc aacgcatcct gcatcacata cacgggctca
     7921 aattccggtt ttcctgccct tctgcctttc ctgtttcgcc attctgcctc aaactcctga
     7981 atatgagcgc tgtcacgaag catgatatta catagatcgg cagttgcaaa tgttgtgaaa
     8041 atctgacctt taaatccgtt ttttgccaat ttcggcagca ttcccgaatg atcgatatgt
     8101 gcatgagtca aaaatacata gtcaatctca ttttccggaa tcggaagtcc cggattttca
     8161 tacagatccg gtccttgttc cattccgcag tctatcaaga tattttttcc tgccgcttga
     8221 agaaaatgac agcttcccgt aacttcatga gctgccccga caaatgtaag cttcataagt
     8281 ccaccatccc ttttcacttt tcttctattg taacatcatt
//
LOCUS       DBMDXF010000078         8313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2295_length_8313_cov_2.196420, whole genome shotgun sequence.
ACCESSION   DBMDXF010000078 DBMDXF010000000
VERSION     DBMDXF010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_8313_cov_2.196420"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="ACZPD4_04510"
     CDS             complement(<1..511)
                     /locus_tag="ACZPD4_04510"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR1378367.1"
                     /translation="MANIDLSQYGITGTTEIVYNPSYELLFKEETDSSLEGFEKGQES
                     ELGAVNVMTGIYTGRSPKDKFIVMDENSKDTVWWTSDEYKNDNHPASQEAWDTCKAIA
                     LKELSNKRLFVVDAFCGANADTRMAIRFIVEVAWQAHFVKNMFIQPTEEELENFKPDF
                     VVYNASKAKV"
     gene            complement(860..2347)
                     /gene="thrC"
                     /locus_tag="ACZPD4_04515"
     CDS             complement(860..2347)
                     /gene="thrC"
                     /locus_tag="ACZPD4_04515"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1378368.1"
                     /translation="MNLLYKSTRNSEKTVTASEAILKGLADDGGLFVPESIPKLDVTL
                     DELKGMSYQETAYAVMKQFFTDFTEEELKHCINSAYDSKFDTEVIAPLVKVGDTYHLE
                     LFHGATIAFKDMALSILPHLLITSAKKNNVKNEIVILTATSGDTGKAALAGFADVPGT
                     RIIVFYPKGGVSRIQELQMVTQKGENTSVVAIHGNFDHAQSGVKAIFEDKKLAEELAD
                     AGYQFSSANSINIGRLVPQVVYYVYAYAKLLENEEIKAGEKINVTVPTGNFGNILAAY
                     YAKQMGVPIAKLICASNENKVLFDFFKTGVYDKNREFILTSSPSMDILISSNLERLIY
                     TIAGQDAGKNAELMRQLKEEGVYEITEEMRGQLGDFVGGFATEEETKETIKNTYEKTG
                     YVMDMHTAVAAHVCAQYRKESGDEKKCLVASTASPYKFIHSVMTAIDDKYAKKDEFEL
                     IDELSALSATEIPNAIKEIRNAQIRHNRECDSDKMKEIVKEILNV"
     gene            complement(2406..3698)
                     /locus_tag="ACZPD4_04520"
     CDS             complement(2406..3698)
                     /locus_tag="ACZPD4_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1378369.1"
                     /translation="MGNEKQKGKAFALLPIGVFLVIFLGAGILFKDFYAMPAVVAFLI
                     ALFVAFLQNRKVSFQKKMEVIAKGLGEENIITMCLIFLCAGAFSGAVTAAGGVDSTVN
                     LGLSLIPPHFAVAGLFIIGCFISVSMGTSVGTITALAPIAVGISKQTGFSMAICVAAV
                     VCGAMFGDNLSMISDTTIAAVKTQGCEMKDKFKMNFLIVLPAAIITVVFFWFVTRNVA
                     YDITENLDYNFWQVVPYLVVLIGALIGINVFLVLIGGTVISLIAGVATGNIVLADMFT
                     VVGDGVVSMYDITVISILVACIVSLVKMNGGIQFILDQIKSRIHGAKGAQGGIALLSF
                     LVDLCTANNTVAIVMTGPIAKEISDEFDVDPRRSASLLDMFTSVGQGLIPYGAQLLTA
                     AALTGLTPFDFMPYLIYPMLMAVCGIVAICIRRERKSA"
     gene            complement(3810..4301)
                     /gene="leuD"
                     /locus_tag="ACZPD4_04525"
     CDS             complement(3810..4301)
                     /gene="leuD"
                     /locus_tag="ACZPD4_04525"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1378370.1"
                     /translation="MRAHGHVFKYGDNVDTDVIIPARYLNSFDAQELASHAMADIDPD
                     FVKKVQSGDIIVANKNFGCGSSREHAPLCLKTAGVSCIIAETFARIFYRNAINIGLPI
                     IECPEAAQGIEAGDEVEVDFDSGMIYNRTKGTEFKGQPFPEFMQKLIAAGGLVNYTNS
                     KKK"
     gene            complement(4298..5632)
                     /gene="leuC"
                     /locus_tag="ACZPD4_04530"
     CDS             complement(4298..5632)
                     /gene="leuC"
                     /locus_tag="ACZPD4_04530"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1378371.1"
                     /translation="MGMTMTQKILAAHAGLDTVEAGQLIEAKLDIVMANDITGPMALP
                     IIRQMSDKVFDKDKVVFVPDHFTPNKDIKSAENSKAIREYAKEQGLKWYFEQGKSGVE
                     HAILPEAGVVTAGECIIGADSHTCTYGALGAFSTGMGTTDVATGMATGEIWFKVPSAI
                     KFVLTGKPGKYVSGKDIIIHIIGKIGVDGALYKSMEFTGDGIGNLSMDDRFTMANMAI
                     EAGAKNGIFPVDEKTVEYLDAHCEKPYTVYEADEDAVYDDVVEIDLSKVRPTVAFPHL
                     PGNAKTIDEIEAEEQVKIDQVVIGSCTNGRMEDMRKAAAILKGHTVHPDVRVMVVPAT
                     QKIYKQCIKEGLLDIFVDAGCAVNTPSCGPCMGGHMGVMAAGEKCVSTTNRNFVGRMG
                     HVDSLIYLASPEVAAASAIKGYIANPEKISAADEEADSRNIQCAEQKEGGRS"
     gene            complement(5683..6768)
                     /gene="asd"
                     /locus_tag="ACZPD4_04535"
     CDS             complement(5683..6768)
                     /gene="asd"
                     /locus_tag="ACZPD4_04535"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1378372.1"
                     /translation="MEQKLKVGILGATGMVGQRFISLLEDHPWFEVVTVAASPRSAGK
                     TYEEAVGDRWKMDTPMPEAVKKLVVLNVNDVEHVASTVDFVFSAVDMSKEEIKVIEEA
                     YAKTETPVVSNNSAHRWTPDVPMVVPEINAEHFEVIKDQKKRLGTTRGFIAVKPNCSI
                     QSYAPCLAAWKEFGPKELVVTTYQAISGAGKTFKDWPEMLGNIIPFIGGEEEKSEQEP
                     LRVLGKVENGEIVKAELPKITCQCVRVPVLNGHTAAVFINFEKKPTKEQLIEKLVNFK
                     GFPQEAELPSAPKQFIQYLEEENRPQVTADVDYERGMGVSIGRLREDSMYDFKFIGLS
                     HNTVRGAAGGAVLCAEALTAKGYIAKK"
     gene            complement(6901..8289)
                     /gene="argH"
                     /locus_tag="ACZPD4_04540"
     CDS             complement(6901..8289)
                     /gene="argH"
                     /locus_tag="ACZPD4_04540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1378373.1"
                     /translation="MAQLWGGRFTKETDQLVYNFNASISFDKRFYKQDIRGSIAHVTM
                     LEKQGILTKEEKDQIIEGLESICRDVENKTLEITEEYEDIHSFVEANLIDRIGDAGKK
                     LHTGRSRNDQVALDMKLYTRDEITHLDSLLRELMEVLLKLMEENTETYMPGFTHLQKA
                     QPITLAHHMGAYFEMFKRDHQRMRDIYKRMNLCPLGSGALAGTTYPLDREYTALLLGF
                     DGPTLNSMDSVSDRDYLIELLSAMSTIMMHLSRFSEEVIIWNTNEYKFVEIDDAYSTG
                     SSIMPQKKNPDIAELVRGKTGRVYGALTSLLTTMKGIPLAYNKDMQEDKELVFDAIDT
                     TKGCLALFTGMLKTMRFNNARMEESAKHGFTNATDAADYLVNHNVPFRDAHGIVGRLV
                     LYCIEKNIALDDMSLEEFKAISPVFEEDIYEAISMKTCVDMRNTIGAPGKNAMEAVIR
                     AERQYLKEQPAL"
BASE COUNT         2159 a         2193 c         1369 g         2592 t
ORIGIN      
        1 ccacttttgc ttttgatgca ttgtatacaa caaaatccgg cttaaagttc tcaagctctt
       61 cctctgtcgg ctgaataaac atattcttta caaaatgagc ctgccaagca acctctacga
      121 taaaacggat tgccatacgt gtatctgcat ttgctccgca aaatgcgtca acaacaaaca
      181 gtctcttatt ggaaagttct ttcagcgcga tcgccttaca tgtatcccat gcttcctggc
      241 ttgccggatg attgtcgttt ttgtattcat ctgatgtcca ccaaaccgta tctttggagt
      301 tttcatccat tacgataaat ttatctttcg gagaacgtcc tgtataaata cctgtcatta
      361 cattaacagc tcccagttcg ctttcctgtc ctttttcaaa gccttcaaga cttgaatctg
      421 tctcttcttt aaaaagcagc tcataagaag ggttgtaaac gatttccgta gttcctgtaa
      481 taccatactg actaagatca atgtttgcca taatatgtct tacctctctc tttctaaaat
      541 gtatatttca catctacgcc gcttaatgcc agcgttatgc tttcattata caaaatcatt
      601 ttccaaaaat caacaataat attcatctat ctctctcgat tatatgaaag ttttataaac
      661 tgcaaattca tatgctctat tttatttcta aaaaaaacaa aattacattt caatcaccgg
      721 aagtgtcaaa aggcacattt gtggatttta agcgattgtc aaggtttccg aaaaatctgg
      781 gttttgactc gtcgccattt aaaaaatgca ggattttttt atcaatcctg cattttctac
      841 acgacaagcc tctcataaat tatacattca aaatttcttt cacgatttct ttcattttgt
      901 ccgaatcgca ttctctgtta tgtctgatct gagcatttcg gatctctttg atcgcattcg
      961 gaatttccgt tgccgacagg gcgctcagct catcgatcaa ttcaaattca tctttctttg
     1021 catatttatc gtcaatggca gtcataacgc tatggataaa tttatacgga ctcgccgtcg
     1081 aagctacgag acacttcttt tcatccccac tttccttgcg gtattgtgcg catacatgtg
     1141 ccgctaccgc cgtatgcata tccatcacat accctgtttt ctcatatgta tttttaatcg
     1201 tctctttcgt ctcttcttcc gtcgcaaatc ctccgacaaa atcacctaac tgtccgcgca
     1261 tctcttctgt tatttcataa acgccttctt ctttaagctg tctcatcaac tcggcatttt
     1321 tccctgcatc ctgccctgcg attgtataga tcaaacgctc cagattgctg gaaatcaaaa
     1381 tgtccataga cggggaagat gtcaggataa attctctgtt tttatcataa acccctgttt
     1441 taaagaaatc aaaaagcact ttattttcgt tggatgcaca gatcagcttt gcaatcggca
     1501 ctcccatctg tttcgcataa taagcagcca gaatgtttcc gaagtttcct gtcggaaccg
     1561 tcacattgat cttttctccc gctttgattt cttcattctc aagcagtttt gcataagcgt
     1621 aaacataata tactacctgc ggaacaagac gtccgatatt gatagagttc gcagatgaaa
     1681 actggtatcc ggcatctgcc aactcttccg ccagcttttt atcttcaaaa atcgctttca
     1741 caccgctttg tgcatgatcg aaatttccgt ggatcgccac tactgatgtg ttctctcctt
     1801 tttgagtcac catctgaagc tcctgaatac ggctgacgcc cccttttgga taaaaaacga
     1861 tgatcctcgt tcccggaacg tccgcaaaac ctgcaagagc cgcctttccc gtatctcctg
     1921 atgttgccgt taaaattaca atttcatttt tcacattatt tttcttagcc gacgtaataa
     1981 gaagatgcgg caaaatcgac aaagccatat ccttaaatgc gatcgttgct ccgtggaaca
     2041 actccaaatg atatgtgtca cccactttta caagcggagc gatcacttct gtatcaaact
     2101 tggaatcata cgcactgttg atgcagtgtt ttaactcttc ctccgtaaaa tccgtaaaaa
     2161 actgtttcat cacagcataa gctgtttcct gataagacat ccctttcaat tcatcaagcg
     2221 taacgtcaag ctttggaata gattccggaa caaacaatcc cccgtcatct gccagacctt
     2281 tcaagatcgc ctcggatgcc gtcactgttt tttctgagtt tcttgtactc ttatacaata
     2341 aattcattat tttacctctt tttctttctg caacacaatt ttcaactaaa tatattcctg
     2401 aatactcagg cactttttcg ttcccgcctt atgcagatcg ccacaatacc gcatactgcc
     2461 atcagcatcg gatagatcaa atacggcata aaatcaaacg gagtcagacc tgtaagagcc
     2521 gcagccgtaa gaagctgagc gccgtaaggg atcagcccct gtccaacgga tgtaaacata
     2581 tccagcagag atgccgatct tcttggatca acgtcaaact catcactgat ttccttcgcg
     2641 atcgggcctg tcataacaat cgctaccgta ttgtttgccg tacaaagatc aactaaaaac
     2701 gaaagcagcg caattcctcc ctgtgctcct tttgctccgt gaatcctgct tttgatctga
     2761 tccagaataa actggattcc gccgttcatc ttcacaagcg atacaataca tgctacaaga
     2821 atagatatta cagtaatatc atacatcgat acaacgccat ctcctacaac agtaaacata
     2881 tccgctaaaa caatgtttcc cgttgccact cccgctataa gtgaaataac agttccgccg
     2941 atcagcacga gaaatacatt gattccgatc aacgcaccta taagcacgac aagatacggt
     3001 acaacctgcc agaaattata gtctaaattc tctgtaatat cgtacgctac attgcgcgtc
     3061 acaaaccaaa agaacacgac tgtaatgatc gccgccggaa gtacgatcag aaaattcatc
     3121 ttaaacttat ctttcatctc gcatccctgc gtttttactg ccgcaatcgt cgtatcagag
     3181 atcatcgaca ggttatcgcc gaacattgcc ccgcacacga cggcagctac acagattgcc
     3241 atagaaaacc ctgtctgttt actgattccg accgcaatcg gagcaagcgc cgtgattgtt
     3301 cctacggaag ttcccatcga cactgaaata aaacatccta taataaacag acctgccact
     3361 gcaaaatgcg gcggaataag agagagtccc aaattcaccg tactgtccac tccgcctgct
     3421 gcggttaccg ctcccgaaaa agctcccgcg caaaggaaaa taagacacat agtaataata
     3481 ttttcctctc cgagtccttt tgcgatcact tccatctttt tctgaaaact tactttacgg
     3541 ttctgcaaaa acgcaacaaa taaagcaatc agaaatgcaa ctactgccgg cattgcgtaa
     3601 aaatccttaa acaagattcc cgcgcctaaa aagattacca aaaacacgcc tataggcaga
     3661 agcgcaaaag cctttccctt ttgtttttca tttcccatta aattctccct ttcgttttag
     3721 atataataac cgttgcaaaa aggcggcagt actgcctgcc gcttttcttt ttgcccgaat
     3781 ttcggctaaa agagccaaaa ctttatatat tatttctttt tactgtttgt atagttgacc
     3841 aatccgcctg cggcaatcaa tttctgcata aattccggga agggctgacc tttaaattca
     3901 gttccttttg tacgattgta aatcatacca ctgtcaaaat caacctcaac ttcgtcacct
     3961 gcttcaattc cctgtgccgc ttccggacat tcaataatcg gaagaccgat attgattgca
     4021 tttctgtaaa agattcttgc gaacgtttcc gcgataatgc agcttactcc tgccgttttc
     4081 aagcagagcg gcgcatgttc tctggatgat ccgcatccga aatttttatt tgccacaata
     4141 atatctcccg actgtacttt ttttacaaaa tccggatcta tatctgccat agcgtggctg
     4201 gcaagttctt gtgcatcaaa ggaattcaga tatcttgccg gaatgataac gtctgtatct
     4261 acattgtctc cgtatttaaa aacatgtccg tgcgctctca tgatcttcct ccttctttct
     4321 gttctgcaca ttgaatattc cgcgaatctg cctcttcgtc tgcagcgctt attttttcgg
     4381 gatttgcaat ataccctttg atcgcacttg cagcggcaac ttccggcgat gccagataaa
     4441 tcagagaatc aacatgtccc atgcgtccga caaaattacg gttcgttgtg gaaacacact
     4501 tttctcctgc tgccataact cccatatgcc cgcccataca cggtccgcag ctcggagtat
     4561 ttacagcaca tcctgcatct acaaaaatat ccagcagtcc ttctttgata cattgcttat
     4621 aaatcttctg tgtagccgga acaaccatca cacgcacatc aggatgtacc gtatgacctt
     4681 tcagtattgc cgcagctttc ctcatatctt ccattcgtcc gttcgtacaa gaaccaatca
     4741 caacctgatc gatcttaacc tgttcctctg cctcgatctc atcaatcgtc tttgcgtttc
     4801 ccggcaaatg cggaaacgct accgtcggac gcacctttga cagatcaatc tctacaacgt
     4861 cgtcataaac cgcatcctcg tccgcctcgt aaactgtata cggtttctca caatgagcgt
     4921 caagatactc gaccgtcttt tcatccaccg gaaaaatccc gtttttcgca ccggcctcaa
     4981 tcgccatatt cgccatcgta aaacgatcat ccatagacag atttccaatt ccgtcacctg
     5041 taaattccat tgatttataa agcgcgccgt ccactccgat cttgccgata atgtgaatga
     5101 taatatcttt tccgcttaca tatttccccg gctttcccgt cagaacaaac ttgatcgccg
     5161 acggcacttt aaaccagatt tctcctgtcg ccattccggt tgccacatcg gtcgttccca
     5221 ttcctgttga aaacgcgccc aaagctccgt atgtacatgt atgtgaatcc gctccgatga
     5281 tacactcacc tgcggtgaca actcccgctt cgggaagaat cgcatgttcc acacccgatt
     5341 ttccctgttc aaaataccat ttcagcccct gctccttcgc atattcacga attgcttttg
     5401 aattttccgc tgatttgata tctttattcg gtgtaaaatg atccggtaca aacacaacct
     5461 tatctttatc aaatacttta tccgacatct gtctgatgat cggcaaagcc atcggtcctg
     5521 taatatcatt cgccatcacg atatcgagtt tcgcttcgat cagctgccct gcttccacag
     5581 tatccaatcc tgcgtgagca gccaaaatct tctgcgtcat cgtcattccc atttttgaga
     5641 ccccctttat cattcataaa tatggtcttt gccacatcat ttttatttct ttgcgatata
     5701 tccttttgcc gtaagcgctt ctgcacaaag cactgctccg ccagccgcac ctcttactgt
     5761 attatgtgac agtccgataa acttaaagtc atacatgctg tcctctctga gacgtccgat
     5821 agacactccc atgccgcgct cataatctac gtctgccgtt acctgcggac gattttcttc
     5881 ctcaagatac tggataaact gcttcggcgc gctcggcagt tctgcttcct gcgggaatcc
     5941 tttaaagttt accagttttt caattagctg ttcttttgtc ggtttctttt caaaattgat
     6001 aaataccgcc gcagtatgtc cgtttaatac aggaacacgc acacactgac atgtgatctt
     6061 cggaagctcg gcttttacga tctcgccatt ttccactttt ccgagaactc taagcggctc
     6121 ctgctcgctt ttctcttctt ctcctccgat aaacggaata atatttccca gcatttccgg
     6181 ccagtctttg aatgtttttc ctgctcctga aattgcctga tatgttgtaa caacaagctc
     6241 tttcggtccg aattctttcc atgccgccaa acacggagcg taactttgga tcgaacagtt
     6301 cggttttact gcaatgaatc ctctcgttgt accgagacgt ttcttctgat ctttgatcac
     6361 ttcaaaatgt tctgcattga tctccggtac taccatcgga acgtccggcg tccagcgatg
     6421 cgcgctgttg tttgatacga ccggagtctc tgtttttgca tatgcctctt cgatcacttt
     6481 gatctcttct ttgctcatat caaccgcgct gaaaacaaaa tctacagtcg aagctacatg
     6541 ctctacgtca tttacgttaa gaacaacaag ctttttcaca gcctccggca tcggcgtatc
     6601 cattttccag cgatcgccta ccgcttcctc atatgtcttt cctgcagaac gcgggcttgc
     6661 cgcaactgtt acaacttcaa accacggatg atcctcaagc agagagataa atctctgacc
     6721 taccattcct gtcgcaccta aaatacctac ttttaatttc tgttccatct tctttctcct
     6781 tttactgtta tttttcctgt tatctttctt actatagcgc catatccacg tcagcttccg
     6841 cttgcctcta taacctctat agtatcttct atctgtaaaa gcctgactct gcgaaattct
     6901 ttataaagca ggctgttctt tcaaatattg ccgttctgct ctgatcaccg cttccattgc
     6961 gtttttcccc ggcgcgccga ttgtgttacg catatccacg catgttttca tactgatcgc
     7021 ctcatatata tcttcttcaa agacaggaga aatcgccttg aattcttcca aagacatatc
     7081 gtccaaagca atatttttct caatgcaata caatacaagt cttccaacga taccatgggc
     7141 atccctgaac ggaacattgt gatttacgag ataatccgca gcatccgtcg catttgtgaa
     7201 accgtgcttt gcactttctt ccatgcgggc attgttaaaa cgcattgtct ttaacattcc
     7261 tgtaaataat gcaagacatc ctttggtcgt atcgatcgca tcaaatacaa gttctttatc
     7321 ttcctgcata tctttattat atgcaagcgg aatccctttc atcgtcgtca aaagtgaagt
     7381 gagagctccg taaacgcgtc ctgtttttcc tcgtacaagc tctgcgatat caggattttt
     7441 cttttgcggc ataatactgc ttcccgtact gtaagcgtcg tcaatctcaa caaatttgta
     7501 ttcatttgta ttccagatga tcacttcttc cgaaaatctg ctaaggtgca tcataatcgt
     7561 tgacatggca gacagaagtt caatcagata gtctctgtcc gacaccgaat ccatactgtt
     7621 taaagtcgga ccgtcaaatc cgagaagcaa agctgtatat tctctgtcca acggatatgt
     7681 cgttcccgcc aaagctccgc ttccgagagg acaaaggttc atccttttat aaatatcccg
     7741 catcctctga tggtcgcgct taaacatttc aaaataagct cccatatgat gtgccagagt
     7801 aatcggctgc gccttttgca gatgtgtaaa tcccggcata tatgtctctg tattttcttc
     7861 catcaatttc aaaagaactt ccataagttc tcttaacaaa ctgtcaaggt gtgtgatctc
     7921 atctcgcgta taaagcttca tatccaaagc gacctgatcg tttctgcttc gccctgtatg
     7981 taattttttc cccgcatctc cgatcctgtc gatcagattc gcttctacaa agctgtggat
     8041 atcttcatat tcttctgtga tctcaagcgt tttgttctct acgtctctgc atatactttc
     8101 aagaccctct atgatctgat ctttctcttc ttttgtcaga atcccctgtt tttcaagcat
     8161 cgtcacatga gcaatgctcc ctctgatatc ctgtttataa aaacgcttat caaaagaaat
     8221 cgaagcgtta aaattataaa ctaactgatc tgtttctttc gtaaaacgac caccccatag
     8281 ttgtgccatt tcatctcctc ttttctgttc tgc
//
LOCUS       DBMDXF010000079         8301 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2296_length_8301_cov_1.920488, whole genome shotgun sequence.
ACCESSION   DBMDXF010000079 DBMDXF010000000
VERSION     DBMDXF010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2296_length_8301_cov_1.920488"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            367..1629
                     /gene="uraA"
                     /locus_tag="ACZPD4_04545"
     CDS             367..1629
                     /gene="uraA"
                     /locus_tag="ACZPD4_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HIR1378374.1"
                     /translation="MENKKIIQVEEKVPFKLLVPLSIQHMFAMFGASVLVPFVFGLNP
                     AVVLFMNGIGTLLFILITKGKAPAYLGSSFAFLAPAGIVIEKWGYSYALGGFVAVGFL
                     GCVLALIIRKFGSKWIDVVLPPAAMGPVVALIGLELAGTAASNAGLTASSIDPKNVIV
                     FLVTLLTAVLGSVLFRKFFAVIPILIAIIAGYIAALLCGIVDFSKVASASFFALPNFS
                     TPKFKWEAIVIILPVILVIASEHIGHQIVTSKIVGRDLLKDPGLHRSLFADNFSTMIS
                     GFIGSVPTTTYGENIGVMAMTKVYSVYVIAGAAVLSIICSFIGKMTTLISTIPGPVIG
                     GISFLLYGMIGTSGIRILVDSQVDYARSRNMAMTSIIFVTGLSGIKVQFGSIQLTGMV
                     LACVVGMIMGLMFFLFDKLKLTNDRENC"
     gene            1674..2414
                     /gene="yaaA"
                     /locus_tag="ACZPD4_04550"
     CDS             1674..2414
                     /gene="yaaA"
                     /locus_tag="ACZPD4_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR1378375.1"
                     /translation="MLKIVISPAKKMNITDEFPCNATVPVFSDRADVLHGILKNKNIQ
                     ELKTLWKCSDKLAEQNYQRLHAFSPDQAVTPALLAYEGIQYQHIAPSVFTDAQWHYVN
                     VHLRILSGFYGILKPTDKVIPYRLEMQAKLEAAKKNDLYEYWSDTLYQSLLAEGMTEL
                     VNLASAEYSKVILPYKNIRCITCIFGEEVNGKIKVKGTQAKIARGEMVRWMADQKIES
                     VSDIREFKELGYRFSPSHSTEDTYTFSL"
     gene            complement(2571..4460)
                     /locus_tag="ACZPD4_04555"
     CDS             complement(2571..4460)
                     /locus_tag="ACZPD4_04555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIR1378376.1"
                     /translation="MEKMRFESVDGFQENIRRLADIFPSCVKEQKDNDGKTIRKVDIN
                     TLQLLLGINQKESETCEFSWVGKREAVKEVYKPIRKTLRPVVQDSVEWDSTGNLYIEG
                     DNLDVLKLLQESYLDSVKVIYIDPPYNTGNDFIYADDFRIRAREYVNAGGASQDDKNR
                     MYQNLDYSGRYHSDWCSMIYARLLAARNLLCDDGVIFISIDDNEQANLKKICDEVFGE
                     RNFVNCFIWNCSTAGGIRPKFASKTHEYILCYAKNKTCLDMFFAPLSGEAIKMYREKD
                     ERGLYRDKDFVFKNKSTNANQKYEIICPDGERVRPKDGYIYRFVRERFEQAKEEGLVT
                     FKRTKTGPLVDQNGDQAHWNIYIKKYLGDAMGAPSTLIPKEAVSIYNAGTQCVQELFD
                     GKRVFENVKPVNVIQYFINMAAKDGDIVMDFFSGSATTAHAVMQTEADKNIDLHYILV
                     QIPQECDKKSEAYKAGYKTICEIGKERIRRAAEKIKRENGCENRDFGFRVLRLDESNM
                     EDVYYMPEEYTQEMLYGLETNIKPDRTDLDLLFACLTEWGLLLSKPYSSEKISGYTVH
                     YYDGKELAACFNENISETVIRKIAGQHPRRAVFCDASFSDSAAKINMSELFRTISPET
                     QIKVI"
     gene            complement(4499..>5037)
                     /locus_tag="ACZPD4_04560"
     CDS             complement(4499..>5037)
                     /locus_tag="ACZPD4_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378377.1"
                     /translation="YPKLKRRESDPGYCLDDYCREVQERFSEFDGCKGENLRRRVIAW
                     ECENQCCDIFVRVYLDRPAKKGFVIGWMQKEELLDDTVQFKRMRQKNKSELALYFAKN
                     LGETKGIDCLAQAFGKPKQRVYANPYTPTNFYHKTDDCKFIRRVLKEELLIFDSEEAA
                     IQNGRFINRCRECFSKDG"
     assembly_gap    5038..5047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5048..5583)
                     /locus_tag="ACZPD4_04565"
     CDS             complement(<5048..5583)
                     /locus_tag="ACZPD4_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378378.1"
                     /translation="MRLTLQNHIVCADYGQVHLDARVVGQIINYTAETWQPDRPKKER
                     ECNIEQGKIAEEITEQFIRQYYSQELSLKTYDEIRNDDFKKHAPFDFLLWKTGTVNIA
                     FIEEAIRQDIARTPNKFVKLSNVTRRLCRTLGVKIVEVKSTNIRNDLKVESDFTGDYD
                     NVKSVQKLLETIRRKDDV"
     gene            complement(5735..6592)
                     /locus_tag="ACZPD4_04570"
     CDS             complement(5735..6592)
                     /locus_tag="ACZPD4_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378379.1"
                     /translation="MNENKEKTAGNLANSVVEGYDYSVAVRMSGRAGAYSPTGGKGNA
                     FEVMYSDKKNLSNLLKPDTTTTLTNSSTARQVDLVTKKGNRIIERIQCKDTPSAKGTM
                     DTIKRVQNGQYRTTQLVGTSESAAAFNAKAPSQGVTKVMKDSGISTKDTTRVSNKFNG
                     VQSSTGIANAAKSSAKLGGAFGGGMAAVESIVNGDDLSTTTGNVASGALKGSVSGAAG
                     TAASEATMAALAAAPIPLAAKVVIGIGAGMFAGSVASDVISDVCDGVGDIVSGIVDGI
                     GDFFGSLFL"
     gene            complement(6746..7966)
                     /locus_tag="ACZPD4_04575"
     CDS             complement(6746..7966)
                     /locus_tag="ACZPD4_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3825 domain-containing protein"
                     /protein_id="HIR1378380.1"
                     /translation="MDHMIKEKFIVTDGEKVTDEIYRVTGLDIEIDQIIENISSEESN
                     IYFFDKDLSEKPENEKDVILAWIDTGIKVNGNEPLMISLLKRSDYFSGYYVGTPSYLV
                     NGMCRKTLYSEKKLRSNLLKFCHRYREEHAERICKVETEVKDRSEGYCEKTIFIKNEE
                     VLSNVTEAIYGRLLFPNWKSMKGLDRYIKIIGARITQLVDAGRTEYFVANKTKSAIVN
                     TGMMDLFGNDFLVLYRYYEKYKTYIAERVIESKQDYLNYGFTKEQSMMQIEPISFFDE
                     KEEVFNPKKEDFDINQNCLIHIIQERRERFPESIRSQSDSKIAGQIMNALERGIKMQR
                     RDRSFAKASYSGKSGTISWFMPLHIDAPLSEDPELVMVVRRSGDFYEIKTILSYDDEL
                     KDRITALSLYSKLW"
BASE COUNT         2189 a         2045 c         1400 g         2657 t
ORIGIN      
        1 cgaaaagaat ttctccaaaa aatctgctct ctcattcaga aaaaacttga aaaaggaaaa
       61 acagtttccg aaattgcaaa tgatcttgaa gatacggaag aaaatatttc tcatttgatc
      121 aagcaatttc atctaaatta atcttatatg cttttgtatg attaaaaagg aaaaagtcca
      181 aagttgccca aaccccatct actgcttaca atctcatgat gtgccttttg gcacttccga
      241 cgatttatac tgtaagcagc tctttgaggc taaatttata atgacagcct tccctttact
      301 atgctaaaat gtaaaagatt atgagaaaaa catatgagaa atatgaaaat gaggaggatt
      361 taaactttgg aaaacaaaaa aattatccaa gtagaagaaa aagtaccttt caagctgctc
      421 gtaccgctaa gcatacaaca tatgtttgca atgttcggtg cgtccgtact tgttccgttc
      481 gtattcggac tgaatccggc cgttgtttta tttatgaacg gaatcggaac attattgttc
      541 atcctgatta caaaaggaaa agcgcctgca tacctcggct ccagttttgc atttcttgca
      601 ccggcgggaa ttgtaattga aaaatggggt tacagctatg cactcggcgg atttgtggca
      661 gtcggattct taggatgtgt ccttgctttg atcatccgca aattcggttc caaatggatc
      721 gacgttgtac ttccccctgc cgctatggga ccggttgtcg cactgatcgg acttgaactt
      781 gccggaacag ccgcttccaa tgcaggtctt acggcatctt ccatcgatcc gaaaaatgtg
      841 atcgtatttc ttgtcacact tttaaccgct gtccttggat ctgtattgtt ccgcaaattt
      901 tttgccgtga ttccgatcct gatcgctatc attgccggat atatcgcggc tcttttatgt
      961 ggaatcgttg atttcagtaa agttgcttca gcatcatttt ttgcgcttcc gaacttctcc
     1021 acgccaaaat tcaaatggga agcaatcgtg atcattctgc ctgttatcct tgttatcgcc
     1081 tctgaacata tcggacatca gatcgtaaca agcaaaatcg tcggacgcga ccttctgaaa
     1141 gatccgggcc tgcaccgttc cctctttgca gataattttt ccacgatgat ctcaggattt
     1201 atcggctccg taccgacaac aacatacggc gaaaacatcg gagttatggc tatgacaaaa
     1261 gtttacagcg tatacgttat cgcaggcgcc gcagtactgt cgatcatctg ttctttcatt
     1321 gggaaaatga cgactttgat cagcactatc cccggccctg ttatcggcgg aatttcattc
     1381 cttctgtacg gaatgatcgg cacgtcaggt atccgcatcc ttgtcgattc tcaagttgac
     1441 tacgcaagat caagaaatat ggcaatgacc tctattattt tcgtaaccgg actatccggt
     1501 atcaaagtac agtttggaag tattcagctt acaggtatgg tactggcatg tgttgtcgga
     1561 atgatcatgg gattgatgtt cttcctcttc gataagctaa aacttacgaa tgacagagaa
     1621 aactgctaaa acacactgaa aaaacaaaaa gaaagaacag aggaataatt accatgctga
     1681 aaattgtcat ttcaccggca aaaaagatga atatcacaga tgaattcccc tgtaacgcca
     1741 ctgtcccggt cttttctgac cgggcagatg tgcttcacgg gattttaaaa aataaaaata
     1801 tacaggaact gaaaacactt tggaaatgca gtgataaact tgccgaacag aattatcaaa
     1861 gacttcatgc cttttctccc gatcaggcgg taacaccggc gcttcttgcc tatgaaggaa
     1921 ttcaatatca acacattgct ccttccgttt ttacagatgc ccagtggcat tatgtaaatg
     1981 ttcaccttcg cattttatcc ggattttacg gaatcttaaa accgacagac aaagttattc
     2041 cctatcgttt ggaaatgcag gcaaagttag aagctgctaa aaagaacgac ctttatgaat
     2101 actggagcga tacgctgtac caaagtctcc tcgcagaagg gatgaccgaa cttgtcaatc
     2161 tcgcttccgc agaatacagt aaagtaatcc ttccctacaa aaatatccgc tgcattacat
     2221 gtattttcgg cgaagaggta aacggaaaaa taaaagtcaa gggcactcag gctaagattg
     2281 cccgaggcga aatggtacgc tggatggcag atcagaaaat cgaaagcgtt tcggacatcc
     2341 gtgaattcaa agaactggga tatcgttttt ctccctctca ttccacggag gacacttaca
     2401 ctttttctct ttgatctaac tgcagctttg ttttgtatat gcggacatat cactcccatt
     2461 tgggccgcaa acgataactt gggctgctga ctataacttt gactacagac tatagttttt
     2521 ccgcagacta tgactttagt tgccgactat aactttgctc caggactctt tcatattact
     2581 tttatctgtg tctccggtga gatcgtacga aaaagctcgc tcatattgat cttcgcagca
     2641 ctgtccgaaa aacttgcgtc acaaaatact gcccggcgcg gatgctgtcc ggcaatcttt
     2701 ctgatcacag tctcggatat attttcatta aagcaggcgg ctaactcttt tccgtcataa
     2761 taatgcactg tataaccact gattttctcc gaagagtacg gctttgacaa taaaagtccc
     2821 cattccgtca gacaggcgaa caaaagatct aagtctgtac gatcaggctt aatatttgtc
     2881 tcaagtccgt aaagcatttc ctgcgtatac tcttcaggca tgtaataaac atcctccata
     2941 ttactttcat caagccgcag cacacgaaat ccaaaatctc tgttttcgca tccgttttcc
     3001 cgttttattt tttctgctgc ccggcggatc cgttcttttc cgatctcaca gattgtctta
     3061 tatcctgcct tatacgcttc acttttttta tcacattcct gcggtatctg aactaaaata
     3121 tagtgcagat ctatgttttt atccgcctca gtctgcatca ccgcatgagc cgttgtcgcc
     3181 gatcctgaaa agaaatccat tacgatatcg ccgtcttttg ccgccatatt gataaaatat
     3241 tgtatgacgt tgaccggctt tacattctca aacactcttt ttccgtcaaa cagttcctgt
     3301 acgcactgcg tacctgcatt gtatatgctg actgcctctt tcggaatcaa agtggacgga
     3361 gcacccatcg cgtctcccaa atatttttta atatagatgt tccaatgtgc ctgatctccg
     3421 ttttgatcga caagcggacc tgtcttcgtt cttttaaatg tcacaagacc ttcttccttt
     3481 gcctgctcaa acctttcccg cacgaaccgg taaatatatc cgtctttcgg acgcactctc
     3541 tccccgtccg gacagatgat ctcgtatttt tggtttgcat ttgtcgattt atttttaaac
     3601 acaaaatctt tatctctgta taatccccgc tcgtcttttt cccgatacat cttgatcgct
     3661 tcccctgaca acggcgcaaa aaacatatcc agacaagttt tattttttgc ataacacaat
     3721 atatattcat gtgtcttact cgcaaatttc ggccggatac cgcctgccgt agagcaattc
     3781 catataaagc agttgacaaa atttcgttcg ccgaaaacct cgtcacatat tttttttaaa
     3841 tttgcctgct cgttatcgtc tatactgata aaaattactc cgtcatcaca gagaaggttt
     3901 ctggcggcaa gaagccgtgc ataaatcatg ctgcaccaat ccgaatgata ccttccgctg
     3961 taatccaaat tctgatacat ccggttttta tcatcttgag aagctccgcc tgcattcaca
     4021 tattctctcg ctctgatccg aaaatcatcc gcataaataa aatcatttcc cgtattataa
     4081 ggcggatcga tatagattac ttttaccgaa tccaaataac tttcctgaag aagtttcagc
     4141 acatccagat tatcgccttc tatatagaga ttgcctgtcg aatcccactc aacagagtct
     4201 tgcactacgg gacgcagcgt ttttctgatc ggtttataaa cttcttttac ggcttcccgc
     4261 tttccgaccc atgaaaattc gcatgtttcg ctttcctttt gattgattcc caaaagaagc
     4321 tgcaacgtat tgatatcgac ttttcggatt gtcttcccat cgttatcttt ttgttctttt
     4381 acgcatgacg ggaaaatatc cgcaagcctg cggatatttt cctgaaaacc gtcaacagat
     4441 tcaaatctca tcttttccat ctatgtcttt cctcaactat ttcctgctct gccatacttc
     4501 atccgtcttt tgaaaaacat tctctgcatc ggtttataaa tcttccgttt tgaatcgccg
     4561 cttcttcact gtcaaaaatg agcagctcct ctttcagcac tcttcttata aacttgcagt
     4621 catctgtttt atgataaaaa tttgtcggcg tatatgggtt tgcatacact ctttgcttcg
     4681 gctttccaaa cgcctgcgcc aaacagtcaa taccttttgt ctctcctaag ttctttgcaa
     4741 aatacaaggc aagttctgat ttatttttct gtctcatacg cttaaattga accgtatcat
     4801 cgagcaattc ctctttctgc atccatccga tcacaaatcc tttttttgcc ggacgatcca
     4861 ggtaaactct cacaaagata tcacagcact gattttcgca ctcccatgcg atcactctgc
     4921 gccgcaggtt ttcgccctta catccgtcaa attccgaaaa cctttcctga acttccctgc
     4981 agtaatcatc cagacaatat ccaggatcac tttcccgccg tttcaacttc ggataacnnn
     5041 nnnnnnnaat acatcatctt tacgccggat cgtctccaac agtttttgca ctgacttcac
     5101 attatcataa tcccctgtga aatcactttc tactttcaaa tcgtttctta tattcgtaga
     5161 ttttacttct acgatcttca cgcctaatgt cctgcataac cgccttgtaa cattgctcag
     5221 tttcacaaat ttattaggtg ttcttgcaat atcctgcctg attgcttctt ctataaaagc
     5281 aatgttgacc gttccggttt tccagagcag aaaatcaaac ggggcatgtt ttttaaaatc
     5341 atcgtttctg atctcatcgt aagttttcaa agacaactcc tgagaataat actgacgaat
     5401 aaattgttcg gtgatctcct ccgcgatctt cccctgttct atattacatt cccgctcttt
     5461 cttcggtctg tccggctgcc aagtttccgc cgtatagttt atgatctgtc ctaccacccg
     5521 ggcatctaaa tgcacctgtc cataatccgc acatacaata tgattttgta aagtcaatct
     5581 catcttctgc tttgcacctc catgtgcacg acttcaactt ttgtatcgag atttcttttc
     5641 cttaaataca caaatcgctt acgtacaaga tctttctata tagtgtacct cattatatca
     5701 caaacggcaa tgtgcatttc aaccgaactg ttacttataa gaaaagactt ccgaaaaagt
     5761 caccgatgcc gtctacaata cctgacacaa tatcgccgac tccgtcgcac acatcgctga
     5821 taacatccga tgccacactt cccgcaaaca ttcccgcgcc gatcccgata acaacttttg
     5881 cggctaacgg aatcggtgcg gccgcaagag ccgccatcgt cgcctctgat gcggctgttc
     5941 ccgccgcccc ggaaacagat cctttcaaag cgccggacgc aacatttcct gtcgtcgttg
     6001 acagatcgtc tccgttcacg attgattcca ccgcagccat tcctcctccg aaagctcctc
     6061 cgagttttgc cgacgatttt gccgcattgg caatcccggt tgatgactgg actccgttaa
     6121 acttattgct tacacgggtt gtatctttcg ttgagattcc gctgtctttc attactttag
     6181 tcacaccttg agaaggcgcc tttgcattaa atgccgccgc actttcagac gttcctacta
     6241 actgcgttgt tcgatattga ccgttttgca cccgtttgat cgtatccatc gttccttttg
     6301 cactcggagt atccttgcac tgaatcctct ctataatgcg atttcccttc ttcgtaacca
     6361 aatctacctg acgggcagtt gacgaattcg taagcgttgt tgtcgtgtcc ggcttcagca
     6421 gattagagag attcttctta tcagaataca tcacctcaaa agcatttcct tttccgccgg
     6481 tcggcgaata tgcccctgcc cttcctgaca ttctcactgc tacagaatag tcataccctt
     6541 ctaccacact gtttgctaaa tttcctgctg ttttttcttt gttttcattc atttttcttc
     6601 ctcctctgtt gtttctttcc acatgcttca tctgattctt gtttcatttt gtccctttac
     6661 caaagccgcc tcctgccttg acatactcct tttcactgat ccaaacgtca ttctaaaatt
     6721 catacacttt cggcatccct ccttttcacc agagtttgct gtataaagat aaagcagtaa
     6781 tgcggtcttt caattcatcg tcataagaca aaattgtttt tatctcatag aagtctcccg
     6841 atcttcttac taccatgaca agttccggat cttcacttaa cggagcatct atatgcagag
     6901 gcatgaacca tgaaatagtt ccgcttttcc ctgaataaga agcttttgca aacgatctgt
     6961 cgcggcgctg cattttaatc ccccgctcaa gagcattcat gatctgacct gcgatcttgc
     7021 tgtcgctctg actgcgtatg ctttccggaa aacgttcccg cctttcctgt atgatgtgga
     7081 tcagacagtt ttgattaatg tcgaaatctt ccttcttcgg attaaacact tcttcttttt
     7141 catcgaaaaa actgattggc tcaatctgca tcattgactg ttcttttgta aatccataat
     7201 tcaaataatc ctgtttactc tcgattacac gctccgcgat atacgtcttg tacttttcat
     7261 aatatcgata tagaactaaa aaatcatttc cgaataaatc catcattccg gtatttacga
     7321 ttgccgattt cgttttattc gcgacaaaat attctgtcct tccggcatcg acaagctgcg
     7381 ttatccttgc tcctatgatt ttgatatatc tgtcaagccc tttcattgac ttccagttcg
     7441 gaaacaataa tctgccatag atcgcttctg taacattaga aagtacttct tcatttttta
     7501 taaaaatcgt tttttcgcaa tatccctcac ttctgtcttt tacttccgtc tcaaccttac
     7561 aaatacgctc cgcatgttct tccctgtatc gatgacaaaa tttcaataaa tttgatcgaa
     7621 gttttttttc agaatacaga gttttcctgc acatcccatt tactaaataa gacggcgttc
     7681 cgacataata tcctgagaaa taatcagatc gttttaagag cgagatcatc agcggctcat
     7741 ttccgttcac cttgattcct gtatcaatcc atgccagaat aacgtctttt tcattttccg
     7801 gtttttccga taaatcttta tcaaaaaaat atatatttga ctcttctgac gaaatattct
     7861 ctataatctg atctatttca atatccagtc ccgtcactct gtatatttcg tccgtcactt
     7921 tttctccatc tgttacaata aacttttcct taatcatgtg atccataagt tatcctcctc
     7981 attttatgat ataatgtaac tattcagcca taagaagcac gagtgcggtt ttacggatgg
     8041 ttcaagctga actgttacac ctctcttgtt tgtaagctta tcgtatcacc ctttcccgtt
     8101 atcatgaata actgcaagtt gacatctttt tgacaagatt attctttgtc aaatgacaaa
     8161 aaattgacaa cacaatgaca attatcatta ttgatcggag gtaaatatgt taaattacgc
     8221 tgtcatcatt caatatttaa ctcaacaact gaatattccc aacgtaagga tcgcagaatg
     8281 gcttaacctt gatccttcta t
//
LOCUS       DBMDXF010000080         8175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2334_length_8175_cov_1.657295, whole genome shotgun sequence.
ACCESSION   DBMDXF010000080 DBMDXF010000000
VERSION     DBMDXF010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_8175_cov_1.657295"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="ACZPD4_04580"
     CDS             <1..245
                     /locus_tag="ACZPD4_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1378381.1"
                     /translation="YAVRKDMRGLHIGEMLVKHCMKMGKKFGFRILQFNAVVKSNAGA
                     LKLYEKLGFVRLGTIPKGFEMNDGHYEDIIPHYHEL"
     gene            complement(404..1243)
                     /locus_tag="ACZPD4_04585"
     CDS             complement(404..1243)
                     /locus_tag="ACZPD4_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1378382.1"
                     /translation="MNKLKHFFMLTFSTLLIAFGTYFFKFTNNFTFGGITGLAVLIAK
                     TGLISAGDFNFIASMILLIIGMFILGKKFAATTAYCSILLSVSLSVLERLFPMHSPFT
                     DEPMLELCFAIALPALGSAILFNIGSSSGGTDIIAMILKKYSSFDIGKALLISDALIT
                     ASGFFIFDIETGLFSLLGLSIRSFMIDTFIESFNLSKYFNVVCDNPEPICDYIVHTLG
                     RSASVCLAKGAYSGNDKYIILTALNRMQAVHLRNFIRENDKEAFILISNTSEIIGKGF
                     HSV"
     gene            1386..2666
                     /gene="eno"
                     /locus_tag="ACZPD4_04590"
     CDS             1386..2666
                     /gene="eno"
                     /locus_tag="ACZPD4_04590"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1378383.1"
                     /translation="MDKEMLIRDIFAREILDSRGNPTIEAEVLAGENIVGRAAVPSGA
                     STGKYEAVELRDQEERYGGKGVERAVENVNSCLAKAVIGMNVFDQKEIDRALCKADGT
                     ENKSNLGANAILGVSMAAARAAAKGLNIPLYRYLGGVQAKKMPVPMMNILNGGVHADN
                     PLDIQEFMIVPVRETSFRERLRICAEIYHMLRAVLKGKGLQTGVGDEGGFAPELADTK
                     EALRTIRDAAERAGYRMGRDIMIALDVAASELYIEEKGGYIFAGEGKKGEPVVRTTEE
                     MISYYEELVEEFPIFSIEDPLDEGDWDGWKKLTKKIGNRVQLVGDDLFVTNAQRLRKG
                     IELGAANAILIKVNQIGTLSETFETIKTAQEAGYNVIVSHRSGETEDTIIADIAVAFN
                     TGQIKTGAPCRSERVAKYNRLLRIEEQIGEKEKM"
     gene            2906..3349
                     /locus_tag="ACZPD4_04595"
     CDS             2906..3349
                     /locus_tag="ACZPD4_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR1378384.1"
                     /translation="MSMEINEMQEMNERAKKALEYHKKGYNCSQAVACSFCEEFGVDE
                     ETMFRIAEGFGFGMGMMDICGAVTGMFMVMGLDNSVGNPDNGKLTKADTYKKVKEYAN
                     RFKEKNGTYYCRELKSVVDGKQRVSCDQCILDAVALTESYLANRG"
     assembly_gap    3448..3457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3458..4047)
                     /locus_tag="ACZPD4_04600"
     CDS             complement(<3458..4047)
                     /locus_tag="ACZPD4_04600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378385.1"
                     /translation="MNDKISQIEKEKQEYEEYVESFAHEIKIPIGALSLTFDNTKNYA
                     LKKETDKLYRLTEQMLYYARSESTEKDYFVKKLNLEEVIHNVILKFRHDLIDHKVIIE
                     THAIDQIVYTDEKWLTFILSQIIQNSIKYFDKQENKLTITGQDNDTNIILSIKDNGCG
                     ISSSDLSRVFEKGFTGSDRSKSNSTGVGLYLARKLS"
     gene            complement(4359..5018)
                     /locus_tag="ACZPD4_04605"
     CDS             complement(4359..5018)
                     /locus_tag="ACZPD4_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378386.1"
                     /translation="MKIAIIEDDKITRLELSKLLNSHGYETVLLTDFENLSEESKKYS
                     FDLILLDINLPYENGYEICRKMKKLMSVPIIFVTSRDTSADELLSIQAGGIDFITKPY
                     DTLILLEKIKRALQLSNPNNFRELTKKDCTLDLHLSVLKYEGKSIELTRNEFRILYYF
                     FMNEDKIISKEELLEKLWNDKYYLDENVLLVNMTRLKKKMKEIGIIHLLENIRGKGWK
                     L"
     gene            complement(5122..7098)
                     /locus_tag="ACZPD4_04610"
     CDS             complement(5122..7098)
                     /locus_tag="ACZPD4_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1378387.1"
                     /translation="MLGKLAYRNAKRSIKDYLIYLITITLSFSLILAFNLVASSDEVA
                     ALSSGMSTFKNVLIFVNIVIVFVVCFLINYTTKFMFEKRSKEFGTYMLLGIKKTSIAR
                     LLVTENILVGIFAFILAIPIGFVFSQFISVIIVKLLKIPKTIFIFVNFVSIGMLAVYF
                     LLIYLLVLLNLLRRIRKMTVHDFLYFEKQNETKMFHGNRKRNILFLLSIILGIAALAL
                     WASRWTLEKNGAQETLTYLIISMSILIVSMYGICATCADMLLSSLLKSKKIKYKKDYL
                     FTARTFASKARTMSFTFGTLSMLILLSLLCLNYSSICKGVYHRSIELTAPYDVDIFDY
                     EQPFDDFNEYLRVIDEDYTINESIEFNIYKDPAHQIQNYYDVQFYDFDPVMKLSDYNK
                     LLKMRSLTPIELKSDEYFLVTDRQLLYKVKGNNEIQNIRFADQKLHLKGIDTNSFWYT
                     MNNTGRFTVIVPDKYVSGLEISEKHLIADTKEDTTSKLEEKIKTDLNHLLVTKNDDNE
                     TIREYYRVNVRGTAVEEQNTMTAMIASLCLYIAFILISAVGTILAIQSLSDSAKYKYR
                     YTTLQRLGVNDSSIFRTIRRQLLIFFFVPVICPVIAGFCMLASMNNVYHIMLESRYTY
                     LLYFISGLAIFFFIYGIYWITAYIGFKRNINEES"
     gene            complement(7088..7858)
                     /locus_tag="ACZPD4_04615"
     CDS             complement(7088..7858)
                     /locus_tag="ACZPD4_04615"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378388.1"
                     /translation="MEHILKLEQVKKYYGGAAGNITKAVDGISMEVNKGEFTAIMGAS
                     GSGKTTLLNCISTIDTVTAGHITVNHQDITKIKEKDFASFRRKNLGFIFQDFNLLDTL
                     TIEENISLSLIINKANPSSIERRVHAIADKLGIRDILSKFPYEVSGGQKQRCACARAL
                     INEPNLILADEPTGALDSRSSRLLLETMSDINRKMHTTILMVTHDPFSASFCERILFL
                     KDGKLFNEIFKGEKSRKEFFNEILDILTLLGGELGNVR"
     gene            8076..>8175
                     /locus_tag="ACZPD4_04620"
     CDS             8076..>8175
                     /locus_tag="ACZPD4_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1378389.1"
                     /translation="MIEVWHNDGDQVLAYRRKNLIFVFNFNPTKSFT"
BASE COUNT         2507 a         1376 c         1788 g         2494 t
ORIGIN      
        1 gttatgctgt ccgaaaagat atgcggggac ttcatatcgg ggaaatgctt gtaaaacatt
       61 gcatgaaaat gggaaaaaaa ttcggctttc gcattttaca gtttaatgcg gttgtgaaaa
      121 gcaatgcagg agctttgaaa ctttatgaaa agcttggttt tgtccgttta ggaacgattc
      181 caaaagggtt tgaaatgaat gacggacatt atgaagatat tattccgcat tatcatgaat
      241 tataattaat gatatgtatt gtaaggaggc tgtaattctg acaagaattt aattgttaga
      301 attacagcct cctgttgtaa cggcgacgag acaaagtccg ggttttccta aggctttgcc
      361 agccgcttat gattttggaa tgcttttttg cgcgttttgt gatttatacg ctgtgaaagc
      421 cttttccgat gatctcgctt gtgtttgaga taaggatgaa agcttcttta tcattttcac
      481 ggataaagtt gcgaagatgc acagcctgca ttctgtttaa agcagtgagg atgatgtatt
      541 tatcatttcc tgaatacgct cccttggcga gacacacgct ggcgcttcgt ccgagcgtat
      601 gtacgatgta atcacagatc ggctcagggt tatcgcatac gacgttaaaa tatttcgaca
      661 ggttaaagct ttcgataaat gtgtcgatca tgaaagaacg aatagaaagt ccgagcaggg
      721 aaaacagtcc ggtttctata tcgaaaataa aaaatccgga tgccgttatc aatgcatctg
      781 aaatcaataa tgccttgccg atgtcgaagc tggaatattt tttcaggatc attgcgatga
      841 tatctgttcc gccgctggaa gaaccgatgt taaaaagaat cgccgatcca agcgccggaa
      901 gagctatggc aaaacataat tcaagcatcg gttcatcggt aaaaggagag tgcataggaa
      961 agagccgttc cagcaccgag agcgaaacgg agagaaggat gctgcagtag gcggtagttg
     1021 cggcgaattt tttcccgagg atgaacattc cgatgatgag cagaatcatg cttgcgataa
     1081 agttaaagtc acctgccgaa atgagtccgg ttttagcgat aagaacggca agaccggtaa
     1141 ttccgccaaa agtgaaatta ttcgtaaatt taaaaaagta tgttccgaat gcaataagaa
     1201 gcgtgctgaa tgtaagcata aagaaatgtt ttaatttatt cattttattt tctccaaagt
     1261 ataaaattaa aaactctact cgaattgtaa tcttctaaaa ataaaaagtc aacgtattaa
     1321 gtataaaaat gaggagataa tgtattaata aaagaataga aagaaaataa ataaggagac
     1381 agtatatgga taaagagatg ttgattcggg atatatttgc aagggaaata ttagattcac
     1441 ggggaaatcc tacgattgag gctgaagttc tcgcagggga aaatatcgtc ggcagagccg
     1501 cagttccatc cggcgcttca acaggaaaat atgaggctgt ggagcttcgg gatcaggaag
     1561 agcgttacgg aggaaaagga gtggagcggg cagttgaaaa tgtgaacagt tgtctggcaa
     1621 aagcggtgat aggaatgaac gtgttcgatc aaaaggagat cgaccgtgct ctttgtaaag
     1681 cggacggaac agaaaataaa agcaacctcg gtgcaaacgc gattttagga gtgtccatgg
     1741 cggcggcaag agcggcggcg aaaggactga atattccttt atacagatat ttgggagggg
     1801 ttcaggcgaa gaagatgccg gttccgatga tgaatatttt aaatggcgga gtacatgccg
     1861 ataacccgtt agacatacag gagtttatga tcgttccggt aagagagact tcttttcggg
     1921 agagacttcg tatttgtgcg gaaatctatc atatgctaag agcggttctg aaaggaaaag
     1981 gtcttcagac aggagttggg gatgaaggcg gatttgcgcc tgaacttgca gatacgaaag
     2041 aagcgcttcg gacgataagg gatgcggcag aaagggcagg ataccgtatg gggcgggata
     2101 ttatgatcgc gcttgatgtg gcggcgtctg agctgtatat tgaagaaaaa ggcggatata
     2161 tttttgcggg agaagggaaa aaaggagaac ctgtcgtgcg gacgacagaa gagatgatct
     2221 cttattatga ggaacttgtt gaagaatttc cgattttttc gatcgaagat ccgcttgatg
     2281 aaggagattg ggacgggtgg aaaaagctta cgaaaaagat tggaaacaga gtccagcttg
     2341 taggagatga tctttttgtc acaaatgcgc agagattgag aaaaggaatc gaacttggag
     2401 ccgctaatgc gatactcata aaagttaatc agatcggtac tttaagtgaa acgttcgaga
     2461 cgataaaaac agctcaggaa gcaggttata atgtaattgt atcgcatcgt tccggagaaa
     2521 cagaagatac gatcatcgct gatatcgccg tggcgtttaa taccgggcag atcaaaacag
     2581 gagcgccatg ccggtctgag agagtggcaa aatacaacag actcttacgg atcgaagaac
     2641 agatcggtga gaaagaaaag atgtaggaac gatatgcagc gagcggcgga agaagcgtaa
     2701 gagacgtgta gtgagggctg tgcaggtgaa gatacgggaa tggttgcata tcttttcttc
     2761 acctgttata cttttacgcg gaagccatct ggcaaaacgc cgttttgatc tgatatttta
     2821 ttgggaggat gacgcaggct atggcgataa acaaagttgg aaatgagttt gaaaagaaca
     2881 agataagata aaaaaggaga aaaggatgag tatggaaatc aatgaaatgc aagaaatgaa
     2941 tgaaagagca aagaaagctt tggaatatca taagaaagga tataattgct cgcaagcagt
     3001 ggcatgttct ttttgtgagg agtttggagt ggatgaagaa acgatgtttc gtattgctga
     3061 aggttttggt ttcggtatgg gcatgatgga catatgcggt gcggttactg gtatgtttat
     3121 ggtgatggga ctggataata gcgttggaaa tcctgacaac ggaaaactta caaaagcaga
     3181 tacatataag aaagtaaaag aatacgccaa tcggtttaaa gagaaaaacg gaacatatta
     3241 ctgccgtgaa ctcaaaagcg ttgtggacgg aaagcaaaga gtaagctgcg accaatgcat
     3301 tttagatgcg gttgcgctga cggaaagcta cctggcaaac agaggataga ttttcagatc
     3361 cggtgaaccg ttcctttcgg aaaagtgatc tgtaatatgg tatattcttt ttcttcggat
     3421 gcaatatcta attttagtcc cagacgannn nnnnnnnaag gaaagttttc gggcgagata
     3481 taaccctacg cctgttgagt tcgatttgct ccggtcagat ccggtaaacc ctttttcaaa
     3541 tactcttgac aggtcggaag aggagatgcc gcagccgttg tccttgatcg aaaggataat
     3601 atttgtgtcg ttatcctgac cggttatcgt aagtttattt tcttgtttgt caaagtattt
     3661 gatcgaattt tgtatgatct gtgacagtat gaaagtcagc catttctcat ctgtgtagac
     3721 gatctggtca atcgcatgag tttctattat gactttgtga tcgatcagat catggcggaa
     3781 ctttaaaatg acgttgtgta tgacttcttc taagttcagt ttttttacaa aataatcttt
     3841 ttctgtactt tcgcttcgcg cataataaag catctgttct gtaaggcgat agagtttgtc
     3901 ggtttctttt ttcagcgcat agttttttgt gttgtcaaaa gtcaaagata acgcgccgat
     3961 cggaattttg atctcatgcg cgaagctttc tacatattct tcgtattctt gtttttcttt
     4021 ttcgatttgt gagattttgt cattcatagc tttgcacgct ttttttaacg cataatagta
     4081 ggctttgttc tgaaggtcgc ccggctttgg caaaatatct gagatataat agctctcttg
     4141 aagctcatct gcaagattaa taatgcgttg tgaatctttt tgtttctttt gaaatgaaaa
     4201 ccatagaaaa gagatttgcg ccgaaagaaa aaggactgcc gataaaataa taaatgatga
     4261 atctacatcg tagaaaatca agagaaatac aatgcctgaa agaaagaaaa tttgaaataa
     4321 taattcggat attttccctt ctaaaaatgt caaaaagctc acaacttcca cccctttcct
     4381 cttatatttt ctaatagatg aataattccg atttctttca ttttcttttt taaccgggtc
     4441 atattaacaa gcagtacgtt ttcatctaaa taatatttat cgttccatag tttttctaat
     4501 agctcttctt tactgatgat cttatcttcg ttcatgaaaa aataatataa aatgcgaaat
     4561 tcgttccttg tcagctcgat actttttcct tcgtatttta atacggacaa atgaagatcg
     4621 agagtgcagt ctttttttgt aagttcccgg aaattattcg gatttgataa ttgaagtgcc
     4681 cgtttgattt tctccaggag aataagtgtg tcatatggtt tcgtgataaa atcgatccct
     4741 cctgcctgaa tacttagcag ttcgtcggcg cttgtgtccc tgcttgtcac aaaaataatc
     4801 ggtacagaca tgagcttttt catcttcctg catatttcat atccgttttc gtatggaaga
     4861 ttaatatcca gtaagatcag atcgaaagaa tatttttttg attcttctga taaattctcg
     4921 aaatctgtta ataaaacagt ttcatatccg tgcgagttca gtaatttgga aagttcgaga
     4981 cgggtgattt tgtcatcttc tataattgca attttcattt tccgtacctc ttgatttgct
     5041 ttttgtaatt gcggtgatcc ttttgcttag tattcctgat ttattattat aataaaatta
     5101 cggcgggaga gccagaaaaa tctaactctc ctcattgata tttcgtttaa agccgatgta
     5161 agccgtgatc cagtaaatac cgtatatgaa aaagaaaatc gctaatccgc ttataaaata
     5221 gagcaggtaa gtatatctgc tttccagcat gatatgataa acattattca tagaagctaa
     5281 catacaaaat ccggcaatca caggacaaat gacaggtaca aagaaaaaga taagtaattg
     5341 tctgcggatc gtccgaaaaa tagaagaatc gtttacgccg agcctttgca gtgtcgtgta
     5401 gcggtattta tattttgcag agtcgctcaa cgattgtatc gcaagaattg tacctactgc
     5461 agagatcagg atgaaagcaa tatacaggca gaggcttgcg atcattgcag tcattgtatt
     5521 ttgttcctcg acagcagtac ctcttacatt gactctgtaa tattcgcgga ttgtctcgtt
     5581 gtcatcattt tttgtgacga gcagatggtt taaatccgtt tttatttttt cttccaattt
     5641 agatgttgta tcttctttcg tgtctgcgat cagatgcttt tcgcttattt ctaatccgct
     5701 gacatatttg tcgggaacaa ttaccgtaaa tctgcctgta ttattcatag tataccaaaa
     5761 ggagtttgta tcgattcctt ttaaatgcag cttttgatct gcgaaccgga tattttggat
     5821 ctcgttgtta cctttcactt tatataacaa ctggcggtcg gtgacaagga aatattcgtc
     5881 gctttttagc tcgatcggag ttaagctgcg catttttaat aatttattat agtcgcttaa
     5941 tttcattacc ggatcaaaat cataaaattg aacatcgtag taattttgta tttgatgggc
     6001 aggatctttg taaatattaa attcgattga ttcgtttatc gtataatctt catctatcac
     6061 acgaagatat tcgttaaaat cgtcaaaagg ctgttcataa tcgaaaatat caacgtcgta
     6121 aggagctgtt aattcaatac ttctatgata gactcctttg catatgcttg aataatttaa
     6181 acataataaa gaaagcagga tcagcataga aagagttccg aacgtaaaac tcattgtccg
     6241 cgcctttgac gcaaacgttc tggctgtaaa aaggtaatct tttttgtatt ttattttttt
     6301 gcttttcaat aaagaggaca ataacatgtc tgcgcatgtg gcacaaatgc cgtacatact
     6361 tacgatcagt atcgacatac tgataattaa ataagtgagc gtttcctgtg ctccgttttt
     6421 ttccagtgtc caccgggaag cccaaagggc aagagcggca attccgagta tgatactcaa
     6481 tagaaataaa atgtttcgtt ttctgtttcc gtgaaacatt ttcgtttcat tttgcttttc
     6541 aaagtaaaga aaatcgtgta cagtcatttt ccttattcgt cttaacagat ttagaagaac
     6601 taaaaggtag atcagcagaa agtaaacagc cagcattccg atagacacaa agtttacaaa
     6661 gatgaagatt gttttcggta ttttgagcag ttttacgatg attacagata taaactgact
     6721 gaatacaaag ccgataggga ttgccaaaat aaatgcaaag attcctacta atatattttc
     6781 cgttacaagc agacgggcga ttgatgtttt tttgataccg agcagcatat atgttccgaa
     6841 ttctttgctt cttttttcaa acataaattt cgtcgtgtag ttgattaaaa aacatactac
     6901 aaatacgatg acgatattta cgaagatcaa tacattttta aatgtgctca ttccggatga
     6961 cagcgctgca acctcatcag aacttgccac aagattaaac gcaagtatta aagaaaaaga
     7021 aagtgttatt gtaatcagat agatcaaata atctttgata cttctttttg cgtttcgata
     7081 agccagttta cctaacattt cccaattcac ctccgagcaa ggtcaatata tccaatattt
     7141 cattgaaaaa ttcttttctg cttttttctc ctttgaatat ttcgttgaat aattttccgt
     7201 cttttaaaaa caggatacgc tcgcaaaaag aggcgctgaa cggatcatga gttaccatta
     7261 aaattgtcgt atgcattttc cggttaatat cagacattgt ttccagtaat aatctcgatg
     7321 aacgggaatc taaagcaccg gtaggttcat cggctaagat tagattcggt tcgttgatca
     7381 atgctcttgc gcaggcacag cgctgcttct gaccgccgga aacttcgtaa ggaaatttcg
     7441 ataaaatatc gcggatacct aatttgtcgg caatcgcatg gacacgccgc tcgatcgaag
     7501 acgggttcgc tttattgatg atgagcgaga gagaaatatt ttcttctata gttaatgtgt
     7561 ctaaaaggtt aaagtcctga aagataaatc ccagattctt tcgtcggaat gaggcaaagt
     7621 ctttttcttt gatttttgta atatcctgat gattgacagt aatgtgtcct gcggttacgg
     7681 tgtcgatcgt ggaaatacag tttagaagcg ttgttttgcc tgatccgctt gcacccatga
     7741 tcgcagtaaa ttctcctttg tttacttcca tggaaatgcc gtctaccgct ttcgtaatat
     7801 ttccggcagc tccgccgtaa tattttttga cttgttccag ttttaatata tgttccataa
     7861 gaatactcct ttcggcttct gtctgtttag attataacaa tcaacggcgc gttatacact
     7921 tacagttttg aaagtaagag atcagaaata ctcacaaaat attcaaaaaa atggcattga
     7981 aaacttatct tgacgattgt ataatagcaa agtgatagtt tgaaaaacgt gagttgtcac
     8041 gggaagggct atgcgaaaaa tattcaaaaa ggagagtgat tgaagtatgg cataacgacg
     8101 gtgaccaagt gttggcttac cggcgtaaga atttgatttt tgtatttaat ttcaatccta
     8161 ccaagtcatt tacag
//
LOCUS       DBMDXF010000081         8162 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2338_length_8162_cov_1.605632, whole genome shotgun sequence.
ACCESSION   DBMDXF010000081 DBMDXF010000000
VERSION     DBMDXF010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_8162_cov_1.605632"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(54..1109)
                     /locus_tag="ACZPD4_04625"
     CDS             complement(54..1109)
                     /locus_tag="ACZPD4_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR1378390.1"
                     /translation="MKNSEAILVTPKHFEIQECPVPEPKDHEILMKVEYVGMCGSDIH
                     GFEFGPFIPPKDPNQKIGLGHEVAGEVVKIGAKVTKFKVGDKVLIEPGVPDDTCEYCR
                     TGRYNICPGVDFMATQPNYKGALTQYMTHPEEWTYHIPEGMTTMEGALVEPAAVGMHA
                     AVLGGAQPGKSIVILGGGTIGLMVLQACISLGATDITVVDVIEKRLELAKKLGAARVI
                     NGKEEDTVAVLRSEEMYGDHGVDLVFEAAGAVFTAQQAVQIVSRGGKIMMVGTQSKPV
                     PIDFLKINREVTIQTSFRYCNNFPQTIEAISSGRFNVKDMVTNIYDYEDVQKAFEEAI
                     DPVKKTEMVKGVIKVAE"
     gene            complement(1202..2602)
                     /locus_tag="ACZPD4_04630"
     CDS             complement(1202..2602)
                     /locus_tag="ACZPD4_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1378391.1"
                     /translation="MRKERELIMETRKSANIGARLDRLPNSGWHIKMWLVTAFALLVC
                     WSNGIGGSVQNILLNELHWLEPGTKLLAMWGTTYTAGQLFGALVGGPIGDKIGRKKSI
                     LLYEIIHIIAMIGGAIAPNIYVLYVFRLVQGFGLGALLVVLFAGFTEYVPGRNRGVWS
                     SRNSFIGNWAHPICNGIAFLIVTTGISYNMNWRIQYMIPSVLSIIASIIIAKKLPESP
                     RWLEAQGRVDEADAILTKIEKEIEASTGKPLPPVTEEPKEVKQLPYSALFKGKLLRRT
                     IVGSLVLIGMNTIQYTLMTWMPSLLKSMGYDTSQSQFMTMFGLFGAPFGIFIASLIMD
                     KIPRRVMGVILLAAMAVLGVITGQQTTMTALIVTTFLLNTAIYMYVCYASAVYVPEMW
                     PTSAKLSGSGFCNAIGRVSNIFFPFLVTSVAAGSMGSTGVFVLISVVAVIIGVCILIF
                     GVETRGESVEDIGNVD"
     gene            complement(2654..3340)
                     /locus_tag="ACZPD4_04635"
     CDS             complement(2654..3340)
                     /locus_tag="ACZPD4_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR1378392.1"
                     /translation="MEKKLMLAPSMGCCDLYDFGHQVKFIDEHADFLHIDIKDGNYVK
                     TFGVGPDFMTVLKKVVKKPMDAHLMVKHPQDYIEACAAAGAAYITPHTDCIEGDAFVT
                     INKIISLGCKAGIAMNPAAPLEAIRYYLPLLSKVTIMIVDAGYAGQKVITQMYDKIRT
                     LAKWREEMKLDFLIEADGSMNAGVYAPLYEAGADMVVLGPPALWNKDEDIEKAWAIME
                     QEVKSELAGM"
     gene            complement(3358..4395)
                     /locus_tag="ACZPD4_04640"
     CDS             complement(3358..4395)
                     /locus_tag="ACZPD4_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR1378393.1"
                     /translation="MIDVPKPTCGPEDIIIEIKAAAICGADMKHYKVDNGSDEFNSIR
                     GHEFAGEIVEVGEKVTDWKVGQRVVSDNSAHVCGVCPACEQGDFLCCESKVNLGLDNN
                     TWGGGFSKYCKVPGEILRIHKHAIWEIPENVKYEEAAVLDPICNSYKAIAQQSHLLPG
                     QDVVVFGTGPLGLFAVQVARIMGAVNIVMVGLDEDVPVRFPVAKELGATHCVNGSKED
                     VVKRCTEICGRDNLGLVVECSGANIALKQAIEMLRPNGEVVRVGMGFKPLEFSINDIT
                     SWNKSIIGHMAYDSTSWRNAIRLLASGQIKVQPMITHRLGLSEWEKGFELMAKKEAIK
                     VIFTYDFDDED"
     gene            complement(4869..5813)
                     /locus_tag="ACZPD4_04645"
     CDS             complement(4869..5813)
                     /locus_tag="ACZPD4_04645"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIR1378394.1"
                     /translation="MNYCFGVDIGGTTVKIGLFEETGTIVEKWEIPTNTLADGEAILP
                     DIAFSLKTKIEERKLSEEDILGIGVGVPAPVTAEGIVNGSANLGWKYKEVKKEMEELT
                     GLKAYIGNDANVAALGEMWKGGGAGEKNMIMVTLGTGVGGGVIIDGKMLVGNNGAGGE
                     IGHICVNYEENDRCGCGNKGCLEQYASATGIVRLAEQKLKTEKRETILNKAAITAKDV
                     FDAVKAGDEVADEIAAEFGKYLGYGLANLAAVVNPAVFVIGGGVSKAGEVLIPYIEKP
                     YKERAFFADKNVRFVIAQLGNDAGICGSAKLVLDEKKN"
     gene            complement(5839..6768)
                     /gene="hprK"
                     /locus_tag="ACZPD4_04650"
     CDS             complement(5839..6768)
                     /gene="hprK"
                     /locus_tag="ACZPD4_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1378395.1"
                     /translation="MGRGIKLSEIAERMDLKNLTPELDLTEKEVHVPDVNRPALQLAG
                     FFDHFDSNRVQIIGNVEYTYMQTLTEERRVYIYEMLLKHPIPCMVFCRGIEPGDVFLK
                     KAVESNVPVFSASKPTSDFSAEIIRWMNVKLAPCISIHGVLVDVYGVGVLIMGESGIG
                     KSEAALELIKRGHRLVTDDVVEIRKVSDETLVGSAPDVTKNFIELRGIGIVDVKSMFG
                     VQSVRETQNIDLVITLEDWSREKEYDRLGLEEEYTEFLGNKVVCHSIPIRPGRNLAII
                     VESAAVNHRQKQMGYNAAQELYKRVQENLAKKR"
     gene            complement(6858..>8162)
                     /gene="uvrC"
                     /locus_tag="ACZPD4_04655"
     CDS             complement(6858..>8162)
                     /gene="uvrC"
                     /locus_tag="ACZPD4_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR1378396.1"
                     /translation="QGYISQEDYGAAIVEVLKFLNGNYDVILKELEGKMNTASENLEF
                     ERAIEYRELINSVKKIAQKQKITDSSGEDRDILAAASEGEDAVVQVFFIRGGRLIGRD
                     HFYLRISSGEKTSGILDSFIKQYYAGTPYIPGELMIQEEIKDRELIETWLTSKRGQKV
                     TVKVPKKGTKEKLVELASDNAKLVLSKDKERMRREEGRTIGAVKEIEALLGISQIRRM
                     EAYDISNTNGFESVGSMVVYERGKPKRNDYRKFKIKGIQGADDYGSMREVLTRRFTHG
                     LKEREENGELGKFTVFPDLILMDGGKGQVNVALQVLEDLNLNIPVCGMVKDDFHRTRG
                     LYFQNEEISIEKSSEAFRLITRIQDEAHRFAIEYHRQLRGKGQVHSILDDIEGIGPAR
                     RKALMRHYLSLDAIKAASIEELAKLPSMNEKAAQAVYNFFHT"
BASE COUNT         2009 a         2153 c         1353 g         2647 t
ORIGIN      
        1 gcttggattt atgcactttc atatcgcccg agtcggatag attcacaatt ttactattct
       61 gctactttga tcacgccttt aaccatctcc gtctttttca caggatcgat cgcttcttca
      121 aatgccttct gcacgtcttc ataatcataa atgttcgtta ccatatcttt tacattaaat
      181 cttcctgatg agatcgcttc gatcgtctgc gggaagttat tgcaataacg gaatgatgtc
      241 tgaatcgtca cttctcggtt aatctttaag aaatcgatcg gaaccggctt cgactgtgtt
      301 cctaccatca tgattttacc gccccggctt acgatctgta ctgcctgctg tgccgtaaat
      361 acagctcccg ccgcctcaaa tacgagatcc acgccatgat ctccgtacat ttcttccgat
      421 ctaagaaccg caaccgtatc ttcttctttt ccgttgatta ctcttgcagc acctaatttt
      481 tttgcaagtt ccagacgttt ttcaataaca tctacaactg taatatctgt tgccccgagg
      541 ctgatacaag cctgaagaac catcagaccg attgttcctc ctccgaggat gacgatactt
      601 ttacccggct gtgctcctcc gagaacagca gcatgcattc caactgccgc cggctctaca
      661 agagcccctt ccatcgttgt catcccttcc ggaatatgat atgtccattc ttccggatga
      721 gtcatatact gtgtcagcgc ccctttatag ttcggctgtg ttgccataaa gtcaactccc
      781 ggacaaatat tataacggcc tgtacggcaa tactcacatg tatcatccgg aacacccggc
      841 tcaataagca ctttgtctcc gactttaaat tttgttactt tcgctcctat tttaacaact
      901 tctcccgcca cctcatgtcc gagaccgatc ttttgattcg gatctttcgg aggaataaac
      961 ggtccaaatt caaatccatg aatatcagat ccgcacattc cgacatactc tactttcata
     1021 agaatttcat gatctttcgg ctccggaacc ggacattcct gtatttcaaa atgttttgga
     1081 gtcactaaga ttgcttctga atttttcatt ttatatcctt cctttcacct ttcatctttt
     1141 acaatttttc ctgttcccca atactaggag aacaggaaac tcatttacct attacttctg
     1201 tttaatcaac atttccgata tcttctacag attctcctct agtctctacg ccgaagatca
     1261 aaatacatac tccgattatt acggcaacaa cagagatcag gacgaatact cccgtgcttc
     1321 ccatagaacc tgctgcaacg cttgttacga ggaacgggaa gaagatattg ctgacacgtc
     1381 cgatcgcatt acagaagccg gaaccggaca gttttgccga cgtaggccac atttccggaa
     1441 catacaccgc ggaagcatag catacgtaca tataaattgc cgtgttcagc aagaatgttg
     1501 ttacgatcaa tgctgtcatt gttgtctgct gtcctgtaat aactccaagt acagccattg
     1561 ctgccagaag aatcacgccc ataactcttc tcggaatctt atccatgatc agtgatgcga
     1621 taaagattcc aaacggagcg ccgaacagtc cgaacattgt cataaactgt gactgtgatg
     1681 tatcatatcc cattgatttc aacaaggacg gcatccatgt catcaatgta tactggatcg
     1741 tgttcatacc gatcaatacg agagaaccta cgatcgttct tctcaaaagt ttgcctttaa
     1801 acagagccga gtacggaagc tgttttactt ctttcggctc ttctgttaca ggcggaagcg
     1861 gttttcctgt tgacgcttca atttcttttt caatctttgt aaggattgcg tctgcttcgt
     1921 caactcttcc ctgtgcctct aaccaacgcg gagactccgg aagcttcttt gcaataatga
     1981 tggatgcaat aatagaaaga acagacggga tcatatactg gattctccag ttcatattat
     2041 agctgattcc tgttgtcacg atcaaaaatg caattccgtt acagatcggg tgagcccagt
     2101 ttccgataaa ggagttacgg cttgaccata caccacggtt tcttcccggc acatactctg
     2161 taaatcctgc aaacagaact acgagaagcg ctccgagtcc gaatccctgt acaagtctga
     2221 atacatacaa tacataaatg ttcggtgcga tcgcgccgcc gatcatggcg ataatatgga
     2281 taatttcata taaaagaata ctttttttac gtcctatttt atctccgata ggacctccga
     2341 caagcgctcc gaaaagctgt cctgccgtat aagttgttcc ccacattgca aggagttttg
     2401 tacccggttc taaccaatga agctcgttaa gcaaaatatt ctgaaccgat cctcctattc
     2461 cgtttgacca gcacaccaac aaggcaaatg ctgtaacaag ccacatttta atatgccatc
     2521 ccgaattcgg aaggcgatcg agtcttgcgc cgatatttgc gctttttctt gtttccataa
     2581 ttaattccct ttcttttctc attctttgtg acagcctctt tttctatgta catgaaactg
     2641 tctgtattgt attttacatt ccggcgagtt cactttttac ttcctgttcc atgattgccc
     2701 acgccttttc gatatcctca tctttattcc aaagagccgg aggtccgagt acaaccatat
     2761 ctgcacctgc ttcataaaga ggagcataaa cgcctgcatt catcgatccg tctgcctcga
     2821 tcagaaagtc aagcttcatc tcttctctcc attttgcaag ggtacgtatt ttatcataca
     2881 tctgtgtgat caccttctgt ccggcatatc ctgcatctac gatcataatc gttactttcg
     2941 acagaagggg aaggtaatat cgaatcgcct ctaaaggagc tgccgggttc atggcaatcc
     3001 cggctttgca tcccagagag ataatcttgt tgatcgtcac aaaagcatca ccttcaatgc
     3061 aatctgtgtg cggagtgata taagctgctc cggctgcagc acatgcctca atataatctt
     3121 gcggatgttt taccattaag tgtgcatcca tcggtttttt tacaactttt ttcaatactg
     3181 tcataaaatc aggaccaacg ccaaatgttt ttacataatt tccatccttt atatcaatat
     3241 gaaggaaatc tgcatgttca tcgataaatt ttacttgatg accgaaatca tataagtcac
     3301 aacaacccat agacggtgca agcattaatt tcttttccat tgcttctccc ccggtcatca
     3361 atcctcgtca tcaaaatcat atgtaaagat tactttgatt gcttcttttt tcgccatcag
     3421 ttcaaatcct ttttcccact cggaaagtcc aagtctgtga gtgatcatcg gctgtacttt
     3481 gatctgaccg gaagcaagaa ggcggatcgc gtttctccat gatgtggagt catatgccat
     3541 atgtccgata atgctcttgt tccatgaagt aatgtcgttg attgaaaatt caagcggttt
     3601 gaatcccatt cctacacgaa caacttctcc gttcggacga agcatctcga ttgcctgttt
     3661 caaagcaatg tttgctcccg aacactcaac gacaagaccc aagttatctc ttccgcagat
     3721 ttctgtacat ctttttacta catcttcttt ggaaccgttt acgcagtgtg tagctccaag
     3781 ctcttttgca accggaaaac gaaccggaac atcctcgtca agtcctacca taacgatgtt
     3841 gaccgcgccc ataattcttg ctacctgtac tgcaaataat ccaagcggtc ctgttccgaa
     3901 tactacgaca tcctgtcccg gaaggagatg actttgctgc gcgatcgctt tgtatgaatt
     3961 acagatcgga tcaagtaccg ccgcctcttc atattttaca ttttccggga tctcccagat
     4021 cgcatgttta tggattctca ggatctctcc cggaacttta cagtatttag aaaatccgcc
     4081 gccccatgta ttattatcaa gacctaagtt tactttgctt tcacagcaga gaaaatctcc
     4141 ctgttcgcaa gccgggcaaa cgccgcatac atgagcgctg ttgtcagaca caactctttg
     4201 tcccactttc cagtccgtta ctttctcgcc aacttctacg atctcacctg caaattcatg
     4261 tccgcggatc gagttgaatt catcggagcc attatccact ttatagtgtt tcatatctgc
     4321 tccgcagatt gcagccgcct tgatctcaat aataatatct tccggaccgc atgtcggttt
     4381 cggaacgtcg atcatacgat atccgccaaa ttcttttcca tatcttgcta atctttcatc
     4441 ttttcattct cctctttttc cattctgtat tccggggttt cttttcaaat tttacttttt
     4501 tcagtcaccc gcattttcat gctctttagc ttccggccgg aaactttatt tctttatttg
     4561 tctatatttt atcatatatt tcttttaagt caacactttt tctttgtttc aaagatttta
     4621 gtctgtttac acaacatttt tttaatttta atccgttttt atcgtattaa gtattctttt
     4681 tttgtctatt ttttacattt tgattgttaa aaacaaaaat ttaaaaaaca caacatagga
     4741 taaacttatt ttttccatat cgttttatcc tatattgtgg ttttttttca atgatttcaa
     4801 ttcaaatatt tttgttttaa aaatatattc tttatttcag atatttcaaa catcaaacga
     4861 agaaaacatc aatttttctt ctcgtctaac acgagcttgg cagatccaca gattcccgca
     4921 tcatttccca actgcgcaat aacaaatctt acatttttat ctgcaaaaaa cgctctttct
     4981 ttatacggtt tctctatgta cggaatcagt acttcgccgg ctttggaaac gcctccgccg
     5041 atgacaaaca ctgccgggtt cacgactgcc gcaagattgg caagtccgta tccgagatat
     5101 tttccaaact cagccgcaat ctcatccgcc acttcgtcgc ctgcctttac ggcatcaaac
     5161 acatcttttg ccgtaatcgc agctttatta agaatcgttt cccgtttttc tgtttttaat
     5221 ttttgttctg ccaggcggac aattcccgta gcagacgcat actgttccaa acacccttta
     5281 ttcccacagc cacatcgatc gttctcttca tagtttacgc agatatgtcc gatttctcct
     5341 cctgctccgt tgttgccgac gagcatcttt ccgtcaatga tcactccgcc tcctactcct
     5401 gtgccaagcg ttaccatgat catatttttt tctccggctc cgcctccttt ccacatttca
     5461 ccgagagcgg caacattcgc atcgtttccg atgtacgctt tcaaacctgt cagctcttcc
     5521 atttcctttt tcacttcttt atatttccat ccgagatttg cgctcccgtt tacaattcct
     5581 tctgccgtca caggcgccgg aactccgact ccgatcccga ggatgtcttc ttcggatagt
     5641 tttctctctt ctatctttgt ttttaacgaa aatgcaatat ccggcaaaat tgcctctcca
     5701 tcagccaatg tattcgtcgg aatctcccat ttttcaacaa tagttcccgt ctcttcaaac
     5761 aaccctatct taactgttgt gcctccgatg tcaacgccga aacaataatt cataattcct
     5821 gatctccttc cttatctttt atcttttttt cgcaagattt tcctgcacac gtttatataa
     5881 ttcctgcgcc gcgttataac ccatctgctt ctgtctgtgg tttaccgcgg ctgactctac
     5941 gataatagca agatttcttc ccggtctgat cgggatactg tggcatacga ctttatttcc
     6001 gagaaattcc gtatactctt cttccagtcc gagcctgtca tactcttttt ctctgctcca
     6061 gtcttccagt gtaatgacaa gatcgatgtt ctgagtttct ctgacgcttt ggacaccaaa
     6121 cattgatttt acatcgacga taccgattcc tcttaactca ataaagtttt tcgtcacatc
     6181 cggcgcagag cctacaagcg tctcatcgct cactttccgt atttccacta catcatccgt
     6241 cacaagacga tgtcctctct tgatcagctc aagtgcagct tcactttttc cgatcccgct
     6301 ttctcccatg atcaaaacac cgacgccata tacatctaca agaactccgt gaatcgaaat
     6361 acacggcgca agctttacgt tcatccaacg gatgatctcc gcagaaaaat cagatgtcgg
     6421 tttggatgcg ctaaataccg gcacattact ttccaccgcc ttttttaaaa acacgtcgcc
     6481 cggctctatc ccccggcaaa ataccatgca cggaatcggg tgtttcagaa gcatttcata
     6541 aatatacact ctccgctctt ctgtcagcgt ctgcatatat gtgtactcta cattcccgat
     6601 gatctgtacg cggtttgagt caaaatgatc aaaaaagccc gctaactgaa gcgccggtct
     6661 gttgacgtcc ggcacatgga cttctttctc tgtcagatcc aactccggcg tcagattttt
     6721 cagatccatc ctttctgcga tttcacttag tttaattcct cgtcccatat attttccgtt
     6781 cctttcattt tatttttcat agccggatcg gctttcccct catccggcga cattctttat
     6841 tcatttctct tatcatatca tgtatgaaaa aaattgtata ccgcctgcgc cgccttttca
     6901 ttcatagaag gaagcttcgc aagttcttct attgaagctg ctttgatcgc gtccagactt
     6961 aaataatgac gcataagcgc ttttcttctc gcaggtccga tcccttctat atcatccagg
     7021 atggaatgta cctgaccttt tcctctgagc tgccggtggt attcgattgc aaaccgatgc
     7081 gcctcatctt gaattcgtgt aataaggcgg aaagcttcgg atgatttttc aatcgaaatc
     7141 tcttcgtttt gaaaatagag tcctctcgtc cggtgaaaat catcttttac catgccgcac
     7201 accggaatat tcagattcag gtcctctaat acctgaagcg ccacgtttac ctgacctttt
     7261 cctccatcca tcaagatcag atccggaaat acagtaaact ttccaagctc tccgttctct
     7321 tcccgttctt tcaaaccatg agtaaaacgt ctggtcagca cttctctcat acttccgtaa
     7381 tcatctgcgc cttggattcc tttgatctta aactttctgt aatcatttcg ttttggtttt
     7441 cctctctcat acacgaccat agatccaact gactcgaaac cgttcgtatt ggaaatatcg
     7501 taagcttcca tacgcctgat ctgcgaaatg ccaagcagtg cttctatttc ttttacagct
     7561 ccgattgtac ggccttcttc tcttcgcatc cgctctttgt ctttgctcaa aacaagcttc
     7621 gcattgtcag aggccaattc gaccagtttt tcttttgttc cttttttcgg aacttttacc
     7681 gtcactttct gtccacgctt tgaggtaagc cacgtttcga tcaattctct gtctttgatt
     7741 tcctcctgaa tcatcagttc tcccggaata taaggcgttc ccgcataata ctgcttaata
     7801 aaactgtcta aaataccgga tgttttttct ccgcttgaga tacgaagata aaaatgatcc
     7861 cttccgatca gccgtcctcc ccttataaaa aacacttgaa caaccgcatc ttccccttcc
     7921 gaagctgccg ccagaatatc tctgtcttct ccgctcgaat ctgtgatctt ttgtttctga
     7981 gcaatctttt tcacactgtt tatcagttcc ctgtattcta tcgcacgttc aaactccaga
     8041 ttctcagagg cggtattcat ttttccttcc aactctttca agatcacatc ataatttcca
     8101 ttcaaaaact tcaacacttc cacgatcgcc gccccgtagt cttcctgaga aatatagccc
     8161 tg
//
LOCUS       DBMDXF010000082         7851 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2440_length_7851_cov_1.551484, whole genome shotgun sequence.
ACCESSION   DBMDXF010000082 DBMDXF010000000
VERSION     DBMDXF010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2440_length_7851_cov_1.551484"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="ACZPD4_04660"
     CDS             <1..1660
                     /locus_tag="ACZPD4_04660"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR1378397.1"
                     /translation="GDRKINVPFTYETGKSGIGDERPHGVIWYWKNITITNEFKKDQS
                     IQINFEGCDYHLDLWVNGVYKGSHDGAYTRFAFELGKFAEGEEVLLSIRVEDPHSEEQ
                     VRGKQRWLSDNYGCWYVQTTGIWKDVWLEQVSDDYVEKIKITPDIYKKTVTIHTMCHS
                     NKENLIMKSDVYFEGKFINSVSGKLEAGVCQMDIDVYNKYVGEWGIELWSPDAPNLYT
                     LKISILDEDDNCKDEVESYFGMRDIRIRNGLVLLNGQPLYQRLVLDQGYWKDTGLTPE
                     NDDVLLKDIEYVKRMGFNGVRKHQKTESDRFLYWCDVHGLLVWGEGPSFYKYSDKAAK
                     NFVREWTEIVEQNYNHPCIIIWTPLNESWGVPAIERDVCQQNYSQAVYHLTKSLDSTR
                     PVISNDGWEHTMSDILTLHDYEEDGEIMRRKYLTKKEEVLSCEIYHNNFRAAYAQGFG
                     YKGEPVLISEYGGIAFEDNDKESWGYGEKVKTEEDFLERYKALTVGIKSLPYVCGYCY
                     TQLSDVQQEKNGFLTEERRFKVNPEKIRAINDLPEGVFWNKKGH"
     gene            1679..2995
                     /locus_tag="ACZPD4_04665"
     CDS             1679..2995
                     /locus_tag="ACZPD4_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1378398.1"
                     /translation="MKKRSLKIMSVVLAGVMTTGMILGGCSAGGDKENGGNKEASKEL
                     NLWMHNGQAFVDETKKLAENYEKETGVKINIQTFPYDAMSQKMKAAFTAGNEPDIMQV
                     FGAWIPTYMNQKLLAEVPEELSANFETDYLQGAVDGYAKDGTYYGVPIEMNVEYGLFY
                     NREDAAAAGVPDGPKTFDDVMKIAKNSAKFNGDVQEYGGLEFYNGDNFAALFLSWIMQ
                     NGGDFWNEDQTKFVLTSPEAKEAWQKLVDLVTVEKVTDTKHITAKMPTEQYFFANKAA
                     QLVKGSWASAVGDDLENENWKYVFMPPVKGDIPYFAVESGWGYVVSENSKNKDTAWDF
                     VKYCMEPENAKEFNLGTGTVASLKAIIDDEGYQSDERNVRVSDQYQYLQYARSVGPVQ
                     DMDFVKKTLLDTFTKAASGSIGTDEALEEMETSINNHIQELLKQAK"
     gene            3047..3943
                     /locus_tag="ACZPD4_04670"
     CDS             3047..3943
                     /locus_tag="ACZPD4_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1378399.1"
                     /translation="MEKKTSRLKRLQRRFIVCGLTPIMLLFFIFSVVPIIFSIVMSFY
                     NYNGFPGAPFVGLANYKMLFQDPEFLGALKNTVVFVLVAVVLNICISTFLAVLIKSLR
                     KKGERSFFRGWMFLPAVVPIVAVCYVWLIMFDPANGVLNQALMALGMDHPINWLNDPR
                     TALFSIVLVTLWCDLGYNLVILMSGLDNIPNMFLEAAEIDGANSIQKFFKITLPLMSR
                     NMLFVVIMTCISYFQVFAQVQIMTQGGPENHTNVIGLNIYNYAFRYSQMGYASAMAVV
                     LLGIILIVSLVQLKVGKQDWEY"
     gene            3956..4771
                     /locus_tag="ACZPD4_04675"
     CDS             3956..4771
                     /locus_tag="ACZPD4_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1378400.1"
                     /translation="MKKLVRIGKIIILAVAFIVVAFPFFWCLMLSFKSNAEIMSIPTT
                     FFPSEWIVSGYKKVLTEAPFMTWLWNSLFSSVVSTAIVVFTSCLGGYIFGKFNFKGKN
                     FLFVCVLITMMVPFQVTMIPTYIICSKLNILNSMSALIIPFMVSGFGIFLCRQFAESI
                     PNDILEAARIDGAGEWRIFFGIVIHTIKPAIAALCIFIFMGRWNDYLWPLIAIVDEDK
                     MTLPLALNFFNSSLLTDYCTSMSAGVLVMIPIIIIYFIFQKQFIEGITMTGIK"
     gene            4789..6102
                     /locus_tag="ACZPD4_04680"
     CDS             4789..6102
                     /locus_tag="ACZPD4_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HIR1378401.1"
                     /translation="MKKEFFKFVFLGAGSSVFTMRLVGDILKEDTIKKGHIALVDLDE
                     KLLRETEEAVKELVAFSGQEFEVTAHIDYKEALPGTDYLFNTIATGGYERWKSDIEVS
                     TKHGVLQSVGDTIGPGGIIRALRTIPVILDVAKTMEEICPDAWIINYANPEGAICLAL
                     QKYTKIKNFGLCHGTPDMAKQLAEEVFKVPIERFTYRAAGINHLTWFTDMKIDGKDVY
                     PKLHDKLKESGFDKKEPISKQLYDIYGLYPAPGDRHVGEFFPYYMKEEVLMEQDYEWK
                     NNDFKVVDGWREEARVLFDEVRTKKEGYEEFLKGSGETATYFIRSLATGDISDEMVNV
                     INNGYIDNVSSGIIVELPTYIDEFGLHPQKIGKLPDAIAAQCDRLGREYLLMVEAAAT
                     CNYELARQAMFLDPLVSNCKYPELLLKDLIRANLDLLPEAWEKHC"
     gene            6084..7058
                     /locus_tag="ACZPD4_04685"
     CDS             6084..7058
                     /locus_tag="ACZPD4_04685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR1378402.1"
                     /translation="MGKTLLNGGNGVFFLGIDGGGTKTAFLLINENGDIIEAKTIATV
                     SYKHVGMDNSIALLKETVHEILNDREAYICLALPNWGESKDGDGEFLSRIKEITELPV
                     KIVNDSVVGWAGSLGLSSGINLVAGTGSIAYGRNDAGEEARAGGWDERFSDEGSCYWL
                     GMKSLELFSKESDGRAEKGALLEIFRNRFELKCDFDIIDIFNRRYRNDRTKIAGLQKY
                     LLEAAQKGDLEAVKMYEIAADELAMIVGSVYRKLKFGEGTLVSYSGGLFHAGEFILGP
                     LREQLKTEGVRLCAPKYTPVEGASLLAAEMFDGEKQYIEAIRRGFDIK"
     gene            complement(7300..>7851)
                     /locus_tag="ACZPD4_04690"
     CDS             complement(7300..>7851)
                     /locus_tag="ACZPD4_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378403.1"
                     /translation="NDKQTAKETYTYENNYLVMTSKDVQNEEKGYVKLSDDHFALFTS
                     DSMILDYTTDCFVRSSAADKYDPAKDFSRYNQAWTIASGPDQFVINNETYDANELYII
                     LTEGNRLRIGKPEKIGDSNYEVLTEGLIEFSDNYNKLEFIFSDPFTGQDGTDYRSELK
                     DGQWIISPEGEIADNPQLVLSPL"
BASE COUNT         2444 a         1108 c         1945 g         2354 t
ORIGIN      
        1 aggggatcgt aaaatcaatg ttcccttcac atatgaaacg ggtaaaagtg ggatcggaga
       61 tgaaagaccg catggagtta tatggtattg gaaaaacata accataacaa atgagtttaa
      121 aaaagatcaa agtattcaaa ttaattttga ggggtgcgat tatcatttag atctttgggt
      181 gaatggagtt tataaaggat cacatgacgg agcatataca agatttgcgt ttgaacttgg
      241 aaaatttgca gaaggcgaag aagtgcttct tagcataaga gttgaagatc cgcatagtga
      301 agagcaggtt cggggaaaac aacgttggtt atctgataat tatggatgct ggtacgttca
      361 gacgactggt atatggaaag acgtatggct tgaacaggtt tctgatgatt atgtggaaaa
      421 aataaagatt acgcccgata tttataaaaa gacagttacc attcatacaa tgtgtcattc
      481 aaataaagag aatttaataa tgaaatcgga cgtttatttt gaagggaaat ttattaatag
      541 tgtttccgga aaattagaag caggtgtctg tcagatggat atagacgtat ataacaagta
      601 cgttggggag tgggggatcg aactgtggag tcctgacgct ccgaacttgt atacattaaa
      661 aatctcgatt ttagatgagg atgataactg taaagatgaa gtggaatctt attttggtat
      721 gagagatatc cgaatcagaa acggtcttgt cctattgaat gggcagcctt tgtatcagag
      781 attggttctt gatcaagggt attggaaaga taccggatta acgccggaaa acgatgacgt
      841 gctgctaaag gatatagaat atgtaaaacg gatgggattt aatggcgtga gaaaacatca
      901 gaaaacagaa agcgatcgtt ttttgtactg gtgtgatgtg cacggacttc ttgtttgggg
      961 agaaggtcca tctttctata aatattctga taaagctgcg aaaaattttg tgagagagtg
     1021 gactgagatt gttgagcaaa attataatca cccctgcatt atcatatgga ctccgctaaa
     1081 tgaatcatgg ggagtgcccg cgattgaaag agatgtttgc cagcaaaact attcgcaggc
     1141 ggtttatcat ttgacaaaat cgttggacag cacaagacct gttatttcta atgatggctg
     1201 ggaacacact atgtcggata ttttaacact tcatgattat gaagaagacg gagaaataat
     1261 gcggcgtaaa tacttaacta aaaaagaaga agtgttaagc tgcgagattt atcataataa
     1321 tttcagggca gcttatgcgc aagggtttgg ttataaagga gaacctgttc tgattagtga
     1381 gtacggaggc attgcctttg aggataatga taaagagtca tggggatacg gtgaaaaagt
     1441 aaaaacagag gaagattttc tggaaagata taaagctctt acggtaggaa tcaaatctct
     1501 gccatatgtt tgcggatact gttatacgca attatccgac gtacagcaag agaaaaacgg
     1561 ttttctgacg gaagaaagga ggtttaaggt aaatccggaa aaaataagag caattaatga
     1621 tttgcctgaa ggtgtatttt ggaacaaaaa aggacattaa aaaggaggaa gtaagaaaat
     1681 gaaaaagaga tcgttaaaga ttatgtcagt agtattggcg ggagttatga ctacgggaat
     1741 gattttaggc ggatgttctg caggcggaga caaggaaaac ggcggaaaca aagaggcgtc
     1801 taaggaattg aatttgtgga tgcataatgg acaggcattt gttgacgaga caaagaaact
     1861 tgcggagaat tatgaaaagg aaacgggagt aaagattaat attcagacat ttccttatga
     1921 tgccatgagt cagaaaatga aagcagcttt tactgccgga aatgaaccgg atattatgca
     1981 agtgtttgga gcgtggattc cgacttacat gaatcagaaa ctgctggcgg aagttccgga
     2041 agaattatct gcaaactttg agacagatta tttacagggc gctgtagatg gatatgcaaa
     2101 ggacggaact tactatggag ttccgattga gatgaatgtt gagtatggac tattctataa
     2161 tcgggaagat gcggcggcgg caggcgttcc tgacggaccg aaaacattcg acgatgtgat
     2221 gaagattgcg aaaaatagcg caaaattcaa tggggatgtt caggagtatg gcggacttga
     2281 gttttataac ggagataact ttgcggcatt attcttgtca tggattatgc aaaatggtgg
     2341 agatttttgg aatgaggatc agacaaagtt tgttctgact tctccggaag cgaaagaagc
     2401 gtggcaaaaa ttagtagatc tcgttacggt ggaaaaagta acagatacga aacacattac
     2461 tgcaaaaatg ccgacagagc agtatttctt tgcaaacaaa gcggctcaat tagtaaaagg
     2521 ttcctgggca tctgcagttg gagatgattt ggaaaatgaa aattggaaat atgtatttat
     2581 gcctcctgta aaaggcgata ttccatattt tgcagttgaa tcaggctggg gatatgttgt
     2641 ttctgaaaac agtaaaaata aagataccgc gtgggatttc gtaaaatatt gtatggaacc
     2701 tgaaaatgca aaagagttta atttgggaac aggaacagtt gcgtccttaa aagcgattat
     2761 tgatgatgag ggttatcagt cggatgaaag aaatgtaaga gtgagcgatc agtatcagta
     2821 tttacagtat gcaagaagcg taggacctgt tcaggatatg gactttgtta agaaaacttt
     2881 attagataca tttacaaaag cggcatctgg aagtattggt acagatgaag cgttggagga
     2941 aatggagaca agtatcaata accatattca ggaattattg aaacaggcaa aataagtatt
     3001 taagaggaga cgtttgtctc ctctcaaact gattggagga gtggcggtgg aaaaaaagac
     3061 aagcagatta aaacgtcttc aaagacgatt tattgtatgt ggacttactc cgataatgct
     3121 gctgtttttt atattttctg tagttcctat catattcagc attgtaatgt cattttataa
     3181 ttataatgga tttccgggag ctccctttgt gggactggcg aattataaga tgttatttca
     3241 agatccggag tttttaggag cgcttaaaaa tacagtagta tttgttttag tcgcagtagt
     3301 tttaaatatt tgtatttcca catttttagc ggtattgatt aaatcacttc gaaaaaaagg
     3361 ggaaagaagc ttttttcgtg gctggatgtt tttgccggca gtagtgccga ttgttgcagt
     3421 gtgttatgtt tggttgatta tgttcgatcc tgcgaacgga gttttaaatc aggcactgat
     3481 ggcacttgga atggatcatc cgattaactg gttaaatgat ccgagaacgg ctttgtttag
     3541 tattgtactg gttacgcttt ggtgcgattt gggatacaat ttggtcatat tgatgtcagg
     3601 gttagataat ataccgaata tgtttttaga agcggctgaa attgacgggg caaacagtat
     3661 acagaaattt tttaaaatta cattgccttt gatgtcgagg aatatgcttt ttgttgtcat
     3721 tatgacatgc atttcgtatt ttcaggtttt tgctcaggtg cagattatga cgcagggagg
     3781 accggaaaac catacgaatg tcataggatt aaatatctat aattatgcgt tcagatattc
     3841 gcagatggga tatgcatcgg cgatggcagt tgtgttgctt ggtattatat taatagtatc
     3901 attagttcag ttaaaagtcg gaaagcaaga ctgggaatac taggaggtgt cgaaggtgaa
     3961 aaagcttgtg cgaataggga aaattattat tttagcagtt gcctttatag ttgttgcatt
     4021 tccgtttttt tggtgtttga tgctgtcttt caaaagcaat gcggaaatta tgagtatacc
     4081 gacaacattt ttcccatcag aatggatcgt atccggatat aagaaagtat tgactgaagc
     4141 cccatttatg acctggctgt ggaacagttt gttttcatct gtcgtatcaa cagctatcgt
     4201 agtatttacg agttgtcttg ggggatatat ttttggtaaa tttaatttta aggggaaaaa
     4261 ttttctgttt gtttgtgtgt tgattacgat gatggttcct tttcaggtaa cgatgattcc
     4321 cacatatatc atttgcagca agttgaatat tttaaattct atgtcagcgc tgattatacc
     4381 tttcatggta tcgggatttg gaatattttt gtgtcgtcaa tttgcggaaa gcattccgaa
     4441 tgatatatta gaggcggcta ggatcgatgg ggcaggggaa tggagaatct tttttggcat
     4501 tgtaattcat acgatcaaac ctgcaatcgc ggcactgtgc atttttatat ttatgggaag
     4561 atggaatgat tatttgtggc cgttaatagc gattgtagat gaagataaaa tgacgttgcc
     4621 gctggcgttg aatttcttta attccagctt gttaacggat tactgtactt cgatgagtgc
     4681 gggtgttttg gttatgatac cgattattat tatatatttt attttccaaa agcagtttat
     4741 agaaggaatt acaatgactg gaattaaata gaaaggggac tggagagaat gaaaaaagag
     4801 ttttttaaat ttgttttttt gggtgcggga agttctgttt ttacaatgag acttgtggga
     4861 gatattctga aagaagatac tataaaaaag gggcatatcg cacttgtgga tttggatgaa
     4921 aagctgctta gagagacgga agaagctgtg aaagagcttg ttgcattttc gggtcaggaa
     4981 tttgaagtta cagcgcatat tgactataaa gaggcgctgc cgggaacgga ttatttgttt
     5041 aatacgattg caacaggagg gtacgagcgt tggaagagtg acatagaggt gtcgacaaaa
     5101 cacggagtac ttcagtcggt tggagataca ataggtccgg gcgggattat tcgagcatta
     5161 agaacgatac cggtcatcct tgatgttgct aaaacaatgg aagagatctg cccggatgca
     5221 tggataatta attatgccaa tcccgagggg gcaatctgcc ttgcgcttca gaaatataca
     5281 aaaattaaga atttcggttt atgtcatgga acgccggata tggcaaaaca acttgcagaa
     5341 gaagttttta aagttccgat agaaagattt acatatcgtg ccgcaggaat taatcattta
     5401 acgtggttta cggatatgaa gattgatggc aaagacgttt atcctaagtt acatgacaaa
     5461 ttaaaagaga gcggatttga taaaaaagaa cctatttcaa agcagttata tgacatatat
     5521 ggtttatatc cggcgcccgg tgatcgtcat gtgggagagt tttttccgta ttacatgaaa
     5581 gaagaagttc ttatggaaca ggattatgag tggaaaaaca atgattttaa agttgttgac
     5641 ggttggagag aagaggcaag agttctgttt gatgaagtaa ggacaaaaaa agagggatat
     5701 gaagaattcc tgaaaggctc aggagaaaca gcgacttatt ttattcgttc gcttgcaaca
     5761 ggagatattt ctgatgaaat ggtcaatgtg atcaacaatg gttatatcga taatgtctct
     5821 tcgggtatca ttgtagaatt acctacttat attgatgagt ttgggcttca tccgcagaaa
     5881 atcggaaaac ttccggatgc gattgcagca cagtgcgatc gccttgggag agaatatctg
     5941 ttaatggtag aagcggcggc aacatgtaat tatgagcttg cacggcaggc gatgtttttg
     6001 gatccgcttg tatctaattg caaatatccg gaattattgt taaaagatct tatccgtgca
     6061 aatcttgatt tgcttccgga agcttgggaa aaacattgtt gaatggagga aacggggtgt
     6121 tctttttagg gatagatggc ggtggaacga aaacggcctt tttgcttata aatgaaaatg
     6181 gagatattat tgaagcaaag acgattgcta cagtatctta caaacatgta ggaatggaca
     6241 attcgattgc gcttttaaaa gaaacggttc acgaaatttt gaatgaccgg gaagcgtaca
     6301 tttgtctcgc tcttcctaac tggggagagt cgaaagacgg tgatggtgaa tttttgtccc
     6361 gtattaaaga aataacagaa ctccctgtga aaattgtgaa cgatagtgtg gtggggtggg
     6421 cgggatccct cggattatct tccgggatta atctcgttgc cggcaccgga tcgattgcat
     6481 atggaagaaa tgacgcgggt gaggaagctc gtgccggcgg atgggatgaa agattttcgg
     6541 atgaaggatc atgttactgg cttggtatga aatctttaga acttttttca aaagaaagcg
     6601 atggaagggc ggaaaaaggc gctcttttgg agatttttcg gaatagattt gagttaaaat
     6661 gtgattttga tattattgat atttttaatc ggcggtaccg aaatgatcga acgaaaatag
     6721 caggccttca aaaatatttg ttagaagctg cccaaaaagg agatttggaa gctgtgaaaa
     6781 tgtatgagat tgcggcggat gagttggcaa tgatcgttgg ttccgtatac cggaagttga
     6841 aattcggcga aggaacactt gtatcttatt caggaggttt gtttcatgca ggagagttta
     6901 tattaggccc gttacgagaa cagttgaaaa cagaaggcgt ccggttatgc gcgcctaaat
     6961 atacgccggt agaaggcgcc tctttgttag cggcggagat gttcgatggt gaaaaacaat
     7021 acatagaggc gattagaaga ggatttgaca taaagtagag aggaacgttc aattatcatc
     7081 ttgaaggggc gctcgaaaac atatacaaaa aaataagaat ctcccatgga agatcaagta
     7141 tcaaaaatgt tgatttctgt aggagattct cttattttaa gatttagcag ctaaaaaaca
     7201 gtatttactg acagctcctt tttacgtggt gagcgtctgt caatcttgga acgtactttt
     7261 ggcgcttctg atgattgcaa tgatcacctt agagcatttt tacaaaggtg ataaaacaag
     7321 ctgaggattg tcggcaatct ctccttcagg ggaaatgatc cactgaccgt cttttaattc
     7381 ggatctgtag tcagttccgt cttgtcctgt aaaaggatcg ctgaaaataa attccagctt
     7441 attatagtta tctgaaaact caatcagacc ttctgtcagt acttcgtagt tagaatcgcc
     7501 gattttttca ggttttccta ttctcaatcg gtttccttct gttaaaataa tatacagttc
     7561 gttggcgtcg tatgtttcat tgttgattac aaattggtca ggaccggatg ctatagtcca
     7621 tgcctggtta tatctgctaa aatcttttgc aggatcatat ttgtcagcgg cgctgctgcg
     7681 tacaaaacaa tcagtagtgt aatctaggat catagagtca gaagtaaata acgcaaaatg
     7741 atcatctgaa agtttcacat atcctttttc ttcgttttgg acgtcttttg atgtcattac
     7801 taaatagttg ttttcgtatg tgtacgtttc tttggctgtt tgtttatcat t
//
LOCUS       DBMDXF010000083         7755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2476_length_7755_cov_1.742618, whole genome shotgun sequence.
ACCESSION   DBMDXF010000083 DBMDXF010000000
VERSION     DBMDXF010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2476_length_7755_cov_1.742618"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(38..1369)
                     /locus_tag="ACZPD4_04695"
     CDS             complement(38..1369)
                     /locus_tag="ACZPD4_04695"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR1378404.1"
                     /translation="MAAVSRENWVERIRDPKIECMSRDEMAALQSRRLVEVVNRVYNN
                     VEFYRKKMQDMGIEPGDIKSIEDIGKLPFTTKEDLRENYPFGLLAIPKKDVVRVQGTS
                     GTTGKLTIAPYSQKDVEVWGECVARELTMAGLTEEDIIHVCYGYGLFTGGLGLDFGAR
                     ALGAMAIPMSAGNTKRQMMCMEDLGATAFACTPSYALYLAESIQEAGIVDRLNLKVGI
                     HGAEPWTEEMRKKIEDILHINCFDIYGLCEITGPGVAQDCIHKAGLHVQADYFYPEVL
                     NPTTHEPCADGETGELVFTTLAKEAMPLLRYRTKDLTSIDHSTCACGRTLPRISKFTG
                     RTDDMKVIRGVNVFPTQVETALLSIGGVVAPHYMMIVDREDNLDVLTVMVEVDESVFS
                     DEIRKLDALRNKIAATLKTALGVSVKVKLVEPKSIQRSEGKAVRVIDNRDL"
     gene            complement(1402..1821)
                     /locus_tag="ACZPD4_04700"
     CDS             complement(1402..1821)
                     /locus_tag="ACZPD4_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1378405.1"
                     /translation="MIRQISVFVENQPGSMMNVTNVLTEAHVNIRAISTFDTPEFGIM
                     RLVVDDPVRAKESLTSKGFVNRVSDVIGAELKDEKGNLNQMLKILADGQINVNYIYSF
                     VIREGKAPVMVFHTDDFEKAESVLKQADVKLVEEEDL"
     gene            complement(1944..2516)
                     /locus_tag="ACZPD4_04705"
     CDS             complement(1944..2516)
                     /locus_tag="ACZPD4_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR1378406.1"
                     /translation="MAVKNIMIVGVGGQGTLLTSRILGGLTIAGGYDVKLSEVHGMAQ
                     RGGSVVTFVRYGEKVAEPIVEEGQADVIIAFERLEALRYAHFLKKDGVLIVNDWRIDP
                     MPVVIGAARYPENILEDLEKEYKVYKVDATEESKKLGNPKVFNLIVLGVAAQHMDFTK
                     EQWYEVIEKTVPQKTVELNKKAFDVGYTLK"
     gene            complement(2520..4265)
                     /gene="iorA"
                     /locus_tag="ACZPD4_04710"
     CDS             complement(2520..4265)
                     /gene="iorA"
                     /locus_tag="ACZPD4_04710"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR1378407.1"
                     /translation="MAEKVIMLGNEAIARGAYEAGVKVSSAYPGTPSTEISEYLVQYK
                     DDVYEEWAPNEKVATEVAVGASLAGVRSMACMKHVGLNVAADPLYSVSYMGVNGGLVL
                     VVADDPGLYSSQNEQDTRMVARAAQIPVIEPSDSAEAKDFFKTAFELSEQFDRPFIFR
                     TTTRLAHSQGIVELCDRAQVEDKPYEKNIPKNVMMPGNAKLRHVEIEKRNLELAEAAN
                     TLSLNKMEMNDTKIGVITSGIPYQYVKEALPDASVLKLGLVNPLPKKLIEEFASKVET
                     LYIVEELDPVIEEQVRSWGIPAVGKEIFTVQGEYSANMLRKAILKENPDLKKPAEAPG
                     RPPILCPGCPHRSVFHVLNKLKMHAAGDIGCYTLGAVAPLSVIDTTMCMGSSISTLHG
                     MEKAKGKEYIKNWVAVIGDSTFLHTGINSLMNMVYNHATGTVLILDNSTTGMTGHQDH
                     AATGKTLQGDPTYAIDIPALCRAVGVKNVYEINAFDLLLLEKTIKEEVARDEVSVIIT
                     KTPCVLLDKRKKPLYIAHADKCKKCGMCMKPGCPAMTKNPDGTIHIDDTMCTGCGLCK
                     DLCKFDAIELIKEGE"
     gene            complement(4267..5577)
                     /locus_tag="ACZPD4_04715"
     CDS             complement(4267..5577)
                     /locus_tag="ACZPD4_04715"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR1378408.1"
                     /translation="MIWAKEETLPREEIERIQLTKLKDTVRYIYDRVKPYREKMDMAG
                     VRPEDIQTLEDLKKLPFTYKADFRDHYPDGLFAVDKKEIVRYHASSGTTGKPTVVGYT
                     RNDLDIWLNNVARIACMGGATEEDVAQIAFGYGTFTGALGLHGGLEKIGASVIPMSSG
                     NTNKQIMFLQDMGVTLLVATPSYALHLGEELRRRGIDPSKDLKIRIGLFGGEGMTEPM
                     REEMHRVWGEQFVCTQNYGMSELCGPGVSGECTQLCGMHINEDWFIPEIIDPETEEVL
                     PPGERGELVVTCLGKEALPIVRYRTGDMTRLMYEPCKCGRTTCRMENLSGRADDMLVI
                     RGVNVFPTQIEEVLLKIDEIGPHYEILVERKNRLDVMTITVELIDDRLLDSYAKLSEL
                     ETRIKSALKSQLGLATCIRLVAPNSLRRFEGKAKRVTDLRKDGL"
     gene            complement(5789..7024)
                     /gene="glyA"
                     /locus_tag="ACZPD4_04720"
     CDS             complement(5789..7024)
                     /gene="glyA"
                     /locus_tag="ACZPD4_04720"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1378409.1"
                     /translation="MYDFDEIKNADPEIAEVITAEMKRQNSHIELIASENWVSKAVMA
                     AMGSPLTNKYAEGYPGKRYYGGCQCVDVAEELARERAKKLFGCEYVNVQPHSGAQANM
                     AVQFAMLTPGDTIMGMNLDHGGHLTHGSPVNLSGKYFHVVPYGVNDDGVIDYDKVLEI
                     AKECKPKMIIAGASAYARTIDFKRFREIADEVGSYLMVDMAHIAGLVAAGLHPSPIPY
                     AHVTTTTTHKTLRGPRGGMILSSNEVNEKFNFNKAIFPGTQGGPLMHVIAAKAVCFKE
                     ALEPEFKEYQMQVVKNAKALCEGLKKRGVKIVSGDTDNHLMLVDLTGNDVSGKELEKR
                     LDDAHITCNKNTIPNDPRSPFVTSGVRLGTPAVTTRGMKEDDMDKIAEIIAMVIESEE
                     NVETARKLAAELTEKYPLC"
     gene            7182..>7755
                     /locus_tag="ACZPD4_04725"
     CDS             7182..>7755
                     /locus_tag="ACZPD4_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378410.1"
                     /translation="MGEQILIAEDDADINNLLAKILRTEHFKPIQAFSGTEAKLLFMH
                     NTPDLILLDLMLPGLSGEELLSELRENLSSDVPVIILSAKIALADKVSLLKMGADDYI
                     TKPFEPEEVIARIQAALRRTATSKKLSCLNLELYPDSRKVTVHGIELSLTAHEYDLLY
                     LFIKNPEKVYSREMLYDLVWHGGYYGENNTV"
BASE COUNT         1972 a         2025 c         1307 g         2451 t
ORIGIN      
        1 ttgttgaatt gtaattccca ttcgttctct cctccttcta cagatctctg ttgtcaatca
       61 cgcgaacagc ttttccttca cttctttgaa tcgacttcgg ctctacaagc ttcactttta
      121 cagatacgcc aagcgcagtc tttaatgtag ctgcaatctt atttctgaga gcgtcaagtt
      181 ttctgatttc atctgaaaat acagattcgt ctacctctac cataacagtc agtacatcaa
      241 gattatcttc cctgtcaaca atcatcatat aatgaggcgc tacaactcct ccgattgaaa
      301 gaagcgccgt ttcaacctga gtcgggaata catttacacc gcgaattact ttcatatcgt
      361 cggttcttcc tgtaaattta gaaattctcg gcagtgttct tccacaggca caggtactgt
      421 gatcaatact tgtaagatct tttgtccgat atcttaaaag cggcattgcc tccttcgcaa
      481 gagttgtaaa cacaagttcg cctgtttctc cgtccgcaca cggttcatgg gttgtcggat
      541 tcaaaacttc aggatagaaa taatcagcct gtacatgaag tcccgcttta tggatacaat
      601 cctgagccac tcccggacct gtaatttcac aaagtccgta aatatcaaaa caattaatgt
      661 gaaggatatc ttcaatcttt ttccgcattt cttccgtcca cggttctgcg ccgtgaatac
      721 cgactttcag attcagcctg tcaacaatcc ctgcttcttg aatggattct gcaagataca
      781 aagcatacga aggcgtacac gcaaatgcag tggctccaag atcttccata cacatcatct
      841 gacgctttgt attgcctgca gacatcggaa tcgccattgc cccgagcgct cttgctccaa
      901 aatccagtcc aaggcctccg gtaaacagac cataaccata acagacatgt ataatatctt
      961 cctcggtaag tcctgccatc gtcagttctc ttgccacaca ttccccccat acttctacat
     1021 ctttctgcga ataaggagca atcgtcagtt ttcctgttgt accggatgtt ccctgcacac
     1081 ggacaacatc ctttttcggt attgccaaaa gtccaaaagg ataattttcc cttaaatctt
     1141 cttttgttgt aaacggaagt tttccgatat cttcaataga tttgatgtca ccgggctcta
     1201 ttcccatatc ctgcattttt tttcgataaa attctacatt attatatact ctgtttacta
     1261 cctcgacaag ccgtctgctc tgaagagcgg ccatctcgtc cctgctcatg cattcaatct
     1321 taggatcacg aatcctctca acccagtttt ctcttgatac tgcagccatt atttcttctc
     1381 ctttagtcaa ttatatatct tttataaatc ctcttcctcc acaagcttta catccgcctg
     1441 cttcagaacg ctctccgctt tttcaaagtc atcagtatga aataccatga ccggggcttt
     1501 cccttctcgg attacgaaag aatagatata atttacattg atctgtccgt ctgccagtat
     1561 tttcaacatc tgattcagat ttcccttttc gtcttttagt tccgcaccga tcacatcact
     1621 gacgcgattt acaaaccctt ttgacgttaa actttctttt gcgcggaccg gatcatctac
     1681 taccaaacgc attatgccaa attccggagt atcaaatgtt gaaattgcac gaatattgac
     1741 atgcgcctca gtaagtacgt ttgtcacatt catcatactt cccggttgat tttccacaaa
     1801 aactgatatc tgcctgatca ttaactatca ccgcctttcc ttttagattc tctgtcaata
     1861 tctgccaata ttttttcaca cagattgttt gttgttacga tacattcatt atcatatgtc
     1921 tttttctttt atatggcttc cacctatttc aacgtatatc ccacatcaaa agctttctta
     1981 tttaattcga ctgtcttttg gggaaccgtt ttctctatca cttcatacca ctgctctttt
     2041 gtaaaatcca tatgctgtgc cgctacgccg aggacgatca ggttaaacac tttcggattt
     2101 cccagtttct ttgattcttc tgttgcatct actttataga ctttatattc tttttccaaa
     2161 tcttctaaaa tattttccgg ataccgggct gctccgatca cgacaggcat cggatcgatt
     2221 cgccagtcat ttacgatcaa cactccgtct ttcttcaaga agtgcgcata gcgaagcgcc
     2281 tcaagccgct caaatgcaat aattacatct gcctgccctt cttccacaat cggttccgcc
     2341 actttttctc cgtatcgaac aaatgtaacg acgcttccgc ctctctgtgc cattccgtga
     2401 acttccgata atttcacatc atatccgccc gcgatcgtaa gacctccgag aatgcggctt
     2461 gtgagcaaag ttccctgtcc gcccacaccg acgatcataa tatttttcac agccatccct
     2521 tactcacctt cctttataag ctcgattgca tcaaatttgc acaaatcttt acacaaaccg
     2581 catcctgtac acattgtatc gtcaatatga atcgttccgt ccgggttttt cgtcattgcc
     2641 ggacatcccg gcttcatgca cattccacat tttttgcact tatcggcatg tgcaatataa
     2701 agcggctttt tcctcttatc aagaagtaca cacggagttt ttgtaataat gaccgatact
     2761 tcgtctcttg ccacctcttc tttgatcgtt ttttcaagca gcaaaagatc aaaagcattg
     2821 atctcataaa catttttcac gcccactgca cgacacaaag ccggaatatc aatcgcatat
     2881 gtcggatcgc cctgcaaagt tttccctgta gccgcatggt cctgatgtcc tgtcattcct
     2941 gttgtcgaat tatcaaggat cagtactgtt cctgtcgcat gattatatac catattcatc
     3001 aatgaattga tgcctgtatg caaaaaggtg gaatcgccga ttacggcaac ccaatttttt
     3061 atgtattctt ttccttttgc tttctccatt ccatgaagcg tcgaaatgct tgatcccata
     3121 cacatcgtcg tatcgattac gctcaaaggc gcaaccgctc caagcgtata acatccgatg
     3181 tcgccggcag catgcatttt cagcttattc aatacatgga aaacacttct atgcggacat
     3241 cccggacaaa gaatcggcgg tcttcccgga gcttctgccg gttttttcag atccggattt
     3301 tctttcagta tcgctttgcg aagcatattg gcactgtact cgccctgcac tgtaaaaatt
     3361 tcttttccta cagccggaat tccccatgaa cgaacttgtt cttcaattac cgggtcaagt
     3421 tcttctacga tatacagcgt ctcgactttt gatgcaaact cttcgatcaa ttttttcgga
     3481 agaggattca caagtccaag tttaagaaca gatgcatctg gaagcgcttc ttttacatat
     3541 tgataaggga taccgcttgt aataactcct atctttgtat cattcatctc catcttattc
     3601 agcgaaagtg tattcgctgc ttctgccagt tcaagattgc gtttttcaat ttcgacatga
     3661 cgaagtttcg catttcccgg catcatgaca tttttcggaa tatttttctc atacggttta
     3721 tcttcgacct gcgcacgatc acacaactct acaattccct gcgaatgtgc aagtcttgtt
     3781 gttgtcctga aaataaaggg tctgtcaaac tgttcactca attcaaacgc cgtcttaaaa
     3841 aaatcttttg cctccgcact gtccgacggt tcaatcaccg gaatctgagc ggctcttgca
     3901 accatcctcg tatcctgttc gttctgagaa ctgtaaagtc ccggatcatc tgcaacaaca
     3961 agaacaagtc ctccgtttac tcccatataa gaaaccgaat acagcggatc tgccgccaca
     4021 ttcaaaccga catgcttcat gcatgccata gaacgtactc cggcaagact tgctccgacc
     4081 gccacttctg tcgccacttt ttcattcggc gcccactctt catacacatc atctttatat
     4141 tgtacaaggt attcacttat ctcggtactc ggagttcccg gataagcaga cgataccttt
     4201 acccctgctt cgtatgctcc tctcgcaatc gcctcattgc cgagcatgat cactttctct
     4261 gccatatcaa agtccatcct ttcgtaaatc tgttactctc tttgccttac cctcaaaccg
     4321 tcttaaagag ttcggcgcca caagcctgat acaggttgca agtccaagct gcgatttcaa
     4381 tgctgactta atcctcgtct caagttcact taattttgca tagctgtcaa gaagtctgtc
     4441 atcgatcagc tctaccgtaa tcgtcattac atccagacgg ttctttctct ctacaagtat
     4501 ctcataatgc ggtccgatct catcaatctt taagagcact tcttcaatct gtgtcgggaa
     4561 tacattaact cctcggataa caagcatatc atctgcacga ccggaaagat tttccattct
     4621 gcaggttgtt cttccgcatt tacacggctc atacatcagt cttgtcatat ctccggttcg
     4681 gtatcttacg atcggaagcg cctcttttcc aagacaggta acaacaagtt ctcctctttc
     4741 tcccggcgga agaacttcct ctgtttcagg atcaataatt tcaggaataa accagtcttc
     4801 attaatatgc attccacaaa gctgtgtaca ttctcccgaa acacccggtc cgcaaagttc
     4861 actcatacca taattttgtg tacagacaaa ctgttctccc caaacacggt gcatctcttc
     4921 ccgcatcggc tctgtcatgc cttctcctcc gaacagtccg attcttatct ttaaatcttt
     4981 tgatggatcg atccctcttc tgcgaagttc ctctccaaga tggagcgcat aagacggggt
     5041 agccacaaga agtgtcacac ccatatcctg taaaaacata atctgtttgt ttgtatttcc
     5101 tgaagacatc ggaatcacag atgctccgat cttctcaaga cctccgtgaa gtccgagcgc
     5161 tcccgtaaat gttccgtatc caaacgcgat ctgtgccaca tcttcttccg tcgcgcctcc
     5221 catgcatgca attctcgcaa cattattcag ccagatatcc agatcgtttc ttgtatatcc
     5281 gacaacggtc ggctttcccg tcgtacccga acttgcatga taacgaacga tttccttttt
     5341 atcaaccgca aacaacccat ccggataatg atcccgaaaa tcagctttat atgtaaacgg
     5401 cagttttttt aaatcttcca acgtctgtat atcttccgga cgtacacctg ccatatccat
     5461 tttctcgcgg taaggtttca ctctgtcata aatatatcga accgtatcct tcagctttgt
     5521 aagctgtatc cgttcgatct cttctcttgg cagagtctct tcctttgccc atatcatcgc
     5581 gcatatcctc ctttataata tgttgtgcca tcgcttatcg gcaatctgct tttcagcttt
     5641 cttttttact gatgtctatt agtatagcac aatacttttg cgaattaaag ctttaaatca
     5701 agaaaaattc ctctttaccc ggaggacctg atcctgtcgg gcaaagagga actctattgt
     5761 ttctattctt tttatcatta aatcaaattt aacaaagagg atatttctct gtaagttctg
     5821 ctgccagttt tcttgcagtc tctacatttt cttcactctc gatcaccatt gcaatgatct
     5881 cggcgatctt atccatatcg tcctctttca ttccccgagt tgtaactgcc ggagttccga
     5941 gtcttactcc gcttgtcacg aacggagaac gaggatcgtt cggtattgta tttttattac
     6001 atgtaatatg tgcatcgtca agccgttttt ccagctcttt tccgcttacg tcatttccgg
     6061 taagatctac gagcataaga tgattgtctg tatctcctga aacgatcttc acgccgcgtt
     6121 ttttcaaacc ttcacagagc gcttttgcat tttttacaac ctgcatctga tattctttaa
     6181 attccggctc aagagcttct ttaaagcaga ctgcctttgc cgcgattaca tgcataagcg
     6241 gtcctccctg agttcccggg aaaattgctt tattaaaatt aaacttctcg ttcacttcat
     6301 tacttgaaag gatcattccg cctcttggac ctcttaatgt tttatgggtc gttgtcgttg
     6361 tcacatgtgc atacggaatc ggacttggat ggagacctgc tgccacaagt cctgcaatat
     6421 gagccatatc taccatgaga tacgagccga cttcatctgc gatttcacgg aaacgtttga
     6481 aatcgatcgt tcttgcataa gcgcttgcgc ccgcaatgat catcttcggt ttacattctt
     6541 ttgcaatttc caaaacctta tcataatcga tcactccgtc atcattgact ccgtaaggaa
     6601 cgacatgaaa atattttccc gacagattga ccggtgatcc atgagtcaaa tgtccgccgt
     6661 gatcaaggtt cattcccatg atcgtatcgc ccggtgtcag catcgcaaac tgaactgcca
     6721 tattagcctg cgctcctgaa tgaggctgta catttacata ctcacagcca aacagttttt
     6781 ttgcgcgttc tcttgccagt tcttcggcaa catccacaca ctgacagcct ccgtaatatc
     6841 tcttgcccgg atatccttct gcatatttat ttgtaagcgg acttcccatc gctgccatca
     6901 ctgctttgct cacccagttc tcagaagcga tcaattcaat atgggagttc tgtctcttca
     6961 tttccgctgt gatcacttcc gcaatctccg gatctgcatt tttgatttca tcgaagtcgt
     7021 acatcgtcat tatcctccat ctcatctgaa atttgaaatt tgaaatttca taaatttaca
     7081 ctttactatg acaaagcgtt agagttatta tagcatagcg gatgtatccc gtctacgtct
     7141 ttctaaagac agaaaatagg aaaggaagaa atattaacga tatgggagaa caaattttaa
     7201 ttgcagaaga tgacgcagat atcaacaatc tgcttgcaaa aatattacgc accgagcatt
     7261 ttaagccgat acaggcattt tccggaacag aagcaaaact gctctttatg cacaacacgc
     7321 cggacttaat cttactggat cttatgcttc cgggattatc cggcgaagaa cttctatccg
     7381 aacttcggga aaatctttct tctgacgtac cagtcatcat attgtctgca aaaattgccc
     7441 tggcagacaa agtgtcactt ttaaaaatgg gtgccgatga ttatattaca aagccgtttg
     7501 aacctgaaga agtcatagca agaatccagg ctgctttacg gcgtactgca acatcaaaaa
     7561 aactgtcttg cctaaattta gagttatatc cggattctag aaaagttact gttcacggaa
     7621 tcgaactttc tctgaccgcc cacgaatatg acttactcta tcttttcata aaaaatccag
     7681 agaaggtcta ttcccgcgaa atgctgtacg atctcgtctg gcacggaggc tattatgggg
     7741 aaaacaatac tgtca
//
LOCUS       DBMDXF010000084         7725 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2488_length_7725_cov_2.142970, whole genome shotgun sequence.
ACCESSION   DBMDXF010000084 DBMDXF010000000
VERSION     DBMDXF010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2488_length_7725_cov_2.142970"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="purD"
                     /locus_tag="ACZPD4_04730"
     CDS             complement(<1..1193)
                     /gene="purD"
                     /locus_tag="ACZPD4_04730"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1378411.1"
                     /translation="MKILIVGSGGREHAIAASVAKSPRADKIYCAPGNAGIAEYAECV
                     DIGAMEFEKLAEFAKEKEIDLCIVGMDDPLVGGLVDVLEDAGIRTFGPKKNAAILEGS
                     KAFSKDLMKKYDIPTAGYENFTDPKEAIAYLENAKFPIVLKADGLALGKGVLICQNFE
                     EAKEGVKTIMLDKKFGTAGNEMVIEEFMTGREVSVLSFVDGKTIKTMTSAQDHKRAGD
                     GDTGLNTGGMGTFSPSPFYTKEVEEFCEKYIYQRTVDAMAAEGRPFKGVIFFGLMLTE
                     EGPKVLEYNARFGDPEAQVVLPRMKNDILDVVEACVDGTLDQIDLQFEDNAAVCVVLA
                     SEGYPVQYEKGYPITGLEEFSKHEGYYCFHAGTKFEDGNIVTNGGRVLGVTAEGANLK
                     EARKNA"
     gene            complement(1288..1917)
                     /gene="purN"
                     /locus_tag="ACZPD4_04735"
     CDS             complement(1288..1917)
                     /gene="purN"
                     /locus_tag="ACZPD4_04735"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1378412.1"
                     /translation="MLKVVVLVSGGGTNLQAIIDGVKGGVIRNTKIVGVISNNKNAYA
                     LERARENHIPAKCISPKDYESRDVFNEKLLEAVNEYEPDLIVLAGFLVVIPPEMIAAY
                     RNRMINIHPSLIPAFCGKGYYGLKVHEAALARGVKVVGATVHFVDEGTDTGPIILQKA
                     VEVEQDDTPEMLQRRVMEQAEWKILPEAIHLIANGKVHVENGHAFIKNL"
     gene            complement(1911..2936)
                     /gene="purM"
                     /locus_tag="ACZPD4_04740"
     CDS             complement(1911..2936)
                     /gene="purM"
                     /locus_tag="ACZPD4_04740"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1378413.1"
                     /translation="MDYKKAGVDIEAGYRSVELMKEHVKKTMRPEVLGGLGGFSGAFS
                     LDKIKEMEEPVLLSGTDGCGTKVKLSMVLDKHDTIGIDAVAMCVNDIACAGGEPLFFL
                     DYIACGKNEPEKIASIVSGVAEGCLQSGAALIGGETAEHPGLMAEDEYDLAGFAVGVC
                     DKKDMITGENLSAGDVLIGMASTGVHSNGFSLVRKVFDITKESLDTYYDELGCTLGEA
                     LLAPTRIYVKALKSIKEAGITVKACSHITGGGFYENVPRMLIDGTRAVIEKDSYPIPP
                     IFKMLAKEGDIEEQMMYNTFNMGLGMIVAVDPADVEKTLEAIKAAGDAPYVVGRIEAG
                     EKGVDLC"
     gene            complement(2950..3456)
                     /gene="purE"
                     /locus_tag="ACZPD4_04745"
     CDS             complement(2950..3456)
                     /gene="purE"
                     /locus_tag="ACZPD4_04745"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1378414.1"
                     /translation="MPKVGIVMGSDSDLKVMSKAASMLEKFGIDYEMTIISAHRMPDV
                     FFEWAKAAEGKGIKVIIAGAGMAAHLPGMCAALFPMPVIGVPMSGKNLDGMDALYSIV
                     QMPPGIPVATVAIDGGMNAAILAAKILAVSDEEVLKKLKDYSAEMKDTVQAKADRLAE
                     VGYEEYLK"
     gene            complement(3482..5575)
                     /locus_tag="ACZPD4_04750"
     CDS             complement(3482..5575)
                     /locus_tag="ACZPD4_04750"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1378415.1"
                     /translation="MQEVSEKYNNDSYRERHALSVERLRTLPFEESVEDSYIAYFRLV
                     ALFLLEVENVRIQVESGKWDQYNEEDMRQINEILYSDIVGDAYDKSYANPKYACSWFE
                     PEMGRLLSFLYVEMRSGIPYAFEGRLDYLTILYELFIEVYTYFENCRADGAEPEIRRV
                     RDIVYWYASDYCDVFLADRIKEQIDPSYSFAADIIENADLSSDRYLYRFGEYITENEL
                     GTARRLRSLPEETIQKMADVYTEGYRIGFINTGKDLSKKSVVNIRYSLGFERVIRAAI
                     ANFRAMGLKPVIYRAASGVITKREHHKIGYFGAVANWQYEYDHRQDQALFMDKRYIER
                     RLEVMHTVYEQNKEQAAQFAGPAVMETFGEKPFSPKAVPEAPAYCEEQRELALQYDSR
                     SGQITNEYIKGEERSFTIIAYPVPEIGPKYEEIFDEVIRINTLDAKLYEKVQQTMIDA
                     LDQGEKVRVIGKGENRTDMEIRLWSLKDARKETIFENCVADVNIPVGEVFTSPVLEGT
                     NGVLHVSRVYLDGLQYKDLELKFKDGKIVDYRCGNFKDEEEGRRYIFDNVLKNHDTLP
                     LGEFAIGTNTTAYAAGKKYGIEDKMPILIAEKTGPHFAVGDTCYSWSEDVRVYNPNGK
                     EIVAKDNSCSLKRKEDPSAAYFQCHTDITVPYEELEEISVVTKDGKHIILIKDGRFVL
                     PGTEILNEPLKQLEN"
     gene            complement(5652..6038)
                     /locus_tag="ACZPD4_04755"
     CDS             complement(5652..6038)
                     /locus_tag="ACZPD4_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6142 family protein"
                     /protein_id="HIR1378416.1"
                     /translation="MFGKEKKAEDKKKETALKREENRKKSGVKHKKPKQKQAGNGNLS
                     CLFGLGSAAVLAGCIAYAFVRRGEAPGIIGGITIISLILAGYGIYSAIIGFRERERSY
                     LTCKIGIVMNAAAIISFFAVFIRGLN"
     gene            complement(6007..6489)
                     /locus_tag="ACZPD4_04760"
     CDS             complement(6007..6489)
                     /locus_tag="ACZPD4_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1378417.1"
                     /translation="MSIKKERIYRIFGKILFLLYIVFLIYFLFLAEWYGRTEVTEEYR
                     YNLELFKEIRRFLTYREQLGMFTVLANLLGNIMIFVPYGFFISMAGKSMGFFKILFFS
                     MALSLGVETIQLFTRVGSFDVDDILLNTIGGVLGYILFVVYSRIRRKRYVRKGKKGRR
                     "
     gene            complement(6736..6885)
                     /locus_tag="ACZPD4_04765"
     CDS             complement(6736..6885)
                     /locus_tag="ACZPD4_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378418.1"
                     /translation="MEKAYLTMKNSGASSITLGIIVLVVGVATGILAIVNGAKLLKHK
                     KEITF"
     assembly_gap    6954..6963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6964..7615)
                     /gene="pyrE"
                     /locus_tag="ACZPD4_04770"
     CDS             complement(<6964..7615)
                     /gene="pyrE"
                     /locus_tag="ACZPD4_04770"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1378419.1"
                     /translation="MESYKQEFIEFMVDSDVLKFGEFTLKSGRKSPFFMNAGGYVTGS
                     QLKKLGEYYAKAIHDTYGDDFDVLFGPAYKGIPLAVVTAIAYSELYGKEVRYCSDRKE
                     EKDHGADKGSFLGSKFKDGDRVIMIEDVTTSGKSMEETVPKVKQAADVEIVGLIVSLN
                     RMEVGKGGEKCALDEVKDLYGFDTAAIVTMEEVVEHLYNKPYNGKVVIDDTLKQAID"
BASE COUNT         1895 a         2119 c         1263 g         2438 t
ORIGIN      
        1 gcattttttc ttgcttcttt cagattagcg ccttctgcag ttacaccaag cacacggcct
       61 ccgtttgtca cgatgtttcc gtcctcaaat tttgttcctg catggaaaca gtaataccct
      121 tcatgtttac tgaattcttc aagacctgtg ataggatagc ctttttcgta ctgaaccgga
      181 tagccttcag atgcaagcac aacgcacacg gccgcattat cttcaaactg aagatcgatc
      241 tgatccagag ttccgtccac acatgcttca acaacgtcca aaatatcatt tttcatacgc
      301 ggaagcacaa cctgagcctc cggatctccg aagcgggcgt tgtactccaa cactttcggt
      361 ccttcttctg taagcatcag accgaagaaa ataacgcctt taaacgggcg gccctctgca
      421 gccatagcat ctaccgttct ctggtaaata tatttttcac aaaattcttc tacttctttt
      481 gtatagaacg gacttggtga aaatgttccc attccgcctg tattcagccc tgtatcaccg
      541 tcaccggcac gtttgtgatc ctgcgcgctt gtcatcgttt tgatcgtctt tccgtctaca
      601 aatgaaagaa cagatacttc gcgacctgtc atgaactctt caatgaccat ctcgtttccg
      661 gctgtgccga attttttgtc aagcatgatc gtctttacgc cttcttttgc ctcttcaaaa
      721 ttctgacaga tcagcactcc tttaccaagt gcaagtccgt ctgccttcaa tacgataggg
      781 aactttgcat tctcaagata ggcgattgct tcctttggat ctgtaaaatt ctcatatcct
      841 gccgtaggaa tatcgtattt tttcataaga tcttttgaaa acgcttttga tccttccaaa
      901 atagcggcgt ttttctttgg accgaacgta cggattcccg catcttccaa tacgtccaca
      961 agacctccga caagcggatc atccattccg acgatgcaaa gatcgatctc tttttctttg
     1021 gcaaattctg ccagtttttc aaattccatt gctccgatgt ccacgcactc tgcgtattct
     1081 gcaattcccg cgtttccggg cgcgcagtat atcttatctg ctctgggact ttttgccaca
     1141 cttgccgcaa tggcatgttc tcttccgccg cttccgacga tcaatatttt catgatagat
     1201 tcctcctgtc tgtacaaacc gttttacgtt ccgaaatgac cgcccggttt tttcgtcccg
     1261 gcggtcattt tttaatgttt cgacattcta taaatttttt ataaaagcat gtccgttttc
     1321 aacatgcact tttccgttcg cgatcaaatg gatcgcttcc ggaaggattt tccactctgc
     1381 ctgctccatc actcttcttt gaagcatctc cggtgtgtca tcctgctcaa cttctactgc
     1441 tttctgcagg atgatcggcc ctgtatcggt tccttcatct acaaaatgaa cggtcgcgcc
     1501 gactacttta acacctcttg caagcgctgc ttcatgcact tttaatccat aatatccttt
     1561 cccgcaaaac gccggaatca acgacgggtg aatattaatc atccggtttc tgtaagctgc
     1621 gatcatttcc ggagggataa caacaagaaa tcctgccaaa acgatcagat ccggttcgta
     1681 ctcgttgacg gcttccaaaa gtttttcatt aaacacatcc ctggattcat aatctttcgg
     1741 cgagatacat tttgccggga tgtgattctc tctcgcccgc tcaagcgcat atgcattctt
     1801 gttattgctg ataactccga cgatctttgt attcctgatg acgccgcctt ttacgccgtc
     1861 aatgatcgcc tgcagattcg tacctcctcc ggatacgagc acaacgacct ttagcataag
     1921 tctactcctt tttctcccgc ttcgatgcgg ccgactacat acggcgcatc tcctgccgct
     1981 ttgatcgctt ccagagtttt ctccacatct gccggatcaa cggcaacgat cattccaagt
     2041 cccatgttaa acgtattgta catcatctgc tcttcgatat cgccttcttt tgcaagcatc
     2101 ttgaagatcg gcgggatcgg ataactgtct ttttcgatca ccgctcgagt accgtcgatc
     2161 agcatacgcg gaacattctc atagaaaccg ccgcctgtaa tatggctgca tgctttcacc
     2221 gttattcccg cttctttaat actcttaagc gctttcacat aaattcttgt cggagcgaga
     2281 agcgcctctc cgagagtaca tcccaattca tcgtaatatg tatcaagaga ttcttttgta
     2341 atatcaaata cttttcttac aagtgaaaat ccgttgctgt gaactcccgt agacgccatt
     2401 ccgatcaaaa cgtctccggc agacagattt tctcctgtga tcatatcttt tttatcgcat
     2461 actcctaccg caaatccggc aagatcgtac tcgtcttctg ccataagtcc cggatgttcc
     2521 gccgtttcgc ctccgatcag cgccgctccc gactgaagac atccttctgc cactccgctt
     2581 acgattgatg cgatcttctc aggctcattc ttgccgcatg caatataatc aaggaaaaac
     2641 agtggttctc ctcccgcaca ggcaatatca ttcacacaca ttgccacagc atcgattccg
     2701 atcgtgtcat gcttatcaag caccatcgac agcttcactt tcgttccgca gccgtctgtt
     2761 cccgacagca gtaccggctc ttccatttct ttgatcttat caagagaaaa tgctcccgaa
     2821 aatcctccga gtcctccgag cacttccgga cgcatcgtct ttttcacatg ctctttcata
     2881 agttctactg atctgtaacc ggcctctatg tcaactcctg ctttcttata atccatacca
     2941 ctttctcctt tactttaaat actcctcata tccgacctcg gcaagacgat cggctttcgc
     3001 ctgaacggta tctttcatct ctgccgaata gtcttttaac ttttttaata cctcttcatc
     3061 tgatacagcg aggatttttg ccgcaagaat cgcagcgttc attccaccgt caatcgcaac
     3121 agtcgcaacc ggaattcccg gcggcatctg aacgatcgag taaagagcgt ccataccatc
     3181 cagattcttt cctgacatcg gcactccgat caccggcatc gggaacagcg ccgcacacat
     3241 tcccggaaga tgtgcagcca ttccggctcc tgcgatgatt actttaatcc cttttccttc
     3301 cgcagctttt gcccactcaa aaaatacatc cggcatacga tgtgcggaaa tgatcgtcat
     3361 ttcataatca attccgaatt tttccaacat tgacgcagct ttgctcatca ctttcaggtc
     3421 agagtcactg cccattacga ttcctacttt tggcacgtta tttcctcctt aaaatctcaa
     3481 atcaattttc gagttgtttt aatggttcat ttaaaatctc agttcccgga agcacaaatc
     3541 ttccgtcttt aattagtata atgtgtttcc cgtcttttgt cactacactt atttcttcta
     3601 actcttcata aggtacggtt atatctgtat gacattgaaa gtatgccgca gacggatctt
     3661 cttttctttt cagcgaacaa gaattatcct ttgcaacgat ttcttttccg ttcggattat
     3721 atacacggac gtcttcgctc catgaatagc atgtgtcgcc cactgcaaaa tgaggtcctg
     3781 ttttctctgc gatcaaaatc ggcatcttat cttcgatccc gtattttttt cccgcggcat
     3841 aagcagtcgt atttgttccg atcgcgaatt cgccaagcgg aagagtatca tgatttttca
     3901 aaacattatc aaaaatatat cgtctgcctt cttcctcgtc tttaaaattg ccgcatctat
     3961 aatcaacgat ctttccgtct ttaaatttca attcaaggtc tttatactgc aaaccgtcaa
     4021 gatacacccg gctgacatgc aaaactccgt tcgttccttc cagcaccggt gatgtaaaca
     4081 cctcgcctac cggaatattt acatctgcca cacagttttc aaaaatcgtc tctttccgag
     4141 catcttttaa cgaccaaagc cgaatctcca tatccgtccg gttctctccc tttccgatca
     4201 cacggacttt ttctccctga tcgagcgcat cgatcatcgt ctgttgaact ttctcatata
     4261 attttgcatc taacgtatta attcgtatta cttcatcgaa aatttcttca tatttcggac
     4321 cgatctccgg cacaggatat gcaatgatcg taaaacttct ctcttctcct ttgatatatt
     4381 catttgtgat ctgaccggat ctgctgtcgt attgaagcgc aagctccctt tgctcttcac
     4441 agtaagccgg agcttccggt actgctttcg gtgaaaacgg cttttctccg aatgtttcca
     4501 tcacggcagg tcctgcgaac tgcgctgcct gttctttatt ttgttcataa acagtatgca
     4561 tcacttccag acgccgttca atatagcgtt tatccataaa taacgcctga tcttgtctgt
     4621 gatcgtactc atactgccag tttgccacag cgccgaaata tccgattttg tgatgctctc
     4681 ttttcgtgat cacacccgac gccgccctgt atattacggg ttttaacccc atagcgcgaa
     4741 aatttgcgat cgccgctctt atgacccgtt caaatccgag gctgtaacga atatttacga
     4801 ctgatttttt ggacagatct tttcctgtat tgataaatcc gatccgatat ccttccgtgt
     4861 aaacgtccgc catcttttga atcgtctctt cgggaagcga cctgagacgg cgcgcagttc
     4921 ccagctcatt ttctgtaata tattctccga aacgatacaa atacctgtcg ctgctcaaat
     4981 ctgcattttc gataatatct gccgcaaacg aatacgacgg atcgatctgt tctttaatcc
     5041 gatctgccaa aaatacgtcg caataatcgc ttgcatacca atacacgata tctcttactc
     5101 ttcgtatttc cggttcagcg ccatctgccc ggcaattttc aaaatacgta tatacctcga
     5161 taaacaattc atacaaaatc gtcagataat caagtctgcc ttcaaacgca taagggatgc
     5221 cggagcgcat ttccacatag agaaaactca aaagccttcc catctccggc tcaaaccagc
     5281 tgcatgcata tttcggattt gcatagcttt tgtcgtaagc atccccgaca atatcactat
     5341 aaaggatttc gttgatctgt ctcatgtctt cttcattata ttgatcccat tttccgcttt
     5401 ccacctgaat ccgcacattt tccacttcaa gcaaaaacag ggcaaccaat ctgaaatacg
     5461 cgatataaga atcttccaca gattcctcaa aaggcagcgt gcgaagacgt tctaccgaca
     5521 acgcatgccg ttccctatat gaatcgttgt tgtatttttc tgacacttcc tgcattttcc
     5581 tttccctccc gctcatctgt ttacttttct ttattattta tcttttttca ctacttttta
     5641 tcttttgctt actaattcaa acctctgata aataccgcaa aaaatgaaat aatcgccgcc
     5701 gcattcatga caataccgat cttacacgtc agataactgc gttctctctc ccgaaatcct
     5761 ataattgccg aataaattcc gtatcctgcc agtatcaaag aaataatcgt aatacctcct
     5821 atgatcccgg gggcttctcc tcttcggaca aacgcgtatg ctatacatcc cgccaacact
     5881 gcggccgaac ctaatccgaa cagacacgaa agatttccgt tgcccgcctg tttttgcttt
     5941 ggctttttat gtttcacccc gctttttttc cggttttcct cccgtttcaa agcagtttct
     6001 tttttcttat cttctgcctt tttttccttt ccgaacataa cgttttctcc ttatccgact
     6061 gtataccaca aacaaaatat atcctaaaac tccgccgatt gtatttaata aaatatcatc
     6121 gacatcaaaa cttcctactc tcgtaaaaag ttgtattgtt tccacgccca gactcagcgc
     6181 catactgaaa aataatattt taaaaaatcc catggacttt cccgccatgg aaataaagaa
     6241 accataagga acaaagatca taatattccc gagaaggttc gcaagcaccg taaacatgcc
     6301 aagctgttct ctataagtaa gaaaacgtct gatttcttta aataattcaa gattatatct
     6361 atactcttcc gtcacttccg ttcttccgta ccattccgca agaaacagaa aataaatcaa
     6421 aaaaacaata tatagtaaaa acaggatttt cccaaatatc ctataaatcc gctctttttt
     6481 tatactcaaa aataactctc ctcacacaag gaactctccc tatcagtaat tttttcaaat
     6541 caccggaagc gctaaaaaag cgcgctccgg gactatacac ggccgcaaag tctcaggcaa
     6601 acccagactt cgttccgccg ccgttgcaaa aagggactgc ctgtattccg gcttagtccc
     6661 ttatttttct ttctgtttat ttatctctct ttcatccgtg tcctttcaca gtttccctga
     6721 aagctgtcaa gctgcttaaa atgtaatctc ttttttatgt ttgagtaatt ttgctccgtt
     6781 cacgattgca agtattcctg ttgccactcc gacaacgaga acgataatcc caagtgtaat
     6841 gctgcttgca ccactgtttt tcattgtaag atatgccttt tccataaagc gcctccccgt
     6901 tttattttac tccatatgtt tcatagtatg tatcgatcgc agcctttaat gtannnnnnn
     6961 nnncgtcgat cgcctgtttc aatgtatcgt cgatcacaac ttttccgtta tacggcttgt
     7021 tgtacaagtg ttccacgact tcttccatcg tcacgatcgc ggccgtatcg aaaccataca
     7081 gatctttcac ctcgtcaagg gcgcattttt ctcccccttt tccgacctcc atacgattaa
     7141 gagatacgat taatccgacg atctccacat cagcggcctg ctttactttc ggaacggtct
     7201 cttccatcga ctttcctgac gtcgttacat cttcgatcat gataactcga tcgccgtctt
     7261 taaatttact tccgaggaag cttcctttgt ctgctccgtg atctttctct tcttttctgt
     7321 ccgagcagta tcgtacttct tttccataca actcactgta agcgatcgca gttacaaccg
     7381 caagaggaat ccccttataa gcaggtccga acaacacatc aaaatcatct ccgtatgtgt
     7441 catggatcgc tttcgcataa tattcgccga gctttttaag ctgcgaacct gtcacatatc
     7501 cgcctgcatt cataaaaaac ggagatttcc ttccgctttt caatgtgaat tctccaaatt
     7561 tcagcacatc gctgtccacc ataaactcaa taaattcctg cttataactt tccatctttc
     7621 ttaaaacctc cgtatttacg ggcttttctc gcccttactt tctatgtttc atcccctgct
     7681 acaccatttt tacaccactt catgctcaac tacaccttct cttaa
//
LOCUS       DBMDXF010000085         7677 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2505_length_7677_cov_4.401135, whole genome shotgun sequence.
ACCESSION   DBMDXF010000085 DBMDXF010000000
VERSION     DBMDXF010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_7677_cov_4.401135"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            207..2582
                     /locus_tag="ACZPD4_04775"
     CDS             207..2582
                     /locus_tag="ACZPD4_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1378420.1"
                     /translation="MNNKTLIKLEFDKIISMLENEASSFRGKQLCRRLKPVTDLTKID
                     LLQEQTAAAFTRIIKKGRISFGDAAPVEESLKRLEIGGALNTAELLRICRLLSNTARA
                     KSYGRHDTQEDLADCLDIYFDGLEPLTPLSNEIERCIISEDEISDDASSALKHIRRSI
                     NNLNDRVHTTLSGLVNGSLRTYLQDALITMRGDRYCIPVKAEYRSQVQGLIHDQSASG
                     STLFIEPMAIVKLNNDLKELYVQEQDEIRKILASLSEETAQYIEEIRTDYRSLTDLDF
                     IFARGALALTMRASRPILNEEGRIRIREGRHPLLDQKKVVPITVSLGDEFSLLIITGP
                     NTGGKTVSLKTVGLLTLMGQAGLHIPAGDRSEIAVFRQVYADIGDEQSIEQSLSTFSS
                     HMTNIVSFLKKVDDRSLVLFDELGAGTDPTEGAALAIAILSHLHKRNIRTMATTHYSE
                     LKIYALSTPGVENACCEFDVESLRPTYRLLIGIPGKSNAFAISGKLGLPGYIIDDAKK
                     RLSEQDVSFEDLLSDLEASRRTIEKEQAEIAAYKKEAETLKRQAVQKQEKLEEQRDRI
                     IREANEKANAILREAKEVADETIRNFHKFGKENISAAEMEKERERLRKKIKDTSASAS
                     LKTNKPKKTYKPSDFKLGESVKVLSMNLTGTIGSLPDARGNVTVQMGILRSQVNISDL
                     EIIEEVSPYAPKRMNRTAKSKIKMSKSLSVSPEINLLGKTVDEAVAELDKYLDDALLS
                     HLNSVRVVHGKGTGALRKGIHEYLRRQKHVKSYRLAEFGEGDAGVTIVELG"
     gene            2612..3313
                     /locus_tag="ACZPD4_04780"
     CDS             2612..3313
                     /locus_tag="ACZPD4_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1378421.1"
                     /translation="MIGKQRILIVDDDENIAELISLYLTKECFDTKIVFNGEDALSTF
                     ETYEPNLILLDLMLPGIDGYQVCREIRAKSGVPIIMLSAKGEIFDKVLGLELGADDYI
                     LKPFDSKEMIARVRAVLRRYHPIKNEPSQAEKPKCVEYEGLTINLTNYSVLCDGNTVE
                     MPPKELELLYCLASSPNQVFTREQLLDRIWGYEYLGDTRTVDVHIKRLREKLKDHPHW
                     KLGTVWGIGYKFETK"
     gene            3338..4744
                     /locus_tag="ACZPD4_04785"
     CDS             3338..4744
                     /locus_tag="ACZPD4_04785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1378422.1"
                     /translation="MRSTLHIKFIALYIIFGFLCLFSTATLTEQLVTDKLTEDTSHTL
                     YREATFVANDYLPSYFSGDSSIYNVQSQLSAMRLYFNASIWFVDSTGRTLTSSSLEDT
                     VCPDRIKDFNPAEIGGNRYITGTYHGYFDEDMITVMAPVTQGFSTKGYLLIHSPVSAI
                     EERCAPIMTQVYISTAVIFALSLIFLLGVQFFIHRPLRQISEAAKQYAIGNLDYEIPV
                     HTHDELGYLSASLNYMAVRLKDMDDYQKKIVANVSHDFRSPLTSIKGYVEAMADGTIP
                     PELYGKYLNIILFETERLTDLTRDLLTLNEFDTKELLLNKSDFDIQEVLKSTAASFES
                     ACTQKHISIELLLMPDAVFVLADKRKIQQVLYNLIDNAIKFSENDSLITVEVSEKNEK
                     VFISVKDHGIGIPRQEQTKIWERFYKSDLSRGKDKKGTGLGLSIVKEIIQAHDEHINV
                     VSTEGVGTEFIFSLSRSG"
     gene            complement(4832..6085)
                     /locus_tag="ACZPD4_04790"
     CDS             complement(4832..6085)
                     /locus_tag="ACZPD4_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1378423.1"
                     /translation="MKRKITAAAAFALCVAMGVCACSPSEKEDTFTGKEKEELAWQPN
                     LDRISPEVYADISNLDLKPGTYISVIGKREGTAYWSEVQAGVEQAAEDINKHLGYKGE
                     DKIKVLYNAPADSENIDEQVNILDEELARYPDVIAVASVAEDASAVQFDLAAENGIAV
                     VAFDSRNNYQGIQCTCMTDNVAAAKEGARKMSEAIEEKGEILLIAQDSVSGTAKERTK
                     AVTEEITANYPNVKVVETIYMDQFDDLKMQAAADELGVTKEQLAEWTVESSGQTPADE
                     GEEAMSEEVRTKLEDIRAVADGMTDADVVARYMEKYPNLKGCFAVNADAVLLAMDAFE
                     TAENEEDIVLMGFDAGKEQIAALKNGTIDGLIVQNPFGMGYATVVAAARAALEIGNEA
                     TVDTGYVWVDKENMDDPDVKGMLYE"
     gene            complement(6254..7603)
                     /locus_tag="ACZPD4_04795"
     CDS             complement(6254..7603)
                     /locus_tag="ACZPD4_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_337493284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HIR1378424.1"
                     /translation="MKEGKITLSQEQLKTLTVINRFIDKSISRQQAAELLGLSTRQIT
                     RLKKGVLTSGAQSLIHKNTGRKPVHSVSDELKEAVLKIYSRPEFSNANFLHFKDILSA
                     DFGIQLSYSALSSILKNAGFESPKKKKIRHRTHRRKRKPHPGQLIQIDATPYEWFGGN
                     IKYALHGAIDDATGQVVGLFLTQNECLYGYLETMRQCCSDFGIPQTVYSDNHTIFRSP
                     KTGKLTVDELIAGKTVHLTQFGRSLHELGIDLIFAKTPQAKGRIERLWVTLQSRLPVE
                     FAKRGITTVSEANRFLETEYRELFNQRFAVEPEAESIFVSAAKDLNLDSILCVKHTRK
                     TDAAGTFSFKNRCFQILDEGFPIINARREITVLINPRFGIRVEYGGRIYNTIRYLKPQ
                     NKNANTEVPQKVSKTVEPHLQLRHSSDEWKAIWWMEDYNLSLKFLYELFFEKQQSAS"
BASE COUNT         2185 a         1829 c         1586 g         2077 t
ORIGIN      
        1 agtatttccg gattatgggc ggttgggatg tcaccgcttc cccgggtgtg actgcacgcg
       61 aaggcatgga cttattttcc tccaaattct agtatacctg attcaatcct ctatgaaaag
      121 gtttttctta cttcctgaaa gtccttttct ttatacggat aatagagtag aatgaggata
      181 caaacattac agacaggtga agatttatga ataacaaaac attaataaaa cttgaatttg
      241 ataaaattat ttccatgttg gaaaatgagg caagttcttt ccgcggaaaa caactttgcc
      301 gacggctgaa accggtaacg gatcttacga agatcgattt gcttcaggaa cagaccgccg
      361 ccgcttttac aaggatcatt aaaaaggggc ggatttcttt cggcgacgca gctcctgtgg
      421 aagaatctct caaacgactt gagatcggag gcgctctaaa tacagcggaa cttctccgca
      481 tctgccgtct tctttcaaac actgcgcggg ccaagtcata cggacggcat gacacgcagg
      541 aagatcttgc cgactgtctt gatatttatt ttgacggact tgagccgctc acacctcttt
      601 caaatgaaat tgaaagatgc atcatttccg aagatgaaat cagtgatgac gcaagcagtg
      661 cgctaaagca tatccgaagg agcataaata atctcaacga ccgtgttcac acaacacttt
      721 caggtcttgt aaacggttct ttaagaacgt acctgcagga tgctctgatc acaatgcgcg
      781 gcgaccgcta ttgtatccct gtcaaagccg aataccgcag tcaggtacaa gggctgatcc
      841 atgaccagtc cgcatccggt tctacattat ttattgaacc tatggctatt gtaaaactga
      901 acaatgactt aaaagaatta tatgtacaag aacaggatga gatccgaaag attcttgctt
      961 ctttaagcga agagaccgca caatatatcg aagagatccg aacagactac cgatctctca
     1021 ccgatctgga tttcattttt gcaagaggcg ctcttgctct tactatgcgc gcaagtcgtc
     1081 cgattctaaa tgaagaagga cgtatccgta tccgggaagg acgccaccct cttctcgacc
     1141 agaagaaagt tgtcccgatc accgtatctc tcggcgatga attttctctt ctcatcatca
     1201 ccggacctaa tacaggcgga aagaccgtct ctttaaaaac ggtcggactt ctcaccctca
     1261 tggggcaggc cggacttcat attccggcag gcgaccgttc ggaaatcgcc gtattccgac
     1321 aggtttacgc cgatatcgga gatgagcaga gcatagaaca aagtctgagt acattttctt
     1381 cacatatgac aaatattgtt tcctttttga aaaaggtcga cgatcgttct ctcgttcttt
     1441 tcgacgaatt aggcgcagga accgacccga cagaaggagc ggcccttgcc attgctattt
     1501 tatcgcatct ccataagcga aacatacgca ctatggcaac aacccattac agcgagctga
     1561 aaatctatgc cctttctacc ccgggcgttg aaaatgcctg ctgtgaattc gatgtggaaa
     1621 gtctccggcc gacttaccgg cttctcatcg gtattccggg taaaagtaat gcttttgcga
     1681 tttccggaaa gctcggactt cccggctata ttatcgacga tgcaaaaaaa cgtctgagtg
     1741 aacaagacgt ctcttttgaa gatctcctaa gcgatcttga ggcaagcaga cgcacgatcg
     1801 aaaaagaaca ggctgaaatt gccgcttata aaaaagaggc cgaaacttta aaacggcagg
     1861 ctgtgcaaaa acaggaaaag ctcgaagaac aacgtgaccg catcatccgt gaagcaaatg
     1921 aaaaagcaaa tgcaatacta agagaagcga aagaagttgc cgacgagacg atccgcaatt
     1981 ttcacaaatt cggaaaggag aatatctccg ccgctgaaat ggaaaaagaa cgggagcgtc
     2041 tgcgcaaaaa gatcaaagat acttccgctt ctgcttcact gaaaacaaac aaaccgaaaa
     2101 agacttacaa accgtctgat ttcaaactcg gagaatctgt aaaagtatta agtatgaatc
     2161 tgaccggcac catcggctct ctccccgatg caagaggcaa cgtcacagtt cagatgggaa
     2221 tccttcggtc acaagtcaat atttccgatt tggaaattat cgaagaagta tcgccttacg
     2281 caccgaagcg gatgaaccgt acggcaaaaa gcaagatcaa gatgagcaag tcactttctg
     2341 taagtcctga gatcaatctg ctcggaaaaa cagtagacga agcggtcgcc gaactggata
     2401 aatatttaga tgacgccctg ctctcgcatc taaacagcgt acgcgtagtt cacggaaaag
     2461 gaaccggagc tttaagaaaa ggaatccacg aatatctcag aagacaaaaa catgtaaaat
     2521 cataccgcct tgccgagttc ggagaaggcg atgccggagt cacaatcgtt gagctcggat
     2581 aacgggttca cccataaaac ggaggaaaaa tatgatcgga aaacaaagaa tactaatcgt
     2641 tgacgatgat gaaaatatcg cagaacttat ctctctctat cttacaaaag agtgttttga
     2701 cacaaaaata gtatttaacg gagaagacgc cttgagcaca tttgaaacat acgaaccgaa
     2761 tctgattctt cttgacctta tgcttccggg catcgacggc tatcaagtat gccgggagat
     2821 cagggcaaaa tcaggcgtgc cgattattat gctttcggcg aaaggggaaa tattcgacaa
     2881 agtactcggt ctggaactcg gtgcggacga ttatatctta aagccttttg attcaaaaga
     2941 aatgattgcg cgtgtgcgcg ccgttcttcg ccgttatcat ccgataaaaa acgaaccgtc
     3001 ccaggcggaa aagccaaagt gtgtcgagta tgaaggactg accatcaacc tgacaaatta
     3061 ttctgttctc tgtgacggaa acactgttga aatgccgcca aaagaactgg agcttttata
     3121 ctgtctcgcc tcatctccga atcaggtatt tacaagggag cagctccttg accgtatatg
     3181 gggttatgaa tatctcggtg acacccgcac tgtcgacgtc catatcaaac gtctccggga
     3241 aaaacttaaa gatcatcctc attggaaatt aggaaccgta tggggcatcg gctataaatt
     3301 tgagacaaaa taacagataa acaggagact accgcgtatg agaagcactc tgcacattaa
     3361 atttattgca ctttatatca tatttggatt tttatgtcta ttctcaactg caacgcttac
     3421 agagcagctt gtgacagata agcttaccga agacacttct catacgcttt acagagaagc
     3481 tacttttgtt gccaatgatt atctcccatc ttacttctcc ggcgattctt ccatttataa
     3541 cgtccagtca cagttgtctg caatgagact gtacttcaac gcttctatct ggttcgtaga
     3601 cagtaccgga agaacactta cctcttccag tttagaagac actgtatgtc ctgataggat
     3661 caaagacttc aaccctgccg aaatcggcgg aaaccgctat attacaggta catatcacgg
     3721 atattttgac gaagatatga tcaccgttat ggcgcctgtg acacaaggtt tttctacaaa
     3781 gggatacctt ctgatccaca gtcccgtctc tgccatcgaa gagagatgcg ctccgatcat
     3841 gactcaggta tacatctcca cagcagtcat tttcgcactt tctcttatct ttctcctcgg
     3901 cgtgcagttt ttcatccatc gtccgctcag gcagatttcc gaagcggcaa aacaatacgc
     3961 tatcggaaat cttgactatg agattccggt acacactcat gatgaactcg gatatctctc
     4021 tgcttcactg aattacatgg cagtcagact gaaagatatg gatgactatc aaaaaaaaat
     4081 tgtcgcaaat gtctctcatg attttcgttc tcctctcacg tccataaaag gatatgttga
     4141 ggcaatggcc gacggaacga tcccgcctga attgtacgga aaatatttaa atattatttt
     4201 gtttgaaaca gagcgtctca ccgacctcac gcgagatctg ctgacattaa atgaattcga
     4261 tacaaaagaa cttcttctta acaagtcaga ttttgacata caggaagttc tgaaaagtac
     4321 cgccgcatct tttgaatctg cctgtaccca aaaacatatt tccatcgaac tgcttctcat
     4381 gcctgatgct gtttttgttt tggcagacaa acgtaagatc cagcaagtcc tctataactt
     4441 gatcgataat gcaattaaat tcagcgaaaa cgattctttg attactgtag aggttagtga
     4501 aaagaacgaa aaagtattta tttccgtaaa agatcacgga atcggaatcc cgcggcaaga
     4561 gcagactaaa atatgggagc gtttttacaa atcagacctt tcccgcggca aagataaaaa
     4621 agggacaggc cttggcctgt ccatcgtaaa agaaattatt caagctcatg acgagcatat
     4681 taatgtagta agtaccgaag gagtcggcac tgaatttatc ttttcactca gtcggagcgg
     4741 gtaactcttt acccgctaat ggaaaacaga atttcctcgc ttacgcctga cgaaaactat
     4801 ttgtaaagca tccgcttacg cctgacgaag actattcgta aagcatcccc ttcacgtccg
     4861 ggtcgtccat attttcttta tccacccaaa catatcctgt atcgacggtt gcctcatttc
     4921 cgatctcaag cgcagcccgc gccgccgcca caactgtcgc atatcccatg ccgaacggat
     4981 tttgcacaat aagaccatct atcgttccgt ttttcaatgc cgcgatctgt tcctttccgg
     5041 catcaaatcc catcaacacg atatcttctt cattttctgc tgtctcaaaa gcatccattg
     5101 ccagtaaaac cgcatcggca tttaccgcaa aacagccctt caggttcggg tatttttcca
     5161 tataacgggc aaccacatcc gcatctgtca tcccgtccgc gacagcgcga atatcttcca
     5221 gtttcgtgcg cacctcttcc gacatcgctt cttccccttc atctgccgga gtctgaccgg
     5281 acgattccac cgtccactcg gcaagctgct ctttcgtcac cccgagttcg tccgctgcag
     5341 cctgcatctt aagatcgtca aactgatcca tatagatcgt ctctacaacc ttcacattcg
     5401 gataatttgc cgtaatttct tctgtcactg cctttgttcg ctcttttgcc gttcccgaaa
     5461 cagaatcctg tgcgatcaac agaatctctc ctttttcttc gatcgcctcg ctcattttcc
     5521 gagccccttc ttttgccgcc gccacattat ccgtcatgca ggtgcactga attccctgat
     5581 aattgttcct cgaatcaaat gccacgactg caattccgtt ttctgccgca agatcgaact
     5641 gaacagccga cgcatcttcc gctacgcttg caacagcgat cacatccgga tatcgcgcca
     5701 gctcctcgtc aaggatattc acctgttcgt caatattctc actgtctgcc ggagcgttat
     5761 aaagaacttt tatcttgtcc tcccctttat atcccagatg cttattaatg tcttccgccg
     5821 cctgctcaac tccggcctgc acttcgctcc agtaagccgt tccctctctc tttccgatga
     5881 ctgatatata cgtccccggc ttcaggtcaa gattactgat atctgcatat acttcagggg
     5941 aaatccggtc aagattcggc tgccacgcaa gctcttcctt ttcctttccc gtaaatgtat
     6001 cttccttttc agacggactg catgcgcaga ctcccattgc cacacacaat gcaaatgccg
     6061 ccgctgctgt aatttttctc ttcattataa tttatccctc cgcttttatc cttttataat
     6121 acaccatatc atacaaaata aatgtgaatt tttactaaac ttttatttct gtcaataact
     6181 tatgaaaatg tcacttttta atatagaaat ttatttgaaa aaatgtcact ttcataaaag
     6241 ggctaatgcc cttttatgaa gctgactgct gcttctcgaa aaatagctca tataaaaatt
     6301 taagtgacag attataatcc tccatccacc agattgcttt ccattcatca ctgctgtgac
     6361 gcagctgaag gtgtggttct actgtttttg acactttttg gggaacttct gtattggcat
     6421 ttttgttctg aggtttcaga taacggatgg tattatagat tcgtccgccg tactctacac
     6481 gaattccaaa acgtgggtta attaatacag tgatctcacg gcgggcattg ataatcggaa
     6541 atccctcatc taggatttga aaacaccgat ttttaaacga aaatgtcccg gctgcatctg
     6601 tttttctcgt gtgctttaca caaaggatgg aatccagatt caaatctttt gcggcagaga
     6661 caaaaataga ctctgcttcc ggctcaacgg caaacctctg attgaaaagt tccctgtact
     6721 ctgtttccag aaaacggttt gcttctgaca cagtagtaat cccacgtttt gcgaactcaa
     6781 cagggagcct gctctgcagt gtgacccata aacgttcaat ccgccccttt gcctgaggag
     6841 tctttgcgaa aataaggtca atgcccaatt cgtgcaggga acgtccgaac tgggtaagat
     6901 ggacagtctt gcctgcgatt agttcatcaa cagtcagctt tccggtttta ggagaacgga
     6961 agatggtatg gttgtcagaa taaaccgtct gtgggatgcc gaaatcagag cagcattgac
     7021 gcatggtttc cagataaccg taaaggcatt cattctgtgt caggaaaagt ccgacgacct
     7081 gcccggtagc atcatcgata gcaccatgaa gggcgtattt gatatttccc ccaaaccact
     7141 cgtaaggcgt ggcatcgatc tggatgagct gtccgggatg gggcttacgt ttgcgccgat
     7201 gtgtgcggtg tcggattttt ttctttttag ggctttcaaa accagcattt tttagtatac
     7261 tggaaagagc agagtaggaa agttgaattc cgaaatctgc agagagtata tctttgaaat
     7321 ggaggaagtt ggcattagag aattctgggc gggaataaat cttaaggaca gcttccttca
     7381 attcgtcaga aacggagtga acgggttttc taccggtatt tttatgaatg agggattgag
     7441 cgccagaggt aaggactcct ttctttaagc gtgtgatctg gcgtgtggag agacccaaaa
     7501 gttcagcagc ctgttgtctg gaaatagact tgtcaatgaa acggttgatg acagtcagcg
     7561 ttttcagttg ttcttgtgac aatgtaattt taccttcttt catagcttta atagaatacc
     7621 aaaaagagac aaaacctcaa gtgaatctta aggagacatt atcacaagtt aataaca
//
LOCUS       DBMDXF010000086         7603 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2531_length_7603_cov_2.157025, whole genome shotgun sequence.
ACCESSION   DBMDXF010000086 DBMDXF010000000
VERSION     DBMDXF010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_7603_cov_2.157025"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..4309
                     /locus_tag="ACZPD4_04800"
     CDS             <1..4309
                     /locus_tag="ACZPD4_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIR1378425.1"
                     /translation="VQHIFETPVYMKSFKMTNFDLQIDIEGQNGYNNISILEVRAYSN
                     DQSETPQGQTLDEVVASIKGTKIAKDVNEFTLPNVPDGFTIESNGADFEQIIGEADKE
                     TGKLPVVHPMTDKEVQISFNVTETKTGNVKNTGDLAFTVEGTKDTTKAKNAKPSVIPE
                     IQEWFSESDQKVSVDTLTEVTYSDDSLKAIVDEFVSDYKDFTGKELKAGKSSEGKANA
                     FNFKKAAPDELLGDEGYTMDIKSDRIDVQSVSVTGNMYGMQTILQMYKGSEDGGYSIG
                     TMRDYPRYETRGFLLDVARKPVSLEMMKEITRTMRYYKMNDFQAHLSDNYIWLGEYGK
                     NGTENNAFSAYEAFRLESGLTNEAGESPTAKDYSITKEQFKKFIADERAVGMNIVPEI
                     DVPAHALSFTKIWPELKVSNKLHNNNPLIDHFDLTNDAAVTKIKEIFDDYTKGTNPTF
                     DADTVVHIGADEFMADAKSYREFVNEIIPYVDKTNTVRMWGGLTQIKDNPPTEINKDA
                     IDGVQINLWSKDWADGIDMYNMGYDLINTIDDHGYLVPDGSLTRKNAYGDLLDIGRIF
                     KDFKVNNVRTKNGAYKAVPSGDDQMLGAAFALWSDNIDRKASGLSESDLYWRFFDAMP
                     FYAEKTWAATGQEKGTSAKLTALAEKMGTGPNTNPYYQEEKKGEEYEKYEFADMKDSS
                     ENKRDLKEGKGAEVKDGVLNLGDEESYVTTPIEQLGNGNAISFDLELNKPSKPGDILF
                     EADPAYGTHDIRVMENGKLGFTRELYDYYFDYELPVGKKVNLKIVSTQQKTELFADGR
                     SVGTAVGKFVHNGQVKKEGIKNATFALPIERIGSKTNALAGKIDNVVVSAVNTEDKYN
                     KKDWTITTDSEYSNNSATEGEVTKAFDGKADTKWHSEWQAGAGETNGRLHDGAPTGQG
                     TVDTIFAQAKFDKGYEIDRIAFTPRQDQPSGYVTKADLYIQTEKDGEMKKVVTDAQFA
                     ADKTQKVFRFNKQTVYGFKFVAKASNDGWVAVSEFNVGDEIQVVESGKNTIFVNAEKG
                     GKAEIVGAEAGKPFEVDKGAKVTVKATPDKDYHFVGWYHSSNAETPVSTDAEYEFTVS
                     GNYALTAKFEKDNVTPPVETKDVTSIDKPEKVTVEEGTTFEDLKKDLPKTVTVHFGEG
                     EKAETADVEVTWEKGEYKGEAEKTYTLSGTLGKLPENVTNTKNLKAEIEVEVTKKEVT
                     PPVETKDVISVEKPEKVTVEEGTTFEDLKKDLPKIVTVHFGEGEKAETADVEVTWEKG
                     EYKGEAEKTYTLSGTLGKLPENVTNTKNLKAEIKVEVTKKEVTPPVEKEEYTITVSVN
                     DSKMGTVKLNPEKDSYKEGDVVTATAAAKEGYKFVNWTVDGKAVSDKAEFTFKVDRTL
                     TLKANFEKAAENPNQEDPKDDDKAVQTGDTAGSPIVPIAGLALAAGAVMAAVRKKED"
     gene            complement(4449..5780)
                     /locus_tag="ACZPD4_04805"
     CDS             complement(4449..5780)
                     /locus_tag="ACZPD4_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR1378426.1"
                     /translation="MDSGDSFQLIILIILLVLSAFFSSSETALMSVNKIRLRSLAESG
                     NKRAALALDIVENKTSKMLSAILIGNNIVNLSASAIATSLAYSFGGYMVSIATAVLTV
                     MILVFGEITPKNYATMNTEKLTLKFIPIIRFFMIVLTPFIFIINLFSRAFMFLLRIDP
                     NAASTAMTEDELRTIVDVSHEDGVIESDEKEMIYNVFDLGDANAKDIMVPRVHVTFAE
                     VNNTYEELIAIFREDKFTRLPVYEDTPDNIVGIINMKDLLLYNKKTETFNIRDIMREP
                     HFTYEYKDISELLVEMRDSTFNIAIVLDEYGEMAGLITLEDILEEIVGEIHDEYDEKE
                     DDMFRQISEQEYIIEGSMNLDDINDRLETDLVSEDYDSLGGFIIEHLDRLPEVGDEVC
                     TDNGIRLIVEALDKNRVESVRMYLPEKHDADNKSELSSSESAAQTAFTEEK"
     gene            6078..6983
                     /gene="murB"
                     /locus_tag="ACZPD4_04810"
     CDS             6078..6983
                     /gene="murB"
                     /locus_tag="ACZPD4_04810"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1378427.1"
                     /translation="MELYKALCEISGEGNVLKDEPMKMHTTFRIGGPADYFIQPSKIE
                     EIRRIVAVCRECSTPYYIMGNGSNLLVGDKGFRGVIVQVFKQMSDVYADGERVYAQAG
                     ALLSKTAAVACEASLTGMEFASGIPGTLGGALRMNAGAYGGEMKQIVESAEVLTSEGE
                     VLNLTNEELGMGYRTSVIAKNDYVALSAVLKLEKGDRQEIRAYMEDLKERRVTKQPLE
                     FGSAGSTFKRPEGYFAGKLIQDSGMQGYRVGDAQVSEKHCGFVINRGNATAAEVMQLM
                     KDVADRVEEKFGVRLEPEVRRIGEF"
     gene            6986..>7603
                     /gene="rapZ"
                     /locus_tag="ACZPD4_04815"
     CDS             6986..>7603
                     /gene="rapZ"
                     /locus_tag="ACZPD4_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1378428.1"
                     /translation="MRFVIVTGMSGAGKSTALKMLEDMGYFCVDNLPIPLLPRFVEMF
                     SEPDEEVKKIALGIDIRGGQDFGGLKDVLDEMDVKEIEYEILFLDAQDDVLIKRYKET
                     RRQHPLSGSGRVDTGIAKEREKIMFLKMRATYILDTSKMLTRELKLELEKIFVKGQNF
                     CNLYITVMSFGFKYGIPSDSDLVFDVRFLPNPYYIDGLREKTGNDP"
BASE COUNT         2493 a         1293 c         1968 g         1849 t
ORIGIN      
        1 tgtacagcac atttttgaga cgcctgttta tatgaagagc ttcaagatga cgaactttga
       61 tcttcagatt gacattgaag gacagaacgg atataacaac atcagtattc tggaagtgag
      121 agcatattcc aatgatcagt ctgagacgcc gcagggtcag acacttgacg aggttgttgc
      181 ttcgatcaaa ggcactaaga ttgcaaaaga tgtaaatgaa tttacactgc cgaatgtacc
      241 ggacggattt acgatcgagt cgaacggagc tgattttgag cagatcatcg gagaggcgga
      301 taaagagaca ggaaaacttc ctgttgttca tccgatgacg gacaaagaag tgcagatttc
      361 tttcaatgta actgagacaa aaacaggaaa tgtgaaaaat accggagatc ttgcatttac
      421 ggtagaaggt acgaaagata ctacaaaggc gaaaaatgcg aaaccttcgg ttattccgga
      481 gatacaggag tggttttcgg aatccgatca gaaagtgtcg gttgatacac ttacggaagt
      541 aacatacagt gatgattcat taaaagcgat tgtagatgaa tttgtatcgg attataagga
      601 ttttacaggc aaagagttaa aagcaggcaa gagcagcgaa ggaaaggcaa atgcctttaa
      661 ctttaagaaa gccgcgcctg atgagcttct tggcgacgaa ggttatacga tggatatcaa
      721 atccgacagg atcgacgtac agtctgtctc tgtgacaggt aatatgtacg gtatgcagac
      781 gatccttcag atgtataagg gaagtgaaga cggcggatat agtatcggta cgatgcgcga
      841 ctatccgaga tacgagacga gaggatttct tttagacgtt gcccgcaagc cggtttctct
      901 tgagatgatg aaagagatca caaggacgat gcgttattat aagatgaatg attttcaggc
      961 gcatttgtcg gataactata tctggcttgg cgaatacgga aaaaatggaa cggaaaacaa
     1021 tgcgtttagt gcgtacgagg catttcgtct tgaatccgga ttgacaaacg aagcaggaga
     1081 aagtcctact gcgaaagatt actcgattac aaaagaacag ttcaagaaat ttatcgcaga
     1141 cgaacgcgcg gtaggaatga atattgttcc ggagatcgac gttccggcgc atgcattgtc
     1201 atttacaaag atctggccgg aattgaaagt gagcaataag cttcataaca acaacccgct
     1261 gatcgatcat tttgatctga caaacgacgc tgcggttacg aagatcaagg aaatttttga
     1321 cgattataca aagggaacga atccgacttt cgatgcagat acggttgttc acatcggagc
     1381 ggatgagttt atggcggatg caaaatcata cagagagttt gtcaacgaga tcataccata
     1441 tgtagacaaa acgaataccg tccgtatgtg gggcggcttg acgcagatca aagacaatcc
     1501 gccgacagag atcaacaaag acgctattga tggcgttcag atcaatcttt ggtcaaaaga
     1561 ctgggcagac ggaatcgata tgtacaatat gggatatgat ctgatcaata cgatcgacga
     1621 tcacggatat ctcgttccgg acggaagtct cacaagaaaa aatgcttacg gagatttgct
     1681 ggacataggc cgtattttta aagactttaa agtaaataat gtaagaacga aaaacggagc
     1741 ttataaagcg gttccttcag gagacgatca gatgctcggc gcggcatttg ccctgtggag
     1801 cgacaacatt gacagaaaag cttcgggact ttccgagtct gatctgtatt ggagattctt
     1861 cgatgcgatg ccgttctacg cagagaaaac ctgggcggca acaggacagg aaaaaggaac
     1921 ttcggcaaag ctgacagctc ttgcagagaa gatgggaaca ggtccgaata cgaatccgta
     1981 ttatcaggag gagaaaaaag gcgaagagta tgagaaatat gagttcgctg atatgaaaga
     2041 cagttcagag aataaacgag atctgaaaga aggaaaaggc gcggaagtca aagatggcgt
     2101 attgaatctt ggcgatgaag aaagttatgt cacaactccg atcgagcagc ttggaaacgg
     2161 caatgctatt tcctttgatc ttgaactgaa caagccttcc aaaccgggag atattctgtt
     2221 tgaggcagat ccggcgtacg gaacgcacga tatccgggtg atggaaaacg gaaaattagg
     2281 atttacaaga gagctttacg attattattt cgattatgaa cttccggtag gaaagaaagt
     2341 caatctgaaa attgtttcta cacagcaaaa gacagaatta tttgcagacg gtcgatccgt
     2401 aggaacggct gtcggaaaat tcgtgcataa cggtcaggtg aagaaagaag gaattaaaaa
     2461 cgctacgttt gcgcttccga ttgagcgaat cggatctaag acaaatgcgc ttgcaggaaa
     2521 gatcgacaat gttgttgtat cggcagtgaa tacagaagat aagtataata aaaaagattg
     2581 gactattact acagactcag aatattccaa caattctgcg acagaaggtg aagttacaaa
     2641 ggcgtttgac ggaaaagcgg atacaaagtg gcactcagag tggcaggcgg gagcaggaga
     2701 gacaaacgga agacttcatg acggagcgcc gacaggtcag ggtacggtag atacgatatt
     2761 cgcacaggca aagtttgaca aaggttatga gatcgacagg atcgcattta caccgagaca
     2821 agatcagcca agcggatatg taacaaaggc agacttatat attcagacag agaaagacgg
     2881 agaaatgaag aaagttgtca cagacgcaca atttgcggca gataagacgc agaaagtatt
     2941 ccgttttaat aagcagactg tatatggatt caagtttgtt gcgaaagcgt ccaatgacgg
     3001 atgggtagca gtgtctgaat tcaatgtagg cgatgagatt caagttgtcg aatccggaaa
     3061 gaatacaatt ttcgtaaatg cagaaaaagg cggaaaagcc gagattgtcg gagcagaagc
     3121 aggaaaaccg tttgaggtgg ataaaggagc gaaagtaacg gtaaaagcaa ctcctgataa
     3181 agattatcat tttgtaggat ggtatcattc atctaatgca gaaactccgg tttctacaga
     3241 tgcagagtac gaatttacgg tatcgggaaa ttatgcgctg acagcgaaat ttgaaaaaga
     3301 taatgtaacg ccgccggttg aaacaaaaga tgttacatct atcgacaaac cggaaaaagt
     3361 aacggtagaa gaaggaacaa catttgaaga tcttaagaaa gaccttccga agacagtgac
     3421 cgtacatttc ggagaaggcg agaaagcaga gacggcagac gtagaagtga catgggaaaa
     3481 gggagaatat aaaggagaag cagaaaagac atatacgtta agcggaacac ttggaaaact
     3541 tcctgaaaat gttacgaata cgaagaatct gaaagctgag atcgaagttg aagtgacaaa
     3601 gaaagaggta acgccgccgg ttgagacaaa agatgttata tccgttgaaa aaccggaaaa
     3661 agtgacggta gaagaaggaa caacatttga agatcttaag aaagaccttc cgaagatagt
     3721 gaccgtacat ttcggagaag gcgagaaagc agagacggca gacgtagaag tgacatggga
     3781 aaagggagaa tacaaaggtg aagcggaaaa gacatatacg ttaagcggaa cacttggaaa
     3841 acttcctgaa aatgttacga atacgaagaa tctgaaagct gaaatcaaag ttgaagtgac
     3901 aaagaaagag gtaacgccgc cggtggaaaa agaagagtac acgattacag tcagtgtaaa
     3961 cgattcgaag atgggaactg tgaagcttaa tccggaaaaa gacagctata aagaaggaga
     4021 cgttgtaact gcaacggcag cggcaaaaga aggatacaaa ttcgtgaact ggacggtaga
     4081 cggaaaagca gtttcagata aagcggagtt tacgtttaaa gttgacagaa cgctgacttt
     4141 gaaagcgaac tttgaaaaag cagcggaaaa tccgaatcag gaagatccga aggatgatga
     4201 taaagcagtg cagacaggag acacggcagg atcaccgatc gttccgatcg caggactggc
     4261 gttggcagca ggcgctgtta tggcagctgt tagaaagaaa gaagactgat gctgtgcaag
     4321 tgattggtta tcgctgatat aataagattt aaaagaaaga cataataaaa attccgtatc
     4381 aagatcggta agatcgagat acggaatttt tatatgctca tttatattcg atataggtat
     4441 aagatgtatt atttttcctc ggtgaaagct gtctgtgcgg cgctttcaga ggaagaaagt
     4501 tcgcttttgt tgtcagcatc atgtttttct ggaagataca tacgaacaga ttctacccgg
     4561 tttttatcaa gcgcctccac gataaggcga attccgttat cggtacatac ttcatctccg
     4621 acttcaggga ggcgatccag atgttcgatg ataaatccgc cgagggaatc gtaatcttcg
     4681 gatacaagat ccgtttccag acggtcgttt atatcgtcca gattcataga tccttcgatg
     4741 atatattctt gttcggaaat ctgtcggaac atatcgtcct ctttttcgtc gtattcgtca
     4801 tgaatctcgc ctacgatttc ttccagaata tcttccagtg tgatcagtcc tgccatttcg
     4861 ccatattcgt caaggacaat ggcgatgtta aaagtagaat cccgcatttc cacaagaagt
     4921 tcggaaatat ctttgtattc atatgtgaaa tgcggctcgc gcataatatc acgaatgttg
     4981 aacgtctctg tctttttgtt atagagcaga agatctttca tattaataat accgacgata
     5041 ttgtccggag tatcttcata aacaggaaga cgtgtgaact tatcttcgcg gaaaatggcg
     5101 atcaactctt cgtaagtatt atttacttct gcgaatgtaa cgtgaacgcg gggaaccata
     5161 atatcttttg cgttggcatc gccgagatcg aacacgttgt agatcatctc tttttcgtct
     5221 gattcgatta ctccgtcttc gtgacttaca tcgacgatcg tacgaagctc gtcctctgtc
     5281 attgcggttg aagctgcgtt cggatcgata cggagcagaa acataaatgc gcgcgaaaaa
     5341 aggttgatga tgaaaataaa cggagtcaaa acgatcataa agaatcggat gatcggtata
     5401 aattttagag ttaatttttc cgtgttcatt gtagcgtaat ttttaggggt aatctctccg
     5461 aagacaagga tcataaccgt taaaacagca gtggcaatgc tgaccatata accgccgaat
     5521 gaataggcga gagaggtagc gattgccgat gcggaaaggt tgacgatatt gtttccgatc
     5581 agaatggcgc tcagcatttt ggaagtttta ttctccacaa tatcaagagc cagggcggcc
     5641 cgcttgttgc ctgattcggc aagggaacgc agccgtattt tatttacgga catgagcgct
     5701 gtctcgcttg atgaaaaaaa tgctgaaagc actaaaagaa ttataagtat aattagttga
     5761 aaactgtcac cagaatccac agttctaaat tcactccttt ttaataaaaa ataaaataag
     5821 ccattttatg gcatgcaaaa atatagatac ctcatcagta tacactcttt tggagaaatt
     5881 tgcaagaatc ggataataat gacaaggagc ggataataat gacaaggaac ggagaagaga
     5941 atccgataat atcagtaaaa tcggatatgc ggcggtaatc ggtgacgttt cgctttactt
     6001 tgcattaggc gtgaattata atgtatcatt ataatgaaga taattaatat ataaaaaaca
     6061 gaaatatgga gatatttatg gagttgtata aagcattatg tgaaatatca ggagaaggta
     6121 atgtattaaa agacgaaccg atgaagatgc atacgacatt tcgcattggc ggaccggcag
     6181 attattttat tcagccgtcg aagatagaag agatccgccg gatcgttgct gtgtgccggg
     6241 agtgctctac cccatattat attatgggaa acgggagtaa tctgcttgta ggtgacaaag
     6301 gatttcgggg agtgatcgta caggtcttta agcagatgag cgatgtgtat gcagacggag
     6361 agcgcgttta tgcacaggcg ggagctcttt tatcaaaaac ggctgctgtt gcgtgcgagg
     6421 cgtcgcttac agggatggaa tttgcatcgg gcattccggg aacgctcggc ggcgcgcttc
     6481 gtatgaatgc gggagcatac ggcggagaga tgaaacagat cgtcgaaagt gcggaagtgc
     6541 ttacgtctga aggcgaagtt ttaaatttga cgaatgaaga acttggtatg ggatatagaa
     6601 caagtgttat tgcgaaaaat gattatgtgg ctctgtctgc ggttttgaaa ctggagaaag
     6661 gagatcggca ggagatccgt gcatatatgg aagatttaaa ggaacgacgc gttacaaagc
     6721 agccgcttga atttggaagc gccggcagta cgtttaagcg cccggaaggg tattttgcgg
     6781 gaaaactgat acaggattcc ggtatgcagg gatatagagt cggagacgct caagtgtctg
     6841 aaaaacactg cggatttgtt atcaacagag gaaatgctac ggcagcggaa gtcatgcagt
     6901 tgatgaaaga tgttgcggat agagtggaag agaagttcgg ggtacggtta gaaccggaag
     6961 taagaaggat aggagagttt taacaatgcg ttttgttatc gtgacgggaa tgtcaggggc
     7021 aggaaaatcg acagcgctta aaatgttgga ggatatgggg tatttttgtg tagacaatct
     7081 gccgatcccg cttcttccgc gttttgtgga gatgttcagc gaaccggacg aagaagtgaa
     7141 aaagattgcg ctcgggattg atatcagagg aggacaggat ttcggaggac tgaaagatgt
     7201 tcttgatgag atggatgtca aagagatcga gtacgagatt ctttttcttg acgcgcagga
     7261 cgatgtgctt attaaacggt ataaagagac aagacgtcag catccgttga gcggttccgg
     7321 gcgtgttgat acagggattg caaaagaacg agaaaagatc atgttcttga agatgagagc
     7381 gacttatata ttggatacaa gtaagatgct tacgagagaa ttgaaactgg aacttgagaa
     7441 gatttttgtg aagggacaaa acttttgtaa tctttatatt acagttatgt catttggttt
     7501 caagtatggg attccaagcg attcggatct cgtatttgac gtgcgttttt tgcccaatcc
     7561 ttattatata gacggacttc gggagaagac gggaaatgat ccg
//
LOCUS       DBMDXF010000087         7448 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2587_length_7448_cov_1.639853, whole genome shotgun sequence.
ACCESSION   DBMDXF010000087 DBMDXF010000000
VERSION     DBMDXF010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2587_length_7448_cov_1.639853"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..1146
                     /locus_tag="ACZPD4_04820"
     CDS             55..1146
                     /locus_tag="ACZPD4_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1378429.1"
                     /translation="MISNQILQNTIDGLKGISKVDFSVLDTEGKELASTLDNAQERNS
                     AVISFVESPADSQVIQGCQFFKIFDEQRLEYVLLAEGDSETAYMLGKVAAFQIHSLLV
                     AYKERFDKDNFIKNLLLDNLLLVDIYNRAKKLHIDTEVKRVIFIIETSHEKDNAALEN
                     VRNLLGGKSKDFITAVDEKNIIVVKELTEKDGAKELEKMAKEMLDMLKEDTKDEQIRI
                     AYGTIISDIKEVSKSYKEAKLALDVGKIFFAEKDIVAFTTLGIGRLIYQLPIPLCKMF
                     IREIFEGKSPDDFDEETLTTINKFFENSLNVSETSRQLYIHRNTLVYRLDKLQKSTGL
                     DLRVFEDAITFKIALMVVKYMKYMESLEY"
     gene            1181..1867
                     /gene="ftsE"
                     /locus_tag="ACZPD4_04825"
     CDS             1181..1867
                     /gene="ftsE"
                     /locus_tag="ACZPD4_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1378430.1"
                     /translation="MIELRNVTKEYSKGNSALNGVSVKIERGEFVFIVGDSGSGKSTL
                     IRLIMKELNPTEGTIIVNGQNLNRMKHRNIAKYRRGLGVVFQDFRLLRDRNIYENIAF
                     ALRVTETPTKVIKKKVPAALSLVGLAQKYKSFPKELSGGEQQRVAIARAIVNEPAILL
                     ADEPTGNLDPTNSWEIMSLLKEANERGTTVLVVTHNQEIVNEMNERVITMKQGVIVSD
                     EQKGGYIDED"
     gene            1857..2807
                     /gene="ftsX"
                     /locus_tag="ACZPD4_04830"
     CDS             1857..2807
                     /gene="ftsX"
                     /locus_tag="ACZPD4_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR1378431.1"
                     /translation="MRISTFGYVGKQGVKNIWRNKMFSLASIATMSACIFLFGLFFSI
                     IVNFQHIIKTAEEGVAITVFFEEDATDSEKKAIGKALEARKDVAEVKYISAEEAWEEF
                     QKQYFADNPELAEGFKNDNPLASSDNYEVYMKTAEDSKSLTARSRSLSETQKELVSYA
                     QKLDGVREVNKSDVVAKMLSSVNTLVAVISIAIIIILLGVSIFLISNTVTMGITVRKE
                     EIAIMKYIGAKDFVVRSPFVIEGLIIGIFGAAIPLVLLYFLYDKAVQYIMVKFSILEN
                     IITFLPVSTVYQILLPIGVVMGIGIGFLGSFFTVRKHLKV"
     gene            2820..3914
                     /locus_tag="ACZPD4_04835"
     CDS             2820..3914
                     /locus_tag="ACZPD4_04835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1378432.1"
                     /translation="MKCRMKRVASLFLALALCAGMTMNVGAVTVQEAENKADELEGKK
                     EQAEAEAKDLTEKLKGIVADMEETQKKLTEKEEEIEQVENELVQAQIDANDQYERMKV
                     RIKYMYESGNTQFVAVLAESKNMGDFLNKAEYISQISEYDRDELIRYQDTVEEIEVKE
                     EEVQAEYEELNTLQTKLVGQQTEVQKMIDENKEKLSNIQSEIDANAAALEKAKEVERI
                     QQEQAGNNYIPSTGGNVVSGNGYFTHPCPGMSYQSSYFGEIRPYEVGGHKGHDYAAAV
                     GTPTYAAAAGTVVIAGFSYSAGNWVVINHGNGLVTKYMHHSALAVRAGQYVEKGQQIG
                     YVGSTGQSTGPHLHFQVELNNVPVNPDLYM"
     gene            4184..5476
                     /locus_tag="ACZPD4_04840"
     CDS             4184..5476
                     /locus_tag="ACZPD4_04840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1378433.1"
                     /translation="MDQTSNNQNESDEIKMSRGSVDANEGSLGSGKKKSRFGIGVLVG
                     IVIGAVIITGVWGAAKVVSAVFFKGNFATESTSRSDMEHKLDRLNALIEKYYLYEDEI
                     DKDALVEGIYSGYAGALGDPYTEYYDEQEAKELFETTNGEFFGIGASMSKQLGTDEIT
                     IVNVYEDSPADKAGLKSGDILYKVDEHETAGYELDTVVSWIKGEKGTSVVLHVLRDGK
                     EVEVTAVRDKIEVQTVAYEMKEDQIGYIAVSEFDSVTYHQFETALEDLEKQGMKGLVI
                     DLRNNPGGNFDTVTDMLKLLLPEGVIVSTKDKEGNVEEVTCDGANEFDKPLAVLVNQY
                     SASASEIFSGAVQDYGIGEIVGMTTYGKGVVQQLMDLGDGTCLKMTIAEYYTPNGRSI
                     NGKGVEPDVEVEYEYDENNPEADTQLEKALEVVKDQNK"
     gene            complement(5660..6247)
                     /locus_tag="ACZPD4_04845"
     CDS             complement(5660..6247)
                     /locus_tag="ACZPD4_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1378434.1"
                     /translation="MEKHWYDYLWIVSLAYLILGFFNILFAWIGLLCFFIPLIISLAN
                     GTKGYCNRYCGRGQLFGLIGGRFGLSRKKDVPLWMKSKWFRYGFLIFFFVMFFQMLWN
                     TGLVFAEIHSLKQAVSLLWTFSFPWDWAYHGTLFHPGVAQFAFGFYSVMLTSTVLGVA
                     TMILFKPRSWCVYCPMGTMTQLICKAKSPKLNDSK"
     gene            6455..6724
                     /locus_tag="ACZPD4_04850"
     CDS             6455..6724
                     /locus_tag="ACZPD4_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378435.1"
                     /translation="MDILIVMCIGILVGRVSILRRAKKKNEYLSLFCTFILIFSMGVM
                     LGKKDHFFEELSSLGLTSFLFFLIPTVLSIGLVYWLTRVFMKKDK"
     gene            6766..7368
                     /locus_tag="ACZPD4_04855"
     CDS             6766..7368
                     /locus_tag="ACZPD4_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HIR1378436.1"
                     /translation="MIVFLTILSLVSGLVYGYFDVESSFISAITQNTHIVLYILMFSV
                     GISIGMYDGIVQKIREYHIKIFIIPIGIIIGSLLGGIICSMIVKMPVGHGTAIASGLG
                     WYSLSGATISKLVSAEAGSIAFLSNLMREIFSFFIIPFLAVHFNYYTCIAPAGATSED
                     TTLPVMLKYTNEETVVLSVLNGVICSFCVPILISFCLNIK"
BASE COUNT         2393 a         1222 c         1880 g         1953 t
ORIGIN      
        1 aaaaaaaaaa aaaaaaatgt acatgatata aatccaagga gtggaggaac aggcatgatt
       61 tcaaatcaga tacttcagaa tacgatcgac ggattgaaag gaatttccaa agttgacttc
      121 tcagtattag atacagaggg aaaggaactg gcatctacat tagacaatgc gcaggaacga
      181 aacagcgcgg taatctcgtt tgtagaatcg cctgcggaca gtcaggtaat acagggatgt
      241 cagtttttta agatttttga cgagcagagg cttgagtacg tactgctggc ggaaggtgac
      301 agcgagacgg cgtatatgct cggcaaagtt gcggcattcc agatccacag tctgctggta
      361 gcttataaag aaaggttcga caaagataat tttattaaaa atcttcttct ggacaacctt
      421 ctgttggtag acatttataa ccgtgctaaa aaattacata ttgacacgga ggtaaaacgt
      481 gttatcttta ttattgagac atcacacgaa aaggataatg ccgcgcttga gaatgtgaga
      541 aatcttcttg gcggaaaatc aaaagatttt attactgcgg ttgatgagaa gaatatcatt
      601 gtagtaaaag aactcacaga aaaagacggg gcgaaagagc ttgagaagat ggcgaaggaa
      661 atgctcgaca tgctgaagga agatacaaag gacgagcaga tccgtattgc atacggtacg
      721 atcatcagcg acatcaagga agtatccaag tcctataaag aggcgaagct tgcgcttgac
      781 gtagggaaaa ttttctttgc ggaaaaggat attgttgcat ttacgacact tggtatcggc
      841 cgtctgatct atcagctccc gattccgttg tgcaagatgt ttatcagaga gattttcgag
      901 gggaaatcgc cggatgattt tgatgaggag acattgacaa cgatcaataa attttttgag
      961 aacagtctga atgtatcgga aacatcccgg cagttgtaca ttcacagaaa tactcttgta
     1021 tacagactgg ataaacttca gaagagcacg gggcttgatc tgcgtgtgtt tgaggatgcg
     1081 atcacgttca agatcgcgct tatggttgta aaatatatga aatatatgga atcattagag
     1141 tattagatct tacaggagta taaataatta ggaggaactt atgattgaat taagaaacgt
     1201 gacgaaagag tactctaagg gcaattcagc tcttaacgga gtctctgtga aaatcgaacg
     1261 gggagaattc gttttcatcg tcggagacag cggctcggga aagtctaccc tgattcgttt
     1321 gatcatgaag gagttgaatc cgactgaggg aacgatcata gtaaacgggc agaacctgaa
     1381 tcgtatgaaa caccgcaata tcgcgaaata cagaagagga ttgggcgttg tgtttcagga
     1441 cttccgtctt ttgagagaca gaaacattta tgaaaatatt gcgtttgccc tgcgcgtgac
     1501 ggaaacgccg acaaaagtga tcaagaaaaa agtgcctgcg gcgctttcgc tggtcggtct
     1561 tgcgcaaaaa tataaatcgt ttccgaagga attatcaggc ggagagcagc agcgagttgc
     1621 aatcgcaagg gcaatcgtaa acgaacccgc tattttgttg gcagacgagc ctacgggaaa
     1681 cttagatcca acgaactcat gggagatcat gagccttttg aaagaggcga atgaaagagg
     1741 aacgacagtg cttgtcgtta cgcataacca ggagattgta aacgagatga acgagcgcgt
     1801 gatcactatg aaacaaggtg tgatcgtcag tgatgaacaa aaaggtgggt atatagatga
     1861 ggattagtac atttggatac gtcggaaaac aaggcgttaa gaatatctgg agaaacaaga
     1921 tgttttcgct cgcgtccatt gcgacaatgt cagcttgtat cttcttgttt ggattattct
     1981 tttcgattat tgtaaatttt cagcatatta taaaaacagc ggaggaaggc gttgcgatca
     2041 cagtattctt tgaagaagac gctacagatt cagagaagaa agcgatcggg aaggcgctgg
     2101 aagcccgcaa agatgtggca gaagtaaagt acatatcggc agaagaagca tgggaagaat
     2161 tccagaagca gtattttgca gacaatccgg agctggcaga aggatttaaa aacgataacc
     2221 cgctggcatc ttcggacaac tatgaagtat atatgaaaac agcggaagac agtaagagtc
     2281 ttacagcgag aagccgttct ctttcggaga cgcagaaaga acttgtttct tatgcacaaa
     2341 aactggacgg cgtacgagaa gtaaacaagt cggacgtagt agcgaagatg ctttcaagcg
     2401 taaatacact tgtagcggtt atttcgatcg caattatcat tatccttctc ggagtttcga
     2461 tcttcctgat cagtaatacc gttacgatgg gtattaccgt gcgaaaagaa gagattgcga
     2521 tcatgaagta catcggagcg aaagactttg tcgttcgttc tccgttcgtg atcgaaggtc
     2581 ttatcattgg tattttcggc gcggcgattc cgcttgtact gctgtacttc ctgtatgata
     2641 aagcagttca gtacattatg gtgaaattca gtattcttga gaatattatt acattccttc
     2701 cggtgtcgac tgtatatcag atcttactcc cgatcggtgt ggtaatggga atcggaatcg
     2761 gtttcctcgg aagtttcttt acggtgcgca aacatctgaa agtataggag gtatactcga
     2821 tgaagtgtag aatgaagagg gtggcgagtt tatttcttgc gttggcgctg tgtgccggaa
     2881 tgacgatgaa cgtaggggct gttaccgttc aggaagcgga aaataaagcg gacgagcttg
     2941 aaggcaaaaa agaacaggct gaggcagagg cgaaagatct cacggaaaaa ttaaaaggga
     3001 tcgttgccga tatggaagaa acgcagaaga aactgacaga aaaagaagag gagatcgaac
     3061 aggttgaaaa tgaacttgtt caggctcaga ttgatgcgaa tgatcagtat gagagaatga
     3121 aagttcgtat caaatatatg tatgagagtg gaaatactca gtttgtagcc gttcttgccg
     3181 agagtaagaa tatgggagat tttctgaata aggcagaata tatttctcaa atctcagagt
     3241 atgacagaga cgaacttata aggtatcagg atacggttga agaaatcgag gtgaaagaag
     3301 aagaggttca ggcagaatat gaggaactga atacgcttca gacaaagctt gtcggtcagc
     3361 agacggaagt tcagaaaatg atcgatgaga ataaagaaaa acttagcaat atccagtcgg
     3421 agatcgatgc gaatgctgct gcattggaaa aggcaaaaga agttgaaaga atccaacagg
     3481 agcaggcagg aaataattat attccttcaa cgggcggaaa cgtcgtgagc ggaaacggat
     3541 attttacgca cccgtgtccg ggtatgtcgt accaatccag ttattttggc gagatcaggc
     3601 catatgaggt cggcggccat aaaggacatg attatgctgc ggctgtagga acacctacat
     3661 atgcggctgc ggcaggaacg gttgtgatcg ccggattcag ctacagcgcc ggaaactggg
     3721 ttgttattaa tcacggaaac ggacttgtga caaaatatat gcatcacagc gcacttgccg
     3781 tcagagcagg gcaatatgta gagaaaggtc agcagatcgg atatgtggga agcacagggc
     3841 agtcaaccgg accgcatctt cattttcagg tggagctgaa taatgtacct gtaaatcctg
     3901 atttgtatat gtaaagtggt tatacatagg aatagccggg ggtaatgatt cagtcagtga
     3961 aatttactat atgggagatt tcccgtatat ggaagaagag gggagacaag gcagagtatg
     4021 atctgttttg tctttcttct gtatagacaa aaagtttggc gacatttcat aaaagtcgaa
     4081 tgggaaataa aaacggttat ataatagata gaggggcgtt ggaaagtaat gcgatgtgat
     4141 gtaagaaaat ttgcggatga acgaaaacag gggaatgaaa aacatggatc aaactagtaa
     4201 taatcaaaat gagtcggatg aaatcaagat gagtcggggc agcgtggatg caaatgaagg
     4261 ttctttaggc agcgggaaga aaaaaagcag attcgggatt ggcgttcttg taggaatcgt
     4321 gatcggcgca gttataatta ccggagtatg gggagctgca aaggtggtat ctgccgtatt
     4381 tttcaaaggc aattttgcaa cggaaagtac atcaagatca gatatggaac ataaacttga
     4441 caggcttaac gcgttgatag agaagtatta tctgtatgaa gatgagattg ataaagatgc
     4501 ccttgtagaa ggtatttatt cagggtatgc aggcgcgctg ggagatccgt atacagaata
     4561 ctacgatgaa caggaagcga aggagctttt tgaaacaacg aacggtgaat ttttcggaat
     4621 cggagcatcg atgtcgaagc agctcggaac agatgagatt acgatcgtca atgtgtatga
     4681 agattcacct gcagacaagg cgggcttgaa atcaggcgat atcttatata aggtagacga
     4741 acatgagacg gcgggatatg aactggatac agtcgtttcg tggatcaaag gcgaaaaagg
     4801 tacgtcggtt gtcctccatg tgctaagaga cggaaaagaa gttgaagtga cggctgtcag
     4861 ggataagatc gaagtacaga cagtcgcata tgaaatgaaa gaagatcaga ttggctatat
     4921 cgctgtaagc gagtttgaca gcgtaactta tcatcaattt gaaacggcgc tggaagatct
     4981 tgaaaagcag ggaatgaaag ggcttgtaat tgatcttcgt aataatcccg gcggaaattt
     5041 tgatacagta acagatatgc tgaagctgct tcttccggaa ggagtgatcg tgtcgacaaa
     5101 agacaaagaa gggaatgtag aagaagttac ttgcgacgga gcaaatgaat ttgataagcc
     5161 gcttgccgta cttgtaaatc aatacagcgc cagcgcgtcg gagattttca gcggagctgt
     5221 gcaggattac ggcatcggag agatcgtcgg tatgacgact tatggaaaag gggtcgttca
     5281 gcagttaatg gatcttggag acggaacatg cttaaaaatg acaatcgcag aatactatac
     5341 tccaaacgga cggagcatca acggcaaagg cgtagagcct gacgtggaag tagagtatga
     5401 atacgatgag aataatcccg aagcagacac ccagttagag aaagctttgg aagtagtgaa
     5461 agatcaaaat aaataaaaca aaaacaatat gggcggacaa tatggaacag aataagttta
     5521 acggaaaaat ctgagcagaa agtctgaaca tctgaacaga aaaagcggaa cagtgaactg
     5581 tccgcttttt tcgtaattat tcaaattgtt ttttaataag tttttaaatc ggttttttga
     5641 atcggttttt cgtgtgggct cattttgaat cgtttagttt tggacttttc gccttacaga
     5701 tgagctgagt cattgttccc atcggacagt aaacgcacca gcttctcggt ttgaacaaga
     5761 tcattgttgc aaccccaagt acggtagatg tcagcatgac actgtaaaat ccaaaagcaa
     5821 actgtgcgac gcccggatgg aatagtgttc cgtggtatgc ccaatcccac gggaacgaaa
     5881 atgtccagag cagggagact gcctgtttta agctgtggat ttctgcaaat acaagtcctg
     5941 tattccaaag catttggaaa aacatgacaa agaaaaagat tagaaatcca tatctgaacc
     6001 atttactttt catccacaga ggcacgtctt tttttcggga aagaccgaaa cgtcctccga
     6061 tcagaccgaa aagctgccct cttccacaat accggttaca atatcccttt gttccatttg
     6121 caagagagat gatcaaaggg atgaaaaaac aaagaagtcc gatccatgca aacaggatgt
     6181 tgaaaaatcc gagaatcaaa taagcgaggg atacgatcca caaatagtca taccaatgtt
     6241 tttccatgac ggacctccta aaagtttgtt ctattaatat atagcataga ggatgataag
     6301 attctgtgat ataatcacgc aagcagatga caatgtgctt tttggcgctt ttcggtgatt
     6361 cctgaagtat ttgttttcct tttatacatg acgaaacagg aaaattttga tatagtatat
     6421 aagtgcgaaa aatcggattg tgaggagtta agaaatggat atattgattg ttatgtgtat
     6481 cgggatttta gtaggaagag tttcaatcct gcgcagggcg aaaaagaaaa atgaatatct
     6541 ttctttgttt tgtacgttta ttttgatttt ttctatggga gtcatgcttg gaaagaaaga
     6601 tcattttttt gaagaattat catcgcttgg tctgacaagc tttctctttt tccttatacc
     6661 gaccgtgctt tctatcggat tggtttactg gctgacaaga gtttttatga agaaagataa
     6721 ataggccgtt gtaaaaaata aagttttagg aaagaagggg tttagatgat cgttttttta
     6781 acgatattgt ccttagtatc aggacttgtc tacggctatt ttgatgtgga aagcagtttc
     6841 atttccgcaa tcacgcagaa tacccatatt gttttatata tattaatgtt ctccgtcgga
     6901 ataagcatcg gaatgtatga cggtattgtg cagaaaatac gggaatatca tattaagatt
     6961 tttattattc cgatcggaat tattataggc tctcttttgg gaggaataat ttgttctatg
     7021 atcgttaaaa tgcctgttgg tcatgggacg gcgatcgcga gcggattagg ctggtacagt
     7081 ctttcaggag caacgatcag caagctggta agcgcagaag ccggaagtat cgcttttttg
     7141 agtaatctga tgagagagat cttttcgttt tttattattc cgtttttggc ggtacatttt
     7201 aattattata cgtgcattgc acctgcggga gcgacaagtg aagatacaac gcttccggta
     7261 atgctgaaat atacaaatga agaaacggta gtgttatctg ttttaaacgg agtgatctgt
     7321 tcattttgtg tgccgatact tatttcgttt tgtttgaata taaagtaaaa atatatagtc
     7381 agaatataaa gtcaagatac gcaatgtttt tgccaataat gttgcaaaaa aatacctgct
     7441 gtatgata
//
LOCUS       DBMDXF010000088         7403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2604_length_7403_cov_1.743221, whole genome shotgun sequence.
ACCESSION   DBMDXF010000088 DBMDXF010000000
VERSION     DBMDXF010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_7403_cov_1.743221"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="ACZPD4_04860"
     CDS             complement(<1..400)
                     /locus_tag="ACZPD4_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR1378437.1"
                     /translation="MKAYGVNELRKMFLEFFESKGHLAMKSFSLVPHNDKSLLLINSG
                     MAPLKPYFTGQEIPPRKRVTTCQKCIRTGDIENVGKTARHGTFFEMLGNFSFGDYFKK
                     EALAWSWEFLTEVVGLDPDRLYPSVYEDDDE"
     gene            complement(588..2099)
                     /locus_tag="ACZPD4_04865"
     CDS             complement(588..2099)
                     /locus_tag="ACZPD4_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1378438.1"
                     /translation="MMRGLHTPVRAIRRRVFKEVAQLGFKADADTLLADMEAIPYAIV
                     NEDSEKYRESVYRARAIVRERLRLAMGLSLRPENKPVHLTAGVEASNISEKYYEPPLM
                     QVIPSACESCEENKYEVSNICKGCLAHPCQEVCPKDAISMVNGRSYIDQEKCIKCGKC
                     KSVCPYDAIAKKERPCQKACGVNAIKSDKMGRAYIDNEKCVSCGMCMVSCPFGAISDK
                     SQIFQLARALSEGENVIAEIAPAFVGQFGDNITPRNIKAALRELGFSEVYEVALGADI
                     GAIAEAHHYVDKVVTGELPFLLTSCCPSWSVMAKKFFPDLIDQISQELTPMVATARTI
                     KQKHPEAKVVFIGPCAAKKLEASRRSVRSDVDFVVTFEELQAMFDAKEIDLTEYEAES
                     SFHDATGAGRGYGCAGGVAEAIEKCINEYYPDVQVNIEHAEGLKECKKILTLAKAGKM
                     NGCLIEGMGCPGGCIAGAGTNIPLPKAKKDLAAFVKNSSKSIPPKELEEIELD"
     gene            complement(2219..4699)
                     /locus_tag="ACZPD4_04870"
     CDS             complement(2219..4699)
                     /locus_tag="ACZPD4_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1378439.1"
                     /translation="MIRQRLGKDLLFFDGGMGTLLQEKGLAPGELPETWNLTHSEEIY
                     KIHRQYIEAGSDIILTNTFGANALKFHDDSCSLEEIIKAAVSHVKKAEREALLQTGDE
                     RKIYTALDVGPTGKLLKPMGDLEFETAYEAFKEVVILGEQAGADLIHIETMSDTYELK
                     AAVLAAKENTSLPVFATVIFDERKKLLTGADVSSVVALLEGLGVDALGINCAMGPKEM
                     LPVLEELIKYSSVPIIVKPNAGLPKQRDGKTYYDVTEDEFAAYMEQIVRMGACVIGGC
                     CGTTPEHIRAMRKRCENAELVPVTEKEFTVVSSYGQSVILGEGSKIIGERINPTGKKR
                     FKQALKEHDLDYILREGITQQDQGAHILDVNVGLPDIDEPALMEEVVQELQSVVNIPL
                     QIDTVDEKAMEKALRIYNGKAMVNSVSGKKESMEKVFPLVKKYGGVVIGLTLDEDGIP
                     ADADGRVRIAEKIIKTAEKFGIKKKDIVIDALAMTISSEPEGAKVTLETLRRLRDEIG
                     VNTVLGVSNISFGLPCRPIVNAAFYTMAMLNGLSAGIINPSSEDMMKSWYAYHALMNL
                     DNNCEQYIQKYANSVVSTNIMKTSELSETKLKGENDRSRKSSSKMTLQEAIEKGLRDD
                     AGKITTDMIATEAPLDIINEELIPALNHVGDGFEKGTVFLPQLLMSAEAAKSAFSVLK
                     EKMEKSGEIREKKGRVILATVKGDIHDIGKNIVKVLLENYSFDVIDLGKDVSPEKIVE
                     TACEKQVPLVGLSALMTTTVVSMEETIKMLREKKPDCKVMVGGAVLNQEYADMIGADF
                     YGKDAMQSVHYAEKIFKDALQKSADKKE"
     gene            complement(4760..5401)
                     /locus_tag="ACZPD4_04875"
     CDS             complement(4760..5401)
                     /locus_tag="ACZPD4_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR1378440.1"
                     /translation="MEKCFQEAVRYLGYGKNPVDEATSRLVEKGFLELEKVSALRSVF
                     RIFEMNRIDEEKVAIGTLQISSKSLARNLNHCEKAVLFGATLGTGVDRLIARTSLTDM
                     ANAVVLQACAAAMLEEFCDEKQFEIGEELEKEGLYLRPRFSPGYGDFDIGFQKSFMQM
                     LDCAKAIGLTMTESFMMTPTKSVTAVIGASRIKERCPIAGCEVCDKKECIYRR"
     gene            complement(5410..6279)
                     /gene="metF"
                     /locus_tag="ACZPD4_04880"
     CDS             complement(5410..6279)
                     /gene="metF"
                     /locus_tag="ACZPD4_04880"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR1378441.1"
                     /translation="MKIIDRLKEDKIHISFEVFPPKTDAGFEKVIAATDAMAKLNPAF
                     ISVTYGAGGGTSKNTAKIASHIKDDLGVVSLAHLTCASSTIEEVRDVIENLKENGIEN
                     ILALRGDIPEGMPFPNGNRFRYAYELIEEIRKHGDFCIGAACYPEGHVENEHKAEDIR
                     YLKQKVDSGVDFLTTQMFFDNDIHYNFLYRIREAGINVPVLPGIMPMTSEKQMKRSQE
                     LSGTVFPRRFLALLDRFGSSAAAMEQAGIAYATDQIIDLLSNGVNNIHIYTMNKPHVA
                     AAIMNNLSEIIKA"
     gene            complement(6552..>7403)
                     /locus_tag="ACZPD4_04885"
     CDS             complement(6552..>7403)
                     /locus_tag="ACZPD4_04885"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR1378442.1"
                     /translation="IQLTRFHDGFGFLAVGGPVDVNAEEIKTPFYGEGLDGLMSARAN
                     DLCGIVNGLDYTDWDPASDPRIAKTFSVEDFRKNKVLNKKALQEELGLEKDTKTMMIG
                     MISRLTDQKGFDLVDYVMDELCQDAVQIVVLGTGDVKYENMFRHFAWKYSGKVSANIF
                     YSDELSHKIYASCDAFLMPSLFEPCGLSQLMSLRYGTIPIVRETGGLRDTVEAYNEFD
                     KTGTGFSFTNYNAHEMLNTVRYAERIYYDRKRDWNKIVERAMSKDFSWKNSAGQYQEL
                     YKSLIGE"
BASE COUNT         1863 a         1952 c         1244 g         2344 t
ORIGIN      
        1 cctcatcgtc gtcctcatag acggacgggt acaggcggtc cggatcaagg cccaccactt
       61 cggtcaggaa ttcccaggac caggccagcg cttccttttt aaagtagtcg ccaaaggaaa
      121 agtttccgag catttcaaaa aatgtaccgt gacgcgctgt ctttccgaca ttttcaatat
      181 ctcccgtacg gatacatttc tggcaggtag tcactcgctt tctcggcgga atctcctgtc
      241 ctgtaaaata tggttttaac ggcgccatac ccgaattgat aagcagaagg ctcttatcat
      301 tgtgcggcac caatgaaaaa ctcttcatcg caagatgtcc tttactctca aagaactcta
      361 aaaacatttt tctaagttca tttactccat atgccttcat tgcattttcc tcctctttcg
      421 ctccaaaagc ctgttttata atatatcata gagggttata tttgtaaagc ggacgccaaa
      481 aaaaggcgcc cgcttactta tcttatccca tattttatct gcaattttcc gtttacggca
      541 tttccgcttt cttttcttgc agtccggctg tcaaatttgt atttatttta atcaagttcg
      601 atctcttcta attctttcgg cggaattgac ttacttgagt ttttcacgaa agccgcaaga
      661 tctttcttcg ccttcggaag aggaatattc gttcccgctc cggcaataca tcctcccgga
      721 catcccattc cttcgatcag acaaccgttc atcttgcctg cctttgccag agttaatatc
      781 tttttacatt ccttcaatcc ttctgcgtgc tcaatattca cctgtacatc aggataatat
      841 tcgttaatac atttttctat tgcctcggca actccgcctg cacatccgta tcccctgcca
      901 gctccggttg catcatggaa ggaagactct gcttcatact ctgtcagatc gatctctttt
      961 gcatcaaaca ttgcctgcaa ttcctcaaaa gtaacaacaa aatcaacgtc acttcgcacg
     1021 cttcttcttg acgcttccag tttcttcgcg gcacatggtc cgataaagac tacttttgct
     1081 tcagggtgtt tctgcttaat tgtccttgcc gtcgccacca taggagtaag ttcctgggag
     1141 atctggtcga tcagatccgg gaaaaatttt tttgccataa cagaccatga aggacaacag
     1201 gatgtcaaaa gaaacggcag ttcacccgtc acaactttat ccacatagtg atgcgcctct
     1261 gcaatcgcgc cgatgtctgc tccaagcgca acttcataca cttccgaaaa tccgagttca
     1321 cgaagagcgg ctttaatatt tctcggtgta atattatcac cgaactgtcc tacaaatgcc
     1381 ggagcgatct ccgcaattac attttcacct tctgataacg cccgtgcaag ctggaatatc
     1441 tgagacttat ccgaaatcgc gccaaacgga caacttacca tacacatacc gcatgaaaca
     1501 catttttcat tatcaatata tgctcttccc attttgtcgg atttaatcgc attcacaccg
     1561 caggctttct gacatggacg ctcctttttc gcgatcgcat cgtacggaca gactgatttg
     1621 cattttccgc atttgataca tttttcctga tcgatatacg accgcccgtt caccatggaa
     1681 atagcgtctt tcggacagac ttcctgacac gggtgcgcaa gacagccttt acagatattg
     1741 cttacctcgt atttattctc ttcgcagctt tcacatgcgg acggaataac ctgcatcagc
     1801 ggaggctcat aatatttttc agagatattg cttgcttcca cgcccgcagt taaatgaacc
     1861 ggcttatttt ccggacgcag ggaaagtccc atcgccaaac gcagccgttc ccttacgatc
     1921 gcccgtgcgc ggtaaacgct ttctctgtat ttctctgaat cttcatttac gatcgcatac
     1981 ggaatggctt ccatatccgc aagaagcgta tccgcatctg ccttaaatcc aagctgcgcc
     2041 acttccttaa acactcttct ccggattgcc cgtaccggag tatgtaatcc tctcatcatc
     2101 tgcacttacc tcctgatata ctaaattgaa acgacgagca catctgttcc tatttcttta
     2161 aatatatctg tttacaaaat cattgtcaac aaccgaatat tctttctctg acaagtattt
     2221 attctttttt atcggcactt ttttgcagag catctttaaa aatcttttcg gcgtaatgta
     2281 cggactgcat cgcatctttc ccataaaaat ccgcaccgat catatctgca tactcttggt
     2341 tgagcacagc tcccccgacc atcactttac agtccggttt cttttcccga agcatcttta
     2401 tcgtctcttc catactgact accgttgttg tcataagagc gcttaatcca acaagcggaa
     2461 cttgcttttc acatgccgtt tctacaattt tttcaggcga tacgtctttt ccaagatcga
     2521 tcacatcaaa actgtaattt tctaaaagca ctttcacgat attttttccg atatcatgaa
     2581 tatctccttt cacggtcgcc aaaatgactc gtcccttttt ttctctgatc tcaccgcttt
     2641 tttccatctt ttccttcaga acagaaaatg cggattttgc agcttccgca ctcatcagaa
     2701 gctgaggcag aaaaacagtt cctttttcaa agccgtcgcc gacatgattt aatgcaggaa
     2761 tcaattcttc gttgataata tcgagcggag cttctgtcgc aatcatatct gttgtaattt
     2821 tccccgcgtc atcccgcagc cctttttcga ttgcctcttg aagcgtcatt tttgagctgc
     2881 tttttctgct tctgtcattt tcccccttta atttcgtttc ggaaagttca gatgttttca
     2941 ttatatttgt gctcactacc gaattggcat atttttgaat atattgttca caattattat
     3001 ccagattcat aagagcatgg tatgcatacc acgatttcat catgtcttcc gatgatggat
     3061 ttataattcc ggcactcaga ccgtttagca ttgccattgt ataaaaggct gcgtttacga
     3121 tcgggcggca tggaagtcca aaggatatat tagaaactcc gagaactgta ttgactccga
     3181 tctcatctcg aagtctgcga agcgtctcca atgtcacttt tgctccttcc ggctcagaac
     3241 tgatcgtcat tgcgagtgcg tcgattacga tatctttctt tttaatcccg aatttttcag
     3301 ctgtttttat gattttttcc gcaatcctca cacggccgtc cgcatccgcc ggaattccgt
     3361 cttcatccaa agtcagaccg ataacaactc cgccgtattt ttttacaaga ggaaacactt
     3421 tttccattga ctctttcttt ccgcttacgg aattaaccat tgcctttccg ttgtaaatac
     3481 gaagcgcttt ttccatcgct ttttcatcaa ccgtatctat ctgcagcgga atattaacga
     3541 cgctttgcag ttcctgaact acctcttcca taagcgccgg ctcgtcaata tccggcagtc
     3601 cgacattcac atcaagaatg tgagctccct gatcctgctg tgtaatccct tctcttaaaa
     3661 tatagtcaag atcgtgttct ttcagcgctt gtttaaaccg ttttttaccg gtgggattta
     3721 tccgctctcc gataattttt gaaccctcac ccaaaataac gctttgcccg taagaggaaa
     3781 caaccgtaaa ctctttttct gtcaccggaa caagttctgc gttttcacat cttttacgca
     3841 tggcacggat atgttccggc gtcgttccac agcagcctcc gatcacacat gcccccatcc
     3901 ggacgatttg ctccatatat gccgcaaatt catcttctgt cacatcataa tatgttttcc
     3961 cgtctctctg tttcggaagt cctgcatttg gctttacaat aatcggaaca gacgaatatt
     4021 tgatcagttc ttccaacacc ggaagcatct cttttggtcc cattgcgcag tttatcccga
     4081 gagcatcgac gcctaaacct tctaaaagcg caacaacaga tgaaacatcc gctcctgtca
     4141 aaagtttttt cctctcgtca aatattacgg tagcaaatac cggaagagac gtgttttctt
     4201 tcgccgctaa aacagctgct tttaattcgt atgtatcgct cattgtctct atatggatta
     4261 aatctgctcc cgcctgctct cctaagatca ctacttcctt aaaagcttca tatgccgtct
     4321 caaattccag atctcccata ggtttcagca atttccccgt aggtccgaca tcaagagccg
     4381 tatagatttt acgctcgtct cctgtttgta agagcgcctc ccgctctgct tttttcacat
     4441 ggctgaccgc cgcctttata atctcttcta aagagcagga atcatcatga aacttcagcg
     4501 catttgcacc aaatgtattc gtcaaaatga tatcgcttcc ggcttctata tattgcctgt
     4561 gtatcttgta tatttcttcc gaatgtgtca gattccacgt ctccggtaat tctcccggcg
     4621 cgagcccttt ttcctgaagc agagttccca ttcccccatc aaaaaagagg aggtcttttc
     4681 caagtcgttg tctgatcatc tctttctcct ttttttcgtc gttttccttc tctacatatt
     4741 tgtctcatac atctaactgt cagcgtctgt aaatacactc tttcttatcg cagacttcgc
     4801 agcctgcaat cgggcatctc tcttttattc gacttgcccc gatcactgcg gtaacagatt
     4861 tcgtcggtgt catcataaaa ctttccgtca tcgtaagtcc gattgccttt gcacaatcca
     4921 gcatctgcat aaatgatttc tgaaaaccga tatcaaaatc gccgtatccc ggactgaacc
     4981 tcgggcgcag ataaagacct tccttttcaa gttcctcgcc gatttcaaat tgtttctcat
     5041 cacaaaattc ttccagcatt gccgctgcac atgcctgaag cacaacagcg tttgccatat
     5101 ctgtcagaga tgttcttgct atcagacgat ccactcctgt ccctaatgtc gcaccgaaca
     5161 aaacagcctt ttcacagtga tttaagtttc ttgcaaggct tttgctgcta atctgtaatg
     5221 ttccgatcgc aactttttct tcatctatcc gattcatttc aaaaatacga aacaccgagc
     5281 gaagcgccga tactttttcc agctccaaaa aacctttttc aacaagacgg cttgttgcct
     5341 catctaccgg atttttccca taccccaaat agcggacagc ttcctgaaaa catttttcca
     5401 ttctatcacc tacgccttta taatctccga caaattgttc ataattgcag ctgcaacatg
     5461 cggtttattc attgtataaa tatgaatatt gttcacaccg ttagaaagaa gatcaatgat
     5521 ctgatcggtc gcatatgcga ttcccgcctg ttccatcgcc gccgccgagc tgccgaaccg
     5581 atcaaggaga gctaaaaatc ttctcggaaa cactgtccct gacaattcct gacttctttt
     5641 catctgtttc tcactcgtca tcggcataat tcccgggagt accggaacat tgatccctgc
     5701 ttccctgatc ctgtataaaa aattataatg gatatcgtta tcaaaaaaca tctgtgtcgt
     5761 aagaaaatca acgccgctat ctactttttg ttttaaatac ctgatatctt ctgccttatg
     5821 ttcgttttct acatgacctt ccggataaca tgcagctccg atacaaaaat cgccatgctt
     5881 acgaatctcc tctatcaact catatgcata tctaaaacga tttccgttcg ggaacggcat
     5941 cccttccggg atatctcccc gaagcgcaag aatgttttca atcccgtttt ctttcagatt
     6001 ttcaatcaca tctcttactt cctctatcgt tgaagaagca catgtcaagt gtgcaagact
     6061 aaccactccg agatcatcct ttatatgaga tgcgatcttt gccgtattct tactcgtacc
     6121 gccgcccgca ccatatgtca cgctgataaa cgccggattt aacttcgcca tagcatcagt
     6181 tgccgcgatc actttttcaa atcccgcatc ggtcttcggc ggaaacactt caaatgaaat
     6241 atgtattttg tcttctttta atctgtctat aattttcatc tttactctcc tgactgtttt
     6301 tctctgtcgt acggctaagt attagaaatc catttccttc gacatttttt aggtaaaatt
     6361 atcctctaaa acgctttttc tgtcaagtat tgtttcttca aacgaccgaa aataccaaaa
     6421 gcacattttg gatttgcaaa cgagtggcaa gaacttagat aaatttaacc ccctgttcgg
     6481 cgctgctgca aaaaaacaca agacgaaacg aatgtccgtc ttgtgttttt atatgtacct
     6541 cttactatgt cttattcccc gatcaagctc ttgtataatt cctgatattg tccggcagaa
     6601 ttcttccatg aaaaatcttt gctcatcgca cgttctacga tcttattcca gtcacgtttt
     6661 ctgtcataat aaattctttc cgcataacga actgtattaa gcatctcatg tgcattataa
     6721 ttagtaaagc taaatcctgt tcctgtcttg tcaaattcat tatacgcttc aaccgtatct
     6781 ctaagtccgc ctgtctcacg cacaatcgga atcgttccat aacgcaggct catcagctga
     6841 cttaatccgc acggttcaaa cagtgatggc ataagaaatg catcgcagga agcataaatc
     6901 ttatgtgaaa gttcatccga ataaaagatg tttgcggata cttttccgga gtacttccat
     6961 gcaaaatgac ggaacatatt ctcatatttc acatcacctg ttccaagcac aacgatctga
     7021 accgcatcct gacaaagttc atccataacg taatcgacaa gatcaaatcc tttttgatct
     7081 gtaagccgtg agatcattcc gatcatcatc gtttttgtat ctttctctaa accgagttct
     7141 tcctgcagcg ctttcttatt aagaacttta ttcttcctga aatcttcaac cgaaaaggtt
     7201 tttgcaattc taggatctga agccggatcc cagtctgtat aatccaagcc gttgacaata
     7261 ccgcaaagat catttgccct tgccgacatt aatccgtcaa gaccttcacc gtaaaacggt
     7321 gttttgatct cttctgcatt cacatcgact ggaccaccta cggcaaggaa gccgaaacca
     7381 tcgtgaaacc gggtaagctg gat
//
LOCUS       DBMDXF010000089         7295 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2652_length_7295_cov_1.861968, whole genome shotgun sequence.
ACCESSION   DBMDXF010000089 DBMDXF010000000
VERSION     DBMDXF010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2652_length_7295_cov_1.861968"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(36..743)
                     /locus_tag="ACZPD4_04890"
     CDS             complement(36..743)
                     /locus_tag="ACZPD4_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1378443.1"
                     /translation="MKCGIKEVSELTGVSARTLRYYDQIGLLKPSKTSEAGYRYYGER
                     ELEILQQILFYKERGMELKQIKKIIYQKDFDIMAALREHLLELEEKRNHMEALIQTVE
                     QTIRSMKGEYDMSDQEKFQAFKERVVRENEEKHGAEIREKYGDEEMDAANRKVLDMTE
                     EEYERFQNLGEEIRIQLQDAVRGGANPEDECGRRIVALHKEWLGKTWKQYTKEAHAAI
                     GNLYISDERFKMYYDKE"
     gene            complement(1034..1300)
                     /locus_tag="ACZPD4_04895"
     CDS             complement(1034..1300)
                     /locus_tag="ACZPD4_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR1378444.1"
                     /translation="MKILMYCLLALNVGVWLMYGIDKWKAKSGRWRIPEKVLLLAAFA
                     GGSVGALAGMMMFRHKTKKAKFVISVPIFFVIHCVIAGYVLLNY"
     gene            complement(1297..4890)
                     /gene="addA"
                     /locus_tag="ACZPD4_04900"
     CDS             complement(1297..4890)
                     /gene="addA"
                     /locus_tag="ACZPD4_04900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIR1378445.1"
                     /translation="MGVKWTEQQQKVIDLRNRNILVSAAAGSGKTAVLVERIIQKITD
                     EQSPVDVDRLLIVTFTEAAAAEMKDRIREAIEKKLEENPGNVHLERQATLVHSAPITT
                     IHSFCLSVIREHFHVIDLDPGFRIGEDGELKLLKQDVLDELLEECYSKGEEEFLSFTE
                     RFGTGKNDRKIEGIILKLYEYSGSYPRPEQWLDRCAAVYTEEEAAEAMIRRAKEHVLI
                     RIEEPEKLIRRGLKICEEPDGPYMYADMLEEDLRGIEKIRKCSDFAEMYDAADRFVWK
                     TLSRKKDDSVSAEKRESVKALREQAKKAIAGVKETYFYAPESVWKEDMVSAGESVKTL
                     VSLVKLFAEKFAEKKRGRNMIDFNDMERFALDILTEEKNGELVPSAVAGEYAKQFEEI
                     MIDEYQDSNFVQETILTSVSGQAEGKNNIFMVGDVKQSIYSFRLSRPELFMQKYNTYS
                     LEDSDRQRIDLHKNFRSRGEVLDSVNYIFRRIMKKEMGGIEYDDSAALYLGAKFPKTE
                     EIEAKRYISSECERNTDLFKTELLILKDRDKEAENVRRSEARMIARRIKSLMRGGVVS
                     DKKTGEYRPVQYKDIVILTRSVKGWAQDFSAVLAEEGIPAYSVSREGYFETYEVSVLL
                     DYLKILDNGRQDLPLAAVLASPLGNLLTEDMAKIRVAYPDVPFHESARMYASEEAQDP
                     VLKDRLMRFFSQVDHFRKKVSYTPIHDLLTEIIEETGFGIYISAMEGGVQRAANVEML
                     VEKAKAFEGTSYKGLFHFVRYIEQLKKYDVDYGEAGIMDEQADTVRIMSIHKSKGLEF
                     PVVFAAGMGKQFNTQDTKSSMILHPEWGIGIDKIDLKRRTKAPTFLKKLIREEAMYEM
                     WAEEMRILYVALTRAKEKLIMTGVLPDRFIMRSGKDGVITAGAKSYFDWIIPALWSEE
                     EEAALEDAPVCIFQYTGNDIGADILQVKSEQLALDVLKHWDVTKVYDPEIRKCLDEQA
                     DYVYPYYSEGGMKMKFTVSELKKRKALQEEAGEEMYKEPQTVPLLPRFIKEEETLSGA
                     SRGSAYHKFLELLDFAKEYEADDLEAEVERLAEDGRLSREMAECIETEDMMRFLRTKS
                     GVRMLQAARNRKLYKEQPFVISVNASEIYQGDLSGEKILVQGIIDVYFEEEDGLVVLD
                     YKTDQVKSRKELAEKYHAQLEYYAHALSQLTGKTVKEKIIYSFTLKEEIIV"
     gene            complement(4893..>7295)
                     /locus_tag="ACZPD4_04905"
     CDS             complement(4893..>7295)
                     /locus_tag="ACZPD4_04905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1378446.1"
                     /translation="LVRTKGYRYRDIAVIASDMNVYADVLEKACASFDIPVFMDHKKS
                     ILLNSFVEYVRSLLGMIQENFTYESVFRYLRSGYCEFTEDEIDRLENYCLALGIKGYK
                     KWQQAWARKTKEADEEEVEKLNHLRVKFVEKIDPLMFVSRQKKKTVLDITLAVYEFIA
                     GEHLQEKLEQQQKFFAEQGELTLAKEYEQIYRIVMELFDKFSELLGDEPITLKEYCEL
                     LDAGFEEAKVGVIPPSIDQVVIGDVERTRMKDIRALFFVGANDVLLPGNAGTGGILSE
                     RDREKFKEKDISLSPGPKEKIYIQKFYLYTALTKPTELLSLSWSKVSGEGKSLRPAYL
                     IQELRRLFPNLSVVDEENRKLSDREITRRGGIEKVAKGICSRRLGLDNEWKELYTWFQ
                     KDGRNKKEIESILNAGFLHKTDTRLAPELTKELYSDRNRVSVTRLEKFASCAYAHFLS
                     YGLRLSDREEYGFEALDLGNIAHKSLETFSKKADAQKMKWQEIPEALRDEMIDESVEE
                     SVIDYGNTVLFSSARNEYMITRIKRLIRTSVWALTKQIEKGDFLPSGYEMQFGSGKID
                     RIDTCFDNDCVYVKVTDYKTGMKSFDITALYHGLQMQLPVYLNAALDVEQRKHPHKTI
                     VPAGIFYYRIQDPIVSEEKTQDAVERSILKELKQDGLVNGDDMVISHLEKELSGNSLL
                     FPIGRNKDGSLSKTSHALPEELFRLVLSFAKRKEESVKDRMYDGEVSASPYEMGETTG
                     CDYCPYRDICGFDPRLEGCSYRRLERYSSDDAVKKMREALEENVSRDASENEQSGKGR
                     EG"
BASE COUNT         1759 a         2008 c         1182 g         2346 t
ORIGIN      
        1 cccggtcaaa gccaataaga gcaggatagc aggaattatt ctttatcata atacatttta
       61 aacctctcgt cgctgatata gagattgccg attgccgcat gcgcctcttt cgtatactgt
      121 ttccatgtct ttccaagcca ttctttatga agtgcgacga tccgcctgcc gcattcatcc
      181 tccgggtttg ctccgcctct taccgcatcc tgtaattgta tgcggatttc ttcacccaga
      241 ttttgaaatc tctcgtattc ctcctctgtc atatctaaaa ctttcctgtt tgcggcatcc
      301 atctcttcat cgccatattt ttcgcggatt tccgccccgt gtttttcttc attttcccga
      361 actactcttt ctttaaacgc ctgaaatttt tcctgatcac tcatatcata ttctcctttc
      421 attgaacgta tcgtctgttc cactgtttgg atcagagctt ccatatggtt tctcttttcc
      481 tcaagttcca aaaggtgttc ccgcaaagcc gccataatgt caaagtcttt ctgatagatg
      541 atcttcttga tctgctttag ttccattccc cgttccttgt aaaacagaat ctgctgcagg
      601 atctccagtt cccgctctcc atagtaccga tatccggctt cacttgtctt tgatggtttc
      661 agcaatccga tctgatcata ataacgaagt gtgcgcgcac tcactcctgt tagttccgat
      721 acttctttaa tgccgcactt caccgttttc acctcctgtt aaaataccct aagccgatat
      781 tttctttctt ttctatcagc ttagggcaag tatattactt gacgttacgt caaagtcaag
      841 agcatctgac atattttttc gtaacagttc atccatacag atgtacagga agcacaaaac
      901 atgcttgcat atttttgcaa gcggacatct gtatgtgctt agcgcctttt tatgagtaaa
      961 acagtggcgg cagccacaat aagcacgcag tgcggtttta cgaatggcgc gggctgaact
     1021 gttacattgt tctttaataa ttcaaaagca catatccggc tatcacacaa tggatcacaa
     1081 aaaatatcgg tacgctgatg acaaattttg ctttctttgt tttatgcctg aacatcatca
     1141 ttcctgctaa agctccaacc gaaccgcctg caaacgccgc aagcagaaga actttttcgg
     1201 gaatccgcca tcttcccgat tttgctttcc acttatctat accgtacatc agccataccc
     1261 caacatttaa tgcgagcaaa caatacatca aaatcttcat acgatgatct cctcttttaa
     1321 agtaaacgaa taaataatct tctcttttac agtttttccc gtaagctgag acagggcatg
     1381 tgcataatat tcaagctgcg catgatattt ttccgcaagt tcttttctgc ttttcacctg
     1441 atccgtctta taatcaagca cgacaagtcc gtcttcttct tcaaaataaa cgtcgataat
     1501 cccctgcact aatatctttt ccccggacag atctccctga tagatctccg atgcattcac
     1561 gctgatcaca aaaggctgct ctttataaag ttttctgttc cttgccgcct gtaacatccg
     1621 cactccgctc ttagtccgaa gaaaccgcat catatcttct gtctcaatac attctgccat
     1681 ttccctcgat aaacgtccat cttccgcaag cctttccacc tctgcttcca gatcatctgc
     1741 ttcgtattct tttgcaaaat caagcagttc taaaaacttg tgatatgcgc ttccccgtga
     1801 agcgccggaa agagtttctt cctcttttat aaaacgggga agaagaggaa ccgtctgtgg
     1861 ttctttatac atttcctctc ccgcttcttc ttgcagcgcc tttctttttt tcagctccga
     1921 tacggtaaac ttcattttca taccgccttc cgaataatac ggataaacgt aatctgcctg
     1981 ttcatccaga cattttcgta tttccggatc atacactttt gtcacatccc agtgtttcaa
     2041 tacgtcaagc gccaactgtt ctgacttcac ctgtaaaata tctgctccta tgtcatttcc
     2101 tgtatactga aaaatgcaga caggcgcatc ttctaacgcc gcctcctcct cttcagacca
     2161 aagagccggt atgatccagt cgaaatatga ttttgcacct gccgtgataa ctccgtcttt
     2221 gccggagcgc ataatgaaac gatccggcaa aacccctgtc atgatcagtt tttcctttgc
     2281 tcttgtcaac gctacataca gaatgcgcat ctcctctgcc cacatttcat acattgcctc
     2341 ttcccgaatc agctttttca aaaatgtagg cgcttttgtc ctccgtttca gatcgatctt
     2401 atcaatccca atgccccatt caggatgaag gatcatgctg cttttcgtat cctgtgtatt
     2461 aaattgtttt cccatacctg ccgcaaaaac aaccggaaat tccaaacctt tactcttatg
     2521 aatactcatg atccttaccg tatccgcctg ttcatccatg atcccggctt ctccgtaatc
     2581 aacgtcatat tttttcagtt gttcgatata ccgcacaaaa tgaaacaatc ctttgtagct
     2641 tgttccttca aacgcctttg ctttttccac cagcatctct acgtttgcgg ctctttgcac
     2701 tccgccttcc atcgccgaaa tgtatatacc gaaacctgtt tcttctataa tctccgtcaa
     2761 cagatcatga atcggagtat acgacacttt ttttctgaaa tgatccacct gactgaaaaa
     2821 tctcatcaga cgatctttca agaccggatc ctgtgcttct tccgacgcgt acatccgcgc
     2881 gctttcatga aacggtacgt cgggatatgc gactctgatc tttgccatat cttctgtcag
     2941 aagatttccc aaaggcgacg ccagaaccgc cgcaagcggg agatcctgcc ttccgttatc
     3001 gagtatttta agatagtcaa gcaatacgct cacttcataa gtttcaaaat acccttccct
     3061 gcttacagaa tatgcgggaa tcccttcttc tgcaaggact gccgaaaaat cttgtgccca
     3121 tcctttcaca cttcttgtca aaatcacaat atctttatac tgcaccggac gatattcccc
     3181 tgttttttta tccgacacaa ccccgccgcg catcaaactt ttaattctgc gcgcgatcat
     3241 cctcgcttct gaccgacgta cgttttccgc ttctttatcg cggtctttta agatcaaaag
     3301 ctccgtcttg aaaagatctg tgtttctttc acattctgac gatatgtatc gttttgcctc
     3361 aatttcttct gttttcggaa acttcgcccc aagataaagc gccgcgctgt catcatattc
     3421 gattccgccc atttcttttt tcataatacg tcggaagata taattgacac tgtccagaac
     3481 ttctcctctg cttctgaagt ttttatgcag atcgatcctc tgtctgtcac tgtcttctaa
     3541 actgtatgtg ttatattttt gcataaacag ttcaggtctt gaaagacgga agctgtaaat
     3601 gctctgcttt acatcgccta ccataaaaat attatttttt ccttctgcct gtcccgatac
     3661 gcttgtcagg atcgtctcct gtacaaaatt actgtcctga tattcgtcga tcataatttc
     3721 ttcaaactgt tttgcatatt ctccggctac ggcagacgga acaagttctc cgtttttttc
     3781 ttcagtcagt atatcaagcg caaaccgttc catatcgtta aaatctatca tatttctgcc
     3841 gcgcttcttt tccgcaaact tttctgcgaa cagctttacc aaagatacga gcgttttcac
     3901 agactctccc gccgaaacca tgtcctcttt ccatacgctt tccggcgcgt aaaaataagt
     3961 ttccttcacc ccggcaatcg ccttttttgc ctgttctctc aacgccttta cggactctct
     4021 tttttcagcc gatacactgt catctttttt tctcgaaagc gttttccata caaaccgatc
     4081 agccgcgtca tacatttctg caaaatcact gcattttctt atcttctcta ttccacgaag
     4141 atcctcttcc agcatatccg catacatata cggtccatcc ggctcctcac atattttcaa
     4201 tcctctgcgg atcagttttt ccggttcttc gatccgaata agaacgtgtt cttttgcccg
     4261 ccgtatcatc gcttccgccg cctcttcttc tgtatatacc gcagcacaac ggtcaagcca
     4321 ctgttccggt cttggataac tgccggaata ttcgtataat tttaaaatga tcccttcgat
     4381 cttccggtca tttttccctg ttccaaaccg ctctgtaaag gaaaggaact cttcctctcc
     4441 tttggaataa cactcttcaa gcaattcatc gagaacgtct tgttttaaaa gttttaattc
     4501 tccgtcttct ccgatccgaa atcccggatc aagatcgatc acatgaaaat gttcccttat
     4561 cacagacaga caaaaactgt gtatcgtcgt gatcggcgca ctgtgaacta acgtagcctg
     4621 cctctccaga tgtacgtttc ccggattttc ttccagcttt ttctcgatcg cttctctgat
     4681 cctgtccttc atctctgctg ccgccgcttc tgtaaatgtc acaatcaaaa gtctgtccac
     4741 atcgacagga ctttgctcat ctgtgatctt ttgaataata cgttctacga gaacggctgt
     4801 ctttcccgat cctgccgccg cagatacaag aatattacga tttcgcagat ctatgacctt
     4861 ttgctgctgt tctgtccatt tcacgcccat tcttatcctt ccctgccttt cccgctttgc
     4921 tcgttttccg aagcgtcccg gctgacattt tcttccaaag cctcccgcat ctttttcacc
     4981 gcatcgtctg aagaatatct ctccagcctg cggtaactac atccttcaag acgcggatcg
     5041 aacccacata tatctctgta cggacagtaa tcacatcctg tagtttctcc catctcatac
     5101 ggagaggcgg aaacttctcc gtcatacatt cggtctttga ccgactcttc ttttcgtttc
     5161 gcaaaagaaa gtactagccg aaacagttct tccggaagcg cgtgagacgt tttactcaat
     5221 gaaccgtctt tatttcttcc tattggaaaa aggagcgaat tccccgaaag ttctttctcc
     5281 aaatgcgaaa tcaccatatc atccccgtta actagcccat cctgctttaa ttcttttaaa
     5341 atacttctct ccacggcatc ctgagttttt tcttccgaaa cgatcggatc ttgtatccga
     5401 taataaaata ttcctgccgg gacgatcgtt ttatgaggat gttttctttg ctctacatcc
     5461 aaagccgcgt tcagatatac cggaagctgc atttgcagac catgatacaa cgccgtaata
     5521 tcaaaacttt tcattcccgt tttataatct gtcactttga cataaacaca gtcattgtca
     5581 aaacatgtat cgattcggtc aatcttcccg cttccgaact gcatctcgta cccgcttgga
     5641 agaaagtctc ctttttctat ctgctttgtc agcgcccaca cagaagttcg gataagacgt
     5701 ttgatccgag tgatcatata ttcatttcgc gccgaactga aaagaactgt attcccataa
     5761 tctattacgc tttcttccac actctcatcg atcatctcgt cacgaagagc ttccggtatt
     5821 tcctgccatt tcattttctg cgcatccgcc tttttggaaa acgtctccaa agacttgtgc
     5881 gcgatatttc cgagatcaag cgcctcaaac ccatattctt cccggtctga aagccgcagt
     5941 ccgtaactta aaaaatgagc atatgcacaa gacgcaaatt tttccagtct tgtcacactg
     6001 acgcgatttc gatcactata aagttctttt gtcagttccg gtgcaagcct cgtatctgtt
     6061 ttatgcagaa aacctgcatt caaaatactt tcgatctctt ttttatttct tccgtctttt
     6121 tgaaaccatg tatacaactc tttccactca ttatccagcc cgagacggcg gctgcaaatt
     6181 ccttttgcca ctttctcgat cccccctctt cgggtgatct ctctgtcgct aagctttctg
     6241 ttttcttcat cgacaacgga cagattcgga aacagcctgc gaagctcctg tatcaaatac
     6301 gccggacgca gagattttcc ctcccccgac acctttgacc atgacaggct tagaagttcc
     6361 gtcggttttg tcagcgccgt atataaataa aatttctgta tatagatttt ttccttcggt
     6421 ccgggcgaca acgagatatc tttctctttg aatttttctc tgtcccgctc agacaaaatg
     6481 ccgcccgttc ccgcattgcc cggcaaaagc acatcgttcg ctccgacaaa aaacaaggcg
     6541 cggatatctt tcattcttgt acgctctacg tctccgatca caacctggtc aatgctcgga
     6601 ggaatcactc ccactttcgc ctcttcaaat ccggcgtcaa gaagctcgca gtattctttc
     6661 agcgtaatcg gctcgtcgcc taacagttcc gaaaacttgt caaacaactc catgacgatc
     6721 cggtagatct gctcatattc ttttgcaagt gtaagttctc cctgttctgc gaaaaatttc
     6781 tgctgttgtt ccaatttctc ctgaagatgc tctcctgcga taaattcata taccgcaagc
     6841 gtaatgtcaa gcaccgtctt ttttttctgt cttgaaacga acataagcgg atcgatcttt
     6901 tcgacaaatt tcacgcgcag atgatttaac ttttccactt cttcctcatc cgcctctttc
     6961 gttttcctcg cccatgcctg ctgccatttt ttatatccct ttatccccag cgcaagacag
     7021 tagttttcca atcggtcgat ctcatcttcc gtaaattcac aatatccgga tcttaaataa
     7081 cgaaatacgc tttcataagt aaaattttcc tgtatcatcc ccagaaggct gcggacatac
     7141 tctacaaaag aattcagaag aatacttttc ttatgatcca taaacacagg tatgtcaaaa
     7201 gaagcacatg ctttttccaa cacatccgca tacacattca tatctgacgc gatcacggca
     7261 atgtctctgt atcggtatcc ttttgtcctc actaa
//
LOCUS       DBMDXF010000090         7266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2662_length_7266_cov_2.139288, whole genome shotgun sequence.
ACCESSION   DBMDXF010000090 DBMDXF010000000
VERSION     DBMDXF010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_7266_cov_2.139288"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(23..1150)
                     /locus_tag="ACZPD4_04910"
     CDS             complement(23..1150)
                     /locus_tag="ACZPD4_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HIR1378447.1"
                     /translation="MENTGDLEDYQNAENQFTDLSAQLEKPMANRIISFSKDSGVITG
                     NDRTLTLSKSSDAKDFEIRYTTDGSIPRHTSDRYEHPLQLDNKENTVVRAALFYNEQR
                     VSPVYTKKYIAKSIKIQDVTVSHTEDWGNNYVKAGMIDQNTSTRWASKNVDASKPLEI
                     TLNFSEKETLDQMNFDLFVSKNNGIGAFEIQALSDGTYRTVYEGTKMGNIDDKVGDID
                     GGSGGYHAYYFAEFPETTTESVKVILKPGFLGEPSLYEIAPLYTGVQADGAGDTSELE
                     KMIRSAEEADRTADHYVNAPQTLKTAFEESISDGKEQLKASQDIIDSRTEFLSDRYHR
                     LGFGTTDKSKLNSLITKAEQELNGNYTNDSLYQLKKVLSDA"
     gene            complement(1199..4129)
                     /locus_tag="ACZPD4_04915"
     CDS             complement(1199..4129)
                     /locus_tag="ACZPD4_04915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HIR1378448.1"
                     /translation="MKTRMKKMLAGMCAVLILPIGSAQTVLAEEPEQNFPISVEKAVN
                     QTLKLWYGSPAKINTAESSGGEWMQQSLPLGNGNLGNLIFGGISKERIHFNEKTLWTG
                     GPSSSRPNYQFGNKATAYTATEIENYRKLLDDKSSNVFNDDQSLGGYGMGAKIRFPGE
                     DNLNKGSYQDFGDIWLDFSAMGITDDNVQNYRRELDLQTGIASTEFSYKNVSYKREHF
                     VSSPDQVMVTNLSASEKGKLNFSAKMELNNDNLEGKLTFDVRNQTCTIEGKVKDNDLK
                     FRTTMKLLLTGGEITADEKNQVYRIKNADQVTIIMAAETDYKNDYPTYRDKEKNLSNV
                     IDTRINDSSKKSYDELKQTHIEDHQSLFDRVSLDLGEFQTSVPTDQLIDEYRNGSYSH
                     YLETLAFQYGRYLTIAGSRGTLPSNLVGLWTVGPSAWTGDYHFNVNVQMNYWPVYSTN
                     LAECGTTFVDYMDKLREPGRLTAERVHGIKGAVDNHTGFTVHTENNPFGMTAPTNAQE
                     YGWNPTGAAWAVQNLWWHYEFTQDEAYLKNTIYPIMKEAAQFWDSYLWTSEYQKINDE
                     TSPYHGENRLVAAPSFSEEQGPTAIGTTYDQSLIWELYNECIQAGKIVGEDEAVLQSW
                     EEKMQKLDPIEINATNGIKEWYEETRVGQETGHNKSYAKAGDLAEIAVPNSGWNIGHN
                     GEQRHASHLVGLFPGTLINKENPTYMNAAIQSLTERGEYSTGWSKANKINLWARAENG
                     EKAYKLLNNLIGGNSSGLQHNLFDSHGSGGGDTMMNGTPVWQIDGNFGLTSGVAEMLV
                     QSQSGYTQFLPAIPDAWEKGEVRGLKARGNFTIGEKWANGIAEAFTVRYDGNKDSAVF
                     TGSYKNITSAKIYEDGKEVQVTKDTDKDRISFEADAGKTYTIDISETNINELKEQAST
                     FLNQLHPDLINAKNELQTAIDLSSKELGTVLTKVKLMDQLYRSYLNKAENIYYLTDKD
                     NLTY"
     gene            complement(4375..5031)
                     /locus_tag="ACZPD4_04920"
     CDS             complement(4375..5031)
                     /locus_tag="ACZPD4_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIR1378449.1"
                     /translation="MKHSLQMSETLRLGIILALSGGFMDAYSYLERGNVFANAQTGNL
                     LLFGVNLAEGNYFAMLSYLLPVLAFTLGILLADTVRCLNIRLHWRQLSVLLEAVILIC
                     VAFFPHSLNPLANSLTSFACGIQVESFRKIRNRGAATTMCIGNLRNGTENLHRFLRTH
                     DRKAFYNALLFYGIILCFIIGAVLGNFVIKRFHEKSILVCSALLFIAFFMMFVNKEQK
                     "
     gene            complement(5102..6451)
                     /locus_tag="ACZPD4_04925"
     CDS             complement(5102..6451)
                     /locus_tag="ACZPD4_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378450.1"
                     /translation="MEKKNSFMQTKPVFSLLLSMSVPMMLSMLIQSLYNIVDSIYVSR
                     LGTDALTAVSLAYPLQNAILSAAVGIGVGISSAISIHLGAGNREKADRAATLGIALTL
                     IHCIFFIIAGLTISEPFLKLFTDNPKILNDASIYTEIVLCLSFGSLFQIAFEKIFQGI
                     GEMKITMYLLIAGCVVNIILDPILIFGLLGFPALGVAGAAIATVIGQISALLLYIIVY
                     RRNSYAVHISFKHLVFDKAIIRQIYSVGIPSTVMMLLPSILISVLNSILTAFSDVYVA
                     VLGVYFKLQTFIYMPANGIVQGMRPIIGYNYGAGESSRVKSTIRYSLLTAAGLMLTGT
                     ILALALPKQILSLFDADAALMSAGVTALRIISLGFLVSSVGLIYSGVFEAIGNGRNSL
                     IISLLRQFVITIPLSFILTRFFGPTGVWISFPVGELCASAAAFCLLKKYQNGAFSPL"
     gene            complement(6517..7077)
                     /locus_tag="ACZPD4_04930"
     CDS             complement(6517..7077)
                     /locus_tag="ACZPD4_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR1378451.1"
                     /translation="MGLTELFILAVGLSMDAFAVAVCKGLAMPKVTWKKITAVGLWFG
                     GFQALMPAAGYFLGIRFKDGITAIDHWIAFILLSIIGINMIREAFSGDEESSNDLLDC
                     KTMFPLAVATSIDALAVGITFAFLNVHIIPAVSFIGVITFIIVAAGVKIGNIFGTRYK
                     SKAELAGGIILILLGVKILLEHLGIF"
BASE COUNT         1896 a         1485 c         1654 g         2231 t
ORIGIN      
        1 ttttatccgg atgctaattc tactatgcgt ctgagagaac ttttttcagt tggtataaag
       61 aatcgtttgt atagtttccg tttagttcct gttctgcttt tgtgatcagc gagtttaatt
      121 ttgacttatc tgtcgttccg aatccgagac ggtgatatct gtcagataaa aattcggtac
      181 gggaatcaat aatatcctgc gatgctttca gttgttcttt cccgtctgaa atactttctt
      241 caaacgcggt ttttaatgtt tgcggcgcgt ttacataatg gtcggcagtt ctgtcggctt
      301 cttctgccga acgtatcatt ttttctaatt ctgacgtatc tccggcgccg tctgcctgta
      361 cgcctgtgta aagaggcgct atttcatata gagacggttc gcctaagaat cccggtttta
      421 atattacctt tacggattct gtcgttgttt cgggaaattc ggcgaagtaa taagcgtgat
      481 atccgccgct tccgccgtcg atatcgccga ctttgtcgtc gatatttccc atttttgttc
      541 cttcatacac agttcgatat gttccgtcag aaagcgcctg aatttcaaaa gcgccgattc
      601 cgttattttt ggatacgaac agatcgaaat tcatttgatc gagcgtttct ttttcggaga
      661 aattcaatgt gatttccaac ggtttagaag cgtctacgtt tttggatgcc caccgtgtcg
      721 aagtgttttg atcgatcata ccggctttta catagttatt gccccagtcc tctgtatgag
      781 atacggttac gtcttggatc tttatgcttt ttgctatgta tttctttgtg tatacagggc
      841 tgactctctg ttcgttataa aacagcgccg ctctgacaac ggtattttct ttattgtcta
      901 attgcagagg gtgttcatac cggtcagatg tatgtctcgg aatacttccg tctgttgtat
      961 aacggatctc aaagtctttg gcgtctgaac tcttactcaa agtaagtgtc cggtcgtttc
     1021 ctgtgatgac accggaatct ttgctgaatg aaatgatccg gtttgccatc ggtttttcta
     1081 attgtgcgct aagatctgta aattggttct cggcgttttg ataatcctct aaatctccgg
     1141 tgttttccaa cagagtcttg cgcagtttgc gaagctgatt gtaaagcgtg tcgatctgtt
     1201 aataggttaa gttatctttg tcggtcagat aatatatgtt ttctgcctta ttcagatagg
     1261 agcgatataa ctgatccatt agtttgactt ttgtcagaac tgtacctaat tcttttgaag
     1321 aaagatcgat tgctgtctgc agttcgtttt tggcattgat gagatcaggg tgaagctgat
     1381 ttaaaaatgt agacgcctgc tcttttagtt cgtttatatt tgtttcggat atatcgatcg
     1441 tatatgtctt gccggcgtct gcctcaaagg aaatacgatc tttatcggta tcttttgtca
     1501 cctgaacttc tttgccgtct tcgtagatct ttgcagaagt aatattttta tatgatcctg
     1561 tgaaaacagc gctgtcttta ttgccgtcat aacgtacggt aaaggcttcc gcaatgccgt
     1621 tcgcccattt ttcgccgatc gtgaagttac ctctggcctt tagacctctg acctctcctt
     1681 tttcccatgc gtctggaatt gcggggagga attgtgtgta gcctgactgc gattgaacaa
     1741 gcatttctgc tacgccggaa gtaagaccga aattgccgtc gatctgccat accggagttc
     1801 cgttcatcat tgtgtctcct ccgccggatc cgtgagaatc gaagagatta tgctggagac
     1861 cggaagaatt tcctccgatc agattgttca aaagcttata tgccttttct ccgttttcgg
     1921 ctctcgccca gagattgatc ttgtttgctt tcgaccagcc tgtagaatat tctcctcgtt
     1981 cggtcaatga ttgaatagcg gcgttcatgt aagtcggatt ctctttgttg atcaatgttc
     2041 cgggaaagag tccgaccaga tgggaggcgt gtctttgctc tccgttatgt ccgatattcc
     2101 atccgctgtt aggtactgcg atctctgcca gatctcctgc ttttgcataa gatttgttat
     2161 gtccggtttc ctgacctaca cgggtttctt cgtaccattc tttaattccg tttgtagcgt
     2221 tgatttcaat cggatcaagt ttttgcatct tttcttccca agattgcaga acagcttcat
     2281 cttctccgac gattttgcct gcctggatac attcgttata taattcccat ataagagatt
     2341 ggtcgtaagt tgttccgatt gcggtcggtc cttgttcctc tgaaaaacta ggcgctgcta
     2401 caagtctgtt ttcaccgtga taaggcgaag tttcgtcgtt gattttctgg tattctgacg
     2461 tccagagata agagtcccaa aattgtgccg cctctttcat gatcggataa atagtatttt
     2521 ttaaatatgc ttcatcctgc gtaaactcgt aatgccacca aaggttttgg acagcccatg
     2581 ccgctcctgt cggattccag ccgtattcct gcgcgttagt aggagcggtc atgccgaaag
     2641 gattgttttc cgtatgtacg gtaaatccgg tgtggttatc gacagcccct ttaataccgt
     2701 gaactctttc tgccgtcagg cgtcccggtt ctcttaattt atccatgtaa tctacaaaag
     2761 ttgttccgca ttcggcaagg ttcgttgaat ataccggcca atagttcatc tgtacattga
     2821 cgttaaaatg ataatctccc gtccacgcgg aaggacctac tgtccataag ccgacaaggt
     2881 tgcttgggag cgtacctctg gaaccggcta ttgtcagata acgtccgtat tggaatgcaa
     2941 gagtttccag gtagtgcgag tagctgccgt ttcgatattc gtctatcaac tgatctgtcg
     3001 gaacggatgt ctgaaactct cctaagtcta aacttactct gtcgaaaaga ctttggtggt
     3061 cttctatatg cgtctgcttt aattcatcgt aagatttttt cgagctgtca ttgatccggg
     3121 tgtcgataac attcgacaga tttttctctt tgtcccggta agtaggataa tcgtttttgt
     3181 aatctgtctc ggcagccatt atgattgtga cctgatctgc atttttaatc cggtaaacct
     3241 gatttttttc atctgccgtt atctcgccgc cggttagaag gagtttcata gttgtacgga
     3301 attttaaatc attatctttt acttttcctt cgatagtgca agtttgattc cggacatcga
     3361 atgtaagctt tccttctaag ttatcgttgt ttagttccat ttttgcgctg aaattaagtt
     3421 ttcctttttc agaagcggag aggtttgtta ccattacctg atccggactg cttacaaaat
     3481 gctctctttt gtaagaaaca tttttataag aaaattctgt tgatgcaatt ccggtttgta
     3541 aatccagttc tctccgataa ttctgcacat tatcgtcagt gattcccatt gcggaaaaat
     3601 caagccaaat atcgccgaaa tcttgataac tgcctttgtt tagattgtct tcgcccggaa
     3661 atctgatttt tgcccccata ccgtaaccgc cgagactttg gtcatcgtta aatacattag
     3721 aagatttgtc atcaagcagt tttcgataat tttcaatctc tgtggcagta tatgccgttg
     3781 ctttatttcc gaactggtag ttgggacggc tcgatgaagg accgcctgtc caaagtgttt
     3841 tttcgttgaa atggattctt tcttttgaga ttccgccgaa aatcaaattt cccagattcc
     3901 cgtttccgag aggcagagat tgctgcatcc attcgccgcc gctggattcg gcagtattaa
     3961 ttttggcagg agaaccgtac catagtttta atgtctgatt taccgctttt tctacggata
     4021 tcgggaagtt ttgttccggc tcttcggcaa gaacagtttg cgcggaaccg atgggaagta
     4081 tgagaaccgc acacatcccc gcaagcattt ttttcattct tgttttcatc attgtctcct
     4141 ctaatttctg tagattcata aataagtata aaattctatt atgaatatat cgtttggaga
     4201 ggagattgtc aatttaaaac aggatattaa aagctgtttt ttgacacatt ccggattgaa
     4261 atggacaatt aacaaaaaat tctgaaaatt caaaaactga gtttgtggtc attgcttttt
     4321 tgagcattga taaatcgagt tataactgtg gaaaccggtg tcggtctgca gaatttattt
     4381 ttgctctttg ttgacaaaca tcatgaaaaa tgcaataaac aaaagcgctg aacatacgag
     4441 gatcgatttc tcatgaaagc gtttgatgac gaagtttccg aggactgctc cgattataaa
     4501 gcagaggata atgccgtaga aaagaagggc gttgtagaat gctttacggt catgggttcg
     4561 cagaaagcgg tgcagatttt cggttccgtt tcttaagttt ccgatacaca tagttgtagc
     4621 cgctcctctg ttgcggatct tgcgaaagct ttcgacctga atgccgcagg caaaagaggt
     4681 gagtgagttt gcgaggggat ttaaagagtg agggaaaaag gctacacata ttaaaataac
     4741 agcttccaac agaacggaaa gctgacgcca gtggagtcgt atgttgaggc agcgcacagt
     4801 atcggcaagt aagatgccga gagtaaacgc gagaacaggg aggagatagc tgagcatggc
     4861 aaaatagttc ccttccgcga gatttacacc gaataggagc agatttccgg tttgggcgtt
     4921 tgcgaatacg tttccccgtt caagataaga gtaggcgtcc ataaatccgc cggataaggc
     4981 gaggatgatt cctaagcgga gtgtttcaga catttgcaaa gagtgtttca tagggaaatc
     5041 tcctttcggt aattaaaatg gttttgctga ggatgtcgta ttatatagaa ggagcatccc
     5101 gtcaaagagg cgagaatgct ccgttttgat atttttttaa taggcagaac gccgcggcgg
     5161 aagcgcagag ctcacctaca gggaatgaga tccatacgcc cgtaggtccg aaaaagcggg
     5221 tgaggatgaa acttaacggg attgttatca caaattggcg gagcaaagag atgatcagag
     5281 aattccgccc gtttcctatc gcctcaaata cgccggaata gatcagaccg acagaagaaa
     5341 caagaaatcc gaggctgatg atgcgaagag cagtgactcc ggcgctcatt aaagcggcgt
     5401 ctgcatcgaa cagagagaga atctgtttcg gaagggcaag agcgaggatc gttcctgtca
     5461 acatcaatcc tgccgcagtg agcaggctgt atcgtattgt tgatttgacg cgggaacttt
     5521 caccggcgcc gtaattgtac ccgatgatcg gacgcattcc ctgtacgatt ccgtttgcag
     5581 gcatatagat gaaagtctga agtttgaaat aaacgcctaa aactgcgaca taaacgtctg
     5641 aaaatgcagt aagtatactg tttagtacac tgatcaatat agacggcagg agcatcataa
     5701 cagtagaggg gattccgaca ctgtaaattt gtctgatgat cgctttatca aagacaagat
     5761 gcttgaagct gatgtgtacc gcgtaagagt ttcttctata tacgatgatg tataagagta
     5821 aggcgctgat ctgtccgatc acggtggcga tggcagcgcc tgcaacgcca agcgccggga
     5881 aaccgagcaa tccgaagatc aggatcggat cgagtataat attgacgaca cagccggcga
     5941 tgagaaggta cattgtaatc ttcatttcac cgattccctg gaagatcttt tcaaatgcaa
     6001 tttgaaaaag agagccgaaa gaaaggcaaa gtacgatttc cgtatatatg gatgcgtcgt
     6061 ttaatatttt cgggttgtct gtaaaaagtt ttaaaaacgg ttcggagatc gtcagacctg
     6121 caatgataaa aaagatgcag tggatcaatg taagggcgat tcccagagtc gccgcccggt
     6181 cggctttttc ccgatttccc gctcctaaat gaatggagat agcggagctg atcccgacgc
     6241 cgattccgac cgctgcggat aagatcgcgt tttgaagcgg ataggcaagc gaaacggcag
     6301 tcagagcatc tgtgccaagt ctggaaacgt agatactgtc aacgatgtta taaagcgatt
     6361 gaataagcat ggacagcatc ataggtacag acatagaaag cagaagagaa aaaacaggtt
     6421 tcgtctgcat gaatgaattt ttcttttcca taataataaa agtctccctg atgtaatact
     6481 tttgccgata aaaatatcgg atcaaaagtt tttcatttaa aatatgccga gatgttcgag
     6541 caggatctta acgccgagaa gaatcaggat gatacctccg gcaagctctg ctttcgattt
     6601 gtatcttgtc ccgaatatgt ttccgatttt tacacctgcg gcaacgatga taaacgttat
     6661 tacgccgata aatgaaacgg caggaataat atgtacattt aaaaaggcga aggtgatgcc
     6721 tacggccaga gcgtcaatac ttgtcgcaac agccagaggg aacatcgttt tgcaatctaa
     6781 aaggtcgttt gaagattctt cgtcgccgga gaaagcttcg cggatcatat tgattccgat
     6841 gatggaaagc agtataaacg caatccagtg atctatagcg gtaatgccgt ctttaaaccg
     6901 gatgccgaga aagtagcctg ctgcaggcat aagagcctga aatccgccga accaaagacc
     6961 gacggcggta atctttttcc aggtgacttt cggcatagca agtcctttac agacggcaac
     7021 ggcaaaagca tccatggaaa gtccgactgc caggatgaaa agttctgtaa gtcccatata
     7081 aaaaactcct taggaattat aaatcttaaa aataatagca ctaaaaatac tgtttttcaa
     7141 gagaaaggaa cgggggtttt gggacgagaa ataaaacaaa ttcacaggat tgacatattt
     7201 gtatggtaat ctgaaaataa aagagaaatt atcggaatat ttttgccggt acatttttat
     7261 atgaga
//
LOCUS       DBMDXF010000091         7160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2705_length_7160_cov_1.703641, whole genome shotgun sequence.
ACCESSION   DBMDXF010000091 DBMDXF010000000
VERSION     DBMDXF010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2705_length_7160_cov_1.703641"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="ACZPD4_04935"
     CDS             <1..654
                     /locus_tag="ACZPD4_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378452.1"
                     /translation="TIFIILIAAVGYIIFQGIKNFQLHNMNVGLKNKDSVLVEKTADM
                     WITRKLLGEYVCDLYKLRVLYVTKDEEKFEKMLIHMLDTTYPRPDDKQSFLETYFHTF
                     LIKGNRKYADWILKEIKKTGDEAFIHYNENAYAVMLDGRTDLIEEMDEDINSKRYYGF
                     ALGVILVMISKQYSALGDDKNALIYMQSAKACLHPKAVYMPVVDRYLREAEAQEQDS"
     gene            706..1134
                     /gene="agaF"
                     /locus_tag="ACZPD4_04940"
     CDS             706..1134
                     /gene="agaF"
                     /locus_tag="ACZPD4_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HIR1378453.1"
                     /translation="MIGLIVTGHANFGSGMTSSVNLIAGEQEAYRYVDFLPTYGTEEL
                     TEEIAKAMDELKDCEGIIIFTDLMGGSPFNVAASLGHGKENVRIVAGTNLPMLVEIVM
                     SRKFMDDLDGLVESVLETGKEQVTKYEFKQVVQEESEDGI"
     gene            1249..2430
                     /locus_tag="ACZPD4_04945"
     CDS             1249..2430
                     /locus_tag="ACZPD4_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1378454.1"
                     /translation="MSTIFGITEDKMKETSSTFTLTEIYQQPATWEKTCRQIEENKEE
                     LQKFIDQVIKCDDFDVILTGAGTSEFVGNALFAHLSGLLNHKAKSYGTTDIVATPEAY
                     LSRTKPTLLISFGRSGNSPESVGAVDAAESVCDNVYHLFVTCNKNGALSKRAAETHNC
                     YAINLTDETHDQSFAMTSSYSNMYLATYLCFHLNELEETVEKVRKIAAAGQNFLDNHY
                     SVAQQIVDEYNFERIVYLGSNTLKGTSQESALKMLELTAGRVVTMYDTPMGFRHGPKS
                     IVDDTTLTVVYLSDEAYTRQYEVDLLKEMSGQRKGNKIVVVMSSADEAVSALADYTVV
                     YDMKEENENILLGLDYILFAQTLAVLKSLSLDITPDNPCPTGEVNRVVKGVTLYPYTR
                     K"
     gene            2595..2882
                     /gene="yajC"
                     /locus_tag="ACZPD4_04950"
     CDS             2595..2882
                     /gene="yajC"
                     /locus_tag="ACZPD4_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1378455.1"
                     /translation="MAINSEVIIWTCVTVAVLVGICAIVLIAMSSRNMKKNREAMRDL
                     QGAIKVGARIMFAGGIYGKIVKIKNDVIDVEINKSTVIQISRYSIQNVITD"
     gene            complement(2901..4097)
                     /locus_tag="ACZPD4_04955"
     CDS             complement(2901..4097)
                     /locus_tag="ACZPD4_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378456.1"
                     /translation="MTNQQITNKFEPFGAFYDEPLDTQKHNLNQWEFSASSKKLDTLF
                     LFPCEVYIECPAGLGLLLIATDPDMTELKTFPLRHNLKLAPNTAFNVIPLASSLTWNI
                     LIGKNCCKEQQLTDFTKPLSTPYTYQPVSVPFHLRRILDCWFTKQSKACHIVQPAHKS
                     YELIYVYEGSLDITLSSGTNTLQPHDLIIYRSDKADLSVQNGCSYLTVVFEVNHRRSL
                     HILNHTFHCTSEMQQILWKLLIESEEHSYYTHTLMVCYLQEVLLLIMQFYETMNHKTL
                     LTDSKSAQNDLLSEILAYMNKRLTEPLTIEDICHEFFISRSSLQALFKTHLNTSPKNY
                     LLNIKLQKSKELIRENQYTISEIAYRLGFSSIHYFSRLFKKYFNTTPSDYARKAAENQ
                     NRQNKP"
     gene            4212..5360
                     /gene="nagA"
                     /locus_tag="ACZPD4_04960"
     CDS             4212..5360
                     /gene="nagA"
                     /locus_tag="ACZPD4_04960"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1378457.1"
                     /translation="MIIQSKKVWFADQFVEAQIEVKDGKIVDIYEYGTHPVDCDYGDN
                     RILPGFIDVHCHGAYGFDTNDAKEDGLRYWVRNIVDEGVTALLATTITQSEEVLTNAL
                     KNVAKVVEDGYEGAEILGVHFEGPYLDMKYKGAQPEQYIVKPTVEQFKKYQEAANGLI
                     KYITMATETDDDFALTRYCAENGVVVSIGHSAATSKEAVQAFAHGARSMTHVYNGMTP
                     FNHRANGLVGAAYRMKSMYGEIICDGNHSTPLALHQFFEAKGPHYGIMVSDALMAKGS
                     PAGSKFIFGGNEIEIYEDGSAHLTSSKGLAGSTLHINEGLRILIEEALVPVDTAINSC
                     TKNPAACLKIDDRKGSIKVGLDADLVVLDRDYQVLQTYCMGKAMLDKN"
     gene            5389..5925
                     /locus_tag="ACZPD4_04965"
     CDS             5389..5925
                     /locus_tag="ACZPD4_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378458.1"
                     /translation="MAKKKSKPVEKIRLSGRNIYTDKKGRVIFYDMITKKGYLVDKKH
                     ENSALFFKNRFVVLLFAAILFGATFLTWLQAAVAWAVMMAIAEFAFRRGFLKKLEVVN
                     DVDFERRVSALQYIVENKAKGKVIVLIVLYLLLAVLVILNAYVEKYSVGLCVLSGGIA
                     LVGVYFAILHIVALTKMK"
     gene            complement(6017..6868)
                     /gene="fba"
                     /locus_tag="ACZPD4_04970"
     CDS             complement(6017..6868)
                     /gene="fba"
                     /locus_tag="ACZPD4_04970"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1378459.1"
                     /translation="MPLVTTNEMLKKAQAGHYAVGAFNVENMEMVMAVIKAAEEMNSP
                     VIMQTTPSTVKYAGLDYYLAMVRTAAERASVPVAIHLDHGSSFELAMQALRTGYTSIM
                     IDGSHGSFEENVALTRRVADACLPSQISVEAELGKVGGKEDDLEAENDSPYTDPQQAK
                     EFAERTNATSLAVAIGTAHGLYQGTPKLDFERLAAIREVVSIPLVLHGASGVPDDAVR
                     ESIRLGICKVNFATELRIAYSDGVKKYLAENPDGFDPKKYGAAGMENVTALVKEKISV
                     CGSEGQA"
BASE COUNT         2204 a         1248 c         1695 g         2013 t
ORIGIN      
        1 acaattttta ttatattgat tgccgctgtc ggatatatta tttttcaggg catcaaaaat
       61 tttcaactgc ataatatgaa tgtcggactt aaaaacaagg acagtgtcct tgtggaaaaa
      121 acggcagata tgtggatcac gagaaaactt cttggagagt atgtctgtga cctctataaa
      181 ttaagagtgt tatatgtgac gaaagacgaa gaaaaattcg agaaaatgct catacatatg
      241 ctggatacga catatccgcg tcctgatgat aagcaaagct ttttggaaac gtattttcat
      301 acattcctga tcaaaggtaa caggaaatat gcggactgga tcttaaagga aattaagaaa
      361 acaggggacg aggcatttat tcattacaac gagaatgcat atgcggtcat gcttgacggc
      421 agaacagatc tgatcgagga gatggacgag gatatcaatt caaagcggta ctatggattt
      481 gcactcggag tgatcttggt tatgatttca aagcaatatt ctgctctggg ggatgacaaa
      541 aatgcactga tttacatgca gagtgcaaaa gcttgtttac atcccaaagc tgtttatatg
      601 cccgttgtcg atcgttatct tagagaggct gaggctcagg agcaagatag ttaaagcaaa
      661 atactgcaga aaatgcagac agtaatatag aacaggagag atattatgat aggattaatc
      721 gttacaggac atgcaaattt tggttcgggt atgacaagct ctgtgaattt gatcgcagga
      781 gagcaggaag cataccgtta tgtagatttt cttccgacat atggaacaga agaactgaca
      841 gaagagattg cgaaagcaat ggatgagttg aaagattgtg aaggaattat tatctttaca
      901 gatctgatgg gtggatcgcc gtttaacgtt gctgcttcac tcggacacgg aaaagaaaat
      961 gtacgcattg ttgcgggtac aaacctgccg atgctcgtag agatcgtaat gtcgcgtaaa
     1021 ttcatggacg atctggacgg acttgtggag tctgtacttg agacaggaaa agagcaggta
     1081 acaaaatatg aattcaagca ggtagtacag gaagagtctg aagacggaat ttaaatcgac
     1141 tttttataat ggctgatatt cgcggaaaag acgatacagc cgtaagtctg aatattaact
     1201 gcatactata aaacacgctg caaaaagcag cacgaaagga gataaatcat gagtacaatt
     1261 tttggtatta cagaagacaa aatgaaagaa acatcttcaa catttacatt aacggagatc
     1321 tatcagcagc cggcgacatg ggagaagaca tgccgtcaga tcgaggagaa caaagaagaa
     1381 cttcagaaat ttatcgatca ggttatcaaa tgtgatgatt tcgatgtgat ccttacagga
     1441 gcaggaacaa gtgaatttgt aggaaatgca ttatttgcac atctttccgg tctgctgaat
     1501 cacaaagcaa aatcttacgg tacaacagat attgtcgcta cgccggaagc atatttgtct
     1561 cgcacgaaac cgactcttct gatcagtttc ggacgttcgg gaaattcacc ggagtctgtc
     1621 ggagcggtag atgcggcaga aagcgtatgt gataatgttt atcacctttt tgtaacatgt
     1681 aataaaaacg gagctctttc caaacgtgcg gcagagacac ataactgcta cgcaatcaat
     1741 ctgacagatg agacacatga tcagagcttt gcaatgacat caagctattc taacatgtat
     1801 cttgcaacat atctttgctt ccacctgaac gagctggaag agacagttga aaaagttcgc
     1861 aagatcgcag cagcaggaca gaacttcctt gacaatcatt acagtgtcgc tcagcagatc
     1921 gttgatgaat acaattttga acgtatcgta tatcttggaa gcaacacatt aaaaggaaca
     1981 tctcaggaat ctgcccttaa aatgcttgaa ctgacagccg gacgtgttgt tacaatgtat
     2041 gacacaccga tgggattccg tcacggaccg aaatcaatcg ttgacgatac aacattaaca
     2101 gttgtatatt tgagcgatga agcttataca agacagtatg aagtggacct tctcaaagaa
     2161 atgagcggac agagaaaagg aaacaagatc gtagttgtta tgagcagtgc agatgaagct
     2221 gtttcagcac ttgcagacta cacagttgta tatgacatga aagaagagaa tgaaaacatt
     2281 cttcttggat tggattatat cttgtttgca cagacactgg cagttcttaa atcactttca
     2341 cttgatatca caccggataa tccgtgtccg acaggtgaag taaaccgagt tgtaaaaggt
     2401 gttacacttt atccgtatac aagaaaataa gactgaccga aagaattacc ggaaatgcca
     2461 aaaggtatat ttcgggattg taagcggtcg ccaaggtttt ggaaaagtcc ggactttggt
     2521 ttgtcaccgt tacaaaaaga acatggtttg ccgaaaggca gaccatgttc cgataaggag
     2581 gataaaatat aattatggct attaacagtg aagttatcat atggacttgt gttacagttg
     2641 cggtacttgt cggcatctgt gcaatcgttc tgatcgcaat gtcttcaaga aacatgaaga
     2701 aaaaccgcga agcgatgcgc gatctgcaag gtgcaatcaa agttggagca cgcatcatgt
     2761 tcgcgggtgg tatttacgga aagatcgtta agatcaaaaa cgatgtgatc gatgtggaga
     2821 tcaacaaaag cactgtgatc caaatttccc gatacagcat tcaaaacgta atcacagact
     2881 aaaatatcat tcagatgtga tcagggtttg ttttggcggt tttggttttc ggctgctttt
     2941 ctggcatagt cgcttggcgt tgtgttaaaa tattttttaa acaacctcga aaagtagtgg
     3001 atagagctga aaccgagtct gtatgcgatc tcgctgattg tgtattgatt ctcgcggatc
     3061 agctctttac ttttttgaag tttaatattg agcagatagt tctttggcga ggtgttcagg
     3121 tgagtcttga acagcgcttg cagagaagaa cgtgagataa aaaattcatg acagatgtct
     3181 tctatcgtga gtggttcggt aagacgcttg ttcatgtaag caagaatctc agacagaagg
     3241 tcgttttgag ccgatttgct gtcagttaag agtgttttat ggttcattgt ctcatagaac
     3301 tgcatgatca gcaggagaac ttcctgaaga tagcagacca ttaaagtatg agtatagtag
     3361 gaatgttctt cgctttcgat caaaagtttc caaaggatct gctgcatttc gcttgtacag
     3421 tgaaatgtat gattaaggat atgcagagaa cgcctgtgat ttacttcaaa tacgactgtg
     3481 agatatgagc agccgttttg tacggataag tcggctttgt cagagcggta tatgatcaag
     3541 tcatgaggct gaagtgtgtt cgttccggaa gagagggtga tatccagact gccttcgtac
     3601 acatagatca gttcatatga tttgtgtgca ggctgtacaa tgtggcatgc tttggactgc
     3661 tttgtaaacc agcaatcaag gattcgcctt aagtggaacg gtacagatac aggctgatat
     3721 gtatacggag ttgacagtgg ttttgtaaaa tctgtcagtt gctgttcttt acagcagttt
     3781 ttcccgataa ggatattcca cgtaagagaa gaggccagtg gaatcacatt aaatgcggta
     3841 ttcggagcaa gttttaaatt gtgacgcaga ggaaatgttt ttaactccgt catgtcagga
     3901 tctgtagcga taagcaaaag tcccaatccg gcggggcatt ctatatatac ttcgcacgga
     3961 aaaagaaaga gagtatctag tttttttgaa gatgcagaaa actcccattg atttaaatta
     4021 tgtttttgag tgtcgagcgg ttcatcataa aacgccccaa aaggttcaaa tttatttgtt
     4081 atctgttgat ttgtcatgat gggatacctc ggttgtaaaa aacgatttgt actttttaaa
     4141 taatacaaca ttttttaacg aaaatcaaga tgtgggattt gtatgtcccg cgaaagacag
     4201 gaggaattgt aatgattatt caaagtaaaa aagtttggtt tgcagatcag tttgtagaag
     4261 cacagattga agtgaaagac ggaaaaattg tcgatatcta tgagtacgga actcatccgg
     4321 tagattgtga ttacggagat aaccgtattt tgccgggatt cattgacgtg cactgtcacg
     4381 gagcttacgg ctttgatacg aatgatgcga aagaagacgg acttcgttac tgggtgcgca
     4441 atattgtaga tgaaggagtg acggctcttc ttgcgacaac tatcacgcag agtgaagaag
     4501 tgttgacaaa tgccttgaaa aatgtggcga aagttgttga agacggttat gaaggagcag
     4561 agattttagg agttcacttt gaaggtccgt atcttgatat gaagtacaag ggagcacagc
     4621 cggaacagta cattgtaaaa cctacggtag agcagtttaa gaaatatcag gaagcggcaa
     4681 atggattaat aaaatatatt acgatggcaa cagagacaga tgatgatttc gcattgacac
     4741 gttactgtgc ggaaaacggg gtagttgtga gtatcggaca ttcggcggca acgagcaaag
     4801 aagcggtaca ggcatttgcc catggcgcaa gatcgatgac acatgtatat aacggaatga
     4861 caccgtttaa tcaccgtgcg aacggacttg taggcgctgc atatagaatg aagagcatgt
     4921 atggcgagat tatttgcgac ggcaatcatt caacaccgtt agcgctccat cagttctttg
     4981 aagcaaaagg acctcattac ggaatcatgg tcagtgatgc gcttatggca aaaggatcac
     5041 cggcaggatc gaaatttatt ttcggaggaa acgagattga gatttatgaa gacggaagcg
     5101 cacatctgac gtcttcaaaa ggtttggcgg gatcgactct tcatatcaat gaaggactgc
     5161 gtattctgat cgaagaagcg cttgttccgg tagatacggc aattaactca tgtacaaaga
     5221 acccggctgc ctgcctgaag atcgatgatc gcaaaggttc tattaaagtg ggattggatg
     5281 cggaccttgt tgttcttgac cgtgattatc aggtacttca gacatattgc atgggaaaag
     5341 caatgttaga taaaaactag aaaatgaaag attgtggaat gattaaaaat ggctaagaaa
     5401 aaatcgaaac cggtggagaa gatccgcctt tcaggaagaa atatttatac agataaaaaa
     5461 ggacgggtta ttttttacga tatgattacc aaaaaagggt atctcgtaga taaaaaacat
     5521 gaaaactcgg ctctgttttt taaaaaccgt tttgttgtac ttttgtttgc ggcaattttg
     5581 tttggggcaa cgtttttgac atggcttcag gctgcggttg cctgggcagt gatgatggcg
     5641 attgcggaat ttgcttttcg gcggggattt ttgaaaaagc tggaagtggt aaacgatgtg
     5701 gattttgaac gtcgtgtttc agcgcttcaa tacatcgtgg aaaacaaagc aaaaggaaaa
     5761 gtgatcgttc ttattgtttt gtatctcctt ttagcagtgc ttgtaatatt aaatgcatat
     5821 gtagaaaagt attctgttgg gttatgcgta ctcagcggag gaatcgcact tgtcggtgtg
     5881 tactttgcga ttttgcatat cgttgctctg actaaaatga aataaatgta gagaagatga
     5941 acataaaaat acagaccgga gaagtttgaa atctccggtc tgtattttta tgtttttgca
     6001 aaggagaagc ttatgattaa gcctgtcctt cagatccgca tacgctgatt ttttctttta
     6061 cgagagctgt aacattttcc atacctgcag ctccgtattt tttcggatcg aaaccgtccg
     6121 gattttccgc aaggtatttt ttcacaccgt cgctgtatgc gatacgaagt tctgtagcaa
     6181 agtttacttt acagatacca aggcggatgg attcgcgtac tgcgtcgtcc ggtacgccgg
     6241 aagctccgtg aagtacaagc ggaatagaca caacttcgcg gattgccgca aggcgttcaa
     6301 aatcaagttt tggtgtgccc tgatacagac cgtgtgctgt tccgattgca actgcaagtg
     6361 aagttgcgtt tgtacgttct gcaaactctt ttgcctgctg aggatctgta tacggagaat
     6421 cattctctgc ttcaaggtca tcttctttac cgccgacttt accgagttca gcttctacgc
     6481 tgatctgaga aggaagacat gcgtctgcca ctctgcgtgt cagggcaaca ttctcttcaa
     6541 aagagccgtg agaaccatcg atcatgatag aagtatatcc tgtgcgaaga gcctgcattg
     6601 ccaactcaaa gctgctgcca tggtcaagat ggatcgctac cggaacgctt gcgcgctcag
     6661 ccgctgttct taccattgca agataataat caagtcctgc atattttact gtggatggag
     6721 tcgtctgcat gatgaccggg gagttcatct cttcggctgc tttaataacc gccatgacca
     6781 tttccatatt ttccacgttg aatgcgccta ctgcgtaatg tcctgcctgc gcttttttca
     6841 gcatttcatt tgttgtaact aatggcataa tatcaacctc gattctttag aatttaatgt
     6901 aatgattaca ttattattaa ttattttcgg tataagtata gcacagaaca tgtgatttcg
     6961 cattttcatt ttgcactttc ttaaaaaatg caaatgattg tttaattaat ccaaagtagg
     7021 cgcaaattga aagtgctacg ataaatataa caaagcaaat tgaaaaggag agtttatcat
     7081 gcgtcaaaat ccattaaaaa aaattgttga attgcagaaa cagggtaaaa atgtcggaat
     7141 ctattctgtg tgcagcgcta
//
LOCUS       DBMDXF010000092         7145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2711_length_7145_cov_2.012777, whole genome shotgun sequence.
ACCESSION   DBMDXF010000092 DBMDXF010000000
VERSION     DBMDXF010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2711_length_7145_cov_2.012777"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            54..698
                     /locus_tag="ACZPD4_04975"
     CDS             54..698
                     /locus_tag="ACZPD4_04975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1378460.1"
                     /translation="MQTKKRIILGSASPRRRELLEQIGISFEVRVSDKEEVYHSLIPE
                     EIVKELALSKAENVADDLREKQEQVKQISFDKKNNVLLDTIVIGADTIVVSDGSILGK
                     PKDEADAVRMIRSLQGRSHKVYTGVAILDYDDEGKRKSVVHAVETEVFVNPMSDEEIR
                     EYAATGEPLDKAGAYGIQGQFAAYIERIDGDYYNVVGLPVSYVYRQLKEIAEKD"
     regulatory      980..1085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1125..1790
                     /locus_tag="ACZPD4_04980"
     CDS             1125..1790
                     /locus_tag="ACZPD4_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIR1378461.1"
                     /translation="MFDLLVNAEGGLTTAGYVVTISVGIVLFLAAVFFAGKRSKRQKM
                     TTKQLVFCAAAMALAFVTSYVKIFQMPWGGSVTLCSMLFIVLVANWYGVGTGVLVGLA
                     YGILQFIQEPYVLSFFQVCCDYILAFAALGVAGFFAKKKHGLLKGYIAAVLARGAFHA
                     LGGYLYWMDYMPDNFPKSLTAIYPIAYNYSFLLAEAAITLVIICIPAVAKGLGKVKQI
                     AAE"
     gene            2085..2804
                     /locus_tag="ACZPD4_04985"
     CDS             2085..2804
                     /locus_tag="ACZPD4_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378462.1"
                     /translation="MAEEMNMGEVVMVVDDNKEIVYSISELLKYEGYRVIPAYDGMEA
                     LNLLEQNPVDLILLDVMMPRLNGLSALMKLREKYRIPVIILSAKTEESDKVSGLVLGA
                     DDYVEKPYNPAELMARVKAHLRRYRAWGGELPKENDDQIINGGLVLDKKQRIVLVEGE
                     EVRLTATEYKILELLMEHPGQVFSSEQIYENVWQESANYTVENTVMVHIRHIREKIEI
                     DAKKPRYVKVVWGIGYKMEKY"
     gene            2779..4077
                     /locus_tag="ACZPD4_04990"
     CDS             2779..4077
                     /locus_tag="ACZPD4_04990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378463.1"
                     /translation="MDTKWKNIKSKAKSFLEKCWLSSTGIAVKLFCFVLAGFGIAFWY
                     SVVVYRTYHPTGESLFFGILGNALFGAGQMILTAFLIMRKFREWDPGEKDAYVKWQNR
                     WKIRNRRLQWIGGTALLIALWYMSYLMNGGMYIGTGWQYNYASGYVMILSALVQFVLA
                     GAVMNHFKKKQLDMIMCNFEKINQNRIKEALEIERKSLEKVSRSDQLRVDLITNVSHD
                     LKTPLTSMVGYIELIKREELSDIVRDYVDVISERAEKLKEMINSLFNLAKASSGNVEL
                     HPEPFEVNRMIEQIFADMDDRIKESHLEFVTDLTKEDTQLISDNSYVYRICQNLIENA
                     LKYSAKGTRVFVKTKLLQSEGKEEVWLEVTNTSGYPMDFTKEDIIERFARGDKARTGD
                     GNGLGLAIVSTYAKALGGEFDILIDCDQFKACLKFPKKTT"
     gene            complement(4233..5609)
                     /locus_tag="ACZPD4_04995"
     CDS             complement(4233..5609)
                     /locus_tag="ACZPD4_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378464.1"
                     /translation="MSTNIQKNQITEGIIWRQLLLFFFPILLGTFFQQLYNTIDSVIV
                     GQFVGKEALAAVGGSSAQIVAFVVGFFTGLSSGASVTIAQFFGAKDSRSANKGLHTAY
                     AIAAFGGLILTVSGIILTPFLLEMMNTPADIVADSAVYLRIYFAGIIFVLIYNMGSAI
                     LRATGDSKRPLYYLIVCCIINIVLDVLFVIVFHMGVMGVAVATLIAQAVSAVLVTLKL
                     MRSEGILKLSVKQIRFHGSILKMQLKLGLPTGFEAILFSITNIAIQSAVNTFGTDTTA
                     AWSAYGKLDAIFWMVSTAFGIAITTFVGQNYGAGKMDRVRKSTRVCLLMDFIVSAVLV
                     VFLIAARFLLFRLFTNDSHVIEIGCDMLVLITPWYIVYVFIEVIAGALRGVGNVVVPV
                     AITLLGVCVLRIAWLAAMLKISPTISAIILSYPVTWVITALAFIIYYIYITHKTIAGS
                     SKKHVPKL"
     gene            complement(5769..6317)
                     /locus_tag="ACZPD4_05000"
     CDS             complement(5769..6317)
                     /locus_tag="ACZPD4_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HIR1378465.1"
                     /translation="MKDYVIDVLMNIGVPAGIKGFTYICDAIELFNTDPYYPDGKISA
                     LYIDIAKKYHTTPSRVERSIRHAFDIALTKGDPDMVSRYLDLTNRQNSTLLRTLYLRI
                     GQERRRHQAERHHQQCNPQTCTSQTCEFKAQIYMEAMKTLSEEIESLFNRTLASVRDD
                     IHPTDDGQSERSCSGFPKSLKS"
     gene            6528..>7145
                     /locus_tag="ACZPD4_05005"
     CDS             6528..>7145
                     /locus_tag="ACZPD4_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378466.1"
                     /translation="MTMIRNKIRKRRQELNMSRVQLAEAVQVTPSAIANYENGISYPK
                     PDIFVALMITLEVDANYFYEEYVRNREIAIACKRAITQDEIYALEKYRQLTSSGKKLV
                     RMVIEEEYSRMQQEEWISFPCMKDMGGGIDAICKKRAEYFEFRVRKENMIDGMEFCLQ
                     LQTDRYEPVFRNGDVIALTEKKAGQNEIGIFLVNEVYYLRLLYQEN"
BASE COUNT         2194 a         1192 c         1811 g         1948 t
ORIGIN      
        1 ttgttgtata cttggagtaa ataaaataaa tagaagaaag cagagaagtg aaaatgcaga
       61 caaagaaaag aattattctt gggtcggctt ccccgagaag gcgggagctc cttgagcaga
      121 tcggaatttc atttgaagta agagtcagtg acaaagaaga agtttatcat agtctgatcc
      181 ccgaagagat tgtaaaagag cttgcgcttt caaaggcgga aaatgttgcg gatgatttac
      241 gggaaaaaca agagcaggtg aagcagataa gttttgataa gaaaaataat gtactgttgg
      301 atacgattgt gatcggagcg gatacgatcg ttgtgtcaga cggaagtatt ttagggaaac
      361 cgaaagatga ggcagatgcc gtccgtatga tccgaagtct gcagggaaga agtcacaaag
      421 tgtatacagg cgtggcaatt ttggattatg acgatgaggg aaaaagaaag tcggttgttc
      481 atgcagttga aacggaagtt tttgtcaatc ctatgagtga cgaagagatt cgggaatatg
      541 ccgcaacagg agaacctctt gacaaagccg gagcgtatgg tatacagggg cagtttgcag
      601 cttatatcga acggatcgac ggagattatt ataacgtggt cggactcccg gtatcttatg
      661 tgtatcgaca gttaaaagaa atagcggaga aggactgatg ggggcacagc gatgcccata
      721 taaaaggccg gacaggatga ttgttcaaat ttcttgacat tttttgtcaa agaggttatt
      781 atggatacgg tcaaagatgt aacagtttat cccacgccat tcgtaaaacc gcactgcgtg
      841 cttattgtgg ctgacgccac tgttttactc ataaataggc gcttagcaca tacagatgtc
      901 cgcttgcaaa aacatgcagg catgttttgt gcttcacaca cactgtatgg ctaaactgtt
      961 actaaaaaat tacatataga ttgatgacaa ggggagcttg tgttacaggc tgagaggaag
     1021 ttgggaactt cgacccataa cctgatttgg ataatgccaa cgtagggatt atgttatgag
     1081 tgtttatacc ttttgttttc aatctgagaa caggaggttt ttttatgttt gatttacttg
     1141 taaatgcaga aggcgggctg acaactgcgg gatatgttgt cacgatatcc gtcgggatcg
     1201 tactttttct ggcggcagta tttttcgcgg gaaagagatc taagcgtcaa aaaatgacga
     1261 caaagcagct agtattttgt gcggctgcaa tggccctggc gtttgttacg tcttatgtaa
     1321 agatttttca gatgccatgg ggaggcagtg tgacattatg cagcatgctg tttattgtac
     1381 ttgtggcaaa ctggtacggt gtcggaacag gagttttggt aggtcttgct tatggaatcc
     1441 tgcagtttat acaggaaccg tatgtacttt cttttttcca agtgtgctgt gattatattc
     1501 tggcatttgc agctcttggt gtggcaggat tttttgcaaa aaagaagcac ggactgctta
     1561 aaggttatat cgcggcagtt ttggcaagag gtgcgtttca cgcacttggg ggatatttgt
     1621 attggatgga ttacatgccc gataacttcc cgaaatcatt gacagcgatc tatccgattg
     1681 catataatta tagctttctt ttggcggaag cggcgattac gctcgtgatc atttgcattc
     1741 ctgccgtggc aaagggactc ggtaaggtaa agcagattgc ggcggaataa aataaaattc
     1801 acatgtgtaa agatgacagg cggttcggaa ataaagaaat ttccggatcg cctgttatcg
     1861 tttgtggtat aatgattgca cgttgtgcaa tccaagccga ttgcccggct tgccgccaca
     1921 aaaagtggcg gattataccg gcgatccact gcttggaaag gaaatgtatg aaaggaaatt
     1981 cccgacggcc ttttataaga aaaatttaag aattacatcg agtttttttt aacagtggaa
     2041 gtgtactgtt tatatcagat aaaggaaaac gagaggagag aatgatggca gaggaaatga
     2101 atatgggaga ggttgtcatg gttgttgatg acaacaaaga aattgtatat tcgatcagtg
     2161 aacttttaaa atacgaagga taccgggtga ttccggcgta tgatggaatg gaagcgttaa
     2221 acttgcttga gcagaatccg gtagatttaa ttctgcttga tgttatgatg ccacggttaa
     2281 atggcttgtc tgctcttatg aagcttcggg agaaatacag gattccggtt attattcttt
     2341 cggcaaagac ggaggaaagt gataaggtat cgggacttgt gcttggtgcg gacgattatg
     2401 tggaaaagcc ctataatccg gcggagttga tggctcgcgt gaaagcgcat ttgaggagat
     2461 acagggcgtg gggaggcgag cttccgaagg aaaatgatga tcagatcata aacggaggcc
     2521 ttgtacttga caaaaaacag cggatcgtac ttgtggaagg ggaggaagtg aggctgacgg
     2581 ctacggagta taaaatttta gaacttttga tggaacatcc ggggcaggtg ttttcttcgg
     2641 agcagattta tgaaaatgtc tggcaggaat cggcaaacta tacagtggaa aacacggtaa
     2701 tggttcatat cagacacatt cgggagaaaa ttgagattga tgcaaagaaa ccaagatatg
     2761 taaaggtggt gtggggtatt ggatacaaaa tggaaaaata ttaagtcaaa ggcgaaaagt
     2821 tttttagaga aatgctggct tagcagtaca gggattgcag tgaaactatt ttgttttgtg
     2881 ctggcagggt ttggaatcgc attctggtat agtgttgtag tgtaccgtac gtaccatccg
     2941 accggagaat cattgttttt cgggatattg ggaaatgctt tatttggagc agggcagatg
     3001 atactgaccg catttctcat tatgaggaaa ttccgtgaat gggatccggg agaaaaggat
     3061 gcgtatgtga aatggcagaa caggtggaaa atcaggaatc gcagactgca gtggataggc
     3121 ggaacagctc ttttgatcgc tttgtggtat atgtcgtact taatgaacgg cggaatgtat
     3181 ataggaacag gatggcaata caattatgca tcgggttatg tcatgatttt atcggcgctc
     3241 gttcagtttg tgcttgccgg agcggtgatg aatcacttta aaaagaaaca gttagacatg
     3301 atcatgtgta attttgaaaa gataaatcaa aacaggatca aagaagcgct agagatcgag
     3361 cgaaagagtc ttgaaaaagt atcgcgcagc gatcagctca gagtagatct tattacaaat
     3421 gtttcccatg atctgaaaac gccgcttacg tcgatggtcg gctatattga actgatcaag
     3481 agggaagaat taagcgatat tgtccgcgat tatgtggatg tgatttcaga gcgggcagag
     3541 aaactaaaag aaatgatcaa cagtttattt aatcttgcaa aagcaagcag cggaaatgta
     3601 gagctgcatc cggagccgtt cgaggtaaat cggatgatcg aacagatctt tgcggatatg
     3661 gatgacagga taaaagaaag tcatttggaa tttgttaccg atctgacaaa agaagataca
     3721 cagcttatat cggacaattc ttatgtttac agaatctgcc agaatctgat tgaaaatgcg
     3781 ttgaaatatt ctgcaaaggg aacgcgtgtc tttgtgaaaa cgaagctttt gcagtcggaa
     3841 ggaaaagagg aagtatggct ggaagtaaca aacacatcgg gatatccgat ggattttaca
     3901 aaagaagata ttatcgaaag gtttgcaaga ggagataagg caagaaccgg agacggaaac
     3961 ggacttggac tggcgatcgt aagtacttat gccaaagcgc tcgggggaga atttgatata
     4021 ttgatcgact gcgatcagtt taaagcctgt ttgaaatttc cgaaaaagac aacataattt
     4081 tgattattat gcagaagaat gcagaaaagc aaaaaatcac ccgtttctta tgtgagtata
     4141 ttactttatg atgattcggg taattttttg cttttttact ctcgcagcgc agatagtcca
     4201 agtccgggtc tatctaagat tttatcggct gcttacaatt ttggaacatg ctttttagaa
     4261 cttccggcga tagttttatg ggttatgtat atatagtaga tgataaatgc aagcgctgtg
     4321 ataacccatg ttaccggata gcttaaaata atcgccgata tcgtcggtga gattttcagc
     4381 atggcggcaa gccatgcgat gcgaaggacg cagacgccaa gcagagtaat ggctaccgga
     4441 acgacgacgt ttccgactcc gcgcagcgct ccggcgataa cttcgataaa gacatataca
     4501 atgtaccatg gcgtgatcag aacgagcatg tcgcatccga tttcaattac atgactgtcg
     4561 tttgtaaata accggaataa aaggaatcgt gcggcgatca gaaagacaac aagtacggcg
     4621 gaaacaataa aatccataag aaggcatacg cgggtacttt tgcgtacgcg atccattttt
     4681 ccggcgccgt agttttgccc tacgaatgtt gtgatcgcaa ttccgaacgc agtgctgacc
     4741 atccaaaaaa ttgcgtccag ttttccgtag gctgaccatg cggcggttgt gtccgtgccg
     4801 aaagtgttga cagcagattg aatggcaata tttgtaatag agaacaggat cgcttcaaat
     4861 ccggtcggca gtccgagttt taactgcatt ttcaaaatcg aaccgtggaa acggatctgt
     4921 ttgacggaaa gttttaatat gccttcagag cgcatcagtt ttaaagtgac aagtacggcg
     4981 cttacagcct gtgcgatgag ggtggcaacg gcaactccca taacacccat atggaatacg
     5041 ataacgaata aaacgtcaag cacgatgttg atgatgcagc agacgatcaa ataatagagc
     5101 ggacgtttgg aatctccggt ggcgcgcagt attgccgatc ccatgttgta aatgaggacg
     5161 aaaatgattc cggcaaagta gatccttaag taaacggcgg aatctgctac aatatcggca
     5221 ggcgtattca tcatctctaa aagaaacgga gtcagaatta ttcctgatac ggtaaggatc
     5281 aaacctccga aagcggcgat cgcgtaagct gtgtgaagac ctttatttgc agagcgggag
     5341 tctttcgcac cgaaaaattg tgcaatcgtt acggatgccc cggaagaaag cccggtaaaa
     5401 aatccgacta caaaagcaac gatctgagca gaagaacctc ctactgcggc gagagcttct
     5461 tttccgacga attgaccgac gataacactg tcaatcgtgt tgtatagctg ttgaaagaaa
     5521 gtcccgagta aaatgggaaa gaagaaaagt agaagctgcc gccaaataat gccttctgta
     5581 atctggtttt tctgaatgtt ggtagacata gaaccatctc ctttcaataa ttttcggtat
     5641 taggcgtttg tgaaacgcct aatattatat atgaaaagcg aggtaaaacc aagtgaaatt
     5701 caaacatatg ttgttttgcg gagctgtttt caaagatgat aaaaataaaa taagaaagag
     5761 ccgagtcttt aagattttaa agattttgga aatccggaac agctccgttc actttgtccg
     5821 tcgtctgtcg gatgaatatc atctcttaca gaagcaagag tgcgattaaa caaagattcg
     5881 atctcttcgg ataaggtttt cattgcttcc atatatatct gtgctttaaa ctcacaagtc
     5941 tgtgaggtac aagtttgtgg gttgcattgc tgatgatgcc gttctgcctg atgtctgcgg
     6001 cgttcttgcc cgattcgaag atagagtgtc cgcaatagcg tggagttctg tctgtttgtc
     6061 agatccagat agcgggatac catatcagga tcaccttttg taagtgcaat atcgaaagcg
     6121 tgtcgtatgg aacgctctac tcttgatggt gttgtgtgat actttttagc gatatcgata
     6181 tagagtgctg atatctttcc gtcaggatag tatggatcgg tattaaataa ttctattgcg
     6241 tcgcaaatat aagtaaaacc tttgattcct gccggaactc cgatgttcat cagtacatct
     6301 attacataat ctttcatcat gcccctcgat tctagttgtt aatattacct aagaaaattg
     6361 attcatatta agaaaatata ccaattttac ggctatttct acatcagttt acgacagatt
     6421 taataaaatg tcaatgagaa actacaataa tcttgaaaaa gcgattgctt ttacaggaaa
     6481 tttgtaatat actttgaata atatgtggtg aagagagggt taaaatcatg acgatgatca
     6541 gaaataagat tcgtaagcga cgtcaggagt taaacatgtc aagagtacag cttgccgaag
     6601 cggtgcaggt tacaccgtct gctattgcaa attatgagaa tggaatcagc tatccgaaac
     6661 cggatatttt tgttgcgctg atgatcacgc ttgaagtaga tgcgaattac ttttatgaag
     6721 agtatgtaag aaacagagag attgccatcg catgtaaaag agcgatcaca caggatgaga
     6781 tatatgctct tgaaaagtac aggcagttga cgagcagcgg gaaaaaactt gttcgtatgg
     6841 tgatcgaaga agagtattcg agaatgcagc aggaagaatg gatttctttt ccgtgtatga
     6901 aagatatggg aggagggatt gatgcgattt gtaaaaaaag agcagaatat tttgaattcc
     6961 gtgtacgcaa ggagaatatg atagacggca tggaattttg cctgcagctt cagacagaca
     7021 gatacgagcc tgtttttcgg aacggggatg tgatcgcatt gaccgaaaaa aaagcaggtc
     7081 aaaatgaaat aggaattttc ttagtaaatg aagtttatta tttgagatta ttatatcagg
     7141 aaaat
//
LOCUS       DBMDXF010000093         7075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2742_length_7075_cov_2.394895, whole genome shotgun sequence.
ACCESSION   DBMDXF010000093 DBMDXF010000000
VERSION     DBMDXF010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_7075_cov_2.394895"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="ACZPD4_05010"
     CDS             <1..237
                     /locus_tag="ACZPD4_05010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1378467.1"
                     /translation="WANQANGMLQLAVWSMLRELNVGASLQHYNPVIDARVKENFDIP
                     ENFKLVAQMPFGGIVSEPDKKEKEDIDKRVTVLR"
     gene            572..2578
                     /locus_tag="ACZPD4_05015"
     CDS             572..2578
                     /locus_tag="ACZPD4_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1378468.1"
                     /translation="MIITGTSVAGAQEHPQFTADQTEIRLAKEELQTVQLSIEEYLKE
                     CWKNKQEVIDVSAYKLNEEELNKVIFGIHYENPEYYWVLRNTAINKDEETGFVETYYQ
                     YYEGTTGKPLDRSEELEREWEVVKEKTKYCKTDIEKALVVHEHLCDTIVYSSRLQAAA
                     HDIEGAIFEKEAVCEGYALAYKYYMNRLEIPCKIVSGTSNGQSHAWNQIQLNGNWYMV
                     DPTWDDVANSHDVKHQYFLCSENKFPNHVWNKESELYETCSDTTYDNLDWKNDLQGMY
                     AYQGGLYRSKSLIVGGKEVSGIWRIDAENIEKEAELVLPITDQWQLNSVNVVRGYSQL
                     SYYDGMLYYNTPRAVWRWNFDPESEPEKVFELDQSIPGEIWDAEAANGKIYYETGVYE
                     KGERQKGQYVFDEDYRKAKHPIAVTKPVMTVVMGGENVFLQSAAPGNVTYTSKNPEIC
                     DVQVVWKDESCQLIPKKAGETIVTVHADPTDHYAEGAVDVKVIVKERDDIEEKITLQY
                     EAGDGGSIAAVDAATGKILENGAKIKPNAEVQFTASPNSGYSVKNWVVNGEVYKENGI
                     VYTGMELKRAITASSDSVKVEFVKDEPAFVKGDVNLSGKVEIGDVREALRSICKKTEL
                     TALQKQAADVNENGNVDIEDLRKILRFVCGKIDQLNMEESGASK"
     gene            2575..5169
                     /locus_tag="ACZPD4_05020"
     CDS             2575..5169
                     /locus_tag="ACZPD4_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1378469.1"
                     /translation="MKILIIGGVAAGTKTAAKLKREDRNIDVTVITKDKDISYAGCGL
                     PYYVGGLIEGRDELIVNTPQKYSALTGVEVRTGKEAVALDAEKKQVTVQDVQTGEKEV
                     CSYDRLVVAVGASPAILPIEGKELAGVFKMRTPDDAEGIRTYAEQNNVKKAVVIGAGF
                     IGLEAAENLQAKGIQVTVIDFADQILPNIFDPEMALYAKRHLIRQGIRVLTGTKAEQI
                     YERGTQGRVAGIKTSAGNLPCEMIIMAAGIRPNTEFLNDSGIEMFKGTILTDDQMKTN
                     LDDVYAAGDCVMVKNRLTGKRQWSPMGSSANLEGRTLAQVLAGAQKSYPGVLGTGVVK
                     LPGLNAGRTGLTEAQAKEAGYDVVTALVPTDDKAHYYPDASFFITKLIADRSTRKLLG
                     VQVFGPGSVDKMVDIAVMGLNMGAVLDDFENADFAYAPPFSTAIHPFVQAVYVLMNKL
                     DGTIVSMTPAEYAAGKAEGYTVVDVAPEPSIRGAVYVNLGAVNGEIKGLGKEEKLLLV
                     CAKGKRGYFLQNRLRHYGYTNTVVLEGATFFNDVKVKNNIEEAVSKEDETRVKALGFL
                     KDKRTPDKFNGRVITRNGKITAEEAHTIAEAAQLYGSGEVTMTSRLTMEIQGVPYDNI
                     EPLREYLMQAGLEMGGTGSKVRPVVSCKGTTCQYGLIDTFALSEEIHERFFHGYSDVK
                     LPHKFKIAVGGCPNNCVKPDLNDLGIIGQKVPWVDLEKCRGCRICQVEKNCPIHAAKM
                     VDGKIVIDENVCNHCGRCISKCPFGVTEEFVSGYRVYIGGRWGKKVARGRYLEKVFTD
                     KEEVLDIVEKAILLFREQGITGERFADTVERLGFENVQEQLLGDGLLARKDENIRAQK
                     HLKGGATC"
     gene            5371..>7075
                     /locus_tag="ACZPD4_05025"
     CDS             5371..>7075
                     /locus_tag="ACZPD4_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1378470.1"
                     /translation="MQSEVQLAEANSKEKLLPLDQYLKDCWAKKQTAIDLTAYKITHE
                     ELNKVIFGIHYEEPEYYWTIRNDITDTDPQTGLLKTYYPYYSGETGSPFDRTEELERE
                     WEMVEEMTENCTTDLEKALVVHDHLVRTIQYSASLGAFVAHDIEGAIFEKKCVCEGYA
                     LAYKYYMNRLNIPCKVVSGVSKGQPHAWNQIKINGKWYFVDATWDDGSCVLEEKSHPV
                     KHEYFLKSETEFSDHTWNREGYEICNDTTYDNVEWKWVSRKMAAYKGGLYVAGSFPRD
                     GVIKSGIWRYDSEDPTQKGELVVEIEDEWPVSQYNKGKGCMEIAYYDGMLYYNTPKAV
                     WKWNFDKNTEPEKVFELEENVSGSIWYLHVADGKVYYETSLYEKNEKEKREYVIDVNY
                     QKVKHPIAVTSPVMTVELGGNAKEVFLQGAAPGIVTFKANNPDICDVEEAYADRSCKL
                     IPKKAGEATVTVHATATDHYLEGSVDVKIIVKGDSSTEQKITLQYESGSNGSLRAVNA
                     ATGENLSNGAQILPNTEVQFMASPNEGYSVKNWTINGEVYKENGQVYTGTTMKYAITA
                     SS"
BASE COUNT         2293 a         1169 c         1997 g         1616 t
ORIGIN      
        1 tgggcaaatc aggcaaacgg gatgctgcag cttgctgttt ggtctatgct aagagagttg
       61 aatgtcggcg cttcgctgca gcattataat ccggtaatag atgcacgggt gaaggaaaat
      121 tttgatatac cggaaaactt taaactggta gctcagatgc cgtttggagg catagtcagc
      181 gaacccgata aaaaagaaaa agaagatata gataaaagag taaccgtgtt aagatagcgg
      241 ctcatcagaa tagaatcaaa taaaaacata aaatccccct tttcggatca tccggagagg
      301 gggatttttg caccgtcata aacgaggaaa acagttcgga gaaccgaagg agaaaaggaa
      361 taaaagaggg gaaaataatc gtcagaaaaa agggtgaaaa tatgcggaat atggttgaca
      421 attatattcc taaatgttag cctgtctgta taatatcggt ctgtaaaaca gaagcggaag
      481 agcgtagaga aaagggaaaa agaaaaatga atatggatta tggaaaaagg actggaaaaa
      541 gcgggttgga gcgctagcac tctgtgcagg aatgataata acgggaacgt ctgtcgccgg
      601 agcacaggag catccgcagt ttacggcgga tcaaacggag atacgattgg caaaggagga
      661 gctgcaaacg gtgcagcttt ctatcgagga atatttgaag gagtgctgga agaacaaaca
      721 agaggtgatt gatgtaagcg cgtataaact gaatgaagaa gaactgaata aggtcatctt
      781 cgggatacat tatgaaaatc cggagtatta ttgggtgttg cgcaacactg ctattaataa
      841 agacgaggaa acaggcttcg tagaaacgta ttatcaatat tacgagggaa cgacgggaaa
      901 acctcttgac aggagcgaag aactggaacg ggaatgggaa gttgtaaagg aaaaaacgaa
      961 atattgtaaa acggatatag aaaaagcgtt agtagtacat gagcacttgt gcgatacaat
     1021 cgtgtattct tccagactgc aggcggcggc tcatgacatt gaaggcgcaa tttttgagaa
     1081 agaagctgtg tgcgagggat atgcgcttgc gtataaatat tatatgaatc gtttggaaat
     1141 tccgtgcaag atcgtaagcg gaacgtcaaa tggtcagtct catgcgtgga atcagattca
     1201 gcttaacggg aattggtaca tggtagatcc gacatgggat gatgtggcga acagtcatga
     1261 cgtaaagcat cagtattttt tgtgcagtga gaataagttt ccgaatcatg tatggaacaa
     1321 ggaaagcgaa ctgtatgaaa cttgcagcga tacgacgtat gacaatttag actggaaaaa
     1381 tgatcttcag ggaatgtacg cttatcaggg cggactgtac cgctctaaat cgttgatcgt
     1441 cggcggaaaa gaagtgagcg gtatatggcg gatcgatgcg gaaaatatag agaaagaagc
     1501 ggaattagtg cttccgatca cagatcagtg gcagctgaac tctgtcaatg tagtaagagg
     1561 atattcacaa ctttcttatt atgacggaat gctttactac aatacgccgc gggctgtatg
     1621 gagatggaat ttcgatcctg aatcagaacc tgagaaagta tttgaactgg atcagagtat
     1681 tccgggcgaa atatgggatg cagaggctgc aaacgggaag atttattacg agacaggcgt
     1741 ttatgaaaaa ggagagagac agaaggggca atatgtgttt gacgaagatt accggaaagc
     1801 aaagcatccg atcgcagtaa ccaaaccggt tatgacagtt gtaatgggcg gtgaaaatgt
     1861 gttcctgcag tcggcggctc cggggaacgt aacatatacg tcgaagaatc ctgagatctg
     1921 cgacgttcaa gttgtgtgga aagacgagtc ctgtcagctg attcctaaaa aagccgggga
     1981 gacgatcgta acggttcatg cagatccgac ggatcattat gcggaaggtg cggtagatgt
     2041 aaaagtgatc gttaaagagc gggatgatat tgaagaaaag atcactcttc aatatgaagc
     2101 cggagacgga ggaagtatag cagcagttga tgcggcaaca gggaaaatac ttgaaaacgg
     2161 agcaaagatc aaaccgaatg cagaagttca gtttacggcg tctccaaatt ccggttattc
     2221 tgtgaaaaac tgggttgtaa acggagaagt atataaggaa aacggcatcg tttatacagg
     2281 catggaatta aagcgcgcga tcaccgcttc atctgacagc gtgaaagttg aatttgtaaa
     2341 agatgagccg gcatttgtga agggcgatgt aaatttaagt gggaaggtcg agatcggcga
     2401 tgttcgtgaa gccttgagaa gtatatgcaa aaagacagaa ctgacagcgc ttcagaagca
     2461 ggcggcggat gtaaatgaaa acggaaacgt tgatatagaa gatttaagga agatcttaag
     2521 atttgtctgc ggaaagatcg atcaactgaa tatggaagaa agcggggcgt caaaatgaag
     2581 atattaatca tcggcggagt ggctgccgga acaaaaactg ctgcgaaatt gaaacgggaa
     2641 gacagaaata ttgatgtgac tgtgattacg aaagataaag atatctctta tgcgggatgt
     2701 ggtctgccgt actatgtggg cggactgatc gagggcaggg acgagctgat cgtgaatact
     2761 ccgcagaaat attccgcgct gaccggggtt gaagtaagaa cggggaaaga agcggttgca
     2821 ctcgacgcag aaaaaaagca agtgacggtg caggatgtac agacaggcga gaaagaagtc
     2881 tgttcctacg atagattagt agttgcagta ggagcatctc ccgcgatcct tccgatagaa
     2941 ggaaaagaac tggcaggcgt atttaagatg agaacgcctg acgatgcaga aggaatccgt
     3001 acatatgcag aacaaaacaa tgtgaaaaaa gcggttgtga tcggagctgg tttcatcgga
     3061 cttgaggctg cggagaattt acaggcaaaa ggaatccagg tgaccgtcat tgattttgcg
     3121 gatcagatcc tgccgaatat ttttgatcct gagatggcgc tctatgcgaa aagacatctg
     3181 atccggcagg gaatccgcgt gcttacagga acgaaagcgg agcagatata tgaaagaggt
     3241 acacagggcc gtgtggcggg aattaagacg tctgccggaa atctgccgtg tgaaatgatc
     3301 atcatggctg cgggaatccg tccgaatacg gagtttttaa atgacagcgg tatagagatg
     3361 ttcaaaggaa cgattcttac agacgatcag atgaaaacga atctggatga tgtatatgcg
     3421 gcaggagact gtgtgatggt gaagaaccgt ctgacgggaa aacgtcagtg gtcaccgatg
     3481 ggatcttcgg cgaatctgga aggacgtaca ttggcacagg tacttgccgg agcgcagaag
     3541 tcgtatccgg gagttctcgg cacaggcgtt gtgaagcttc cgggtctgaa tgccggaaga
     3601 acaggactga cagaagcgca ggcaaaagaa gcgggctacg atgtagttac ggcgcttgtt
     3661 ccgacggatg ataaagcgca ttattatccg gacgcttcgt ttttcattac gaaactgatt
     3721 gcagaccgga gtacaagaaa actgcttggc gttcaggtgt ttggtccggg ctctgttgat
     3781 aagatggttg atatcgcagt gatgggattg aatatgggcg cggttctgga tgattttgaa
     3841 aatgcggatt tcgcatatgc accgccgttc tcaacagcaa tccatccgtt tgtgcaggct
     3901 gtttatgtat tgatgaacaa actggacggt acgatcgtca gcatgactcc tgcggagtat
     3961 gccgcaggaa aagcagaagg ctatacggtt gtcgatgttg cgccggagcc gtcgatcaga
     4021 ggagcggtat atgtaaactt aggagctgta aacggcgaaa tcaaaggact tggaaaagag
     4081 gagaaactgc ttcttgtctg tgcaaagggc aaaagaggat attttcttca aaaccggttg
     4141 cgtcattacg gctatacgaa tacagtcgtt cttgaaggag caacattttt caatgatgta
     4201 aaagtgaaaa ataatataga agaagctgtc tctaaagaag acgagacgag agtaaaagcg
     4261 cttggatttt taaaggataa aaggacgccg gataaattta acggacgtgt gatcacaaga
     4321 aacggaaaga ttacagccga agaagcgcac acgattgccg aggcggcgca gttatacgga
     4381 agcggagaag tgacaatgac ctctcgtctg acgatggaga ttcagggcgt tccttatgac
     4441 aatatagaac cgctgcggga atatctgatg caggcaggac ttgagatggg aggaaccggc
     4501 tcaaaagtcc gtccggttgt atcgtgcaag ggaacgactt gtcagtacgg gctgatcgat
     4561 acgtttgcat tgtccgaaga aattcatgaa agatttttcc atggatacag cgatgtgaag
     4621 ctgccgcata aatttaagat cgcggtagga ggatgtccga ataattgtgt aaaaccggat
     4681 ctgaatgatc ttggaattat cggtcagaaa gttccgtggg tcgatcttga aaagtgccgt
     4741 ggctgccgga tttgccaggt agaaaaaaat tgtccgatcc atgcggcaaa aatggtggac
     4801 ggaaagattg tgatcgacga aaatgtatgt aatcactgtg gaaggtgcat cagtaaatgt
     4861 ccgtttggtg tgactgaaga atttgtttcg gggtatcgcg tatatatagg cggacgctgg
     4921 ggcaagaaag tagcgcgggg aagatacctt gaaaaagtgt ttacagataa agaagaagtg
     4981 cttgatattg tggaaaaagc aattcttttg ttccgggaac aggggatcac gggagagcga
     5041 tttgcggata cggttgaaag actcggattt gaaaatgttc aggaacagct tttgggagat
     5101 ggactgttag cccgcaaaga tgaaaacata agagcgcaga aacatttgaa aggcggagca
     5161 acttgttaag aattttataa aacgatagag tgcagcatag cgactcataa gacagattga
     5221 aggtaaaggg gaaaaagaaa aatgaacaaa aactatgagg catggaaaag aggttggaaa
     5281 aaacgagcgg gcgcactggc gctttgcggc ggaatattga ttgcggggac gtccgtcgta
     5341 ggagcacagg aaaacccgta gcaggcggca gtgcagtcag aagtacagct tgcagaagca
     5401 aactctaaag agaagctgct tccgttagat caatatctga aagactgctg ggcaaagaag
     5461 cagacggcga ttgatttaac cgcgtacaaa ataacgcatg aagaattgaa caaggtgatt
     5521 ttcggtatcc actatgagga accggaatat tattggacga tccgaaacga tataaccgat
     5581 acagatccgc aaacaggact tttgaaaact tattatcctt attattccgg cgagacagga
     5641 agtccttttg accgtacgga agaattggaa cgggaatggg aaatggttga agagatgacg
     5701 gaaaactgta cgactgatct ggaaaaagcg cttgtagtgc atgatcatct ggttagaaca
     5761 attcagtaca gtgcgtcatt gggcgctttt gtggcccatg atattgaagg tgccattttt
     5821 gagaaaaaat gtgtgtgtga aggatatgca ttggcgtata agtattacat gaatcgcctg
     5881 aatattccgt gtaaagtagt gagtggagtt tcaaaaggcc agcctcatgc gtggaatcag
     5941 attaaaatta atggaaaatg gtattttgta gacgcgacat gggatgacgg aagctgtgtg
     6001 ttggaggaaa aaagccaccc tgtaaaacat gaatatttcc tgaaaagtga gacggaattt
     6061 tcagatcaca catggaacag agaaggctat gaaatttgta acgatacaac ttatgacaac
     6121 gtagagtgga aatgggtttc cagaaaaatg gccgcatata aaggcggttt atatgttgcc
     6181 ggatcattcc ccagagacgg agtgataaag agcggaatat ggaggtatga ttcggaagac
     6241 ccgactcaga agggagaact ggtcgttgag atcgaggatg agtggccggt gtcacagtat
     6301 aataaaggca agggttgtat ggaaattgca tattatgacg ggatgcttta ctacaacact
     6361 ccgaaagctg tatggaaatg gaactttgat aagaatacag agccggaaaa agtttttgag
     6421 ttagaagaaa atgtttcggg cagtatctgg tatttgcatg tagcggacgg caaagtttat
     6481 tacgagacgt ctctttacga aaagaatgag aaagaaaagc gggagtatgt aattgatgta
     6541 aactatcaga aagtgaaaca cccgatcgct gtgacaagtc cggtaatgac agttgaactg
     6601 ggaggaaatg caaaagaggt attcctgcag ggggcagctc cgggaatcgt gacttttaaa
     6661 gcgaacaacc ctgacatttg tgacgtggaa gaggcgtacg cagacagatc gtgtaagctg
     6721 atcccgaaaa aggcgggtga agcgactgta actgtgcatg cgacagctac agatcactat
     6781 ttggaaggct cggtagatgt aaaaattatt gtcaaaggtg attcaagcac agaacagaag
     6841 atcacgcttc agtatgaatc cggttcaaac ggttctttaa gagcggttaa tgcggcaaca
     6901 ggagaaaatt tatcaaatgg agcgcagatt cttccgaata cagaagttca atttatggca
     6961 tctccgaacg aaggttattc tgtgaaaaac tggacgatta acggagaagt gtacaaagaa
     7021 aacggtcagg tttatactgg tactactatg aaatatgcga ttacagcttc atcag
//
LOCUS       DBMDXF010000094         7048 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2753_length_7048_cov_2.303296, whole genome shotgun sequence.
ACCESSION   DBMDXF010000094 DBMDXF010000000
VERSION     DBMDXF010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_7048_cov_2.303296"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_binding    170..381
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            655..2097
                     /gene="proS"
                     /locus_tag="ACZPD4_05030"
     CDS             655..2097
                     /gene="proS"
                     /locus_tag="ACZPD4_05030"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1378471.1"
                     /translation="MAKEKKLVQSITSRDEDFAQWYTDVVREAKLCDYSGVKGCLNYL
                     PNGYAIWENIQSDLDKRFKDTGVENVYLPVLIPESLLQKEKDHIEGFAPEVAWVTHGG
                     AEPLQERFCIRPTSETLFCDVWSKTVQSYRDLPKVWNQWCSVLRWEKTTRPFLRSREF
                     LWQEGHTIHATYEEAEERTKLMWKVYKDFVEENLAIPVVAGRKTESEKFAGAQDTYTI
                     EALMHDGQALQSATSHFFGNSFPDAFDIKYADKNNELHSVYETSWGLSTRIIGAIIMV
                     HGDDSGLVLPPRIAPVQTRVIPIAQHKEGVLDKANELLDALNKAGYRAKIDDSEKSPG
                     WKFSEQEMLGIPTRIEIGPKDIENGQVVVVRRDTREKIVVAIDEITTKLGEILETIQA
                     DLYAKAKAFLDDHISSAVTMDEMKDAVSEKKGFVKAMWCGDEACEDEIKAQTGGVTSR
                     CIPMEEEHLSDVCVCCGKPAKHMVYWGRAY"
     gene            2391..6857
                     /locus_tag="ACZPD4_05035"
     CDS             2391..6857
                     /locus_tag="ACZPD4_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR1378472.1"
                     /translation="MKKRKYLALLLAAGMIAQTALSTGGVVQVQAAEDTYVKTDELTD
                     NDTAAPKKDSVIPSKNQYEYQKQELAAFCHFGMNTYTGSEWGNGKENPNQFQLTNDFD
                     EETYVKAIHDAGFKKLIITAKHHDGFCIWPSEMTTHDTETAGYEGDVLEELSRACTKY
                     NIDMGLYLSPWDVNSEYYGYKDKDGKKLVGADGQPLNGKTWDQVKQEDALDYNEYYDN
                     QLKEILGNDKYGNNGHFKEVWMDGAKDTNDKSNAQDYDFQRWFKTIQQYEGKASGKYE
                     DDCLLFGAESYTTVRWIGNERGYAAEETWAKSTIDRENNTINSNSRDGYTKGFPNGNQ
                     WTVPEADTKITSGWFWGESKKTPLSMEQLAGIYFGSVGHNATLLLNVPPNKQGTVDAD
                     ILARVAEFGKAVQNTFDKNLAEKASVSATEVRGNSKKYSPENLLDGNDETYWTVGDGT
                     TSGKVLIDLGESKKFDVVSIEEAIQFGQRIGSFKVEYKNGNGEWKTFDQGTTIGAKRL
                     CRKKAVKADKLRITVTAHNQAENKVPILSEIGVYEAAEGFELGTGIPSGLQTKDDRGF
                     TLSSGWHQETNDQMIEGTGIWINGNGNGANAPYAETKFKGTKAWVIGTIDQKHGPADV
                     YIDGKKVASINTYSATRKLGQILYETNTLEDKEHTLKIVNTGSNTQAVGLDAVAYLDN
                     GGKGMVELEKDAYRVNEDTKYPIKLKRVGGTNGELTVQFEVAPGSAYQKHFDADGNMT
                     VTFKDGQEEAEAFVTTKRVKEKEGDVYFSAYLSAPTEDVLLGFITKAKITIADTESYD
                     KQELLDKIKETEKAGYQESLYTTASYKNYVDALKAAKEAAKKANLTQEEAAKACAALD
                     AAIASLTKRSTFSETDRLQFPKVKGRSTTVEAEYFVLDSSKAEKGKEVRIQPDTGASN
                     GAKVGWFEKGNVIKAPFHAEKEGKYTVTVTYQSGRDSGNPNKINWGGTNVDSGSVNVV
                     GKNTQNPVFEEKSFDINVTKAGDGELVLTADASSSPNIDKFVITAKEVSAATYKITAT
                     AGEHGKIEGVTGTEVTVEEGASHTFKFVPDENYAVKDVIVDGNSIGAVDSYTFDDVTA
                     NGHTIEVQFEKAVYAEDNRFVFPTDGNTKTAEAERFEIHDVKDPTDGEWHCGVTAKDW
                     ASGGKIVNAMNKGDTITLYYDAPQTGEYTVTVYYRSGNPQNGLTWAEKDGKIKSGNVT
                     AGAGDSAAATHTTEFTLNVETAGPGCLVFTAPDAKAPQLDKFDIKSPGTPVPPISAVE
                     LEAAVEAAKGLDLNDYKDDDAKTAFTAKLAEAEKLLADIAGGSFAGTQDEVNQMAKEL
                     KAAQDALNEKEQTPQVNKDTLKEAIGKAEALDLTQYKEEGKAEFNAALQNAKAVLEKA
                     DADQDEIDQATLALNTAIQNLKPVDGGSGDGNGEGNGNGSTGGNGNGSGSTGGSGNGS
                     GNTGGNGSTGGSGTGNSAVQTGDSSNLFVYAGTLLLAAAAFVFGFRRRNQEK"
BASE COUNT         2379 a         1164 c         1899 g         1606 t
ORIGIN      
        1 cctttcttaa atgtttaggt ttggtagctt aaagcataac acagaaaagg tatttccgct
       61 ttttaattat tcagttgtaa cacatgtatt gtaatcctac acggaatgaa tacttggaac
      121 ggcggtttcg gttgacattc tgccggattt tgggtaaaat cacagatgca aacaggcgag
      181 gaaagagaat agtatatgtg tggaatgcat cagagagaag gcggttggtg aaagcctttg
      241 caggaacaca ttgaaccggc tccggagctc tgtatgaaaa tacagcgtat atctgcgtta
      301 aagataaaag agagaacaac ggcaggcagg acgtctgcgg tgttaataag ggtggtaccg
      361 ccgagatgct cggtcccttg tggggaagag cgtcccggcg tttttatata ggcgcatata
      421 ggaaatgaga cgcttttccg gcagagaagt tttggcgcta tccgggaatc tgccgcgtta
      481 ccggaaattt ttacgatccg ggagaaacgg gcaaaacgga agaaacggtc gaaacggtcg
      541 aaacggtcga ttcggacgga tatggctaaa ctgctgcgct atggaagcga tggaagaaaa
      601 gtcggaacaa aagataaaaa gttccgaaaa tgatttggga aatgaggtaa gaaaatggcg
      661 aaagaaaaga aacttgtaca gtcgatcaca tcgagagacg aagattttgc acagtggtat
      721 acagatgttg tgcgggaagc aaaattatgt gattattcag gcgtaaaggg atgtctgaac
      781 tatcttccga acggatatgc aatatgggaa aatattcagt cggatcttga taaacgtttt
      841 aaagacacag gtgtggaaaa cgtatatctt ccggttttga tcccggaaag ccttttgcag
      901 aaagaaaaag accacatcga agggttcgcg cctgaagtag cgtgggtaac acacggcgga
      961 gcggagccgc ttcaggagag attctgtatc cgtccgacat ctgagacatt gttctgtgac
     1021 gtgtggagca agacggttca gtcttacaga gaccttccga aagtatggaa tcagtggtgc
     1081 tcggttcttc gttgggagaa aacgacaaga ccgttccttc gttcaaggga attcctctgg
     1141 caggaaggtc atacgattca tgcgacatat gaagaagcgg aagaacgtac aaaattaatg
     1201 tggaaagtat ataaagactt tgtagaagaa aatcttgcga ttccggttgt tgccggaaga
     1261 aaaacagaaa gtgaaaaatt tgcgggagcg caggacactt atacgattga agcactgatg
     1321 cacgacggtc aggctttaca gtcggcgaca agccatttct tcggaaattc tttcccggat
     1381 gcatttgaca ttaagtatgc agataaaaat aatgaactgc acagtgtgta cgagacatca
     1441 tggggattat ccacccgaat catcggtgcg atcatcatgg ttcacggcga tgacagcggt
     1501 cttgtacttc cgccgagaat cgctccggta cagacaagag ttattccgat cgcgcagcat
     1561 aaagaaggtg ttctcgataa ggcaaatgaa cttcttgacg ctcttaataa agccggatat
     1621 cgcgcgaaga tcgatgattc cgaaaagagt ccgggatgga aattcagtga gcaggagatg
     1681 ctcggaatcc cgactcgtat cgagatcgga ccaaaagata tcgaaaacgg tcaggttgtt
     1741 gttgtgcgca gagatacaag agagaagatc gttgtggcaa tcgatgagat tacaacgaaa
     1801 ctcggtgaaa tccttgagac gatccaggca gacctttatg cgaaagcaaa agcgttcctt
     1861 gatgaccaca tcagttctgc ggtgactatg gatgagatga aagatgcggt ttccgaaaag
     1921 aaaggtttcg taaaggcaat gtggtgtgga gacgaagctt gtgaggatga gattaaagca
     1981 cagacaggcg gagtgacatc acgatgcatt cctatggaag aagaacatct gtctgatgta
     2041 tgcgtctgct gtggaaaacc tgcgaaacat atggtttact ggggaagagc atactaaaat
     2101 acattatttg taaatgggca gtttaaaaca ggagtggatt tcttcgcttt tggaagaaat
     2161 acgttctgtt ttacagcgct gcttctcgat aaataatata gaagaaataa gatagtgttg
     2221 tacaaaaagg ctataatcgt tttggaaaat gaacgactat agcctttttt gaaatcctaa
     2281 atatgcaaaa tgacgaaaat ggggggatga taatgacaat tttgtttaaa tgtgataagg
     2341 ttataaggtc tatgaaaaat aaaattgtga aaaaagggga aaggaaaagt atgaaaaaga
     2401 gaaagtatct ggcattgtta ttagctgccg gaatgatcgc acagacagct ctttcgaccg
     2461 gcggagttgt gcaggttcag gctgccgaag acacatacgt taaaacagat gagctgacag
     2521 acaacgatac ggcggcaccg aagaaggata gtgttatacc gtcaaagaat cagtatgagt
     2581 atcagaaaca ggaactggcg gctttttgtc attttggaat gaatacatat accggaagcg
     2641 agtgggggaa cggaaaagaa aatccgaatc agtttcagct gacaaatgat tttgatgaag
     2701 aaacttatgt aaaagcgatt catgacgcag ggtttaagaa actgatcatt acggcaaagc
     2761 atcatgacgg tttctgcata tggccaagtg aaatgacgac acatgatacc gaaacggcag
     2821 gttatgaagg cgacgtttta gaagaacttt ccagagcgtg tacaaaatat aatattgaca
     2881 tgggactgta tctttccccg tgggatgtaa atagtgaata ttatggttac aaagataagg
     2941 atggaaaaaa acttgtcgga gctgacggac agccgttgaa tggaaaaaca tgggatcagg
     3001 taaaacaaga agacgctctt gattataacg aatactatga taatcagttg aaagagattc
     3061 ttggaaatga taagtacgga aacaacggtc attttaaaga agtttggatg gacggcgcaa
     3121 aagatacgaa cgacaagagc aatgcgcagg attatgattt ccagagatgg tttaagacaa
     3181 ttcagcaata cgagggaaaa gcaagcggaa agtacgagga tgattgcctt cttttcggcg
     3241 cggaatctta tacaactgta agatggatcg gaaatgagag aggatatgcg gctgaagaaa
     3301 cgtgggcaaa gtcaacaatc gacagagaga ataatacgat caatagtaac agtagagacg
     3361 gatatacgaa aggatttccg aatggaaatc agtggactgt gccggaggcg gatacaaaga
     3421 ttacgtcagg ctggttctgg ggagaaagca agaaaacgcc tctttcgatg gagcagcttg
     3481 caggtattta tttcggatcg gttggacaca acgctacatt gttactgaat gttcccccga
     3541 ataaacaagg aacggtagat gcggatattc ttgctcgtgt ggcagagttc ggaaaagcag
     3601 ttcaaaatac gtttgataag aacttggctg aaaaagcgtc tgtttctgcg acagaagtaa
     3661 gaggaaactc aaagaaatat tcaccggaga atctgctcga cggaaatgat gagacttatt
     3721 ggacagtagg agacggtacg acaagcggaa aagtattgat cgatttggga gaatccaaaa
     3781 agttcgatgt tgtgtcgata gaagaggcaa tccagtttgg acagcgtatc ggatctttca
     3841 aagtagaata taaaaacggt aatggagaat ggaagacgtt tgaccaggga acaacgatcg
     3901 gagcaaaacg tctctgtcgt aaaaaagctg taaaagcgga taagcttcgt attacagtta
     3961 ctgctcataa ccaggcggaa aataaagttc cgatattaag cgagattggc gtatatgagg
     4021 ctgcggaagg atttgaactt ggaacgggta tcccgtcagg tctgcagacg aaagatgacc
     4081 gtggatttac gcttagcagc ggatggcatc aggaaacgaa cgatcagatg atagaaggaa
     4141 cgggtatctg gatcaacgga aacggaaatg gggcgaatgc gccatatgcg gagacgaagt
     4201 ttaaaggtac gaaggcatgg gttatcggta cgatcgatca aaaacacggt ccggcagatg
     4261 tatatattga cgggaagaaa gttgcatcga ttaatacata ttctgcaaca agaaagttgg
     4321 gacagatttt atatgaaacg aatacgttag aagacaaaga gcatactctt aagattgtaa
     4381 atacagggtc taatactcag gctgtaggat tagatgcagt tgcgtatctt gataacggtg
     4441 gaaagggaat ggtagaactt gaaaaagatg cctatcgagt gaatgaagat acaaaatacc
     4501 caattaagct gaaacgcgtc ggaggtacga acggtgaatt gacagtgcag tttgaggttg
     4561 ctccgggaag cgcatatcag aagcattttg atgctgatgg aaatatgaca gtaacattta
     4621 aggatggaca ggaagaggca gaagcatttg tcacaacgaa gcgtgtgaag gaaaaagaag
     4681 gtgacgttta tttttctgct tatttaagcg ctccgacaga agatgttctt ctgggattca
     4741 ttacaaaagc aaagattacg attgcagata cagaatctta tgacaagcag gaacttcttg
     4801 acaagatcaa agaaacagag aaagcgggat atcaggaatc actttataca acagcaagtt
     4861 ataaaaatta tgtagatgca ttaaaagcag ccaaagaagc ggcaaagaaa gcaaacttga
     4921 ctcaggaaga agctgcaaaa gcatgtgcgg ctcttgatgc ggcaatcgca agtttgacaa
     4981 aacgaagtac attcagtgaa acagacagac tgcagttccc taaagtaaaa ggacgtagta
     5041 cgactgtaga agcggaatat tttgtattag attcgtctaa agctgaaaaa ggtaaagagg
     5101 ttcgcattca gccggataca ggtgcaagta atggggcaaa agtaggatgg tttgaaaaag
     5161 gtaatgtgat aaaagcgcca ttccatgctg aaaaagaagg aaaatataca gtaactgtga
     5221 cataccaaag tggaagagat tctggcaatc cgaataaaat taactggggt ggaacgaatg
     5281 tagacagcgg ttcggtcaat gtagtaggaa agaatacaca gaatcctgtt tttgaagaga
     5341 agtcatttga cattaatgtg acaaaagcag gtgatggtga gttagtgctt actgcagatg
     5401 caagttcgtc tcctaatatt gacaagtttg ttatcacagc gaaagaagtt tctgctgcaa
     5461 catataagat tacggcgaca gcaggagagc atggtaagat tgaaggcgtt acaggtacgg
     5521 aagtgacagt agaagaaggc gcgagccata cgttcaagtt tgtaccggat gaaaattatg
     5581 cggtaaaaga tgttatcgta gacggcaact cgattggcgc ggtagattct tatacatttg
     5641 atgatgtgac ggcaaatggc catacgattg aagtacagtt tgaaaaggca gtttatgcag
     5701 aagataaccg atttgtattc ccaacggatg gtaatacaaa aacagctgag gcagagagat
     5761 ttgagattca tgatgtaaaa gatccgacag atggtgaatg gcattgcgga gtaactgcga
     5821 aagactgggc aagtggcgga aagattgtaa atgcgatgaa taaaggggat acgattaccc
     5881 tttactatga tgcaccgcag acaggcgaat atacagtgac ggtatattat agaagcggaa
     5941 atccgcagaa tggtcttaca tgggctgaaa aagatggaaa aatcaaatca ggaaatgtga
     6001 cagcaggagc aggagatagt gcagcggcaa cacatacgac agagtttaca ctgaatgtag
     6061 agacggcagg tccgggatgt cttgtattca cagcaccgga tgcgaaagct ccgcagttgg
     6121 ataaattcga tatcaagagt ccgggaacac cggttcctcc gatttctgca gtagagcttg
     6181 aagcggcagt tgaggctgct aagggattag atctgaatga ttacaaagat gacgatgcca
     6241 aaactgcatt tactgcaaaa ctggcagagg cggaaaaact tcttgcagac attgccggcg
     6301 gaagctttgc gggaacacag gacgaggtga atcagatggc aaaagagctg aaagctgcac
     6361 aggatgcttt gaatgaaaaa gagcagactc ctcaggtgaa taaagatacg cttaaggagg
     6421 caatcggcaa ggcggaagcg cttgatctga cacagtataa agaagaaggt aaggcagagt
     6481 ttaatgcggc tcttcaaaat gcaaaggctg ttcttgaaaa agcagatgca gatcaggatg
     6541 aaatcgatca ggcaacgctt gcattgaata cggcaatcca gaatctgaaa ccggtcgacg
     6601 gcggaagcgg cgatggaaac ggtgaaggaa acggaaatgg aagcacaggc ggaaacggaa
     6661 acggcagtgg aagcacaggc ggaagcggaa acggtagtgg aaacacaggc ggaaatggaa
     6721 gtacaggagg cagcggcaca ggaaattcgg cagtgcagac aggcgacagc tcaaacctgt
     6781 ttgtatatgc gggaacatta ctccttgcag cggcagcgtt cgtattcgga ttcagaagaa
     6841 gaaatcagga aaaataattc tgatgccgaa ggcattcgga cagtctgaat aaagagataa
     6901 cagagacggg aaagttatgg attatctgag aagataaccg tgattttccc gttttttgta
     6961 tcaggaaaac cacgcgatag tcgcgtggtt ccgtgaaagc tttagcggtg tattcagaaa
     7021 taaaaactcc taatgtgtat aataaaaa
//
LOCUS       DBMDXF010000095         6899 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2813_length_6899_cov_1.898205, whole genome shotgun sequence.
ACCESSION   DBMDXF010000095 DBMDXF010000000
VERSION     DBMDXF010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2813_length_6899_cov_1.898205"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(208..1545)
                     /locus_tag="ACZPD4_05040"
     CDS             complement(208..1545)
                     /locus_tag="ACZPD4_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1378473.1"
                     /translation="MEKMNAVNLEKMYEVLKFVEYGGYCRRSMDCVHGMLLIDRMKNE
                     AVVDKAVLFGWFRKMAVCAEQYERCGEGQNYKYLNPYGIVLSDEGEVLFLDTESRENA
                     EVMKQMQKRAVRSHFIRPVYEMDTCGSREPDLFGYGNTLRFMLAYMKVVPALTKREEI
                     RLFRICGKCIGETRKKYSSFLQVLKELPTVRNKTEKTDKKRVGVWSGIAAGAAGCILA
                     AVYMGDAHTDLAVSNGEKITGEDIKTDTGTDIETIETDVETTKTGSETTELGVEMTET
                     DMGKDKRANREAEVRSDRDGDTENEERKKDTADADEVKKNVTDESGKTDAAAEWMELY
                     LDQHTDSGNQKAIEIGEKARLSVLWKLADAYERSGMKTEQIQTYGQLIEWEQDPIQLE
                     EAGVRKMKLEAAQGDYKQAAETGKKVLERIEASEKITKMLKGYELYKGEEGES"
     gene            complement(1784..2884)
                     /gene="glf"
                     /locus_tag="ACZPD4_05045"
     CDS             complement(1784..2884)
                     /gene="glf"
                     /locus_tag="ACZPD4_05045"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1378474.1"
                     /translation="MKKYDYLIVGAGLYGAVMAYELGKKGKSCLVIDKRDHIAGNIYC
                     EDVEGIHVHKYGAHIFHTSDKKIWDYINQFAEFNHYINSPVAVYKDELYNLPFNMNTF
                     SRMWGIKTPEEAKKIIERQRKESGITEPKNLEEQALFLGGKDIYEKLIKGYTEKQWGR
                     KCTELPAFIIKRLPFRFVYDNNYFNDRYQGIPIGGYTPIVEKMLEKADVLTGTDFFEF
                     RAQNADIADKIIFTGMIDEYFGYRLGALEYRSVRFETETLDCDNYQGNAVVNYTDGEV
                     PYTRVIEHKHFEFGKQEKTVISREYSSEWAVGMEPYYPVNDEKNNALYEQYKKLADEE
                     ENVIFGGRLGNYKYYDMDKVIAAALEQLDRIV"
     gene            3119..4351
                     /locus_tag="ACZPD4_05050"
     CDS             3119..4351
                     /locus_tag="ACZPD4_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1378475.1"
                     /translation="MKGNVIVGQSGGPTAAINSSLAGVYRTAKDRGANKVYGMLFGIQ
                     GLLQERYIDLSEYITNDLGTELLKRTPAAFLGSCRYKLPEIHDNKDVYEKIFSILEKL
                     DIEAFIYIGGNDSMDTIKKLSDYAIVTGYRTRFIGCPKTIDNDLALTDHTPGYGSAAK
                     YIGTSVKEIIRDSFCLEYGKGLVSIVEIMGRNAGWLTGAAALAKGEDCLGPDLIYLPE
                     LPFDIEAFSEKVKDLLAKKPSVVVAVSEGIRLADGRYVCELGQSVDFVDAFGHKQLSG
                     TANYLASYIAGEFGCKTRAIELSTLQRSASHCASRVDILEAYQVGGAAVKAADEGDSG
                     KMVVLQRLSDDPYQCGTEVKDVHKIANDEKVVPREWVTEDGSYVTDDFVSYVRPLIQG
                     DVSPVMVDGIPRHLYRKN"
     gene            4602..5867
                     /locus_tag="ACZPD4_05055"
     CDS             4602..5867
                     /locus_tag="ACZPD4_05055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1378476.1"
                     /translation="MLIKNGFLIDPATKKSGNYDIRIKNGIITEIGNTLSPAPNEQVT
                     DAAGLTVAPGLIDTHIHFRDPGFTYKENIHTGSQAAAAGGFTSVICMANTSPVVDNTE
                     TLTDILERAKDEKIHVYQAASVSHKLRGEEMTDMEALKTAGACGFTDDGIPLKNAAFL
                     YEAMEKSKQLNVPISLHEEDPSFIKNNGVNHGAVSEQLGIYGSPSIAEESLVARDCMI
                     ALHSGADVVMQHISSGRSVDIIRHFKSLGANIHTEATPHHFTLTDEAVLTYGTLAKMN
                     PPLRSEADRQAIIAGLADGTIDIIATDHAPHSKEEKERSITAAPSGIIGLETSLALGI
                     TSLVRKGHLSLMQLLEKMTINPARLYHLDGGKIEKGAPADLVIFDPEAKWIPEFFASM
                     SSNSPFIGWELYGKVKMTVCGGEIVYTAI"
     gene            6652..>6899
                     /locus_tag="ACZPD4_05060"
     CDS             6652..>6899
                     /locus_tag="ACZPD4_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378477.1"
                     /translation="MKNFFKKTTLIPILFAMLIYSPLITSHATHLETQTKRQSLQKGS
                     VVQDQHVFAIPDNGWNVVDIELDYYESYYSSTNTQNAFP"
BASE COUNT         1913 a         1667 c         1202 g         2117 t
ORIGIN      
        1 tgttcattta ttattttaaa ttctttcatt ttctcctcac gttttcttca tttatatcgc
       61 atatactaag cttaccgaat taaaggtgat tacaagttgt ttttgcataa actttttcat
      121 aatacatgcc gggaatcata ttgattaccc ggcatcctcc cttttcagaa ctatttttct
      181 ccatatttcg tattttcact ctgacgctta cgattctcct tcttcgcctt tatataactc
      241 atacccttta agcatctttg tgattttttc cgacgcctca atccgttcca aaactttctt
      301 cccggtctcc gccgcttgtt tataatcgcc ctgcgctgct tccagcttca tttttcttac
      361 gccggcctct tctaattgta tcggatcttg ctcccattcg atcagttgac cgtatgtttg
      421 aatctgctcc gtcttcattc ccgaccgctc ataagcatcg gccagtttcc acaaaacgga
      481 caatctcgct ttttccccta tctctatcgc cttttgattt ccactatccg tatgttgatc
      541 caaatacaat tccatccatt ccgcagcggc atctgttttt ccactctcgt ctgtcacatt
      601 tttcttcact tcatccgcat ctgccgtatc tttttttctt tcctcgtttt ctgtatctcc
      661 gtctctatct gaccgcacct ccgcttctct gtttgctctt ttatctttgc ccatatctgt
      721 ttctgtcatt tccacaccta gttctgtcgt ttctgagcct gtttttgtcg tttctacatc
      781 tgtttctatt gtttctatat ctgttcccgt atctgttttt atatcctccc ctgtaatctt
      841 ttctccgttc gatacagcca gatctgtgtg agcgtctccc atataaaccg ccgccaaaat
      901 acatcccgcc gcacctgccg cgattcccga ccatactccg acacgctttt tgtctgtctt
      961 ttctgttttg ttcctcactg taggaagttc ttttaaaact tgcagaaacg agctgtattt
     1021 ttttcttgtc tctccgatgc atttcccaca tatacgaaaa agacggattt cttcccgttt
     1081 tgtcagcgcc ggaactactt tcatatatgc cagcataaaa cgaagcgtat tcccgtatcc
     1141 aaacagatca ggctctctgc tgccgcaagt atccatttcg tataccggtc tgataaaatg
     1201 acttctgaca gctcttttct gcatctgctt catcacctcg gcattttctc tcgattctgt
     1261 atccagaaac aacacttctc cttcatcaga caacacaatt ccatacggat tcaaatactt
     1321 ataattctgt ccttcaccgc atcgctcata ctgctctgca cagactgcca tttttcggaa
     1381 ccatccaaat aaaactgctt tatcgacgac cgcttcattc ttcatacgat cgataagcag
     1441 cataccatgt acacaatcca tgcttctgcg gcagtaacct ccgtattcga caaatttcaa
     1501 tacttcatac attttttcca agttcactgc attcattttt tccatattca ctttttcatc
     1561 gggtgcacat aaccggaagc ggggcgtccc tcctgccccg ttcccgaatg agcttattat
     1621 tcttggcgaa atccggcgaa acaactgccg taaaagatgt gtcttgaatc agactcattt
     1681 aataagtatt tgtatcttac ttgaatttat acagaaaagc aagtgtctgc attatctttt
     1741 ttcttttgct tttttgcctt tttcttttgt ctctttctta cgtctacacg atccggtcaa
     1801 gctgttcaag agccgctgcg atcactttat ccatatcata gtatttataa ttcccgagtc
     1861 ttccgccgaa aatcacgttt tcctcttcgt ccgccagttt tttatactgt tcatataaag
     1921 cgttgttttt ttcgtcgttg accggataat aaggctccat tccaactgcc cactccgagg
     1981 agtattctcg tgaaatcaca gttttctcct gctttccgaa ctcaaaatgc ttatgttcga
     2041 tcacacgtgt ataaggcact tcaccatcgg tataattgac aaccgcattc ccctgataat
     2101 tgtcacagtc aagtgtctcc gtctcaaatc tcaccgaacg atactcaagc gctccgagtc
     2161 tgtaaccaaa atattcatcg atcatccccg taaatatgat cttgtctgca atatccgcat
     2221 tctgagcacg aaattcaaaa aagtctgttc ccgtaagaac atccgctttt tcaagcattt
     2281 tttccacgat cggagtatag ccgccgatcg ggattccctg atatctgtcg ttgaaataat
     2341 tgttgtcata cacaaaacga aacggaagcc tcttaatgat aaatgccgga agttccgtac
     2401 attttcttcc ccactgcttc tctgtatatc ccttgatcag tttttcgtaa atatccttcc
     2461 caccgaggaa aagcgcctgc tcctcaaggt ttttcggttc tgttatcccc gactcttttc
     2521 gttgtctttc gatgatcttc tttgcctctt ccggtgtttt tattccccac atcctgctga
     2581 atgtgttcat attaaacggc agattataca actcgtcttt ataaactgct accggagaat
     2641 taatatagtg attaaactcc gcaaattgat ttatataatc ccatattttt ttatcagacg
     2701 tatggaaaat atgcgctccg tacttatgca catgaatccc ttctacatct tcacagtaaa
     2761 tatttccggc aatgtgatct cgtttatcga tcacaagaca tgactttccc ttttttccaa
     2821 gctcatatgc cataactgcg ccgtataatc ccgcaccgac gatcagataa tcatattttt
     2881 tcataccaag cctctctttc cgcgccgcaa aatatcagcg ctgatttatc ctgtatttta
     2941 acagcataac atcttttccg caatttttct tataatgtat cactttttgt cattttcgtt
     3001 attttgccta tgtttttgat ttttttgttt acaaattagt cattatgcac taaaataatt
     3061 gtatatccgt ccgcagcaac aacctgcggg caaacataca gaacggaggt acttcatcat
     3121 gaaaggaaat gtaattgtcg gacaatccgg tggtccgaca gcggcaatca actcaagtct
     3181 cgcgggagta taccgcactg ccaaagacag aggcgcaaac aaagtatacg gtatgctttt
     3241 cggcattcag ggactccttc aagagcgtta catcgatttg tccgagtaca ttacgaacga
     3301 tcttggtaca gaacttttga aaagaacccc tgcggctttc ctcggatcat gccgctataa
     3361 actgcctgag atccacgata ataaagacgt atacgaaaaa atcttttcaa ttctcgaaaa
     3421 gcttgacatt gaagccttta tttacatcgg cggaaacgac tcaatggata cgatcaaaaa
     3481 actttctgat tacgccattg taacaggtta ccgcacccga tttatcggat gccctaagac
     3541 gatcgacaac gatcttgctc ttacagacca cactccggga tacggaagcg ccgcaaaata
     3601 tatcggaaca tctgtaaaag aaatcatccg cgacagcttc tgccttgaat atggaaaagg
     3661 gcttgtttct atcgttgaga tcatgggaag aaacgccgga tggctgaccg gagcggcagc
     3721 tcttgcaaag ggcgaggact gtttgggccc agatcttatc tatctgcctg aacttccttt
     3781 tgatatcgaa gctttcagcg aaaaagtaaa agatcttctg gcgaaaaaac cgtcggttgt
     3841 tgtcgccgtt tcggagggaa tccgtctcgc cgacggaaga tatgtatgcg aactcggaca
     3901 aagcgttgat tttgtcgatg cgttcggtca caaacagctt tcgggaactg caaactatct
     3961 tgcaagctat atcgccggcg aattcggctg caaaacccgc gcgatcgaat tgagcacgtt
     4021 gcagcgctcc gcttcccact gcgcttccag agtcgatatt ctcgaagctt atcaggtagg
     4081 cggcgcggca gtaaaagctg cggatgaagg agattccggc aagatggtcg ttctccagcg
     4141 tctttccgac gatccgtatc aatgcggaac agaagtaaaa gatgtgcata agattgcaaa
     4201 cgacgaaaaa gtcgttccgc gcgaatgggt tacagaagac ggatcttatg taaccgacga
     4261 ttttgtttca tatgtacgcc cgctcattca aggtgatgta tctccggtaa tggttgacgg
     4321 tatcccgcgt cacctctacc gcaaaaatta attcacatac tgccgctcct gatatctatg
     4381 atatgttcac gatcaggatg cggcagtttt ttatatccgg ttttataaca ctgctccttt
     4441 ttattctctt gtctctttct tattcttcct ttatacttta cctatataca gatgcgtttt
     4501 tcacttatat atgattacat atgaatccat ttatttccgt tacgcgtctt tgtttcgcat
     4561 aataattatt acagcaatct tatattcaag gaggtttttt catgcttatt aaaaacggtt
     4621 ttcttataga ccctgccact aaaaaatctg gaaattacga tatccgaatc aaaaacggga
     4681 tcattacaga aatcggaaac actctttcac ctgccccaaa cgagcaggta acagatgccg
     4741 ccggtctgac tgtcgcgccg ggacttatcg atacgcatat ccatttccgc gaccccggat
     4801 ttacttacaa agaaaatatc cacacaggtt cacaggccgc cgccgcagga ggatttactt
     4861 ctgtcatctg tatggcaaat acgtctccgg tcgtagataa tacagaaact ttaacggata
     4921 ttttagaaag agcgaaagac gaaaaaatac acgtttatca ggccgcttcc gtctctcata
     4981 aactgcgcgg cgaagaaatg accgacatgg aagcgttgaa aactgccgga gcatgcggat
     5041 ttaccgacga tggcattccg ttgaaaaacg ccgcgttttt atacgaggca atggaaaaat
     5101 ccaaacagct aaacgtaccg atcagccttc atgaagaaga tccgtcattt attaaaaaca
     5161 acggcgtcaa ccacggcgcg gtgtctgaac agctcggcat ttatggttct ccctctatcg
     5221 ccgaagaatc cctcgtcgca agagactgca tgatcgcgct gcattcaggc gccgacgttg
     5281 ttatgcagca tatcagctcg ggacgttccg tagacattat ccgccacttt aaatcactcg
     5341 gcgcaaatat tcacacggaa gcaacgccgc accatttcac actgacagac gaggctgttc
     5401 tgacatatgg aacacttgca aagatgaacc cgccgcttcg ctccgaagct gaccgtcagg
     5461 caatcatcgc cggactcgcc gacggaacga tcgatattat cgcaacagac catgccccgc
     5521 acagcaaaga agaaaaagaa cgctccatca ctgccgctcc aagcggaatc atcggtttgg
     5581 aaacgtcact ggcgctcggg atcacttctc ttgtacgcaa agggcatctg tcacttatgc
     5641 agcttctcga aaaaatgacg atcaatcccg ccagactgta tcatttagac ggcggaaaaa
     5701 tcgaaaaggg cgctcctgcc gacctcgtga tcttcgatcc cgaagcaaaa tggattcctg
     5761 aattcttcgc ttccatgtcg tcaaattcac cgtttatcgg ctgggaattg tacggaaaag
     5821 tgaaaatgac tgtatgcggc ggagaaattg tatacactgc catttaatta cttttaaaaa
     5881 tctccatcct tcaagtaaga atggagattt tttatataat actataaaga ctccttgacc
     5941 aaaagaaaag cgatgacaaa catctaaaac atgtaagtta ttaccataac ctatgcattt
     6001 tgttacattt caaaaaaaag agtaaaaata gtgttataat taaggagaaa aaatatcatc
     6061 tattcaaact ctcaaataaa ttgacagcaa cacaaagtca atcaccctaa ccaaaaatat
     6121 ttttaactaa tcgttatgtt gcctcaagaa atatatcata aggagtgata ccattggaat
     6181 ttttatttag tttgccataa aaggttataa gttttaaatt cctcttaata aaaggcgaaa
     6241 aaatggagtc aattataaaa aagtcggtcg gttctttcaa tcacataaag tagaaactga
     6301 ttcccctatt tttgcaaagg aaaaatcaga agaatttatt gcgaaagata atattgaaca
     6361 aatcactgat ttttacacat tagcgggtca tacagaatct gcagcccgtg aaaaagcaat
     6421 cacatatata tcggaacgtg atgatactta tcaaaaagca atcgcaaaag tttatgatgt
     6481 aactgacgaa aagttttggg catatttaga gaaattaaaa aactataaac aaagcaacct
     6541 attagaatta aaaaagaatt ctataaagag ttatgccaat gaaaacttta cttttagttt
     6601 tcattaaatt cactactttt aaatatttga tgtacgagga agggaacaga tatgaaaaat
     6661 ttttttaaaa aaactacatt aattccaatt ttatttgcta tgttaattta ctctccactc
     6721 atcacaagcc atgcaacgca cttagaaact caaaccaaac gtcagtcatt gcaaaaagga
     6781 agcgtagttc aagatcaaca tgtttttgca atacccgata atggatggaa tgtagtggac
     6841 atagaattag actattacga atcttattat tcttccacta atacacagaa tgcatttcc
//
LOCUS       DBMDXF010000096         6878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2828_length_6878_cov_1.468792, whole genome shotgun sequence.
ACCESSION   DBMDXF010000096 DBMDXF010000000
VERSION     DBMDXF010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_6878_cov_1.468792"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="ACZPD4_05065"
     CDS             <1..435
                     /locus_tag="ACZPD4_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR1378478.1"
                     /translation="EGEIYYTTDGTDPTAKTGKKYKEPILLEKEGTTEIRAIVVNMKG
                     IPSGVISQTYTIEFPIADAPAVTPSTGRYTDPQKITITVPDGYTAYYTTDGSEPTASS
                     EKYTGPIDMPANTTTTFNAVLINNRNGKATEVTTRSYITTAE"
     gene            583..1764
                     /locus_tag="ACZPD4_05070"
     CDS             583..1764
                     /locus_tag="ACZPD4_05070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1378479.1"
                     /translation="MSRFCLPRDIYHGKGCLEELKNLKGKKAILVVGGGSMKRQGFLD
                     RAVGYLKEAGMEVQLFEGVEPDPSVETVMKGAEAMRAFEPDWIVSMGGGSPIDAAKAM
                     WAFYEYPDTTFEELCTPFNFPELRQKAKFCAIPSTSGTATEVTAFSVITNYQTGVKYP
                     LADFNITPDVAIVDPDLVAGLPVKQVAYTGMDALTHAIEAYVSTLNGPFTDPLALQAI
                     EMVLDYLPASYNCNMDAREQMHYAQCLAGMAFSNALLGIVHSMAHKTGAAFSTGHIPH
                     GCANAIYLPYVIQYNAKDSVAAKRYAEIARRMGLEGNSEKALINSLVAKIDEFNAELN
                     IPKTLKDFGINEEEFKEKIAKISELAVGDACTGSNPRAIDPAAMEKLFTCTYYGTEVD
                     F"
     gene            2004..2891
                     /locus_tag="ACZPD4_05075"
     CDS             2004..2891
                     /locus_tag="ACZPD4_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR1378480.1"
                     /translation="MYKIMKAEKLADKIFLMDVHAPRVASHCEPGQFVIVKMDEKGER
                     IPLTICDYDREAGTITIVVQEVGASTTKMGTLKEGDYFRDFTGPLGCASEFVHEDLET
                     LKNKKMLFVAGGVGAAPVYPQVKWLKEHGIEADVIVGAKTKDMLILEDEMKAVAGNYY
                     PCTDDGTYGHAGMVTTMIEKLVNEGKKYDVCVAIGPMIMMKFVCILTKKLELPTIVSM
                     NPIMVDGTGMCGACRLQVGDEIKFACVDGPEFDGHLVDFDQAMKRSQMYKTAEGRALL
                     KLQEGDTHHGGCGHCGGDE"
     gene            2891..4285
                     /gene="gltA"
                     /locus_tag="ACZPD4_05080"
     CDS             2891..4285
                     /gene="gltA"
                     /locus_tag="ACZPD4_05080"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR1378481.1"
                     /translation="MADVLKKVPVREQDAKVRATNFEEVCLGYNMEEAMEEASRCLNC
                     KNAKCIQGCPVSIDIPAFIQEVKEGNIEEAYKVIGKSSALPAICGRVCPQESQCEGKC
                     IRGIKGEPVSIGKLERFVADYALDHDIKPVGAEEKNGHKVAVIGSGPSGLTCAGDLAK
                     LGYDVTVFEALHELGGVLVYGIPEFRLPKQKIVAKEIEKVKELGVKFETNVVIGKSTT
                     IDQLMEEEGFEAVFIGSGAGLPMFMGIPGENANEVFSANEYLTRSNLMKAFDDSYDTP
                     IAAGKKVAVVGGGNVAMDAARTALRLGAEVHIVYRRSEAELPARVEEVHHAKEEGVIF
                     DLLTNPKKINVDDNGNITSMTVVKMELGEPDASGRRRPVEIEGSEYDIEVDTVIMSLG
                     TSPNPLISSTTKGLDVNRRKCIVADEENGQTSKEGVFAGGDAVTGAATVILAMGAGKA
                     GAKGIDEYLKNKEK"
     gene            4426..5139
                     /locus_tag="ACZPD4_05085"
     CDS             4426..5139
                     /locus_tag="ACZPD4_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR1378482.1"
                     /translation="MIAEEKKIWKIREQLEDLTEENYKQFNEKLLPGVKHIIGVRLPA
                     LRKIAKETAKSNYSEYLNEAGTMLGEASYHEEFMVWGLVIAYIKADRSEVERHLDEFV
                     PKINNWAVCDSCVTSYKFMQKESDFWFDYVKKYAESKNEFELRFMIVSLMSHFLDKEH
                     IDEILTICGKIDHDGYYVKMGNAWALSVCYVKFPEKTRAFLEKDTLDDFTHNKAIQKI
                     RESYRVSKEEKEQLTCLKR"
     gene            5176..>6878
                     /locus_tag="ACZPD4_05090"
     CDS             5176..>6878
                     /locus_tag="ACZPD4_05090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HelD family protein"
                     /protein_id="HIR1378483.1"
                     /translation="MEADNRQFDGKLFLKDVNRKLNDRLAEIQKDLNQGLKEIENMHE
                     YYWENYTEMDQYGYEDYDNQQALLQQVNANRDKSKLMKRLEKMIDSPFFGRVDFLFEA
                     EEEEEPFYIGIGNFGERAGMTPLIYDWRAPVSSLFYDFDKGEAFYEAPSGVIKGEVVS
                     KWQYKIRGGKMIYGFESDVKIDDEILKHELGNNGDVKLKNIVRTIQKEQNAIIRNTKD
                     KILVIQGAAGSGKTSIALHRIAYLLYHDRKNLKSSNILILSPNSVFADYISHILPELG
                     EENIKEMSFDLFAYKELQDTAADCEDRYDQLERMMKFDDRRAQERFEWKQSAGFVGEA
                     EGFFAVLEDELMEFREVEFRGMRFSEEEIIHLFYDKFYQIPLLKRMDAVMEYFIDSYE
                     TLKGRNIEEEDREFLQRKFDKMYVTKDIYAIYNKLLAVCGQEQMAELPYERRKIQYED
                     VFPMLYVKYRLTGKNEHKNIKHLVIDEMQDYSYLQYLILKELFDCKMTILGDRAQTLG
                     KEQQDVLKFLPKVFGKNIRTVILNKSYRNTREIADYAGKIANVKDIEFLDRHGKDVAE
                     R"
BASE COUNT         2307 a         1064 c         1739 g         1768 t
ORIGIN      
        1 gaaggtgaaa tctattatac gacggacgga acagatccga ctgcgaagac agggaaaaaa
       61 tataaagaac ctatcttact ggaaaaagaa ggaacaacgg agattcgcgc tattgttgta
      121 aatatgaaag ggattccgag cggagtgata tctcagacat atacgattga atttcccatt
      181 gcagatgccc ctgcggtgac accgtctacc ggccgatata cagatcctca gaagattacg
      241 atcacagtac ctgacggata tactgcttat tatacgacag acggatctga accgacagct
      301 tcatcggaaa aatatacagg accgatcgat atgccggcga atacaacgac tacatttaac
      361 gctgttctca tcaataaccg aaatggaaaa gcgacggaag tgacaacgcg aagctacatt
      421 acaacagcag aatagagtat gtgaaaaaca accgtgatat tatgtgaaag ttacatatgt
      481 tttatgcaat ttttacaaat tcagggttgt ttttttgaat taaaagagtt ataatattta
      541 tgttaagaaa ataacaatat tcaaataaag gagatgtcgg aaatgagcag attttgttta
      601 ccaagagaca tttatcatgg aaaaggatgt cttgaagagt tgaaaaacct gaaaggaaaa
      661 aaagcaatcc tcgtagtagg cggaggatct atgaaacgtc agggatttct tgacagagca
      721 gttggatatt taaaagaagc gggaatggaa gtgcagttgt ttgaaggcgt agaaccggat
      781 ccgtcagttg agacagttat gaaaggcgca gaagccatga gagcatttga gccggactgg
      841 attgtatcta tgggtggagg ctcaccgatt gatgcggcaa aggcaatgtg ggcattttat
      901 gagtacccgg atacaacatt tgaagaatta tgtacaccgt ttaactttcc ggagctgaga
      961 cagaaagcaa aattctgtgc aattccgtct acatcgggaa cagcgactga agttacggcg
     1021 ttttctgtca ttacaaacta tcagacaggc gtaaaatatc cgttggctga ctttaatatc
     1081 actccggatg ttgcgattgt cgatccggat cttgtcgcag gtcttccggt aaaacaggtt
     1141 gcatatacag gtatggatgc gctgacacat gcgattgaag catatgtttc cacattaaac
     1201 ggaccgttta cagatccatt ggcgctgcag gcgatcgaga tggtattgga ttatcttccg
     1261 gcatcatata actgcaatat ggatgcaaga gaacagatgc attacgcaca gtgtctggca
     1321 ggaatggcat tttcaaacgc acttcttgga atcgtacatt ctatggcaca taagacaggc
     1381 gcagctttct ccacaggtca tattccgcat ggatgtgcaa atgcgattta tcttccgtat
     1441 gttatccagt acaatgcaaa agattctgta gcggcaaaac gttatgcaga gatcgcgcgt
     1501 agaatgggac tggaaggaaa ttccgaaaag gctcttatta acagccttgt agctaaaatc
     1561 gatgaattca acgcagaact gaacataccg aaaacattga aagatttcgg tatcaatgaa
     1621 gaagaattca aagagaagat tgctaagatt tccgagcttg cggtaggaga tgcatgtaca
     1681 ggatctaacc cgagagcgat cgacccggca gctatggaga aactgttcac atgtacatat
     1741 tacggaacag aagttgactt ctaaaattta acacaaaaat acactggtaa ttgataaaca
     1801 ataaagtgat atcaaccttt taaatagtga ttttacacaa aaacggttgg caggtgtgaa
     1861 ctgtaaagtc gctgtcttga tagaagtatc gtgacagcgg ctttttgtaa tggagtcttt
     1921 tggcacttac ggagatttga acttgccata gagtggattt tggcgtatta tagttaagag
     1981 tgaaattttc aaggaggaag accatgtata agataatgaa agcagaaaaa ctggcagaca
     2041 agatcttttt gatggatgtg cacgcgccgc gtgttgcgag tcattgtgag ccgggtcagt
     2101 ttgtgattgt aaagatggat gagaaaggcg agcgtattcc tctgactatc tgtgactacg
     2161 acagagaagc ggggactatc acgatcgtcg ttcaggaagt gggcgcttct acgacaaaga
     2221 tgggaacttt gaaagaggga gattatttcc gtgattttac aggaccgctt gggtgcgctt
     2281 ctgagtttgt tcatgaagat ctcgaaactt taaaaaataa aaaaatgctg tttgtagcag
     2341 gcggtgtcgg agcggctccg gtgtatccgc aggtgaaatg gctcaaagag catggtatag
     2401 aagctgatgt gattgtcggt gctaagacaa aagatatgtt gattctggaa gatgagatga
     2461 aagcagttgc gggaaattat tacccgtgta cggacgacgg tacgtacgga catgccggaa
     2521 tggttacgac aatgatcgaa aaacttgtaa atgaaggcaa aaagtatgat gtctgtgttg
     2581 ccatcggacc gatgatcatg atgaaatttg tctgtatttt gacgaaaaaa cttgaacttc
     2641 ctacaatcgt cagcatgaat ccgatcatgg tagacggaac gggaatgtgc ggagcatgcc
     2701 gtcttcaggt tggagatgaa atcaaatttg catgtgtaga cggaccggag tttgacggac
     2761 atcttgtaga ttttgatcag gcgatgaaga gaagccagat gtataaaact gcagaagggc
     2821 gcgccttact gaagcttcag gaaggtgata cccatcatgg aggatgcgga cattgcggag
     2881 gtgacgagta atggcagatg tattgaaaaa agttcctgtc agagaacagg atgcaaaagt
     2941 acgagcaaca aattttgaag aagtttgtct tggttataat atggaagagg cgatggaaga
     3001 agcttccaga tgtttgaact gtaagaatgc aaagtgtatt cagggctgtc cggtttctat
     3061 tgatattcct gcatttattc aggaagtgaa agaaggaaac atcgaagaag catataaagt
     3121 gatcggaaaa tcaagtgcgc ttccggcaat ttgcggacga gtatgtccac aggaatcgca
     3181 gtgtgaagga aagtgtatcc gcggaattaa aggtgagccg gtttcaatcg gaaaacttga
     3241 aagatttgtt gcggattatg cgttggatca tgatattaaa cctgtcggtg cagaagagaa
     3301 gaacggacac aaagttgctg taatcggttc cgggccgtcg ggtcttacat gtgcgggaga
     3361 tcttgcaaag cttggatatg atgtgactgt atttgaggcg cttcatgagc tcggcggagt
     3421 gcttgtatac ggaattccgg agttccgtct gccaaaacag aagattgttg cgaaagagat
     3481 agagaaagta aaagaacttg gcgtaaaatt tgagacaaat gttgtgatcg gaaaatccac
     3541 aacgatcgat cagcttatgg aagaggaagg atttgaagcg gtctttattg ggtccggagc
     3601 aggacttccg atgtttatgg gaattccggg tgagaatgca aacgaagtat tctctgcaaa
     3661 tgaatacctt acgagaagta atctgatgaa agcatttgat gattcttacg atacacctat
     3721 tgctgccggc aagaaagttg ctgtagtagg cggaggaaat gtagcgatgg atgctgcaag
     3781 aacagcactt cgtcttggcg cagaagtaca tattgtgtac agaagaagcg aagcggaact
     3841 tccggcgaga gttgaggaag ttcaccatgc aaaggaagaa ggcgtaattt ttgatctttt
     3901 gacaaatccg aaaaagatta atgtagatga taacggaaat attacaagta tgacagttgt
     3961 aaagatggaa cttggagaac cggacgcttc cggaagaaga agacctgtag agattgaagg
     4021 ctcagagtac gatattgaag tcgatactgt aatcatgtct ttaggaactt caccgaatcc
     4081 gttaatttca tctacgacaa aaggtttgga tgtaaaccgc agaaagtgta tcgttgccga
     4141 tgaggaaaac ggacagacat caaaagaagg tgtgtttgca ggcggcgatg ctgttacagg
     4201 tgccgctacg gttatacttg caatgggagc aggaaaggcc ggagcaaaag gaattgatga
     4261 gtatttgaaa aataaagaaa agtaaaaagc aataaaaagc aataaaaagc aataaaaagg
     4321 ggctctcttt ccgggtttgg agagggagct ttcttaatac tttttaaatg aatggaaaag
     4381 taagcaaaaa agactttgca tttagtcggc attttggaga atcacatgat agcagaagag
     4441 aaaaaaatct ggaagataag ggaacaatta gaagatctta cagaagaaaa ttataaacaa
     4501 tttaatgaga aactccttcc cggagtaaaa catatcatag gagtgcgcct tccggcttta
     4561 agaaaaattg cgaaggaaac tgcaaaaagc aattacagcg aatatttgaa tgaggcaggg
     4621 acaatgcttg gagaggcttc atatcacgaa gagtttatgg tatgggggct tgttatcgct
     4681 tatataaaag cggatagaag tgaagtggaa aggcatttgg atgagtttgt tcctaaaatc
     4741 aataactggg cagtgtgcga cagctgtgtg acaagttata aatttatgca gaaagaatca
     4801 gatttttggt ttgactatgt gaaaaaatat gcagaaagta agaacgagtt tgaacttcgt
     4861 tttatgattg tttcacttat gtctcatttt ctggataagg aacatattga tgaaatactc
     4921 actatttgcg gaaagataga tcatgacgga tattatgtga aaatgggaaa tgcatgggcg
     4981 ctttcggtat gttacgtaaa atttccggaa aagacaaggg cttttcttga gaaggatacg
     5041 ttggatgatt ttacacataa taaagcaata caaaaaatca gagaatctta cagagtcagt
     5101 aaggaagaaa aagaacaact gacttgtttg aaacgctgaa aataaaatca aagaaaagac
     5161 agactggaga atcagatgga agcagataac aggcagtttg acggaaaact ttttctgaaa
     5221 gatgtgaata gaaagctaaa cgaccggttg gcagaaattc aaaaagatct taatcaggga
     5281 ttaaaagaaa ttgaaaatat gcatgaatat tactgggaaa attatacgga aatggatcag
     5341 tacggatatg aggactatga caatcagcag gcgctcttac agcaggtaaa tgcaaacaga
     5401 gataagtcaa agttgatgaa aagacttgaa aaaatgattg attctccgtt tttcggaagg
     5461 gtagatttcc tgtttgaagc agaagaagag gaagaaccgt tttatatcgg tatcggaaat
     5521 ttcggagagc gtgcgggaat gacgcctctt atttatgact ggagggctcc tgtgagcagt
     5581 ctgttttatg actttgataa aggtgaggca ttttacgaag cgccatcagg agtgatcaaa
     5641 ggagaggttg tttcaaaatg gcagtataaa atacgcggcg gcaagatgat atatgggttt
     5701 gaaagcgatg ttaagattga cgatgagatc ttaaagcatg agcttggaaa caacggagat
     5761 gtcaaactga aaaatattgt tcgaacgatt cagaaagaac aaaatgcaat catccgaaat
     5821 acgaaggata aaattcttgt catacaaggc gcagcgggaa gcggaaagac ttcgatcgcc
     5881 cttcaccgga tcgcttactt gctttatcac gacaggaaaa atttaaaatc atcgaatata
     5941 ttgattcttt caccaaacag tgtgtttgca gattatattt ctcatattct tccggaactt
     6001 ggcgaggaaa atataaaaga aatgagtttt gatctcttcg cttataaaga acttcaggac
     6061 acggcggcag actgtgagga tagatatgat cagttagaac gtatgatgaa gtttgatgat
     6121 cgaagagcgc aggaacgttt tgagtggaaa cagtcggcgg gatttgtagg cgaggcagaa
     6181 ggttttttcg cggtacttga agatgagctg atggaatttc gagaggtgga gttcaggggg
     6241 atgcgttttt cggaagaaga aatcattcat ttgttttacg ataagtttta tcagataccg
     6301 cttctgaaaa gaatggatgc ggttatggaa tattttattg attcttatga gacgttaaaa
     6361 ggcagaaata ttgaagagga agacagagaa tttcttcaga gaaagtttga caaaatgtat
     6421 gtaacgaaag atatttatgc tatttacaat aaactgcttg cggtatgcgg gcaggaacaa
     6481 atggcagaac tgccttatga acgaaggaaa atccaatacg aagatgtatt tccgatgctg
     6541 tatgtaaaat acagactgac aggaaagaat gagcataaaa atattaagca tcttgtaatt
     6601 gatgagatgc aggactactc ttatttgcag tatcttatct tgaaggaact gtttgactgt
     6661 aaaatgacaa tactcggaga tcgtgcccag actttgggaa aagaacaaca ggatgtttta
     6721 aaattccttc ctaaagtatt tgggaaaaat ataagaaccg ttattctgaa taagagttac
     6781 cgcaatacga gagaaatcgc agattatgcc ggtaagatcg cgaatgtgaa agatattgaa
     6841 tttttggaca gacatgggaa agatgtggca gaaagaaa
//
LOCUS       DBMDXF010000097         6814 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2864_length_6814_cov_1.856100, whole genome shotgun sequence.
ACCESSION   DBMDXF010000097 DBMDXF010000000
VERSION     DBMDXF010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2864_length_6814_cov_1.856100"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(141..752)
                     /locus_tag="ACZPD4_05095"
                     /pseudo
     CDS             complement(141..752)
                     /locus_tag="ACZPD4_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
     gene            747..944
                     /locus_tag="ACZPD4_05100"
                     /pseudo
     CDS             747..944
                     /locus_tag="ACZPD4_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320599.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
     gene            1183..2502
                     /gene="rsxC"
                     /locus_tag="ACZPD4_05105"
     CDS             1183..2502
                     /gene="rsxC"
                     /locus_tag="ACZPD4_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR1378484.1"
                     /translation="MGLLTFKGGIHPDDGKRFSKDSEIRTLLPKGELAYPLSQHIGAP
                     ANPIVKKGDHVLKGQKIAEAGGFVSSPIHASVSGTVKGIEYRFNPAGTKTECIIIEND
                     GEYAEINDLTVKPFGEMTREEIIERIGEAGIVGMGGAGFPTRVKLSPKEPEKIEYIIA
                     NCAECEPYITADYRRMLENTGQLVNGMRIILSLFPNAKGIFAVEDNKKDCIEKLNKET
                     ENDPNMEVKALMTKYPQGAERQLIYAVTKRAINSSMLPADAGCIVDNVETLIGIHMAV
                     AEGKPLTERVVTVSGDAINSPGNFKVLLGTNHMELIEAAGGFSQQPEKLISGGPMMGF
                     AMVSAEAPVTKTSSSILAFKEDIVSKSVETACINCARCVDVCPSRIIPSRLADLAQRH
                     DEKAFVAMNGLECVECGSCSYVCPAKRPLKQAIGSMRKIALANKRKK"
     gene            2521..3453
                     /locus_tag="ACZPD4_05110"
     CDS             2521..3453
                     /locus_tag="ACZPD4_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1378485.1"
                     /translation="MNENYHVSASPHIRDKIKSSNIMLLVVIALLPATVFGIWNFRHE
                     NAWILVVATTAAAVLAEYIYEKLMHKPVTIGDFSAVVTGLLLALNLPPTLPWWMGVLG
                     AVFAIVVVKQLFGGLGQNFMNPALAARCFLLICFTQKMTYFVYDGVTGATPLAQLKSG
                     NVVNTMDMLLGNVRGTIGETSVIAIMIGAMFLILTGVIDLRIPGSYIVSFVIFITLFG
                     GNGFDPWYITAHLCGGGLMLGAWFMATDYVTAPITSKGKILYGICMGILTGLFRLFGA
                     SAEGVSYAIIISNLLVPLIEKVTLPKPFGKGGEK"
     gene            3453..4061
                     /locus_tag="ACZPD4_05115"
     CDS             3453..4061
                     /locus_tag="ACZPD4_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HIR1378486.1"
                     /translation="MNKIIKNTLILTAITVVSGLLLGIVYDVTKEPIAQAQERTKQKA
                     FQTVLPEASEFTSSNFDEKEAAKILKENGYTDDITEVAEATDKKGKVIGYVINVTSHE
                     GYGGDIEISVGIASDGTVKGIEMLSIGETAGLGMKAKDAEFKDQFRDKKVQKFTYTKN
                     GEKGDDKIDAISGATITTNAVTNAVDSALIYYQTELGGGINE"
     gene            4054..4791
                     /gene="rsxE"
                     /locus_tag="ACZPD4_05120"
     CDS             4054..4791
                     /gene="rsxE"
                     /locus_tag="ACZPD4_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR1378487.1"
                     /translation="MNKCAERLYNGLVKENPTFVLMLGMCPTLAVTTSAINGIGMGLS
                     TTAVLVMSNMLISMLRKVIPASVRMPAFIVVVASFVTIVDFLMAGFVPGLYKSLGLYI
                     PLIVVNCIILGRAESYASKNPVLPSIFDGIGMGLGFTVGLTAIGIVRELLGAGEVFGI
                     RVMPQSYEPLTIFVLAPGAFFVLACLVALQNKVKANLEKKGKKVPVSSGCGEGCSACA
                     SKGICGGHLAENEPHHEETNVKEEEEA"
     gene            4792..5403
                     /locus_tag="ACZPD4_05125"
     CDS             4792..5403
                     /locus_tag="ACZPD4_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HIR1378488.1"
                     /translation="MQELLLIAVGAAFVNNVVLSQFLGICPFLGVSKNVKTAAGMGGA
                     VVFVITIASFVTGLIYKFILGNPKILGGELQYLNTIVFILVIAALVQFVEMFLKKVMP
                     PLYNALGVYLPLITTNCAVLGVALTNVQNGYYEKGVGIGLLTGVVNGFATAAGFFVAI
                     VLMAGIREKIEHNNVPEAFKGTPIVLVTAGLMAIAFCGFSGLI"
     gene            5417..6223
                     /locus_tag="ACZPD4_05130"
     CDS             5417..6223
                     /locus_tag="ACZPD4_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR1378489.1"
                     /translation="MNITAILLAAAIVGGAGLFIGIFLGVSGKKFAVEVDEREEAILD
                     VLPGNNCGGCGYAGCSGLAAAIAQGKADVGGCPVGGASVAGKIGEIMGVDASASERKT
                     AFVKCGGTCEKTKSEYEYYGIKDCIMASQMQDGGAKGCAYGCLGYGSCVDACPFDAIH
                     IVDGIAVVDKEACKACGKCVDACPKHLIELIPYKQKTFVQCNSNEKGKALMDVCQVGC
                     IGCRLCERNCEAGAITVNNFLAHIDADKCTNCGVCAEKCPRKIITVRFEA"
     gene            6285..6641
                     /locus_tag="ACZPD4_05135"
     CDS             6285..6641
                     /locus_tag="ACZPD4_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1378490.1"
                     /translation="MKKNDNILILGILFIALVVLGIQLLHSANGTEKVKITVDGKVFG
                     TYLLSEDRTVSVNETNIVLITEGTVSMESADCPDQICVHHQRIKRSGESIICLPNKVV
                     VSIEGGGEEKLDGTVQ"
     gene            6657..>6814
                     /locus_tag="ACZPD4_05140"
     CDS             6657..>6814
                     /locus_tag="ACZPD4_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1378491.1"
                     /translation="MRTRAAYFGIFTALALILGYVELLIPIHFGIPGAKLGLANLITI
                     LVLYKMGW"
BASE COUNT         1919 a         1244 c         1809 g         1842 t
ORIGIN      
        1 tcgaggtgtt cgcgggcgct ttcggtatag gggatgaaca gcgtcgcatc gagtgtttcg
       61 ggcagcgtgt tgggtgcctg cgcttcggtg aggaagtgcg ggcagtgcgc caccgggatc
      121 gcgcggccga attcgacctc aggcttcacg agcagcgccg aaagatcgac caagttggcc
      181 ttccagctgc gcgcatcgtg cgtctgacgc aggcattcca cctggcccac catctcgtcg
      241 atcgtggcga acccaagctc ggccatgatc tcgcgtgttt gctcggccac catcatcatg
      301 tagttcacca cgtattccgg cttgccgcag aagcacgccc gcaactcgcg gttttgcgtg
      361 gcgatgccca ccgggcaggt gtcttgctgg caatcgcgct gcatcaagca gcccatcgcg
      421 atgagcggca tggtggcgaa tccgaattct tcggcgccca agaggcaggc gatcgccaca
      481 tcgcggccat ccatcaactt gccatcggct tcgagcacga tgcggctgcg caggccgttt
      541 tgcagcaatg tctgttgcgt ttctgccagg cccatctcga gcggcaggcc tgcatgatgg
      601 atcgaatcgc gcggggctgc acctgtgcca ccgttcgaac cggagatgag gatcttgttc
      661 gcaccgccct tggcaacgcc ggttgcgatc gtgcctacgc ccgtttcgga aacgagcttg
      721 accgatacgc gcgcgccggg gttggcgttc ttcatgtatc tcagatttcg agagaacatg
      781 ttgagaaacc atcggatgtt ctttctgtag gtcaggagat tacagctaag attgttgacc
      841 tgaacgaaga agagaagaag atcagcctga gcatgaaagc attggaagct gatcgtgatg
      901 aagaagatgc agtatctgtt gattacgaag cagatgctga gtaattatct tttgtatttt
      961 atataatgga taagataggg gacgatactt gcatagagtg tcgttctctt tattatgtta
     1021 aaaagataac attcttctga aaatacagaa ttgtatgatt ttgtcgtttg aatgactgga
     1081 tttttatgct tcattctagt aaaataacgt aatatgaaaa taatgcggga atagagtgtg
     1141 ataagtagat tctgtttcgg cgggaaagaa aggaagaata tcatgggact tctgacattt
     1201 aaaggcggta tccatccgga tgacggaaag cgtttttcaa aagacagtga aatacgtaca
     1261 ttactgccga aaggggaact ggcgtatccg ctttcacagc atatcggagc gcctgcgaat
     1321 ccgatcgtaa aaaaaggaga tcatgtttta aaaggacaga agattgcgga agcaggaggt
     1381 tttgtgtcat caccgatcca tgcttctgta tccggaactg tgaaaggaat tgaatatcgg
     1441 tttaatccgg caggtacaaa aacagaatgt atcatcattg aaaatgatgg agaatacgcg
     1501 gaaataaacg atcttacggt gaaacctttt ggtgagatga caagagaaga gatcattgag
     1561 agaatcggtg aagccggtat tgtcggaatg ggaggcgcag gttttcctac gagggtaaag
     1621 ctttctccga aagaacctga aaagatagaa tatattattg cgaactgtgc agagtgtgaa
     1681 ccgtatatta cggcagatta cagaaggatg ttggagaata ccggacaact ggtgaacggt
     1741 atgcgtataa tcctttcgct gtttccgaat gcaaaaggaa tctttgcggt agaagataat
     1801 aaaaaagact gtattgaaaa attaaacaaa gaaacagaaa atgacccgaa tatggaagtg
     1861 aaggcactca tgacaaaata tcctcaggga gcggaacgtc agttgatcta tgcagtgaca
     1921 aaacgtgcga tcaattcatc tatgctcccg gcagatgcgg gttgtattgt tgataatgtg
     1981 gaaacattga tcgggattca tatggcagta gctgagggga aacctcttac ggaacgtgtt
     2041 gttactgtaa gcggagacgc cataaattct ccgggaaact ttaaagttct tcttggaaca
     2101 aaccatatgg aattaattga agcggcgggt ggattttctc aacagccgga aaaattgata
     2161 tccggagggc ctatgatggg attcgccatg gtaagtgcag aagctccggt aacaaaaaca
     2221 tcttcttcta ttcttgcgtt taaggaagat atagtttcaa aaagcgtgga gacggcatgt
     2281 atcaactgtg caagatgcgt tgatgtctgt ccgagtcgta ttataccttc acgattagcc
     2341 gatcttgccc aaagacatga tgaaaaggct tttgttgcga tgaatggact tgaatgtgtg
     2401 gaatgtggat catgcagtta tgtgtgtccg gcgaaaagac ctttgaaaca ggctatcggt
     2461 tctatgagaa aaattgcgct tgcaaataaa agaaagaagt aagtgagagg tgagagaaaa
     2521 gtgaatgaaa attatcatgt atcagcatcc ccgcatattc gggataagat aaaaagcagc
     2581 aatatcatgc ttcttgttgt gattgccctg ctccctgcta ctgtattcgg aatttggaat
     2641 tttagacatg aaaatgcgtg gatcttagtt gttgctacaa cggcggctgc tgtgcttgca
     2701 gagtatatct atgaaaaatt aatgcataaa ccggttacaa tcggtgattt cagcgcagtt
     2761 gttacaggtc ttttacttgc attgaatctt cctccgacgc ttccgtggtg gatgggggta
     2821 ctcggagccg tatttgcaat cgttgttgta aaacagcttt tcggtggatt gggacaaaat
     2881 tttatgaatc cggccttagc tgcaagatgc tttcttctaa tctgttttac gcaaaaaatg
     2941 acgtattttg tgtatgacgg cgtgacaggc gcaactccgc ttgcacaatt aaagagcggc
     3001 aatgtagtaa atacgatgga tatgcttctt ggaaatgtcc ggggaacgat cggagagaca
     3061 tcggtgatcg cgattatgat cggagcgatg ttcttaatat tgacaggagt gatagatctt
     3121 agaattccgg gatcttatat cgtatcattt gttattttta tcacgctttt cggcggcaat
     3181 ggatttgatc cgtggtacat aaccgctcat ttatgcggcg gcggtctgat gcttggagca
     3241 tggtttatgg caacagatta tgtgactgct ccgattacga gcaaaggaaa gattttgtat
     3301 ggcatttgta tgggaatctt aaccggactg ttccgtttgt tcggagcttc ggcagaaggg
     3361 gtatcttatg caatcatcat cagtaacctt ttggttccgt tgatcgagaa ggttactctt
     3421 ccgaaacctt tcggtaaagg aggagaaaag taatgaataa aattataaaa aatacgttga
     3481 tcttaactgc aataacagtt gtttcaggac ttttgctcgg aattgtttac gatgttacaa
     3541 aagagccgat tgcgcaggcg caggagcgta caaaacaaaa ggcgtttcag acagtgcttc
     3601 ccgaggcttc agagtttaca tccagcaatt tcgatgaaaa agaggcggca aaaattttaa
     3661 aagaaaacgg ttatacagac gatataacag aagtagcgga agcgacagat aaaaaaggga
     3721 aagtaatcgg ttacgtgatc aatgtcactt ctcacgaagg gtatggcggc gacattgaaa
     3781 tttctgtagg aatcgcctcg gacggaacag tgaaaggaat tgagatgctc tccatcggag
     3841 agacggcggg attaggaatg aaagcgaaag acgctgaatt taaagatcag tttcgggata
     3901 aaaaagtgca aaaattcact tatacgaaaa atggagaaaa aggcgatgat aagatcgatg
     3961 ccatcagcgg cgctacgatt acgacaaatg cagtgactaa tgccgttgat tcagcactga
     4021 tttattatca aacggagtta ggaggcggta tcaatgaata aatgtgcaga acgtctttat
     4081 aatggccttg tgaaagaaaa tcctacgttc gttctcatgc tcggtatgtg tccgactctt
     4141 gcggttacaa catctgcgat caacggaatc ggtatggggc tttctacgac agcggtactt
     4201 gtgatgtcaa atatgttgat ttctatgttg agaaaagtga ttccggcatc agtgcgtatg
     4261 cctgctttta tcgttgttgt agcttctttt gttacgatcg ttgatttttt gatggcggga
     4321 tttgttcccg ggctttataa atcactcgga ttgtatatcc ctttgatcgt agtaaactgt
     4381 attattttgg ggagagcgga aagctatgca tcaaaaaatc cggtgcttcc ttcgattttc
     4441 gacgggatcg gtatgggact tggattcacg gtcggtctta cggcaatcgg tattgtgaga
     4501 gaactgcttg gtgcaggcga ggtgttcggc attcgtgtta tgccgcagtc atatgaacct
     4561 cttacgattt ttgttcttgc cccgggtgcg ttttttgtat tggcatgtct tgtagctctt
     4621 cagaataaag tgaaagcgaa tcttgagaaa aaaggaaaaa aagttccggt atcgtcagga
     4681 tgcggcgaag gttgctctgc atgtgcaagc aaaggaatct gcggaggtca tctcgcagaa
     4741 aacgaaccac atcatgaaga gacgaatgta aaagaagagg aggaggcata aatgcaggaa
     4801 ttacttttga ttgcagtcgg agctgcgttt gtaaataatg ttgtccttag tcagttcctc
     4861 ggtatctgtc cgtttttagg tgtttctaaa aatgtaaaaa ctgcagccgg aatgggcgga
     4921 gcagttgtat tcgttatcac aatcgcttcg tttgtaacag gattgatcta taaattcatc
     4981 cttggaaacc cgaaaatttt aggaggagag ctccaatatt taaacactat cgtatttatc
     5041 cttgtcatag cagctcttgt acagttcgta gagatgtttt taaagaaagt gatgcctccg
     5101 ttatataatg ccctcggcgt ttatcttccg ctgattacaa cgaactgcgc ggttcttggc
     5161 gtagcgctga caaacgtaca aaacggatat tatgaaaaag gtgtcgggat cggtttgctg
     5221 accggtgtag tgaacggatt tgcgacagcg gcgggatttt ttgttgcgat tgttcttatg
     5281 gccggaattc gggaaaaaat cgaacacaat aatgttcccg aagcatttaa aggaactccg
     5341 atcgttcttg tgacagccgg actgatggca atcgcatttt gcggattttc aggattaata
     5401 taggaggtgc gatagaatga atataacagc gattcttttg gctgccgcga tcgtcggtgg
     5461 agccggatta tttatcggta ttttcctagg tgtatccgga aaaaaattcg cagttgaagt
     5521 tgatgaaaga gaagaggcga ttctcgatgt tttacctgga aataactgtg gaggctgcgg
     5581 ttatgcagga tgttcaggcc tcgctgcggc gattgctcag ggaaaagcgg atgtaggagg
     5641 atgcccggtc ggaggcgctt ctgtagctgg gaagatcggt gagattatgg gcgtagacgc
     5701 ctcggcaagc gaaagaaaaa ctgcatttgt aaaatgcggc ggaacatgtg aaaagacaaa
     5761 aagtgaatat gaatattatg gaatcaaaga ctgtatcatg gcaagtcaga tgcaggatgg
     5821 aggagcaaaa gggtgtgcat acgggtgtct cggatatggc tcatgcgtgg atgcctgtcc
     5881 gtttgatgca atccatattg tagatggaat agcagttgtt gataaggaag catgtaaagc
     5941 atgtggaaaa tgtgtggatg catgtccgaa gcacctgatc gaattgatcc cgtataagca
     6001 gaaaacattt gtgcagtgta actcaaatga gaaagggaaa gcattaatgg acgtctgcca
     6061 agtcggatgt atcggatgtc gtctctgcga gcgaaactgc gaagccgggg ctattacagt
     6121 taataacttt ttggctcata tagatgccga caaatgcaca aattgcggcg tatgtgcaga
     6181 aaaatgtcct cgaaagatca taacagtacg gtttgaagcg tgagtaaatg aagcgttaaa
     6241 agtgtagtgt atcgccggga ttttcaacgt gtgtgaggtt agaaatgaag aaaaacgata
     6301 acattcttat acttggcatc ctgttcattg ctctggtcgt gctgggaatt cagctattac
     6361 actcggcaaa cggtacggag aaagtaaaaa ttactgtaga cgggaaggtt tttggcacat
     6421 atctgttgtc tgaagatcga actgtttcag tgaacgaaac gaatatagtt ttaattacgg
     6481 agggtacggt ttcaatggag tccgctgatt gtccggatca gatttgtgtt caccatcaga
     6541 ggatcaaaag aagtggagaa agtattatat gtcttccgaa taaagtcgtt gtgtcgatag
     6601 aaggcggagg cgaagaaaaa cttgacggaa ctgtacaata gtcaggagag agtgatttga
     6661 gaaccagagc tgcatatttc ggtatattta cagcgctggc attgatttta ggttatgtgg
     6721 aactgctgat tcccattcac tttgggattc ccggagcaaa actgggactt gcgaatctta
     6781 taacaattct tgttctatat aaaatggggt ggaa
//
LOCUS       DBMDXF010000098         6812 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2868_length_6812_cov_1.966026, whole genome shotgun sequence.
ACCESSION   DBMDXF010000098 DBMDXF010000000
VERSION     DBMDXF010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_6812_cov_1.966026"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="ACZPD4_05145"
     CDS             complement(<1..329)
                     /locus_tag="ACZPD4_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR1378492.1"
                     /translation="MSEKAESGTVLAVCVSREKGTQKQNVGSAHFIEDFGIEGDAHAG
                     KWHRQVSLLSHDKIEAFRAKGAEVIDGAFGENLVVTNLDFRSFPVGTRLICRDVILEM
                     TQIGKEC"
     gene            complement(410..1381)
                     /gene="moaA"
                     /locus_tag="ACZPD4_05150"
     CDS             complement(410..1381)
                     /gene="moaA"
                     /locus_tag="ACZPD4_05150"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR1378493.1"
                     /translation="MNDQYGRRIDYMRVSVTDRCNLRCVYCMPGEGFSFLPHEDILSF
                     EEIERVCRIGVRLGITKIKLTGGEPLVRRGIPDLVGKLKGIPGIDEVTLTTNGILLKE
                     QINELVSNGLASVNISIDTLDERRYFRMTRGGDIKKALEGMESALKYPDLKVKVNCVP
                     FCEEDCISLALLAKKRKIDVRFIEMMPIGNGKNFNGKMSEEIYKLLHNEFGDYEIYKK
                     RVGNGPAEYIRFHNFDGRIGFISALSHKFCYKCNRIRLTSEGFLKPCLQYGNGTDVRE
                     MLRNGADDSLIEEKMREAVFFKPDGHEFGRSGKGEEIETKKMSQIGG"
     gene            complement(1378..2454)
                     /gene="moaCB"
                     /locus_tag="ACZPD4_05155"
     CDS             complement(1378..2454)
                     /gene="moaCB"
                     /locus_tag="ACZPD4_05155"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HIR1378494.1"
                     /translation="MGLTHFDENGKAVMVDVTDKKETAREAVAEGKILVNRDVFQAVK
                     AGTVGKGDVLAVAATAGIAGAKRTSDLIPMCHILPLTNCKVDFRMNEKECAIFCTCTV
                     KVTGKTGVEMEALTGVSAALLTIYDMCKALDKKMEITDIRLLKKTGGKSGDIWNDFDR
                     CAGLVGENLSAENRSAGNAEAEDVENVCRKKEKRTRRWKAAVVTMSDKGFAGEREDKS
                     GPLICEMIADQGYDVVRTELLPDEQKEIEKCLCTLCDDEKVDIVFTTGGTGFSLRDCT
                     PEATMAVAHRNVPGIAEAMRAASAEVTPRAMLSRAVSAIRNHTLIVNLPGSPKAVKEN
                     LEAVLPVVEHGLEILTGQAGECGR"
     gene            complement(2611..4548)
                     /locus_tag="ACZPD4_05160"
     CDS             complement(2611..4548)
                     /locus_tag="ACZPD4_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1378495.1"
                     /translation="MNEQKMNYEHVLSPIKIGPMTLKNRVSFTPVWPSFATADGHVNR
                     ELIEWVRDIVKGGAACINIGCGCVNRNLPPFVTHLLRMSEESVVNEMTILSDLVHMYQ
                     CKIGIELFAINMYGGNFADRDHNGDKPAVELDPTYMTKEQIKEYIEDFALAAERAVRC
                     GVDSLVIHGAHGQLPGCFLNKRINERTDEYGADTIENASRFTVELLAAVREKIKDRLA
                     IEYRINANDMIEGSPSLDEVIAFVKRIEPYIDLLHVSRGMHSEQKLAPYMNQPIYYDH
                     GINIEDAAKIRKEISVPVTVVGSVTLEQAEQYIAEGKADMVSMARGLMADPMVVKNAK
                     SGCPENTRPCVRCNYCINRTHYDLAPVRCSVNAELGMETLYMNLGNTLPKRIAVIGGG
                     PAGIEAARTAAQRGHTVDLYEKEDHLGGVLTMAGAPKFKQDIKKYVEWTIHSISGQER
                     VSVHLNSEVRAEDLKGKDYDAVIVAVGAEPVIPKFAKGREDVVWVGDVELGKVSAKKQ
                     VVIVGAGLTGCEAALSLAKAGKEVTLVDMIDVCDFGRGGAKFNQIALRGLLEAEQVKM
                     IGGVKMNIEGDRIVFVKNEKTIELSYEQIILSLGVKANEEKVQAFADAGREVVFVGDC
                     NTKIGTLYHAVHTAHEAASVL"
     gene            complement(4574..5518)
                     /locus_tag="ACZPD4_05165"
     CDS             complement(4574..5518)
                     /locus_tag="ACZPD4_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HIR1378496.1"
                     /translation="MGKLLLTGVDGNLGETAAEILLTLEPKENLIFCGYSEEALKKYA
                     DMGVETRQTNFNNADGLSEAFKGADALALISMPFVGAKRQKAHKNAIDAAKAAGVKQI
                     IYTSLVNADDETNPSVEKKDHIYTENYIKEVGLDYQFMRNSQYAEAMVTNYFTYVKMG
                     TPLTNSQGDGLMAYISRKDCAKAVAYALHRSGEFTRKVWDINGPELMTITQFIEIGNE
                     STGNNLTFQNVTDEENYQIWDAMGVPRTTDGVFLEDSEAPFSSDGMVTFAQAIREGKM
                     DIFTDDFKKLTGDDPITVKYMFDHNEDFQIGARHSKDD"
     gene            complement(5601..6473)
                     /locus_tag="ACZPD4_05170"
     CDS             complement(5601..6473)
                     /locus_tag="ACZPD4_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1378497.1"
                     /translation="MSVVETGSFSQAAKKHYLSQAAISQQISKLEEEVGVRLFDRSKY
                     RPELTEAGKYYYQKIKQLSEEYEKIEKQTRYIQKKNSQKIVVGITGPFENKYLPRIIN
                     EYKEGHETHFDVRVYTFEEGKKLLEAGDIDIAFGLSSEFSVNPNISYETIHQSETCIV
                     CSRKNRLSQYKSINPILVKDEPMIALSSQMGRKFYDDFMKAFMLDGFEPNIVKEVNSL
                     SEYFISIKLDEGIGYTGREVVPEEDDLCTVKIVGSHHHADFAVAYLKENKKQSVVQFY
                     EYIVAYFKDYKKNL"
BASE COUNT         1711 a         1847 c         1076 g         2178 t
ORIGIN      
        1 tgacactctt ttccgatctg cgtcatctct aagatcacat cgcggcatat aagccgtgtt
       61 ccgaccggaa aagagcgaaa atccaagttt gttactacaa gattctctcc gaaagcgccg
      121 tcgatcactt ctgctccttt tgcccgaaac gcttcgatct tatcatgaga aagaagactc
      181 acctgccgat gccattttcc ggcatgagcg tcgccttcta tcccaaaatc ttctataaaa
      241 tgtgcgctgc ccacattttg cttctgcgtt cctttttctc tgcttacaca gactgcgagc
      301 actgttccgc tttctgcttt ttctgacatt tttctcacac tccccgctat ctttgtttta
      361 tcttctttat cttattgctc tgattttttg ttctgatttc tttttcctgt caaccgccga
      421 tctgagacat cttttttgtt tcgatttctt cgccttttcc agatcttccg aactcatgac
      481 cgtcaggttt aaaaaacacc gcttctctca tcttctcctc gatcaaactg tcatccgccc
      541 cgtttcttaa catttcccga acgtctgtgc cgtttccgta ttgcagacac ggctttaaaa
      601 atccttccga agtgagtctg atccgattac atttataaca aaatttatgc gaaagggcgc
      661 tgataaatcc gattctccca tcaaaattat gaaaacgaat atactctgcc ggtccgtttc
      721 ctactctttt tttatatatt tcataatcgc cgaattcgtt gtgaagcaat ttatatattt
      781 cttcagacat ttttccgttg aaatttttcc catttccaat aggcatcatc tcaatgaatc
      841 tgacatctat tttccttttt ttcgccaaaa gcgcaagaga aatgcaatct tcttcacaga
      901 aaggcacaca atttactttc actttcagat caggatattt taacgcagat tccatgcctt
      961 ccaacgcctt ttttatatca ccgccccgcg tcatccgaaa ataacgtctt tcgtcaagcg
     1021 tatcaatgct tatattaaca gaagcaagcc cgtttgaaac taattcgttt atttgttctt
     1081 ttaataatat cccgtttgtt gtcaacgtca cttcatctat acccggaata ccctttaact
     1141 tcccgacaag atccggtatt ccccgccgca caagcggctc tccgcctgtc agcttgattt
     1201 tcgtaatgcc aagtcttacc ccaatcctgc acactctttc aatctcttca aaagaaagga
     1261 tgtcttcatg cggaagaaag gaaaagccct ctcccggcat acaataaaca caccgcagat
     1321 tacacctgtc tgttacggat acccgcatgt aatcaatccg cctgccgtac tgatcgttca
     1381 tcgtccgcac tctcccgcct gtcctgtcaa tatttcaagt ccatgttcca ctaccggaag
     1441 aactgcttcc agattttctt ttaccgcttt cggactgccc ggaagattca cgatcaacgt
     1501 atgatttcgg atcgcggaaa cagctctgct caacattgcc ctcggcgtca cttccgccga
     1561 agctgctctc atcgcctctg cgatccccgg aacattccga tgtgcaactg ccattgtagc
     1621 ctcaggagta caatcacgca aagaaaaccc tgtacctcct gtcgtaaata cgatatcaac
     1681 cttttcatca tcacaaagtg tacatagaca cttttcaatt tctttttgct catccgggag
     1741 aagttccgtt ctcacaacat cgtatccctg atccgcgatc atctcacaga tcaacgggcc
     1801 gctcttatct tctcgttctc ccgcaaatcc tttatcactc atcgtaacga ccgcagcttt
     1861 ccatctgcgc gtccgtttct cttttttgcg gcacacgttc tccacatctt ctgcctctgc
     1921 atttcccgca gatctgtttt ctgccgacag attttcaccg acaagtccgg cacaccggtc
     1981 aaaatcgttc catatatctc cgcttttgcc tccggtcttt ttcaaaagac ggatatcggt
     2041 aatctccatc ttcttatcca atgctttaca catatcgtaa atcgtaagaa gcgcggcgct
     2101 cactcctgtc aaagcctcca tctcaactcc ggtctttcct gtcactttca ctgtacaggt
     2161 acagaagatc gcacattctt tctcattcat acgaaaatcc accttacaat tcgtaagcgg
     2221 caatatatga cacataggaa tcagatctga ggtccgcttc gcccctgcaa tacccgccgt
     2281 tgccgcaaca gccaaaacgt cgcctttacc gactgtccct gctttcacag cctgaaaaac
     2341 atcccggttt acaagaatct tcccttctgc cacagcctct cgtgccgtct cttttttgtc
     2401 ggtcacatcg accatgaccg cttttccgtt ttcgtcaaaa tgtgttaatc ccatatagag
     2461 tcctttccgt tctcttacat ttttccaaat cattttttat tctaccacag tttcataaat
     2521 ttttcgacga caagattgta aaaaaagaat gccctgtacc gtgttgcagt acaaagcact
     2581 ctttttgaat cttctttcgt atatttacat ttataaaaca gacgccgctt catgagcggt
     2641 atgaaccgca tgatataatg ttcctatttt cgtattacag tctccgacga aaacaacttc
     2701 tctccccgca tccgcaaatg cctgtacttt ctcttcattt gcttttaccc ccagcgaaag
     2761 gatgatctgt tcatatgaaa gttctatcgt tttttcattt tttacaaata cgatgcggtc
     2821 gccctcaatg ttcattttga caccgccgat catcttgacc tgttctgctt ccaacagtcc
     2881 gcgcagggca atttgattaa atttggcgcc tcctcttcca aaatcacaga catcgatcat
     2941 gtcaaccaaa gtaacttctt ttcccgcttt tgccaaagac agcgctgctt cgcatccggt
     3001 aagtcctgcg cctacgatca caacttgctt ctttgcagaa actttcccaa gctccacatc
     3061 tccgacccat acgacatcct ctctgccttt cgcaaacttc ggaataacag gctctgctcc
     3121 gactgccacg atcaccgcgt catagtcttt tcccttcaga tcttccgccc gaacttcact
     3181 gttcaaatga acagaaaccc tctcttgacc ggaaatagaa tgaatcgtcc attctacata
     3241 ttttttgata tcttgcttga atttcggagc gcctgccatt gtgaggactc cgcctaaatg
     3301 atcttctttt tcataaagat ctactgtatg ccctctttgc gccgccgttc tcgccgcctc
     3361 gattcccgca ggtcctccgc ctatcacggc aatcctctta ggcaaagtgt tccccagatt
     3421 catatacaat gtttccatcc caagttctgc gtttacagaa cacctcaccg gagcaagatc
     3481 ataatgcgtc cggttaatac aataattgca tctgacgcat ggtcttgtat tctcagggca
     3541 tccgcttttg gcgttcttaa ccaccatcgg atctgccatc agtcctcgcg ccatagaaac
     3601 catatcggcc tttccttccg caatatactg ttccgcctgc tctaatgtaa ccgacccgac
     3661 taccgtcacc ggaacagaaa tctctttcct gatttttgcc gcgtcttcga tgttgattcc
     3721 atgatcgtaa tagatcggct gattcatata aggagcaagt ttttgctcgg aatgcatccc
     3781 tctggataca tgaagaagat cgatatacgg ctcaatccgt tttacaaatg cgataacttc
     3841 atccagggaa ggcgaaccct cgatcatatc atttgcattg atccgatatt cgatagccag
     3901 acgatctttg atcttctcac gaaccgcagc gagcagttcc accgtaaatc ttgatgcatt
     3961 ttcaatcgta tccgcgccat attcatctgt tctttcattg atcctcttgt tcaaaaagca
     4021 tcccggaagc tgcccgtgag ctccatggat cacaagactg tcaactccgc atcttaccgc
     4081 gcgctctgcc gccaacgcaa aatcttcaat atattccttg atctgttctt tcgtcatata
     4141 agtagggtca agctctaccg ccggcttatc gccgttgtga tctctgtctg cgaagtttcc
     4201 gccatacata ttaatggcaa aaagttctat tccgatcttg cactgataca tatggacaag
     4261 atctgataag atcgtcattt catttacgac actttcttcg ctcattcgca gcagatgagt
     4321 gacaaacgga gggagatttc gattgacgca gccacatccg atattaatac atgccgctcc
     4381 gccttttacg atatccctta cccattcaat caattcacgg tttacatgac cgtccgccgt
     4441 cgcaaaggac ggccatacag gagtgaaaga aactctgttt ttcaatgtca tggggccgat
     4501 tttgatagga cttaaaacat gttcgtagtt cattttctgt tcattcattt tccgatgttc
     4561 ctttcaaaag tatttaatca tcttttgaat gtcttgcacc gatctgaaaa tcttcgttat
     4621 gatcgaacat atatttcacg gtgattggat catcccctgt cagttttttg aaatcatcag
     4681 taaagatatc catttttcct tctctgatcg cctgcgcaaa cgtcaccatt ccatcgcttg
     4741 aaaacggagc ttcactgtct tccaggaata cgccgtccgt cgttctcggc actcccatag
     4801 catcccatat ttgatagttt tcttcatccg ttacattctg aaacgtcaga ttatttcctg
     4861 tagattcatt tccgatctcg ataaactgag taattgtcat cagctccggt ccgttaatgt
     4921 cccaaacttt ccgtgtaaac tcgcccgatc tgtggagcgc gtacgctaca gccttcgcac
     4981 agtcttttct ggagatataa gccatcaatc cgtcgccctg tgaatttgtg agcggagttc
     5041 ccatttttac atatgtaaag taatttgtga ccatcgcctc tgcatactgt gaatttctca
     5101 taaactgata atctaagccg acctctttta tataattttc tgtataaatg tggtctttct
     5161 tttcaacact aggatttgtc tcatcgtctg cattcactaa cgaagtataa ataatctgtt
     5221 ttactcctgc cgcttttgcc gcatctatcg catttttatg agctttctgc cttttcgctc
     5281 ctacaaacgg catcgaaata agagccagcg catctgctcc tttgaacgcc tccgacaatc
     5341 catctgcgtt attaaaattt gtctgacgag tttcaactcc catatctgca tattttttga
     5401 gcgcctcttc actataaccg caaaagatca gattctcttt cggttctaaa gtaagcaaaa
     5461 tttcagcggc agtttcacct aaatttccgt caacacctgt caataataat ttacccatcg
     5521 tttcatttct cctttgcttt tcatactgta atgataatat tttaacaagc aaaaagaaac
     5581 tacctgcttt tctataaaga ctataagttt ttcttataat ctttaaaata tgctacaata
     5641 tattcataaa actgaacaac actttgtttt ttattttctt ttaaataagc caccgcaaaa
     5701 tctgcatgat gatgactccc tactattttt acagtgcaca gatcgtcctc ttccggcaca
     5761 acctctctgc ctgtatatcc tattccttca tccagtttta tagatataaa atactccgaa
     5821 agagaattca cttcttttac aatattcggt tcaaacccat ccagcataaa cgctttcata
     5881 aaatcatcat aaaactttct gcccatctgc gaagataaag cgatcatcgg ctcatctttt
     5941 actaaaatgg gattgatact tttatactga cttaatctgt tttttctgct gcatacaata
     6001 cacgtttccg actgatgaat cgtttcataa gaaatgtttg gatttaccga aaactccgac
     6061 gacagaccaa aagcaatatc aatatctcct gcctctaaca gctttttccc ctcttcaaac
     6121 gtataaacac gaacatcaaa atgcgtttca tgcccttctt tatattcatt gataatacgg
     6181 ggaagatatt tattttcaaa cggacctgta atccccacta ctattttctg cgaattcttt
     6241 ttttgaatat aacgcgtctg tttctcgatt ttctcatatt cctcagataa ctgtttgatc
     6301 ttctgataat aatatttacc ggcttccgtc aattcagggc ggtatttact cctgtcaaat
     6361 aaacgaactc cgacttcttc ttccagttta gaaatctgct ggctaatggc agcctgcgac
     6421 aaatagtgct ttttcgccgc ctgcgaaaag cttcctgttt ctacaactga cataaaaaaa
     6481 taaaaactct tttttaacat gttgccacct cacctgttcc tgagaatact cgttctttaa
     6541 actgctttcc tttttagtat tctttcgtgt tatatgtgcg aaagatcttg cactcaagct
     6601 tcggttgtaa aaactcagtg cagttcaact cccatcatac cacatacaaa aatcatttgc
     6661 atccgatcag aactcaactc caccgtccga tcagacccca gttcgatttc atcgcttcac
     6721 tcttcatctc actgatgggc gtccgcccta tgcttaaaaa agaggactcc gcaccttgta
     6781 agcaagcaag cttgctcagc agagaacttc tt
//
LOCUS       DBMDXF010000099         6759 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2891_length_6759_cov_2.179483, whole genome shotgun sequence.
ACCESSION   DBMDXF010000099 DBMDXF010000000
VERSION     DBMDXF010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_6759_cov_2.179483"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="ACZPD4_05175"
     CDS             complement(<1..886)
                     /locus_tag="ACZPD4_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1378498.1"
                     /translation="MALKNSQYYALMREYEQTRLKNHDLQMKRYEEVYKKLPEFKSLD
                     ESISILSVQYGKKMLNGDPKALSSLKEELAILRQNKKNLLTSAGFPKDYLEPIYDCPD
                     CMDTGYIGNEKCHCFKRSVIELLYEQSNIKEFPTEASFDYFDLDFYPASQYDKTTGKN
                     ARAMMEETLNICHRFINNFGKEFNNLFFYGSVGVGKTFLSTCIAREIMEREFSVLYFS
                     APQLFSVLTQTKFDRNDVDSKNMNDYIFNCDLLIIDDLGSEYTNAFIAAQFFTCINER
                     LIHKKSTIISTNLSLESLA"
     gene            complement(934..2124)
                     /locus_tag="ACZPD4_05180"
     CDS             complement(934..2124)
                     /locus_tag="ACZPD4_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR1378499.1"
                     /translation="MKTLTLKNKFQTNATLLPNDFIDNYMVNANGEFIKVYLFLLRHA
                     EDDNPALTLSMIADCLNNTEKDILRALRYWESEGLLRSEYAADGKITSLELQSFACAW
                     TPPASRNTDSPAGGIAPQTVRSTIQNVSDRNLPSQPQSDISLSAGLTDPVHYIHNKEG
                     LHTASRQTASASNASIQKAVAIDEFRAQKEIKSLLFIAEQYLGKTLTHTEMETITYFY
                     DTLHMSADLIEYLIESCVENGHKSIHYIKKVAFSWVEEGIETVSQAKEQSMLYSRSCY
                     TVLNAFGIKGRGPAASELEFIKKWSEQYAFSSDIIAEACRRTITSTHQPSFEYADSIL
                     TKWHEHNIRHLKEISALDEAHQKQNSAVRTAARQKPVTKNLNNFERRSYDMESLEEQL
                     LKSN"
     gene            complement(2690..4075)
                     /gene="murC"
                     /locus_tag="ACZPD4_05185"
     CDS             complement(2690..4075)
                     /gene="murC"
                     /locus_tag="ACZPD4_05185"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1378500.1"
                     /translation="MYKIDFNEPIHIHFIGIGGISMSGLAEILLKEGFTVSGSDTKES
                     PLTKKLVSEGAHIIYGQKAENITRDIGCVVYTAAINRNNPELIEAVALKIPMLTRAEL
                     LGQLMKNYDTPIAVSGTHGKTTTTSMISHILLEGDLDPTISVGGILKAIGGNIRVGNS
                     GTFITEACEYTNSFLHFFPKISVILNIEEDHLDFFKDLEDIRHSFHQFAELLPEDGTL
                     IINAEIKDYAEIYEGLSCNVVTYGTNGCFDYSAKNITYDENGHVAFDLFRRGEYADHI
                     QLSVTGDHNVSNALSAIAVADILGLSIDITKRGLLSFTGTDRRFEYKGEFNGVTVIDD
                     YAHHPTEILATLKAAQHSLHNSVWCVFQPHTYTRTKAFFHEFAEALSHADHLVLADIF
                     AARETDTLGISSADLAKETEKLGTDTHYFPSFAQIEAFLKENCRPGDLLITMGAGDVV
                     TIGENLLKQTS"
     gene            4396..5670
                     /locus_tag="ACZPD4_05190"
     CDS             4396..5670
                     /locus_tag="ACZPD4_05190"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1378501.1"
                     /translation="MFKKEMIAMLLAGGQGSRLGVLTAKVAKPAVAFGGKYRIIDFPL
                     SNCINSGIDTVGVLTQYQPLRLNTHIGIGIPWDLDRNVGGVSILPPYEKSSNSEWYTG
                     TANAIYQNLDYMETYNPDYVLILSGDHIYKMDYEVMLDFHKANHADVTIAAMPVPIEE
                     ASRFGIVVTDHESRIKEFEEKPEKPSSNLASMGIYIFSWPVLKEALIKLKDQPNCDFG
                     KHIIPYCHDKGDRLFAYEYNGYWKDVGTLSSYWEANMELIDIIPEFNLYEEFWKIYTN
                     SANIPPQYIAQDGVVDRCIISNGTEVYGELHNSVLGGRVRVGKGSVVRDSIIMQGVTI
                     GENCVIDRAIIAEDTVIGDNVVIGIGSDVPNKMKPNIYSGGLATIGENSVIPSNVQIG
                     KNTAISGVTAAEDYEDGVLASGETLIKAGERV"
     gene            5667..>6759
                     /gene="glgD"
                     /locus_tag="ACZPD4_05195"
     CDS             5667..>6759
                     /gene="glgD"
                     /locus_tag="ACZPD4_05195"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIR1378502.1"
                     /translation="MRAVGLILAGGNSRKMRELTAKRAVAAMPVAGSYRAIDFVLSNM
                     TNSHIQKVAVLTQYNARSLNEHLNSSKWWDFGRKQGGLFVFTPTITADNGYWYRGTAD
                     AIYQNLDFLKKCHEPYVIITSGDAVYKLDYNKVLEYHIAKKADITVVCKDLEPGEDAT
                     RFGTVRMNEESRIEEFEEKPMVAHSHTISTGIYVLRRRQLIDLIEHCAEDERHDFVND
                     ILIRYKNLKKIYGYKINSYWNNISTVDAYYKTNMDFLKPEVRNYFFRELPSVYSKVSD
                     QPPAKYNPGAVVKNSLIGSGSIVNGTIENSVIFKKVFVGNNCVIKNSIILNDVYLGDN
                     TYIENCIVESRDTIRANTRYVGENGVEVVV"
BASE COUNT         1934 a         1282 c         1597 g         1946 t
ORIGIN      
        1 tggcaagcga ttcaagagaa agatttgttg agatgatcgt tgatttcttg tgaatgagac
       61 gttcgttgat acatgtaaaa aactgtgcgg caatgaacgc atttgtgtac tcgcttccaa
      121 gatcatcaat gatcagaaga tcacagttga atatatagtc attcatattt tttgaatcga
      181 cgtcattgcg gtcaaatttt gtctgcgtaa gtacactgaa aagctgcggc gccgagaaat
      241 agagaaccga aaattcacgt tccatgattt cgcgggcgat acaggtggaa aggaaagtct
      301 ttccgactcc gacgctccca tagaaaaaga gattgttgaa ttcctttccg aagttattta
      361 taaagcgatg gcatatattt aatgtctctt ccatcatagc gcgggcgttt tttcccgttg
      421 ttttatcata ctgggaagcc ggataaaaat caaggtcaaa atagtcgaaa gatgcttctg
      481 tcggaaattc tttgatattg gactgttcat ataaaagctc aataacggag cgtttgaaac
      541 agtgacattt ttcgtttccg atatatccgg tgtccatgca gtcgggacaa tcgtagatcg
      601 gttccagata atctttggga aatccggcag aagtaagaag gttctttttg ttctgccgca
      661 ggattgcaag ctcttctttg agagaagaca atgctttcgg atcgccgttt agcatttttt
      721 tgccatactg cactgaaagg atggaaatcg attcatccag tgatttgaat tccggaagtt
      781 ttttgtaaac ttcttcataa cgcttcattt gaagatcgtg atttttcaat ctggtttgtt
      841 cgtactcccg catcagtgca taatattgtg aatttttaag tgccatggag atactccttc
      901 tacttgccgt ctggctcaat gataatcgtt ttgttaattg cttttcagca gttgttcttc
      961 aagtgattcc atatcgtaac tgcggcgctc aaaattgttc agattctttg ttacaggttt
     1021 ttgccgcgcg gcagttctta cggcagagtt ctgtttctga tgagcttcgt caagcgccga
     1081 gatctctttt aagtgacgga tattgtgttc atgccacttt gtcagaatag aatcggcata
     1141 ttcaaagctc ggctggtgtg tagaggtaat cgtgcgtctg cacgcctctg cgataatgtc
     1201 agaggaaaat gcatactgct cagaccattt tttgataaat tcaagttctg aggcagcagg
     1261 tcctctgcct ttgataccga acgcattcag tacagtgtag cagcttctgg aatagagcat
     1321 agactgctct tttgcctgag agactgtctc gattccttct tcgacccatg aaaaagcgac
     1381 ctttttgata tagtggatgc ttttatgtcc gttttctaca caagattcga tcaaatactc
     1441 aatcagatct gccgacatat gcagtgtatc ataaaaataa gtaattgtct ccatttcagt
     1501 atgtgttaat gtttttccca aatattgttc tgcaataaag aggagagatt ttatttcttt
     1561 ttgtgcccga aattcgtcaa tagcgacggc tttttgaatg gatgcatttg aagcggatgc
     1621 tgtctggcgt gaagctgtgt gcagcccttc tttattatgt atatagtgaa ccggatctgt
     1681 cagacctgca gataaagaaa tatctgactg aggctgagaa ggaagattcc ggtcggacac
     1741 attttgtatc gtggagcgga cagtctgcgg cgcaatgcct ccggcaggag aatcggtatt
     1801 ccgggacgcg ggaggagtcc atgcacatgc aaaactttgc agttcgagag atgtgatttt
     1861 tccgtccgcc gcatattctg atcggagcag tccttcggat tcccaatatc taagcgcccg
     1921 caagatgtct ttttccgtat tgttgagaca atctgcaatc atagaaagtg tcagcgcagg
     1981 attgtcgtct tcggcatgcc gcagaagaaa aagatatact tttataaatt cgccgttggc
     2041 attgaccatg taattgtcga taaaatcgtt tggcagcagc gtcgcgtttg tctgaaattt
     2101 attttttaat gtcagtgttt tcatacttac tccatcctgt ttcattattc ttaagtgttt
     2161 tgtgaaggtc aaagcggcgc tgccgcgttt ttcccggttc agcccgcgcc aatcgcaaac
     2221 gcgtcctgcg gactcaaagc ggcgctgccg cggtttgctc atgaaaaagc gctcgccgta
     2281 tagggagtct cccagttcag cccgcgccaa tcgcaaacgc gtcctgcaga ctcaaagcgg
     2341 cgctgccgcg gtttgctcat gaaaaggcgc tcgccgtata gggagtctcc cagttcagcc
     2401 cgcgccaatc gcaaacacgt cctgcggact caaagcggcg ctgccgcggt ttgctcatga
     2461 aaaggcgctc actccatctc ggtacttgtt cgcaaagtca tacgagtatg acttatgctt
     2521 acaagcacgg agatgggctg aactggggtt aacaaaaagc ggacatggaa tccgcttttg
     2581 tcaggtgttt cttatatagt atcattttac tgccgggtaa aaaagggctt ttttgttggt
     2641 aacttgctct tttttgtaga taactatgtg gagaacttaa aaatagcttc tacgatgttt
     2701 gttttagcag attttcaccg attgtaacaa cgtctccggc tcccattgtg atgagcagat
     2761 caccgggacg gcagttttct ttcaggaatg cttcaatctg tgcaaagctt gggaagtagt
     2821 gcgtatctgt tccgagtttt tcggtttctt tcgccagatc tgccgaggag atgccgagag
     2881 tatctgtttc tcttgcagca aagatatcgg caagtacgag gtggtctgca tgagagagcg
     2941 cctctgcgaa ttcatggaag aacgcttttg tccttgtata agtatgaggt tggaatacac
     3001 accacacact gttgtgcaaa gagtgctgcg ctgcttttaa tgtggcaaga atctctgtcg
     3061 gatggtgtgc ataatcatca ataacagtta ctccgttaaa ttccccttta tattcaaaac
     3121 ggcggtctgt tcctgtaaaa gacaacaatc ctctcttagt gatatctata gaaagaccaa
     3181 gaatatcagc aacggcaatg gcggacagag cgtttgaaac attatggtcg cctgttaccg
     3241 acaactggat atgatccgca tattcaccgc ggcggaacaa gtcaaatgcg acatgtccgt
     3301 tttcgtcata agtaatattt ttcgcgctgt aatcaaaaca tccgtttgtt ccgtatgtca
     3361 cgacattgca ggaaagacct tcgtatatct cggcataatc tttgatctcg gcattgataa
     3421 taagagttcc gtcttcagga agcagttccg caaactgatg aaatgaatga cgaatatctt
     3481 ccagatcttt gaagaaatcg aggtgatcct cttcgatatt aaggattacg ctgatttttg
     3541 ggaagaaatg aagaaaactg ttcgtatatt cacatgcttc cgtaataaac gtacctgaat
     3601 ttccgacacg gatatttcct ccgatcgcct tcaaaatacc tccgactgaa atcgtcgggt
     3661 cgagatctcc ttccagtaaa atatgagaga tcatggacgt tgtcgttgtt ttcccgtgag
     3721 ttccggaaac tgcgatcggc gtatcgtagt ttttcataag ctgtccgagc agttctgcgc
     3781 gggtcagcat cggaattttc agcgcaactg cttcgatcag ttcaggatta tttcgattga
     3841 ttgccgcagt gtaaacgaca caaccgatat cccgtgtgat attttccgct ttctgaccat
     3901 agataatatg agcgccttca gaaaccagtt tcttcgttaa aggagattcc tttgtgtcgg
     3961 agccggatac agtgaatcct tcttttaaaa ggatttccgc aagtccgctc atgctgatac
     4021 cgccgattcc tataaaatgg atatggatcg gttcgttaaa atcaatttta tacataatgc
     4081 ttcctctttt ctttcaccta aatgggttga aagtaacagt ttcgtctgcg ccattcgtaa
     4141 aatcgcgctg tatagctgaa ctgttacaat taaaatgcac cgttatttct atgtgtaaca
     4201 atcgtttttt aaaaattagt cataaaatat cataacctac tatattatac tatatatgca
     4261 aaaaattgaa agaagttctt tttgtgcgaa ataaacaaaa agttaaaaat gttaaagaaa
     4321 aaattgtaaa aaatgattgg ataaacatga aaatatgata taataaatat atctaactag
     4381 aaatgaggtg acgtaatgtt taaaaaagag atgattgcaa tgcttttagc aggcgggcag
     4441 ggcagtcgat tgggggttct tacagcaaaa gttgcaaaac ctgccgtagc atttggcgga
     4501 aaataccgta ttatcgactt tccgctgagt aactgtatta attcaggaat tgatacagta
     4561 ggagtgctga ctcagtacca gccgctgagg ctgaatactc atatcggaat aggaattccg
     4621 tgggatttag acagaaatgt cggaggtgta tcgatacttc ctccttatga gaagagttct
     4681 aacagtgaat ggtacaccgg tacggcaaac gcgatttatc agaatcttga ttatatggag
     4741 acgtataatc cggattatgt tcttattctt tcaggcgacc atatttacaa aatggattac
     4801 gaagtgatgc ttgattttca caaagcgaat catgcggatg tgacgatcgc ggcaatgcct
     4861 gttccgattg aagaggcgag ccggttcggc atcgttgtta ctgatcatga gagcaggatt
     4921 aaagagtttg aagaaaaacc ggaaaaacca agcagtaatc tggcgtcgat gggtatttat
     4981 atctttagct ggccggtgtt aaaagaagct cttatcaagt tgaaagatca accgaattgt
     5041 gatttcggaa aacatattat tccgtattgt cacgacaaag gggacaggct ttttgcatat
     5101 gaatataacg gttattggaa agacgtagga acattgagtt cgtactggga agccaatatg
     5161 gaactgattg atattatccc tgaatttaat ttatatgaag aattctggaa gatctatacg
     5221 aacagcgcga atatcccacc tcagtatatt gcgcaggacg gagtggtaga tcggtgtatt
     5281 atcagcaacg gaacagaggt gtacggggaa ctgcataatt cggttttagg cggaagagtg
     5341 cgcgtcggaa agggaagtgt cgttcgtgat tcgattatta tgcagggagt aacgattgga
     5401 gagaactgtg tgatagacag ggcgatcata gcagaagata cggtaatcgg agataatgtt
     5461 gtgatcggaa tcggaagcga tgtgccgaac aagatgaaac cgaacattta cagtggagga
     5521 cttgctacga tcggcgagaa ctctgtgatt ccgtcaaacg tgcagatcgg aaaaaatact
     5581 gcgatcagcg gagtgacagc ggcggaagat tatgaggacg gcgtacttgc aagcggcgaa
     5641 accttgataa aggcaggtga gagagtatga gagcagtagg acttattttg gcaggaggaa
     5701 acagcaggaa gatgcgcgag ctgactgcaa aaagagcggt ggctgcgatg ccggtagcgg
     5761 gaagctatcg tgcgattgat tttgtactca gcaatatgac aaattctcat attcagaaag
     5821 ttgccgtgct gacacagtac aatgccagat cgctgaacga gcatctgaac tcatcaaaat
     5881 ggtgggattt cggaaggaaa cagggaggat tgttcgtgtt cacgcctacg attacggctg
     5941 acaacggata ttggtacaga ggaacggcag atgccattta ccagaatctg gatttcctga
     6001 aaaaatgtca tgaaccatat gtgatcatta cttctggtga tgcggtttat aagctggatt
     6061 ataataaagt gcttgagtat catattgcga agaaagcaga cataacggtt gtgtgcaagg
     6121 atcttgaacc gggagaagat gccacgagat ttggaacggt tcgcatgaat gaagaatcaa
     6181 ggatcgaaga gtttgaagaa aagccgatgg tggctcattc gcatacgatt tcaacaggaa
     6241 tttatgttct ccgcagaaga caactgatcg atctgatcga gcattgtgcg gaagatgaaa
     6301 gacatgattt tgtaaacgat attttgattc gttataaaaa tctgaaaaaa atatacggat
     6361 ataagatcaa cagttattgg aataatatct caacggtaga tgcgtattat aagacaaata
     6421 tggatttcct gaaaccggaa gtaagaaact actttttcag agagcttcca agcgtatatt
     6481 caaaggtaag cgatcagccg cctgcaaaat ataatccggg cgcagttgtg aaaaacagtc
     6541 ttatcggaag cggaagcatc gtaaacggaa cgattgaaaa ttccgttatc tttaaaaaag
     6601 tatttgtcgg taataattgt gtgatcaaaa attcaattat attgaatgat gtatatctgg
     6661 gagataatac atatatcgaa aattgtatcg tagagagcag ggatacaata agagcgaata
     6721 caagatacgt cggagaaaac ggcgtagaag ttgtagtag
//
LOCUS       DBMDXF010000100         6742 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2897_length_6742_cov_1.637555, whole genome shotgun sequence.
ACCESSION   DBMDXF010000100 DBMDXF010000000
VERSION     DBMDXF010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2897_length_6742_cov_1.637555"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="ACZPD4_05200"
     CDS             <1..282
                     /locus_tag="ACZPD4_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1378503.1"
                     /translation="QQEYIRAFGEALKKASADKELVTDLYQAIEPYMGTNMGKSRFVD
                     LTESVSEGRSVSRWTIPGEGIQGKEYDEYVVDDDTLYEKTVETFYVEKK"
     gene            299..1048
                     /gene="srtB"
                     /locus_tag="ACZPD4_05205"
     CDS             299..1048
                     /gene="srtB"
                     /locus_tag="ACZPD4_05205"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1378504.1"
                     /translation="MKKTSKIIGSVLIVAAVVCLGVGVYQFTKEQNAGKGYEDLSEEV
                     KKESEEDSKSKVDIPIDFAALKEKNSDVYAWINIPGTAIDYPILQRENDNTYYLDHTI
                     DHEEKTEGAIFTENYNNTDFEDPNTVIYGHDMRNGSMFKGLLDYRDKTFFDQNKEVLI
                     YTPDAIRHYKIFAAYPYDSRHLLRSFDFTDKEVYQQYLYRIFAIRDMNASIDTTAQVG
                     TDDKILTLSTCYANQPDMRFLVQAVLVSIEQ"
     gene            1092..1805
                     /locus_tag="ACZPD4_05210"
     CDS             1092..1805
                     /locus_tag="ACZPD4_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1378505.1"
                     /translation="MKKKIMGVILAATLIVSQAVTAFAGSKTSEVVLPNDFAAYYTVT
                     EGTREAFAEAEAVSPQTVDAIMEVNNGSMTLAQLVADMKELLKAEGAAGLKMTEEQVD
                     ALAADLGSRTFVTKFFDLEPVNGGIKDDNGDYLVTLSVPAISTAMTEVAFLHFSTDRA
                     AWEVVEASDVDYTNKEITASFKDLSPVAVIAKVDTSADNAIGTSPQTGRFPVSVLWIG
                     AAVILGATGVIVLRKRKEK"
     gene            2087..4027
                     /locus_tag="ACZPD4_05215"
     CDS             2087..4027
                     /locus_tag="ACZPD4_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1378506.1"
                     /translation="MRGKTTFHIRWKLFFYDVIVFAVTSVFLLVLYEGPVKLTQRGEL
                     QQMLLTFVCVFACRLVGKIYGQIWRYGGIQCYMRLVCTDCVAFLIYVILESVLPVAHI
                     SFPRLLALSTVNTMGALIMRMSYRYAYKCSNLETFSGKILAALLKIFAGIEVGETRDD
                     QKIKVAIIGAGNVGVTLAGELLANKMASYVPKCFVDIDKSKGGREIQGLPVLSENEAT
                     FQKLKKYGIQEIIFALPSMADERRTERYLYYKNAGYKVKMYDYPKMQMAGGKRSLREF
                     DIEELLFRAPRKLTNEKTDAYYNDKVILVTGGGGTIGSELCRQIVKMGPKKLIILDVY
                     ENGAYDLQQELRIAYGNEPGISVEIASITDSKAMERVFSKYHPQIVINAAAHKHVPLM
                     ENNCIEAINNNVFGTAVVVEMCEKYGAERFMMVSTDKAVNPTNVMGATKRMCEMIVQS
                     ASINGKVKYSATRFGNVLGSAGSVIPLFKRQIQNGGPITLTDKRIIRYFMTIPEASQL
                     VLESGVIAHNGELLVLDMGKPIKILDLAENMIHLSGANNIKIVETGLRPGEKLYEELL
                     VKTEELDKTENDLIFREKDKPLPTEAIREKLRMLKDVCENGDDEQAREMLRKVVPTFK
                     RPEEVNREVGDEVEPEDNSFLR"
     gene            4179..4805
                     /locus_tag="ACZPD4_05220"
     CDS             4179..4805
                     /locus_tag="ACZPD4_05220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1378507.1"
                     /translation="MKRKAYDGIYNQYIKRGIDFCLALVGLIIAAIPMAIVAVAIKAE
                     SKGPVIFKQDRIGYKGKVYKMYKFRSMCVGAEKGGVYSDNKDSRVTKVGKFIRATSID
                     ELPQLVNVLKGDMALIGFRSPLTYHPWPWSEYTEEQKKMFNVRPGITGWAQVNGRKTV
                     EWNKRIELNGWYAEHVSFWLDVKILFMTVFKVLTNADNENVGETVKQK"
     gene            4888..5730
                     /locus_tag="ACZPD4_05225"
     CDS             4888..5730
                     /locus_tag="ACZPD4_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HIR1378508.1"
                     /translation="MPLKLMYITNSEDVAHIAEEAGVDRIFVDMEYIGKTDRQGGMDT
                     VQSRHTADDVRRLAQCLNKAELFVRVNPIHEKTDKYSSSKEEIDEVIEAGADLLMLPY
                     FKTAEEVQTFVKYVNGRAKTVLLVETPEAAAIAEDLVQISGIDEMFIGLNDLSLGYGK
                     NFMFELLLDGTVEELCLKFRKGGIPYGFGGIASLGKGMLPSEYVIKEHYRLGSTCAIL
                     SRSFCRTDQVTDLKEIQKIFETELPKIRELEEECRVHSEYFWKNRQQVEAKIRQICEC
                     EDAK"
     gene            5727..>6159
                     /locus_tag="ACZPD4_05230"
     CDS             5727..>6159
                     /locus_tag="ACZPD4_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR1378509.1"
                     /translation="MNLLVTGAWQGAKDGIEQLKTLGHQVQFLQFEKDELPCSYEWVE
                     GVICNGLFLSHPIEQFTHLKYIQLTSAGFDRVDMQYVKKHNIEIHNARGVYSIPMAEF
                     AIFGVLELYKQGKFFQQNQAEKRWEKQRNLSELFGKQVCIAG"
     assembly_gap    6160..6169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6170..6666
                     /locus_tag="ACZPD4_05235"
     CDS             <6170..6666
                     /locus_tag="ACZPD4_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1378510.1"
                     /translation="GNVGTECAKRFSAFGCRITGVDLYPREDENYGKMVGIENLSEVL
                     SKSDIVILTLPLTKETRHLMNEEKFSQMKDRAILVNIARGAVVDTEALMREMPRLGGA
                     VLDVFEEEPLREDSPLWEMEKVIVTPHNSFVGDGNARRLAGVIYENIRRELSDKYTSF
                     KSNV"
BASE COUNT         2304 a         1085 c         1712 g         1631 t
ORIGIN      
        1 cagcaggaat atatccgagc attcggtgaa gcgttaaaaa aggcatctgc cgataaagaa
       61 ctcgttacgg atctgtatca ggcgattgaa ccgtatatgg gaacgaatat gggaaaaagt
      121 cgttttgtcg atctgacgga aagcgtgtct gaaggcagat cagtgagccg atggacgatt
      181 cccggagaag gaattcaagg taaagagtac gatgaatatg tcgtagacga tgacaccttg
      241 tatgaaaaaa cagttgagac attttatgta gagaaaaaat aaattgcaga acagacatat
      301 gaaaaagaca tctaagataa taggaagcgt tcttattgtc gccgcagtcg tctgtctcgg
      361 cgttggagtt tatcaattta cgaaagaaca aaacgccgga aaaggatatg aggatctttc
      421 ggaagaagta aaaaaagagt cggaagaaga ttcaaaatca aaagtcgata ttccgatcga
      481 ttttgcggcg ctaaaagaaa aaaattcgga tgtgtatgca tggatcaata ttccgggcac
      541 ggcgatagac tatccgattc ttcaaaggga gaatgacaac acatattatt tggatcacac
      601 gatcgatcat gaagaaaaga ccgaaggagc gatttttacg gagaattaca acaatactga
      661 ttttgaagat ccgaatacgg tcatttacgg gcatgacatg agaaacggtt cgatgtttaa
      721 aggactgctc gactatcggg ataaaacatt ttttgatcaa aacaaagaag tgctgatcta
      781 tacgccggat gcaatacgac attataaaat attcgctgcg tatccgtatg acagcagaca
      841 tttgcttaga agttttgact ttacagataa agaagtgtat cagcagtatt tataccgtat
      901 ctttgcaatc cgcgatatga atgcgagcat tgatacaaca gcgcaggtgg ggacggacga
      961 taagatcctg accttatcaa catgctatgc aaaccagccg gatatgaggt ttcttgttca
     1021 ggcagtgttg gtttccatag aacaataagg caaaagaaaa tcatttatca gagaaaggaa
     1081 gagtgaacaa tatgaaaaag aaaatcatgg gtgtaatttt ggcggcaacg cttatcgtat
     1141 cacaggcagt aacggcgttt gcaggaagta aaacgtcaga agttgtgctt ccgaatgatt
     1201 ttgcggctta ttataccgta acggaaggaa caagagaggc atttgcagag gcagaggcag
     1261 tttccccaca gacagttgac gcgatcatgg aagtaaataa cggaagcatg actcttgcac
     1321 agcttgttgc agatatgaaa gaactgctca aagcagaagg cgcagcagga ctgaagatga
     1381 cagaagaaca ggtcgacgct ctggcggcag atctgggttc cagaacgttt gtcacaaagt
     1441 tttttgacct tgagcctgta aacggcggga ttaaagatga caacggagac taccttgtaa
     1501 cattaagcgt gccggcaatt tccacagcta tgacagaagt agcgtttctt cacttcagca
     1561 cagaccgcgc ggcatgggaa gttgtggaag cgtcagacgt agattataca aacaaagaga
     1621 tcactgcgtc ctttaaagat ctttcgccgg ttgccgtgat cgcaaaagta gatacgtccg
     1681 cagacaatgc gatcggaacg tctccgcaga caggaagatt ccctgtttcc gtactttgga
     1741 tcggtgcagc agtaatcctt ggtgcgacag gcgttattgt gttaagaaaa cgcaaagaaa
     1801 aataaatatt gaatgtgcct tgaaaggctg acggtaagga atggctcgat gataagaaat
     1861 attttcgggc tattttcttg caaaaatcag gggaaaaacg gctattttcc ggtaatgtat
     1921 agtaacaaaa gaaccggata cgcaaaagac aaaaatttac gaaacagtaa aaagaaaaag
     1981 aaagaaatag cagttaaaga agtatgaaag ataaactaga gggacagcag ttaaaaacat
     2041 caaaacatga aagatggatt tggagagata acagaggaaa ttacagatga gaggaaagac
     2101 tacatttcat ataagatgga aattattttt ttacgatgtg atcgtatttg cagtgacgtc
     2161 tgtatttctg cttgtattat atgaaggacc ggtaaaattg acgcagagag gggaattgca
     2221 gcagatgctt cttacatttg tatgcgtatt tgcctgccgg ctggtcggaa agatatatgg
     2281 gcagatatgg agatacgggg gaattcaatg ctacatgcgt ttagtttgta cggattgtgt
     2341 ggcattttta atatatgtga ttttggaatc tgttcttccg gtcgctcata tctcatttcc
     2401 gcgccttctg gcgttgagta cggtaaacac catgggagcg ctgatcatga gaatgtctta
     2461 ccgttatgcg tataagtgca gcaacctgga aactttttca ggtaaaattt tagccgcact
     2521 gctgaaaata tttgccggaa ttgaagtggg ggaaacaaga gacgatcaga agatcaaagt
     2581 ggcgatcata ggggcaggaa atgtcggagt tactctggca ggggaactgt tggcaaacaa
     2641 gatggcatcc tatgtgccga aatgttttgt ggatatcgat aaatcaaaag gcggacgaga
     2701 gatacaggga cttcccgtat tatctgaaaa cgaggcgacg tttcagaaac tgaaaaaata
     2761 cggaatacag gaaattatct ttgcgctgcc gtctatggcg gatgaaagaa gaacagagcg
     2821 atatctttat tataaaaatg ccggatataa agtgaagatg tacgattatc ctaagatgca
     2881 gatggcaggc ggaaagagat cgcttcgaga atttgatata gaagaactgc tatttagagc
     2941 tccgagaaag cttacgaacg aaaagacaga tgcttattat aatgataaag taatcctcgt
     3001 cacaggagga ggcgggacga tcggatcgga actgtgcaga cagattgtaa aaatggggcc
     3061 gaaaaaattg atcatccttg acgtttatga aaacggagct tacgatcttc aacaggagtt
     3121 aagaatcgca tatggcaacg aaccgggcat cagtgtggaa atcgcttcga ttacagattc
     3181 aaaagcaatg gagcgtgtat tttcaaaata tcatccccag atcgttatta atgcggctgc
     3241 ccataaacat gttccactta tggaaaataa ctgcatcgaa gcgatcaata acaacgtgtt
     3301 cggaacggcg gttgtcgttg agatgtgcga gaaatacggc gcagaacgct ttatgatggt
     3361 ttcgacagat aaagcggtta atccgactaa cgtgatgggt gcaacgaaga gaatgtgcga
     3421 aatgatcgtg caaagcgcca gcattaacgg aaaagtaaaa tacagcgcga caagattcgg
     3481 aaacgtatta ggaagcgcag gaagtgtaat tcctctgttt aaacgtcaga tccaaaacgg
     3541 tggaccgatc acacttacag ataaaaggat catccgctac tttatgacga ttccggaggc
     3601 gtctcagctc gtgttggaga gcggcgtgat tgctcacaat ggcgaactgc ttgttctcga
     3661 catgggaaaa ccgatcaaga tactggatct tgcagaaaat atgatccatc tgtcaggagc
     3721 aaataatatt aagatcgtag agacaggact ccgtccggga gaaaagctgt atgaagaact
     3781 tcttgttaag acagaagaac ttgataagac cgaaaacgat ctcatcttta gagaaaaaga
     3841 taagccattg ccgacagaag caatccgtga aaaattaaga atgctcaaag acgtctgtga
     3901 aaacggagac gatgagcagg caagagaaat gctgcgcaaa gttgtgccga catttaaaag
     3961 accggaagaa gtgaaccggg aagtaggcga tgaagtagag ccggaggata acagcttcct
     4021 tcggtgaaga atgaaagcct gaaattcttg gataaggtga agagatacga tgaaaagtgc
     4081 ggatcggcat tggcagataa agtgtgcggc aggagtaatc aaaaggaaaa taatataata
     4141 aacataagta aaaagaaaga atctacagga gagagaaaat gaagaggaaa gcttatgacg
     4201 ggatttataa tcaatacatt aagcggggaa ttgatttttg tcttgcattg gtcggtctga
     4261 tcattgcagc aattccgatg gcaatcgttg cagttgctat taaagcggaa tcaaaaggtc
     4321 ctgtgatctt taaacaggat cgaatcggtt ataagggaaa agtatataaa atgtataaat
     4381 ttcgcagcat gtgtgtcgga gcagaaaaag gcggcgttta ttcggataat aaagacagcc
     4441 gcgtgacaaa agtcggaaaa ttcatacgcg cgacaagcat tgatgagctt cctcagcttg
     4501 taaatgttct gaaaggcgat atggcgctga tcggcttccg cagtccgctt acatatcatc
     4561 cgtggccatg gagtgaatat acagaggaac agaagaagat gtttaacgta cgtccgggaa
     4621 ttacaggctg ggcgcaggtg aacggaagaa aaacagttga atggaataaa cgtattgaat
     4681 tgaacggctg gtatgcagag catgtttcct tttggctgga tgtaaagatt ttatttatga
     4741 cagtatttaa agtacttaca aatgccgaca acgaaaacgt cggagaaaca gtgaaacaaa
     4801 aatagaaaca gatattgcta tagaaaatga cggaagatca aataaaacga tcaaaagaaa
     4861 agcagacaaa acagagggga tgatcagatg ccattaaaat tgatgtatat tacaaattca
     4921 gaagatgtcg ctcatatcgc agaagaagca ggagttgacc gtatttttgt cgatatggaa
     4981 tatatcggca aaaccgatag gcagggcggg atggacacag tgcagtccag acatacggcg
     5041 gatgatgtga ggagacttgc acagtgctta aataaagcgg aactatttgt aagagtgaat
     5101 ccgattcatg aaaaaacaga taaatatagt tcgtcgaaag aagaaatcga cgaagtgatc
     5161 gaagcaggtg cagatctttt aatgctgcct tactttaaaa cggcagagga agttcagaca
     5221 tttgtaaaat atgtaaatgg cagagcaaaa acagttcttt tggttgaaac acccgaagcg
     5281 gcggccatag cagaggatct ggtacaaatt tcggggattg acgagatgtt tatcggatta
     5341 aatgatttat cattaggtta tggtaaaaat tttatgtttg aactgctgct tgacggtacg
     5401 gttgaagaac tctgtctgaa attcagaaaa ggcggaatcc cttatggatt tggcggaatt
     5461 gcttcattag ggaaaggaat gcttccttcg gaatatgtga tcaaagaaca ttatcgtctt
     5521 ggttctacat gtgcgatttt gtcacggagt ttctgcagga cagatcaagt gacagactta
     5581 aaagagattc agaagatttt tgaaactgaa ttgccgaaaa tacgggaact tgaagaagaa
     5641 tgcagagtac atagtgaata tttttggaaa aacagacaac aggttgaggc aaaaataaga
     5701 cagatctgtg aatgtgagga tgcaaaatga atttattagt aacaggagca tggcaagggg
     5761 caaaagacgg gattgaacaa ttaaaaacgt tggggcatca ggtacaattc ttgcaatttg
     5821 aaaaagatga gcttccgtgt tcatatgaat gggttgaagg agttatatgc aacggactat
     5881 ttctttcgca tccgatcgag caatttactc atttaaaata tattcaactg acaagcgccg
     5941 gatttgacag agttgatatg cagtatgtaa aaaaacataa tattgagata cataatgcaa
     6001 gaggggtata tagtatccca atggcagagt tcgcaatatt cggagtatta gaactgtata
     6061 aacaaggcaa attctttcag cagaatcagg cggaaaaacg gtgggagaaa caaagaaatc
     6121 tgtcagaatt gttcggaaag caagtatgca ttgcaggatn nnnnnnnnng cggcaatgtc
     6181 ggaacagaat gtgcaaaacg tttctccgct ttcggatgca gaataacggg cgtagatctg
     6241 tatccgagag aagatgaaaa ctatgggaaa atggttggaa tcgagaacct gtccgaagtt
     6301 ctctccaaaa gtgatattgt catattgact ttgccgctga ctaaggagac aaggcatttg
     6361 atgaacgaag aaaaattcag ccaaatgaaa gacagggcga ttcttgtaaa tattgcaaga
     6421 ggcgctgtcg tagatacaga agcgttgatg cgggagatgc caagacttgg cggagcggtg
     6481 ctggatgtgt ttgaagaaga accgctgagg gaagatagtc cgttatggga gatggagaaa
     6541 gttatagtga caccgcataa cagctttgtc ggggatggaa atgcccggag actggcggga
     6601 gtgatctatg aaaatataag gagagaatta agtgataaat atacaagttt taaaagtaat
     6661 gtttaataaa atggattcat gtataaaaat gaaccggggg ggggtaaatc ttttttacag
     6721 aatgcgttat ggaatgggaa tt
//
LOCUS       DBMDXF010000101         6690 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2923_length_6690_cov_2.211109, whole genome shotgun sequence.
ACCESSION   DBMDXF010000101 DBMDXF010000000
VERSION     DBMDXF010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_6690_cov_2.211109"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="ACZPD4_05240"
     CDS             complement(<1..500)
                     /locus_tag="ACZPD4_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1378511.1"
                     /translation="MKVYRTDEIRNVVLLGHGGSGKTSLVEAMLYVSGVTNRMGKVTE
                     SNTVSDFDKEEQKRGFSISTSLVPIEWEKAKINILDTPGYFDFVGEVEEAVSAADAAV
                     IVVSGKAGVQVGTEKAWELCDKYNLPRMIYVTEMDVDDASFRQVVEDLTTRYGKKIAP
                     HFQPIR"
     gene            811..1920
                     /locus_tag="ACZPD4_05245"
     CDS             811..1920
                     /locus_tag="ACZPD4_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR1378512.1"
                     /translation="MFSDITPAKIGFIGLGLIGGSVAKAIRTYFPDYELIAFDKNKET
                     LALAVQENIIDTSCSAIDDNFKGCSYIFLCAPVSYNTACLTQLKSLIDPDCILTDVGS
                     VKTSIHEEIISLGMEENFIGGHPMAGSEKNGFANSKAHLIENAYYILTPSAKVSEEKI
                     NAYRNFVSSLKALPIVLDYRQHDCITGTISHLPHIIASTLVNFVRDTDTKEELMKALA
                     AGGFKDITRIASSSPVMWQQICLKNGKNISHILGQYIEALIQAKLSIDTEDENALYTL
                     FESSRDYRNSMPNTSAGPIKKQFAVYCDIIDETGGIATIATILASNNISIKNIGIIHN
                     REFEEGVLRIEFYDESSSSKASALLKKYRYIVYEV"
     gene            1956..3245
                     /gene="aroA"
                     /locus_tag="ACZPD4_05250"
     CDS             1956..3245
                     /gene="aroA"
                     /locus_tag="ACZPD4_05250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1378513.1"
                     /translation="MELAGIQGLKGTVSVPGDKSISHRCIMFGSIANGTTEIHNFLKG
                     ADCLATIRCFQSMGINIEETDHTITVHGKGLHGLSAPLNILDVGNSGTTTRLLSGILA
                     GQKFESKLSGDESLNSRPMKRIIEPLTMMGANISSILRNGCAPLYIAPGNLHGIHYDS
                     PVSSAQVKSCILLAGLYAEGETSVTEPSLSRNHTELMLKEFGADIRTLHSLSGTEATA
                     YIKPYPKLYGQKIVVPGDISSAAYFIAAGLIVPDSEILIEHVGINPTRSGILKVCEDM
                     GGDITLLNERCEAGEKIADILVRTSSLHGITIEGDIIPTLIDEIPIIAVMAAVADGTT
                     VIKDAAELKVKETDRIETVTDNLKAMGCNVTPTEDGMIITGGTLHGASIHTLLDHRIA
                     MAFSIAALVADGTTKILDSKCVDVSYPTFYDTFEQLL"
     gene            3375..3578
                     /locus_tag="ACZPD4_05255"
     CDS             3375..3578
                     /locus_tag="ACZPD4_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378514.1"
                     /translation="MIKKLIKKEKKQLIALFISLISLIIFSLLDNKIDKIFLFFFIGD
                     LIIIFVDVCLELKKYWKNKKDKK"
     gene            complement(3750..4616)
                     /locus_tag="ACZPD4_05260"
                     /pseudo
     CDS             complement(3750..4616)
                     /locus_tag="ACZPD4_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427792.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
     gene            complement(5199..5519)
                     /locus_tag="ACZPD4_05265"
     CDS             complement(5199..5519)
                     /locus_tag="ACZPD4_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378515.1"
                     /translation="MGRKAFDNSIDYKEIGTRIRIMRLKLAVSQTECASKLGISQTHL
                     SNIEHGRSGITLQMLCRVCDILQVSLDYLVRGAEKNDEKKHSEITIDEVVRVIKMLKG
                     IEEK"
     gene            complement(5523..6338)
                     /locus_tag="ACZPD4_05270"
     CDS             complement(5523..6338)
                     /locus_tag="ACZPD4_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HIR1378516.1"
                     /translation="MNDADVKADKDAIKAAGALATQKDLEAVNRDAETAKETAEAAEK
                     EAADAHSKATGAYLEAKQAQEKNTEQDKTLSEQGQKISENSTAIEQNTADIETNKQGI
                     VTNKENIEKNTQAITTERTERIQEVQRLDGRIDGLSNRIDELDGRIDKVGALAVAMAG
                     LHPLEYDPDAPTQFSMAAGTYSGESAIAAGLFHNPNKDVLLSVGFSISGSEKAANVGA
                     TFRFGRSSESKARKVIEDKQREQELAAQAAAARQKTVSYRVEQILAEDTAKAE"
BASE COUNT         1801 a         1661 c         1243 g         1985 t
ORIGIN      
        1 tcgcggatcg gctggaaatg cggcgcgatc tttttgccgt aacgggtagt cagatcttct
       61 actacctgac ggaaacttgc atcgtcaacg tccatctctg tcacatagat cattcgcgga
      121 aggttatact tatcacacag ctcccaggct ttctccgtgc ctacctgaac tcccgctttg
      181 ccggaaacaa cgatgaccgc tgcgtccgcc gcgcttaccg cttcttccac ttctcccaca
      241 aaatcaaaat atcccggtgt atcaagaatg ttgatcttcg ccttttccca ttcgatcgga
      301 acaagacttg tgctgatcga aaaaccacgt ttctgttctt ctttatcaaa atcactgacc
      361 gtgttagatt ctgtcacttt ccccattcgg ttcgtaacgc ctgatacata cagcatcgct
      421 tcaacaagac tcgtctttcc gcttccgccg tgtccgagca ataccacatt tctaatttcg
      481 tctgttctgt aaactttcat gctgccgcct ccttgatatg tagttatttt gcatgtattt
      541 ctttcctctc gccatgcaca tcataggtat attgtactaa atttttttat aaattacaac
      601 tattttatat atttttcaca tatttcttat gcagttcaac attgcagttc tcttcctgtt
      661 tctgaggaaa acagtgtcgt catacacgat aagcatacgg tgcggtttta cgaaccggat
      721 cggcttaact attaccatta ctttgtttac ttttacaagt cagcgtgtta aaatacctct
      781 atattataat aaggaaagga ttttactttt atgttttcag acatcactcc tgcaaaaatc
      841 ggctttatcg ggctcggact tatcggcggt tctgtcgcaa aggcgatccg cacatacttc
      901 cccgattatg agctgattgc attcgataaa aataaagaaa ctctcgctct cgcagtccag
      961 gaaaacatca tcgacacttc ctgttctgcg atcgatgaca attttaaagg ctgcagctat
     1021 attttcttat gcgcccccgt ttcatacaac accgcctgcc ttacccagct aaaatctctg
     1081 atcgatccag actgtatttt aacagatgta ggaagcgtta aaacttcaat ccacgaagaa
     1141 ataatctcgc ttggtatgga agaaaatttt ataggcggtc atccgatggc aggttcggag
     1201 aaaaacggat tcgccaactc aaaagctcat ttgatcgaga acgcttacta tattctcacc
     1261 ccttcagcta aagtatccga agaaaaaatc aatgcttacc ggaactttgt ttcttcttta
     1321 aaagcgctgc ctattgtcct cgactacagg cagcacgact gcataaccgg aacgatcagc
     1381 catctgcctc acatcattgc ttctacactt gtaaacttcg tccgggatac cgatacgaaa
     1441 gaagaactga tgaaagcgct tgccgccggc ggtttcaaag acattacgag gatcgcatcc
     1501 tcgtccccgg ttatgtggca gcaaatctgt ctgaaaaacg gaaaaaatat ctcgcatatt
     1561 ttaggacagt atatcgaagc actcatacaa gctaaacttt ctatagatac ggaagatgaa
     1621 aatgcgcttt atactttatt tgaatcttcc cgcgattatc gtaattctat gcctaacacc
     1681 tcggcagggc cgatcaaaaa acagtttgcc gtatactgtg atattattga tgaaaccgga
     1741 ggaatcgcga cgatcgcaac tatattggca agcaataata tcagtattaa aaatatcggc
     1801 attatccaca accgcgaatt tgaagaaggc gtactccgaa tcgaatttta tgatgaatcc
     1861 tcctcttcca aagcttctgc gctgctgaaa aaataccgat atatcgtata cgaagtctaa
     1921 gaaagaaaca ataatttaaa caggaggaaa aagcaatgga attagccggt attcaaggat
     1981 taaagggaac agtttccgtt cccggagata aatcaatttc ccaccgatgc atcatgttcg
     2041 gatcgattgc aaacggaacg acggaaatcc acaattttct gaaaggcgcg gactgtcttg
     2101 ccacgatccg ctgctttcag tctatgggca ttaatataga agaaacagat catacgatca
     2161 cggtccacgg gaaaggatta cacggactat ccgctccctt aaatatcctc gacgtcggaa
     2221 acagcggcac tacaacccgc cttttgtctg gcattctcgc gggacagaag tttgaatcaa
     2281 aactttcggg agacgagtct ttaaactccc gccctatgaa acggatcatc gaaccgctta
     2341 caatgatggg cgcaaatatt tcaagtattc tcagaaacgg atgcgctccg ctgtacatcg
     2401 ctccgggaaa tctgcacgga atccactacg attcccccgt ctcttccgca caggtgaaat
     2461 catgtatcct gcttgccgga ttgtacgcag aaggagaaac ttcggttaca gaaccgtcgc
     2521 tttccaggaa ccacactgaa ctgatgctga aagaattcgg cgctgacata cggactcttc
     2581 actcgctgtc aggaacagaa gcaaccgcat atattaaacc gtacccaaaa ctatacggtc
     2641 aaaaaatcgt tgttccggga gatatttctt ctgcggcata ttttatcgca gcaggactga
     2701 tcgttcccga ttcggaaata ttgatcgaac atgtggggat caacccgacc agatccggta
     2761 ttttgaaagt atgcgaagat atgggcggcg atatcacact tcttaacgaa cgatgtgagg
     2821 ccggcgaaaa aattgccgac atcctcgtcc gcacgagcag tctgcacgga attacgatcg
     2881 aaggcgatat tatcccgaca ttgatcgacg aaattccgat catcgcagtc atggctgccg
     2941 tcgcagacgg aacaactgtc atcaaagacg ccgctgaact gaaagtaaaa gaaactgacc
     3001 gcatcgaaac tgtgacagat aatttaaaag cgatgggatg taacgtcacc cccacagaag
     3061 acggaatgat catcacaggc ggaacacttc acggcgcttc gatccacacc cttctcgatc
     3121 acaggattgc aatggcgttc agcatcgcgg ctcttgtcgc tgacggaaca acgaaaatcc
     3181 ttgacagtaa atgtgtggac gtatcgtatc ctacttttta tgatacattt gaacaattat
     3241 tataatcgta tccgaattca tataacttca aaaaagttat ttaacttcct tattttcaat
     3301 ggtaaaaatc acgtttttgc tatattggaa ttgaataata tcagtttctt aatagaaaaa
     3361 ggagggtaca atttatgatt aaaaagctaa taaaaaaaga aaagaagcag cttatcgccc
     3421 tattcatatc acttatatct cttattatat tttctttgct ggacaataaa atagataaaa
     3481 tttttctatt tttctttatc ggggatttaa ttattatatt tgtcgacgtt tgtttggaac
     3541 ttaaaaaata ttggaaaaat aaaaaagata aaaaatagca ttttaaaaga gacattcatc
     3601 gatcgcactg ttttcattcg atcagaaaca gtaagatcat cgatgtctct tttttgtgac
     3661 ggcaataaac taaggtccgg gcaaccccgg acctcagcga ctgtttacaa tcgcgaaatg
     3721 tgccttttgg cacttcagac gattgctttt tacagcatct cttttcttaa ttcttctccg
     3781 ctttttgcgc ttaaatatgc cggagcgata tcgaacactg tcttgcatcc gctctgacct
     3841 tccgcagcaa gtctgtaagc tgcgcgggca tatgcaacga tcacggacga tgtaaactca
     3901 ggattagagt ctaattttaa gctgtactcg atcaaatggc tgttttctcc gtcccagcct
     3961 gtctttccgc ttctaagtac gaaaccgccg tgcgggattc cgctgtgatc gcgttttaat
     4021 tcttcttcac ttataaaatg aaccgtcgta tcataatcgg caaaatagtt cggcatcgat
     4081 ttgatctccg cttctacttt tgccgcgtcc gcgccctctt caaggacaac aaaacactct
     4141 cttgtatgtt tctgacgtgt cgtaagttcc gggttttctc cgtttcgcac tgcctcaaga
     4201 gctgcttctg acggaatcgt gtactgtttt ccgtctttta cgccttctac tcttctgatc
     4261 gcatctgaat gaccctggct tactcctttt ccccagaatg tataatcttt tccgtcagga
     4321 aggatcgcgt ttgcatacaa tctgtttaaa gagaacatac ccggatccca gcctacggaa
     4381 ataattccga cttttccact ctccttcgcc gcagcatcca cattcgcaaa atgctccgga
     4441 atgcgggcat gtgtgtcaaa actgtcaatt acattaaaca gctttgcaaa ctccggcgtc
     4501 tgtttcggaa gatctgtcgc gcttcctccg caaagaatca atacgtcgat cttatctttc
     4561 cagtctgcgg cgtcatccac actgcatacc ggaactcctt ctgtcaaaat tgccactaag
     4621 cttgagaaat cattttccgt aaatttcctg tgagcctcaa tagcatacac ttcatcacaa
     4681 aaaaccttgc ctatcgcttc atcagattgc ccagaatcaa atagtctaat cagatcattt
     4741 acccatccct gagcaatttc acttctgcca gtagaggtga tccctcctcc gctaccatcc
     4801 acagcatctc tgtggatagc ataatcaagc gcataagtca tagaatcgcg ataagtaact
     4861 ccgtggctat gcaaaaatac tgagccacgg ataacagcgc ctggaataac atgttcccat
     4921 tctcctctgc tatccaataa atcgctggca gaacctccct ccttataaat ttttacacga
     4981 ccacaccttt caccatggtc acaatttgga tcagtacaaa taatacacat aaccaatacc
     5041 tccttatatt ttaatctgct tttagaataa cattaacctg atttacaatg gtggaaaatg
     5101 ccgccttccg caaaataata atttaaatac tcagacaaaa aaagagcaaa ccaaaatggt
     5161 ttgctctttt tttatgataa acttgctgtc tgccggactt atttttcttc tatgcccttc
     5221 agcattttta tgacccgcac aacttcgtcg atcgtgatct cgctgtgctt tttttcgtcg
     5281 tttttttctg cgcctctgac cagataatcc aagcttacct gcaggatatc acagacccgg
     5341 cacagcatct gcaaagtgat cccgctgcgg ccatgctcga tattactcag gtgtgtctgc
     5401 gaaataccca gtttagaggc gcattcagtc tgtgaaaccg ccagcttcag ccgcatgatc
     5461 ctgatcctgg tgccgatctc tttataatcg atgctgttat caaatgcttt tctgcccatc
     5521 tgctattcag ctttcgctgt atcttcagcc aaaatctgtt ctacacgata ggaaacggtt
     5581 ttctgtctgg cggctgcggc ctgggcagcc agctcctgct cgcgttgttt gtcttctatc
     5641 actttacggg cttttgattc cgagcttctg ccaaagcgga aggtagcgcc gacatttgct
     5701 gccttctcac tgccgctgat cgaaaaaccc acgctcaaaa gtacgtcttt attagggttg
     5761 tggaacagac ctgccgcaat agcgctttca ccgctgtaag tacctgccgc catcgagaat
     5821 tgagtcgggg catccgggtc atattccagc ggatgcaggc cggccatagc gactgccaaa
     5881 gctccgactt tatcaatgcg tccatccagc tcgtcgatgc ggttgcttaa gccgtcgatc
     5941 cgcccgtcta aacgctgtac ttcctggatg cgttctgtcc gttcggtagt gattgcctgc
     6001 gtattttttt caatattttc tttgttggtc acgatgcctt gtttattagt ttcaatatcc
     6061 gctgtgtttt gttctatcgc cgtagaattt tccgaaatct tttgaccctg ctcacttaaa
     6121 gtcttatctt gttcagtatt tttttcttgg gcctgtttgg cctctaagta tgctccagta
     6181 gcctttgaat gtgcgtcagc agcttccttc tctgctgcct ctgcagtctc ttttgcagtt
     6241 tccgcatccc tatttactgc ttccaaatct ttttgtgttg ctaaggcgcc ggcagctttt
     6301 atagcatctt tatccgcttt aacatccgca tcattcatga cacctaaacc tgcatgtatt
     6361 tccaaaccag taccaatcgc agccgcatag ctttttaaac aaaagtcccc cccatatgta
     6421 ctccgcccat tatcaggctc aaaacgacag cctttactaa aaccttattc atccttttca
     6481 tattctttta cccccttgag atttatttag catctttcta aaacatgcta aatttcaccg
     6541 tatttattca acatccagca aaaaaatcct gctctctttt tgcatattta agcaattaca
     6601 agggataaac tggctatttt gtgtatatta atatcatatt ttataatatt aattcatttc
     6661 atttcttcgt ctgccagcat ttctattttt
//
LOCUS       DBMDXF010000102         6564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_2993_length_6564_cov_1.984991, whole genome shotgun sequence.
ACCESSION   DBMDXF010000102 DBMDXF010000000
VERSION     DBMDXF010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_6564_cov_1.984991"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="ACZPD4_05275"
     CDS             <1..216
                     /locus_tag="ACZPD4_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1378517.1"
                     /translation="YDQNQGYQTHVHTFGFDTWWKKLLLILGIIGIIVLVVSIVGGII
                     SFLAPILIPLVIIMLIIRLIGGSGRRW"
     gene            599..856
                     /locus_tag="ACZPD4_05280"
     CDS             599..856
                     /locus_tag="ACZPD4_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378518.1"
                     /translation="MAKNYSNANNSDKNAQSKNGAFSSYSESQNSSKNCDKNTTKNAS
                     RNASKNANKNADKNSAKSAMSYGGNKNCGKIRDAFDESDKY"
     gene            1019..2188
                     /locus_tag="ACZPD4_05285"
     CDS             1019..2188
                     /locus_tag="ACZPD4_05285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1378519.1"
                     /translation="MDKIEWIAPCHFGLESVLKREVEQLGYEIVQVEDGRVTFRGGAE
                     AVCRANIFLRTAERVLMKVGSFQAKTFDELFEKTKELPWENYIPKDGKFWVAKAASVK
                     SKLFSPSDIQSIMKKAMVERLRGKYGIKWFEETGASYPVRVFLMKDTVTVGLDTTGVS
                     LHKRGYRPVAGKAPIAENLAAALIMLTPWKKDRILVDPFCGSGTFPIEAAMIAANIAP
                     GMNRSFTAETWTNIIAKKSWYEAIDEATDMIDDDIETDIQGYDADASVLRAARRNAEE
                     AGVGHLIHFQQREVKDLSHPKKYGFIITNPPYGERLEEKEALPKLYKEFGDAFKNLDS
                     WSAYMITSYEEAERYFGRKADKNRKIYNGMLRTYFYQFQGPRPPRKTVREQGMAL"
     gene            2185..2799
                     /gene="rdgB"
                     /locus_tag="ACZPD4_05290"
     CDS             2185..2799
                     /gene="rdgB"
                     /locus_tag="ACZPD4_05290"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1378520.1"
                     /translation="MKRRIVFATGNENKMKEIRMILSDLNTEIVSMKEAGVDIDIVED
                     GMSFEENAEIKARSVARILTNDIVLADDSGLEIDYLDKAPGIYSARFAGEDTSYDIKN
                     RIFLDRLEGVPDEERTARFVCAVAAVFPDGSVDVVRETIEGRIAHESAGTHGFGYDPI
                     FYLPEYGCTTAQLSPEQKNEVSHRGKALCAIKRKLKGKIGDDLL"
     gene            2796..3278
                     /locus_tag="ACZPD4_05295"
     CDS             2796..3278
                     /locus_tag="ACZPD4_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1378521.1"
                     /translation="MKVLIVSDTHGRHTGLDRVLEKEGKIDLFIHLGDIEGGESYINS
                     VVECEKHMVRGNNDFFSDLPREEEIDIGGYKAFITHGHPYYVSLDSEYIREEGAARKV
                     DIVMFGHTHKPYFEQKDGITVLNPGSLAYPRQEGRKGSYMIMEIDQEGKAHFNQKYLD
                     "
     gene            3442..3516
                     /locus_tag="ACZPD4_05300"
     tRNA            3442..3516
                     /locus_tag="ACZPD4_05300"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3477..3479,aa:Pro,seq:tgg)
     gene            3525..3595
                     /locus_tag="ACZPD4_05305"
     tRNA            3525..3595
                     /locus_tag="ACZPD4_05305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3557..3559,aa:Gly,seq:tcc)
     gene            3642..3715
                     /locus_tag="ACZPD4_05310"
     tRNA            3642..3715
                     /locus_tag="ACZPD4_05310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Arg,seq:tct)
     gene            3721..3794
                     /locus_tag="ACZPD4_05315"
     tRNA            3721..3794
                     /locus_tag="ACZPD4_05315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3755..3757,aa:His,seq:gtg)
     gene            3847..3918
                     /locus_tag="ACZPD4_05320"
     tRNA            3847..3918
                     /locus_tag="ACZPD4_05320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3879..3881,aa:Gln,seq:ttg)
     gene            3949..4021
                     /locus_tag="ACZPD4_05325"
     tRNA            3949..4021
                     /locus_tag="ACZPD4_05325"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3982..3984,aa:Lys,seq:ttt)
     gene            4161..4240
                     /locus_tag="ACZPD4_05330"
     tRNA            4161..4240
                     /locus_tag="ACZPD4_05330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4195..4197,aa:Leu,seq:tag)
     gene            4468..4695
                     /locus_tag="ACZPD4_05335"
     CDS             4468..4695
                     /locus_tag="ACZPD4_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378522.1"
                     /translation="MEALGVECTLESALNMAMIKEGLAQKTAFEEAYYGLNGTVFVDI
                     ENANIRTAFHKEQIDEYVKKMREVLKNHEKG"
     gene            4682..4855
                     /locus_tag="ACZPD4_05340"
     CDS             4682..4855
                     /locus_tag="ACZPD4_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378523.1"
                     /translation="MKKVDCPLMDECVDDAICFDIHMVVEGCAPKFTAPKKAVQTPDF
                     RKICMNCPNHRDD"
     gene            complement(5159..>6564)
                     /locus_tag="ACZPD4_05345"
     CDS             complement(5159..>6564)
                     /locus_tag="ACZPD4_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 95 catalytic
                     domain-containing protein"
                     /protein_id="HIR1378524.1"
                     /translation="SKLNSLITKAEQELNGNYTNDSLYQLKKVLSDAKKTADDENVKQ
                     PAVDQMTEKLQAALNSLEQGGFSETSIPAGDLQGSGKWIQAGDFKATEADDAGPLTYK
                     FNGNSIQVSTVKGDDHGVIRVTILDQNEKQICREEIDTYAEKRIEGAELIQKDFPEGS
                     YTIQFERVGKSPNGSPEKRGWVEVGTLKIRTEKKEIVDRSQLEEELTICDGLNKDKYT
                     EESWNKLQKAVNSARELLNKKDEETCTSEMNDKANEVRNARNSLQEIKVDTSALNDAL
                     KAAKSISREGYTEESFEALTQSILEAEALLNGTYTQKEVDEKTAVLIQRMKDLRADKT
                     LLQEKYNKIKDMTQGNATDASWNDFTRLRELANAILTDPNATPAEVSDILNQLNTFEF
                     AYKDDPDEGGNKDPQEPLEPQNPQNPNSGNQGNTNTSHADTSVKTGDSTPLLLPLATM
                     CSIIGLLYIMHKKRKLG"
BASE COUNT         1942 a         1154 c         1636 g         1832 t
ORIGIN      
        1 tatgatcaga atcagggata tcagacacat gtgcacacat ttggctttga tacctggtgg
       61 aaaaagctgt tgctgatact tggaattatt gggattatcg tgcttgtagt atcgattgtt
      121 ggaggaatta taagctttct ggcaccgatt ttgattccgc tagtgatcat tatgctgatt
      181 atcagactga ttggcggaag cggccggcga tggtaagcga gacaggcagc catataaata
      241 tgatttatat atagaaaata tatgaaataa gaaacagata ttccgacggg gttaaaatca
      301 agcctgccag aatatctgtt tttgtttgga aaaatagggc aaaataaaag actgataggg
      361 gagctatcag tcttttgagg ggagtataaa taattaataa ggggttgtag aaaataacct
      421 ttctacaaga aacagtataa tataggaaaa tggaaaaagc aagcaaaaaa ttaagaaatt
      481 gaacaaaaat atttgaaaaa tttaataaaa tacaaagaaa aacacaaata aaaagaggaa
      541 taaaaaaata ataaggagaa atactagttt tgaatcaaaa aaacaggagg aaaatacaat
      601 ggcaaaaaac tacagtaatg caaacaattc agacaaaaac gcacagtcaa aaaacggagc
      661 tttctcttct tattcagagt cacagaacag cagcaaaaat tgcgataaga acacaacaaa
      721 aaatgcaagc agaaatgcaa gtaaaaacgc aaacaaaaat gcagataaaa acagcgcaaa
      781 gtctgctatg agctacggcg gaaataaaaa ctgtggtaag atcagagacg cgtttgacga
      841 atctgacaaa tactaaagat taggtgaaaa acatctcgta tttacagaat acgatgtgaa
      901 atgttttatt cggtaaaata atagtaaaac aatggcaggg cggagtaaac ttcaacttta
      961 ctctgccctg tttttatagt aatattgtag gaagaaaaag gtaaggacgg ataagttcat
     1021 ggataaaata gaatggatag ccccttgtca ctttggtttg gaatcggtac tcaaacgaga
     1081 ggttgaacaa ctcggatacg agatcgtaca ggtggaagac ggcagggtta cttttcgcgg
     1141 aggcgcggag gctgtttgca gggcaaacat atttttgcgg actgcggagc gtgtattgat
     1201 gaaagtcggc agttttcagg caaagacatt tgacgagctg tttgagaaaa cgaaggagct
     1261 gccgtgggaa aattacatac cgaaagacgg aaagttctgg gtggcaaaag cggcatcggt
     1321 aaaaagcaag ctgtttagtc catctgatat tcagtcgatc atgaagaagg ctatggtgga
     1381 aagactgcgg ggcaaatacg ggatcaagtg gtttgaagaa acaggcgcgt cttacccggt
     1441 gcgcgtgttt cttatgaaag acacggtgac ggtagggctt gatacgaccg gagtatcgct
     1501 tcataagaga ggatatcgcc cggtggcggg aaaagcgccg atcgcagaga atctggcagc
     1561 ggcgcttatt atgctcactc cgtggaaaaa agaccggatt ctcgtagatc cgttttgcgg
     1621 aagcggtacg tttccgatcg aggcggcgat gatcgcagcc aacattgctc cgggaatgaa
     1681 ccgatcgttt acggctgaga catggacaaa tatcattgcg aaaaagtcat ggtatgaagc
     1741 aatcgatgag gcgacggata tgatagacga cgatatagag actgatatcc aaggatatga
     1801 cgcagatgca tctgtccttc gtgcggctcg cagaaatgca gaggaagcag gggttggtca
     1861 tctgattcat tttcagcaaa gggaagtaaa ggatctcagt catccgaaaa agtatggatt
     1921 tattattacg aatccgccgt acggcgaacg tcttgaggaa aaagaagctc ttccgaaatt
     1981 atataaagaa ttcggagacg cctttaaaaa tctggatagt tggtcggcgt atatgattac
     2041 aagctatgag gaagcggaac ggtatttcgg acggaaggca gataaaaacc ggaaaatcta
     2101 caacgggatg ctgcggactt atttctatca atttcaaggt ccaagaccgc cgcggaaaac
     2161 ggtaagagag caggggatgg cattatgaaa agaagaattg tatttgcaac gggaaatgaa
     2221 aataaaatga aagagatacg aatgatcctt tcagatctga atacggagat cgtttcgatg
     2281 aaagaagcgg gagtggatat cgatattgtg gaagacggaa tgtcttttga agaaaatgca
     2341 gagattaagg cgagatcggt agcgagaatt cttacaaacg atattgttct tgcggatgac
     2401 tccgggctgg aaatcgatta tctcgataaa gctccgggaa tttattctgc aagatttgca
     2461 ggggaagaca catcctatga tattaaaaac cgtatttttc tggatcgtct ggaaggggtt
     2521 ccggatgaag agcgtactgc gagattcgta tgtgcggtag cggctgtatt tcctgacgga
     2581 agcgtagacg tagtaaggga gacgatcgag ggaaggattg cgcatgagtc ggcgggaacg
     2641 cacggctttg gttatgatcc gatcttttat cttcctgaat atggctgtac aacggcgcag
     2701 cttagcccgg aacaaaaaaa tgaagtaagt caccgaggca aagcgctctg tgcgataaaa
     2761 cgcaaattaa aagggaaaat cggagatgat ttattatgaa agtactgata gtcagcgata
     2821 cacacggcag acatacagga ttagaccgtg ttcttgaaaa agaaggaaag atcgatcttt
     2881 ttattcattt aggtgatata gaaggcggag aaagctatat taattcggtt gtggaatgtg
     2941 agaagcatat ggtaagagga aacaatgatt ttttctcgga tttgccgcgg gaagaggaaa
     3001 tcgatatagg tgggtataaa gcctttatta ctcacggaca tccgtattat gtgtctttgg
     3061 attcagagta tataagagag gaaggcgcag ctcgaaaagt cgatatcgtg atgtttggtc
     3121 atacacataa gccgtatttt gaacagaagg atggaatcac tgtgctgaat ccgggcagtt
     3181 tggcatatcc gaggcaggaa ggcagaaagg gaagctatat gattatggag attgatcagg
     3241 agggaaaggc tcatttcaat cagaaatact tggattgacg gaatgtccgg cagagtaaaa
     3301 gacgggaaat tgcgtgtaaa aacaggatga aataaaaagt cattaaaaaa ataaaaaaat
     3361 gcaaaaaaaa tattgacaat ctaaaaacct tataatataa taggcattgc gctacgggag
     3421 agacaggaac gtaggaaatg ccggggtgtg gctcagcttg gctagagcgc ctggtttggg
     3481 accaggaggt cgcaggttcg aatcctgtca ccccgataaa ctctgcgggt gtagttcaat
     3541 ggtagaacac cagccttcca agctggatac gtgggttcga ttcccatcac ccgcttttcc
     3601 gtagaaatac ggagagcctt ttgaaaaggg gatattaaaa tgagtctgta gctcagctgg
     3661 atagagcaac ggccttctaa gccgtaggtc gagggttcga atccccccag gctcgttatg
     3721 gtgggtatgg tgcagttggt tagcgcgcca gattgtggtt ctggatatcg tgggttcgag
     3781 tcccactacc cacccttttc ttttataatt caaactggca tgcagtgtca gaaataaagt
     3841 ctttaatggg ctatcgccaa gcggtaaggc acaggacttt gactcctgca ttcgctggtt
     3901 cgaatccagc tagcccagtt atctcgggaa gctgagaaga aatagtgagg gcgtgtagct
     3961 cagttggtag agcacttgac ttttaatcaa gttgtccggg gttcgaatcc ccgcacgctc
     4021 actgtgaaac aagcgaatat gcttgtttta ttttttgtat aggcgacgtg ctaaagtccg
     4081 gggatgcccg gaaatttgga ggttgcttac aaagtcccga gatatgcctt ttggcgtttt
     4141 cggtgatcga aatataacat gcggatgtgg cggaactggc agacgcgcta gacttaggat
     4201 ctagtgggag accgtgcagg ttcgattcct gtcatccgca gtacaaaagc ctggttttac
     4261 tgggcttttt tggaggagat ggctgcaccc catctgctcc gggcaagata aataatctag
     4321 ttacaaagag tagtcgttat ctttaccaga gacagggcgg ctacttttta tttttcatat
     4381 taaggatgga gacaatcaag ccataaatta ccgttaaaga gtgtatcttg caatcatatc
     4441 aaccgttaac gatagaggaa ggcggttttg gaggcacttg gagttgagtg tactctggag
     4501 tcggctttaa atatggcgat gattaaggag ggacttgctc agaagactgc atttgaggaa
     4561 gcttattacg gactgaatgg tacggtgttt gtcgatatag aaaatgctaa cattagaacg
     4621 gcatttcata aagaacagat tgatgaatat gtgaaaaaaa tgagggaggt gttaaagaat
     4681 catgaaaaag gttgattgtc cattgatgga cgaatgtgtt gatgatgcaa tatgcttcga
     4741 tatccacatg gttgttgaag ggtgtgcacc taaattcaca gcgccgaaaa aagcagttca
     4801 gacaccggat tttaggaaaa tatgtatgaa ttgtccgaat cacagagacg attaaaagcc
     4861 acaggtcgaa aaggtctgtg gtatttttat acccattttt aagaaaggag gggaaagtgt
     4921 atgaagatac ctgaaaaaat aaaagtactt tataaggagt atatcgtgga agaaaccgca
     4981 aacctacacg atgaagccgg agatctgtac ggacagattc aatttatagc attgggctga
     5041 aagaaaatca tccgcactac aaaagccgta acctttgagt aatcagaggt cacggctttt
     5101 ttgttacggt aatgagccaa aatccggatt tatccgagat cttgacggat aaaatgtttt
     5161 atccgagttt tcgttttttg tgcattatat aaagcaatcc tatgatgctg cacattgtgg
     5221 caagaggaag caaaagaggt gtgctgtctc ctgtttttac actggtatcc gcatgtgaag
     5281 tgtttgtatt tccctgattt ccgctattgg gattttgtgg gttttgcggc tcgagtggtt
     5341 cttgtgggtc tttatttccg ccctcatcag gatcatcttt gtatgcgaat tcaaatgtat
     5401 ttaattgatt cagtatatcg gatacttccg caggcgttgc gtttggatcg gttaagatcg
     5461 catttgcaag ctctcgaagt ctggtgaaat cattccagga agcatctgtt gcgttgcctt
     5521 gagtcatatc ttttattttg ttatattttt cctgaagcaa agttttatct gcgcgtagat
     5581 ccttcatacg ctgtattaaa acggcggttt tttcatcgac ttctttttgg gtatatgtgc
     5641 cgtttaataa agcttctgcc tctaaaatgc tttgtgttaa tgcttcaaaa ctttcttccg
     5701 tatatccttc ccgggaaatg gattttgcgg cttttaacgc gtcgtttaat gcggacgtat
     5761 caacttttat ctcctgaaga ctatttcttg cattccgaac ttcatttgcc ttatcattca
     5821 tttcggaagt acacgtttct tcatcctttt tgtttaggag ttctctggcg ctgttgactg
     5881 ccttttgtaa tttgttccag ctttcttctg tgtatttgtc tttattcagc ccgtcgcaga
     5941 tcgtcagttc ttcttccaat tggcttcggt ctacgatttc tttcttttct gtacggatct
     6001 tcagtgttcc gacttcgacc catcctcttt tttccggtga tccgtttgga ctttttccga
     6061 cacgttcaaa ttgaattgtg tagctgccct ccggaaagtc tttctgtatc agttcggcgc
     6121 cctctatgcg tttttcggca tatgtatcaa tttcttctcg gcagatctgt ttctcgtttt
     6181 gatctaggat cgtcacccgg ataacgccgt gatcatctcc tttgaccgtg gaaacttgta
     6241 tcgaatttcc gttgaattta taagtcagag gccccgcatc atctgcttct gttgctttga
     6301 aatctcctgc ctggatccac tttccggaac cttgcagatc tcctgccggg atggatgttt
     6361 cggaaaaacc gccttgttcg agactgttta atgctgcctg tagtttttct gtcatttgat
     6421 cgacggctgg ttgttttacg ttttcgtcat cggctgtttt cttagcgtct gagagaactt
     6481 ttttcagttg gtataaagaa tcgtttgtat agtttccgtt tagttcctgt tctgcttttg
     6541 tgatcagcga gtttaatttt gact
//
LOCUS       DBMDXF010000103         6449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3061_length_6449_cov_2.213296, whole genome shotgun sequence.
ACCESSION   DBMDXF010000103 DBMDXF010000000
VERSION     DBMDXF010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_6449_cov_2.213296"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(102..590)
                     /locus_tag="ACZPD4_05350"
     CDS             complement(102..590)
                     /locus_tag="ACZPD4_05350"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1378525.1"
                     /translation="MSGKRTDYLTWDEYFMGVAMLSGMRSKDPNTQVGCCIVSQDNKI
                     LSMGYNGFPLGCSDDEFPWVRDGEDPLETKYVYSTHSELNAILNYSGGSLAGAKLYVS
                     LFPCNECAKAIIQAGIKEVIYDCDKYADTPSVIASKRMMDAAGVRYHQYHRTNRKIEI
                     EL"
     gene            complement(634..1266)
                     /gene="upp"
                     /locus_tag="ACZPD4_05355"
     CDS             complement(634..1266)
                     /gene="upp"
                     /locus_tag="ACZPD4_05355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1378526.1"
                     /translation="MTGKVIVMDHPLITHKINYVRQENVGTKEFREVIGEIAQLMCYE
                     ATRDLKMSDVQIKTPLMEMTGKMLSGKKLAIVPILRAGIGMVDGMLSLIPAAKVGHIG
                     LYRDPETLEPVEYYCKLPADCEEREVFVVDPMLATGGSSVAAIRMLKEKGVKHIRFLC
                     ILAAPEGVKKMQEAHPDVDIYIGALDEKLNDHGYIVPGLGDAGDRIFGTK"
     gene            complement(1266..2789)
                     /locus_tag="ACZPD4_05360"
     CDS             complement(1266..2789)
                     /locus_tag="ACZPD4_05360"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1378527.1"
                     /translation="METKIEKIDKNKINEEIIEEAGMILKNGGLVAFPTETVYGLGAD
                     ALKEEAAMKTYAAKGRPSDNPLIVHIAEYEDLRKIAVNIPAETDALAAHFWPGPLTMI
                     FEKSEVVPYGTTGGLDTVAVRMPTDEVARALIRAAGGFVSAPSANTSGRPSPTLAEHV
                     AEDLGGKIDMILDGGAVEIGLESTILDMTVTPPMILRPGAITAEMFEEVIGKVDVDET
                     LLVAESEKAPKAPGMKYRHYAPKAKLMIVEGDLREEIFAIRQLAYKAYKEGRKVGVIA
                     TTETLPFYKYGVVKNIGTRENEKTIARNLYRVLREFDEEDVEEIYSESFAAQGIGKAI
                     MNRLEKAAGHTRLSAAEIAKRQKYRRIIFISGTDSARAPMAAEMLRHEDLAQEYVIDS
                     RGLVVLFPEPANQKAEAIMKSAQMTLENHVSRQLEPETLVEDTLVLTIEEAEKNKILS
                     EYGTKANVYTLNEFVGSDGEIPNPYGKPLTAYGECYEILRELIKELAEKLNYIAEEE"
     gene            complement(2810..3571)
                     /locus_tag="ACZPD4_05365"
     CDS             complement(2810..3571)
                     /locus_tag="ACZPD4_05365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1378528.1"
                     /translation="MRKKIVFGMAIISLVAIIVLCQKAGVRLKETFRESEETNSGAVI
                     GMKEKENEKSDKKERGEKQGEAHKKRTVVVDAGHGGPDGGKTGVNGKLEKELNLIIAE
                     KVKKLLEEEGIAVIMTREEDGWLAETRIGDLKERVRIMNESKADLAVSIHQNSYHEES
                     VFGAQVFYYTTSGEGKAAAEILQNELLEIDPENKKREKANNTYYILKKTEVPTVIVEC
                     GFLSNHKEAEKLSDEAYQGKIAEAVVKGIKSFFLK"
     gene            complement(3660..5618)
                     /locus_tag="ACZPD4_05370"
     CDS             complement(3660..5618)
                     /locus_tag="ACZPD4_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR1378529.1"
                     /translation="MDEREKRDVREWSQTSDTRRRRPENNARKPVNRSGQSRQRMSEA
                     GPGRNRRPARQSSRARRRRRNAVIRWFLFFVLIIIAIGGFVIWKRYGSTNEKADLKQY
                     YGVESETDPALVIDDQVVKQSQNAEEGNTPDPVKVYDGQYYVEYSVAHNRINKRFYWD
                     SNENVLLYTLPGGSVSAAVGSKEYTDITEKKSEDYVILKTEGKIAYIALPFIQQYTNM
                     EYEVYDDPTRVVITTEWGEKKVASIKRDTQVRYQGGVKSPVLTEVKKSDKVTVLEDEN
                     DWMKVATKDGFIGYVKTNALNSVEKELVSRDYEEPEYTNISENYTINMAWHNVSNADA
                     NSYILETIASTKGLNTIAPTWFSLADTEGNITSLADADYVNYAHQSNLEVWAVLRDFH
                     GGINSYEETYQVLSYTSKRAKLINQVISKALETDVDGINLDFELVSDECGPHYVQFVR
                     ELSVKCRQNGLVFSVDNYVPQPYNAHYDIEEQAAVADYVVVMGYDEHTEGSYEAGSVA
                     SIGYLKNGISEALKSVPADKLIAGVPFYTRLWFERPKTDEEFAADEGTEAENYPNKVK
                     SSAYGMDDAAELISSMGAQTEWDDKTKQNYAQWEGDGGVYKIWLEDTQSLEEKLKVIK
                     ENQLAGVAEWSLGMEGSGVWDLILQYVN"
     gene            complement(5745..5984)
                     /locus_tag="ACZPD4_05375"
     CDS             complement(5745..5984)
                     /locus_tag="ACZPD4_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378530.1"
                     /translation="MKMDETEYTELYMELEKYEKRGIDMLLDGYQASPLQIVTAHMVK
                     EAGTYMRDYTMSGDGNIETLRFTDINEHFRAEITP"
     gene            6357..>6449
                     /locus_tag="ACZPD4_05380"
     CDS             6357..>6449
                     /locus_tag="ACZPD4_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIR1378531.1"
                     /translation="MKIEKLNENQIRCTLTHADLAARHLKLSELA"
BASE COUNT         1550 a         1761 c         1020 g         2118 t
ORIGIN      
        1 cagaatacac ggctgattct ttttttgtag aaagcggata tttgcctcta tgcacatctt
       61 ttttattgtt tccgtaatta tctctgtctg tactgtccgt atcataattc aatctcaatc
      121 tttctgtttg tacgatgata ttgatggtat ctgactcccg ccgcatccat cattcttttc
      181 gacgcaataa cgctcggagt atcggcatat ttatcacaat catagattac ttctttaatc
      241 cccgcctgta tgatcgcttt tgcacattca ttgcagggaa agagcgaaac atagagcttc
      301 gctccggcca aacttcctcc gctgtaattt aaaattgcat tcaactcgct atgtgtcgaa
      361 tatacatatt ttgtctcaag cgggtcttcg ccgtctctta cccacggaaa ttcatcatcc
      421 gaacacccta acggaaatcc attatatccc atagagagga ttttattatc ctgactgaca
      481 atacaacagc caacctgcgt attcgggtct tttgatcgca tccccgaaag catggctact
      541 cccataaaat actcatccca cgtcaaataa tctgttctct tccccgacat caactccgct
      601 cctttcatct ctgttctgtt ctctgtaagc tttttatttt gttccgaaaa tacgatctcc
      661 ggcatcgcca agtcccggaa caatatagcc atgatcattg agcttctcat caagcgcccc
      721 tatataaatg tctacatccg gatgcgcttc ctgcatcttc ttaactcctt ccggcgctgc
      781 aaggatacaa aggaaacgga tatgttttac gcctttttct ttcagcattc tgatcgccgc
      841 cacactggaa cctcccgtag ccagcatagg atcaactaca aacacttctc tctcttcaca
      901 atctgccgga agtttacaat aatattctac cggttctaat gtctccggat ctctgtaaag
      961 accgatatgt ccgaccttcg ccgccggaat cagagataac attccgtcta ccattccgat
     1021 tcccgcacga aggatcggta cgatagccag ttttttgcct gaaagcattt tccctgtcat
     1081 ctccataaga ggtgttttga tctgaacatc actcattttc agatctctcg tcgcctcgta
     1141 gcacatcaac tgtgcaattt ccccgatcac ctcacgaaat tcttttgttc ctacgttttc
     1201 ctgtcttaca taattgatct tatgtgtgat caaggggtga tccatcacaa ttacttttcc
     1261 tgtcattatt cttcctccgc aatgtaattt aatttttctg caagttcttt tattaattca
     1321 cgcaatatct catagcattc tccgtaagct gtcaaaggtt tcccatatgg attcgggatt
     1381 tctccgtcgc ttcccacgaa ttcattcaac gtataaacgt tagcttttgt tccgtattcg
     1441 gacaagattt tgtttttctc ggcttcctcg atcgtaagga caagagtatc ctccaccagc
     1501 gtttccggtt caagctgtct ggaaacatga ttttcaaggg tcatctgggc gctcttcatg
     1561 atcgcctccg ctttctgatt cgccggttcg ggaaacagca cgaccaatcc ccgtgaatca
     1621 atcacatatt cctgcgccag atcttcatgc cttaacatct ccgccgccat cggcgcccgg
     1681 gcagaatccg taccgcttat aaatatgatt cttcggtact tctgccgttt tgcgatctcc
     1741 gccgccgaaa gacgcgtatg ccccgccgct ttttccaaac gattcataat cgcttttccg
     1801 attccttgcg cggcaaaaga ttcactgtaa atctcttcca catcttcctc atcaaattct
     1861 ctgagcactc tatagagatt tcgagcgatc gttttctcat tttcccgtgt accaatattt
     1921 ttcactacgc cgtatttata aaaaggaagt gtctctgtcg tagcaataac ccccactttt
     1981 cttccttctt tataagcctt gtatgcaagc tggcgaatcg caaaaatctc ctcccgcaga
     2041 tctccttcta caatcatcag ttttgctttc ggagcgtaat gtctgtactt catccccgga
     2101 gctttcggag ctttctcact ctccgccaca agaagcgtct catcaacatc tacttttccg
     2161 atcacttctt caaacatttc cgcagtaatc gctcccggtc tcaagatcat aggcggcgtc
     2221 accgtcatat caagtatcgt agactctagt ccgatctcaa ctgctcctcc gtctaagatc
     2281 atatcgatct ttccgcccaa atcttccgca acatgttccg ccaacgtcgg acttggtctt
     2341 cccgaagtat ttgcgctcgg ggcagaaacg aatccgcccg ctgcccgtat aagcgctctc
     2401 gccacttcat ctgtaggcat acgaaccgcc actgtatcaa gacctcccgt cgtaccataa
     2461 ggaacaacct cgctcttttc aaagatcata gtaagcggtc ccggccagaa gtgagctgca
     2521 agggcatctg tttcagccgg gatattaaca gcaatcttcc tcaaatcttc atattctgca
     2581 atatgcacga tcagaggatt atccgacggt cgcccttttg ccgcatacgt cttcatcgcc
     2641 gcctcctctt tgagcgcatc cgctccgagc ccatataccg tctcggtcgg aaaagcaacc
     2701 aaaccgccgt ttttcagaat cattcccgct tcttctataa tctcttcatt tattttgttt
     2761 ttatcgatct tttctatttt tgtttccatg ccctatatta tagcattatt tatttcaaaa
     2821 aaaaactctt aattcctttt actacagcct ctgctatttt tccttgatac gcttcatcag
     2881 aaagtttttc cgcttctttg tgattactca aaaaaccaca ttccacaatt actgtcggca
     2941 cttctgtttt cttcaatata taataagtat tatttgcttt ttcacgcttt ttattttccg
     3001 gatcaatttc cagaagttca ttctgcaaaa tttccgccgc cgctttccct tccccggacg
     3061 tcgtataata aaacacctgc gctccaaaaa cactttcttc atgataactg ttttgatgta
     3121 tacttacagc aagatctgcc tttgattcat tcataatgcg aacccgctct ttcaaatctc
     3181 cgatcctggt ttccgcaagc catccgtcct cttcacgtgt catgatcact gcaatccctt
     3241 cctcttcaag aagctttttc actttctctg cgattattaa gttcagctct ttctcaagct
     3301 tcccgttgac tcccgttttt ccgccgtcag gaccgccgtg tccggcatcc actacaaccg
     3361 ttctcttctt atgcgcttcc ccttgttttt ccccgcgctc ctttttatcc gatttctcat
     3421 tctccttttc tttcatccct ataaccgccc ccgaattagt ttcttcactt tccctaaacg
     3481 tctccttaag cctcacccct gctttttgac acaaaacaat gattgccaca agagaaataa
     3541 tcgccattcc gaaaactatt tttttccgca tataaaaaca ccctcctttt ccatataaat
     3601 atatatgcaa aaagaggatg tccttttcag atattttatt tcatgtttta gttcacattt
     3661 taatttacat attgcaggat gagatcccac acgccggaac cttccattcc aagactccac
     3721 tccgcaacac ccgcaagctg gttttcttta ataactttca gcttctcttc cagcgactga
     3781 gtatcttcta accaaatttt ataaacgcct ccgtcgcctt cccactgggc ataattctgc
     3841 ttcgtcttat catcccattc cgtctgtgct cccattgatg agataagttc tgccgcatca
     3901 tccattccat aagcggaact ctttacttta ttcggataat tttcggcttc cgttccctca
     3961 tctgccgcaa attcttcgtc agtctttggc cgttcaaacc aaagccgggt ataaaacggt
     4021 actcctgcga tcaatttatc cgccggtact gacttaaggg cctctgatat tccgtttttc
     4081 agatacccga tcgacgcaac cgaacctgct tcatacgatc cttctgtatg ttcatcatat
     4141 cccataacaa caacatagtc cgccactgcc gcctgttctt caatgtcata atgtgcatta
     4201 taaggctgcg gaacataatt atctacagaa aaaacaagtc cattctgacg gcacttcacc
     4261 gaaagttcac gcacgaactg cacataatgc ggtccgcact catcggatac aagttcaaaa
     4321 tcaaggttaa ttccatccac gtcagtctcc agcgcttttg atataacttg attgatcagt
     4381 tttgcccgct tcgacgtata gctcagcact tgatatgttt cttcataaga attgattcct
     4441 ccatgaaaat ccctcagcac tgcccatact tccaaattag actgatgtgc gtaatttaca
     4501 taatccgcat ctgcaagcga tgtaatatta ccttctgtat ccgcaagact gaaccatgtc
     4561 ggcgcaatcg tattcagccc ttttgtcgac gcaatcgttt ccaatatata actattagca
     4621 tcggcgttag aaacattatg ccacgccata ttgatcgtat agttttccga aatattcgta
     4681 tattccggtt cttcataatc ccgggatacc agctcttttt caacactgtt gagcgcattt
     4741 gttttcacat atccgataaa cccgtctttt gtagccactt tcatccagtc attctcatct
     4801 tcaagaacag ttaccttatc cgatttcttt acttctgtca aaaccggact tttcacaccg
     4861 ccctgatagc gtacctgcgt atcccttttg atggaagcca ctttcttctc tccccactca
     4921 gttgtaatca ctacgcgggt cggatcatca tacacttcgt attccatatt cgtgtattgc
     4981 tggataaacg gaagggcaat atatgcaatc tttccttctg ttttcaggat cacataatct
     5041 tcactctttt tctctgttat gtctgtatat tctttactgc ctaccgctgc cgaaacactg
     5101 cctcccggaa gtgtataaag aagaacgttc tcattagaat cccaataaaa ccgcttattg
     5161 atccgattat gagcaacaga atattcgaca taatactgcc cgtcataaac tttcaccgga
     5221 tccggcgtat ttccttcttc tgcattctga gactgtttca caacctgatc gtcaattaca
     5281 agcgcaggat ctgtttctga ctccactcca taatattgtt tcaaatcggc cttctcattc
     5341 gttgaaccgt atcttttcca aataacaaat ccgcctattg caattatgat caacacaaaa
     5401 aacagaaacc accgtataac ggcattccgc cttcttctcc ttgctctgct gctttgtctc
     5461 gcaggtcttc tattccgtcc aggtcccgct tccgacattc tttgtctgct ttgtcccgaa
     5521 cggtttaccg gtttccgggc attgttttca gggcgtcgtc tgcgggtatc tgatgtctga
     5581 ctccattccc gcacatcccg tttttctctt tcgtccatgc cgtacctcct actagcaatt
     5641 atacgcgtac attatatcac acttttaaca cgcgtcgtta ataatttgcc tctttcagca
     5701 gattttagac atcttttttc acacaacctc cactttgtcc ggcattaagg ggtaatttct
     5761 gcccgaaagt gttcattaat atcagtaaat ctaagagttt ctatattgcc atccccactc
     5821 atcgtataat cgcgcatata tgttcccgct tcttttacca tatgtgccgt cacgatctga
     5881 agcggacttg cctgatatcc gtcaagcagc atatcaatcc cacgcttctc atacttttcc
     5941 agctccatat ataattctgt atattccgtt tcatccattt tcatctcccg caatactcct
     6001 ttccatgcaa accgtgataa atatagtttt cctgcccttt cattatgtat cgtacggagg
     6061 tttgtgacat acatccggat tttcaggcag ggcaaaagac agccgatacg gaaataagag
     6121 tttaaacagc attccctcct ttcattttat ttacttatca gtgaatacta tcttttgtaa
     6181 gtgccattat attgtccgca ttttatgttt tcaatgtgtt ttttgttcat aaccgcatgg
     6241 gatttgttga taagtcttta catccttttc ttttttgttt atacttatct tgaggatatg
     6301 tatctatttt tattcctgat gcatattatc ttatatagta taaaggatgt gattgtatga
     6361 agattgaaaa acttaatgaa aatcagattc gttgtacatt gacacatgct gatctggcag
     6421 cgcgccatct gaagctaagc gagcttgct
//
LOCUS       DBMDXF010000104         6442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3068_length_6442_cov_2.109131, whole genome shotgun sequence.
ACCESSION   DBMDXF010000104 DBMDXF010000000
VERSION     DBMDXF010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3068_length_6442_cov_2.109131"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="ACZPD4_05385"
     CDS             complement(<1..411)
                     /locus_tag="ACZPD4_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HIR1378532.1"
                     /translation="MRDGMITEEQCGENFAYILEDKTDFLITEYKMMHGQEADFLLKC
                     VKMLYNGKTELYYDTKSCLPLAIQSGAEDTEGMLSVLGNILHEVRRVTENGFLSLLKL
                     DISADKIWVDPATRKIRFVYLPVAERLHKDVVEFE"
     gene            complement(451..2037)
                     /locus_tag="ACZPD4_05390"
     CDS             complement(451..2037)
                     /locus_tag="ACZPD4_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR1378533.1"
                     /translation="MGEKGKKKSRILLIVCGLLLLAGAIIGIVMFRMQAGASAYNESI
                     KTAEKYADSGEYELAVLTYENAIKDRPKEEEGYLGLADVYLKRGESSAAKVLLQRGYM
                     HTNSSKIQYMLSGIKDGSLLAEFDDSETKKETMQSFGVFSFNTAFLQKLENYDYTDYC
                     DQFGSYPQIVKTGKGEVEVVHKDLAGTCCYSNTKEHDDIVNVKTDKPAKDGMPEIVRL
                     DSISQLFRNFPGKASLAELNAISSSKVAPITDEERVYVELSTGSLLIRVETDASGNII
                     SDKAWNEIILKEANENRSENGTLNGVVIDAMTGEGVPAAKLEFKAKENAENSTHVTSG
                     KDGSFSIELAADVYEVTISADGYVDETFEFEMEKDKNYSGEQFTISPELAAGSARIVL
                     EWNAQPQDLDSYLWGNTDKGDDLYVNFRKRTCEGRDGLLAELDVDDTNGYGPETITLN
                     DLNGVYTYSVVDYRTTGTLQQYGATVKVYLPGKSAPTVITLDPNAGVENVWEVFELDH
                     GELKILNRAPAEENLRPGSK"
     gene            complement(2144..2341)
                     /locus_tag="ACZPD4_05395"
     CDS             complement(2144..2341)
                     /locus_tag="ACZPD4_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378534.1"
                     /translation="MKIENKRQNQDAGKKRVRNGGMKMSGIMICVTGVVVIVAASLAA
                     GSVLYLLQRKKKKLKSEQINL"
     assembly_gap    2417..2426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2427..4244)
                     /locus_tag="ACZPD4_05400"
     CDS             complement(<2427..4244)
                     /locus_tag="ACZPD4_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1378535.1"
                     /translation="MLQSGDIISGMYQVIREIGTGGMGVIYIGYHLHLQKQIVIKKIK
                     ETCVDRVNVRGEADILKQLRHTYLPQVYDFLEVGNDVYTVMDYIPGENLQYYIDHNYT
                     FPEETILLWMRELCEVLDYLHTRKPRILHSDIKPANIMVTPEGDICLIDFNISLDGSV
                     TQEVQGLSRRYASPEQFRCAMDKMYGRKSSETLDERMDIYSLGAVFYRMMSGYLPDAQ
                     NGVPYALSQIDIPYADGLKRIVDKAMERDVSRRFRTAKQMEEALLHMEKYDPKYRRLT
                     NFQILTATAWGFCILAGIFLIASGVRQRGKELWQQEYQEFYAVVGSGDSENIIREGTE
                     LLNNSSLQGYMKRNKEQKAEVLHAVGDGYFYSEQYDTAGKYYEEALSCDSGNSLYVRD
                     YMTAMARNGSPVDIWTIQSEYPEAAIDEAATVFVQAQSMYAKGDQKSAVSLCEEALGK
                     SMDAELNAQIYLLEAELYLEQGDINSAADAAAQAVKLDESSNVLRKAAVTAYQAGNEE
                     RIGTVKNQWYRQALAYYEMLCQKNDPSYEDRLGRALLLRVLGKYRDSTDVLEEMKIDY
                     PKDYKVQMWICYNYLDEASEEGTYSNVSGDLKFTYHSCRN"
     gene            complement(4358..4816)
                     /locus_tag="ACZPD4_05405"
     CDS             complement(4358..4816)
                     /locus_tag="ACZPD4_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378536.1"
                     /translation="MNNKVKECNIDFVIDKTPPTVVITGIEESSYRADEREMTINLSD
                     NTAVKSVDVIIDGRSVATYAQKEIEKAGGKISYMIEGASEPQKIEAAALDMAGNETTS
                     EMHKVLVTSSVWIQYINNTPLLIGSILGIVLVAGGLIWFFVIRKKKEESK"
     gene            complement(4813..>6442)
                     /locus_tag="ACZPD4_05410"
     CDS             complement(4813..>6442)
                     /locus_tag="ACZPD4_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1378537.1"
                     /translation="FAVVADQTKPEIEITNLSTPRNGIFKGDVKLHIEVTDPENNKTY
                     SGLEEVWYDVKASGNVVKEVRYPSLVDNSANPVKGNQSWSGDITIPAAEYNSNDVKVQ
                     VHARDFSGNVYDSEIVPLKIDITHPTIAVTYDLNTPLNERYYKDVRTATVVVIERNFD
                     ESAVRFNITNTDGTQPAISGWTHSANAGVSDDATHTCQVTFAADGDYTFTLETTDLAG
                     NASSYNRVDDFTIDRTVPTIQVSYDNNSAATPGYFNANRTATVTVNEHNFNAAEVNAQ
                     ITAALQGSGVAAPGLGGWSTNGDVHTASVTFSADADYTFDVDYTDLAGNAAADYTQDK
                     FTVDKTDPEVEFFDIEDKSANNGTVAPGVKYSDVNYMESGVDITIKGAKHDKTELSGN
                     RTNIANGESIKMEDFKHDEETDDVYTMTAVIKDKAGNETEKEVMFSVNRFGSNYIFSE
                     TTEKFLDDVYANKPKDLVVTEVNVDSLVFNGISYGLDGTKKELKPGSDYTVKQNAVAK
                     VHGKNIHTRSKKKTLKKKVATVLRSTRKTRRQIR"
BASE COUNT         1426 a         1729 c         1135 g         2142 t
ORIGIN      
        1 ctcaaattcc actacatctt tatgaagtct ctccgccaca ggaagatata cgaacctgat
       61 ttttcgcgtt gcaggatcga cccatatctt atctgccgaa atatccagtt tcaaaagcga
      121 aagaaatccg ttttctgtca cccttctgac ttcatgcaga atatttccca aaacagaaag
      181 catcccttct gtatcttccg cccctgactg gatcgcaagc ggcagacagg atttcgtatc
      241 gtaatacagt tctgtcttgc cgttatacag catcttcaca cacttcaata gaaaatctgc
      301 ttcttgaccg tgcatcattt tatattctgt tatcaaaaaa tctgtcttat cttctaaaat
      361 ataagcaaag ttctctccac actgttcttc cgtaatcatt ccatctctca ttttcctgct
      421 ctcatcttcc tgctctcatc ttcgtctggt ttattttgaa cccggacgca aattttcctc
      481 cgccggagcg cggttcagta ttttcagttc tccgtggtca agctcaaata cttcccatac
      541 attttcaacg cctgcattcg ggtcaagtgt gatcactgtc ggagcgctct tgcccggaag
      601 atatactttc accgttgcgc cgtactgctg caaagttccc gtcgttctgt agtcaacgac
      661 agagtatgta taaacgccgt tcaggtcgtt cagcgtgatc gtctccggtc cgtatccgtt
      721 tgtgtcatcc acatcaagtt ctgccagaag cccgtctctt ccttcgcatg tccgtttcct
      781 gaagttcacg tacaagtcat ctcctttatc cgtatttccc cacagatacg agtcgagatc
      841 ctgaggctgt gcgttccatt ccaatacgat acgcgcgctg cccgccgcca gctccggtga
      901 aatcgtaaac tgctctccgc tgtaattttt atctttctcc atctcaaact cgaacgtttc
      961 atccacatac ccgtccgcgc tgatcgtcac ttcgtacacg tctgccgcca gttcgatcga
     1021 gaacgatccg tcttttcccg acgtaacatg agtagaattt tcagcgtttt ctttcgcttt
     1081 aaattcaagc ttcgccgccg gaacgccttc tcctgtcata gcatcgatca caacgccgtt
     1141 cagcgttccg ttttccgagc ggttttcgtt tgcctctttc aaaataattt cattccacgc
     1201 tttatcggaa ataatgtttc ccgacgcatc cgtctcaaca cggatcagga ggcttcctgt
     1261 agagagttcc acataaactc gttcctcgtc tgtgatcggc gctactttgg aactggagat
     1321 tgcgttcagt tctgcaaggc ttgcctttcc cgggaaattt ctgaacaact gcgaaatcga
     1381 atctaaacgc acgatttccg gcattccgtc cttcgccggt ttgtctgtct ttacatttac
     1441 aatatcgtca tgctctttcg tgtttgaata acagcatgtt cccgccagat ctttatgtac
     1501 gacttctact tctccttttc cggtctttac gatctgcgga tatgagccga actgatcgca
     1561 gtagtccgta tagtcataat tttccagctt ctgaagaaat gcagtattaa aagaaaaaac
     1621 gccaaatgac tgcattgtct ctttctttgt ttctgaatcg tcaaattccg ccaaaagcga
     1681 accgtcctta atcccgctta acatatactg gatctttgaa gaattcgtat gcatgtagcc
     1741 tcgctgaagc aaaacttttg cagcggagct ttcccctctc ttcaaataca catctgccag
     1801 tccgagatat ccttcttctt ctttcggtcg gtctttgatc gcattttcat atgtaagcac
     1861 cgccagttca tactctccgc tgtccgcata tttttccgct gtttttattg attcgttata
     1921 cgcgctcgct cctgcctgca tccgaaacat cacgatcccg ataatcgccc ctgccaaaag
     1981 aagcagaccg cagacgatca gcagaatccg gcttttcttt tttccttttt ctcccactgc
     2041 tccttctcct ctctttcttt ttttactgag acgtttcgta aacgccccta ttttattttt
     2101 cacatccgtt tatctagcat ttactcgtta tcttttccgc tatttacaag ttgatttgtt
     2161 cactttttag tttcttcttt tttctttgaa gcaaatataa aacgcttcct gctgccaacg
     2221 acgcggccac gatcactaca acgcctgtca cacatatcat gatcccgctc atcttcattc
     2281 ccccatttcg cactctcttt ttacctgcat cctgattctg tcttttattt tctatcttca
     2341 atcttcgtgc tgcttcacat ctcgcttccg gttattcaag ttctttcatt gtctcttcca
     2401 gcatttccat atcaggnnnn nnnnnnattt ctgcaagaat gataagtaaa tttcagatcg
     2461 cctgacacat ttgaatacgt tccttcttcc gatgcctcgt ccaaatagtt gtaacagatc
     2521 cacatctgaa ctttatagtc tttcggataa tctattttca tctcttccag cacatccgta
     2581 ctgtctctat attttccgag aactcgaaga agaagcgccc ttcctaaacg atcctcatat
     2641 gacggatcgt ttttttgaca gagcatctca taatatgcaa gagcttgcct gtaccactga
     2701 tttttgaccg ttcctatcct ctcttcgttt cctgcctgat atgccgtcac tgccgctttt
     2761 ctaagaacat tgctgctttc atctagtttc actgcttgtg ccgccgcatc tgccgcagag
     2821 tttatatccc cctgctcaag atataattct gcttccagca gataaatttg tgcattcaac
     2881 tctgcatcca tactttttcc aagcgcttct tcgcataaag aaaccgcgct cttttgatcg
     2941 ccttttgcat acattgactg cgcctgaaca aatacagtag ctgcctcatc gatcgccgct
     3001 tccggatatt cactttgtat cgtccatatg tcaaccggac ttccgtttct tgccattgca
     3061 gtcatataat ctctcacata taaagaattc ccgctgtcgc acgacaaagc ttcctcataa
     3121 tactttcccg ccgtgtcgta ttgttcacta tagaaatatc cgtcccctac agcgtgaagt
     3181 acctccgcct tctgctcctt attccttttc atataacctt gcaaagaaga attgttcaga
     3241 agttctgtgc cttcccttat tatattttcg gaatcccctg atcccacaac ggcgtaaaac
     3301 tcttgatatt cctgctgcca cagctctttc ccccgctgac gcactcctga cgcaataaga
     3361 aagattcctg ccagaataca aaatccccat gcagttgcgg tcaatatctg aaagttcgtc
     3421 agacggcggt acttcggatc gtatttttcc atatgcaaaa gagcttcttc catctgtttt
     3481 gccgtccgaa atcttcttga aacgtcccgt tccatcgctt tatcgacgat ccgttttaaa
     3541 ccgtccgcat acggaatatc aatctgtgac agcgcatacg gaacaccgtt ttgagcatca
     3601 ggcaaatacc cgctcatcat ccggtaaaag accgctccca gactgtaaat gtccattctc
     3661 tcatccagtg tttcactgct tttcctgccg tacattttgt ccatcgcaca tcggaattgt
     3721 tccggagatg cataccgtct gctcagtccc tgaacttcct gcgttaccga tccgtccagt
     3781 gagatattaa aatcaataag gcaaatgtct ccttccggcg tcaccataat atttgccggc
     3841 ttaatgtcgc tgtgaaggat tcgtggcttt ctcgtatgaa gataatccaa aacctcacat
     3901 aactcacgca tccacaacag aatcgtctct tccggaaatg tataattatg gtcgatataa
     3961 tactgcagat tttcccctgg tatataatcc atcaccgtat atacgtcgtt tcctacttcc
     4021 aagaaatcgt aaacctgcgg cagatatgta tgtctaagct gctttaagat atcggcttcg
     4081 cctcttacat ttacacggtc cacgcacgtt tcttttattt tctttattac gatctgtttt
     4141 tgaagatgaa gatgataccc gatatagatc actcccatgc cgcctgttcc gatctctcgt
     4201 atcacctgat acattccgct tataatatct ccgctttgca acataatttc tttcctcata
     4261 aagacgaaag gcggctgtcc gaatccgttt gttcagacag ccgcctcctt ttcagctttt
     4321 tcttatttct gccgtatttt tatcagcttc agacttttta tttgctttct tctttctttt
     4381 ttctgatcac aaagaaccag atcagtccgc ctgctacgag aacgattccg aggatcgatc
     4441 cgataagaag cggtgtattg ttgatatact gaatccatac acttgatgta acaagcactt
     4501 tgtgcatttc cgaagtcgtt tcatttcctg ccatatcaag cgctgctgct tcgatcttct
     4561 gcggttctga tgcaccttcg atcatgtaac tgatctttcc gcctgccttt tcaatctctt
     4621 tctgtgcata agttgcaact gatcttccgt cgatgattac atccacgctc ttaactgccg
     4681 tattgtctga caggttgatc gtcatttctc tctcgtctgc acgatagctt gactcctcga
     4741 tacctgtgat aacaactgtc ggaggcgtct tatcgattac gaaatcgata ttgcactctt
     4801 ttactttgtt gttcatctta tttgtcgcct tgtcttccga gtcgatcgta acactgtagc
     4861 gaccttcttt ttcaaagttt tcttttttga tcgtgtatgt atattctttc catgaacctt
     4921 cgccaccgca ttttgtttta ctgtataatc gctgcccggc ttcagttctt tcttcgttcc
     4981 gtccaatccg taagagatac cgttgaacac aagtgaatct acgttcacct ctgtaacaac
     5041 aaggtctttc ggtttgttcg catatacatc atcaaggaac ttctctgttg tctcagagaa
     5101 aatatagttt gatccgaaac ggttgactga gaacataacc tctttttccg tttcatttcc
     5161 ggctttgtct ttaataaccg ccgtcatcgt gtaaacgtca tccgtctctt catcatgttt
     5221 gaaatcttcc atcttgatgc tctcgccgtt tgcgatattt gtacggtttc cgcttaattc
     5281 tgtcttgtca tgtttcgcgc ctttgatcgt aatatcaacg ccgctttcca tgtagtttac
     5341 atcactgtat ttcacgccag gagcaactgt tccgttattt gccgatttat cttcgatatc
     5401 gaagaactca acttcaggat ctgtcttatc taccgtaaat ttatcctgtg tgtaatctgc
     5461 cgctgcattt cctgcaaggt ctgtgtaatc aacatcaaat gtataatctg cgtctgcaga
     5521 gaatgttacg cttgctgtat gcacgtctcc gtttgtgctc catccgccaa gtcccggagc
     5581 tgccacgccg cttccctgca aagctgccgt gatctgcgcg ttgacttccg ctgcgttaaa
     5641 gttatgctcg ttgactgtaa ctgttgccgt tctgttcgca ttgaaatatc ccggcgtagc
     5701 cgcgctgttg ttgtcgtaag atacttggat cgtcggaact gtccggtcga tcgtaaagtc
     5761 gtctacacgg ttgtagcttg atgcatttcc tgcaaggtct gtcgtctcaa gtgtaaatgt
     5821 gtaatctccg tctgccgcaa acgttacctg acatgtatgt gtcgcatcgt cagatactcc
     5881 tgcgttcgca ctgtgtgtcc atccgctgat cgccggctgt gtgccgtctg tattcgtgat
     5941 gttgaaacga actgcggact cgtcaaagtt tctctctatc acaacaaccg ttgccgttct
     6001 tacgtctttg tagtatctct cgttgagagg tgtgttcaag tcgtacgtta cggcgattgt
     6061 cgggtgagtg atgtcgattt taagaggaac gatttcacta tcgtatacat ttcccgagaa
     6121 atcacgggca tgaacctgaa ctttcacatc attactatta tactctgctg ccggaatcgt
     6181 aatatccccg ctccaactct gattgccttt gaccggattt gcgctgttat ctacaagtga
     6241 tggataacga acttctttta ctacgtttcc tgatgccttt acgtcatacc atacttcttc
     6301 aagacctgaa tatgtcttgt tgttttcagg atctgtcact tcgatatgga gttttacatc
     6361 acctttgaat attccgtttc tcggcgtgct taagtttgta atctcaatct ccggtttcgt
     6421 ctgatctgcc acaactgcaa at
//
LOCUS       DBMDXF010000105         6416 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3078_length_6416_cov_1.643705, whole genome shotgun sequence.
ACCESSION   DBMDXF010000105 DBMDXF010000000
VERSION     DBMDXF010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_6416_cov_1.643705"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="ACZPD4_05415"
     CDS             complement(<1..434)
                     /locus_tag="ACZPD4_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1378538.1"
                     /translation="MHHVTKTFGKVVANKNVDLELKTGEILALLGENGSGKTTLMNML
                     SGIYFPDYGEIYVKGKEVTIRSPKDSFTLGIGMIHQHFKLVDVLTAAENIVLGLEGKT
                     VLKRREINHAKPMGKFLTVLGVVALIFVFGFGVYSLKLQSTP"
     gene            complement(651..1823)
                     /locus_tag="ACZPD4_05420"
     CDS             complement(651..1823)
                     /locus_tag="ACZPD4_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1378539.1"
                     /translation="MKKKMLSAFLCAAMAVTLVAGCGAPAADAGKDKGGDSKKEASEG
                     IPKDEIKVGFVHISDPSDMGYTYNHDLGTKEMQKELGLSDDQIINKYNVPEDAECDTA
                     LRELVEAGCNIIFATSFGFEDYVKEVAAEYPDIQFCHATGFQASDSGLDNFHNYFASI
                     YEGRYLAGIAAGLKTETNKLGYVAAHPFAEVISGYTAFYLGAKSVNPDVTMDIMYTNS
                     WNDPTIESQVAKALIDRGCDVISQHSDSTAPATTAEDNGVWQVGYNNDMIAAAPNASL
                     VSPRIDWGIYVTEAVQAVIDGKEIPADWCKGYADGAVYLSPLNEKIAAEGTQEAIDKA
                     AEALKNGELFVFGGPLKGVSPDGEKIEVAEGDHFPEQEEASAPSWQYIIEGCNVVQ"
     gene            complement(1945..4485)
                     /locus_tag="ACZPD4_05425"
     CDS             complement(1945..4485)
                     /locus_tag="ACZPD4_05425"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1378540.1"
                     /translation="MKQYEVTGMSCAACSARVEKAVSKIEGVTSCSVNLLTNSMGVEG
                     AVDAQTVIEAVEAAGYKAAEKESRTGQEAGRMEKSGELKDKETPEMKKRLIASLCFLI
                     PLMYLSMGHMMWGWKVPEIVSENHVAMGIIQLLLTTAVMVINQKFFISGVRGLFHKAP
                     NMDTLVALGAGASYGYSIYALFAMTDAQMRGNAAEVMTYMHEFYFESAAMILTLITVG
                     KLLEARSKGKTTDALKGLMGLAPKTATVVRGGEEVKIGIEQVQKGDLFVVRPGESIPT
                     DGIVVEGSGAVDESALTGESIPVDKKERDCVSGATINQSGFLRCKATRVGEDTTLSQI
                     IHMVGDAAATKAPIAKVADKVSGVFVPVVMAVAVLTIIVWLAFGETIGFALARGISVL
                     VISCPCALGLATPVAIMVGNGVGAKNGILFKTAVSLEETGKIDVVVLDKTGTITKGRP
                     TVTDILPGSAFSESELLTYAYILEKKSEHPLAHAILKKAEEEAIQTKEEAKNFTAVSG
                     NGLTGEIGGDKFFGGNLKFAEGRTVISEAVRKQAESFASEGKTPLFFGRNEELIGIIA
                     VADIIKEDSPQAVRELKNMGIKVVMLTGDNERTAKAIGEQAGVDEVIAGVLPDGKEDV
                     IKKLKEEGKVAMVGDGINDAPALTRADVGIAIGAGTDIAIDAADIVLMKSRLSDVPAA
                     VRMSRAALRNIHENLFWAFFYNVIGIPLAAGIWIPIFGWKLNPMFGAAAMSLSSVCVV
                     SNALRLNLFKLYDGSKDRKRSQKNKKNSEEEKENKTMKKVMKIEGMMCGHCEARVKKV
                     LEALDGVEQAEVSHETGTAVVEMTEEVADEVLKKAVEDQDYKVLGIDF"
     gene            complement(4539..4850)
                     /locus_tag="ACZPD4_05430"
     CDS             complement(4539..4850)
                     /locus_tag="ACZPD4_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1378541.1"
                     /translation="MEKEKKCPECTHRIKKRTEEETKLMINRLNRIEGQVRGIRRMVE
                     NNAYCPDILIQVSAITAALNSFNKVLLSEHIRTCVSEDIREGKDETIDELVTVLRKLM
                     K"
     gene            complement(5090..5845)
                     /gene="aroD"
                     /locus_tag="ACZPD4_05435"
     CDS             complement(5090..5845)
                     /gene="aroD"
                     /locus_tag="ACZPD4_05435"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR1378542.1"
                     /translation="MNTVNVRKVEIGKGIPKICVPVVGITRDDIIDAACKAKETADLV
                     EWRADWYEDVLDFKKTEKMMEELRETLGDIPLLFTFRTLKEGGEKEIEKSVYVKLNEM
                     AVKTGFADLVDAEAFTGTDEVNTIVETAHLYGVKVIASNHDFQKTPPKEEIVSRLCFM
                     QECGADIVKIAVMPQSKKDVLTLLLATEEMVREHAKCPVVTMSMSEVGVVSRICGEAF
                     GSALTFGAVKKASAPGQLGAEELRMVLKILHDV"
     gene            complement(5888..6235)
                     /locus_tag="ACZPD4_05440"
     CDS             complement(5888..6235)
                     /locus_tag="ACZPD4_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR1378543.1"
                     /translation="MLFVCYPKCSTCMKAKKWLEEKEIEFEQRDIKEEKPTAEELKTW
                     WRKSGLPLKRFFNTSGKLYKEMQLKDRIPNMSEDEQIELLASDGMLVKRPILVQNEKV
                     LVGFKEKEWQEIL"
BASE COUNT         1578 a         1772 c          977 g         2089 t
ORIGIN      
        1 aatggagtgg attgtagttt caaagaataa acaccgaatc cgaacacaaa tatcaatgcc
       61 acaactccca gaactgtcaa aaattttccc ataggttttg catgattgat ctcacggcgt
      121 tttaacaccg ttttcccttc caatcccaac acgatatttt ccgctgccgt caacacatcc
      181 acaagcttaa aatgctgatg gatcattccg attccgagag taaaagaatc cttcggggaa
      241 cgaatcgtta cctccttccc ttttacataa atctctccgt aatccggaaa ataaattcct
      301 gacaacatgt tcatcaatgt tgtttttcca cttccgtttt caccgagaag cgcaaggatc
      361 tcccctgttt tcagttcaag atctacattt ttattggcaa cgactttacc gaatgttttc
      421 gttacatgat gcatttttat tgcatactgc gctgcttctc ccaaattttt ttcctccttc
      481 ttctctcctg ccgtatcttt ctcatttcct catgaaatcc tcatagaaca aagcggtatt
      541 ccgcttaaaa aacatttaat gttataaaca aagccgtccg aagacggctc tgttttcatt
      601 ttccgaagat atcttctttc ggatttattt tttagaatta ctttatacaa ttactgaact
      661 acgttacatc cctcgataat atactgccat gacggtgcag aagcctcttc ctgttccggg
      721 aaatgatctc cctctgcaac ttcaatcttc tctccgtccg gacttactcc tttaagcgga
      781 ccgccgaata caaaaagttc tccgtttttc agcgcttccg ccgctttatc gatcgcctcc
      841 tgcgttccct ctgctgcgat cttctcattc aatggagaca aataaacggc tccgtcggca
      901 tatcctttgc accagtcagc cggaatttct tttccgtcga tcactgcctg aacagcttct
      961 gtcacataaa ttccccagtc aatacgagga gaaactaaag acgcattcgg tgcagcggca
     1021 atcatatcat tattatatcc aacctgccat actccattat cctcagctgt cgttgccgga
     1081 gctgtagaat ccgaatgctg ggagatcaca tcgcatccgc ggtcgataag agcttttgca
     1141 acctgagact cgattgtcgg atcattccat gaatttgtat acataatatc cattgttaca
     1201 tcaggattca cactttttgc tccaagatag aacgctgtat atccgctgat aacctctgca
     1261 aacggatgtg ccgctacgta gccgagttta tttgtttctg tcttaagtcc tgccgcaata
     1321 cctgccagat aacgtccctc ataaatagac gcaaaataat tatggaagtt atccagtcct
     1381 gaatccgatg cctggaatcc tgtcgcatga cagaactgga tgtccggata ctctgcggct
     1441 acttctttca catagtcttc aaacccgaag cttgttgcaa agataatgtt gcatcctgct
     1501 tctacgagtt ctctaagagc tgtatcacat tctgcatctt ccggcacatt atatttgttg
     1561 atgatctggt catcgctcaa tcccagttct ttctgcattt cctttgttcc cagatcatga
     1621 ttgtatgtat atcccatatc gctcggatcg gaaatatgta caaatcctac cttgatttca
     1681 tctttcggaa ttccttccga cgcctctttt ttggagtctc ctcctttgtc tttgcctgcg
     1741 tctgccgccg gagctccgca ccctgcaacg agagtgactg ccatagctgc acacaagaat
     1801 gcgctgagca ttttcttctt cattgttttt cctcctttta ttccgttctt tttaaaagaa
     1861 cgatgctcgt taaaaaagct acccactttt cagaaatccg ttcttcaaat ggatagctac
     1921 atacctatat tactattcaa attgtcagaa gtcaatcccc aacactttat aatcctgatc
     1981 ttccactgcc tttttaagta cttcgtcagc aacctcttct gtcatttcaa caactgccgt
     2041 gccggtttca tgactgactt ccgcctgttc cactccatcc agcgcttcaa gaaccttttt
     2101 cactctcgcc tcgcagtgtc cgcacatcat tccttcgatt ttcattactt ttttcattgt
     2161 tttattctcc ttttcttctt cagaattttt cttgtttttt tgacttcttt ttctatcttt
     2221 agaaccatca tataatttaa acaaattcag cctcagggcg ttgcttacaa cacatacgct
     2281 tgacagactc attgccgctg ccccgaacat cggatttaat ttccacccga agataggaat
     2341 ccagattccc gctgcgagcg gaatgccgat cacattataa aaaaatgccc agaaaagatt
     2401 ttcatgaata ttgcgaagcg ctgcccgact catgcgcaca gcggcaggca cgtcgctcaa
     2461 cctgcttttc ataagcacaa tatccgccgc atcaatagcg atatccgttc ctgctccgat
     2521 ggcgattccg acatccgccc gcgtaagagc cggcgcatca ttaatcccgt ccccaaccat
     2581 cgccactttt ccttcttctt ttaatttttt gatcacatcc tcttttccat caggtaaaac
     2641 accggcaatc acttcatcta cacccgcctg ctctccgatc gcttttgccg tacgctcatt
     2701 atcgcctgtg agcatgacca ccttgatccc catatttttc aattctctga ccgcctgcgg
     2761 actgtcttct tttataatgt ctgcaacggc gattatgccg atcagttcct cattccttcc
     2821 gaaaaataac ggtgtttttc cttctgatgc aaaactttcc gcctgctttc ttaccgcttc
     2881 cgaaataaca gttcttcctt ctgcaaattt caagtttccg ccgaaaaatt tatcgccgcc
     2941 tatctctccc gtcaaaccat ttccggaaac tgccgtaaag tttttcgctt cttctttcgt
     3001 ctggattgct tcttcctctg cctttttcaa aattgcatgc gctaacggat gttcactttt
     3061 cttttcaaga atgtatgcat acgtcagaag ttccgactca ctaaaagccg aaccgggcaa
     3121 aatatctgtc accgtcggtc ttccttttgt gatcgttcct gttttgtcca gcactacaac
     3181 atcaattttc cccgtttctt caagagaaac tgctgtttta aataaaattc cgttctttgc
     3241 tcccacaccg tttccgacca taatcgcaac cggcgtcgca agaccgagag cacaaggaca
     3301 actgatcaca agaaccgaga ttccccgtgc aagagcaaaa ccgatcgttt ctccgaatgc
     3361 aagccataca ataatcgtca aaacagcaac tgccataaca accggaacaa acactcccga
     3421 cactttatct gccacttttg cgatcggagc ttttgtcgca gccgcatctc ccaccatatg
     3481 gataatctga gacaaagttg tgtcttctcc cactctggtc gccttacatc tcaaaaaacc
     3541 ggactggttt atcgtcgcgc ccgatacaca atctctctct tttttatcaa ccggaatact
     3601 ttcgccggta agcgctgatt cgtcaaccgc tccgcttcct tcgactacaa taccgtcagt
     3661 cggaatgctt tcccccggac gaacgacaaa cagatctccc ttttgcactt gttcgatccc
     3721 gatttttact tcctcgccgc cgcgcacaac agtcgccgtc ttaggcgcaa gacccatcag
     3781 acctttcagc gcatctgtcg tctttccttt tgaccttgct tccagaagtt taccgactgt
     3841 gatcaatgtc aggatcatcg ccgcagattc aaaataaaac tcatgcatat atgtcattac
     3901 ttctgccgca ttcccacgca tctgcgcatc tgtcatcgca aacagagcat aaatactgta
     3961 gccgtaagac gcgcctgcgc caagagcaac cagggtatcc atattcggcg ctttatgaaa
     4021 aagtcctctg actccactga taaaaaactt ctgattgatc accattacgg ctgttgtcag
     4081 cagaagctgt atgatcccca ttgccacgtg attctcgctc acaatttccg gaactttcca
     4141 tccccacatc atatgcccca ttgacagata cattaacgga atcaaaaaac aaagagaggc
     4201 gatcaatcgc tttttcatct caggcgtttc cttatctttt aattctccgc tcttttccat
     4261 cctgcctgct tcctgtcctg tcctgctttc tttctccgcc gccttatatc ccgccgcctc
     4321 tacagcttca ataactgtct gtgcatcgac agccccctct actcccatag aattagtcag
     4381 cagatttaca gaacatgacg taactccttc aatctttgat acagcttttt ctacacgtgc
     4441 actgcatgcc gcacagctca tacctgtaac ttcatattgt ttcaaccggt ttcctccttt
     4501 cgattctcaa atcctcgatt ccattgcctt ttcagaatct atttcataag ttttctcaaa
     4561 actgttacaa gttcatctat cgtctcatct tttccttccc tgatatcttc cgaaacacaa
     4621 gtacggatat gttctgataa aagaacttta ttaaaactgt tcaatgccgc ggtaatcgcc
     4681 gaaacttgta taagaatatc cggacaatac gcattattct ccaccattct gcgaatcccc
     4741 ctcacttgcc cttctattct gtttaagcgg tttatcatca attttgtttc ttcttctgta
     4801 cgctttttta tccgatgtgt acattccgga catttctttt ctttttccat aattatcctc
     4861 cgaatatatt ttccggcttt tgccttttct ccctctcacc atcatacttt aaacctaaaa
     4921 tacccatatg gggtatgtat aagtgcatta tatacccata ttaggtatat tgcaactgtt
     4981 ttttcatcga gatataaaat ttcagatctg cataacaact ttatctgttt ataaaaacat
     5041 ccataatgaa agagcaacta acttctcatc gaaattaatt gcccttttat caaacgtcat
     5101 gcaagatttt aagcaccatt cgaagctctt ctgccccgag ctgtcccggc gccgacgctt
     5161 ttttgaccgc tccgaatgta agagcagaac caaatgcttc accgcaaata cgactgacta
     5221 ctcctacttc cgacatagac attgtaacga ccggacactt tgcatgctcc ctcaccatct
     5281 cttccgttgc caaaagcagc gtaagcacat cttttttaga ttgaggcatt accgctattt
     5341 tcacaatatc cgcaccacac tcctgcataa agcaaagcct cgatacaatt tcttcttttg
     5401 gcggagtttt ttgaaaatca tggttagatg ctatcacttt tactccatac aaatgcgccg
     5461 tttctacgat tgtatttacc tcatctgtcc cggtaaacgc ttctgcatcc acaagatctg
     5521 caaaacccgt ttttactgcc atctcattca gctttacata tacactcttt tcaatctctt
     5581 tctccccgcc ttcttttaat gtgcgaaatg tgaaaaggag cggaatgtct ccaagcgtct
     5641 ctcttaattc ttccatcatc ttttctgtct ttttaaaatc cagaacatct tcataccaat
     5701 ctgcgcgcca ttccacaaga tccgccgttt ctttcgcttt acaagctgcg tcaataatat
     5761 cgtctctcgt aattccgaca acaggcacgc agattttcgg gattcctttt cctatctcta
     5821 cctttctgac atttaccgta ttcatatgct cttctctctt ttaactatat ttaaaaatct
     5881 taaccgctta taaaatctcc tgccactcct tttctttaaa accaacgaga accttctcat
     5941 tttgcacgag aatcggtctt tttacaagca tcccgtcaga agcgagcagt tcaatctgtt
     6001 catcctcact catattcgga atcctgtctt ttaactgcat ttctttgtaa agttttccac
     6061 tcgtattaaa aaagcgcttc aacggaagtc cgcttttcct ccaccacgtt ttcagttctt
     6121 ccgctgttgg tttctcttct ttgatatctc tttgctcaaa ttcaatttct ttttcttcca
     6181 gccatttttt tgctttcatg catgtactgc atttcggata acatacaaat aacattttct
     6241 ttctcctttc gtttgctact ctgacctttt ccggtctttc attttgcttt gaaaaatcta
     6301 ccgatttttt cttttataat attttgaata cagccgaatc tatttaacag tgtatctcat
     6361 ttttcctgca gatacaattc ttatttttct tatttttact cacataaata ttgcgc
//
LOCUS       DBMDXF010000106         6324 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3133_length_6324_cov_2.326049, whole genome shotgun sequence.
ACCESSION   DBMDXF010000106 DBMDXF010000000
VERSION     DBMDXF010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_6324_cov_2.326049"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            107..1159
                     /locus_tag="ACZPD4_05445"
     CDS             107..1159
                     /locus_tag="ACZPD4_05445"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1378544.1"
                     /translation="MKIIVLAGGLSTERDVSLASAAGICRTLIEKGHDAFLLDVFLGL
                     EHAPEHLEDVFTLPGHGLEIAKNISCEEPDLAAVKASRPDQSDCFLGPNVIELCRLAD
                     ITFLGLHGGEGENGKLQATFDLLGIRYTGPDSLGCAVAMDKGLTKQVFLQAGIDTPAG
                     VTIHKKSKDRSLALTGLSLPVVIKPCSGGSSIGVYIVSTEEEYAEALEKSFFYEDEVV
                     VEEYIKGREFACGVIDNEALPPIEIIPKTGFFDYANKYQADATLEVCPADIPEETASR
                     MMELTVKAFRALKLNVYSRADFLLDEKGNLYCLEMNTLPGMTAASLLPKEAKAAGIEY
                     GDLCELIIKKSLEARY"
     gene            1193..2614
                     /locus_tag="ACZPD4_05450"
     CDS             1193..2614
                     /locus_tag="ACZPD4_05450"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1378545.1"
                     /translation="MKYMSLQEIAAACGGTYYGGSEFLPREVSSVVIDSRKAEKDSLF
                     IAIRGARVDGHSFIPKVMEQGALCAVSEEDLGDVPYSYIKVASCEQALKDIAEHYRRS
                     LDIKVVGISGSVGKTSTKEMIASVLSQKYSVLKTEGNFNNEIGLPLTVFNLRKEHEIA
                     VLEMGISGFEEMTRLAKVARPDICVLTNIGVAHMENLGSRDGILKAKTEMFAYMNENG
                     TIILNGDDDKLRGYSPENGITPVYFGLDESCGFHAENISGKGLNGTDAQFFTPNSSFT
                     AHINIPGAHMVYNALAATAVGYALGMTDREICAGIEANVPIAGRNNLIRAPHFTIIDD
                     CYNANPTSMKASLDVLAFADTRKAAILGDMFELGENEKQMHREVGIHAVRKGIDVLIC
                     IGSLMKSAADGALEYLEQQKNAEHTEETPSSSAHFPTQICHFATKADFLREAKELLKE
                     GDTVLVKASHGMEFPEIVEFLQQ"
     gene            2648..3457
                     /locus_tag="ACZPD4_05455"
     CDS             2648..3457
                     /locus_tag="ACZPD4_05455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1378546.1"
                     /translation="MKYQVLVLDLDGTLTNSKKEITAPTKQALLEIQEAGRTVVLASG
                     RPINGVAPLAKELQMDTFGGYMLSFNGARITKCSTGEIIYNKTIPKEIIRPVWEYVKT
                     FPGLDIISYDDTHIFSGICPNKYVEIESGINNMEIVPTDDFPSALDFPVNKLLICGDP
                     KTLETMIEPLRKQYHGLLNIYLSEPFFLEIMPKNIDKAQSLQKLLNSIGLTANEMICC
                     GDGFNDLSMIEYAGLGVAMENAQPIIKETADFITRSNDEDGVLHVIDTFMR"
     gene            complement(3515..4390)
                     /gene="aroE"
                     /locus_tag="ACZPD4_05460"
     CDS             complement(3515..4390)
                     /gene="aroE"
                     /locus_tag="ACZPD4_05460"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR1378547.1"
                     /translation="MERRINGHTRLYALIGSPVGHSGSPAMYNYSFEKLGIDAAYLAF
                     DIPLEKTKDAIDAFRTLNVGGFNITMPNKTAAAQLVDRLSPAAELVGACNTVVVEEDK
                     TMTGHITDGIGFVRNLKEQGVGIEGKKIVLLGTGGAATAIAVQAALDKASEIAIFNRK
                     DEFFANGEKTVEKIKKAVPSMKKIYIEDIDDEKKLGEAIAGSDLLINATRAGMSPLEN
                     VLPVPEELLHKDLAVADVVYNPRETLLIKKAKEAGCKAAVGGIGMLLWQGAAAFELFT
                     GKEMPAQEVMELFFS"
     gene            4645..6075
                     /locus_tag="ACZPD4_05465"
     CDS             4645..6075
                     /locus_tag="ACZPD4_05465"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR1378548.1"
                     /translation="MKNSFSFLAKHISWKYIVLILILIYIILLTVPYLPHKTVSEEYK
                     EKDAAAEYYSDTSGTERIAYITDNNDALLYRLGMIEEAEKSIILSTFDFNDDEAGQDI
                     LSALLNAADRGVDIRVIVDGISGFMDVQHNPWFLALDAHKNAQVRIYNPVNFLKPWDM
                     QARLHDKYLIIDDQMYTLGGRNTTNLFLGDYSKGKNIDKELFVYETDPGKNMQNTSMS
                     QLQTYFDSIWDSSDSKPCRGSRNGKKTVEKTEALKKHYKELQKKYPAAYEKQNWEELT
                     FETNKITLLSNPIESENKEPWMWYSLHRLMMSGKQATIYTPYIICGREMYDDLSQLTD
                     NNVSVEIITNDVAKGANPWGCTDYLNEKEKIWRTGVKVYEYMAPHSCHTKAVLIDDRM
                     SIVGSYNLDMRSTYLDTELMLAVDSTELNAIIRKEAEHDKTFSKTMENGKYTYGENYK
                     TKELSTGKKLFYATLRQIIKPLRRFL"
BASE COUNT         1874 a         1477 c         1306 g         1667 t
ORIGIN      
        1 gacttaaccg aaacgttcca tattataatt taatgaaact aaacgaatat ctgaaaatca
       61 aataaacaaa aacgaatatc tcgaaatcaa aggagtataa acaattatga aaatcatcgt
      121 actcgccgga ggactaagca ctgaaagaga cgtttctctt gcttctgccg ccggcatctg
      181 ccgtacatta atcgaaaaag gtcatgacgc ttttcttctt gacgtatttt tagggctgga
      241 acacgctccc gaacatttgg aagacgtatt tacccttccc ggtcacggtc ttgagattgc
      301 gaaaaacatc agttgtgaag aacctgatct tgccgctgta aaagcatccc ggcctgatca
      361 atccgactgt ttcttaggac caaacgtcat tgaattatgt cgtctggcag atatcacatt
      421 tctcggactt cacggcggtg aaggagaaaa cggaaaactt caggctactt ttgacctgct
      481 cggtatccgc tacacaggtc ccgattctct cggctgcgcc gtcgctatgg ataaaggact
      541 gacaaaacaa gtctttctcc aggcaggcat cgacacgccg gcaggcgtta ccattcacaa
      601 gaaatcaaaa gaccgctctc ttgctttaac aggtctttcc cttcctgtcg ttataaaacc
      661 atgttctgga ggttcgagca tcggggtata tatcgtcagt acagaagaag aatatgcaga
      721 agctttggaa aaatcctttt tctatgagga tgaagtcgtt gtagaagagt atataaaagg
      781 acgtgaattt gcatgtggcg taattgacaa cgaggcgctt cctccgatcg aaatcatacc
      841 gaaaacagga tttttcgatt atgcgaataa atatcaggcg gacgcgactt tagaagtatg
      901 ccctgcggac attcccgaag agacagcttc ccggatgatg gaacttaccg taaaagcatt
      961 ccgtgcgctg aaactcaacg tatacagccg cgccgatttt cttctggatg aaaaaggaaa
     1021 tctttactgt ctggaaatga acactctgcc gggcatgacc gcagccagcc tgctgccgaa
     1081 agaagcgaaa gcggcaggaa tcgaatacgg cgatctgtgt gaactgatca tcaaaaaatc
     1141 gttagaagcg cgttattaat acatgttgtt taagaaaggc aggagctatc ttatgaaata
     1201 tatgtcatta caggaaattg ccgcagcctg cggcggtaca tactacggcg gatctgaatt
     1261 tctcccccgt gaagtaagca gcgtcgtcat cgacagccgt aaagcagaaa aagacagtct
     1321 gtttatcgcg atccgggggg cacgcgtcga cggacattcc tttattccga aagtaatgga
     1381 acaaggagcg ctctgcgccg tttccgaaga agatctcgga gatgttcctt attcttatat
     1441 taaagtggcg tcatgtgaac aggctttaaa agatatcgca gaacactatc ggcgttccct
     1501 tgatatcaaa gttgtaggca tttcgggaag cgtcggaaaa acgagcacaa aagaaatgat
     1561 cgcctctgtc ctgtcacaaa aatattcggt tctgaaaaca gaaggaaact ttaacaatga
     1621 gattgggctt cctcttactg ttttcaacct ccgtaaggaa catgaaatcg cggttcttga
     1681 gatggggatc agcggcttcg aagagatgac gcgccttgcg aaagttgccc ggccggacat
     1741 ctgcgttctc acaaatatcg gtgtcgccca tatggaaaat ctaggttcaa gagacgggat
     1801 cttaaaagca aaaacagaaa tgtttgctta tatgaatgaa aacggaacaa taatcttaaa
     1861 cggagacgat gacaaactgc gcggatattc tcccgaaaac ggaatcacgc ctgtctactt
     1921 cggtcttgat gaaagctgcg gttttcacgc cgaaaatatt tccggcaaag gtttaaacgg
     1981 aacggacgca cagtttttca caccaaattc ttcttttacg gcacatatca acatcccggg
     2041 cgctcacatg gtgtacaacg cgcttgcggc taccgcagtc ggatacgctc ttggtatgac
     2101 agacagggaa atctgcgcag gaatcgaagc aaatgttccg attgccggac gcaataacct
     2161 gatccgtgcg ccccatttta cgatcatcga cgactgctac aacgcaaacc ctacttctat
     2221 gaaagcgtct ctcgatgttc ttgcctttgc agatactcgc aaagcagcaa tactcggaga
     2281 tatgtttgaa ctcggagaaa acgaaaaaca gatgcaccgc gaagtcggca ttcacgccgt
     2341 cagaaaaggc atcgatgttt tgatctgcat cggcagtttg atgaagtcgg cggcagacgg
     2401 agcgttggaa tatcttgagc agcaaaaaaa cgcagaacat accgaagaga cgccttcgtc
     2461 ttctgcgcat tttccgactc agatctgcca tttcgccaca aaggcagatt ttctgcgcga
     2521 ggcgaaagaa cttttaaaag aaggcgatac cgtacttgta aaggcttcac acggaatgga
     2581 gtttccggaa atcgtcgaat ttttacagca ataatattat attttgataa agcataggag
     2641 aaggattatg aaatatcagg ttctcgtcct tgatctggac ggcactttga ccaattcaaa
     2701 aaaggaaatt accgcgccga caaaacaggc gcttcttgag attcaggaag ccggccgcac
     2761 tgtcgttctt gccagcgggc ggccgatcaa cggtgttgcg cctcttgcaa aagaacttca
     2821 gatggatact ttcggcggat acatgctctc ctttaacggc gcacgtatta caaaatgttc
     2881 cacaggcgag atcatttaca ataaaacgat cccaaaagaa atcatccgtc cggtctggga
     2941 atacgtaaag acatttccgg gactggatat catctcttac gatgacactc atattttctc
     3001 agggatttgt ccgaacaaat atgtagaaat agaatcggga ataaacaata tggaaattgt
     3061 tccaacggac gactttccat ccgcccttga tttcccggta aataagctgc tcatctgcgg
     3121 cgatccgaaa acacttgaaa cgatgatcga accgctccgc aagcagtacc acgggctttt
     3181 aaatatctat ctttccgaac cgtttttctt ggagatcatg ccgaaaaaca ttgacaaagc
     3241 gcagtctctc caaaagctgt taaacagcat cggtctgacc gctaatgaga tgatctgctg
     3301 cggcgacggc ttcaacgatc tgtccatgat cgaatatgcc ggtcttggag tcgccatgga
     3361 aaacgcacag ccgatcataa aagaaaccgc cgacttcatc actcgttcca atgacgaaga
     3421 cggcgtactc catgtgatcg atacgtttat gcgataaaga aatcttttat atcggctcac
     3481 acatacaatc tgtaagagct gtcacgatac tttttcagga aaagaaaagt tccatcactt
     3541 cctgtgccgg catttctttt cctgtaaaca gttcaaacgc cgcagctccc tgccagagca
     3601 gcatcccgat tcctccgact gcggctttac agcctgcctc ttttgctttt tttataagca
     3661 gcgtctctct cggattatac acgacgtccg caacagccag atctttatga agtaattctt
     3721 ccggaacggg aagcacgttt tccaaaggac tcattccggc gcgtgttgca ttgatcaaaa
     3781 gatcgcttcc ggcgatagct tctcctaatt ttttctcatc atcaatatct tcaatataga
     3841 tttttttcat cgacggtact gcttttttaa tcttttctac cgttttttct ccgtttgcga
     3901 aaaattcatc cttccggtta aatatcgcaa tctctgacgc tttatcaagc gccgcctgta
     3961 ctgcgatcgc agtcgccgct ccgcctgtac caagcaaaac gatcttcttt ccctctatcc
     4021 cgactccttg ttccttcaga ttgcggacaa atccgatccc gtctgtaata tgccctgtca
     4081 tcgttttgtc ctcttccaca acgacggtgt tacacgctcc gacaagttcc gccgcagggg
     4141 acaatctgtc tacaagctgt gccgccgcgg ttttgttcgg catcgttatg ttaaaaccgc
     4201 ctacgtttaa tgtgcggaat gcgtcaatcg catcttttgt tttctccagc ggtatgtcaa
     4261 acgcaagata cgccgcatcg atccctagct tttcaaaact gtagttgtac atagccggag
     4321 accccgaatg gcctaccggt gaaccgatca gcgcgtaaag ccttgtatgt ccgttaattc
     4381 ttctttccat cttttctccc tccatgatca tgcgctctca aaacaggcgc actgcaaata
     4441 tggatacagt atatcacaac tttttgttta cttcaacact ttaaagtgtg aataaaattc
     4501 gttaaaataa aaatcgaatt aaaatgcata catttatccc gtgtcacccc atgttactaa
     4561 cgcgttgtcg ttttgtctta atgaattgcc ccttttaaaa aaatgtgcta tcttattcca
     4621 aaagaaagga gattttacga aaatatgaaa aacagttttt cttttctcgc aaaacatatt
     4681 tcatggaaat acattgtact tatcttaatc cttatttata ttatcctcct tactgttcct
     4741 tatctgccgc ataaaactgt atcggaagaa tacaaagaaa aagatgcggc agccgaatat
     4801 tattccgaca catccgggac agagcgcatc gcctacatca ctgataataa cgacgctctt
     4861 ttgtatcggc tcggtatgat cgaagaagca gaaaaatcca tcattttatc gacctttgat
     4921 tttaacgatg atgaggcggg gcaggatatt ctttccgcct tactaaacgc cgcggaccgg
     4981 ggcgtcgata tccgtgttat cgtcgatgga atcagcggat ttatggatgt acagcacaat
     5041 ccgtggtttc ttgctttaga cgctcataaa aacgctcagg tccgcattta taatcctgtg
     5101 aattttttga aaccatggga tatgcaggcc cgccttcatg acaaatatct gatcatcgat
     5161 gaccagatgt atacacttgg cggacgcaac acaacaaacc tgtttctcgg agattattca
     5221 aaaggaaaaa acatcgacaa ggagctgttt gtttacgaaa cagatccggg caaaaatatg
     5281 caaaacactt ctatgtccca gcttcagact tattttgatt ccatatggga ttcttctgac
     5341 agcaaaccgt gccgtggctc aagaaacgga aaaaagacgg tcgaaaaaac agaagctctg
     5401 aaaaaacatt acaaagagct acagaaaaaa tatcctgccg catacgaaaa acaaaactgg
     5461 gaagaactga catttgagac aaataagatc actcttttga gcaatccgat cgaatcagaa
     5521 aacaaagaac cgtggatgtg gtactctctt caccggctta tgatgtccgg aaaacaggcg
     5581 acgatctaca ccccttacat tatatgcggc agggaaatgt acgacgatct ttctcagctc
     5641 acagataata acgtctctgt cgagatcatt acaaatgatg tcgcaaaagg cgccaatccg
     5701 tggggctgta cagattactt aaatgaaaaa gaaaagatat ggagaaccgg tgtgaaagtt
     5761 tacgaatata tggctcccca ttcctgccat acgaaagccg ttctcataga tgaccgcatg
     5821 agtatcgtcg gttcttacaa tcttgacatg cgaagcacat atcttgatac cgaattaatg
     5881 cttgccgtcg attcaaccga attaaacgca attatacgaa aagaagccga acatgataaa
     5941 accttttcaa aaacgatgga aaacggaaaa tatacctacg gggaaaatta caaaacaaaa
     6001 gaattaagca ccggaaagaa attattctac gcaacattaa gacaaattat caaaccgctc
     6061 cgaagattct tatagacaac tttattaaaa actctttata gacaaaactt ttatgattaa
     6121 atttttacag ccgaatttct tatgatcgat atcttctctg atcggattta tctgcacgga
     6181 aacgagaatc acaaatcagg catcccttgt accttcccga gaactgttcc catgcagata
     6241 aaccggtctt tcggctttac tactacatcc tgttccaata cgttcaatgc tttcagcttt
     6301 ttctcttcat tttcctgata taat
//
LOCUS       DBMDXF010000107         6302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3146_length_6302_cov_2.024028, whole genome shotgun sequence.
ACCESSION   DBMDXF010000107 DBMDXF010000000
VERSION     DBMDXF010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3146_length_6302_cov_2.024028"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /gene="ppdK"
                     /locus_tag="ACZPD4_05470"
     CDS             complement(<1..1801)
                     /gene="ppdK"
                     /locus_tag="ACZPD4_05470"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR1378549.1"
                     /translation="MAKWVYMFTEGNATMRNLLGGKGANLAEMTSLGLPVPQGFTVTT
                     EACTQYYEDGRKINDEIMSQIMDAIVKLEEITGKKFGDKENPLLVSVRSGARASMPGM
                     MDTILNLGLNEEVVETLAEASGNARWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKMK
                     EEKGVTQDVDLTAEDLKTLAGQFKAEYKEKIGADFPSDAKEQLMGAVKAVFRSWDNPR
                     ANVYRRDNDIPYSWGTAVNVQMMAFGNMGDDCGTGVAFTRDPATGENGLFGEFLTNAQ
                     GEDVVAGVRTPMHISEMEQKFPEAFAQFKDVCKTLETHYRDMQDMEFTVEHGKLFMLQ
                     TRNGKRTAQAALKIACDLVDEGMRTEEEAVAMIDPRNLDTLLHPQFDAAALKAATPMA
                     KALGASPGAACGKIVFTADDAVEWAARGEKVILVRLETSPEDITGMKSAQGILTVRGG
                     MTSHAAVVARGMGTCCVSGCGDITMDEANKKFTLAGKEFHEGDSISLDGSTGAIYDGI
                     IPTVDATIAGEFGRIMGWADKYRTMKVRTNADTPADAKKARELGAEGIGLCRTEHMFF
                     EGNRIDAFREMICSETVEEREAALEKILPEQQGD"
     gene            complement(1884..3149)
                     /locus_tag="ACZPD4_05475"
     CDS             complement(1884..3149)
                     /locus_tag="ACZPD4_05475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1378550.1"
                     /translation="MNYVIIGTGAAGIMAAKEIRKLKKDAQITMISADEQVHSRCMLH
                     KYLSHERTAEELSFVEEDFFEKNQIAEVYGHIEVIDTANQMVDTKGGESICYDKLLIS
                     TGADSFIPPVGDLRKAKNVFGLRHLKDAQEIDKMAEDAEKILIIGSGLVGLDAAYGLI
                     ERGKKVTVVEMAEQILPVQLDAHAAKTYQELFEQAGVQFYLGCKAEGAVCEADGMIRA
                     VTLDTGKQLLCDLIIVAAGVRPAVGFLEKSEIEVERGIKVNSKMETNVPNVYAAGDVT
                     GLSGIWPNAMKQGQTAARNMCGVGTEYTDTFAAKNTINFFGLVTLCLGRIRQEEGDEI
                     FVEEDRNVYRRLIMKDGVPEGILLQGDIAHSGIWQYMIKSKIDISSMKNKIFKITFAD
                     FYGIGDRGKYVWKIDEKMDKKTDKSRQNA"
     gene            complement(3153..3599)
                     /locus_tag="ACZPD4_05480"
     CDS             complement(3153..3599)
                     /locus_tag="ACZPD4_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1378551.1"
                     /translation="MRRIIVEYEKCDGCKNCSAACMQAHRKTPGTIYDLDLTDPENES
                     RNYILKGDDGTYRPLFCRHCDEPECVMSCMSGALQKDPETGIVTYDETKCGSCFMCVM
                     NCPFGVLEPDRMTKKKVIKCDFCMSDGQEPNCVKSCPKGAIHVEEV"
     gene            complement(3612..5516)
                     /gene="cooS"
                     /locus_tag="ACZPD4_05485"
     CDS             complement(3612..5516)
                     /gene="cooS"
                     /locus_tag="ACZPD4_05485"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HIR1378552.1"
                     /translation="MNQCYGCTTCESADKPLEGFIKNLPLETSHHRVEGQSTKCAFGL
                     QGVCCRLCSNGPCRITPDAPRGICGANADTIVARNFLRAVASGSGCYIHVVENTARNV
                     KNAAQKKSGIKGEGALNKLAALFEIEEEDMYVRAEKVADAVLADLYLPEYEKMKLVKK
                     MAYAPRYENWEKLGILPGGAKSEVCHGVVKCSTNLNSDPVDMLKDCLKLGISTGIYGL
                     TLTNLLNDIVLGEPKLRLAPVGLRVIDPDYINIMITGHQHSMFTYLQERLTDADITEK
                     AKQAGAKGFKLVGCTCVGQDLQLRGAHYEDVFDGHAGNNYTSEAILATGAIDAVISEF
                     NCTLPGIEPICDELKIKQLCIDSVAKKANAELEEFDFENREQVTEEIIDKVLLSYKER
                     RGADCASEERVELNLMEEHGNERTLTGVSEGSLKEFLGGNWKPLVDLIVSGDIKGVAG
                     VVGCSNLTAGGHDVLTVELTKELIARDIIVLTAGCSSGGIENCGLMTPEAAQFAGPKL
                     RAVCEKLGIPPVLNFGPCLAIGRLEIVATELAETIGVDLPQLPLVLSAAQWLEEQALA
                     DGCFGLALGLPLHLGLPPFVTGSGAAVKILTEDMKELTGGQVIINPDAKESADILEKI
                     ILEKRDALHI"
     gene            complement(5665..6060)
                     /locus_tag="ACZPD4_05490"
     CDS             complement(5665..6060)
                     /locus_tag="ACZPD4_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1378553.1"
                     /translation="MLFTKESDYAIRVIRALQDGGKKTIKELCELEQIPSAFGYKILQ
                     KMNKSGLIEIRRGPKGGYQLAKDIGEFTLYDVVTAIEPDFAVMECVHHSCIRNESKEG
                     CAVHRELLGIQHSVEKLLKKKTMADILKK"
BASE COUNT         1514 a         1711 c         1114 g         1963 t
ORIGIN      
        1 agtcaccctg ctgctccgga aggatttttt caagagccgc ttctctttcc tctactgttt
       61 cagagcagat catctcacgg aaagcgtcga ttctgttgcc ttcaaagaac atatgctctg
      121 tacggcaaag tccgatacct tctgctccaa gttcgcgggc tttcttagcg tcagccggtg
      181 tatctgcgtt tgtacgaact ttcattgttc tgtatttatc agcccatccc atgattcttc
      241 cgaactcacc tgcgatcgta gcatctactg tcgggatgat tccgtcataa atagctcctg
      301 tagatccgtc aagagaaatg ctgtctccct cgtggaactc ttttcctgct aatgtgaatt
      361 tcttgtttgc ttcatccatt gtgatgtctc cgcatccaga tacacagcat gtacccatac
      421 cacgtgcaac aacggctgcg tgagatgtca taccaccgcg aactgtcagg ataccctgtg
      481 cggatttcat acctgtgatg tcttccggtg atgtctcaag acgaacaagg attacttttt
      541 ctcctcttgc agcccattca acagcgtcgt cagctgtgaa cacgatctta ccgcaagcag
      601 ctcccggaga tgctccaagt gcttttgcca tcggtgttgc ggctttcagt gcggcagcat
      661 cgaactgcgg atggagaagt gtatcaaggt ttctcggatc gatcatagct acagcttctt
      721 cttctgttct cattccctcg tcaacaaggt cacaagcgat cttaagagct gcctgagctg
      781 ttctcttacc gttacgagtc tgaagcatga acagtttacc atgctctaca gtaaactcca
      841 tgtcctgcat atctctgtag tgtgtttcaa gtgttttgca cacatctttg aactgtgcga
      901 acgcttccgg gaatttctgt tccatctcgg aaatgtgcat cggtgtacga acacctgcaa
      961 ctacgtcttc gccctgtgca tttgtaagga actctccgaa aagtccgttc tctcctgtag
     1021 cagggtcacg tgtaaatgca acacctgtac cacagtcatc tcccatattt ccgaatgcca
     1081 tcatctgtac gttgacagca gttccccatg aatacgggat atcattgtca cggcggtata
     1141 cgtttgctct cgggttatcc catgaacgga atacagcttt aacagctccc attaactgct
     1201 cttttgcgtc tgacgggaaa tctgctccga tcttctcttt gtactcagct ttaaactgtc
     1261 cggcaagagt cttaagatct tctgctgtaa gatcaacgtc ctgagtaacg cctttttctt
     1321 ctttcatttt gtcgataagc tcttcaaagt atttcttacc aacttccata actacatcag
     1381 aatacatctg gatgaatctt ctgtagcagt cccatgccca acgagcattt ccggaagcct
     1441 ctgcaagagt ctctacaact tcttcgttaa gaccgaggtt aagaattgta tccatcatac
     1501 ccggcatgga agctcttgct ccggaacgaa cggaaacaag aagaggattc tctttatctc
     1561 cgaatttctt tcctgtgatc tcttccaatt taacaatagc gtccatgatc tgactcataa
     1621 tttcgtcgtt gatctttctg ccatcttcat aatactgtgt acaagcctct gttgttacag
     1681 taaatccctg cggtacaggg agtccgagac ttgtcatctc tgcaaggttc gcgccttttc
     1741 caccaagaag gtttctcatt gttgcgttac cttctgtgaa catataaacc cattttgcca
     1801 tgtcacaatg acctcctaaa aaattttgca aatatttctg ctataattcg catataatat
     1861 tctaatggtt ttattcactt gcgtcaagcg ttttgtcggc ttttgtctgt ctttttgtcc
     1921 atcttttcgt ctattttcca cacatatttc ccacgatctc cgattccgta aaaatcagca
     1981 aacgtaatct taaaaatctt atttttcata gaactgatat ctatcttact tttgatcata
     2041 tattgccata ttcccgaatg cgcaatatct ccctgaagca aaattccttc cggtactccg
     2101 tctttcatga tcagacggcg atatacgttt ctgtcttcct cgacaaagat ctcatcccct
     2161 tcttcctgtc tgatccttcc gaggcaaaga gtaaccagtc cgaaaaagtt gatcgtattt
     2221 ttcgcggcaa atgtatccgt atactctgta ccgactccgc acatatttct tgccgccgtc
     2281 tgtccctgct tcatcgcgtt cggccagatg cccgacaatc ccgtaacatc tcccgccgca
     2341 tatacgttcg gtacatttgt ctccatctta gaatttactt tgattcctcg ttcgacctcg
     2401 atctcacttt tttctaaaaa cccgactgcc ggacgtacgc cagccgccac gatgatgaga
     2461 tcgcataaaa gctgttttcc cgtatcaaga gtaaccgcac gaatcatacc gtcagcttcg
     2521 cataccgcgc cttcggcttt gcatccgaga taaaattgta cccctgcctg ttcaaacagt
     2581 tcctgatatg tcttcgccgc atgtgcgtca agctgtacgg gaagaatctg ttccgccatc
     2641 tccacgacag tcaccttttt ccctcgctcg atcaatccgt acgctgcatc cagtccgaca
     2701 agtcctgatc cgataatcag aattttttcc gcatcttccg ccattttatc gatctcctgc
     2761 gcgtctttca aatgccggag tccaaataca tttttcgctt tccgaagatc tcctacagga
     2821 ggaataaaac tgtctgcccc tgtggaaatg agcagtttgt cataacagat cgattctcct
     2881 ccctttgtat caaccatctg atttgccgta tcgataactt caatatgtcc gtatacttcc
     2941 gcgatctgat ttttctcaaa aaagtcttct tccacaaacg acaactcttc cgccgtcctt
     3001 tcgtgggaaa gatatttgtg cagcatacag cgggaatgaa cttgctcatc tgccgaaatc
     3061 atcgtaatct gtgcatcttt tttcagcttt ctgatctctt ttgcggccat gatccccgcc
     3121 gctcctgttc cgataatcac gtagttcatc catcacacct cctctacatg gatcgcgcct
     3181 ttcggacacg atttcacaca gtttggttcc tgaccgtcag acatacaaaa atcacacttg
     3241 atcacttttt tctttgtcat ccgatccggt tcaagaacac caaacggaca attcatcaca
     3301 cacataaagc aagatccgca ttttgtctcg tcatatgtca cgatccctgt ttccgggtct
     3361 ttctgaagcg ccccgctcat acatgacatt acgcattccg gctcgtcaca atgcctgcag
     3421 aacaaaggtc tgtatgtgcc gtcgtctcct tttaaaatat aatttctcga ttcgttttcc
     3481 ggatcggtca gatccaaatc ataaattgtt cccggcgttt tcctgtgcgc ctgcatacat
     3541 gctgccgaac aattcttaca gccgtcgcat ttttcatatt ctacaataat ccttctcatg
     3601 gagccgccct cctatatatg aagcgcgtcg cgcttttcca gaataatctt ttccaaaata
     3661 tcggcagatt cttttgcgtc cggattaatg ataacttgcc cgcctgtcaa ttctttcata
     3721 tcttctgtca atatttttac agccgcgccg cttcccgtta caaacggcgg aagtcctaaa
     3781 tgaagcggaa gtcctaacgc cagaccgaag cagccgtccg caagagcctg ctcctcaagc
     3841 cactgcgccg ccgaaagcac gagcggaagc tgaggcagat cgacgccgat cgtctccgca
     3901 agttccgtcg ccacgatctc caaacgtccg atcgcaaggc acggtccgaa attaagcacc
     3961 ggaggaatcc ccagtttttc acatactgcg cgaagtttcg gtcctgcaaa ctgtgcggct
     4021 tccggcgtca tcaaaccgca attttcaatt cctcccgaag aacatcctgc cgtcaaaacg
     4081 ataatatccc gggcaatcag ctctttcgta agttctaccg taaggacatc atgtcctccc
     4141 gccgtcaagt ttgaacagcc cacaactccc gcaactcctt taatatcgcc tgatacgatc
     4201 agatctacaa gcggcttcca atttccgccg agaaattctt tcaaggaacc ttcactgact
     4261 cctgtaagtg tacgttcatt gccatgctct tccatcagat tcagttctac ccgttcttcc
     4321 gacgcacaat ccgcgcctct tctctcttta taagaaagaa gaactttatc tataatttcc
     4381 tccgttacct gctctctgtt ttcaaagtca aattcttcca gctctgcatt tgcttttttc
     4441 gccacactgt caatacaaag ctgtttgatc ttcaattcgt cacagatcgg ttcgattccc
     4501 ggcaaagtac agttaaattc cgaaatcact gcatcgattg cgcctgtggc gagaatcgct
     4561 tcgctcgtgt agttatttcc ggcatgtccg tcaaacacat cttcataatg cgcgcctcga
     4621 agctggagat cttgtccgac acaagtacat ccgacaagct taaacccttt tgctcccgcc
     4681 tgttttgcct tttccgtaat atccgcatct gtaagccttt cctgaagata tgtaaacatc
     4741 gaatgctgat gtcctgtaat cataatattg atataatccg gatcgatcac cctcagaccg
     4801 accggagcca gacgaagctt cggttctccg agcacgatat cattcagaag attcgtcaat
     4861 gtcagcccgt aaatacctgt agaaattcca agtttcaaac aatcttttaa catatcgacc
     4921 ggatctgaat ttaagtttgt cgaacatttg acaactccat gacagacttc cgattttgct
     4981 ccgcccggaa ggatgccgag tttttcccag ttttcataac gaggcgcata tgccattttc
     5041 tttacaagtt tcattttctc atattcggga agatacagat ccgcaagaac cgcatcggca
     5101 actttctctg cccggacgta catatcctct tcttcaatct caaaaagagc cgccagttta
     5161 tttaatgctc cttctccttt tatcccactc tttttctgcg ctgcattttt cacatttctt
     5221 gccgtattct ctacgacatg gatatagcac cccgagccgg acgctaccgc acgaagaaag
     5281 tttctcgcaa cgatcgtatc tgcatttgca ccgcagattc ctctcggggc atccggtgtg
     5341 attctgcacg gtccgtttga acaaagccga caacatactc cttgaagtcc aaaagcacat
     5401 tttgtgctct gaccttccac tctatggtga gacgtttcca gcggaaggtt tttaataaac
     5461 ccctccaacg gcttatccgc actttcacaa gtcgtacatc cataacattg attcatctgt
     5521 tcttcctcct taacacttca tataaatagt tttctgctgc gcacgtgagt ctgttttaat
     5581 ggatattttt agtccacttt ttgtcggaaa gaaaagtggg catacaccca ctttgtcatc
     5641 tgtgtatcat tttctacaca gatgctactt ttttaaaata tcagccattg tctttttttt
     5701 caaaagcttt tctaccgaat gctgaattcc aagaagttct ctgtgaaccg cacatccctc
     5761 tttgctttca tttctgatac aagagtgatg aacacattcc ataaccgcaa agtccggttc
     5821 gattgccgtt actacgtcat acaatgtaaa ttcgccgata tcttttgcaa gctgataccc
     5881 gccctttggg cctctgcgta tttcgatcaa tccggatttg ttcatctttt gaagaatctt
     5941 ataaccaaac gccgatggta tctgttctaa ttcacaaagt tcttttatcg ttttcttacc
     6001 gccatcttgt aaagctctta taacacgaat ggcataatca ctctctttcg taaagagcac
     6061 atcatcacct gctttgttag aattatatca atgtagagta tttttgtcaa caatccattc
     6121 tacactttca cctgttttat ttcttatttt atatattttt tgtaactttt cacatcatca
     6181 gaatgttttt tactgatgtt aatataattt cttatatatt ctaaaaatat attcccgtgt
     6241 atttgttcga ttattccata cgcaggaatg ctctatatcc tttcacagcc tcatacacct
     6301 cg
//
LOCUS       DBMDXF010000108         6188 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3227_length_6188_cov_2.011828, whole genome shotgun sequence.
ACCESSION   DBMDXF010000108 DBMDXF010000000
VERSION     DBMDXF010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_6188_cov_2.011828"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="ACZPD4_05495"
     CDS             complement(<1..746)
                     /locus_tag="ACZPD4_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HIR1378554.1"
                     /translation="MAKWIIGKAEKSEAYRAGTLTGMKHPKPDREMIKAAGGLPELIR
                     QADELEKSGYIRTEKSNLGADMKKIYYSIDVIPKLCEKEGIEDPRKQQLRYIKQIEQL
                     RAEVQGSFLEGYYDEIIRRLELGEIVKSPDMEDVDFFRCLNAVVNLKKSLWMRVFSAA
                     VLNDSKRFKKDYEKKVVKVLVRSPLYEEGMTDDEILSVHGILSYAQVMEWKGPLLYKL
                     KGGQEYIEDKAREKEYEIDTSQNQYGTVINS"
     gene            complement(829..4194)
                     /locus_tag="ACZPD4_05500"
     CDS             complement(829..4194)
                     /locus_tag="ACZPD4_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1378555.1"
                     /translation="MKQPKKIFTRMLINNWGGISHKMLEFHEYVNLFSGKSGSGKSTV
                     MDAIQVVLYGSVSANFLNKAADDSKNKRSVLSYLRGAQKDGTANRGDVDFCSQIVLEI
                     EDTATHIVTCVGAAFEVAKGDTDLKKYTYFSHSGRIPKDEYLENNVPYSIAQIRKLTE
                     ERSRSADNRGRGEVNKVYPSKEAYLYTLYGEIFGQVEQGRMITMEKSAIALKMTSGTG
                     QFIRDYMFPKTKEDTVATISEQLGAYREIKERVEDLEKRIEMLDKIQVYDRELTNVRA
                     EKVRTETVLKCIDIEENIIRLKGRESELESVEKEADEAKKEQKILQDELQTIRDERAR
                     VQAELNNSDYGKKEQELKDLEYRIALLADNSAQWRQIVKGLKRWEEEDVVTDYVSNRA
                     LQLIQEIRGGALTEKLCKELRTHLDLVLDNITNELSELRISIHETEKDIKEKQDAVED
                     LQNDRKPYPLELKKARQELQRALSDRYGRKIEVHIFADLFDITDEKWKNAIEGRLGRI
                     KKSMITEPAYALDAAKIFRRMQQFEEVDLINSEAVQKDSPEADENSLYEAVHAESGYV
                     DRCLKRYLGRIQKCETVEELSTVKDGVTPDCYSYSGYMFRHLRRKDYTKNACIGTKVS
                     KAKLRELQEEIIDLQSSLIADRQTEESLKEARQFERFGQEPEHLMRLAGAADELADHY
                     RRQDRLKEEIKELKNGTLAAELQAKTKELTSRIEEKEQAAGRLREKEIETGKKKGKLE
                     EKIKALKERLDFLMKGYVPNDEIFAEVRSALETKSEAAYKKEMTVMAERLFEKETKLQ
                     DERIKSRNAFNNTYPAYGFSGVEHDNEVYDKVLERCRKDFEPKYKEEFEKQYELVYHS
                     LRDNVIATIHGEIKSAYRHRRQINKMLSQIRFSDSIYQIDISPAKNENGQFYEMLMAE
                     ELDSKVIDHSGFDGQMSLMEDEFFRKYERKIGLLTEKFMPSREEDGAGREKHKNEMER
                     FADYRNYLDFNMYEQSIDENGIEKKNYVDDMAGVDSGGEGQNPKYVALLAGFAMLYMQ
                     QSNRDSKIRLVLLDEAFSKMDKERSEVCLKYARELELQLIVCVPDERLQSLIRNVDSV
                     YGFRRFRNQVTMMHIDKGNYLDMIAGESRSEEV"
     gene            complement(4151..4789)
                     /locus_tag="ACZPD4_05505"
     CDS             complement(4151..4789)
                     /locus_tag="ACZPD4_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HIR1378556.1"
                     /translation="MFEYLESISITEGEQIKKTIQALFRQTCILQVKYDPATLVPKDN
                     VQYEICVRHRHFIEDYLSVLGCELTHDAQEHIFCLTGDGAAREKLNMTETVILLIIKL
                     IYKDKIMGEGLNAPVTTLKEMREYGKNTNLIVRKLKADEWKASLYLMRLHQIIDVPGA
                     VRDVEDDTPIYIYSTINLYVTAKDMNDLIEEYREEADGYETAEENFYKNADQ"
     gene            complement(4793..>6188)
                     /locus_tag="ACZPD4_05510"
     CDS             complement(4793..>6188)
                     /locus_tag="ACZPD4_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HIR1378557.1"
                     /translation="YMLFLTAIYEEMSLSYSVLGLTEKECRAVMNEKIAAAALLWDEE
                     NYDEEGEFLTRSNMASVCLKHFQEWGWLKQDYDEALNSYVVTFPEYSQMYVELFRSLL
                     DEQESEERESVMTIYSHLYTYSSDKEKNNEILKSALRVSKKLVQMLVNMQDGMRGYFD
                     ELSRQKDFRGIQDVLVKEINNSDSRKYAILTTTDSFYRYKEAVKELLDRNLQENDMSR
                     LKLLRELEEVRQKKMKDEENAGKVKDPKKTDEAEKIKELRLERAAAMCEEAADTLIRI
                     ERQFDAIERRYNKLIEQKTIFASRAAARIRYVLQEGAEEDRTVALVDLLNKSRRKEEI
                     SEKLAERIRLSRPHRALTDESLYKRKEKGREAFVPATVSEGGQAEENLESFVLKPLYT
                     RKELQEFKERNSVGNVFRTTRQTVRSAEDLEKLFFIWQEATENAFGSGEITVGEEEET
                     EQGMRFSTLEIRED"
BASE COUNT         1401 a         1690 c          957 g         2140 t
ORIGIN      
        1 gaattaatca ctgttccgta ttgattttga gatgtatcta tttcatattc cttttccctc
       61 gccttatctt ctatatactc ctgtccgccc tttaatttat acagaagcgg acctttccat
      121 tccatcacct gtgcataact gagaattcca tgaacgctta atatctcatc gtcagtcatc
      181 ccctcttcat acaaaggtga tctgaccagt actttcacca cttttttctc ataatctttt
      241 ttaaaacgct tagagtcatt aagcacagca gcactgaata cgcgcatcca cagagatttt
      301 ttcaaattca caactgcatt caaacatcgg aaaaaatcaa cgtcctccat atcgggtgat
      361 tttacgatct ccccaagttc caaccgtctt atgatctcat cataataacc ttccaaaaaa
      421 ctgccttgta cctcagcgcg cagttgttct atttgtttta tataacgaag ctgctgcttt
      481 cttggatctt caattccttc tttttcgcac agctttggaa tgacatctat ggaataatag
      541 atttttttca tatccgcgcc cagattgctt ttttctgtcc ggatatatcc gcttttctcc
      601 aactcatcag cctgtctgat cagctcaggc aagccacctg ctgccttgat catttctctg
      661 tccggtttcg gatgtttcat gcctgtcagc gttcccgccc gataagcttc acttttttcc
      721 gctttcccaa taatccactt tgccaacaaa ttgccgctca tatgcgattc aacattttcc
      781 ttccttttta tcaactttta actattgctc catcatccaa aaacatttct acacctcttc
      841 cgatctgctt tcccctgcga tcatatcgag ataatttcct ttgtcaatat gcatcatcgt
      901 aacctgattt ctaaacctgc ggaagccgta tacgctgtcc acatttcgga tcagagactg
      961 aagtctttca tccggcacgc agacaatcaa ctgaagttcc aattctcttg cgtattttaa
     1021 gcagacttcg cttcgctcct tatccatttt tgaaaatgcc tcatcaagaa gaacaaggcg
     1081 aatctttgaa tcccgattac tctgctgcat atagagcatt gcgaatcctg caagaagcgc
     1141 cacatatttc gggttctgtc cctctcctcc ggaatcgacg cccgccatgt cgtccacata
     1201 atttttcttc tcgatcccgt tttcatcgat agattgttca tacatattaa agtccaaata
     1261 gtttctgtag tcagcaaagc gttccatttc atttttgtgc ttttcacgcc ctgccccgtc
     1321 ttcctctctg gacggcatga atttctcggt aagaagtccg atcttccttt catattttct
     1381 gaaaaattca tcttccatca aactcatctg accgtcaaac ccactatgat ctattacttt
     1441 gctgtccagt tcttctgcca taagcatctc ataaaactgt ccgttttcat tttttgccgg
     1501 agagatatca atctgataaa tgctgtccga aaaacggatc tgcgacagca ttttattgat
     1561 ctgacgccga tgtctgtatg ccgacttgat ttccccgtgt atcgttgcga tcacgttatc
     1621 gcgcagagaa tggtacacga gctcgtattg tttttcaaat tcttctttat atttcggctc
     1681 aaaatctttt ctgcatcgct ccaaaacttt gtcgtaaact tcattgtcat gttctacacc
     1741 tgaaaatcca tatgcgggat atgtattgtt aaatgcattt cttgatttta tccgctcatc
     1801 ctgaagtttt gtttctttct caaaaagcct ctccgccatc actgtcatct cttttttata
     1861 agccgcttcg ctcttcgttt ccaaagccga cctcacttcg gcaaatatct catcattcgg
     1921 aacatatccc ttcatcaaaa aatcgagtct ttctttcagc gctttaattt tctcttccag
     1981 tttgcccttt ttcttccctg tttcgatttc tttttctctt aatcttcctg ccgcctgttc
     2041 tttttcttca atccggcttg taagttcttt cgttttagcc tgcagttctg ctgccaaagt
     2101 tccgtttttc agttctttga tttcttcttt gagccggtct tggcggcggt aatgatcggc
     2161 cagttcgtct gccgcacccg caagacgcat caaatgttcc ggttcctgac cgaaccgttc
     2221 aaattgccgg gcttctttca acgactcttc cgtctgacga tctgcgatca gcgaactttg
     2281 aagatctata atttcctcct gcagctctct tagctttgcc ttcgatactt ttgtaccgat
     2341 acatgcattt ttcgtataat ccttcctgcg cagatggcgg aacatatatc cgctgtacga
     2401 ataacaatcc ggtgtgactc cgtcttttac cgtacttaac tcttccaccg tctcacattt
     2461 ttgtattctt ccaagataac gtttcaagca ccggtcaaca tatccgcttt ctgcgtgtac
     2521 cgcttcatat agcgagtttt cgtccgcttc cggagaatcc ttttgaaccg cctcactatt
     2581 gatcagatca acttcttcaa actgctgcat cctgcgaaat attttcgctg catcaagagc
     2641 gtatgccggt tctgtaatca tacttttttt aatcctgcca agccgtcctt cgatcgcatt
     2701 tttccatttt tcatccgtaa tatcaaacaa atccgcgaaa atatgaactt cgattttcct
     2761 tccgtacctg tcgctcaacg cacgctgcag ttcctgtctc gcctttttca attccaacgg
     2821 atacggtttt ctgtcgtttt ggagatcttc gacagcatct tgtttttctt tgatgtcttt
     2881 ttccgtttca tggatcgaaa tacgaagttc cgacaattca tttgtaatat tatcaaggac
     2941 caagtcaaga tgtgtcctca gttctttaca taacttttcc gtaagcgctc cgcctcgtat
     3001 ttcctgaata agctgaagag ctctgttgct cacataatca gtgacaacat cctcttcttc
     3061 ccatcgtttc aatcctttca cgatctgccg ccactgtgcc gaattatccg ccagaagagc
     3121 aattctgtat tctaaatctt ttagttcctg ttctttcttt ccataatcgc tgttatttaa
     3181 ttctgcctgt acccgggctc tttcatcccg tatcgtctgc agttcgtcct gaagtatctt
     3241 ctgttctttt ttcgcctcgt ccgcttcttt ttcaacggat tccaactcac tttcccgtcc
     3301 tttgagtcga attatatttt cctcgatatc aatgcatttg agcactgttt ccgttcgtac
     3361 tttctccgca cgcacattcg tcagttctct gtcatatact tggatcttat ccagcatttc
     3421 gatccgtttt tccagatctt ccacccgttc tttgatctct ctgtacgcgc caagctgttc
     3481 actgatcgtc gccaccgtat cttctttcgt tttcggaaac atataatctc ttataaactg
     3541 acctgttcca ctcgtcattt tcagagcaat ggcgcttttt tccatcgtga tcatacgacc
     3601 ttgttctacc tgaccgaaga tttccccgta cagcgtataa agataagctt ctttcgacgg
     3661 atacacttta tttacttcac cccggcctct gttatcggcg cttctgcttc tttcttccgt
     3721 aagttttcta atctgtgcga tcgaataagg tacgttattt tccaaatatt catcctttgg
     3781 aattcttccc gaatgactga aataagtata ttttttcaga tccgtatccc cttttgccac
     3841 ctcgaacgcc gccccgacac aagtcacaat atgagtggcg gtatcttcaa tctcaagaac
     3901 aatctgtgaa caaaaatcaa cgtctcccct gtttgccgtt ccgtcttttt gcgctcctct
     3961 aagatagctt aaaacgcttc tcttgttttt agaatcatct gccgctttat tcagaaaatt
     4021 agcagacaca ctcccataca gaacaacctg tatcgcatcc ataaccgtcg atttccccga
     4081 tccgctcttt ccgctgaaaa gattcacata ctcgtggaat tccaacattt tatggctgat
     4141 gccgccccag ttattgatca gcattcttgt aaaaattttc ttcggctgtt tcatatccgt
     4201 ctgcctcctc tctatattct tcaatcaaat cattcatatc tttcgccgtc acatacagat
     4261 tgatcgtact gtaaatatag atcggcgtat cgtcttctac gtccctcacc gctcccggca
     4321 cgtcgatgat ctgatgaagc cgcatcagat aaagagaagc tttccattca tctgccttca
     4381 attttcttac gatcaggttc gtattctttc cgtattcgcg catttctttc agcgttgtca
     4441 caggagcgtt taacccttct cccataattt tatctttata gatcagtttt atgataagaa
     4501 ggatcaccgt ttctgtcata ttcagttttt cccttgcagc tccgtcgcct gtcaaacaga
     4561 aaatatgctc ttgtgcatca tgcgtaagct cacatccaag taccgaaaga tagtcttcta
     4621 taaaatgtct gtgccggaca cagatttcat actgtacatt atctttcggc acaagtgttg
     4681 ccggatcata tttcacctgc aaaatacatg tctggcgaaa caacgcctgt atcgtctttt
     4741 ttatctgttc cccttctgtg atagaaatac tttccaaata ctcaaacatg acttagtcct
     4801 ctcttatttc cagcgttgaa aaacgcattc cctgttcggt ttcttcttcc tctcctacgg
     4861 tgatctcccc actgccaaac gcattttccg tcgcttcctg ccatataaaa aacaattttt
     4921 ccaaatcttc agccgaccga accgtctgcc gcgtagttct aaatacgttt ccgacactgt
     4981 ttctttcttt gaactcctgc agctctttcc ttgtataaag cggttttaag acaaacgact
     5041 ccaaattttc ttccgcctgt ccgccttccg aaacagtagc cggaacaaac gcttctctcc
     5101 ctttttcttt ccgtttgtac agactttcat ccgtcaacgc tctgtgaggt ctgctaaggc
     5161 ggatacgctc cgccagtttt tccgatattt cttcttttct cctgctttta ttcaacagat
     5221 ctacaagcgc aaccgtacgg tcttcttctg ctccttcctg aagaacgtac cgtattctcg
     5281 cagccgccct ggacgcaaaa atcgtcttct gttcgatcag tttattatat cttctttcga
     5341 tcgcatcaaa ctgcctttcg attcgtatca gcgtatctgc cgcttcctcg cacattgccg
     5401 ccgcccgttc cagccgcagt tcttttattt tctccgcctc atctgttttt ttcggatctt
     5461 taacttttcc cgcgttttct tcgtctttca tcttcttttg acggacctct tccagttccc
     5521 gcaacagttt cagcctgctc atatcatttt cctgtaaatt tctgtcaagc agttctttaa
     5581 cagcttcttt atatcgataa aaactgtccg ttgtcgtcaa gatcgcatat tttcggctgt
     5641 cactgttatt gatctccttt acaaggacgt cctgtattcc ccgaaaatct ttctgacggg
     5701 acagttcatc aaaataccct ctcataccgt cctgcatatt tacaagcatc tgcacaagct
     5761 ttttggaaac acgaagggcg ctttttaaaa tctcattatt tttttcttta tcggaactat
     5821 atgtatacag atggctgtat atcgtcataa cgctttcccg ctcttcgctc tcttgttcat
     5881 caagcagact cctaaacaac tccacataca tctgactata ttcaggaaat gtcacaacgt
     5941 aactgttcaa agcctcgtca tagtcctgtt tcagccatcc ccactcctga aaatgtttca
     6001 gacacacgga cgccatatta gaacgtgtaa gaaactctcc ctcttcatca taattttcct
     6061 catcccacag aagagccgct gccgcgatct tttcattcat aactgccctg cactcttttt
     6121 ccgtcaatcc aagcacggaa taagacagac tcatttcctc atagattgcc gtgagaaaca
     6181 gcatatag
//
LOCUS       DBMDXF010000109         6173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3239_length_6173_cov_1.447464, whole genome shotgun sequence.
ACCESSION   DBMDXF010000109 DBMDXF010000000
VERSION     DBMDXF010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3239_length_6173_cov_1.447464"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="ACZPD4_05515"
     CDS             complement(<1..142)
                     /locus_tag="ACZPD4_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1378558.1"
                     /translation="MNILFFLKPKSEVAYVHDYGTLRQVLETMEYHKYASIPMLNKSG
                     EYV"
     gene            complement(283..1602)
                     /locus_tag="ACZPD4_05520"
     CDS             complement(283..1602)
                     /locus_tag="ACZPD4_05520"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1378559.1"
                     /translation="MKKVVKFGGSSLANADQFKKVGAIITADDTRRYVVPSAPGKRFP
                     KDTKVTDMLYACYESAANGEDFSEQLDNIHARYQEIIDGLNLDFSLDLDFVQIKENFS
                     NKAGADYAASRGECLNGKIMAAYLGFEFVDAAEVVRFNDDGSFNDEVTNTLLSERLQD
                     CKTAVIPGFYGAKKDGTIVTFSRGGSDITGSLVALAVQADLYENWTDVSGFLIADPRI
                     VKNPKSIETITYKELRELSYMGASVLHEDAIFPVRKAGIPINIRNTNAPQDKGTLIVE
                     GTCRQPKYTITGIAGTDGFVAITVEKAMMNSEVGFCRKVLQVFEDNGVSIEHMPSGID
                     TMSIFVNKDVFEEKEQKILADIHKAVNPDHIELESNLALIAVVGRGMKSTRGTAGRIF
                     SALAHAHINVKMIDQGSSELNIIIGVRHDDFKNAIRALYEIFVLTQI"
     gene            complement(1734..2948)
                     /locus_tag="ACZPD4_05525"
     CDS             complement(1734..2948)
                     /locus_tag="ACZPD4_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR1378560.1"
                     /translation="MKRKILIEKSKNKIRTYLIEDDDIVEIHTSINEEQVPPGKLGDI
                     YIGKVQNIVQNIGAAFIEITKGVNCYFDLKDAENAYFTHKSGKKPLCIGDELVVQISR
                     EAVKTKAPTVTSHLSFTGRYAVLTHGNTRIGVSSKIPRALRDEFKDRLSRMKNEQFGI
                     IIRTNAKGVPFQEVEDEIERLKEEYKKLLNTALSRVAFSRLKSAPPTYISDLKNVYME
                     GMEEIVIEGKDLYTEIQEYFLTEFPEKVKLLRLYENPDFPLCKLYSTETALEKALKEF
                     VWLKNGGYLIIQPTEALTVIDVNSGKMAVKSKNSAGAMKVNMEAAQEAAKQIRLRNLS
                     GIIIVDFVNLESKEDTGILLKEFRNFLSKDPIPTTLVDMTMLGLVEITRKKVRKPLYE
                     QENYLQAEIEAL"
     gene            complement(2981..3703)
                     /locus_tag="ACZPD4_05530"
     CDS             complement(2981..3703)
                     /locus_tag="ACZPD4_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR1378561.1"
                     /translation="MKARIKFRKYGALRFIGHLDVMRFFQKVMRRAEIPIAFTGGYSP
                     HMIMSFASPLGIGLTSDGEYFDIELTEAIDSKTAVKRMNEVSIEGIEIISFRKIPDDK
                     KQTGMTILAGADYLVSVRKGALPSDWKEQFSKFMSQPEIQVIKQTKRSERMVDIRALI
                     DSWEIRDEQIYLLLAAGSSENLKPDLVMETFLKYANISDDVRFFYHRLEMYADESGNG
                     ERKLIPLENLGEEICMNSKTDL"
     gene            complement(3687..5552)
                     /locus_tag="ACZPD4_05535"
     CDS             complement(3687..5552)
                     /locus_tag="ACZPD4_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR1378562.1"
                     /translation="MRKLALDDEILLSIEKPARYIGNEVNSIMKNKNEVDIRFAMCFP
                     DVYEIGMSHLGIQILYDMFNRRDDVWCERVYSPWTDLDTIMRKEGIPLFALESQDPIR
                     EFDFLGITIQFEMCYTNILQILELSQIPLHSCDRAENDPIVIGGGPCVYNPEPLAEFF
                     DLFYIGEGETVYDELLDTYKKCRAEGSTRTEFLEQAARIEGIYVPSFYDAEYNEDGTL
                     RSFIPNNICAPASVKKQVVMDVTDTVYPMKPVVPFIKATQDRVVLEIQRGCTRGCRFC
                     QAGMIYRPTRGRNVKRLKEYAKTMLTNTGHEEISLSSLSSSDYSDLKELVMFLIDEFK
                     EKAINISLPSLRIDAFSLDVMSKVQDIRKSSLTFAPEAGSQRMRNVINKGLTEEDIMG
                     GASQAFEGGWTKVKLYFMLGLPTETEEDMKEIAHLCEKVARKYYEIPKDQRNGKCQIT
                     ASSSFFVPKPFTPFQWAPMCTAEEYTRRAHLVNDEFKAQLNRKSLKYNWHDANVTVLE
                     GIMARGDRRIGKVIEEAYRLGCLFDSWTESFRDDLWEKAFLNTGIEKEFYTLRTRDKN
                     ELFPWDFIDTGVSRKFLRKEWERAMNAEVTPNCREHCSGCGASKFGGGVCYEGKN"
     gene            complement(5897..>6173)
                     /locus_tag="ACZPD4_05540"
     CDS             complement(5897..>6173)
                     /locus_tag="ACZPD4_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378563.1"
                     /translation="RQTKQLYGERETVPPIHPRYGNLCKEQDEGGEEAGGSFYMRLVI
                     CILCFIFFVYMDQNHAEVANVNSTKIINEIERDVELEAVKEVWENFE"
BASE COUNT         1694 a         1388 c         1016 g         2075 t
ORIGIN      
        1 cgacatattc gcccgattta ttcagcattg ggatagatgc atatttatga tactccattg
       61 tctcaagaac ctgtcttaaa gtgccatagt cgtgaacata agccacttcg ctttttggtt
      121 ttaaaaagaa taagatattc attgtatatt tccctttcca cacataaacg catcaaaatg
      181 atgcgtttat tgagtataga tcttatgcgg ttttttaaca aatttttctt gatatattct
      241 tcctataatg gtaatccaat aaaaacaaac ggatatagat ttttatatct gggtaaggac
      301 aaaaatttca tataacgcac ggatcgcatt tttaaaatca tcatgtctga ctccgataat
      361 gatattcaat tcacttgatc cctgatcgat cattttgaca ttgatatgtg cgtgggcaag
      421 tgcggagaaa atgcggccgg cagttcctct tgttgatttc ataccgcgtc ctactacggc
      481 aataagcgca agattagact ccagttcgat atgatccgga tttaccgctt tatggatatc
      541 cgctaagatt ttctgttctt tttcctcaaa gacatctttg tttacgaaaa tcgacattgt
      601 atcaataccc gatggcatat gttcgataga aactccgtta tcttcaaata cctgcagaac
      661 tttccgacaa aatccgactt ccgaattcat cattgctttt tcgactgtga ttgctacaaa
      721 tccatctgtt cctgcaattc cggtaatcgt atatttcggc tgacgacatg ttccttcaac
      781 aattaatgtt cctttatcct gcggtgcatt tgtgttccgg atattgatcg gaatccccgc
      841 ttttcttaca ggaaagatcg catcctcgtg gagcacgctc gctcccatat atgaaagttc
      901 acgaagttct ttataagtaa ttgtttcgat tgatttcgga ttttttacaa tgcggggatc
      961 cgcgatcaaa aatccggaaa catccgtcca gttttcatac agatccgcct gtactgccaa
     1021 agctacaaga gatcccgtaa tatctgatcc gcctcttgaa aaagttacga tcgttccatc
     1081 tttttttgct ccgtaaaatc ccggaattac agctgtttta caatcctgaa gccgctcgga
     1141 taaaagagta ttcgtaactt catcattgaa agaaccgtca tcattaaaac ggacaacttc
     1201 ggcagcatcc acaaactcaa agccgaggta agcagccatg atctttccgt ttaaacattc
     1261 tcctctggaa gcggcatagt cagctcccgc cttgttactg aaattttctt taatttgtac
     1321 aaaatcaaga tcaagagaaa aatcgagatt caatccatca ataatctcct gatatcttgc
     1381 gtgaatatta tcaagctgtt ctgaaaaatc ttctccgttg gctgcacttt cataacaggc
     1441 atagagcata tctgttactt ttgtatcttt tggaaatcgt tttcccggcg cagacggaac
     1501 aacataccgt cttgtatcat ctgctgtaat gatcgctccg acctttttga actgatctgc
     1561 atttgccaga gaacttcccc cgaattttac tacttttttc aatcttcttt cctccaaatg
     1621 tagaactcac gcagcgcttt ttcgctgctt ttatttatcg atccatttct cttctcatga
     1681 ataatgagaa aaatttttgt aagcgcaatt ttcagcgcgc tttcggtaaa atattataac
     1741 gcctcgatct ctgcttgtaa ataattttcc tgttcataaa gcggttttct tacttttttt
     1801 cttgtgattt ctacaagtcc gagcatcgtc atgtcaacca aagtagtcgg aatcggatct
     1861 ttcgacaaaa agtttcggaa ttcttttaat aatatcccgg tatcttcttt tgattcaaga
     1921 tttacaaaat ctacaataat gattcctgac agattacgca gacgaatctg cttcgccgct
     1981 tcttgagcag cctccatgtt tactttcatc gctccggcgc tgtttttcga ttttactgcc
     2041 atcttcccgg aatttacgtc aatgactgtc aaagcttccg ttggctggat aataagatat
     2101 ccgccgtttt tcaaccatac aaactctttc aacgcctttt ccaatgcggt ttctgtagaa
     2161 tacaatttgc aaagtggaaa atcaggattc tcataaagtc tgagaagttt tactttttca
     2221 ggaaattctg taagaaagta ttcttgtatt tccgtataca agtcttttcc ttctatgacg
     2281 atttcttcca ttccttccat gtatacgttt tttaagtctg atatatacgt cggaggagcc
     2341 gatttcaaac gtgaaaaagc aactcttgac aatgctgtat ttaacaactt tttatattct
     2401 tctttcagac gttcaatttc gtcttcgacc tcctgaaacg gtacgccttt tgcatttgtc
     2461 cgtataatga tgccgaactg ttcatttttc atacgtgaca ggcggtcttt gaattcatcc
     2521 cgaagcgccc gtggaatttt tgaagatacg ccgatccggg tgtttccatg agttaagacg
     2581 gcatatcttc ccgtgaagct taaatgagaa gttacagtag gcgctttcgt ttttaccgct
     2641 tcccggctga tctgtacgac aagttcatcg ccgatacaaa gtggtttttt cccggattta
     2701 tgggtaaaat atgcgttttc tgcatctttc aaatcaaaat aacagtttac tccttttgta
     2761 atttctataa atgccgcacc gatattttgg acaatattct gcactttccc gatgtaaata
     2821 tcccctagtt tccccggagg tacttgctct tcattgatac tggtatgtat ttcaacaata
     2881 tcatcatctt caataaggta tgtccgtatt ttatttttgc ttttttctat tagtattttt
     2941 cgtttcaaac caaaatcctt cccatgtcat tacagattta ctacaggtct gtctttgaat
     3001 tcatacagat ttcttctccg agattttcaa gaggaatgag cttcctttct ccgttgccgc
     3061 tttcatctgc atacatctca aggcgatgat aaaaaaatcg tacatcatct gaaatatttg
     3121 catattttaa aaatgtttcc atcacaagat ccggttttaa attttccgaa cttcccgctg
     3181 caagaagaag atagatctgt tcatcacgaa tctcccatga atcgataagg gcacgtatgt
     3241 caaccattct ctcgcttctt ttcgtctgtt tgataacttg aatttccggt tgagacataa
     3301 acttggaaaa ctgttctttc cagtcagaag gcagtgctcc ttttctgaca gagacgagat
     3361 aatctgcccc tgcaaggatc gtcattcccg tctgtttttt atcatccgga atttttctga
     3421 aactaattat ttcaattcct tctatactga cttcattcat acgttttaca gccgttttac
     3481 tgtcgatcgc ttcagtcagt tcaatatcaa aatattcccc gtcacttgtg agaccgattc
     3541 caagaggact cgcaaacgac atgatcatat gcggactgta ccctcctgta aaggcgatcg
     3601 gtatttcagc cctgcgcatt actttttgga agaagcgcat tacatcaaga tgtccgataa
     3661 aacgaagcgc tccgtatttt ctgaatttaa ttcttgcctt cataacagac acctcctccg
     3721 aattttgatg cgccgcatcc tgaacagtgt tcccgacaat tcggtgtcac ctcagcattc
     3781 attgcccgtt cccattcctt tctgagaaat tttcgactga ccccggtatc gataaaatcc
     3841 catgggaaaa gttcgttttt atctcttgta cgaagcgtat aaaactcttt ttcgattccc
     3901 gtgttcaaaa aagctttctc ccataaatca tctcgaaaac tttcggtcca tgagtcaaat
     3961 aagcatccga gtctgtaagc ttcttcaatc acttttccga ttcttctgtc acctcttgcc
     4021 ataatacctt ccaaaacagt tacatttgca tcatgccaat tatattttaa gcttttccga
     4081 tttaactgcg ctttaaattc atcattgaca aggtgagcac gtctcgtata ttcttcagcg
     4141 gtacacattg gcgcccactg aaacggagta aacggtttcg gaacaaaaaa tgaggaactt
     4201 gcagtaatct gacattttcc gtttctttga tctttcggga tttcataata tttgcgggca
     4261 accttttcac ataaatgggc gatctccttc atatcttctt cggtttccgt aggaagtccg
     4321 agcatgaaat acagttttac ttttgtccat cctccttcaa aagcctgact tgcgccaccc
     4381 ataatatctt cttcggtaag tcctttattg attacatttc tcattctctg tgatccggct
     4441 tcgggcgcaa atgtaaggct gctttttctg atatcctgaa ccttactcat tacatctaaa
     4501 gaaaaggcgt cgattctcaa agacggtaat gaaatattga ttgctttttc tttgaattca
     4561 tcaatcaaaa acattacaag ttctttcaaa tcagaataat cacttgaact caaggaacta
     4621 agtgagattt cttcatgtcc tgtatttgtc aacatcgttt tggcatactc tttcagacgt
     4681 tttacattac gccctcttgt gggacgataa atcattcccg cctggcaaaa acggcatccc
     4741 cgggtgcatc ccctttgaat ctcaagtaca acgcgatctt gagttgcttt tataaacgga
     4801 acgaccggct tcatcggata taccgtatct gttacatcca taacaacctg ctttttcaca
     4861 cttgcagggg cacatatatt attcggtata aatgatcgaa gtgtaccatc ctcgttgtat
     4921 tcggcatcat aaaatgacgg cacatagatt ccttcgatgc gggccgcctg ttcaagaaac
     4981 tctgttctcg tacttccctc tgcccgacat tttttatatg tatctaaaag ttcatcataa
     5041 acagtttcgc cttctccgat ataaaacaaa tcgaaaaatt ccgcaagagg ttccgggtta
     5101 tagacacatg gacctccacc gatcacaatc ggatcatttt ccgcgcggtc acatgaatgc
     5161 agaggaattt ggctcaactc taaaatctgc aaaatatttg tatagcacat ttcaaattgg
     5221 atcgtaattc ctaaaaagtc aaattctctg atcggatctt gtgactctaa tgcaaaaagc
     5281 ggaattccct ctttacgcat aattgtatca agatctgtcc acggagaata gacgcgttca
     5341 caccaaacat cgtctcttct gttaaacata tcatacaaaa tctgaatgcc gagatgcgac
     5401 atgccgatct cgtaaacatc cggaaaacac atggcgaaac ggatatccac ctcattttta
     5461 tttttcatta tcgagtttac ttcattcccg atataccggg caggtttttc gatacttagt
     5521 aatatttcat catctaaagc aagctttctc atatatttcc ttcctgtttt tgtatatatt
     5581 ttataaattc ttacgaacct attttggact tttcaaaggg aaaaatcgat attttttatg
     5641 ttgtttttcg tatttataca agacgataca acattacaga aacatcttct atgtgattga
     5701 atcatccaat ctacatcatt atatacattt tagggaagaa cttcaagcaa atatctttcg
     5761 tcaatcggaa tcaatcggtg ctggtgttct tagacaaaaa ctccaaagta aaagatatga
     5821 tttcgcagtc agcattgtat acaactcttt tcgttttaat ataaattttc aggtaaatca
     5881 gctgaataaa tagttatcat tcaaaatttt cccatacttc cttcacggct tccagctcca
     5941 catcccgttc aatttcattt atgatttttg tactgtttac attagctact tccgcatgat
     6001 tttgatccat atatacaaag aagatgaaac agagaataca gattacaagc cgcatataaa
     6061 agcttccgcc tgcttcttca ccgccttcgt cttgttcttt acataaattg ccataacgag
     6121 gatgaatcgg aggaacagtt tcacgttcac catagagctg tttcgtttgt cga
//
LOCUS       DBMDXF010000110         6102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3284_length_6102_cov_2.300283, whole genome shotgun sequence.
ACCESSION   DBMDXF010000110 DBMDXF010000000
VERSION     DBMDXF010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3284_length_6102_cov_2.300283"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="ACZPD4_05545"
     CDS             complement(<1..278)
                     /locus_tag="ACZPD4_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378564.1"
                     /translation="MADAVIDLPENDPAIDRAVKIIEENLIKEMPDCHVTSLHENDAG
                     YAPLLQKYEDKGLETLIIDMDFYVEEDAEPGVFTPGTSYEGYSWMFTR"
     gene            complement(476..1108)
                     /locus_tag="ACZPD4_05550"
     CDS             complement(476..1108)
                     /locus_tag="ACZPD4_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR1378565.1"
                     /translation="MPGEQKKVCIDVPYIDQSKLYPTGCESVSTVMLLRFLGIDITVD
                     EFIEKYLEKKSFEERDGQVYGPDPHRYFCGSPYDDESFGCYAPVIREALEKIIGAEYT
                     VTDETGMTTDELVEKYIDQGMPVIYWACINMRDPIVGPQWKLLDTGETFTWISNEHCM
                     LLVGYDEDSYYFNDPYENNGCIRYPKTVVEDRHKAQHMQAVGVVRKTVIQ"
     gene            complement(1098..2627)
                     /locus_tag="ACZPD4_05555"
     CDS             complement(1098..2627)
                     /locus_tag="ACZPD4_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1378566.1"
                     /translation="MDGSMFIGTWWALVPPLLAIVLAFITKEVYSSLFIGVAVGAMLY
                     SGFNPWESFVNFFDIMKNSMNLNILIFDVLLGMIIVLMAKSGGSAAYGRWAGTKIKSK
                     KSALLATTGLGMLIFVDDYFNCLTVGSVMRPVTDRYKVSRAKLAYIIDSTAAPVCIIA
                     PISSWAAAVNSYVPEDAGISGFQLFMNTIPYNLYALLTLTMVIFITVTAFDFGLMKKH
                     ERNAAKGDLFTTGGEEFDQVAEDEINPNGKVIDLVLPVAVLIVSAVGAMIYTGFQGGA
                     TNLITAFSGCDAETSLIFATLVTVFFMLILYLPRKVVTFKGFMDSLAEGFKLMVPAVT
                     ILIFAWSLKGMGDALGLAEFVGGIVGDNASASIFIPAILFVVAIFLSFSTGTSWGTFA
                     ILVPIATALFAGADNLTMMTISVSAVLAGAVCGDHISPISDTTVMSSAGAQSNHINHV
                     STQMQYAAVVATVSFVGYILAGIIRVWWIVLGISLILLIGVLMGMRMFLERKQVKKHA
                     G"
     gene            2843..4357
                     /gene="glpK"
                     /locus_tag="ACZPD4_05560"
     CDS             2843..4357
                     /gene="glpK"
                     /locus_tag="ACZPD4_05560"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR1378567.1"
                     /translation="MSKYIMAMDAGTTSNRCILFNKKGQVCSVAQREFTQYFPHPGWV
                     EHDADEIWACQLGVAVEAMNKIGASAEDIAAIGITNQRETAIVWDRKTGEPVYNAIVW
                     QCRRTSEYCDSLKEKGLTEKFRQKTGLVIDAYFSATKIKWILDNVPGARERAERQELL
                     FGTVETWLIWKMTKGRAHVTDYSNASRTMLFNINTLSWDDEILSELDIPKSMLPQPKP
                     SSCIYGKTDPAFFGGEIPIAGAAGDQQAALFGQTCFQAGEAKNTYGTGCFLLMNTGEK
                     PVFSENGLVTTIAWGLDGKVTYALEGSIFVAGAAIQWLRDEMRLIDSAEDSEYMASKV
                     EDTNGCYVVPAFTGLGAPHWDQYARGTIVGITRGVNKYHIIRATLESIAYQVNDVLEA
                     MKADSGIALSSLNVDGGASANNFLMQVQADIINAPVNRPSFVETTAMGAAYLAGLASG
                     YWKSKEEIANNRSTDKIFLPSVCEELRLSRIKGWNKAVKYSYGWAKNNDSEKSF"
     gene            complement(4375..5319)
                     /locus_tag="ACZPD4_05565"
     CDS             complement(4375..5319)
                     /locus_tag="ACZPD4_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR1378568.1"
                     /translation="MSADFEGNDKDAVVTQQVLDVIDSYGFKKEGAYNLRLNGLAVCH
                     GDSRHIKIVPKEDRQGIDIYISGEAKEEQVHIPVVMTEEGVDIVYNDFYIEDGADVTI
                     IAGCGIHGEGCSETRHDGIHAFHVGKNANVKYIENHYAQGNGSGKKTLNPVTKIYVGE
                     NSVFTLETTQISGVDCTERENYIELADNAKLFVTERLMTDEEQSAVSNMDIELNGTNS
                     SARIVSRSVGKGNSKQVFHPRAIGNNRCHAHVQCDSIIMDQAEISSIPEINAKHVDAQ
                     IVHEAAIGRINDEQLVKLRTFGMSEEEAEAVIIENFLN"
     gene            complement(5309..6055)
                     /locus_tag="ACZPD4_05570"
     CDS             complement(5309..6055)
                     /locus_tag="ACZPD4_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1378569.1"
                     /translation="MLQAEHLSYNITEEGRQSEILTDVSFTVEDGEMLVITGPNGGGK
                     STLAKLLMGINEATGGKIILDGQDITSYTIDKRAQAGMGFAFQQPPRFKGMTVKRLLS
                     LAAGRELDDTTCCELLSSVGLCAKEYINREADATLSGGEMKRIEIATVLAKDHKICVF
                     DEPEAGIDLWSFSMLIKRFEQIHKEKKQSLILISHQERIIRMADRIMVIGDGKVEAVG
                     TADEIVPILFGKKPESCGCRIQKGGELYER"
BASE COUNT         1678 a         1596 c         1129 g         1699 t
ORIGIN      
        1 tcacgtgtaa acatccaact atatccttca tatgaagttc ccggagtgaa cacgcccggt
       61 tcggcatcct cctcaacata aaaatccata tctatgatca gtgtctctaa tcccttgtct
      121 tcatattttt gaaggagcgg agcatatccc gcatcatttt catgaagaga agttacatgg
      181 cagtccggca tttcctttat gaggttttct tcgattattt taactgcgcg gtcgatagcc
      241 ggatcatttt caggcagatc tataacagca tccgccaata ttttttccga agccttttct
      301 tcttttataa aagaaaaata agccgataaa actaaatacg agcaatatag aaatcaaagg
      361 aatcaatcgt tttttattca tatctcaaaa tctccttaga caaaatcttt tcagactctt
      421 ttatctgttg ccgtcaccat attcccaaac cataccgagc tcatccatat accggttact
      481 gaatgaccgt ttttcttact acgccgactg cctgcatgtg ctgtgcttta tggcgatctt
      541 ccacaactgt tttcggatat cggatgcagc cgttattttc atatggatca ttaaaataat
      601 aactatcttc gtcataaccg acaagcaaca tacaatgttc gttggagatc cacgtaaacg
      661 tctctcctgt atctaagagt ttccactgcg gacctacgat cggatcacgc atattgatac
      721 acgcccaata aataaccggc atcccctgat cgatatattt ttcaaccagt tcatcagttg
      781 tcatcccggt ctcgtccgtc acggtatatt cagcgcctat tattttctca agggcttccc
      841 ggatcaccgg agcatagcat ccaaacgact cgtcatcgta cggacttcca caaaaatacc
      901 gatgcggatc aggaccgtaa acctgcccgt ctctttcttc aaaagacttc ttttccaaat
      961 atttttctat gaattcgtca accgttatat caatgcccag aaaccgaaga agcattaccg
     1021 tcgatacact ttcgcatccc gtcgggtaca gcttgctctg atcgatatac ggaacatcaa
     1081 tacatacttt tttctgctca cccggcatgt ttctttacct gctttctctc caagaacatc
     1141 cgcattccca taagaacacc gatcagaagg atcagactga ttccaagtac gatccaccac
     1201 acccgtatga ttcccgcaag aatatagccg acaaaactta ccgtggcgac aactgccgca
     1261 tactgcatct gagtagacac atggttgata tgattgctct gcgctccggc agacgacata
     1321 acagttgtgt cggaaatcgg cgaaatgtga tctccgcaaa ccgctccggc aagaacagcc
     1381 gatacagaaa tcgtcatcat cgtcaggtta tccgctcctg caaaaagagc tgtcgctatc
     1441 ggaacgagaa ttgcaaatgt tccccacgac gttcctgtag aaaatgacag aaagatcgct
     1501 actacaaaca aaatcgccgg aatgaagata cttgccgaag cgttatctcc tacaattccc
     1561 ccgacaaatt cagccagtcc gagagcatct cccatccctt tcaaagacca tgcaaaaata
     1621 aggatcgtaa cagccggaac cattaattta aagccttctg cgagactgtc cataaatcct
     1681 ttaaacgtta cgacttttct cggaagatac aaaatcaaca taaaaaatac tgtaacgagt
     1741 gtcgcaaaga tcaagcttgt ctctgcatcg catcccgaaa aagctgtaat aagatttgtc
     1801 gctccgccct ggaatcctgt atagatcatt gctccaaccg cagatacgat caaaaccgct
     1861 accggaagta caaggtcgat caccttaccg ttcggattga tctcatcttc cgcaacctga
     1921 tcgaattctt ctcctcctgt cgtaaacaga tctcctttcg ccgcgtttct ctcatgcttt
     1981 ttcatcagtc cgaagtcaaa cgccgtcacc gtaatgaaaa taaccatcgt cagtgttaaa
     2041 agcgcataca agttatacgg gatcgtattc ataaaaagct gaaatccgct gatccccgca
     2101 tcttccggca cgtaagaatt taccgcagcc gcccaactgg agatcggcgc gataatacag
     2161 acaggagccg ctgtcgaatc gatgatatac gcaagtttcg ctcttgacac tttatacctg
     2221 tctgttaccg gtctcattac cgatccgaca gtcagacagt taaaataatc gtctacaaaa
     2281 ataagcatac caagacctgt cgtcgccaaa agggcgctct ttttcgactt gatcttcgtt
     2341 cccgcccatc ttccataggc tgcggaacct cctgactttg ccataagaac aatgatcatt
     2401 ccaagcaaaa cgtcaaaaat caaaatattc agattcatac tgtttttcat aatgtcaaaa
     2461 aaattgacaa atgactccca cggattaaat ccgctgtaaa gcattgcacc taccgccacg
     2521 ccgataaaaa gagaactgta cacctctttt gttataaatg cgagcacgat tgcaagcagc
     2581 ggcgggacaa gcgcccacca tgttccgata aacattgatc cgtccatact tcactctcca
     2641 tctctcttaa tcctttttct tttcgtatcc ccgtcctttt catgacggtt tctctacgat
     2701 tataccatac tttcttttat atcaaaagca tttatcgaag agaataatgc tgatataata
     2761 gctaataaca tcaccattat caaaactcgt ctcacatata caaaaacttc tatcaaaatg
     2821 tgatgacaaa ggaggtacta tcatgtcaaa atatatcatg gctatggatg ccggaacgac
     2881 aagcaaccga tgcatccttt tcaataaaaa ggggcaagtg tgcagcgtcg cccaaagaga
     2941 attcacccaa tattttcctc atcccggatg ggtagagcac gatgccgacg aaatatgggc
     3001 ctgtcagctc ggagttgccg tagaagcaat gaacaagatc ggtgcatcgg cagaagatat
     3061 cgcggcgatc ggtattacaa accaaagaga aaccgctatc gtatgggacc gaaaaacagg
     3121 agaaccggtc tataacgcca tcgtctggca atgccgccgc acttccgaat actgcgattc
     3181 tttgaaagaa aaaggtctta cggaaaaatt tcgtcaaaaa acgggtcttg taatcgacgc
     3241 ttatttttcc gccacaaaga tcaaatggat actggacaat gtccccggag ccagagaacg
     3301 tgcagaacgt caggaacttc ttttcggcac agtggagaca tggctcatct ggaaaatgac
     3361 aaaaggacgg gcacatgtaa cggattactc caatgcttcc cgtacgatgc tgtttaacat
     3421 caacaccctt tcctgggacg acgagatcct gtccgaactt gacattccga aaagtatgct
     3481 tcctcagcca aaaccgtcaa gctgtatcta cggaaagacc gatcccgcat ttttcggcgg
     3541 agagattcct atcgcgggag ctgccggcga tcagcaggcc gcccttttcg gtcagacttg
     3601 ttttcaggcc ggagaagcca aaaacactta cggaacgggc tgctttttat taatgaatac
     3661 aggcgaaaaa cctgtatttt ccgaaaacgg acttgtgaca acgattgcct ggggattgga
     3721 cggaaaggtg acttatgcgc ttgaaggctc tattttcgta gcaggagccg ccatacaatg
     3781 gctcagagat gaaatgcgcc ttatagattc agccgaagat tcggaatata tggcgtcaaa
     3841 agtagaagat acgaacggat gttacgtcgt tcctgctttc acaggtcttg gcgctcctca
     3901 ctgggatcag tatgcacggg gaacgatcgt aggtatcacc cgcggagtaa acaaatatca
     3961 catcatccgc gccactcttg aatcgatcgc ttatcaggta aacgacgtgc tggaggcgat
     4021 gaaagccgat tccggcattg cgctttcttc actcaatgta gacggaggtg caagcgcgaa
     4081 taattttctc atgcaggtac aggcagatat tataaacgcc cctgtcaatc gtccttcttt
     4141 tgtagagacg accgccatgg gcgctgctta tcttgccgga cttgcttccg gttattggaa
     4201 aagcaaagaa gaaatcgcaa ataaccgctc aaccgataaa atcttccttc cttctgtctg
     4261 tgaagaactt cggcttagcc gcatcaaagg atggaataaa gctgtaaaat attcctacgg
     4321 atgggcgaaa aacaacgatt ccgaaaaatc attttaaaaa accgatgccg aaaatcaatt
     4381 caggaaattt tcaatgatga cagcttccgc ttcttcctcg ctcataccga acgtgcgaag
     4441 cttcacaagc tgttcatcat tgattctgcc gattgccgct tcatgaacga tctgcgcatc
     4501 tacatgtttt gcattgatct ccggaataga gcttatctct gcctgatcca taataatcga
     4561 atcacactgc acatgtgcat gacaccggtt attgccgatc gctctcggat gaaagacttg
     4621 ttttgaattt cctttcccga ccgatctgga aacaattctt gccgagctgt ttgttccgtt
     4681 taactcaatg tccatattgg aaactgccga ctgttcttca tctgtcatca gcctttctgt
     4741 cacaaacagt ttcgcgttgt ccgccagctc aatataattt tctctctccg tacagtctac
     4801 gccgctgatc tgcgttgtct caagcgtaaa tactgaattc tctccgacat agatctttgt
     4861 caccggattg agcgttttct tacccgagcc gtttccctga gcataatgat tttctatata
     4921 tttcacattc gcatttttac ctacatgaaa tgcatggatt ccgtcatgac gggtttccga
     4981 acacccttcc ccgtggattc cgcatcctgc gataatcgtt acatctgctc cgtcttctat
     5041 atagaaatca ttgtatacaa tatccacgcc ttcttcagtc attactacag gaatgtgaac
     5101 ctgctcttct tttgcttcac cgcttatata aatatcgatt ccctgtctgt cctctttcgg
     5161 aacaatctta atatgacggc tgtccccatg acaaaccgcc aaaccgttca gtctgaggtt
     5221 atatgcccct tcctttttaa atccataaga atcgatcaca tcaagcactt gctgtgtcac
     5281 aacagcgtct ttgtcatttc cttcaaaatc agcgctcata aagttcgcct cctttttgta
     5341 ttctgcatcc gcatgattcc ggtttcttcc cgaacaagat cggaacgatc tcatccgccg
     5401 tgccgaccgc ttctactttt ccgtctccga taaccatgat cctgtccgcc atgcgtataa
     5461 tacgctcttg atgtgagatc aagatcagac tctgcttctt ttctttgtga atctgttcaa
     5521 accgcttgat caacatcgaa aaactccaca ggtcgatccc tgcttccggt tcatcaaata
     5581 cacatatctt atgatctttt gccagaactg tcgcaatttc aatcctcttc atctctccgc
     5641 ccgaaagcgt cgcgtcagct tctctgttaa tatattcttt cgcgcaaaga ccgacactgc
     5701 ttaacaactc acagcatgtc gtatcatcta attctctgcc tgccgcaaga gaaagcagcc
     5761 tttttactgt catcccctta aagcgcggcg gctgctgaaa tgcaaacccc attcctgcct
     5821 gcgctctctt atcgatcgta tacgatgtaa tatcctgccc gtccagtatg atcttaccgc
     5881 ctgtcgcctc attgatcccc ataagaagtt tcgcaagcgt cgatttacct cccccgttag
     5941 gacctgtaat gacaagcatc tccccgtctt ccaccgtaaa acttacatct gttaatatct
     6001 ctgattgtct tccttcttct gttatattgt acgataaatg ttctgcctgt aacattccgg
     6061 aaattcactt cctttttctt ttttatttct ctttttattt tt
//
LOCUS       DBMDXF010000111         6018 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3334_length_6018_cov_1.489606, whole genome shotgun sequence.
ACCESSION   DBMDXF010000111 DBMDXF010000000
VERSION     DBMDXF010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3334_length_6018_cov_1.489606"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="ACZPD4_05575"
     CDS             complement(<1..932)
                     /locus_tag="ACZPD4_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1378570.1"
                     /translation="MRDYVITVNSTVDVPKLWLEERHVPVVALKYTIDGETYTDMEGL
                     TAKEFFEKLREGKMSVTSQVNPEEAMEALEPFVKEGKDILHLGFSSGLSGTLNSMRLA
                     GEMLEEKYPEAKVIVVDTLCACMGEALLLHKALQKKAEGKTIEEVAKWTEENKLHICH
                     NVTVDDLNHLHRGGRVSKTTAVLGTLVQIKPIIHMDNEGKLQVIGKERGRKKSLNKIV
                     DMAVKQSEGWENDLIMITHGDCIEDAEYVAQLVREKMGVENILINNIGTVIGSHTGPG
                     VVAVFSEQGQKECREMLAFYKENARIMAEGLRRKG"
     gene            complement(1101..2468)
                     /locus_tag="ACZPD4_05580"
     CDS             complement(1101..2468)
                     /locus_tag="ACZPD4_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1378571.1"
                     /translation="MVEKNTKKLNSSFEENIRYMNEILPVKESFDIIRREIIIGGKAS
                     VFYYIDGFIKDEAMLKIMDSFLSVSEQDMPKDAEMFIQKHVPYVEVEILEDFDQVIRN
                     VLSGPACLFIDGYKECIALDCRTYPARGVDEPDKDKSLRGSRDGFVETIVFNTALMRR
                     RIRDPHLVMEMTEAGQSSRTDIAICYMKDRVDKELLQNLKKRIETLELNDLRMNQQSL
                     AEALFKRKWFNPFPKFKYTERPDTAAACLFEGKVVILVDNSPSAMILPTSIFDMIEEA
                     NDYYFPTVTGMYLKITRTLITVATVFFTPLYLLFMQNIEWLPEVFRFVEVQDTVNIPL
                     VFQFIILELSIDGLRLAAMNTPTMLSTPLSVIAGIVMGEFSVQSGWFNSEVMLYMAFV
                     AVANYTQPNFELGYALKFMRLLLLVLTACFNLYGFIIGCILVFCFLVFNKTLSGRNYM
                     NVKLN"
     gene            complement(2599..3273)
                     /locus_tag="ACZPD4_05585"
     CDS             complement(2599..3273)
                     /locus_tag="ACZPD4_05585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1378572.1"
                     /translation="MKIIKRLLSFIFLCVIIAGGVLGYKGYEEYKKALSEESVKEMAA
                     RIEEQPNYTTIDELPQTYIDAVLSVEDKRFYDHFGVDPIAVGRAFFNDIKAGAYVEGG
                     STITQQLAKNQYFTQDKKIVRKVAEMFMAFKIESELDKDTIFELYVNSIFFGNGYYCV
                     ADASNGYFGKVPSEMNFDECTLLAGVPNAPTNYNPTASPELARQRQKQVIEKMKKAGY
                     LEESVD"
     gene            complement(3457..3993)
                     /locus_tag="ACZPD4_05590"
     CDS             complement(3457..3993)
                     /locus_tag="ACZPD4_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase/dUTPase family protein"
                     /protein_id="HIR1378573.1"
                     /translation="MKRIARFEKVSFGQFSDGWDDTFQAIEKGENKDQSKETLKEIYD
                     RIKLPRRATAGSAGYDFFAPVDIILNPGETIKIPTGIRVWMEPEWVLKCYPRSGLGFK
                     FRLQLNNTVGIIDSDYYNSDNEGHIFAKLTNDSNEEKTVQIKAGEGFMQGIFVEYGIT
                     VDDAATDVRNGGFGSTTK"
     gene            complement(3990..5189)
                     /locus_tag="ACZPD4_05595"
     CDS             complement(3990..5189)
                     /locus_tag="ACZPD4_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1378574.1"
                     /translation="MDSKHFSTLINDFKPGIFGALNEKKEELLKEGRKVYNLSVGTPD
                     FEPPLHVMKAMEEACKDPNNYKYSLADMPELLEAVQYRYEKRFGVHVEKDEIMSVYGS
                     QEAMAHIGMVFCDPDNVILVPNPGYPMFEMSGILAKANVEYYTIEEENGYLPDLDSIP
                     EEVLKKTKYMIVSYPLNPVCVCAPDEFYEKLIAFAKKNEIVILHDNAYSDITYGDKPG
                     KSFLSFEGAKDVGVEFYSLSKSYNLTGARISFVVGNKEIISKFRLLRSQIDYGIFYPV
                     QYAAIAALIGPDDFIEEQRRQYASRNKTLCGGLRRIGWDVRDSQGTMFVWAKIPEGYA
                     SSVDFCMKLMERTGVIVTPGSAFGSNGEGYVRMALVVNESVIEEIVDVLDASGIFKKN
                     SIKESDI"
BASE COUNT         1786 a         1467 c          851 g         1914 t
ORIGIN      
        1 ccttttctgc gcagtccttc ggccatgatc ctggcatttt ctttatagaa agccagcatt
       61 tcgcggcatt ccttttgccc ctgctcactg aacacagcta ctacacccgg acctgtatga
      121 cttcctatca ctgttccgat attgttgatc aaaatatttt caacacccat cttctcacgt
      181 acaagttgcg ccacatattc cgcatcttcg atacagtctc cgtgagtaat cataatcaaa
      241 tcattctccc agccttctga ctgttttact gccatatcta caattttatt caaagatttt
      301 ttacgtcctc tttcttttcc gatcacctgt aattttcctt cattatccat atgaatgatc
      361 ggtttgatct ggacaagcgt tccgagaact gccgttgttt tagatacacg acctcctcta
      421 tgaagatgat tcagatcatc caccgtcaca ttatggcaaa tatgaagttt attttcttct
      481 gtccactttg caacttcttc tatcgtcttg ccttccgcct ttttctgcag cgctttgtgt
      541 aatagtagag cctctcccat gcaggcacaa agcgtatcga ctacgataac ttttgcctca
      601 ggatattttt cttccaacat ctcccctgca agacgcatac tgtttaaagt tccgcttaaa
      661 ccggacgaaa acccaagatg taaaatatcc tttccttctt tcacaaatgg ttctaatgct
      721 tccatagcct cttccggatt cacttgcgaa gtaacagaca tcttcccctc gcggagtttc
      781 tcaaaaaact cttttgcagt caatccttcc atatctgtat aggtttctcc gtcaatcgta
      841 tattttaatg caacaaccgg aacatgtctt tcttccaacc ataatttcgg cacatcaacc
      901 gtgctgttta ctgtaatcac ataatctctc atttttgcat gcctcctgta caaatatcag
      961 aacacagata atttctgtat tcttatattt acatataata ccattttttt agacattacg
     1021 caaaattttt taacaaaaat taaaatttgt aataacagtt cttccataca caaaaacttg
     1081 tctgccgcaa aaaccgaaaa tcaatttaat tttacattca tataatttcg tcctgataat
     1141 gtcttattaa atacaaggaa acaaaacacc aaaatacacc caattataaa tccatacaga
     1201 ttgaaacatg ccgtcaaaac caaaagaaga agacgcataa attttagcgc gtatcctaat
     1261 tcaaaattag gctgtgtata atttgccaca gctacaaacg ccatgtacag catcacctca
     1321 ctgttgaacc aaccggactg taccgaaaat tcgcccatca caatacccgc aatcacgcta
     1381 agaggcgtac tcaacattgt cggggtattc attgccgcca gacgaagtcc gtcaatcgac
     1441 aattctaaaa taataaactg aaagacaagc ggaatattta ctgtatcctg cacctctaca
     1501 aaacgaaaca cttcaggcag ccattcaata ttctgcataa ataataaata taatggtgta
     1561 aaaaataccg ttgcaactgt gatcagcgtt cttgtaatct ttaaatacat tcctgttact
     1621 gtcggaaaat aataatcatt tgcttcctct atcatatcaa aaatcgaagt aggaaggatc
     1681 attgccgacg gagaattatc tacaagaatt acaactttcc cttcaaaaag acatgccgct
     1741 gccgtatccg gacgctctgt atatttaaat ttaggaaaag gattaaacca ttttcgttta
     1801 aaaagagctt ctgcaaggct ttgctgattc atccgcagat catttaattc caatgtttca
     1861 atccttttct ttaagttttg taaaagttcc ttatccactc tgtctttcat gtaacaaatc
     1921 gcaatatctg tacgcgaaga ctgccctgcc tccgtcatct ccatgacaag atgcggatcc
     1981 cgaatccttc tccgcatcag tgctgtgtta aatacgattg tttctacaaa tccgtctctt
     2041 gatccgcgaa gcgatttatc cttatcgggt tcatcgactc ctctcgccgg atatgtcctg
     2101 cagtcaagag caatacattc cttatatcca tcaataaaca ggcaggcagg accggaaagg
     2161 acgttgcgga tcacctgatc aaaatcttcc aagatttcta cttccacata aggaacatgt
     2221 ttctgaataa acatttctgc atctttcggc atatcctgtt cggatacaga aagaaaggag
     2281 tccatgatct ttaacattgc ttcatctttt ataaatccat caatgtaata gaacacagac
     2341 gcctttccgc ctattatgat ctcccttctg ataatatcaa aactttcctt aaccggaaga
     2401 atctcattca tatatcttat attttcttca aatgaactgt ttaacttttt cgtatttttt
     2461 tctaccatag aaacttcccc ttttttctgc cggataccga cagtacattt tctatggtta
     2521 gaataccctt tttttatttt tttattcgat cggcttttta atatattatt tctttttacg
     2581 ctataacggt ttaccatgct aatcaacgga ttcttcaaga tatccggctt ttttcatttt
     2641 ctcgatgacc tgtttctgtc tctgccgtgc aagttcggga ctggctgtag gattatagtt
     2701 tgtcggagca ttcggcactc cggcaagaag ggtacattcg tcaaaattca tctccgacgg
     2761 aactttacca aaatagccgt ttgaggcatc cgccacgcaa taatatccat ttccaaagaa
     2821 aatagaattt acatacaact caaaaatcgt atctttgtca agctcggact cgatcttaaa
     2881 cgccataaac atttccgcca ctttccgtac gatcttctta tcctgcgtaa aatactgatt
     2941 ttttgcaagc tgctgggtga tcgtgctgcc gccttctaca taagctcccg ctttgatatc
     3001 attaaaaaag gctcttccaa ccgcaatcgg atcgaccccg aaatggtcat aaaaccgttt
     3061 atcttccact gacaaaacag catcaatata agtctgcggc aattcatcta tcgtcgtata
     3121 attcggctgc tcttcaattc ttgcagccat ctctttcaca ctttcttctg acagagcctt
     3181 tttatactcc tcatagcctt tatatccaag aactccccct gcaatgatta cacataaaaa
     3241 aataaaagaa agcagtcttt ttattatttt catctgaaac gtatcctcct ctttcgtaaa
     3301 agcatttccc cgctgcatat tcaatatata aaatctatcc gctttcttcc atccttttag
     3361 cttacgtttc accgggagac tcttacattt ttgtaagccg aaaaaacatc cccctgatcc
     3421 acttttattc tctgccctaa atgtcagagc cgtgttttat tttgtcgtgc ttccgaatcc
     3481 tccatttctc acatcagttg cagcatcgtc aacggtaatc ccatattcca caaaaatccc
     3541 ctgcataaag ccctctccgg ctttgatctg aactgttttt tcttcattac tgtcgtttgt
     3601 cagttttgca aaaatatggc cttcattatc cgaattataa taatcactgt caataattcc
     3661 aaccgtatta ttcagttgca gacgaaactt aaacccaagt ccgcttctcg gataacattt
     3721 taacacccat tccggttcca tccaaaccct gatgcctgtc ggaattttaa tcgtttctcc
     3781 cggattcagt ataatgtcaa ccggagcaaa aaagtcatat cccgcacttc ctgccgttgc
     3841 acgtcgaggc agcttgatcc gatcgtaaat ttccttaagt gtttctttgc tttgatcttt
     3901 attttctccc ttttctattg cctgaaatgt atcatcccag ccatctgaaa attgtccgaa
     3961 acttaccttt tcaaatcttg ctattctttt catatatctg actccttaat gctgttcttt
     4021 ttgaaaattc cagacgcatc caaaacatct acaatttcct caatgacact ttcatttact
     4081 acaagcgcca ttctgacata cccttctcca ttgcttccaa aagcgcttcc cggggtaacg
     4141 ataacgcctg ttcgctccat aagcttcata caaaaatcca cagacgatgc atacccttcc
     4201 ggtatcttcg cccaaacaaa catcgttccc tgactgtctc tgacatccca gccgattctc
     4261 cgaagtcctc cacacaatgt tttattgcga gacgcatact gccgcctctg ctcctcaata
     4321 aaatcgtccg gtccgatcaa tgccgcgatt gcagcatact gtaccggata aaaaatacca
     4381 taatcaatct gtgaccgtaa aagacgaaat ttactgataa tttctttatt acctacaaca
     4441 aaagaaattc ttgctccggt cagattataa gacttagaaa gagaatagaa ttctactccg
     4501 acgtcttttg ccccttcaaa cgaaaggaat gattttcccg gcttgtctcc atatgtaata
     4561 tctgaatacg cattatcatg cagaattaca atctcattct ttttcgcaaa agcaatcaat
     4621 ttctcataaa actcatccgg agcacataca cagaccggat tgagcggata tgaaacaatc
     4681 atgtacttcg ttttctttaa aacttcctcg ggaatactat ccagatccgg caggtatccg
     4741 ttctcttcct caatcgtata atattctacg ttcgctttcg cgaggattcc gctcatctca
     4801 aacatcggat atcccggatt cggaacaaga atcacattgt ccggatcaca aaataccata
     4861 ccgatatgag ccattgcttc ctgagaaccg taaacagaca taatctcatc cttttctaca
     4921 tgcaccccaa aacgtttctc atatcggtac tgcaccgctt ccaataattc cggcatatct
     4981 gccaatgaat atttataatt attcggatct ttgcacgcct cctccatcgc tttcattaca
     5041 tgcaatggag gctcaaaatc cggcgttcct acagaaagat tgtatacttt tcttccttct
     5101 ttcaaaagct cttctttctt ctcatttaac gccccgaaaa tcccaggctt aaaatcattg
     5161 atcaaagttg aaaaatgctt gctgtccaat acaaacgccc cctaacatat gataaaacat
     5221 tctttaattt tcaaatcgaa ttgtttctat tctatcacag ttgtcatttt atgtaaatga
     5281 taggcgcaat cccgcttacc gtatccacat tcctgcatga aatgtattga aaaagagatt
     5341 ttaactgact atctaaaaaa agaaacgaga tggtcaatat gttgttagaa gactattaac
     5401 taatattctt tttgatagag ctttttattt actgatactc aggtatttcc aatgttaaat
     5461 aagatgtttt gtaaatccat atttccgata aaatctttcc ttctgaaaac atccccttac
     5521 ctctcctcaa atttttcaaa caagagttga atcagacact cataaaacac actttcaacc
     5581 tcttcaatca tctctgcctt tccattctct ctgtaactta ttcaactctg tttttcccca
     5641 cacacattta atgcgaacat cattgctaca aatactgcta aaaatcagat atgctgccaa
     5701 aagaactaaa agaaatttta catacagaat ttgtcaagta ttttgaaaga aaaagccaaa
     5761 aaccacgcag aaagagacag agaaaaaata aactttgcgc ggattatatt tacaaagata
     5821 cataactaca aaataaaacg gatgatcttt aagaaagact tagagtataa ttattattgg
     5881 caaaatcatg acaacccgtc caacgggcaa tgtcgtcaac ttaagatata tcaaaaatat
     5941 aaaataaaaa tgtctaaaag cacttgataa aatgcgaaaa aaatgttgcc ttgcgttgat
     6001 tactttttca atgcgagg
//
LOCUS       DBMDXF010000112         5929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3391_length_5929_cov_1.889671, whole genome shotgun sequence.
ACCESSION   DBMDXF010000112 DBMDXF010000000
VERSION     DBMDXF010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_5929_cov_1.889671"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..5839)
                     /locus_tag="ACZPD4_05600"
     CDS             complement(<1..5839)
                     /locus_tag="ACZPD4_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HIR1378575.1"
                     /translation="MENVKKLMRKAMGIVLSVVLTLGCILGSGTIAWAEGETADAAAK
                     IKVACVGDSLTEGYTSTGANSGKKGPNAYPARLQSLLGSGYEVKNFGETGAFLMEGTS
                     NPYKSGTEYEQSKAYNADIVIIMLGTNDSKNWNEENYKTQMTAFYNEYKTENNKVIFA
                     TSPKCYQTTGNDITQEKVEKVYQAQKALIKENAAWDFIDMYEKTEGKENLYNTDKVHF
                     TDAGYLYIAECMYEKITGKKAPIEEKVKIPHTQQSGETNYFTFAEGKWEAGDAQHTWS
                     KKVDPAHPEATYYEVKFTGHKIDIYSGKNRMMGKVKYLIDGEEKAEGDLYNATNINST
                     FITTISDLEEKEHTLKAVATGERNASGTGSDILIDAAEVYVYTYREPEEAKLHGTITD
                     NNLQYTQDKLTEVKAANKTEDTLNAWKNDTVTSEISLASIDSSYTDVTATASDFTGNG
                     NTIPKANVDLKFIKSVQAYTGMPGYGDPDREVPKGSRQEANEVLYQDAGTAMNIGTNK
                     LQNIWVSVKVPAGTAAGSYTGTVTVTAKELAEPLTFTYTLKVADAELPDATKFKDGFD
                     MELWQNPYRIAEYYDVTPFSEEHFEILKSHMGLYKNAGGHAITTTIVEEAWAGQTYGE
                     GTVKFPSMVKWTKKADGKWSFDYTDFDKWVTFNKDVMKIGDKIVCYSIAPWTNSVVYY
                     DEATKTTVKKRLTPGSEEWTTIWTAFLNDLADHLYEKGWFNDAYIGIDERGFNKAAFD
                     LIDSIKRGIPTPLKLKSAGAMDGFVEKKDLAMRVDDLNVGSIAVKAHPQQFEEIRQAR
                     EDAGLRTTIYTCTGHKPGNFSLSAPGESYWTMMYSYSVGGEGYLRWAYDSWVENPLED
                     TTHNAFEAGDCFLVFPDDSEAKVTKSSTRFEKMAEGVRDVNKLIKMKSDVPSMAAKVD
                     ALMDSVKPTYASSAYYLTDAGKQELASDMNTIKTKIAELTEEYVALKKAGVTEVDSVS
                     INGEKEVELQQGGTTQLEVTVLPENVLNNTVTWSTDNPAVAVVKDGAVTAKGSGRAVI
                     TVTSNQNEEKKDSVTIVVKEAEVEEGAQVSYYSFDDVKGQKLEDEWGKRDGTLDASCT
                     TEAGKSGKALNVTQDGKGAVITQTAGDLNDKDWTISYWVKTTSEFNKEISVLEDSTKA
                     FSFSLKMAADRVSGFRVGNNGGDVLSYQYDFEKDKWYHITWVQDKDQGLAMYVNGTRV
                     GDINAWTKNNPIKTPADVIGGTGFTGLIDEVKVYNRVLNESEILSAMKVKGLNIADTN
                     PTVNIGETYQITTNLITDAEDKTITYVSNKPEIASVNEDGLVTANKKGKAVITVTGGG
                     YTEKVTVNCKKVLYANSRIPQYELADKYLKDIEKAPNTPRQYLGQPDMVQTKTGRLIT
                     AYPTGHGHGPIIMQISDDEGETWTEKKDIPASFATCQETPTLYTLDMGNGKERIMLIS
                     ACPNWDLQRGGWDTAYSDDDGETWTEYKNHWPSFDGAEKKHTIVAMASLVQLKDEQGN
                     YIQKWMGVFHDYGYVNFKSYLTFDEDGNEQWSEPERYLADYRSIESKYGICEVGMFRS
                     PDGKRIMALARSDKKPNLSVMFYSDDEGKTWSKPEEMQGSLAGERHKAVYDPISGRLL
                     ITFREIVYKDGKLDNNWMAGDWVAWVGTYEDLLEQNEGEYRILIEEDWAMNAKSGDTG
                     YAGILVLDDGTFIMDSYGHFDEEFSKNAFESGNYNVRTDLCYIKQAKFKLGEIENENG
                     LIDRSALEAKINEVKDTSAEGYTDTSYAAFAKALTDAQTVFADYSAQQIQIDEALKVL
                     TSAFEGLEERPVEPEKPTVEKVEIKANPAKTEYKEGEKFDPAGLVLTVKYNKGEDKEV
                     AYSDATKADFAFNPSLDTALTEGVSKVDVTYAGKTVEIGIEVKADTPVEPEKPTVDKI
                     AVKKVPAKTTYKVGEKFDPAGLVLTVKYNKGEDKEVAYSDATKADF"
BASE COUNT         1383 a         1490 c          956 g         2100 t
ORIGIN      
        1 cgaagtctgc ctttgttgca tcactgtaag ctacttcttt gtcctcgcct ttgttatatt
       61 tcactgtaag cacaagtcct gccgggtcga atttctcccc tactttgtaa gttgtcttag
      121 ctggcacttt tttaacggca attttgtcta ccgtcggttt ttccggctct accggtgtgt
      181 cagctttcac ttcaataccg atttctaccg tcttgcccgc atatgttaca tcaactttgc
      241 tcacaccttc tgtaagcgct gtatcaagtg acggattaaa tgcgaagtct gcctttgttg
      301 catcactgta agctacttct ttgtcctcgc ctttgttata tttcactgta agcacaagtc
      361 ctgccgggtc gaatttctcc ccttctttgt actctgtctt agcaggattt gctttaattt
      421 caactttttc taccgtcggt ttctccggtt ctaccggtct ttcttccaaa ccttcaaatg
      481 ctgaagttaa tactttcagc gcttcatcaa tctgaatctg ctgtgcggaa taatctgcaa
      541 atactgtctg tgcatccgtc agcgcttttg caaatgccgc ataagaagtg tctgtgtacc
      601 cttctgccga cgtatctttt acttcattga tttttgcttc cagggcgctt ctgtcaatca
      661 gaccgttttc attctcgatt tcaccaagtt tgaattttgc ctgtttgata taacataaat
      721 ctgttcttac attataatta cctgattcaa atgcattctt agaaaactct tcgtcaaagt
      781 gtccgtaaga atccatgata aatgttccat catccagaac taaaattccc gcatatcccg
      841 tatcaccgga ttttgcattc attgcccagt cttcttcgat cagtatacgg tattcacctt
      901 cgttttgctc taacaggtct tcatatgttc caacccatgc aacccagtcg cctgccatcc
      961 agttgttatc aagcttaccg tctttgtata cgatctcacg gaatgtgatc aaaagacgtc
     1021 cgctgatcgg atcatataca gctttatgtc tttcccctgc aagcgaacct tgcatttctt
     1081 ccggtttact ccatgttttt ccttcatcat cagaatagaa catcacggat aagtttggtt
     1141 ttttatcact tcttgccagc gccatgatcc gttttccatc cggtgagcgg aacattccga
     1201 cttcacagat tccatatttg ctttcgatac ttctataatc agctaaatat ctttccggct
     1261 cgctccactg ttcatttccg tcttcatcaa atgtcagata gctcttgaag ttgacataac
     1321 cataatcatg gaaaacaccc atccatttct gaatgtaatt tccctgttca tcttttaact
     1381 gcacaagact tgccattgct acgattgtat gttttttctc tgctccgtca aaagaaggcc
     1441 agtgattctt atattctgtc caagtttctc cgtcatcatc agagtaggca gtatcccatc
     1501 cgcctctttg taaatcccag ttcggacatg ctgaaatcag cataattctt tcttttccat
     1561 tacccatatc caatgtatat aaggtaggtg tttcctgaca agttgcaaac gatgccggaa
     1621 tatctttctt ctccgtccaa gtctcgcctt catcatcact aatctgcata ataatcggtc
     1681 catgaccgtg tcctgtcgga taagcagtaa tcagtctgcc tgtctttgtc tgaaccatgt
     1741 ccggctgtcc aagatactgt ctaggtgtat tcggagcttt ttcaatatct ttcagatatt
     1801 tatccgccaa ttcatactgt ggaatccttg aatttgcata caatactttc ttacagttta
     1861 ctgtaacttt ttccgtatat ccgccgcctg ttacagtaat gaccgctttt cctttcttgt
     1921 tcgccgttac taaaccgtcc tcatttacac ttgcaatttc cggcttgttt gagacatatg
     1981 taatcgtctt atcttcggcg tctgttatca agtttgttgt aatctgatac gtttctccga
     2041 tatttaccgt tggattcgta tccgcaatgt ttaatccttt tactttcatt gcggaaagta
     2101 tttcactttc attcaataca cggttataaa ctttcacttc atcgatcaga cctgtaaatc
     2161 cagtaccgcc gatcacatcc gcaggtgttt taataggatt attttttgtc catgcattga
     2221 tatcaccgac tcttgtaccg tttacataca tggcaagacc ctgatcttta tcctgcaccc
     2281 atgtaatatg ataccacttg tctttctcga aatcatactg atacgacaag acgtctccgc
     2341 cattatttcc tacacggaat ccggatactc tgtctgccgc catcttcaat gagaaggaaa
     2401 atgcttttgt ggaatcttcc aatacagaaa tctctttatt aaattctgac gtagtcttta
     2461 cccaatacga aatcgtccag tctttgtcat tcagatcgcc tgccgtctgc gtaatcactg
     2521 caccttttcc gtcttgtgtt acgttcagcg cttttcctga tttgccggct tcggttgtgc
     2581 aggaagcatc aagagtaccg tcacgttttc cccattcatc ctctaacttc tgtcctttca
     2641 catcgtcaaa tgaatagtaa gaaacctgcg ctccttcttc aacctcagct tcttttacaa
     2701 cgatcgtaac agaatctttc ttttcttcat tttggttaga tgtcaccgta ataacagcgc
     2761 ggcctgatcc cttcgcagtt accgctccgt ctttcacaac tgcaactgcc ggattatcgg
     2821 tcgaccatgt gactgtgtta ttcaatacat tttccggaag gactgtaact tcaagctgag
     2881 ttgtacctcc ctgctgtaat tcaacctctt tctctccgtt gatcgatact gagtcaacct
     2941 cggtcactcc cgctttcttt aatgcgacat attcttctgt cagctcggca atttttgttt
     3001 tgatcgtatt catatccgac gcaagttctt gttttcccgc atctgtcaga taatacgcgc
     3061 ttgatgcata agtcggtttt acagaatcca tcaaagcatc cacttttgcc gccatgctcg
     3121 gaacatctga tttcatttta ataagtttat ttacatcgcg tacaccttct gccatttttt
     3181 caaaacgtgt acttgatttt gtaacttttg cttcagaatc atccgggaat acaaggaaac
     3241 aatccccggc ttcaaatgca ttgtgagtcg tgtcttccaa tggattctca acccatgagt
     3301 catatgccca gcgaagatat ccttctccgc ctacggaata agaatacatc attgtccagt
     3361 agctttctcc cggcgcactt aatgaaaagt tacccggttt atgacctgta catgtataaa
     3421 tagttgtacg aagtcccgca tcttcacgag cctgtcttat ttcttcaaac tgctgaggat
     3481 gggcctttac cgcgatagaa cctacattca ggtcgtctac gcgcattgca agatcttttt
     3541 tctcaacaaa tccatccatc gctcccgcac tctttaattt caaaggtgtc ggaatgccgc
     3601 gtttaattga atcgatcaaa tcaaacgccg ccttattaaa tcctctttca tcgataccga
     3661 tataagcgtc gttaaaccag cctttttcat ataaatggtc tgcaagatca ttcagaaatg
     3721 ccgtccatat cgttgtccat tcttctgaac ccggagtcaa tcgttttttt actgttgtct
     3781 ttgtcgcttc atcataataa acaactgagt ttgtccaagg cgcaatactg taacatacga
     3841 tcttgtcccc gattttcata acgtctttgt taaacgttac ccatttatca aaatccgtat
     3901 aatcaaaact ccattttcca tccgctttct ttgtccattt aaccatagaa gggaatttaa
     3961 cagttccttc cccgtatgtc tgacctgccc acgcctcttc tacgattgta gttgtaatgg
     4021 catgtccgcc ggcattttta tataatccca tgtgggattt taaaatttca aaatgctctt
     4081 cagaaaacgg cgttacatca taatattctg cgatacgata cgggttctgc cacaattcca
     4141 tatcaaaacc atctttaaac ttcgtcgcat ccggaagttc tgcatcagct acttttaacg
     4201 tataagtaaa tgtaagtggt tctgctaatt cttttgccgt taccgtaacc gttcctgtat
     4261 aactacctgc cgctgttcct gccggaactt tcacagatac ccagatattc tgaagtttgt
     4321 tcgttccaat attcatcgct gttccggcat cctgataaag cacttcattt gcttcctgtc
     4381 tgcttccttt cggaacttct ctgtcaggat caccgtatcc cggcattcct gtataagcct
     4441 gaactgattt aataaatttt aaatccacat ttgctttcgg aatcgtattg ccgtttccgg
     4501 tgaaatcact tgccgtcgct gttacatctg tataagaaga atctatcgaa gctaaagaaa
     4561 tctcagaagt aaccgtatca ttcttccatg cattcaaagt atcttctgtt ttgtttgctg
     4621 cttttacctc agttaattta tcctgggtat actgaagatt attatctgta attgttccgt
     4681 gaagttttgc ttcttccggt tctctgtaag tatatacata cacctcagcg gcatcgatca
     4741 gaatgtccga acctgttcct gatgcatttc tttcccctgt agccaccgct tttaatgtgt
     4801 gttctttttc ttctaaatcg gaaatcgttg taatgaatgt agaattgata ttagtagcat
     4861 tataaaggtc tccctctgct ttttcctcac cgtcgatcag atacttcact tttcccatca
     4921 ttctgttttt accggaatag atatcgatct tatgtcctgt aaatttcact tcataatagg
     4981 ttgcttcagg atgcgccgga tctacttttt ttgaccatgt atgctgcgca tctcctgcct
     5041 cccattttcc ttctgcgaat gtgaagtagt ttgtttctcc gctctgctgt gtatgcggaa
     5101 ttttcacctt ttcttcaatc ggagcttttt ttcctgtaat cttttcatac atgcattctg
     5161 ctatgtacag atatcctgca tctgtaaaat gtactttatc tgtattataa agattttctt
     5221 ttccttcggt cttttcatac atatcaatga aatcccatgc ggcattttct ttaatcaatg
     5281 ccttctgagc ctgatagact ttttcgacct tttcctgagt gatatcgttt cccgtcgtct
     5341 gataacattt cggagacgtg gcaaaaataa ctttgttatt ctccgtctta tactcattat
     5401 aaaacgctgt catctgtgtt ttataatttt cttcgttcca gtttttggaa tcattcgttc
     5461 caagcataat aattacaatg tctgcattat aagctttact ttgttcgtat tctgtaccgc
     5521 ttttatatgg attacttgtc ccctccatca aaaacgcacc tgtttcccca aagtttttta
     5581 cttcatatcc gcttccaagt aaactctgca aacgtgccgg atatgcattt ggtcctttct
     5641 ttcctgaatt cgcgcccgtc gaagtgtacc cttccgtcag gctgtctccc acgcatgcca
     5701 ccttgatctt cgcggcggca tctgccgtct caccttctgc ccatgcgatc gtgccgcttc
     5761 ctagaataca ccctaatgta agcacaacac ttaaaacaat tcccattgct tttcgcatta
     5821 gttttttgac attttccaca actatttttc cttcctttct tagcattttg cccacatact
     5881 catacagaac catataccct tttgttcaat gtgtcaacat tatttttac
//
LOCUS       DBMDXF010000113         5852 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3436_length_5852_cov_1.568075, whole genome shotgun sequence.
ACCESSION   DBMDXF010000113 DBMDXF010000000
VERSION     DBMDXF010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_5852_cov_1.568075"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="ACZPD4_05605"
     CDS             <1..1001
                     /locus_tag="ACZPD4_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR1378576.1"
                     /translation="TEHAIYYDKQEPKEFSALELTYLSAYEKRLFPSVCEILRTYYSE
                     RSLITRIRQKSVDLRHIVQTALERNRKKYDLQMRQLKDTENRDKYKVYGELINAYGYN
                     VPEGAKQMEALNYYTNETVTIPLDPTSTPQENAQRFFAKYNKQKRTFEALTQLIRETK
                     DEISYLESIQTSLDIAMTEDDLAAIKEELSETGYVRRKTVRKKIKLKNEPLHYISSDG
                     FHMYVGKNNLQNDALTFDFAAGCDWWFHAKQAPGSHVIVKTNGEELPDRTFEEAGKLA
                     AYYSSMRGSEKVEIDYIEKKHIKKPKGAKPGFVVYYTNYSLVIDSDIRGIQKISGS"
     gene            complement(1113..2405)
                     /locus_tag="ACZPD4_05610"
     CDS             complement(1113..2405)
                     /locus_tag="ACZPD4_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1378577.1"
                     /translation="MRTNELMLYKNMEHGRILKNMTFLMENCGNDYYNREDLRSLLFE
                     CMNEILEISASHGFEGNLWHTYLTFLLVNDENAYSTSCEIVGEIDGSINRIALHDFQI
                     FKELFDYDFQEMEREFSAECMQILFEYKNTSNAGKVFNRRIRDRICNLAEGLADSSDA
                     IDFKRKMTQFYKEFGVGKFGLHKAFRIDHPEGKTMSVEPITNIAHVHLDDLVGYEIAK
                     KKLIDNTEAFVKGKKANNCLLFGDAGTGKSTSIKAVLNQYYDQGLRMIEVYKHQFQDL
                     NDVIAQIKNRNYKFIIYMDDLSFEEFEIEYKYLKAVIEGGLERKPDNVLIYATSNRRH
                     LIRETFRDKADRDEELHTNDTVQEKLSLVARFGVTIYFGSPDKKEFRQIVRTLAQKNK
                     IEMPEEDLLLEANKWELSHGGLSGRTAQQFIDYLMGQN"
     gene            complement(2501..3631)
                     /locus_tag="ACZPD4_05615"
     CDS             complement(2501..3631)
                     /locus_tag="ACZPD4_05615"
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HIR1378578.1"
                     /translation="MTSLGELREALDKIDNEIAELYEKRMEVCRKVGEYKVKSGKKVF
                     DRQREKEKLADVMSKVEGDFNKKGIQELYQQLMSMSRKLQYSQLVEAGALGRLPFIRI
                     ENLDRENARVVFQGTEGAYSQAAMYQFFGRDVNNFHVRTFREAMEAIEEGSADYAVLP
                     IENSTAGTVNEMYDLLDEFENYIVAETILPVVHTLSGLPGAKLSDIKKVYSKTEALMQ
                     TSEFLDDHGDWQKISVVNTAVAAKKVIEEQDISQAAVCSAYAAKVHGLEVLVDEINDD
                     EDNSTRFIVVTNQKIFLKNASKISIRFDSPHQSGSLYGILSHFIYNDLNMTKIASRPI
                     KGRPWEYCFFVDFEGNLEDPAVKNAIRGLREEATNLKILGNY"
     gene            4000..4920
                     /gene="pyrB"
                     /locus_tag="ACZPD4_05620"
     CDS             4000..4920
                     /gene="pyrB"
                     /locus_tag="ACZPD4_05620"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1378579.1"
                     /translation="MRHLMSPLDFSVEELDKLLDLAQDIEAHPAKYAHACAGKKLATL
                     FYEPSTRTRLSHEAAMLNLGGSIMGFSSADSSSAAKGESVSDTIRTISCYADICAMRH
                     PKEGAPMVACQHSSIPVINAGDGGHQHPTQTLTDLLTIRNVKGRLKNMTIGLCGDLKF
                     GRTVHSLINALVRYEGIRFILISPEELRLPDYIRHEVLDRNNIPYEEVVRLEDAMPSL
                     DILYMTRVQKERFFNEEDYVRMKDFYILDKAKMKLAPEDMYVLHPLPRVNEISTEVDN
                     DPRAAYFRQVQYGVYVRMALILTLLEIHVD"
     gene            4942..5385
                     /locus_tag="ACZPD4_05625"
     CDS             4942..5385
                     /locus_tag="ACZPD4_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HIR1378580.1"
                     /translation="MVKNTLNVGSITEGFVLDHIEAGKSMEIYKYLHLDKLDCCVAII
                     KNARSNKMGKKDIMKIECPIDFMDLDILGFIDHNITVNIIENKQIVAKKQLTLPKEIR
                     NVIRCKNPRCITSIEQELDHVFVLTDEENQIYRCKYCEEKHRRQR"
     gene            complement(5537..>5852)
                     /locus_tag="ACZPD4_05630"
     CDS             complement(5537..>5852)
                     /locus_tag="ACZPD4_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1378581.1"
                     /translation="EDLIRLLDMYRQRELKNPAAIIDANHSNSNKQFKEQIRIVSEVL
                     HSRNYNSELKKLIKGVMIESYLEEGCQKIEENRIYGKSITDPCLGWDDTERLIYKIAE
                     EC"
BASE COUNT         1714 a         1346 c         1008 g         1784 t
ORIGIN      
        1 ggactgaaca cgctatctac tatgacaaac aagaaccgaa ggaattttcc gctcttgaac
       61 tgacgtatct ttcagcatat gaaaaacgtc tctttccttc tgtttgcgaa attcttcgta
      121 cttactactc tgagcggagc ctgattacga ggattcgcca gaaatccgtc gatcttagac
      181 acattgtaca aactgctctt gaaaggaatc ggaaaaaata tgatctgcag atgcggcagt
      241 taaaagatac cgaaaacaga gacaaataca aagtttacgg cgaactgatc aacgcttatg
      301 gttacaatgt tcccgaaggc gccaaacaga tggaggcgct gaactattac acaaacgaaa
      361 cggttacgat cccgttagat ccaacaagca ctccgcagga aaacgcacag cgtttttttg
      421 ccaaatacaa caaacaaaaa cgtacttttg aagcgctcac ccagttgatc cgggaaacaa
      481 aagatgaaat ttcctatctc gaatccatac agacctctct cgatattgca atgacagagg
      541 acgatcttgc cgccatcaaa gaagagctgt ctgaaacagg atatgtgcgc cgcaaaaccg
      601 tacgaaagaa aataaaactt aaaaacgaac cgctgcacta tatttcaagc gatggttttc
      661 atatgtatgt agggaaaaat aatcttcaaa acgacgccct tacatttgat tttgccgccg
      721 gatgcgactg gtggtttcat gcaaaacaag ctcccggatc acatgtgatc gtaaaaacaa
      781 acggggaaga acttcccgac cgtacatttg aagaagcggg caaacttgcc gcttattatt
      841 caagtatgcg aggcagcgaa aaagtcgaaa tcgattatat agagaaaaaa catattaaaa
      901 aaccaaaagg cgcaaaaccc ggttttgttg tctattatac aaattactct ctcgtcatcg
      961 acagcgatat aagaggcatc caaaaaatat ccggcagtta gaattagcaa aggagtctct
     1021 attctctctg cccggctttt cactgttaaa acatcacaag gcgtcaaaca tgccgaaaaa
     1081 caccgataca tgtctgacgc cttttatcgt tcttaattct gtcccataag ataatcaata
     1141 aactgctgtg ccgtccttcc cgacaaacct ccatgagaca attcccattt atttgcttcc
     1201 agaagcagat cttcttccgg catttctatt ttatttttct gcgcaagcgt acgaacaatc
     1261 tgacgaaatt ctttcttatc gggcgagcca aagtaaatcg tcacgccaaa ccttgcgaca
     1321 agcgataact tttcctgaac cgtatcattt gtatgaagct cttcatctct gtctgcttta
     1381 tcccgaaacg tctcacggat caggtgacgt ctgttagaag tcgcataaat caaaacatta
     1441 tccggctttc tctcaagacc tccttctatt accgctttca aatacttgta ttcaatctca
     1501 aactcttcaa atgaaagatc atccatgtat ataataaact tataattccg atttttaatc
     1561 tgcgcgatca catcatttaa atcttgaaac tgatgcttgt atacttcgat catccgaagc
     1621 ccctgatcgt aatactgatt taaaacagct ttgatcgacg tagattttcc tgttccggca
     1681 tcgccgaata agagacagtt atttgctttt ttccctttca caaaagcctc agtgttatcg
     1741 atcagcttct tcttcgcaat ctcataacct acaagatcat caagatgtac atgcgcaata
     1801 tttgtgatcg gttctaccga catcgtcttt ccttccggat gatcgatacg aaacgcttta
     1861 tgaaggccga atttaccgac tccgaattct ttgtaaaact gtgtcatctt cctcttgaag
     1921 tctattgcgt ccgacgaatc tgcaagtcct tctgccagat tacagatacg atcccgaata
     1981 cgccggttaa aaaccttccc cgcattgctc gtatttttat attcaaaaag aatctgcata
     2041 cattctgccg aaaattcacg ctccatctcc tgaaaatcat aatcaaacaa ttctttaaat
     2101 atctgaaagt catgaagggc aatcctgtta atgcttccgt caatctctcc tacaatctca
     2161 catgacgtgc tgtaagcatt ttcatcattt acaagaagaa acgtcagata tgtatgccaa
     2221 agattcccct cgaatccatg actggcagaa atctctaata tctcattcat acattcaaaa
     2281 agaagacttc tcaaatcttc cctgttatag taatcatttc cacaattttc catgagaaac
     2341 gtcatgtttt ttaatattct gccatgctcc atatttttat acagcatcag ttcatttgta
     2401 cgcattgttc tctctccttc cgttttttcc tgctttaccg ttttatgtta aacatcctgt
     2461 cttttctccc agaaactact ggattacggc atctgccgtc tcaataattg ccgagtattt
     2521 ttaagtttgt tgcctcttca cgaagtccgc gaattgcatt tttcacagca gggtcttcaa
     2581 ggtttccttc aaaatcaaca aaaaaacaat attcccaagg tctccctttg atcggacgag
     2641 atgcaatctt cgtcatattc agatcattat aaataaaatg tgataaaatt ccatacaaag
     2701 aaccgctctg atgcggcgaa tcaaaacgga tgcttatctt ggaagcattt ttcaaaaata
     2761 ttttttgatt cgttacaacg ataaaacgtg tggaattgtc ctcatcatca ttaatttcat
     2821 cgactaaaac ttccagtccg tggacttttg ccgcataagc actgcatact gcagcttgtg
     2881 aaatatcctg ttcttcaatc actttctttg ccgccaccgc agtattgaca acactgattt
     2941 tctgccaatc accatggtcg tctaaaaact cagacgtctg cataagagct tctgtttttg
     3001 aatatacttt ttttatatcc gaaagcttcg cgcccggaag tcccgacaat gtatgcacaa
     3061 ccggaaggat cgtctctgct acaatatagt tttcaaattc atcaagcagg tcatacatct
     3121 cgttcaccgt accggcagtg gaattctcga tcggaagcac cgcatagtca gccgaacctt
     3181 cctctatcgc ttccatcgct tcccgaaatg tcctcacatg aaaattattt acatctcttc
     3241 cgaaaaactg atacattgca gcctgtgaat aagctccttc tgttccctga aatacaacac
     3301 gggcattttc acgatccaga ttttcgattc tgataaacgg aaggcgtccc agcgctcccg
     3361 cttccacaag ctgactgtac tgaagcttcc tgctcataga catgagctgc tgatacagct
     3421 cctggattcc tttcttattg aaatccccct ctacttttga cattacatct gcaagttttt
     3481 ctttttctct ttgcctgtca aacacctttt ttcctgattt cactttatat tctccgactt
     3541 ttctgcagac ttccatccgt ttttcataaa gctctgctat ttcattatca attttatcaa
     3601 gtgcttctct caattctcca agcgatgtca ttttttctcc tccgatgccc ccaaattttt
     3661 cttttcacaa cagtatagta tatttttctt tcaaaatcta catgtttaca aaattaatga
     3721 caaactaaga taacagttca tccatacagg tgtccgctta tgagtaaaag agtggcgtct
     3781 tcgttttatg atacgatacg ccgccttctt tttcatattt tgtttctgtt tttccggctt
     3841 taaacttttc tttttgttca ttcttctttg ccgtatctct ctattttagc aaatatacat
     3901 ttatctgttt ataaaatttc cgactgtttg ttttctctat acggttgaaa agaaaagggt
     3961 tttattatat tataattaaa gatttttagg aggttttgta tgagacattt aatgagtccg
     4021 ctggatttct cagtggaaga acttgacaaa ctgctggatc ttgctcagga tattgaagca
     4081 catccggcaa aatatgcaca tgcctgtgca ggaaaaaaac ttgcaacact tttttatgaa
     4141 ccgagtacac gcacacggtt aagtcatgag gcagccatgt taaatctggg cggaagtatc
     4201 atgggatttt cttctgccga ttccagttcc gcagcaaaag gagaaagtgt atctgatacg
     4261 atccgcacga tttcctgcta tgcagacatc tgtgctatga gacacccgaa ggaaggcgct
     4321 cctatggtcg cctgtcagca ctcttcaatt cctgttatca acgcaggaga cggcgggcat
     4381 cagcatccga cacagacgct gaccgacctg ctcacaatcc gcaacgtaaa aggacgtctt
     4441 aaaaatatga cgatcggttt atgcggcgac ttaaaattcg gacgtaccgt tcactctttg
     4501 atcaatgctc tcgtcagata tgaaggaatc cggtttatct tgatttcccc cgaagaatta
     4561 aggcttcccg attatatccg ccacgaagta cttgaccgga acaacatccc atatgaagaa
     4621 gttgtccgtc tggaagatgc aatgccctct ctggacattc tctatatgac aagagttcaa
     4681 aaagaacgct ttttcaatga agaagattat gtccgcatga aagatttcta catacttgac
     4741 aaagcaaaaa tgaagcttgc gccggaagat atgtatgttc tccatccgct tccgcgagta
     4801 aatgagattt caaccgaagt tgacaatgat ccgcgcgccg cttattttcg tcaggttcag
     4861 tacggcgtat acgtgcgcat ggcattaatt cttacactgc ttgaaattca tgttgattaa
     4921 gaaaggtaaa aggaacagat catggtaaaa aacacattga atgtcggaag cattacagaa
     4981 ggcttcgtcc ttgatcatat cgaggccgga aaaagtatgg aaatttataa atatctccac
     5041 cttgataaac tggactgctg tgtagccatt atcaaaaatg cccgcagtaa taaaatggga
     5101 aagaaagata ttatgaaaat cgaatgtccg attgatttca tggatcttga tattttaggt
     5161 tttatcgatc acaatatcac cgtcaatatt atagaaaaca aacaaattgt cgctaaaaag
     5221 cagctcacgc ttccaaaaga gatccgaaat gtgatccggt gcaaaaaccc acgctgcatc
     5281 acttcgatcg aacaggaact ggatcatgtc tttgttctga cagatgaaga aaatcagatc
     5341 taccgctgta aatattgcga agaaaaacat cgaagacaac gataataaaa tccggcttta
     5401 taagtcgtaa tatacaaaag gaaatggtga gctgtaagaa caatttttta cactgcatcc
     5461 atttcctttt tataatcact cttattttac acttgttttt tctgctgtat gccaaactga
     5521 atcggcttgc tttttatcag cattcttctg cgatcttata gatcagtctt tccgtatcgt
     5581 cccagccaag gcacggatct gtgatcgact ttccgtaaat gcggttttcc tcgatcttct
     5641 ggcatccttc ttccaaatag ctttcgatca ttaccccttt gatcagtttt tttaattcac
     5701 tgttataatt tctgctgtga agcacttcac tgacaattcg gatttgttct ttaaactgtt
     5761 tatttgaatt tgaatggttt gcgtcaatga tcgccgccgg atttttcaat tccctttgcc
     5821 tgtacatatc caaaagacgg ataagatctt ca
//
LOCUS       DBMDXF010000114         5849 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3438_length_5849_cov_2.476862, whole genome shotgun sequence.
ACCESSION   DBMDXF010000114 DBMDXF010000000
VERSION     DBMDXF010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3438_length_5849_cov_2.476862"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="ACZPD4_05635"
     CDS             <1..1070
                     /locus_tag="ACZPD4_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR1378582.1"
                     /translation="AVFVFQHLIPVDGMIISAIVIVGYTALGGFLAASTTDFIQSIIM
                     SVALIIVFIFGINIAGGIPAVVENAQSLPGYLTMMTTYDPVAGVEQPYPIINIISMIA
                     WGLGYFGMPHILLRFMAIEDEEKLTLSRRVATIWVVISLAVAVLIGVIGLAMSDVGAL
                     KTLTGSDSETIIVQIADLLSKHGILPALLAGTILAGILASTMSTADSQLLAASSAVSS
                     DLFGDRVAKTGDKKKAMNAARFTLLAIAVIAAFIARDPNSSVFGIVSFAWAGFGAVFG
                     PVVLFALFWRRSNWQGALAGMISGGAMVFIWKYLVRPLGGVWNIYELLPAFSVSCLMI
                     VIVSLLTKAPSKEITEEFDRV"
     gene            1194..1364
                     /locus_tag="ACZPD4_05640"
     CDS             1194..1364
                     /locus_tag="ACZPD4_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1378583.1"
                     /translation="MAHVISDECVSCGSCEAECPVGAISEGDGKYEIDADACVDCGAC
                     EAACPTGAISAE"
     gene            1611..2093
                     /locus_tag="ACZPD4_05645"
     CDS             1611..2093
                     /locus_tag="ACZPD4_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1378584.1"
                     /translation="MGRTEMGHRYYTKDDIQLFCCIKKLKNEGMLLKDLKPLIPELKE
                     KKSKLKTPEKEKTQLQTVTSETGDILLPLNTIDQMRALVGDVFREALNENNSILKNDI
                     SSTITTDVIHEMDFILEAKERRQEEHFRKLDMLIRRQQTARKESSKSGAIMKLKSIFT
                     "
     gene            complement(2094..3443)
                     /gene="dnaB"
                     /locus_tag="ACZPD4_05650"
     CDS             complement(2094..3443)
                     /gene="dnaB"
                     /locus_tag="ACZPD4_05650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1378585.1"
                     /translation="MENIEAAMKRIPPHSTEAEQAVLGAMLMNKDAITVTSEILAGQD
                     FYQTAYGILFDAMTDLFKSGRPVDLITLQEYLKTKDVPPEVSSMEFARDLLVGTQTSA
                     NVKYYAEIVKEKSVLRSLIKLNEETANACYLENEPLDELLERTEKRVFELAENGNSQE
                     YVPIKQVVLNALDVIEKASKTKGNVTGIPTGFIDLDYKLSGLQRSDLVLIAARPSMGK
                     TAFVLNIAQHVAFRQNLAVAIFSLEMSKEQLVNRLFSLESHVDAQVLRNGNLTDTDWE
                     KLIEGAGTIGSSRMIIDDTSGISISEMRSKCRKYKLEQGLDLIIIDYLQLMSGSGGRK
                     NESRQQEISEISRSLKGLARELNVPVIALSQLSRAVEQRTDKRPMLSDLRESGAIEQD
                     ADVCMFIYRDDYYNPDTEDKNIAEIIIAKQRNGPIGTVRLAWMPQYTRFGNELRQQQ"
     gene            complement(3453..3902)
                     /gene="rplI"
                     /locus_tag="ACZPD4_05655"
     CDS             complement(3453..3902)
                     /gene="rplI"
                     /locus_tag="ACZPD4_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1378586.1"
                     /translation="MKVILLENVKSLGKKGEIVNVNDGYARNFILPKKLGVEATGKNL
                     NDLKLQKNNEKKVAQENLDAAKELAAELSAGKVELAIKVGEGGRTFGSVSSKEIAVAV
                     KDQMQLDIDKKKIQLKESIKSLGTHIVTVKLHPEVSAELKVVVKEEA"
     gene            complement(3903..>5849)
                     /locus_tag="ACZPD4_05660"
     CDS             complement(3903..>5849)
                     /locus_tag="ACZPD4_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1378587.1"
                     /translation="LMVDRKAAFIMFMFILIYLAIAGGLYFYNRSLILADLIQFSTQY
                     KGIENTLLKELAVPYAIALEDGRILWKNDAFSKLTQSSRKEKYLYRMIPALTTSVFPK
                     DDMEHVEMEVEYCDRHYQAELRRVSLHGFSDKEKLLQIPEEEEFFVAVSMQDVTELNA
                     YIRENEEQRMIAGLIYIDNYDEVMESVEEVRQSLLVALIDRKINKYIGDVDGIVKKME
                     KDKYFIVLRKSAYKKIAEDRFSLLEEVKQVNIGNARSATLSIGLGLNTATYALSYQYA
                     RVAIDLALARGGDQAVIKDCSGITYFGGKKEQTAKNTRVKARVKAEALREFIVTKDRV
                     LVMGHKIADPDSFGACMGIYRAAVSLEKKAHIIVNTVTESVRPLYNEIVESPAYEDDI
                     FLTSDEAMDYITDNTMVIVVDTNKPQMTECPELLRRSRMTAVLDHHRQSSTVIANAVL
                     SYVEPYSSSTCEMVAEVLQYISDSIKIPAVEAECMYAGIMIDTRNFMNRTGVRTFEAA
                     AFLRRCGADITRVRKMFRDDMESYRAKAEAMREAEVYKNQFAIAVCPGDIESPTVLAA
                     QTANELLDISGINASFVLTVYQEKIYMSARSIDEVNVQIIAEKLGGGGHINSAGAQFD
                     HTNMEEAISTLKETIDDMIEKGDI"
BASE COUNT         1510 a         1486 c         1142 g         1711 t
ORIGIN      
        1 ttgccgtatt tgtttttcaa catctgatac cagttgacgg tatgatcata agtgcgatcg
       61 tcatcgtagg gtatacggcg cttggcggat ttcttgccgc ctcaacaaca gacttcattc
      121 agagtattat catgtctgtt gccctgatca tcgtctttat attcggcatc aatattgccg
      181 gaggtatccc tgccgtagtt gagaacgcac agtcgcttcc gggatatttg acgatgatga
      241 ctacatacga tcctgttgca ggtgtcgaac agccttatcc gatcatcaat attatttcca
      301 tgatcgcatg ggggctcggt tacttcggta tgcctcatat cctgcttcgc ttcatggcta
      361 tcgaggacga agaaaaactc acgctctcca gacgcgttgc gacaatctgg gttgtcattt
      421 cccttgcagt tgcggttttg atcggtgtga tcggtctggc aatgagcgat gtaggcgctt
      481 taaaaactct gacaggttcc gattctgaaa cgatcattgt gcagatcgcc gacttgttaa
      541 gtaaacacgg tattcttcct gccttacttg cgggaacgat ccttgccggc attcttgcct
      601 ctacaatgtc cacggcagac tcacagcttt tggctgcatc ctccgccgta tcttccgacc
      661 ttttcggcga cagagttgcc aaaacgggag acaagaaaaa agcaatgaac gcagctcgct
      721 tcaccttgct tgccatcgcg gtaatcgccg cttttattgc ccgtgatccg aacagttcgg
      781 tattcggcat cgtttccttt gcatgggcag gttttggcgc cgtattcggt cctgtcgtat
      841 tatttgccct cttctggaga cgctcaaact ggcagggcgc acttgccgga atgatttcag
      901 gcggagctat ggtattcatc tggaaatacc tcgtacgtcc tctcggcggt gtatggaaca
      961 tttatgagct gctcccggct ttcagcgtat catgccttat gatcgtcatt gtaagcctgc
     1021 tcacaaaagc accttcaaaa gagatcacag aagaattcga ccgggtttag gttcttaatt
     1081 tagaacatac aaatcacttg tatattcaaa ctcattgtat taaaatagtg ataataaaga
     1141 gaactacctt tctttgtttc tctttttaaa ttcaaaaaag gaggatacat actatggcac
     1201 acgttattag tgatgaatgc gtaagctgtg gttcttgcga agctgaatgt cctgtaggcg
     1261 ctatctctga aggcgatggc aaatacgaga tcgacgctga tgcttgtgta gactgcggag
     1321 cttgtgaagc tgcgtgtccg acaggcgcta tctcagctga gtaatataaa tcgcaaagtg
     1381 atcacaaaag agaagcaccg ctcctatgtg gagcggtgtt tttttgtagg aacttgacga
     1441 acgatttttt cgttctgaag tcatggtcta tgctataata acctcaaaag caaaatgagg
     1501 aggttcacat attaatgggt gaagtcagat ttatgatttc cgaagccgca aaataggtca
     1561 atgtcgaatc acatgtatta agatattggg aagaagaact cgacctgacg atcgggcgaa
     1621 cggaaatggg acatcgatac tacaccaaag atgacattca gctcttctgc tgtatcaaaa
     1681 aactcaaaaa cgaaggaatg ctcttaaaag acttaaaacc actcattccc gaattaaaag
     1741 aaaaaaaatc taagttaaaa actcccgaaa aagaaaaaac tcaacttcag acagtcactt
     1801 cagaaacagg agatattctt cttcctctta atacgatcga tcaaatgcgt gctctcgtag
     1861 gcgatgtttt ccgcgaagcg ctgaatgaaa acaactccat attaaaaaat gacatcagca
     1921 gtacgatcac cactgacgtc attcatgaaa tggattttat acttgaggca aaagaacgcc
     1981 gtcaggaaga acatttccgc aaactggaca tgctcatccg cagacagcag accgcgcgaa
     2041 aagaatcctc taaatcaggc gcgatcatga aattaaaaag catatttaca taatcactgc
     2101 tgctgacgca gttcatttcc gaaacgggta tactgcggca tccacgcaag acggacggtt
     2161 ccgatgggac cgttcctctg ttttgcgata atgatctccg caatattctt atcctccgta
     2221 tccggattat aataatcatc acggtaaata aacatacata cgtcggcatc ctgctcgatt
     2281 gctcccgatt cacgaaggtc ggaaagcatc ggtcttttat ccgttctctg ctcaaccgca
     2341 cggctcaact gagacaatgc gatcaccggt acgttcaatt ctctggcaag tcctttcaac
     2401 gatcgtgaaa tctccgaaat ttcctgctgg cggctttcat ttttacgtcc gccgcttccg
     2461 ctcatcaact gcaaatagtc gatgatgatc agatcaagtc cctgctccaa tttgtattta
     2521 cgacattttg agcgcatctc cgaaatactg atacccgacg tatcgtctat gatcatacga
     2581 gagcttccga tcgtgcctgc tccctcgatc agtttctccc aatccgtatc tgtcaggttt
     2641 ccgtttctca gcacctgtgc gtcaacatgc gattccagtg aaaacagacg gtttacaagc
     2701 tgttcctttg acatctcaag actgaagatc gctaccgcaa gattttgccg aaacgctaca
     2761 tgctgcgcaa tattgagcac gaacgccgtt tttcccatag acggacgcgc cgctataaga
     2821 acaaggtctg atctttgcag accggacagt ttgtaatcaa ggtcgatgaa ccctgtcggt
     2881 attccggtga cgtttccttt cgtcttagac gccttttcga tcacatccag cgcgtttaat
     2941 acgacctgct ttatcggaac gtattcctgg gaattcccgt tttccgcaag ttcaaacact
     3001 cgcttctctg ttcgttcaag cagttcatca agaggctcgt tttcaagata acacgcattc
     3061 gccgtctctt cgttaagttt gatcaaactg cgtaaaaccg atttttcttt tacgatttcc
     3121 gcataatatt ttacgttggc agatgtctga gttcctacga gaagatctct tgcaaattcc
     3181 atactgctta cttccggagg cacgtctttt gtttttaaat attcctgaag cgtgatcaaa
     3241 tctaccggac gccccgattt aaacagatct gtcatagcgt cgaataagat cccatatgcc
     3301 gtctgataaa aatcctgtcc ggccaaaatc tccgatgtga ccgtaatcgc atctttattc
     3361 atcagcatcg ccccaagtac agcctgctcg gcttctgtac tatggggcgg tatccgcttc
     3421 atggcagctt ctatattttc catgagaact ccttatgctt cttcttttac aacaactttc
     3481 aactctgccg aaacttcagg atgaagtttt actgtcacga tatgcgtacc aagcgacttg
     3541 atcgactctt tcaactggat ttttttctta tcaatatcta actgcatctg atctttgacc
     3601 gctaccgcga tttctttgct ggaaacagaa ccgaacgttc ttccgccttc gccgactttg
     3661 atcgcaagct cgactttgcc tgccgataat tccgctgcaa gttctttcgc agcgtcaaga
     3721 ttttcctgag ccactttttt ctcgttattt ttctgaagtt tcagatcatt cagatttttt
     3781 cccgttgctt ccacgcccag ttttttcggc aagataaaat tccttgcgta tccgtcgttc
     3841 acattaacga tctccccttt tttcccgaga gattttacat tttccaataa aataactttc
     3901 atctatatat ctcctttctc aatcatatcg tctatcgttt ctttcaatgt gctgatcgcc
     3961 tcttccatat tcgtatggtc aaactgcgct cccgcagaat taatatgacc tccgcctccc
     4021 agcttttcgg caataatctg cacatttacc tcgtcgattg aacgcgcact catataaatc
     4081 ttttcctgat aaaccgtcaa tacaaacgac gcgttgatcc cactgatatc aagaagctcg
     4141 tttgcggtct gcgccgcaag aaccgtcgga ctttcaatat cgcccgggca gactgcgatc
     4201 gcaaactgat tcttataaac ctcagcctcc ctcatcgcct ctgctttcgc tctgtatgat
     4261 tccatatcgt ccctgaacat ctttcttacc cgggtgatat ccgcaccgca gcggcgaagg
     4321 aatgccgccg cttcaaacgt acgcactccc gtcctgttca taaagttcct tgtgtcgatc
     4381 ataattcccg cgtacatgca ctccgcctcg acagccggga ttttgatact gtcagagata
     4441 tattgaagca cttcggctac catttcacat gtagatgaag aatacggttc tacataagat
     4501 aagactgcat ttgcgattac cgtactgctc tgacggtgat gatctaaaac ggcagtcatt
     4561 ctggaacgcc tcaaaagttc gggacattct gtcatctgcg gcttatttgt atcgactacg
     4621 atcaccatcg tattatccgt aatataatcc atagcttcat ctgaagtcag aaagatatca
     4681 tcctcatacg ccggactttc cacaatctca ttataaagcg gccgaaccga ctctgtcacc
     4741 gtattcacga taatatgagc tttcttctca aggctgaccg ccgctctgta aattcccata
     4801 caagcgccga acgaatccgg atccgcaatc ttatgaccca tgacaagaac tctgtctttc
     4861 gtcacgataa actctcttaa agcctctgct tttacacggg cttttacacg ggtgttttta
     4921 gcagtctgtt ctttcttacc gccaaaataa gtgattccgc tgcagtcttt tatcaccgcc
     4981 tggtcgcctc cgcgcgcaag ggcaaggtcg atcgccacac gcgcatactg gtaactaagc
     5041 gcatatgtcg ccgtattcag accaagtccg atacttaacg ttgccgagcg ggcatttccg
     5101 atattaacct gctttacttc ttcaagaagc gaaaaacggt cttccgctat ttttttgtaa
     5161 gcagatttgc gaagaacaat aaaatatttg tccttctcca ttttctttac aataccgtcc
     5221 acatcgccga tatatttatt gatctttcga tcgatcagag caaccagaag cgactgtcgt
     5281 acctcttcaa cgctctccat cacctcgtcg taattatcga tgtagatcag ccctgcgatc
     5341 atccgctgct cttcattttc gcggatatac gcattcaatt ctgttacatc ctgcatggat
     5401 accgctacga aaaactcctc ttcctcggga atctgtaaca atttttcctt gtcgctgaag
     5461 ccgtgaagcg atactctgcg caattctgcc tgatagtgtc tgtcacagta ctccacctcc
     5521 atctcaacat gttccatgtc gtctttagga aaaacacttg tcgtaagagc cggtatcatc
     5581 ctgtacagat atttttcttt tcttgaactt tgcgttaatt ttgaaaacgc atcatttttc
     5641 caaagaatac gtccatcctc aagagcgatc gcataaggaa ccgcaagttc ctttaacagc
     5701 gtattttcaa tccccttata ctgcgtggaa aattgaatca aatccgctaa aataagcgat
     5761 ctgttataaa aatacaatcc tcccgcaatc gccagataaa tcaggataaa cataaacatg
     5821 ataaacgctg ctttccggtc taccattaa
//
LOCUS       DBMDXF010000115         5849 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3439_length_5849_cov_1.527488, whole genome shotgun sequence.
ACCESSION   DBMDXF010000115 DBMDXF010000000
VERSION     DBMDXF010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_5849_cov_1.527488"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            227..430
                     /locus_tag="ACZPD4_05665"
     CDS             227..430
                     /locus_tag="ACZPD4_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIR1378588.1"
                     /translation="MKWFDNTALTLVIIGAVNWLLIGVFRFDLVAFICGNMSWLSRII
                     YTLVGLCGLYLISLFGRIKSMSE"
     gene            complement(594..1646)
                     /locus_tag="ACZPD4_05670"
     CDS             complement(594..1646)
                     /locus_tag="ACZPD4_05670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1378589.1"
                     /translation="MKGNHYSDNRRRRRKPIYVKKRRSRKRTSTVIAVLLICLILIAG
                     AGAVGFFILQEYSKDHIRQYEFASYNKKQYTGELFAEDLCVASEDVSLDGVEADPGFH
                     GEGLFNIADSQIKYGYKLFDKLYPASTTKVMTAYLALKYGNLDDVVTMSATSTNFNWD
                     ESTCGLSAGDSLTLYDLICGLLLQSGNDCAAAIAEHISGSQEAFAELMNTEAKQLGAT
                     GTHFVNPHGLHDENHYTTTYDLYLMFNEALKDQAFLDIISMNSYTAKVTSADGTSYTP
                     TWQATNYYSAGEATPPDGIHVYGGKTGTTDQAGNCVILYEKNTAGEPYISVIMGASDK
                     PLLYEEMNELLDAGMK"
     gene            complement(1685..2080)
                     /locus_tag="ACZPD4_05675"
     CDS             complement(1685..2080)
                     /locus_tag="ACZPD4_05675"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1378590.1"
                     /translation="MNIGLIAHDAKKKLMQNFCIAYRGILSKHSLYATETTGALIESV
                     TNLNIHKFLPGHLGGIQQLSSQIESNQIDLFIFFRDPLTPRSHEPSVSDIVKMCDMYN
                     IPAATNIATAEALILALDRGDLDWREMYK"
     gene            complement(2095..3231)
                     /locus_tag="ACZPD4_05680"
     CDS             complement(2095..3231)
                     /locus_tag="ACZPD4_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1378591.1"
                     /translation="MKFLINHIKQQYHIKDYKFGLIISVVAISVIGIMMVGSAKPDFM
                     TKQIMGVILGFGAMVVVSLIDYKWIMNFYWILYVINLILLIAVKIPGLGHSANGAQRW
                     INLGFMQLQPSDLTKILLIIFFARFFSDRELKISSPKVIIQSVALLIPSLILIVTQPN
                     LSTTICIAALFCALIFLAGLNYKFVGTVLAITIPAVAIFLAVAVQPNQPFLHDYQQDR
                     ILAWLEPEKYADDESYQQLNSVMAIGSGQLSGKGYNNDATTSVKNGNFVSEPQTDFIF
                     AIIGEELGFVGCCGVIFLLLLIVIQCILIGLKAKDTGGRIICGGVAALIGIQSFINIS
                     VATLILPNTGLSLPFVSYGLTSVVCFFMGIGLVLNVGLQPNKYQ"
     gene            complement(3228..3437)
                     /locus_tag="ACZPD4_05685"
     CDS             complement(3228..3437)
                     /locus_tag="ACZPD4_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR1378592.1"
                     /translation="MSVHSVSIAKYRLKNLLAADRMHCTADLTEQMKNDLYHAVSKYT
                     EINPAYFSIDITRSDIHIKYMGEDR"
     gene            complement(3434..4240)
                     /gene="minD"
                     /locus_tag="ACZPD4_05690"
     CDS             complement(3434..4240)
                     /gene="minD"
                     /locus_tag="ACZPD4_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR1378593.1"
                     /translation="MGEVIVITSGKGGVGKTTTTANIGIGLAKLGKKVIVIDTDLGLR
                     NLDVVLGLENRIIYNIVDVINGNCRLKQALIKDTQFPELCLLPSAQTKDKSAVSPEQM
                     KKLIEDIRSDFDFVLLDCPAGIEQGFQNAIAGAEHAVVVTTPEVSAIRDADRIIGLLE
                     ASGIRKTDLLINRLRIDMVKRGDMMSVDDVTEILAVNLLGVIPDDEQVVIATNRGEAV
                     AGEECLAGRSYMDICRRLTGEDVPVNDYSKSEGFFGKIAQFFHRNQEENR"
     gene            complement(4270..4914)
                     /locus_tag="ACZPD4_05695"
     CDS             complement(4270..4914)
                     /locus_tag="ACZPD4_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIR1378594.1"
                     /translation="MNKLVTIRSNRYGLDIEFDSETEFDQLLEVLGQKFKQSGRFFKD
                     AQMALSFSGRELTRAEEDRVLAVIRENTQIEILCIIEPNDKNEMMYRSVVEQSLSDIY
                     KKEGIFYKGTLRKRQILEAEESIVILGDVELGAKVIAKGSVIIIGTLYGSVCAGASGD
                     TSAYVVALSMQPKYLSIGDIVAKRQIIYQESLNIKGPKIAVIDGNRIYLDPLTD"
     gene            complement(4932..>5849)
                     /locus_tag="ACZPD4_05700"
     CDS             complement(4932..>5849)
                     /locus_tag="ACZPD4_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1378595.1"
                     /translation="NTGRANIFYNRATQEKTAPGSTFKMISATAGLEEGYIDAYTTTY
                     CSGSFNTVTPSPKCWIYPGGHGALNVVQSLQHSCNVFYYQLGYNMGIDSNGNYDSDLG
                     TDKLRKYAAMYGLDRKSGVEIPEAAPQISDEYSIQSAIGQGTNNFTVSQLNRYVTAVA
                     NSGTVYDLTLIDKTTDAAGNLIKDYSAEVDSTMDEINSSTWDLIHQGMEQMVASSTTF
                     TGLDFSMAGKTGTAQHNELHADHVLFVGYAPAEQPQLSIAVRITYGYNSGYASEIGRD
                     IAKVYFNPETAGELITGSAANLGEGIAGD"
BASE COUNT         1604 a         1245 c         1130 g         1870 t
ORIGIN      
        1 gattgcaaaa agaaagatac cgagattgaa ttctcttttt gttttatatt tttttatatt
       61 gactgaatta ttttttttgg atgacaattc agtatacacc tcctgctttt ttctggcgtt
      121 gctagtatat catttttttt agtatatttc cactttttat ggacataata aaaaatattg
      181 gtagctgaat tgttacaaat attgagatga taaaggagga aataacatga aatggtttga
      241 caatacagct ctgacactcg ttattatagg cgccgtaaac tggcttctta taggggtgtt
      301 cagatttgat ctggtggcat ttatatgcgg gaatatgagc tggctttcca ggatcattta
      361 tacgctagta ggtttgtgcg gcttatactt gatcagtctt tttggcagaa tcaaatcaat
      421 gtcagaataa aagaaaaaca cctcgtaaaa tgatatgatc gaattattta tcggaaattt
      481 catttttacg gggtgttgta ttttttataa cggcggcaga tcaaagttca gatttgtccg
      541 aaaacttgca tcgtttgcga ttccgaaaga tgtcttttga cactttcggg gatctatttc
      601 attcctgcat caagcagttc gttcatttct tcgtaaagaa gcggtttgtc agaagcgccc
      661 atgataacgg aaatatacgg ttctcctgcg gtgttttttt catataaaat tacacagttt
      721 cctgcctgat cggttgttcc ggtttttcct ccgtatacgt gtatcccgtc aggcggcgtc
      781 gcttctccgg ctgagtaata attcgtcgcc tgccatgtag gagtatacga agttccgtct
      841 gccgaggtaa cttttgccgt atatgagttc atagaaataa tatcgagaaa agcttgatcc
      901 tttaatgctt cattaaacat taaatacaaa tcatatgttg ttgtgtaatg attttcgtca
      961 tgaagtccgt gaggatttac aaagtgagtt cctgtcgctc caagttgttt tgcttctgta
     1021 ttcattaatt cggcaaaagc ttcctggctt cccgatatat gctccgcaat agcggccgca
     1081 cagtcatttc cggattgtaa gagcagaccg catatgagat cgtacaaagt cagggaatct
     1141 ccggcagaaa gaccacaggt agactcatcc caattaaaat tcgtggaagt ggcgctcatc
     1201 gttacaacgt catccaaatt tccatatttt aaagcaagat aagcagtcat aactttcgtt
     1261 gtacttgcag gatataattt gtcaaacagt ttgtatccgt atttgatctg agaatctgct
     1321 atattgaata gtccttcacc gtgaaatccc ggatctgctt caacgccgtc aagagataca
     1381 tcttctgaag caacacagag atcttcagcg aaaagttctc ctgtgtattg ctttttatta
     1441 tatgatgcaa actcatattg ccggatatga tcttttgaat attcctgcaa tatgaaaaat
     1501 ccgacagcgc ctgcgcctgc aattagtata agacagatca gaagaaccgc aattactgta
     1561 gaagttcgtt ttcggctccg tctttttttt acgtaaatag gttttcggcg tctgcgcctg
     1621 ttatctgaat aatgatttcc cttcatttta ttcctaccat ttattttttg atgtattaga
     1681 tgtattattt gtacatttca cgccaatcta agtctcctct gtcaagtgcc agtataagag
     1741 cttcggcagt tgcgatattt gtagctgccg gaatattgta catatcacac atttttacaa
     1801 tatcacttac gctcggctcg tgagagcgtg gagtaagcgg atctctgaaa aagataaaca
     1861 aatcaatttg attgctttca atctgagagg aaagctgttg gattccaccg agatgtcccg
     1921 gaagaaattt atggatatta aggtttgtta cgctctcgat cagtgctcct gtagtttccg
     1981 ttgcatacaa actgtgtttg ctgagtattc cacgataagc gatacaaaaa ttctgcatta
     2041 attttttctt tgcgtcatgc gcgatcaatc cgatattcat agctgtcatt ctccttattg
     2101 atacttatta ggctgtaatc cgacatttaa aacgagtccg attcccataa agaaacatac
     2161 gacggatgta agtccgtagc tgacaaaggg aagcgaaaga cctgtgttcg gaagaatcaa
     2221 agttgccaca ctgatattga tgaaactttg aatgccaatc agtgcggcta cgccgccgca
     2281 gataatgcgt cctcccgtat ctttcgcttt taaacctatt aagatacatt gtattacaat
     2341 taataataac aaaaatatga ctccgcaaca acccacaaat cctaattctt ctccgataat
     2401 tgcaaaaata aaatctgttt gcggttcgga aacaaaattt ccgtttttaa ctgaagttgt
     2461 ggcatcgtta ttgtatcctt ttcccgaaag ctgacccgaa ccgattgcca taacagagtt
     2521 taactgttga tatgattcat catccgcata tttttccggc tctaaccagg caagaatacg
     2581 atcctgctgg tagtcatgca aaaaaggctg attcggctgc accgcaacgg caagaaagat
     2641 cgcaacggca ggaatcgtaa tcgcaagaac tgttccgaca aatttgtaat ttaatcctgc
     2701 cagaaaaatg agagcacaaa aaagcgctgc gatacagatt gtagtggaaa gattcggctg
     2761 tgtcacaata aggatgagcg acggaataag cagcgcaaca gactgaatga ttactttggg
     2821 gctgctgatc ttaagctctc tgtcagaaaa aaatctggca aaaaaaataa taagaagtat
     2881 ttttgtcaag tcggaaggct gaagctgcat aaacccgagg ttgatccatc tttgcgcccc
     2941 gtttgccgaa tgacctaatc ctggaatttt tactgcgatc aaaagaatca aatttataac
     3001 ataaagtatc caataaaaat tcatgatcca tttatagtcg atcaaagaga caacaaccat
     3061 tgctccgaac ccaaggatca cccccatgat ctgttttgtc ataaaatccg gtttagcgct
     3121 tccgaccatc atgataccga tgacagaaat tgcaactact gaaataataa gtccgaattt
     3181 ataatctttt atatgatatt gctgtttaat atgattgatt aaaaatttca acgatcttct
     3241 cccatgtatt ttatatgtat atcgctgcgt gtaatatcga ttgaaaaata tgcgggattg
     3301 atttccgtat attttgaaac cgcatgatat aagtcatttt tcatttgttc cgtcaaatct
     3361 gccgtacagt gcattctgtc tgcggcaagc agattcttca gccggtattt ggcgatcgaa
     3421 actgaatgaa cactcatcgg ttttcctcct gatttctatg aaaaaattgc gctatttttc
     3481 cgaagaaacc ttctgatttt gaatagtcgt tgaccggaac gtcttctcct gtcagtctgc
     3541 ggcagatatc catgtaggat cttccggcca gacattcttc tccggcaacg gcttctcctc
     3601 tgttagttgc aataacgact tgttcatcgt cgggaattac tccgaggaga tttacggcaa
     3661 ggatttcggt gacatcgtct acggacatca tatcgcctct cttgaccata tcgatccgca
     3721 gccgattaat caaaagatct gttttgcgaa tacccgacgc ttccagaagt ccgataatgc
     3781 ggtcggcatc ccgaatggca gaaacttccg gggtcgtgac aacaacagca tgttcagcgc
     3841 ctgctattgc attttggaat ccttgttcga tccccgctgg gcaatccagt aaaacaaaat
     3901 caaaatcact ccgaatatct tcgataagtt ttttcatctg ttccggggaa accgcagact
     3961 tatctttcgt ttgggcagat ggaagcaggc aaagctccgg aaactgagta tcttttatca
     4021 atgcctgttt taaccggcag ttgccgttta tgacatccac gatattataa atgatcctat
     4081 tctccagccc taatacgaca tccagatttc taaggccgag gtctgtgtca atcactataa
     4141 cttttttccc gagttttgct agaccgattc cgatatttgc tgtcgttgtc gttttaccga
     4201 ctccgccttt tcctgatgta atgacaatta cttctcccat gatgcttttc ctcctacata
     4261 tgtcctgtct caatccgtaa ggggatcaag atagatacgg tttccgtcga tcacggcgat
     4321 tttggggcct ttaatattta agctctcctg ataaatgatc tgacgttttg ctacgatatc
     4381 tccgatgctg agatatttag gctgcataga aagtgctacg acatacgccg acgtatcgcc
     4441 ggatgctccg gcacagacag atccatataa cgtaccgatt atgatcacac tgccttttgc
     4501 aattactttt gcaccaagtt ctacatcacc tagtatgaca atactttctt ctgcctctaa
     4561 tatctgcctt tttcttaaag tccccttgta gaaaattcct tcttttttat agatatctga
     4621 aagactttgt tctactacgc ttctgtacat catctcattt ttatcatttg gttcgataat
     4681 acacaaaatt tcaatttgtg tattttctct tatgacggca agaacacgat cttcctctgc
     4741 tcgcgtcagt tcgcgtccgc tgaaactaag cgccatctgt gcatctttaa aaaagcgccc
     4801 ggattgtttg aatttttgtc cgagaacttc aagaagttga tcgaattctg tttcggaatc
     4861 aaattcaata tctaatccgt atcgattact tctgatggtc acaagtttgt tcattgtcat
     4921 tcccccgtct tttagtctcc tgcaattcct tctccgagat tagctgcgct tccggtaatt
     4981 aattcaccgg ctgtttcagg attaaaatag acttttgcaa tatctctgcc gatctctgat
     5041 gcatatcccg aattatagcc ataagtaatg cggactgcaa tcgataattg aggctgttcg
     5101 gccggagcat atcctacaaa taatacatgg tcggcatgca attcgttatg ttgggcagta
     5161 ccggtttttc ccgccataga aaaatcaagg cctgtaaaag tggttgagct tgcaaccatc
     5221 tgttccattc cctggtgaat aagatcccat gtgctggaat tgatttcatc cattgtactg
     5281 tctacttctg cagaataatc tttaataaga tttcccgccg catctgtcgt tttatcgata
     5341 agcgtcagat cataaacagt tccgctgttt gcaactgcgg ttacataacg gttcagttga
     5401 cttaccgtaa agttgttcgt accttgtccg attgcagatt gaatcgaata ttcatcagat
     5461 atctgcggtg cagcttccgg tatttctacg ccggatttcc tgtctaagcc atacatagcc
     5521 gcatattttc tcaacttgtc tgttccaaga tcactgtcat agtttccatt ggaatcgatt
     5581 cccatattgt atcccagttg ataataaaag acgttgcagg aatgctgcaa tgattgaaca
     5641 acattcaacg ctccgtgacc tcccggatag atccaacatt tcggactcgg agtaaccgta
     5701 ttaaatgaac cgctgcaata tgttgttgtg taagcgtcaa tatacccttc ttccagtccg
     5761 gctgtggccg agatcatttt aaaagttgat cccggagctg ttttttcctg tgtggcacgg
     5821 ttatagaaaa tatttgcacg tccggtatt
//
LOCUS       DBMDXF010000116         5831 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3448_length_5831_cov_2.238220, whole genome shotgun sequence.
ACCESSION   DBMDXF010000116 DBMDXF010000000
VERSION     DBMDXF010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3448_length_5831_cov_2.238220"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="ACZPD4_05705"
     CDS             <1..845
                     /locus_tag="ACZPD4_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1378596.1"
                     /translation="FMGNIRHSFNTSNQETTFSQMPYRLLYSSYSKYEQDWESYPHTH
                     YFSELFYVLNGCGSFIVEEESFPIRKQNLIIINPSIRHTEVSDKNSPLEYIVLGVEGL
                     NFLIYNDKEYLNIQCEDFSENLDFYFHTILREMDEQKESYETICQNLLEALIIQLSRH
                     TGSPVEMLPSSKKITRECSYAKRFIDSNFRENITLDTLAELTHLNKYYFAHTFTEIYG
                     IAPMNYLAQKRIFTSQELLTSTDLNQAAIAKQCGFSSSSYFSQCFRKICGMTPTAYRK
                     QFIR"
     gene            complement(856..2208)
                     /locus_tag="ACZPD4_05710"
     CDS             complement(856..2208)
                     /locus_tag="ACZPD4_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378597.1"
                     /translation="MKKRDLTKGNVTAVMLLFAVPMILGNIMQQCYNLADTWIVGRFV
                     GAEALAAVGSAYTLMTFLNSILIGMCMGSGALFSICYGRRALRKLKEYIVSSFVLIFS
                     VTVILTIVSYVCLDPILTLMQIPADIYGLMRSYMGLILAGLIFVFLYNYFAFLLRSSG
                     NSVIPLIFLGVGTALNIGLDFFFVAKLGRGVKGAAEATVLAQAAAGLGIALYALVKEP
                     ELNMKKMKEEGIGIRADLLKEIFSYSASTGIQQSVMNFGILMVQGLVNSFGTSVMAAF
                     AAGVKIDTLAYMPAQEFGNAYSIFISQNYGADKKERIRKGTKSAAVTVIMFCAAASAA
                     VFFGAEFLMGIFIDPQKTQIIQIGTGYLKTEGACYCGIGILFLLYGYFRAVERPKVSL
                     LLTILSLGTRVVLAYACAPLFGVGAIWTAIPIGWAMADAAGIILLRRGERTNRMNEIM
                     "
     gene            complement(2216..2935)
                     /locus_tag="ACZPD4_05715"
     CDS             complement(2216..2935)
                     /locus_tag="ACZPD4_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1378598.1"
                     /translation="MEMIRLEHVTKSFGRYKALDDVSIVVEEGEFLTVIGRSGCGKTT
                     MLRMINGLQKPDSGKVYAAGEDVGEADLIRLRRKIGYVIQNKGLFPHMTVEKNIIYVP
                     VISGQKDKRQNRKLAEELIGLVGLEREMLDRYPEELSGGQQQRVGIARALASRPKLLL
                     MDEPFGALDEITKRAMQNELLALQKKLGMTVVFITHDIREAMKLGDRVLVMEQGKIAQ
                     CDTPENVKKNPADEFVKELIG"
     gene            complement(3018..3953)
                     /locus_tag="ACZPD4_05720"
     CDS             complement(3018..3953)
                     /locus_tag="ACZPD4_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1378599.1"
                     /translation="MRLKKITALFLTGALILSMAGCAGNGSKDKKEGSAGNEAQEPVK
                     IATKPMTEQFILGEMLKKLIEEKAGYEVELTKGIGGGTSNIQPAMEKGEFDLYPEYTS
                     SGWILVLGHKAGEVSDDKMLAKLQEEYEEKFDMTWVGLYGQNNTYAVVVREDTAKKYN
                     LETCSDLAAVSDQLVFGGNPDYIERADGLPGMSAAYGFQFKEIKDIDIGLKYQALRNG
                     DIDVTNGYTTDAQISQDDVRALKDDKSYQVNYFCSTVVRKDALEKYPKLKETLELMDG
                     ILTDQKMAELNYQVEEEGRDEAEVAEEFLKTSGLL"
     gene            complement(4053..4706)
                     /locus_tag="ACZPD4_05725"
     CDS             complement(4053..4706)
                     /locus_tag="ACZPD4_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1378600.1"
                     /translation="MEFLQKVIQLYAERKEWFLDLFAAHVGLALTAAVSAGIIGLLLG
                     IFISEHKKAAGPVLTIANIGYTIPAISLLGMLIPVLGIGDKTAITALTIYGIMPMVRN
                     TYAGITGVGEEVVAAAEGMGSTRFQIMTKIKLPLAAGVIIAGVRNMVVMTISVSAIAS
                     FIGAGGLGVAIYRGITIYDSAMTFCGSFLIALLAIVSDLLLGLLERKVKKKTGIKNR"
     gene            complement(4710..5033)
                     /locus_tag="ACZPD4_05730"
     CDS             complement(4710..5033)
                     /locus_tag="ACZPD4_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HIR1378601.1"
                     /translation="MEEEVVLCAASAYEQKFYLNPQFESLPEAVRQELQIMCVLYTED
                     VGGILLVVYDEEGNLELKVEHEENDFAFDEIGSVLKIKQLQQTKRELFESLETFYRVF
                     YLGEE"
     gene            5336..>5831
                     /locus_tag="ACZPD4_05735"
     CDS             5336..>5831
                     /locus_tag="ACZPD4_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR1378602.1"
                     /translation="MIYIGIDVAKDKHDCFITNSEGEVLFNAFTIPNNADGFHDLFQK
                     ISSLTNDFSNVKVGLEATGHYNYNLLGFLIDKGLPTFVINPLHTNLFRKSLSLRQTKT
                     DKVDAHTIALMMMSGVNLQSYSNTSYHNEELKSLTRYRFDKVQERAKLKTSISRLVNI
                     LFPEL"
BASE COUNT         1564 a         1567 c          990 g         1710 t
ORIGIN      
        1 tatttatggg aaatatacga cattcattca acacttctaa tcaggaaaca acattttcgc
       61 aaatgccgta ccgcctgctc tactccagtt attcaaagta cgaacaagac tgggaaagct
      121 acccgcacac ccattatttt tccgagcttt tttacgtact gaacggatgc ggaagcttca
      181 ttgtagaaga agaatcattt ccgatccgaa aacagaatct gatcatcatc aacccgagca
      241 tccgccacac ggaagtttca gacaaaaatt ctcccttgga atacattgtc ctcggcgtgg
      301 agggactaaa ctttcttatt tataacgaca aagaatattt aaatatacag tgcgaagatt
      361 tctccgaaaa tctcgacttc tactttcata cgatcctccg ggagatggac gaacaaaaag
      421 aaagctacga gacgatctgt caaaatcttc tcgaagctct catcattcaa ctttcacgac
      481 ataccggatc gcccgtagaa atgcttcctt cctcaaaaaa gattacccgc gaatgcagtt
      541 acgcaaagcg cttcatcgat tctaacttca gagaaaacat cactcttgac acacttgcgg
      601 agctgaccca tctgaataaa tattattttg cccatacatt taccgaaatt tacgggatcg
      661 cgcctatgaa ctatcttgct cagaaacgga tcttcacaag tcaggaactt cttacttcaa
      721 cggatctgaa ccaggcagcg attgcaaaac agtgcggctt ctcttcttca agctattttt
      781 cccaatgttt ccgaaaaata tgcggcatga ctcctaccgc ttacagaaaa cagtttatac
      841 gttgacagac gacaattaca taatttcatt catcctgtta gtacgctcgc cgcgtcttaa
      901 caggatgatt cctgccgcat ccgccatcgc ccagccgatc ggtatcgccg tccagatcgc
      961 tccaacgccg aacaacggcg cacatgcata ggcaaggaca acccgtgttc caagtgagag
     1021 gatcgtaaga agaagagaaa ctttcggtct ttccactgcc cggaaatatc cgtagagcaa
     1081 aaacaggatt ccgatcccgc aataacacgc tccctctgtt ttcaaatacc ctgttccgat
     1141 ctgaatgatc tgtgttttct gcggatcaat aaaaatcccc atcaaaaact ctgctccgaa
     1201 aaagactgcc gcagatgccg ccgcgcaaaa cattatgaca gtcacggcag cgctttttgt
     1261 tccttttctg atccgttctt ttttatccgc cccataattc tgcgagataa atatcgaata
     1321 tgcattcccg aattcctgtg ccggcatata tgccaatgta tcaatcttta cccctgcagc
     1381 gaacgccgcc ataaccgatg ttccgaaact gttcacaagt ccctgaacca taagtatccc
     1441 gaaattcatc acagactgct ggattcccgt cgatgcagaa taagaaaaga tctctttcaa
     1501 aagatctgcc cttataccga tcccctcttc tttcattttc ttcatgttaa gttccggttc
     1561 tttcacaaga gcgtataatg cgattccaag tcctgccgct gcctgagcaa gaaccgttgc
     1621 ctcagccgcc cctttcactc cgcgcccgag ttttgcgaca aagaaaaaat ccagtccgat
     1681 attaagcgca gttccgacgc cgagaaaaat cagaggaatc accgaatttc ccgatgaacg
     1741 gagcagaaac gcaaaataat tatagagaaa aacaaagatc aatccggcga ggatcagccc
     1801 catataagag cgcatcaatc cgtaaatatc cgccggaatc tgcatcagcg tgagaatcgg
     1861 atcaaggcac acatatgaaa caatcgtcaa aatcactgtc actgaaaaaa taagcacaaa
     1921 cgatgaaaca atatattctt ttagttttct taacgctctt cggccataac agattgaaaa
     1981 aagcgctccg ctccccatac acatgccgat caggatcgaa tttaaaaacg tcataagcgt
     2041 atatgcgctt ccgacagccg ccagcgcttc cgcgccgaca aacctcccga caatccatgt
     2101 atctgccaga ttgtaacatt gctgcatgat gtttccaagg atcatcggca cggcaaacag
     2161 cagcattaca gccgtcacat ttccttttgt cagatcacgt tttttcatag ttttttcatc
     2221 cgatcagttc tttcacaaat tcatccgccg gatttttctt tacattttcc ggcgtatcgc
     2281 actgcgcgat ctttccctgc tccatcacaa gaacccggtc tcccaatttc atcgcttccc
     2341 tgatatcatg cgtaataaac acgactgtca ttcctaattt tttctgtaat gccagaagtt
     2401 cattttgcat agcccgcttt gtaatttcat ccagcgcccc gaatggctcg tccatcagaa
     2461 gaagtttcgg gcgggacgca agcgcccttg cgatcccgac tctttgctgc tgacctccgg
     2521 agagttcttc gggataacgg tcaagcatct cacgctccag accgaccaga ccgatcaatt
     2581 cttccgccag tttccggttt tgccgtttat ccttctgtcc gcttataaca ggaacgtaaa
     2641 taatgttttt ttcaacagtc atatgaggaa aaagcccttt attttgtatc acatagccga
     2701 tctttcttcg aagccggatc aaatccgcct ctcctacgtc ttcccctgcc gcatatactt
     2761 ttcccgaatc cggcttttgc aatccgttta tcatccgaag catcgtcgtc tttccacatc
     2821 ccgatctgcc gatcaccgtc aaaaattcgc cttcttccac aacaatgctc acgtcatcca
     2881 atgctttata tctgccaaag cttttcgtca catgttccag acgtatcatt tccatacgca
     2941 ttcattcccc tgtacttttt tgtttcttcc gaaagtttct tcaaacggtt ctgttctcac
     3001 ggttttcgga aaatatttta cagtaacccg gacgttttta aaaattcttc cgccacttcc
     3061 gcttcgtcac gtccttcttc ctcaacctga taattcagct ccgccatttt ttgatctgta
     3121 agaattccgt ccatcagctc taacgtctct ttgagcttcg gatatttttc aagcgcatct
     3181 ttgcggacaa ccgttgaaca aaaataattt acctgataag acttatcgtc ttttaacgct
     3241 ctcacatcat cctgactgat ctgagcgtct gtcgtatacc cgttcgtcac atcaatatca
     3301 ccgtttctaa gcgcctgata tttcaaaccg atatcaatat ctttgatctc tttaaactga
     3361 aatccatacg ccgcagacat ccccggaaga ccgtccgctc tctcgatata atccgggttt
     3421 cctccgaaaa caagctgatc cgaaaccgcc gccagatccg agcatgtctc aagattgtat
     3481 ttctttgccg tatcttctct tacgacaacg gcatacgtat tgttctgacc atacagccct
     3541 acccacgtca tatcgaattt ttcttcgtat tcttcctgaa gttttgcaag catcttatca
     3601 tcgcttactt cacctgcctt atgtccgagc acaagaatcc atccgctgga tgtatattcc
     3661 ggatagagat caaactctcc cttttccatt gccggctgga tattgctcgt tcctccgccg
     3721 ataccttttg taagttctac ttcataacct gccttctcct cgataagctt tttcaacatt
     3781 tctccgagga taaactgctc tgtcatcggc tttgtggcga tcttaaccgg ttcttgagct
     3841 tcgtttcccg cacttccttc tttcttatct ttgcttccgt ttcccgcgca tccggccatc
     3901 gaaagtataa gcgcgccggt cagaaaaagc gccgtgatct tctttaatct catcttttgc
     3961 ctccttattc atagtgtcct tttatgattt tctgtttata tttttctatt tatatttttc
     4021 tatttatgtt tttttattta tgttttcctg ttttatctgt tttttatgcc tgttttcttt
     4081 ttcactttcc gttccagcaa tccgagcaaa agatcgctta cgatcgccag aagcgcgatc
     4141 agaaaactac cgcaaaacgt cattgccgaa tcatagatcg taatacctct gtaaatcgct
     4201 actccaagac ctcccgctcc tataaacgac gcgatcgccg acacagagat tgtcataacg
     4261 accatatttc ttacgcctgc tatgatgact cccgctgcca gtggaagttt gatttttgtc
     4321 atgatctgga agcgcgtgct ccccattcct tccgccgccg cgactacttc ttccccgacg
     4381 ccggtaattc ccgcatacgt atttcgcacc atcggcatga tgccgtaaat cgtaagagcg
     4441 gttatcgccg tcttatctcc gatccccaaa accgggatca gcattcccag aagtgagatc
     4501 gccggaatcg tataaccgat atttgcgatc gtcagaacgg gacctgccgc ttttttatgt
     4561 tccgaaataa aaatccctaa aagaagtcct atgatccccg cgctcacagc cgccgttaaa
     4621 gccagcccca catgtgccgc aaacaaatcc agaaaccatt cttttctctc agcatacagt
     4681 tggatgacct tttgtaaaaa ttccacaccc tactcctctc cgagataaaa caccctgtaa
     4741 aatgtttcaa gcgattcaaa caattctctt ttcgtctgct gaagctgttt gatcttcagc
     4801 acacttccta tctcatcaaa agcaaaatca ttttcttcgt gctctacttt taactcgaga
     4861 tttccttctt catcatatac gacaaggaga atgcctccca catcttccgt atacaatacg
     4921 cacatgatct gcagttcctg acgcactgct tccggcagcg actcaaactg aggattcaaa
     4981 taaaatttct gttcatatgc gcttgccgca caaagcacaa cctcttcttc catacattac
     5041 ctctctcgta ttataaagta gtagtattta tagaccctct ccaaggtctg tatgctattt
     5101 ccatcataac cattctttac cttttctaca ggtatggtta tgatatataa gatgctgtgg
     5161 actggtgttt cctctctacc actttgatgg tttcagaaag tgtccagcca cagtctcttt
     5221 gcttgattgt taatcagtta gataccgcat gacggtttat ttagaacgag ttcacaactg
     5281 cgaagagcaa ctgtggttct aaaacagtgt caaatatatt caaaggagat ttgccatgat
     5341 ttatattggt attgatgttg caaaagacaa acacgattgt tttatcacaa actcagaagg
     5401 agaagtgctt tttaacgcat ttactattcc aaacaatgcg gatggtttcc atgacctctt
     5461 tcaaaagatt tcttccttaa caaatgattt ttctaacgta aaagtaggac tggaagctac
     5521 cggacactac aattacaatc tgttaggttt tctcattgat aaaggtctcc ccacctttgt
     5581 catcaaccct ttgcatacca atctgtttcg taaaagtctt agccttagac agacaaaaac
     5641 cgataaagtg gatgcccata cgattgctct tatgatgatg tctggagtaa acttacagtc
     5701 ctactcaaac acatcttatc acaacgaaga gcttaagtca ttaaccagat acaggtttga
     5761 taaagtacaa gaacgagcaa aactgaaaac ttctatctca cgccttgtta acatcctttt
     5821 ccctgaactg g
//
LOCUS       DBMDXF010000117         5824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3457_length_5824_cov_1.713961, whole genome shotgun sequence.
ACCESSION   DBMDXF010000117 DBMDXF010000000
VERSION     DBMDXF010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3457_length_5824_cov_1.713961"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            238..861
                     /locus_tag="ACZPD4_05740"
     CDS             238..861
                     /locus_tag="ACZPD4_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HIR1378603.1"
                     /translation="MKLEVAIIERKEQLDDLIPFRVENALWGTEKFPPMYGYIGFIPQ
                     DAFYLKLICEEKNPLRVYTEINDPIYRDSVMEAYLMFEPVSSRANEENYLNFAVNANG
                     VLHAEYGSRRMYRSYFTEAERVGFECHAEVHENDWTAELKVPVKILEKIYGPLNLGEG
                     SYFTCNFYKISESKEAEHYASYSPILSSVPSFHVPEFFADAKIVRQK"
     gene            946..1344
                     /locus_tag="ACZPD4_05745"
     CDS             946..1344
                     /locus_tag="ACZPD4_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378604.1"
                     /translation="MILENNEEKRSTLWRTIVAISSILIVIVAVFFVTRLFVGNPLVG
                     TWVSEDSGEVIKIESNGKVTLTDEDKKSFETVQSEVDTKTKVFTIHTEDTQAEGLLSG
                     TYDYSIEKDTLTLTEREYGDQLVFVKREKK"
     gene            1432..2487
                     /gene="ispG"
                     /locus_tag="ACZPD4_05750"
                     /gene_synonym="gcpE"
     CDS             1432..2487
                     /gene="ispG"
                     /locus_tag="ACZPD4_05750"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1378605.1"
                     /translation="MYRENTKEIAIGNVKIGGTHPVTIQSMTNTKTENVKETVDQILR
                     LEAAGCEIIRCAVPTMEAAEALKEIKKQIHIPLVADIHFDYRLAIAAIENGADKIRIN
                     PGNIGSEEKVQAVVDKAKEYNVPIRVGVNSGSLEKHILEKYNGVTAEGIVESALDKVH
                     MIEKMGYDNLVVSIKSSDVLMCVKAHELIAKACPYPLHVGITESGTVYSGNIKSSIGL
                     GIILNEGIGNTIRVSLTGDPAEEIRSAKLILKTLGLRKGGIEVVSCPTCGRTKIDLIG
                     LANEVEKMVQDIPLDIKVAVMGCVVNGPGEAREADIGIAGGIGEGLLIKKGEIIKKVK
                     EEKLLETLRQELLNWKK"
     gene            2574..>4525
                     /locus_tag="ACZPD4_05755"
     CDS             2574..>4525
                     /locus_tag="ACZPD4_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR1378606.1"
                     /translation="MEQVKDDKKFFHVFPTLRADDDVRLLFSDVEVKKITTNSRRDFL
                     NIYIFSRHLIQKKQIFQMEQCIKDQLFAKTAVAVHIVEEYMLSGQYTAEALMNEYRES
                     IILELKEKSMLASNMFAQADIRYEAENVVCLELLDTIVSAGRKEEIVDLLKEVYSERF
                     HIPAEIRVDYKEPDRTGSREYDEQRIQQEINAIFERRARQRGETPQAEGEEKKDQIKR
                     TSSENAADKASVSSRDGKGTSAAISGGVKKGEFKKGEFRKKDFYRPVKIGDDPNLIYG
                     RNFEDEPISLEQVITEMGEITVHGKIISFDTREIRNEKTIIIFSVTDFTDTITVKMFA
                     KNEQLPEILGELKKGAFVKVKGVTTIDKFDGELTIASVTGIKKIGDFTVQREDLSPIK
                     RVELHCHTKMSDMDGVSEVKDIVKRAHDWGHPAIAITDHGVAQAFPDANHYIETLDKD
                     DPFKVLYGVEGYVVDDLTEIAVNAGNQTLDDTYIVFDIETTGFSSIKDAIIEVGAVKV
                     TDGKITDRFSTFVNPKRPIPFEITNLTSITDEMVMDSPTIDVVLPQFLEFAGDGVLVA
                     HNAGFDVGFIEQNCRSLGLSDEFVYLDTVALARVLLPTLSKYKLNIVAKALNISLENH
                     HRAVDDAEATAEIFVKFTEMLKKDQVG"
     assembly_gap    4526..4535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4536..>5824
                     /locus_tag="ACZPD4_05760"
     CDS             <4536..>5824
                     /locus_tag="ACZPD4_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1378607.1"
                     /translation="QVGTLKEVNRYGDRNVNAIRKMPTHHIIILAKNDIGRYNLYQLI
                     SQSHMTYYARRPRIPKSLLNEHREGLLIGSACEAGELYQAVHEKRSAQQIARLAEFYD
                     YYEIQPVGNNQFMIESERIADVNSIEDLQNINREIVELGEKFGKPVVATCDVHFLNPD
                     DEVYRRIIMAGKGFDDADRQPPLFLRTTDEMLEEFSYLGAAKAREIVIDNPVKIAGMI
                     EKISPVNPNKCPPVIENSDQELRDICYRKAHEMYGEDLPKQVSARLEKELNSIISNGY
                     AVMYIIAQKLVWKSNADGYLVGSRGSVGSSFVATMAGITEVNPLSPHYYCSKCHYSDF
                     ESDEVRSFAGGCGYDMPDKKCPVCGEQLVKAGFDIPFETFLGFKGDKEPDIDLNFSGD
                     YQAKAHKYTEVLFGEGHTFKAGTIGTLADKTAYGFVR"
BASE COUNT         1895 a          942 c         1505 g         1472 t
ORIGIN      
        1 tgttaaagac agactggagt gcgttctctg ttgtgacatt gctgatcgga gtggtgacat
       61 ttgcggcagg atggtttgcg gcatcaaaat taggcggaga atgattttat agcagatgtg
      121 cgttgaattg gcaatacgaa ggattgaaaa acaacaaatt ttctgataaa ttatatataa
      181 acatataatt aatctgtaac agttgtgctc tgtagtaaag ccgaggaggt gcatcgtatg
      241 aaattagagg ttgcaataat agaaagaaaa gagcagttgg atgatctgat tccgtttcgt
      301 gtggaaaatg cattatgggg aactgagaaa tttcctccta tgtatggata tatcggattt
      361 attcctcaag atgcattcta tttgaagctg atctgtgaag aaaagaatcc tcttcgtgtt
      421 tatacggaga tcaatgaccc gatttacaga gacagcgtga tggaagctta tcttatgttc
      481 gagccggttt cttcgagagc gaatgaagaa aattatttga attttgcagt aaatgcaaac
      541 ggagtcctgc atgcggaata tggttccaga cggatgtacc gttcttattt tacagaggcg
      601 gaacgggtgg gatttgaatg tcatgcagaa gtgcatgaaa atgactggac ggcagaattg
      661 aaagttcccg ttaaaatttt ggaaaagatt tacggaccgc tgaatttagg agaaggaagc
      721 tattttacat gcaattttta taaaatttca gagtcaaaag aagccgaaca ttatgcatct
      781 tattccccta tcttatcgag tgtgccgagt ttccatgtac cggaattttt tgccgacgct
      841 aagattgtca gacagaaatg aaaaaagaaa ataaattatg cagaacagag tgctttggtg
      901 caaaacccga agcgagacag aaaaagaagg gaaacgaaat aatctatgat tttagaaaat
      961 aatgaagaga aaagaagtac gctttggaga acgattgttg caatatcatc aatacttata
     1021 gttatcgtag cggtcttttt tgtgacgaga ctctttgtcg gaaatccact tgtgggaact
     1081 tgggtcagtg aagacagcgg agaagtgatc aagatagaaa gtaacggaaa agttacgctg
     1141 acagacgagg ataaaaagtc ttttgagacg gtgcaatcag aagtggatac aaaaacgaag
     1201 gtttttacga ttcacactga ggatacacag gcggaaggac ttctctcagg aacatatgat
     1261 tacagtatag agaaagatac gcttacatta acagagcgtg aatatggcga tcagcttgta
     1321 tttgtcaaaa gagaaaagaa gtaacagcaa atcagatgaa gaagagttcc gtttacggaa
     1381 ctcttctgtt ataataaaac gataagacag ttcggaaaaa ggagaaacag aatgtacagg
     1441 gaaaacacaa aagaaattgc gatcggcaat gtgaagatcg gaggaactca tccggtaacg
     1501 atccagtcga tgacaaatac aaagacagaa aatgtgaaag aaacagtaga tcagatactg
     1561 cgtttggagg cggcaggatg tgaaatcatt cgttgtgcgg ttccgacaat ggaagcggca
     1621 gaggcgttga aagaaataaa aaaacaaatt catattcctc ttgtggcaga tattcatttc
     1681 gattacaggc tggcgatcgc cgcgatcgaa aacggagcgg ataagattag gatcaatccg
     1741 ggaaatatcg gctcggaaga aaaggttcag gctgttgtag ataaggcaaa agagtacaat
     1801 gtaccaatcc gtgtcggtgt gaacagcggt tctttggaaa agcatatttt ggaaaaatac
     1861 aacggtgtaa cggcagaagg gatcgtggag agtgctcttg acaaagtgca tatgatcgaa
     1921 aaaatgggat atgacaatct tgttgtgagt attaaatctt ccgatgtgct gatgtgcgtg
     1981 aaagctcacg aattgatcgc aaaggcatgt ccttatccgc ttcatgtcgg tattacagaa
     2041 tcgggaacgg tttattcggg aaatataaaa tcttcgatag gtcttgggat catcctcaac
     2101 gaggggatag gaaatacgat ccgggtttct ttgaccggag atccggcaga agagattcga
     2161 agcgcaaagc ttatattaaa aacacttggt ttgcgaaaag gcgggatcga agttgtatcc
     2221 tgtcctactt gcggcaggac gaagatcgat ctgatcggtc tggcaaacga agtggagaag
     2281 atggttcagg atattcctct tgatatcaaa gttgccgtta tgggatgtgt agtgaacggt
     2341 ccgggagagg caagagaagc tgatatcgga atcgccggag ggatcggaga aggtcttctg
     2401 atcaaaaaag gcgagatcat aaaaaaagta aaagaagaaa aacttttgga aacattgcgg
     2461 caggaacttt taaactggaa aaagtgacag attcggatca tgccggatga agagagctaa
     2521 aaagaatacg tattactgtt atcagtgtaa aagaatatat aggagctgtt gatttggaac
     2581 aggtaaaaga cgataaaaaa ttttttcatg tatttccgac gttgagagca gacgatgatg
     2641 tccgtttgct gttttcagat gtggaagtta agaagatcac gacgaactcg cgacgtgatt
     2701 ttcttaacat atatattttc agcagacatc tcattcagaa gaagcagatt tttcagatgg
     2761 aacaatgtat caaagatcag ttatttgcca aaacggctgt tgcggtgcat attgtggagg
     2821 aatatatgct ctcgggacag tatactgcag aagcgcttat gaatgaatac agggagagta
     2881 tcatcctcga attgaaagaa aaaagtatgc ttgcatctaa tatgtttgca caggcagata
     2941 tccgctatga agcggaaaat gtagtctgcc tggaactgct cgatacgatt gtttcggcag
     3001 ggcgaaaaga agagatagtg gatctgttaa aggaagtgta ttcagaaaga tttcatattc
     3061 cggcagagat ccgtgtagat tacaaagagc ctgacagaac aggaagcagg gaatatgatg
     3121 aacagagaat ccaacaggag atcaatgcga tctttgagag acgggcaaga cagcgcgggg
     3181 aaacgcctca ggcagaagga gaagagaaga aagatcagat aaaaagaact tcatctgaaa
     3241 atgcggcgga taaagcatcg gtatcatccc gagacgggaa aggaacatcg gcggcaatat
     3301 caggaggagt gaaaaaggga gaatttaaaa aaggtgaatt ccgaaagaaa gatttttacc
     3361 gtcctgttaa gatcggtgac gatccgaatc tgatttacgg gcgtaatttt gaagatgagc
     3421 ctatttcttt agagcaggtg atcacagaaa tgggagagat cacagtacac ggtaagatta
     3481 tcagttttga tacaagagaa atccggaatg aaaagacgat cattatattt tctgtgacag
     3541 actttacaga cacgatcaca gtgaaaatgt ttgcgaaaaa cgaacaactt cctgaaatct
     3601 taggggaatt aaaaaaagga gcgttcgtaa aagtcaaagg agttacgacg attgataaat
     3661 ttgacggaga acttacgata gcttctgtaa caggaattaa aaagatcggt gattttacgg
     3721 ttcaaaggga agatttaagt ccaatcaagc gggtagaact tcattgtcat acgaaaatga
     3781 gcgatatgga cggtgtgtcg gaagtaaaag atatcgtaaa acgtgcgcat gactggggac
     3841 atcctgcaat cgcgatcaca gatcacggtg tggctcaggc gtttccggac gccaatcatt
     3901 atattgaaac acttgataaa gacgacccgt ttaaagttct ttacggtgtg gaaggatatg
     3961 ttgtggacga tctgacggag attgcagtaa acgcaggcaa tcagacgctt gacgatactt
     4021 atattgtctt tgacatcgag actacagggt ttagttcaat caaagatgcg atcatagagg
     4081 tcggagccgt aaaagtgacg gacggaaaga tcacggatcg ttttagtacg tttgtgaatc
     4141 cgaaacggcc gattccgttt gagatcacaa acctgacaag catcacagac gaaatggtga
     4201 tggattcacc gacaatagac gttgttttac ctcagttcct tgagtttgca ggagacggtg
     4261 tgcttgtcgc gcacaatgcc gggtttgatg ttggatttat cgaacagaat tgccggtcgc
     4321 ttggactttc ggatgagttt gtatatctgg atacagtagc gctggcacgt gtacttttgc
     4381 cgacgttgtc aaaatataaa ttaaatatcg tggcgaaagc gttgaatatt tcccttgaga
     4441 atcatcaccg tgcagtggat gatgcagagg caactgcgga aatatttgtt aaatttacag
     4501 aaatgctgaa aaaggatcag gtcggnnnnn nnnnncaggt cggaacttta aaagaagtga
     4561 atcgttacgg ggaccggaat gtcaatgcga ttcggaaaat gccgacacat catattatta
     4621 ttttggcgaa aaatgatatc ggccgatata atctgtacca gttgatctct caatctcata
     4681 tgacttacta cgcaagaaga ccgagaatac cgaaaagtct tttgaacgag cacagggaag
     4741 ggctgctgat cggaagcgcc tgcgaggcgg gagaattata tcaggcagtg catgaaaagc
     4801 gctctgccca acagattgcg aggctggcgg aattctatga ttattatgag atacagcctg
     4861 tcggaaacaa tcagtttatg atcgagagtg aacgtatagc ggatgtaaat tcaatagaag
     4921 atctgcaaaa tatcaacagg gagatcgtgg agctcggaga aaaattcgga aagccggtag
     4981 tggcaacatg cgacgtacat tttttaaatc ctgacgatga agtgtaccga aggattatta
     5041 tggcgggaaa aggatttgac gacgcggatc gtcagcctcc gttgtttctt cgcacgacag
     5101 atgagatgct ggaagagttt tcctacctcg gcgcggcgaa agcgagagag attgttatag
     5161 ataatccggt gaagatcgcg ggtatgattg aaaagatctc tccggtcaat ccgaacaaat
     5221 gtcctccggt tatcgaaaat tcagatcagg aactaaggga tatctgttac aggaaagctc
     5281 atgagatgta tggagaagat cttccgaagc aagtttccgc acgtctggaa aaagaactga
     5341 attccattat ttcaaatggg tatgcggtta tgtatatcat tgcgcaaaag cttgtttgga
     5401 agtcaaatgc ggacggatat cttgtaggtt cgcgaggatc ggtcggatct tcctttgttg
     5461 cgacaatggc gggaattaca gaggtaaatc cgttaagtcc gcattattat tgttcaaaat
     5521 gccattatag tgattttgag tcggatgaag tgcgttcatt tgccggcgga tgcgggtatg
     5581 atatgccgga taagaagtgt ccggtatgcg gagaacagct tgtgaaagcg ggatttgata
     5641 ttccgtttga gacattccta ggattcaaag gagacaaaga accggatatc gacttgaact
     5701 tttccggaga ttatcaggca aaggctcata aatatacaga agttcttttc ggtgaaggac
     5761 atacatttaa ggctggaacg atcggtactc tggcagataa gacagcgtat ggatttgtcc
     5821 ggaa
//
LOCUS       DBMDXF010000118         5792 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3476_length_5792_cov_1.989281, whole genome shotgun sequence.
ACCESSION   DBMDXF010000118 DBMDXF010000000
VERSION     DBMDXF010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3476_length_5792_cov_1.989281"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..457)
                     /locus_tag="ACZPD4_05765"
                     /pseudo
     CDS             complement(1..457)
                     /locus_tag="ACZPD4_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847111.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(460..>661)
                     /locus_tag="ACZPD4_05770"
     CDS             complement(460..>661)
                     /locus_tag="ACZPD4_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378608.1"
                     /translation="PLIFLILMFSLESKIVFLILWILSIILIATWLITVEYIHNELAA
                     QQELSGMSFEEMLKLFRKMEEN"
     assembly_gap    662..671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<672..3007)
                     /locus_tag="ACZPD4_05775"
     CDS             complement(<672..3007)
                     /locus_tag="ACZPD4_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HIR1378609.1"
                     /translation="MKKSFHIFQRDLSRLLHNKAAILVLIGVCLLPSLYAWFNIAANL
                     DPYGNTSKIKVAIACLDKEASSENLSINAGKEIVKNLKENKQLGWTFTSEKEALNGVK
                     SGKYYASIVIPEDFSRSLISILSGDLQTPKLDYYINEKANAIAPKITGTGAAAIQEQI
                     NSTFSSVASESVSGILKQSASDLSSTLGSVNSDITKTLEKADANIAAYKQLLENFNAA
                     AEKSAPLITRAKSTASSLKSAASSGSSALSESSRILSETRTAAGNFSSSLSSSLTQGE
                     ILLGQAHNIASAGLTDLETAARSVNGSIGNALDFANSTAVLNGNILKELGELASLIPG
                     SDLDSAIAGLQAQNTNNQELILSLSAGNAGIQNAINTTASTREQLYTITSANIGSLHD
                     LRAVLDKNVLPQLNKTLDAFSSLTGEMNGLLTTVPSSADQIDNLLDQMSAGLSNTVTA
                     LEGTKTALSNVQEHLGTIQTDLNALSGSDIYETLLSLEGIDKQSVSAFMSSPVKIETK
                     SFYKIANYGSAMTPFYTNLAIWVGGIVLIAIFKLEVDKDSSMLRYSSASLYFGRWLLY
                     ITVGMVQGFIVCIGDVFLKGIECAHPAALIFTGVICSFVYVNIIYALSISFKHIGKAL
                     CVLLVILQIPGSSGTYPVEMTPAFFQNVHPFLPFTYGISAMRECIAGMYGSTYIHNLL
                     ILLLFVPVSLLIGLGIRPLLSGLNRLFDIKLAETDLMLGETPDRNIRRSAQLSLLLKA
                     SLSQEELQIQTAEKAQRFEKNYHKMIRFGFFAILTIPL"
     gene            3186..3776
                     /locus_tag="ACZPD4_05780"
     CDS             3186..3776
                     /locus_tag="ACZPD4_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1378610.1"
                     /translation="MEKKTDRRVRKTKNQLRTGLARLMKKKNIGQITVKELVDDVDIN
                     RSTFYLHYSDIPSLLKEIEDDMIEEIERAILEHPIKIEKDTTYLFIEDLFRVLSDNRE
                     IGCALLGPYGDITFIRRIEKFLEENSRDLLAERFPEKFAEMKYFYSFCFNGCLGFIRT
                     WLENGENMPPEYAARLTFQMVVSSLDAFYEDAKENK"
     gene            3889..5259
                     /locus_tag="ACZPD4_05785"
     CDS             3889..5259
                     /locus_tag="ACZPD4_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1378611.1"
                     /translation="MKREKFGSRLGFILISAGCAIGLGNVWRFPYITGQYGGAAFVLI
                     YLLFLLILGLPIMVMEFSVGRASRSSVALSFDRLEPLGTKWHWYKWFGMAGNYLLMMF
                     YTTIGGWLLLYFVKMAAGNFKGLDASGIKNEFGELMQQPGIMIVCMIIVVAACFAICS
                     IGLQKGVEKITKVMMLMLLALMIILAVRSVTLSGAGEGLAFYLVPDFAKVKEAGLMEV
                     VFAAMGQSFFTLSLGIGAIAIFGSYIDRSRSLTGEAVCVTVLDTCVALLSGFIIFPAC
                     FAFGVNPDSGPNLIFITLPNIFNSMAGGRIWGSIFFLCMLFAAASTIIAVFENIISFA
                     MDLTGCSRGKAVAVNLVVIILLSLPCVFGFNLLSGFQPLGAGSTILDLEDFIVSNNLL
                     PLGSLVYLLFCTSRYGWGWKNFRKEANAGTGIKFPKWSRIYVSYILPLIVLFIFVQGY
                     WSKFFG"
     gene            5376..>5792
                     /locus_tag="ACZPD4_05790"
     CDS             5376..>5792
                     /locus_tag="ACZPD4_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR1378612.1"
                     /translation="MKSNTQNAKIEAITEKTLVLGIDVGSETHYARAFDYRGVEYSKK
                     PFKFSNTEAGFVTFKAWVLDIKEKHGKDKVIPGMEPTGHYWFNLGKFLQDNEMKPVLV
                     NPHHVKKSKELDDNHPTKNDRKDPPLSGDRIGHDARI"
BASE COUNT         1671 a         1003 c         1418 g         1690 t
ORIGIN      
        1 tttttctgtt acttttgggg cgatggcgtt ctccttttcg tttgcagtaa gtaattgtcg
       61 gatgtgtcgt ttcagaagaa aacagactca tcatatcttt gctgaatgtt tcagaaatga
      121 caacagaagc ataatatttt ccggaacgta caccgttaga agcgtccttg gcatctgtaa
      181 atatccagtg aagctgtgtg ttttcacgca gtgaagaaat aatgtcattt ccgagattga
      241 gccggaccgg aagcagttcg ccgtcatatc ctttatcaag acttgcgacg gctactttta
      301 aatttccggt atttttgtac ggatcccaac ttgcggcaat attaaaccat gcatagagag
      361 agggaacgat agtaatgccg aaaataacga tcagcgcgat tacgtttttt cggatattgt
      421 ggatgtcgtt tcgcgcaatg cgtatgatat ttttcatagt cagttttcct ccatttttct
      481 gaataatttc aacatttctt caaatgacat accggacagt tcctgttgag ctgcaagttc
      541 gttatgtatg tattccactg tgatcagcca tgtggcgatt aaaataatag ataagatcca
      601 aagaatgagg aatacaattt tagattcaag gctgaacata aggatcagga aaatgagcgg
      661 annnnnnnnn natgagcgga atagttaaaa tcgcaaaaaa tccaaaacgg atcattttgt
      721 gataattttt ttcaaaacgc tgtgctttct cggcagtttg gatctgaagt tcttcttgtg
      781 ataaagaagc ttttagtaaa agagaaagct gtgcgctgcg tctgatattt ctgtcgggtg
      841 tttcaccaag cattaaatcg gtttctgcaa gtttaatgtc aaaaagccgg ttcagacctg
      901 aaagcagagg tctgatccca agtccgatca gtaaggaaac gggaacgaaa aggagcagga
      961 tgagcagatt gtgtatataa gtgcttccgt acattccggc aatacattct ctcattgcgg
     1021 aaataccgta agtaaagggt aggaacggat ggacgttttg gaaaaacgcg ggtgtcattt
     1081 ctacaggata tgtgccggat gatccgggaa tctgaaggat gacgaggaga acacataatg
     1141 ctttcccgat gtgtttaaat gagattgaca gtgcataaat aatattgaca tacacaaacg
     1201 aacagatcac tccggtaaag ataagagcgg ctggatgcgc gcattcaatc ccttttagaa
     1261 aaacatctcc gatacagaca atgaatcctt gcaccattcc gaccgtgata tacaaaagcc
     1321 agcgtccgaa atagagagag gcagaagaat atcgaagcat agagctgtct ttatctactt
     1381 ctaatttaaa aatcgcgatg aggacgatgc cgccgaccca aattgcaagg tttgtgtaaa
     1441 aaggcgtcat tgccgagccg taattagcta ttttataaaa actctttgtt tctattttca
     1501 caggtgagga cataaatgcc gataccgatt gcttgtcgat tccttctaat gagagaagag
     1561 tttcgtagat gtcagaaccg gaaagggcat ttaagtctgt ttgaatcgta ccgagatgtt
     1621 cctgaacatt tgacagggcg gtttttgtgc cttcaagagc cgttacagta tttgaaagtc
     1681 cggcgctcat ttggtctaac aggttgtcga tctgatcggc ggaggaagga actgttgtta
     1741 gaagaccgtt catttctcct gtcaaagaag agaaagcatc taatgtcttg tttagctgcg
     1801 gcagaacgtt tttgtccaaa acagcgcgga ggtcgtgcag actgccgatg tttgcggatg
     1861 taattgtgta aagctgttcc cgggtagaag cggttgtgtt gatggcattt tgtattcctg
     1921 catttccggc gcttaaagag aggatcagtt cctgattatt tgtgttttga gcctgcagtc
     1981 cggcgatggc agaatccaaa tcagatccgg gaatgagtga ggcgagctct cctaactctt
     2041 ttaaaatatt tccgtttaag acggcagtgg aattggcaaa atccagtgcg tttccgatcg
     2101 atccgtttac cgagcgggcg gcagtttcaa gatcggtaag tcctgctgat gcgatattgt
     2161 gcgcctgacc gagcaaaatt tctccttgtg tcaaagatga agatagggat gaagaaaagt
     2221 ttcctgcggc agtacgggtt tcagagagga tgcgggaaga ttcgcttaga gccgaagaac
     2281 ccgacgaggc ggcggatttt aaagatgaag cagttgattt tgcgcgtgtg atcagggggg
     2341 cagatttttc tgcggctgca ttgaagtttt cgagaagttg tttgtaggcg gcaatgtttg
     2401 catcagcttt ttcaagtgtt ttagtaatat cggaattgac agatccaagt gttgacgaca
     2461 agtctgacgc agactgcttt aatatgccgg aaacggattc agaagcaacc gaagagaagg
     2521 tgctgttgat ctgttcctga attgcggctg cgcctgtgcc tgtgattttg ggagcaatgg
     2581 cattggcttt ttcatttatg taatagtcaa gtttcggcgt ctgtaagtcg ccggataaaa
     2641 tactgatcaa agagcggctg aaatcttcgg gaataacaat agaagcataa tattttcctg
     2701 attttactcc gttgagagcc tctttttccg aagtgaacgt ccatccgagc tgtttgtttt
     2761 cttttagatt tttgacaatt tcttttccag cattgataga aagattttcg gaagatgctt
     2821 ctttatctaa acaggcaata gcgactttaa tcttagacgt atttccgtat gggtctaaat
     2881 tggctgcgat attaaaccat gcgtatagag aaggcagcag gcatacgccg atcagaacaa
     2941 gaatcgccgc tttattgtgt aaaaggcggg agaggtcacg ctgaaaaatg tgaaatgatt
     3001 ttttcataat atcagaactc cttatatatt aatagataca atgttgattt tcttgataaa
     3061 gagtattata ttaaggtaga aatagattta caaccattaa tagaagtgtg aaatgtcgga
     3121 taaacgacaa tataacgaaa tgtgttgaca catgtgaaga gatattatga cgaggagaaa
     3181 gcgatatgga aaaaaagaca gaccgcaggg tgagaaaaac taaaaaccag cttcgtacgg
     3241 gactagcccg tcttatgaag aaaaaaaata tcggacagat taccgtaaaa gaacttgtag
     3301 atgatgtgga tattaaccgt tcgacatttt atctgcatta ttcggatatt ccgtctcttc
     3361 taaaagagat agaagatgat atgattgagg aaatagaaag agcgattctg gagcatccga
     3421 ttaaaatcga gaaagacact acatacttgt ttatagaaga tttgtttcgg gtgttaagcg
     3481 ataacagaga gatcggatgc gcgcttcttg gtccatacgg tgatattacg tttatcagga
     3541 gaattgaaaa atttttggaa gaaaacagtc gtgatctttt ggcggaacgc tttcccgaaa
     3601 agtttgcgga aatgaaatat ttctattcgt tttgttttaa cggatgtctt ggatttatta
     3661 gaacatggct tgaaaacggg gaaaatatgc cgcctgaata tgcggctcgt cttacatttc
     3721 aaatggttgt aagttcgctt gatgcttttt atgaagatgc gaaagaaaat aaataaatat
     3781 tttgtaaata atggtggaaa aaaagtgtgt atatatggta cattaatatc tgtacagttg
     3841 taacactgta cagtattaaa atggtatata aggaagagag gaaattttat gaagagagaa
     3901 aaattcggct cccgccttgg attcattctg atttcggcag ggtgtgcgat cggtcttgga
     3961 aatgtatgga ggtttcctta cattaccggg cagtacggag gagcggcatt cgtattgatc
     4021 tatttgctgt ttcttctgat acttggattg ccgatcatgg taatggagtt ttcggttgga
     4081 agggcatcaa gaagcagcgt tgcgttatca tttgacagat tagagccgct tggtacaaaa
     4141 tggcactggt ataaatggtt cggcatggca ggaaattatc tgcttatgat gttttacaca
     4201 acgatcggcg gatggctgct tttgtatttt gtaaaaatgg cagcgggaaa ttttaaaggc
     4261 ttggatgcgt cgggaatcaa aaacgagttt ggcgagctga tgcagcagcc gggaattatg
     4321 atcgtatgta tgatcattgt tgtggcagcc tgctttgcga tttgcagcat cgggctgcag
     4381 aaaggcgtag aaaaaatcac aaaagtgatg atgcttatgc ttcttgcgct tatgatcatt
     4441 ctggcggtac gttccgttac tctttcaggg gcaggcgaag gactggcgtt ttatcttgtt
     4501 cctgactttg ccaaagtaaa agaagcggga ctgatggaag ttgtatttgc ggcaatgggg
     4561 caatcgttct ttacattgag ccttggaatc ggcgcgatcg cgattttcgg aagctacata
     4621 gaccgctcca gaagtctgac aggagaagca gtatgcgtaa ccgttcttga tacatgtgta
     4681 gcgctgctct cgggatttat tattttcccg gcatgttttg ctttcggagt gaacccggac
     4741 agcggtccga atcttatttt tattacgttg ccgaatatat ttaattctat ggcaggaggc
     4801 agaatttggg gaagtatctt tttcctctgt atgctttttg cggcggcgtc aacgattatt
     4861 gcggtatttg aaaatatcat ttcatttgcg atggatctga ccggatgctc gagaggcaag
     4921 gcagtggcag tgaatctggt tgtgattatc ctgctttcac ttccatgtgt gtttggattt
     4981 aacctgctga gcggattcca gccgctcgga gcgggaagca cgatccttga tctggaagat
     5041 tttatcgtca gcaataacct tcttccgctt ggaagtcttg tatatcttct gttttgtaca
     5101 agccgttatg gctggggatg gaagaacttc cgcaaggaag ctaatgcggg aacgggaatt
     5161 aaattcccaa aatggtcacg gatctatgtg tcatatatcc ttccgctgat cgtgttattt
     5221 atcttcgtac agggatattg gtctaagttc ttcggatagg gagcttcctg tataataaaa
     5281 aatgtggatg ttgaaaattg aaaatacctc agagtacaat aagactactg actggcaggt
     5341 tagtaaaaat cttattgaac agagaggtat ctacaatgaa gtctaacaca cagaacgcaa
     5401 aaattgaagc tattacagaa aaaactttgg tactcggaat cgatgtcgga agtgaaacgc
     5461 attatgccag agcttttgat tacagaggag tcgagtattc gaagaaacca ttcaaattta
     5521 gtaacacgga agcaggattc gtgacattta aggcatgggt tctggatatc aaagaaaagc
     5581 atgggaagga taaggtgatt ccagggatgg aaccaactgg tcattactgg tttaatcttg
     5641 gaaagtttct tcaagataat gaaatgaaac cggttcttgt aaatcctcac catgtgaaga
     5701 aatcaaaaga actcgacgac aatcatccga caaagaatga tcgtaaggat ccaccacttt
     5761 ccggtgacag gatcggccat gacgctcgga tc
//
LOCUS       DBMDXF010000119         5655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3566_length_5655_cov_1.951566, whole genome shotgun sequence.
ACCESSION   DBMDXF010000119 DBMDXF010000000
VERSION     DBMDXF010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_5655_cov_1.951566"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..588
                     /gene="fic"
                     /locus_tag="ACZPD4_05795"
                     /pseudo
     CDS             1..588
                     /gene="fic"
                     /locus_tag="ACZPD4_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845958.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
     gene            860..1915
                     /locus_tag="ACZPD4_05800"
     CDS             860..1915
                     /locus_tag="ACZPD4_05800"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR1378613.1"
                     /translation="MIGRVCGTGSYLPIRTVSNDELSHVMETSDEWIRERTGIRFRHV
                     ALEETTSDMAAEAAARALKNGEVNADEIELILTATSSSDVIYPSAACIVQKRIGAQNA
                     LAFDLNGACSGFVIAFNTAQAYIRAGIYKTILVVGAESMSRLVDWSDRSTSVLFGDGA
                     GALILKAEEGDHYWQTGHSDGTKDFVLTLRSKSLFEEQDDNPKDVLSKSDEGSSVEEH
                     DDVSKNAEDISRKKKSHKTGICMDGQEVFKFAVRKVPELTEELLEKSGLKIADIDFFI
                     LHQANERIVKAAAKKLKVDMSRFPINMENYANTSAASIPILLDELVQTGRLRKGQKIL
                     LAGFGAGLTWAGSILEW"
     gene            1993..2220
                     /locus_tag="ACZPD4_05805"
     CDS             1993..2220
                     /locus_tag="ACZPD4_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1378614.1"
                     /translation="MTERMKEIVAEALNVEIETLKEDTSFKEDLGADSLDLFELVMAF
                     EEEFGVEIPSEDLEKITTIKDAAAYMEAHKE"
     gene            2311..3249
                     /gene="fabK"
                     /locus_tag="ACZPD4_05810"
     CDS             2311..3249
                     /gene="fabK"
                     /locus_tag="ACZPD4_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HIR1378615.1"
                     /translation="MKTEITELLGIEYPIIQGGMAWVAEYHLAAAVSEAGGLGIIGAA
                     SAPPEWVRDQIQKAKKMTDKPFGVNIMLMSPYADEIAKVIVEEKVKVVTTGAGSPEKY
                     MQMWKEADVKVIPVVASVALAKRMERCHADAVVAEGTEAGGHIGEASTMTLVPQVVDA
                     VKIPVIAAGGIADGRGIAAAFMLGAKGVQMGTAFIVTEECQVHENYKNEIIRARDIDS
                     KVTGRSCGHPVRTLRNQMTKLYLQKEKEGASFEELEALTLGGLKKAVVEGDVKNGSLM
                     AGQSAGLVTEKMTCEMLIRKLVKGTEKQIRGSVFYE"
     gene            3242..4162
                     /gene="fabD"
                     /locus_tag="ACZPD4_05815"
     CDS             3242..4162
                     /gene="fabD"
                     /locus_tag="ACZPD4_05815"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR1378616.1"
                     /translation="MSKTAFIFPGQGAQTAGMGKDFYENSRTAKDIFDMASELLSLDM
                     RKLCFEKNDLLDKTEYTQAALVTVSMAAEHVLREWGMTADVTAGLSLGEYCAAASAGA
                     FSTEDAVLMVRKRGIFMEEAVPANKGAMAAVIGLTGARTEEVVEKIPNVWIANYNCPG
                     QLVITGEKSAVETAGSSLKEAGAKRVILLNVSGPFHSPMMETAGQQLARVLENVRLNQ
                     PVIPYVSNVTADFVGETGEIKDLLIRQVSSPVRWQQSVEKLIAAGVDTFVEIGPGKTL
                     SGFIRRIDRNVKCFNVGTWEEAEKAAEQLC"
     gene            4188..4928
                     /gene="fabG"
                     /locus_tag="ACZPD4_05820"
     CDS             4188..4928
                     /gene="fabG"
                     /locus_tag="ACZPD4_05820"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR1378617.1"
                     /translation="MLKGKIAVVTGASRGIGRAIAVKLAAEGAVVIINYNRSKDEAEK
                     VKEQIEASGGQVKLIQCNVASFESCEKMFEEVMKEFGRIDILVNNAGITKDGLLMKMS
                     EDDFDAVVDTNLKGAFHCIRFVSRAMIRQRSGRIINISSVSGVLGNAGQANYAASKAG
                     VIGLTKSAARELAGRKITVNAVAPGFIRTEMTAVLSEKVKEKAVLQIPMERFGEAEDV
                     AQAVAFLASEQAGYITGQVLCVDGGMAM"
     gene            4960..>5655
                     /locus_tag="ACZPD4_05825"
     CDS             4960..>5655
                     /locus_tag="ACZPD4_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR1378618.1"
                     /translation="MKRRVVVTGLGAVTPIGNTVDVFWDEIKKETVGIGPITQFDVSD
                     YKVKIAAQVKGFDARERLDFKSAKRMELFTQYAAAAAKEAFEDAGIDMEKEDPYRAGV
                     IVGSGVGSLKCVEDNYETILKKGPNRVHPLMVPLMISNMAAGNISIRLGLRGKCTNVA
                     TACASGSHCIGDAFRAIQYGDADIMVAGGAESAISPTGLAGFTALTALSTSEDPTCAS
                     RPFDKDRDGFVLGE"
BASE COUNT         1879 a          872 c         1545 g         1359 t
ORIGIN      
        1 ctcggcatta ccgattctgt agaatgagcc aaagctgaag aaaaaatcag caaaaaaaga
       61 gcaatagaac tttttgaaag cggctatttg aattatctta agacgggaac ttttaaatca
      121 cttgctgaaa tacacaaata tttatttggt ccgatttatg gttttgccgg acagataaga
      181 actgttaata tttcaaaagg taattttcgt tttgctccga ttatgtattt agaagcagct
      241 cttgaaaata tcgaaaaaat gcctcaatca acatatgatg aaattataga aaaatatgtg
      301 gaaatgaata ttgcacatcc ttttcgtgag ggaaacggca gaagtacaag aatttggctt
      361 gatttgattt taaaaaaaga attaggtcag gtaatcgatt ggagccgtgt agataaagag
      421 gactatcttt ctgccatgga acgcagtcct gttaaagata ttgaaatcaa acatatatta
      481 aagcaggcgc tgacgaacag aatcaatgac cgtgaggttt atatgaaagg tatcgatcac
      541 agttattatt acgaaggcta ttccacattt aagataagtg atttgtaatt aaaatatttc
      601 tatcaatggc agaagttaat ataaagaact gccgttgcaa gtcgttctct aagtaaaatt
      661 caaaggagaa tcgattgtga agagagtggc aagcgaaaga aacctatttg caaagataac
      721 ctgagcttgc tgctctaaat tattgtttac ggattgaacg gaaagattat gaaaaaaaca
      781 gttgacaaat tattttttag tgttatactt tgaaagtcaa aatgtttaag aatcaaataa
      841 aatatgacgg agaagaaaaa tgataggaag agtttgtgga acgggatcgt atttaccgat
      901 tcggacagta agcaatgatg aactatcgca tgtcatggag acgtcggatg aatggatacg
      961 agaacgaacc ggaatcagat tccggcatgt ggcattagaa gagacaacat cagacatggc
     1021 ggcggaggct gcggcccggg cgttgaaaaa cggagaagta aatgcggatg agatcgaatt
     1081 gatcttaact gcgacatctt cttcggatgt gatttatcca tcggctgcgt gtattgttca
     1141 aaagagaatc ggcgcccaga acgcgttggc atttgatctg aacggagctt gttcgggatt
     1201 tgtgattgct ttcaatacag cgcaggcata tattcgcgca ggaatctata aaacgattct
     1261 tgtagtcggc gcagaaagca tgagtcgcct tgtggattgg agtgacagga gcacatctgt
     1321 attattcggc gacggcgcag gcgcgctgat actgaaagcg gaagaaggtg atcattattg
     1381 gcagacaggt cattctgacg gcacgaaaga ttttgtgctg acattaagat caaaaagtct
     1441 gtttgaagaa caggatgata atccaaaaga tgtcttatca aaaagtgatg aaggatcgtc
     1501 agtggaagaa cacgatgacg taagtaaaaa tgcagaagat atcagccgga aaaagaaaag
     1561 tcataaaaca ggaatttgca tggatgggca ggaagtattt aaatttgcgg tcagaaaagt
     1621 accggagctg acagaagagc ttttagaaaa gtcgggactg aaaattgcag atattgattt
     1681 ttttattttg catcaggcga atgaaaggat tgtgaaagcg gcggcaaaga aattaaaagt
     1741 cgatatgagc cgttttccga taaatatgga aaattatgca aatacatctg ccgcatcgat
     1801 tccgatcctt ttggatgaac ttgttcaaac gggaagattg agaaaaggtc aaaaaatatt
     1861 gcttgccgga tttggcgcag ggctgacatg ggctggaagt attttagaat ggtaaatctc
     1921 tttgagagat caaacgaaaa caaattgtgg ataaaaaata aagtaaaaga gaaaagaaag
     1981 gaagaaatta tcatgacaga gagaatgaaa gaaatcgtag cagaggcatt gaatgtagaa
     2041 atcgaaactt taaaagagga cacatcattt aaagaagatt tgggggcaga ttcacttgat
     2101 ctttttgaat tggtgatggc gtttgaggaa gaattcggag tagagattcc ttccgaagat
     2161 ctggaaaaga tcacaacgat caaagatgcg gcggcatata tggaggctca caaagagtaa
     2221 aaaataaaaa accggatata aaaaatgaaa atagaaacaa agaggccgat aataaaaaag
     2281 aaaaaagtat caggagaaag tattgtcaaa atgaaaacag aaataacgga acttttggga
     2341 attgaatatc cgatcattca gggcgggatg gcatgggttg cagaatatca tctggcagcg
     2401 gcagtatccg aggcaggcgg tctgggaatt atcggagcgg caagcgctcc tcctgaatgg
     2461 gtgagagatc agatacaaaa ggcaaagaag atgacagata aacctttcgg agtcaacatt
     2521 atgcttatga gtccgtatgc agatgaaatt gcaaaggtaa ttgtggaaga aaaggtaaaa
     2581 gttgttacaa caggagccgg ttctccggaa aaatatatgc agatgtggaa agaagcggat
     2641 gtaaaagtga ttccggttgt tgcgtctgtt gcacttgcaa agagaatgga acgctgtcat
     2701 gccgatgcgg ttgtcgcaga aggaacggaa gcaggagggc atatcggaga ggcatcaaca
     2761 atgactcttg taccgcaagt tgtagatgcg gtaaagattc ctgtcattgc ggcaggagga
     2821 attgcagacg gaagagggat tgcggctgca tttatgctcg gagcaaaagg agttcagatg
     2881 ggaacggcat ttatcgtaac agaggaatgt caggttcatg agaattataa aaatgaaatt
     2941 ataagggcaa gagatatcga ttcaaaagtt accggaagat catgcggaca tccggtgcgg
     3001 acgcttagaa atcagatgac gaaactgtat ctgcaaaaag aaaaagaggg cgcgtctttt
     3061 gaagagctgg aagcattgac acttggcggt ttaaaaaaag cagtcgttga aggtgacgta
     3121 aaaaacggaa gcctgatggc aggacagtcg gcaggacttg tcacagagaa gatgacatgt
     3181 gaaatgctga tccgaaagtt agtaaaagga acagaaaaac aaatcagagg aagtgtattt
     3241 tatgagtaag actgcattta tttttccggg tcagggcgca cagacagccg gaatgggaaa
     3301 agatttctat gagaacagca ggacggcaaa agacatattt gatatggcgt ctgaattatt
     3361 gtcgcttgat atgcggaagt tgtgttttga gaaaaatgat cttcttgata agacagaata
     3421 tacacaggcg gctttggtaa ctgtcagtat ggcagcagag catgtattaa gagaatgggg
     3481 gatgactgcg gacgttacag cgggattgag cttaggcgaa tattgcgcgg cagcatcggc
     3541 aggagcattt tctacagaag acgcagttct gatggtgaga aaacgcggga tttttatgga
     3601 agaagccgtt cccgcaaaca aaggggcgat ggcggctgta atcggactta caggcgctcg
     3661 gacagaagaa gtagtggaaa agattccgaa tgtatggatc gcaaattata actgtcccgg
     3721 tcagttggtg atcacaggag aaaaaagcgc agtggaaaca gcgggaagta gcctgaaaga
     3781 agcaggagca aagagagtta ttttattgaa tgtgagcggt ccgtttcatt ctccgatgat
     3841 ggaaactgca ggacaacagc ttgcgcgtgt tttggagaat gtcaggctga atcaaccggt
     3901 cattccttat gtatcgaatg tgactgcgga ttttgtggga gaaacaggag agattaagga
     3961 tcttttgata cggcaggtat catctcctgt gagatggcag caaagtgtgg aaaaactgat
     4021 cgccgcaggg gtagatacat ttgtggagat cggaccggga aagacattgt ctggatttat
     4081 acggcggatt gacagaaatg taaaatgttt caatgtcggt acatgggaag aagcagaaaa
     4141 agcggcagaa cagttatgtt aaagcgacag aaagtggaag gaaaatgatg ttaaaaggga
     4201 aaattgcagt tgtaaccggg gcatcccggg ggatcggcag agcgatcgcg gtgaaacttg
     4261 cggcagaagg cgcagtcgtg atcattaatt ataacaggtc gaaagacgaa gctgaaaaag
     4321 taaaagagca gattgaagcg tcgggaggac aggtaaaact gattcagtgt aacgtagctt
     4381 cttttgaatc atgcgaaaag atgtttgaag aggttatgaa agagtttgga agaattgata
     4441 ttcttgtcaa caatgcggga attacaaaag acggtttgct tatgaagatg tcggaagatg
     4501 attttgatgc ggtcgtagat acaaacttaa agggggcgtt tcactgtata cgctttgttt
     4561 caagagcaat gatacgtcaa agaagcggaa ggatcattaa tatctcttcg gtgtccgggg
     4621 tacttggaaa tgcaggtcag gcaaattatg ccgcatctaa ggcaggggtg atcggtctga
     4681 caaaatcagc ggcgagagaa cttgcaggaa ggaaaattac agtaaatgca gtcgcgcccg
     4741 gatttatccg gacggaaatg acagcggttc tttcggaaaa agtgaaagaa aaggcagttc
     4801 tgcaaattcc gatggagcga ttcggagagg cggaagatgt ggcgcaggct gttgcgtttc
     4861 ttgcatcaga gcaggccgga tacattaccg ggcaggtgct ttgcgtagac ggaggcatgg
     4921 cgatgtaatt ataatcgctc cgcttgaaaa ggaggaagta tgaagagaag agttgttgta
     4981 accggtcttg gagctgttac tccgatcgga aatacagtgg atgttttttg ggatgagatt
     5041 aaaaaagaaa ctgtcggaat cggaccgatc actcaatttg acgtttctga ttataaagtg
     5101 aagatcgcag cgcaggtaaa aggatttgat gcaagagaac ggcttgattt taaatctgca
     5161 aaacgaatgg aattgtttac acaatacgcg gcggcagcag cgaaagaggc gtttgaagat
     5221 gccggaatcg acatggaaaa ggaagatcct tacagggcag gcgtgatcgt cggatccggt
     5281 gtgggaagct taaaatgtgt ggaagataat tatgagacga tcctaaagaa agggccgaac
     5341 agagttcatc cgctgatggt tccgctgatg atttcaaata tggcggcggg aaatatttcg
     5401 atccggcttg gacttcgggg aaaatgcaca aatgtcgcga cggcatgcgc ctccggttca
     5461 cattgtatcg gagatgcttt tcgggcaatc cagtatggtg atgcagatat catggttgca
     5521 ggaggagcgg agagcgcgat ctctccgaca ggtctggcag gattcacggc gcttacggcg
     5581 ctttctacat cagaagatcc gacttgtgca tcaagaccgt ttgacaaaga cagagatgga
     5641 tttgtcttgg gagaa
//
LOCUS       DBMDXF010000120         5626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3590_length_5626_cov_1.707330, whole genome shotgun sequence.
ACCESSION   DBMDXF010000120 DBMDXF010000000
VERSION     DBMDXF010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_5626_cov_1.707330"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="ACZPD4_05830"
     CDS             complement(<1..539)
                     /locus_tag="ACZPD4_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR1378619.1"
                     /translation="MNSIIETKDLKLWYGDHQVLKGITMDIPEHQITALIGPSGCGKS
                     TFLKTLNRMNDLVENVRITGEVKYDGKDIFSSNANVNELRKEIGMVFQKPNPFPMSIY
                     DNVAFGPRTHGIRSKVKLDEIVERSLRDAALWDEVKDRLKTSALGMSGGQQQRLCIAR
                     ALAVQPKVILCDESTASLDP"
     gene            complement(660..1187)
                     /locus_tag="ACZPD4_05835"
                     /pseudo
     CDS             complement(660..1187)
                     /locus_tag="ACZPD4_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
     gene            complement(1212..2072)
                     /gene="pstA"
                     /locus_tag="ACZPD4_05840"
     CDS             complement(1212..2072)
                     /gene="pstA"
                     /locus_tag="ACZPD4_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1378620.1"
                     /translation="MSNKMTVKDRLKTYRKHPGSFLVMLLVMLGALLTFSVLLFLIAY
                     ILINGVPHIKLSLFEWNYTSENASVVPALINTIVMTILSLLIAVPFGIFSAIFLVEYS
                     GKGNKFVEVIRLTTETLSGIPSIVYGLFGMLFFVNTLDWGFSILAGAFTLSIMILPLI
                     MRTTEEALKSVPDSYREGSFGLGAGKLRTVFRIVLPSAVPGILAGVILAIGRIVGETA
                     ALIYTAGSVAQIPKSVMGSGRTLAVHMYHLSSEGLYMDQAYATAVILLVLVVGINTLS
                     GMIARKLTKA"
     gene            complement(2065..2958)
                     /gene="pstC"
                     /locus_tag="ACZPD4_05845"
     CDS             complement(2065..2958)
                     /gene="pstC"
                     /locus_tag="ACZPD4_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1378621.1"
                     /translation="MKSKAWTEKFMQGVFFVAACASVLAVALICIFLFANGIPAIKEI
                     GFVKFITGEMWRPSNELFGIFPMIIGSLYVTAGAVIIGVPIGILTSVFMAMYCPKKIY
                     RPLKAATELLAGIPSVVYGFFGLVVVVPIIRDFGRALKDAGLIKNAGDGNSILTTSLI
                     LAMMILPTIIGTTESAMRAVPAHYYEGALALGATKERSIFRVIIPAAKSGVIAGIVLG
                     IGRAVGETMAVIMVAGNQPRLVGNLFKGIRTLTGNIVMEMGYATGLHREALIATGVVL
                     FVFILIINFSVALLKRRTDHE"
     gene            complement(3017..3940)
                     /locus_tag="ACZPD4_05850"
     CDS             complement(3017..3940)
                     /locus_tag="ACZPD4_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1378622.1"
                     /translation="MKMKKFIAVVSMVGMLAAAMTGCGSGSDDAKDADKGGDAKYAGD
                     WDSANDITVVSREDGSGTRGAFIELFGIQEEVDGEKVDMTTTEAQITNNTSVMMTTVE
                     GNEYAIGYISLGSLDEDAVKALKIDGAEATAENIENGSYKVSRPFNVAVKKDLDNEAA
                     KDFMSFIMSTEGQKVVEEAGYIPVSDVQAYAGTKPEGSVVVGGSSSVAPVMEKLVEAY
                     KKVNPNANVELQSSDSTTGMTSAIEGSYDIGMASRELKDEEKEKLEGTVIATDGIAVI
                     VNKANPTEEMTSDQVKSIYLGDVLTWDEIAE"
     gene            complement(4269..4341)
                     /locus_tag="ACZPD4_05855"
     tRNA            complement(4269..4341)
                     /locus_tag="ACZPD4_05855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4306..4308),aa:Ala,seq:cgc)
     gene            complement(4342..4686)
                     /gene="ssrA"
                     /locus_tag="ACZPD4_05860"
     tmRNA           complement(4342..4686)
                     /gene="ssrA"
                     /locus_tag="ACZPD4_05860"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4822..5626)
                     /locus_tag="ACZPD4_05865"
                     /pseudo
     CDS             complement(4822..5626)
                     /locus_tag="ACZPD4_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843505.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
BASE COUNT         1481 a         1447 c          947 g         1751 t
ORIGIN      
        1 ggatcaagcg atgctgtact ctcatcacaa agaattactt ttggctgtac tgccaacgct
       61 cttgcaatac aaagtctctg ctgctgtcct cccgacatcc ccaatgcaga tgtttttaac
      121 cgatctttca cttcatccca gagagccgca tctcttaaag agcgttccac aatttcatcc
      181 agtttcactt tagaacgaat accatgcgtt cttggtccaa aagccacatt gtcatagatg
      241 ctcatcggaa atggattcgg tttctgaaat accattccga tctctttgcg cagttcattt
      301 acattcgcat tagaagaaaa tatatccttt ccatcgtatt ttacttctcc tgtaatgcgc
      361 acattctcta caaggtcatt cattcggttt agtgttttca aaaacgtgga tttaccgcag
      421 ccactcggtc caatcaatgc agtaatctga tgttccggga tatccattgt aattcccttt
      481 aacacctgat gatctccata ccataatttc agatctttag tttctataat tgagttcatt
      541 tctgtctcct ttttgtcaat tctatattac aacgtgatac ccaaactctt ccatattccg
      601 tgtatcatgt acttatattc atagcaaaat ctacgctata cacgctatgc acatgcccta
      661 agtgtactat aattttcgtc cattagaaga acttccggtt caaccgcaag cgctcgcgct
      721 atgcaaagtc tttgctgctg tcctccggac attccaagag cgctcttttt cagcctgtct
      781 ttacattcat cccaaatcgc cgcatttcta agagaacgct ccacaatatc atccaactta
      841 gacttggaac ggattccatg cgttctcggt ccaaacgcaa tattatcgta aatgctcatc
      901 ggaaacggat tcggcttctg aaataccatt ccgacacgct ttctcagaag attaacgtcc
      961 atccctttaa aaatattttg tccgtccaac gcaatttcgc cttctatacg gcagccctct
     1021 acaagatcat tcattcggtt gatcgacttt agcagcgttg attttccgca gccggacggt
     1081 ccgataaacg cagtgatctt attttcttcg atttccagat ttatattttt aagggcttta
     1141 aaatccccgt aatacagatc aagattttta atacttatct ttgacatacc tgtttcctct
     1201 cttaattcac cttatgcttt tgtcagtttc cgcgcgatca tacctgacaa tgtgttaata
     1261 cctactacta aaaccaacag aataaccgct gttgcatatg cctgatccat ataaagacct
     1321 tcacttgaca aatgatacat atgaaccgcc aatgtacgcc ccgatcccat cacacttttc
     1381 ggaatctgag ccacgcttcc cgctgtatag atcagcgccg ccgtctcacc gacgattctt
     1441 ccgattgcaa ggatcactcc ggcaagaatt cccggaacag ccgacgggag cacaatgcga
     1501 aatactgtac gaagctttcc cgctccaagt ccgaagctgc cttctctgta agaatccgga
     1561 acagacttaa gcgcttcttc cgtcgttctc ataataagcg gaaggatcat aatagacaat
     1621 gtaaacgctc ctgccaaaat cgaaaatccc cagtccagcg tatttacgaa aaacaacatt
     1681 ccgaacaatc cgtatacaat agacggaatt cccgaaagcg tctctgttgt cagacggatc
     1741 acttctacga atttatttcc ttttcccgag tattccacaa gaaagattgc tgaaaaaata
     1801 ccgaacggaa ctgcgatcaa aagagaaagt atcgtcatta cgatcgtatt gataagcgcc
     1861 ggaacaacgg aagcattttc cgaagtataa ttccattcaa aaagcgaaag cttaatatga
     1921 ggtactccgt tgatcaaaat atatgcgatc aaaaacaaca gtaccgaaaa cgtaagcagc
     1981 gctcctaaca tcacgagcag cattacgaga aatgatcccg gatgttttct gtatgttttc
     2041 agcctgtctt tgactgtcat tttattactc atgatctgtc ctcctcttaa gcagtgcaac
     2101 gctgaaatta ataatcagga taaatacaaa aagaacaacg cctgttgcga tcagcgcttc
     2161 tctgtgaagt cctgtcgcgt atcccatctc cattacgata ttacctgtca gcgttctgat
     2221 tcctttaaac agatttccga caagacgggg ctgatttccc gcaaccatga tcactgccat
     2281 cgtctcgccg accgcacgcc cgatcccgag aacaattcct gcgatcacac ctgattttgc
     2341 cgcagggatg atcactctga aaatacttct ttcttttgtt gctcccaacg caagcgctcc
     2401 ttcatagtaa tgagccggga cagcccgcat cgcgctttct gttgttccga taatcgtcgg
     2461 aaggatcatc atggcaagga tcagagaagt cgtaagaata ctgtttccgt cacctgcgtt
     2521 ttttataagt cctgcatctt ttaatgctct tccgaaatcg cggatgatcg gaacaacgac
     2581 aacaagtccg aaaaatccat atacgacaga aggaattccc gccagaagtt ctgttgccgc
     2641 tttcagaggt ctgtaaattt ttttcggaca gtacattgcc ataaatacag aagtaagaat
     2701 gccgatcggc acaccgatga taactgcgcc cgccgtaaca tacaagcttc cgatgatcat
     2761 aggaaagata ccgaacagtt cattactcgg acgccacatt tctccggtaa tgaatttcac
     2821 aaatccgatt tctttaattg cagggatgcc gttagcgaat aaaaagatac atatgagagc
     2881 taccgccagt accgaggcac aggcggcaac aaagaacact ccctgcatga atttttcagt
     2941 ccatgctttt gatttcatac taatttcctc ttcattttat ctgatcttgc cgaacagttc
     3001 tgtcacacgc cgagccttac tcagcgatct catcccatgt aagtacgtct ccgaggtaga
     3061 tggattttac ctgatcagat gtcatctctt ctgtcgggtt cgctttgttt acgatcactg
     3121 cgataccgtc tgtcgcaatg actgttcctt ccagtttctc tttttcttcg tctttcagct
     3181 ctcttgatgc cataccgata tcatagcttc cttcgatggc agatgtcata cctgtcgttg
     3241 aatcggatga ctgaagttca acatttgcat tcggattcac ttttttgtat gcctctacaa
     3301 gtttctccat taccggagct acagaagaag atcctcctac aactacgctt ccttccggct
     3361 tagttcctgc atatgcctga acatcggata ccggtatgta acctgcttct tctacaactt
     3421 tctgaccttc cgtactcatg ataaaactca taaaatcttt tgccgcttca ttgtccagat
     3481 ctttctttac tgctacatta aacggtctgg atactttgta acttccgttt tcaatatttt
     3541 ctgccgttgc ttcggctccg tcgatcttaa gtgcttttac agcgtcctca tcaagagatc
     3601 caagagaaat atatccgatc gcatattcat ttccttccac tgttgtcatc ataaccgaag
     3661 tattgtttgt gatctgcgct tccgttgtcg tcatgtcgac tttttctccg tcaacttctt
     3721 cctgaatgcc gaacaattcg ataaacgcgc ctcttgttcc cgaaccatcc tcacgggaaa
     3781 caactgtaat gtcatttgcg ctgtcccagt ctcctgcata ttttgcatct ccgcctttat
     3841 ccgcatcttt tgcatcatcg cttcctgatc cacagcctgt catagctgct gccaacattc
     3901 ctaccataga tacaactgcg ataaactttt tcattttcat agtcttacaa tctccttttt
     3961 gcttccgctt tttcattttt ttgttttctt ttattctttt ttatttcttt ctattcagcg
     4021 aaaacaccta aaaccaactt cacatttcgc tgttttcttt tgtgctcctt tgcacattga
     4081 ctatcataaa taacaaatgt aaaatgtaaa atcagcgcta tgttaaatct atgtaaaatg
     4141 aaatttattc taaagcagcc gccgggatct tgaacaattc cggactttgg cttgccggct
     4201 atacaaaaag cgcccacaca aaaatcctgt tttttcaaac aatgattttt gtgtgggcgc
     4261 tttttccatg gagtataggg gagtcgaacc cctggcctca acaatgcgaa tgttgcgcgc
     4321 tcccaactgc gctaataccc cttatggacc tggcgggaat cgaacccgcg tccaaaaacc
     4381 tatcccatgt acttctacta tcatagtcag ttctttttca ttccctctgc cgcacgggaa
     4441 ctaacaccct tacggtttca gtagcttcat gatacgccta tcggctcaaa gctttgccaa
     4501 tatcgtttct cacatcgttg atgccggatt tccgtggtgt gagtgccaca gagccgacat
     4561 gcggcaatta ggccgcagct aattcgaaat tatcgttagc gtttaatttt aagtttgcga
     4621 tttaacgcat cagcctgcgg atagcttctc catcttcaag atccctgtcg aaaccagtac
     4681 aagccctcgt ctgtcaggtt gtacagaaga tgtaattttt acatcattta taatatagaa
     4741 tatactgctt caaaaatcaa gggggtatag cgcattattt cttttcttcc gcttattctt
     4801 ctttttattt tatctttaaa actacagttc ttctccattc gaccggatta cgttcttgta
     4861 ccagtcaaaa gacttcttct tatacctgtt tccgcttcca tttccttcgt tatcaatatc
     4921 gacataaata aatccatatc tttttttcat ctcgcctgtt gtaaacgata caagatcaat
     4981 gcatccccac ggggtatatc ctataagatc tactccgtca agttcaaccg cttttttcat
     5041 ttcggtaata tgttctctta aatatgcaat tctataatcg tcatcacatg ttttatcttc
     5101 ttttaacaca tcgatcgcgc cgaatccatt ttctacaata aacagaggtg cttcgtaacg
     5161 ttcataaaga gtaaccagag catatcttaa tccgacagga tctatctgcc accccccagt
     5221 cacttgcctt cacatacggg tttggtacag aatacggaga acttccgtcc agagattctg
     5281 tcgtatcttt ttttgcatct gctttcaccg cattcgtcat ataataacta aatcccagat
     5341 agtcgcattt tccttccgcc aaaatcttct cgtcttcagg aagcatctgt ggttttgttc
     5401 cttccctctc ccactccttt tttgcataag ccggataatg ccctctcata tgtacgtctg
     5461 aaaaataaaa tctttcatgc atacattctg ccgcagtcat aacatcttct ggattgcagg
     5521 aatacggata aaacggaaca aaagaacaca tacatccgat ctgaaactcc ggattgatct
     5581 cgtgaccttt tttcacaacg atcgcacttg caagcagctc atgatg
//
LOCUS       DBMDXF010000121         5600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3609_length_5600_cov_2.055828, whole genome shotgun sequence.
ACCESSION   DBMDXF010000121 DBMDXF010000000
VERSION     DBMDXF010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3609_length_5600_cov_2.055828"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="ACZPD4_05870"
     CDS             complement(<1..384)
                     /locus_tag="ACZPD4_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR1378623.1"
                     /translation="MKTYKKNNTDSIGSLSLSDSILSGATITSQPAGYTFPHHLHSTI
                     EIYRIISGECLMDVQSETLHCTKGDFIMILPNIVHSFYLNKKSDCTFKHVHFDPNMFS
                     NIILENEGVYPITLMHAVLFSSLFYY"
     gene            609..2945
                     /gene="feoB"
                     /locus_tag="ACZPD4_05875"
     CDS             609..2945
                     /gene="feoB"
                     /locus_tag="ACZPD4_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1378624.1"
                     /translation="MTLKELGIGQKGIILSVGGSGALRQHFLDMGLIRGAEVTVVKYA
                     PMGDPIELKIHGYELTIRLDDAEKIEVKEIKSEEKEKERRNKVKRSAHPGYGEGGKYH
                     KKETENPLKDDETLTFALVGNQNCGKTTLFNQLTGASQHVGNFPGVTVDRKDGVIKGY
                     KNTLITDLPGIYSMSPYSSEEIVTREFLLNEKPKGIINIVDATNMERNLYLTMQLMEL
                     DMPMVVALNMMDELRENGGSVHVNEMEEFLGVPVVPISAAKGEGIEELVKHAVHVAKY
                     QESPQKQDFCADTESGIHRGLHAVMHLIDDHAQKAGIPLRFAASKIMEGDKKITEKLD
                     LSRNEKEMLEHIVVQTEEESGFDRASAVADMRFSYITEVCETAVVKPHESKERLRSKR
                     IDRILTGKYTGIPAFIAIMGIVFWLTFNVIGAGLQSLLETGIGYLTEITDQAMTSAGV
                     NTAVHSLVIDGIFNGVGGVLSFLPIIVTLFFFLSLLEDSGYMARVAFIMDKLLRKLGL
                     SGRSIVPMLVGFGCTVPGVMASRTLPSERDRKMTILLTPFMSCTAKLPIYAFFSAVFF
                     PDYAALVMIGLYVLGIVMGIIMALLFKGTMFKGEPVPFVMELPNYRLPGMKNVGQLLW
                     EKAKDFLQRAFTVIFAATIVIWFLQSFDTSLNMVTDSKDSILAMIAGALSPVFAPLGF
                     GDWRISTALVTGFMAKESVVSTLTVLFGTTEALLGTITAAGAAALLVFCLLYTPCVAA
                     VASIKRELGGKWAFFVVIGQCVIAWLAAFAVHTVAMLF"
     gene            complement(2942..3700)
                     /locus_tag="ACZPD4_05880"
     CDS             complement(2942..3700)
                     /locus_tag="ACZPD4_05880"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0106029 - tRNA pseudouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR1378625.1"
                     /translation="MPNIKLTLQYDGTRYLGWTRPAADGYAKTISYRISSVLQKMTGE
                     DITLFAGAKTAPGVHALCQTVNFHTESPFSDTYFLSKLNEYLPKDIHILSSEIVSDRF
                     RSDLNAVSRTYLYRICTAPVQNIFTRAYTANIPEIISESEVAAIRKAADSLVGVHDFR
                     SLSGVKKKKGTVKEIFDISVSHSKETENDSFSLLTIKICANDFLYLMPEHIIEFLIKK
                     GVDKEVRCNPAGLLLHSITYPDSCSVPSAGSKQV"
     gene            3934..4410
                     /locus_tag="ACZPD4_05885"
     CDS             3934..4410
                     /locus_tag="ACZPD4_05885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR1378626.1"
                     /translation="MIEEKRVEDSRVETVHIIRPNHLNAAGRLFGGMLMQWLDEVAGM
                     VAKRHTRANVITASVDNLRFIHGAYKGEMVVIVGKVTYVGTTSMEVRVDTYVEHMEDG
                     MRRPINRAYFTMVALDEDDKPKQVPRLILETEAQRAEWKAAEKRREMRMRRKEEGF"
     gene            complement(4424..4711)
                     /locus_tag="ACZPD4_05890"
     CDS             complement(4424..4711)
                     /locus_tag="ACZPD4_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378627.1"
                     /translation="MNDWMNNPAMKNIDPIKLELIKTAASKTSGKSGKELAPIMMALI
                     SGANKQGIRFTPEEMTLILDILKDGKSKEEREQIDRTINMTSSLFRKHNGK"
     gene            complement(4750..5259)
                     /locus_tag="ACZPD4_05895"
     CDS             complement(4750..5259)
                     /locus_tag="ACZPD4_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378628.1"
                     /translation="MDQDQTERMTPFDELVTSPTLQIMKLLIPYTPFSGRRMLASFIK
                     FIELQRTIRLFGQRSLADPFSTGNSDQNISSPFDLIDSFRPYLNKKDAEMLDTLIQLK
                     EMMSFMEMMQPQDGQGNDSFNPADLFSGIFPADLQEQFHMYSDLFSSVSQNQEHDETD
                     AQQKKENEL"
     gene            5393..5521
                     /locus_tag="ACZPD4_05900"
     CDS             5393..5521
                     /locus_tag="ACZPD4_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378629.1"
                     /translation="MSRKLIIDGNAVYEIDEQCMLRRKLEENAEATKGSSENKKGT"
BASE COUNT         1643 a          939 c         1438 g         1580 t
ORIGIN      
        1 gtaatagaaa agcgaagaaa ataaaactgc atgcattaat gtgatcggat aaacgccttc
       61 gttttctaaa ataatatttg aaaacatgtt gggatcaaag tggacgtgtt taaacgtaca
      121 gtcagatttt ttgttcagat aaaaagagtg aacgatattc ggcagtatca taataaaatc
      181 gccttttgtg cagtgaagtg tttccgactg gacatccata agacattcgc ctgatatgat
      241 acgataaatc tctattgtag agtgcagatg atgcggaaat gtgtatcctg ccggctgtga
      301 tgtgatagtt gcgcctgata agatcgaatc ggacaaagac agtgatccga tcgaatcggt
      361 attgtttttt ttgtatgttt tcatgaaacg ttcctcttga tcattgcata aacttatatg
      421 ttggttatat acggttatat atagattaat tataccagaa gtttattaaa aagtgacaaa
      481 aaatataaaa atatgtagat aaaatgagaa cttattccga attatataat ttgaaaaaaa
      541 tctcgcaatt taaggttttt ctgttataat gttagttgac gcttactaaa agatgaggag
      601 tgaagattat gacattgaaa gaacttggca tcggacagaa aggaattatc ttatctgtcg
      661 gaggaagcgg agcactcaga caacattttc tggatatggg attgatacgg ggcgcagaag
      721 tgacagtcgt aaaatatgcg cctatgggcg atccgatcga gttgaaaata catgggtatg
      781 agctgacgat ccgtttggat gatgcagaga aaatagaagt aaaagaaata aagtcagaag
      841 aaaaagagaa agaaagacgg aacaaggtga aacgttctgc acatcccgga tacggtgaag
      901 gcggaaagta ccataagaaa gaaacagaaa atccgcttaa ggatgatgag acgttgacgt
      961 ttgccctggt aggaaaccaa aattgcggaa agacgacgtt gtttaatcag ttgacagggg
     1021 cttcgcagca tgtcggaaat tttccgggcg ttaccgtaga ccgcaaggac ggcgtgatca
     1081 aagggtacaa aaatacatta atcacagacc ttccgggaat ttattctatg tctccttaca
     1141 gcagtgagga gattgttaca cgagaatttt tgttaaatga aaagccgaaa ggaatcatta
     1201 atatcgtcga tgcgacgaat atggagagga atctctattt aacgatgcag ttgatggagt
     1261 tagatatgcc tatggtcgtt gcattgaaca tgatggatga attgagggaa aacgggggaa
     1321 gcgttcatgt aaatgaaatg gaagagtttc tcggtgttcc ggttgtaccg atctccgctg
     1381 ccaaaggaga gggaatcgaa gagcttgtga aacatgcggt gcatgtggca aagtatcagg
     1441 aaagtcctca aaaacaggat ttttgtgcgg atacggaaag cgggatccac agaggacttc
     1501 atgcagtcat gcatttgatc gatgatcacg cacaaaaagc cggaatccct cttcggtttg
     1561 cggcaagcaa gatcatggaa ggcgataaaa agattaccga gaaattggat ttaagccgga
     1621 atgaaaaaga aatgctcgag cacatcgttg ttcagacgga agaggagagc ggttttgaca
     1681 gagcttcagc cgttgccgat atgcgattct cttacataac ggaagtgtgt gagacggcgg
     1741 ttgtaaaacc gcatgagagt aaagagcgtc tcaggagtaa acggatcgac cgcatactga
     1801 cgggaaaata tacgggaatc ccggccttta tcgcgattat gggaattgta ttctggctga
     1861 catttaacgt gatcggagcg ggattacagt cgcttttgga aacgggaatc ggatacttga
     1921 cagaaatcac agatcaggcg atgacatccg cgggagtcaa tacggcggtt cattccctcg
     1981 ttattgacgg gatttttaac ggtgtgggag gagtccttag tttcctgccg atcatcgtta
     2041 cattgttttt cttcttatct ttgctggaag acagcggata tatggcaaga gtagctttta
     2101 ttatggataa gctgcttcgt aagcttggat tgtcgggaag aagtatcgtg ccgatgctgg
     2161 tcgggttcgg atgtaccgtt cccggggtga tggcgagcag aacgcttccg tcggaaagag
     2221 acaggaaaat gacgattttg ctgactccgt ttatgagctg tacggcaaag cttccgatct
     2281 atgcattctt ttcggctgta ttttttccgg actatgccgc gcttgtgatg atcggattgt
     2341 atgttcttgg tattgttatg ggaattatca tggccctttt gtttaaagga acgatgttta
     2401 aaggggagcc tgttccgttt gttatggaac ttccgaatta ccgtcttccg ggaatgaaaa
     2461 atgtcggaca gttattgtgg gaaaaagcga aagattttct tcagagagct tttacggtta
     2521 ttttcgcggc gacgatcgtg atctggttct tgcaaagctt tgatacaagc ctgaatatgg
     2581 tgacagattc aaaggacagt attttggcaa tgatcgcggg agctctttca ccggttttcg
     2641 caccgcttgg gtttggggac tggcgtatat cgacggcgct tgtaaccgga tttatggcaa
     2701 aagaaagtgt tgtctcgacg ctgacggtgt tgttcggcac tacggaagcg cttcttggaa
     2761 cgatcacggc ggcaggagct gcggcgcttc tcgtattttg cctgttgtat actccctgtg
     2821 tggctgcggt tgcatcgatc aagcgggagc ttggcggaaa atgggcgttt tttgttgtca
     2881 tcggccaatg tgtgatcgca tggcttgcgg cattcgcagt tcatacagtt gcgatgcttt
     2941 tttaaacctg cttggacccg gcagagggaa cggaacatga atcgggatag gtaatggagt
     3001 ggaggagaag tcctgccgga ttgcagcgta cttctttatc cactcccttt tttatgagaa
     3061 attcaatgat gtgctccggc atcaggtaca gaaaatcatt tgcacagatt ttgatcgtaa
     3121 gaagcgaaaa gctgtcgttt tcggtttctt tggagtggga aacggaaatg tcaaatattt
     3181 ctttgactgt tccttttttc ttttttactc ctgaaaggga acgaaaatca tggactccga
     3241 cgagagaatc agccgccttt cttatggcgg caacttctga ttccgagatg atttccggta
     3301 tatttgccgt gtaggctctt gtgaatatgt tttggacagg ggcggtacag atgcggtaca
     3361 aataagttct ggagactgcg tttaaatctg atcggaaacg atcagaaaca atttcggaac
     3421 ttaagatgtg aatatctttc ggaagatatt cgttaagctt tgataaaaaa tatgtatcgg
     3481 aaaaaggaga ctctgtgtga aagttgactg tctgacagag tgcgtggact ccgggcgctg
     3541 ttttagcgcc ggcaaaaaga gtgatgtctt caccggtcat tttctgtaaa acagaagaaa
     3601 tgcgatatga aatcgttttc gcgtatccgt ctgcggcagg acgtgtccat cctaaatagc
     3661 gtgttccgtc gtattgaagg gttagtttta tgttgggcat aaggtactcc tttgtgcggt
     3721 tatttgtata tcatatgtct gtataatagt gtatcgggta tcggatgtca aacggaatat
     3781 acaggggtga ttttctaaaa tgattatgat atactggcta cgtgaaatca gagtaaataa
     3841 gaaaaacagt ttaagattca ttttgttgca caatgaaaaa agcaataaaa aaatataaaa
     3901 gaaaacaaaa aagtgtaaaa gaaggagaaa aggatgatag aagaaaaaag agtagaagat
     3961 tccagagtgg agacggttca cattatcaga ccaaatcatc tcaatgcagc cggccggctt
     4021 ttcggcggga tgttaatgca gtggcttgat gaagtagcgg gaatggtggc aaagagacat
     4081 acgagagcga acgtcataac tgcgtcagta gataatcttc ggtttatcca tggcgcatat
     4141 aaaggcgaga tggttgttat agtcggaaaa gtaacatatg tcggcacgac atctatggaa
     4201 gtgcgtgtag atacttatgt ggaacatatg gaagatggga tgcgccgtcc catcaaccgc
     4261 gcgtatttta cgatggttgc gttagatgaa gacgacaaac caaagcaagt gccccgtctg
     4321 attttagaga cagaagcgca gcgcgcggaa tggaaagcgg cagagaaacg gcgtgagatg
     4381 aggatgagac gaaaagaaga aggtttttaa taaaatatag agtctacttt ccgttgtgtt
     4441 ttcggaagag cgaggaggtc atattgatcg ttcgatcgat ctgctcccgc tcttctttgc
     4501 tttttccgtc ttttaaaatg tcaaggatca aggtcatttc ctccggtgta aaacggattc
     4561 cctgtttgtt agctccgctg atgagagcca tcataatagg agccaactct tttccggatt
     4621 ttccggaagt tttggaggcg gctgttttga tcagttctaa ttttataggg tcgatatttt
     4681 tcattgcggg attattcatc cagtcgttca tattggtaat tcctttctat aaatgatttt
     4741 aaatattttt taaagttcgt tttctttctt ttgctgtgca tctgtctcat cgtgctcttg
     4801 attttgcgaa acggatgaaa acaggtcgct gtacatatgg aactgttctt gaagatctgc
     4861 gggaaatatg cccgagaaca ggtcggcagg attaaaagaa tcgtttccct gaccgtcttg
     4921 cggctgcatc atttccataa aagacatcat ttcttttaat tgaatcaaag tatcgagcat
     4981 ttcggcatct tttttgttca gataaggccg gaaactgtca ataagatcga aaggagaact
     5041 tatgttttga tcgctgtttc cggtggaaaa aggatctgcc aggctgcgtt gtccgaaaag
     5101 ccggatcgtt ctttgaagtt caataaactt gataaatgag gcgagcatcc ggcgtccgga
     5161 aaatggggtg taagggatga gaagcttcat gatctgaagt gtaggcgatg tgacaagttc
     5221 atcaaaaggc gtcattcgtt ccgtttggtc ttgatccatt tccaaaatgc tccactgtat
     5281 agagttgtaa atatatatgt gactttgtat agaaatatgt attttgggaa tgttactttt
     5341 tagaaaatat ttcataaaat agtaaagaat gggtacaaga aaggaaatga atatgtcaag
     5401 aaaattaatt atcgatggga atgcagtgta tgagattgat gaacagtgta tgttgagacg
     5461 aaaattggaa gaaaatgccg aggcgacaaa aggcagcagt gagaacaaaa aagggacatg
     5521 agaaagcgct tgatagagtt taggaaactc ttgcacccaa gctgaaccag aaaagtccga
     5581 atccttgaca acatacttgg
//
LOCUS       DBMDXF010000122         5503 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3677_length_5503_cov_1.573491, whole genome shotgun sequence.
ACCESSION   DBMDXF010000122 DBMDXF010000000
VERSION     DBMDXF010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_5503_cov_1.573491"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(43..2286)
                     /locus_tag="ACZPD4_05905"
     CDS             complement(43..2286)
                     /locus_tag="ACZPD4_05905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HIR1378630.1"
                     /translation="MNSESQIIRTEYSDLMKKSYIDYAMSVIISRALPDVRDGLKPVQ
                     RRTLYDMYELGIRYDKPYRKCARIVGDTMGKYHPHGDSSIYESLVVMAQEFKKGMVLV
                     DGHGNFGSIEGDGAAAMRYTEARLTKFTQEVCLADLDKDVVDFGPNFDETEKEPLVLP
                     MRVPNLLVNGAEGIAVGMATSIPPHNLGEVIDAVKAYIKNDSITTKQLMKYIKGPDFP
                     TGGIVVNKDDLINIYETGQGKIKVRGKVETEELKGGKKQLVISEIPYTMIGAGIGKFL
                     NDVAGLVETKKTTDITDISNQSSKEGIRIVLELKKGADVENLKNMLYKKTRLEDTFGV
                     NMLAVADGRPETLGLKKIIEHHVDFRFELATRKYKTLLAKEQEKSEVQEGLIKACDVI
                     DLIIEILRGSKSVKDAKACLVNGVTDNIKFKSSISKKMAVLLRFTERQAAAILEMRLY
                     RLIGLEVQALIAEHEQTVKNIARYEDILNNYDSMAQVIVEELESFKEEYAKARRTVIE
                     NAEEAVFEEKKIEEQEVVFLMDRFGYARIVDVAVYERNKEAADSENKYIVHCMNTGKI
                     CIFTNSGKMHQVKVIDLPYGKFRDKGVPIDNVSNYSSSDEQIVSVYDSEQMRFAMLLF
                     STAQGMVKKVSGSEFQVAKRTILATKLQEEDSLVDVRVVNENKHVVLQTKNGYFLRFP
                     ATEVPIKKKAAVGVRGIRLQKKDELEKVYLFEEGTETKILYNEKEVSLNRLKTAKRDG
                     AGTKARK"
     gene            complement(2378..4300)
                     /locus_tag="ACZPD4_05910"
     CDS             complement(2378..4300)
                     /locus_tag="ACZPD4_05910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit B"
                     /protein_id="HIR1378631.1"
                     /translation="MAGNNTYDAGSISVLEGLEAVRKRPGMYIGSVSTKGLNHLIYEI
                     VDNSVDEHLAGFCTEIRVVLEKDGTATVTDNGRGIPVDMHQKGISAARLVFTTLHAGG
                     KFDDSAYKTSGGLHGVGSSVVNALSTYMDVKISRDGAVYHDRYERGVPVIELENGLLP
                     KIGKTKKTGTTINFLPDDTIFEKTKFRAEEVKSRLHETAYLNPELTIIFEDKRGDQTE
                     HIEYHEPEGIIGFIRDLNKKKEALHEPVYFKGEADGIEVEAAFQYVNEFHENVLGFCN
                     NIYNAEGGTHLTGFKTTFTTVMNQYAREIGILKEKDTNFTGADIRNGMTAIISIKHPD
                     PRFEGQTKTKLDNPDAAKAVGKVAGDEIVLYFDRNLDVLKTVLSSAEKAAKIRKTEEK
                     AKTNLLTKQKYSFDSNGKLANCESRDPKKCEIFIVEGDSAGGSAKTARNRNFQAILPI
                     RGKILNVEKASIDKVLANAEIKTMINAFGCGFSEGYGNDFDITKLRYDKIIIMADADV
                     DGAHISTLLLTLFYRFMPELIYEGHVYIAMPPLYKAMPKSGEEEYLYDDKALEKYRRT
                     HKGPFTLQRYKGLGEMDAEQLWETTLDPKRRLLKLVEIEDARMASGVTEMLMGTEVPP
                     RRAFIYENATEAELDI"
     gene            complement(4482..>5503)
                     /gene="spoIIP"
                     /locus_tag="ACZPD4_05915"
     CDS             complement(4482..>5503)
                     /gene="spoIIP"
                     /locus_tag="ACZPD4_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HIR1378632.1"
                     /translation="NRSPQKTVHEFFIEQSYLFLPTAGYAMEHRREGFTAEDQETVAQ
                     ILEAQANDENCIDENGNLISKESPETAQNVQPSGETSLSSVDMSIERLRDFDYLLSNF
                     YTVDSSTMIGPEQLNADDLLGRSMKINKKEDGPKVLIFHTHSQEEFVDSIPGDPATSI
                     IGIGEYLTELLNKRGIKTIHDTGVYDIINGQLDRSNAYENAEASVRPILEANPTIEVA
                     IDLHRDGVAEGTHLVTEVNGKPTAKIMYFNGLSRSRTNGDIDYLYNPYIQDNLAFSLQ
                     MQLASERMYPGFARHIYLRAYRYNLHLLPKSLLIEAGAQTNTVEEMKNAMEVVADTLC
                     EVILE"
BASE COUNT         1351 a         1398 c          905 g         1849 t
ORIGIN      
        1 ttgatcggga tgtaacatat ttttgacaaa cctacatctt ctctattttc ttgcttttgt
       61 tcctgctcca tcacgtttcg ccgttttcaa tcgatttaag gagacctctt tttcattata
      121 tagaatcttt gtttccgttc cttcttcaaa aagatatacc ttttccagtt catctttttt
      181 ctgaagacgg atacctctga caccgactgc agcttttttc tttataggaa cttccgtggc
      241 cgggaatctc aaaaagtacc cgttctttgt ctgaagaacg acatgcttat tttcattaac
      301 aacccgaaca tcgaccagag aatcttcttc ctgaagtttt gtagccagaa ttgttcgttt
      361 tgccacctga aattcacttc cgctgacttt ttttaccatt ccctgcgccg ttgaaaacaa
      421 aagcatggca aatctcatct gctcggaatc gtataccgag acaatctgtt catcagaact
      481 ggagtaattg ctgacattgt cgatcggaac tcctttatcc ctgaattttc cataaggaag
      541 atcaattact ttcacctggt gcattttccc gctattggta aaaatacata ttttccccgt
      601 gttcatacaa tggacaatat acttattctc actatccgcc gcttccttat ttctttcgta
      661 aacggcaacg tctactattc ttgcatatcc gaaccgatcc atcaaaaata cgacttcctg
      721 ttcttcgatc ttcttctctt caaaaacggc ttcttcagca ttttcaatga ctgtacggcg
      781 cgcttttgca tattcttctt taaacgattc aagttcttcc acaataactt gcgccataga
      841 atcataatta ttgagaatat cttcataacg cgcaatattc ttcaccgtct gttcatgttc
      901 cgcgatcaaa gcctgtactt ccagaccgat gaggcgatat aagcgcatct cgaggatggc
      961 agcagcctga cgttctgtaa agcgtaaaag caccgccatc tttttggaaa tagaagattt
     1021 aaacttaata ttatctgtta cgccatttac aagacatgct tttgcatctt ttacagattt
     1081 actgcctcga aggatttcaa taataagatc gatcacatca catgctttga tcaacccctc
     1141 ctgcacctca cttttttcct gttcttttgc aagaagtgtt ttgtattttc tcgtcgccag
     1201 ttcaaatcga aaatctacat gatgctcaat gatcttctta agtccgagtg tttccggtct
     1261 tccgtctgca actgcaagca tattcacgcc gaatgtgtct tcaaggcgcg tctttttata
     1321 aagcatattt tttagatttt ctacatctgc gccttttttt aattccaaca caatccgaat
     1381 tccttctttg gaagattggt ttgaaatatc tgtgatatct gttgttttct ttgtctctac
     1441 cagaccggct acatcattta aaaacttccc tattcccgct ccgatcatcg tgtatgggat
     1501 ctcactgatc acaagctgct ttttgccgcc ttttaattct tctgtctcta cttttccccg
     1561 gactttgatc tttccctgtc ccgtttcata tatattgatc agatcatctt tattaacaac
     1621 aattccgcct gtcggaaaat caggcccttt tatgtatttc atcagttgtt ttgtagtgat
     1681 cgaatcgttt ttaatatatg cctttaccgc atcgatcact tctccgagat tatgaggcgg
     1741 tatacttgtc gccataccta ccgcaattcc ttccgctccg tttacgagga ggtttggcac
     1801 acgcatcgga agtacaagcg gttctttttc tgtttcgtca aaattcggtc cgaaatctac
     1861 cacatcttta tctagatctg caaggcagac ttcctgcgtg aatttcgtca gtcttgcctc
     1921 agtataacgc attgccgcag cgccgtcacc ttctatcgaa ccgaagtttc cgtgaccgtc
     1981 tacaagtacc attccttttt taaactcctg agccataaca acaagagatt cgtagatcga
     2041 actgtcgccg tgaggatgat atttacccat cgtatctccg acaatacgcg cgcattttct
     2101 gtacggctta tcatagcgga ttcccagttc gtacatatca taaagcgtac ggcgctgaac
     2161 cggcttcagt ccgtctctta catccggaag tgccctggaa ataattacgc tcatcgcata
     2221 atcaatatat gattttttca taagatccga atattccgtt cttatgatct gtgattcact
     2281 gttcatttct cgtctccttc atttcatttt gctcatatga cacaaagcaa atccattttc
     2341 tgacacaaat cacgtcgact ctgtttcgtc atacagatta tatatcaagt tccgcctctg
     2401 tcgcattttc atagatgaat gcccgccgcg gaggcacttc cgttcccata agcatctcag
     2461 tcacaccgga agccattctc gcatcttcaa tctcaacaag ctttaaaaga cgtcttttag
     2521 gatcaagtgt tgtttcccac aactgttctg catccatctc tccaagccct ttatacctct
     2581 gaagagtaaa cggtcctttg tgtgtccttc gatatttttc aagcgcctta tcatcgtaaa
     2641 gatattcctc ttctccgctt ttcggcattg ccttataaag cggcggcatt gcaatataca
     2701 cgtgcccctc ataaatcagt tccggcataa accgatagaa cagcgtcagc aaaagtgttg
     2761 aaatatgagc tccgtctaca tctgcatctg ccataattat gatcttatcg tatcgaagct
     2821 ttgtaatatc gaagtcattg ccgtatcctt cggaaaatcc acatccgaac gcattgatca
     2881 tcgtttttat ttctgcattc gcaaggactt tatcaatact tgctttctcc acgtttaata
     2941 ttttcccgcg aataggaaga atcgcctgaa agtttctgtt tctggccgtt tttgccgaac
     3001 ctcccgccga atctccctca acaataaata tctcgcactt tttcggatct ctgctctcgc
     3061 agtttgcgag ttttccgtta ctgtcaaacg aatatttttg cttcgtcaaa agattcgttt
     3121 tcgccttttc ttctgtctta cggattttcg ccgccttttc cgcacttgac aataccgttt
     3181 ttaaaacgtc caggttacgg tcaaaataaa gaacaatttc atctcccgca actttaccga
     3241 ccgcttttgc cgcgtcggga ttgtccagtt ttgtctttgt ctgcccttca aatctaggat
     3301 ccggatgttt gatcgaaata atcgctgtca tcccgtttcg gatatcagct cccgtaaagt
     3361 tcgtatcctt ctcttttaaa ataccgattt ctcttgcata ctgattcata accgtcgtaa
     3421 atgtcgtttt aaatccggtt aaatgcgttc cgccctcggc attgtaaata ttgttgcaga
     3481 aaccgagcac attctcatga aattcattaa catactgaaa tgccgcctcc acttcaatac
     3541 cgtctgcttc ccctttaaaa taaacaggct catgaagcgc ctcttttttc ttattcagat
     3601 ctctgataaa cccgatgatt ccctccggtt catgatattc gatatgctcg gtctgatctc
     3661 ctcttttatc ttcgaatatt atcgtcagtt ccgggttaag atatgctgtt tcatgcagcc
     3721 tgctttttac ttcctccgcc cggaacttcg tcttttcaaa aatcgtatca tcaggcagaa
     3781 aattaattgt tgttcctgtc ttttttgtct ttccgatctt aggaagaagt ccgttttcca
     3841 gttcaatgac aggaactccg cgctcatatc tgtcatggta taccgcccca tcacggctga
     3901 tcttaacatc catatatgta gaaagtgcat ttacaacaga agaaccgacg ccatgaagac
     3961 ctccgcttgt cttatacgcc gaatcgtcaa attttccccc tgcatgaagt gtggtaaaca
     4021 caagccttgc cgcagaaatt cctttttgat gcatatcaac cggaattccc ctcccgttat
     4081 cggttactgt tgccgttccg tctttttcta aaacaacacg gatttccgta caaaatcctg
     4141 ccagatgttc atccacggaa ttgtccacaa tttcataaat aagatgattc agaccttttg
     4201 tcgatacact tccgatatac attcccgggc gttttcgtac cgcctctaat ccttccaata
     4261 cagaaatact ccctgcatca tatgtattat ttccagccat ttaccttctc ctttaccgga
     4321 tcattccttc tatttttctc gacttctttt cttatgccgc actaattttg tttcacgcta
     4381 taacgagatt gtaccctcag atgccggatg ttgttccttt catctgaggg tattattttc
     4441 tatgatacca cccgttttcg atcttgtaaa tagaatcctt gtcattccag tataacttca
     4501 cacagcgtat ctgccacgac ctccatcgca tttttcattt cttctacagt atttgtctgc
     4561 gcaccggctt caatcaacag agacttcggc agcagatgca gattatatct atatgcccgc
     4621 agataaatat gtcttgcaaa cccgggatac attcgttctg acgcaagctg catctgaaga
     4681 gaaaacgcca gattatcctg tatgtacgga ttataaagat aatcgatatc tccgtttgtg
     4741 cgagatcggc tcaatccatt aaaatacatg atctttgcag tcggctttcc gtttacttct
     4801 gtgactaagt gcgttccctc ggcaacgcca tccctatgaa ggtcgattgc cacctcgatc
     4861 gttgggtttg cctccagaat cggtctgaca gacgcttctg cgttttcata tgcattactc
     4921 ctgtcgagct gtccgttaat aatatcatat acccctgtgt catggattgt tttgattccc
     4981 cgcttattta aaagctctgt cagatattcc cctattccga taatacttgt ggcaggatct
     5041 cccggaattg aatcgacaaa ttcctcctgt gaatgagtat gaaatatcaa tactttcgga
     5101 ccatcttcct ttttattgat cttcatactc ctcccaagaa ggtcatctgc attcaactgt
     5161 tcaggaccaa tcatcgtaga gctgtctacc gtataaaaat tactaagcaa ataatcaaaa
     5221 tctcttaacc tctcaataga catatcgaca gaagaaagtg acgtttctcc cgacggctgc
     5281 acattttgcg ccgtttcagg agactcttta ctaataagat ttccgttttc atcgatacag
     5341 ttctcatcgt ttgcctgcgc ctctaaaatt tgagcaacag tttcttgatc ttctgccgta
     5401 aatccctctc tcctatgctc catcgcatac cctgctgtcg gaagaaacag ataagattgt
     5461 tctataaaaa actcgtgaac tgttttttgc gggcttctgt tca
//
LOCUS       DBMDXF010000123         5477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3696_length_5477_cov_1.664062, whole genome shotgun sequence.
ACCESSION   DBMDXF010000123 DBMDXF010000000
VERSION     DBMDXF010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3696_length_5477_cov_1.664062"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="ACZPD4_05920"
     CDS             complement(<1..1261)
                     /locus_tag="ACZPD4_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1378633.1"
                     /translation="MGKYETLAKDIVKNVGGKENVTGLVHCITRLRFTLKDESIAKDD
                     VLKKMDGVVTVMKSGGQYQVVIGNHVPEVFEDVMALLDLNENKASAETKKSGKLLDRA
                     IDVVSGIFQPILGIMAACGMLKGLNTLFVTLGLYSADCGGYMIINAAGDALFTFLPLF
                     LGYTAAKKFQLKPMLGLAIGAAMCYPGIQLSALSAGAEAVTTVLEGTLFQSPVYIDFF
                     GIPVISVDYTGTVIPVILTVWFASKCEKLFNRFVPDLVKFFFVPMLTLLVTLPVAMIF
                     LGPVATFGSTLISEFTLMIREFSPLLAGAVVGFTWQILVIFGMHWGFIPVYINNVQTL
                     GYDNVMMPFFACTFATSAVVLAMFFKTKDKKIKEMAIPNFISGIFGVTEPAIYGMLLP
                     LKKPFIISCIAGGIGGAFYGHFNFRKFI"
     gene            complement(1403..2278)
                     /locus_tag="ACZPD4_05925"
     CDS             complement(1403..2278)
                     /locus_tag="ACZPD4_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1378634.1"
                     /translation="MLLEKLQEHKNFTAHEKDVAEYILSHLDQISELSVGELAKASYT
                     SKATVVRLSQKLGLNGFQDLKITLTAEINQKKRIDQLLANEPVTAESTYDDIVKTLPA
                     LYDKAVTNTRLSLDKNSMKRISQVLQKAECIDIYGTGISYILAQSAAFKISTLGLECS
                     AYESINSHYLAARKHKKNIAFLISLTGSNHTVICMAKYLREATETYVVGIAGPYHTEL
                     KKWCHEIVEIPNRDALLSLDVITSFSGATYILDIFFALLLSKRYEEHARSSMEMLNHL
                     SLLWDETYHTKEKNE"
     gene            complement(2250..3161)
                     /locus_tag="ACZPD4_05930"
     CDS             complement(2250..3161)
                     /locus_tag="ACZPD4_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1378635.1"
                     /translation="MEIKIRELCKSYGKKKALQNFDATFENGIYGILGPNGAGKSTLM
                     NILTDNLLRDSGRILCGGKDILKWGAAYRKSLGYMPQQQQLYETFTVTEFLAYMGTLK
                     GMKKKEIRARMELLFPLLNLDKVKRKKIKELSGGMKQRVLLLQALLNDPKILILDEPT
                     AGLDPKERIRIRNLISDLSQDRIVLIATHVVSDIEFISKEILLMKNGRLIDKASPEQL
                     QAKIQGKVFEMSVGQQELEEVKEKFEISNLFRKDGQIIVRVVTQKRPEGYDEIREAAP
                     TLEDVYLYEFEVKGAMHNNAVRKIAGT"
     gene            complement(3167..5395)
                     /locus_tag="ACZPD4_05935"
     CDS             complement(3167..5395)
                     /locus_tag="ACZPD4_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1378636.1"
                     /translation="MKSVMYEWKKFRAFRYFWFLCSVFFLFNIWTLSEKMKMEMYDAG
                     AVKQIYADLKAQPEEKRKEWIEKLPEKKVPVYTGNEYAEEMLYNKIFQEYRQVNRYDK
                     YLEEIKEKSASMSSAIFSNEGTFAYRNAQITPDAYEKLKGLKLKSDVSDGVIFATEAE
                     FTDLCIVFMLAVTVYFLVLDEKKNKLYPLLRSCYRGRGSVIGAKLTVAACVTALLVLL
                     FYGVDYLYAWQKYGFGDLSRPIQSVTTMYESPYMMSVGMFLFLYLIVKMAVCYVIILG
                     MIWIAQKSETPSGAMIGIGAVGIAEYMLSAFLPSVSYADVFKYVNLAEYMKVYPLFSK
                     YHNLDFFDNPVNAMTVFRIVLPVVLVLFVLGNVRRFFRCAKTKRRWRRERKNSSRIGF
                     ISDKLYFYESVKCLFSNRAIWVCIAVMYGAVLVGNSIPTYRDIKEEYYKFYMTDQQGK
                     MTEEKVEYFNEERKRFEEIYSMTPENSDLTAVEIVQKQEENKYAHEGFSEAYSQVMYI
                     MSNNQGKGVNEQELVYEKGYQLLFGDKAVKERLIGILLCVIAAVYSASGVLGTEYDLK
                     VMNLLRSTKRGRKELFLKKLGVSFGITAVIFVLVKIPAILKVVGEYPLECWGAKVRSM
                     MFAGQSVINCSIFGYVLMLMIMQLVTLFVIVFSTMALSVVLKDSTMTMILSLLLFGGP
                     LLIEWGGVPIVHYLSLNSLLDGHQILQGNWLVLISEIVIFWGALSFAAGYVLYRAYEN
                     RR"
BASE COUNT         1558 a         1413 c          911 g         1595 t
ORIGIN      
        1 gaatgaattt acggaaatta aaatgtccgt aaaatgctcc gccgattcct cctgcaatac
       61 agcttatgat aaacggtttt tttagcggaa gaagcatacc atagatcgcc ggttctgtta
      121 caccgaaaat accggaaata aaattcggaa tcgccatctc ttttatcttt ttatcttttg
      181 ttttgaaaaa catcgcaagc actactgccg atgtagcgaa tgtacaggcg aaaaacggca
      241 tcataacatt gtcgtatccc aacgtctgca cattattaat ataaaccgga ataaatcccc
      301 agtgcattcc gaagattacc aaaatctgcc atgtaaatcc tacgaccgcg cctgcaagaa
      361 gcggactaaa ttccctgatc ataagcgtaa attcagagat cagcgtagat ccaaaggttg
      421 cgaccggtcc gaggaagatc attgcaaccg gaagagttac aagtaaagtc agcatcggca
      481 caaagaaaaa ctttacaaga tccggcacaa accgattaaa taatttttca cacttcgacg
      541 caaaccatac agtcagtatg accgggatca ccgttcccgt ataatctacc gaaatgaccg
      601 gaattccgaa aaagtctata tataccggag attggaacag cgttccttcc aaaacggtcg
      661 ttacagcctc tgctcctgcc gacaatgcag ataactggat acccggatag cacattgccg
      721 ctccgattgc aagaccgagc atcggtttta actgaaattt cttagctgct gtatatccaa
      781 ggaacagcgg caaaaaggta aacaacgcat cgcctgccgc attgataatc atatatcctc
      841 cgcaatcggc agaatacaat ccaagtgtaa cgaataatgt gttcagacct ttcagcattc
      901 cgcaggcagc catgatccct aaaataggct gaaaaatacc ggacactaca tcgatggcac
      961 gatccaggag cttccctgat ttctttgttt ccgcagacgc tttattctcg ttcaaatcga
     1021 gcagcgccat cacatcttca aatacttccg gcacatgatt tccgatcaca acctgatact
     1081 gaccgccgct tttcattaca gtcacaaccc cgtccatctt tttcagaaca tcgtcttttg
     1141 cgatactttc atcctttaac gtaaatcgaa gtcttgtgat acagtgaaca agtcctgtta
     1201 cgttttcttt tccgccgaca ttttttacga tatcttttgc cagcgtctca tactttccca
     1261 tttcttttcc tcctataact gttttttgat agttagaaaa taacatatgt ctttctttca
     1321 tacaattcgc acaagccgga ttgtttcagc gtttcaaaaa tcatgaaaaa gaaccgctgc
     1381 tttttgaaac agcggtccaa atttactcat ttttctcttt tgtatgatac gtttcatccc
     1441 aaagcagact taaatgattc aacatttcca tagaggatct tgcatgttct tcatatcgct
     1501 ttgacaacag aagcgcgaaa aaaatatcaa gaatataagt cgctcccgaa aaagaagtaa
     1561 tcacatccag gcttagcagc gcgtccctgt tcggaatctc cacaatctca tgacaccatt
     1621 tcttaagttc tgtatgataa ggacctgcaa ttccgaccac ataagtttct gtcgcctccc
     1681 ttaaatattt tgccatacag atcaccgtat gatttgatcc tgtcagactg atcaaaaaag
     1741 caatgttttt cttgtgctta cgcgccgcca gatagtgaga attaatactt tcgtatgccg
     1801 agcattccaa cccgagcgta ctgattttaa acgccgccga ctgtgcaagt atataactga
     1861 tccctgttcc gtaaatatcg atgcactctg ctttttgcaa tacttgactg atccgtttca
     1921 tactgttttt atctaaagaa agtcttgtat tcgtcaccgc tttatcatag agcgccggaa
     1981 gtgtttttac gatatcgtca tatgtacttt cggcagtcac cggctcgttt gcaagcagtt
     2041 gatcgatccg ctttttttga ttgatctcgg cagtaagagt aattttcaaa tcttgaaaac
     2101 catttaaccc aagcttttga ctcagcctca cgactgttgc cttactcgtg taagacgctt
     2161 tcgccagttc tcctaccgac agttccgaaa tctgatctaa atgacttaaa atatactccg
     2221 ctacatcttt ttcatgagcc gtaaaatttt tatgttcctg caatttttct aacagcatta
     2281 ttgtgcatag ctcccttcac ctcaaattca tacaagtaaa cgtcctccaa tgtcggagcg
     2341 gcttccctga tctcatcata cccttccgga cgcttctgtg tcactacgcg aacgataatc
     2401 tgtccgtctt ttcggaacag attacttatt tcaaactttt ctttcacttc ttccagctcc
     2461 tgctgcccta cactcatttc aaacactttt ccctgaatct ttgcctgtaa ttgttcggga
     2521 cttgccttat cgatcagccg tccgtttttc atcagaagaa tttctttgga aatgaattcg
     2581 atatctgata caacatgagt tgcgatcaaa acgatccgat cctgactcaa atccgaaata
     2641 agattacgga tgcggatacg ttctttcggg tctaatcccg ccgtcggctc atcgaggatc
     2701 aatatcttcg gatcgtttaa aagcgcctgc aaaagaagca ctctctgttt cattcctcct
     2761 gacagttcct tgatcttttt acgtttcacc ttgtcaagat ttaacaaagg aaataaaagc
     2821 tccatccgag cgcggatttc ctttttcttc attcctttca gcgttcccat ataagcgaga
     2881 aattcagtca ctgtaaacgt ttcgtacaac tgttgctgct gtggcatata tccgagcgat
     2941 tttctgtaag ccgcgcccca tttcaaaata tcttttccgc cgcacaatat tctccccgaa
     3001 tcccgaagga ggttatctgt caatatattc atcagcgtgc tcttccctgc cccgtttggt
     3061 ccgagtatgc cataaattcc gttttcaaac gtcgcgtcaa aattttgcag ggcttttttc
     3121 ttcccatagc ttttacataa ttcccgaatc ttaatttcca tccctgctac ctcctgttct
     3181 cataagcacg atataaaaca tatcctgccg caaatgaaag cgctccccaa aagatcacga
     3241 tttccgaaat aagtacaagc cagtttcctt gtaaaatctg atgcccgtcc aaaagactgt
     3301 tcagactaag ataatgtacg atcggaactc cgccccattc aatgagcagc ggaccgccga
     3361 acaaaagaag cgaaagtatc atcgtcatcg tagagtcttt caaaacgacg gacaatgcca
     3421 tcgtactaaa tacaatgaca aacaatgtca caagctgcat aatcatcagc atcaaaacgt
     3481 atccgaagat cgaacaattt ataaccgact gtcccgcaaa catcatcgaa cgcacctttg
     3541 cgccccaaca ttccagcgga tactccccga caacttttaa tatcgccgga attttgacta
     3601 aaacaaagat caccgctgta attccgaatg acacgccaag cttttttaaa aataattctt
     3661 tccgcccccg cttcgtactg cgaagcaggt tcattacttt cagatcatat tccgttccga
     3721 gaacgccgga tgcgctgtac accgcagcga tcacacataa aagaatcccg atcagacgtt
     3781 ccttcaccgc cttatctccg aacaaaagct gatagccttt ttcataaacc aactcctgtt
     3841 cattgactcc ctttccctga ttatttgaca taatgtacat cacctgactg tacgcctcgg
     3901 aaaatccttc atgagcatac ttgttttctt cctgcttctg aacaatctca acggcagtca
     3961 gatctgaatt ttccggagtc atagaataga tctcttcaaa tcgtttgcgc tcctcattga
     4021 agtattccac tttttcttct gtcatctttc cctgctgatc ggtcatataa aacttatagt
     4081 attcttcctt gatatccctg tatgtcggaa tagaattccc gacaagaacg gcgccgtaca
     4141 tgaccgcgat acatacccat atcgcacggt ttgagaaaag acatttcaca ctttcgtaaa
     4201 aatataattt atccgaaata aaaccgatcc gactgctgtt ttttctctcc cttctccatc
     4261 ttctctttgt tttggcacat cggaaaaacc tccgcacatt tcccaaaaca aaaagaacaa
     4321 gtactaccgg aagaacaatc cgaaatacag tcatcgcatt gaccggattg tcaaaaaagt
     4381 ctaaattatg gtatttagaa aacagcggat acactttcat atattccgcc agattaacgt
     4441 atttgaacac gtccgcataa gacaccgacg gaagaaatgc agacagcata tactctgcaa
     4501 ttccgaccgc tccgattccg atcattgcgc cggacggtgt ctctgatttt tgagcaatcc
     4561 atatcattcc taatatgatc acatagcaga ctgccatctt gacgatcaga tataaaaata
     4621 aaaacattcc tacggacatc atatacggac tttcgtacat cgtagttacc gactggatcg
     4681 gacgagagag gtcaccaaag ccatatttct gccacgcata aagataatcg actccgtaaa
     4741 acagaagaac gagcagcgcc gtcacacatg ccgccactgt cagctttgcc ccgatcacag
     4801 agcctctccc tcgataacag gatctcagca aaggatatag tttatttttc ttttcatcca
     4861 acacaagaaa atacactgtc accgcaagca taaaaacgat gcaaagatct gtaaattctg
     4921 cttctgtggc aaagatcact ccgtccgata catcactctt tagtttcagt cctttcagtt
     4981 tctcatacgc atccggggta atctgtgcat ttcgataagc aaacgttcct tcattcgaga
     5041 aaatagcgct ggacatactc gcgctttttt ccttgatctc ctcaagatat ttgtcgtatc
     5101 ggttgacctg tctgtactcc tgaaagattt tgttgtacaa catctcttct gcatattcat
     5161 tccccgtata aacaggaact ttcttttccg gaagcttctc gatccactct ttacgctttt
     5221 cttccggctg tgccttcaaa tctgcgtaaa tctgtttcac cgctcctgca tcgtacatct
     5281 ccatcttcat tttttcagac agcgtccaaa tattaaaaag aaaaaacacc gagcacaaaa
     5341 accaaaaata gcggaatgca cgaaactttt tccattcata cattacagat ttcatccgat
     5401 ccctcccctc cggcaaggtt tttttgtttc tcttactctt cagttacaat attccccgcc
     5461 gaagaaactc tcggcgt
//
LOCUS       DBMDXF010000124         5441 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3729_length_5441_cov_1.541011, whole genome shotgun sequence.
ACCESSION   DBMDXF010000124 DBMDXF010000000
VERSION     DBMDXF010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3729_length_5441_cov_1.541011"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="ACZPD4_05940"
     CDS             complement(<1..541)
                     /locus_tag="ACZPD4_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR1378637.1"
                     /translation="MDQENRLDVEQMIELLEKHDFKKLKEELESVHPVDIVDALEELD
                     KKQRILIFRLLAKEEAAEVFTDMNSDMREDLINALTDSELEEVMEEMYVDDTVDVLEE
                     MPANVVDRLLMATDEETRKKINALLQYPEDSAGSIMNIEYISLRKEMTVADAILKIRQ
                     VGINKETIYTCYVTEKRRLI"
     gene            707..2002
                     /locus_tag="ACZPD4_05945"
     CDS             707..2002
                     /locus_tag="ACZPD4_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIR1378638.1"
                     /translation="MKIVGLITEYNPFHNGHLYHIQQAKRTTGADAVIVVMSGDYVQR
                     GVPAVMPKRLRAESALECGASAVFELPVCYSTGSAELFAESAVALLDQLQIVDSLCFG
                     SECGDLDALSEIADLLCQEPKDYRSLLKKHLRNGLSFPCARRKALKEYLPEKNFAALL
                     DEPNNILGIEYLKAIRKLKSTIKPYTILRKGARYHDQELSTENLSSASAIRSLLSYSG
                     SSLRKYDDLPKGAFDDTAPFNIFGELESQVPQCCLKLLKDCHKVSYPIYQNDFSLILK
                     YKLLNKYPENLLRYMDVSEELANRICSRLNYFFNYKQFCDLLKTRELTQTRINRALLH
                     IMLGIKKTDVEEYIDGGYHFYARLLGFRKDREKLLTRISKESSLPLLIRLSETETIPP
                     LGRKMLRNDMLASNLYTSVVTDKFKTAFQNEYKQKVIKI"
     gene            complement(2084..3280)
                     /locus_tag="ACZPD4_05950"
     CDS             complement(2084..3280)
                     /locus_tag="ACZPD4_05950"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR1378639.1"
                     /translation="MGKIKMTTPLVEMDGDEMTRILWKMIKDELLIPYIDLKTEYYDL
                     GLEHRNETDDQVTVDSAMATKKYKVAVKCATITPNAARMDEYDLKEMWKSPNGTIRAI
                     LDGTVFRAPIVVKGIEPCVKNWKKPITIARHAYGDVYKGSEMKIPGAGKVELVYTAED
                     GSETKELVHNFDGPGIVQGMHNINRSIESFARSCFSYALDTKQDLWFATKDTISKKYD
                     HTFKDIFQEIYDAEFDEKFKEAGIEYFYTLIDDAVARVMKSEGGYIWACKNYDGDVMS
                     DMVSSAFGSLAMMTSVLVSPEGYYEYEAAHGTVQRHYYKHLKGEETSTNSVATIFAWT
                     GALRKRGELDGNTELMEFADKLEKATIDTIEAGEMTKDLALITTLENPTVLNSEQFIK
                     AIAKRL"
     gene            complement(3379..4035)
                     /locus_tag="ACZPD4_05955"
     CDS             complement(3379..4035)
                     /locus_tag="ACZPD4_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1378640.1"
                     /translation="MEEYQDHSLGSRVFQKIRDNILNGTYKENDELRETAIGKELGVS
                     RTPVREALRQLELEGLVTIIPNRGAYVSGISHKDIWDIYKIRSMLEGLCARWAAEHIT
                     EEQLDQLEESLLLSEFQMKKESGFSTEQVAALDGRFHAVLYEASGSRMLKHVLTDFHN
                     YVQRARKSSIVSEERARKSIREHRQILRAIKDRDAEMAEQLANEHIIHVMQNLKKQGY
                     "
     gene            complement(4057..>5441)
                     /locus_tag="ACZPD4_05960"
     CDS             complement(4057..>5441)
                     /locus_tag="ACZPD4_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR1378641.1"
                     /translation="NRKVFLNKHTNLLELEEHMYPLVDVDTPNVFRNLFHYDEIPKIA
                     FNDRIVPHNMPDEIWITDTTFRDGQQSRAPYTTEQIVSIYEYLHRLGGPKGKVRQSEF
                     FLYSKKDRDAVYRCLEKGYEFPEITSWIRASKKDFELVKEIGLKETGILVSCSDYHIF
                     YKLKMTRKEALEHYLTVVRECMETGVRPRCHLEDITRSDIYGFVIPFCMELMKLMDEY
                     KIPVKIRVCDTMGYGVNFPGAVIPRSIPGIIYGLMTHAGVPSELIEFHGHNDFYKAVN
                     NSTTAWLYGACGVNCSLFGIGERTGNTPLEAMVFEYAQLRGTLDGMDTTVITELAEYY
                     EKEIGYTVPSRTPFVGKNFNVTRAGIHADGMLKNEEIYNIFDTDKFLNRPALVSVSNT
                     SGTAGIAYWINAYFKLLEERKVDKNSELVHRLKEWVDKQYEDGRVTVLTDEELIAKIE
                     SVCRELHVVL"
BASE COUNT         1489 a         1366 c          877 g         1709 t
ORIGIN      
        1 cgatcaatct tcttttttcc gtcacatagc atgtataaat cgtctcttta ttaataccga
       61 cctgacgaat ctttaaaata gcatccgcaa ccgtcatctc tttacgaagg ctgatatact
      121 cgatattcat aatgctgccc gcactgtcct ccggatactg caaaagcgca tttatcttct
      181 ttcttgtctc ttcatccgtt gccatcaaaa gacgatccac tacatttgcc ggcatttctt
      241 ctaatacgtc gacggtgtca tcaacgtaca tctcttccat aacttcttcc aactccgaat
      301 ccgtcagcgc attgatcaaa tcttctctca tgtcgctgtt catatccgta aacacttccg
      361 ccgcttcttc ttttgccaga agacggaaga tcaaaatacg ttgttttttg tccaattctt
      421 ccaatgcatc tacaatgtct accggatgca cagactccag ttcttccttt aattttttaa
      481 aatcatgctt ttcaagcagt tcgatcatct gctctacgtc aagtctgttt tcctgatcca
      541 tgcctaactc ctcatcttcc aagatatctt tttgtaacaa ttcagccata ataggcacgc
      601 agtgcggttt tatgaacaac acggactgaa ccgttacata ttccactgta aaaattcatc
      661 tttttatagt ataatatggc agaaaatgat atacaaggag tttcttatga aaattgtagg
      721 acttattaca gaatataatc catttcacaa tggacatctc taccatatcc aacaggcaaa
      781 acgtacaacc ggtgccgacg ctgtcatcgt tgttatgagc ggcgattatg tccaaagagg
      841 tgttccggct gtcatgccaa agcgtttaag agccgaatcc gctctggaat gcggtgcttc
      901 cgctgtattt gaacttcctg tctgctattc taccggaagc gcagaattgt ttgccgaaag
      961 cgctgtcgct ttgttggacc agcttcaaat tgtagactcg ctttgtttcg ggagtgaatg
     1021 cggagattta gatgctttat ccgaaattgc agaccttctc tgtcaagagc caaaagatta
     1081 ccgttctctt ttaaaaaaac atttaagaaa cggactttcc tttccgtgtg cacgcagaaa
     1141 agctttgaaa gaatatcttc cggaaaaaaa ctttgcggct cttttagatg aacctaataa
     1201 tattctcgga atcgaatacc taaaagcgat cagaaaatta aaaagcacga tcaaaccata
     1261 tacgatcctc agaaaaggcg cccggtatca cgaccaagaa ctcagtacag agaatttaag
     1321 ttctgcatcg gcgatccgtt ctcttctttc gtattcagga tcttctttaa gaaaatatga
     1381 cgatctacca aagggagctt ttgacgacac cgcccctttt aacattttcg gagaattgga
     1441 atcacaagtg ccgcagtgct gcctgaaact tttaaaagat tgccacaaag tttcttaccc
     1501 gatttatcaa aacgactttt ctcttatcct gaaatacaaa ttattaaaca aatatcccga
     1561 aaacctgctt cgatacatgg atgtctctga agaactggca aacagaatct gttcccggct
     1621 taattacttt ttcaactaca aacagttctg tgaccttcta aaaacaagag aacttacgca
     1681 gacacggatc aaccgggcac tccttcatat aatgctcgga ataaagaaaa cagacgtaga
     1741 ggaatatatt gatggcggat atcattttta cgcccgcctt ttaggctttc ggaaagaccg
     1801 ggaaaaactg ctcacacgca tttcaaaaga aagttcactg ccgcttctta tccggttgtc
     1861 tgaaaccgaa accatccctc ctctaggccg caaaatgctt cgcaatgaca tgcttgcatc
     1921 taatttatac acttccgtcg tcaccgataa gtttaaaaca gccttccaaa atgaatacaa
     1981 gcagaaggtg atcaaaattt aatttccgcc agacaaaaac atgcccgcag cggtcttttt
     2041 ccaccactgc ggacatgttt ttatttttct gttatttcaa tcattacagt ctctttgcga
     2101 ttgctttaat aaactgctcg ctgttgagta ccgtcggatt ttcaagcgtt gtgatcaatg
     2161 cgagatcttt tgtcatctct cccgcctcga tcgtatctat cgttgctttt tccaacttat
     2221 ccgcaaactc catcaattcg gtattgccgt caagttctcc tctttttcta agcgcacctg
     2281 tccatgcaaa aatcgttgcc actgagtttg tcgatgtctc ttcccctttt aaatgcttat
     2341 agtagtggcg ctgaacagtt ccgtgagccg cttcatattc atagtatcct tccggtgaaa
     2401 cgagaacaga tgtcatcatc gccaacgaac caaaggcaga agacaccata tcactcatca
     2461 catctccgtc gtagtttttg catgcccaaa tatatccgcc ttctgatttc attacacggg
     2521 caaccgcatc atcgataagc gtgtaaaaat actcaatgcc tgcttctttg aacttctcat
     2581 caaactcagc gtcataaatc tcttgaaaaa tatctttaaa tgtatgatca tactttttgg
     2641 aaatcgtatc ttttgtggca aaccaaagat cctgctttgt atcaagagca taagaaaaac
     2701 agcttcttgc aaaactttcg atcgaacggt tgatattatg cattccctgc acaatacccg
     2761 gtccgtcaaa attatgtaca agctctttcg tctcgcttcc gtcctctgct gtatatacaa
     2821 gttcaacttt gccggctccc ggaattttca tctcagaacc tttgtataca tcaccatatg
     2881 catgtcttgc aatcgtaatc ggtttcttcc aattcttcac acacggctcg attcctttca
     2941 ccacgatcgg agcacgaaat accgttccgt caagaatcgc acgaatcgta ccgtttggac
     3001 ttttccacat ctctttcaaa tcatactcat ccatgcgcgc tgcatttgga gtaattgtag
     3061 cacattttac cgcaacctta tatttcttgg tcgccattgc agaatcaacc gtgacctgat
     3121 cgtctgtctc gtttctatgc tctaatccaa gatcataata ttctgttttt agatcaatat
     3181 acggaataag aagctcgtct ttgatcattt tccaaagaat tcttgtcatc tcatctccgt
     3241 ccatctccac aagcggtgtc gtcattttga tttttcccat tgttattttc tcctttttaa
     3301 tatatgaact ttttgccccc atctattcaa cacatcttcc gcttatacag cttccgatgt
     3361 agctgtactt tttgcaatct aatatccctg ctttttcaaa ttttgcatta catgaataat
     3421 atgctcatta gcaagctgtt ctgccatctc tgcatctctg tctttgatcg cacgtaaaat
     3481 ctgtctgtgt tcacgtatcg actttctggc tctttcttct gatacgatcg atgattttct
     3541 ggctctttgc acataattat gaaaatccgt cagaacatgt ttcagcattc tgcttcccga
     3601 agcttcatag agaacagcgt gaaaacgacc atccaacgcc gcaacctgct ccgtgctgaa
     3661 gccgctttcc tttttcattt gaaactctga aagaagaagc gattcttcca actggtcaag
     3721 ctgctcttct gtaatatgtt ctgccgccca gcgggcgcac aatccttcca gcatcgagcg
     3781 gatcttatag atatcccata tatctttatg agatataccc gatacataag ctcctctgtt
     3841 cggaataatc gtcacaagcc cttccaactc aagctgacga agagcctccc gtacaggcgt
     3901 ccggctcact cctaattctt tccctatcgc cgtttcccta agttcgtcgt tttctttata
     3961 tgttccattt aaaatattat cccttatctt ctgaaacaca cgacttccaa gggaatgatc
     4021 ctgatattct tccatctgaa atcctccgac tataatctac aatacaacat gcagttccct
     4081 acatacactt tctattttgg caattaattc ttcatctgtc aagaccgtaa cgcgtccgtc
     4141 ctcatactgt ttatcaaccc attctttcag acgatgtacc agttccgaat ttttatcaac
     4201 tttccgttct tccagaagtt taaaatacgc attgatccaa tatgcgatcc ccgctgttcc
     4261 ggatgtattg gaaaccgaca ccagtgcggg acggtttaaa aatttatccg tatcaaaaat
     4321 attgtatatt tcctcatttt tcaacatgcc gtccgcgtga atccccgcac gcgtcacatt
     4381 aaaattctta cctacgaaag gcgttcttga aggtacagta tatccgattt ctttctcata
     4441 atattctgca agctccgtga tcacagttgt atccatacca tccaaagttc cgcgaagctg
     4501 cgcatactca aataccattg cctcaagggg agtatttccg gttctttccc cgataccaaa
     4561 caatgaacag tttacaccgc acgccccgta aagccacgct gtcgttgaat tattcaccgc
     4621 tttataaaaa tcattatgtc cgtggaattc gatcagttca cttggaacac cggcatgagt
     4681 cattaagcca tatatgattc ccggaatcga tcttggaatt acagctcccg gaaaattcac
     4741 tccgtatccc atcgtatcac aaacacggat ttttaccggt attttatatt catccatcaa
     4801 tttcattaat tccatacaaa acggaatcac aaatccgtaa atatccgatc tcgtaatatc
     4861 ttccagatga catcgcggac gaactcccgt ctccatacac tcacgaacta cagtaagata
     4921 atgttcaagg gcctcttttc tcgtcatttt caacttataa aaaatatgat aatccgaaca
     4981 gcttacaaga atccccgtct cttttaatcc gatctccttc acaagctcaa aatctttttt
     5041 gcttgctctg atccaactcg taatctccgg aaattcatac cctttttcca ggcagcgata
     5101 gacagcatct ctgtcttttt tactgtataa aaagaattcg ctctgccgta cttttccttt
     5161 cgggccgccg agtctgtgca aatattcata aatcgaaaca atctgttcag tcgtatatgg
     5221 agctctcgac tgctgaccat ctctgaatgt tgtgtctgtg atccatattt catccggcat
     5281 attatgaggt acaatcctgt cattaaatgc aatcttcgga atctcatcat aatgaaacaa
     5341 attacgaaat acattcggtg tatccacatc aacaagggga tacatatgtt cttctaattc
     5401 aagcaagttc gtatgcttat tcaaaaatac ttttctgttt t
//
LOCUS       DBMDXF010000125         5374 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3782_length_5374_cov_1.965105, whole genome shotgun sequence.
ACCESSION   DBMDXF010000125 DBMDXF010000000
VERSION     DBMDXF010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3782_length_5374_cov_1.965105"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="ACZPD4_05965"
     CDS             <1..1879
                     /locus_tag="ACZPD4_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1378642.1"
                     /translation="SLKKEENEPEKRIDHKVSGVSFATTLMSEAEIPKKGRRKNKSPD
                     VCELIPDNEAFVINREEPFMEAEPIRQEGKEQQYEALQQTDTFTPLEEKAEEDLQNQK
                     NTSGRKNKKEDSQAVARETQNVADAVRIEEQKEHPVYHTPPISLLKKGRKAGGDSDAH
                     LRATALKLEQTLRNFGVGVHVTNASCGPSVTRYELQPEQGVKVSKIVGLSDDIKLNLA
                     VADLRIEAPIPGKAAVGIEVPNSENTAVMLRDLLESKEFQASASPISFAVGKDIAGKV
                     VVSDIAKMPHLLVAGATGSGKSVCINTLIMSIIYKADPEEVKLILVDPKVVELSVYNG
                     IPHLMIPVVTDPKKAAGALNWAVAEMEKRYKLFADYNVRDLKGFNEKIEQGQTGEDIQ
                     KKLPQIVIIIDELADLMMVAPGEVEGAICRLAQLARAAGLHLILATQRPSVNVITGLI
                     KANMPSRIAFSVSSGVDSRTIIDMNGAEKLLGKGDMLFYPSGYPKPVRVQGSFVSDKE
                     VQNVVDYLINHSERVSYNNELEEHMVTNIVSSGNGMQAGENGDDRDTYFIEAGKLIIE
                     KEKASIGMLQRMFKIGFNRAARIMDQLAQAGVVGPEEGTKPRKVLMTKEEFENYLQKG
                     N"
     gene            1890..3560
                     /locus_tag="ACZPD4_05970"
     CDS             1890..3560
                     /locus_tag="ACZPD4_05970"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1378643.1"
                     /translation="MKTDIQIAQEAKMEHIKDVAARAGIEENELEFYGKYKAKLSDEL
                     WEKIKDREDGKLVLVTAINPTPAGEGKTTTSVGLGQAMAKLNKNAIIALREPSLGPCF
                     GIKGGAAGGGYSQVVPMEDLNLHFTGDFHAITSANNLLAAMLDNHIQQGNELQIDPRQ
                     VVWKRCLDMNDRVLRNIVVGLGNKMDGMVREDHFVITVASEIMAILCLADDLADLRAR
                     LGRIIVAYNFAGEPVTADDLHATGAMTALLKDAVKPNLIQTLEHTPALVHGGPFANIA
                     HGCNSVRATKMALKLSDITITEAGFGADLGAEKFFDIKCRMADVKPDAVVLVATVRAL
                     KYNGGVAKADLAEENLDALAKGIVNLEKHIENIQKYKVPVIVTLNSFVTDTDAENEFI
                     CRFCEERGCEFALSEVWEKGGEGGIALAEKVLDTLENKKSDFELLYEDSLSLEEKIEK
                     IAKEIYGADGVVYEPAAKKQIAKIESLGFGSFPVCMAKNQYSLSDDAKKLGRPHGFDI
                     HIREVYVSAGAGFVVALTGAVMTMPGLPKVPAANGIDVTEDGTITGLF"
     gene            3572..4426
                     /gene="folD"
                     /locus_tag="ACZPD4_05975"
     CDS             3572..4426
                     /gene="folD"
                     /locus_tag="ACZPD4_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR1378644.1"
                     /translation="MPYIIDGKAISKQIKDELRDEVEKLTEAGINACLAVIQVGNDPA
                     STVYVNNKKKACAYVGINSRSYELPEDTSEQTLLDLVQKLNEDPEVNGILVQLPLPKQ
                     IDEDKVIRTISPDKDVDGFHPVSVGRLWIGEKGFLSCTPAGVIQLLKRSGIEIEGKEC
                     VIIGRSNIVGKPMAALLLRENATVTVAHSKTKDLKSIAKRADILIVAIGKAKFITKDY
                     IKEGAVVIDVGMHRDEQNHLCGDVDCEDVSSVVSAITPVPGGVGPMTIAMLMNNCVET
                     IRGREERA"
     gene            4423..5319
                     /locus_tag="ACZPD4_05980"
     CDS             4423..5319
                     /locus_tag="ACZPD4_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1378645.1"
                     /translation="MKCVHCGANIPDDQMICPVCGAEVQIVPDYNPLDDVLAREVRGS
                     VEGATRQIRTDDIRRYRREDLTKNINSTRVLSEDEMERIRSNRRKSTNNTERNSAELR
                     RQRQARQQKRLEAAKRKRRRLLLTLAAVVILIGFGIYVAYQNSYTGMLKKGYRAVNEK
                     EYMAAEKYFDRAIIKDKSRPDAYVGLAEIYLDQNDTDGAEDVYLSAIETQPTNEKLYQ
                     AAIDFYMETKQPEKVASLLEDCEDDNVLASVSEYVCEAPEFKPEEGTYKEVQEITISS
                     DTEGEIYYDGRNRSDCEDREKI"
     gene            5273..>5374
                     /locus_tag="ACZPD4_05985"
     CDS             5273..>5374
                     /locus_tag="ACZPD4_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024683.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HIR1378646.1"
                     /translation="MTDGTDPTAKTGKKYKEPILLEKEGTTEIRAIVV"
BASE COUNT         1752 a          904 c         1417 g         1301 t
ORIGIN      
        1 atctttgaaa aaagaagaaa acgagccgga gaaaaggatc gatcataaag tgtccggtgt
       61 ttcatttgca acgactctta tgtctgaagc agaaataccg aaaaaaggca gaaggaagaa
      121 taaatcgccg gatgtgtgcg aattgatacc tgataacgaa gcatttgtca ttaatcgtga
      181 agaacctttt atggaagcag aacctattcg gcaagagggg aaagaacagc agtatgaggc
      241 tttgcagcag acagatacat tcacgccttt agaagaaaag gcagaagagg atcttcagaa
      301 tcagaaaaat acgtcaggaa gaaaaaacaa aaaagaagat tcccaagcag tggcaaggga
      361 aactcagaac gtggccgatg cggtgagaat agaagagcag aaggaacatc ctgtttatca
      421 tacacctccg atcagtcttc tgaaaaaagg aaggaaagcc ggtggagatt cagatgcgca
      481 tcttagagct acggcgctta agttagagca gacattacgc aatttcggag ttggcgtaca
      541 tgtgacgaat gcaagttgtg gaccgtccgt tacaagatat gaactgcagc cggagcaagg
      601 ggtgaaagta agtaagattg tcggtctttc agatgacatt aaattaaatc ttgcagtggc
      661 ggatcttcgt atcgaagctc cgattccggg aaaagcggca gtgggaattg aagtgccgaa
      721 cagtgagaat acagcggtta tgcttcgtga tcttttggaa tcaaaagaat ttcaggcaag
      781 cgcttctccc atttcgtttg cggtcggaaa agatattgcg ggaaaagtag tcgtttcgga
      841 tattgcgaag atgccgcacc ttcttgtggc aggagcaacc ggatcgggaa aatcagtttg
      901 tatcaatacg ttgatcatga gtattatcta caaagcggat ccggaagaag tcaaactgat
      961 cttagtcgat ccgaaagttg tggagttgag cgtatataac gggattccgc atctgatgat
     1021 ccctgttgta acagatccga aaaaagcggc aggtgctttg aactgggctg tagcagagat
     1081 ggagaagcgt tataaactgt ttgctgatta taacgtgcgt gatctgaagg gatttaatga
     1141 aaagatagaa caaggtcaga caggagagga tattcagaaa aagcttcctc agatcgtaat
     1201 tattattgat gagctggcag accttatgat ggttgctccg ggagaggtag aaggtgctat
     1261 ttgtcgtctt gcacagcttg cgcgggcggc aggactgcat ttgatccttg cgacacaaag
     1321 accatctgtc aatgtcatta ccggtcttat taaagcaaat atgccctctc gtattgcatt
     1381 ttcggtttcg tcaggtgttg actcaagaac aattatagat atgaatggcg cggaaaaact
     1441 gctcggtaaa ggggatatgc ttttttatcc gtcaggatat ccgaaacccg ttcgtgtgca
     1501 aggttcattc gtatcagata aagaagttca aaatgtagtc gattatctga tcaatcacag
     1561 tgaaagagtt tcttataata atgaactgga agagcatatg gtcactaata ttgtgtcatc
     1621 gggaaatgga atgcaggcag gtgaaaacgg tgatgaccgt gatacatatt ttatagaggc
     1681 cggaaagctt attatagaaa aagaaaaagc atctatcgga atgcttcaga gaatgtttaa
     1741 aatcgggttt aaccgggcgg caaggattat ggatcagctt gcccaagccg gagttgtagg
     1801 tcctgaagaa ggaacaaaac cgagaaaagt attaatgaca aaggaagagt ttgaaaacta
     1861 tttacaaaag ggaaattagg aggtagaaaa tgaagacaga tattcagatc gcacaggaag
     1921 caaaaatgga acacatcaag gatgtggcgg cacgggcagg aattgaggag aacgagctgg
     1981 agttttacgg aaagtataaa gcaaaacttt cagatgagtt atgggagaag attaaagatc
     2041 gtgaagacgg aaagcttgtt cttgtgacag ccattaatcc aactccggca ggcgaaggca
     2101 agacgacaac atctgtcggt ttaggtcagg caatggcgaa attgaataaa aatgcgatta
     2161 ttgcacttcg agaaccatct ttaggacctt gtttcggaat caaaggcgga gcggcaggcg
     2221 gcggctattc gcaggttgtg ccgatggaag atctgaatct tcattttacg ggagattttc
     2281 atgcaatcac gtcggcaaat aacttgcttg cggcgatgct tgacaatcac attcagcagg
     2341 gaaatgaact tcagatcgac ccgagacagg ttgtatggaa acgctgtctg gatatgaatg
     2401 atcgtgtgct gagaaacatt gttgtaggac ttggaaataa gatggatgga atggtgcggg
     2461 aagatcattt tgttattaca gttgcgtctg aaattatggc gattctttgc cttgcagatg
     2521 atcttgcgga tttaagagct cgcctgggca gaatcattgt agcgtataat tttgcgggag
     2581 agccggtgac agcagatgac ctgcatgcaa caggggcgat gaccgctttg ttgaaagatg
     2641 cggtgaaacc aaaccttatt cagacacttg aacatacacc tgcgcttgta catggcggac
     2701 cgtttgcaaa tatcgcgcat ggatgcaaca gtgtgcgcgc aacaaagatg gcgctgaaat
     2761 taagtgatat tacgattaca gaagcgggat tcggagcaga tctcggagca gagaaattct
     2821 tcgatatcaa atgtcgtatg gcagatgtca aaccggatgc agttgttttg gttgcaactg
     2881 tacgagcttt gaagtataat ggtggggttg cgaaagcaga tttagcggaa gaaaatctgg
     2941 atgcactcgc aaaaggaatt gtaaatcttg aaaaacatat cgaaaatata caaaaatata
     3001 aggttcctgt tattgtaacg ttaaattctt ttgttacaga tacagatgcg gaaaatgaat
     3061 ttatctgcag gttctgcgaa gaaagaggct gtgaatttgc tttgtccgaa gtatgggaaa
     3121 aaggtggaga aggcggtatt gcacttgccg aaaaagtatt ggatacattg gaaaacaaaa
     3181 aatcagactt tgaacttctt tacgaagatt ccctttcttt agaagaaaag atcgagaaaa
     3241 tcgccaaaga aatttacgga gcagacggtg tcgtatatga gccggcagca aaaaaacaga
     3301 ttgcaaagat cgaatcattg ggattcggaa gttttccggt atgtatggca aagaatcagt
     3361 actctttgtc tgatgatgca aaaaaactcg gaagaccgca cggctttgac atccacatcc
     3421 gcgaagtata tgtaagtgcc ggagcaggat ttgtagttgc gctgacaggt gcggttatga
     3481 cgatgccggg actgccgaaa gttccggctg caaacggaat cgatgttacg gaagacggaa
     3541 cgataacagg cttgttttaa aggagaaata aatgccatac attattgatg gaaaagcaat
     3601 ctcaaaacag attaaagatg agttgcgcga cgaagtagaa aaattaacag aggcaggcat
     3661 aaatgcctgc cttgcagtga tacaggtggg aaatgaccct gcttcaactg tatatgtgaa
     3721 taataaaaag aaagcatgcg cgtatgtcgg gatcaattcc cggtcatatg aactgccgga
     3781 ggatacatcg gaacaaacat tgttggatct tgtacagaaa ttgaatgaag atccggaagt
     3841 aaacggcatt cttgtgcagc ttccgctgcc gaaacagatc gatgaggaca aggttatccg
     3901 tacaatttct ccggataaag atgtggatgg ctttcatccg gtaagtgtgg gaagactctg
     3961 gatcggagag aaaggttttt tgtcatgtac tcctgcagga gtgatccaac ttttgaaacg
     4021 atcgggaatt gagattgaag gaaaagaatg tgtgatcatc ggcagaagca atattgtagg
     4081 aaagccgatg gcggctcttt tgctcagaga aaatgcaact gtaacagttg cccattcaaa
     4141 aacaaaagat ttgaagagta ttgccaaaag ggcggatatc ttgattgtag cgatcggtaa
     4201 agcaaaattt attacaaaag attatataaa agaaggagct gttgtcatcg atgtaggaat
     4261 gcatagagat gaacagaatc atctctgcgg agatgtggac tgtgaagatg tatcttcggt
     4321 cgtttcggca atcactccgg tgccgggcgg cgtcggaccg atgacgatcg ccatgttgat
     4381 gaataattgt gtggaaacaa tacgcggtcg ggaggaaaga gcatgaaatg cgtgcattgc
     4441 ggagcaaata ttcctgatga tcaaatgatc tgccccgtat gtggagccga agtacagatt
     4501 gttcccgatt ataacccgct ggatgatgta cttgcaagag aagtaagagg gtcggttgaa
     4561 ggagcaacac gacagatacg gacagatgat atcagaagat acagaagaga agatctgaca
     4621 aaaaatatta attctacccg tgttttaagc gaggacgaga tggagcgtat tcgctcaaac
     4681 cggagaaaaa gtacaaataa tactgaaaga aattcggcag aactgcgccg acaacgtcaa
     4741 gcccggcagc aaaaacgtct ggaagcggcg aaaagaaaga ggagaaggct tttgcttaca
     4801 ctggcggcag ttgttatatt gatcggattt ggtatttatg tggcatatca gaattcttat
     4861 acaggaatgc tgaaaaaagg gtaccgtgca gttaatgaga aagaatatat ggcggcagaa
     4921 aaatatttcg atcgtgcgat cataaaagat aagtcaagac cggatgcata tgtaggtctt
     4981 gcggaaattt atctggatca aaatgataca gacggtgcgg aagatgttta tttgagcgcg
     5041 attgaaacac agcccacaaa cgaaaaattg tatcaggcag caatcgattt ttatatggag
     5101 acaaaacagc ctgaaaaggt ggcatctctt cttgaagact gtgaagatga taacgtattg
     5161 gcttcggtgt ccgaatatgt ttgtgaagct cctgaattta aacccgaaga gggaacatac
     5221 aaagaggttc aggagattac gatcagttcg gatacggaag gtgaaatcta ttatgacgga
     5281 cggaacagat ccgactgcga agacagggaa aaaatataaa gaacctatct tactggaaaa
     5341 agaaggaaca acggagattc gcgctattgt tgta
//
LOCUS       DBMDXF010000126         5363 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3792_length_5363_cov_1.724439, whole genome shotgun sequence.
ACCESSION   DBMDXF010000126 DBMDXF010000000
VERSION     DBMDXF010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3792_length_5363_cov_1.724439"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /gene="recD2"
                     /locus_tag="ACZPD4_05990"
     CDS             <1..1922
                     /gene="recD2"
                     /locus_tag="ACZPD4_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1378647.1"
                     /translation="KEDTFRIIEEEPERLAEIKGISERKAREISSQLEDKKDLRKAMI
                     YLQKYGISAKLAAKIYNHYGMRVYKVMEENPYELADNIEGIGFRTADEIAARIGIHTD
                     SDYRIKSGLFYTLQQAVGEGHIYLPQEELLRRARTLLEVEIDDIEKHVMDLCIEKKVV
                     MKECVNENKNENEIRIYPAHYYYMELNTAKMLHDLNINCDMPEEMMERRLCKVERAEE
                     IKLDPKQHTAVIESIKHGLLVLTGGPGTGKTTTINTMIRFFESEGMSILLAAPTGRAT
                     KRMTEATGYEAQTIHRLLEVNVNPEEAENSGGFLRNRQNPLEADVIIIDEMSMVDLTL
                     MHALLSAVIPGTRLILVGDVDQLPSVGPGSVLKDIIASHRFPVVTLTRIFRQAGESDI
                     IVNAHKINAGEPVIPDNKSRDFFFLRRQDADTIIGIVIMLIQKKLPKYVGADQNEIQV
                     MTPTRKGLLGVERLNEILQKYLNPEEPKKAQREINGRLFREGDKVMQIKNNYQIEWEV
                     STKFGLTVDKGTGVFNGDMGVITEINEYTETIEVEFDESRKVKYGFDMTEELELAYAI
                     TVHKSQGSEYPAVIIPLLPGPKLLYNRNLLYTAVTRAKKCLTIVGSEAVFQEMIQNKN
                     EQARYTSLAERIQEF"
     gene            1916..2602
                     /locus_tag="ACZPD4_05995"
     CDS             1916..2602
                     /locus_tag="ACZPD4_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR1378648.1"
                     /translation="MLNLLYPKVCPFCGEISPEGVCAECKKKIIYVRQPRCMRCGKPI
                     KDEYKEYCSDCAKRTSHITQGRSLWLHKEPVSTAIYKFKYNNKRSWGELFAAEMAQAY
                     KDQVIKWGIEEIIPIPLHKSRKRKRGYNQAEIIAKCLSEKTGVSLVSDALFRVKKTTA
                     QKKLDRQERMGNLRDAFALSERWKPVANVLLIDDIYTTGATVEQAAKILKKAGAQNVY
                     FLTISIGQGI"
     gene            2660..3097
                     /locus_tag="ACZPD4_06000"
     CDS             2660..3097
                     /locus_tag="ACZPD4_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378649.1"
                     /translation="MDFGKYIGGREDVMLDKRKIRLMTRAAIYEKNYGEEDFKISSYY
                     KKDYSSLNTWVTLIWITVGYGLAVAVLFLGWGEAAMEGITLAKLFVFAAVAIGAYVSL
                     LIAYGIGAGSFYKNKHIKAKQRVKKYLRDLSRIEKINNKKEIN"
     gene            3100..4158
                     /locus_tag="ACZPD4_06005"
     CDS             3100..4158
                     /locus_tag="ACZPD4_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378650.1"
                     /translation="MSTLLVWREKLQRIYALYSIYIQKGLQFVTGLVLFWVINSNIGF
                     MKPASSVFCTLGLAVICTFLPMTITVLAATALILAHFYALSLPIALVALVIFILMYIF
                     YFRFTPKKAWLVLISGLAFSLKIPFVIPIAFGLLGSPVWIVPASCGIITYYMTDYVKV
                     SAAALKEADASGIAANLMSFTKQVLASKEMWLMVMAVTIGILVVNFVRTRGVDHSWKI
                     ASAAGAAVTVVVSAAGNIVLSTNISYIEIILSAVLGAVTGLVLELLFFSVDYSRTENI
                     QFEDDEYYYYVKAVPKVGVAVPEKKVKRITENQPQETTVIREDADLNNINTENDADDI
                     LLTRSLSKELGLEKQRPE"
     gene            4191..5054
                     /gene="cdaA"
                     /locus_tag="ACZPD4_06010"
     CDS             4191..5054
                     /gene="cdaA"
                     /locus_tag="ACZPD4_06010"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR1378651.1"
                     /translation="MLDWIKSILDNYTNGMYFPNIHIKDVIEILIVAVIVYEIMLWIK
                     NTKAWMLLRGIIMLGVFILLAWIFQMHTILYLAAQSINVLAIAAVVVFQPELRRALEK
                     LGEKNLLSNINPFDKNRENERFSDQTAEGIVTACFEMGKVCTGALIVVEQAIQLTEYE
                     MTGIELDCKVSPQVLINIFEHNTPLHDGAIIIRGDRITSATCYLPLSDNMRISKDLGT
                     RHRAALGMSEVCDALVIAVSEETGLVSVAMGGQLDRGITREELRKRLSQIQYRNAESK
                     RFFLRKGWRRK"
     gene            5051..>5363
                     /locus_tag="ACZPD4_06015"
     CDS             5051..>5363
                     /locus_tag="ACZPD4_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1378652.1"
                     /translation="MKKYKFTDNLGLKIIAVIFAAFLWLIVVNLDNPVSTQTFSEVPV
                     TIINEDIILSAGDTYQVLGEEKVSVVVSATRQVRQKLTKEDIVATADICGFEGVREQL
                     DL"
BASE COUNT         1699 a          815 c         1388 g         1461 t
ORIGIN      
        1 ttaaagaaga tacttttcgt atcatagaag aagaaccgga gcgtcttgcc gaaatcaaag
       61 gaatcagcga gcgtaaagca agagagattt catcacagtt agaagataaa aaggatttgc
      121 gaaaggcaat gatctatctt caaaagtatg gaatttctgc gaaattggca gcaaagatat
      181 ataatcatta tggtatgcgt gtatacaaag tgatggaaga aaatccgtat gaacttgcgg
      241 ataatattga ggggatcgga tttcgtaccg cagatgagat tgcggcacgc atcggaatcc
      301 atacagattc cgattaccgg attaaaagcg gactgtttta tacattgcag caggcagtag
      361 gagaagggca tatctatctt cctcaggagg aacttttacg tcgtgcaaga acgcttttgg
      421 aagtggagat tgacgatata gaaaagcatg tgatggatct gtgcattgag aaaaaagtgg
      481 ttatgaaaga atgtgtcaat gaaaacaaaa atgaaaatga aatcaggatt tacccggcac
      541 attattatta tatggaattg aatacggcga aaatgcttca tgatttgaat ataaattgtg
      601 atatgccgga ggaaatgatg gaaagacggc tctgtaaagt ggagcgggca gaagagatca
      661 aattggatcc taagcagcat acagcggtga ttgagtcgat caaacacggt ttgcttgtgt
      721 tgaccggagg accgggaacg ggaaaaacaa cgacaattaa tacaatgatc cgtttttttg
      781 agagcgaagg gatgagcatt cttttagcag cgccaaccgg aagggcgaca aagagaatga
      841 cagaagctac cggatatgag gcgcagacga tccatcgtct tttggaagtg aatgtaaatc
      901 ccgaagaagc ggaaaattcg ggaggatttt taagaaatcg gcagaatccc cttgaagcgg
      961 atgtgattat tattgatgaa atgtctatgg ttgatctgac attgatgcat gcgcttttgt
     1021 ctgcagttat accggggaca agactgatac ttgtaggaga cgtagaccaa cttccgtctg
     1081 tgggaccggg aagcgtattg aaagatatca ttgcatcgca tcggtttcct gttgtgacac
     1141 tgactcgtat ttttcgtcag gcaggagaaa gtgatattat agtaaatgca cataaaatca
     1201 atgcaggaga gccggttatt cccgataata agagcagaga ttttttcttc ttacgaagac
     1261 aggatgcaga tacgatcata ggtattgtga tcatgcttat ccagaaaaag cttccgaagt
     1321 atgtaggggc agatcaaaac gagatccaag ttatgacgcc gacgagaaaa ggacttcttg
     1381 gggtagaacg tttaaatgaa atccttcaga aatacttgaa tccggaagaa ccgaaaaagg
     1441 cgcagcgtga gattaacggc aggctgttca gagaaggcga taaagtaatg cagattaaaa
     1501 ataattatca aatcgagtgg gaagtatcta caaaattcgg tctgacggta gacaaaggaa
     1561 ccggtgtgtt taacggagat atgggagtga ttacagagat taacgagtat acggaaacga
     1621 tagaggtaga atttgacgaa tctcgaaaag taaaatatgg gtttgatatg acagaagaac
     1681 tggaactggc atatgcaatt accgtacata agtcacaggg gagcgaatat ccggcagtta
     1741 tcattcctct tttgccgggc ccgaagcttc tttataaccg gaatctgttg tatactgcgg
     1801 tgacaagggc aaaaaaatgt ttgacgattg tagggagtga ggcagtattt caggaaatga
     1861 tacaaaacaa aaacgaacag gcgcggtata caagtttggc ggaacgtata caagaatttt
     1921 aaatctgcta tatccgaaag tatgcccttt ctgcggggaa atttcgccgg aaggggtttg
     1981 cgcggagtgt aaaaagaaga ttatatacgt cagacagcca agatgtatga gatgtgggaa
     2041 gccgataaaa gatgaatata aagaatattg ttcggattgt gcaaaaagaa catcgcatat
     2101 tacccagggg cgaagtttgt ggcttcataa agaaccggta tcaacggcga tatataaatt
     2161 caaatataat aataagcgca gttggggaga attgtttgcg gcagaaatgg cacaagctta
     2221 taaagatcaa gttataaaat ggggaattga ggaaattatc cccattcctc tgcacaaatc
     2281 tcgaaagaga aaaagagggt ataatcaggc ggagattata gcaaaatgtt tgtcggaaaa
     2341 gacaggagtg tctctagtgt cagatgcttt gtttcgagtg aaaaaaacga cggcgcagaa
     2401 aaagttggac aggcaggaac gaatgggaaa tctaagggat gcatttgctt tgtcagaaag
     2461 atggaaacct gtggcgaatg tgcttctgat cgacgatatt tatacaacgg gagcgacagt
     2521 agagcaggca gcaaaaatat taaaaaaggc aggggcgcaa aatgtgtact ttttgactat
     2581 aagcattgga caaggtattt aaatatatga tataataatc atacatatgt aattatttac
     2641 cgtatttgga atatagtata tggatttcgg aaaatacata ggaggacggg aagacgtcat
     2701 gttagataag aggaaaatac gtttgatgac tcgggctgct atttatgaaa aaaactatgg
     2761 agaagaagat tttaagatca gcagctatta taagaaagat tattcgagtt taaatacatg
     2821 ggtcacgctt atttggatca cagtcggtta tggattggcg gttgcagtgc tgtttctcgg
     2881 ttggggagag gctgcgatgg aaggcatcac gcttgcaaag ttatttgttt ttgccgcggt
     2941 ggcgataggc gcttatgtgt cgctgctgat agcatatgga atcggtgcgg gaagctttta
     3001 taaaaataag catatcaagg caaaacagag agtaaaaaag tatctgagag acctttccag
     3061 aatcgagaag atcaacaata agaaggagat aaattaatca tgagtacatt gcttgtttgg
     3121 agagaaaagc tgcagagaat atatgcgtta tattcaattt atattcaaaa agggctgcag
     3181 tttgtgacag ggctggtttt gttttgggtt atcaactcta acataggttt tatgaagccg
     3241 gcatcttcag ttttttgcac attgggattg gcagtgatct gtacatttct tccgatgaca
     3301 attaccgttt tagcggcgac agcattgata ctcgcacatt tttatgcgtt gtcgcttccg
     3361 attgcgcttg tggcgctcgt catttttatt ttaatgtata ttttctattt tcgttttacc
     3421 ccgaaaaaag catggcttgt gttgatttcg ggacttgcgt ttagtttgaa aatcccgttt
     3481 gtaatcccga ttgcattcgg tctgcttgga tcgccggtgt ggattgttcc tgcatcatgc
     3541 ggaattatca cctattatat gacagattat gtgaaagtat ccgcggcagc gctaaaggag
     3601 gcagatgcat ccggtatagc ggctaatttg atgagtttta caaagcaagt gcttgcaagt
     3661 aaagaaatgt ggcttatggt catggcagtg acgataggaa ttcttgttgt gaattttgtc
     3721 cgtacaagag gtgtggatca ttcttggaaa attgcgtcgg cagcgggagc ggcagtaacg
     3781 gttgttgttt cagcggcagg taatattgta ttaagtacaa atatatctta tatagaaatt
     3841 atattgagcg cggttcttgg cgcggtgaca ggacttgtgc tggaacttct tttcttttcc
     3901 gtagattatt ctcgtacgga gaatatccag tttgaggatg atgaatacta ttattatgta
     3961 aaagccgttc ctaaagtcgg agtagccgtt ccggaaaaga aggtaaaacg gattactgaa
     4021 aaccagcctc aggaaacgac ggtgatcaga gaagatgcgg atttgaacaa tataaataca
     4081 gagaatgatg cggatgatat tcttttgaca agaagtttga gtaaagaact cggtttggaa
     4141 aaacaaagac cggagtaaat cgaagtaaac ggtaaggtgg tgattacggt atgttagatt
     4201 ggataaaaag tattttagac aattatacaa atggaatgta ttttcctaac atccatatta
     4261 aagatgtgat cgaaatactg atcgttgccg taatcgtgta tgagatcatg ctttggatca
     4321 agaatacaaa agcgtggatg cttcttagag gaattattat gctgggggta tttattcttt
     4381 tggcatggat ttttcagatg catacgattt tgtatttggc ggcgcagtcg atcaacgtac
     4441 tggctattgc ggctgtagtg gtgtttcagc cggaactgcg tcgtgcgctt gaaaaactcg
     4501 gagagaaaaa cctgcttagc aatattaatc cgtttgataa aaacagagag aatgaacgtt
     4561 tttctgatca gacagcagaa ggtatagtga cggcatgttt tgaaatggga aaagtatgta
     4621 caggagcctt gatcgtagta gagcaggcga ttcagcttac agaatatgaa atgaccggaa
     4681 ttgaattgga ctgtaaggtg tcacctcagg ttttgatcaa tatatttgaa cataatacac
     4741 cgcttcatga cggagcgatt attatacgag gagaccggat tacttcggct acatgttacc
     4801 ttcctctttc tgataatatg cgcatcagca aagatctcgg aacgaggcac agggcggctt
     4861 tgggaatgag tgaggtatgt gatgctcttg taatcgcggt gtcggaggaa acaggtcttg
     4921 tttccgtagc tatgggcggt cagcttgaca gagggattac aagggaagaa ttgagaaagc
     4981 gtcttagtca gattcagtat agaaatgccg agtcaaagcg gtttttcttg cggaaaggat
     5041 ggcgcagaaa atgaaaaaat ataagtttac ggataatttg ggattaaaga tcatagcggt
     5101 tatttttgcg gcgttcttat ggcttattgt tgtgaatctt gataatcccg taagtacaca
     5161 gactttcagc gaggttccgg taacgattat aaatgaggat attattttat cggcaggcga
     5221 tacctatcag gtgcttgggg aagaaaaagt aagtgttgtt gtatcggcga caagacaagt
     5281 gcggcagaaa ctgacaaaag aagatattgt cgcgacagcg gatatctgtg ggtttgaggg
     5341 cgtgagggaa cagctggatt tgt
//
LOCUS       DBMDXF010000127         5329 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3822_length_5329_cov_1.661821, whole genome shotgun sequence.
ACCESSION   DBMDXF010000127 DBMDXF010000000
VERSION     DBMDXF010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_5329_cov_1.661821"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="ACZPD4_06020"
     CDS             complement(<1..509)
                     /locus_tag="ACZPD4_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1378653.1"
                     /translation="METLIFYTYEFAASILPFSAAFIFLSHKQKKGGEKTHLLPGLLL
                     FLFAVYIVCVYHVTGTGTLWDAFLYRFQTEISHINPIPFSAGIDMVGYILNIILFIPF
                     GFLIPLIWKKMRSLHLTALAGFSFSILIEISQLLNNRRTDIDDLIMNTLGAIIGFLIY
                     KSFKKLMPT"
     gene            820..2169
                     /gene="brnQ"
                     /locus_tag="ACZPD4_06025"
     CDS             820..2169
                     /gene="brnQ"
                     /locus_tag="ACZPD4_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIR1378654.1"
                     /translation="MKKNLTFKELIFVASMLFGMFFGAGNLIFPIFMGQLAGHHVWAA
                     SAGFLITGVGLPLLGVAALGVSQKDGLLELSSQIGRKYGVFFTCLLYLTIGPFFAIPR
                     CATVSFTVGIEPFISDGGKMAGLGIFSFVFFAVVLFFSLKPGQILTWIGKILNPLFLI
                     LLSIFVIRALFSPAGNMSKIEASGAYVSGAFSTGLLEGYNTMDALAGLAFGIIVVNVI
                     RSLDIKESGAVAKNTIKAGVFSSLLMALIYVLVAVVGAQSRGVFPVAANGGETFAIVS
                     EYYFGKPGQIILALIFAVACLKTAIGLVTSCGETFEKIFPNGPSYRVWAVIFSLLSFL
                     IANVGLDAIIAYSLPVLMFLYPLAVTLILLTLCGKLFGNDRRIYSWVTAFTAAAAILD
                     FVKALPKNSDVIVKLQEMLGAVSKMLPISKYGFGWVCPALVGLMIGLALQKIRKNKR"
     gene            2352..3359
                     /locus_tag="ACZPD4_06030"
     CDS             2352..3359
                     /locus_tag="ACZPD4_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1378655.1"
                     /translation="MATISDVARLSGMSVSTVSRVINNKPHVSEEKRQKILEAMDALG
                     YSPLQAARQLRGSGSGNIAVVVPTITNNFFAQLVDSIEKTCRQYHYRTLIVQTYGEKE
                     SEESAMNLLKMQHADGVILCAIENDWDKLKSYGEYGKLVVCNEYNQDKEISMVCGRQY
                     EGFYKASKYLLEKGYRKIAYCTGTRAVTLQDKGINIDSDRYRGYMDALAQSGIAANTA
                     WLFSGVREMEDGRKIMRKILELSEKPDAVIAGSDEVAAGMVMEALKNGVKIPQDIAIM
                     GVDDQPLASYLQIPLTTIWQPVQDEGRCAAKEMVRQLKGESEGIRRKELDLKLIVRRS
                     A"
     gene            3505..3963
                     /locus_tag="ACZPD4_06035"
     CDS             3505..3963
                     /locus_tag="ACZPD4_06035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1378656.1"
                     /translation="MVVLARIDQRLIHGIIVNQWAPALQVKRFMVVDDILCSNEEVKA
                     SMRMAKPAGTGVSVISAETAIANFKAGKYDGQRVFVLVKEPETLIRLIEGGVEIPKVD
                     LGIMFNEDGRTPISKFIALNEKEKEDLETIKGKGIPVVIQYVPTDAEEAY"
     gene            4052..4417
                     /locus_tag="ACZPD4_06040"
     CDS             4052..4417
                     /locus_tag="ACZPD4_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1378657.1"
                     /translation="MEVIQNILIVILAGYMTVDQNGPVVLSWFSVIVGMISGLIMGDM
                     NTGLMIGGTFQLMSLGVAALGGASAPNYGLATIIGTFIAVRTGTGVDAAIAVGLPVGL
                     LAIQLEVVVRIHINFISDF"
     gene            complement(4601..>5329)
                     /locus_tag="ACZPD4_06045"
     CDS             complement(4601..>5329)
                     /locus_tag="ACZPD4_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HIR1378658.1"
                     /translation="KVELSLNSQGEDNTAPTISANDVTLNVGDTFDPLANVTATDKED
                     GTITLTKDNIVANDVDTSKAGTYHVTYKVTDKNGASAKKTITVTVKQNTGDLNSAPII
                     SANDVTLNVGDTFDPLVNATATDKEDGTIILTKDNIIANDVDTSKAGTYHVTFRVVDK
                     NGAITEKTITVTVKEKATNKPVSPQQPQKPSKPTTPTKPSGQKNPVKTGDMTNVGLFT
                     SMFAGSTGVLAVLFGKKRKRNKNN"
BASE COUNT         1607 a          820 c         1304 g         1598 t
ORIGIN      
        1 ctggttggca tcagtttctt gaatgattta tatattaaga atccgataat cgctcctaaa
       61 gtattcataa taaggtcgtc gatatcggtt cgtcggttat ttaataactg gcttatttcg
      121 atcagtatgg aaaaagaaaa tcctgcaagg gcggttaggt gcagcgatct catttttttc
      181 cagattaaag ggatcaaaaa cccaaacgga atgaacagga tgatattcaa aatatatccg
      241 accatatcga ttcccgcaga aaaggggata ggattaatgt gggaaatttc tgtttggaag
      301 cgatacaaaa atgcatccca taaagtgccg gttcctgtga cgtgatatac acatacgatg
      361 tagacggcaa aaagaaataa aagcaggccg ggcagaagat gtgttttttc tccgcccttt
      421 ttctgtttgt gcgaaagaaa aataaaggca gccgaaaaag gaaggatcga agccgcaaat
      481 tcataggtat aaaaaattaa agtttccata gagtttctcc tttatatatg ttttaaaagg
      541 ctgttgggag ttgtgaaaaa gcaagttcgc ctaagtgttt gtgtttcgtt aaaagtataa
      601 aataagtata tcatcgaaaa taagaagatt gtgggaattg cgggaatatt aaggaatatt
      661 aaggaaagct taaataaaac tggcgaatcg gaagtgtata tgatacactg gatgtattct
      721 tgataaaaaa tgtacagtaa aggcgtttgc cgcatcggaa cgaaatagat gtaatgggag
      781 aaatatttat agtattcaga cgtataaggc aggtggaaca tgaaaaagaa tcttacattt
      841 aaagagttga tatttgttgc aagtatgttg tttgggatgt tttttggcgc gggaaatttg
      901 atctttccga tatttatggg acagcttgca ggccatcatg tgtgggcggc atcggcaggt
      961 tttctgatca caggtgtcgg gcttccgctt cttggtgtgg cggcgcttgg cgtcagccag
     1021 aaagacggac ttttggaatt aagcagtcag atcggcagaa aatacggagt tttctttacg
     1081 tgtcttttgt atttgacgat cgggcctttt tttgcaatcc caagatgcgc tacggtttcg
     1141 tttacagttg gaatagagcc gtttatttca gacggcggga agatggcagg attgggaata
     1201 ttctcttttg tattttttgc cgttgtattg tttttttcat tgaagccggg acagatctta
     1261 acgtggatcg ggaaaatact gaatcctctg tttttaattt tgttgagtat ttttgttatt
     1321 cgggcgcttt tttcaccggc cggaaatatg agtaagatcg aggcatccgg cgcatatgtg
     1381 tccggcgcct tttcaaccgg tctgctggaa gggtataata cgatggatgc tctggccggg
     1441 cttgctttcg gaattattgt tgttaatgta atcagaagcc ttgatataaa ggagtcggga
     1501 gcagtggcaa aaaatacgat aaaagcaggc gttttcagct cattattaat ggcgcttatc
     1561 tatgttttgg ttgcggttgt cggtgcgcag agccgcgggg tgtttcctgt ggcggcaaat
     1621 ggaggagaga catttgcgat cgtttccgaa tactatttcg gaaagccggg tcagattata
     1681 ttagcgctga tttttgcggt ggcatgtttg aaaacagcga tcggccttgt tacaagctgt
     1741 ggggagacgt ttgaaaagat atttccgaac ggaccttctt atcgtgtgtg ggcagtgatt
     1801 ttttctcttc tttctttctt gatcgcaaat gtaggacttg atgcgatcat tgcatattct
     1861 cttcctgttt tgatgttctt gtatccgctg gcagtgacac ttattttgct tactttatgc
     1921 ggaaagctgt ttgggaatga cagaagaata tacagttggg tgacagcgtt tacggctgca
     1981 gcggcgattt tagattttgt gaaagctttg ccaaaaaatt cggatgtaat cgtgaaactt
     2041 caggagatgc tcggtgcggt atcaaagatg cttccgatat cgaagtatgg ttttggatgg
     2101 gtgtgtccgg ctttggttgg attgatgatc ggtctggccc ttcaaaagat acgaaagaat
     2161 aaaagataag ttttatgaaa gataaaaaat caaaaaccta ttcgttaaaa ctaccaaatt
     2221 tcatttgtgg attttggatg ttttgacaaa tatgaaaacg ataccaaaaa tgcgattgac
     2281 aaatgacgaa agtgagtata ttgtaaagac ataaacaaga aaacaaagag aaaagtgagg
     2341 tggaaaaagt tttggcaacg atatcagatg ttgctcggct gtcaggaatg tcagtttcaa
     2401 ctgtctcaag agttatcaat aacaaacctc atgtttcgga ggaaaagaga cagaagatac
     2461 tggaggctat ggacgctctt gggtatagtc cgcttcaggc ggcaagacag ttaagaggtt
     2521 cgggctccgg gaatatagcg gtagttgttc ctactatcac aaataacttt ttcgcacaac
     2581 tggtcgattc aattgagaaa acttgcagac agtatcatta ccgaacattg atcgtgcaga
     2641 cttacggaga aaaagaaagt gaagaaagcg caatgaatct tctgaagatg cagcatgctg
     2701 acggagtgat tttatgtgcg atagagaatg actgggataa gttgaaatca tatggcgaat
     2761 atggaaaact tgtcgtctgc aatgaatata atcaggacaa ggaaatttcg atggtatgcg
     2821 gcagacagta tgaagggttt tataaagctt cgaaatatct tttggaaaaa ggatatcgaa
     2881 agattgcata ttgtacagga acaagagcag tgacccttca ggataaagga attaatatcg
     2941 acagcgatcg ctatagagga tatatggacg cgcttgctca aagcggaatt gcggcaaata
     3001 cggcatggct gttttcggga gtgagggaaa tggaagacgg gcgaaagatt atgcggaaga
     3061 ttttggaact ttccgaaaaa ccggatgcgg taattgccgg aagtgatgag gttgcggcag
     3121 gcatggtgat ggaagcgctg aagaatggtg taaaaatacc acaggatatt gcgattatgg
     3181 gagtagatga tcagccattg gcatcctatc tccagattcc gttaacaacg atatggcagc
     3241 ctgtacagga tgaaggccga tgtgcggcaa aagaaatggt tcgtcaatta aaaggtgaat
     3301 cagaaggaat aagaagaaaa gagttggatt tgaagctgat agtcagacgg tcggcataaa
     3361 tcggacagaa accggtatga aaatagaacc ggtttttaaa aacaaaaaat tggtaacgat
     3421 accaaacgaa aaacagagac gtatttgtaa acgaaaaaca caaataaaga aactaaaaat
     3481 gattaaaaaa gaggaggaac aaaaatggta gtattagcaa gaattgatca gcgtttgatt
     3541 catgggatta tcgtaaatca atgggcgccg gcgcttcagg tgaagagatt tatggttgta
     3601 gacgacattc tttgcagcaa tgaagaggta aaagcgagta tgagaatggc gaagccggca
     3661 ggaacgggag tatcggtgat ttcggcagag acggcgattg ctaattttaa agcagggaaa
     3721 tatgacggtc aaagagtttt tgtccttgta aaagaaccgg aaactttaat ccgtctgatt
     3781 gaaggcggcg tagagatacc gaaagttgat cttgggatta tgtttaatga agacggaaga
     3841 actcctattt ctaaatttat tgcattaaat gaaaaagaaa aagaggatct tgaaacgatc
     3901 aagggaaaag gaataccggt tgtgattcaa tatgttccga cagatgcaga agaggcatat
     3961 taagaaacaa gagattcgga taaaagagat atgtttaatg tgatgagatt tttcggaatc
     4021 ggattgttaa agagagattg aggtgaaaga tatggaagta atacaaaata ttttgatcgt
     4081 gattttggca ggatatatga cggttgacca gaacggaccg gttgttttgt catggttttc
     4141 ggtaattgta ggaatgatat caggtttgat catgggcgat atgaatacag gtcttatgat
     4201 cggaggaaca ttccagttga tgtcgcttgg cgttgcagct ttgggaggag catctgctcc
     4261 gaattatggt ttggcgacga ttataggaac gtttattgcc gtgagaaccg gaacaggagt
     4321 cgatgcagcg attgcagtcg gacttccggt agggcttctg gcaatccagt tggaagtagt
     4381 tgtaagaatt cacataaatt ttatatctga tttttgatta tccgcataaa cactatactt
     4441 tcaagcattt tgaaatatag gaaagcactc attttaccac gaatttacca cgattgaatg
     4501 agccttgata tgacgtaaga atttgaaaag gacaccgtat agtttggtgt ccttttacat
     4561 gaaaatcatt agtcgctatt gattgttatt atacattttg ttaattgttc ttatttcttt
     4621 tacgtttttt accgaataaa acagctaata caccggttga accagcaaac atagaagtaa
     4681 acaatcctac gtttgtcata tcacctgttt ttacagggtt cttttgtccg ctaggctttg
     4741 ttggagtagt tggcttgctc ggtttctgtg gctgttgtgg acttacaggt ttatttgttg
     4801 ctttttcttt tacggtaact gtaattgttt tttcagtaat tgcaccattt ttatctacta
     4861 cacggaaagt tacatgataa gtgccagcct tagaagtatc tacatcattt gcaataatgt
     4921 tatcttttgt taaaataatc gttccgtcct ctttatctgt tgctgtcgca tttaccaatg
     4981 ggtcgaacgt atcgcctaca ttcaatgtaa catcatttgc tgaaatgatt ggagcagagt
     5041 taaggtcgcc tgtattttgt ttcacagtta cagtaatcgt ttttttagca gaagctccgt
     5101 ttttatctgt tactttataa gtcacatggt aagtaccagc cttagaagta tctacatcat
     5161 ttgcaacaat attatctttt gtcaaagtga tagttccgtc ctctttatct gttgctgtta
     5221 catttgccaa tgggtcgaaa gtatcgccta cattcaatgt aacatcattt gcagaaatag
     5281 ttggtgctgt attgtcttct ccttgtgagt ttaacgataa ttccacttt
//
LOCUS       DBMDXF010000128         5325 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3825_length_5325_cov_1.690467, whole genome shotgun sequence.
ACCESSION   DBMDXF010000128 DBMDXF010000000
VERSION     DBMDXF010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_5325_cov_1.690467"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(140..1609)
                     /locus_tag="ACZPD4_06050"
     CDS             complement(140..1609)
                     /locus_tag="ACZPD4_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1378659.1"
                     /translation="MRIGFDNEKYLKMQSEHIRNRIGKFGNKLYLEFGGKLFDDYHAS
                     RVLPGFAPDSKLRLLKELSSQAEIVIVISAKDIEKNKIRGDLGITYDTDVLRLIQTYK
                     DQGLYVGSVTITQFSGQESALLFKNRLEKLHIPVYLHYLIPGYPSNIPLIISDEGYGK
                     NDYIKTSRPLVIVTAPGPGSGKMAACLSQLYHEHKRGVLAGYAKFETFPIWNLPLKHP
                     VNLAYEAATADLNDINMIDPFHLEAYGETTVNYNRDVEIFPVLNTIFEKIYGESPYKS
                     PTDMGVNMAGNCICDDEACREASKQEIIRRYFDALNALAKGSTSEKEAQKIELLMNMA
                     GITVTDRKVVIKALERAKETDGPAAALELRDGRIITGKTTNLLGASAALLLNVLKVLA
                     GIDHETHIISPESIEPIQKLKVDYLKSKNPRLHTDEVLIALSASAATNPQARLALSQL
                     PELAGCQAHTSVMLSDVDIKIFKKLEVQLTSEAVYEHAL"
     gene            1818..3845
                     /gene="ftsH"
                     /locus_tag="ACZPD4_06055"
     CDS             1818..3845
                     /gene="ftsH"
                     /locus_tag="ACZPD4_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1378660.1"
                     /translation="MDNNQNKKNNKNNPNNNRQGLGIVLITTLLVAFVLIGFFSAMGN
                     TSQREISYDKFLKLVSEHKVKEVKLDSEKIYITLTDKAREEAREKAVEKAEKEGRLSP
                     FEEAMERLRIQLPSVKKEGKEHAPDFYTGYVDDDTLVTKLDRAGVKFQSEIPDTIGQL
                     FFEMFVTVILPLLLMVWLFTYMMKRMNKGGGMMGIGKSNAKMYMEKETGVTFRDVAGE
                     DEAKESLQEVVDFLHNPGKYSGIGAKLPKGALLVGPPGTGKTLLAKAVAGEAKVPFFS
                     LSGSAFVEMYVGVGASRVRDLFKQAQQSAPCIVFIDEIDAIGKTRDTAMGGNDEREQT
                     LNQLLAEMDGFDTNKGLLILAATNRPEILDPALLRPGRFDRRIIVDKPDLKGRVDILK
                     VHAKDVRMDESVDLEAIALATSGAVGSDLANMINEAAINAVKHGRQVVSQKDLFEAVE
                     VVLVGKEKKDRIMSEEERKIVSYHEVGHALVTALQKNTEPVQKITIVPRTMGALGYVM
                     QTPEEEKFLNTKKELEAMIVVALGGRAAEEIVFDTVTTGASNDIEQATKIARAMITQY
                     GMSDRFGLMGLESIQNKYLDGRAVLNCGEATAGEIDEEVMKMLKSAYAEAKKLLSENR
                     EALDKIAEFLIEKETITGKEFMKIFREVKGISEPEEGAVSGIEERGRIAMK"
     gene            3989..4507
                     /locus_tag="ACZPD4_06060"
     CDS             3989..4507
                     /locus_tag="ACZPD4_06060"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1378661.1"
                     /translation="MEYRNSYISPLGRILLTADEIGLTGLAFEGQKNAVQGLIRESEE
                     KETTLFSGVKKWLDIYFSGVEPDFQIPIHLEGTDFQMCVWEMLCTIPYGETTTYGAIA
                     KQIAHDRGISKMSSQAVGNAIGRNPVSVIVPCHRVIGAKGNLTGYAGGIERKAELLKI
                     EQNQNATEQYPF"
     gene            4581..5231
                     /locus_tag="ACZPD4_06065"
                     /pseudo
     CDS             4581..5231
                     /locus_tag="ACZPD4_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
BASE COUNT         1587 a          934 c         1384 g         1420 t
ORIGIN      
        1 tacgaaaaaa ttatagagga actttttgcg aatgaggaag taatatagaa atacaaaaaa
       61 cagccggatc taaaaacatc cggctgtctg taacataagg ataataagag gggaggagta
      121 atccttcccc tttgttattt tataaagcgt gctcgtatac ggcttcggaa gtgagctgca
      181 cttccagttt cttaaagatt ttaatgtcta cgtctgacag catgacagat gtgtgcgcct
      241 ggcatcctgc aagttcggga agctgggaaa gggcaaggcg tgcctgcgga tttgttgccg
      301 cgcttgcgga tagggcgata agtacttcat ctgtatgaag ccgtggattt ttgctcttta
      361 aataatctac ttttaatttt tggatcggtt cgatcgattc aggagaaata atatgagttt
      421 cgtgatcgat acctgcaagc acttttagta catttaacag aagcgccgcg cttgctccga
      481 gaagattggt tgtttttccg gtgatgatcc ttccgtcacg cagttcaaga gcggcagcgg
      541 gaccgtctgt ctctttggcg cgctctaaag ctttgattac aactttgcgg tctgtgacag
      601 taatccctgc catattcata agaagttcga ttttctgagc ttctttctcg gaagtgctgc
      661 cctttgcaag agcgttcaga gcatcaaaat atcggcggat gatctcttgt ttggaagctt
      721 cacggcaggc ttcgtcgtcg cagatgcagt ttcctgccat gttgacgccc atatcagtag
      781 gagacttgta aggactttct ccgtaaatct tttcaaagat tgtatttaat acagggaaaa
      841 tctcaacatc acggttatag ttgacagtcg tttctccata cgcttcaaga tggaacgggt
      901 cgatcatgtt tatgtcgttt aagtcggcag ttgccgcttc gtatgcaagg ttaaccggat
      961 gcttaagcgg gagattccat atagggaacg tctcgaattt tgcatatccc gcaagaacgc
     1021 cccgtttatg ctcgtgataa agctgggaaa gacatgctgc catcttgccg cttccgggtc
     1081 cgggagcagt gacgataaca agaggtctgc ttgtcttgat ataatcattt tttccataac
     1141 cttcgtcgct gatgataagc ggaatattag atggatatcc gggaataaga taatgaagat
     1201 ataccggaat atgcagcttt tcaagtctgt ttttgaacag gagggcactt tcctgaccgg
     1261 aaaattgagt gattgtaaca cttcctacat ataatccctg atccttgtat gtctgaatca
     1321 gacggagaac atctgtgtca taggtgatgc ccagatctcc gcggatttta tttttttcaa
     1381 tgtctttggc gctgatgacg ataacgatct ctgcctgaga actcaactct tttaaaagac
     1441 ggagtttact gtcaggagca aatcccggaa gaacacggga cgcatgataa tcatcaaaca
     1501 gtttgcctcc gaattcaaga taaagcttgt tgccaaattt tccgatccgg tttcggatat
     1561 gttcggactg cattttcaga tatttttcat tgtcaaaacc gattctcatt ttattcttct
     1621 ccactttttt gttcatttta atcctgcatg caaaagaata gcggcaggac acatttcgta
     1681 ctcagtatac tataaaatct aactgtttta caatattttc ataatctttt tattgattta
     1741 tgcaaaagct gtacttataa tggtaataac gtcatgcacg ttgtgcaatg acgccgaaga
     1801 tttttaggag gaacaggatg gataacaacc agaataagaa gaataataaa aataatccga
     1861 ataataaccg gcagggtttg ggcattgtgc ttataacgac cctgctggtg gcttttgtat
     1921 tgatcggatt cttttctgct atgggcaata caagtcaaag ggagatcagt tatgacaaat
     1981 ttcttaaact tgtaagtgaa cataaagtaa aagaggtaaa gttagacagt gaaaagattt
     2041 atattacatt aacggacaaa gcccgtgagg aagcgcggga aaaagctgtt gagaaagcgg
     2101 aaaaagaagg acgcctcagt ccgtttgaag aagcgatgga aagattgcgt atccaactgc
     2161 cttccgtaaa aaaagaaggg aaagaacacg cgcctgattt ttatacggga tatgttgacg
     2221 atgatacgct tgtgacaaaa cttgaccgtg ccggggttaa gtttcaaagt gagattccgg
     2281 atacgatcgg tcagttgttt tttgaaatgt ttgtcacggt gatcctgccg cttctgctca
     2341 tggtgtggct gttcacatat atgatgaagc gcatgaataa gggcggcgga atgatgggca
     2401 ttggaaagag caatgcgaag atgtacatgg aaaaagaaac aggagtgacg ttccgggatg
     2461 tggcaggaga agatgaagcg aaagaatcgc ttcaggaagt cgttgatttc cttcataatc
     2521 cgggaaaata cagtgggatc ggagcgaaac ttccgaaagg agcgcttctt gtaggaccgc
     2581 cgggaacagg aaagacactt ttggcaaagg cggttgcggg agaggcaaaa gttccgtttt
     2641 tctcattatc gggttctgcg tttgtggaaa tgtatgtcgg tgtcggagca tcccgtgttc
     2701 gtgatctgtt caaacaggct cagcagtcgg ctccgtgtat tgtgtttatc gacgagatcg
     2761 atgcgatcgg aaagacaaga gatacggcga tgggcggaaa tgatgagaga gagcagactc
     2821 tgaatcagct tttggcggag atggacggat ttgatacgaa taagggactt ttgattctgg
     2881 cggcaacaaa ccgtccggag attcttgatc ctgcgcttct tcgtccggga cggtttgaca
     2941 gacggatcat tgttgataag ccggatctga aaggccgtgt cgatatttta aaagtacatg
     3001 caaaagacgt tcggatggat gagagcgtgg acttggaagc aatcgcgctt gcaacatccg
     3061 gagcagttgg ttcggacctt gcgaatatga tcaatgaagc ggcgatcaat gcagtaaaac
     3121 acggaaggca ggttgtgagt caaaaagatt tgtttgaggc ggttgaagtt gttcttgtcg
     3181 gaaaagagaa gaaagaccgg atcatgagcg aggaagagcg gaaaattgtt tcttatcatg
     3241 aggtaggtca tgcccttgtg acagcgcttc agaaaaatac agagccggta cagaagatca
     3301 cgatcgttcc gagaacgatg ggcgcgctgg ggtatgtgat gcagactccg gaagaagaga
     3361 agttcttaaa tacaaagaaa gaactggaag cgatgatcgt tgttgcgctc ggaggacgtg
     3421 cggcggaaga aatcgtgttt gatacagtaa cgacaggcgc gtcaaatgat attgaacagg
     3481 caacgaaaat cgcccgtgcc atgatcacgc agtatggaat gtcagacagg ttcggactga
     3541 tggggcttga atcgatccag aacaaatatt tggacggccg tgcggttctt aactgtggag
     3601 aggcgacagc gggagagatt gacgaagaag taatgaagat gctgaaatct gcgtacgcag
     3661 aggcgaagaa acttctttcc gaaaacagag aagctcttga taaaatcgcg gaatttctca
     3721 ttgaaaaaga aacgatcaca ggaaaagaat ttatgaagat attcagagaa gtaaaaggaa
     3781 tttccgagcc tgaagaaggc gcggtatccg gtatagaaga gcgtgggaga attgcgatga
     3841 aataaggaaa taagggttgt tttccgtttg agagaaagcg gagagcgatc ctttttcttt
     3901 tgaaatcata aaatccgtcg aatctttcgg aattttggaa gaaaagaatt tccgggaacg
     3961 gatagtttga gaataagagg gaaaatggat ggaatacaga aacagttata tatcgccgct
     4021 tggcaggata ttgctgacag cggatgagat cggactgacc ggactggcgt ttgaaggaca
     4081 gaaaaatgct gttcaaggtt tgattcggga aagtgaggag aaagagacaa cacttttttc
     4141 gggtgtgaaa aaatggctgg atatatattt ttcaggagtg gaaccggact ttcaaatacc
     4201 gattcatttg gaaggaaccg attttcagat gtgtgtgtgg gagatgcttt gcacgatccc
     4261 gtatggagag acaacgacat acggcgcaat cgcaaagcag atcgcacatg acagagggat
     4321 ttcaaagatg tcgagtcagg cggttggaaa tgctatcgga cgcaaccctg tgtcggtgat
     4381 cgttccgtgt cacagagtga tcggagcaaa aggaaatctg acaggatatg caggcggaat
     4441 tgagagaaaa gcagagcttt taaaaataga acaaaaccaa aatgccactg agcagtatcc
     4501 cttttgataa agtgaaagag aatgtttata aaagataagt ttttcttgtt tttttatata
     4561 tgggaagtat aataaagttt atggaaaaca ataattttaa catacaggaa gaattaaaaa
     4621 agcttcccgc aagaccgggc gtatatctta tgcatgacga gcgggacgag atcatttatg
     4681 tcgggaaagc gatcagttta aaaaacagag tgcggcagta ttttcaaagc agcagaaata
     4741 aaggcgtcaa gatagaacag atggtgacgc atatcaggcg ttttgagtat attgtgacag
     4801 attcggagtt ggaggctctt gtattagagt gtaatctgat caaagaacat catccaaaat
     4861 acaatacgat gctgatggat gataaaacat atccatttat caaagtgacg gtgaatgaac
     4921 cctttccgag agttttgctg gcaaggaaaa tgctgaaaga taaggcaaaa tacttcggtc
     4981 cgtacacgag tgcgcaggcg gttagagata cgattgatct gatccataaa ttgtaccatg
     5041 taaggagctg caatcgaaat cttccaaaag atatcgggaa agaacgtcct tgtcttaatt
     5101 atcatatcaa gcagtgcgac gctccgtgtc agggctatat ttctcaggaa gactacgggg
     5161 cggcgatcgt ggaagtgttg aagtttttga atggaaatta tgatgtgatc ttgaaagagt
     5221 tggaaggaaa gtttatggta attggtctgg atggtcagta agtatatctg ctagtcctac
     5281 taatattgct gctgctggcg gtagttctac tattacttgt agtgc
//
LOCUS       DBMDXF010000129         5298 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3848_length_5298_cov_1.965172, whole genome shotgun sequence.
ACCESSION   DBMDXF010000129 DBMDXF010000000
VERSION     DBMDXF010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3848_length_5298_cov_1.965172"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="ACZPD4_06070"
     CDS             complement(<1..538)
                     /locus_tag="ACZPD4_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1378662.1"
                     /translation="MSRQIWKPGNMLYPLPAVMVSTADKEGNSNIITIAWTGTVCTNP
                     AMVYISVRPERYSYHMIKESGEFVINLTTEKLTKATDYCGVRSGENVDKWKECRLTQG
                     KASSLEFAPIIEESPVNIECKVKKMEELGSHHMFLAEVTAVQVDDSYMDDNGKFMLNN
                     TGLIAYSHGEYLSLGEKLG"
     gene            complement(567..2213)
                     /locus_tag="ACZPD4_06075"
     CDS             complement(567..2213)
                     /locus_tag="ACZPD4_06075"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIR1378663.1"
                     /translation="MSRQKDKIYVVGHKNPDTDSICSAIAYAALKTKLTGHAYEPRRA
                     GQLNEETQYVLEKFAVKPPKLLSDLREQIKDVELKEVEGLTSNSSIRTAWETMRELNI
                     HTLPVVRDGSLEGVITIGDIAKTYMDVYDSMILSKARTQYRNIARAVDGEVITGNEHS
                     YLLNGKVVIAASSRILMSDFISRDDLVIMGDRKDAQQCAVDMEASCMVVCQNASISEE
                     IIKQAEKKQIVIIRTPHDTFTAAQHINQSIPVKYFMTKTNLVTFKMKDYVDDVKEVMA
                     RKRFRDFPIVDGNGKLLGLISRRRLLNVRKKQVILVDHNEKSQAVDGVEEAEVMEIID
                     HHRLGSIQTMGPVFFRNQPVGCTATIVSQMYEENGVEIDPTNAALLCSAIISDTLMFR
                     SPTCTPLDENTARKLAQTAGVEIEILAQEMFRAGSNLKGKSAQDICFLDFKKFTVNET
                     VFGVGQVNSMSSDELEGIKKKVKPYLEKACADSGVNMIFFMLTNILTESSELLCGGPE
                     AKEKILNAYDLPEDTDTIMLKGVVSRKKQLVPTLVSVLQQ"
     gene            2470..3189
                     /locus_tag="ACZPD4_06080"
     CDS             2470..3189
                     /locus_tag="ACZPD4_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1378664.1"
                     /translation="MSEEMKEPMQTDNDCKTEEAAASTTETMADYEDHFDDANPWNLV
                     TKYMEEKTVLPVKVEGVVNGGVIVMIEGLRGFVPASKLSLSYIEDLETYLLKEIEVQV
                     IDADQANNRLVLSARELLKEKEKKAREDQIANLKIGEVLEGTVETLQNYGAFIKLDNG
                     LSGLVHVSQISQKRVKLPSDVLNAGDKVTVKVIGIKDGKISLSMKALEEVKEEPTEKV
                     VIPKSENIGTNLGDLFKNIQL"
     gene            complement(3337..3624)
                     /locus_tag="ACZPD4_06085"
     CDS             complement(3337..3624)
                     /locus_tag="ACZPD4_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HIR1378665.1"
                     /translation="MSVVIIGGHDRMVCQYKQICKQFKCKAKVFTQMSAAMSKQIGSP
                     DLIVLFTNTVSHKMVRCAVEEAGRCNAEVVRCHTSSKNALEEILGNICVQG"
     gene            complement(3823..5196)
                     /locus_tag="ACZPD4_06090"
     CDS             complement(3823..5196)
                     /locus_tag="ACZPD4_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1378666.1"
                     /translation="MVKTKDAVNDMTVGTPAKLIIQFMIPVFLGNIFQQFYNIADSIV
                     AGQFIGVKALAAIGSTGSLMFFVTGWLNGLSSGFAILVSQWFGAKQYDKMRHYVAMSV
                     YLAGGCAVLMTAGLLIANEPILRMMNYSAEIMPEVKQYMGIIYAGLIVTAAYNSLAAF
                     LRAIGDSKSPLYFLIISAVINVALDVVFIVVFGMGVEGCAYATVIAQAISALLCFIYI
                     VKKFPILHLTKSDRKISFQSFGRLLSLGIPMGLQFSITAIGTIIVQGAVNVYGETYMA
                     GFSAAGKLQNMIMTVFVAFGATVATYVGQNRGAGRMDRVKSGVRCTQVMILVWSVIAM
                     AAVCFGGKYMTWLFIDPSETEVVNVSVLYFRTVFWCYPFLGSIFLYRNTLQGMGYGLV
                     PMLGGVFELAARSAIVMAAAGRTTFQGVCLADPAAWIAALIPLVPYYFYTMRKWKTTE
                     TKIQKQI"
BASE COUNT         1523 a         1309 c          866 g         1600 t
ORIGIN      
        1 gaccaagctt ctctcccaga gataaatatt ccccgtgaga ataggcaatc aatcccgtat
       61 tattcagcat aaattttccg ttatcatcca tatatgaatc atccacctga actgcagtca
      121 cttctgcaag gaacatatga tgacttccaa gctcctccat tttttttact ttacattcaa
      181 tatttaccgg gctttcttct ataataggag caaattcaag agatgatgct tttccttgcg
      241 taagacgaca ttctttccac ttatctacat tctctcccga acgaactcca cagtaatctg
      301 tcgcttttgt caatttttct gtcgtcagat tgatcacaaa ctccccgctt tcttttatca
      361 tatgataaga atagcgctcc ggacgaacag aaatatatac catcgccgga ttcgtacata
      421 cggttcccgt ccatgcaatc gtaattatgt tactgtttcc ttctttatct gcggtactga
      481 ccatcaccgc cggaagcgga taaagcatgt ttcccggttt ccatatttgt ctggacattt
      541 tatccttcct ctctttttcc gttttattat tgctgcagga cgctgacaag cgtcggaaca
      601 agctgctttt tccttgatac tactcctttt agcatgatcg tatccgtgtc ttccggcaaa
      661 tcatatgcat ttaatatttt ttcctttgcc tccggaccgc cgcaaagaag ttccgatgat
      721 tctgtcaaaa tatttgtcaa cataaaaaag atcatattta ccccgctgtc tgcgcacgct
      781 ttctccaaat acggctttac tttctttttg attccttcca gttcgtcgga actcattgaa
      841 ttcacctgtc ctactccgaa cacagtctcg ttgaccgtaa attttttaaa atcaagaaag
      901 catatatcct gagcgctttt ccctttcaaa ttacttcctg cacgaaacat ttcctgcgcc
      961 agaatttcaa tctcgacgcc cgcagtctga gccagtttcc tcgctgtgtt ttcatccaaa
     1021 ggcgtacagg tcggagagcg gaacatcaac gtatcggaaa taatagcgga acaaaggagc
     1081 gcggcgtttg tcggatctat ttcaactccg ttttcttcat acatctgact gacgatcgtc
     1141 gcagtgcatc ccaccggctg attacggaaa aatacaggtc ccattgtctg aatactgccc
     1201 agtctgtggt gatcgatgat ctccattact tctgcctctt ccactccgtc cacagcctgg
     1261 cttttctcat tatgatcgac aagaatcact tgtttttttc ttacgttcaa taaccgtcgt
     1321 cttgagatca gtcctaataa tttcccgttt ccgtcaacaa tcgggaaatc tctgaaccgt
     1381 tttcgcgcca tcacttcttt tacgtcatca acataatcct tcattttgaa tgtaaccaga
     1441 ttcgtttttg tcataaaata tttgaccgga atgctctgat tgatatgctg cgccgcagta
     1501 aaagtatcat gaggcgtcct tatgatcaca atctgctttt tttccgcctg tttaataatc
     1561 tcttctgaaa tcgaagcgtt ttgacagacg accatacagc ttgcttccat atctaccgca
     1621 cactgctgtg catcctttct gtctcccatg atcacaaggt catctctgct tataaaatcc
     1681 gacatgagaa tcctgcttga agctgcgatc actacttttc cgtttaacag atagctatgc
     1741 tcatttccgg tgatcacttc cccatctact gcacgggcaa tatttctata ctgagtcctt
     1801 gcctttgata aaatcatact atcataaaca tccatatatg tcttggcaat atcaccgatc
     1861 gtaataaccc cttccaaact gccgtctctt acaaccggaa gtgtatgaat gttcagctcc
     1921 cgcatcgttt cccacgctgt gcggattgaa gagttacttg tcaatccttc tacttctttc
     1981 aactcaacat ctttgatctg ttctcttaaa tcagacaaaa gcttcggagg tttcactgcg
     2041 aatttttcaa gaacatactg tgtttcttca tttaattgtc cggctctgcg cggctcataa
     2101 gcgtgacctg tcaattttgt ttttaatgcg gcgtatgcaa tcgcggaaca tatggaatct
     2161 gtatccggat ttttatggcc gacaacatat attttatctt tctgtctact catatatttt
     2221 tactctcgct ttcttcatcc aacatcaata atctgaaaag attctatgct tatcgataaa
     2281 aacatttttc atccataact gcattattat atacataaat ctcattcttc tgcaagagca
     2341 caataaacaa tatagtcaag aaaaattaag aataaaaata ccattcttct ctactttcaa
     2401 aatattgttt tttctcctcc atctgtggta tactctttct atataaaaaa gaaacagagg
     2461 tgctgcacaa tgagcgaaga aatgaaagaa ccaatgcaga cagataatga ctgcaaaaca
     2521 gaggaagctg ctgcttccac aacagaaacg atggctgact acgaagatca tttcgacgat
     2581 gccaatccgt ggaatctcgt cacaaaatat atggaagaaa aaacagttct tcctgtaaaa
     2641 gtagaaggcg ttgtaaatgg cggagtaatc gtgatgatcg aaggactgcg tggatttgtt
     2701 cctgcttcta aactttccct ttcctatatc gaagatctgg aaacttatct tctgaaagaa
     2761 atagaagtac aggtaattga cgctgaccag gcaaataacc gtctcgtcct ttccgcaaga
     2821 gaacttttaa aagaaaaaga aaagaaagcg cgggaagatc agattgccaa cctgaaaatc
     2881 ggagaagttt tagaaggaac agtcgagact ttacaaaatt acggcgcgtt catcaaactt
     2941 gacaacggtc tgtccggtct tgtacacgtt tcacagattt cacaaaagcg tgtaaaattg
     3001 ccttccgatg ttttaaatgc cggagacaaa gtcactgtaa aagtaatcgg aatcaaagac
     3061 ggaaaaataa gccttagtat gaaagcgctt gaagaagtaa aagaagaacc gacagaaaaa
     3121 gttgttatcc cgaaaagtga aaatatcgga acaaatctcg gtgatctttt caaaaatatt
     3181 caattataaa tcatatctct tatttacaac ggtacaggcg gcaaagaatt tgtttgaaaa
     3241 attctttgcc gcctgtaccg ttgtattgcc ataaccttgt tttctttaca gcttccgcct
     3301 ttctttctga aatatctcca cggacatcta aaaagctcac ccctgcacac atatattccc
     3361 cagtatctct tccaatgcgt ttttactgct tgtatggcat ctgacaacct ccgcattgca
     3421 tcgtccggct tcttctaccg cacagcgtac cattttgtgt gataccgtgt tcgtaaaaag
     3481 aacgatcaga tccgggcttc ctatctgctt actcatcgcc gccgacatct gagtaaacac
     3541 tttcgcctta cacttaaact gtttacatat ctgtttgtac tgacatacca ttcggtcatg
     3601 tccgcctatg atcacaacac tcatcgccaa cctcctgaaa tatttttctg aatccttcac
     3661 gtatgatctc cattcacaga aaatactatg acacaacatt gcttctgttt tttggttagt
     3721 tgcggctaac tactttcata tatcatacat gatttttctc tgtatttcaa gtgtttttga
     3781 taatatttct catttttctt ttatcaattt caaaatccta cttcatatct gcttttgtat
     3841 ttttgtctct gtagtcttcc atttccgcat cgtataaaaa taatatggca caagtggaat
     3901 cagcgcagcg atccatgccg ccggatcggc gagacacact ccctgaaaag ttgtccttcc
     3961 ggcagccgcc ataacaatcg cacttcttgc tgcaagttca aaaacacctc ccagcatggg
     4021 aacaagtcca tatcccattc cctgaagcgt attccggtat aaaaaaatac ttcccaaaaa
     4081 cggataacac caaaacaccg tacgaaaata taatacagac acattgacaa cttctgtctc
     4141 agacggatcg ataaacagcc atgtcatata ttttccgcca aaacaaacgg ctgccattgc
     4201 gatcacactc cagactaata tcatgacctg tgtgcatctc acccccgact ttacccggtc
     4261 cattctccct gctccgcggt tctgtcctac ataagtagcg actgtagctc caaatgcaac
     4321 gaacaccgtc ataatcatat tttgcagttt ccctgcagcc gaaaaacctg ccatgtacgt
     4381 ttcaccatat acattaaccg ctccctgaac gataatcgtt ccgatcgccg taattgaaaa
     4441 ctgaagcccc atcggaatgc caagtgaaag aagacgcccg aaactttgaa aagaaatctt
     4501 tcgatctgat tttgtcaaat gcaaaatagg aaactttttc acaatataaa taaaacataa
     4561 caacgcagaa attgcctgtg caatgaccgt cgcataggca catccttcca cacccattcc
     4621 gaatacgacg ataaacacta catcaagagc tacattaatt accgcagata taataagaaa
     4681 atacagcgga gattttgaat cgcctatcgc acgcaaaaaa gccgccagtg aattatatgc
     4741 ggcagtcacg atcaaacctg cataaataat ccccatatac tgctttactt caggcatgat
     4801 ttccgcagaa taattcatca tccgtaaaat aggctcattc gcgatcaaaa gtcccgccgt
     4861 cataagaacg gcacatccgc ctgccagata aaccgacatc gccacgtaat gccgcatttt
     4921 atcatattgt tttgcaccga accactgtga aacaagaatt gcaaatccgc tgcttagtcc
     4981 gttaagccag cccgtcacaa aaaacatcag cgatccggta cttccgatag ccgccagagc
     5041 tttcactccg ataaattgtc ctgctacaat cgaatctgca atattgtaaa actgttgaaa
     5101 tatattcccc agaaaaacag gaatcataaa ctggataatc aattttgccg gggttcccac
     5161 tgtcatatca ttgactgcat cttttgtttt caccattttt cttttccccc ttctctcttc
     5221 tgtttgcatg tacttatcaa acgatcgcgc ctgatattgc ataaaatgca tccctaattc
     5281 attctgccga taaaattt
//
LOCUS       DBMDXF010000130         5279 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3863_length_5279_cov_1.687910, whole genome shotgun sequence.
ACCESSION   DBMDXF010000130 DBMDXF010000000
VERSION     DBMDXF010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3863_length_5279_cov_1.687910"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="ACZPD4_06095"
     CDS             <1..347
                     /locus_tag="ACZPD4_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HIR1378667.1"
                     /translation="ARNGDQSAIETLTLDDIDMYSQVSKRLANEDIFSIVDTYFMPYG
                     IECDLYSIMGEILAVRRRKNIVTNREVYQMKLNVNELQFDICVPVSETMGEPEIGRRF
                     KGTIWLQGYINF"
     gene            complement(381..1382)
                     /locus_tag="ACZPD4_06100"
     CDS             complement(381..1382)
                     /locus_tag="ACZPD4_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR1378668.1"
                     /translation="MEISNEDIEYDKELKIHLGSQDLKIKMSEGIQKGKIIEVTNYLT
                     KTHNVHAKIHMPLIINIDQPDNIEPYYTVYNYDRTFSLFACAAVLAAAIFCIGGGKGL
                     KSIAGLLYSMLLIIEFLLPAVFSGWPPIASSIICAILSTAVTLLLLNGQSGKTFSAIL
                     STMIGMFFALILFLITSAMIHVDGFSSADAEGLILIHEETGLQIKDVLFAGVVISSLG
                     AIMDVGMSVVSSLYEIYHHNPTLTAKDIFRSGIEIGKDMIGTMTNTLILAFTGSAFIT
                     LLVFLSYQVQFNQLINSNYLSIEIAQGLCGTFGIVLTIPAASAISAFMLTRKQKMIP"
     gene            complement(1546..3930)
                     /locus_tag="ACZPD4_06105"
     CDS             complement(1546..3930)
                     /locus_tag="ACZPD4_06105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purple acid phosphatase family protein"
                     /protein_id="HIR1378669.1"
                     /translation="MKKRIVQLQIGAAAIAAILGTGSVINAFPCSAALVTAYAKEQNT
                     EENVTVVNEKTTWKYLDNNTDPASGLSSLTAWTTPDFNDSAWKSAAGKFGAKRGALTS
                     FNGFTPTVLLQQYQDQENSKCTPTFFFRTTFNAQNIDQITSVTGTLFHDDAVAVYLNG
                     QLITSADMPDEKHENNLYYAGVSAGAPKEASVVLTKDQLKSILKEGSNVLSVELHQDR
                     ESSSDIYFEFQNLSLNYNENNTDGDNSGSNDEKVTQKSIFLTVGNDTSSQGITWYADT
                     ETAEEVQYAVKTGDTFPENYLTVPASSTAANEKGFYSNQAVLTGLLPDKEYVYRVKNG
                     DTISDIYSFTSGNNDGSYEFAFVGDPQIGAGSTDSDIEGWNETLKTISSKFNADFLLS
                     GGDQVNTASNETQYTGYINELFTSLPSATTIGNHDSGSAAYNQHFNLPNESADKGQTT
                     AGSDYWFVYENTLFINLNSNDRSTAEHKAFIEEAIAANPNVKWKTVVFHHSIFSTASH
                     VDDGDIITRRNELPQVFKDLDIDVVLMGHDHVYTRTYMMDGTTPDTSKGVQSSVTNPT
                     GILYLTANSASGSKYYDIKAPNAEYSAKMDQSYRRTVTDIKVTDTSYTMKTYYADDLS
                     LLDEFTIYKADNTALLNKINELKNMNLSESDYTKDSWKSFSDALTSAEELLKNSDATQ
                     DMLDASLLDLQTAYENLVKTDDQNGNDNNGNNNNGDNNNGNNNNGNNNNGNNNNGNNS
                     GNNNANNNNNSSNKANTSAPSAKTGDITTFAPIALLLAASLSVIAFITKRRRSA"
     gene            4446..4519
                     /locus_tag="ACZPD4_06110"
     tRNA            4446..4519
                     /locus_tag="ACZPD4_06110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4480..4482,aa:Arg,seq:cct)
     gene            complement(4704..>5279)
                     /locus_tag="ACZPD4_06115"
     CDS             complement(4704..>5279)
                     /locus_tag="ACZPD4_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378670.1"
                     /translation="TTRVVDKYIQTLQKTPSVSRKNRKARTEFVTNSTIEKIIKLLRC
                     AFKQAVRWELIEKNPFDNVVLPKTEYKKRDIWDAETIRLALDQCTDSKLYIAMNLAFA
                     CSLRMGEILGLTWKNVHIEDENIATDNAYIYIEAELARASKQAIETLGEKDIYHIFTP
                     LMPNTSTRIILKKPKTDSSIRKVWLPKTVAY"
BASE COUNT         1572 a          950 c         1077 g         1680 t
ORIGIN      
        1 cagcaagaaa cggagatcag agcgcgatag aaacactgac attagacgat atagatatgt
       61 attctcaagt ttcaaaaaga cttgcaaatg aggatatatt ttctatagtg gatacgtatt
      121 ttatgccgta tggaatagag tgcgatcttt attctatcat gggagagata ctggcagtcc
      181 gcagacgaaa aaacatcgtc acgaacaggg aagtttatca gatgaagctt aatgtaaatg
      241 aacttcaatt tgatatatgt gttcccgtat ccgagacgat gggagaacct gagatcggaa
      301 ggcgctttaa aggaacgata tggctgcagg gatatattaa tttttaatat gaataatcgc
      361 cggaagagcc gaaaggcaca ctacgggatc atcttttgtt ttcgagtcag cataaatgca
      421 gagattgcag aagcggctgg aattgtcaga acaattccga atgttccgca tagtccctga
      481 gcaatttcga tgctgagata attagaattg ataagttggt taaattgtac ttgatatgag
      541 aggaacacaa gtaatgtgat gaatgcactg cctgtaaatg caaggatcag tgtattcgtc
      601 attgttccga tcatatcttt tcctatttct ataccggatc tgaaaatatc ttttgcagta
      661 agagtcggat tatggtgata aatttcgtaa agtgatgaaa cgacagacat tccgacatcc
      721 atgatcgcac cgagagaaga aattacaact cccgcaaaaa gaacgtcttt aatctgtaga
      781 ccggtttctt catgtattaa aatcaaacct tcggcatctg ccgaactgaa tccgtccaca
      841 tggatcatgg cagatgtgat aaggaaaaga ataagcgcaa aaaacattcc aatcattgta
      901 gataaaatcg cggaaaatgt tttcccgctt tgtccgttta acagaagaag agtaactgcc
      961 gtggacagta tggcacatat gatactggat gcgatcggcg gccatcctga gaatacggca
     1021 ggcaggagaa attcaatgat aagaagcata gaataaagaa gccctgcgat tgattttaat
     1081 ccctttcctc cgccgataca aaagatcgcg gctgcaagaa cggcggcaca tgcaaaaaga
     1141 gaaaatgtgc ggtcataatt gtatacagta taatatggct caatattatc aggctgatcg
     1201 atattgataa taagaggcat atggattttc gcatgaacat tatgtgtttt cgttagataa
     1261 tttgttactt ctataatttt acctttttga attccttcgg acatttttat ttttaaatcc
     1321 tgactgccga ggtgaatttt aagttcttta tcatattcaa tgtcctcatt agaaatctcc
     1381 actacgacac ctttgtcgta gtggagtgta tctgtgctgt atcgagaata gcttttaaac
     1441 ccgaaattct gaagaataag tatagtgagg acactgagga aaataagtat cagtacaata
     1501 tagttttgct ttatttttct caacatcatt ttatcctatc tgatcttaag cagatctcct
     1561 tctttttgta ataaatgcaa taacggaaag tgatgcggca agcagcaaag ctatcggagc
     1621 aaaagtggtg atatcgccgg tttttgcgga cggagcagaa gtatttgctt tattactgct
     1681 gttgttattg ttgttggcat tattattgcc gctgttattg ccattgttgt tattgccgtt
     1741 gttgttattg ccgttattgt tattgccgtt attgttatcg ccgttattgt tattaccatt
     1801 gttatcatta ccgttttgat catcggtttt gacaagattc tcgtaagctg tctgaagatc
     1861 taaaagcgat gcatccagca tatcttgtgt ggcatcagaa ttttttaaca gttcttctgc
     1921 tgatgtaaga gcatcggaaa aggacttcca actgtctttt gtataatcag actccgataa
     1981 attcatattt ttaagttcat ttatcttatt gagaagagct gtattatccg ctttgtagat
     2041 tgtaaattca tctaataaag atagatcatc ggcgtaataa gttttcattg tgtaagaagt
     2101 atctgtcact ttaatatctg ttaccgttct tctgtaagat tgatccatct tagcggaata
     2161 ttcggcattc ggagctttga tatcatagta tttactaccg gaagcagagt ttgcggtaag
     2221 ataaagaatt cctgtcggat ttgtaacgct gctctgaact cctttacttg tgtcaggagt
     2281 tgttccgtcc atcatataag ttcttgtgta aacatgatca tgtcccatta atacaacatc
     2341 aatatcaaga tctttgaaaa cttgaggaag ttcattacgt cttgtaatga tatcgccgtc
     2401 gtcaacatga cttgcagttg agaaaataga atggtgaaaa acaactgttt tccattttac
     2461 attcggattt gcagcgattg cttcttcgat aaatgcttta tgttctgcgg tggatctgtc
     2521 attggagttt aaattaatga aaagtgtatt ttcgtaaaca aaccagtagt cgcttccggc
     2581 agttgtctgt cctttatcag cgctttcgtt tggcaggtta aaatgctgat tatatgcggc
     2641 agaacctgag tcgtggtttc cgattgtcgt agcgctcgga agggaagtaa acaactcatt
     2701 tatgtatcct gtatactgtg tttcatttga ggccgtgttt acctgatctc cgcctgaaag
     2761 aagaaagtcg gcgttaaatt ttgaagaaat agttttcaaa gtttcattcc atccttcaat
     2821 atcgctgtct gtgctgccgg cgccgatctg aggatctcct acgaaggcga attcataaga
     2881 accgtcgttg tttccggaag taaaactata aatatcactg attgtatcgc cattttttac
     2941 acgatacaca tattctttat caggaagaag tcctgtaaga accgcttgat tagaatagaa
     3001 tcctttttcg tttgcagctg ttgaagaagc gggtacagtt aagtaatttt cagggaatgt
     3061 atcgcctgtt tttaccgcat actgaacttc ttctgccgtt tctgtgtcgg cgtaccacgt
     3121 aattccctga cttgacgtat cgtttcctac agttagaaaa atagatttct gcgttacttt
     3181 ttcatcattg cttccgctgt tatcgccgtc tgtattattt tcattatagt ttaaagacaa
     3241 attctgaaat tcaaagtaaa tatcagaact ggattcacgg tcttgatgaa gttctacaga
     3301 aagaacgttt gatccctctt ttaagatgct tttcagttga tcttttgtaa gaacaactga
     3361 agcttcttta ggagctcctg cgcttactcc tgcataataa agattatttt catgcttttc
     3421 atccggcatg tcggctgaag tgatcaactg tccgtttaag taaacggcaa cagcgtcgtc
     3481 atggaaaaga gttcctgtga cagaagtgat ctgatcaata ttttgtgcat taaaagttgt
     3541 acggaagaaa aatgtcggag tacatttgct gttttcttga tcttgatatt gctgtaaaag
     3601 aactgtcgga gtaaatccgt taaaagatgt aagagcgccc cttttcgcac cgaattttcc
     3661 ggcggcagac ttccatgcag agtcattaaa atcaggagta gtccaagcag tgagcgagga
     3721 cagcccggat gccggatctg tgttgttgtc aagatatttc catgttgtct tttcattgac
     3781 tacggttaca ttttcctcgg tattctgctc ttttgcgtaa gcagtgacca aagcggcgga
     3841 acacggaaat gcatttataa cgcttcccgt tccgagaatt gctgcaatcg ccgcagcgcc
     3901 gatctgtaat tgaacgattc gttttttcat aataacctcc tgtaaaagtt cctgttttat
     3961 tcgtatgatg ccgtgacggt aaaagacggt cacatgccga tagttatcgt agcatttgaa
     4021 aaaaacacat acaatagacc ttacagtcat ttaaaataaa tgacaaagat ttaaaataga
     4081 gagaaaaact taacaaaaat aaaaaccaga atttaacgta aaactaacaa aaataaaaat
     4141 catggaaaaa atagtggaag caaaatgatt tcagaaaaat acgaaaaacg gttgtagcag
     4201 acagatgaaa ctgttataat agatgaggta tttacaaata ttaaaaaagt aaaataattg
     4261 taattaacag cttcaaaaga gctgataatt aaaataaagg ttgccataac aaaaatcctg
     4321 aaaacagaat gttttcaagg aaaagcggca tttgtctcgt tagttaaatg gatataacag
     4381 ggtcctccta agcggtagat gattcgagtc taaattttgc atacaactta atatttttga
     4441 tagatgtctc agtagctcag ttggatagag cacacgcctc ctaagcgtgg ggtcggaggt
     4501 tcaaatcctc tctgggacgc tggaaacaac gctgtaagcc tttgttttca agggtttaca
     4561 gctttttttg tttagctgta ctctttagac aaaaccattt ttcttttatt ggaggttaat
     4621 gtatgtttat gagttggaac ttatattttg ctaattggaa acgaacagaa acgatgtcct
     4681 tgctaataaa aatagcttcc ttgctaatag gcaactgtct taggcagcca gaccttacgg
     4741 atgctggaat ctgtctttgg ttttttcagg attatccggg tgctggtatt cggcattaac
     4801 ggagtaaaga tatggtaaat gtctttttct cctaacgtct caatcgcctg tttggacgct
     4861 cgtgccagtt ctgcttctat gtagatataa gcattatcag ttgctatgtt ttcatcctca
     4921 atatgaacat ttttccaggt tagtccaaga atctccccca tacgcaaaga acacgcaaaa
     4981 gcaaggttca tggcaatata cagtttactg tcggtacact gatccaaagc aagacgaatc
     5041 gtttcagcat cccaaatatc gcgcttttta tattcagtct tgggaaggac tacattgtca
     5101 aaaggatttt tttcaatcaa ttcccaacga acagcttgct taaaagcaca ccgtagaagt
     5161 ttgatgattt tctcaatcgt tgagttcgtg acaaattctg tccgtgcttt cctattcttc
     5221 cgagaaacag aaggcgtctt ttgaagtgtt tgaatatact tatccaccac gcgggtagt
//
LOCUS       DBMDXF010000131         5182 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3950_length_5182_cov_1.922456, whole genome shotgun sequence.
ACCESSION   DBMDXF010000131 DBMDXF010000000
VERSION     DBMDXF010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3950_length_5182_cov_1.922456"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..2616)
                     /locus_tag="ACZPD4_06120"
     CDS             complement(181..2616)
                     /locus_tag="ACZPD4_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1378671.1"
                     /translation="MARRKKLQVEEKKERRLPTTRYNPEYKKGLTSQQVQEHRLHGWT
                     NKAVEPPSKTTKEIVHENVFTYFNLIFVVLAVLLCLVGSFRDLTFLPVIIANTLIGII
                     QEIRAKQVLDKLTMLNAPRASVVRDGKRTVINAEDLVVDDIVIFKAGDQVCADAEVSA
                     GEVQVNESLLTGEADEITKRKGDKLMSGSFIVSGQCHARLDKVGEDSYISKLTLQAKA
                     MQSKEQSEMIRSLDKLVKCVGVAIIPIGIVLFSQAFFIQHDGFRESVTSMIAAVIGMI
                     PEGLYLLASVALAVSSIRLAQKKVLLHDMKCIETLARVDVLCVDKTGTITENTMKVQK
                     LIKTDEYDEKEKGGLSLLVGDFAAAMTNDNITMAALKEYFTKASGKKVLSKTGFSSAT
                     KYSSVTFEDGAYVLGAPEFVLKEKYDDYAEEITEYASTGSRVLAFGIYDGEVDGKPLT
                     HGILPFGFVLLANPIREAAKETFEYFAEQGVEVKVISGDNPVTVSNVAKQAGIKNADR
                     YVDASEFEDEQSMRKALLNNTVFGRVTPSQKRKFVRILKEAGHTVAMTGDGVNDVLAL
                     KDADCSIAMASGSDAAAQASQLVLLESDFSCMPEVVLEGRRVVNNIQRSASLFLVKNI
                     FSFLLSVASVVFMFTYPLEPSQVSLISMFTIGVPAFFLALEPNKNMIKGHFLTNVLLK
                     ALPAALTDALAVAALVIFGRTFDVSSTDISTAATMLLAIVGFMILYKISAPMNKIRFS
                     IVSGCIAGLLFCSIFLKDLFAITSMTKECIMLFVVFAIATEPVLRYLTTLVEKVKYYY
                     LKLRGKNLSSDDT"
     gene            complement(2786..4723)
                     /gene="ftsH"
                     /locus_tag="ACZPD4_06125"
     CDS             complement(2786..4723)
                     /gene="ftsH"
                     /locus_tag="ACZPD4_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1378672.1"
                     /translation="MKQVQKPRKPLIVYYLIAIAVLILFNSFVFPAIVGGNVEEVDYG
                     TFLTMVENKEVSKVQLEGESIYFTDKSEDSRQYETTRFDDPDLVNRLEKAGCKFGRVA
                     DRQMSPFLSMLLSIVIPMIIFILLGQFLSRQLMKKMGGAAGGNQFMQFGKSNAKVYVQ
                     STTGITFNDVAGEDEAKELLTEIVDFLHNPQKYQEIGAVCPKGALLVGPPGTGKTLLA
                     KAVAGEAGVPFFSISGSEFVEMFVGMGASKVRDLFKQANEKAPCIVFIDEIDTIGKKR
                     DSHGMAGNDEREQTLNQLLTEMDGFDASKGVVILAATNRPDTLDPALLRPGRFDRRIP
                     VELPDLKGREEILKVHARKIKLGDNVDFNAIARAASGASGAELANMVNEAALRAVREN
                     RKFVVQSDLEESIEVVIAGYQKKNRVLSTKEKLIVAYHEIGHALVAALQTHSAPVTKI
                     TIIPRTSGALGYTMQVEEEERNLMTEEELKNKIATLTGGRCAEELVFGSVTTGASNDI
                     EQATKLSRAMITRYGMSSRFGMVALETQSNPYLGGDSSLSCSPETAATIDDMVVDMVK
                     QSYEKARKLLKDNQGKLHELAKYLYEKETITGDEFMRILSQKELSSDGETASGAEFAA
                     DGRQILEEEKASDKEVHISEE"
     gene            complement(4787..>5182)
                     /locus_tag="ACZPD4_06130"
     CDS             complement(4787..>5182)
                     /locus_tag="ACZPD4_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HIR1378673.1"
                     /translation="MLKIGTEGLSIGITTMIAFLIGYKGGNALLGSTMAFATLCSARL
                     FHGFNCKSDRPVVFTSKLLNNIYLIGAFVVGLALVTMVVMIPGLHSIFKVQTLNLTQL
                     LIVYGLAAVNIPIIQILKAIRLALKKKEK"
BASE COUNT         1383 a         1307 c          876 g         1616 t
ORIGIN      
        1 ttcaataaga tttttactaa cctgccagtc agtagtctta ttgtactctg aggtattttc
       61 aattttcaac atccacattt tttattatac aggaagctcc ttaatcggca tcattcattt
      121 ttaagctgct ttgctcctaa tatccattca ttttccgtat ctttcttatt cttgaaaata
      181 ttatgtatca tctgaagaca gatttttgcc tcgcagcttc agatagtaat atttcacttt
      241 ttcgacaagt gttgtcaaat aacgaagcac cggttctgta gcaatcgcaa atactacaaa
      301 gagcataata cattcttttg tcatacttgt aatagcgaac agatctttta ggaagatgct
      361 gcagaacaat aatcctgcaa tacatccgct tacaatacta aaccgtattt tattcatcgg
      421 cgcgctgatc ttatataaga tcataaatcc tacgatcgcc aaaagcatcg ttgccgccgt
      481 tgatatatcc gtagagctta cgtcaaatgt tcttccaaat atgacaagag ccgcaactgc
      541 aagagcatct gtcaacgcag caggaagcgc ttttaatagc acatttgtaa ggaaatgtcc
      601 tttgatcata tttttattcg gttccagagc aaggaagaat gcaggaacac cgattgtaaa
      661 catactgatc aatgatactt gagacggctc caacggataa gtaaacataa acacgacaga
      721 cgcaacgctt agcaaaaatg agaaaatatt cttcacaaga aataaacttg ccgatctctg
      781 aatattattt acaacccgcc ttccttccaa cacaacttcc ggcatacatg aaaaatctga
      841 ctccagcaaa acaagctgag atgcctgtgc cgcagcgtcg cttcccgatg ccatggcaat
      901 actgcagtcg gcatctttca aggcaagcac atcattaacc ccgtctcccg tcattgctac
      961 cgtatgtcct gcctctttta aaatacggac aaacttccgt ttctgactcg gcgtaacccg
     1021 accgaaaacc gtattattca gaagcgcttt cctcatggac tgttcatctt caaactctga
     1081 cgcatctaca tatctgtctg catttttaat tccggcctgc tttgcaacat tggaaacagt
     1141 aaccggatta tcccctgaga taacctttac ttctacgcct tgttctgcga aatactcaaa
     1201 cgtctctttt gcagcttctc gtataggatt ggcaagtaaa acaaagccaa acggaagaat
     1261 gccatgtgtc agaggttttc cgtcaacctc tccgtcataa attccaaaag caagaactct
     1321 ggatcctgta gatgcatatt ctgtgatctc ttccgcataa tcgtcatact tttcttttaa
     1381 aacaaattcc ggcgctccga gcacataagc tccgtcttca aatgtaacgc tgctgtattt
     1441 tgttgcggaa gaaaatcctg tttttgacaa aaccttcttt cctgatgctt ttgtaaaata
     1501 ttcttttagg gcagccatcg taatattatc atttgtcata gctgccgcaa aatctccgac
     1561 aagcaaagat aacccgccct tttctttttc atcgtactca tccgtcttga tcagcttttg
     1621 gactttcatt gtattttccg taatcgttcc tgtcttatcc acacaaagca cgtctacacg
     1681 ggcaagcgtt tcaatacatt tcatatcatg caaaagcact tttttctgtg caagacgaat
     1741 tgaactcacc gcaagcgcca cacttgcaag aagatacaat ccttccggaa tcattccgat
     1801 caccgccgcg atcatagatg tcacgctttc tctaaatcca tcatgttgaa tgaaaaatgc
     1861 ctgtgaaaaa agaacgattc cgatcgggat aatagctact cctacacatt taacaagctt
     1921 atcaagcgaa cgaatcattt ccgactgttc ttttgactgc atcgcttttg cctgcaatgt
     1981 caatttcgat atatatgaat cctctcctac tttatcaagc cgcgcgtgac attgtccgga
     2041 aacaataaag ctgcctgaca tcagtttatc gcctttacgt tttgtaatct catcggcttc
     2101 acctgtcaaa agcgattcat tcacctgaac ttctccggca gatacttccg catcggcaca
     2161 cacctgatcg ccggctttaa aaattacgat atcatctacg acaagatctt cggcattgat
     2221 caccgttctt tttccgtctc ggacaacaga ggcgcggggc gcattcaaca tagtcagctt
     2281 atccagtact tgttttgctc taatctcctg aataattcca atcaatgtat ttgcaataat
     2341 aaccggaaga aatgtcaaat ctctgaaaga tcccacaaga cagagcaata ctgcaagcac
     2401 gacaaagatt aagttgaaat aagtaaatac attttcatga acaatctctt tcgtcgtctt
     2461 tgacggaggt tcaaccgcct tgttcgtcca accgtgcagc cggtgttcct gaacttgctg
     2521 acttgtcagt ccctttttat attcgggatt atatcttgtt gtcgggagcc gtcgttcctt
     2581 tttttcttct acctgcaatt tttttcttct tgccatacag cttttcacct ctgtctcttc
     2641 ttttgtgtct aacttcttat tgctgttctt ttttattttc tattcatacc gttgtacaag
     2701 ggtagcgtcg atgtacagac cgtaaacggt tctttctgta cagcgacgct atcgcctgca
     2761 attttttaat tttaacagat aatctttatt cctcagaaat atgcacttct ttgtctgatg
     2821 ctttttcttc ctctaaaatc tgtctgccat ctgccgcaaa ctctgcgcct gatgcagtct
     2881 ctccatctga agaaagttct ttctgtgaca agatccgcat aaattcgtcc cctgtgattg
     2941 tttctttctc atacaaatat ttggcaagtt catgcaattt cccctgatta tctttcaaca
     3001 gctttcgcgc cttctcataa gactgtttta ccatatctac aaccatatcg tcaatagttg
     3061 ccgctgtctc aggagaacaa cttaaactgc tgtcgcctcc gaggtacgga tttgactgcg
     3121 tctcaagagc aaccattcca aatcggctgc tcataccata tctggtgatc attgccctgg
     3181 aaagcttcgt cgcctgttca atatcatttg acgctcctgt tgtcactgag ccgaatacaa
     3241 gctcctccgc gcatcttcct cctgtcaatg tcgcaatttt atttttaagt tcttcttcag
     3301 tcatcagatt acgttcctct tcttctacct gcatcgtata tccgagcgca cccgaagttc
     3361 tgggaatgat cgtaattttt gtcaccggag ccgaatgtgt ctgtaatgcc gccaccaaag
     3421 catgaccgat ctcatgatat gcgacgatca gtttctcttt cgtcgaaaga acacggtttt
     3481 tcttttggta tcccgcgatc acaacttcaa tactttcctc tagatccgac tgcacaacaa
     3541 attttctgtt ttcacggaca gcccgaagag ccgcctcatt caccatattt gccaactccg
     3601 ctcctgatgc tcccgaagct gctcttgcaa tcgcattaaa atccacatta tccccgagtt
     3661 ttatttttcg tgcatgcact ttcagtattt cttcacgccc tttaagatcg ggaagttcta
     3721 cagggattct ccggtcaaaa cgtcccgggc gcaaaagcgc cggatccaat gtgtccggtc
     3781 ggtttgttgc cgcaagtatt acaacacctt tcgacgcatc aaaaccgtcc atttctgtaa
     3841 ggagctgatt caatgtttgc tcccgttcat catttcctgc cattccgtgg ctgtcacgtt
     3901 tcttgccgat cgtatcgatc tcatcgataa atacaataca aggggctttc tcatttgcct
     3961 gtttaaacag atcacgcact tttgaagctc ccatgccgac aaacatttct acaaattccg
     4021 aacccgaaat agagaaaaac ggaactcccg cttcccctgc aacggctttt gcaagaagcg
     4081 tctttcctgt tcccggaggc cccacaagga gggcgccttt cggacatacg gcgccgattt
     4141 cctgatattt ctgaggatta tgaaggaaat caacaatctc tgtcaaaagt tcttttgctt
     4201 catcttcacc tgccacatcg ttaaacgtaa tacccgtcgt agactgcaca tatacttttg
     4261 cattgctctt tccaaactgc ataaactggt ttccgcctgc cgctccgccc atctttttca
     4321 tgagttgacg tgacaaaaac tgtccgagga gaatgaaaat gatcatcgga atcacgattg
     4381 ataagagcat acttaaaaac ggactcatct gtcgatccgc aactcttccg aacttacatc
     4441 ctgctttttc caaacggttt acaagatccg ggtcatcaaa cctcgtagtt tcatactgtc
     4501 tggaatcttc acttttatct gtaaaataaa tgctttcgcc ttcgagctgt actttcgata
     4561 cttctttgtt ttctaccatc gtaaggaacg tgccgtaatc aacttcctca acatttcccc
     4621 ctacaatggc agggaataca aaggagttaa aaaggatcag caccgcaatc gctatcagat
     4681 aatatacgat caatggcttt cttggttttt gtacttgttt cataaaagtc ctcctttctg
     4741 tatccctctg cttctcttct gttaaaccaa tcttttaaag agagctttat ttttcttttt
     4801 tcttcagcgc cagacggatt gctttcaaaa tctggatgat cggaatattc actgccgcaa
     4861 ggccgtatac gatcaaaagc tgcgtcaaat tcaacgtctg tactttaaaa atactgtgaa
     4921 gtcccggaat cataacaacc attgtcacta atgccagacc tacaacaaat gcacctatca
     4981 aataaatatt atttaaaagc ttgcttgtaa atacaacagg tctgtctgat ttgcagttaa
     5041 atccatggaa caatcttgca gagcacagtg tcgcaaatgc cattgtactt ccaagcaatg
     5101 cattaccgcc tttatatccg atcaaaaatg cgatcattgt tgtgattccg attgacagtc
     5161 cttctgttcc gattttcagc at
//
LOCUS       DBMDXF010000132         5175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3956_length_5175_cov_1.778084, whole genome shotgun sequence.
ACCESSION   DBMDXF010000132 DBMDXF010000000
VERSION     DBMDXF010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_5175_cov_1.778084"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            97..468
                     /locus_tag="ACZPD4_06135"
     CDS             97..468
                     /locus_tag="ACZPD4_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1378674.1"
                     /translation="MNFTFAHNNLNVYDLDASLRFYKEALGLTVTRTKEADDGSFKLV
                     YLGDSTTPHKLELTWLRDMDRPYNLGDNEIHLAFETDDFDAAHEKHKEMECICFENPG
                     MGIYFISDPDGYWLEIVPKNL"
     gene            582..950
                     /locus_tag="ACZPD4_06140"
     CDS             582..950
                     /locus_tag="ACZPD4_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1378675.1"
                     /translation="MEHYTLPHRHNQSMSQLMSHLPNIEEFQTTADIFKQLSDPTRIR
                     IFWILCHCEECVINIASMMEMSSPAVAHHLRLLRSSGLIESRRDGKETYYRAVDSKKT
                     QTLHHMIEKIMDISCINKDV"
     gene            complement(970..2856)
                     /locus_tag="ACZPD4_06145"
     CDS             complement(970..2856)
                     /locus_tag="ACZPD4_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1378676.1"
                     /translation="MSKKQKKMLTRIMAAAFGVILLNFVPVTGILRFTLYLAVYLVIG
                     YDILKKAGRGILNRRMFDENFLMAVATIGAFALAVYSKSGDYNEAIAVMLFYQIGELF
                     QSYAVGKSRRNISALMDIRPDYANVECDGKLEKRDPDEVGIGSIIVVQPGEKVPLDGI
                     VVEGSSSLNTSALTGESLPRDVRQGEEVISGCINMTGVLKVRTTKQFEESTVSKILEL
                     VENSGSRKSKSEHFISKFAKVYTPAVCYGALALALLPPVIEMVFLGQAAQWGVWIYRA
                     LTFLVISCPCALVISIPLSFFAGIGGASKAGVLIKGSNYMETLSQTKYVMFDKTGTLT
                     KGAFEVSAVHHNELEEEKLLEYAALAECASSHPISKSLQKAYGKEIDRNRVSDIREIS
                     GHGITAVVDGHEVAAGNDKLMKKLGLKYHECHTAGTIIHMAIDKKYAGHIVISDVVKE
                     HSKEAVAELKKAGIKKTVMLTGDSRKAAEQTAKTLGIDQVYSELLPGDKVQKVEELLL
                     EKEGKEKLVFVGDGINDAPVLTRADIGIAMGAMGSDAAIEAADVVLMDDDPLKISKAI
                     KISQKCLRIVYQNIIFALAIKFACLGLGALGIANMWFAIFADVGVMIIAVLNAVRALR
                     VQNL"
     gene            complement(3001..3219)
                     /locus_tag="ACZPD4_06150"
     CDS             complement(3001..3219)
                     /locus_tag="ACZPD4_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1378677.1"
                     /translation="MKKTYKIEVDCANCANLMEDAAKKTGGVEDAVVNFMTQKMTVQF
                     EDGREPKSVMKDVLKACKKIEPDCEIEF"
     gene            complement(3445..4569)
                     /locus_tag="ACZPD4_06155"
     CDS             complement(3445..4569)
                     /locus_tag="ACZPD4_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR1378678.1"
                     /translation="MMLLCGEIDNKMRKGGSMRLKKVHSTFLTAALACSLAVTPVFAN
                     PAEDAENLKNQKTEAESELSNLQTQFDTLIQKANELELKLIKTGEAIIQAQADLEAAE
                     EKKEEQYEAMKKRIKFMYESGTGSATVEKVMTSGDMSSMLSQAEYSQKMHEYDREQLK
                     EYTNTVTEIENLQKTLEEEQKNLEATEKEYEAEQATLTETINSKKDEITNLDGMIQEA
                     ARKAEEERKAEEERRRREEEAKNNVTPEQPVTPSNPNNGSSGGSGAAPYDPVTGNVIV
                     DRAYGCIGLPYVWGATGPNSFDCSGLVGYCLTGGYGRVGTTYTFMGWPRVSDPQPGDV
                     CTNWDHCGIYIGNGMMIHAPQPGENVKIGPVQAGMVYVRR"
     gene            complement(4806..5135)
                     /locus_tag="ACZPD4_06160"
     CDS             complement(4806..5135)
                     /locus_tag="ACZPD4_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1378679.1"
                     /translation="MKAYADMISESKDAVCIFTEEFGGDSMRMLMNYVLDAGHCICAV
                     FFGNEKDGYRYVLGSRQQDVREMAKEFNKRFDGRGGGKPEMVQGTVRGEKEAIERWLM
                     KYEFVCI"
BASE COUNT         1342 a         1335 c          856 g         1642 t
ORIGIN      
        1 ccagacaagt atcctgctga ttccggcagg cggaatggcg ctgctgatcg gatatatttt
       61 atggaaaaaa gacaagcttt gataaggagg agcattatga actttacttt tgcccacaac
      121 aacttaaatg tctacgatct ggatgcatct ttgcgatttt ataaagaagc tctcggtctt
      181 actgtcacac gtacaaaaga ggctgacgac ggcagcttca aacttgttta tctcggtgac
      241 tcgacaactc ctcacaaact cgaactgaca tggcttcggg atatggaccg cccttacaat
      301 ttgggagata acgagatcca tcttgcattt gaaaccgatg attttgatgc ggcacacgaa
      361 aaacacaaag agatggaatg catctgtttt gagaatccgg ggatggggat ttatttcatt
      421 tccgatcctg acggatattg gctggagatc gtacctaaaa atttataata ttaaaaaggg
      481 cagtttttta tgatcgctga aaaacccggc gcacagagac gccgggtttt ccgatttcca
      541 aaaaatatat tatacaaact atgtccgaaa ggagttttct tatggaacat tatacattgc
      601 cccatcggca caatcagtca atgtctcagt taatgagtca tttaccgaat attgaagagt
      661 ttcagacgac cgcagatata tttaaacaac taagcgatcc tacccgcatc cgcatcttct
      721 ggatcttgtg ccactgcgaa gaatgtgtta tcaacatcgc ttctatgatg gaaatgagca
      781 gtcctgccgt cgcgcatcat ttacgtctgc ttcgttcaag cggtctgatc gaatcccgca
      841 gggacggaaa agaaacatat taccgtgccg tcgactcgaa aaaaacacaa acgcttcatc
      901 atatgatcga aaagatcatg gatatttcct gcataaacaa agacgtataa atcaaaattt
      961 aactcagatc tatagatttt gaactctaag cgccctgact gcatttaaga ccgcaatgat
     1021 cataacccct acgtcggcaa agatcgcaaa ccacatattt gcaattccaa gagcgccaag
     1081 tccaagacag gcaaatttaa ttgccaaagc aaatataatg ttttgatata cgattctcag
     1141 acatttttga gagattttaa tcgctttgga aatctttaac ggatcatcgt ccatcaacac
     1201 tacatctgcc gcttcgattg ccgcgtccga tcccattgcc cccattgcaa ttccgatatc
     1261 cgcccgagtc agtaccggtg cgtcattaat gccgtcacct acgaatacaa gcttttcttt
     1321 tccttctttt tccaagagca gttcttctac tttttgtact ttatctccgg ggagcagttc
     1381 actgtacact tgatcgattc ccaaagtttt tgccgtctgc tcggcagctt tccgcgaatc
     1441 cccggtaagc atgactgttt ttttgatccc tgcttttttc aattccgcaa ccgcttcttt
     1501 ggaatgttct ttcacaacat cggaaatcac gatatgccct gcatactttt tatcaatcgc
     1561 catatggatg atcgtacctg ccgtatggca ttcatgatac ttcaaaccga gttttttcat
     1621 cagcttgtcg ttacctgccg caacttcatg accgtccacg acggctgtaa tcccatgacc
     1681 gctgatttcc cggatatccg aaaccctgtt tctgtcaatt tcctttccgt atgctttttg
     1741 cagacttttg ctgatcggat gtgatgacgc acattctgca agcgccgcat attctaataa
     1801 tttttcttct tccagttcat tgtgatgaac cgcgcttact tcaaaagcgc cttttgttaa
     1861 agttcctgtt ttgtcaaaca taacatattt tgtctgagac aatgtttcca tataattaga
     1921 gcctttgatc aacactcctg ctttgcttgc cccgccgatt cccgcaaaaa agctaagagg
     1981 aatactgatc acaagtgcac agggacaact aatcacaagg aatgtaagag ctctgtaaat
     2041 ccacacgccc cactgagccg cctgtccgag aaagaccatc tctattaccg gaggcaagag
     2101 tgccagcgcc aatgccccgt agcaaactgc cggagtatac actttggcga atttggagat
     2161 aaaatgttcg gattttgatt tcctggaacc ggaattttcc acaagttcca aaatcttaga
     2221 caccgttgat tcttcaaact gtttcgtagt ccgaactttc agtactcccg tcatattaat
     2281 acagccgctg atcacctctt ctccctgacg cacatcacgc ggaaggcttt ctcctgtcaa
     2341 agcgcttgta tttaaactgg agcttccttc gacgacaatc ccatcaagcg gaactttttc
     2401 acccggctgt actacgatga tacttcctat tccgacttca tcaggatctc ttttttccag
     2461 tttcccgtca cattctacat ttgcataatc cggtcggata tccattaatg cactgatatt
     2521 tctgcggctt ttcccgactg cataactttg gaacaactct ccgatctgat aaaacaacat
     2581 taccgcgatt gcctcgttat aatcgccgct ttttgaatag acggcaagag caaaagctcc
     2641 gattgttgcg actgccatca gaaaattctc gtcaaacatg cgccggttca aaatacctct
     2701 gcctgccttt ttcaaaatat cgtatcctat gactaaatat actgctaaat aaagtgtgaa
     2761 ccgcaaaatt cccgttacag gtacaaaatt caatagaata acaccaaatg ccgctgccat
     2821 gatcctcgtc aacatttttt tctgtttttt actcattaca ccttgcatcc tcctcgctat
     2881 ttaatataca catatgttca ttcgttcata tgttgtatat aaatctgcgc cctttgccat
     2941 cagcatatcg ggcgctcctt tttcttttta gccactttca ttcaatcttt gagaagtttc
     3001 ttaaaattca atttcacaat ccggctctat ttttttgcag gctttcagca catctttcat
     3061 aaccgacttc ggctctcgcc catcctcgaa ctgtacagtc attttctgtg tcatgaaatt
     3121 caccaccgca tcttctactc cgccggtctt tttcgccgca tcttccatca gattcgcaca
     3181 gtttgcacaa tctacttcaa ttttataagt ctttttcata ttaaatacct cttttctttt
     3241 aaaatctttt tatttcaaca attaagcatt tatttaattg ttatgctttt actatacacc
     3301 tatctttttt ctctgtcaat ccttaaaaat gaaatctatt aaaaaaagca ggagctgtaa
     3361 aattcacaac tcctgcgatc gacctatgta ttattcactt gattttatac atcttatcat
     3421 aacttaccga ctgactttgt acgcctagcg acgaacataa accattcctg cctgtaccgg
     3481 tcctatctta acattctctc ccggctgagg cgcatggatc atcataccgt ttccaatgta
     3541 aataccgcaa tgatcccagt tcgtacatac atcgcccggc tgaggatcac tgacgcgagg
     3601 ccatcccata aatgtatacg ttgttcctac acgtccataa ccgcctgtca gacaatatcc
     3661 tacaagtccc gagcagtcaa aagagttcgg tcctgtcgct ccccacacat aagggagtcc
     3721 gatacatcca tatgcacggt ctacaataac atttcccgtt accggatcgt acggcgccgc
     3781 tcctgaaccg cctgaacttc cgttattcgg attggaaggt gtcaccggct gttccggagt
     3841 cacattgttc tttgcttcct cttcccgtct gcggcgttct tcctctgctt ttcgttcctc
     3901 ttcggctttt cttgcagctt cctggatcat gccgtccaaa ttggtgatct catctttttt
     3961 agaattgatc gtctcggtca gagtcgcctg ctcagcctcg tactcttttt ccgttgcttc
     4021 cagattcttt tgctcttctt caagtgtctt ctgcaaattt tcaatctctg ttactgtatt
     4081 tgtatattct ttcagttgtt cacgatcata ttcatgcatc ttctgcgaat actctgcctg
     4141 actgagcatg ctcgacatat cgccggatgt cataactttc tcgactgtag cgctgcctgt
     4201 tccggattcg tacataaact taatccgctt tttcatcgcc tcatactgtt cttctttttt
     4261 ttcttccgca gcttccagat ctgcctgcgc ctgaataatc gcctcacctg tcttaatcag
     4321 cttaagttcc agttcattcg ccttttggat caatgtatca aactgcgtct gaagattaga
     4381 aagctcgctt tccgcttctg ttttctgatt tttcaaattc tccgcatctt ctgccggatt
     4441 tgcaaacacc ggcgtcactg ctaatgagca agccaaagcc gccgtcagaa atgtgctatg
     4501 tacctttttt aatctcatac tgccaccttt cctcatttta ttatctattt ccccacataa
     4561 cagcatcata caacacaacc gtccgctttt caacaaactt aattaaaaaa atgaaaaaca
     4621 tctgtcggaa acttgtactg taatagatgc caatcaaaaa aatgcaggtt tttcttctta
     4681 ttttacgatt cactatcgaa taaataataa caaatcaccc gcattttttc aacacttttt
     4741 aatatttttg taacatttca aatgttttgt atcatttttc tttatttatt accattcact
     4801 gtttcctata tacaaacaaa ctcatatttc atcagccatc gctcgatcgc ttctttctcg
     4861 cctcggactg ttccctgaac catctcgggt tttccgccgc cgcgtccgtc gaatctttta
     4921 ttaaactctt tcgccatttc acgtacatct tgttgacggc ttcccaacac atatcgatac
     4981 ccgtcttttt catttccaaa gaacactgcg caaatacaat gccctgcgtc taaaacataa
     5041 ttcatcaaca tacgcatgga atcaccgccg aattcttccg taaaaatgca aactgcatct
     5101 ttactttccg aaatcatgtc cgcatacgct ttcactaggc gccactcact tttctgatcg
     5161 ctatttccca attat
//
LOCUS       DBMDXF010000133         5171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_3960_length_5171_cov_2.063116, whole genome shotgun sequence.
ACCESSION   DBMDXF010000133 DBMDXF010000000
VERSION     DBMDXF010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_5171_cov_2.063116"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..1341)
                     /locus_tag="ACZPD4_06165"
     CDS             complement(100..1341)
                     /locus_tag="ACZPD4_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115623313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR1378680.1"
                     /translation="MAREKKPVHKVQMTEGKRNIIHQLLKEYDIQSAEDIQDALKDLL
                     GGTIKEMMEAEMDDHLGYEKSQRSDSGDYRNGYKRKRVNSRYGSMEIEVPQDRKSTFE
                     PQVVKKRQKDISDIDQKIISMYAKGMTTRQISETIEDIYGFETSEGFISDVTDKILPQ
                     IEDWQNRPLDEVYPILYIDAIHYSVRDNGVIRKLAAYVILGINAEGKKEVLTISIGEN
                     ESSKYWLSVLNELKNRGVKDILIICADGLTGIKEAIAAAFPKTEYQRCIVHQVRNTLK
                     YVPDKDRKAFATDLKTIYQAADEKKALAALDRVMEKWTAKYPNSMKRWKDNWDAISPI
                     FKFSAAVRKVIYTTNAIESLNSTYRKLNRQRSVFPSDTALLKALYLATFEATKKWTAT
                     IRNWAQVYGELSIMYEGRLPE"
     gene            complement(1400..1648)
                     /locus_tag="ACZPD4_06170"
     CDS             complement(1400..1648)
                     /locus_tag="ACZPD4_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378681.1"
                     /translation="MMEYVWSTIDFLVMYLFALCFFKPRRRIGKRIDFIILIVVAVMA
                     SFINDYFANSDINLVVTIIFIVVVLGFFEGNENVKLFL"
     gene            complement(1649..2365)
                     /locus_tag="ACZPD4_06175"
     CDS             complement(1649..2365)
                     /locus_tag="ACZPD4_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1378682.1"
                     /translation="MRIAICEDEEFIQNSIEKNVEKCLEITGMAGKCECFVSAEELLE
                     KGNMPYALYILDVEMKKLSGLELAEHIRTEDRNAVIIFMTNHSEMMQKAFDVQAFQYL
                     VKPIDSDTAHNVIMRALRIIREKRNYIRFTNKLKDMIFYYDEVECIESRRRKIIVHTE
                     KRDYEFYGSLSQIEEDIKGSMFVRVHNSYLVNLEKLREVERESVTLRSGLKVPITRTY
                     KQEFNKAYQKFILMSKGGRE"
     gene            complement(2466..3233)
                     /gene="recO"
                     /locus_tag="ACZPD4_06180"
     CDS             complement(2466..3233)
                     /gene="recO"
                     /locus_tag="ACZPD4_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1378683.1"
                     /translation="MEAPAERNGVNQIILTGIVLSTMPVGEYDRRVVILTKEQGKISA
                     FARGARRPNSPLVGALNPFSFGTFTMYEGRTSYTIQSASISNYFAELRGDIIGAYYGF
                     YFLEFTDYYTKEFNDEREMLKLLYQTLRALTNPHIPNMLIRYIFELKALTINGQAPQV
                     FQCMTCGNKERHAVFSAVKGGLVCSECMKDVRDGMSLDPSTLYSMQYIETSTIEKLYT
                     FNVTEKVLTELGNVMSRMTDIYVGKHFKSLEILETLI"
     gene            complement(3441..4337)
                     /gene="era"
                     /locus_tag="ACZPD4_06185"
     CDS             complement(3441..4337)
                     /gene="era"
                     /locus_tag="ACZPD4_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1378684.1"
                     /translation="MREDFKSGFVTLIGRPNVGKSTLMNHLIGQKIAITSNKPQTTRN
                     RIQTVLTTEEGQIVFVDTPGIHKAKNKLGEYMVNIAERSLNEVDVVLWLVEPSAFIGA
                     GDRHIIEQLKKVKTPVVLVINKIDMVKKEEILTFIDAYRKEYDFAEIVPVSARSGDNT
                     DELIKVILKYLPYGPQFYDEDTVTDQPERQIVAELIREKALHCLNEEIPHGIAVAIDR
                     MKAERRVMHIDATIICERDSHKGIIIGKQGSMLKKIGSTARYEIERLLGCKVNLKLWV
                     KVQKNWRDSDYLMKNFGYREDE"
     gene            complement(4522..>5171)
                     /locus_tag="ACZPD4_06190"
     CDS             complement(4522..>5171)
                     /locus_tag="ACZPD4_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1378685.1"
                     /translation="KQLNDADVVQTEAKENRCIIHCKKRNEGFKTSSKVQYVARVGNF
                     IDGGEEYTGALQILKVILSYDYLWQNVRVKGGAYGCMSNFNRIGEGYLISYRDPNLEK
                     TMEIYEGVVDYLENFNVDDRDMNKFIIGTISNIDRPMNPAAKGSRSMNLYMSRVTEGM
                     IRREREQILSADQKDIRALAKILKAVLDADQICVIGSEEKIEEQKEMFMEIRTLS"
BASE COUNT         1331 a         1252 c          789 g         1799 t
ORIGIN      
        1 taaacaaaat cagcttttcg cccttgcttt atatataaca gttacaaaat tgcatgtcaa
       61 cagcaggcgg gtaatccgcc tgtttttctg ctttttttat tactctggca agcgaccttc
      121 atacatgata cttaactctc cataaacctg agcccagttc cggattgttg cagtccattt
      181 ttttgtagct tcaaaggtag ccagataaag ggcttttaaa agggctgtat cgcttggaaa
      241 gacgcttctc tgacggttca gcttccgata agtggaattc agagattcaa tggcatttgt
      301 tgtataaatg acctttctaa ccgctgctga aaacttgaag attggggaaa tagcatccca
      361 gttatccttc cagcgtttca tggaattcgg atattttgca gtccattttt ccattacccg
      421 atccaaagct gccaaagctt tcttttcatc cgctgcctga tatatcgttt tcaaatctgt
      481 ggcaaatgct tttctgtctt tatctggaac atatttcaag gtatttcgca cttgatgaac
      541 gatacatctt tgatattctg tcttaggaaa tgctgccgca atcgcttctt tgatacctgt
      601 gagtccatct gcacaaatga tcaggatatc ttttacacca cgatttttta attcgtttaa
      661 gacggagagc caatacttgg aactttcatt ttctccaata ctgatagtaa gcacttcttt
      721 cttaccttcc gcattaatcc caagaatgac atacgcagca agcttccgga tcactccatt
      781 atcacggacg gaatagtgaa tcgcatcaat atacaggatt ggataaactt catctaatgg
      841 ccgattctgc cagtcttcaa tttgtgggag aatcttgtct gtaacatctg atataaaacc
      901 ttcggatgtt tcaaaaccat aaatatcttc gattgtttct gaaatctgcc gggttgtcat
      961 accttttgca tacatagaaa taatcttctg atcgatatct gaaatgtctt tctgacgttt
     1021 ctttactact tgaggttcaa aagttgattt tctgtcttgt ggaacttcaa tctccataga
     1081 accataacga ctattcactc tttttcgctt ataaccattg cgataatcac cgctatcaga
     1141 acgttgtgat ttttcatatc ccaagtggtc atccatttcc gcttccatca tctctttgat
     1201 ggttccacca agaagatctt tcagcgcatc ctggatatct tctgctgatt ggatatcgta
     1261 ttctttcaag agctggtgaa tgatgttacg ttttccttct gtcatttgta ctttgtgtac
     1321 aggtttcttt tctcttgcca taataaaagg cctccttatg ataaaatatt ttaccataga
     1381 agaccttcta aaacattatt tacagaaaaa gtttcacatt ctcatttcct tcaaaaaatc
     1441 caagtacaac tacgataaaa ataattgtca ccactaaatt aatatccgaa ttggcaaaat
     1501 aatcattgat aaaagatgcc attaccgcca ctacaattaa tattatgaaa tcaattcgct
     1561 ttcctatccg tctcctcggt ttgaaaaagc acaaagcaaa caaatacatt actaaaaaat
     1621 cgattgtact ccatacatat tccatcatct attctcttcc ccctttgctc atcagaataa
     1681 atttttgata tgctttgttg aactcctgct tataagtcct tgtaatcggc actttcaatc
     1741 ccgaacgaag ggtaactgat tcacgctcca cttcccttaa tttttccaaa ttaacaagat
     1801 atgaattatg tactctcaca aacatagatc cttttatatc ttcttcaatc tgactcaaac
     1861 ttccgtaaaa ctcataatct cttttttctg tatgtacgat gatttttctt ctgcggcttt
     1921 cgatgcactc aacctcatca taataaaaaa tcatatcttt caatttgttt gtaaagcgga
     1981 tataatttct tttttcccgg atgatccgca gagcgcgcat aatcacattg tgcgcagtat
     2041 cagaatcgat cggtttcacc agatactgaa acgcctgcac gtcgaatgct ttctgcatca
     2101 tctcactatg atttgtcata aaaatgatca ctgcatttct atcctctgtt cggatatgtt
     2161 ctgccaattc cagtccgctc agcttcttca tctcaacgtc aagaatatac agtgcatatg
     2221 gcatatttcc tttctcaagc aattcttcgg cagacacgaa gcattcacat tttcctgcca
     2281 tccctgttat ttctaaacac ttctctacat ttttttcaat cgaattttgt ataaattctt
     2341 catcttcaca tatcgctatt ctcatttgac cttacgcttt cttctcttcc tttattattt
     2401 tctttcacgt ttttgtctca gacttctgaa atctaagcac aatccctgtc ctgaaataca
     2461 ccacatcaaa taagagtctc taatatttcc agtgatttaa aatgctttcc gacatatata
     2521 tccgtcatcc tgctcatcac atttccaagt tctgtcaata ctttttctgt cacattaaac
     2581 gtatataatt tctcaatcgt acttgtttct atatattgca tactatacaa agtcgaagga
     2641 tccagactca tcccgtcccg cacgtctttc atacattcgc tgcacacaag tccgcctttc
     2701 accgcgctga acacggcatg tctttcttta tttccgcaag tcatacactg aaatacctgc
     2761 ggcgcctgtc cgttgatcgt caacgctttc aattcaaata tgtaacggat aagcatgttt
     2821 ggaatatgcg gatttgtcag cgcccgaagc gtctgataaa gaagttttaa catttcccgc
     2881 tcatcattga attctttcgt atagtaatct gtaaattcca gaaaataaaa accgtaatat
     2941 gctccgataa tgtcgcctct cagctcggca aaataattag aaatacttgc cgactgtatc
     3001 gtgtaagacg tcctcccctc atacatcgta aacgttccga aagaaaacgg gttgagcgca
     3061 ccgacaagcg gactgttcgg gcgtcttgcc ccccgtgcaa acgccgagat ctttccctgt
     3121 tcttttgtaa gtattacaac acgccggtca tactcgccga ccggcatcgt agagagtact
     3181 atccctgtca gtataatctg attcacgccg ttcctttctg ccggagcttc catctgcttt
     3241 ccggctgttt ttataattca aataatcatc aatctttcct gatgtcataa ataattccca
     3301 gtattcttct ttcatctgtt ccacctcttc ttccgtgatc tttaagaata gtttccccga
     3361 cagaaaagaa ggtcatactg tataaaaaat accattttta ttatagaaaa tttttcgatg
     3421 cgatcaccgc tgcgttgata ttactcgtcc tctctgtatc cgaaattttt cataagataa
     3481 tcgctgtcgc gccagttttt ctgtactttt acccacagtt tcaaattcac tttacatccg
     3541 agcagtctct cgatctcgta acgcgcggta cttccaatct ttttcagcat acttccctgt
     3601 tttccgataa taattccttt gtgggaatct ctttcacata tgatcgtcgc gtcgatatgc
     3661 attactcttc gttctgcctt catccgatcg atcgccaccg ctattccgtg aggaatttct
     3721 tcattcagac agtgaagcgc tttttccctg atcagttctg ccacgatctg acgctccggc
     3781 tgatctgtga cagtatcttc atcgtaaaac tgcggtccgt acggaagata ttttaaaatc
     3841 acttttataa gctcatctgt attatctccg gatcttgccg atacaggcac gatctctgca
     3901 aaatcatatt cttttctgta tgcgtcgata aatgtcagta tctcttcttt tttcaccata
     3961 tcgatcttat taataacgag aacaaccgga gttttcactt tcttaagctg ttcgataata
     4021 tgacggtctc ccgccccgat aaaagcagac ggctcgacaa gccaaagcac aacgtcaact
     4081 tcgttcaaag aacgctctgc aatatttacc atatattctc caagcttatt ttttgctttg
     4141 tggatacccg gagtatccac aaaaacaatc tggccttcct ctgtcgttaa aaccgtctgg
     4201 atacgatttc tcgtcgtctg cggtttattc gatgtaatcg cgatcttttg tccgatcaaa
     4261 tgattcatta atgttgactt tcctacgttc ggtcttccga tcaaagttac aaaccctgat
     4321 ttaaaatctt ctctcatatc aatatccttt cccgcgctta ctctttctca tcctgtcttt
     4381 ttattctcac tgcaagcctt ctgcgcttct ctttatcttc cttattgttt ttaatcttat
     4441 cttcttcgtt ttccttgtat ttcctttatc cggtcatccg accgcttcat cattctcttt
     4501 ttcgctgttc tgtcatactt cttagcttaa tgttctgatc tccatgaaca tctctttttg
     4561 ttcttcgatc ttttcctcgc ttccgatcac gcagatttga tctgcgtcaa gcaccgcttt
     4621 taatattttt gcaagcgcgc ggatatcttt ttgatcagcc gaaagaatct gctctctttc
     4681 ccggcggatc attccctctg tcacgcggct catatacaaa ttcatagaac ggcttccttt
     4741 tgcggcagga ttcatcgggc ggtcgatatt actgatcgtt ccgataataa atttattcat
     4801 atctctgtcg tctacattaa agttttcaag ataatctaca acgccttcgt aaatctccat
     4861 cgttttctca agattcggat cgcggtacga gatcagataa ccttctccga tccggttaaa
     4921 attactcata catccataag ctccgccttt cacacggaca ttctgccaca aataatcgta
     4981 gcttaaaatc actttcaaga tctgaagcgc tcctgtatat tcttctccgc cgtcaatgaa
     5041 attgccgact cttgcaacgt actgtacttt tgaagatgtt ttaaatcctt cgtttctttt
     5101 tttacaatga atgatgcatc tgttttcttt cgcttctgtt tgtaccacat ctgcgtcgtt
     5161 tagctgtttt g
//
LOCUS       DBMDXF010000134         5066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4060_length_5066_cov_1.773011, whole genome shotgun sequence.
ACCESSION   DBMDXF010000134 DBMDXF010000000
VERSION     DBMDXF010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4060_length_5066_cov_1.773011"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="ACZPD4_06195"
     CDS             <1..940
                     /locus_tag="ACZPD4_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HIR1378686.1"
                     /translation="AGSLGSTGSSVSALLTQLNEGASDLTDITNSASDDLALIHTSLS
                     DSSTLLSNVSEQLSSASEALLNPNRDGLSVLTDLLAQKPEAIAAFLASPVSLSEHPVY
                     PIENYGSAMAPFYSTLAMWVGGVVLAAMLKVNVSGKALTGVFNPKPHETYIGRSLLFI
                     AIGLLQSSLICLGDLFYLNIQCQHPVYFLFAGWFTSIVYVSIIYALTVSFGDIGKAVC
                     VVLLVMQVAGSGGTFPIEVAPDFFQKVYPLLPFTHSMNAMRECIAGFYSTVYWKELGT
                     LALFLIPALLLGLLLRKPVIRLNDAFTEKLESTKLI"
     gene            complement(1030..1428)
                     /locus_tag="ACZPD4_06200"
     CDS             complement(1030..1428)
                     /locus_tag="ACZPD4_06200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR1378687.1"
                     /translation="MKPSEEIVRFFEKDRFAAETGAVIDEIDDRYARCSLTLEAKHKN
                     ALGAVMGGVYFTLADFAFAVASNWQGDTMVSLNSQIAYLGAAKGNMLIAEAVCLKDGR
                     TTAYYEIRIKDELGNTAAVVSTTGYKLTKL"
     gene            complement(1447..2847)
                     /locus_tag="ACZPD4_06205"
     CDS             complement(1447..2847)
                     /locus_tag="ACZPD4_06205"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1378688.1"
                     /translation="MVEKTMDKIVALAKSRGFVYPGSEIYGGLANTWDYGNLGVELKN
                     NVKKAWWKKFVQENPYNVGVDCAILMNPQTWVASGHLGGFSDPLMDCKECHERFRADK
                     LIEDFCEEKGIELEGSVDGWSQEEMTAFIEEHQIPCPTCGKHNFTDIRQFNLMFKTFQ
                     GVTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKLPFGIAQIGKSFRNEITPGNFTFR
                     TREFEQMELEFFCKPDTDLEWFDYWKNFCLNWLSELGLKEDEVRYRDHDAEELSFYSK
                     ATTDVEFLFPFGWGELWGIADRTDYDLTQHQNVSGQDLTYFDDEAKEKYIPYVIEPSL
                     GADRMVLAFLCSAYDEENIGTEEKPDMRTVLHFHPALAPVKIGVLPLSKKLNEGAEKV
                     FAQLSKYYNCEFDDRGNIGKRYRRQDEIGTPFCVTYDFDSEEDGAVTVRDRDTMEQER
                     VKIEDLKAYFEKKFEW"
     gene            complement(3084..4511)
                     /locus_tag="ACZPD4_06210"
     CDS             complement(3084..4511)
                     /locus_tag="ACZPD4_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIR1378689.1"
                     /translation="MKEQFLWGGATAANQFEGSWDKDGRGTALVDVIPHGENRQAVMD
                     GTFDYRKLPKESYFPGRKAVNGFENYKEDIVLLKELGCKCYRFSISWSRIFPTGEESQ
                     PNENGLNHYENVIDELNKYGIEPIITICHFDIPLALIEKYGSWKSREVIDCYLRYCEA
                     LFHRFGKKVRYWITFNEINMLMHLPFMGAGIRLEEDENPMEVKYQAAHHELVASALAV
                     KMAHEICPGSQVGCMLAAGDVYPYSCDPKDVWASVEKNRENYFFTDIQVRGNYPSYAE
                     KLFERENIRLKMKENDLEILKENTVDFVSLSYYNSRCVRADGKGEAAGGNVFASAKNP
                     HLTNSQWGWPIDPLGLRITLNTLYDRYQKPLFIVENGLGAKDTPDENGFVDDDYRIEY
                     LSSHVKAMMDAVEKDGVDLIGYTAWGWIDLVSATTGEMSKRYGMVYVDLDDFGKGTGK
                     RIPKKSFYWYKNVIESNGDCLRDDA"
     gene            complement(4528..>5066)
                     /locus_tag="ACZPD4_06215"
     CDS             complement(4528..>5066)
                     /locus_tag="ACZPD4_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1378690.1"
                     /translation="NREKEDKIGKIEKFDKNESDKIDRKERKTEKISSPLKGEVIALS
                     DVQDEAFSSGALGLGAAVDPKEGVLYAPADGTISVFFPTGHAIGMTTDDGVELLIHVG
                     MDTVQLEGKGFRPFAKEGDRVTKGQKLLEFDMELICKEGYSLVTPVLITNTDEFSDIS
                     ITSECHIDPQSILMTVTR"
BASE COUNT         1268 a         1346 c          890 g         1562 t
ORIGIN      
        1 ggcaggcagt ctaggcagca ccggatcttc tgtatccgct cttttaacac aattaaatga
       61 aggcgcctct gacttgacag atattacaaa cagcgcttcc gatgaccttg cccttatcca
      121 tacttcgctt tcagattcct caactttgct aagtaatgta tctgaacaac tttcatccgc
      181 gtctgaagcg ctcttaaacc caaaccgtga cggactttcc gtgttgaccg acttgttggc
      241 gcaaaaaccg gaagcaatcg ccgcctttct tgcgtctccg gtcagtcttt ccgagcatcc
      301 ggtctacccg atcgaaaatt acggatcggc aatggctcct ttttattcta cactcgccat
      361 gtgggttggc ggcgttgtgc ttgccgccat gctgaaagtc aatgtttcag gaaaagcttt
      421 gacaggagtt tttaatccga aacctcatga aacatatatc ggaagaagtc tactttttat
      481 agcgatcggc cttttgcaaa gcagcctcat ctgtctgggc gatttgtttt atttaaacat
      541 ccagtgtcaa catccggttt acttcctttt cgccggatgg tttacaagca tcgtgtatgt
      601 aagtatcatt tatgcgctca ccgtttcatt cggcgatatc ggaaaagccg tctgcgtcgt
      661 cttacttgtt atgcaggtag ccggaagcgg cggaacattc cctattgagg tagcgcctga
      721 ctttttccaa aaagtctatc cccttctgcc gtttacgcac agcatgaatg ccatgagaga
      781 atgtattgcc ggcttttaca gcacagttta ttggaaagaa ctcggaacat tagctctttt
      841 tctcatcccc gcactattgc tcggtctgct ccttcggaaa cctgtcatac gtctgaatga
      901 tgcatttacg gagaagctcg aaagcacaaa acttatctga atgcaaaaat ttccgcatgg
      961 atcttctcca cgcggaaatt tttatttttt ctctttattc tttaaaaagt tttacccgac
     1021 ggctgttctt cacaatttcg taagcttgta acctgtcgtg ctcacgactg ccgccgtatt
     1081 cccgagttca tccttgatac gaatctcata atacgccgtt gtcctgccgt cttttaaaca
     1141 gactgcttct gcaatcaaca tattcccttt tgccgctcca agataagcaa tctgtgagtt
     1201 caatgatacc attgtatcgc cctgccagtt tgaagcaact gcaaacgcaa aatcggcaag
     1261 tgtaaaataa acgcctccca ttactgcccc aagcgcattc ttatgttttg cctctaatgt
     1321 cagactgcat ctcgcatagc ggtcgtctat ctcgtctatc accgccccgg tctctgccgc
     1381 aaaccgatct ttctcaaaaa aacggacgat ttcttctgat ggtttcattg ttcctttgct
     1441 tccttcttac cactcaaatt ttttctcgaa gtatgctttc aaatcttcga tcttcactct
     1501 ctcctgctcc atcgtatcac gatcacgcac tgtaacagct ccgtcttctt ccgaatcaaa
     1561 gtcatatgtt acacagaacg gagtaccgat ctcatcctga cggcgatatc tctttccgat
     1621 atttccacga tcgtcaaact cgcagttata atatttgctc aactgtgcaa atactttctc
     1681 cgcgccttca ttcaatttct tagacagcgg aagaacgccg atcttaaccg gagcaagcgc
     1741 cggatggaaa tgaagaacgg tacgcatatc cggtttctct tctgttccga tattctcttc
     1801 gtcatatgca ctgcaaagga acgccaatac catacggtct gcgccaagtg acggctcaat
     1861 tacatacgga atgtactttt ctttcgcttc atcgtcaaaa taagtaagat cctgtcccga
     1921 tacattctga tgctgtgtca ggtcatagtc tgtacggtct gcgattcccc aaagttctcc
     1981 ccatccaaac gggaacaaga actctacgtc tgtcgtcgct ttactgtaga aagacaactc
     2041 ttccgcatca tggtcacggt aacgtacttc gtcttctttc agtccgagtt cagacagcca
     2101 gttcagacag aagtttttcc aataatcaaa ccattccaaa tctgtatccg gtttgcagaa
     2161 gaactcaagc tccatctgct caaactctct cgtacggaat gtaaagttac ccggtgtgat
     2221 ctcgtttctg aaagattttc cgatctgagc gataccaaac ggaagtttct ttcttgatgt
     2281 acgctgtacg tttttaaagt ttacgaaaat tccctgcgcc gtctccggtc tgagataaac
     2341 cgtattcttc gcatcttctg taactccctg gaatgttttg aacatcaggt tgaactgacg
     2401 gatatctgta aaattatgtt ttccgcatgt cggacacgga atctgatgct cttcgataaa
     2461 tgccgtcatc tcttcctgag accatccgtc cacagaacct tccagttcaa ttcctttttc
     2521 ttcgcagaaa tcttcgatca atttatccgc gcggaaacgc tcatgacatt ctttacaatc
     2581 cataagcgga tcgctgaaac ctccgagatg accggaagcc acccaagtct gcgggttcat
     2641 aaggatcgcg cagtcgacac cgacattgta tgggttctcc tgtacaaact tcttccacca
     2701 tgcttttttt acattgtttt tcagttctac accgagattg ccgtaatccc atgtattcgc
     2761 cagaccgccg taaatctctg atcccggata tacgaatcct cttgattttg caagcgccac
     2821 gattttatcc attgtctttt caaccatttt tatgctcctc acatttttta ttgtaacagt
     2881 tcagccatac agatgtacgg gaagcagaaa acatgcctgc atgtttttgc aagcggacat
     2941 ctgtatgtgc ttagcgcctt tttatgagta aaacagtagc ggcagctaca gtaagcacgc
     3001 agtgcggttt tacgaatggc gcaggctgaa ctattacttt ttattctcga tggtattcat
     3061 tataacttgt gcggccttat ttttcaagca tcatcgcgca gacaatctcc attgctctct
     3121 atcacatttt tataccaata aaacgatttt ttcggaatcc gttttcccgt tccttttccg
     3181 aagtcgtcca ggtccacata aaccatccca tatcttttcg acatctctcc ggtcgttgca
     3241 ctgaccagat cgatccaccc ccatgcagta tacccgatca gatctacacc gtccttttcc
     3301 actgcatcca tcattgcttt cacatgggaa gaaagatatt ctattctata atcatcatca
     3361 acaaacccgt tctcatccgg cgtatctttc gctccaagtc cgttttccac aataaacaat
     3421 ggtttttgat atctgtcgta cagcgtgttt aacgtaatcc tcagtccgag aggatcgatc
     3481 ggccatcccc actgactgtt ggtaagatgc gggtttttcg cagaagcaaa cacgtttccc
     3541 cctgcggctt cgccttttcc gtccgctctc acacagcggc tgttataata cgaaagcgaa
     3601 acaaaatcaa ccgtattctc ttttaagatt tcaagatcat tctccttcat cttcagccga
     3661 atattttccc gttcaaataa tttttcagca taagaaggat aatttcccct gacttgtatg
     3721 tctgtaaaaa agtaattttc tctgttcttt tccacgcttg cccatacgtc tttcggatca
     3781 cagctatacg gatagacgtc tcccgccgca agcatacatc cgacctgtga gcccgggcat
     3841 atctcatgcg ccattttcac agccagtgca gaagcaacaa gttcatgatg agctgcctga
     3901 tattttactt ccattggatt ctcatcttct tccagacgaa tacctgctcc cataaacggc
     3961 agatgcatca gcatattaat ttcattaaat gtaatccaat accggacttt tttcccgaaa
     4021 cggtgaaaca aagcttcaca gtaacgaaga taacaatcga tcacttcgcg gcttttccat
     4081 gaaccatact tctctataag agccagcgga atatcaaaat gacagattgt tataatcggt
     4141 tcaattccat acttattcag ctcatcgatc acattctcat aatgattcaa tccattctca
     4201 ttcggctgtg attcctctcc ggtcggaaaa atcctcgacc atgaaattga gaaacggtaa
     4261 catttacatc ccagttcttt taaaagcaca atgtcttctt tataattttc aaaaccgttg
     4321 accgcttttc ttcccggaaa ataagattcc ttcggaagtt tcctataatc gaacgttcca
     4381 tccatgacag cctgacgatt ctctccgtga gggatcacat caacgagtgc agtccctctt
     4441 ccatctttat cccagcttcc ttcaaactga tttgccgctg ttgctccgcc ccacaaaaac
     4501 tgttctttca tacgcaacaa tcctttccta tcgagtaact gtcatcaaga tcgattgcgg
     4561 atctatatga cattcacttg taattgaaat atctgaaaat tcatctgtat ttgtaataag
     4621 aaccggagtg acaagagagt acccttcctt acaaatcagt tccatatcaa attctaaaag
     4681 cttctgtcct ttcgttacgc gatctccttc tttggcgaat ggacggaatc cctttccttc
     4741 caactgtacc gtatccattc cgacatggat cagcaattcc actccgtcgt ccgttgtcat
     4801 cccgattgca tgacctgtcg gaaaaaacac agagattgtt ccgtctgccg gtgcatacaa
     4861 aactccttct ttcgggtcaa ctgccgctcc aagtccaagc gctcccgatg aaaaagcttc
     4921 gtcctgaaca tctgaaagcg ctataacttc tcccttcaac ggactgctta ttttctctgt
     4981 tttcctttct ttcctgtcaa tcttatccga ttcattctta tcaaacttct caatcttacc
     5041 gatcttatct tctttctctc tattct
//
LOCUS       DBMDXF010000135         5039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4088_length_5039_cov_1.929729, whole genome shotgun sequence.
ACCESSION   DBMDXF010000135 DBMDXF010000000
VERSION     DBMDXF010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4088_length_5039_cov_1.929729"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            91..813
                     /locus_tag="ACZPD4_06220"
     CDS             91..813
                     /locus_tag="ACZPD4_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378691.1"
                     /translation="MKKKTLLIKTADLCIRIHYKYPYIEQQCETWRVNEGYEPDIEVS
                     VTDEEIETEYAGYRAESVSRALCESTCIYRAIARKLIAFQAFVMHGAVLELDGKAYVF
                     TAKSGVGKTTHTKLWVEYFEGRASYINGDKPIIRCKDGVWYAYGTPWMGKEKFGSQSS
                     APIQAVCFIERGEENKIQKIADKEVIDRVFHQLFFPEDPETLIEFMGLADDFVQKLPF
                     FVLKCNISAEAVRVAYETLSKV"
     gene            871..1137
                     /locus_tag="ACZPD4_06225"
     CDS             871..1137
                     /locus_tag="ACZPD4_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR1378692.1"
                     /translation="MKIKEGFIKRQVGERVVVVPIGEAAKNFHGMINLNETGGELWDL
                     LKENTDEDKLLESMLEIYEIEPEQAQKSMKTFLRKLREAGVLEE"
     gene            1251..2690
                     /locus_tag="ACZPD4_06230"
     CDS             1251..2690
                     /locus_tag="ACZPD4_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR1378693.1"
                     /translation="MSYNATGNSAGRRIATLLDEGSFVEIGGAVTARSTDFNLQEKET
                     PADGVITGYGVIDGNLVYVYSQDAAVLNGAIGEMHAKKIANIYDMAMKMGAPVIGLID
                     CAGLRLQEATDALEAFGSVYYKQAMASGVVPQIAAIFGMCGGGLAVVPGLADFTFMEE
                     KSGKLFVNSPNALAGNEISRCDTSAAKYQSETAGLVDGIGTEADILNEIRSLICMIPA
                     NNEDDLSYEECMDDLNRICPDVENAAADTAIVLSQIADDQIFTEMKKDYAKEMVTGFI
                     RLNGMTVGAVANRTKVYAEDGTEAASFDCALTVDGCKKAVDFVNFCDAFSIPVLTLTN
                     VEGFSATLDSEKDMARAVARMTYAFAEATVPKVNVIIGNAYGSAYIAMNSKSIGADMV
                     YAWENAKIGMMDANLAARIMYDDADMETQKEKAAEYEKIQSSAKSAARRGYVDAIITP
                     ADTRKYVIGAFEMLFTKREDRPNKKHGTV"
     gene            2705..3484
                     /locus_tag="ACZPD4_06235"
     CDS             2705..3484
                     /locus_tag="ACZPD4_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HIR1378694.1"
                     /translation="MKKKISLLGLILILVFSFSGCGKSDDTVEYDQEQLEQYADFIVQ
                     NFSMMGEADFQNFSDMSDLELEMTLLQAGVPVTGENFLTMMGAWDAGEQECGEFVGLK
                     DGYTMETTNEGALLTVEGDFAERDGALEFAFDKKGNIESLTIGAHYSTAEILKKAGLN
                     TILGMGTVFVVLIFISFIISLFKFIPALEQKFKKSAKTEIVNEKPTAALKTEMPEVSE
                     DVTEDTELAAVIAAAIAASEGTSADGFIVRSIKRRKSNKWN"
     gene            3503..3871
                     /locus_tag="ACZPD4_06240"
     CDS             3503..3871
                     /locus_tag="ACZPD4_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR1378695.1"
                     /translation="MKNYTITVNGNVYDVTVEEKGAGAVPALKPAPKAAPAPKAAPAP
                     VAKAAGAGNIQVKAGAAGKVFQIPTSVGQSVQQGDTVIVIEAMKMEIPVVAPEAGTIA
                     SIDVAVGDAIESGAVLATLN"
     gene            3895..>5039
                     /locus_tag="ACZPD4_06245"
     CDS             3895..>5039
                     /locus_tag="ACZPD4_06245"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1378696.1"
                     /translation="MEYISNTLGNLIEQTAFMNLTVGNLIMIAVACVFLYLAIRHGFE
                     PLLLVPIAFGMLLVNIYPDIMLHAEDSANGTGGLLYYFYVLDEWSILPSLIFLGVGAM
                     TDFGPLIANPKSFLLGAAAQFGIFAAYLGAMAMGFSDKAAAAISIIGGADGPTSIFLA
                     GKLQQTAILGPIAVAAYSYMSLVPIIQPPIMKLLTTEKERKIKMEQLRPVSKLERILF
                     PIIVTIVVCTILPTTAPLVGMLMLGNLFKESGVVRQLTETASNALMYIVVILLGTSVG
                     ATTSAEAFLNWDTLKIVALGLIAFAFGTAAGVLFGKIMCWATKGKVNPLIGSAGVSAV
                     PMAARVSQKVGAEADPTNFLLMHAMGSHTHSLFHSCYFICHIICAVKS"
BASE COUNT         1517 a          846 c         1333 g         1343 t
ORIGIN      
        1 agagtcttgt cgaacctttc ttttttttgt atactttagt agattataaa ttatgagaga
       61 gtgtgaaaag tagcagggaa gggaaatgat atgaagaaga aaacactgct tattaaaaca
      121 gcagatttat gtatccgaat tcattataaa tatccgtata ttgaacaaca atgtgaaaca
      181 tggagggtga atgaaggata cgaaccggat attgaagtgt ctgttacaga tgaagaaatt
      241 gaaacggaat acgcggggta tcgtgcagaa agcgtgagca gagctttgtg tgagagtaca
      301 tgtatttacc gtgcgatcgc aagaaagctg attgcctttc aggcatttgt tatgcacggc
      361 gccgtattgg agttagacgg aaaagcgtat gtatttaccg cgaagagcgg agtcggaaag
      421 acaacacata cgaagctgtg ggtcgagtat tttgaaggca gagcaagtta tattaacggg
      481 gataagccga tcatcagatg taaagacggc gtatggtatg catatggcac accgtggatg
      541 ggaaaagaaa agtttggaag tcaaagttct gcgccgattc aggcagtttg ctttattgaa
      601 cggggagaag aaaataagat tcaaaagatt gctgataagg aagttataga cagagtgttt
      661 catcaactgt ttttccctga agatcctgag acgttgatcg agttcatggg attggcagac
      721 gattttgttc aaaagcttcc gttttttgta ttaaagtgca atatttcagc agaagctgta
      781 cgggtggctt atgagacttt gagtaaagta taagtttgaa gaaacacaat taaggcgata
      841 aaaaagaaat aaaatcagga gaatacgaag atgaaaataa aagaaggatt tatcaaacgt
      901 caggtaggag aacgcgtcgt agtcgttcca attggagaag cggcaaaaaa ctttcatggt
      961 atgatcaacc ttaatgagac aggcggagaa ttgtgggatc ttctgaaaga aaatacagat
     1021 gaggataagt tgttagagtc catgctggag atttatgaga ttgaacctga gcaggcgcaa
     1081 aaaagcatga aaacttttct gagaaaatta agagaggcgg gggttttgga ggaataaaga
     1141 gaaagccttg cagtaagttt taattgaaat cttatagatg ttagtatata ctaaaatagg
     1201 cagaaatttt ggtttattta aacaaattta tttatatgga gggctaaaat atgagctaca
     1261 atgcaacagg aaactcagcg ggcagaagaa ttgctacatt gcttgatgag ggaagctttg
     1321 ttgagatcgg cggcgcagtt actgccagaa gcacagattt caacttgcag gaaaaagaaa
     1381 ctccggctga tggtgttatt accggttatg gagtgattga cggcaatctt gtgtatgtgt
     1441 acagtcagga tgccgcagtg ctcaacggag cgattggaga gatgcatgca aagaagatcg
     1501 caaatatcta tgatatggcg atgaagatgg gagctcctgt catcggtctt atcgattgtg
     1561 ccggattgag acttcaggaa gctacggatg ctttagaagc ttttggaagt gtttactaca
     1621 agcaggcaat ggcatcggga gttgttccgc agattgcggc gattttcggt atgtgcggcg
     1681 gcggacttgc agttgttccg ggacttgctg actttacatt catggaagaa aaaagcggaa
     1741 agctttttgt aaattctccg aatgcgcttg caggaaacga gatttcaaga tgtgatactt
     1801 ccgctgcaaa ataccagagt gagacggcag gtcttgtcga cggtatcgga acagaagcgg
     1861 atattttaaa tgaaattcgt tctttgatct gcatgatccc ggcaaataat gaagatgatc
     1921 tgtcctatga agaatgtatg gacgatctga atcgtatttg tccggatgtt gaaaatgcgg
     1981 cagccgatac tgcaatcgtt ctttcacaga ttgcagatga tcagattttt acagagatga
     2041 agaaagatta cgcaaaagaa atggtgacag ggtttattcg tctgaacggt atgacggtcg
     2101 gggctgttgc gaatcgtaca aaagtttacg cagaagacgg aacagaggca gcatcatttg
     2161 attgtgcact tacagtggat ggctgtaaga aagcggttga ctttgtgaat ttctgtgatg
     2221 cattctcgat tccggtgctc acattgacaa atgtagaagg attttcagct acgctcgact
     2281 ctgaaaaaga tatggcaaga gctgtggcaa gaatgactta tgcgtttgct gaggcaacag
     2341 tgccgaaagt gaatgtaatt ataggaaatg cttatggaag cgcatacatc gcgatgaaca
     2401 gcaaatcaat cggtgcggac atggtatatg catgggaaaa tgcaaagatc ggtatgatgg
     2461 atgcaaacct tgcggctcgt atcatgtatg atgacgcgga catggaaaca caaaaagaga
     2521 aagcggcaga atatgaaaaa atccaatcca gtgctaaatc agcggctcgc agaggatatg
     2581 ttgatgcgat catcacgcct gccgatacga gaaaatatgt gatcggcgca tttgagatgc
     2641 tgtttacaaa gagagaggat cgtccgaata agaaacacgg tacggtttag tgaggtgagg
     2701 cgaagtgaag aaaaaaatca gtttattagg gcttatcctc attcttgttt ttagtttttc
     2761 gggatgcgga aagtctgatg atacagtaga atatgatcag gaacagcttg agcagtatgc
     2821 cgattttata gttcagaatt tcagtatgat gggagaggcg gattttcaga acttttccga
     2881 tatgtctgat cttgaacttg aaatgactct tcttcaggca ggcgtacctg taacaggaga
     2941 aaatttcctg acgatgatgg gggcgtggga tgccggagaa caagaatgcg gagagtttgt
     3001 cggcttaaaa gacggttata caatggaaac aacaaatgaa ggcgcacttc tgactgttga
     3061 aggagatttt gcggaaagag acggagcgct tgaatttgcg tttgataaga aaggaaatat
     3121 cgagagtctg acgatcggag cacattacag tacggcggag attttgaaaa aagcaggact
     3181 taatacgatt ctcggaatgg gaacggtttt tgttgtattg atctttattt catttattat
     3241 ttcactgttt aaatttattc cggcgcttga acagaagttt aaaaagagtg caaaaacaga
     3301 gattgtaaat gaaaaaccaa cggcagcttt gaaaacggag atgccggaag tgtcggaaga
     3361 tgttacggag gatacagagc ttgcagcggt aattgcggcg gcaatcgcgg catctgaagg
     3421 aacatcggca gacggattta tcgttcgttc tataaagaga agaaaatcaa ataaatggaa
     3481 ttaaagaaaa ggagataaga aaatgaaaaa ttatacaatt acagtaaatg ggaatgtata
     3541 tgatgtaaca gtggaagaaa aaggcgcagg cgctgttccg gctttgaaac cggctccgaa
     3601 agcagctccg gctccgaaag cggcacctgc tccagtggca aaagcagcgg gcgcaggaaa
     3661 cattcaggtg aaagcaggcg cggcaggaaa agtattccag atcccgacaa gcgtaggtca
     3721 gagtgttcaa cagggtgata cagtgatcgt gatcgaggca atgaagatgg aaattccggt
     3781 tgttgcaccg gaagcgggaa cgatcgcaag tattgatgtg gctgtaggag atgcgataga
     3841 atcaggagca gtattagcta cgttaaatta gttactgacg gaggttttta acaaatggaa
     3901 tatatttcaa atacactggg aaacctcatt gaacagacag cgtttatgaa tcttacggtc
     3961 ggaaacctga tcatgatcgc agttgcgtgt gtgtttctct atttagcaat cagacacggc
     4021 tttgagccgc ttctgcttgt accgattgct ttcggtatgc tgcttgtaaa tatctatcct
     4081 gatattatgc ttcatgccga ggactcagcg aatggtacgg gtggattact ttattacttc
     4141 tatgtccttg acgagtggtc gatccttccg tctttgatct tcctcggtgt aggagcaatg
     4201 acggatttcg gacctttgat cgcgaacccg aaaagtttcc ttctcggagc tgccgctcag
     4261 tttggtatct ttgctgcata tttaggcgct atggcaatgg gattttcaga taaagctgcc
     4321 gcagcgatct ccattatcgg aggagctgac ggaccgacat ctattttcct tgccggaaaa
     4381 ctgcaacaga cagcgattct cggaccgatc gcggtagcgg catattctta tatgtcactt
     4441 gtaccgatca tccagccgcc gatcatgaaa cttcttacga cggagaaaga acgtaagatc
     4501 aagatggagc agcttcgtcc ggtatcaaaa ttagagagaa ttcttttccc gatcattgta
     4561 acgatcgttg tttgtacgat tcttccgacg acagctcctc ttgtaggtat gcttatgctc
     4621 ggaaacttgt ttaaagagtc cggtgttgta agacagctta cagagaccgc ttctaatgcg
     4681 ttgatgtata tcgttgtaat tttgctcgga acatctgtgg gagcgacaac aagcgctgag
     4741 gcgttcttga attgggatac tttaaaaatc gttgctctcg gactgattgc atttgcgttc
     4801 ggtacagcgg cgggagtgtt gttcggtaaa atcatgtgct gggcaacgaa aggaaaagtc
     4861 aatccgctga ttgggtctgc gggcgtatct gcagttccta tggcggcgcg tgtttcacag
     4921 aaagtgggag ccgaggctga tccgactaac ttcttattga tgcatgcgat gggttctcat
     4981 acccattcgc tttttcatag ctgttatttc atctgccata tcatatgcgc tgttaaatc
//
LOCUS       DBMDXF010000136         5001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4118_length_5001_cov_2.107143, whole genome shotgun sequence.
ACCESSION   DBMDXF010000136 DBMDXF010000000
VERSION     DBMDXF010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_5001_cov_2.107143"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="ACZPD4_06250"
     CDS             complement(<1..805)
                     /locus_tag="ACZPD4_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR1378697.1"
                     /translation="MKRNRKLAVVICICLILSLCLPFFLSGCRKKKIDSEMSVYYLNE
                     DRTGLVKAPYETGKTAKGKKMTDKEICGMAEDILETLRKPSDKIENVPPIPNEVSVQK
                     CELRGSILDIDFNKAYLKVNSLEEKLMRASIVCSLSEIEGVNAVLFTIDGESLKDSDG
                     NSIGLMTEDDFVENTGSSPSAYQTVELTLYFANESGDKLVPQKVSARYSSNLSKEKMI
                     VEKLMQGPESGAYPTINPNANLLGVTIKDDICYVNFDSTFLNGEYDILPK"
     gene            complement(802..2295)
                     /locus_tag="ACZPD4_06255"
     CDS             complement(802..2295)
                     /locus_tag="ACZPD4_06255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378698.1"
                     /translation="MYIQSGEWVIISKIKYQFVSNRKIIGNMRFCVILCTMLAGLIPC
                     LATLFGIVAFYESRAVELRTAEIQNQCTILSNQLSAYGYLSDPTSEVIDSNLTQLTNI
                     YNGRVMIMDEDLCVIKDTYNLDEGKTNVSESVVRCMRTKPTNHYDKKNHYIEVTSPIN
                     IPGKKKIDGVILASVSTDSIEVTKQVFYTHGGVVVGIAAIFLAVFGVALANKLVKPIH
                     KITGAIENVSEGYSDDVLHVDTFAETSQLSEAFNKMLGRLKQLDDSREEFVSNVSHEL
                     KTPMTSMKVLADSLLEQENLPVEMYREFMEDIAKEIDRENQIITDLLSLVKMNRTGQT
                     MNIQQVNINDLLEQILKRLKPIAEKKNVEMVMESFRPVSAEIDETKFTLAVSNLVENA
                     IKYNHENGWVHVSLNADHKYFYLKVEDSGLGIPQEDQAHIFERFYRVDKSHSREIGGT
                     GLGLAIARNAVIVHRGSIKVHSNEGEGTTFTVRIPLIYTTVPEGVGR"
     gene            complement(2252..2938)
                     /locus_tag="ACZPD4_06260"
     CDS             complement(2252..2938)
                     /locus_tag="ACZPD4_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378699.1"
                     /translation="MGKRILVVDDEKLIVKGIRFSLEQEGMEVDCAYDGEEALEYAKA
                     CEYDLVLLDVMLPKLDGFQVCQQIREFSDMPVVMLTAKSEDMDKILGLEYGADDYITK
                     PFNILEVKARIKAIMRRTSKRKEPAGGPVLIKGSLKIDCESRRVFIGEREINLTAKEF
                     DVLELLAMNPNKVYSRENLLNLVWGYDYPGDARTVDVHIRRLREKIEMNPSEPKYVHT
                     KWGVGYYFQD"
     gene            complement(2994..3671)
                     /locus_tag="ACZPD4_06265"
     CDS             complement(2994..3671)
                     /locus_tag="ACZPD4_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1378700.1"
                     /translation="MQDNQKKYRKKYAVMTVFFLISASFLFGCGNKDSAVPETEEFLF
                     SAEEEKSDHTSQKSEEPAEKAERKKAEADSGTVEENPERKKVFVYVCGEVQASGVYEL
                     EQDSRVFEAIAKAGGLTENAAAEAVNQARVVVDGEQIYVPSLDEVKGAGVVSEVTERT
                     DDGKININTAEKEELMTLTGVGEAKAQSIIAYREEHGGFQSIEELMQIEGIKEGVFNK
                     IKEDITI"
     gene            complement(3850..4281)
                     /locus_tag="ACZPD4_06270"
     CDS             complement(3850..4281)
                     /locus_tag="ACZPD4_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1378701.1"
                     /translation="MIGFLIALLSGALMSIQGVFNTQVTKTTGMWVGNGWVQFTAFAV
                     CLAMWFFTGRDQVSQLMEVQPKYMLLGGVIGAGITWTVIKSIEALGPARSALLIVIAQ
                     LTVAYLIQLFGLFGMDREPFSWRKAGGLLLAVVGIAVFQWK"
     gene            complement(4422..>5001)
                     /locus_tag="ACZPD4_06275"
     CDS             complement(4422..>5001)
                     /locus_tag="ACZPD4_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR1378702.1"
                     /translation="EFMGGIRKLCDENDILMICDEVQCGMGRTGTMFAWQGYGVKPDI
                     LTMAKAIGNGIPVGAFAMTEEVAGYSLKPGDHGATYGGNPLACTAVKTVLSIFERKNI
                     VGHVNEIAPYFTEKLEELKAEFDCVTERRGKGLMQGLVITKPIKEINDRAIEEGLLII
                     QAQGNVIRFVPPLIVEKEHIDQMVEKMRRILA"
BASE COUNT         1242 a         1335 c          832 g         1592 t
ORIGIN      
        1 gtttcggcag gatatcatac tctccgttca gaaacgtact atcgaaattc acataacaaa
       61 tatcatcttt gatcgtcact cccaacagat ttgcattcgg gttgatcgtc ggataagctc
      121 cgctttcagg accctgcatc agtttctcaa cgatcatttt ctctttcgac aaattactgc
      181 tgtaccttgc cgaaactttt tgcggaacaa gcttatctcc gctttcattt gcaaaataaa
      241 gcgttaattc taccgtctgg taggcgctcg gtgacgatcc tgtgttttct acgaaatcat
      301 cttctgtcat aagtccgatc gaattgccgt cactgtcttt aagcgactct ccgtcgatcg
      361 taaacagtac tgcgttgacc ccttcaattt ccgaaagcga acatacaata gatgccctca
      421 taagcttttc ttccagactg tttactttta aatacgcttt attgaagtcg atatctaata
      481 tactcccccg cagttcacat ttttgtacag atacttcatt cggaatcgga ggaacatttt
      541 cgattttatc cgatggtttc cgaagcgttt ccagtatatc ctctgccata ccgcagattt
      601 ctttgtccgt catcttcttg ccttttgccg ttttcccggt ttcatagggc gctttaacaa
      661 gtcctgttct gtcttcatta agataataga ctgacatctc cgaatctatt ttcttcttcc
      721 ggcagcctga aaggaaaaac ggcagacaca aggacaaaat aagacaaatg catattacga
      781 ccgcaagttt cctgttcctt ttcatctccc cactccttcc ggcactgttg tatagatcag
      841 cggaatccga actgtaaatg tcgttccctc gccttcgtta ctatgcactt tgatcgatcc
      901 tctgtgaacg atcactgcgt ttctggcgat cgcaagcccc agtcccgttc cgccgatttc
      961 tctggaatga gacttgtcta cccggtaaaa cctctcaaat atatgtgcct gatcttcctg
     1021 agggattcca agaccggaat cttctacttt taaataaaaa tatttatgat cggcatttaa
     1081 agacacatga acccatccgt tttcatgatt atatttaatc gcattttcca caagatttga
     1141 aacggcaagt gtaaatttcg tctcatcaat ctctgcgctc accggacgga aactttccat
     1201 gaccatctca acatttttct tttccgcgat cggtttcagc ctctttaata tttgttcaag
     1261 caagtcgttg atattcacct gctgaatgtt catcgtctga ccggtccgat tcattttcac
     1321 aagagaaaga agatctgtta tgatctgatt ttcccggtcg atttctttcg cgatatcttc
     1381 cataaattca cggtacattt ccacaggaag gttttcctgc tcaagaagcg aatctgccag
     1441 cactttcatc gatgtcatcg gagttttcag ttcatgggaa acgtttgaga caaactcttc
     1501 tctggagtcg tccagctgct ttagtctgcc gagcattttg ttgaacgctt cacttagctg
     1561 acttgtctcc gcaaacgtgt ctacgtgcag cacatcgtca ctgtatcctt cactcacatt
     1621 ttcaatcgcc cctgtaatct tatgaatcgg ttttacaagt ttattggcaa gggccacccc
     1681 gaaaacagcc aagaaaatcg ccgctattcc tactacgaca cctccatgcg tataaaatac
     1741 ctgttttgta acttcaatac tgtctgtaga gacacttgct aaaatcacac cgtctatctt
     1801 ctttttcccc gggatattga tcggtgaagt cacttcgata taatgattct ttttatcata
     1861 gtggttcgtc ggctttgtgc gcatgcaccg aacgacgctt tccgatacat tcgtctttcc
     1921 ttcatcgaga ttatatgtat ctttaattac gcagagatct tcatccatga tcataactct
     1981 gccgttatat atattcgtca gctgcgtcag attagaatcg atcacttccg aagtcggatc
     2041 ggaaagatat ccgtatgccg agagctgatt gcttaatatt gtacattgat tttggatttc
     2101 cgcagtccga agttcgaccg cacgcgactc gtaaaatgct acgattccga aaagtgttgc
     2161 aaggcagggt atcagccctg ccagcattgt acaaagaatc acacaaaaac gcatgttccc
     2221 gataatttta cggttggata caaattgata tttaatcttg gaaataataa cccactcccc
     2281 actttgtatg tacatatttc ggttcactcg gattcatctc aatcttctca cgaagccttc
     2341 ggatatgtac gtctactgtt cttgcatctc ccgggtaatc atatccccac accagattca
     2401 gcagattttc tctgctgtat actttgttcg gattcattgc cagaagctct aaaacatcaa
     2461 attcttttgc cgtaagattg atctctctct ctccgataaa tactcttctg ctctcacaat
     2521 caatctttaa gcttcctttg atcagcaccg gtccgcctgc cggttctttc cgtttcgacg
     2581 tccgcctcat gatggctttg atcctcgcct tcacctctaa aatgttaaac ggtttcgtta
     2641 tataatcatc cgcaccgtat tccagtccca gtatcttgtc catatcctcg ctctttgccg
     2701 tcagcatcac aaccggcata tccgaaaatt cccgaatctg ctgacacacc tgaaaaccgt
     2761 ccagtttcgg cagcatcaca tccaaaagga caagatcata ttcacatgcc tttgcgtatt
     2821 ccaaagcctc ctcgccgtca taggcgcagt ctacttccat gccttcctgc tcgaggctga
     2881 agcggattcc ttttacgatc agcttctcat catcaacaac taaaattctc ttacccatac
     2941 tcctcttttt tcgactcctt atgcactgcg gtatacaaaa ccgctctctt atgtcagatc
     3001 gttatgtctt cttttatttt attaaatact ccctctttga ttccctcaat ctgcatcaat
     3061 tcttcgattg actgaaagcc gccgtgttct tcccgatatg cgattatact ttgcgctttc
     3121 gcctctccga ctcctgtaag cgtcatgagt tcctcttttt cggctgtatt aatatttatc
     3181 ttcccatcgt ccgtacgttc ggtcacttcc gatacaaccc ctgcgccttt cacttcatct
     3241 aaagaaggca cataaatctg ttctccgtca acgacaaccc tcgcctgatt aaccgcttcc
     3301 gccgcagcgt tttctgtcag cccgcccgct ttcgcaatcg cttcaaacac tctggaatcc
     3361 tgttccagtt catatacgcc cgaagcctga acttctccgc aaacatatac gaatactttt
     3421 ttacgctccg gattttcttc cactgtaccg ctgtccgctt ccgccttttt tctttctgcc
     3481 ttctctgctg gctcttcgct cttttgtgat gtatgatccg acttttcttc ttccgcagaa
     3541 aataaaaatt cttctgtttc aggaacggcc gaatctttgt tcccgcaccc aaacaggaaa
     3601 cttgccgaaa taagaaagaa cactgtcatg acagcgtatt tttttctgta ttttttctga
     3661 ttgtcttgca tattcttctc ctcagctttg ccgggagaat taccccttta tgtcggacat
     3721 taatattgta acgaattttt actaaaatta caatagacaa ttaacgtctt gttacatctc
     3781 tgtcaaccta ttaaggacaa ctttcttttc ctttacaaaa aaaacatata tattacagat
     3841 ttattacttt tatttccatt gaaaaactgc tatgccgaca accgcaagaa ggagaccacc
     3901 tgcttttctc catgagaacg gctcccgatc cattccaaac aatccgaaaa gctggatcag
     3961 ataagcaacc gtaagctgtg cgatcacaat gagaagagct gaacgcgcag gccctaacgc
     4021 ttcgatactt tttataaccg tccatgtgat ccctgcgccg atcacaccgc ctaacagcat
     4081 atatttcggc tgtacttcca taagctgtga aacctgatct cttccggtaa aaaaccacat
     4141 cgcaagacaa accgcaaaag ccgtaaactg tacccagccg tttccgaccc acattcctgt
     4201 tgtcttcgta acctgtgtgt taaatactcc ctgaatactc ataagcgctc cggacaaaag
     4261 agcaattaaa aaaccgatca tatacatacc tccgaactgt ttagatcaaa caatttttct
     4321 aacttccttt agtatgtcct tcgaccggtt tcttattctg tctatttatg taacattttc
     4381 tcttatgaaa acattggaga aatatcttta cggaaacact tttatgcaag tattctgcgc
     4441 atcttctcaa ccatctgatc gatatgctct ttttctacga tcagcggagg aacaaaacgg
     4501 attacgtttc cctgcgcctg tatgataagc agaccttctt caatcgcacg atcattgatc
     4561 tctttgatcg gctttgtgat cacaagaccc tgcatcagac cttttcctct tcgctctgtc
     4621 acacagtcaa attccgcttt aagttcttct aatttttctg taaagtatgg agctatctca
     4681 ttgacatgtc ctacaatatt ttttctctca aaaatggaaa gaactgtctt taccgccgtg
     4741 cacgcaagcg gatttcctcc gtatgtcgcc ccgtgatctc ccggtttaag agaatatccg
     4801 gcaacctctt ccgtcattgc aaacgcgccg accggaatgc cgtttccgat cgctttcgcc
     4861 atcgtaagga tgtccggctt tactccgtac ccctgccatg caaacatcgt acctgttctg
     4921 cccataccgc actgtacttc gtcacagatc ataaggatat cgttttcatc acagagcttt
     4981 ctgatccctc ccataaattc c
//
LOCUS       DBMDXF010000137         4992 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4132_length_4992_cov_2.294623, whole genome shotgun sequence.
ACCESSION   DBMDXF010000137 DBMDXF010000000
VERSION     DBMDXF010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4132_length_4992_cov_2.294623"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            182..1198
                     /locus_tag="ACZPD4_06280"
     CDS             182..1198
                     /locus_tag="ACZPD4_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1378703.1"
                     /translation="MPKGANQKFKLYRLAQIMCEKTDETHYLTMPEIQKELEKYDIIA
                     ERRSLYESLKDLEEFGIEVEGERSGRGYRYHVIGRQFELAELKLLVDAIQSSKFITEK
                     MTNRLIGKLETLVSQHDAADLRRQVFVSGRIKTMNETVYYSVDTIYNAISQNKKIKFQ
                     YYQWNVKKEPELRHGGAYYHISPWGLLWDHENYYLIGYDSRAEQIRHYRVDKMLHILL
                     SEEIREGKEEFQKIDMASYSKKSFGMFGGKEQSVKLLVENSLAGVIVDRFGKDVMMIP
                     ADADHFTVSVDVQVSSQFISWVFALGSQAKIVSPKNVVNQVRKEIEKLAEQYALPIEN
                     RREN"
     gene            1202..3259
                     /locus_tag="ACZPD4_06285"
     CDS             1202..3259
                     /locus_tag="ACZPD4_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1378704.1"
                     /translation="MDEKLKPLFTPWRIGNCEIKNRIVLTSMGGTDLFGWMEKNHFDK
                     MGARFLREAAKNNAGLVLPGCQPVYNPMFGQWLYKNKKMYEDLKEWMPEFHKTGAKLF
                     VQLTAGFGRSFTISEMMEKLYTNKALRVLSKPFMDLDKITATASPSPNRWSDKVPSRE
                     MTREEIHEFVEAFAKSAKLLQDAGVDGVEIHAVHEGYLLDQFTLKYVNHRTDEYGGSF
                     ENRYRFAVEIVQAIKKVCGEDFPVTLRYSVLSKTKGFRKGALPGEQFTEVGRDMEESE
                     KAVKYLQDAGYDMLNCDNGTYDAWYWAHPPVYMPENCNLEDVEHIKQFVRIPVVCAGR
                     MNPKTAAESIAAGRLDGAGFARQFLADQEWVTKMMEDREEEIRPCILCHNGCFNMCHY
                     KGVPNDQELSDSLHLSRCAVNAETMQWDRHFIEKTKNPRRVYIIGGGIGGMEAARVLK
                     LREHDPVIYEKNNVLGGTFIPASAESYKGKLRELLAWYRRQMKELDVEVHLGKEIDSV
                     EKFGDAPVIIATGSVPRVMKNIPGHERMIEACDYLNGRDVGERVVVIGGGLTGSEIAY
                     ELALQGKRPVIVEMKNDLVAQKGVCLANSSYLREWFELHEVPVYLETKLKEVKEHSVI
                     CTDKTGKEIEIPCDSVIGSVGYLPNPLERKGKNIYLVGDCEQVGNLRSVVWGAYETAM
                     KIC"
     gene            complement(3365..4384)
                     /locus_tag="ACZPD4_06290"
     CDS             complement(3365..4384)
                     /locus_tag="ACZPD4_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1378705.1"
                     /translation="MENTELIIPAPSDPLKKHKSHFSKIGLFYFCGAIIISGVQISVI
                     TLIQLIAPQLMVNPNISLIISSLSMYLIAMPLLVIIVRTIPGMVIHKKKMTAGQWIIA
                     FFMCYALMYVTNVIGTFTTAIFGTLKGDLVDNPIQDILTGLSPLTAFFLMVICAPIVE
                     EYVFRKLIIDRTVQYGQATAILLSGLMFALFHGNFNQFVYAFTLGVFWGFIYVKTGRL
                     IYTVALHMTVNFLGSIPGLLLMKSTFFNQLSLLAENNPSAIVGLVMQHPVQFLLICFY
                     MLLLFGLVITGIIFWAINFKKFKCAPGEITIPKGKRFSTIILNVGMILYCLFWIIQII
                     LQLFA"
     gene            4666..>4992
                     /locus_tag="ACZPD4_06295"
     CDS             4666..>4992
                     /locus_tag="ACZPD4_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378706.1"
                     /translation="MKIGETILKLREEKKMSQEEFAQYYHVTRQTISNWEKEKNYPDL
                     QTLVKISDESGVPLDSMLKDNFSMVQEIDKKVRHLKIFKIGTIVVLAIVILVSAYIGI
                     QNGKQDH"
BASE COUNT         1639 a          784 c         1295 g         1274 t
ORIGIN      
        1 acggaaaagg gttatatggg gtatgtagaa ggaacgtgga tgctttttag ctgcgaagcg
       61 gattattatg aattcatgga gtgaaaagcc gtgaagtgag aagtcagaga tggtttccgt
      121 agtggaaaac gcagaaatat gatactatat cattatgact aaagtacggg agcgttgtat
      181 tatgccgaag ggagcaaatc aaaagtttaa gctgtaccgt ctcgcacaga tcatgtgtga
      241 aaagacggat gaaacacatt atctgacaat gccggagatt caaaaagagt tggaaaagta
      301 tgatattatc gcagagagaa gaagtctgta tgaatcgttg aaagatttag aagaattcgg
      361 cattgaagta gaaggtgagc gcagcgggag aggataccgc tatcatgtga taggcagaca
      421 atttgaactg gctgaactga aacttcttgt tgatgcgata cagtcgtcaa aatttattac
      481 agagaagatg acgaaccggt tgataggaaa actggaaact cttgtcagtc agcatgatgc
      541 ggcggatctt cggcggcagg tgtttgtttc agggcggatt aagacgatga atgaaactgt
      601 ttattacagc gtcgatacga tttataatgc aatttcacaa aacaaaaaaa ttaaatttca
      661 atattatcag tggaatgtga aaaaagaacc ggagcttcgt cacggaggag cgtattacca
      721 tatcagcccg tgggggctgc tatgggatca tgagaattat tatctgatcg gatatgattc
      781 ccgggcagag cagatccggc attacagagt cgataaaatg cttcatattt tgctgtcgga
      841 agagatacgg gagggaaagg aggagtttca aaagatcgac atggcgtcgt attcaaaaaa
      901 gagttttggc atgttcgggg gaaaagaaca gtcggtaaaa cttcttgttg aaaattcgct
      961 ggcaggtgtt attgtagatc gtttcgggaa agatgtgatg atgattccgg cggatgccga
     1021 tcatttcact gtgagtgtag acgttcaggt aagcagtcag ttcataagct gggtatttgc
     1081 gcttggcagt caggcaaaaa ttgtcagccc gaagaatgtt gtaaatcagg tgcgcaagga
     1141 gattgagaaa cttgcggagc agtatgcgct tcctatagaa aacagaaggg agaattgaaa
     1201 catggatgag aaactaaaac ctttatttac tccgtggcgt atcggaaact gcgagataaa
     1261 aaaccgtatc gtattgactt ctatgggagg aaccgatctg ttcggatgga tggagaaaaa
     1321 ccattttgac aaaatgggtg cgcgatttct tagggaggct gcaaaaaaca atgcgggtct
     1381 tgtccttccg gggtgtcagc cggtatataa tcctatgttt ggacaatggc tgtacaagaa
     1441 taaaaagatg tatgaagatc tgaaagagtg gatgccggag tttcataaaa caggagccaa
     1501 actttttgtt cagttgacag cgggtttcgg ccggtcgttt acgatcagcg agatgatgga
     1561 aaaattatat acgaataagg cgctgcgcgt tttatcaaag ccgtttatgg atctggataa
     1621 gattactgcg acggcaagcc cgtctccgaa ccgctggtcg gataaagttc cttcaagaga
     1681 gatgacaaga gaagaaattc atgagtttgt ggaagcgttt gcaaagtcgg cgaaactttt
     1741 gcaggatgcg ggagttgacg gagtggaaat acatgcggta catgaaggat atcttcttga
     1801 tcagtttaca ttaaaatatg taaatcatag aacagatgaa tatggaggat cttttgaaaa
     1861 cagatatcgc tttgctgtag agatcgtgca ggcgattaaa aaagtatgtg gtgaagattt
     1921 tcctgtaacg ctaaggtaca gtgttctttc caagacaaaa ggattcagaa aaggcgctct
     1981 tccgggagag cagtttacgg aagtcggaag agatatggaa gaatctgaaa aagcggtgaa
     2041 atatttgcaa gatgcaggtt atgacatgct caactgcgat aatggtacat atgatgcgtg
     2101 gtattgggcg catccgcctg tttatatgcc ggaaaattgt aatctggaag atgtagaaca
     2161 tattaaacag tttgttcgga ttccggttgt atgtgcgggg agaatgaacc cgaaaacagc
     2221 ggcagaatcg atcgccgcag gaagattgga cggagcggga ttcgcgaggc agtttttggc
     2281 agatcaggaa tgggtcacaa agatgatgga agatcgggaa gaagagattc ggccttgtat
     2341 tttatgccat aacggatgtt ttaatatgtg tcactataaa ggagtgccaa acgatcagga
     2401 attaagtgac agcctgcatt tgtcaaggtg tgcggttaat gcggaaacga tgcagtggga
     2461 tcggcatttt atagagaaaa caaagaatcc aagaagagtc tatattatcg gcggaggaat
     2521 cggagggatg gaagctgcca gagtgttaaa gcttcgtgag catgatccgg ttatttatga
     2581 gaagaacaac gttcttggcg gcacgttcat cccggcaagt gcagaatctt ataaggggaa
     2641 attaagagaa cttttggcat ggtacagaag acaaatgaaa gaactggatg tggaggttca
     2701 tttaggtaag gagattgaca gcgttgaaaa gttcggcgat gctccggtaa taattgcaac
     2761 cggttctgtt ccgcgcgtga tgaaaaatat tccgggacat gaacggatga ttgaggcatg
     2821 cgactatctg aacggaaggg atgtaggaga aagagttgta gtgatcggag gaggccttac
     2881 gggaagcgag attgcctatg agcttgcgct tcagggaaag agaccggtta ttgttgagat
     2941 gaaaaatgat cttgtggcac agaaaggagt ttgccttgcc aatagttcct atttgaggga
     3001 atggtttgag ctgcacgaag ttccggtata tctcgaaaca aagttaaaag aagtaaaaga
     3061 acattcggtg atctgtacag ataaaaccgg aaaagagata gagattcctt gtgactctgt
     3121 gatcggatca gtcggttacc tgcctaatcc gctggaaaga aaaggaaaga atatttacct
     3181 ggtcggcgat tgtgaacagg ttggaaattt acggagcgtg gtgtggggcg cttacgagac
     3241 tgcgatgaag atttgctgat atgacgatgc agaatgattg atagaacaag aatagtgaga
     3301 tattagaaaa tgcagctttc tcttgtgagg gtaaagctgc attttaatct gaaaatagat
     3361 ttgtttaagc gaacaactgt aaaataatct gaatgatcca aaacagacaa tacagaatca
     3421 ttccgacatt taagatgatc gttgagaaac gttttccttt cggaattgtg atctctccgg
     3481 gggcacattt gaatttctta aagttgatcg cccaaaatat gatgcctgta atgacaagac
     3541 cgaaaagaag cagcatgtaa aaacaaatca aaagaaactg aaccggatgc tgcataacga
     3601 gtccgacgat ggctgacggg ttattttccg ctaacagaga aagctgattg aaaaatgttg
     3661 atttcattaa aagcagtccc ggaatcgagc ctaaaaagtt gactgtcata tgcagggcaa
     3721 ccgtgtagat caagcgcccg gtttttacat aaatgaatcc ccagaacaca ccgagtgtaa
     3781 atgcgtatac aaattgatta aaattaccgt ggaataacgc aaacatcaat cctgacagaa
     3841 ggatagccgt tgcctgtccg tactgtactg tccggtcgat gatgagtttt cggaaaacat
     3901 attcttcgac gatgggagcg cagatcacca taaggaaaaa tgctgtgagc ggagacagtc
     3961 cggtaagaat gtcctggatc gggttgtcta cgagatctcc ttttagtgtt ccgaagatag
     4021 ctgtcgtaaa tgtgcctatt acattcgtaa catacataag cgcatagcac ataaaaaagg
     4081 cgataatcca ttgccctgcc gtcattttct tcttgtggat gaccatgccg gggatcgtgc
     4141 gtacgatgat gaccaaaagc ggcattgcaa taagatacat cgaaagcgat gagatgatca
     4201 gggaaatatt cggattcacc ataagctggg gtgcgatcaa ctgaatcaat gtaatgacag
     4261 aaatctgcac tcccgaaatt atgatcgctc cacagaaata aaaaagtccg atcttggaaa
     4321 aatgactctt gtgttttttt aacggatcgg acggagccgg gatgataagt tcagtgtttt
     4381 ccatatgaaa taacctcctc ttgcgtaaga ttaaaaatca ataaaaaatt ttacgtctat
     4441 tatcccattt tgcgaagcaa aaatcaataa aaacagaact tatatttgca gccggtgaga
     4501 tgctaaggca gcaaggtgtt aattttagaa atcgatgtta taaggaatat tttcaaaata
     4561 tgaagaggta aaaattattt gcgcccgaaa aaggaggggg taaataaagt ttgctggact
     4621 ttgcagagtg gagagataaa ataaaataaa aaggaggtaa gcggtatgaa aataggagaa
     4681 acaattttaa aattacgaga agaaaaaaag atgtctcaag aagagtttgc acaatattat
     4741 catgtgacga ggcaaactat ttcaaactgg gaaaaggaaa agaattatcc ggatttacaa
     4801 acattagtaa agattagtga cgaatcagga gttccgttgg attctatgct taaggataac
     4861 ttctctatgg tacaagaaat cgataaaaaa gtaagacact taaaaatatt taaaatagga
     4921 acaatagtag tattggcgat agttatttta gtttcagctt atataggaat tcaaaacggc
     4981 aagcaggacc at
//
LOCUS       DBMDXF010000138         4989 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4135_length_4989_cov_2.182897, whole genome shotgun sequence.
ACCESSION   DBMDXF010000138 DBMDXF010000000
VERSION     DBMDXF010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_4989_cov_2.182897"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(56..1456)
                     /locus_tag="ACZPD4_06300"
     CDS             complement(56..1456)
                     /locus_tag="ACZPD4_06300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1378707.1"
                     /translation="MFPEHFLWGGAVSANQCEGAFDEEGKGISIQDVLPKGLKGGMTE
                     EPVKENLKLKGIDFYHRYKEDLKMMAEMGFKVFRTSIAWSRIFPRGDEEKPNQKGIEF
                     YHNLFAECRKYGIEPLVTLSHYETPLYLAEKYDGWRSRKMIDFYKTYVKAVFEEYKDE
                     VKYWLTFNEINSILESPFMSGAIMTPKDELTEEQLYQAAHHELTASAWAVKIGHKINP
                     DFKIGCMILSVPVYPLTPEPDDVLAAKEQEHKSLMFADVHVRGKYPGYALRYFREHGI
                     SLEIEEADREVLKETVDFISLSYYMSACATAHSEKMRRGEGNILGGVPNPMLKSSEWG
                     WQIDPKGLRYVLEIYWERYQKPLFVVENGLGAKDYPIHKEDGSVEIADDYRIEYLRKH
                     IAEMEKAIENGVDVMGYTVWGCIDLVSASTAQMSKRYGMIYVDRNDDGSGTLKRWKKK
                     SFGWYQNVIAKNGREL"
     gene            complement(1482..3437)
                     /locus_tag="ACZPD4_06305"
     CDS             complement(1482..3437)
                     /locus_tag="ACZPD4_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1378708.1"
                     /translation="MNIERERGKCKYPLLFQPLKVNKIVLKNRIISAPLGSNTDKSLS
                     GIGMIIRGTSGSVDDTRMRLTPGTYCFADLFQAAKVREEVSLIRQRGAKAEFELCHCG
                     QFAVVEEGDYAIGPVSFVRNDGTEVRAMDEKMMDEIADKFAKSACDAKEYGFDGVLLH
                     FGHGWLPAQFLSPYFNKRKDEYGGSFENRIKFPMMIVDRVRQAVGRDYMLDMRISGEE
                     HIEGGMEIDEVAEFIKRIEDKIDMVHISCGVEIDNRTKIYMSPTAYAPHKINAKMAKL
                     VKEKVKIPVAVVGGIMTPEEAEEILEDGCADAVVIGRQLIADPWWVKKAWEGRSEDIV
                     PCIRCMNCYNPYQYKTEEERRKHVGLNTVPCCSVNPRYLHEDRVPNELPEASVKKKTV
                     VVGGGPAGMQAALTAARRGHQVILLEREGELGGQLICSEYDEVKQDLKRLKDYLILQV
                     QKAEVEVRLNVTATPEMVKELSPDSLILALGAYPIQLNLPGTEKNDHVFTCIEAYKYQ
                     EKIGENVVVIGGGSIGCELGVELSRKGKQVTILEIEKVLNRTLNDIMKTALKTEMEKC
                     RTLTVLTETRCVSIEEGFVTAASKDGEEFEIKADTIIFAAGMKARTDEANTFFGIAQD
                     TNIIGDANRVGTVWNAMEDGYYISAGL"
     gene            complement(3440..4798)
                     /locus_tag="ACZPD4_06310"
     CDS             complement(3440..4798)
                     /locus_tag="ACZPD4_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1378709.1"
                     /translation="MDLKRTAREVLENVGGEENIISIMSCFTRVRVELKDKTKVDEKK
                     LKEADGCQGVNWVGNTVQIIFGGRCNDVYDELEKIVHITETEANGEKSIVKKQSVWAV
                     VIDYIANSIQPVVPILIASGFLQSVLAMLNYLDIDTTTYTYQVLNSIGQAGYYFLPIF
                     LAFAAAKKLRINPYLGALVGAVMVYPAIIEAGSAGGTASFLGVPVTLVSYASSITPIL
                     ISMPVVMWINKLAKKISPKVLSSLLIPIITMILSIPVVLIVTGPVATWIGNGLCGVVS
                     FIFTKMSVVGGLLIGGAAPYLVLTGVHNGIALPITLSELASQGFSYFFPLLAYGNIAV
                     GGAALGVWFKTKNNRLKTTAMSSCLMAVVGITEPALFGVLLPAKKPLIALGVMGAVCS
                     AVSLMFGVKCTALSMCGLGGLPAFFGDTFVIWCILMAVSFVGAFLITILIGFKDIPEE
                     EA"
BASE COUNT         1343 a         1373 c          709 g         1564 t
ORIGIN      
        1 gaaacaacag atgtcataca gttcggacat ctgttgtttt tctcaatttt gacttttata
       61 attcccttcc atttttagct attacatttt gataccagcc aaaacttttt ttcttccaac
      121 gctttaacgt tccgcttccg tcatcatttc tatctacata aatcatccca taccgtttac
      181 tcatttgggc cgtagacgcg ctcacaagat caatacatcc ccaaaccgta tatcccataa
      241 catcgacgcc gttttcaatc gccttttcca tttccgcaat atgttttcgt aaatactcta
      301 tccggtaatc gtcggcaatt tccacgcttc cgtcctcttt atgaatcgga taatctttcg
      361 ctccaagtcc gttttcgaca acaaacaacg gtttttgata tctttcccag tatatttcca
      421 atacatatct caatcctttc ggatctatct gccatcccca ttcgctcgat ttcaacatcg
      481 gattaggaac gcctcccaat atatttcctt cacctcttcg catcttttcc gaatgtgcag
      541 tcgcgcacgc gctcatataa taactcaatg aaataaaatc taccgtctct ttcaaaactt
      601 cccgatccgc ttcttctatc tctaaagaaa ttccatgttc tctaaaatat ctcagcgcat
      661 atcccggata ttttccccga acatgaacgt ctgcaaacat taagctttta tgctcctgtt
      721 cttttgccgc caggacatca tccggttccg gagtcagcgg atatacaggc acgcttaaaa
      781 tcatacaccc gattttaaaa tccggattaa ttttatgacc gatcttcact gcccatgcgc
      841 ttgcagtcag ttcatgatgc gccgcctgat aaagctgctc ttccgtcagt tcatctttcg
      901 gagtcatgat cgctccactc ataaaaggag actccagtat tgagttgatt tcattaaatg
      961 taagccagta tttcacttca tctttatatt cttcaaatac tgcctttaca tatgttttat
     1021 aaaaatcaat catctttctg ctgcgccatc catcgtattt ttccgctaaa tacaacggag
     1081 tttcgtaatg cgacaatgtc acaagcggtt cgattccgta ttttctgcat tctgcaaaca
     1141 agttgtgata aaattctatt cccttctgat tcggtttttc ttcatctcca cgcggaaaaa
     1201 tccgactcca tgcaatcgat gtccgaaaaa ctttaaatcc catctcagcc atcattttta
     1261 aatcttcttt ataccgatga taaaaatcaa ttccttttaa ctttaaattc tctttcaccg
     1321 gctcttctgt cattccacct ttcaaccctt tcggaagtac atcttgtatg gagattcctt
     1381 ttccctcttc atcaaacgca ccttcgcatt gatttgcgga aaccgctccg ccccaaagaa
     1441 aatgttcagg aaacatatcc acatcgcccc tttcgccaaa gtcataatcc cgcgctaata
     1501 taatatccgt cctccatcgc attccatacc gttcctaccc gatttgcatc gccgattata
     1561 ttcgtgtcct gcgcgattcc gaaaaatgta ttcgcctcat cggttctagc cttcatccct
     1621 gccgcaaaaa taattgtatc tgcttttatt tcaaattctt ccccatcctt agatgccgca
     1681 gttacgaatc cctcctctat cgaaacacat cgagtctctg tcagcacggt taatgttctg
     1741 catttttcca tttctgtttt taaagcagtt ttcataatat cattcaaagt tcggttcaac
     1801 actttttcaa tttcaagaat cgtcacctgt tttccttttc tgctcaattc aacccccaac
     1861 tcacatccga tagatccgcc gcctataacg accacatttt caccaatctt ttcctgatat
     1921 ttataagctt ctatgcatgt aaaaacatga tcattctttt ctgtgcccgg aagatttaac
     1981 tgtatcggat aagctccaag tgcaaggatc agactgtcag gactcaattc cttaaccatt
     2041 tccggtgtag cggttacatt taatcttact tctacttccg ccttttgaac ttgaaggatc
     2101 aaataatctt tcaatctctt taaatcctgc tttacctcat catactctga gcagatcaac
     2161 tgtccgccca attccccttc tcgttctaaa agaatcacct gatgtcctct tcgtgccgcc
     2221 gtcaacgcag cctgcattcc ggcaggacct ccgccgacta ctactgtctt tttctttaca
     2281 gacgcttccg gcagttcatt cgggacgcga tcttcatgca aatatctcgg attcaccgag
     2341 cagcacggaa ctgtattgag cccgacatgc tttcttcgtt cttcctctgt tttatattga
     2401 tatggattat aacagttcat gcatctgata cacggaacaa tatcttccga tcggccttcc
     2461 catgcttttt tcacccacca tggatcggcg atcaactgac gtcctatgac aacagcatcg
     2521 gcacatccgt cctcaagtat ttcttccgcc tcttccggag tcataatccc tcccactacc
     2581 gctacaggta tcttcacttt ttctttcaca agttttgcca ttttcgcatt gattttatgc
     2641 ggtgcatatg ccgtaggcga catgtagatt tttgtacgat tatctatttc cactccgcag
     2701 gaaatatgaa ccatatctat cttatcttca atccttttta taaattccgc aacttcatca
     2761 atctccatgc cgccttctat atgctcttct ccacttatcc gcatatccaa catataatct
     2821 cgtccaaccg cctgccgcac cctgtctacg atcatcatcg ggaattttat tctattttca
     2881 aagctccccc catactcatc ttttctttta ttaaaatacg gagaaagaaa ttgtgccgga
     2941 agccatccgt gtccgaaatg aagcaatacc ccatcaaacc catactcttt tgcgtcacat
     3001 gccgactttg caaatttatc tgcgatttca tccatcattt tttcatccat agcccgcacc
     3061 tctgttccat cgtttcttac gaaggatacc ggccctatgg cataatcacc ttcttccaca
     3121 accgcaaact gaccgcagtg acacaattca aactccgctt tcgcaccgcg ctgtctgatc
     3181 aatgaaactt cttctctgac tttagccgcc tggaataaat ctgcaaaaca atacgttccc
     3241 ggagttaaac gcatacgcgt atcatccacc gatccgctcg ttcctctgat aatcatccct
     3301 atcccactta atgatttatc tgtattactt cccaatggag ccgaaataat cctgtttttc
     3361 aaaacaattt tgttcacttt caacggttga aacaaaagtg gatatttaca ttttcccctt
     3421 tccctttcaa tattcatttt tacgcctcct cttccggtat atctttaaac ccgatcaata
     3481 tcgtaatcaa aaatgctccg acaaaagaaa ccgccatcaa aatacaccaa ataacaaatg
     3541 tatctccgaa gaatgccggc aggccgccaa gtccacacat acttagtgcc gtacatttta
     3601 ctccgaacat caaagaaacc gccgagcaga ccgctcccat aactcctaat gcgatgagtg
     3661 gttttttcgc agggagcaac accccaaaca atgccggttc tgtaatcccc actacagcca
     3721 ttaagcaaga cgacattgct gtcgttttca aacgattatt tttcgtctta aaccaaacgc
     3781 caagagcagc tcctccgacc gctatgttgc catatgccaa caatggaaaa aaataggaaa
     3841 aaccctgact cgcaagttcc gacaaagtta tcggcaaagc aattccatta tgaacgccgg
     3901 tcaaaacaag atacggcgca gcccctccga taagcagccc tcctacaacc gacatctttg
     3961 taaatataaa cgaaacaact ccgcataatc cgtttccaat ccacgttgcc acaggacctg
     4021 ttacaatcaa aacaaccggt attgataaaa tcattgtaat aatcggaatc aaaagacttg
     4081 acaatacttt gggactaatt ttttttgcta gtttgttgat ccacatgaca accggcatag
     4141 aaatcaaaat aggagtaatt gatgacgcat aagatacaag cgtaaccggc actcccaaaa
     4201 atgatgccgt accccctgcg cttcctgctt cgattatcgc aggataaacc attaccgctc
     4261 ctaccaatgc cccaagataa ggattgatcc ttaatttttt ggcagctgca aacgcaagga
     4321 atatcgggag gaaatagtat cccgcctgtc ctatagaatt caatacctga tatgtatatg
     4381 tcgtcgtgtc aatatcaagg taattcagca tcgccaacac actctgtaaa aatccagacg
     4441 ctatcaagat cggaacaaca ggctgtatcg agtttgctat atagtcaatc actaccgccc
     4501 atacagactg cttcttaaca atactctttt caccgttcgc ttccgtctcc gtaatatgaa
     4561 caattttctc taattcatca tagacatcat tacatcgtcc gccgaaaata atctgcactg
     4621 tatttccgac ccaattcacg ccctgacatc catccgcttc ttttagcttt ttctcatcaa
     4681 cctttgtttt atcttttagt tccacacgaa cacgagtaaa acaactcata atactgatga
     4741 tgttctcctc tcctcccaca ttttccaaaa cttctcttgc cgttcttttc agatccatat
     4801 gccttctcct ttcttttaac tttttatttt tatataagca aacagaccca aaggcagagg
     4861 catattccac ctttgagtct ggcctaatct aatagtgacc acctcatcac tccatgtttt
     4921 attatttatt acatttccat tattctttat tggcaacaca gtttctcaaa tgcaatacaa
     4981 gataatttt
//
LOCUS       DBMDXF010000139         4977 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4146_length_4977_cov_2.145816, whole genome shotgun sequence.
ACCESSION   DBMDXF010000139 DBMDXF010000000
VERSION     DBMDXF010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4146_length_4977_cov_2.145816"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="ACZPD4_06315"
     CDS             <1..721
                     /locus_tag="ACZPD4_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1378710.1"
                     /translation="KHGGKIMFQMKNRWKNILVGCLTVFGILIPSLSCRAENVGSIQI
                     EYHGRTQENEEVVFANADFSVFFVGKMEDGKWTLAGDFGNAGVSLEGIESSEKNEQAK
                     QLYNYAVRQSIQGNALKTDENGIAMIGGLEQGLYLIAQTKVWTDEKQGSYQASPYLIS
                     IPEEIDGSYIWDVVTKPKSEWITEAPQHPEMPDKNTETEKTEGAKTGDTSSAALSLLL
                     LIFSSGAFIILCRKRRIYRKD"
     gene            834..1373
                     /gene="rbr"
                     /locus_tag="ACZPD4_06320"
     CDS             834..1373
                     /gene="rbr"
                     /locus_tag="ACZPD4_06320"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1378711.1"
                     /translation="MGSKYAGTETEKNLRNAFSGESEARNKYTYFASVAKKEGYEQIA
                     ALFRDTADNEKEHAKMWFKELNGIGDTADNLKAAAEGENYEWTEMYAGFAETAEREGF
                     PELAQKFRLVAEIEKHHEERYRALLKNVETASVFEKSEVKVWECRNCGHIVVGTAAPE
                     ICPTCAHPRSYFEIHAENY"
     gene            1528..3072
                     /locus_tag="ACZPD4_06325"
     CDS             1528..3072
                     /locus_tag="ACZPD4_06325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1378712.1"
                     /translation="MREYRNRICSAVLAAVCVIGTALIPSRAVHAEDVVDPIAVQKQA
                     AYDAVPETNGLENWPQGPHVYANSAIVMEMNSGAILYGKKINDKHYPASITKLLTALV
                     ALENADPDDEVYFSEDSVSFLEYGDASIGMRPGEILSMNDALYGMLLASANEVSYAIA
                     ESVGKKMGGGFETFIQKMNERCEELGCTGSHWTNANGLHDENHYTTAHDMALIGSAVY
                     QFDKFREVTQTLNYTIPPTNLVNESRTFQQNHKMLWVGAHYSYDYCTGGKTGYTDQAK
                     TTLVTMADNADMQLVAVVLEDQGDVYVDTRAMFDYVYANFSKVFLNEHDKPDGVRKFK
                     TEDAYVVLPKGIDVSSLEHEITITDRQNAAGKLTYLYKGQNVGTVEVKLTADYIKEET
                     GYNIEPEMKSVGKKSTDKEEKTEMPIYVKLLIAVVLLVIILLAVLFSLLKYRQVKRRR
                     ARQLARKRKKALERRRSNTEQTGENAFRRQSSAGRRKNTQIRNRQVDDRRSGSYRRRD
                     REGRYR"
     gene            3215..>4977
                     /locus_tag="ACZPD4_06330"
     CDS             3215..>4977
                     /locus_tag="ACZPD4_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR1378713.1"
                     /translation="MKKKWNKVTAALLMLVMVVTTVLGAAPALSVKAEPVTMGHLKSG
                     SGNANGHFGSATPEAFVLSEKADYTNQAYSLKLRINSDVDKTRLRIVTKYKDDNNWAF
                     LMYDVGSWKWQYKKDGNEAWQNDTLALPRLEKGQLAEIKLSYKDNGLEIDVNGTKATV
                     TEANFVALKDVQGKVGVGGATFGDAYTDVYFNDVVVGETKEDLNTWKLYKDNLAGQEW
                     DPAAQYEETVPAGRKWITIRTGSQNGGGHSYAGATGPALLLDKDRTIKPADVFSLSFT
                     PINSGNFGFFYSYVNDNDFLYIGYDSSSKWYWQNGSGSYGSLNGDFETPENGKEMTFS
                     VTMSREALTVTVNGISGTTNNQNAGNWLETNAGKGHPGVMVKTANQSLKFADAKVNET
                     ACMEDTWEFAKDRDGQSQSSEYINLATVSGTVTAKDGGAALKGATVRFGSKSAKTDDN
                     GAYQIEDVEVGTYTAAASCPGYEAITQEVEVQDAAEGENVFNFTLSEKTPIDLKNYKS
                     IESDYMKVYVGPNFPVVARYEVKGKDDVYFRGNESDLAKVNTVVINGKEITPEVKAKI
                     EGAKASYEMTLKYEGEDEETK"
BASE COUNT         1685 a          820 c         1328 g         1144 t
ORIGIN      
        1 aaaacacgga gggaagatca tgtttcaaat gaaaaataga tggaaaaata ttcttgtagg
       61 ttgtttgact gtattcggaa tactgattcc aagtttatcc tgcagggcgg aaaatgtagg
      121 ctctatccag attgaatatc atggaaggac acaggaaaat gaagaggttg tatttgcgaa
      181 tgcggatttt tctgtttttt ttgtcggaaa gatggaggat ggaaagtgga cgcttgcagg
      241 agatttcggt aatgcgggag tttctctgga aggaatcgaa tcttctgaaa aaaacgaaca
      301 ggcaaagcag ttatacaact atgcggttcg gcaaagtata cagggaaacg ctttgaaaac
      361 ggacgagaat ggaatcgcaa tgatcggcgg attagaacag ggattgtatt tgattgccca
      421 gacaaaggtg tggaccgatg agaagcaggg cagttatcag gcatcgccgt atctgatcag
      481 tattccggaa gaaatagacg gcagctatat atgggatgtt gtaacgaaac caaaaagcga
      541 gtggatcacg gaagcgccgc agcatcctga aatgccggat aaaaatacgg aaacggagaa
      601 gacggaagga gcgaaaacag gagatacaag ctcggcggct ttgtcattgc ttttactgat
      661 attcagttcg ggagcgttta taattctatg tagaaaaaga agaatataca ggaaggactg
      721 acataatatc cggcatttga atgttaattg tttaatataa gctgactgtg ttataatagg
      781 atataaaaaa ggaaaggctg tttcttttga gaggagcaga aagggtgaat aacatgggaa
      841 gtaaatatgc aggaacagag acggaaaaaa acttaagaaa cgctttctcg ggagagtcgg
      901 aggcgcgcaa taaatacacg tattttgcgt ctgtggcgaa aaaagaaggg tatgaacaga
      961 ttgccgcgtt gtttcgggat acggcggata acgagaaaga acatgcaaaa atgtggttta
     1021 aagaattgaa cgggatcgga gatacggcag ataacttgaa agcggcggca gaaggcgaga
     1081 actatgaatg gacagaaatg tatgccggtt ttgcggaaac tgccgagaga gaaggatttc
     1141 cggaacttgc tcaaaaattc cgtcttgtgg cggagatcga aaagcaccac gaagagagat
     1201 acagagcgct gctgaaaaat gtagagacgg catcagtatt tgaaaaaagt gaagtgaaag
     1261 tgtgggaatg cagaaactgc ggacatattg tagtaggaac tgcggcgccg gagatctgtc
     1321 cgacctgtgc gcatccgaga agttattttg aaatacatgc agagaactat taaaatgctg
     1381 aaacccatcc atgaaaattg acatcccttc tggaagcgga tgaaaatact tcggaaaatc
     1441 taaaacttgt aaaacaaaaa gaatatcgtt ataataaatt attagagaaa aataatctga
     1501 agggttatat cgatgtggag ggttataatg agggaatata gaaaccgtat atgttcggca
     1561 gtgctggcag cagtctgtgt gatcggcaca gcattgattc cgtcaagagc cgtacacgca
     1621 gaagatgtag ttgacccgat cgcggtgcag aaacaggcgg catatgatgc cgttcctgag
     1681 acaaacggtt tggaaaactg gccgcaggga ccgcatgtat atgcaaattc ggcgattgtt
     1741 atggagatga acagcggggc gattttatac gggaaaaaaa taaacgataa gcattatccg
     1801 gcaagcatta caaaactgtt gacagcgctt gtggcgttgg aaaatgcgga tccggatgac
     1861 gaagtttatt tttccgaaga cagcgtttcg tttctggaat acggagatgc gagtatcggg
     1921 atgagaccgg gagaaatact cagtatgaac gatgcgctgt acggtatgct gcttgcatct
     1981 gccaatgagg tgtcttatgc aatcgcagaa agtgtcggca aaaagatggg cggaggtttt
     2041 gagacgttta tccagaaaat gaatgaacgt tgcgaggaac ttggatgtac gggatctcat
     2101 tggacgaatg caaatggtct gcatgatgaa aaccattata cgacggcgca tgacatggcg
     2161 ttgatcggat cggcagtcta tcagtttgac aagtttcggg aagtaactca gactttaaat
     2221 tatacgattc cgcctacaaa ccttgtgaat gaatccagaa cgttccaaca gaatcacaag
     2281 atgctttggg taggggcaca ttattcttat gattattgta cgggcggcaa gaccggatat
     2341 acggatcagg caaaaacgac tcttgtgact atggcagata atgcagatat gcagcttgtt
     2401 gcggttgtct tggaagatca gggagatgtg tatgtagata caagggcgat gtttgattat
     2461 gtatatgcga atttttccaa agtgtttttg aatgaacacg acaagcccga tggcgtcagg
     2521 aagtttaaaa cggaagatgc atatgttgtt ctgccgaaag gaattgacgt ttcatctctg
     2581 gagcatgaga ttacgattac tgacaggcaa aatgccgccg gaaagctgac ctatttatac
     2641 aaaggtcaaa atgtcggaac tgtagaagtg aaactgacgg cggattatat taaggaagaa
     2701 acagggtata atattgagcc ggaaatgaaa tccgtcggga agaaaagtac ggataaagaa
     2761 gaaaaaacag agatgcctat atatgtaaag ctgttaatag cggtagttct tttggtaatc
     2821 attttgttgg cagttctttt cagtctgctg aaatatcggc aagtgaaacg aaggagagca
     2881 agacagcttg caagaaaacg caaaaaggca ttagagcgcc ggcggtcaaa tacagagcag
     2941 acgggagaaa atgctttccg acgacagagc agtgccggca ggagaaaaaa tacccaaata
     3001 agaaaccgtc aggtagacga cagaagaagc ggaagttatc gcaggcgaga cagagaaggg
     3061 cggtacagat aaataaaaca aacaaaagat caacattaaa gataaaaagt gtaaatactg
     3121 aaaaaagtga aagagttatg atatataaga taggatgctc tttctatgtc ctgaaggagc
     3181 taatattaaa atttaaagaa aggaaaagag gataatgaaa aagaagtgga acaaagtaac
     3241 tgcagcttta cttatgctcg tgatggtagt gacgacagtg ctcggcgctg ctccggcatt
     3301 atctgtcaag gcagagccgg tcacgatggg acatctgaaa tccggaagcg gtaatgcaaa
     3361 cggacatttc ggaagcgcta cacctgaagc atttgttttg tcagaaaaag ctgattatac
     3421 aaatcaggca tatagtttga aacttcgcat taacagcgac gtagacaaaa caaggcttcg
     3481 tattgtcaca aaatataaag atgacaataa ctgggctttt ctgatgtatg atgtcggaag
     3541 ctggaagtgg cagtacaaaa aggacggaaa cgaggcttgg cagaatgata cgcttgctct
     3601 tccgagactt gaaaaaggtc agttggcaga aattaagtta agctataaag ataatggcct
     3661 tgaaattgac gtcaatggaa caaaggcaac tgtaacggag gcgaactttg tcgctcttaa
     3721 agacgttcag ggtaaagtcg gagtcggagg cgcgacattc ggagatgcat acacagacgt
     3781 ttattttaat gatgttgttg taggagagac aaaagaggat ctcaatacat ggaaattgta
     3841 taaagataat cttgcaggtc aggaatggga tccggcggca cagtacgaag aaacagttcc
     3901 ggcaggacgt aaatggatca cgatccggac aggctctcag aacggcggag gacatagcta
     3961 tgcgggagca acaggaccgg cgcttctgct tgacaaagat cgcacgatca agccggcaga
     4021 cgtatttagt ctgtcattta caccgatcaa cagcggaaac ttcggtttct tctattcata
     4081 tgtaaatgat aatgatttcc tgtatatcgg atatgattca tcttccaagt ggtactggca
     4141 gaacggctca ggctcatacg gaagtttgaa cggagatttc gagactccgg aaaacggaaa
     4201 agagatgacg ttctctgtta cgatgagcag ggaagctctg acagttacag tcaacggaat
     4261 cagcggaaca acgaacaatc agaacgcagg aaactggctg gaaacaaacg caggaaaagg
     4321 tcatccgggc gtgatggtga aaacggcaaa ccagtctttg aaatttgcag atgcgaaagt
     4381 aaatgagact gcatgtatgg aagatacatg ggaatttgca aaagacagag acggacagtc
     4441 acagtcttca gaatatatca atctcgcaac tgtttccgga actgtaacgg caaaagacgg
     4501 aggagcggca ttaaaaggcg caacggttcg ttttggttca aaatcagcaa aaacagatga
     4561 taacggagct tatcagatcg aagatgttga agtgggaact tatacagctg cggcaagttg
     4621 tccgggatac gaagcaataa cacaagaggt tgaagttcag gatgcggcgg aaggagaaaa
     4681 tgtatttaac tttacacttt ccgagaaaac gccgatcgac cttaagaatt ataaatccat
     4741 cgagtctgat tacatgaaag tgtatgtagg accgaacttc ccggttgttg caagatatga
     4801 agtaaaagga aaagatgacg tttacttccg tggaaacgaa tcagatcttg caaaggtgaa
     4861 tacagttgtg atcaacggca aggagatcac tcctgaagtg aaggctaaga ttgaaggggc
     4921 gaaagcttct tatgaaatga ccctcaaata tgaaggtgaa gatgaagaaa caaaaat
//
LOCUS       DBMDXF010000140         4941 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4185_length_4941_cov_2.223760, whole genome shotgun sequence.
ACCESSION   DBMDXF010000140 DBMDXF010000000
VERSION     DBMDXF010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4185_length_4941_cov_2.223760"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            36..>4941
                     /locus_tag="ACZPD4_06335"
     CDS             36..>4941
                     /locus_tag="ACZPD4_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1378714.1"
                     /translation="MNATIYVNGKKLGTHPYGYTPLSFDITDNVKFGKENVIAVKVDH
                     QTPSSRFYSGSGIYRDVDFVVTDTVHVDKNGTKIETPDLKEHVDGNNVAVKVKTTVVN
                     ESERDASVKVKHTIYPKNGTAEQAVATFESQEAVAVEKGKSKDVSADFTASGVQLWST
                     TTPNLYTVKTEVLMDGATVDTYETDYGFRYFAFDKNNGFTLNGQKMKLQGVCMHHDQG
                     ALGSVANDRSTERQVEILKMMGCNSIRVTHNPASDELIDACNKHGILVIDEAFDGWVA
                     PKNGNSNDYAKWFNKEIESDNEIMGAADNMTWAQFDLTAMIERGQNDPAIIMWSLGNE
                     MWEGTGGYSAAYKTAQDNLVKWAQAADTTRPVTTGDNKLKGEEEGSITLANGLQEASG
                     IHGMNYASGAEYDKIHKKYSEWPLYGSETASAVNSRGIYKGMNSQHNYGNHDLTSYDT
                     SAVGWGATASSAWYEVIKRDFLAGEYVWTGFDYIGEPTPWNDPYGSLGQGGTWPLPKN
                     SYFGIVDTAGLPKDSYYFYQSQWNDSVNTLHILPAWNEDVVYKKEGNKVPVVVYSDAK
                     KVELFFTPANGGEKRSLGEKEFAQKTTTAGYTYQMYEGEGKSSKEHENLYMTWMVPYE
                     AGTITAKAWDKDGQEITENIQGRTSVTTTGAAAKLQVKADRETIKANGEDLSYLTVNV
                     TDENGNIVPNAENKVTFEVSGDGVLAGIDNGRPIDHQSYRDDNRKAFSGQLVGIVRST
                     KSAGTITVKVKADGMEDQTVQIKTEASSDGTTEEKAISSVEMSKNYYVKVGNRPQLPA
                     ELDVILNDKSEAKGKVTWEDVAEDQVNQSGTFSVTGTITVEGVDKAETVSVNVNMIDT
                     VAALLNYSTTTSVGVEPILPTSRPAVMEDGTILTASFPVTWEKPKDGYNTAGIVQVKG
                     TADVFGESMDVTASVRVQEAEVTVGANIAKDALTLKQDLTVTSDTLEAIRDGSREVSS
                     NTSGGANTTLWSNYDNSNQNRDDKDAEITFEYATKMNFNQIKIFFRQDSYSATYPDAK
                     TTQIFVSDTGAADTWTLVDTTETIAQQDNNGVKEYKYDFAPTSGVFVKIRVVNSNATG
                     KPAGFTCTAIAEAELYKAETADFATNTTAELQSLTVDGKAAPAEALTAGSWNVSAKEV
                     KEIAAVGKDNAAVTILPAKNNAVKILIESEDHKTQNTFVINLGVADPADDSKDYTGVI
                     TPSAGSTQPNNGTDIDKAFDKKNDTFWHSSWNPSTTNENLYVTMELAEAQKIDAIRYL
                     PKGGNLTGGDQNGRVKTYTVEVSMTGADDSWTKVEITPSTQEWANGTDWKIAQFVQPV
                     EAKFIRFTGVETYGDGGQENKFMSAAEIRVKLAEDEPVVPEPKPTELVIQNQPTKTTY
                     TEGEKFDPTGLKVGVKYDNGEVKDVAEYNAETAGQFTFDPALNTALTTNNTKVTVTYK
                     GKTADIIIQVNPKEEEPKVTGIEVKTLPKTKYTEGDKFDPTGLVLTVKYDKGEDKEVA
                     YGDATKADFTFSPSLDTALKTSDEKVTVTYAGKTAEIGIEVKADTPVEPEKPTVDKIA
                     VKKVPAKTTYKAGETFDPSGLVLTVTMSDKTTKEVAYGDETAKDFVFNPTLDTALTEG
                     MNKVDVTYAGKTAEIGIEVKADTPV"
BASE COUNT         1743 a          864 c         1234 g         1100 t
ORIGIN      
        1 gcaaacgtat cagtatcgac ttcggcggcg tgtacatgaa tgcaactatt tatgtaaacg
       61 gtaaaaagct cggtacacat ccgtatggat acacaccatt gtctttcgat attacagata
      121 atgtgaagtt cggaaaagaa aacgtgattg ccgttaaagt agatcaccag acaccgtcaa
      181 gccgttttta ctcaggaagc ggtatttaca gagacgtaga ctttgttgta accgatacag
      241 ttcatgtaga taagaacgga acaaagattg agacaccgga tctgaaagag catgtagatg
      301 gaaacaatgt tgcggtgaaa gtaaaaacaa ctgttgtaaa tgaatccgag agggatgcat
      361 ctgtaaaagt aaaacatacg atctatccga aaaacggaac ggcagagcag gcagttgcta
      421 cgtttgagtc acaagaggca gttgctgtag aaaaaggtaa gtcaaaagac gtatctgcag
      481 attttacagc atcaggcgta cagctttgga gtacaacaac tccaaatctc tatactgtga
      541 aaacagaagt ccttatggac ggtgctacag ttgatacata cgagacagat tatggtttcc
      601 gctactttgc atttgataaa aacaatggat tcactctgaa tggtcagaag atgaaacttc
      661 agggagtatg tatgcatcat gatcagggcg cgcttggttc ggttgcaaat gaccgctcta
      721 cagagcgtca ggtagagatc ctgaaaatga tgggatgtaa ctctatccgt gtgacacata
      781 atccggcgtc agacgaattg atcgatgcat gtaataagca tggaatctta gtgatcgacg
      841 aggcatttga cggatgggta gctccgaaaa acggcaattc aaacgattat gcaaaatggt
      901 ttaataaaga aattgaatct gataatgaaa tcatgggcgc tgcggacaat atgacatggg
      961 cgcagtttga tcttacagca atgatcgagc gtggacagaa tgatccggcg attattatgt
     1021 ggtcgctcgg taacgagatg tgggaaggaa caggcggata tagtgctgct tataagactg
     1081 ctcaggacaa tcttgtgaaa tgggcacagg cggcggatac tacacgaccg gtaacaaccg
     1141 gagataataa gctaaaaggc gaagaggaag gctcaattac tctcgctaat ggcttacagg
     1201 aagcgtcggg aatccatgga atgaattatg caagcggcgc ggagtatgac aaaattcaca
     1261 aaaaatattc agaatggccg ttatatggtt cggaaacggc atcagcagtt aacagccgag
     1321 gaatttataa aggcatgaat agtcagcata attatggaaa tcatgatttg acgtcttatg
     1381 atacatcggc agtaggctgg ggagctacgg caagcagcgc ttggtatgaa gtaattaaaa
     1441 gagatttcct tgccggtgaa tatgtatgga caggatttga ctatatcgga gaaccgactc
     1501 cgtggaatga tccatatgga agtttaggac aaggtggcac atggccgctt ccgaaaaatt
     1561 cttattttgg aattgttgat acggcaggac ttccgaagga cagctattac ttctaccaga
     1621 gtcagtggaa tgacagtgtg aatacactgc atattcttcc ggcatggaat gaagatgttg
     1681 tgtacaaaaa agaaggaaat aaagttccgg tagttgtata cagcgatgca aaaaaagtag
     1741 aattgttctt cactccggca aatggcggtg agaagagatc gcttggcgaa aaggaatttg
     1801 cacaaaaaac gacgactgca ggatatacat atcagatgta tgaaggcgaa ggaaagagca
     1861 gtaaagaaca tgagaatttg tacatgacat ggatggttcc atatgaagcc ggaacaatta
     1921 ctgcaaaagc gtgggataaa gacggtcaag aaataacaga aaatatccaa ggacgcacat
     1981 cggtaactac gacaggagcg gcggcgaaac ttcaagtaaa agccgacaga gaaacgatta
     2041 aagcaaacgg tgaagatctt tcttatctta cagtgaatgt tacagatgaa aacggaaata
     2101 ttgtgccgaa tgcggaaaat aaggtgacat ttgaagtgag cggcgatggt gtgcttgcag
     2161 gtatagataa cggaagacct atagatcatc agtcctacag agatgacaat agaaaagcat
     2221 tcagcggaca gcttgtaggt atcgttcgtt ccacaaaatc ggcaggaaca atcactgtga
     2281 aagtcaaagc ggatggaatg gaagatcaga cggtgcagat taaaacagag gcatcatcag
     2341 atggaacaac tgaagaaaaa gcaatcagca gcgttgagat gtccaaaaac tactatgtaa
     2401 aagtgggtaa tcgtccacag cttccggcag aactggatgt tattcttaat gataagtcag
     2461 aagcaaaagg aaaggtaaca tgggaggacg ttgcagaaga tcaggtcaat cagtcaggta
     2521 cattcagcgt gacaggaaca attacagttg aaggtgtcga taaagcagaa acggtttccg
     2581 ttaatgtaaa tatgattgat acagtagcag cgttgttaaa ttattcaaca acaacaagtg
     2641 ttggagtaga accgattctt ccgacatcaa gacctgctgt catggaagat ggaacaatac
     2701 ttacggcatc attcccggtg acatgggaaa aaccaaaaga cggttataat acagccggaa
     2761 ttgtacaagt aaaaggaacg gctgatgtgt tcggggaatc aatggatgtt acggcatctg
     2821 tgagagttca ggaagcggaa gttacagttg gtgctaatat cgcaaaagat gcactgactt
     2881 tgaaacagga cctcacagtt acttcagata cacttgaagc aatccgcgac ggcagccgag
     2941 aagtttccag caatacaagc ggtggagcaa atacgacgct ttggagtaat tatgataatt
     3001 ctaatcaaaa cagagatgat aaagatgcag agattacgtt tgaatatgca acaaagatga
     3061 actttaatca gatcaagatt ttctttagac aggatagtta ctcagcaaca tatccggatg
     3121 cgaagacaac acagattttc gtatcagata caggtgcagc agatacgtgg acattagttg
     3181 atactacaga gactattgca caacaagata ataacggagt aaaagagtat aaatatgatt
     3241 ttgcgccgac aagcggtgtg tttgttaaga ttcgtgtggt gaattctaat gcaaccggta
     3301 aaccggcagg atttacatgt acagcgattg ctgaggcgga attgtataaa gcagaaaccg
     3361 cagattttgc aacaaacaca acagcggaac tgcagagcct gactgtagat ggtaaagcgg
     3421 ctccggcaga agcgcttaca gcaggatcat ggaatgtttc ggcaaaagaa gtgaaagaaa
     3481 tcgcagcagt aggaaaagac aatgcggctg taacaatact tccggcaaag aataatgctg
     3541 taaaaatatt gatcgaatca gaagatcata aaacacagaa tacatttgtg atcaacctcg
     3601 gcgtggcaga tccggcagat gacagcaagg attacacagg agttatcact ccgtcggcgg
     3661 gaagcactca gcctaataac ggaactgata ttgacaaagc atttgataaa aagaatgata
     3721 cgttctggca ttcaagttgg aatccgtcaa caacaaacga aaacctttat gtaactatgg
     3781 aacttgcaga ggcacagaag attgatgcaa tccgttattt gccgaagggt ggtaatctta
     3841 caggaggaga tcagaacggt cgtgtgaaga catatactgt agaagtaagc atgacaggtg
     3901 ctgatgattc atggacaaag gtagagatta ctccttcaac acaagagtgg gcaaacggaa
     3961 cagattggaa aatcgctcag tttgtacagc cggttgaggc gaagtttatc cgctttacag
     4021 gtgttgaaac ttatggtgac ggaggtcagg agaacaagtt tatgagcgcg gctgagattc
     4081 gtgtgaaatt agcagaggac gaaccggtag taccggagcc aaagccgaca gaacttgtaa
     4141 tccagaatca gccgacgaag acaacttata cagaaggtga aaaattcgat ccgacaggac
     4201 ttaaagtcgg agttaaatat gataatggtg aagtgaaaga tgtagcagaa tataacgctg
     4261 aaacagcagg acagttcaca tttgatccgg cattgaatac agcgcttact acaaataata
     4321 cgaaggtaac tgttacatat aaaggtaaaa cagcagacat tatcattcag gttaatccaa
     4381 aagaggaaga gccgaaagta acaggaattg aagttaagac attaccgaag acaaaatata
     4441 cagaaggcga taaattcgat ccgacaggac ttgtgcttac agtgaaatat gacaaaggcg
     4501 aagataaaga agtagcttac ggcgatgcaa caaaagcaga cttcacattc agcccaagcc
     4561 ttgacacagc actgaaaaca agtgatgaga aagtaacagt aacatatgca ggcaagacgg
     4621 cagaaatcgg tattgaagtg aaagctgaca caccggtaga gccggaaaaa ccgacggtag
     4681 acaaaattgc cgttaagaaa gtgccagcta agacaactta taaagcagga gagacattcg
     4741 atccgagcgg acttgttctt acagtaacga tgtcagacaa gacaacaaaa gaagttgcat
     4801 acggagacga aacagcaaaa gattttgtat ttaacccaac acttgataca gcacttacag
     4861 aaggtatgaa caaagttgat gtaacatatg caggcaagac ggcagaaatc ggtattgaag
     4921 tgaaagctga cacaccggta g
//
LOCUS       DBMDXF010000141         4912 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4199_length_4912_cov_1.833922, whole genome shotgun sequence.
ACCESSION   DBMDXF010000141 DBMDXF010000000
VERSION     DBMDXF010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_4912_cov_1.833922"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            25..930
                     /gene="modA"
                     /locus_tag="ACZPD4_06340"
     CDS             25..930
                     /gene="modA"
                     /locus_tag="ACZPD4_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1378715.1"
                     /translation="MKKRVIAVIAVLALSVAMFGCKAGSDTGNGKKQREESAGDMGTE
                     GKTTAEEETEIQVFIAASLNTVMTELAKEYQKDHPNVKIIYNADSSGTLLTQIEEGYE
                     CDLFFSAAQKQMDVLEADGFLVDGTRSDVVNNQVVVITLKDSKTKVKGLANLQDAESI
                     ALAGGSVPVGKYTRQALVNMGIIHTSNDPDSITAQEISEAFGGVEISEQDNVSKVLMA
                     VAEGSCEVGTTYYSDIYGYEDDIKILEKVSSDLTGDVIYPIAQIVNKEADKKQTEAAE
                     DFMDFLLSDEAAKVFESYYFDTDIK"
     gene            999..1703
                     /gene="modB"
                     /locus_tag="ACZPD4_06345"
     CDS             999..1703
                     /gene="modB"
                     /locus_tag="ACZPD4_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1378716.1"
                     /translation="MDEVTEILKNLDWSPLYISLKTGVAATVISFFLGIFAAGKAVKA
                     SPGKKAVLDGILTLPMVMPPTVAGFFLLLLFSRRRPVGAFLFEEFGFKVVQTWIGCVI
                     AAIVISFPLMYRNARAAMEQIDVNLVYAGRTLGMSETKIFWKIVIPTAGPGIASGTIL
                     TFARAMGEYGATSMLAGNIQGKTGTIAQKIAMVIQDGDYVTAGIWVAVVVLIAFVIIF
                     LMNFISGRKMKNIGRW"
     gene            1707..2792
                     /locus_tag="ACZPD4_06350"
     CDS             1707..2792
                     /locus_tag="ACZPD4_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1378717.1"
                     /translation="MAIDMLIRKKWDKFSLDINFKSQSKRIGILGASGCGKSMTLKSI
                     AGIETPDSGHIKIDGNILFDKETKTNLKTQKRKVGYLFQNYALFPTMTVEQNIAAGLK
                     GNKQDIHKRVKEMIQKFGLAGLEKHYPKQLSGGQQQRTALARIMAYEPEVILLDEPFS
                     ALDLYLKDRLEQEVLQMLESYMGTVIMVSHNRDEIYRFSEELFVMDKGRIVASGKTEE
                     LFQNPKNKTAAILTGCKNFSSIIYIDDHTVEAKEWGICLKTKRKVPKDAKWIGYRAHD
                     FIPVWGTRGENMLKFQLESSARLPFEQNYYIAAENRIGNSTEKNGEKDKTMICWFVQR
                     EKMEEIEKKGFPDYLWIDEGKILFLNE"
     gene            complement(3068..3382)
                     /locus_tag="ACZPD4_06355"
     CDS             complement(3068..3382)
                     /locus_tag="ACZPD4_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378718.1"
                     /translation="MIDKRLVPYSGLKKEPFSGSHNGMRYMMRSDNGRDSTTFTVFVY
                     PEPWCFEQTPEDQIESHTFDLSEEGLDLAITWLWERFEAERDRWTQASQEMMHTVKKH
                     TL"
     gene            complement(3434..3988)
                     /locus_tag="ACZPD4_06360"
     CDS             complement(3434..3988)
                     /locus_tag="ACZPD4_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1378719.1"
                     /translation="MVFEYILKENGIDPEKDLTIIQNIDFGSTAPAFADGRGDFTIEF
                     EPGATSLESEGKGHVIASLGKDSGYVPYTAFSAKKSYLKENPDVIQRFTNALQKGMDY
                     VRSHSPSETASVIKPQFPETTLEMITTIVTRYYEQDTWKSNLIFEESSFDLLQDILES
                     AGELEKRAPYKDLITTEFAENAAK"
     gene            complement(3978..>4912)
                     /locus_tag="ACZPD4_06365"
     CDS             complement(3978..>4912)
                     /locus_tag="ACZPD4_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1378720.1"
                     /translation="KYYICTTYNTKGRRYCEKAHLIEENDLMEDVLNYIKLCREALCE
                     VISTYDMRDFDAEKKTLAEKRQDIQEEIQNRRNQLKILLTQKIKDLSIASENEDLIHE
                     TYNSMQKDLLAQIHGMEIQEKELNETTIETPDVKDKLRNALDVVDKIIAGGTLDRRDI
                     ELLIERIDVDKEGMPEITLKYGLSDLINYSPADEMNRRENTVIALVMKLIIEDERGYT
                     SAKYLSEHITALGFKKTKQSILPYINLMKELGILEDTDNPLKPYNIVKTKKEIMILMH
                     QYLPDLPAQMTSKQLSDNYLHYYGGNRRYAGNGI"
BASE COUNT         1493 a          847 c         1092 g         1480 t
ORIGIN      
        1 gtaaataaaa gaaaaggaaa taatatgaaa aaaagagtca ttgcagtgat tgctgttctc
       61 gccctttcag ttgccatgtt tggctgcaaa gcgggaagcg ataccggaaa cggcaaaaag
      121 caaagagaag aatctgccgg ggatatgggt acagaaggaa agactacggc agaggaggaa
      181 acagaaatac aggtttttat tgcggcgagt ctgaatacag ttatgacgga acttgcaaag
      241 gagtatcaaa aggatcatcc gaatgtgaag atcatataca acgcggatag ttccggaact
      301 cttctgacgc agattgaaga aggatatgaa tgcgatcttt tcttttcggc agctcagaaa
      361 cagatggatg tgcttgaagc agacggattt cttgtagacg gaacgagaag tgatgtggtg
      421 aataatcagg ttgtcgttat aacgcttaaa gacagcaaaa caaaagtcaa agggctggca
      481 aaccttcagg atgcagagag cattgcgctg gcaggaggaa gcgttcctgt cggaaaatat
      541 acaagacagg ctcttgtgaa tatgggaatt atccatactt caaacgatcc ggattctatt
      601 acggcacagg aaatttccga agcgtttgga ggagttgaga tcagcgagca ggataacgta
      661 agcaaagtgc tgatggcagt tgcggaaggt tcatgcgaag tgggaacgac ttattattct
      721 gatatatacg gatacgaaga cgatattaag attttagaaa aagtaagttc tgatctgacc
      781 ggagatgtga tctatccgat cgctcagatt gtgaataaag aagctgataa aaagcagacc
      841 gaagcggcgg aagattttat ggattttctt ttatctgatg aagcggcaaa agtgtttgag
      901 tcatattatt ttgatacgga tataaaatag tgtgcattca gatcaggtta aggcaggaga
      961 taatgcattc gtacgcaaat agatgtggag tataaataat ggatgaagta acagaaatat
     1021 tgaaaaatct ggattggagc cctctgtata tttctttgaa aacaggtgtg gcagctacgg
     1081 tgatttcgtt ttttctcggg atatttgcgg ctggaaaagc agtaaaagct tccccgggga
     1141 aaaaagcggt gctagatggg attttgacgc ttcctatggt gatgcctcct acggtggcag
     1201 gctttttttt actgttgttg tttagcagga gaagaccggt cggagcattt ttatttgaag
     1261 agtttggttt taaagtcgtg cagacatgga ttggatgtgt gatcgccgcg attgtaattt
     1321 cgtttccgct gatgtatcga aacgcaagag cggcaatgga gcaaattgat gtgaatctcg
     1381 tgtatgcagg ccgtacactc ggcatgtcag agacgaaaat tttttggaaa atcgttatac
     1441 cgactgccgg tccgggaatt gcgtcaggaa caatcctgac atttgcaaga gcgatgggag
     1501 aatatggggc gacgtctatg ctggcaggaa atatacaagg aaagacgggg acgatcgctc
     1561 agaagattgc gatggtcatt caggacggag attatgttac ggcaggaatt tgggttgcgg
     1621 ttgtagttct gatcgctttt gtcattattt ttcttatgaa tttcatttcc ggacgaaaga
     1681 tgaaaaatat cgggaggtgg taaaagatgg cgattgatat gctgattcgg aaaaaatggg
     1741 ataaattcag tttggatatc aattttaaaa gtcagtcaaa acgaatcggt attttagggg
     1801 catcaggctg cggtaaaagt atgacattaa aaagtattgc gggaatagag actcctgata
     1861 gtggtcacat taagatagac ggaaatatat tatttgataa agaaacgaaa acaaatctaa
     1921 aaacacagaa acgtaaagtt ggatacttgt ttcaaaatta cgcgctattt ccgacgatga
     1981 cagtagagca aaatatagcg gccgggctaa aaggaaataa gcaggatata cataagcggg
     2041 tcaaagagat gattcaaaag tttggtctgg caggtctgga aaaacattat ccaaagcagc
     2101 tttccggagg acagcagcaa agaacggcgc ttgctcggat tatggcttat gaaccggaag
     2161 ttattttatt agacgagccc ttttccgctc ttgatctgta tttaaaagac cgattggagc
     2221 aagaagtgct tcagatgtta gagtcatata tgggaactgt gattatggta tcacataatc
     2281 gagatgaaat ttaccgtttt agtgaagagc ttttcgttat ggataagggg aggattgtcg
     2341 catccggcaa aaccgaggag ctgtttcaaa atccgaaaaa taagactgcc gccatactta
     2401 caggatgtaa aaatttctca agtattatct atatagatga tcatacagta gaagcaaagg
     2461 agtggggaat ttgcttaaag acaaagagaa aagtaccaaa agatgcgaaa tggattgggt
     2521 atcgggcaca tgattttatt cctgtgtggg gaacaagggg agaaaatatg cttaaatttc
     2581 aacttgagag cagcgcccgc cttccgtttg agcagaacta ttatattgcg gcagagaata
     2641 gaataggaaa cagtacagaa aaaaacggtg aaaaagacaa aactatgatt tgttggtttg
     2701 ttcaaaggga aaaaatggaa gaaatagaga aaaaaggatt tcctgactat ttgtggatcg
     2761 atgaggggaa aatattgttt ttgaatgaat aacagtcgat ccttaacaga taataagata
     2821 ccgctgtttt aatatttttc atatttaggc gaagtccgac gactgtacat accgactgaa
     2881 tatacagtcg tcgtctcctt ttatggaaac aacagaaact gaattttgat catgctttct
     2941 gttgttatgg acgatgggga atacactgtt ttgtgtttcc tatcgttttc atatatttaa
     3001 gataacacgt aattgtgaat gtgatgtgac aatatatgga agaaaaatta aaatttctaa
     3061 aaagaaacta aagtgtatgc tttttgacag tatgcatcat ttcctgtgat gcctgtgtcc
     3121 aacggtcacg ttcggcttca aaccgctccc acaaccatgt gatcgccaga tcaagccctt
     3181 cttcggaaag gtcaaaagta tgactttcaa tttgatcttc cggcgtttgt tcaaaacacc
     3241 atggttcagg atagacaaaa acggtgaatg ttgtgctgtc tctgccattg tctgaacgca
     3301 tcatgtaccg cattccgtta tggctgcctg aaaaaggttc tttttttaaa ccgctgtaag
     3361 gaacaagtct tttgtcaatc ataaaaatct cccgttgttt tagtgtttgt cacatcaaat
     3421 ataagctatt atatcatttg gcggcatttt ctgcaaattc agtcgttata agatctttat
     3481 acggagctct tttttcaagc tccccggcgc tttctaggat atcctgaagc agatcaaagc
     3541 tgctttcttc aaaaatcaga ttgcttttcc acgtgtcttg ttcatagtaa cgtgttacga
     3601 tggtcgtgat catctcaagt gttgtttccg gaaactgagg cttgattaca gatgcggttt
     3661 cggaaggact gtgagagcgg acataatcca ttcccttttg aagcgcgttt gtaaaacgct
     3721 ggatcacatc agggttttct ttcagataac tttttttggc gctgaatgcg gtgtaaggaa
     3781 catagccact gtctttgcca agcgatgcaa taacatgacc ttttccttcg gattcaagag
     3841 aagtcgctcc gggctcaaat tcaatcgtaa aatctccgcg gccatccgca aaagccggag
     3901 ctgtagatcc gaaatcaata ttttggataa ttgtaagatc tttttcaggg tcaattccgt
     3961 tctcttttaa aatatattca aataccattt ccggcatacc gcctgttacc accataataa
     4021 tgtaaataat tgtccgataa ttgtttagaa gtcatttgag ccggtaaatc cggtagatat
     4081 tgatgcatca atatcataat ctcttttttt gttttcacaa tattgtaagg tttcagcgga
     4141 ttatccgtat cttccaaaat tcctaattct ttcatcaaat tgatataagg taaaatactt
     4201 tgtttcgttt tcttaaatcc caatgctgtg atatgttctg acaagtattt tgcagatgta
     4261 tatccacgtt catcttctat aatcagcttc attacaagag cgattacagt attttcccgg
     4321 cgattcatct catcagcagg actgtaattg attaaatcag acaaaccata ttttaaagta
     4381 atttccggca ttccttcttt atctacgtca attctctcaa tcaacaattc aatatctctt
     4441 cggtcaagag ttccacctgc aatgatttta tcaactacat ccaatgcatt tctcaatttg
     4501 tcctttacat ccggtgtttc tatagtagtt tcgttcagtt ctttttcctg tatctccata
     4561 ccatgaatct gcgccagtag atctttttgc atagaattat aggtttcatg gatcaaatcc
     4621 tcattttcag atgctatgga taaatctttt attttttgtg ttaaaagtat cttcagttga
     4681 tttcttcggt tttgaatttc ttcttgaatg tcctgtcgtt tttctgccag tgttttcttt
     4741 tctgcatcaa agtccctcat atcatatgtg gaaattactt cacaaagcgc ttctctgcac
     4801 agtttaatat aattcagcac atcttccatc aaatcatttt cctcaatcag atgcgctttc
     4861 tcgcaatacc gcctgccttt cgtattatag gtagtacaaa tataatattt tc
//
LOCUS       DBMDXF010000142         4882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4228_length_4882_cov_1.535453, whole genome shotgun sequence.
ACCESSION   DBMDXF010000142 DBMDXF010000000
VERSION     DBMDXF010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_4882_cov_1.535453"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="ACZPD4_06370"
     CDS             complement(<1..390)
                     /locus_tag="ACZPD4_06370"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HIR1378721.1"
                     /translation="MYELLKKDGMAKRGRFHTVHGTIETPVFMNVGTAAAIKGAVSTD
                     DLRQIKTQVELSNTYHLHVRPGDEIVKKMGGLHRFMNWDKPILTDSGGFQVFSLASLR
                     KIKEEGVHFHSHIDGRKIFMGPEESMQI"
     gene            complement(446..1855)
                     /locus_tag="ACZPD4_06375"
     CDS             complement(446..1855)
                     /locus_tag="ACZPD4_06375"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1378722.1"
                     /translation="MKENKGKRSKKAWIIGGSIIGGLLVIYLGISAFFISHFYINTTI
                     NGKDFSGKTESDVVAYMKDQVEGYSLKIIEADNKTDEINGEAISMTYKKDDSVKDALK
                     NQNPLLWIRALFSKSSAEVTINVDYNEDALSKAIGSIQAVKAEQTEPVSAYPKFDGES
                     FKVEPEVYGSEVDMEILTKKIKEYITNFEPELNLLNEKCYKVPKYTTESKEVQKACDD
                     MNKYCQASITYPMKENVVVDKALISTWVSADADMNVTFNEEAVRAWMRDFGKTYDTVG
                     TTRTITSPTGKTVEVSGGTYGWSIDEEAETQNLIASIKNGEVVTREPAYEKTAASHAA
                     QDWGTTYLEVDLSAQHMWYIVNGAIALETDVVTGLPDAKHATPAGVYSILYTEPDSKL
                     IGEKDPETGKPIYETYVRYWMPFTYQGHGFHDADWQTAFGGSRYQSYGSHGCVNMPVD
                     QAGALFNMLSAGTPVVLHY"
     gene            2300..3406
                     /gene="aroC"
                     /locus_tag="ACZPD4_06380"
     CDS             2300..3406
                     /gene="aroC"
                     /locus_tag="ACZPD4_06380"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1378723.1"
                     /translation="MSGSTFGKIFRITTWGESHGSGIGVVIDGCPSGISLNEEDIQIF
                     LDRRKPGQSKFTTRRSEEDTVEILSGIFEGKTTGTPISLLVRNKDQRSRDYGNIVSMF
                     RPGHADYPFTEKYGIRDYRGGGRSSGRETIGRVAAGAIASKILNELGISLCAYTKAIG
                     PYVINETEYNYSEISQNSLYMPNNNIAEQAGQYITSLMKETNSCGGVIECIADGLPVG
                     LGEPVFDKLDALLAQAVMSIGAVKGVEIGDGFQAASSVGSKNNDPFYVENGEVHKKTN
                     HSGGTLGGMSDGSRLIVRAAVKPTSSIAIPQETINTAHENTEIVIHGRHDPVIVPRAV
                     VVVEAMTAITLTDLLLQNMTAKMDHLKQIYTKEI"
     gene            3416..>4253
                     /locus_tag="ACZPD4_06385"
     CDS             3416..>4253
                     /locus_tag="ACZPD4_06385"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378724.1"
                     /translation="MSNRLIDIVDITKSYGDSVILDQLNLYIRENEFLTLLGPSGCGK
                     TTLLRILGGFETPDSGKIIFDGKNINDIPPNRRQLNTVFQKYALFSHMTIADNIAFGL
                     KIKGKSKAYIKDKIRYALKLVNLEGFEDRSPDSLSGGQQQRIAIARAIVNEPKVLLLD
                     EPLGALDLKLRQDMQYELIRLKNELGITFVYVTHDQEEALTMSDTIVVMNQGYIQQIG
                     TPEDIYNEPQNAFVADFIGDSNILPATMVEDKVVKILGAVWNCVDVGFGHNKPVDAVI
                     RPE"
     assembly_gap    4254..4263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4264..4500
                     /locus_tag="ACZPD4_06390"
     CDS             <4264..4500
                     /locus_tag="ACZPD4_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1378725.1"
                     /translation="DIDLVAPGEGVINGVVTNLIFKGVHYEMEVLANNYELLVHSTDM
                     FPVGTEVGVKVDPFDIQIMKKPESEDEEAVPIEE"
     gene            4490..>4882
                     /locus_tag="ACZPD4_06395"
     CDS             4490..>4882
                     /locus_tag="ACZPD4_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1378726.1"
                     /translation="MKNRKILAGPYLFWAMSFIIIPLLMILYYGLTDKNGSFTLNNLS
                     QMTTPENLKALGLALLLSFISTLICLVLAYPLAMILAEKNMNQTSFIVLIFILPMWMN
                     FLLRTLAWQTLLEKNGVINSILNFLHLPE"
BASE COUNT         1325 a         1129 c          931 g         1487 t
ORIGIN      
        1 gatctgcata ctttcctccg gacccataaa gattttacgt ccgtctatat gagagtgaaa
       61 atgaactcct tcttctttaa tcttgcgcaa tgatgcaagt gaaaacactt gaaatccgcc
      121 tgagtccgtg agaatcggct tatcccaatt cataaagcga tgcagaccgc ccattttttt
      181 cacgatttca tcccccggtc tgacatgaag gtgatatgta tttgacagtt caacctgcgt
      241 tttaatctga cggagatcat ctgttgatac ggctccctta attgccgccg ccgttcctac
      301 gttcataaaa accggcgtct cgatcgttcc gtgtaccgta tggaacctcc ctctttttgc
      361 cataccgtct ttttttagca attcgtacat gtatataacc tctgattttc tgtttttcaa
      421 taaattctaa gtcttatcat ttacattaat aatgaagaac aaccggcgtt cccgcgctaa
      481 gcatattaaa taaagctccc gcctgatcta ccggcatatt aacacagccg tgagaaccat
      541 atgactgata tctcgaaccg ccgaatgctg tctgccagtc tgcatcatga aagccatgtc
      601 cttgataagt aaacggcatc cagtagcgga catacgtttc gtaaatcggt ttccctgttt
      661 ccggatcttt ttcaccgatt aattttgaat ccggttctgt ataaagaatc gaatatacac
      721 ctgccggagt cgcatgcttc gcatcgggaa ggcctgtaac aacatctgtc tccagtgcga
      781 tcgccccgtt tacaatatac cacatatgct gtgcggaaag gtccacctca aggtacgtcg
      841 ttccccagtc ttgtgccgca tgagatgccg ctgtcttttc atatgcaggt tctcttgtca
      901 caacttctcc gtttttaata cttgcgatca aattctgcgt ttctgcctct tcgtcgatcg
      961 accagccgta agtacctcct gatacttcca ctgtctttcc tgtcggagaa gtgatcgttc
     1021 tcgttgttcc gactgtatca taagtttttc cgaaatctct catccatgcc cggacagctt
     1081 cttcattgaa tgttacattc atatcagcat ccgccgaaac ccatgtcgaa ataagagctt
     1141 tgtctacaac tacattttct ttcatcggat aggtaatact tgcctgacaa tatttattca
     1201 tatcgtcaca cgctttttga acttcttttg attccgttgt atatttaggt actttatagc
     1261 atttttcatt cagaagattt aattccggtt caaaattggt aatatactct ttgatctttt
     1321 tagtcaaaat ttccatatct acctcacttc catacacttc cggctctact ttgaatgatt
     1381 caccgtcaaa tttaggatat gcagataccg gttccgtctg ctctgctttc actgcctgaa
     1441 tcgacccgat tgctttactc agtgcatcct cattataatc aacgttgatc gtcacttctg
     1501 cgctggattt tgaaaaaagc gctcgtatcc acagaagagg attctgattt ttcaatgcgt
     1561 cttttacact atcatctttt ttatacgtca ttgaaattgc ctctccatta atctcgtccg
     1621 tcttattatc agcttctata atcttcaaag aatacccttc cacttggtct ttcatatatg
     1681 ctacgacatc cgattctgtt ttccccgaaa agtcttttcc gttaattgtt gtattaatat
     1741 agaaatgact gataaaaaac gccgaaatac caaggtatat cacgagcagt ccgcctatga
     1801 tgcttcctcc aatgatccac gcttttttac tccgctttcc tttattttct ttcatctttt
     1861 tctgccgacg tctttttccc cttcttcgga gcactttctt ttttttcttt tttccctctt
     1921 catcttcatc ttcatacagc tcatcgtcat caatatcatc atcgtcaaag ctgtcgtccg
     1981 cgctgaagtt gtcactctcc gaaaaatctt cctcatcttc aaattcttca aacacttctt
     2041 catctaaaag ccgtacgtct tcttgccggc gtaaggattt atttttcttt tcactcatcc
     2101 gttaatcatc tctctccctt tttcaatatc acggcaacat tatattacga ttgtaaaaaa
     2161 aagtacagta tattgtttta ttttaagaaa aattaagacc ttgcttgtaa ctgctccttt
     2221 cttcctatat aatgactttt gtaattctgc ttttcgacag aactttacat ataacagaat
     2281 tttgaaagga gatcatttta tgagtggctc aacttttggt aaaatatttc gcatcacgac
     2341 ttggggagaa tctcacggat ccggcatcgg cgtcgtgatc gacggatgcc cttccggaat
     2401 ttctctaaat gaagaagata ttcagatatt tcttgaccgc cgaaaacccg gtcagagtaa
     2461 atttacaacg agacgcagcg aggaagatac tgtcgagatt ctctcgggaa tctttgaagg
     2521 caaaacgacc ggaacaccga tttctcttct cgtacgtaat aaagaccaac gttcccgaga
     2581 ttatggaaac attgtttcta tgtttcgtcc ggggcatgcg gattatcctt ttacggaaaa
     2641 atacggcatc cgcgattaca gaggaggcgg gcgttcttcc ggtcgggaaa caatcggccg
     2701 tgtcgcagca ggcgcgattg cctctaaaat cttaaacgaa ctcggaattt ctctttgtgc
     2761 ttacacgaag gcaatcggtc cttacgtaat aaatgaaaca gaatataact actccgagat
     2821 cagccagaac agtctttata tgccaaacaa caatatcgcc gagcaggctg gacagtacat
     2881 tacttcttta atgaaagaaa ctaattcctg cggcggagtt atcgaatgta ttgcagacgg
     2941 actgcctgtc ggcttaggag aaccggtctt tgacaagctg gacgcgcttc tcgcacaagc
     3001 tgtcatgtcg atcggcgctg ttaaaggcgt agaaatcgga gatgggtttc aggcagcctc
     3061 ttctgtcggc tcaaaaaata acgatccttt ctatgttgaa aatggagaag tacataagaa
     3121 gacaaatcat tccggaggta cgcttggagg catgagcgac ggctcccgac tgatcgtccg
     3181 ggcagcagta aaaccgacat cctctatcgc aataccgcag gagacgataa atacagcgca
     3241 tgagaatacc gaaatcgtca tccacggaag acacgaccct gtaatcgttc cccgcgcagt
     3301 cgttgttgta gaagctatga ccgcgatcac gcttaccgat cttcttctgc agaatatgac
     3361 agcgaagatg gatcatttaa aacaaatcta tacaaaagaa atttaggagg ccgctatgtc
     3421 taaccgattg atcgatattg tcgatattac aaaatcttat ggggattctg tcattttaga
     3481 ccaactgaat ctttatatac gggaaaacga atttttgact ttgctcggac caagtgggtg
     3541 cgggaaaaca acactgctga gaattttagg tggttttgaa acacctgatt ccggaaagat
     3601 catttttgat ggaaaaaata ttaatgacat tccgccaaac cgccggcagc taaacaccgt
     3661 tttccaaaaa tatgctcttt tttcccacat gacaatcgct gacaacattg cgttcggctt
     3721 aaagatcaaa gggaaatcaa aagcttacat taaagataaa atccgctatg ctttaaaact
     3781 tgtcaatttg gaaggttttg aagaccggtc accggactca ttaagcggcg gacaacaaca
     3841 gcgtatagcc attgcgcggg cgatcgtaaa tgaacccaaa gtgctccttt tagacgaacc
     3901 gctcggagct cttgatctga agcttcgcca agatatgcag tacgaactga tccgtctgaa
     3961 aaacgaactc ggcatcacct ttgtttacgt tactcacgat caggaagaag cgcttacaat
     4021 gtctgatacg atcgttgtta tgaatcaagg ctatatccaa cagatcggta cgccggagga
     4081 catttacaat gaaccgcaaa atgcttttgt cgcagatttt atcggtgaca gtaatattct
     4141 gcctgctacg atggtagaag ataaagtcgt aaagatactc ggagctgtct ggaactgtgt
     4201 agacgtcgga tttggtcata ataaaccggt agatgctgta atccgacctg aggnnnnnnn
     4261 nnngacattg atcttgttgc tcctggagaa ggagttatca acggagttgt cacaaatctg
     4321 atcttcaaag gggttcacta tgaaatggaa gttctcgcaa acaattatga gcttcttgtt
     4381 catagcaccg atatgttccc ggttggaaca gaagtcggag taaaagtcga tccttttgat
     4441 atccagatca tgaaaaaacc ggaatccgaa gatgaggagg ctgttccgat tgaagaatag
     4501 aaaaatactc gcaggtccat atttattctg ggcaatgtct tttatcatta ttcctctgct
     4561 catgatcttg tactatggac tcacagacaa aaacgggagt tttactctga ataatctttc
     4621 ccagatgaca actcccgaaa atttaaaagc gctcggactg gctcttttac tgtcgtttat
     4681 cagcacttta atctgcttag tcctcgccta tccactggct atgattcttg ccgagaaaaa
     4741 tatgaatcag acaagtttta tcgtactgat attcattctt ccgatgtgga tgaattttct
     4801 tcttcggaca ttagcgtggc agacactttt ggaaaaaaac ggagtcatta acagcatact
     4861 taatttcctc catcttccgg ag
//
LOCUS       DBMDXF010000143         4881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4230_length_4881_cov_1.481172, whole genome shotgun sequence.
ACCESSION   DBMDXF010000143 DBMDXF010000000
VERSION     DBMDXF010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_4881_cov_1.481172"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="ACZPD4_06400"
     CDS             <1..553
                     /locus_tag="ACZPD4_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR1378727.1"
                     /translation="LDPMDIWRLKKGVGKEFTALMEQEIELGIVDGMILPNVRMTPEG
                     DVCPFLDVYGRCSIHAYRPGICRLFPLGRYYEENGIKYFIQIHECARKERGKIKIKKW
                     LGIANLKAYEDYIWKWHGFLKDCENAIFGLDDKNRNILTLYVLRSFFEAPYRADDENG
                     FYEEFSVKMEEVRKKLDGLKDFS"
     gene            688..1137
                     /locus_tag="ACZPD4_06405"
     CDS             688..1137
                     /locus_tag="ACZPD4_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HIR1378728.1"
                     /translation="MAWAIFSMLFLAIGGVVYGAAALIWFALTILANWLMFMKAGEAG
                     WKSIIPVYNTYTAYKIAWTPNMFWIYFIFTVLESIFSSISGGDFWSFSGFLGGLCGLV
                     TIVLSVFYCVNLAKSFGKGLGFTIGLIFLEPIFVMMIAFGSARYQKY"
     gene            complement(1308..2798)
                     /gene="galT"
                     /locus_tag="ACZPD4_06410"
     CDS             complement(1308..2798)
                     /gene="galT"
                     /locus_tag="ACZPD4_06410"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1378729.1"
                     /translation="MLYESIKKLVQYGIDTGLTPECERVYTTNLLLELFHEDNYEDIE
                     CTSDNRDLEEILKDLLDEAVKRGIIEDSITYRDLFDTKIMNCLMPRPSQIQKEFQEKY
                     NISPEEATSYYYKLNQDSDYIRRYRIKKDRKWTVETKYGTLDITINLSKPEKDPKAIA
                     AAGKAKSASYPKCQLCMENEGYAGRSNHPARENHRIIPITIQGRSWGFQYSPYVYYNE
                     HCIVFNGEHVPMKIDRNTFAKLFDFIKLFPHYFLGSNADLPIVGGSILSHDHFQGGNY
                     TFAMAKAPIIENFTVPGYEDVEAGIVKWPLSVIRLRGENTEQIIDLADHILSKWRTYT
                     DEEAFIFAETDGVLHNTITPIARKKDGMYELDLTLRNNITTEEHPLGVYHPHEELHHI
                     KKENIGLIEVMGLAVLPARLKGEMELLKEYIIEKKDIRSNDQIAKHADWTDAFLSEYD
                     NVTEENIEQILEQEVGKVFCKVLEDAGVYKCTDEGIKAFKRFISTL"
     gene            3019..3861
                     /locus_tag="ACZPD4_06415"
     CDS             3019..3861
                     /locus_tag="ACZPD4_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1378730.1"
                     /translation="MEASYKHSYKTGENLLNSLSVYNVGYQKCDPEYQWGPGVRDHYC
                     IHHILSGSGCYCTGTVSKHLEEGDTFILYPGVELRYQADADKPWEYAWAGFMGADAAS
                     LIRNTEFSRETPYILKGRIPTEKIRDGLERIYNAKGNTYEAAVAMTGEMYSLLAVFMH
                     YAEHEEKEKAIQLTYAEKAESYIETNYSYPITVEEIASYVGISRSHLFRSFQNYMNRS
                     PKEYLTEYRIKQACRLLRETDLSVSAIAYSVGFENNLYFSKAFRKQKGESPSEYRKSR
                     VRKI"
     gene            3951..4646
                     /locus_tag="ACZPD4_06420"
     CDS             3951..4646
                     /locus_tag="ACZPD4_06420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1378731.1"
                     /translation="MERNRKIWMQILVLAAVLRFFPFDFQTAVSTASEDVAAECAGGE
                     EQKPKIALTFDDGPNIKYTPMLLDGLKERGIKATFFLIGANTEQEESKKIVKRMYEEG
                     HLIGNHTYRHVDLSKISGREAEKEIKTTDEAIKKITGHETEFIRPPFGAMPEGEEDPD
                     KIYVKWTVDSLDWVTKDTEKIVEKVTADTKEGDVILMHDCYGESVEAALQIVDIMEKQ
                     GYEFVTADELIIN"
     gene            4676..>4881
                     /locus_tag="ACZPD4_06425"
     CDS             4676..>4881
                     /locus_tag="ACZPD4_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR1378732.1"
                     /translation="MINEYSRTELLIGSENMEKLKKASVAVFGVGGVGSHCIEALARC
                     GIGRLILIDNDDVSLTNINRQSIA"
BASE COUNT         1437 a          773 c         1219 g         1452 t
ORIGIN      
        1 attagatcct atggatatat ggcggcttaa aaaaggggtc ggaaaagaat ttacggcttt
       61 gatggaacag gaaattgagc tggggattgt agacggtatg atattgccga acgtgcggat
      121 gacgccggaa ggagatgtct gtccgttttt agatgtgtat ggcagatgca gcatacatgc
      181 atatagaccg ggtatctgcc gtctgtttcc tcttggcaga tattatgaag aaaacggaat
      241 aaaatatttt atccagatcc atgaatgtgc gagaaaggaa cgtggtaaga taaaaataaa
      301 aaaatggctc ggaattgcaa acttgaaagc gtatgaggac tatatatgga agtggcatgg
      361 atttctgaaa gattgtgaaa atgcgatctt cgggctggat gataaaaaca gaaatatatt
      421 gactctatat gtgctgagga gcttttttga ggcgccatat cgggctgatg atgaaaacgg
      481 attttatgaa gagttttctg taaaaatgga agaagtgaga aaaaaactgg atggcttgaa
      541 agatttttcg taaagcgctt taaaatagaa aaaatgttct cttgtagttt tgattgtaaa
      601 gaagtataat gggaataaat ttatggataa cgcttcctga aagtatctca tggaagcgat
      661 ccggacagga aaacaggagg tttcgatatg gcttgggcaa tattttctat gctatttctc
      721 gcgatcggag gagtggtcta tggcgctgcg gcattaatct ggtttgcgtt gacgattctc
      781 gcaaactggc tgatgtttat gaaagcggga gaggccggtt ggaagtctat aattccggta
      841 tataatacat atacggctta taagatcgcg tggactccga atatgttttg gatttatttc
      901 atatttacag tgttggaaag tatattttcc agtatttcag gcggagattt ctggtcgttt
      961 tcgggatttc tgggaggtct gtgcggactg gtaacgatcg ttttgtctgt gttttattgt
     1021 gtgaatctgg cgaaaagctt cggaaaagga cttgggttta cgatcggact gattttcctt
     1081 gagccgattt ttgttatgat gatcgctttc ggatcagccc gatatcagaa atattaaaat
     1141 gtcttgtttt ttataagtga aaggcgtgtt atgaaaagag gctgtgaaat aacagtctct
     1201 atggaaaaag aggacctttc gttcaaaatg aacgataggt cctctttttt actccctttc
     1261 cgaaggggat atattgccaa aggagaagtt tatcagaggt atatccatca gagtgtggaa
     1321 atgaaacgtt taaaggcttt aattccttca tctgtgcatt tatatacacc tgcgtcttcg
     1381 agtactttgc aaaatacttt tccgacttcc tgttccaaaa tctgttcgat attttcttct
     1441 gtaacattgt cgtactctga tagaaaggca tctgtccagt ctgcgtgttt tgcaatttga
     1501 tcattgcttc ggatgtcttt tttctctatg atatattctt ttagaagttc catctctcct
     1561 ttgagtcttg caggaagaac ggcgagaccc attacttcta tcagaccgat gttttctttt
     1621 ttgatatggt gaagttcttc gtgcggatga tatactccga gaggatgttc ttctgttgta
     1681 atgttgttgc gaagcgtcag atcaagttca tacataccgt ctttttttct ggcgatcggg
     1741 gtgatcgtgt tgtgtaaaac cccgtctgtt tctgcaaata taaatgcttc ctcgtctgta
     1801 taagtacgcc attttgaaag aatatgatct gcaaggtcga taatctgttc cgtattttct
     1861 cctctcagac ggataacaga aaggggccat ttgacgattc ccgcttccac gtcttcatat
     1921 cccggcacag taaagttttc gatgatcgga gctttggcca tggcgaaagt ataatttccg
     1981 ccctggaaat gatcatggct taagatagag ccgccgacga tcggaaggtc tgcgttcgat
     2041 ccaaggaagt agtgtggaaa tagtttaata aaatcaaaca gtttggcaaa tgtatttctg
     2101 tctatcttca tcggaacatg ttcgccgtta aatacgatgc agtgttcgtt gtaataaacg
     2161 taaggagaat actggaatcc ccagctgcgt ccctggatcg tgatcgggat gatgcggtgg
     2221 ttctctcttg caggatggtt ggaacgtccg gcatagcctt cgttttccat gcagagctga
     2281 cattttggat aggaagcgga tttcgctttt cctgcagcgg caatcgcttt cggatctttt
     2341 tccggtttgg aaagattgat cgtaatgtca agtgtgccgt atttggtttc gactgtccat
     2401 tttcgatctt ttttgatgcg gtagcggcgg atatagtcac tgtcctgatt taatttataa
     2461 tagtaagagg tggcttcctc aggagaaatg ttatattttt cctgaaattc tttttggatc
     2521 tgtgaaggac gcggcatcag acagttcatg atttttgtgt caaaaagatc acggtatgta
     2581 atgctgtctt ctataattcc ccgttttacg gcctcgtcaa gaagatcttt gagaatttcc
     2641 tcaagatcac ggttgtcgga agtacattcg atgtcttcgt agttgtcttc atgaaagagt
     2701 tcaagaagaa gatttgttgt gtaaacacgt tcgcattcgg gagtaagacc ggtatctatc
     2761 ccatattgca ctaatttttt gattgattca taaagcatga tcggactcct tttttctgtt
     2821 ttttgttaat taatttgtgg aaagtttttt attttctcga aattttctgt ctatagtata
     2881 ggatgaaagc agtgaaaagg tctataaaaa atccgtgcga atccatgtaa aaatgttgca
     2941 tttataataa gggaaagttt ataatggata tagtttgatg atcgggatat gaaacgaagg
     3001 atcaagaaag gggattctat ggaagcatca tataaacatt cgtataaaac aggggaaaat
     3061 ctgctgaatt ctttgtccgt atataatgtc ggatatcaga aatgtgatcc cgaatatcag
     3121 tgggggcccg gagtgagaga tcactattgt attcatcaca ttttgtcggg gagcggatgt
     3181 tattgtacgg gaactgtgtc aaagcatttg gaagaaggag atacgtttat tctctatccg
     3241 ggagtagagc ttaggtatca ggcagatgcg gataagccgt gggaatatgc atgggcaggg
     3301 tttatgggcg cggatgcggc gtctcttatc agaaatacag aatttagcag ggagactccg
     3361 tatattttaa aagggaggat tccgacagaa aagatacgtg acggactgga acggatttat
     3421 aatgcgaaag gaaatacata tgaggccgct gtcgctatga caggggagat gtacagtctg
     3481 ctggcagtgt ttatgcatta cgcagagcat gaagagaaag aaaaggcgat acagctgact
     3541 tacgcagaga aggcggagag ttatatagaa acaaattatt catatcctat tacggtagaa
     3601 gagattgctt cctatgtcgg gataagcaga agccatttgt ttcgttcttt tcaaaattat
     3661 atgaacagat cgccgaaaga atatttgacg gaatacagga tcaagcaggc gtgtcgactt
     3721 ctgcgagaga cagatctttc ggtctcggcg attgcttatt cggtcggttt tgaaaataat
     3781 ctttattttt caaaagcttt tcgtaagcag aaaggggaaa gtccgtcaga gtacagaaag
     3841 agccgtgtaa gaaaaatatg acggtatatc ttgcaaaacg gaacgctttg catctataag
     3901 agaagaagaa catatactgg aaagtgaaag aaatcaaagg cagagatgca atggagcgaa
     3961 acagaaaaat atggatgcag atcttggtgt tggcagcagt gctgcgcttt tttccatttg
     4021 attttcaaac ggctgtttca acggcgtcag aagatgttgc ggcggaatgt gcagggggag
     4081 aagagcagaa accgaagatt gcgcttacgt ttgatgacgg gccgaatata aagtatactc
     4141 cgatgctttt ggacggttta aaagaacggg ggatcaaagc aacatttttt ctgatcggag
     4201 caaacacaga acaagaagaa agcaaaaaaa tcgtaaaaag aatgtatgaa gaaggtcatt
     4261 tgatcggaaa tcatacatat cgtcatgtcg atctgtcgaa aatcagcggc agagaggctg
     4321 aaaaagagat taaaactaca gatgaggcga ttaaaaagat cacaggacat gagacggaat
     4381 ttatccgtcc gccgtttggg gcaatgccgg aaggagaaga agatcccgat aaaatatatg
     4441 tgaaatggac ggttgactct cttgattggg ttacaaaaga cacggaaaaa attgtcgaga
     4501 aagtaacggc ggatacaaaa gaaggagatg taatccttat gcatgactgt tacggagagt
     4561 cggtggaggc cgcattacag attgttgata ttatggagaa acaaggatat gagtttgtga
     4621 cagcagacga attgattatt aattgatagt ttgaaagaac aggataggag aacggatgat
     4681 aaacgaatat tcaagaacag aacttttaat tggcagtgaa aatatggaaa aattaaaaaa
     4741 agcatctgtt gccgtgtttg gtgtaggagg cgtcggatct cactgtatcg aagcattggc
     4801 aaggtgcggt atcgggagat tgatattgat cgacaatgac gatgtatcgc ttacaaatat
     4861 taaccggcag tcgattgctt a
//
LOCUS       DBMDXF010000144         4835 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4274_length_4835_cov_2.455640, whole genome shotgun sequence.
ACCESSION   DBMDXF010000144 DBMDXF010000000
VERSION     DBMDXF010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_4835_cov_2.455640"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..4631)
                     /locus_tag="ACZPD4_06430"
     CDS             complement(<1..4631)
                     /locus_tag="ACZPD4_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1378733.1"
                     /translation="MKKKRVAALVMAAILGVTGFSVPGIGVVQAEEAHTEMSGEAEQQ
                     EINLAADRGSTARASKFLAASGNLPAREPGLAFDGISDNNGEADNSRWQSGEDAEFSE
                     QWLEVDLGGICVVSEIKVDFFARLYGDFRVEVSDSNAEDAVWTTIATADMPEGTDLNL
                     KKTVDVKENGKAREIPRYIRLYFTSGNSQAANRSIGVREFQVIGTKKSESGYETITGN
                     IALNKTASASGVEAAMPNLTANLAVDGQKSDTSRWSAPTMKNGTSPNQQQTPQWLEID
                     LRNEVTNITSIDLYFYKLVYSIDYEIQTRADKKSEWKTVKHVTCQPGNEQNKHDSITD
                     VEGKRLDRYVRFYFNKVNTNAGGNSVSVQEIEIHGDQVQTPEVTDPAPKNAKEAMDSV
                     KALEAITVDSETVPLPKMPDGFSISVKGSEYPQVISDEGQISDHNMYDYDMDVILEVV
                     NENDPEDTAEKTFQVHVPNKKSKHAEIYPEIKNQNEEPEVIPSLQEWYGYEGEVKLTE
                     NSRIVLKDGAGVGLEKVASQFKSDMKEITGMELEVVSGEGGDEDDILIESLPEDTYDT
                     GKEGYLLKADDGGIHISSNGYTGCLYGTKTLEQVYYTQDGTYSFPKGVTRDFSQYEVR
                     GVMIDIARVPYRLDALKDIVKTFSFYKINEVHFHLNDNRHSGATTGREDYNNWKNYKG
                     MFRLESKRFPSLKTDPQQNAYYNDENGAYGGEPQYTAEEYKDLQQFAMDCGMNPISEI
                     DAPGHSLLFNRYVRNNIEEIRKVQGFENMPEDGIKSDRDWELLSVKGESGQWALKFLK
                     ALYSEYLNEADPVFLGDTVDIGVDEYWSIKNDEFDGMRNYIREMADTVQESGKKVRMW
                     GSMKQYFDDKGISTKDYTDIEIDFWSNSWDNAAKRSAEGFKIVNVDSFHLYGNTGRDK
                     RDVVNVEHIFNNWTPVTFSSSGTVQPADPNLLGAKTAMWADIADMGVTERDNYERLMR
                     QAAVLSEKTWGGTDEDQTYEEYSLKFEKLKAGPGVELASDIPSETSLVLDYDFKNVKS
                     GEDGTVVYDAAGNGYNGTVINADVKEEDGKNWLDLNGDGSLTTGLRSVDYPYTVQFDL
                     KVDKKGDAQLFDGRDGRLSIGSDGKLKINRSYFEQKFDYTIPENKSVNVTIVGTQQVT
                     KLYINGEFKQALTRTTNSETDYNHLLSTFVFPLTTIGNGFDGKIADLKVYDKALSPKT
                     IKLAAKGKAVTEVNVAQDKAAAGTAQHKGDNNYDNANKKLRVGWKAIDGDGNTADGKH
                     GTDVSEKDSFFEGLYADSSFAVDMLQTHQIDHLVLQWDKAPATFKLQVSSDGKVWKDI
                     EGKASIKGESVNTIKFEQPLETRYIKMQGVDGTFQLREFEAYETVNKDHLKETLKAAD
                     DKLKEYGIQYGDEKYKEFFAAYMEAESAYENAYALNHNVSEKADALKAETEKLENLNP
                     KPEPTPELKSVTVTAESKKLKVGEETTVTAKVDPTDAKDVTITWKTSDATVLKVDEAG
                     KVTALKAGKATITATAVQGEKKVTDSVEIEVEEEVKPEPTPELKSVTVTA"
BASE COUNT         1119 a         1268 c          770 g         1678 t
ORIGIN      
        1 gccgttactg ttacactctt aagctccggc gtcggttctg gttttacctc ttcttcaact
       61 tcgatttcca cactgtccgt aactttcttt tcgccctgca ctgccgttgc cgtgatcgtt
      121 gcttttccgg ctttcaacgc tgttactttt cctgcttcat ctactttgag caccgtcgca
      181 tcagacgttt tccatgtgat tgtaacatct tttgcatctg ttggatcaac ttttgctgtc
      241 actgtcgttt cctctccgac tttcagtttc ttactctctg ccgttactgt tacactctta
      301 agctccggcg tcggttctgg tttcggattc agattttcaa gtttttctgt ctcagctttc
      361 aatgcgtctg ctttttcaga aacattatga tttaatgcat atgcattttc atacgctgat
      421 tctgcttcca tatacgctgc aaagaactct ttatacttct catcaccgta ctggatacca
      481 tactctttta acttatcatc tgctgctttg agcgtctctt tcaaatgatc tttatttacc
      541 gtttcatatg cttcaaattc tcttaactgg aatgttccgt caacgccctg catcttaata
      601 taacgtgtct caagcggctg ttcaaacttg attgtattta ctgattcgcc ttttatagat
      661 gcttttcctt caatatcctt ccaaactttt ccgtctgaag aaacttgaag tttaaatgtc
      721 gccggcgctt tatcccactg aaggacaaga tgatcgatct gatgtgtctg aagcatatct
      781 accgcaaaac tgctgtctgc ataaagacct tcaaagaaag agtctttctc actgacatct
      841 gttccatgtt ttccgtctgc cgtatttccg tcaccatcaa tcgccttcca gcctacacga
      901 agctttttat ttgcattgtc gtaattatta tcgcctttat gctgcgctgt tcctgctgca
      961 gctttgtcct gcgctacatt gacttctgtc actgctttac cctttgccgc caatttaatc
     1021 gtctttgggg acaatgcttt gtcataaact ttcaagtctg cgatctttcc gtcaaatccg
     1081 tttccgatcg ttgtcaacgg aaatacaaat gtagataaaa gatgattgta atccgtttct
     1141 gaatttgttg ttctcgtcaa tgcttgttta aattctccgt tgatgtaaag ttttgttacc
     1201 tgctgtgttc cgacgatcgt tacatttaca ctcttatttt caggaattgt ataatcaaac
     1261 ttctgctcaa aataagaacg gttgatcttt aattttccgt ctgatccgat cgacaaacga
     1321 ccgtctcgtc catcaaacaa ctgtgcgtct ccttttttat ccactttcag atcaaactga
     1381 actgtatatg gataatcaac agatcttaac cctgtcgtaa gacttccatc tccgttcaag
     1441 tcaagccagt ttttaccatc ttcttctttc acatctgcgt ttataactgt tccattatat
     1501 ccgtttcctg ccgcatcata tacgactgtt ccgtcctcgc ctgattttac atttttaaaa
     1561 tcataatcta atacaagcga tgtctctgaa ggaatatcgg atgccagttc cactcccgga
     1621 cctgctttta atttttcaaa cttgaggcta tactcctcat atgtctggtc ttcatccgtt
     1681 ccgccccatg ttttctcact aagaacagca gcctgacgca tcaatcgttc gtaattatca
     1741 cgttctgtca cacccatgtc tgcaatatct gcccacatcg cagtctttgc tccgagaaga
     1801 ttcggatctg ccggctgaac cgtaccgctg ctgctgaatg taaccggtgt ccagttattg
     1861 aaaatatgct ctacattgac aacatcgcgt ttatcccttc ctgtatttcc atacaaatgg
     1921 aaagaatcca cattgacgat cttaaatcct tcggcgcttc gtttcgccgc attatcccat
     1981 gaatttgacc agaaatcaat ttcgatatct gtataatctt ttgtggaaat tcctttatca
     2041 tcaaaatact gcttcataga tccccacata cgtacttttt ttccgctttc ctgaacggtg
     2101 tcagccatct cacggatgta attacgcatt ccgtcaaatt catcgttctt aatactccag
     2161 tattcgtcca ctccgatatc aaccgtgtct cctaaaaata caggatctgc ttcgttcaga
     2221 tattcgctgt acagcgcctt aagaaacttt aacgcccact gtccgctttc tccttttaca
     2281 gaaagcagct cccagtcccg gtcgctttta attccgtctt ccggcatatt ttcaaaacct
     2341 tgtacttttc tgatttcctc gatattgttt ctcacatatc tgttaaataa caacgaatgt
     2401 cccggcgcat cgatttccga aatcggattc atgccgcaat ccatcgcaaa ctgctggaga
     2461 tctttatatt cttctgctgt atactgcggt tctccgccgt aagctccgtt ttcatcattg
     2521 taatatgcat tctgctgagg atctgtcttt aaactcggaa accttttgct ttccaaacgg
     2581 aacattcctt tataattttt ccagttatta taatcttcac gtcctgtcgt agctcccgaa
     2641 tgacgattat cgttcagatg aaaatgtact tcattaattt tataaaatga aaacgtcttc
     2701 acaatatctt tcaaagcatc caaacgatac ggaacacgcg cgatatcgat cattactccg
     2761 cgtacttcat attgagagaa atctcttgtc acacctttag ggaaagaata cgttccgtct
     2821 tgtgtataat acacctgctc taacgtcttt gttccgtaca gacatccggt gtatccgtta
     2881 ctgctgatat gaattcctcc gtcgtctgct ttcagtaaat atccttcctt gcctgtgtcg
     2941 tatgtatctt ccggcagtga ctcaatcaag atatcgtctt cgtctccgcc ttctccgctg
     3001 acaacctcaa gttccattcc ggtaatttct ttcatatcgc tcttaaactg agacgctact
     3061 ttctcaagtc cgactcctgc gccgtccttt aaaacaatac gggaattttc cgtaagctta
     3121 acttctccct catatccata ccattcttga agacttggaa tcacttccgg ttcttcattc
     3181 tgatttttta tctctggata aatctctgca tgttttgatt ttttattcgg cacatgaacc
     3241 tgaaatgttt tctctgccgt atcttcagga tcattttcat tcacaacttc caatatcaca
     3301 tccatatcat aatcatacat attatgatca ctgatctgac cctcatcaga aataacctga
     3361 ggatattcgc ttcccttcac actaatactg aatccatcag gcatcttcgg aagcggaact
     3421 gtctcagaat ctaccgtgat cgcctcaagc gcttttacag aatccatcgc ttcttttgca
     3481 tttttcggcg ccggatctgt cacctcgggt gtctgtacct gatccccatg tatctcaatt
     3541 tcctgaacag aaacactgtt gccgcccgcg ttcgtattta ctttattgaa atagaaacgc
     3601 acataacggt caagacgctt tccttctaca tctgtgatac tatcatgctt attctgctca
     3661 tttcccggct gacatgtcac atgtttaaca gttttccact ctgatttttt atcagccctt
     3721 gtctgaatct catagtcgat cgaatataca agtttataaa aataaaggtc gatgcttgta
     3781 atattcgtaa cctcatttct cagatctatc tcaagccact gcggggtctg ctgctgattt
     3841 ggtgaagttc cgtttttcat tgtaggtgca gaccatctgg aagtatccga cttctgtccg
     3901 tcaactgcaa gatttgcagt cagattcggc attgccgcct ctactcctga cgcagacgcc
     3961 gtcttattca atgcgatatt tcctgtgatc gtctcataac cgctttcact tttcttcgtt
     4021 ccaatcacct gaaattcccg caccccgata ctgcggtttg ccgcctgact gtttcctgat
     4081 gtaaagtaaa gccttatata gcgtggaatc tctctcgctt ttccgttttc cttcacatca
     4141 acagtttttt tcaagttcag atctgtacct tccggcatgt ctgcagtcgc aatcgttgtc
     4201 cacactgcgt cttctgcatt ggaatcagaa acttccacac ggaaatctcc gtacagcctt
     4261 gcaaagaaat caaccttgat ctctgataca acgcaaattc ctcccaggtc tacttccagc
     4321 cactgctctg aaaactccgc gtcctctcca ctttgccatc tgctgttatc cgcttctccg
     4381 ttattatcgg aaattccgtc aaacgcaagc cccggttctc ttgcgggaag gttgcccgat
     4441 gccgcaagaa acttagatgc ccgcgcagtg cttcccctgt ctgccgcaag atttatttct
     4501 tgctgttcag cctctccact catttctgtg tgagcttctt ctgcctgcac aactccaata
     4561 cccggaacag aaaatcctgt cacaccgagt attgctgcca ttacaagcgc cgctactctt
     4621 ttctttttca tattattctt tttctccttc tatgtatcca tctctcagcc cagctttaat
     4681 catccggaca cacggataca gttatttcta taataatcct ttcaacttgt ttttgtctac
     4741 actttttaat atttccacct gtttttaaat aattttgtgc attttttaca taactttatt
     4801 attttgatac agtaaaaaac cgggtgcatt atgca
//
LOCUS       DBMDXF010000145         4791 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4302_length_4791_cov_1.768657, whole genome shotgun sequence.
ACCESSION   DBMDXF010000145 DBMDXF010000000
VERSION     DBMDXF010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4302_length_4791_cov_1.768657"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..44)
                     /locus_tag="ACZPD4_06435"
     CDS             complement(<1..44)
                     /locus_tag="ACZPD4_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012267.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378734.1"
                     /translation="MGCGNCADVCPGNP"
     gene            complement(31..240)
                     /locus_tag="ACZPD4_06440"
     CDS             complement(31..240)
                     /locus_tag="ACZPD4_06440"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1378735.1"
                     /translation="MYYELYIDVFFLENFMLDSLLLMAVNRVMQYRNSYARMLAGGAA
                     GSFLTCVVIALPLMPAIKTLLSGLW"
     gene            complement(428..1621)
                     /gene="ftsZ"
                     /locus_tag="ACZPD4_06445"
     CDS             complement(428..1621)
                     /gene="ftsZ"
                     /locus_tag="ACZPD4_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1378736.1"
                     /translation="MLEIKTNESEAAARIIVVGVGGGGNNAVNRMIDEQIAGVEFIAV
                     NTDKQALQLCKAPTLMQIGEKLTKGLGAGAQPEVGEKAAEESSEEISAALKGADMVFV
                     TCGMGGGTGTGAAPVIARIAKEQGALTVGVVTKPFRFESKTRMQNALSGIDKLKENVD
                     TIIVIPNDKLLEVVDRRTTMPEALKKADEVLQQGIQGITDLINVPSLINLDFADIQTV
                     MKDKGIAHIGIGAGRGDDKALEAVKEAVASPLLETTIQGASNVIVNVSGDITLMDASD
                     AADYVQELAGEGASIIFGAMYDDAKSDECTITVIATGLHNVGGSASKLKARLEGQQKV
                     GSILPSGESTVRSRLDNDGSRVSAARPQTSARPQGGTMPTLQPRTPSSTVKEQSIKIP
                     DFFKK"
     gene            complement(1781..2524)
                     /locus_tag="ACZPD4_06450"
     CDS             complement(1781..2524)
                     /locus_tag="ACZPD4_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1378737.1"
                     /translation="MKRKKHKKRKVWPIILVVLTVLIVMAAAAVFLFRTRSYKVEGNS
                     YYGERTITTWIENDPLSVNSLYVLYKYNFTDADLPSGVESLSISLKDPWTVKVKVKEK
                     EMAGYVDYDGAYLYFDRTGTAVLRTKKIIEGVPHIEGLMFDSAKAKIGKKLPVEDDSI
                     FEKIVEVSKNLKKYNLTPDRMGCTDGGVQIYFEIVQVSLGNGNYEEKLRQVEPILKKL
                     TELYPGKAGVLHLENYDAESESIPFVPAA"
     gene            complement(2525..3595)
                     /locus_tag="ACZPD4_06455"
     CDS             complement(2525..3595)
                     /locus_tag="ACZPD4_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1378738.1"
                     /translation="MLIFVVLLLVAAGLVILYSTSAYNGQVKFHDPFHYLKKQGFATL
                     LGLFGMALIARVDYHKWVPLAIPAYVTSIILSVAVIFFGDEYNGSKRWLSLGPISFQP
                     SEFAKVAVIFFLACLVSRNVQRMKRFRTMILMMIPVLPIVGLVGASNLSTAIIILGIA
                     VVLIFVADPKYVRFILMGSIGVGFMTVFLAMESYRLERLAIWRHPEQYEKGYQTLQGL
                     YAIGSGGLFGRGLGNSVQKLGFLPEAQNDMIFSIICEELGLIGAGFIILLFLILIWRF
                     FVISTKASDLLGALIAAGAMAHMMIQVILNIAVVTNSIPNTGITLPFISYGGTSVVFL
                     LLEMGLVLSVSNYEGLRYGGSE"
     gene            complement(3645..>4791)
                     /gene="murD"
                     /locus_tag="ACZPD4_06460"
     CDS             complement(3645..>4791)
                     /gene="murD"
                     /locus_tag="ACZPD4_06460"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1378739.1"
                     /translation="LSFVVMSPGVPTDLPIVEKMREKSIPIWGEIELAYVFGKGDVLA
                     ITGTNGKTTTTALLGQIMQDYKEHAYIVGNIGTPYTSVAQETTDDAVIVAEISSFQLE
                     TIHTFHPKVSAILNITPDHLNRHHTMEAYIEAKERIAENQTKADVCVLNYEDDELQKF
                     GEKTAAQVLYFSSARKLEKGIYLDGTKIICAADVPVELCDVSELKILGTHNYENVMAA
                     SAMAYAYGVPIEVIRKNLLTFPGVEHRIEFVAEKRGVLYYNDSKGTNPDAAIKGIQAM
                     NRPTILIGGGYDKDSEYDEWIKAFDGKVRKLVLLGATREKIAQSAHANGFDDIIMADT
                     FEEAFEQCVKYAKPGDAVLLSPACASWGMFKNYEERGDKFKELVDQL"
BASE COUNT         1306 a         1244 c          804 g         1437 t
ORIGIN      
        1 ttcggattgc ccggacaaac atcagcacag ttaccacaac ccagacagaa gtgtttttat
       61 cgccggcata agcggaagcg ctatcacaac acaagtcaaa aaactgcctg ctgcccctcc
      121 tgcaagcatc cgcgcataag aattcctata ctgcataaca cggtttacag ccattaaaag
      181 cagactgtct aacataaaat tttctaaaaa aaatacatct atgtataatt catagtacat
      241 agtccacctt ctttctcttc cgtaattatg tagtgcggaa tctattatat atttagaaac
      301 aggaaaattt tgtcatatat tgacatgtat ctgtaaatat atttcgacaa aaaacaaaaa
      361 aacagaatgg aactcttaca tgaaattcca ttctgtctta aaattctata ttattatacc
      421 gattcgctta tttcttaaag aaatccggaa tcttaataga ctgctctttc accgtactgg
      481 atggcgtacg cggctgtaaa gtcggcatcg tacctccctg cggtctggca gatgtctgtg
      541 gtctggccgc agatactctg cttccatcat tgtcaagacg acttcttact gtcgattcac
      601 cggacggaag gatcgatccg actttctgct gtccttccaa tctcgccttt aattttgatg
      661 cgcttccgcc tacattatgt aacccggtcg caataacagt gatcgtacat tcatctgact
      721 tcgcgtcatc atacatagca ccgaaaataa tacttgcgcc ttctcctgca agttcctgta
      781 cataatcagc cgcatcagaa gcatccataa gagtaatatc tcccgatacg ttaacgataa
      841 cattcgacgc accctgaatc gtcgtctcaa gcagcggact cgcaacagct tcttttaccg
      901 cctcaagagc cttatcgtct ccgcgtcctg caccgatacc gatatgagca atgcctttat
      961 ccttcataac tgtctgaata tccgcaaagt caaggttgat cagtgacgga acattgatca
     1021 aatctgtgat accttgaata ccctgctgaa gaacttcatc agctttctta agagcttccg
     1081 gcattgttgt gcgtctgtca acaacctcaa gaagcttgtc attcggaatt acgataatcg
     1141 tatctacatt ttcttttaat ttatcaattc cgctcaatgc attctgcatt cttgttttcg
     1201 actcaaaacg gaaaggtttt gtgacaactc cgactgtaag agctccctgc tcttttgcaa
     1261 tacgtgcaat aacaggtgca gcacctgtac ccgttccgcc gcccataccg catgttacga
     1321 acaccatgtc cgcacctttg agcgctgccg agatctcttc tgaactttcc tctgccgctt
     1381 tttctcctac ttccggctgt gcccctgcgc cgagcccttt tgtaagcttc tcaccgatct
     1441 gcattaatgt cggtgcttta caaagctgca gtgcctgctt atctgtattc actgcgataa
     1501 attccacgcc cgcgatctgt tcgtcaatca tccggtttac tgcgttgttt ccgcctccac
     1561 cgactccgac tacaataatt cttgcggccg cttctgattc gttggtttta atttctaaca
     1621 agagtacttc ctccttcgtc gtcttactaa tttcttatac atcccttaat attgaacgat
     1681 agaattcaat aagtatataa taaagtcttt ttagcaaata atcaatagat tttttgctat
     1741 ttttctgaat tattacactt ttacagatga tccgccgccg ctatgccgcc ggaacaaacg
     1801 gaatcgactc cgattcggca tcgtaattct ctaaatgcag cacgcccgcc tttccgggat
     1861 ataattcagt cagtttttta agaatcggtt ctacctgtct aagcttttct tcataattcc
     1921 cgtttccaag gctgacctgt acaatttcaa aatatatctg tacgcctcca tctgtacacc
     1981 ccatcctgtc aggtgtgaga ttgtattttt ttaaattttt tgaaacttct acaatttttt
     2041 caaaaatact gtcatcttcc accggaagtt ttttccctat tttagctttt gccgaatcaa
     2101 acataagacc ttcgatatga ggaacacctt cgattatttt ttttgtccgc aatactgccg
     2161 ttccggttct gtcaaaataa agatatgcac cgtcataatc tacatatccg gccatttctt
     2221 tttctttcac tttcaccttc accgtccacg gatctttcag tgaaatactc agactttcta
     2281 ctcctgacgg aagatcggca tcggtaaaat tatatttata cagaacatac agagaattta
     2341 ccgaaagcgg atcattctca atccatgttg tgatcgttct ctctccgtaa taagaatttc
     2401 cttccacttt atatgacctt gtgcgaaata gaaataccgc cgcagctgcc ataacgatca
     2461 agacagtaag cacaactaaa ataatcggcc acacctttct ctttttgtgt ttttttctct
     2521 tcatctattc tgaacctcca taccgtaaac cttcgtaatt cgatacgcta agcacaagtc
     2581 ccatctccaa cagaagaaat acaaccgatg tacctccgta actgataaac ggaagcgtaa
     2641 tccctgtgtt tggaattgaa tttgtaacta ccgcgatgtt taatataacc tggatcatca
     2701 tatgcgccat tgcccctgcc gctatcaaag cgccaagcag atccgacgct tttgttgaaa
     2761 tcacaaaaaa acgccaaata agaatcaaaa ataaaagaat gataaatcct gctccgatca
     2821 atcccaattc ttcacagata atagaaaaaa tcatatcatt ttgagcctct ggaagaaatc
     2881 cgagtttttg tacactgttg cctaatcccc ttccgaacag acctccgctg ccgattgcat
     2941 atagtccctg cagcgtctga tacccttttt catattgctc cggatgtctc cagattgcca
     3001 ggcgttccaa tcgataactt tccatcgcca gaaaaaccgt cataaatcct acgccgatac
     3061 ttcccatcaa tataaaacgg acgtatttcg gatccgctac aaaaatcaga acaaccgcaa
     3121 ttccgagaat gatgatcgca gtgcttaaat tacttgctcc gaccagacct acgatcggca
     3181 aaaccggaat catcataagt atcattgtgc gaaaacgttt catccgctgt acatttctgc
     3241 tcacaaggca tgcaaggaaa aaaataacgg ctacttttgc aaactctgaa ggctgaaaag
     3301 aaatcgggcc caaagacaac catcttttgg acccgttgta ttcatccccg aaaaatataa
     3361 cagccacaga cagaatgatc gacgtcacat atgcgggaat cgccagcgga acccatttat
     3421 gataatcaac gcgggcgata agcgccatac caaacagtcc tagcagtgtc gcaaacccct
     3481 gctttttcaa ataatgaaac ggatcgtgaa atttcacctg cccattgtaa gcgcttgtgc
     3541 tgtacaatat cacaagccct gcggcaacaa gaagcaatac aacaaatata agcatcccgt
     3601 catatcgttt tttcttttca gaacgcgtca atcagctcca ctccttatag ttggtctaca
     3661 agttctttga atttatctcc acgttcttca taatttttaa acatccccca gcttgcacac
     3721 gccggcgata aaagcaccgc atcgcccggc ttcgcatatt tcacgcactg ttcaaacgcc
     3781 tcctcaaagg tatctgccat aataatatcg tcaaatccgt ttgcatgagc gctttgcgcg
     3841 atcttctctc tcgttgcgcc aagcagaaca agttttctca ctttcccgtc aaacgccttg
     3901 atccactcat catattcgga atctttgtcg taacctcccc cgatcagtat ggtaggacgg
     3961 ttcatggcct gaatcccttt gatcgctgcg tccggatttg ttcctttcga gtcattataa
     4021 taaaggacac ctcttttttc tgccacaaac tcaattcgat gttccactcc gggaaatgtc
     4081 agaagatttt tgcggatcac ctcgatcgga acaccatatg catacgccat tgcagaagca
     4141 gccatcacat tttcataatt gtgtgttccg agaatcttca gttcactgac atcgcaaagc
     4201 tcgaccggaa catctgccgc gcaaatgatt tttgttccat caagatatat ccctttttcc
     4261 agtttacgcg cactgctgaa atatagaact tgcgctgccg ttttttctcc gaatttctga
     4321 agttcatcat cctcataatt caatacgcac acatctgctt ttgtctgatt ttcagcaata
     4381 cgctctttcg cttcgatata tgcctccatt gtatgatgac gattcaaatg atcgggagta
     4441 atattcaaaa tcgcgcttac tttcggatga aatgtatgta ttgtctcaag ctggaaactg
     4501 cttatttccg ctacaattac cgcgtcgtcc gtcgtttctt gtgcaacgct tgtatacgga
     4561 gttcctatat ttcctacaat ataagcatgt tctttatagt cctgcatgat ctgtcccaaa
     4621 agggctgtcg tcgtcgtttt cccgtttgtt cctgtaattg caagaacgtc tccttttcca
     4681 aacacatatg caagttcgat ctctccccag atcgggatgc tcttttcacg cattttttca
     4741 acgatcggaa gatccgtcgg cactcccgga ctcatcacta caaatgaaag c
//
LOCUS       DBMDXF010000146         4708 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4399_length_4708_cov_1.763186, whole genome shotgun sequence.
ACCESSION   DBMDXF010000146 DBMDXF010000000
VERSION     DBMDXF010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4399_length_4708_cov_1.763186"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            228..1406
                     /locus_tag="ACZPD4_06465"
     CDS             228..1406
                     /locus_tag="ACZPD4_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1378740.1"
                     /translation="MKMWKKAASIALVSALTVSMAACGNGGGSDKGSADADTFKIGGI
                     GPTTGDAAIYGTAVQNGIQLAIDEINEAGGINGYQIEFKFEDDQSDSEKSVNAYNTLK
                     DWGMQMLVGTTTSTPCTAVVEETHADNMFQLTPSATAVESIQYDNAFRMCFSDPDQGK
                     ASADYISENNLAKKIAVIYNSSDTYSSGVYQKFAEQAKTLGLDIVAAEAFTADSKTDF
                     SVQLQKAKDAGAELVFLPIYYQEASLILAQANKMGFAPKWFGCDGMDGLLALDGFDAA
                     LAENLMFLTPFTADAEDEATQKFVSDFEAAFKGTPNQFAADAYDCVYVIKEAAEKAEL
                     TPDMSVSDMSDALKKAMTEIKVDRMTGKSITWSEDGEPTKDPTVVIVQGGVYKILHAE
                     "
     assembly_gap    1511..1520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1681..2244)
                     /locus_tag="ACZPD4_06470"
     CDS             complement(1681..2244)
                     /locus_tag="ACZPD4_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1378741.1"
                     /translation="MNQRLTASQAEKTKKITMIGLMTAVTCILGPLSLPLPFSPVPIT
                     LTNFAVFLSIYILGMKYGTISLLIYLALGTIGLPVFSSFSGGLGKLAGPTGGYLFGFI
                     FLALIHGFFMLRFHKKTFASIIGMTLGMIACYLFGTIWLALHMSLTFKAALMIGVIPY
                     LPGDAVKIALAAIIGPKLSSAIQKTQK"
     gene            2630..3466
                     /locus_tag="ACZPD4_06475"
     CDS             2630..3466
                     /locus_tag="ACZPD4_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR1378742.1"
                     /translation="MFGYVTVCEPELKVKDLKKYKAYYCGLCHVLKEEYGFIGQMTLT
                     YDMTFAIILLSSLYESDTRADMHCCKVHPVKKQIMLTNEITSYGAAMNVLMAYYHMED
                     DWQDERKVSSLTVKTMLRGKVKKIMEQYPRQSRAIESALNELSVCEREGSTDLDKTAG
                     CFGKLMEELFLYKKDRWEETLRKMGFFMGKFIYLMDAYEDLPNDLEKGCYNPLKKIAE
                     EEGYEERIKQILCMMIAESTAEFEKLPCLSDIDILRNILYDGVWNCYNKIQRKKSEEQ
                     KK"
     gene            3463..4116
                     /locus_tag="ACZPD4_06480"
     CDS             3463..4116
                     /locus_tag="ACZPD4_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR1378743.1"
                     /translation="MINNPYEVLGVSPSASDDEIKKAYRDLTRKYHPDANVDNPLADL
                     AEEKFKEVQEAYDMIMKERASGGGYSYGYGSSSGSSSYGAGGNYQNAQQDPRLQAAVN
                     YINSRRFREALNTLDQINERSAIWYYLSSCANAGLGNNVLARDHAAQAVNMEPNNMQY
                     RQLLNQLNYASSRYQNSSYGNGYSSGTTPCGTGNMCCDLFVADSLCECMGGDLCSCM"
     gene            4107..4640
                     /locus_tag="ACZPD4_06485"
     CDS             4107..4640
                     /locus_tag="ACZPD4_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378744.1"
                     /translation="MHVKAKTMAFSGLLLALTVIFMVLGSVIETSTLFLLAAASFFVG
                     IIIREFGLRIGAAFYAAGVILGFITAPNKFYVFTFAAMGLYIWGREAAWRWIVRHVRE
                     EKSRTAFLLFKYGIFNLVYIPIVVIFREMLFAKAVSTPILVGVMIAGQIGILIYDKAY
                     DYVQVRLWGKMRGRVMS"
BASE COUNT         1504 a          737 c         1205 g         1252 t
ORIGIN      
        1 atgggattgc agcgattgtc atagtggcgg cagttattgt tacgattgct tcgtggtttt
       61 ggagtcagcg gattttagaa aaaaaagagt tttaaatcag cggtttgatt ttatcggtga
      121 aaaagcttga gggaaaaaac agagtacaat ctgttttttc tcttgacaaa actgcttctg
      181 caagttacca tagtaacaca gtaagttagc aaggaggagt tttagttatg aaaatgtgga
      241 agaaagcagc aagtatcgcg cttgtatcag ctctgacagt atcaatggcg gcttgcggaa
      301 acggcggcgg aagtgacaag ggttcggcag acgcggatac atttaagatc ggaggtatcg
      361 gacctacaac aggagatgcg gcgatttatg gtacggcagt acaaaacggt atccagcttg
      421 cgattgacga gatcaatgaa gctggcggta tcaacggata tcagatcgaa tttaaatttg
      481 aggatgacca gtctgattcc gaaaagtcgg ttaatgcgta caatacgctg aaagactggg
      541 gaatgcagat gttagtcgga acgacgacat caacaccgtg tacggcagtt gtagaagaga
      601 cacatgcaga taatatgttc cagctcacac cgtcggcaac ggctgtagaa agtattcagt
      661 atgacaatgc attccgtatg tgcttctcag atccggatca gggaaaagca tcggctgatt
      721 atatctcaga gaataatctg gcgaaaaaga tagcggttat ttacaacagt tccgacacat
      781 attcatcggg agtttatcag aaattcgcag agcaggcgaa aacactcgga cttgatattg
      841 tagcggcaga agcgtttacg gcagacagta agacagactt ttccgttcag cttcagaaag
      901 cgaaagacgc aggcgcagaa cttgttttcc tgccgattta ttatcaggag gcatctttga
      961 ttcttgctca ggcaaacaaa atgggatttg caccgaagtg gttcggatgt gacggaatgg
     1021 acggactgct tgcattagac ggatttgatg cagcgttggc tgagaattta atgttcctta
     1081 caccgtttac ggcagatgcg gaagatgaag cgacacagaa atttgtatcc gattttgaag
     1141 ctgctttcaa gggaactccg aatcagtttg cagcggatgc ttatgactgc gtatatgtga
     1201 tcaaagaggc agcagaaaag gctgaactta cacctgatat gagcgtttcc gatatgagtg
     1261 atgctttgaa aaaagcgatg acagagatca aagttgacag aatgacagga aaatcaatta
     1321 catggagtga agacggagaa ccgacaaaag atccgacagt tgtaattgtt cagggcggag
     1381 tttataaaat ccttcatgca gaataaaaag aaaaacagaa gcagaacggt tatgttatgt
     1441 gaaagtgctt cttatatgtg agaaaaagat cctgcgttaa gcgggatctt tttgtatagg
     1501 acagtttgtt nnnnnnnnnn gagttaaagt agagtgcttt tgacacttca actttatgag
     1561 gggaagatag ttgcttatca actatctaac tcagattata aagaacaaat atgtcaaaag
     1621 tatgtttaaa ctataaaaga atacgaaaaa ttataaacag ggaattaaga aagtatataa
     1681 tcatttctgt gtcttttgta ttgcgcttga tagtttcggt ccgatgattg ctgcgagagc
     1741 gatcttgaca gcatctccgg gaagataagg gatgactccg atcataaggg cagctttgaa
     1801 agtaaggctc atatgtagtg caagccatat cgttccgaat agataacagg caatcatgcc
     1861 gagagtcatg ccgatgattg aagcaaacgt ttttttatga aatctcagca taaaaaatcc
     1921 atggatgagt gcaaggaaga tgaaaccgaa caaatatccc ccggtcggtc cggctagctt
     1981 cccaagaccg ccgctgaacg acgaaaagac aggaagtccg attgtaccga gagcaaggta
     2041 aatgagcaga ctgatcgttc cgtatttcat tccgagtata taaatagaaa gaaaaaccgc
     2101 aaagtttgtc agagtgatcg gcacaggact aaatggaagc gggagggaaa gaggtccgag
     2161 aatgcatgtt acagctgtca taagtccgat cattgtgatt ttttttgttt tttctgcctg
     2221 tgaggcagtg aggcgctggt tcataagatt actcctttac gtaaaatgta tggtttgata
     2281 aaaaagaacg aaagcacata aaaactgtta tgaaaaagaa cacggtttta ttggtgtatc
     2341 cgagcgtagt ataatagaaa atgaaacaaa agtcaaccta aaataaaaac aggtttacaa
     2401 ttagaatctg acgttgaaaa tactttataa aacattgtaa aggctattta tagttaatat
     2461 cagcaagctg tgtaacagta cacagttata attcaccgta tttttataaa cagcaggccg
     2521 aatgcgtgtt ataatgcgat aagaatgtga cggaaaatat tgttttacaa tgaaagcatg
     2581 tcaaagcaag acaatgtaga aaatgaagaa gtgtttgagg agaagagtga tgtttgggta
     2641 tgtgacggta tgtgaaccgg aattaaaggt gaaagattta aaaaaatata aggcatatta
     2701 ttgcggtttg tgtcatgtat taaaagaaga gtatgggttt ataggtcaga tgacacttac
     2761 atatgatatg acatttgcaa ttattttgtt atcttcgcta tatgaaagtg acacgagagc
     2821 agatatgcat tgctgcaaag tgcatccggt aaaaaagcag ataatgttga caaacgagat
     2881 tacgtcatat ggtgcggcca tgaacgtgtt gatggcatat tatcatatgg aagatgattg
     2941 gcaggatgaa aggaaagttt caagcctgac tgtaaaaacg atgcttcgag gtaaagtaaa
     3001 aaaaatcatg gaacaatatc cgaggcagag cagggcaatc gaatcggctc tgaatgaatt
     3061 gtctgtttgc gaaagagaag gaagtacaga tttggataaa acggcaggat gtttcgggaa
     3121 actgatggaa gagctgtttt tatataaaaa agaccggtgg gaagaaactc ttcggaagat
     3181 gggatttttt atgggaaagt ttatttatct tatggacgca tatgaagatc tgccgaatga
     3241 tctggaaaaa ggatgctata acccgttgaa gaagattgcc gaagaagaag gatatgaaga
     3301 gcggatcaaa cagatcttat gcatgatgat agcagagagt acggcagaat ttgagaagct
     3361 tccatgcctt tcggatatag acattttgcg gaatatattg tatgatggag tgtggaattg
     3421 ttataataag atacagagaa agaaaagtga ggaacagaaa aaatgataaa taacccgtat
     3481 gaagtgctcg gcgtttctcc aagcgcgtca gatgatgaga taaaaaaagc atatcgtgat
     3541 cttacaagaa agtatcatcc ggatgcgaat gttgataatc ctcttgcaga tcttgcagaa
     3601 gaaaagttta aagaagtaca ggaagcttat gatatgatca tgaaagagag agcttcgggc
     3661 ggcggatatt cttatggtta cgggtcgtcc tcgggcagtt cttcttatgg tgcgggcggt
     3721 aattatcaga acgcacagca ggatccgagg cttcaggcgg ctgtgaatta tattaacagc
     3781 agaagatttc gcgaagcgct caacacgctt gatcagatta acgaacgatc tgcaatctgg
     3841 tattatttaa gcagctgtgc gaatgcggga cttggcaata atgttctggc aagagaccat
     3901 gcggctcagg ctgtgaatat ggagccgaac aatatgcagt acagacagct tttgaatcag
     3961 ttgaattatg cgagcagccg ttatcagaac agctcttatg gcaatggata cagcagcggt
     4021 actacaccgt gcggtacagg caatatgtgt tgtgatctgt ttgttgcgga ttcgttgtgc
     4081 gaatgtatgg gaggcgactt gtgttcatgc atgtaaaggc aaaaacaatg gcgtttagcg
     4141 gacttcttct tgcgctgact gtgatcttta tggtgttggg cagtgtgatc gagacgagca
     4201 cattatttct gctggcggcg gcatcttttt ttgtcgggat cattatccgg gagttcggac
     4261 ttaggatagg agcggcattt tatgcggcag gagtgatact tggatttatt acagcgccga
     4321 ataagtttta tgtgtttaca tttgcggcga tgggattata catatggggg agagaagctg
     4381 catggagatg gatcgtgcgg catgtgcggg aagaaaagag caggacggcg tttcttcttt
     4441 ttaaatacgg aatctttaat cttgtatata tcccgatcgt tgttattttc agagaaatgt
     4501 tatttgcaaa agcagtatcg accccgattc ttgtcggtgt tatgatagcc ggacagatag
     4561 gaatacttat ctatgataag gcatatgatt atgttcaggt tcgcctttgg ggaaagatgc
     4621 gcggaagagt gatgtcataa agaacgatat aaaaaggatg agccacattt ctgcggctca
     4681 tgggggaaaa agtttatgaa aaagtgtt
//
LOCUS       DBMDXF010000147         4690 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4415_length_4690_cov_2.033776, whole genome shotgun sequence.
ACCESSION   DBMDXF010000147 DBMDXF010000000
VERSION     DBMDXF010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_4690_cov_2.033776"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..606)
                     /locus_tag="ACZPD4_06490"
                     /pseudo
     CDS             complement(1..606)
                     /locus_tag="ACZPD4_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(741..>4690)
                     /locus_tag="ACZPD4_06495"
     CDS             complement(741..>4690)
                     /locus_tag="ACZPD4_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-alpha-N-acetylgalactosaminidase family
                     protein"
                     /protein_id="HIR1378745.1"
                     /translation="ISVKDNDLTWEITKIDRKEGTDKIASIDIPQLNLLSVDQVEENA
                     SFAGAVKSTDTKKSGDKFITFDDGFVAQKSVGYVYGFLTNKNLSAGLFSNSEAEDDLR
                     VIMNSGADTMSLTSAQWYYEAGDKGGQAQAATYDYPLSELPYAKVCIAEDMNEDKTID
                     WQDAAVAYRDIINVPYGSEDVKDLVNYRIVMNFGSAVTNPYSVTADNIKKVALATDGL
                     PQAVMLKGYGNEGHDSANSEYADISEREGGVDDFRDLLDVAHEYDTEIGIHVNAQEAY
                     PEARSFNDDMIMGPQQGGWGWLDQSRVINKIWDLGTQARYKRFAQLFDRINNTNLLSL
                     DWDKGEYVKDSQGTLASADEIAAAVKKAGADNMDFIYLDVWYQNAWETRRIAEEINSL
                     GWRFSTEFPYEGEYDSTWSHWATEGPYGGSATKGLNSDIIRFLRNDMRDVQVLNWVGH
                     GGAADNPLLGGYKLEGFEGWGGDQDFNNYLYLTFAENLPTKFLQHYQVIDWENYAEGE
                     SPVGNQEKQITLKNGDDKVVVTRNEAQRSDEIIEREITLNDKTVLSATNDHFYYLLPW
                     TDSDTKETKLYHYNLEGGTSTWNVPDGFTGNLTMYELSDQGRGEAIAVPVSNGQITLE
                     AKAKTAYVIVTDGKEMPTADTVNFGETTSVKDPGFNSYADGDKVDADVWSGADNDAVK
                     VTKAWTGDQRLTIESPAEDVAVSTKLTGLKAGKDYVAEVYVDNRSDSKATVEVNAGTI
                     KESAYTTKSIAQNYVASDQKHAGGNEASYMQRVYVPFTAEKETATLTISRAAGDGATY
                     FDDIRIIDKKIENYKEDGSFEQDFESVTSGYYPFVLGPAQRGGDGQLHLSQDNGKYTS
                     VGWGGYVINDVIEGEWSLKQHCDSGSGSGRTGLLYHTIPQNFRFEAGKTYKVEFDYEA
                     GSSAYAVVLGDGERFNNATDNDYLAVTAGTGKASHHEFTVTGSESGQTWFGIYANGAK
                     QPGGAYGQQDLILDNLKITPVKSGEVDTTKLEAELNNADKVVLDAFEDGKEKAAFEAA
                     YAAAQKVLENPTDQAAVDKAEKDLEDARKALLATSPKPEEDKEAPTKPSDLKETETTT
                     TTVKVEWKASTDNVGVTGYNVYADGKLAETVKGDVLTAELKDLKAGTEYTIEVEAFDA
                     AGNKSEKASVKATTKEEQKPDPNPEEDKEAPTKPSDLKVTDKTDTTVKIEWKASKDNV
                     GVTGYKIFVNGKSIGTVKDDVLTAEIAKLTPKTEYTIEVVAYDAAGNKSAAASVKATT
                     KEGKTPETPTTPEVKPGTVQTGDATPFAVPFMMMFIAVAMFAGVSVIRVKKRK"
BASE COUNT         1030 a         1180 c          866 g         1614 t
ORIGIN      
        1 caatacgttt ctgaaggggc gtttttcttt taccgattcc atgaacaaac tcgatatttt
       61 cttcctgttt cagaacatac agcttttttc gcagccgttt tacatgtttc attttaacag
      121 tatcaccgta aacaatctga ataccgtaaa gctgttcgca ttctgcaata agatctgcga
      181 ttttaatcaa taacttctcc tgattttttg ttactgcttt tttccacaca aatgtgtatt
      241 tattcgcaca ggtttcgatt tttgtgccgt ctataaaaat cgattctccg gaaatttctc
      301 caagctcata aagaagatta gacatttcag cgagaatccg tttcgcacac ggtgcaaaat
      361 gaatactgcg aaaacgagca aaagtggcat aatcaggaac tggtttacct tctaaaagat
      421 acataaagtt aatatcccgt ttacagttta attccatagc ctccacaaac tgactcagca
      481 accgcacaga atcgtctacc ggaataattg tttctaagtt taggtttcta agtttaaagg
      541 gagttttagt tgatatccct cctcatttaa agtataattc ttgtgtaata atataggggt
      601 ttgcatactt atattttaca ccaacagcca gacttttcaa agcctggctg taattttttg
      661 tatagaaaag aggccgcgca ctaattactt agtgcgacag cccctttttt tcgacatttc
      721 aactttttga agtgtctatt ttattttctt ttttttactc tgattacaga aacgcccgca
      781 aacattgcca ctgctatgaa catcatcata aatggtactg caaatggtgt tgcgtctcct
      841 gtctgaacag ttcccggttt cacttccggc gttgtcggtg tttccggtgt ctttccttct
      901 ttcgtcgtcg ctttcacaga tgctgccgct gatttatttc ctgctgcatc atatgctaca
      961 acttcgatcg tgtactctgt cttcggagta agttttgcga tctctgctgt aagaacatcg
     1021 tcttttactg ttccgattga cttgccgttt acaaagattt tgtatcctgt cactcctacg
     1081 ttgtctttag atgctttcca ttcaatcttc actgttgtat cagttttatc tgttactttc
     1141 agatcagatg gttttgtcgg agcttcttta tcttcttccg ggttcggatc tggtttctgc
     1201 tcttctttcg tcgtcgcttt cacagatgct ttctctgatt tatttcctgc tgcatcaaat
     1261 gcttctactt cgatcgtata ctctgtaccc gctttcagat ctttcagctc tgctgtaaga
     1321 acatcacctt ttactgtttc agcaagtttt ccgtctgcat atacgttata tcctgttact
     1381 cctacgttat ctgtagatgc tttccactca actttcacag ttgtcgtggt tgtctctgtt
     1441 tctttcaggt cagacggttt tgtcggagct tctttatctt cttccggctt cggcgaagtt
     1501 gcaagaagcg cttttcttgc atcctctaag tctttctctg ctttatctac tgctgcctga
     1561 tctgtcggat tttcaagaac tttctgcgca gccgcgtatg cagcttcaaa tgcagctttt
     1621 tcttttccat cttcaaatgc gtctaataca actttgtctg cattatttaa ttcagcttcc
     1681 aattttgttg tatcaacttc ccctgatttc accggagtga tcttcaggtt atcaagaatg
     1741 aggtcttgct gtccgtaagc tcctcccggc tgttttgcac cgtttgcata aataccgaac
     1801 catgtctgtc cgctctcgct tccggtaacc gtaaactcat gatgacttgc tttacctgtt
     1861 cctgccgtta ccgcaaggta atcattatct gttgcgttgt taaaacgctc tccatctcca
     1921 agcacaaccg catacgcaga tgatcctgcc tcataatcga attctacttt atatgtcttt
     1981 cctgcttcaa atcggaagtt ctgcggaatc gtatgataca aaagacctgt tcttcccgaa
     2041 ccgcttccac tgtcgcagtg ctgtttcagc gaccattcgc cctcaattac atcattgatc
     2101 acataaccgc cccaaccgac agatgtatat tttccgttat cctgagacag atgaagctgt
     2161 ccgtcgccgc ctctttgtgc cggtcctaaa acaaacggat aatatcctga tgttacggat
     2221 tcaaaatcct gctcgaaaga accgtcttct ttatagtttt caatcttctt gtcgataatt
     2281 ctgatatcgt caaagtatgt tgctccatca cctgccgcac ggctgatcgt caatgttgca
     2341 gtttcttttt ccgctgtaaa cggaacgtaa actctctgca tataggaagc ttcattgccg
     2401 cctgcatgtt tctgatcact tgccacatag ttctgagcga ttgatttcgt cgtatatgca
     2461 gactctttga tcgttcctgc atttacttct accgtcgctt tactgtcgct tctgttgtca
     2521 acataaactt ctgcaacata atctttgcct gctttgagtc ctgtcagttt tgtgctgact
     2581 gcaacgtctt ctgccgggct ttcgattgtc agacgctgat cgcctgtcca tgcttttgta
     2641 acttttacgg catcgttgtc tgctccgctc caaacgtctg catccacttt gtcgccgtct
     2701 gcataagagt tgaatcccgg atctttcact gatgtcgtct caccgaagtt aactgtatcg
     2761 gctgtcggca tttcttttcc gtcagtcacg attacgtaag ctgtttttgc ttttgcttca
     2821 agcgtgatct gtccgtttga aaccggaacc gcgatcgctt ctccacgtcc ctgatctgac
     2881 agctcataca ttgtaagatt tcctgtaaat ccgtccggca cattccatgt agatgttccg
     2941 ccttccagat tgtagtgata cagtttcgtc tctttcgtgt cgctatctgt ccacggaagc
     3001 agataataga aatgatcgtt tgtcgcactt aaaactgttt tatcattcaa tgtgatctcg
     3061 cgttcgatga tctcatcaga tctttgtgct tcatttcttg ttacaactac tttatcatca
     3121 ccgtttttca gtgtgatctg tttttcctgg tttccgaccg gtgactcgcc ttctgcgtag
     3181 ttctcccaat cgattacctg atagtgctga aggaattttg tcggaaggtt ttcggcaaat
     3241 gtcaggtaca gataattatt gaaatcctga tctccgcccc atccttcaaa tccttccagt
     3301 ttatatccgc cgagaagcgg gttgtcagcc gctccgccat gtcctaccca gttaagaacc
     3361 tgtacgtctc tcatgtcgtt tctgaggaaa cggatgatgt cactgttcaa accttttgta
     3421 gctgatccgc cgtatggtcc ttctgttgcc cagtgtgacc acgtggaatc atactcgcct
     3481 tcgtacggga actctgtgga gaatctccat ccgagagagt taatctcttc tgcgattctt
     3541 cttgtctccc acgcattctg ataccatacg tcaagataga tgaagtccat attgtcggcg
     3601 cctgcttttt tcactgctgc cgcaatctca tccgccgatg caagtgttcc ctgtgaatct
     3661 tttacatatt cgcctttatc ccagtcgaga cttaaaaggt tcgtattgtt aattctgtca
     3721 aataactgtg caaatctctt atatcttgcc tgtgttccta gatcccagat cttattgatc
     3781 acgcgggact ggtcaagcca tccccatccg ccctgctgcg gtcccatgat catatcatcg
     3841 ttgaaagatc tcgcttccgg ataagcttcc tgtgcattga catggatacc gatttctgta
     3901 tcatactcat gcgcaacgtc aagaagatct ctgaaatcat ccacgccgcc ttctctttcg
     3961 gagatatccg catactcgga gttcgcagag tcgtgacctt catttccgta acctttcagc
     4021 ataacagcct gcggaagtcc gtctgttgcc aaagcaactt tcttgatatt gtccgctgtc
     4081 accgaatacg ggtttgttac agcgcttccg aagttcataa cgattctgta gtttacaagg
     4141 tctttcacat cctctgatcc atacggaaca ttgatgatgt cacgataagc aactgcggca
     4201 tcctgccagt cgatcgtctt gtcttcgttc atatcctctg cgatacaaac ttttgcatat
     4261 ggaagttcac taagcgggta gtcatatgta gctgcctgtg cctgtccgcc tttatctccc
     4321 gcctcgtaat accactgagc gcttgtaagg ctcattgtgt cagctccgct gttcatgatg
     4381 actctcagat cgtcttctgc ctctgagttg ctgaacagac ctgcgcttaa attcttgttt
     4441 gtcaggaatc cgtatacata tcctacagat ttctgcgcta cgaatccatc gtcaaatgta
     4501 ataaacttat ctccgctctt ctttgtatcc gtgctcttaa cagcgcctgc aaatgaagcg
     4561 ttttcttcta cctgatctac agacagaaga ttcaactgcg gaatgtcgat agaagcaatc
     4621 ttatccgttc cttctttgcg gtcgatcttt gtgatctccc atgtaagatc attgtctttt
     4681 acactgatct
//
LOCUS       DBMDXF010000148         4635 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4476_length_4635_cov_1.477062, whole genome shotgun sequence.
ACCESSION   DBMDXF010000148 DBMDXF010000000
VERSION     DBMDXF010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_4635_cov_1.477062"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="ACZPD4_06500"
     CDS             <1..230
                     /locus_tag="ACZPD4_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1378746.1"
                     /translation="LNIADDYFKAKKQGDSLESDIELKDKEMYDLKHELISAQIKLEN
                     AEKELAKLKEENNDLQMQIVKLETEMKNRRR"
     gene            353..>1396
                     /locus_tag="ACZPD4_06505"
     CDS             353..>1396
                     /locus_tag="ACZPD4_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1378747.1"
                     /translation="MEKRRIEILAPAGSYASFKAAINAGADAVYAGGPKFGARAYADN
                     FTKEELIEAIKEAHIYGRKLYLTVNTLLKNNEISELPEYLSPLYEAGLDAVIVQDIGV
                     LELVREYFPKMDIHASTQMTITGYRGAAFMEGQGISRVVPARELSLEEVRMIKEKTGL
                     EIECFVHGALCYCYSGQCLLSSMIGGRSGNRGQCAQPCRLPYTVEGEKKYYLSPKDIC
                     TLDLIPELAEAGIDSFKIEGRMKKPEYVAAVTAMYRKYTDLYMQNGKKGYKVSSEDKR
                     ILMDLYNRGGFSAGYYRQHNGQNMIASDRPNHAGVPAVKVESQKGRLIKGKILTDLHA
                     GDVLDISGSYTIGK"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1407..2758
                     /locus_tag="ACZPD4_06510"
     CDS             <1407..2758
                     /locus_tag="ACZPD4_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HIR1378748.1"
                     /translation="KEQKKGEAFSMVVQKQVYIKQGSTINRVRNESLITSIHKQYIGD
                     SIRQKIDGKLTLEVGKPASLKLYCNESTYTAYSKEIVEQAQNRPMERERIVSQIEKLG
                     NTPFVFETLDIKMDEAIFLSIKTLNDLRREAVSGLCEALSGKYYRKTTDEKKEKRIAA
                     EVIEEKNNTYDQFKRKENFSYSVLVENKEQLSAVCAYLEKRETENIKIARIYAESNIF
                     QEDIPGYIEKLKKKKIEFYLALPHVFRKGSAERIEKCISRFSEKELDGVLIRNWEQYT
                     LLCQLQFDKKVISDDNLYVFNRFSKEFMKKAGFSEFTAPAELNESELKILGLETAELT
                     VYGYRPVMISAQCIMKTRGKCTKNSSFTQMKDRIGEEFLVQNRCDECYNIVYNSAPLY
                     LGTQKVKIQKLSPKRLRIRFGAERKEEVKKVLEQAIDAFGEKPQFDYTQEHFKRGVL"
     gene            2789..4261
                     /locus_tag="ACZPD4_06515"
     CDS             2789..4261
                     /locus_tag="ACZPD4_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1378749.1"
                     /translation="MVNIIIQLSKYVIIILMAAYTFSCFSIFTRNYEDEEKRVLIRQD
                     VLMFLLQFAAFGTMYLATKDIRILFLYAVLTVILLAVILLYNLIYPNVSRLVVNNMCM
                     LITVGMIMITRLSADNPSPYGTAVRQLVFVALGITFGLVVPVLIRKMKFLDQWTYIYA
                     AVGGAALLAVALFAQISGGAKLGFEIAGINIQPSEFVKIIFVFFVAASLNKSKEFKNI
                     VVTTAIAAAHVLILVLSTDLGAALIFFVVYLVMLFVATRQWLYAIAGLGAGAAAAVVG
                     YHLFSHIQVRVEAWQDPIGTYSGSGYQVAQSLFAIGTGSWFGTGLFKGQPDTIPVSET
                     DFIFSAITEEMGVIYALCLILICVSCYVMFLNIAMELRNNFYKLIALGLGTCYIFQVF
                     LQIGGVTKFIPSTGMTLPFVSYGGSSMLSTMIMFGIIQGLYIIREDEEEEREKEKLRK
                     ERKRKNGVGRTAPRKTAKSGPKFEEVPRQRPRKKTGTRTK"
     gene            4311..>4635
                     /locus_tag="ACZPD4_06520"
     CDS             4311..>4635
                     /locus_tag="ACZPD4_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR1378750.1"
                     /translation="MGYLVYFTTVKAKTFVNSPYNQRQDSFAQSVVRGDITDRNGNVL
                     ATTEVDEDGTETRTYPYGSLFSHVIGYNDSQFGKTGLESAQNFDLLTSNAFFMEKIQN
                     KFKGEK"
BASE COUNT         1527 a          717 c         1122 g         1259 t
ORIGIN      
        1 ctcttaacat tgccgatgat tacttcaaag caaaaaaaca gggtgattcg ctggagagtg
       61 acattgaact aaaagataag gaaatgtatg atttaaagca tgaacttatt tcagcgcaga
      121 tcaaactgga gaatgcggaa aaagagttgg caaaattaaa agaagagaac aacgatcttc
      181 aaatgcagat tgtgaagctc gagacagaaa tgaaaaatcg cagaagataa aaataaggct
      241 gtcagtcata tgacagcctg tttcttttgg atagtgtctt tttagatagt tgttgtttta
      301 tataaaggga aaacacctgt atgttaaagg ggatgtaggg aaaggagtca gcatggaaaa
      361 acgccggatt gaaatattgg cgcctgccgg atcttatgct tcttttaaag ctgcgatcaa
      421 cgcaggagcg gatgctgtgt acgccggagg tccgaaattt ggagcaagag catatgcgga
      481 taattttaca aaagaagagt tgatcgaagc gatcaaagaa gctcatatat acggaagaaa
      541 gctttacctt acggtaaata cactgctgaa aaacaacgaa atttcagagt tacccgaata
      601 tctgagtccg ctttatgaag cggggttaga tgcggtgatc gttcaggata taggagttct
      661 cgaacttgta agagagtact ttccgaagat ggatattcat gcgagtacgc agatgacgat
      721 cacagggtat cgcggagcgg catttatgga agggcaggga atcagccgtg ttgttccggc
      781 aagggaatta tctcttgaag aagtgcgtat gatcaaagaa aaaacagggt tggagataga
      841 atgttttgta catggtgcac tttgttactg ttattccggt caatgccttt taagcagcat
      901 gatcggcgga cgcagcggga acagaggtca gtgtgctcag ccttgcagac tgccgtatac
      961 agtagaaggt gaaaaaaaat attatttaag tccgaaagat atttgtacgc tggatctgat
     1021 cccggaactt gcggaggcgg gaatcgattc gttcaagatc gaaggtcgta tgaaaaagcc
     1081 ggagtatgta gcggcagtaa ctgcaatgta ccggaaatac acagatttat atatgcaaaa
     1141 cggaaaaaag ggatataaag tttcttccga ggataaacgt atactgatgg atctgtataa
     1201 cagaggagga ttttcggcag gttattatcg acagcataac gggcagaata tgatagcgtc
     1261 ggatcgtcca aaccacgcag gcgttccggc agtaaaggta gaaagccaaa aaggaagatt
     1321 gatcaaagga aaaattctta cagacctgca tgcaggggat gtgttggata tttcaggaag
     1381 ctatactatt ggaaaannnn nnnnnngaaa agaacagaaa aaaggggaag ctttttcaat
     1441 ggtagtgcag aaacaagttt atataaaaca gggatcgaca ataaacagag tgagaaacga
     1501 aagtcttatc acttcgattc ataagcagta tatcggagat tcaatcagac agaagataga
     1561 cggaaaactt acgctggaag tgggaaagcc ggcaagtttg aaattgtact gtaatgaaag
     1621 tacatatact gcgtactcaa aagaaattgt cgaacaggcg cagaaccgcc cgatggaaag
     1681 agaacggatc gtttctcaaa tagaaaaatt agggaatacg ccctttgttt ttgaaacttt
     1741 ggatattaag atggatgaag cgatttttct ctcgattaaa acacttaatg atcttcgcag
     1801 agaagcggta agcggattgt gcgaggcact atcggggaaa tattacagaa aaacaacaga
     1861 tgaaaaaaaa gaaaagagaa ttgcagctga ggttatagaa gaaaagaaca atacttatga
     1921 tcaatttaaa agaaaagaaa acttttctta ttcggttctc gtggaaaata aagaacagct
     1981 ttcggcagtc tgcgcctatt tggagaaaag agaaacggag aatataaaaa tcgcacgtat
     2041 ctacgccgaa agcaatattt ttcaggaaga tatcccggga tacatcgaaa aattaaaaaa
     2101 gaaaaagata gaattttatc ttgctcttcc gcatgtattc agaaaaggaa gcgcagaacg
     2161 gattgaaaaa tgtatcagtc ggttttcgga aaaagagtta gacggcgtac ttattaggaa
     2221 ttgggaacag tatacgctgc tttgtcaatt acaatttgac aaaaaagtaa tttcggatga
     2281 taatttatat gtgtttaatc ggttttcaaa agagtttatg aaaaaagcag gtttctctga
     2341 gtttacagcg ccggcagaat taaatgagag tgagcttaaa atacttggac ttgaaactgc
     2401 ggaactgaca gtatatggtt atcgcccggt tatgatttcg gcgcaatgta ttatgaagac
     2461 gagaggaaaa tgtacaaaaa actcttcctt tacgcagatg aaagacagga taggagaaga
     2521 gtttctcgtt caaaaccgat gtgatgaatg ttataatatc gtttataaca gcgctccttt
     2581 atatttgggc acacaaaaag taaagattca aaagctttca ccgaaaagac tgaggattcg
     2641 attcggagca gagcgaaagg aagaagtaaa gaaagtatta gaacaagcaa tcgatgcatt
     2701 tggagaaaaa ccgcagtttg attatacaca ggagcatttc aaacgcggcg tactgtaagt
     2761 gcgctcgatt atcgtcagga gaaataacgt ggtaaatatt atcattcaat tatctaaata
     2821 tgtaattatc atacttatgg ctgcttatac gttttcctgt ttctcgattt ttacgagaaa
     2881 ttatgaagat gaagaaaaaa gagtgcttat ccggcaggat gtattgatgt ttcttttgca
     2941 gtttgcagcg tttggtacaa tgtatttggc aacaaaagat attcggatct tgtttttata
     3001 tgcagtattg acggtgattc ttcttgcggt cattcttttg tataatttga tttatccgaa
     3061 tgtgtcaagg cttgttgtaa ataatatgtg tatgctgatc actgtcggga tgatcatgat
     3121 cacgagattg tccgcagata atccgtctcc ttacggcacg gcggtaagac agcttgtatt
     3181 tgttgctttg ggaattacct ttggacttgt tgttccggtt ttgatccgaa aaatgaagtt
     3241 ccttgatcag tggacatata tttatgcggc agtaggagga gcagctcttc ttgcagttgc
     3301 attgttcgca cagatttccg gcggggcaaa gctgggattc gaaattgcag gcataaatat
     3361 acagccgtct gagttcgtaa aaattatttt tgtatttttt gttgcagcaa gtctgaataa
     3421 atcaaaagaa tttaaaaata tcgttgtgac aactgcgatt gcggcagcac atgtattgat
     3481 cttagttctc tctacggatt tgggagcggc actgatcttc tttgttgttt atcttgttat
     3541 gctttttgtc gcgacaagac aatggctgta tgcgatagcg ggacttggag ccggagcagc
     3601 cgcggcagtt gtcggttatc atttgttttc gcatattcag gtcagggtag aggcgtggca
     3661 ggatcccatc gggacgtaca gcggaagcgg ttatcaagtg gcacaatcgt tgtttgcgat
     3721 cggtacggga agctggtttg ggacaggact ttttaaagga cagccggata cgatacctgt
     3781 ttcggagaca gattttatct tttctgctat tacagaagaa atgggagtga tttacgcact
     3841 ttgtctgatt ttgatctgtg taagctgtta tgtgatgttt ctgaatattg caatggaatt
     3901 gagaaataat ttttataagt taatcgcact tggattggga acgtgttata tttttcaggt
     3961 tttcctgcaa ataggaggcg tcacaaaatt tattccgtct acaggaatga ctttgccgtt
     4021 tgtaagttat ggcggaagtt cgatgttaag tacgatgatt atgtttggaa tcattcaggg
     4081 actatatatt ataagagaag atgaagaaga ggaacgggaa aaagaaaaac taagaaaaga
     4141 aaggaaaaga aaaaatggcg tgggaagaac agcgccccga aagacggcaa aaagcggacc
     4201 gaaattcgaa gaagtcccaa gacagaggcc gcggaaaaaa acgggaacgc gcacaaaata
     4261 aagatttcgc taaaattaca tatttctttg tgatcttgtt tattgcattg atggggtacc
     4321 tcgtttattt cacaactgta aaagccaaga cgtttgtcaa tagtccttat aatcaaaggc
     4381 aggattcttt tgcacaaagc gttgtcagag gagatattac agatagaaat ggcaatgtac
     4441 ttgcaacaac ggaagtggac gaagacggaa cagagacaag aacgtatccg tacggctcgc
     4501 tgttctccca tgttatcgga tataatgatt cacagtttgg aaaaacagga cttgagtccg
     4561 cgcagaattt tgatcttctt acttcaaatg cattttttat ggaaaagatt caaaataaat
     4621 ttaaaggaga aaaag
//
LOCUS       DBMDXF010000149         4614 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4500_length_4614_cov_2.381343, whole genome shotgun sequence.
ACCESSION   DBMDXF010000149 DBMDXF010000000
VERSION     DBMDXF010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_4614_cov_2.381343"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            205..1671
                     /gene="trpE"
                     /locus_tag="ACZPD4_06525"
     CDS             205..1671
                     /gene="trpE"
                     /locus_tag="ACZPD4_06525"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR1378751.1"
                     /translation="MNVLPTQEEVRRIASTGKYKVIPVSCEILSDFTTPIETLRRLKN
                     VSMRCYMLESAYANETRGRYTFLGFDPIMEIRCADGEMTAGILKLQTEHPSAYLRQIL
                     SEYKSPCFSYLPPFTGGLAGYFSYDYFGYSEASAKGQVEDTEKFPDVDLMLFDKVIAF
                     DHFRQKIILIVNMKLHDVESEYNKAVMELKQLAHLLKNGEKKKETGGKRQEKVTAAFG
                     KEQFCDMVQSAKKHIYEGDIFQIVLSNMLSAPYEGSLINAYRVLRTTNPSPYMFYFSG
                     TDLEVAGASPETLVKLENGTLHTFPMAGTRPRGRDETEDKALEKELLTDEKELAEHNM
                     LVDLGRNDLGKISRFGTVKVEKLHSVERFSHVMHIGSSVSGKIKEEYDALDAIEAVLP
                     AGTLSGAPKIRACQLIGELENNKRGIYGGAVGYIDFAGNMDTCIAIRMAYKKNGKVFV
                     RSGAGIVADSVPEREYEECLNKAKATLKALELAEEVEE"
     gene            1668..2276
                     /locus_tag="ACZPD4_06530"
     CDS             1668..2276
                     /locus_tag="ACZPD4_06530"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR1378752.1"
                     /translation="MILLIDNYDSFSYNLYQMIGEIEPDVKVVRNDEISVDEIRRLKP
                     ERIILSPGPGRPEDAGILIEVVKELGKEIPILGVCLGHQAVCAAFGAKITYAKKQMHG
                     KQSQIKVDTETLLFSGCSQSILAARYHSLAADADTIPDSLYVTASTKDGEIMAIQHKE
                     YPIYSVQFHPESIMTPEGRQILRNFIQGGIRYDQRSDRKNCK"
     gene            2242..3273
                     /gene="trpD"
                     /locus_tag="ACZPD4_06535"
     CDS             2242..3273
                     /gene="trpD"
                     /locus_tag="ACZPD4_06535"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR1378753.1"
                     /translation="MIKEAIVKIVNKEDLTYDEAYTVMNEIMSGQTSETQNAAFLAAL
                     STKSARAETTDEIAGCAAAMRAHATRVETGMELFEIVGTGGDHAQSFNISTTAALVAA
                     AAGMKVAKHGNRAASSQCGTADCLEALGVNIHQNPKKCLELLNEAGMCFFFAQEYHTS
                     MKYVGAIRKELGFRTVFNILGPLTNPGTPTMQLLGVYDEYLVEPLAQVLLDLGIRRGM
                     VVYGQDKLDEISMSAATTVCEIRDGWFRTFVLTPEEFGFERCSKEDLKGGTPKENAEI
                     TLAILKGEKGPKRNAVLLNAGAALYIGEKADSIKAGIALAEELIDSGKALDTLQKLIE
                     GSNRGEGCV"
     gene            3270..4052
                     /gene="trpC"
                     /locus_tag="ACZPD4_06540"
     CDS             3270..4052
                     /gene="trpC"
                     /locus_tag="ACZPD4_06540"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR1378754.1"
                     /translation="MTILEQLAGYARERTEKAKQKRPLSTLRQQAFSMPKGSFAFENA
                     IKKPELSFICECKKASPSRGIIVSDFPYIQIAKEYENAGADCISVLTEPGWFLGKDAY
                     LREIAEVVYIPCLRKDFTVDEYMLYEAKVLGASAVLLICSILSERQIQEYIGICDEMG
                     ISALVEAHDEKEVTMAVRAGARLIGINNRNLKDFTVDTDNSRRFREMIPPEILFVSES
                     GICSAEDVKRLREIGTDAVLIGEALMRTENKAEKLAWLRGAI"
     gene            4049..4543
                     /locus_tag="ACZPD4_06545"
     CDS             4049..4543
                     /locus_tag="ACZPD4_06545"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012900.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR1378755.1"
                     /translation="MTKIKFCGLSRRCDIEAVNELKPEYIGFVFVKTSKRYVSFEKAE
                     ELKSLLDPKIKAVGVFVNEDLKRIEEICDKKVIDIVQLHGLEEEKELNYLRSRIVQPV
                     IKAFCIKKEEDVRQAERSPADFILLDSGAGTGRTFEWEWIKHMERPYFLAGGIHIDNV
                     EKAE"
BASE COUNT         1454 a          814 c         1214 g         1132 t
ORIGIN      
        1 gttggtatcc tgctaagtgc acagggaatc ctgtgaatgc aaggtggcac cgcggatagt
       61 gtcgtacttt ttatttatgt acacgattcg tccttgacag atttattttt ttctgtcagg
      121 ggcgtttttt ttattcccat aaattcctct gacaagattg caacgaaaag tagtgtaatg
      181 atttctggat cggaggtatg aagtatgaat gttttaccga cacaagaaga agtcagaaga
      241 atcgcttcaa ccggaaaata taaagtgata ccggtcagct gcgaaatact ttctgatttc
      301 acaactccta tcgaaacgtt aaggagattg aaaaatgtat ctatgcgctg ttatatgctg
      361 gaatctgcat atgcaaatga aacaagggga cgttacacgt ttctcggttt tgatcccatt
      421 atggagatac gttgtgcgga cggagagatg accgccggaa tactgaagtt acaaacagag
      481 catccgtcag cgtatttacg ccagattctc tctgaataca aaagtccttg cttttcttat
      541 cttccgccgt ttaccggagg tcttgcggga tatttttcct acgattattt tggatatagc
      601 gaagcgtcgg caaaagggca agtggaagat acagaaaaat ttccggatgt agatttgatg
      661 ctgtttgaca aagtaatagc gttcgatcat ttcagacaga agatcattct tattgtcaat
      721 atgaaactgc atgatgttga aagtgaatac aataaagcgg tcatggaact aaaacagctt
      781 gctcatctgt taaaaaacgg ggaaaagaag aaagaaaccg gcggaaagcg tcaggaaaaa
      841 gttacggcgg cgtttggaaa agaacagttt tgcgatatgg ttcaaagcgc aaaaaaacat
      901 atttatgaag gagatatttt tcagatcgta ttatcgaata tgttatcagc gccttatgag
      961 ggaagcctca tcaatgctta tcgggttctt aggacaacga atccttctcc gtatatgttt
     1021 tatttttcag gaacagatct tgaggtggca ggcgcttcac cggaaacatt ggttaaattg
     1081 gagaacggaa cgcttcatac gtttccaatg gcaggaacga gaccaagagg aagagatgag
     1141 acggaagata aagcgctgga aaaagaactg ctgacagatg aaaaagagtt ggcagagcac
     1201 aacatgctag tagatctggg gagaaacgat cttgggaaaa tcagccggtt cggcactgtg
     1261 aaagtagaaa aattacattc tgtggagcgg ttttcccatg tcatgcatat aggctcttcg
     1321 gtgagcggga aaataaaaga ggagtacgat gctttggatg ccatagaagc ggtactgccg
     1381 gcaggaacac tttcaggagc gccgaagatc cgggcttgtc agctgatcgg cgaactggag
     1441 aacaacaaac ggggcattta tggcggcgcc gtcggttata ttgattttgc gggaaatatg
     1501 gatacctgta ttgcaatccg catggcatat aagaaaaacg gaaaggtttt tgtaagaagc
     1561 ggagccggga tcgtagcaga ttccgtaccg gaacgagaat atgaagagtg tttaaataaa
     1621 gcaaaagcta cattgaaagc gctggaactt gcagaggagg tggaagaatg attttactca
     1681 ttgataatta tgacagcttt tcctataacc tttatcagat gatcggcgag atagaaccgg
     1741 atgtgaaggt tgtccgtaat gatgaaatat ctgtggatga aatccgacgt ctgaagccgg
     1801 agaggatcat tctttcaccc ggacccggca gaccggaaga tgcagggata ctcatagaag
     1861 ttgtaaaaga acttgggaaa gaaattccaa tcctcggtgt ctgcctggga catcaggcag
     1921 tctgcgcggc ctttggagcg aaaattactt atgcaaaaaa gcaaatgcac ggaaagcagt
     1981 ctcagataaa agtcgatacc gaaacgttgt tgttttcagg ctgctcacag agcattttgg
     2041 ctgcaagata tcattctctt gcggcagacg ccgacacgat tccggacagt ctctatgtta
     2101 ccgcctccac aaaagacgga gaaatcatgg cgatacagca taaggaatat ccgatttaca
     2161 gtgtgcagtt ccatccggaa tccattatga caccggaggg aagacaaata cttagaaatt
     2221 ttatacaagg aggaataaga tatgatcaaa gaagcgatcg taaaaattgt aaataaggaa
     2281 gatctcacat atgacgaggc atatacagtt atgaatgaaa ttatgagcgg acagacatcc
     2341 gagacgcaga atgcggcttt ccttgctgca ctgtcaacca aaagcgccag agcagagacg
     2401 acagatgaaa ttgccggatg tgcggctgcc atgagagcac atgcgactcg tgtggagacc
     2461 ggaatggaat tgtttgaaat cgtaggaaca ggcggggatc atgcacagag ttttaatatt
     2521 tcgactacag cggcattggt ggcagcggcg gcaggaatga aggttgccaa gcacggaaat
     2581 cgggcggcat cctcccaatg cggaacggca gactgtctgg aagctctcgg agttaatatt
     2641 caccagaatc cgaaaaagtg tctggaactg ttaaatgagg caggaatgtg ctttttcttt
     2701 gcacaggagt atcatacttc catgaaatat gtgggggcaa tacgaaaaga attaggtttt
     2761 cgtaccgttt tcaatatttt aggcccgttg acaaacccgg gaacacctac gatgcagctg
     2821 ctgggagtat atgacgagta tctcgtggaa cctttggcgc aggtattgct cgatctcggc
     2881 atccgacggg ggatggtagt atacggacag gataagctgg atgaaatttc tatgagtgca
     2941 gcgacgaccg tatgtgagat tcgggacgga tggtttcgga cttttgttct cacaccggaa
     3001 gaattcgggt ttgaacgctg cagtaaagaa gatttaaaag gcggcacacc gaaagaaaat
     3061 gcagagatca cgcttgcaat cttaaaaggg gagaaaggtc ctaagagaaa tgccgtattg
     3121 ctcaatgccg gagcggcgct ttatatcggc gaaaaagcgg acagtataaa ggcgggaatt
     3181 gcactggcag aagaacttat tgattcagga aaagcattgg atacgctgca aaagctgatc
     3241 gaaggaagca atcgtgggga ggggtgtgta tgacgatttt agaacagctt gccggctatg
     3301 ccagagaacg aaccgaaaaa gcaaaacaga aacgcccgct ttcgacgttg aggcaacagg
     3361 ccttttcaat gccgaaagga agttttgcat ttgaaaatgc gataaaaaag ccggagcttt
     3421 cttttatctg tgaatgtaaa aaggcgtctc catccagagg gatcatcgtt agtgattttc
     3481 catatataca gattgcaaaa gagtatgaga acgccggagc agactgtatt tccgtattga
     3541 cagaaccagg atggttttta gggaaggatg catatcttag agaaatcgcc gaagtagtat
     3601 atattccttg tctgcgaaaa gattttaccg tagatgaata tatgctctat gaagcaaaag
     3661 tgttgggggc atcagcggta cttctgatct gttctatatt aagtgagcgg cagattcagg
     3721 aatatatcgg tatctgtgat gagatgggga tttcggcttt ggtagaggca catgatgaaa
     3781 aagaagtgac aatggcggtt cgtgccgggg cgcgtcttat aggaatcaac aatcggaatt
     3841 taaaagattt tacagtagat acagacaata gccgcaggtt tcgtgaaatg attccgccgg
     3901 agatactgtt tgtttcggaa agtggaattt gcagcgccga agatgtgaaa agattgcgtg
     3961 agatcggcac agatgcggtg ctgatcggag aagcgcttat gagaacagaa aacaaggcgg
     4021 aaaagctggc ttggctaaga ggtgctatat gacaaaaata aaattttgcg gtttgtcccg
     4081 tcggtgtgat attgaagcgg taaacgaact gaaaccggaa tatatcggat tcgtatttgt
     4141 aaagacaagt aaaagatatg tttcctttga aaaagcggag gaattaaaaa gcctgctgga
     4201 tccaaagatc aaggcggtcg gagtttttgt aaatgaagat ttaaaacgaa tcgaagaaat
     4261 ttgcgataaa aaggtgatcg acatcgttca gcttcatggc ctagaagaag agaaggaact
     4321 gaactatctg agaagccgta ttgttcagcc ggtcatcaaa gctttttgta taaaaaagga
     4381 agaagatgtc agacaagcgg aaagaagtcc ggcggatttt attttattgg attccggtgc
     4441 cggaaccgga agaacttttg aatgggagtg gataaaacat atggaaagac cgtattttct
     4501 tgcaggagga atccatatag ataatgtaga aaaagctgaa taataaaatt aagagtctga
     4561 aaaaagccat cgaggacagc gccgcccgac aggaaaaagc cagacagccg gaaa
//
LOCUS       DBMDXF010000150         4483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4662_length_4483_cov_1.550662, whole genome shotgun sequence.
ACCESSION   DBMDXF010000150 DBMDXF010000000
VERSION     DBMDXF010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4662_length_4483_cov_1.550662"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="alr"
                     /locus_tag="ACZPD4_06550"
     CDS             complement(<1..803)
                     /gene="alr"
                     /locus_tag="ACZPD4_06550"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1378756.1"
                     /translation="MDQHNRVCAQIDLDAIAYNMEQMKDQIGKEARLIAVIKTDGYGH
                     GAVPIARMFETFSYVWGYAVACIEEAVSLRKEGIKKPILVLGCVFPDQYETMIKNEIR
                     AAVYTKEMAEGMSDEAVRQGKNAYFHIKIDTGMGRIGFPVNSESVNLIEEISKLPNIH
                     MEGLFTHFAKADEKEKSYTLKQHERFLWMKEQLEKRNVDITYYDCDNSAGIIDFPNLK
                     HDLARAGISTYGMYPSDEVQKSNVHLKPALSLISHITFVKEVEAGTPIS"
     gene            complement(848..2389)
                     /locus_tag="ACZPD4_06555"
     CDS             complement(848..2389)
                     /locus_tag="ACZPD4_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HIR1378757.1"
                     /translation="MRYLPNGAQMKAADTYTIEKVGIPSLVLMERAALETVRVLKDRG
                     VDFSDTLVVCGSGNNGGDGFAAARILKEYGFCAEVLFVGQDASMSEECRTQKQIAERL
                     GISVFTDFPKKEYTVIIDAVFGVGLSRAIEGRYHTVIEWMNDKKCEKAAIDIPSGICA
                     ESGRVLGIAFRADITVSMECVKLGCELFPGKLYAGETVSVPIGIDLSFFEKNKDVCIT
                     YDPEDIPLLLPKRAADSHKGDYGKILMITGSKGMAGAAYLSAKAAYAVGAGLVQIYTA
                     EENRTVLQELLPEAIISCYNETAGKPDDKELEKLIQWADVICIGCGLGTSVFASRLLK
                     KTMEILRENGSEEEKLRSCPCIIDADGLNLLSMDMEQLQGVPNVILTPHMKEMSRLIN
                     KEIPQIAERRFSVVKEFTEQYQVVCALKDSRTVVMKEGHHPFLNLAGNSAMAKAGSGD
                     VLAGMISGLAAQKTDIFDAVSAGVYFHACGGDEARKKKGSYSVLARDLIDGIGQCLKN
                     AEERL"
     gene            complement(2464..3108)
                     /locus_tag="ACZPD4_06560"
     CDS             complement(2464..3108)
                     /locus_tag="ACZPD4_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1378758.1"
                     /translation="MERRKISRAVISRLPRYYRYLGDLLEIGVERISSNDLSKKMNVT
                     ASQIRQDLNNFGGFGQQGYGYNVKYLYTEIGKILGLDKTHNFIIIGAGNLGQALANYA
                     SFERSGFVLKSLFDVNPRFEGMAIRGIEVRMMDELVDFLKNNDIEIAALTIPKSKAIE
                     VADLLVANGIKAIWNFAHTDLNLPKDVIVESVHLSDSLMKLSYNISQREGGQKK"
     gene            3313..>4483
                     /locus_tag="ACZPD4_06565"
     CDS             3313..>4483
                     /locus_tag="ACZPD4_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1378759.1"
                     /translation="MILACHNLKKSFGDRIIVDTGSFHIEEREKAALVGVNGAGKSTI
                     FKMIIGEEPSDGGEIILAKGRSIGYLAQHQNLIPNGTIYEELRSAKADIIEMEQQLRS
                     IEHELQSLSGEELNARLETYNRLQSQFESQNGYACESEIIGVLKGLGFTEEEFSKKTD
                     TLSGGQKTRVALGKLLLTDPDILLLDEPTNHLDLNSISWLETYLMNYNGAVFIVSHDR
                     FFLNRVVTKVIEIENGALSMYMGNYRAYSEKKQQIRDAKLKEYFNQQREIKHQQAVIE
                     KLRSFNREKSIRRAESREKLLEKITIVDKPQEAVREMHFSLEPYCVSGNDVLTVEHLS
                     KQFDTQLLFQDVSFEIKRGEHVAVIGDNGTGKTTLLKILNQVIKADNGSFTLGAKV"
BASE COUNT         1271 a         1125 c          745 g         1342 t
ORIGIN      
        1 tagctaatcg gtgttcctgc ctctacttct ttcacaaatg taatatgact gatcaatgaa
       61 agagccggtt tcagatgaac attgctcttt tgcacttcgt ctgacggata cattccatat
      121 gtagaaatgc ctgctcttgc aagatcatgc ttcaaattag gaaaatcaat gattccggcg
      181 ctgttatcac agtcataata cgtaatatct acattccgtt tttccaactg ttccttcatc
      241 cacaaaaaac gttcatgctg ctttaacgta tatgattttt ctttttcatc tgctttggca
      301 aaatgagtaa ataacccttc catatgtata ttcggaagtt tactgatctc ctcgatcaaa
      361 tttacacttt cgctatttac aggaaatcct atccgtccca ttccggtatc gatcttaatg
      421 tggaaataag catttttccc ctggcgaacc gcttcgtctg acatcccttc cgccatctct
      481 tttgtgtaga cagccgcacg gatctcgttt tttatcatcg tttcatattg atccggaaaa
      541 acacagccaa gtacaaggat cggtttttta attccttctt tccgaagaga caccgcttct
      601 tcaatacagg cgacggcata cccccataca tatgaaaatg tttcaaacat ccgcgcaatc
      661 ggaacagcgc catgcccata tccgtccgtc ttgattacag cgatcagacg cgcctctttt
      721 ccgatttgat ctttcatctg ttccatatta taagcgattg cgtccagatc aatctgcgcg
      781 catacccgat tatgttgatc catgtcctta ctccttatat cactcatctg tttctctttt
      841 tgatctgtca aagcctttct tccgcattct tcaaacattg ccctatccca tcgatcagat
      901 ctcttgctaa aacactgtaa ctccctttct tttttcttgc ttcatctccg ccgcatgcat
      961 gaaaatatac ccctgccgat acagcgtcga aaatatctgt tttctgagca gcaagccctg
     1021 agatcattcc ggcaagaaca tcccccgatc ctgcttttgc catggcgctg tttcccgcga
     1081 gattcaaaaa aggatgatgt ccttccttca taacgacagt tctgctgtct tttaacgcac
     1141 atacaacctg atattgttcc gtaaattctt tcacgacaga aaaacgacgt tctgcaatct
     1201 gagggatctc tttattgatc aatcttgaca tttctttcat atgcggagtc aaaatcacat
     1261 tgggaactcc ctgcaactgt tccatatcca tactgagaag atttagtccg tctgcatcga
     1321 taatacacgg acaagatcgg agtttttctt cttcactgcc attttctctt aatatttcca
     1381 tcgtcttttt caaaagccgg ctcgcaaaca cacttgttcc aagtccgcat ccgatgcata
     1441 taacatctgc ccattgaatc aatttttcca gttctttatc atcaggcttt ccggccgttt
     1501 cgttatagca cgatataatt gcttcaggga gcaattcctg taaaacagtc ctgttttctt
     1561 ccgccgtata aatctgaaca agtccggcgc cgaccgcata tgcggccttt gccgacaaat
     1621 acgcggcgcc cgccattcct ttactcccgg taatcattaa aatttttcca taatctcctt
     1681 tgtgagaatc cgctgctcgt tttggcaaaa gcaaaggaat atcttccgga tcatatgtaa
     1741 tacatacatc tttgtttttt tcaaaaaaag aaagatcgat tccgatcgga actgatactg
     1801 tttccccggc atacagtttc cccggaaata attcgcatcc caacttcaca cactccatag
     1861 aaactgtaat atctgcacga aatgcaattc ccaaaactct gccgctctcc gcacatatgc
     1921 cggatggaat gtcgatcgct gctttttcac acttcttatc attcatccat tcaattacag
     1981 tatggtacct gccctcaata gccctgctca atcctacgcc aaacacagcg tcaattataa
     2041 cagtatattc ctttttagga aaatctgtga acacagaaat gcccagccgt tctgcaattt
     2101 gcttctgcgt ccggcattcc tcgctcatag acgcatcttg ccctacaaat aatacttctg
     2161 cacaaaaacc atattccttc aatatacgtg cagccgcaaa tccgtctccg ccgttatttc
     2221 cgcttccaca tacaacaagt gtatctgaaa aatctactcc tctgtctttc aaaactctaa
     2281 ctgtttcaag cgcagccctt tccatcaata cgagtgacgg aatccctact ttttcaatcg
     2341 tatatgtatc tgccgctttc atctgcgctc cattgggaag atatctcatt gatctcaccg
     2401 cctttattat gctgtttaaa tgggaacgcc cgcagtttcc tgcggggttc tgtcttcaag
     2461 tatctatttt ttctgtccgc cttctcgctg actgatatta taagacaatt tcataagact
     2521 gtctgaaaga tgtacacttt ccacaatcac atctttcgga aggtttaaat ctgtatgtgc
     2581 aaaattccat atagccttga ttccattcgc aacgagaaga tccgcaactt caatcgcttt
     2641 tgacttcgga atcgtcagtg cggcgatctc tatgtcatta tttttcaaaa aatctaccag
     2701 ttcatccatc atgcgcactt cgattccccg aattgccatt ccctcaaaac gtggatttac
     2761 atcgaacaga cttttcaaaa caaagccgct cctctcaaaa gaagcataat ttgcgagcgc
     2821 ctgtcccaaa tttcccgctc cgataataat aaaattatgc gttttatcaa gtcccaatat
     2881 cttgccgatc tctgtataaa gatacttcac attatatcca tacccctgct gtccgaatcc
     2941 gccgaaattg tttaaatctt gacgaatctg agacgccgtt acattcatct tcttactcag
     3001 atcattggaa gagatcctct cgaccccgat ttcaagcaga tctcccaaat accgataata
     3061 tctcggcagt cttgaaatta ccgctctgga tatttttcta cgctccacgt ttatcacctt
     3121 tccaccgcgc cgtctatact cgcgcttgta tctatattgt ttcacacgtc ggacgtgccg
     3181 acacttccgt tccattatat aaaatagtta atattatgtc aatttttact ttttatttac
     3241 aggacattca gctataatag attggaaaac agaccattac tataatcgtt attatgaaag
     3301 aggaattata tcatgatact tgcatgtcat aatcttaaaa aatcattcgg agaccgaatc
     3361 attgtcgata ccggatcttt tcatatagag gaacgtgaaa aagcagctct tgtcggggta
     3421 aacggcgccg gaaaatcaac aatcttcaaa atgatcatcg gagaagaacc ttctgacgga
     3481 ggagaaataa tccttgcaaa aggccgctca atcggttatc ttgcccaaca tcaaaacctg
     3541 attccaaacg gtaccattta cgaagaactt cgatctgcca aagcagacat catcgaaatg
     3601 gaacaacagc ttcgctcaat cgaacacgaa ctacaatcat tatccggaga ggaattaaat
     3661 gcccgtctcg aaacatacaa ccgccttcag tcgcaatttg aatctcaaaa cggatatgcg
     3721 tgtgaaagtg agattatcgg tgtgttaaaa ggacttggat ttaccgaaga agaattttca
     3781 aaaaaaacag atactttatc cggtggacag aaaacccgtg tcgccctcgg gaaacttctt
     3841 cttaccgatc ccgacatcct gctcctcgac gagcctacaa accatttaga tttaaattcg
     3901 atctcatggc ttgaaactta tttaatgaac tataacggcg ctgtattcat cgtatctcac
     3961 gatcgctttt tcttgaatcg agtagttaca aaagtaattg aaattgaaaa cggcgctctt
     4021 tctatgtata tgggaaatta ccgcgcctac tctgaaaaaa aacagcagat ccgtgatgcg
     4081 aaactaaaag aatatttcaa tcagcagcgt gaaatcaaac atcaacaggc agtaatcgaa
     4141 aaacttcgct ctttcaatag ggaaaaatcc atccgccggg cagaaagccg tgaaaaatta
     4201 ttagaaaaaa tcaccattgt agataaacct caagaggcag tccgggaaat gcatttttct
     4261 ctggaaccgt actgtgtcag cggaaacgat gtactgacag ttgagcatct ttcaaaacag
     4321 tttgataccc aactcctttt tcaagatgtc agttttgaga tcaaacgcgg ggagcatgta
     4381 gctgtcatag gagataacgg aacaggaaag acaacgcttc taaagatatt aaatcaagtg
     4441 atcaaagccg acaatggctc tttcactctc ggagccaaag tac
//
LOCUS       DBMDXF010000151         4468 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4675_length_4468_cov_1.455919, whole genome shotgun sequence.
ACCESSION   DBMDXF010000151 DBMDXF010000000
VERSION     DBMDXF010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4675_length_4468_cov_1.455919"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="ACZPD4_06570"
     CDS             complement(<1..244)
                     /locus_tag="ACZPD4_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378760.1"
                     /translation="MYEGNPVDLRMEKIISADGIFDEATRQCRVEKYDPEEDYIYLEL
                     KEDELTVISLDAKYRCYISTRTELLYCKGVVKERYCR"
     gene            complement(293..1072)
                     /gene="codY"
                     /locus_tag="ACZPD4_06575"
     CDS             complement(293..1072)
                     /gene="codY"
                     /locus_tag="ACZPD4_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIR1378761.1"
                     /translation="MSVQLLDKTRKINKLLHNNNSSKVVFNDICAVLTEILDSNVLVV
                     SRKGKILGVSKSPDVQEISELITEAVGSHIDQMLNERLLSILSTKENVNLETLGFSAE
                     AVQGCQAIVTPIDIAGERLGTLFIYKQDKTYSIDDIILSEYGTAVVGLEMLRSVNEES
                     AEETRKEHIVQSAISTLSFSELEAIIHIFDELGGTEGILVASKVADRVGITRSVIVNA
                     LRKFESAGVIESRSSGMKGTYIKVLNDYIFEELEKIKKERM"
     gene            complement(1254..>3308)
                     /gene="topA"
                     /locus_tag="ACZPD4_06580"
     CDS             complement(1254..>3308)
                     /gene="topA"
                     /locus_tag="ACZPD4_06580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1378762.1"
                     /translation="VIVESPAKVKTIKKFLGSNYTVAASQGHVRDLPKSSLGIDVEHD
                     YEPKYITIRGKGDILAALRKEAKKSDKVFLATDPDREGEAISWHLATALKLDEKKMRR
                     ITFNEITKNAVKASLKAPRDIDMNLVDAQQARRVLDRMVGYRISPLLWAKVKRGLSAG
                     RVQSVALRLVADREEEINAFIPEEYWTLDVILKIKGEKRPLTAKFYGTDQKKMTIRSE
                     QELNEIVKKIENAEFKVSDIKTSERFRKAPLPFTTSTLQQEAAKALNFGTQKTMRIAQ
                     QLYEGVDIKGGGTVGVITYLRTDSTRIADEADASAREYIASVYGEKYIAAGQTKQAEG
                     KKIQDAHEAIRPTDISRTPASIKESLTRDQFRLYQLIWKRFAASRMMPAQYESTSVKI
                     AAGQYRFSVSTSRCVFDGFRSVYTEADEEKEESNVLVGHLSMDSVFTREDFDTKQHFT
                     QPPAHYTEASLVKALEELGIGRPSTYAPTISIILGRRYVTKEAKNLYLTEIGEVVNNI
                     MKQSFPSIVDANFTANMEGLLDMVEEGKVAWKDVIRNFYPDLDEAVKRAEIELESVKI
                     EDEVTDVICEECGRNMVIKYGPHGKFLACPGFPECRNTKPYLEKIGVPCPVCGKDVVI
                     RKTKKGRKYYGCENNPECDFMSWQKPSKEKCPDCGGYMLEKGNKLVCADEKCGYVKTI
                     GK"
     assembly_gap    3309..3318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3439..4468)
                     /gene="dprA"
                     /locus_tag="ACZPD4_06585"
                     /pseudo
     CDS             complement(3439..4468)
                     /gene="dprA"
                     /locus_tag="ACZPD4_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847175.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
BASE COUNT         1141 a         1164 c          684 g         1469 t
ORIGIN      
        1 cgcgacaata tcgttctttc acaacccctt tgcaatacaa aagttctgtc cttgtcgaaa
       61 tataacatct gtatttagca tcgagtgaaa ttaccgtcaa ttcatcttct ttcaactcaa
      121 gataaatgta atcttcctcc ggatcatatt tttcaaccct gcactgcctt gtcgcttcat
      181 caaaaattcc atctgccgat atgatttttt ccatacgcag atctacggga tttccttcgt
      241 acatcgttct tcctcctgcc tgcctctatc tgactttccg ctttttaaaa aactacatac
      301 gttctttttt aattttctca agttcttcaa aaatatagtc gttcaacact tttatatagg
      361 ttcccttcat accggaagaa cgagattcaa ttactcccgc actttcaaac ttacgcagcg
      421 cattcacaat aaccgaacgt gtaatgccta ctctgtcagc tactttactt gcgacaagaa
      481 taccttccgt tccgccgagc tcatcaaaaa tgtgaataat cgcttctaac tccgaaaatg
      541 acagtgtact gatcgccgat tgcacgatat gttcttttct tgtttcttcc gcactttctt
      601 catttacaga acgaagcatc tccaaaccga caacagcagt tccgtattcg ctcaaaataa
      661 tgtcgtctat cgaatacgtc ttatcttgct tataaataaa taatgttcca agcctctctc
      721 ccgcaatatc aatcggagtt acgattgcct ggcacccctg caccgcctct gctgaaaatc
      781 caagtgtctc cagattcaca ttttcttttg tagaaagaat gcttaaaagt ctttcattta
      841 acatctgatc gatatgagat ccgacagcct ctgtgatcaa ttcactgatt tcctgaacat
      901 ccgggctttt acttacgccg agaatcttcc cctttctgct taccacaaga acatttgaat
      961 caagaatttc ggtcaatacc gcacaaatat cattaaatac gaccttactt gaattattgt
     1021 tatgaagcaa tttattaatt tttcttgttt tgtctaacag ttgtacgctc attatttcct
     1081 ccgtttgctt cttacaagcc aataattgta tacacaaatc cagataaata tatagataat
     1141 aatatgctat cacacaattt ttctgatttc aatacatttc cgcagtttta tttcttattt
     1201 tttatttttt ttatttataa tccgaatgcc tctgtttttg tttggtttcc actctatttc
     1261 ccgattgttt ttacatatcc gcatttttca tctgcacata cgagtttatt tcctttttcc
     1321 aacatgtaac cgccgcagtc aggacacttt tctttcgacg gcttctgcca cgacatgaaa
     1381 tcacactccg gattattttc acatccgtaa tattttctgc ctttctttgt cttacgaatc
     1441 acaacgtcct ttccgcatac cggacaaggt acgcctatct tttcaaggta tggtttcgta
     1501 tttctgcatt ccggaaaccc ggggcatgcc aaaaattttc catgaggccc gtacttgatt
     1561 accatgtttc ttccacattc ttcgcagatc acatctgtca cttcatcttc gatcttgaca
     1621 ctttcaagct cgatctccgc acgttttacc gcctcatcca gatccggata aaaattacgg
     1681 atcacatctt tccatgcaac ctttccttcc tcaaccatat ctaaaaggcc ttccatattt
     1741 gccgtgaaat ttgcatccac aatacttggg aaagattgtt tcataatatt gttcaccact
     1801 tccccaattt ccgtcaaata taaatttttc gcctcttttg tcacatatct gcgcccaagt
     1861 atgattgaaa tcgtaggggc atacgtactc ggacgcccga ttccaagttc ctccaacgcc
     1921 tttacaagac tggcttctgt ataatgcgcc ggcggctgtg taaaatgctg ttttgtatca
     1981 aaatcttctc ttgtaaatac agaatccata cttaagtgtc ctacaagaac attactttcc
     2041 tctttttctt catcagcttc tgtatagaca gatctaaacc cgtcaaatac acatcttgat
     2101 gtcgaaaccg aaaaacgata ctgcccggct gcaatcttaa ccgaagtaga ttcatattgc
     2161 gccggcatca tacggcttgc cgcaaaacgt ttccatatca gttgatacaa acggaactga
     2221 tctcttgtga gagattcttt gatcgaagcc ggagttctgc tgatatccgt cggacggatc
     2281 gcctcatgtg catcctgtat tttctttcct tccgcctgct tcgtctgccc cgccgcaata
     2341 tacttttctc cgtaaacaga agcgatatat tctcttgccg atgcgtccgc ttcgtctgcg
     2401 attcgagtag aatccgtacg aagataagtg atgacaccga ctgttccgcc gcctttaata
     2461 tcaacacctt cataaagctg ctgcgcaatg cgcatcgtct tttgagttcc gaaattcaga
     2521 gccttcgccg cttcctgctg cagtgtactc gtagtaaacg gaagaggagc ttttctgaac
     2581 cgctcgctcg tttttatatc tgaaactttg aattctgcat tctcgatctt tttgacaatc
     2641 tcatttaatt cctgctctga tctgatcgtc atttttttct gatctgttcc gtaaaacttt
     2701 gctgtaagag ggcgcttttc cccttttatc ttcaaaatca cgtcaagtgt ccaatactct
     2761 tccggaataa acgcattaat ctcctcttct ctgtctgcca caagacgaag cgctaccgac
     2821 tgcacacgcc ccgcgctcag tccgcgtttt acttttgccc aaagaagcgg gctgatccgg
     2881 tatccgacca tacgatccag aacgcggcgt gcctgctgcg catcaactaa attcatatca
     2941 atatcccgcg gagctttcaa ggatgccttc actgcatttt ttgtaatctc attaaaggta
     3001 atcctacgca tcttcttttc atctaatttc agcgcagtcg caagatgcca cgaaatcgcc
     3061 tctccttcac ggtcagggtc agttgccaga aatactttgt ccgacttctt cgcctctttc
     3121 cgcaaagccg caagaatatc gccttttccc cttattgtaa tgtattttgg ttcgtaatcg
     3181 tgttctacat cgattccaag tgaacttttc ggaagatccc gtacatgtcc ctgagaagcc
     3241 gcaactgtat aattgctgcc caaaaacttt ttgatcgttt tcactttcgc aggagactcc
     3301 acgataacnn nnnnnnnncg ataacaagat aatgtgcgat aaaaaattac tcctccatct
     3361 gagcggtatg tgcccatttt taacccaaag ctgtttttat gattttcaaa ctcaaatcaa
     3421 actgcaggaa aattcatttt aaacaataat ataatgattt tttgatattt cccgtatgta
     3481 cccgcgcaat tccaaagaaa ccaatatttc catcacctga cgtacttcca gttttgtttc
     3541 tttcaaaatt tcttccacgc tctttgggta caaaccgaga caactataca acaattcctc
     3601 ttgagtttca agcatttttt tatttttacc ttctttctct gtttttttac ctgataatcc
     3661 caattcttcc aaaaacattt ccggtgaaag taaaattccc gctccctgac ggatcaattc
     3721 attacatccg atactcatcc ggctatttac aggacccgga agcgcataca cttctctccc
     3781 ttgttctaat gccagatcga ctgtaataag cgagccgctc ttctctcccg cttccataac
     3841 aagcacaaca tctgacaatc cgcttataat tctgttccga gccggaaagt tttgaggaaa
     3901 cggaggcgtt ccaggcggca tctcggaaat aattccacct ccttgttcca atatatctac
     3961 ataaagcccg atatgctccc tcgggtaaca aacgtctacg ccacacccca ataccgcgaa
     4021 tgttttacca tttccaagaa gcgcccctct gtgacccatt ccatctacgc cgcgagccat
     4081 tccacttata atctgcaccc catgttctgc caaaactttt ccaaaatcaa ctgcatattt
     4141 ttcaccataa ggagtacatc tcctcgctcc cacgagagcc gcctttttcg cagctttatc
     4201 cggaaggctg ccttttacat aaagtgcata aggataatcc gaaatccgtt ttaatttttc
     4261 cggatattca tccgtaaaat gcggaataaa acgaattcct ttttcacaca gactttcata
     4321 ctctttttcc ggatcctgcc gttttgcctt caaaatttta tcttgctctg attcgtttaa
     4381 aaattctatt tttttcagct gtgtttattc tatataagaa atcgcttctg ctgttttcat
     4441 gctttgacgc aggcggattt tttttcgc
//
LOCUS       DBMDXF010000152         4465 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4678_length_4465_cov_2.406508, whole genome shotgun sequence.
ACCESSION   DBMDXF010000152 DBMDXF010000000
VERSION     DBMDXF010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_4465_cov_2.406508"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            253..2706
                     /locus_tag="ACZPD4_06590"
     CDS             253..2706
                     /locus_tag="ACZPD4_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378763.1"
                     /translation="MKNKFIRKWLVILTVAAMMIPAGIPANAETVEEGENIAPTAGIS
                     AETPNVTAETQKEEISAEMPDMTAETQNEEKSDVQAEGQNQAKQGVLEAVRQNTEKAE
                     EASDAVDVQAEERTAGEVQIGEQIYPTLTEAFAAAKDGDVLRLLENAEVSQPILLTKN
                     VTLDTNGFTVSAAAGFKGNFMFTNRGTFNIVGSGKIDGSVGTYPLIMINNTGGAVLNI
                     QSSEITGQGVVQNVGGIVNITGGTLTASARNTVLSQFGEVHIGASAHLMAAGEGKSAV
                     QLIGAKMTLSGDAVLSGNGGIDVFNSNRNEEGTVSAQVEITGGRIETKDFPVSGNNQE
                     SAGAEVAITGGSLKNISGGTAIYWPMEGQLTIGGNAVIEGGTGIEAKMGTITIKDNAV
                     ITGSGEWKEEKPQNGGANPEGSAFLGSAQMYDGCINDSSLVVNILGGTLKSVNGNAVT
                     IYNTEEKSDVRADISVTGGSLQPAAGKGAVKVITSGDNTTRFDPDKKTLDTMKSNTTV
                     KVAKDVAAAATDRDGKTTYYNTVEEALGSNTEDGNIHIYINENSSVKQEALEGENVIL
                     TVAPGVVLEVTSGIDGMIVKETVHEDGSKTYELVNAEELSAPKNVTVTADCKTVHIGK
                     KIRLKASAEHDTKQVNYLYRWYKDGALLNGAVSAELEVTESGNYAVEVFAVLEKDGTT
                     LTSLGAKSDPVKCTVTPHEYEEKWSSDGKVHWHECTICKNKTDVAEHTFGEWKVTEKA
                     TEKKDGRKERSCTVCGHKETAVIKAAGKTEEPRKESDKTASVKTGDKTDPAVYIFFVI
                     LTGGMIAILSAGNRKNRRK"
     gene            complement(2841..3725)
                     /gene="dapA"
                     /locus_tag="ACZPD4_06595"
     CDS             complement(2841..3725)
                     /gene="dapA"
                     /locus_tag="ACZPD4_06595"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1378764.1"
                     /translation="MSIFKGAGVAIVTPMKSDLEVNYDKLAELLEEQIAGKTDAIIIC
                     GTTGESATMSEEEHSKVIRFAIERVNHRIPVIAGTGSNSTRTAIQLSQEAAKDGADGL
                     LLVTPYYNKATQNGLIAHYTQICNSVDIPAILYNVPSRTGCAIQPTTVAHLVKNVDNI
                     VGIKEASGDISVGAKIMHLCDGNIDLYSGNDDQVVPLLSLGGIGVISVLSNVAPTYVH
                     DMVTKYLSGDTAGSCKMQLDALPICDALFCEVNPIPVKAAMNMMGKEVGSLRAPLTEL
                     EEPHKELLKKAMTEFGLL"
     regulatory      complement(3823..3996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1423 a          813 c         1141 g         1088 t
ORIGIN      
        1 tcatatcgga tttgtcaaga aaaatatagt tattttttga aaaaatttga aaagtttcga
       61 taaaaacgga acataactta aatggatttc aggagaggtc atgaaacagg cgttttgaga
      121 tagaatggtt aattgttgta aataaagtcg gaatttactg aaaatatatt gaagaagcag
      181 aaaaagcatc gtattattaa agaaaaaaca cttaatgcat gacgattatg tatggtggga
      241 aaggagagta agatgaaaaa caaatttatt aggaaatggc tggttatttt aactgtggcg
      301 gctatgatga tcccggctgg aattccggca aatgcagaaa cagtggaaga aggcgaaaat
      361 atcgcgccga cagcgggaat atcagcagaa acgccgaatg tgacagcaga aacacaaaag
      421 gaagaaatat cagcagaaat gccggatatg acggcagaaa cacagaatga agaaaaatcg
      481 gatgtgcagg cagaaggaca aaatcaggca aaacagggtg ttttggaagc ggtacggcag
      541 aatacagaaa aagccgaaga agcttcagat gcagtcgatg tacaggcgga ggaaaggacg
      601 gcaggggaag ttcagatcgg agagcagata tatccgactc ttaccgaagc atttgcagcg
      661 gcaaaagacg gggacgtact gcggctttta gaaaatgcag aagtttcaca accgatcttg
      721 ctgacaaaga atgtgacgtt agatacaaat ggatttactg tatcggcggc agcaggattc
      781 aaaggcaatt ttatgtttac gaatcgggga acattcaata ttgtcggaag cggaaagatt
      841 gacggcagtg tgggaacgta cccgctgatc atgatcaata ataccggcgg cgcggtttta
      901 aatatccaaa gcagtgagat caccggtcag ggagttgtgc agaatgtagg cggaatcgtg
      961 aacatcacag gcggtacgtt gacggcatca gcaagaaata cggttctttc acagttcggc
     1021 gaagtgcata taggcgcatc ggcccatctt atggcagcag gagaagggaa atctgcggtt
     1081 cagttgatcg gagcaaagat gactctttcg ggggatgcgg tgctgtcagg aaacggcggg
     1141 atcgatgtgt ttaacagcaa cagaaacgaa gaaggtactg tatcggcgca agtagagatt
     1201 acaggcggta ggatcgagac aaaagatttt ccggtttcgg gaaacaatca ggagtcggca
     1261 ggagcggaag ttgcgatcac aggcggaagc ctgaaaaata tatcgggcgg aacagctatt
     1321 tactggccga tggaaggaca acttacgatc ggcggcaatg cggtgatcga aggaggaacc
     1381 gggatcgaag caaaaatggg aacaattacg atcaaggata atgcggtcat cacaggaagc
     1441 ggcgaatgga aagaagaaaa gccgcaaaac ggtggggcaa atcctgaagg ttcagcgttt
     1501 cttggatcgg cgcagatgta cgacggctgc atcaatgatt cttctcttgt cgttaatatt
     1561 ctcggtggaa cgcttaaaag cgtaaacggt aatgcggtca cgatctataa tacagaagaa
     1621 aaatcagatg tgagagcaga tatttctgtg acaggcggat ctttgcagcc ggcagcagga
     1681 aaaggtgcgg tcaaagtaat tacaagcgga gataatacga cccgttttga tccggataaa
     1741 aagactcttg acacaatgaa atccaatact accgtaaaag ttgcaaagga tgtagctgct
     1801 gcggcaacgg acagagacgg aaagacaaca tattataata cagtagaaga agcgcttgga
     1861 tcgaatacgg aagacggaaa tattcacata tatattaacg aaaactcttc tgtaaaacag
     1921 gaagctttgg aaggagaaaa cgtaatattg acagttgctc ccggagtcgt tttggaagtg
     1981 acatccggaa ttgacggaat gatcgtaaaa gaaactgttc atgaagacgg atctaaaaca
     2041 tatgaacttg ttaatgcaga agaactttca gcaccgaaaa atgtaaccgt tacggcagac
     2101 tgcaagactg tacacatcgg caaaaagatc cggctgaaag cttcggcaga acacgataca
     2161 aagcaggtga attatcttta tcggtggtac aaagacggag cgttgttaaa tggagctgtt
     2221 tctgcggaat tggaagttac agaaagcgga aactacgctg ttgaagtatt tgcggttctg
     2281 gaaaaagacg gaacaacgtt aacttcttta ggcgcgaaga gcgatcctgt taaatgtacg
     2341 gtgacgccgc atgaatatga agaaaaatgg agttcggacg gaaaggttca ctggcacgaa
     2401 tgtacgatct gtaaaaataa aactgatgtg gcagagcata cattcggaga atggaaagtg
     2461 acagagaaag cgacagagaa aaaggacgga agaaaagagc ggagctgtac tgtatgtggt
     2521 cataaagaaa cggcggtgat aaaagcagca ggcaaaacag aagagccgcg gaaagaatca
     2581 gacaaaacgg cttctgtgaa aaccggtgac aaaacagatc cggcagttta tattttcttt
     2641 gtcattttga caggcgggat gatcgcaatc ttatcggcag ggaacagaaa gaacagaaga
     2701 aaatagaata gatgaaaata gaataaaaga aaatcgaaac aatgaaaaag caagaggtcc
     2761 gttccactaa gtggagcaga cctctttaat gattaattat agcagaatat atgcagtaaa
     2821 aagattcaaa acagaaaatt ttacagcaga ccgaattcag tcattgcttt tttcagcagt
     2881 tctttgtgag gctcttctaa ttctgtaaga ggagctctaa gagaaccgac ttctttgccc
     2941 atcatgttca tagcggcttt tacagggatc ggattgactt cgcagaaaag agcgtcgcag
     3001 atcggaagcg cgtctaactg cattttgcag cttcctgccg tatcgcctga aagatatttt
     3061 gttaccatgt cgtgaacgta agtcggagct acgttggaaa gtacggagat aacaccgatt
     3121 ccgccaaggg aaagaagagg tactacctga tcatcgttcc ctgagtaaag gtcgatgttt
     3181 ccgtcgcaca gatgcatgat ctttgcaccg accgaaatat cgccggaagc ttctttgatg
     3241 ccgacgatgt tatctacatt ttttacaaga tgagctacag ttgtaggctg gatcgcacat
     3301 cctgtacggc tcggcacatt ataaaggatc gccgggatat cgacggagtt acaaatctgt
     3361 gtatagtgtg cgatcaaacc gttttgcgtc gctttgttgt agtacggtgt gacaagaaga
     3421 agtccgtctg caccgtcttt ggctgcttcc tgggaaagct ggatcgctgt gcgtgtagag
     3481 ttggatcctg tgcctgcgat caccgggata cggtggttta cacgttcaat ggcaaaacgg
     3541 atcactttgc tgtgttcctc ttcggacatt gttgcagatt ctccggtcgt tccgcagatg
     3601 atgatcgcat ctgtttttcc tgcaatctgt tcttcgagta gttccgcaag tttatcgtaa
     3661 ttgacttcaa gatctgattt cataggagta acgatcgcta cgcctgcgcc tttgaaaatt
     3721 gacataatta aatcctcctt aaatgtttgc gttcgtttga tagtgctttg cgtaaataaa
     3781 agaaacggca ggatgcgccg tctcaaaata tactttacgg tatacttcga tagctctcca
     3841 tcttgcgatg acagtcatgg cgctgttgac gacatgccca aggaaaaggg ttcaagtctc
     3901 catatccttt cggcatcttc ccctttctga tgagttcttc tgctcttgaa tacatccgtc
     3961 atcgtactta tgaaaaatgc gacctctatc tcgttccttt attagttatt atatgaaaaa
     4021 aattgttttg cgaacctgaa atgagttata caaaacgctt catctaatat aaaaaaatca
     4081 taacacccac gtggaatgat gtcaactgtt ttgctctttg acaagcaaat tatttcatgg
     4141 taaaatatat gcatctgcag tgtgaatgac aagattttca tttgcacttt ttttatgctg
     4201 tagcaaattt taatttctta cagcataaaa cccttcggga ggatgtgtat gccgcatcga
     4261 ggaagtctgc cgctctgcgg ctctgcggcg cgcaaaggcc aagcccttta tgagtaaggg
     4321 aaggagggcg ttgaggtatg ttagctcact ttgttatgta tgtataggta attgcgaaac
     4381 aagtccgcaa tcctgactga aaacaggcga aggctcctgt catgcaggat tcttgggata
     4441 ggaagtgaaa atcgttcatt tcttg
//
LOCUS       DBMDXF010000153         4451 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4698_length_4451_cov_1.743908, whole genome shotgun sequence.
ACCESSION   DBMDXF010000153 DBMDXF010000000
VERSION     DBMDXF010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_4451_cov_1.743908"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="ACZPD4_06600"
     CDS             <1..496
                     /locus_tag="ACZPD4_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR1378765.1"
                     /translation="VTTFMDEHLAGTKRRDEDKILTKTMINNYSKNNLLPPSVKKKYS
                     KEHVLLLIFIYYFKSILSIKDIETVLAPLRDKYFSDEAALSLSELYTEVCKLEKSRIK
                     PLSESVQEAYESSFSAFSNIEDDADQKELRLFAFICSLSFDVYLKKVMIEKIIDELSA
                     QKED"
     gene            complement(554..844)
                     /locus_tag="ACZPD4_06605"
     CDS             complement(554..844)
                     /locus_tag="ACZPD4_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR1378766.1"
                     /translation="MSKKIRTEAVDYLFEAILSLKNKGECYTFFEDICTINELLSLSQ
                     RFEVAKMLREQKTYLEIAEKTGASTATISRVNRSLNYGNDGYDMVFERIDKK"
     gene            complement(1031..1759)
                     /locus_tag="ACZPD4_06610"
     CDS             complement(1031..1759)
                     /locus_tag="ACZPD4_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1378767.1"
                     /translation="MRFEADTHSHTLASGHAYSTIKEMAAAAEAKGLKALALTEHAPK
                     MPGTCGLFYFQNLDVVPRKCGGIRLLMGAEVNIMDETGRIDLPESTCRDLDIVIASIH
                     PPCYGMGHTEEENMRAYEAVMKKPYINIIGHPDDGRFQIDYERLVKMAKETGTLLEVN
                     NSSMRPSSSRKGTRENILTMLDLCKQYDVPVTTGSDAHVDVDAGNFTYVKEVLKFCDF
                     PEELVVTTKFDVLKQYLNMNTFGM"
     gene            1999..3150
                     /locus_tag="ACZPD4_06615"
     CDS             1999..3150
                     /locus_tag="ACZPD4_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378768.1"
                     /translation="MKCPICGNKLEIKNKQIGTDNSGDPVFNEYAVCRDCKKQWNLDK
                     QRAKKAAAKASAKKAEAADKTSRKKNESDVAQKTDENKIKKAHKEPEAAHTGSPKKRA
                     AAEASPKASDRVKSPAQNQKRRPDEKATDEKTVRIQENPEKKVPKRKPSSKQTTEKSA
                     PNEDKRYSNIPPEQVRTKREKAVKKGYDDMLATGVLDTDKVKKASSKSSKNKNDKYDT
                     KASRKMPEPEIDDYEDDYYESVPRFRAVRIILGIISLAGFGFFTYKGFMTGLNGTTDG
                     GGTSGTLYIILALCMLVSSLLYFIMQKSNTIFAFLLPMLFYLGSGVFAFLKRGDDLQL
                     LIAAIVSGVLAIISLILAITSRGGSDYDDSEENYDEAFEDDYAEEEFDD"
     gene            3314..3814
                     /locus_tag="ACZPD4_06620"
     CDS             3314..3814
                     /locus_tag="ACZPD4_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1378769.1"
                     /translation="MKKQLLTSLFAFLLVFSLCMGCSKAETGDKTTASDSFEVSSQKT
                     SPVKITLNEVAHSVFYAPLYVAIEKGYFKDENIDLTLVTGFGADKTMTAVISGEADIG
                     FMGSEASVYMYSEGASDYVVNFAQLTQRAGNFLVARQDNEHFQWADLKGKTVLGGRKG
                     GVHILM"
     gene            complement(3921..>4451)
                     /locus_tag="ACZPD4_06625"
     CDS             complement(3921..>4451)
                     /locus_tag="ACZPD4_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HIR1378770.1"
                     /translation="RTHKKERDEYAKIKKVLAQKFPYDIDGYCDGKEEFVRKIEKLAL
                     SQFDGTWDKMYLAARNVQKKREISPLVEAGGVSAAILTEKGNIYVGVCIDTACSLGMC
                     AERNAIANMITNGENGICRLIAVDRNGKAIPPCGACREFMVQLMPDHYHSIQIMMDYE
                     NKKMVTLGDITPNWWI"
BASE COUNT         1374 a          974 c          786 g         1317 t
ORIGIN      
        1 ggtaacaact tttatggacg aacatcttgc cggaacaaaa cgccgtgacg aagataagat
       61 tttgacaaaa acaatgataa acaactactc aaaaaataat cttcttcctc cgtccgtcaa
      121 gaaaaaatat tctaaagaac atgtacttct tcttatattt atttactact ttaaaagcat
      181 tctttctatc aaagatattg aaaccgtact tgctcctctg cgggacaaat atttttcaga
      241 cgaggcggcg cttagcctct ccgaactgta taccgaagtt tgcaagcttg aaaaatcccg
      301 tatcaagccg ctttccgaat ccgttcaaga agcatacgaa agttcattct ctgctttttc
      361 aaatatagaa gatgacgccg atcaaaaaga acttcgtctt tttgcattta tttgctcttt
      421 aagctttgac gtctatttga aaaaagttat gatagagaaa atcatagacg aactttctgc
      481 tcaaaaagaa gattgacgag tacaaaagta cgtattaaaa atgccggaca cgcatgaacg
      541 ctccggcatt tttttatttt ttatcaattc tttcaaaaac catgtcatat ccatcattgc
      601 cgtaattaag agaacggttc acccggctga tcgttgccgt tgaagcgcct gtcttttccg
      661 caatttcaag ataagttttc tgctctctga gcatttttgc cacttcaaat ctctgtgaca
      721 gagacagcag ctcgttaatc gtacaaatat cctcaaaaaa agtataacat tctcctttat
      781 ttttcagact gagaatcgct tcaaaaagat agtcaactgc ctctgtcctg atttttttac
      841 tcatataaac ttctcctttt caacaccgtg gcatatatcc gagcaacctc tgcatccaca
      901 gcaattattt caagcactgt tatattttag cacattaaat tcttgaagtc catacgttaa
      961 tcagttaaat ttataaagtt ttacaaaagt accttatccc tttcatctgc atacaaaagt
     1021 ccgctctctc ctacataccg aaagtgttca tattcaaata ttgttttaat acatcaaatt
     1081 ttgtcgtaac aacaagttcc tccggaaaat cgcaaaattt aagcacttct tttacataag
     1141 taaaattgcc tgcatctaca tctacatgtg catcactgcc tgtcgtaacc ggaacatcat
     1201 actgtttaca aagatcaagc attgtaagaa tattttcacg agttcccttt ctgctgcttg
     1261 acggtctcat ggaagaatta tttacttcca aaagcgttcc cgtctctttt gccatcttca
     1321 caagacgttc gtaatcaatc tgaaaacgac cgtcatcagg atgcccgata atgttaatat
     1381 acggtttctt catcacagcc tcgtatgctc tcatattttc ctcttctgtg tgccccatcc
     1441 catagcatgg cggatgaatg ctcgcaataa ctatatccag atcacggcag gtactttccg
     1501 gaaggtcgat cctgcctgtt tcatccatta tatttacttc cgctcccatt aagaggcgaa
     1561 ttcctccgca ttttcttgga acgacatcca ggttttgaaa ataaaacaac ccgcacgttc
     1621 ccggcatttt cggagcatgt tccgtaagcg caagcgcttt taagcctttt gcttctgctg
     1681 ccgccgccat ttcttttatc gtgctgtatg catgtccgct tgccagcgtg tgtgaatgtg
     1741 tatctgcttc aaatctcata atttctcctt tcgcctttgt tacaaattct gctgtcattt
     1801 tttgcaatat ctgtataatt atgacgctgt tttttatctt ttaattttga tatcatatca
     1861 catatcgcgc tatattttcg atcatatttg atatttttcg atttattgtt acatttttca
     1921 ttgttctaac tcatttgaaa tgttataata aaaccattat aaattcgatt taacgaagag
     1981 agaggagtat taatttttat gaaatgtccg atatgcggaa acaaacttga gatcaaaaac
     2041 aaacaaatcg gcactgacaa cagcggagat cctgtcttta atgaatatgc agtctgccgt
     2101 gactgtaaaa aacagtggaa tttagacaag caacgtgcaa aaaaagcagc ggcaaaggca
     2161 tctgccaaaa aagccgaagc ggcagataag accagccgca aaaaaaacga gagcgacgtt
     2221 gctcaaaaaa cagacgaaaa caaaatcaaa aaggcgcaca aagagccgga agcagctcat
     2281 accggcagcc ccaaaaaacg cgctgctgca gaagcttccc caaaagcttc cgaccgggtg
     2341 aagtcacctg cgcaaaatca aaaacggcgg cctgatgaaa aagcaactga cgagaaaaca
     2401 gttcggattc aggaaaatcc cgaaaagaaa gtcccgaaaa gaaagccttc atccaaacaa
     2461 actactgaaa aatcagctcc gaacgaagat aaacggtaca gtaacatccc accggaacaa
     2521 gtccgcacaa aacgggagaa agctgttaaa aaaggctatg atgatatgct tgcaacagga
     2581 gttcttgata ctgacaaagt aaaaaaagcc tcctcaaaat catccaaaaa taaaaacgac
     2641 aaatatgaca caaaagcttc gcgtaaaatg cctgaaccgg aaatcgacga ttatgaagat
     2701 gattattacg aaagcgttcc gagattccgc gcagtgcgca tcattctcgg gatcatctct
     2761 ctcgcaggat tcggattctt tacttataaa ggattcatga ccggacttaa cggcacaaca
     2821 gacggcggcg gcacaagcgg cactttatac attatccttg ccctttgtat gctcgtatct
     2881 tcacttctct attttatcat gcaaaagtca aatacgattt ttgctttcct tcttccgatg
     2941 cttttttatc tcggaagcgg cgtatttgca tttttgaaac gtggagatga tctgcagctt
     3001 ctcatcgcag ctattgtaag cggagtattg gcaattattt cactcatact tgcgatcact
     3061 tcccgcggag gatctgatta tgatgatagt gaagaaaatt atgatgaagc ctttgaagac
     3121 gattatgccg aggaagaatt tgacgattag aaagccataa ctgttttaga aaaactgaaa
     3181 tttaaaagca gagcgttact tacaggaatc cccaaaaccg ggagacttgc gtatcgctct
     3241 gtttttatat ccttttcact gtacggttat attttacata tattacaata aaaccaaatc
     3301 atctggagat ttcatgaaaa aacaactttt aacctctctt tttgccttcc ttcttgtatt
     3361 ttctttgtgt atgggatgct ctaaagccga aaccggagat aaaacgacag cttccgactc
     3421 ctttgaagta tcgtctcaaa aaacttcccc tgtaaaaatc acactaaacg aagtggctca
     3481 ttctgttttc tatgctccac tatatgtggc gattgagaaa ggatacttca aagatgaaaa
     3541 catcgacctc acacttgtca ccggatttgg agccgataaa acaatgactg ccgttatttc
     3601 cggagaagcc gatattggat ttatgggatc tgaagcttcc gtttatatgt acagcgaagg
     3661 agcttccgac tatgtcgtta attttgcaca gctcacacag cgagccggaa actttctcgt
     3721 tgcacggcaa gacaacgagc attttcagtg ggcagatctc aaaggaaaaa ctgttctggg
     3781 cggtagaaaa ggcggcgtac atatattaat gtaaatttag agagtattgt gattttatgt
     3841 aactgggtgg acagaatgaa tagtgcgaag agtatataaa agaagtggcg gaaataccaa
     3901 aaatgatatt tccgccatac ttatatccac cagttaggag tgatatctcc taatgtaacc
     3961 atttttttat tttcataatc catcatgatc tgaatagagt gataatggtc cggcattaac
     4021 tgtaccataa attcccggca ggcaccacaa ggcgggattg cttttccatt ccgatcaacc
     4081 gctatcaatc gacaaatgcc attctcgcca tttgtaatca tattggcaat cgcatttctt
     4141 tctgcacaca ttcccaaaga gcaagcagta tcgatacaaa caccaacata gatattccct
     4201 ttttcagtaa gaattgcagc ggagacgccg ccagcctcta caagcggaga aatctctctt
     4261 tttttctgta catttcgtgc cgcaaggtac attttatccc atgttccatc aaattgggag
     4321 agagcgagtt tctcaatttt acgcacaaat tcttcttttc cgtcacaata tccatcaata
     4381 tcataaggga atttctgtgc aagaactttt ttgatttttg catattcgtc tcgttctttt
     4441 ttatgagtac g
//
LOCUS       DBMDXF010000154         4440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4709_length_4440_cov_1.849735, whole genome shotgun sequence.
ACCESSION   DBMDXF010000154 DBMDXF010000000
VERSION     DBMDXF010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4709_length_4440_cov_1.849735"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="ACZPD4_06630"
     CDS             <1..285
                     /locus_tag="ACZPD4_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378771.1"
                     /translation="TAKDFSFNPSLNTKLTADTKKVTVTYGGQSADVAVSVKADPSED
                     KKPNTEKPDKGGAVQTGDNFNVTLLIGLVVLAGAVAGGAALTIFKRNKRK"
     gene            657..1142
                     /gene="greA"
                     /locus_tag="ACZPD4_06635"
     CDS             657..1142
                     /gene="greA"
                     /locus_tag="ACZPD4_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1378772.1"
                     /translation="MAEAKKRLLTYAGLKALEDELETLKVVKRKEVAAKIKEAREQGD
                     LSENAEYDAAKDEQRDIEARIEELEGILKNAEVVVEDEVDFDKINVGCTVKVFDITFD
                     EEMEFKIVGSTEANSLVGKISNESPVGQALIGKKVGDTVSVETQAGEIKYKVLEISRS
                     M"
     gene            1303..3231
                     /gene="lysS"
                     /locus_tag="ACZPD4_06640"
     CDS             1303..3231
                     /gene="lysS"
                     /locus_tag="ACZPD4_06640"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1378773.1"
                     /translation="MAQEQKNVQEQDLNQLRKVRREKLAELQGSGKDPFVITKYDQTH
                     HSVEVKENYEELEGKQVSIAGRMMSKRVMGKASFCNVQDLKGNIQSYVARDSIGEENY
                     KEFKKLDVGDVIGIEGEVFKTKTGEISIHASHVTLLSKSLQILPEKFHGLTNTDMRYR
                     QRYVDLIMNPEAKDTFIKRSKIISAIRRYLDGEGFMEVETPMLVANAGGAAARPFETH
                     FNALNEDLKLRISLELYLKRLIVGGLERVYEIGRVFRNEGLDTRHNPEFTLMELYQAY
                     TDYNGMMDLTENLYRHVAQEVLGTTKIVYNGVEMDLGKPFERITMVDAVKKYAGVDWN
                     EVKTLEEARKLADEHHVEYEEHHKKGDILSLFFEEFAEEHLIQPTFVMDHPIEISPLT
                     KKKPENPEYTERFEFFMNGWEMANAYSELNDPIDQRERFKAQEELLAQGDEEANTTDE
                     DFLNALEIGMPPTGGIGFGIDRMCMLLTNSAAIRDVLLFPTMKTQGGAKNEANNSVQA
                     KTEEKPAEKIDFSKVEIEPLFKDDVDFETFSKSDFRAVKVKECTAVPKSKKLLQFTLD
                     DGTGEDRTILSGIHEYYEPEELVGKTCIAITNLPPRKMMGIDSCGMLISAVHNEEGEE
                     KLHLLMVDEHIPAGAKLY"
     gene            3454..3830
                     /locus_tag="ACZPD4_06645"
                     /pseudo
     CDS             3454..3830
                     /locus_tag="ACZPD4_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421721.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            4060..>4440
                     /locus_tag="ACZPD4_06650"
     CDS             4060..>4440
                     /locus_tag="ACZPD4_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012493.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme subunit R
                     domain-containing protein"
                     /protein_id="HIR1378774.1"
                     /translation="MMYRLFLENYADDSELDNYTGEDGQIIYRSISANEMEVKKTSPQ
                     KGRYRAALILHDSFDKSTRSKWIDLFKDGKIDLLIVFQMLQTGFDAPRLKKLYLHRMV
                     KEHNLLQTLTRVNRPYKAMKFGHVV"
BASE COUNT         1538 a          721 c         1078 g         1103 t
ORIGIN      
        1 acggcaaaag atttctcctt taatccgagt cttaatacaa aactgacagc ggatacgaag
       61 aaagtaacag taacatacgg cggacagtcg gcagacgtag ctgtatcggt gaaagcagat
      121 ccgtcagagg ataagaaacc gaatacagag aaaccggaca aaggcggagc ggttcagaca
      181 ggagataact ttaacgtaac gctgctgatc ggacttgtag ttcttgcagg tgcggttgca
      241 ggcggagcgg ctctgacaat cttcaaaaga aacaaaagaa aataagcctt atccgtaaat
      301 agtacggaaa aatgcagggc tgccacttag tggcggccct gtttgcattg gagcggcaat
      361 gaacgaaaga cagaaggatc aagaccttgg aaagccgcct gtaatccaag aatgtgtctt
      421 tgggaactta cggtaattcc ggcataacgc tgtattgtta ataagcgcta tattttctgc
      481 gcaatatcat gtaaaagctg tcttgtttac gccaaaaacc ttgacattaa tggggttgat
      541 ggttataata ttgaaattgt gaaataaact aaaagagaca gcgtgcaggg acaagttttc
      601 ctgtcgggaa tagacaaaaa acagtgaaaa agaatgacag aaaaggagcg ttatcaatgg
      661 cggaggcaaa gaaaagatta cttacttatg ccggattaaa ggcacttgaa gatgaacttg
      721 aaactcttaa agttgtcaaa cggaaagaag ttgcagctaa gatcaaagag gcaagagagc
      781 agggagatct ttccgagaat gcagagtatg atgcggcaaa agatgaacaa agagatatcg
      841 aagcgcgtat cgaagaactt gaaggcattc tgaaaaacgc agaagtggtt gttgaagatg
      901 aagttgactt tgataagatc aatgtcggat gtacggtaaa ggtatttgac ataacattcg
      961 atgaagagat ggaatttaaa attgtgggat caacagaggc aaacagcttg gtaggaaaga
     1021 tttcaaacga gtctccggtt ggtcaggcat tgatcggaaa aaaagtggga gatacggtaa
     1081 gcgtagaaac acaggctgga gaaatcaagt acaaagtgct tgagatcagc cgttcaatgt
     1141 aaacagatga ttataaacag acctcttttt aagaggtctg aattcgcatg tatgggaatg
     1201 ttttaaagag gcgaaaaagg cagataaaga gaaaaaagcg taacggaata ataagagata
     1261 aagcaaaaca gagaaaagtt atccgggaaa ggagataaga aagtggcaca ggagcagaag
     1321 aatgtacagg aacaggatct gaatcagttg agaaaggtac gccgtgagaa acttgcagag
     1381 ctgcaaggaa gcggaaaaga tccgttcgtg attacaaaat atgatcagac acatcacagt
     1441 gtagaagtaa aagaaaatta cgaggagctt gaaggaaagc aggtttccat cgcaggacgt
     1501 atgatgtcaa agcgagtaat gggaaaagca tctttttgta atgttcagga tttaaaagga
     1561 aatattcagt cttatgtagc aagagacagc atcggtgagg aaaattataa agaatttaag
     1621 aaacttgatg tcggtgatgt gatcggaatc gaaggagagg tattcaagac aaaaacagga
     1681 gagatttcca ttcatgcatc tcatgtaaca cttctttcca agagtcttca gattcttccg
     1741 gagaagttcc atggactgac gaatacagat atgcgttatc gtcagagata tgtggatctt
     1801 atcatgaatc cggaagcgaa agatacattt atcaaacgtt ctaagatcat cagcgcgatc
     1861 cgcagatatt tggacggcga aggatttatg gaagtggaga ctccgatgct tgttgcaaat
     1921 gccgggggag ctgcggcaag accgtttgag acacatttca atgctttaaa tgaagacttg
     1981 aaactgagaa tttctcttga actttatctg aaacgtctga tcgtcggagg tcttgaaaga
     2041 gtgtacgaga tcggtcgtgt attcagaaac gaaggacttg atacgagaca taatccggaa
     2101 tttacattga tggagcttta tcaggcatat acagattata acggaatgat ggatcttaca
     2161 gaaaatcttt accgccatgt ggcacaggaa gttctcggca caacgaagat cgtttacaat
     2221 ggcgttgaga tggatctcgg aaaaccgttt gaacgtatta caatggtaga tgccgtgaag
     2281 aaatatgcag gcgttgactg gaatgaagtt aagactttgg aagaggctcg caagcttgca
     2341 gacgagcatc atgtagaata tgaagagcat cataagaaag gtgatatctt gtcattgttc
     2401 tttgaagagt ttgcagaaga acatttgatt cagccgacgt tcgtaatgga tcatccgatc
     2461 gagatttcac cgcttacgaa aaagaaaccg gagaatccgg aatatacaga gcgtttcgag
     2521 ttctttatga acggctggga aatggcgaat gcatattcag agctgaatga cccgatcgat
     2581 cagagagaac gtttcaaagc tcaggaagaa cttttggcac aaggtgacga agaggcaaat
     2641 acaacagatg aagatttcct gaatgcactt gagatcggta tgccgccgac aggaggtatc
     2701 ggattcggta tcgacagaat gtgtatgctt ttaacaaaca gcgccgcgat cagagacgta
     2761 ctgcttttcc caacaatgaa aacacagggc ggagctaaga atgaagcaaa caattcagta
     2821 caggcaaaaa cagaggaaaa accggcagaa aaaattgatt tttccaaagt tgagatcgag
     2881 ccgttattta aagacgatgt agattttgag acattttcta aatcagattt cagagcggta
     2941 aaagtaaaag agtgtacggc agtgccgaaa tctaagaaac ttctgcagtt cacattggat
     3001 gacggaacag gagaggatcg tacgatcctg agcggaatcc atgaatatta tgagccggaa
     3061 gaattggttg gcaagacatg tatcgctatt acaaatctgc cgccgagaaa gatgatggga
     3121 attgactcct gtggtatgtt gatttcagcg gttcataatg aagaagggga agaaaaactt
     3181 catcttctga tggttgatga gcatattccg gcaggcgcaa aattgtacta attaatgtga
     3241 aataaaataa aaaatgattt ttatatcaat tttgtaccat aatgaaaaca gatttaaaac
     3301 gttataaaga gatataaaga gataggctca tggaatgtat tttctatgag cctgtttgtg
     3361 ttatgtaaag gcagatgaca gtagatgaaa gattatcgaa ccatgattat tattatgagt
     3421 ggaagaaaaa caggattata ttttgaaaat tacgaaaaag agtgggtact tgacggagca
     3481 actgttctct acggaagcgc atcggaacta agagcaactc ttgaatatga ttttttgcag
     3541 gaaaaaactt tatttacaaa gggttttcga tggaggaaat cattcatcat cttgcagtct
     3601 ttgtttcaag attatggcaa atccatatct ttggtgagga aaatacaaga gcgacggcag
     3661 tatttttaat caagtatttg agaacgctcg gattttctgc cacaaatgat atttttgcga
     3721 aaaatgcttg gtattttaga aatgcacttg tcccggcaaa ttatacgaac ctgcaaaagg
     3781 gtatccacga aacaaccgag tatttggagg tattcttgag gaacttgctg aggttataca
     3841 taagaaggac tggctcaaat agcaaatgta tcagttcaga caattacaca agtgcagtaa
     3901 aaaaagcaaa ctgttaaaga aattgatgtt tatgcccatg agaactatgt acatccttta
     3961 ctcgactata taatcaatga tttgcaggat ttccgaagca agtattctga cccatcattg
     4021 ggagcacttg ccgtatgcaa cagtaaagaa caggcgataa tgatgtatcg gctgtttttg
     4081 gaaaactatg cagatgattc tgaacttgac aattatacag gtgaagacgg acaaataatt
     4141 taccgtagta tttcggcaaa tgaaatggag gtcaagaaaa cttcaccgca aaaaggacgt
     4201 tatcgcgctg cgcttatctt gcatgacagt tttgacaaat ctacccgttc caaatggatt
     4261 gatttattca aagacgggaa aattgacttg ctcattgttt tccaaatgct acaaaccggc
     4321 tttgatgctc cacgattgaa gaaattgtac ctgcaccgta tggtcaagga acataacctg
     4381 ctgcaaacgc ttacacgtgt caaccgtccg tacaaggcta tgaaattcgg gcatgtagta
//
LOCUS       DBMDXF010000155         4438 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4711_length_4438_cov_2.041042, whole genome shotgun sequence.
ACCESSION   DBMDXF010000155 DBMDXF010000000
VERSION     DBMDXF010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_4438_cov_2.041042"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            107..1792
                     /gene="ilvB"
                     /locus_tag="ACZPD4_06655"
     CDS             107..1792
                     /gene="ilvB"
                     /locus_tag="ACZPD4_06655"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR1378775.1"
                     /translation="MQLNGAEIVIECLKEQGVDTVFGYPGGAILNVYDELYKHRDEIR
                     HILTSHEQGASHAADGYARATGKVGVCLATSGPGATNLVTGIATAYMDSIPIVAITCN
                     VGVSLLGKDSFQEIDIAGITMPITKYSFIVKDVTKLADTIRKAFRIAKTGRPGPVLVD
                     IPKDITAKVTEYIPEELGKCTKEDSSRKTPRMDEQRFDRVISMLEEAEKPFVFVGGGA
                     VLSGASQELKEFVDKLDAPVTDSLMGKGAFPGTDPRYTGMLGMHGTKASNFGVSECDL
                     LVVMGARFSDRVTGNTEKFAKNAKIIQLDIDPVEINKNVLITEEIIGDLKDVLKTLNK
                     RLKQQNHAEWLEKIQGYKEKYPLKYHPNVLSGPFLVEEIYRQTNGEALIVTEVGQHQM
                     WAAQYYKYTKPRTLLTSGGLGTMGYGLGAALGAKTGCPDKIVVNIAGDGCFRMNMNEI
                     ATAVRHNIPIIQVVVNNHVLGMVRQWQDLFYDERYSATVLRDAVDFVKLAEAMGAEGM
                     RAVSQEEFKEAFSKALTLNRPVVIDCQIDSDDKVWPMVAPGAAISEAFDEDDM"
     gene            1861..2943
                     /gene="serC"
                     /locus_tag="ACZPD4_06660"
     CDS             1861..2943
                     /gene="serC"
                     /locus_tag="ACZPD4_06660"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR1378776.1"
                     /translation="MSRVYNFSAGPAVLPEEVLKEAAAEMLDYNGTGMSVMEMSHRSK
                     AFEEIINSAEQDLRDLMNIPDNYKVLFMQGGASQQFAMIPMNFMKNRVADYIITGQWA
                     KKAAKEAEKYGKVNRIASSEDQTFSYIPDCSDLPVSEDADYVYICENNTIYGTKFKEL
                     PNTKGKTLIADVSSCFLSEPVDVEKYGMIYGGVQKNIGPAGVVIAIIREDLITEDVLE
                     GTPTMLTYKTHADAKSLYNTPPAYGIYICGKVFKWLKAQGGLAAIKEKNEKKAKVLYD
                     FLDQSKMFKGTVVKKDRSLMNVPFVTGDADLDAKFVKEAKEAGLENLKGHRSVGGMRA
                     SIYNAMPYEGVVALVEFMKKFEEENA"
     gene            2946..4109
                     /locus_tag="ACZPD4_06665"
     CDS             2946..4109
                     /locus_tag="ACZPD4_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR1378777.1"
                     /translation="MYKYHCLNPISAVGLDQLDENYVNVETAKGADAILVRSAKMHEM
                     EFDKNLKVIARAGAGVNNIPLERCAEEGVVVFNTPGANANGVKELVIAGMLLAARDII
                     GGINWVQEYEEDGDIAKITEKKKKVFAGTELEGKKLGVIGLGAIGVLVANAAVHLGME
                     VYGYDPYVSVDSAWRLSRNIHHAKTADEIYKECDYITVHVPALEDTKGMINKDAISLM
                     KKGVVILNFARDVLVNQEDIVDALVSEKVRCYVTDFPTKEIVGVRGAIVIPHLGASTE
                     ESEDNCAKMAAAEVKDFLENGNITHSVNFPDCDMGAKGEGERITILHKNIPNMIGQFT
                     ALLAEKNMNIEVMTNKSRKEYAYTMLDVDGTVSEDVEAQLAAVEGVLKVRVIR"
BASE COUNT         1449 a          688 c         1172 g         1129 t
ORIGIN      
        1 cacatccgga aacagaggtg cgattctgga aataccgaaa gaaaaataag agcgcggtta
       61 caggctgtgc ggataagaga tacaggatgt ttaaaggagt tatggcatgc agttaaatgg
      121 agcagaaatt gtaatagaat gtttgaaaga acaaggagta gacacggttt tcggctatcc
      181 gggcggtgcg attctcaatg tatatgatga attgtacaaa catcgggatg aaatcaggca
      241 catccttacc tcccatgaac aaggggcttc tcatgcggct gacggatatg cgagagcaac
      301 gggaaaagtc ggcgtctgcc ttgctacgag cggaccgggg gcgacgaatc ttgttaccgg
      361 aattgcaacg gcatatatgg attcaattcc tattgtggca atcacctgca atgtaggggt
      421 atcacttctt ggaaaagaca gttttcagga aatagatata gcgggaatta caatgccgat
      481 tacgaaatat agttttattg taaaagatgt gacaaaattg gcggatacta tccgtaaggc
      541 atttcggatt gcaaagacag gacgtcccgg gccggtgctt gtcgatatac cgaaagatat
      601 tacggctaaa gtaacggaat atattccgga agaattggga aaatgcacaa aggaagattc
      661 ctcgagaaaa actccgagga tggatgaaca acggtttgac cgggtgattt ctatgttgga
      721 agaagcagaa aaaccgtttg ttttcgtcgg aggcggagcg gttctttcag gggcgagtca
      781 agaattaaaa gagtttgttg acaagctgga tgctccggtt acggattctc ttatgggcaa
      841 aggcgctttt ccgggaacag atccgcggta tacaggtatg cttggcatgc atggaacgaa
      901 agcgtccaat ttcggagtaa gtgaatgtga cctcttggtt gttatggggg caaggttcag
      961 tgatcgtgtg acggggaata cagaaaaatt tgcaaaaaat gcaaaaatta tacagttaga
     1021 tatcgatccg gttgaaatta ataaaaacgt tttgattacg gaagaaatca tcggcgatct
     1081 aaaagatgtt ttaaagacgc ttaataagcg tttaaaacag cagaatcatg cagaatggct
     1141 tgaaaaaata caagggtata aagaaaaata tcctctgaaa tatcatccga atgtattatc
     1201 gggaccgttt cttgtagaag agatctatcg tcagacaaac ggtgaagcgc tcattgttac
     1261 ggaagtggga cagcatcaga tgtgggctgc acagtattat aaatatacaa aaccgagaac
     1321 ccttcttacg tcaggcggac ttggaacgat gggatatggt cttggagcgg cgcttggcgc
     1381 aaagacagga tgtccggata aaatcgtagt aaatattgcg ggagacggat gtttccgaat
     1441 gaatatgaat gagattgcga ctgcagtccg gcacaatatt ccgattatac aagttgtagt
     1501 gaataatcat gtgcttggga tggttcgtca atggcaggat ctcttttatg atgagaggta
     1561 ttcggctacc gtgctcagag atgcagttga ttttgtgaaa cttgcagaag ctatgggagc
     1621 agaaggaatg cgggctgtgt cgcaggaaga atttaaggag gcgttctcta aagcgctgac
     1681 attgaatcgt ccggttgtga ttgactgcca gatagacagt gatgataaag tgtggcctat
     1741 ggtagcgccc ggggccgcaa tcagtgaagc gtttgacgaa gatgacatgt aaaagtctgc
     1801 atgggcatat gtgtaattat aaaaacaaat aaattaaaaa taaaaacagg aggaaggaaa
     1861 atgagcagag tatataactt ttcagcagga ccggccgtgc tgccggaaga ggtattgaaa
     1921 gaggctgccg cagaaatgct tgattacaac ggaacaggaa tgtcagttat ggagatgagc
     1981 cacaggtcga aagcgtttga ggagatcatt aattcggcag aacaggattt gagagatctg
     2041 atgaatatac cggataatta taaggtgttg ttcatgcagg gaggagcgtc tcagcaattt
     2101 gctatgatcc cgatgaattt tatgaagaac agagtggctg attatattat tacaggacag
     2161 tgggcgaaaa aagcggcgaa agaggcagaa aaatacggga aggtaaatcg gattgcgtct
     2221 tcggaagatc agactttctc atatattccg gattgttccg acctgcctgt ttcggaagat
     2281 gcggattatg tatacatttg tgaaaacaat acgatttacg gaacgaagtt taaagaactt
     2341 ccgaacacaa agggaaaaac attgatcgct gatgtgtcgt cctgtttcct ttcagagccg
     2401 gttgatgtag aaaaatatgg aatgatctat ggcggagttc agaaaaatat aggacctgca
     2461 ggtgttgtaa ttgcgattat ccgtgaagat ctcattacag aagatgtgct tgaaggaacg
     2521 ccgacaatgc ttacatataa gacacatgct gatgcaaaat cgctttataa tacacctccg
     2581 gcatatggaa tttatatttg cggaaaagtg tttaaatggc tgaaagcaca ggggggactt
     2641 gctgcgatca aagagaaaaa tgagaaaaag gcgaaagtgc tttatgattt ccttgatcag
     2701 agtaaaatgt ttaaaggaac agttgtcaaa aaagatcgtt cccttatgaa tgttccgttt
     2761 gtaacaggag atgcagatct tgatgctaaa tttgtaaaag aagcgaaaga agcaggactt
     2821 gagaacttaa aaggacacag atcagtcggc ggaatgcgcg caagtattta caatgcgatg
     2881 ccgtatgaag gcgttgtggc tctggtggaa tttatgaaaa aatttgaaga ggagaatgca
     2941 taatcatgta taaatatcat tgcttaaatc caatttcggc agtaggactt gatcagctgg
     3001 atgaaaatta tgtgaatgtg gagactgcaa aaggagcaga cgctattctt gtgcgaagcg
     3061 caaagatgca cgagatggag tttgataaaa acttgaaagt gatcgcccgt gcgggcgcag
     3121 gtgtgaataa tataccgctt gaacgctgtg ccgaagaagg ggttgttgta ttcaatacgc
     3181 cgggagcaaa cgcaaacggc gtaaaagaac ttgtgattgc gggaatgctt ttggcagcaa
     3241 gagatattat cggaggaatc aactgggttc aggaatacga agaggatggc gatattgcaa
     3301 agatcacaga gaaaaagaaa aaagtattcg caggaacaga acttgaaggg aaaaaacttg
     3361 gcgttatagg tcttggcgcg ataggtgttc ttgtggcaaa tgcagccgtt catcttggaa
     3421 tggaagttta tggatatgat ccatatgttt ctgttgattc ggcatggaga ttgtcgcgca
     3481 atattcatca cgcgaaaaca gcagatgaaa tttataaaga gtgtgattat attacagttc
     3541 atgtgcctgc attggaagat acaaaaggta tgatcaacaa agacgctatc agtcttatga
     3601 aaaaaggcgt tgttattttg aattttgcca gagatgttct tgtgaatcag gaagacatcg
     3661 tagacgcgct tgtatcggag aaagtgcgct gctacgtaac ggacttcccg acaaaagaga
     3721 tagtcggtgt cagaggcgcg atcgtgattc cgcatcttgg tgcatctaca gaggagtcgg
     3781 aagataactg tgctaaaatg gctgcggcag aggtgaaaga ttttctcgag aatggaaata
     3841 tcacacattc tgtaaacttc ccggattgcg atatgggagc gaaaggagaa ggagagagaa
     3901 ttacaatcct tcataaaaat attccgaata tgatcggaca gttcaccgct cttttggctg
     3961 aaaagaatat gaatattgaa gttatgacaa ataaaagccg taaagaatac gcatatacga
     4021 tgcttgatgt ggatggaaca gtttcagaag atgtggaagc gcagttagca gcggtagaag
     4081 gcgtgttgaa agtaagagtg atcagataag tgaactctta ctgtgaagag agtaaaataa
     4141 attagatatc gttgagaaag agagtgtgaa tgacagaact gtttgcgctc tctttttgta
     4201 tgtaacagtc cggctataca gtaagggttc gtgaatagcg caggcagaat tgttactttt
     4261 gtataataaa atactttaat gaaggattga catattttgt atatttgtat acaattatac
     4321 aagaatacat atatctgagc acgaggtgca agagtatgta tagcatacat atatacgtgt
     4381 atacatataa aaagatgcaa tcataggagg aaatttgatg gaaaacagaa aagtattt
//
LOCUS       DBMDXF010000156         4398 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4762_length_4398_cov_1.897836, whole genome shotgun sequence.
ACCESSION   DBMDXF010000156 DBMDXF010000000
VERSION     DBMDXF010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_4398_cov_1.897836"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /gene="rimO"
                     /locus_tag="ACZPD4_06670"
     CDS             <1..1136
                     /gene="rimO"
                     /locus_tag="ACZPD4_06670"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1378778.1"
                     /translation="AEYKKSGQVKALIVTGCLAQRYRQEILDEIPEVDEVLGTTAYDK
                     ILDVVDRALEGEQGIILSDLNALPVSGAKRLVTTGGHFAYLKIAEGCDKHCTYCIIPK
                     IRGNFRSVPMERLIKEAKELAEQGVKELILVAQETTLYGKDLYGEKCLHRLVEELCKI
                     AGIRWIRILYCYPEEITDELIEVIKKEPKVCHYLDLPIQHASDSILKRMGRRTSKQEL
                     TDIVKKLRKEIPDICLRTTLITGFPGETEDQHEELMQFVDEMEFDRLGVFTYSPEEDT
                     PAAVMPDQIAEEVKEERQADLMELQQEIAFDNAENMIGREMLVMIEGKVADENAYVGR
                     TYRDAPNVDGLIFINTEEELISGDFAKVKITGALEYDLIGELL"
     gene            1133..1675
                     /gene="pgsA"
                     /locus_tag="ACZPD4_06675"
     CDS             1133..1675
                     /gene="pgsA"
                     /locus_tag="ACZPD4_06675"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1378779.1"
                     /translation="MNLPNKLTVLRIIMVPFFVFFMLTDVGGAANKWIALAIFAVASL
                     TDLLDGKIARKYNLVTNFGKFMDPLADKLLVCSAMICLIPSGKLAAEIVIIIIAREFI
                     ISGFRLVASDNGIVIAASYWGKFKTVFQMAMIIVLIADLGGVFDLIGEILIWAAVALT
                     IISLIDYVWKNKQVLTQGGM"
     gene            1678..2193
                     /locus_tag="ACZPD4_06680"
     CDS             1678..2193
                     /locus_tag="ACZPD4_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR1378780.1"
                     /translation="MYLEWYLKEKKAYEKTLEETVTELLSEKHLHVTTAESCTGGLIS
                     GTLVNAAGASAVLNEGYVTYSNEAKERLLGVSHETLETYGAVSEQTAKEMAEGAAKAA
                     GAEAALSATGIAGPSGGTEDKPVGLVYIGCYLNGETVVKECRFHGNRMENRAQTVETA
                     LEMLKDALLKE"
     gene            2388..3080
                     /locus_tag="ACZPD4_06685"
     CDS             2388..3080
                     /locus_tag="ACZPD4_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1378781.1"
                     /translation="MIYSHEVEMMCPVAQGANHGPAPIPEEAKWVKAKEVKDISGLTH
                     GVGWCAPQQGTCKLTLNVKDGIIQEALVETIGCSGMTHSAAMASEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEDGLPIGAGLEDLGKGLRSQVGTMYGTLAK
                     GPRYLEMAEGYVTGIALDENNEIIGYQFVSLGKFTDFIKAGDTPNDAWEKAKGQYGRV
                     DDAAKIIDPRKE"
     gene            3129..4139
                     /locus_tag="ACZPD4_06690"
     CDS             3129..4139
                     /locus_tag="ACZPD4_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIR1378782.1"
                     /translation="MALFESYERRIDKINEVLNSYGIASLEEAEKITKDAGLDVYNQI
                     KGIQPICFENACWAYITGAAIAIKKGCTRAADAAAAIGEGLQAFCIPGSVADQRKVGL
                     GHGNLGKMLLEEETDCFAFLAGHESFAAAEGAIGIAEKANKVRKKPLRVILNGLGKDA
                     AKIISRINGFTYVQTDYDYYTGELKEVSRTAYSDGLRSKVNCYGANDVREGVAIMWKE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECIEQGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTLGRMHSDAQFAGSSSVPAHVEMMGLIGAGNNPMVGMTVAVAVSIEEAAKEG
                     KF"
BASE COUNT         1404 a          761 c         1122 g         1111 t
ORIGIN      
        1 tggcagaata taaaaagtcg ggacaggtaa aagcgttgat cgtgacagga tgtctggcac
       61 agagatacag acaggaaata ttagatgaaa ttccggaagt agatgaagtt ctcggaacga
      121 cggcatatga taagatttta gatgtggtag accgagcatt ggaaggagag cagggcatta
      181 tactttcgga tcttaatgcg cttccggttt caggagcaaa gcgtcttgtg acgacgggag
      241 gacattttgc atatttaaag attgcggaag ggtgcgataa gcattgtaca tattgcatta
      301 ttccgaaaat acgtggaaat ttcagaagtg ttcccatgga acgactgatc aaagaagcga
      361 aagagctggc agagcaaggt gtcaaagaac tgatccttgt ggcacaggaa acgacacttt
      421 atggaaaaga tctgtatgga gaaaaatgtc ttcacaggct tgtggaagag ttatgcaaga
      481 ttgcgggaat ccgctggatc cgaattttgt attgctatcc ggaggaaatt acagatgaat
      541 tgatagaagt gataaaaaaa gaaccgaaag tgtgtcatta tcttgatctt ccgatccaac
      601 atgcaagcga cagtattttg aaaaggatgg gcagaagaac gtcaaaacag gaactgacag
      661 atattgtaaa aaaattgaga aaagaaattc ctgatatttg ccttcgtacg acgttgatca
      721 ccggctttcc cggcgaaaca gaagatcagc atgaagaatt gatgcagttt gtcgacgaaa
      781 tggagtttga tcgtttagga gtctttacct actcaccgga agaagacacc ccggcggctg
      841 ttatgccaga tcagatcgca gaagaagtaa aagaagaacg acaggcggat ctgatggaac
      901 ttcagcagga aattgcattt gacaatgcag aaaatatgat cggcagagaa atgcttgtta
      961 tgattgaagg caaagttgcg gatgaaaatg cttatgtcgg caggacatac cgcgatgcgc
     1021 cgaatgtgga cggattgatc tttattaaca cagaagaaga actgatatcc ggcgatttcg
     1081 caaaagtgaa aataaccgga gctttagaat atgatctgat aggagaattg ttatgaattt
     1141 accaaataaa ttaactgttt tgcgcattat catggtaccg ttttttgtat tttttatgct
     1201 tacggatgta ggcggagcgg ctaataagtg gattgctctc gcaatttttg cagttgcgag
     1261 tctgacggat cttttagatg ggaaaatagc gagaaaatat aatcttgtga caaatttcgg
     1321 aaaatttatg gatcctttgg cagataaact gcttgtttgt tcggctatga tctgtctgat
     1381 tccttccgga aaattagctg ccgaaattgt aataatcatt attgcaagag agtttattat
     1441 aagcggattc cgtctcgtgg catctgacaa cggaattgtg atcgcagcaa gctattgggg
     1501 aaaattcaaa actgtatttc agatggcgat gatcatcgtt cttattgcag atttaggcgg
     1561 tgtgttcgac ttgatcggag aaattcttat ttgggcagca gtggcactta cgatcatttc
     1621 tctgatcgat tacgtgtgga aaaataaaca ggtacttaca caaggtggga tgtaaacatg
     1681 tatcttgaat ggtatttaaa agaaaaaaaa gcatatgaga aaactttaga ggaaacggtg
     1741 acggaacttt tatctgaaaa acaccttcac gtgacgactg cagagtcctg tacaggagga
     1801 ctgatttcag gaacacttgt aaatgcggca ggcgcgtctg cagtattgaa tgaggggtat
     1861 gtcacttact caaatgaagc gaaagagcgc ctgcttggag tttctcatga gacgcttgaa
     1921 acttatgggg cagtcagtga acagacggcg aaagagatgg cggaaggagc ggcaaaagcc
     1981 gcaggtgcag aagcggctct tagcgcgaca ggaattgccg gtccaagtgg aggaacagag
     2041 gacaagcctg tcggactcgt gtatatagga tgttatctga acggggagac agtagtaaaa
     2101 gaatgccgtt ttcacggaaa tcgtatggaa aacagagctc aaacagttga aactgcactt
     2161 gagatgctga aagatgcatt gttaaaagaa taggattttc ggaaatagga aagaaaattt
     2221 taaaatagta taaaagcatt ataaaaacat aaagtgaaca ctttgagtga aaactttgtc
     2281 aaagtttgat tgacgaatgc ggtaatttat gagaaaataa aataggtatg gcacgagtat
     2341 gtgttcgtat gcagaagaaa atcatagata ttgaaaggag ttttaacatg atttattcac
     2401 atgaagtaga aatgatgtgt ccggtagcgc agggcgcaaa tcacggaccg gctccgatcc
     2461 cggaagaagc aaaatgggtg aaagcgaaag aagtaaagga tatttccggt ttgacacatg
     2521 gtgttggctg gtgtgctccg cagcagggta catgtaagct gactctgaac gtgaaagacg
     2581 gtattatcca ggaagcgctt gtagagacaa tcggatgttc gggaatgaca cattccgcag
     2641 ctatggcatc tgagatcctc ccgggcaaaa caatcttaga ggcacttaac acagaccttg
     2701 tctgtgatgc gatcaataca gcaatgagag aacttttcct tcagatcgta tacggaagaa
     2761 ctcagagtgc attctctgaa gacggacttc cgatcggagc aggacttgag gatcttggaa
     2821 aaggacttcg ttctcaggtt ggtacaatgt acggaacact tgcaaaaggt cctcgttacc
     2881 ttgagatggc agaaggttat gtaacaggaa tcgctcttga cgaaaacaac gagatcatcg
     2941 gataccagtt cgtaagcctc ggaaaattca cagatttcat caaagcaggc gatacgccga
     3001 acgatgcttg ggaaaaagca aaaggacagt acggccgtgt tgatgacgca gcgaagatca
     3061 tcgacccgcg taaggagtaa tcgggattta gcgacatatt aaggtaaata aatcaggagg
     3121 atacataaat ggctttattt gaatcatatg aaagaagaat tgataaaatc aatgaagttt
     3181 taaacagcta cggtatcgct tctcttgaag aagcagagaa gattacgaag gatgccggac
     3241 tggacgtata caaccagatc aaaggcattc agccgatctg ttttgaaaat gcatgctggg
     3301 cttatattac gggtgcggca atcgcaatca aaaaaggatg tacaagagca gcagacgcag
     3361 cagcagcaat cggagaggga cttcaggcat tctgtattcc gggttctgta gcagatcagc
     3421 gtaaagtagg tcttggtcac ggaaaccttg gaaagatgct tcttgaagag gaaacagact
     3481 gttttgcatt ccttgcagga catgagtcat tcgcagcagc agaaggtgca atcggtattg
     3541 cggaaaaagc aaacaaagtt cgtaaaaaac cgcttcgtgt tatcttgaac ggtcttggaa
     3601 aagatgctgc aaagatcatt tccagaatca acggatttac atatgttcag acagattatg
     3661 attattatac aggcgaactt aaagaagttt ccagaacagc ttactctgac ggacttcgtt
     3721 ctaaagtaaa ctgctacgga gcaaacgatg ttcgtgaagg cgttgcaatt atgtggaaag
     3781 agggtgttga tgtttctatt acaggaaact ctacaaaccc gactcgtttc caacacccgg
     3841 ttgcaggtac atacaaaaaa gaatgtatcg agcagggtaa aaaatatttc tcagttgctt
     3901 caggcggtgg tacaggacgt acacttcacc cggacaacat ggcagcagga ccggcttctt
     3961 acggtatgac agatacactt ggacgtatgc attcagatgc tcagttcgca ggatcttctt
     4021 ccgtaccggc tcatgttgag atgatgggac ttatcggagc aggaaacaac ccgatggttg
     4081 gtatgactgt agctgttgca gtttccatcg aggaagcagc aaaagaaggt aaattctaag
     4141 ataggtgcaa aaaaccttga ttttatgggg cttttcaagt cgtagtttgg cttgggaagc
     4201 tctttttgta tcggcaagac acatgtaagc cacacttgag tttatgatgg gttgcccctt
     4261 tttaaactag agattttgac atctccactc tacagagtta caatgccaga caatacaagg
     4321 gtagacaggc tcgttatatt tgactgaccc cattggaaac tgtatactag ccggtgtcaa
     4381 tggtgcaggt aagtcaac
//
LOCUS       DBMDXF010000157         4382 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4785_length_4382_cov_2.190142, whole genome shotgun sequence.
ACCESSION   DBMDXF010000157 DBMDXF010000000
VERSION     DBMDXF010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4785_length_4382_cov_2.190142"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            96..1379
                     /locus_tag="ACZPD4_06695"
     CDS             96..1379
                     /locus_tag="ACZPD4_06695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1378783.1"
                     /translation="MPSDKEQKKSPEEEAEEKKYSFLQEKIKGKQGSRETLIKQFIRI
                     AIYGLILGMFACLGFFALKPWMQQWFAGNAKTVMIPEDEQAAEDSEKNQEGKEDPALD
                     AESYEEMTAGLNERVKEARKSIVSVSSVSEKADWDGEMTGILQSVTGVIAGDNGREFL
                     IIADSSICTGASAWRVTISDGKSFDAALKKVDNNRKIAVFGIGKHVVDEDDSGLIKVA
                     VLGNSKKVAQGDAVIALGNIFGYADGMSYGVVSASNYKATFFDGECAVIATDIPAEEQ
                     GTGMLFNMDGEVVGMISSSVWNDTGKNVVNAYGISDLKPIIELLINGKSVPYIGIYGT
                     TVTEQLAEEHKMPSGIYVADVDPDSPAMAAGIQIGDIICEASGEEIKDIAAYQNIVIG
                     SSVGETMEFVGKRLGSDGYVNIKFSVVTTEKEEVK"
     gene            1674..2618
                     /locus_tag="ACZPD4_06700"
     CDS             1674..2618
                     /locus_tag="ACZPD4_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR1378784.1"
                     /translation="MRYISGLHEGETIRNIYLCKGKRSAETRNGKPYDNVLLQDKTGT
                     LDGKVWDPNSQGIADYDEKDFIEVYGEVINYNGNLQINIKQIRKAGEEEYNPADYMPT
                     TEKSVDGMYEQLTEFIRHISNPYLKSLLEYFFVNDQEFIKKFRSHSAAKTVHHGFSGG
                     LLEHTLSVLRMCEYFAGSYGILNKDLLLTAAMCHDIGKTRELSSFPDNDYTDEGQLIG
                     HIVIGVEMLDEAIRTIPDFPVRLANELKHCIIAHHGELEYGSPKKPALAEAVALNLAD
                     NADAKMQTLTEIFKGKTGTDWLGFNRLFDSNLRRTSGA"
     gene            2740..>4382
                     /gene="rlmD"
                     /locus_tag="ACZPD4_06705"
     CDS             2740..>4382
                     /gene="rlmD"
                     /locus_tag="ACZPD4_06705"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1378785.1"
                     /translation="MCMQKNDTVVVEITDIGVNGEGIGKIDGYTLFIKDAVIGDVAEV
                     KVIKAKKNYGYARLINVLEPSNSRVEPKCPFARKCGGCQIQEMSYEKQLEFKERKVRG
                     NLERIGGFSSELLECTKEPIIGMETPFYYRNKAQFPFGTDKEGNPVTGFYAGRTHDII
                     ANTNCILGAAVNEHILESILDFMKKYKIRSYDEKTGTGLFRHVLLRYGFTTKEIMVCF
                     VVNKDKADKSGEWFPHQKELVEELAKIDGMTSITASPNTKRTNVIMGDTFEVLWGQGY
                     ITDYIGSVKYQISPLSFYQVNPVQTEKLYSQALEYAGLKGDETVWDLYCGIGTISLFL
                     AQKAKQVYGVEIVPPAIDDARENALLNGIENAQFFVGKSEEILPKYYKDYEEQHGGEK
                     AHADVIVVDPPRKGCDAELLETILKMQPERIVYVSCDSATLARDLKILCEGAQEDEKT
                     AGYQIEKWRAVDQFPMTTHVETVVLLSQQKPDDTIEIDLDLDELDATSAELKATYQEI
                     KDYVLKEFGLKVSSLYISQVKRKCGIEVGENYNLPKSENA"
BASE COUNT         1540 a          670 c         1153 g         1019 t
ORIGIN      
        1 ttatccgtat aaagaaaagg actttcagga agtaagaaaa accttttcat agaggattga
       61 atcaggtata ctagaatttg gaggaaaata agtccatgcc gagtgataaa gaacaaaaaa
      121 aatcgcccga agaagaggcg gaagagaaga aatattcttt tttacaagaa aagataaaag
      181 ggaaacaggg cagcagggaa acgcttataa aacaatttat ccgaattgcg atctatggtc
      241 tgatattggg gatgtttgcc tgcttaggtt tttttgcgct taaaccgtgg atgcagcaat
      301 ggtttgcggg aaatgcaaag actgtgatga tccccgaaga tgaacaggca gcggaagatt
      361 ctgagaaaaa tcaagaggga aaagaagatc ctgcgctcga tgcggaaagt tatgaagaga
      421 tgaccgccgg gttgaatgaa cgtgtaaaag aggcgagaaa aagtattgtt tctgtatctt
      481 ctgtatctga aaaggcagac tgggacggag agatgacagg aattttgcag agtgttacag
      541 gtgttatcgc cggagataac ggtcgggaat ttttgattat cgccgacagt tcgatctgca
      601 caggcgcttc tgcatggaga gtgacaattt ccgatggaaa aagcttcgat gcagcgctga
      661 aaaaggtcga taataacaga aagatagcag tgttcggcat cgggaaacat gtcgtagatg
      721 aggatgacag cggattgatc aaagtagccg ttttaggtaa ttcaaagaaa gtggcgcagg
      781 gagatgcagt gatcgctctc ggaaatatat ttggatatgc agacggaatg tcttacggcg
      841 ttgtcagcgc atcgaattat aaggcgacat ttttcgacgg agaatgcgca gtgatcgcga
      901 cagacattcc ggcagaggaa caaggaacag gcatgttgtt taacatggat ggcgaagtgg
      961 tagggatgat ttcttcatcc gtatggaacg acacaggaaa aaatgttgtg aatgcatatg
     1021 gaatttcaga tttgaagccg attatagaat tgctcatcaa tgggaaaagc gttccatata
     1081 tcggaattta cggaacaacg gtgacggagc agcttgcaga agaacataaa atgccgtcag
     1141 gtatttatgt ggcagatgtt gatccggatt caccggcgat ggcggcggga atacaaatcg
     1201 gagatattat atgtgaagca tcgggagaag agataaagga tattgccgca taccaaaaca
     1261 ttgtgatagg ctcgtctgta ggagaaacga tggagtttgt cggaaaacgg ctcggttcag
     1321 acggatatgt caatataaaa ttttctgtcg tgaccacaga aaaagaagaa gttaaataaa
     1381 gaagcgggaa tgagaaaata agaaaagaag aagtttggta agaagcagga atgagaaaat
     1441 aggaaaagaa gaagttcagt aaaaagcggg aatgagaaaa taagaaaaga agaagttcag
     1501 taagaagcag gaatgagaaa ataagaaaag aagaagttca gtaagaagca ggaatgagaa
     1561 aataagaaaa gaagaagttc agtaaaaaag caggaatgaa aaaataagaa aacagattaa
     1621 aagaaataga acatagagaa ataaagaaaa aatagaaaga ggaatagcga gacatgagat
     1681 atatcagtgg gttacatgaa ggagaaacaa tcagaaatat ttatctgtgt aagggaaaac
     1741 gttcggctga aacaagaaac ggaaaaccat atgacaacgt gcttttgcag gataagacag
     1801 gaacgcttga cggaaaagta tgggacccga attcgcaggg gattgcggac tatgatgaaa
     1861 aagattttat agaagtatat ggagaagtga tcaattataa cggaaatctg cagattaata
     1921 taaaacagat ccgcaaagcc ggtgaagagg aatacaaccc ggcagattac atgcctacca
     1981 cagagaaaag cgttgacggg atgtatgaac agctgacaga gtttatccgt cacatttcca
     2041 atccgtatct gaaaagtctg ttagaatatt tctttgtgaa cgatcaggaa tttattaaaa
     2101 aattccggtc acattctgca gcgaaaacgg tacaccacgg attttcggga ggactgctgg
     2161 aacatacgtt gagcgtactt cggatgtgcg aatattttgc cggatcatat ggaattttaa
     2221 acaaagatct tctgttgaca gcggcaatgt gccatgacat cggaaaaaca agggaacttt
     2281 cgtcatttcc ggacaatgat tatacagatg aaggacagtt gatagggcat attgtgatcg
     2341 gagtggaaat gctcgacgag gcaatcagga caataccgga ttttccggtt cgccttgcaa
     2401 atgaattgaa acactgtatt atcgcccacc acggagagtt ggaatacggt tctccgaaaa
     2461 aaccggcgct tgcggaggcg gtagcgttga atcttgcgga taatgcagat gcaaaaatgc
     2521 agactttgac ggaaatattt aaagggaaaa caggtacaga ctggttagga tttaaccgat
     2581 tgttcgattc taatttgcgc agaacgtcag gagcgtaaac agaaaaagcc gattgtaggc
     2641 ggcggataga tcgtaactat acggtgtttc cggtggggaa atatgtgcgc agatgcggat
     2701 gtgcaaaaaa tgcaggaaaa cagaaagaaa agggaagata tgtgtatgca gaaaaatgat
     2761 actgtagttg ttgaaatcac ggatatcggt gtcaacggag aaggaatcgg aaagatagac
     2821 ggatatacgc tgttcataaa agacgctgta ataggagatg ttgcagaagt taaagtgatc
     2881 aaggctaaga aaaattacgg atatgcccga ttgataaacg tgcttgaacc gtcaaacagc
     2941 cgggttgaac ctaagtgtcc tttcgcaaga aaatgcgggg gttgtcagat tcaggaaatg
     3001 tcctatgaaa agcagcttga gtttaaagaa agaaaagtca ggggaaatct tgaacggatc
     3061 ggcggttttt catctgaact tttggaatgt acaaaagagc cgatcattgg gatggaaact
     3121 ccgttttatt atagaaataa ggcgcagttt ccgtttggaa cagacaaaga aggaaatccc
     3181 gtaacaggat tttacgcagg ccgtacacat gacattatag caaatacaaa ctgcattctc
     3241 ggtgcagcgg taaatgaaca tattttagag agcattcttg attttatgaa aaaatataag
     3301 atccgttcct atgatgaaaa aacaggaacg ggtctgtttc gtcatgtgct gcttcgttat
     3361 ggatttacga caaaagaaat catggtgtgt tttgttgtga ataaagataa agcggataaa
     3421 agcggagaat ggtttccgca tcagaaagag ttggtggaag agcttgctaa aattgacgga
     3481 atgacgagta taacggcaag tcccaacaca aagcgaacaa acgtaattat gggagatacg
     3541 tttgaggtgc tttgggggca aggatatatt acagactata tcggctcagt gaaatatcag
     3601 atttctcctt tgtcgtttta tcaagtaaac ccggtacaga cggaaaaatt atactctcag
     3661 gcgcttgaat atgcaggctt aaaaggagat gaaacggttt gggatttgta ctgcggtatc
     3721 ggaacaattt ctttgtttct tgcgcagaag gcaaaacagg tttacggcgt ggaaatcgtg
     3781 ccccctgcga ttgatgatgc aagagaaaac gcactgttaa acggaataga aaacgcacag
     3841 ttttttgtgg gaaaatctga agaaatcctt ccgaaatact ataaagatta tgaagaacag
     3901 cacggaggag aaaaggcaca cgcggatgtg atcgtcgtag atccgcctcg gaaagggtgc
     3961 gacgctgaat tgttggaaac gattcttaag atgcagccgg agaggattgt ttatgtaagc
     4021 tgcgattccg caacattggc aagagatctg aaaatattgt gcgaaggggc acaggaagac
     4081 gaaaaaacag cagggtatca gattgagaaa tggagagcgg ttgatcaatt tccgatgacg
     4141 acacatgtgg agacggttgt tcttttgtcc caacaaaaac cagatgacac gatagagatc
     4201 gacttagacc tggacgagct ggatgccacc agtgccgagt tgaaagcaac ctatcaggaa
     4261 atcaaagatt atgtgctgaa agaatttggc ttgaaggttt caagtttata tatttctcag
     4321 gtaaaacgca aatgtggaat tgaagtggga gaaaactata atcttccaaa atcagaaaat
     4381 gc
//
LOCUS       DBMDXF010000158         4365 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4804_length_4365_cov_1.852955, whole genome shotgun sequence.
ACCESSION   DBMDXF010000158 DBMDXF010000000
VERSION     DBMDXF010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4804_length_4365_cov_1.852955"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="ACZPD4_06710"
     CDS             complement(<1..1071)
                     /locus_tag="ACZPD4_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HIR1378786.1"
                     /translation="MDINQKLTEELDVNRWQIDAAVTLIDEGNTIPFISRYRKEATGS
                     LNDEQLRKLHERLLYLRNLEEKKEQVISSIEEQGKMTEELKEKILLAETLVTVEDLYR
                     PYRPKRRTRATIAKEKGLEPLAAYMMLQQAKEPLEETAKQYISEEKEVKTEEEAIAGA
                     KDIIAEIISDNADYRTWIRKTTMKKGKVVSTAKDPETESVYEMYYEFEESVEKLAGHR
                     ILALNRGEKEKILTVKVEAPEEDILRYLEKKTIVNDNPYTTSVLKEVVEDSYKRLIEP
                     AIEREIRNELTEKAEDGAIDVFGKNLHQLLMQPPIAGKVVLGWDPAFRTGCKLAVVDP
                     TGKVIGTTVIYPTAPTTPQKIKA"
     gene            1312..2199
                     /locus_tag="ACZPD4_06715"
     CDS             1312..2199
                     /locus_tag="ACZPD4_06715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1378787.1"
                     /translation="MERTITYKITDSGQNIRIDSFLRKHGYSMQNLTLLKKMPESILK
                     NGEWSYMQTTLQAGDILTVRIQEETSSPNIPAVNLPIDIVYEDEDIIVINKAAGMPIH
                     PSLNNYRNSLANALMWYYEQQNKPFIFRCTNRLDRDTSGLTVVAKHMVSSNILSSMTA
                     RHQIEREYLAIVRGHVTPFEGTINAPIGRTGSSLIERKIDFENGEHAITHYHTVFEKN
                     GLSLVSLILETGRTHQIRVHMKHLGFPLIGDYLYNPDMELIDRQALHSHCLRFLHPIT
                     GRQMTFTADLPEDMARLFA"
     gene            2310..2383
                     /locus_tag="ACZPD4_06720"
     tRNA            2310..2383
                     /locus_tag="ACZPD4_06720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2344..2346,aa:Arg,seq:acg)
     gene            complement(2513..3598)
                     /gene="trpS"
                     /locus_tag="ACZPD4_06725"
     CDS             complement(2513..3598)
                     /gene="trpS"
                     /locus_tag="ACZPD4_06725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1378788.1"
                     /translation="MRKVMLTGDRPTGRLHVGHYVGSLRRRVELQNTGEFDDIFIMIA
                     DAQALTDNADNPEKVRSNIIEVALDYLACGLDPEKSTLFIQSQIPQLCELSFYYMNLV
                     TVSRLQRNPTVKTEIKMRNFETSIPVGFFTYPISQAADITAFKATTVPVGEDQMPMIE
                     QTKEIVHKFNSVYGDTLIDPKILLPENQACLRLPGIDGKAKMSKSLGNCIYLSEEPEE
                     IKKKVFSMFTDPTHIRVEDPGKLEGNTVFTYLDAFCRPEYFAEFLPEYSDLDELKAHY
                     QRGGLGDMKVKRFLNNVLQAELEPIRNRRKEFSKDIPAVYEMLKKGSEKAEAVAAQTL
                     AEVKAAMKINYFDDTELIKAQSEQFAE"
     gene            complement(3697..4356)
                     /locus_tag="ACZPD4_06730"
     CDS             complement(3697..4356)
                     /locus_tag="ACZPD4_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1378789.1"
                     /translation="MSLSLFLIIFIYMPWFLLLEKHVTTNYHVIQTQFDMWIPFVEYF
                     IIPYLMWFAFIAAAFVYFFFTDVPGFYKMAKFMFTGMTIFLIISTIFPNGQDLRPVVF
                     ERDNVFVDMVRMLYRADTCTNVFPSLHVFNTLSVCIAVHESEKLKKHKAVCNAAYILA
                     ALIILATMFLKQHSVLDVFGAVIMAYVLYKVVYEPEKKMIPQLSKQKFSKQKRIAAHN
                     K"
BASE COUNT         1171 a         1104 c          770 g         1320 t
ORIGIN      
        1 tgccttgatc ttctgcggag tcgtcggagc cgtcggataa atgaccgtag ttccgatcac
       61 ctttcccgtc ggatcgacaa ctgccagttt acatcccgta cgaaacgccg gatcccatcc
      121 aagcactact ttccccgcga tcggcggctg catgagaagc tgatgcagat ttttcccgaa
      181 tacgtcgatt gctccgtctt ccgctttctc cgtcagttca tttcttatct ccctttcgat
      241 cgcaggctcg atcagacgtt tataactgtc ctcgacaact tcttttaaga ccgaagtcgt
      301 atacggatta tcatttacaa tcgtcttttt ctctaaatat ctcagaatat cttcctccgg
      361 agcctctact ttgaccgtca gtattttttc tttctcaccg cggttcagcg caagaatccg
      421 atgtccggca agcttttcaa ccgattcttc aaattcgtaa tacatctcgt aaacagattc
      481 tgtctccgga tcttttgctg tagaaacgac ttttcctttt ttcatcgtcg tttttctgat
      541 ccatgtacga taatccgcat tatcggaaat gatttccgca ataatgtctt ttgctccggc
      601 gattgcttct tcctctgtct ttacttcttt ttcttccgaa atatattgct ttgcggtttc
      661 ttccaaaggt tcttttgcct gctgcagcat catatacgct gccaaaggct ctaatccttt
      721 ttctttcgcg atcgttgccc tcgtccgccg cttcggacga tatggccgat acagatcttc
      781 cactgtcacg agcgtctcag ctaaaaggat cttctctttg agttcttctg tcatctttcc
      841 ctgctcttca atgctcgaga tcacctgctc tttcttctct tccagatttc tcagatagag
      901 aagtctttca tgaagcttcc taagctgctc gtcgttgagt gacccggtcg cttcttttct
      961 atatctcgat ataaacggga tcgtattccc ttcatcgatc aaagttaccg ccgcgtcaat
     1021 ctgccagcgg ttcacatcaa gttcttctgt cagtttttga ttgatatcca tttataaaaa
     1081 tcctctctct aaattaattc acaaacagca ttatacatag tttaccacac acgtacattc
     1141 cattccacct tttcccgcga actttacacg actgctttac attattgacc ggtcttgtct
     1201 gtctttgtta taatatatga ctgaactgac gcaatacaaa aacagacatg cgaaacccgg
     1261 catataaaaa ttgccgcctc gttttgcgcg ccgaaacgaa atggagaagt tatggaacgt
     1321 acgatcacat ataaaataac cgacagcgga caaaacatcc gtattgacag ttttctccga
     1381 aagcacggat attccatgca aaatctgaca cttcttaaaa aaatgcccga aagcatttta
     1441 aaaaacggag aatggtctta tatgcaaaca acacttcagg caggagatat tctgactgtc
     1501 cgcattcagg aagaaacttc ctctccgaat attcccgctg tcaatctgcc gatcgatatt
     1561 gtctatgaag atgaagatat catagtgata aacaaagctg ccggaatgcc gatccaccct
     1621 tctcttaaca actatcgaaa ctctcttgca aacgcgctga tgtggtatta tgaacaacaa
     1681 aacaaacctt ttattttccg ctgtacaaac cgtcttgacc gggacacttc cggtctgact
     1741 gtcgttgcga agcatatggt cagctctaat atcttatctt ccatgaccgc ccggcatcag
     1801 atcgaacggg aatatctcgc cattgtacgc ggacacgtca ctccttttga aggaacgata
     1861 aatgctccga tcggacgaac cggaagttct ctcatcgaac ggaagatcga ttttgaaaac
     1921 ggcgaacatg caattaccca ttaccacaca gtttttgaaa aaaacgggct ttcactcgtt
     1981 tctcttattt tagaaacagg ccgcacacat cagatccgag tccatatgaa acacctcgga
     2041 tttcctttga tcggagatta tctgtacaat ccggatatgg aactgatcga ccggcaggcg
     2101 cttcactctc actgtcttcg ctttctccat ccgataaccg gaagacaaat gacatttacc
     2161 gccgatctgc ccgaagacat ggcaagattg tttgcctgag aaccccttga aaatcattct
     2221 ttaaaattat tcatttttta ttccaaaaag ggttgacata tttcaaaacc tataatataa
     2281 taaatttcac tgtgaacgaa acagcaaatg tgcgattagc tcagctggat agagcgtctg
     2341 gctacggacc agaaggtcgg gagttcgaat cttctatcgc acgtacgaaa agagatgttg
     2401 cttatgcagc atctttttta ttgtaaaata attcttgatc aggctcggat tttccccgtc
     2461 gccgacaaaa aaactcagaa gaactttctc ctgagttttt tgatatcatt tcttattctg
     2521 caaactgttc tgactgcgct ttaatcagtt ctgtatcgtc aaagtaattg atcttcattg
     2581 cagcttttac ttctgcaagt gtctgtgccg caaccgcttc cgctttctcg cttccctttt
     2641 taagcatctc ataaaccgcc ggaatatctt tactgaattc ttttctgcgg ttcctgatcg
     2701 gctctaactc ggcctgaagt acattattca gaaaacgttt cactttcata tctccgagtc
     2761 cgccgcgctg ataatgtgct ttcagctcgt ccagatccga atattccggc aaaaactctg
     2821 caaaatattc cggtctgcaa aatgcatcaa gataagtaaa tactgtgttt ccttccagtt
     2881 ttcccggatc ttccacccgg atatgagtcg gatcggtaaa catactgaac actttcttct
     2941 taatctcctc cggctcttcc gacagataga tacagttccc gagcgatttg ctcattttcg
     3001 cttttccgtc aattcccgga agccgcagac atgcctgatt ttcagggaga aggatcttcg
     3061 gatctataag tgtatcccca tatacggaat taaacttatg aacgatctct tttgtctgtt
     3121 cgatcatcgg catctgatct tcgccgaccg gaacagtcgt cgccttaaac gccgtaatat
     3181 ccgccgcctg actgatcgga tatgtgaaaa atcctaccgg aatactcgtc tcgaaattac
     3241 gcattttaat ctctgttttt acagtagggt ttctctgaag tcttgacacc gtcacaagat
     3301 tcatataata aaacgacagt tcacataact gcggaatctg cgactggata aaaagcgtcg
     3361 atttttccgg atcaagtccg cacgcaagat aatccaaagc gacttcaata atattgctgc
     3421 gcaccttttc cggattatcc gcattatccg tcagcgcctg agcatctgcg atcataataa
     3481 aaatatcgtc gaactctccc gtattctgaa gctccacacg tcttcttaac gatcctacat
     3541 agtgtcccac atgaagtctt cctgtcggac ggtcacctgt aagcataact tttctcataa
     3601 tgttcattcc ctctcatttc ccattttcgc tcctgtcgaa aatgttcctg ttaaaattaa
     3661 tctatcttta tcctgtttat ctttaccgct gctcttttat ttattatgag cggcaatccg
     3721 cttctgcttt gaaaattttt gttttgagag ctgaggtatc atcttctttt ccggctcata
     3781 gacgacttta taaagtacat acgccataat caccgctccg aatacgtcca gaacagaatg
     3841 ttgtttcaaa aacatcgttg caagaatgat cagcgccgcc aatatgtacg ccgcattgca
     3901 gacagctttg tgttttttca gtttttcact ctcatggaca gcgatacaca cgctcagcgt
     3961 attaaataca tgcagactcg ggaatacatt cgtacatgta tccgcacgat acagcatccg
     4021 caccatatcc acaaatacat tatctctctc aaatacgacc ggacgaagat cctgcccatt
     4081 cggaaatatc gtcgatatga tcaagaatat tgtcataccg gtaaacataa acttcgccat
     4141 tttataaaat cccggtacgt ccgtaaagaa aaaatacaca aatgcagccg cgataaacgc
     4201 aaaccacatc aaatacggaa taataaaata ttcaacaaac ggaatccaca tatcaaactg
     4261 cgtctggatc acatgatagt ttgtcgttac atgcttttcc aaaagcaaaa accacggcat
     4321 gtagataaaa attataagaa aaagtgaaag cgacaggctt tcggg
//
LOCUS       DBMDXF010000159         4352 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4824_length_4352_cov_1.973183, whole genome shotgun sequence.
ACCESSION   DBMDXF010000159 DBMDXF010000000
VERSION     DBMDXF010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4824_length_4352_cov_1.973183"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(382..756)
                     /gene="rplL"
                     /locus_tag="ACZPD4_06735"
     CDS             complement(382..756)
                     /gene="rplL"
                     /locus_tag="ACZPD4_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR1378790.1"
                     /translation="MAKMTTAEMIEAIKELSVLELNELVKACEEEFGVSAAAGVVVAA
                     AADGGAAAEEKDEFDVELVSAGASKVKVIKAVREITGLGLKEAKELVDNAPKTLKEGV
                     SKAEAEEIKAKLEAEGAEVSMK"
     gene            complement(865..1395)
                     /gene="rplJ"
                     /locus_tag="ACZPD4_06740"
     CDS             complement(865..1395)
                     /gene="rplJ"
                     /locus_tag="ACZPD4_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1378791.1"
                     /translation="MAKVELKQPIVAAIAEDIKDAQSVVIVDYRGLTVAQDTELRKKL
                     REAGVTYKVCKNTMMKRAFEGTEFAGLDEYLEGPSALCVSKEDATAPARILAEFAKTA
                     DKLEMKAGVIEGTVYDVAGLNELAKIPSREVLLSKLLGSLQSPITNFARVIKQIAEKD
                     GEAATEAPAEEAAAEE"
     misc_feature    complement(1529..1671)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1680..2447)
                     /locus_tag="ACZPD4_06745"
     CDS             complement(1680..2447)
                     /locus_tag="ACZPD4_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378792.1"
                     /translation="MKNKRIHRKYKDRVFRIIFRDKKELPELYNVVNDSAYTNPDELT
                     ITTIEDVVYMGMKNDLSFIIGDVMNLYEHQSSYSPNLPLRGLFYFASLYRERIEDVKQ
                     KLYMNVPLHIPFPQYIVFYNGTKQEPERQELKLSDLFVKTGKEVPPALECKAVVLNIN
                     FGHNRELMEKCVTLREYSRFVAMIRQQMSGELPFEEAVERAVDTCIRQGILSEILRKN
                     RSEVIDMILTEYNEEEFSLSEGEAEQYVKKVLDNLDE"
     gene            complement(2932..>4352)
                     /locus_tag="ACZPD4_06750"
     CDS             complement(2932..>4352)
                     /locus_tag="ACZPD4_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1378793.1"
                     /translation="EGISISTLLLPLQRIGSKTNSVKAVIDNVVVSTEVPETTKHDKS
                     GWTGKATSAHANVGNEGTFEMAFDSKPETHWHSDYSGNKEDKLLKDNLNKVKGKLDSV
                     TGEITFDKGYEINKVSFTPRTNAASGIITKADLFVKNSADGEWIPVAENQTFANNSSK
                     KTVRFAPQTVWGIKIVAKQSNDGWVAVSEFDIAVPQKCDVVVEAQEGGKVEGAVKDAN
                     EGSEVTVKATAKKGYDFTGWYDMTTGQKVSDDAEYTFTVEMNTSLIAHFAKNGETPDP
                     QLKEWTVTFKGSDGEIIAGVKVEDGKKVARPENDPVKEGYKFIGWFVDGKEFDFNTEI
                     KGNLVIEARFEKNDTPTPQPGEWTVTINGQTVTVKDGETLARPADPVKEGYKFIGWFV
                     DGKEFDFNTPITGDLKIEARFEKLPTPEPNPDKNDKNDKNDKVEGAVQTGDTANAVPW
                     IVCLGAAAGAGVVVLKSRKKRS"
BASE COUNT         1095 a         1090 c          712 g         1455 t
ORIGIN      
        1 gggacttctc ttgcatctca attattcccg ttatcgtggc agtcgccaaa acaccgtata
       61 aacagagatt ttggcacgtt gcttcgcgta aatgaaaaga gccttgtaat agtaataacg
      121 cttctaatac ggcatacctt tccataatag tgcattttta actataacac aaagctcttt
      181 tcgttgtcaa taacatttcc catttttttc ttgacatttt tcttcatata tgatattatg
      241 cgtcactttt cgacaaacgg cgatgtttaa cgtaaacgca caacaaagac aaaagaaccg
      301 cagaagcatt ttatcttctg cggttgattt tcttaagacc gtcttactta aagacgttca
      361 taaagttatc ggaaactccg attatttcat gctaacttct gcaccttctg cctcaagttt
      421 agctttgatc tcctcagcct ctgctttgga aacaccctct ttgagtgtct tcggagcgtt
      481 gtcaacaagc tcttttgctt ctttcagtcc aagacctgtg atctcgcgaa ctgctttgat
      541 aactttaact ttggaagctc ctgcagatac gagttctacg tcaaactcat ctttttcttc
      601 tgctgctgcg ccaccgtctg ctgctgctgc aactacaacg cctgctgctg cagatacacc
      661 aaactcttct tcacatgctt ttactaattc atttaactct aatactgata actctttgat
      721 cgcttcgatc atttcagctg ttgtcatttt agccattttt gatatcctcc atttttaaat
      781 ttactctgcc ggactcttct tctgatctcc ggttcagatt ttgttccttt ttacattgcg
      841 ccgcatatcc ggcacatctg tattttactc ctctgctgcc gcttcttcgg ccggagcctc
      901 tgttgctgcc tcgccgtctt tctcagcgat ctgtttaata acacgagcaa agtttgtaat
      961 cggtgactgc aagcttccga gcaatttgga gagaagtacc tctcttgacg gaatcttagc
     1021 cagttcattc aatccggcta catcatatac agttccttca attacaccgg ctttcatctc
     1081 aagtttgtct gctgtcttag caaactctgc aagaattctg gccggagctg tcgcatcttc
     1141 cttagatacg caaagtgcgc tcggtccttc taaatactca tccagacccg cgaactctgt
     1201 tccttcaaat gctctcttca tcattgtgtt cttacatact ttgtaagtaa cgccggcttc
     1261 tctgagcttt ttacgaagct ctgtatcctg tgctactgta agtccacggt agtcaacgat
     1321 aactacagac tgagcatctt tgatgtcttc tgcgatagct gctacgatcg gttgttttaa
     1381 ttcaactttt gccacgtcca ggtgcacctc cttatagatt ttaaatgact gcgtcctgag
     1441 accttcaatc atcggaagcg ccgaaaggca catcccggga ttgcaggcga ttaccaacat
     1501 cccgggcaac ccccgaactt tggctcgtcg cctatacaaa aaacctctgc gacagaagac
     1561 acagaggttt acaaatgttc tttttaaaaa gaattcgtat cttcctcggc aggcgttctc
     1621 aaccttatgc ctaacggcac ctgctgtctt cggcagtcaa ttacttttat aaaatatcac
     1681 tactcatcca aattgtcaag cacttttttc acatattgtt ccgcttcacc ttccgataaa
     1741 gaaaactctt cctcattata ttccgtcaaa atcatatcga ttacctccga ccgattttta
     1801 cgaagtattt cagacaaaat tccctgacgg atgcaagtgt ctaccgcccg ctctaccgcc
     1861 tcttcaaacg gaagttctcc tgacatctgc tgacggatca ttgcgacaaa ccgggaatat
     1921 tcccgcagtg tcacacactt ttccataagt tctctgttgt gaccaaaatt aatattcagc
     1981 acaaccgctt tacattcaag cgccggcggc acttcttttc ccgtcttaac aaacaagtcg
     2041 cttagtttca gttcctgtcg ttccggctct tgtttcgttc cgttataaaa aacaatatac
     2101 tgcgggaacg ggatatgtaa aggaacgttc atatagagct tttgtttcac atcttcgatc
     2161 cgttcccgat aaagcgacgc aaagtaaaac aatcctctca gcggcagatt tggactatat
     2221 gaactttgat gttcatataa attcatcaca tcaccgataa taaaggacaa gtcattcttc
     2281 attcccatgt acacaacatc ttcaatcgtt gtgatcgtca gttcatcggg attcgtatac
     2341 gcgctgtcat taaccacgtt atacagctcc ggaagttcct ttttatctcg aaaaataata
     2401 cggaaaaccc tgtctttata cttcctgtgt attcttttat ttttcaaccg atttcctcct
     2461 tcattgtagc aaacataatt atatgaagca atcaaaaata cgcgctttcc tcagcgcctt
     2521 tatccctttt tcttttctca tgttttttac aaatttgatt ttcactgcag cgactgccgc
     2581 aaaattatta gaattcaacg aaataaaacc gggtcttttt cttcccgcat tctcagagaa
     2641 ctgaatttat gataaaagaa taacacatct tctatttttc tacaaacatt ttatcttttt
     2701 tcaatgacag aattcctgtc attaattcac aatcaccgga agtgccaaaa ggcacattcc
     2761 gggattgcaa gcagtcgcca aggtctcggg caagcccgga ctttggctca ttgctattac
     2821 aaaaactgcc ggcaggaatt acctgccggc agaaaaatct gtattttact aattttattt
     2881 tttctgtttt gaagatcaat catttctcaa tctccaaaaa cattttttga atcaggaacg
     2941 ttttttgcgt gatttcagaa ctacaacgcc agctcctgct gctgcgccga ggcatacgat
     3001 ccacggtact gcatttgctg tatcgcctgt ctgaactgca ccttctactt tatcattttt
     3061 atcatttttg tcgtttttgt caggatttgg ttctggtgta ggaagcttct caaaacgcgc
     3121 ttcgatcttc agatctcctg tgatcggtgt gttaaaatcg aattcttttc catccacaaa
     3181 ccaaccgatg aatttgtagc cttcttttac cggatcagca ggacgggcaa gtgtctctcc
     3241 gtcttttact gtaactgttt gtccattgat cgtaactgtc cattctcccg gctgcggagt
     3301 cggtgtatca ttcttctcga aacgagcttc aattacaaga tttcctttga tctctgtatt
     3361 gaaatcgaat tcttttccat ccacaaacca gccgatgaat ttgtagcctt ctttcacagg
     3421 atcgttttcc ggacgagcca ctttctttcc atcttctact ttaactcctg caattatctc
     3481 tccgtcagaa cctttaaatg ttactgtcca ctctttcagc tgcggatctg gtgtctctcc
     3541 atttttcgca aaatgagcga tcaaagatgt attcatctct acagtaaatg tatattctgc
     3601 atcgtcagac actttctgac ccgttgtcat atcataccat ccggtgaaat cgtaaccttt
     3661 cttagcagtt gcttttactg taacttccga tccttcattt gcatctttta cagcaccttc
     3721 aacttttccg ccttcctgtg cttctacaac aacatcacat ttctgtggta ctgcaatatc
     3781 aaattcagat acagctaccc aaccatcatt tgactgtttt gccacaattt taattcccca
     3841 tacagtctgc ggagcaaaac gaacagtctt tttagacgaa ttgttcgcaa atgtctgatt
     3901 ctctgcaacc ggaatccact ctccatcagc agaattttta acaaacaaat ctgcttttgt
     3961 tataataccg ctcgccgcat ttgttcttgg cgtaaatgaa accttattga tctcataacc
     4021 tttatcaaaa gtaatctcac ctgttacact gtcaagtttt cctttaactt tattcaagtt
     4081 atctttcaac aacttatctt ctttgtttcc gctgtaatct gaatgccagt gtgtttccgg
     4141 ttttgagtca aacgccatct cgaacgttcc ttcatttccg acatttgcat gagcagatgt
     4201 cgctttacct gtccatccgg atttatcatg ttttgtcgtc tccggtactt ctgtagaaac
     4261 aacgacatta tcaattaccg ctttaacgga attagtcttt gaaccgattc tctgaagtgg
     4321 aagcagaagt gtagagatag aaattccttc tt
//
LOCUS       DBMDXF010000160         4295 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4883_length_4295_cov_1.840010, whole genome shotgun sequence.
ACCESSION   DBMDXF010000160 DBMDXF010000000
VERSION     DBMDXF010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_4295_cov_1.840010"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(37..564)
                     /locus_tag="ACZPD4_06755"
     CDS             complement(37..564)
                     /locus_tag="ACZPD4_06755"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HIR1378794.1"
                     /translation="MRWIDMHCDTVSEIYKNRKGTLMKNTLCVDMERLERAGAQMQFF
                     ACFVNLKDYRTDLRYADEKKTKNPGIYIQNADGTGEDKESEDRAYKAVRRMISYAKEE
                     IDRCNQADRKIKQQNTCFLNPVLTVEEGGVLGGKTERIDELYEEGVRLMTLTWNYENC
                     IGSPNSANRKVMSLV"
     gene            698..1429
                     /locus_tag="ACZPD4_06760"
     CDS             698..1429
                     /locus_tag="ACZPD4_06760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1378795.1"
                     /translation="MHPKIGIIICGFNENRQFVPNVYIQSVRYAKGIPLLIPLVRSDR
                     MIDDYVSLCDGFLFCGGEDITPLLFGEEPQNGNGKTDITVDLFQIRLMKRVLASRKPV
                     LAICRGMQILNVSCGGTIWQDLSLIPQPTLNHTQQSASRGDVSHRIKIERCSKLKQFV
                     GSCIYVNSFHHQSVKTPGRNVCVSARSPDGVIEAIELSGHPFALGVQWHPECMYRTSP
                     EMRSLFHEFIAHSLINSKDSGHTIS"
     gene            1458..1640
                     /locus_tag="ACZPD4_06765"
     CDS             1458..1640
                     /locus_tag="ACZPD4_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378796.1"
                     /translation="MTEISILDLQNLRHLIGGYSTTHCKMTDYASKAEDPEIKKLFQE
                     AADSAMKNKQELMKFL"
     gene            1699..>1876
                     /locus_tag="ACZPD4_06770"
     CDS             1699..>1876
                     /locus_tag="ACZPD4_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HIR1378797.1"
                     /translation="MDEKTLVSDALVGINGELKMFGDMIPQTENKELKQCLKQIRNQC
                     EMAQEKMYTAAREKS"
     assembly_gap    1877..1886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1887..1950
                     /locus_tag="ACZPD4_06775"
     CDS             <1887..1950
                     /locus_tag="ACZPD4_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HIR1378798.1"
                     /translation="PAEKASMQEIEHVKSILNVK"
     gene            complement(2018..2860)
                     /locus_tag="ACZPD4_06780"
     CDS             complement(2018..2860)
                     /locus_tag="ACZPD4_06780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1378799.1"
                     /translation="MIKLLAIDMDGTCLDQRSRMTDRTLDVLRKAAKAGITVVPCTGR
                     NLGCIPHRLAAGVLRESKTEDDEKNKDLFRYVITSNGAMVTDVKEKKTLFRALIKKED
                     ALSILSDCRKEKFGIAAHVRHRYFAQGKLFTSAGRIVYGKDAAAVCCVRNMEEILSKS
                     DCDVEELQFYFPTSKEKEKLKEILSVYPQVKAAYTGIYAEIFSKDASKGNGLKALSAH
                     LGIEKEEIACIGDGENDSFMFEESGLKIAMGNAEEMLKQRADYVTSSNRHDGAAEAIE
                     KYIL"
     gene            complement(2867..4240)
                     /locus_tag="ACZPD4_06785"
     CDS             complement(2867..4240)
                     /locus_tag="ACZPD4_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1378800.1"
                     /translation="MSQYDVIIIGAGPGGIFAAYELAKKAPELKVAVFEAGNSLEKRK
                     CPIDGKKIKSCINCKTCAIMSGFGGAGAFSDGKYNITNDFGGTLYEYIGKDTAIDLMK
                     YVDEINLAYGGEGTKMYSTAGTDIKKLCIQNELKLLDASVRHLGTDINYIVLENLYKE
                     LKEKVEFRFNYRVDELEAVDGGYRVISGDSTDECEECIISVGRSGSKWMEKVCESLDI
                     ETNSNRVDLGVRVELPAVIFSHLTDELYESKIVYRTEKFEDKVRTFCMNPYGIVVNEN
                     TNGIVTVNGHSYEDPKKQTENTNFALLVAKHFSEPFKDSNGYGESIARLSNMLGGGVI
                     VQRFGDLTRGRRSTAKRIEEGFVRPTLSAEPGDLSLVLPKRILDGIIEMIYALDKIAP
                     GTANDDTLLYGVEVKFYNMEVKLDEHLETNHKGLYVIGDGSGVTHSLSHASASGVYVA
                     RSIAKRK"
BASE COUNT         1155 a         1104 c          708 g         1318 t
ORIGIN      
        1 caacaaggtc cctttaatgg tggtttctcc atagcttcag accaaactca tcacttttct
       61 attagcactg tttggactgc cgatacaatt ttcataattc catgtcaacg tcatcagacg
      121 gacgccttct tcatacaatt catcgatccg ctctgttttt cctccaagga caccgccttc
      181 ttctaccgtc agcacagggt tcaaaaaaca tgtattttgt tgttttattt tccgatccgc
      241 ctgattacat ctgtctattt cttccttggc atatgagatc atccggcgaa ccgccttata
      301 tgctctgtct tcactttcct tatcttcacc tgttccatct gcattttgta tatagatacc
      361 cggattcttt gttttctttt catctgcata ccgcagatct gttctgtaat cttttaaatt
      421 tacaaaacac gcaaaaaact gcatctgcgc tcccgctctt tcaagccgtt ccatatctac
      481 gcacaatgta tttttcatca gcgttccttt tctgttttta taaatctcgc tcaccgtatc
      541 gcagtgcata tcgatccatc tcatagcttt tcctcttttc aaatttttat tttcttctct
      601 tatgtataag cagttctctt tttacatatt ctgactatag cacaagcgca gtacaatcgg
      661 cggccttctg caaaataata catcaaaagg agactatatg catcctaaaa tcggcatcat
      721 catatgcgga ttcaatgaga atcggcagtt tgtaccaaat gtttatattc aatccgtccg
      781 ttacgccaaa ggaatccctc tcctcatccc tcttgtgcga agcgacagga tgatcgatga
      841 ctatgtaagt ttatgcgacg gatttctctt ttgcggaggc gaagatatca cccctctttt
      901 attcggagaa gaaccgcaaa acggaaacgg aaaaacagac attacggtag acctttttca
      961 aatccgtctc atgaagcgcg ttctggcttc ccgaaaacct gtccttgcaa tctgcagagg
     1021 aatgcagatt ttaaatgttt cctgcggcgg aacaatctgg caggatcttt cccttatccc
     1081 acagccgacg ctcaaccaca cacagcagtc cgcatccaga ggagatgtca gccaccgcat
     1141 caaaatcgaa cgttgctcaa agctcaaaca atttgtaggc tcgtgcattt atgtgaacag
     1201 tttccaccat caatctgtca aaacacccgg gagaaacgtc tgtgtcagcg cccgttcccc
     1261 ggacggcgtc attgaggcaa tcgaactttc cggacatcct ttcgccctcg gagttcaatg
     1321 gcatcccgag tgcatgtatc gcacctcgcc tgaaatgcgt tctcttttcc atgaatttat
     1381 tgcacattca cttataaaca gcaaagattc cggacatact atttcctgaa atattattta
     1441 taaaggaggt tatattcatg acagaaatat caatccttga tcttcaaaac cttcgtcatc
     1501 ttatcggcgg atattccact acccactgta aaatgacaga ttatgcttca aaagccgaag
     1561 atccggaaat caaaaagctt tttcaggaag cggctgattc cgctatgaaa aataaacagg
     1621 aactgatgaa attcctgtaa tcttccacaa ttttcatgag acaaacaatc aaaatcatta
     1681 cagaacgagg tgaacacaat ggatgaaaag acactggttt cagatgctct tgtaggaatt
     1741 aacggtgaac tgaaaatgtt cggagatatg atcccacaga cagaaaacaa agaattaaaa
     1801 caatgcttaa aacagatccg caatcagtgt gagatggcac aggaaaaaat gtacactgcc
     1861 gcacgggaaa aaagttnnnn nnnnnntcct gccgaaaaag catctatgca ggaaatcgaa
     1921 catgtcaaat ctattttaaa cgtaaaataa gccagttcag ggttcaggcg ataaaaaact
     1981 gccgcctgaa ccctgatcca actatagaac caacttttca taagatatat ttttcaatcg
     2041 cctccgcagc tccgtcatgg cgattcgacg acgttacata atctgctcgt tgtttcaaca
     2101 tttcctctgc atttcccata gcaattttta aaccggattc ttcaaacatg aatgaatcat
     2161 tttccccgtc tccgatgcag gcaatctcct ctttctctat gccaagatga gcagaaagcg
     2221 ctttcaggcc attccccttg gacgcatctt ttgaaaatat ttcggcataa atcccggtat
     2281 acgcagcttt cacctgagga tacaccgaaa ggatctcttt taatttttcc ttttcttttg
     2341 acgtaggaaa ataaaactga agttcttcta catcacaatc actttttgaa agaatttctt
     2401 ccatgtttct cacgcagcaa accgccgctg catctttccc gtaaacaatg cggcctgccg
     2461 aagtaaaaag ctttccctga gcaaaatagc ggtgtcttac atgcgccgca atgccgaatt
     2521 tttcttttcg gcagtcggaa aggatcgaaa gcgcatcttc tttttttatc agagccctga
     2581 acaatgtctt cttttctttt acatctgtca ccatagctcc gtttgaagtg atcacataac
     2641 gaaacagatc tttgtttttt tcgtcatctt cggttttgct ctcccttagc acacctgccg
     2701 ccagcctgtg cggaatacac cctaaatttc gtcccgtaca cggaacaacc gtaatcccgg
     2761 ctttcgcagc ttttcgcagt acatccagcg ttctgtccgt catacggctt ctctgatcta
     2821 aacatgttcc gtccatatcg attgcaagta attttatcat aatctcttac ttcctttttg
     2881 cgatactacg cgcaacgtaa actccgctgg cagaagcatg ggacaaagag tgagtcacac
     2941 cgcttccatc cccgatcaca tataatcctt tgtgatttgt ctcaagatgc tcatctaact
     3001 tcacttccat attataaaac ttcacttcca caccatagag aagcgtatcg tcattcgccg
     3061 taccaggcgc aatcttatca agagcataga tcatctcaat gattccgtct aaaatacgtt
     3121 tcggaagaac aagacttaaa tctcccggct ccgctgacag tgtcgggcgc acgaatcctt
     3181 cttcaatacg ttttgccgta cttcgccttc ctcttgtcag atctccgaag cgctgcacga
     3241 tcacgcctcc gccgagcata ttggataagc gtgctatgct ttctccatat ccattgctgt
     3301 ctttaaacgg ttccgaaaaa tgtttcgcca caagaagagc aaaatttgta ttttccgtct
     3361 gcttcttcgg atcttcataa ctatgaccgt ttactgtcac aattccgttt gtattctcat
     3421 ttacaacaat accgtaggga ttcatgcaaa atgttcttac cttatcttca aacttttccg
     3481 tacgatacac aattttactt tcatacagtt catctgtcag atgggagaag atcactgccg
     3541 gaagttcaac tcttacacct agatccaccc tgtttgagtt tgtctcgata tcaagacttt
     3601 cgcacacttt ctccatccat ttgcttccgc ttcttcctac ggaaataata cactcctcac
     3661 actcgtctgt actgtccccg cttatcacac gataaccgcc gtcaaccgcc tccagttcat
     3721 ccacacgata attaaaccgg aattctactt tttctttcag ctctttatat agattttcca
     3781 atacaatata attgatatct gttcccaaat gccgcacaga agcatctaat aacttcagtt
     3841 cattttgaat acaaagtttt ttaatatccg ttcccgcagt agaatacatc tttgttccct
     3901 ctccgccgta ggcaagattg atctcatcga cgtatttcat aaggtcgatc gcagtatctt
     3961 taccgatata ttcatacagt gtaccgccga aatcatttgt aatgttatat tttccgtctg
     4021 aaaatgcccc tgcgcctcca aaaccgctca tgatcgcaca agttttacag ttaatacagc
     4081 tttttatctt ctttccgtct atcgggcatt ttcttttctc aagagaattc cccgcttcaa
     4141 atacagcgac ttttagttcc ggcgcttttt ttgcaagttc atatgccgca aagattcctc
     4201 ccgggcctgc tccaataatg atcacgtcat actgactcat atgtttctcc tttcgttacg
     4261 gtcattttac tctcactgtt tctattatag ccaac
//
LOCUS       DBMDXF010000161         4292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4887_length_4292_cov_1.942973, whole genome shotgun sequence.
ACCESSION   DBMDXF010000161 DBMDXF010000000
VERSION     DBMDXF010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_4292_cov_1.942973"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            203..346
                     /gene="scfA"
                     /locus_tag="ACZPD4_06790"
     CDS             203..346
                     /gene="scfA"
                     /locus_tag="ACZPD4_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR1378801.1"
                     /translation="MKHIKTLNTQTLNHTVKKGGCGECQTSCQSACKTSCTVGNQTCE
                     QKK"
     gene            451..1854
                     /gene="scfB"
                     /locus_tag="ACZPD4_06795"
     CDS             451..1854
                     /gene="scfB"
                     /locus_tag="ACZPD4_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1378802.1"
                     /translation="MIHLYQNNGYNIVLDVNSGAVHVVDKAAYDVIGALEEQNELHTA
                     ETLKTPETFEFLRSKLGSTYAEEDIRDILEAVATITQEGRLFTRDVYEDYIDEVKQRK
                     TVVKALCLHIAHDCNLACRYCFAEEGEYHGRRALMSYEVGKKALDFLIANSGKRRNLE
                     VDFFGGEPLMNWQVVKDLVAYGREQEKIHDKHFRFTVTTNGVLLNDEIQEFVNKEMDN
                     VVLSLDGRKEVNDKMRPFRNGKGSYDLIVPKFQKLAESRNQEKYYIRGTFTRNNLDFS
                     NDVLHFADLGFEQISIEPVVGEDTDPYAIRKEDLPKIFEEYDKLAKIMVERERSGRGF
                     TFFHFMIDLEGGPCVSKRLSGCGSGTEYLAVTPWGDLYPCHQFVGQEEFLMGNVDEGI
                     THTEIADEFRGCSVYSKESCKNCFARFYCSGGCMANSYKFNHSINDTYEVSCEMERKR
                     VECAIMIKAALADADAQ"
     gene            1956..>4292
                     /gene="secD"
                     /locus_tag="ACZPD4_06800"
     CDS             1956..>4292
                     /gene="secD"
                     /locus_tag="ACZPD4_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR1378803.1"
                     /translation="MKKSRGILSLILVAALSVLLGVTTIRGLDSEGMGAAKNINLGLD
                     LEGGVSITYQVKGDKPSQEDMDDTVYKMQRRVEQYSTEAQAYQEGDNRISIEIPGVED
                     ANAILEELGQPGSLYFIKHKDADGNENYTLGAEGYVLNKTIDELKANGSIVLTGSEVK
                     SATAGAWDDQKTGKKMYGVDLTLNAEGTDAFAAATTEAYNNGNDTIAIYYDGELISVP
                     SVNAIIENGQAQITGSASYEEADNIASTIRIGGLNLELEEISSKVVGAQLGEEAISTS
                     LKAGAIGLAAVCLFMIFVYLLPGFASSIALVIYVGLILLLLNAFDITLTLPGIAGIIL
                     GIGMAVDANVIIFARVKEELSAGASVHSALRAGFHKAMSAILDGNITTLIAAAVLWLR
                     GSGPVKGFAQTLALGIVVSMFTALVVTRLLVYSFYAVGIRNPKVYGRVKEERKPIDFL
                     GKKKVFFVVSIALCLSGFVVMGINSARDVGALNYSLDFVGGTATTVTFDKEYTLEEVD
                     SQMVPELEKITGDKNVQVQTVKGTNQVVFKTQTLDLEKREAFESYMQNEFGVKEEIAT
                     ENISSTVSSEMRMDAVIAVIIATIFMLLYIWFRFKDIRFATSAVVALLHDVLVVLAFY
                     AIARVSVGNTFIACMLTIVGYSINATIVIFDRIREEMKNSSRNTDLKDLVNRCITQTL
                     TRSIYTSLTTFVMVAVLYVMGVSSIKEFALPLMAGIVCGAYSSVCITGALWYVMKTGK
                     NAKVQAASVPAKQVESVKTESSEESSSDGNVQKAAKSKT"
BASE COUNT         1356 a          749 c         1096 g         1091 t
ORIGIN      
        1 cctttcttaa atgtttaggt ttggtagctt aaagcataac acagaaaagg tatttccgct
       61 ttttaattat tcagttgtaa cacatgtatt gtaatcctac attctcaatc ttgcagagca
      121 gttaaaaaac tctttaaaaa tggcgcatac tgtgctataa ttttatgagc gtaataatgt
      181 tataagatta ggaggacagg acatgaaaca tattaaaaca ttaaatacac agacattgaa
      241 tcacacagta aaaaaaggcg gatgcggcga gtgccagaca tcatgccagt cagcatgtaa
      301 gacatcttgt actgttggaa accagacttg cgagcagaag aaataattaa aatatcgtaa
      361 gaatacgggg caatagggtg aagaaggcag cggtctttaa aagccgctgc cttatgcttg
      421 tttgaacgaa atgaaagaga ggtatttttc gtgatacatt tgtatcagaa taacggatat
      481 aatatagtgt tggatgtcaa cagcggtgca gtgcatgtcg tagataaggc agcgtacgat
      541 gtgatcggcg cgttagaaga gcaaaatgaa ttacatacgg cagaaacatt gaaaacaccg
      601 gaaacttttg agttcttaag atcaaaactc ggaagtacat acgcagaaga agatataaga
      661 gatattttgg aagctgtcgc aacgattacg caagagggaa gactttttac gagagatgtt
      721 tatgaagact acattgatga agtgaagcag agaaaaacag ttgtcaaggc gctttgcctg
      781 cacatcgcac atgactgtaa tctggcctgc agatactgct tcgcagaaga aggggaatat
      841 catggacgcc gggcgcttat gtcttatgaa gtaggaaaaa aagcgcttga tttccttatt
      901 gcgaattcgg gaaaaagaag aaatctggaa gtagactttt tcggcggaga accgcttatg
      961 aactggcagg ttgtaaaaga tcttgtcgct tacgggcgag agcaggaaaa aatccacgat
     1021 aagcatttcc gttttacagt gacgacaaac ggagtgcttc taaatgatga gatccaggaa
     1081 tttgtcaata aagagatgga taacgtcgta cttagtttgg atggcagaaa agaggttaac
     1141 gacaaaatgc gtccgtttag aaacggaaag ggaagttatg atctcattgt gccgaaattt
     1201 cagaaacttg cagagagccg gaatcaggag aagtattaca taagaggaac atttacgaga
     1261 aataacctgg atttctccaa cgacgtactt cattttgcgg atcttggatt tgagcagatt
     1321 tcaatcgagc cggtcgtagg agaagataca gatccttatg cgatcagaaa ggaagatctt
     1381 ccgaagattt ttgaagaata tgacaagttg gcaaagatca tggtagagcg tgaaagaagc
     1441 ggacggggct ttacattttt ccattttatg atcgatttgg aaggcgggcc gtgcgtttca
     1501 aaacgtctgt cgggatgtgg atcgggaaca gaatatcttg cagtaactcc gtggggagat
     1561 ttgtatccat gtcatcaatt tgtcggacag gaagaattcc ttatgggaaa tgtggacgaa
     1621 ggtattacgc atacggagat tgcggatgaa ttcagaggat gcagcgtata ttcaaaagaa
     1681 agctgcaaaa attgtttcgc aagattttat tgcagcggcg gctgtatggc gaactcttat
     1741 aagttcaatc attcgatcaa tgatacatat gaagtaagtt gcgagatgga gagaaaacgt
     1801 gttgaatgtg cgatcatgat caaagctgca ttggccgacg ccgacgcaca gtaacggaaa
     1861 agcgcataga gcataaagaa aggtacatga gcggacggaa taaaacgtct gcataaatgt
     1921 agataaaaaa gaaaagtaag aaaggaaaag aagtcatgaa aaagagcaga ggtatattaa
     1981 gccttattct cgtggcagca ctttcagtcc tgctcggtgt gactacgatc cgcggattgg
     2041 attccgaagg aatgggcgca gcgaaaaata ttaatttggg actggatctt gagggtggag
     2101 tcagcattac atatcaggta aaaggcgaca aaccgtcaca ggaagacatg gatgacactg
     2161 tatataaaat gcagcgtcgt gtagagcagt acagtacaga ggcacaggca tatcaggaag
     2221 gtgacaaccg gatcagcatc gagatcccgg gcgtagaaga cgcgaatgca atccttgaag
     2281 aattaggaca gccgggatca ctgtatttca ttaagcacaa agatgctgat ggaaatgaga
     2341 actacacact tggagcagaa ggttatgtat tgaataaaac gatcgatgaa ctgaaagcga
     2401 acggttctat cgttttaaca ggatcggaag tgaaatccgc tacggcaggc gcatgggacg
     2461 atcagaagac aggtaagaaa atgtatgggg tagatcttac gctgaatgca gaaggaacag
     2521 atgcgtttgc ggcagctaca acagaagcat acaacaacgg aaacgatacg atcgcaatct
     2581 attatgacgg agaattgatc agtgttccga gtgtaaatgc aatcattgaa aacggacagg
     2641 cacagatcac aggctctgca tcatacgaag aagcggataa tatcgcatct acgatccgta
     2701 tcggaggtct gaatctggaa ctggaagaga tcagttcaaa agtagtcggc gcacagctcg
     2761 gtgaagaagc gatcagtaca agtcttaagg caggagcgat cggtcttgca gccgtatgtc
     2821 tgtttatgat ctttgtgtac ctgcttccgg gatttgcatc aagtatcgcg cttgtcattt
     2881 atgtcggctt gatcttactt ctgttaaatg cgtttgacat cacgcttaca cttcctggta
     2941 tcgcaggtat tatcctcggt atcggtatgg cagtagacgc gaacgttatc atttttgctc
     3001 gtgtgaaaga agaattgtca gcaggagcgt ctgttcattc agcgcttcgg gcaggatttc
     3061 ataaagcgat gtcggcaatc cttgacggaa atattacaac attgatcgcg gctgcagtat
     3121 tgtggcttag aggaagcggt ccggtaaaag gatttgcaca gacattggct cttggtatcg
     3181 tcgtttcgat gtttacggcg cttgttgtaa caagactgct cgtatattct ttctatgctg
     3241 tcggcatccg caatccgaaa gtatacggca gagtaaaaga agagagaaaa ccgatcgatt
     3301 ttctcggaaa gaaaaaagtg ttctttgtcg tttctatcgc actttgcctg agcggatttg
     3361 ttgttatggg aatcaattcc gcaagggacg tgggagcgct gaattacagc cttgattttg
     3421 tcggcggtac ggcgacaacg gtaacgtttg acaaagagta tacattagaa gaagttgaca
     3481 gtcagatggt gcctgaactt gaaaagatca caggcgataa aaacgtacag gtacagactg
     3541 taaagggaac aaatcaggta gtgtttaaaa cacagactct tgaccttgaa aagagagaag
     3601 cttttgaatc ttatatgcag aacgagtttg gcgtaaaaga agagattgcc acagaaaata
     3661 tcagttctac ggtaagttcc gaaatgagaa tggatgcggt gatcgcagtt attatcgcga
     3721 cgatcttcat gcttttgtat atctggttca gatttaaaga tattcgtttt gctacgagcg
     3781 cggtagtggc gcttctgcac gacgtgcttg tcgtacttgc attttatgcg atcgcccgtg
     3841 tttccgtggg aaatacattt atcgcatgta tgctgacaat cgtcggatac tctattaatg
     3901 cgacaatcgt tattttcgac cgtatccgtg aggagatgaa gaacagttcc agaaacacag
     3961 atctgaaaga tcttgtaaat cgctgtatca cacagacgct gacaagaagt atttatacat
     4021 cgctgacaac atttgtcatg gtggcggttc tgtatgtgat gggtgtaagt tcgatcaaag
     4081 aattcgcact tccgcttatg gcgggaatcg tttgcggagc ttattcatct gtatgtatca
     4141 caggcgcgct ctggtatgtt atgaagaccg ggaagaatgc aaaagttcag gctgcttccg
     4201 taccggcgaa acaggttgaa agtgtaaaga cagagtcgtc agaagaaagc tcatcggacg
     4261 gaaatgttca aaaagcagca aaatcaaaaa ca
//
LOCUS       DBMDXF010000162         4246 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4954_length_4246_cov_1.630639, whole genome shotgun sequence.
ACCESSION   DBMDXF010000162 DBMDXF010000000
VERSION     DBMDXF010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4954_length_4246_cov_1.630639"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="gdhA"
                     /locus_tag="ACZPD4_06805"
     CDS             complement(<1..1320)
                     /gene="gdhA"
                     /locus_tag="ACZPD4_06805"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR1378804.1"
                     /translation="MSYVDEVIDLVVKKNPAEPEFHQAVKEVLESLRVVIEANEEKFR
                     KEALLERLVEPERQIKFRVPWVDDNGQVQVNTGYRVQFNSAIGPYKGGLRLHPSVNLG
                     IIKFLGFEQIFKNSLTGLPIGGGKGGCDFDPKGKSDREVMAFCQSFMTELCKYIGADT
                     DVPAGDIGTGAREIGYMFGQYKRIRGVYEGVLTGKGLSYGGSLARKEATGYGLLYLTQ
                     EMLKINGKELAGKTVAVSGSGNVAIYATEKAQELGAKVVTVSDSTGWVYDPEGIDLAA
                     LKEIKEVNRARLTEYKKYRPNSEYHEGKGVWSVKVDIALPCATQNELHLEDAEMLVAN
                     GCMAVAEGANMPTTMEATEYLQENGVLFAPGKAANAGGVATSALEMSQNSERLSWTFE
                     EVDAKLQSIMVNICHNMADAAEKYGFKGNYVVGANIAGFEKVADAMTA"
     gene            complement(1572..1748)
                     /gene="rpsU"
                     /locus_tag="ACZPD4_06810"
     CDS             complement(1572..1748)
                     /gene="rpsU"
                     /locus_tag="ACZPD4_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1378805.1"
                     /translation="MSNVIVKENETLDSALRRFKRNCAKAGIQQEIRKREHYEKPSVR
                     RKKKSEAARKRKYN"
     gene            complement(1925..>4246)
                     /gene="alaS"
                     /locus_tag="ACZPD4_06815"
     CDS             complement(1925..>4246)
                     /gene="alaS"
                     /locus_tag="ACZPD4_06815"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1378806.1"
                     /translation="TWEFLTEVVGLDPNRLYPSVYEDDAEAFEIWNKKMGIPAERIFK
                     FGKADNFWEHGSGPCGPCSEVYYDRGEKYGCGSPDCTVGCECDRYMEIWNNVFTQFDN
                     DGNGNYSELEQKNIDTGMGLERLASVVQDVDSIFDVDTIKALRDHVCRLAEKEYGEEY
                     NNDVSIRVITDHVRSVTFMISDGIMPSNEGRGYVLRRLLRRACRHGRLLGIHKGFLPE
                     LAETVIAGSKDGYPELEEKKEFILNVISKEEEQFNKTIDQGLGILAEMISDMEKKEEK
                     ILNGEDAFRLYDTYGFPLDLTKEILEEKGLCVDEDGFKASMEVQRKKARDAREVTNYM
                     GADVTVYESIDPNVTSEFVGYEHLTWESDVTVLTTETEIADALSEGEHGTVFVQETPF
                     YATSGGQEADTGYIRTAEGEFKVEDTVKLLGGKVGHVGVMTKGMIRSGDKVVLEVDAK
                     KRALSARNHSATHLLQKALRTVLGSHVEQAGSSVNEDRLRFDFTHFSAMTEDEITQVE
                     KLVNESIAKAYDVDIKNMPIEEAKKTGAQALFGEKYGDIVRVVNMGDYSIEFCGGTHV
                     NNTSEIMALKIVSETGVAAGVRRIEALTSEGLMKYYAQTEEKLKNAAALLKATPDNLS
                     DKIHHMIAENKTLHSEVESLKSKIAQSAAGDVLDQVKEVKGIKLLAAQIEGVDMNGLR
                     ELGDQLKEKLGDGVVVLASGSDGKVSLMATATDGAMKKGAHAGNLVKAIAGCVGGGGG
                     GRPNMAQAGGKNPSGIQDALKKAEEVLAEQIKE"
BASE COUNT         1037 a         1106 c          695 g         1408 t
ORIGIN      
        1 cgctgtcatt gcatctgcaa ctttctcaaa tccggcaatg ttggctccta ctacatagtt
       61 acctttaaat ccgtattttt cagctgcatc agccatgtta tggcagatat ttaccatgat
      121 cgactgcagt ttcgcatcta cttcttcaaa tgtccagctt aagcgctcgc tgttctggga
      181 catttccaga gcagatgttg cgacaccgcc tgcatttgct gcttttcccg gtgcgaagag
      241 gactccgttc tcctgaaggt attctgttgc ttccatcgta gtcggcatat tagcgccttc
      301 tgcaactgcc atacatccgt ttgcaacaag catttctgca tcttcaagat gaagttcatt
      361 ctgtgttgcg catggaagag cgatatcaac tttcacagac catactcctt ttccttcgtg
      421 atattcggaa ttcggacgat attttttata ctctgtcagt cttgcacgat ttacttcttt
      481 aatttctttc aatgctgcaa gatcaattcc ttccggatca tatacccatc ctgtagaatc
      541 acttactgtc actacttttg ctccaagttc ctgtgctttt tccgtcgcat aaattgcaac
      601 attacctgaa cctgaaactg caactgtctt tcctgccagt tcttttccgt tgatcttaag
      661 catctcctgt gtcaggtata acagaccata tcctgtcgct tcttttcttg caagagaacc
      721 gccgtaactt aagccttttc ctgtaagaac gccttcgtaa acgccgcgga ttctcttata
      781 ttgaccgaac atatatccga tctctcttgc gcctgttccg atatctcctg ccggaacgtc
      841 tgtatctgcg ccgatatatt tacagagttc tgtcataaaa ctctgacaaa atgccataac
      901 ttctctgtcg gatttgccct ttggatcaaa atcacatccg cctttaccgc ctccgatcgg
      961 aagtcctgta agggaatttt taaatatctg ttcaaatccg aggaacttaa tgataccaag
     1021 atttactgaa ggatgaaggc gaagtccgcc tttatacggt cctattgcac tgttgaactg
     1081 tacgcggtat cctgtattaa cctgaacctg tccgttgtca tctacccacg gaacacggaa
     1141 cttaatctgt ctttcaggtt cgacgagtct ttcaagaaga gcttctttac ggaacttttc
     1201 ctcatttgct tcaattacaa cacgaagaga ttcaagaacc tcttttactg cctgatggaa
     1261 ttccggttct gccgggtttt tcttcacaac taaatcaatt acctcatcaa cgtatgacat
     1321 ctcaaattac ctcctgaatg aatttacgga tcatatccga catagaattt tttctactcg
     1381 tacattttaa aaggaaaatg aaagattgtc aacgtaaaat ttcaattttt tgcctttatt
     1441 acatataata tatcctattt tgaaagaaaa acatcttgtt ttattcattt tgggcgctat
     1501 ataatttaat cagattcttt cttccataac gaaaaacagg atgccgtcgg catcctgttt
     1561 aagtgcactt attaattata tttacgtttt ctagcagctt cagatttttt cttacgtctt
     1621 acgcttggtt tttcatagtg ctcccttttg cggatctcct gctgaatacc agctttagca
     1681 cagtttcttt tgaatctgcg taaagcgcta tctaacgtct cgttttcttt aacgattaca
     1741 tttgacatag tctcacacct aacctcccct ccagccttgt attgtgcata atacgactgt
     1801 tcgggtattt ttattgcact aataaagatt ataacatatt tttttctccc gtcaactatt
     1861 tccgagcaaa aacttcataa aagcttataa aaaatgcatt tttctatatt tttacaaaat
     1921 aaaactattc tttgatctgt tctgcaagaa cttcttccgc ttttttcaat gcatcctgga
     1981 ttcctgacgg attctttcct cccgcctgcg ccatattcgg acgaccaccg ccgccgcctc
     2041 cgacacatcc tgcgatcgct ttcacaagat ttccggcgtg agcgcctttt ttcatcgctc
     2101 cgtctgtcgc agtagccatc agactgactt taccatcgct tcctgatgca aggacgacaa
     2161 ctccgtctcc taatttttct ttcaactgat cgccaagttc tctaagtccg ttcatgtcaa
     2221 cgccctcgat ctgcgctgcc aaaagtttta ttccttttac ttctttcacc tgatccagta
     2281 cgtctcctgc cgcactttga gcgatcttac ttttcagact ttccacttcg ctgtgtaatg
     2341 ttttgttttc agcaatcata tgatgaatct tatctgataa attgtcaggc gttgctttta
     2401 aaagcgctgc cgcatttttc aatttttctt ctgtctgcgc ataatatttc atcagccctt
     2461 ctgaagtcaa agcctcaatt ctgcgaactc ctgccgccac acctgtctct gaaacaattt
     2521 tcagagccat aatctccgat gtgttattca catgagttcc gccgcaaaat tcaatagaat
     2581 aatctcccat attcacgaca cggactatat caccgtattt ttctccgaac agagcctgcg
     2641 ctcctgtctt ttttgcctct tcgatcggca tatttttaat atctacatcg tatgcttttg
     2701 caatactttc attgacaagt ttctcaacct gtgtgatctc atcttctgtc atcgccgaaa
     2761 aatgtgtaaa atcaaagcga agtctgtctt catttacgct tgatcctgcc tgttctacat
     2821 gacttcccaa cactgtacga agtgcttttt gaagaagatg agttgcgcta tgattccttg
     2881 ctgaaagagc acgctttttc gcatccactt ccagcactac tttatctccg gatctgatca
     2941 ttccctttgt cataactccg acatgaccga cttttccgcc gagcaatttc actgtatctt
     3001 ctactttaaa ttcaccttct gcagtacgga tatatcctgt atccgcctcc tgacctccgc
     3061 ttgtcgcata aaacggggtc tcctgaacaa agaccgtacc gtgctctcct tcagataatg
     3121 cgtccgcaat ctccgtttcc gttgtaagaa cagtcacgtc tgattcccat gtaagatgtt
     3181 catatccgac aaattcactt gtcacattcg gatcgataga ctcatatact gttacatcag
     3241 ctcccatata attcgtcact tcacgggcgt cacgggcttt ttttctctga acttccatgg
     3301 aggctttaaa tccatcttca tccacacaga gtcctttttc ttccaaaatt tcttttgtca
     3361 gatcgagagg gaatccatat gtgtcataca ggcggaaagc atcttcgccg tttaatatct
     3421 tttcttcttt tttctccata tcagagatca tctctgccaa aattccaagt ccctgatcta
     3481 ttgttttatt gaactgctct tcctctttgg aaatcacgtt caaaataaat tctttcttct
     3541 cctccagctc cggatacccg tcttttgatc ccgcaatgac agtttctgca agctccggca
     3601 ggaatccttt gtgaatgccg agaagtcttc catgacggca ggctcttcta agcagacggc
     3661 gaagaacata accgcggcct tcattagacg gcataattcc atctgagatc ataaaagtca
     3721 cagaacggac atgatctgta atgacgcgga tcgagacatc attgttgtac tcttccccgt
     3781 attctttttc cgcaaggcgg catacgtgat cacgaagcgc tttaatcgta tcaacatcaa
     3841 aaatagaatc tacatcctgc acaacagacg ccagacgttc caatcccatt cccgtatcaa
     3901 tattcttctg ctccagctca ctgtaattgc cgtttccgtc attgtcaaac tgtgtaaata
     3961 cattattcca aatttccatg taacggtcgc actcgcagcc tactgtacaa tcaggacttc
     4021 cacagccgta tttttctccc ctgtcataat acacttctga acagggtccg cacggaccgg
     4081 aaccatgctc ccagaaatta tctgcttttc cgaatttaaa gatacgttct gccggaatcc
     4141 ccatcttctt attccatatt tcaaatgctt ccgcatcgtc ctcatatacg gacgggtaca
     4201 gtctattcgg gtcaaggccg actacttctg tcagaaattc ccatgt
//
LOCUS       DBMDXF010000163         4246 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4955_length_4246_cov_1.479373, whole genome shotgun sequence.
ACCESSION   DBMDXF010000163 DBMDXF010000000
VERSION     DBMDXF010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_4246_cov_1.479373"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(391..1257)
                     /locus_tag="ACZPD4_06820"
     CDS             complement(391..1257)
                     /locus_tag="ACZPD4_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1378807.1"
                     /translation="MSIIQIIKEQMNMTERERDICRYILENPEKIEEMSSRELGHATF
                     TSAASVTRFCQKIGVKGFPEFKMQFVRELRTGGMEDVSAVTMSERENVVTMVRKAEKI
                     QRQAVEETNKELSYTQLVRIGKLIADASCVDFYVYDMNIYLAKYGCALFFHAGKLSSV
                     HSEINIQGLHAAMPADGHVAIIISHTGKNEQLAEIEKMLHRGGTKIIVISGNRKGVVG
                     QYATEFLYAAGSEKVEEFWSSTFFASGKYLLDILYGMEFSRRYEENIALNSRYEKSGK
                     NLLWRFSEKESD"
     gene            1431..2021
                     /locus_tag="ACZPD4_06825"
     CDS             1431..2021
                     /locus_tag="ACZPD4_06825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1378808.1"
                     /translation="MKYKNIVFDFGNVIGKFNGRDLVKHFSSCEEDQELLFTAIFSRW
                     DDIDNGSVDYEQNIDNIAASLPDRLESAVRTLFREWPRHLTPINDTLRFIDELKERNI
                     PLYLLSNAPTYFAEYAVNYDFLKEFSGIVFSAPLKMAKPDPAIYRYLFETFSLDPKEC
                     FFIDDLEKNINAARSLGMDGIVFNGNIDDVKKAIGF"
     gene            complement(2111..2611)
                     /gene="lspA"
                     /locus_tag="ACZPD4_06830"
     CDS             complement(2111..2611)
                     /gene="lspA"
                     /locus_tag="ACZPD4_06830"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1378809.1"
                     /translation="MKIRWFPITAALAAADQAAKCYAENKLAKGEERAITDKVVLRHV
                     QNEGVCMSLLEDEPETARILAGAASGAVLGWHAFTVVTKKKRFWKKAGLSLMAAGSIS
                     NMFDRLVRGHVVDYVGFRLEDKHLEGITYNFADFFIAAGAVITVMTKLFRPGSKKKKK
                     AKTKSV"
     gene            complement(2601..3314)
                     /locus_tag="ACZPD4_06835"
     CDS             complement(2601..3314)
                     /locus_tag="ACZPD4_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1378810.1"
                     /translation="MKDFFENFGKRFGETAEMVTNKAGEMIEIQRVKSQIRDLARENA
                     VDMMELGKAVYERYKSGEELDDSTAQICDSIKEREKEIEKYERKIAVLKGSAACVKCG
                     RMVSEDMAYCPYCGEKMEARDMEEATEEYAEKVKEKAADVAEAMADKAEETAKKIGEA
                     AQKAAYKTEEVLEKAADKTTQAVHKAAEKTTEAVHKAADKAEETAHKSADKAEEAAHR
                     SAEKAEDAADKTVEKLNED"
     gene            3572..>4246
                     /locus_tag="ACZPD4_06840"
     CDS             3572..>4246
                     /locus_tag="ACZPD4_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR1378811.1"
                     /translation="MLDRETCREHILSEDYRDFIGNHVRTPFFNSLMNVHRCEQEAGF
                     SYKCFYLPKEISDPITLPKYSYASIPNCYSPLNMQTLNEAGILPVQNYPTLQLKGRNI
                     LIGFLDSGIDYTNPVFQNLDNTTRIRAIWDQTVQTGTMPEHFSYGSEFTEEQINEALR
                     SDSPFDLVPSTDETGHGTYAAALACGSAVPEEEFLGAAPEASIAVVKLKQAKQYLRDY
                     YFIPSDA"
BASE COUNT         1082 a         1056 c          688 g         1420 t
ORIGIN      
        1 caggactttc ttatggactt acaatcgatg ttcgatcact tagttgatat cactgaaccg
       61 atctgccaac gtattgattc taaccttgct tccatgagta tgatgatcaa acatcatatt
      121 gctccgattc taagagagtt tgtgcacatt tctcttttga tcttgacatc agtttaaata
      181 atctgatgat ttttttcaat tcattcatac ttttttgttt atctgtttca aaattaacta
      241 ctttcgcgtt ccgttttatg caatttcgtt tcagatgtaa aaaatctgtc ctgtattttg
      301 tgcaaaatgt ataaagcata aaaacttctc catatctgat ctttcagaaa ggaaaagttt
      361 tcactctgtc tttttactac tctgttattt ttaatcactc tctttttcgg aaaatctcca
      421 cagaagattc ttgcctgact tttcatatct gctgtttaaa gcaatatttt cttcgtaccg
      481 tctgctaaat tccattccat ataaaatatc aagaagatat ttccccgatg caaaaaacgt
      541 ggaactccaa aattcttcca ctttttcaga acctgccgcg tataaaaact ctgttgcata
      601 ttgacctacc acgcctttcc tgtttccgga gattacgata attttcgttc ctcccctgtg
      661 cagcattttt tcaatctccg ccagctgctc gtttttgccc gtatgactga taataattgc
      721 cacatgtccg tctgcgggca tggccgcatg cagcccttgt atattaattt ccgagtgaac
      781 actcgacaat ttcccggcat gaaaaaacaa ggcgcatccg tatttcgcca aataaatatt
      841 catatcatat acataaaaat caacacaaga cgcatctgcg atcaactttc cgatccgcac
      901 taactgagta taagagagtt ctttattcgt ctcctccacc gcctgtcttt gtatcttttc
      961 cgctttccga accatagtaa ccacattttc ccgctccgac atcgtaactg cggaaacgtc
     1021 ttccattccc ccggttctca actcccggac aaactgcatt ttaaattcag gaaaaccttt
     1081 tacccctatc ttttgacaaa atcgagttac ggaagcggcg cttgtaaaag tcgcatgccc
     1141 taattctctt gaagacattt cttctatttt ttccggattt tccaaaatat atctgcatat
     1201 atcccgctct cgttccgtca tattcatctg ttcttttata atttgaataa tagacataca
     1261 acacctcttt cttgtttttt ccagtataac acgcaccatc tgtctactgc aataaatgta
     1321 gattttaaac atgtatgatc taaattcctt atcttttaaa gcatttgaca aagtatgaaa
     1381 atgccactaa actaatgatg ataattgtaa taatatgagg tataaatgct atgaaatata
     1441 aaaatattgt ttttgatttc ggaaatgtaa taggtaaatt taacggacgt gatcttgtaa
     1501 aacatttttc ttcctgcgaa gaagatcagg aacttctctt taccgcaatt ttttcaagat
     1561 gggatgatat tgacaatggt tcggtagact atgaacaaaa catagacaat attgcagctt
     1621 cactccccga ccggttagaa tctgccgtac ggacactttt tcgcgagtgg ccgcgtcacc
     1681 tgacgccgat caacgatacg ctgcgcttta tagatgagtt aaaagagcgt aacattccgt
     1741 tgtatctatt atcaaatgca ccgacctatt ttgccgaata tgccgtcaac tatgattttc
     1801 tgaaggagtt cagcggaatt gtattttccg ctcctcttaa aatggcaaaa cccgatcctg
     1861 caatctaccg ttacttgttt gaaacgtttt cccttgatcc gaaagaatgt ttctttatcg
     1921 acgatctgga aaaaaatatc aatgccgcgc gttctcttgg tatggacgga attgttttca
     1981 acggcaatat cgacgatgtg aaaaaagcga ttggtttctg atctcaagaa accagtcgct
     2041 ttttagctgt accttcttct gttctgtttt tttgtttcgt tttcttttcg gattttacgt
     2101 ttttcagatt tcagacactt ttcgtttttg cttttttctt ctttttcgag cccggtcgaa
     2161 acagttttgt catcactgta atgacagctc ctgctgcgat aaagaaatcc gcgaaattat
     2221 atgtgatccc ttccaaatgt ttatcttcca agcgaaatcc cacataatct acaacgtgtc
     2281 cgcgaacgag ccgatcgaac atattactga tcgagcctgc cgccatcaat gaaagtcctg
     2341 cctttttcca aaacctcttc ttctttgtca caaccgtaaa ggcatgccat ccgagcacag
     2401 ctcccgacgc cgctcccgcc aaaatacggg cagtctcagg ttcatcttcc agaaggctca
     2461 tacagactcc ttcgttttga acgtgccgta aaacaacctt gtctgtaatc gcacgttctt
     2521 ctccttttgc aagtttattc tcggcatagc attttgccgc ctgatctgcg gcagccaaag
     2581 ctgccgtgat cggaaaccat ctaatcttca ttgagtttct ccactgtctt atctgccgca
     2641 tcttctgctt tctccgctga tctatgcgcc gcttcttctg ctttatccgc tgatttatgc
     2701 gctgtttcct cagctttatc cgccgcttta tgaactgctt ccgtcgtttt ttccgctgct
     2761 ttatgaacag cctgggtcgt cttatctgcc gctttttcaa gcacttcttc tgtcttatac
     2821 gctgctttct gcgcagcttc tcctatcttt tttgccgttt cttctgcttt atcagccatc
     2881 gcttcggcaa catccgctgc tttttccttt actttctccg catactcctc cgtcgcctct
     2941 tccatatccc gggcctccat cttctctccg cagtacgggc agtatgccat atcttccgaa
     3001 accattctgc cgcatttaac acaagctgca gaacctttca aaaccgcgat cttgcgttcg
     3061 tacttttcaa tttccttttc ccgttctttt atcgaatcac aaatctgcgc agtgctatcg
     3121 tcaagttctt cgcctgattt ataacgctca tacaccgctt tgcccaactc catcatatca
     3181 acggcgtttt cacgagcaag atctctgatc tgacttttaa ctctctggat ctctatcatc
     3241 tctcctgctt tattcgtcac catttcggca gtctctccga accgttttcc gaaattttca
     3301 aaaaaatctt tcatgtgtat caatctcctt tcgtctttta tccaaacgga cgtccgaagt
     3361 acaaaaaaca tcggtcgtat ctgatattta agttatcatt attataccct tttctgaaac
     3421 aacttcaaca tttctcattg gaatttatac ttttttcact tctgattctt cggaagcgtt
     3481 ctttagatca aacactcttt tttcattttt tcaagaatga ggttctgatt ttccgcatat
     3541 gatataaaaa tcaaatcgga gaatttattt tatgcttgac cgcgaaacat gccgtgaaca
     3601 tattttatcc gaagattacc gcgactttat cggaaaccat gtccgaactc cctttttcaa
     3661 cagtctcatg aacgtgcatc gctgtgaaca ggaagcggga ttttcttaca aatgcttcta
     3721 tcttccaaag gagatttccg acccgatcac tctgccgaaa tattcatatg cttcgattcc
     3781 gaactgctat tcgcctctta atatgcagac actcaacgaa gccggaatcc tccctgttca
     3841 aaactaccct acgcttcagc ttaaaggacg caatattttg atcggatttc ttgacagcgg
     3901 aatcgactat acaaatccgg tttttcaaaa cttagataat acaacccgca tccgcgctat
     3961 ttgggatcaa actgtgcaga ccggaacaat gccggaacat ttttcttacg gaagcgaatt
     4021 tacggaagaa cagatcaacg aagctcttag atcggattct ccgtttgatc ttgttccatc
     4081 cactgatgaa acagggcacg gaacttacgc cgctgccctt gcatgcggaa gcgccgttcc
     4141 tgaagaagaa tttctcggtg ccgcacctga agcctctatc gccgttgtga aactaaaaca
     4201 ggcaaagcaa tatctccgag actattattt cattccttct gatgcc
//
LOCUS       DBMDXF010000164         4223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4979_length_4223_cov_1.870936, whole genome shotgun sequence.
ACCESSION   DBMDXF010000164 DBMDXF010000000
VERSION     DBMDXF010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_4223_cov_1.870936"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="ACZPD4_06845"
     CDS             <1..736
                     /locus_tag="ACZPD4_06845"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1378812.1"
                     /translation="QSNLASTIAMAFDECPSSVADREYMVHSVARTTRWLKRCKAEMN
                     RLNSLEDTINHNQMLFGINQGGIYEDIRVEHARQIAELDLDGYAVGGLAVGESHEDMY
                     RILEAVVPHLPENKPTYLMGVGTPANILEAVDRGADFFDCVYPSRNGRHGHVYTNHGK
                     MNLFNAKYELDDRPIEEGCGCPACRSYSRAYIRHLLKAKEMLGMRLCVLHNLYFYNTM
                     MEEIREAIEAGEYKAYKKRKLEGMMQ"
     gene            867..1145
                     /gene="yajC"
                     /locus_tag="ACZPD4_06850"
     CDS             867..1145
                     /gene="yajC"
                     /locus_tag="ACZPD4_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1378813.1"
                     /translation="MGEMVTMLVLWLVVIGVMWFLLMRPQKKEQKRIQQMLASMEVGD
                     TALTTSGFYGVIIDITDEDVIVEFGNNKNCRIPMQKAAIAQIEKPNAD"
     gene            1355..2437
                     /gene="leuB"
                     /locus_tag="ACZPD4_06855"
     CDS             1355..2437
                     /gene="leuB"
                     /locus_tag="ACZPD4_06855"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1378814.1"
                     /translation="MDMNIGVIKGDGIGPEIVDEAMKVLDKTAEVYGHQIEYTQLLMG
                     GASIDVNGVPLTEETLEQAKKSDAVLMGSIGGDAKTSPWYRLEPSKRPEAGLLQLRKG
                     LNLFANLRPAVLYSELKGACPLKEEIAEQGFDMMIMRELTGGLYFGKRSTEEEDGVLV
                     ARDELTYSETEIRRIAKRGFDIAMKRRKKVTSVDKANVLDSSRLWRKIVEEVAKEYPE
                     VTLEHMLVDNAAMQLVKDPAQFDVILTENMFGDILSDEASMVTGSIGMLASASLNDTK
                     FGLYEPSGGSAPDIAGKGIANPIATILSAAMMLRYSFDLDQEADAIEAAVEKVLEEGY
                     RTIDIMSEGMTQIGTKEMGERICSYI"
     gene            2462..4135
                     /gene="ilvD"
                     /locus_tag="ACZPD4_06860"
     CDS             2462..4135
                     /gene="ilvD"
                     /locus_tag="ACZPD4_06860"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1378815.1"
                     /translation="MRSDTVKKGVQQAPHRSLFNALGMTQEELDRPLIGVVSSYNEIV
                     PGHMNLDKITEAVKMGVAMAGGTPIMVPAIAVCDGIAMGHIGMKYSLVTRDLIADSTE
                     ALAMAHQFDGLVMIPNCDKNVPGLLMAAARVNIPTIFVSGGPMLAGHVKGQKTSLSSM
                     FEAVGSYAAGKMNDEEIYEFENKACPTCGSCSGMYTANSMNCLTEALGMGLRGNGTIP
                     AVYSARLQLAKHAGMQIMELVRNNIRPRDIMTEDAILNALTVDMALGCSTNSMLHLPA
                     IAHEIGMDFEIDFANGISEKTPNLCHLAPAGHTYIEDLNEAGGVYAVMNELNKKGLLH
                     TDCLTVTGKTVGENIAGCENKNPDVIRPIDHPYSETGGLAVLKGNLAPDGSVVKRSAV
                     CDEMLVHEGPARIFESDEEATEAIKTGKINPGDVIVIRYEGPKGGPGMREMLNPTSAI
                     AGYGLGSEVALITDGRFSGASRGASIGHVSPEAAVGGPIALVEEGDIIQIDIPNLSLN
                     LAVSEEELKKRREKWQPREPKVKTGYLARYASMVTSGNRGAILEIPKEK"
BASE COUNT         1313 a          699 c         1152 g         1059 t
ORIGIN      
        1 tcagtctaat cttgcgtcta cgatcgcgat ggcgtttgac gaatgtccct caagcgtagc
       61 ggacagagag tatatggtcc attcggtggc gagaacgaca agatggctga aacgatgtaa
      121 agcggaaatg aacaggctga attctttgga agatacgatt aatcataacc agatgctttt
      181 tggtattaat caaggcggaa tctatgaaga tatccgcgtg gagcacgcac gacagatcgc
      241 ggagcttgat ttggacggtt atgcggtagg cggtcttgca gtaggagaga gtcatgaaga
      301 tatgtaccgt attctggagg cggttgttcc tcatcttcct gaaaataagc cgacctattt
      361 gatgggagtg ggaactccgg cgaatatttt ggaggcggta gacagaggag cggacttttt
      421 tgactgcgta tacccgagca gaaacgggcg gcacgggcat gtttatacaa atcatggaaa
      481 aatgaactta ttcaatgcaa agtatgagtt agatgaccgt ccaatcgaag aaggatgcgg
      541 atgtccggca tgcagaagtt atagcagagc atatatccgt cacctgttaa aagcaaaaga
      601 aatgctcggg atgcgtcttt gcgttcttca taacctgtat ttttataata cgatgatgga
      661 agaaatcaga gaagcgattg aagcgggaga gtataaggct tataaaaaga gaaaactgga
      721 aggtatgatg caataaccgg ataagcttct caaaattatg tttatttcaa gttacttcag
      781 tattttgaaa aatatccttg aagtatggaa gaaagttgtg tatagtagtc ttattgcgtt
      841 aggaaagtta ggaggaaatt ataatcatgg gtgaaatggt aacaatgctt gttctttggc
      901 ttgtagttat cggtgtaatg tggtttttgc ttatgcgtcc acagaaaaaa gaacagaaac
      961 gtatccagca gatgctggct tctatggagg tcggagacac agctctgacg acaagcggtt
     1021 tttacggagt catcattgat attacagatg aagatgtgat tgtagagttc ggaaataata
     1081 aaaactgtcg tattccgatg cagaaagctg cgattgctca gattgaaaaa ccgaatgcag
     1141 attgatttta agtcaattat tttgcattat gagatcaggg agaattttga aatattttca
     1201 aaattctccc tgattttttc aaattcatca ctttagcgcg tgaatttgaa aaaatcacag
     1261 agtacactct aatttttaga cgcaaatact tgaaacgtgt ataaaaatgc tgtattataa
     1321 acagtaacat tttagtttgg agggacggga aattatggat atgaacatcg gtgtgatcaa
     1381 aggcgacgga atcggtccgg agattgtaga tgaggcgatg aaagtgctcg ataaaacggc
     1441 agaagtttac gggcatcaga tagaatatac tcagcttttg atgggaggag catccattga
     1501 tgtaaacgga gtgcctctta cggaagaaac attggaacag gcgaaaaaaa gtgatgccgt
     1561 attgatggga tcgataggag gagatgcaaa gacatcgcca tggtatcgtc tggaaccgtc
     1621 taaacgtccg gaggcggggc ttttacagct tagaaagggg ttaaaccttt ttgcaaatct
     1681 gcgcccggca gttctttaca gtgaattaaa aggagcctgc ccgctcaaag aggagattgc
     1741 agaacaaggt tttgatatga tgatcatgcg ggagctgaca ggcggactct atttcggaaa
     1801 aagaagtacg gaagaagaag acggagttct cgttgcaaga gatgagctta catatagtga
     1861 aacggagata aggcgaattg cgaagcgcgg atttgacatt gcgatgaaac gtcggaaaaa
     1921 agtgacaagc gttgataagg cgaatgtatt ggattcttca agattatgga gaaaaattgt
     1981 tgaagaagtt gcgaaagaat atccggaagt aacgcttgaa catatgttag tggataatgc
     2041 ggctatgcag cttgtgaagg atccggcaca atttgatgta atattaacgg aaaatatgtt
     2101 tggtgacatt ttatctgatg aggcgagtat ggtgaccggc tcaatcggaa tgcttgcaag
     2161 cgcaagttta aacgatacaa agttcggcct ttacgaaccg agcggaggtt cggctcccga
     2221 cattgcggga aaaggaatcg caaatccgat tgctacaatt ttatcggcgg cgatgatgct
     2281 tagatattcg tttgatctcg atcaagaagc agatgcgatt gaggcggcgg tagagaaagt
     2341 actcgaggaa ggataccgga cgattgatat tatgtcagaa ggaatgacgc agatcggaac
     2401 aaaagagatg ggagaacgaa tctgttctta tatataaaac gtgaaagaga ggcataggat
     2461 aatgagaagc gatacagtga aaaaaggggt gcagcaggcg ccgcaccgtt ctttatttaa
     2521 tgcgcttgga atgacgcagg aggagttaga cagaccgctg atcggagttg taagctctta
     2581 taatgagatt gttcccgggc atatgaatct tgataaaatt acagaagcgg taaaaatggg
     2641 agttgcaatg gcaggaggaa caccgattat ggttccggct attgctgttt gcgacggtat
     2701 tgcgatggga catatcggca tgaaatattc tcttgttacg agagatctta tcgcagattc
     2761 aacagaggcg cttgcaatgg cacatcagtt tgacggtctt gttatgattc cgaactgtga
     2821 taaaaatgtg ccgggacttc ttatggctgc tgcgcgtgtc aatataccta cgatctttgt
     2881 gagcggagga ccgatgcttg caggacatgt aaaagggcaa aagacaagtt tgtcaagcat
     2941 gtttgaagcg gtcggatctt atgcggcagg caaaatgaac gatgaagaaa tttatgaatt
     3001 tgaaaataaa gcatgtccga cgtgcggttc gtgttccgga atgtatacag ccaatagcat
     3061 gaattgtctg acagaagcat tgggaatggg acttcgtgga aacggaacga ttcccgcggt
     3121 atattctgcg agacttcagc ttgcaaaaca tgcgggaatg cagattatgg agcttgtgag
     3181 aaacaatatc cgacctagag atattatgac ggaagatgcg attttgaacg ctcttacagt
     3241 cgatatggct cttggatgtt cgacaaacag tatgcttcat cttccggcaa tcgcacatga
     3301 gataggaatg gattttgaga ttgattttgc gaacggaatc agtgagaaga cgccgaacct
     3361 ttgtcatctt gctccggcag gccatacata tatcgaagac cttaatgagg caggcggagt
     3421 ctatgcggtt atgaatgagc tgaataaaaa aggacttctt catacagatt gtctgacagt
     3481 aaccggcaag acagttgggg agaacattgc gggatgcgaa aataaaaatc ccgatgtaat
     3541 tcgtccgatc gatcatccat acagtgaaac aggtggattg gctgtgttaa aaggaaatct
     3601 tgcacctgac ggaagcgttg taaaacgatc tgctgtctgc gatgagatgc ttgtacatga
     3661 aggtcctgcc cgtatttttg aaagtgatga agaggcaacg gaagcaatca aaacaggaaa
     3721 gatcaatccg ggggatgtga tcgtgatccg ttatgaagga ccgaaaggcg gtccgggaat
     3781 gcgggagatg ctcaatccga cttcggcgat tgcaggatac ggactcggct cggaagtagc
     3841 gcttattaca gacggacgtt tcagcggcgc gtcaagaggc gcatctatcg gtcatgtttc
     3901 tccggaagcg gcggtgggag gaccgatcgc tcttgtagaa gagggagaca ttatacagat
     3961 tgatattccg aatttatctt tgaatctggc agtttcagaa gaagaactga agaagagaag
     4021 agaaaaatgg cagccgagag aaccgaaagt aaagaccgga tatctggcaa gatatgcgtc
     4081 tatggtcaca tccggaaaca gaggtgcgat tctggaaata ccgaaagaaa aataagagcg
     4141 cggttacagg ctgtgcggat aagagataca ggatgtttaa aggagtaatt atggcaaaag
     4201 agcagaaaaa aatggtggac gac
//
LOCUS       DBMDXF010000165         4221 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_4984_length_4221_cov_1.705583, whole genome shotgun sequence.
ACCESSION   DBMDXF010000165 DBMDXF010000000
VERSION     DBMDXF010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4984_length_4221_cov_1.705583"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            324..>4221
                     /locus_tag="ACZPD4_06865"
     CDS             324..>4221
                     /locus_tag="ACZPD4_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR1378816.1"
                     /translation="MNLNKLHTLGIDIGSTTVKIAILDSENEVLFSDYERHFANIQET
                     LSDLLGRALYKLGSIQVSPVITGSGGLTLAKHLGVPFVQEVIAVSTALQDYAPQTDVA
                     IELGGEDAKIIYFEGGNVEQRMNGVCAGGTGSFIDQMASLLQTDASGLNEYAKNYKAL
                     YSIAARCGVFAKSDIQPLINEGASKEDLAASIFQAVVNQTISGLACGKPIRGHVAFLG
                     GPLHFLSELRETFIRTLKLDDEHIIAPHHSHLFAAIGSALNSKKDTPTALCELQKRLE
                     NKIQLDFEVERLDPLFETSADYDKFISRHSKHQVPIKDLATYTGKAFLGIDAGSTTTK
                     AALVGEDGTLLYSFYHNNDGDPLGTTISAIKDIYRQLPEDVEIVHSCSTGYGEALIKS
                     ALMLDEGEVETVAHYYAAAFFEPDVDCILDIGGQDMKCIKIKNQTVDSVQLNEACSSG
                     CGSFIETFAKSLNYTVEDFAHEALYAQNPIDLGTRCTVFMNSKVKQAQKEGASVADIS
                     AGLAYSVIKNALFKVIKVSDASELGKHIVVQGGTFYNNAVLRSFEKIADCEAIRPDIA
                     GIMGAFGAALIARERYGECKGTTMLSIEDIRSLEYSTTMTKCRGCTNTCRLTINHFSG
                     GRKFITGNRCERGLGKEKNTNTLPNLFDYKFHRYFDYEPLSEEDATRGIIGIPRVLNM
                     YENYPFWFTFFTKLGFRVVLSPASTRKIYELGIESIPSESECYPAKLAHGHIQWLINN
                     GIEHIFYPSVPYERNEFEDSNNHYNCPIVTSYPENIKNNIDPIIENEVDFIHPFLSFK
                     NEETIAYRLFEELGSKFSLSKNEIYSAVHDAWTELASCREDMRRKGEETIRFLNETGN
                     RGIVLAGRPYHIDPEVNHGLPEMILSYNIAVLTEDSVSHLHPVERPLNVMDQWMYHSR
                     LYAAANYVKTTENLDLIQLNSFGCGLDAVTTDQVADILTNSGKIYTTLKIDEVNNLGA
                     ARIRVRSLLAAIRVREQKKTERTIRSAAIEKVPFTKEMKATHTILCPQMSPIHFEMLE
                     AAFRGSGYRFEILPNDNKQAVDVGLKYVNNDACYPSLIVVGQIMEALLSGKYDLDKVA
                     VIITQTGGGCRASNYIGFIRRALKKAGFGHVPVISLNLSGLEGNPGFKLTPSLIMRIV
                     YGALFGDIFMKCVYHMRPYEAVPGTTDQIHRKWAKICKDFVSKGYPSRHKFKKLCRDI
                     IHDFDSIEILDIKKPRVGIVGEILVKFLPAANNHLVELLEAEGAEAVVPDLADFLQYC
                     FYNQNFKVSHLGMKKSKALAGRLGVQAVEWFRSPATK"
BASE COUNT         1269 a          940 c          835 g         1177 t
ORIGIN      
        1 accagctcaa caatcattac ctcacctttt aacattttta tctaattgta actttcacat
       61 atctgtttgt caaacatatg ttgtttcttt tcacacgttt cacacaatct ttatgtaaaa
      121 tagctatata aatcgacatt attagcgaaa acccgcataa taatcacgat tgtatttcca
      181 aaaatgatgt cgtataatgg aaaacgtcgt ataatagtat tatacattta ataggtagga
      241 actcttttct ttttcagagt tctcttttca ttatagtaat atttcgaaaa atcattattt
      301 ttttaaagtt tggagataat gcaatgaact taaacaaatt acatactctc ggtattgata
      361 tcggttctac cacagtcaaa attgcaattt tagacagtga aaacgaagtc cttttttcag
      421 actacgaacg tcattttgca aacattcagg aaacattatc cgacttgctc ggacgggcac
      481 tttacaaact cggttcaatt caggtttctc ctgtcattac aggaagcgga ggtcttactc
      541 tcgcaaaaca tctgggagtt ccttttgtac aggaggtcat tgccgtatca acagctctgc
      601 aagactatgc accgcagact gatgtggcga ttgagctcgg aggtgaggat gcgaaaatca
      661 tctattttga aggtggaaat gtagaacagc gtatgaacgg agtctgtgca gggggcacag
      721 gttcttttat tgaccaaatg gcttcccttc ttcagacaga cgcttcggga ttgaacgaat
      781 atgcaaagaa ctataaagcg ctatactcca tcgcggctcg ctgcggtgta tttgccaaat
      841 ccgacattca gcctctcatc aatgaaggag cgtccaaaga agatcttgcc gcttctattt
      901 ttcaggctgt cgtaaaccag acaatcagcg gacttgcatg tggaaaacca attcgcggac
      961 atgttgcatt tctcggcgga cctcttcact ttttatcaga actacgcgaa acctttatcc
     1021 gtactttaaa attagatgac gaacacatta tcgctccgca ccactcacac ttgttcgccg
     1081 cgatcggatc tgctttaaat tcaaaaaagg atacgccgac agctctttgc gagcttcaga
     1141 aacgccttga aaataaaatt caattagact ttgaagtaga gcgtcttgat ccattatttg
     1201 aaacatctgc agactatgat aaattcattt cccgtcatag caaacaccaa gttccgatca
     1261 aagatctggc aacttataca ggcaaagctt ttttaggaat tgatgcggga tctacaacaa
     1321 caaaagctgc tcttgtcgga gaagacggaa cactccttta ctctttttac cacaataatg
     1381 acggagatcc tctcggaaca acaatttccg cgatcaagga tatttaccgc cagcttcccg
     1441 aagacgttga aatcgtccac tcctgctcta ccggatacgg agaagcgctg atcaagtctg
     1501 cccttatgct agatgaaggc gaagtggaaa ccgttgccca ttattatgca gccgcgtttt
     1561 ttgaacctga tgtagactgt atcctcgata tcggcggtca ggacatgaaa tgcatcaaaa
     1621 tcaaaaatca aacagttgat agtgtgcagc ttaacgaagc atgttcttcc ggatgcggtt
     1681 ctttcattga aacattcgca aagtctttaa attacacggt agaagatttt gcccatgaag
     1741 ctctttatgc tcaaaatccg attgatttag ggacacgctg tactgttttt atgaactcta
     1801 aagtaaagca agctcaaaaa gaaggcgctt ccgtagcgga catctctgcc ggacttgctt
     1861 actccgtcat aaaaaacgca cttttcaaag tcatcaaagt atcagacgct tccgaactcg
     1921 ggaaacatat tgttgttcaa ggcggaacat tttacaacaa tgccgtcctt agaagctttg
     1981 aaaaaatcgc agactgcgaa gcaatccgtc ccgatatcgc cggaatcatg ggcgcattcg
     2041 gcgctgcatt gattgccaga gaacgttacg gagaatgtaa aggaacaact atgctttcta
     2101 ttgaagacat ccgttccctt gaatattcaa ctactatgac aaaatgccgc ggatgtacaa
     2161 acacttgtcg tcttacgatc aatcacttca gcggcggccg aaaatttata accggaaacc
     2221 gatgtgaacg aggtctcgga aaagaaaaaa atacaaatac gctgccaaac ttgtttgact
     2281 ataaatttca tcggtatttt gattatgaac ctctttccga agaagatgcc acgcgaggaa
     2341 taatcggaat tccccgtgta cttaacatgt acgaaaatta tccattctgg tttacatttt
     2401 tcaccaaact cggattccgt gtcgtactct ctcctgcttc tacgagaaaa atttacgaac
     2461 tcggtatcga atccattcca agtgaatccg aatgttatcc ggcaaaactg gctcacggac
     2521 atattcaatg gctgatcaac aacggaatcg aacacatttt ctatccgtct gttccatatg
     2581 aaaggaatga atttgaagat tccaacaacc actacaactg tccgatcgtt acatcttatc
     2641 cggaaaatat aaaaaacaat atcgatccga tcatcgaaaa cgaagtcgac ttcatccatc
     2701 cgtttctttc ttttaaaaac gaagaaacca ttgcttatcg gttatttgaa gaactgggat
     2761 caaaattctc actgtcaaaa aacgagattt attccgcagt acacgatgca tggacagaac
     2821 ttgcttcctg ccgggaagat atgagacgca aaggggaaga aacgatccgg tttttgaacg
     2881 aaaccggaaa cagaggaatc gtccttgccg gaagaccgta ccatatcgac ccggaagtca
     2941 atcatggact tccggaaatg atcctttcct ataatattgc cgtactgact gaagattctg
     3001 tatcccatct tcatcccgtt gaacggcctc ttaatgttat ggatcagtgg atgtaccact
     3061 ctcgcttgta tgccgcggca aattatgtaa aaacaacaga aaaccttgat ttaatccaac
     3121 tgaactcttt cggatgcggt cttgatgctg ttactacaga tcaagttgcc gacatcttaa
     3181 caaattcagg taaaatttat acaactttaa agatcgacga agttaataat ctaggggcgg
     3241 ctcgtatccg tgtacgctct ctgctggctg ctatccgcgt cagagaacaa aaaaagacag
     3301 agcgcactat ccgctctgca gcaatcgaaa aagttccttt tacaaaggaa atgaaggcga
     3361 cacatactat tctttgtccg cagatgtctc cgattcattt tgaaatgctc gaagctgcct
     3421 ttagaggatc cggatatcgg tttgagattc tccctaacga caacaaacag gcagttgatg
     3481 taggcctgaa atatgtaaat aacgatgcct gctacccttc tctcatcgtt gttggacaga
     3541 ttatggaagc acttctttcc ggaaaatacg atcttgacaa agtggcagtt atcattactc
     3601 aaacaggagg aggatgccgc gcttccaatt atatcggatt tatacggcgc gccctgaaaa
     3661 aagcaggctt tggacacgtt cctgtcatct cactgaattt aagcggcctg gaaggaaatc
     3721 cgggatttaa actgaccccg tcccttatca tgcgtatcgt atacggcgct ctcttcggag
     3781 atatttttat gaaatgtgtc tatcatatgc ggccatacga agccgttccc ggaacaactg
     3841 atcagattca tcgcaaatgg gcaaagatct gcaaagactt tgtttcaaaa ggatatcctt
     3901 cccggcacaa attcaaaaag ctttgccggg acattattca cgactttgat tcgatcgaaa
     3961 tattggatat caaaaaaccc cgcgtcggaa ttgtcgggga aatacttgtg aaatttctgc
     4021 cggcagccaa taatcatctt gttgaactgc tagaggcaga aggcgcagaa gctgttgttc
     4081 ctgatcttgc agactttttg caatattgtt tttataacca aaacttcaaa gtctctcatc
     4141 tcgggatgaa gaagtcaaaa gcacttgcag gacgtctcgg tgttcaggcg gttgaatggt
     4201 ttcggtctcc tgccacaaaa g
//
LOCUS       DBMDXF010000166         4209 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5004_length_4209_cov_2.105891, whole genome shotgun sequence.
ACCESSION   DBMDXF010000166 DBMDXF010000000
VERSION     DBMDXF010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5004_length_4209_cov_2.105891"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="ACZPD4_06870"
     CDS             complement(<1..1022)
                     /locus_tag="ACZPD4_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1378817.1"
                     /translation="MTEKLFYKDSHLSKFDAEVLSCVRAKRQQVKDSFGRLDGKAGEY
                     YEIELDRTAFFPEGGGQYADTGVLYMADDDTCGLDETARKKVQVLDVRERNGRIFHIT
                     DGFIEAKTAVKGSIDWEERFMKMQQHTGEHIVSGLIHAAYGYNNVGFHLGSEDCTMDF
                     DGEITEEELRRIEAEANKAVWKNLKVFTHYPKKEELSQIEYRSKIEIEGQVRIIEIPG
                     YDCCACCAPHVTYTGEIGLIKLVQAQRYKGGVRITMLCGVRAFSDYVRKEAQAKEISR
                     LLCAKENEIADAVAHLAEETNQLKYELAEKERRLVKAYADMISESKDAVCIFTEEFGG
                     DSMRML"
     gene            complement(1030..2787)
                     /gene="argS"
                     /locus_tag="ACZPD4_06875"
     CDS             complement(1030..2787)
                     /gene="argS"
                     /locus_tag="ACZPD4_06875"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR1378818.1"
                     /translation="MLELITTEMEAAFEKAGYDKELAKVTLSNRPDLCEYQCNGAMAG
                     AKKYKKAPFMIAEDVAVYLKESSYFETVEVVKPGFINLKLAPQSVADYLNQMSETEKL
                     GVEEAEEPKTIMIDYGGPNVAKPLHVGHLRSAIIGESVKRITRFKGNKVIGDIHLGDW
                     GYQMGLIITELKKRKPELPYFDENFTGEYPAEAPFTIGELEEIYPTASSYAKEDEEYR
                     KEALHATYLLQNGHRGYRAVWNHIMNVSVTDLKKNYERLNVEFDLWKGEADAQKYIPD
                     MVQMLKEEGYARYDEGALVVDVQEESDKKEVPPCMILKSDGAALYDTTDLATLVQREE
                     DYQPDEVIYVVDKRQELHFTQVFRCAKKTGIVSPNTRLEFLGFGTMNGKDGKPFKTRE
                     GGVMRLENLIREINDEMYKKIMDNRTVEEEEAKKTARIVGLAAIKYGDLSNQAGKDYI
                     FDVDRFTSFEGNTGPYILYTIVRIKSILAKYKENGGSAEKLSIRPSGNVYEKALMLQV
                     LKFNDVFEAVCEETAPHKLCAYIYEIANEFNKFYHETKILSEEDEEKRSGYIALLGLT
                     KRVLETCIDLLGFEAPERM"
     gene            2944..3015
                     /locus_tag="ACZPD4_06880"
     tRNA            2944..3015
                     /locus_tag="ACZPD4_06880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2977..2979,aa:Arg,seq:ccg)
     gene            complement(3007..3918)
                     /locus_tag="ACZPD4_06885"
     CDS             complement(3007..3918)
                     /locus_tag="ACZPD4_06885"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR1378819.1"
                     /translation="MKIGSHVGMSGKEMLLGSAKEAVSYGADTFMFYTGAPQNTRRKE
                     IEELNIDPAWEYMKEHGIQEIIVHAPYIINLGNAVKPETFSLAVEFLAKEIDRTVSCR
                     SHTLILHPGAHVGEGVSIGTEQIIKGLNEVLTQDMDCCVALETMAGKGSEIGRTFEEL
                     ARIYDGVKYSDKLRVCFDTCHTSDSGYDIIHHFDDVIEEFDRIIGKDQIAVFHINDSK
                     NVPGAAKDRHENIGFGHIGFEAINYIVHHADFRDVPKILETPYIPSVENPKKSYAPYK
                     YEIEMLRAGKFDPDMKERIVKEHEKSV"
     gene            complement(3935..>4209)
                     /locus_tag="ACZPD4_06890"
     CDS             complement(3935..>4209)
                     /locus_tag="ACZPD4_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase PcrA"
                     /protein_id="HIR1378820.1"
                     /translation="RTYSSEVSGYKRSAYAQKPAFAAIQKGSGLMAKKSEGLSYGVGD
                     RVRHVKFGDGTVTEIKEGGRDYEVTVQFDTAGVRKMFALFAKLIKI"
BASE COUNT         1030 a         1145 c          692 g         1342 t
ORIGIN      
        1 atcaacatac gcatggaatc accgccgaat tcttccgtaa aaatgcaaac tgcatcttta
       61 ctttccgaaa tcatgtccgc atacgctttc actaggcgcc gctctttttc tgcaagttca
      121 tatttcagct gattcgtctc ttctgccaaa tgcgcaaccg catctgcaat ctcattttct
      181 ttcgcacaaa gaagcctgga aatttctttt gcctgcgctt cctttctcac ataatccgaa
      241 aacgcgcgga ctccgcacag catagtgatc cgcactccgc ccttataacg ctgcgcttga
      301 accagcttga tcagtccgat ctctcctgta tacgtcacat gaggagcaca gcaggcacag
      361 cagtcatacc ccggaatttc tataatacgt acctgaccct cgatctcgat cttactccga
      421 tattctatct gagacagttc ctccttcttc ggataatgcg taaatacttt caggtttttc
      481 cacacagctt tatttgcctc cgcctcgatc cggcgaagtt cctcttctgt gatctcaccg
      541 tcaaagtcca tcgtacagtc ctcactgcca agatggaagc cgacattatt atacccgtat
      601 gcggcgtgga tcagccctga aacgatatgc tctcccgtat gctgctgcat cttcataaag
      661 cgttcttccc agtcgatact tcctttcacc gcagttttgg cttcaatgaa accgtccgtt
      721 atatgaaaga tacgcccgtt cctttcacgg acatcgagca cttgaacttt ctttcgagcc
      781 gtttcatcca agccgcacgt atcatcgtct gccatatata gcactcccgt atcggcatac
      841 tgacctccgc cttccggaaa aaaggctgtc ctgtcaagtt ctatttcata atattcgcct
      901 gcttttccgt ccagcctgcc aaacgaatcc ttcacctgct gcctttttgc tctgacacac
      961 gaaagaactt ctgcatcgaa ctttgaaaga tgactgtctt tataaaacag tttttccgtc
     1021 atttttccat tacatccttt ccggcgcttc aaatccaagc aggtcaatgc acgtctccaa
     1081 cacgcgcttt gtcagtccga ggagcgcaat ataaccggaa cgtttctctt cgtcttcctc
     1141 ggagaggatc ttcgtctcat gataaaactt attaaattca ttcgcgatct cataaatgta
     1201 agcgcacaat ttatgcggag cagtctcttc gcatactgcc tcaaacacat cgttgaactt
     1261 caagacttga agcataagcg ccttctcata tacatttccc gacggacgga tcgaaagttt
     1321 ctctgcgctt cctccgtttt ccttatactt tgccaaaatg gacttaatac gcacgattgt
     1381 ataaagaata tacggacctg tatttccctc aaatgaagta aatctgtcta cgtcgaatat
     1441 ataatctttg cctgcctgat tcgacagatc tccatattta attgccgcaa gtccgactat
     1501 gcgggccgtc tttttcgctt cttcttcctc cactgtacga ttatccataa ttttcttata
     1561 catttcatcg ttaatttcgc ggatcagatt ttcaagacgc ataacgccgc cctcacgggt
     1621 tttgaacggt tttccgtctt tcccgttcat cgtaccgaat ccgagaaatt ccaatcttgt
     1681 attcgggctc acgatccccg tcttttttgc acagcggaac acctgtgtaa agtgaagttc
     1741 ctgacgttta tctactacat agataacttc gtccggctga taatcttctt ccctttgtac
     1801 aagtgttgca agatctgtcg tatcatagag cgccgctccg tctgacttta agatcataca
     1861 cggaggaact tcttttttat ccgactcttc ctgaacatcg accacaagag cgccctcgtc
     1921 gtaacgcgca tagccttctt cttttaacat ctgtaccata tcaggtatat atttctgcgc
     1981 gtccgcctcg cctttccaaa gatcaaattc tacattcagt ctctcataat ttttcttaag
     2041 atctgtaacc gaaacattca tgatgtgatt ccaaaccgct ctgtatcctc tgtggccgtt
     2101 ctggagcaaa tatgtcgcat gcaacgcttc tttcctgtat tcttcgtctt cttttgcata
     2161 tgaacttgct gtcggatata tctcctccag ttcgccgatc gtaaacggag cttccgccgg
     2221 atattctccc gtaaaatttt catcaaaata cggcagctcc ggtttgcgtt tctttaattc
     2281 cgtgatgata agtcccatct gatatcccca gtctcccaga tgaatatcac cgatcacttt
     2341 gtttccttta aaacgagtaa tccgttttac gctttcgccg ataattgcag aacgcagatg
     2401 tcctacatga agcggtttcg caacattcgg tccgccgtaa tctatcatga tcgtttttgg
     2461 ttcttccgct tcttctactc cgagtttttc tgtttctgac atttgattta aataatccgc
     2521 aacactttgc ggagcaagtt tcagattgat aaatcccggc tttacaactt ctaccgtctc
     2581 aaaataagaa ctttccttta aatataccgc cacatcttcc gcaatcataa acggggcttt
     2641 tttatatttc tttgctcctg ccatcgcgcc gttgcactga tattcgcaca aatcgggacg
     2701 atttgaaaga gttacttttg caagttcttt atcataaccg gctttttcaa aagccgcttc
     2761 catttccgtt gtgatcaatt ccaacattgt tttcaatttt tccacactcc tttatataga
     2821 aactctttat catatgaatg ctattctaac atgtatcagt tttcacggca acaaacaact
     2881 tgaaaacact ttacattttg cttactttat gctaggataa ttaaagttgt ttaacacaac
     2941 gatgcgtctg tagctcagtg gatagagcaa tgccctccgg aggcatgtgc gacggttcga
     3001 ctccgttcag acgctttttt catgctcttt tacaatccgt tctttcatat caggatcaaa
     3061 tttccctgcc cgaagcatct caatttcata tttataagga gcatatgatt tctttggatt
     3121 ttccacagat ggtatatatg gcgtctcaag tattttcgga acatccctaa agtccgcatg
     3181 atgaacgata taattgatcg cttcaaaacc gatatgtccg aatccgatgt tctcatgcct
     3241 gtcctttgca gcgcctggaa cattcttact gtcgttaata tgaaatacgg caatctgatc
     3301 ttttcctata atacgatcga actcttctat cacatcatca aaatgatgaa taatatcata
     3361 cccactgtcg ctcgtatgac atgtatcaaa acaaactctc aatttatcgc tatattttac
     3421 cccgtcgtaa atcctggcta attcttcaaa cgtacgaccg atctccgatc ctttccccgc
     3481 cattgtttct aacgccacac aacaatccat atcctgcgta agaacttcgt ttagtccttt
     3541 tatgatctgc tctgttccta tgctgactcc ttctcctaca tgggctcccg gatgcagaat
     3601 caacgtatgg cttctgcagc ttaccgtacg gtctatctcc tttgcgagaa actcaaccgc
     3661 aagagaaaat gtttccggct ttaccgcatt gcctaaatta ataatatacg gagcatgaac
     3721 aatgatttct tgaatcccgt gctctttcat atattcccat gcaggatcga tatttaattc
     3781 ttcgatttct ttccgcctcg tattttgagg cgctcccgta taaaacataa atgtatccgc
     3841 cccgtaagat actgcttctt ttgcagaacc aagcagcatc tccttaccac tcattccgac
     3901 atgtgaacct attttcacaa tgttatcctc caatctatat ttttataagc tttgcaaaca
     3961 aagcaaacat ttttctgact cctgcagtat caaactgcac cgtcacctca taatctcttc
     4021 caccctcttt aatctccgtc accgtgccgt ctccaaactt tacatgccgt acacgatctc
     4081 cgactccgta agagagtccc tccgactttt tcgccatcag tccgctcccc ttttggatcg
     4141 cggcaaatgc cggtttctga gcatacgcag atcttttgta tccactgacc tcggacgaat
     4201 acgtcctct
//
LOCUS       DBMDXF010000167         4203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5007_length_4203_cov_1.406631, whole genome shotgun sequence.
ACCESSION   DBMDXF010000167 DBMDXF010000000
VERSION     DBMDXF010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5007_length_4203_cov_1.406631"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            130..1032
                     /locus_tag="ACZPD4_06895"
     CDS             130..1032
                     /locus_tag="ACZPD4_06895"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR1378821.1"
                     /translation="MEYITGIEQYAGIEKTALTLGKFDGLHKGHQKLIDKICAYSGEN
                     CKSVLCAFDMHRESLMTKQERRSHLEGKIDCLIEYPFTKELREMEAEVFIKNILCEKL
                     HAAHIVVGTDFAFGHEKRGNTEMLKMFSEKGGYTLDVIEKERYDQIVISSTYIRDVLS
                     QGNVTLAAELLGYRYGVTGIVRHGRQLGRTLGFPTMNVEPEEKKILPRFGVYVCRINI
                     DGKWYNGIGNVGVKPTVTEEHKRLVEVFVYDYEGDAYGKEITVEFCDFERPEIRFSSV
                     NELKKQVAKDLRYGKDYFKASKEI"
     gene            1170..1436
                     /gene="rpsO"
                     /locus_tag="ACZPD4_06900"
     CDS             1170..1436
                     /gene="rpsO"
                     /locus_tag="ACZPD4_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1378822.1"
                     /translation="MIAKDKKQAIIKEYGRSEGDTGSPEVQIAILTARINELTDHFKA
                     NPKDHHSRRGLLKMVGQRRGLLAYLKKVDIERYRALIEKLGLRK"
     gene            complement(1668..3755)
                     /locus_tag="ACZPD4_06905"
     CDS             complement(1668..3755)
                     /locus_tag="ACZPD4_06905"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1378823.1"
                     /translation="MYKMYEMELAGRTLRVDVDRVAKQANGAVLMHYGDTTVLCTSTA
                     SEKPRDGIDFFPLSVEYNERLYAVGKIPGGFNKREGKASENAILTDRVIDRPMRPLFP
                     KDYRNDVTLENLVMSVDQDCSPELTAMLGASLATSISDIPFDGPISTTQVGLIDGEFV
                     FNPTAAQREVSDLALTVASTKEKVIMIEAGANEVPEDRMIEAIFAAHELNQKVIAFFD
                     TIVAECGKPKHEYESCAIPEELFDAIKEIVPPAEMEQAVFTDEKQIREDNIRQITEKL
                     EEAFIDNEEWLSLLGEAIYQYQKKTVRKMILKDHKRPDGRAIDQIRPLAAEVDLLPRV
                     HGSAMFTRGQTQICTVTTLAPLSEAQRLDGLDESETTKRYMHHYNFPSYSVGETKPSR
                     GPGRREIGHGALAERALLPVLPSEAEFPYAIRTVSETFESNGSTSQASVCASSMSLMT
                     AGVPIKAAVAGISAGLVTGDTDDDYLVLTDIQGLEDFFGDMDFKVAGTHKGITAIQMD
                     IKIHGLTRPIIEEAISATKKARTYIIDEIMNKAISEPRAEVGEFAPKIIQMQIDPQKI
                     GDVVGQRGKTINAIIEQTGVKIDIDDEGSVSICGTEKTAMEKAAKIIHTIVTDFEAGQ
                     VFEGKVISIKEFGAFLEFAPGKEGLVHISKIAKERVNKVEDVLSLGDVVKAVCLGKDK
                     MGRFSFSIRDVAE"
     gene            4015..4125
                     /locus_tag="ACZPD4_06910"
                     /pseudo
     CDS             4015..4125
                     /locus_tag="ACZPD4_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845147.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
BASE COUNT         1225 a          803 c          906 g         1269 t
ORIGIN      
        1 tcaaaaacaa aggcagttat tcagaatcga aaaaatgttt ttcgatcctg atggaaaata
       61 gaaaaattcg tttcaaatta tgtaaatgag atgagaatga tatgaagcgg ataaaaggaa
      121 gagtagatca tggagtacat cacggggatt gagcaatatg caggaataga aaagacagcg
      181 ttaactctcg gcaagtttga cggtcttcat aaaggacatc aaaaactgat cgataaaata
      241 tgcgcttatt cgggagaaaa ttgtaaaagc gtcctctgtg ctttcgatat gcaccgggag
      301 tcgttaatga caaagcagga gaggcggagt catcttgaag ggaaaatcga ttgtttgatc
      361 gaatatcctt ttacgaaaga actgcgtgaa atggaagcgg aagtgtttat taagaacatc
      421 ttgtgcgaaa aacttcatgc ggcgcatatt gttgtaggaa cagactttgc ttttggtcat
      481 gaaaaacgag gaaatacaga aatgttgaaa atgttttccg aaaaaggggg atatacgctg
      541 gatgtcatcg aaaaagaacg atatgatcag atcgtgatca gcagtactta tatcagagat
      601 gtcctttcac aaggcaatgt aactcttgcg gcagagcttc tcggatatcg gtatggagta
      661 accggaattg ttcggcatgg cagacaactg gggcggacgc tcggctttcc tacaatgaat
      721 gtcgagcctg aagaaaaaaa aatactgcca agattcggag tgtatgtctg ccgtatcaat
      781 atagacggaa aatggtacaa cggcattgga aacgtagggg tgaagccgac agttacggaa
      841 gaacacaagc gtcttgtcga agtatttgtt tatgattatg aaggagatgc atacgggaaa
      901 gaaattacgg tggaattttg tgattttgaa agacccgaga taagattttc ttctgtgaac
      961 gaattaaaaa aacaagtggc gaaagatctc cgttatggaa aagactactt taaggcttca
     1021 aaagaaatct aaaataaatt ctttacattg taagtggcat atgatatact acttgaggtt
     1081 agaaaaccag ccgatgttcg gtaatcggat cactccgacc gttacttaat atcaggcgtt
     1141 tataaaagta tttaaggagg agttacaaca tgatcgcaaa ggacaaaaag caggcaatca
     1201 tcaaagagta tggaagaagc gaaggtgata caggatcacc ggaagtacag atcgcaattc
     1261 ttacagcaag aatcaacgaa ctgacagatc acttcaaagc aaacccgaaa gatcaccact
     1321 caagaagagg tcttcttaag atggtaggac agagaagagg tcttcttgca tatcttaaaa
     1381 aagtagatat tgaaagatat cgtgcattga ttgagaaact cggactcaga aaatagtcag
     1441 aggtttcaat gtaagcaaaa aaggggcaga tttgatctgt tcctttttta tatgacggaa
     1501 agtctaattc cttgtctgga aggatcttag tgtgtacgac cgttttagaa aaagacccaa
     1561 tacagaggaa tcatcctccg tactgagtct caatttttaa tttgggcggg atgagggcga
     1621 ttgggaaatt ttgcctctac tacgctatct caacttgccc ttaattatta ttctgcaacg
     1681 tctctgatgc tgaagctgaa tcttcccatt ttgtcttttc caaggcaaac agcttttaca
     1741 acatctccga gagagagtac atcttcaact ttatttacac gttctttagc gatttttgag
     1801 atatgaacga gaccttcttt tcccggtgca aactcaagaa aagcgccaaa ttctttaata
     1861 cttataactt ttccttcaaa gacctgacct gcttcaaagt cggttacgat cgtgtgtatg
     1921 atcttcgcag ctttttccat tgctgttttt tctgtaccac aaatggaaac ggaaccttca
     1981 tcatcaatgt caatcttcac acctgtctgt tcgatgattg cgttaatcgt ttttccgcgc
     2041 tgtcctacaa catcgccgat cttttgcgga tcgatctgca tctgaatgat cttaggtgca
     2101 aattcaccga cttctgctct tggctcagaa attgccttat tcatgatttc gtcaatgata
     2161 taggttctgg ctttctttgt cgcagaaatt gcctcttcaa tgatcggtct tgtaagtccg
     2221 tgaatcttaa tatccatttg aatagctgta atacctttat gtgtaccggc tactttaaag
     2281 tccatatctc cgaagaagtc ttccagtccc tggatatcgg taagaacaag atagtcatcg
     2341 tctgtatctc cagttacaag acctgcggaa ataccggcta ctgcagcttt gatcggtact
     2401 ccggctgtca tgagcgacat actggatgcg catacgcttg cctgtgaagt agagccgttt
     2461 gattcaaatg tttcagatac tgtacggatg gcatatggaa attcggcttc gcttggaagt
     2521 accgggagaa gagctctttc tgccagcgca ccatgtccga tctcacgtct tccaggtcct
     2581 cttgacggtt ttgtttcacc tacagaatat gaagggaaat tataatggtg catatatcgc
     2641 ttagtcgttt ctgattcatc aagaccgtca agtctttgtg cttcagacaa aggcgccagt
     2701 gtcgttaccg tacaaatctg ggtctgtcct cttgtaaaca ttgcagaacc atggactctt
     2761 ggaagaagat ccacttctgc tgcaagaggt ctgatctgat cgatagcgcg tccgtccgga
     2821 cgtttgtgat cttttaagat catttttctt acagtttttt tctgatattg gtaaatggct
     2881 tcgccaagga gagagagcca ttcttcatta tctataaatg cttcttctaa tttttctgta
     2941 atctgacgaa tattgtcttc tcttatctgc ttttcgtctg taaatacagc ttgttccatt
     3001 tcggcaggcg gtacgatttc tttgattgcg tcaaacagtt cttcgggaat cgcacagctt
     3061 tcatactcat gtttcggctt tccgcattct gcaacgatcg tatcgaaaaa agcaatgact
     3121 ttctggttta attcatgtgc tgcaaaaatt gcttcgatca tgcggtcttc cggaacttcg
     3181 ttcgctccgg cttcgatcat aatcactttt tcttttgtag aagcaaccgt aagtgcgaga
     3241 tcagaaactt ctctttgagc tgccgtcggg ttaaacacaa actctccgtc aatgagtccg
     3301 acctgtgttg tagatatcgg gccgtcaaac ggaatatcgg aaatacttgt cgcaagagaa
     3361 gctccaagca ttgcggtaag ttccgggctg cagtcctgat ctactgacat tacaaggttt
     3421 tccaaagtta cgtcatttcg ataatctttc gggaaaagag ggcgcatcgg acggtcgata
     3481 actcggtcgg ttaaaattgc gttttcggat gctttccctt ctcttttatt aaatcctccc
     3541 gggatttttc cgacagcgta aagtctttcg ttatattcaa cactaagagg gaaaaaatca
     3601 attccgtctc ttggcttttc agatgccgtt gatgtacaca aaacagttgt gtctccgtaa
     3661 tgcatcagaa cagcgccgtt tgcctgtttg gcaactctgt ctacatctac acgcagtgtt
     3721 ctgcctgcaa gttccatttc atacattttg tacatgttat tctccttttc aatctatgtt
     3781 gtcatcaatc gtgaacggtt tttacaaatg tcccgaaact actgaaaaac atattttacg
     3841 aaaaatatat ttttcagtgg tctcggatac tttgaactgt ccataatctt atttattata
     3901 tcatacagtg ataacttagg caattatttt gaaagtcgaa aaggaatatg ttatatattt
     3961 ttttaaaatg ttcagactat aataagagat aaaaacagga gatgagtccg gaagatgaaa
     4021 atgaaaaaat taagtgaata tttgtttatg ggagcgttgg gaggaacgct ttattatatg
     4081 atagaaatta ttttcagagg atattctcat tggtcgatgt ttttgtaggc cgtttattgg
     4141 ctgatgtgtc cttcgaaagg cgaggcaggc aaaccggctg cgttgtataa gttagcatca
     4201 tcg
//
LOCUS       DBMDXF010000168         4201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5011_length_4201_cov_1.386341, whole genome shotgun sequence.
ACCESSION   DBMDXF010000168 DBMDXF010000000
VERSION     DBMDXF010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_4201_cov_1.386341"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="ACZPD4_06915"
     CDS             complement(<1..>1105)
                     /locus_tag="ACZPD4_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015662.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1378824.1"
                     /translation="LSDDEKKRYSLSDIVGKSGIEHTFDSVLQGEKGKTTFYVDNLGK
                     VTDTVSMTDPKAGNDVYLTIDKNLQISAYKLLEEKLAGIVLSKLSNVLDYDPSAEKDT
                     KYIKIPVGDAYNSFIANEIIDMKKFGRTDAKPAEQAVYNTFTQKKAEILSELMAQLQN
                     ENAPAYKDLSKEMKAYMDYICDTLLKQTTGILMSDKIEAEDETQIAWATQETISLNRY
                     LNYAISKNWIDTSKLGDSAYSSSEEIYSGVLAYLEEYLKEDSNFDKLLYKYLIKSGSV
                     TGAQICAIVYEQGVLPMDENAYNGLLNGTTDAYGWLYDKIKTLQITPGQLALEPCSGG
                     IVVTDPNSGDVLACVSYPGYDNNRLANTMDSAY"
     assembly_gap    1106..1115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1116..1775)
                     /locus_tag="ACZPD4_06920"
     CDS             complement(<1116..1775)
                     /locus_tag="ACZPD4_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1378825.1"
                     /translation="MSSRLIVLIIVFCLTSSILIGRLFYLQIVRGEDYLENYELQIRK
                     TRTVPGTRGIIFDRNGEVIAYNELAYSVTIEDIIPTDTKTEDKNKILNDTLDSVLSIV
                     EENGDSVIDNFGIILDSSGSYQFAETNETSRLRFVADVHGKSFIDDLTEKEKNKTAEQ
                     IVHYLCKRYGLDYSEHDAAYILKMVNMRYAMGLNSYQQWLTTVLASDVSDATAAAIME
                     NQ"
     gene            complement(1816..2349)
                     /gene="mreD"
                     /locus_tag="ACZPD4_06925"
     CDS             complement(1816..2349)
                     /gene="mreD"
                     /locus_tag="ACZPD4_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1378826.1"
                     /translation="MKRMKVKRIFITALVILVAYVLQCTVFPVLDIAGIKPNLLLIVT
                     ASFGFMRGSKSGMLVGFLSGLLIDIQFGKMIGFYALIYLVVGFLNGLFEQMFYDEDIK
                     LPLFLIAVSDFLYGITIYFFTFLLNSDFRFTYYLNRIIVPEMIYTIVITLGVYPLLLF
                     INHKLEAEEKRSASKFV"
     gene            complement(2396..3265)
                     /gene="mreC"
                     /locus_tag="ACZPD4_06930"
     CDS             complement(2396..3265)
                     /gene="mreC"
                     /locus_tag="ACZPD4_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1378827.1"
                     /translation="MKKKNQMSHTNRYILLGLSIFCVLMMVLSSFSDKVGGPFKTVAN
                     VTVIPLQQGMNQIGGWMGDMKDNFSTMKQLKSENKKLREQVDALTTENNYLQEERYEF
                     ERLQELYKLDQNYAEYEKTAAHVIGKDAGNWFGTFTIDKGSKDGIEVNMNVLAGSGLV
                     GIVTDVGPTWAKVRSIIDDSSNISAMAISTSDTCIVSGDMALMGTGQIAFSQMENNDN
                     VVAVGDQIVTSYISDKYLQGILIGSVSEVNVDSNNLTRSGYITPAVDFKNIQEVLVIT
                     TTKAELTGENKTE"
     gene            complement(3265..>4201)
                     /locus_tag="ACZPD4_06935"
     CDS             complement(3265..>4201)
                     /locus_tag="ACZPD4_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1378828.1"
                     /translation="VIAIKNKKNIFAIGDKAYEMYEKAPDDIEVVFPMKSGVIARFDN
                     MQTLLGTLLRTKKRSVWGSEYVVAVPTDVTEVEKKAFCDLVLHSEAKAKSVRIVERGL
                     ADAVGLGIDIFREKGIFIANFGGGITELSVLSSGGLVLNRLLKIGGDDFDSAITGLVR
                     HNKEFLIGKTTAEMLRKKFGVFEQSTNSTITVSGRDLVTGVPSQTDVSIGIVRAAIKD
                     PLDECVRAIKLMLDRTPPDVRRAIEEKGIYLTGGLTNLKGLPTYIRERVGLPVTTVDE
                     PELCAVKGLQKIILDKEYYKKLTYSMLDEDYRWLR"
BASE COUNT         1185 a          917 c          719 g         1370 t
ORIGIN      
        1 tagtaagcgg aatccattgt atttgccaga cggttattat catatcccgg ataagataca
       61 catgccagaa catctccgct atttggatct gtaactacaa ttccgccgga acacggctct
      121 aaagcgagct gcccgggcgt aatctgcaat gttttaatct tatcatatag ccagccataa
      181 gcgtctgtcg ttccgtttaa aagaccgtta tacgcattct catccattgg cagtacgcct
      241 tgttcataaa cgatcgcaca gatctgtgcg ccggtgacac ttcctgattt tataagatat
      301 ttgtagagaa gtttgtcgaa attactgtct tcctttaaat attcttcaag gtatgccaaa
      361 acaccgctat aaatctcttc agaactcgaa taagcgctgt ctcccaactt tgaggtatcg
      421 atccagtttt tggagattgc ataatttaaa taccggttca aactaattgt ctcttgcgtt
      481 gcccaggcaa tttgtgtttc atcttctgct tcgatcttgt ctgacatcag gattcctgtc
      541 gtttgtttta gaagagtatc gcatatatag tccatataag ctttcatttc ttttgaaaga
      601 tctttatatg ccggagcatt ttcgttctga agctgtgcca ttaattcgga aagtatctcg
      661 gcttttttct gagtaaacgt attatagaca gcctgttcgg caggttttgc gtctgttctt
      721 ccgaattttt tcatatcgat aatctcattt gcaataaatg aattatatgc atctcctacg
      781 ggaattttaa tatatttcgt atctttttcc gcagaaggat catagtctaa tacattgctt
      841 aatttgctta aaacgattcc ggcaagtttt tcttccagaa gtttataagc agaaatctgt
      901 aaatttttat cgatcgtaag gtagacgtca tttcctgctt tcggatctgt catacttaca
      961 gtatcggtta ctttcccgag attatctaca tagaatgtcg ttttaccttt ttcgccctga
     1021 agaacgctat cgaacgtgtg ctcaatgcct gattttccaa caatatccga caaagaatat
     1081 ctcttttttt catcatcgct tagatnnnnn nnnnnctgat tttccataat agccgctgcc
     1141 gtagcatcac tgacatcgga agcaagtacg gttgtaagcc attgctgata actattcagt
     1201 cccatagcat aacgcatatt gaccattttc agaatatatg cggcatcatg ttcggaataa
     1261 tccaatccgt atcgtttgca taaataatgt acgatctgct cggctgtctt atttttttct
     1321 ttttctgtaa gatcatcaat aaaacttttt ccatgcacat ccgccacaaa acgaagtctg
     1381 gatgtttcgt ttgtctcggc gaattgataa ctacccgatg aatcaagaat gattccgaaa
     1441 ttgtcgatca cactgtctcc gttttcttca acgatcgaaa gcacactgtc aagcgtatca
     1501 tttaatattt tgtttttatc ttcggtttta gtgtctgtag ggatgatatc ctcgatcgtc
     1561 acggaataag ccaattcatt gtaagcaatc acttctccgt tacgatcaaa aataatgcct
     1621 ctggttccgg gtacagttct tgtctttctt atttgcagct cataattttc caagtaatct
     1681 tctccgcgca cgatctgaag ataaaaaagt cgtccgatca atatcgaaga tgtaaggcaa
     1741 aagacaataa tgagaacgat caaccttgaa ctcatcaaat attcaacagc ttcttttatt
     1801 cgatctataa ttcgtctaaa caaatttact tgcactcctt ttttcttcgg cttcaagttt
     1861 atgattgata aacaaaagaa gcggataaac tccaagcgtt attacaatcg tgtaaatcat
     1921 ctcaggaacg atgatccggt ttaaatagta ggtaaaacga aaatcgctgt ttagcagaaa
     1981 tgtgaagaaa tatatagtga ttccataaag gaagtcactg accgctataa gaaacagggg
     2041 aagtttaata tcttcgtcat agaacatctg ctcaaacaag ccatttagga aaccaactac
     2101 aagatagatc aaagcgtaaa atccgatcat ctttccaaat tgtatatcaa tcaaaagtcc
     2161 cgataaaaat ccgacgagca ttccggattt tgaacctctc ataaatccaa atgacgcagt
     2221 gacgattaat agaagattcg gcttgattcc ggcaatatca agtaccggaa ataccgtaca
     2281 ctgaagtaca tatgccacga gaatgacaag cgccgttata aaaatgcgtt tgaccttcat
     2341 tcttttcata ttttaatctt cattcctttc tttatatgtc cgtctgtaaa gaaactcatt
     2401 ctgtcttgtt ttcacctgta agttctgctt ttgttgtcgt aataacaagc acttcctgaa
     2461 tatttttaaa atcgactgcc ggtgtaatgt atcccgaacg cgtcagatta tttgagtcta
     2521 catttacttc actgaccgaa ccgatcaaaa taccttgaag atacttatcg ctgatataag
     2581 acgtcacaat ctgatcgcct acagcgacta cattgtcatt gttttccatc tgggaaaaag
     2641 cgatctgacc tgttcccata agcgccatat ctccgcttac gatacatgta tccgaagtag
     2701 aaatcgccat tgcgcttata ttggaagaat cgtcgatgat cgagcgtact tttgcccagg
     2761 taggacctac atctgttacg atcccgacaa gtccgcttcc tgcaaggaca ttcatgttga
     2821 cttcaatacc gtctttactt cctttgtcta ttgtaaatgt accgaaccag tttccggcgt
     2881 ctttcccgat cacatgggcg gctgttttct catattcggc ataattctga tccagtttat
     2941 ataattcctg aagacgttca aattcataac gctcttcctg taaatagtta ttttctgttg
     3001 tcaaagcatc aacttgttcc ctcagttttt tattttctga ttttagttgt ttcatcgttg
     3061 agaaattgtc tttcatatct cccatccagc caccgatctg attcatccct tgttggagtg
     3121 ggattaccgt aacatttgca accgtcttaa acggaccgcc gactttgtca gagaaagagg
     3181 aaagcaccat cataagcaca caaaatatgg aaagcccaag caggatatat ctgtttgtat
     3241 gagacatttg atttttcttt ttcattacct taaccaccta taatcttcgt ctaacatcga
     3301 ataagtgagc tttttataat actccttgtc caaaatgatc ttttgaagtc ctttcacagc
     3361 acataattcg ggttcgtcaa cagttgtaac aggaagaccg acacgttctc ttatgtatgt
     3421 aggaagtcct tttaaatttg tcagtcctcc ggtaagataa attccttttt cttcgattgc
     3481 ccttcttaca tccggaggcg ttctatctaa cattagttta atcgcacgga cgcattcatc
     3541 aagggggtct tttattgcag cgcggacaat tcctatagat acatcggtct gcgaaggaac
     3601 accggtcaca agatctcttc ctgacacggt aatcgtactg tttgtgcttt gctcaaaaac
     3661 cccgaatttt ttacgaagca tttcagcagt tgtctttcct atgagaaatt ctttattatg
     3721 acgtacaagt cctgtgatcg cagaatcgaa atcatcaccg cctattttta aaagacggtt
     3781 taagacaagt ccgccggatg aaagaactga taattctgta ataccgccac cgaagtttgc
     3841 aataaaaatt cccttttctc taaaaatatc aatgcctaaa ccgactgcat cagccagtcc
     3901 tctttcgaca atacggactg attttgcttt tgcctctgaa tgcagaacca agtcgcaaaa
     3961 agcttttttt tcaacttccg taacatctgt aggaacggca acaacgtatt ccgatcccca
     4021 gactgaacgt ttttttgttc ttaacaaagt accgagcaat gtctgcatat tatcaaaacg
     4081 ggcgatcaca ccgcttttca tcggaaagac aacttcgata tcatcaggag ccttctcgta
     4141 catttcatat gctttgtcac cgatcgcaaa aatatttttt ttatttttaa ttgcaataac
     4201 g
//
LOCUS       DBMDXF010000169         4109 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5132_length_4109_cov_1.937375, whole genome shotgun sequence.
ACCESSION   DBMDXF010000169 DBMDXF010000000
VERSION     DBMDXF010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_4109_cov_1.937375"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="ACZPD4_06940"
     CDS             <1..257
                     /locus_tag="ACZPD4_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378829.1"
                     /translation="LLDEYDYETDIEVQREEAREEGRKMGREEGREEKRKEFLQKICS
                     LIQKKLEKGKTISEIADDLEDTEENISHLIEQFHLGRKES"
     gene            complement(308..1480)
                     /locus_tag="ACZPD4_06945"
     CDS             complement(308..1480)
                     /locus_tag="ACZPD4_06945"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR1378830.1"
                     /translation="MMIKREWRQLLEMIKFSLGKGKAPELRVETDWGKFYRICKFHKI
                     QSMVCPGIQKLPKEQQPPEEILKKFQEAESMEIARDAVQAFSQEELLEAFEKEGISVL
                     PLKGILMKRFYPVTSMRMMADLDILYETGRGKEVEAVLTDLGYYCDHRDSHHDVFFRK
                     PFMNIEMHHCMIGENSLLDAYYEDIWKRARQEEGKSHIYRLSWEDFYIFMIAHMAKHF
                     QGGGCGIRSIVDMWLFSDRMKESLDWDYVRKELEKIELAQFERCMCDLVSVWFEEKAE
                     TEFYAQLTELLMQSGIYGTITNYNIQHVAEVDKRVWKGQIKVWMEAIFLPYKAMKMQY
                     PYLEKYPVFLPAAWIQRIFRTCFCRKGRAGEVLSGMKVEGNEVRKRQDLFGKLGLS"
     gene            complement(1470..3164)
                     /locus_tag="ACZPD4_06950"
     CDS             complement(1470..3164)
                     /locus_tag="ACZPD4_06950"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378831.1"
                     /translation="MRVKMESKKGKSAVCWIYQYSKKSLWLVILLALISGAIAGSFIL
                     LALVSSSLLDVATGVKEGRLGVQIFLIVCLILLQAVLNILSSNVRIHATTKIEMQIRQ
                     GIFAAVLKKQYQHVSKIHSGEILNRLTSDIDIVVGAVVGLIPQAISMITKIVAGLAVL
                     FSIDARFTAAVLGIGLLVCIFSRIYSRHFKYLHKEVQRTNGVVRSYMQECIENLIVIK
                     SFANEHAVCGKLNQFQKDNYRIRIKRNAISNLANTLVYVLFTAGYYAALVWGAFQISF
                     GVMTFGTLTAFLQIIQQIRAPFRNVSGLIPQYYSMQASAERLMEIEDMEEESQKIRIE
                     DVSEFQKDFRAIVAEHVTFAYQNGDIILDDVSLRVNKGDLIAIVGESGIGKSTMMKLM
                     LHLMPCESGKLYFETVNGKIEIDAGTRNVFSYVPQGNMIMSGTIRENITFCNHDIGEE
                     EIRKAAEIACIWDYIETLPQGLETVLTERGEGLSEGQVQRIAIARAILNDAPILLLDE
                     CTSSLDKDTEWKVLQNLKKMNTKTIICISHTAAGVACCDRVVEIENKKFKEVSNDD"
     assembly_gap    3235..3244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3352..>4109)
                     /locus_tag="ACZPD4_06955"
     CDS             complement(3352..>4109)
                     /locus_tag="ACZPD4_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378832.1"
                     /translation="TYWLEEAGKLNSSDYTKDGWDIFRQIVTEATGVKDDATATEHEV
                     SEAVIALIDGMIALEKEKVYTVTVDGKKVAEGVYNQIVTITADAPQVGQKFAGWQLKD
                     KIVSYDEVYTFAISGNMAFTATYAPQEQTIEKPLYAAVANTMIIKRADGKANVKYIAK
                     LSIPDNYILNETGLLWYGKPDLENLCTETGPTPGTKKIVAPVISSTYQFAVNVNGIPA
                     GKTIRGVIYAKVTDKETQQTKWVYSEEVRLTNK"
BASE COUNT         1149 a         1070 c          616 g         1264 t
ORIGIN      
        1 tgttattaga cgaatacgac tatgagacag atatcgaagt gcaaagagaa gaggcaagag
       61 aagaaggaag aaagatggga cgagaagagg gcagagaaga gaaacgaaaa gaattcctcc
      121 aaaaaatctg ttctctcatt cagaaaaagc ttgaaaaagg aaagacaatt tccgaaattg
      181 cagatgatct tgaagatacg gaggaaaata tctcccatct gattgaacag tttcatcttg
      241 gaaggaaaga atcttaatta aatttggagc ctaaaccttt taaattgcaa aagtttaggc
      301 tcttgtttca tgacagtcct aattttccaa agagatcctg ccgttttctt acttcgtttc
      361 cctcgacttt cataccggag agaacttctc ctgctcttcc ttttctgcaa aagcaggtcc
      421 gaaaaatacg ctgaatccat gccgcaggaa gaaaaaccgg gtatttctcc agatacggat
      481 actgcatttt catcgcttta tacggcagaa atatcgcttc catccatact ttgatctgac
      541 ctttccaaac acgtttgtcc acttctgcca catgctgtat attataattt gtaatcgttc
      601 cgtatattcc gctttgcatc agcaattccg taagttgtgc ataaaactct gtttctgcct
      661 tctcctcaaa ccaaacggaa acaagatcac acatacaccg ttcaaactga gccagctcta
      721 ttttctccag ttctttccga acgtaatccc aatccagact ttccttcatc cgatccgaaa
      781 aaagccacat atctacgata gagcggattc cgcatccgcc gccctgaaaa tgtttcgcca
      841 tatgagcgat cataaagata taaaaatctt cccatgacag ccgataaata tgagatttac
      901 cctcttcctg acgcgcccgt ttccagatat cctcataata tgcatccaac agtgaatttt
      961 caccgatcat gcaatgatgc atctcgatat tcataaacgg ctttctaaaa aacacatcat
     1021 gatgactatc cctgtgatcg caataatatc cgagatccgt cagaaccgct tccacttctt
     1081 ttcctcttcc cgtctcatac aaaatatcca gatccgccat catccgcata cttgtaaccg
     1141 gataaaacct tttcatcaaa atgcctttta acggcaaaac agatatccct tccttttcaa
     1201 aagcctccaa caattcttcc tgtgaaaatg cctgcaccgc atctcttgca atctccatgg
     1261 actctgcttc ctgaaacttt ttcaatattt cttccggagg ctgctgttct tttggaagct
     1321 tctgtattcc ggggcatacc atagactgaa tcttatgaaa tttgcatatt ctgtaaaatt
     1381 tcccccagtc tgtctccact cttagttcag gcgctttccc ctttcccaaa ctgaatttta
     1441 tcatttctaa taactgacgc cattccctct taatcatcat ttgatacctc tttaaatttt
     1501 ttgttttcta tctccacaac tctgtcacag catgccactc ccgccgctgt atgcgaaata
     1561 cagataattg tctttgtatt cattttcttt agattctgga gaactttcca ctccgtatcc
     1621 ttatccaagg atgacgtaca ctcgtctaac aacaaaatcg gcgcatcgtt taatatcgct
     1681 cgggcaatcg caatcctttg aacctgccct tccgataatc cttctccccg ctctgtcaaa
     1741 accgtctcca agccttgcgg aagtgtctca atataatccc atatacacgc aatttccgct
     1801 gcttttcgta tctcttcttc tccgatatca tgattacaaa aggtaatgtt ctcccgaatc
     1861 gttcccgaca tgatcatatt cccctgcgga acataagaaa aaacgtttct cgttcccgca
     1921 tcaatctcaa tctttccatt taccgtctca aaataaagct ttccactctc acacggcatc
     1981 aaatgcagca ttaacttcat catcgtgctt tttccgattc ccgattcccc tacaatggca
     2041 atcagatctc ctttattaac acgcagagaa acatcgtcta atataatatc tccgttttga
     2101 tacgcaaacg tcacatgctc cgccactatc gcgcgaaaat ctttctgaaa ctccgacaca
     2161 tcttcaatcc gaatcttctg actttcctct tccatatcct ctatctccat cagccgctct
     2221 gccgacgcct gcatgctgta atactgtgga atcaaccccg acacattacg aaacggcgcc
     2281 cggatctgct gaataatctg caaaaaagcc gtcaatgttc cgaatgtcat cacaccgaat
     2341 gaaatctgaa acgctcccca tacaagcgcc gcataatacc ccgctgtaaa caaaacatat
     2401 acaagtgtat ttgccaaatt gctgatcgca ttacgtttaa tccgaatacg gtaattatct
     2461 ttttgaaatt gattcagttt gccgcatacc gcatgctcat ttgcaaatga ttttatgacg
     2521 atcagattct caatacattc ctgcatataa gaacgcacta cgccattcgt cctctgcact
     2581 tccttatgca aatatttaaa atgcctgctg taaatacggc tgaaaatgca aacaagaagc
     2641 ccgatgccca gcaccgcagc cgtaaaacgg gcgtcgatag aaaacagtac cgcaagtcct
     2701 gccacgatct tcgtgatcat ggaaatcgcc tgcgggatca gtccgacaac cgctcctacc
     2761 acaatgtcaa tatcacttgt cagcctgttt aatatctctc ccgaatgaat ctttgaaaca
     2821 tgctgatact gctttttaag taccgccgca aaaattccct ggcgaatctg catttcaatc
     2881 ttcgttgtcg catgtatccg gacgttactg ctcaaaatat tcaaaactgc ctgcagcaag
     2941 atcagacata caatcaaaaa aatctgaact cctaaccttc cctcttttac ccctgtcgca
     3001 acatctaata aactgctgga aacaagagcc agaagaataa aacttcccgc aatcgctccg
     3061 ctgataagcg ccaacagtat tacaagccaa agcgactttt tactatattg ataaatccag
     3121 cagaccgccg actttccttt cttactttcc attttcactc tcattcttat ttttctcatt
     3181 gcacttttct tatcactttt tgcctcttct acttcattct accttttcta ttttnnnnnn
     3241 nnnntttaaa aaagtttcat tattaaccct aaaaaagcag tgtcaaaata aagaaaaact
     3301 tttttgacac tgcctttttt atcttttcat aaaagaaccc tttttattat cttatttgtt
     3361 tgtaaggcgt acttcctcag agtaaaccca ttttgtttgc tgtgtttcct tatcagttac
     3421 ttttgcataa atgactccgc gaattgtctt tcctgccgga ataccattta cgttcactgc
     3481 aaactgatat gtagaactaa ttaccggagc aacgattttc ttcgtacccg gagtcgggcc
     3541 agtctctgtg caaagatttt ctaaatcagg ttttccatac cataacaacc cggtctcatt
     3601 caaaatataa ttatccggaa ttgacagctt cgctatgtat tttacatttg cctttccgtc
     3661 agctcgtttt ataatcattg tattagcaac tgctgcgtat aatggttttt cgattgtctg
     3721 ctcctgcggt gcgtatgtcg ctgtaaatgc catattgcca cttatcgcaa atgtatatac
     3781 ttcatcatat gaaacaatct tatcctttaa ctgccagcct gcaaacttct gtccaacctg
     3841 tggcgcatct gctgtaatcg ttacgatctg attataaaca ccttctgcaa cttttttccc
     3901 atcaacagta actgtgtaca ctttttcttt ttccagtgct atcattccgt caatcaatgc
     3961 aatgaccgcc tcgcttactt catgttccgt agccgtagca tcgtctttta ctccagtagc
     4021 ttctgttact atttgccgga atatatccca tccatctttt gtataatcag acgaattcaa
     4081 cttgcctgct tcctcaagcc aatatgtca
//
LOCUS       DBMDXF010000170         4098 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5148_length_4098_cov_1.861146, whole genome shotgun sequence.
ACCESSION   DBMDXF010000170 DBMDXF010000000
VERSION     DBMDXF010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5148_length_4098_cov_1.861146"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="ACZPD4_06960"
     CDS             complement(<1..227)
                     /locus_tag="ACZPD4_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378833.1"
                     /translation="MKAAISGETLGSVMELDEILSLFQKNGIHFIEIWPENIPVKVGK
                     TLLHKRHAAENAGKYPHLLFLPNLEEPSARN"
     gene            complement(240..1214)
                     /locus_tag="ACZPD4_06965"
     CDS             complement(240..1214)
                     /locus_tag="ACZPD4_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1378834.1"
                     /translation="MKKKMLAVAVCVSMCMMAFAGCTSESGGGSDKKAEQTEGKKEES
                     GKVFMTVSNQQNEFMVGMAENFKEVGEAAGYEVQLMDADLDATKQVSQIETAISENAE
                     AILVEPCSVDGLTTGLKEAHDAGIPVFVIHNNVSATDLVTSLIHVDVRQGGELKMEQV
                     IKDCGEDAKIAIMTGTLGQDTTNQICGGYDAVLEKYPNVEVVFEGAGNWGATDAAPLA
                     ENWLASGKEIDAIVCNNDGMALGVVPVLKSAGKSEQIKVYGLDATNEGLKAVDSGEMA
                     ATIYVDSKAEIEKAFEMLEDLKAGKKVDAEYKVPSVLVTKENVKEYMK"
     gene            complement(1241..2200)
                     /locus_tag="ACZPD4_06970"
     CDS             complement(1241..2200)
                     /locus_tag="ACZPD4_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1378835.1"
                     /translation="MKVLSKNSVMEFVKKTAILWVMIILILILSVISPSFFRGQNLLN
                     IIKQASITGVIGVGMTFVLITGGIDLSVGSVMALSGTMAASVAVAEKNMPIALVILVG
                     VGLGALCGLINGIGVSYIGFPPFIMTLGMMTIARGIPLVFTNGTPIFGLSEEFNMIAN
                     SRVLGVPSLVIFLVITVLAGHIILSKTVLGRRIYAVGGNEDAARLSGVSTAKVKLFVY
                     VFCGILSGIAGILICSRITSGNGTVAEGYEMNAISAAVIGGVSMTGGSGNVLGMVVGA
                     MILTIIQNSFDIMGVNSFYQNIIKGIIILLAVFLDLRGKKKKN"
     gene            complement(2197..3696)
                     /locus_tag="ACZPD4_06975"
     CDS             complement(2197..3696)
                     /locus_tag="ACZPD4_06975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1378836.1"
                     /translation="MEKLLEMKEICKSFGTNNVLKNVQFDLFAGEVHALIGENGAGKS
                     TLMKILMGMYKKDSGSLSVLGSDEAYTNPDQALKAGVAMIHQELNPIPDMSVAENIFL
                     GKEKRKWIFTSKKEQEKESKRYLDMLGIDLEPSVLMRELSVSEMQMVEIAKALSYGAR
                     IIIMDEPTSAITEAEVEKLFQNIKMLKEQGTGIIYISHKMDELFEISDRITVLRDGQY
                     VFSKKTKDLCPNDLIKAMVGREITEIYPESKSKIGEVILKVKHASRKGEFKKIDFELR
                     KGEKLGIAGLMGAGRTELMMAVFGAAKLEEGEIEIGGVPVRIQSPKDAIRQKIALVTE
                     DRKRYGLNLTASVEDNAVSVIESSLSKFGFYRRKLGRKAAEEMISRMNTKVTGMDQIV
                     GSLSGGNMQKVVLSKWLLNDPDIIIFDEPTRGIDVGAKAEIYKIIEELACKGKGIIII
                     SSEMPELIGLSQRIIVLHEGEQTGELRGKEMTQENIMTLASGYRKEEKE"
     gene            complement(3715..>4098)
                     /locus_tag="ACZPD4_06980"
     CDS             complement(3715..>4098)
                     /locus_tag="ACZPD4_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR1378837.1"
                     /translation="CFDVYGDKMSFEWEPTVDEGHTIFTGIDDFTKFTAPDTAELLPK
                     EIQKYTLRSAIKDPNQPSFIQGSGHGGSHPHLCNEFVNAIVEGRQPYMDAVRSANYTA
                     AGICAQESADHGGAEVEIPDFAEEF"
BASE COUNT         1105 a         1089 c          582 g         1322 t
ORIGIN      
        1 tcattccgcg ccgatggctc ttctaagttc ggtaagaata ataagtgggg gtacttcccg
       61 gcattctctg ctgcatggcg tttatgcaga agtgtttttc cgacttttac aggaatattt
      121 tccggccata tttcgataaa atgaatcccg tttttttgaa ataaagataa aatttcgtct
      181 aattccatca cacttccaag cgtctctccg gatattgctg ctttcataag actcacctgc
      241 tatttcatat attcttttac attctctttt gtaacaagca cagacggtac tttatactct
      301 gcatccacct ttttccctgc tttcagatct tcaagcattt caaacgcttt ttcaatctct
      361 gccttggaat caacatagat cgtcgctgcc atttctcctg aatcaactgc ttttaatcct
      421 tcatttgttg cgtccagacc gtaaacttta atctgttccg attttcccgc cgatttcaat
      481 acaggcacta ctccgagagc cattccatca ttgttgcaga cgattgcgtc aatttctttt
      541 cctgaagcca gccagttttc tgccaaaggc gcagcatccg tagctcccca gttgcctgcc
      601 ccttcaaata caacttctac attcgggtat ttttcaagaa cagcgtcata accaccgcag
      661 atctgatttg tcgtatcctg cccaagcgtt cctgtcataa tcgcaatttt agcatcttct
      721 ccacaatcct taataacctg ttccattttc aattccccgc cttgtcttac atctacatga
      781 atcagagatg tgaccaagtc ggttgccgac acattattat ggataacaaa caccggaatt
      841 cccgcgtcat gagcctcttt caaccctgtc gtcaacccgt ccacgctgca cggttcaacg
      901 agaatagctt ccgcgttttc agaaatagcc gtctcaatct gagatacttg tttcgtagca
      961 tcaagatcag catccatcaa ctgcacttca taccctgctg cctctccgac ctctttaaaa
     1021 ttttctgcca ttccgaccat aaactcattc tgttggttac ttactgtcat aaacactttt
     1081 ccggattctt cttttttccc ttctgtctgt tctgctttct tatccgaacc tccgccggat
     1141 tcacttgtac agcccgcaaa agccatcata cacatagaca cacatactgc cactgccaac
     1201 atctttttct tcatcatttt tcctccttaa aactcatttt ttaatttttc tttttctttc
     1261 ctcttaaatc caaaaatacg gcgagtaaaa taataattcc tttaatgata ttttgataaa
     1321 acgaatttac tcccataata tcaaaactgt tttgaataat cgttaaaatc attgccccga
     1381 caaccatgcc aagaacattt cccgatcctc cggtcatgct gacaccgccg attactgccg
     1441 ccgaaatagc gttcatttca tacccttccg ccacagtgcc attccccgac gtaattcttg
     1501 aacaaatcaa aattcctgca attcccgata aaattccgca aaaaacataa acaaacaact
     1561 ttacttttgc agtggacaca ccgcttaacc tcgctgcatc ttcatttcct cccactgcat
     1621 aaatccgtct tcccaaaaca gttttactca ggataatatg tcccgctaaa acagtgatca
     1681 caaggaaaat cacaagtgaa ggtactccta aaactcttga attcgcaatc atattaaact
     1741 cttcagatag tccgaagatc ggagttccgt ttgtaaaaac aaggggaatc cctcttgcaa
     1801 tcgtcatcat gcccaacgtc ataataaacg gcggaaatcc aatgtatgat acaccgattc
     1861 catttataag tccgcacagc gccccaagtc cgactccaac taaaataacc agagcaatcg
     1921 gcatattttt ttctgccaca gctacagatg cagccattgt tcccgataac gccattacag
     1981 atccgacgga aagatcaatc cctcctgtga tcaaaacaaa tgtcatccct acgccgatta
     2041 cccctgtaat agacgcctgc tttataatat tcagaagatt ctgccctctg aaaaagcttg
     2101 gactgataac agataaaata aggatcagaa taatcataac ccagagtatt gccgtctttt
     2161 ttacaaattc cataacagaa tttttactta aaactttcat tctttttcct cctttctgta
     2221 tcccgatgca agagtcataa tattttcctg cgtcatctct tttcctcgca attctccggt
     2281 ctgttctcct tcatgcaaca caataattct ttgacttaac ccgatcaact ccggcatttc
     2341 cgacgaaata ataatgattc cctttccctt acaggctaat tcctctataa tcttatatat
     2401 ctctgctttt gcgccaacat caattcctct tgtcggttca tcaaaaatga taatatcagg
     2461 atcatttaaa agccattttg aaagaacaac tttttgcata ttcccacccg aaagacttcc
     2521 gacaatctga tccattcccg taacttttgt gttcatacgg ctgatcattt cctccgcagc
     2581 ttttcttccc aactttcttc tataaaatcc aaatttggaa agactgctct caattacaga
     2641 aaccgcatta tcctcgactg atgccgtcaa attcaaaccg taacgtttcc tatcttcggt
     2701 cacaagcgct attttttgcc tgattgcatc tttcggactt tgtattctga caggaactcc
     2761 gcctatttca atctcacctt cttccagttt cgccgctccg aaaacagcca tcatcaattc
     2821 tgttcttccg gctcccatca atccggcaat tccaagtttt tcacctttcc gaagttcaaa
     2881 atcaatcttt ttaaactcgc ctttccgtga agcatgtttt actttaagga tcacttcgcc
     2941 gattttactc ttactttccg gataaatttc cgtgatctca cgccctacca tagctttgat
     3001 cagatcattc ggacaaagat cttttgtctt ttttgaaaat acatactgtc cgtctctaag
     3061 cactgtaatg cgatccgata tttcaaacaa ttcatccatt ttatgagaaa tataaatgat
     3121 ccctgttccc tgttctttaa gcatttttat attttgaaat aatttctcca cttccgcttc
     3181 tgtaattgca gaagtaggtt catccatgat aataattctt gccccataag aaagtgcttt
     3241 cgcaatttcc accatctgca tttcagaaac acttaattct ctcatcagta cagacggttc
     3301 cagatcgatt cccaacatat ccaaatatct ttttgactct ttttcctgct cttttttaga
     3361 cgtaaagatc cattttcttt tttctttccc aagaaatata ttttcagcaa cagacatatc
     3421 cggaatcgga ttgagttctt gatgaatcat ggcaactccg gctttgagcg cctgatccgg
     3481 atttgtgtac gcttcatctg atcccaaaac agaaagactt cccgaatcct ttttatacat
     3541 acccattaat attttcatta gtgtagattt tcctgctccg ttttctccga tcaaggcatg
     3601 aacttctccg gcaaaaagat caaactgcac attcttcagg acattattcg tcccaaaaga
     3661 cttacagatt tctttcattt ccagtaattt ttccataagt ccctcctaaa cagcttaaaa
     3721 ttcttctgca aaatccggta tttctacttc tgctccgcca tggtctgctg attcctgtgc
     3781 gcaaataccg gccgccgtat aatttgccga gcgaaccgca tccatatacg gctgtcttcc
     3841 ctctacgatc gcattcacaa attcattgca aagatgagga tgagaacctc catgcccgct
     3901 tccttgtata aaggacggct gatttggatc tttaatagct gaacgcaacg tatacttttg
     3961 aatctctttt ggcaaaagtt ccgctgtgtc cggagctgta aactttgtaa aatcatcaat
     4021 gcctgtgaaa atagtatgtc cttcgtctac cgtcggctcc cactcaaacg acattttatc
     4081 tccgtaaaca tcaaaaca
//
LOCUS       DBMDXF010000171         4044 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5224_length_4044_cov_1.616282, whole genome shotgun sequence.
ACCESSION   DBMDXF010000171 DBMDXF010000000
VERSION     DBMDXF010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5224_length_4044_cov_1.616282"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="ACZPD4_06985"
     CDS             complement(<1..674)
                     /locus_tag="ACZPD4_06985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1378838.1"
                     /translation="MRVAVVTGASSGIGREFVRQLEYFYKDLDEIWVIARREERLEQL
                     RKEVNVPLRIFAGDLQKKHVYRQFYTELCQWHPNIRMLVNAAGFGKSGTFSEIAEKGK
                     RIHTDMIDLNCTALIRITQMTLPYMKMGSRIINMASAAAFCPQPSFAVYAASKACVLS
                     FSRALGQELREKSIYVTAVCPGPVETEFFEVSGELKNPLKKLNMAAANKVVHRALIDS
                     RKKKSVS"
     gene            complement(687..2612)
                     /locus_tag="ACZPD4_06990"
     CDS             complement(687..2612)
                     /locus_tag="ACZPD4_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR1378839.1"
                     /translation="MNHDFLPVCREDMEKRGWKSVDFAYIIGDGYVDHPSFGHAIISR
                     LLESRGYRVGIISQPDWKDPESICIYGEPRLGFLVSAGNMDSMVNHYSVSKKRRRTDA
                     YTPGGEMGKRPDHACVVYGNLIRQKYKKTAVIMGGIEASLRRMAHYDYWSDKLKRSIL
                     LDSGADIVSYGMGERSILEIAEALEAGIPIEEITYIDGTVVKIKEKEKIYDAQFLPSY
                     EELKADRKMYAKSFYTQYLNTDAFSGKRLAEPYSEHLYVVQNPPSKPLSVTEMDDIYD
                     LPYMRTYHPSYKAKGGIPAISEIRFSLISNRGCFGGCSFCALTFHQGRIVQVRSHESL
                     IKEAEQMVEEKDFKGYIHDVGGPTANFRHPSCEKQLRHGVCATKQCLFPAPCKNIDAD
                     HSDYVALLRKLRKIPKVKKVFIRSGIRFDYLLADKKNTFLKELCQYHVSGQLKVAPEH
                     ISAPVLSLMGKPEHRIYEEFTRQFKKMNERLGMDQYLVPYLMSSHPGSTLKEAVELAE
                     YCRDLGYMPEQVQDFYPTPSTLSTCMYYTGIDPRTGQSVYVPKSPHEKAMQRALIQYR
                     DPKHYDLVLEALRKTGRMDLVGYGPKCLIRPGREKDSTNTGREKQVNTSKKPAYKKID
                     KKTNYKKKTIRNVHRKK"
     gene            complement(2623..3291)
                     /locus_tag="ACZPD4_06995"
     CDS             complement(2623..3291)
                     /locus_tag="ACZPD4_06995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1378840.1"
                     /translation="MLTGKKAVIFDMDGSLVDSMWIWPEVDRIYMDKYHLTAPDTFHR
                     DIEGMSYVETAQYFVDTFTTLNQTVEDVMQEWRDMTVELYATKVFPKAGAVEFLDLMK
                     QNGIRLGIATSNDREIAEAALNGRGLTKYFDSVRTSSEVAAGKPAPDVYLKVADDMNV
                     DPKNCLVFEDVINGILAGKNAGMEVCAVADDFTKADEAEKKAAADYFIRDFFDVLKNT
                     YERC"
     gene            complement(3285..>4044)
                     /locus_tag="ACZPD4_07000"
     CDS             complement(3285..>4044)
                     /locus_tag="ACZPD4_07000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1378841.1"
                     /translation="TEMGCGTRSQVKKEILKGSVSVNGEIAKKAERKVDTEKDEIIFK
                     GEKIFYAGYEYFMLNKPAGVVSATEDKKERTVIDLIKESKRKDLFPAGRLDKDTEGLL
                     LITNDGQLAHRLLSPKKHVDKIYYAKIKGIVTEEDVRLFEKGVHIGDEKPTMPAALEI
                     LSVQKERRKEKIKEQKILPETKNETDEKEDGYSEIYLTIKEGRFHQVKRMFRAVGKEV
                     VYLKRIQFGTLTLDPSLKPGQYRKLTEEEVEQLC"
BASE COUNT         1042 a          981 c          675 g         1346 t
ORIGIN      
        1 gaaacagatt tcttctttct gctgtcaata agagctctat gaacaacttt atttgccgcc
       61 gccatattca gctttttgag gggatttttt aattctccgc tcacctcgaa aaattctgtt
      121 tccacaggtc ccggacaaac tgccgtcaca taaatgcttt tttctcttaa ttcttgtccc
      181 agagcacgtg aaaaacttaa aacacatgct ttagacgccg cataaacagc aaaagaaggc
      241 tgcggacaaa acgccgccgc acttgccatg tttataatac ggcttcccat cttcatataa
      301 ggaagcgtca tttgcgttat gcggataagc gcagtgcaat tcagatcgat catatccgta
      361 tgaatccgtt ttcctttttc ggcaatttcc gaaaaagttc cgcttttgcc aaaaccggct
      421 gcattgacaa gcattcttat attagggtgc cattgacaaa gctctgtata aaattgtctg
      481 tatacatgtt ttttctgtaa gtctcccgca aaaatgcgaa gcggaacatt aacttctttc
      541 ctaagctgct ccaaccgttc ttcccttcgt gcaattaccc atatttcgtc gagatcctta
      601 taaaaatatt ccaattgacg aacaaactct cttcctatac ctgatgacgc tccggtgaca
      661 actgcaactc tcattttcat ttatcctcat ttctttctat gcacatttct gattgtcttt
      721 ttcttatagt ttgttttctt atctatcttt ttataagctg gctttttact tgtatttact
      781 tgtttttcac gtcctgtatt tgtactgtct ttttcacgtc cgggacggat cagacacttt
      841 ggtccgtagc cgacaagatc cattcttccc gtttttctca aagcttccaa aacaaggtca
      901 taatgtttcg gatcacgata ctgaatcaaa gcgcgctgca tcgctttttc atgcggggat
      961 ttcggaacat aaacagactg accggtgcgc ggatcgattc ccgtataata catgcaagtt
     1021 gaaagcgtag aaggtgtcgg ataaaaatcc tgcacctgct ccggcatata tccgaggtca
     1081 cggcagtatt ctgccagttc aacagcttct tttaacgttg atcccggatg agaagacata
     1141 aggtacggga caagatactg atccattccg agtctttcat tcattttctt aaactgtcgt
     1201 gtaaactctt catatattcg atgttccggt tttcccatca aagaaagcac gggagcgctg
     1261 atatgttcag gcgccacttt cagttgtccg cttacatgat actgacatag ttcttttaaa
     1321 aatgtattct ttttatctgc cagcaaataa tcaaatcgga ttcccgaacg gataaatact
     1381 ttttttactt tcgggatctt tcttaatttt cgtaatagtg caacataatc actatgatct
     1441 gcatctatat ttttgcaagg agcaggaaag agacactgct ttgtcgcgca tacaccgtgc
     1501 cgaagctgtt tttcacaaga tggatgacga aaatttgcgg tcggacctcc tacatcatgg
     1561 atgtatccct taaaatcttt ttcttcaacc atttgttccg cttcttttat aagcgattca
     1621 tgacttctga cctgtacaat acgcccctgg tgaaatgtaa gcgcacagaa actgcatcct
     1681 ccgaaacatc ccctgttgct gatcaggctg aagcggatct ccgaaattgc cgggattcct
     1741 cctttcgctt tataagacgg atgataagtc ctcatatacg gcagatcata aatatcatcc
     1801 atttctgtaa cacttaacgg ttttgacggc ggattctgaa cgacatataa atgttccgaa
     1861 taaggttctg caagtcgttt cccgctaaat gcatccgtat ttaaatattg tgtatagaag
     1921 ctttttgcat acattttccg atctgctttt agctcctcat aagaaggcag aaactgggcg
     1981 tcatatattt tttctttctc ttttatttta acgacagtac catcaatata agtaatttct
     2041 tcgattggta ttcccgcctc taaagcttcg gcaatctcca ggatcgagcg ttctcccatt
     2101 ccgtaagaaa caatatccgc accggaatca agaaggatcg accgttttaa tttatccgac
     2161 caatagtcat aatgtgccat acgtcttaaa cttgcttcga taccgcccat aattactgcg
     2221 gtctttttat atttctgtcg tatcagattt ccatacacaa cacaggcatg gtccgggcgt
     2281 tttcccatct ctccgcctgg ggtataagca tctgttcttc ttcgtttttt cgatacagaa
     2341 taatgattta ccatcgaatc catatttccg gcagaaacaa gaaatcccag tcgcggttct
     2401 ccgtaaatac aaatactttc cggatctttc caatccggtt gagaaataat tccgacacga
     2461 tatcctcttg attctaaaag acggctgatg atcgcatgac caaaagacgg atgatccaca
     2521 tatccgtctc cgataatata ggcaaaatca acgctcttcc acccgcgttt ttccatatct
     2581 tccctgcata caggtaaaaa atcatggttc atatttttca attcagcacc tttcgtaagt
     2641 gttttttaaa acgtcaaaaa aatctcggat aaaataatcc gcggcagcct ttttctccgc
     2701 ttcgtctgct tttgtaaaat catctgctac agcgcacact tccatgcccg cgttctttcc
     2761 tgccagaatt ccattgataa catcttcaaa tacaagacag tttttcgggt ctacattcat
     2821 gtcgtctgcg acttttaaat agacatccgg cgcaggtttc cctgccgcaa cttccgaaga
     2881 agtacgtaca gaatcaaaat atttcgtcag tccccgtccg ttcagcgcag cttctgcaat
     2941 ttcccgatca ttgcttgtgg caataccgag acggataccg ttttgtttca tcaaatccag
     3001 aaactcaact gcgccggcct ttggaaatac tttcgttgca tacagctcaa ctgtcatatc
     3061 ccgccattcc tgcattacgt cttctactgt ttgatttaat gttgtaaagg tatcgacaaa
     3121 atattgtgcg gtctctacat aactcatccc ctctatatct ctgtgaaatg tatccggtgc
     3181 agtcaagtga tatttatcca tataaatacg gtcaacttcc ggccagatcc acatcgaatc
     3241 tacaagtgat ccatccatat caaaaatgac cgcttttttt cctgtcaaca taattgttct
     3301 acctcttctt ctgttaattt tctatattgt ccgggtttta aagacggatc caatgtcaat
     3361 gttccaaact gaatcctttt taaataaaca acttcttttc ctaccgcacg aaacatacgc
     3421 ttcacctgat ggaaacgacc ttctttgatt gtaagataga tttccgaata tccgtcttcc
     3481 ttttcatctg tttcattttt tgtttcaggc aatattttct gctcttttat cttttctttc
     3541 cgccgttctt tttgtacaga aagaatttca agcgccgccg gcatcgtagg tttttcatct
     3601 ccgatatgta cgcctttctc aaacaatcgt acatcttctt ctgtcacaat tcctttgatt
     3661 tttgcataat aaattttatc cacatgcttt ttcggggaaa gaaggcggtg cgcaagctgc
     3721 ccgtcatttg taattaaaag aagaccttct gtatctttgt caagccgccc cgcgggaaat
     3781 aagtcttttc ttttgctttc tttgatcagg tctatgactg tacgctcttt tttatcttcc
     3841 gttgcagaca caactccggc aggcttattc aacataaaat attcataccc tgcataaaag
     3901 atcttttctc ctttaaatat aatctcatct ttttctgtat caactttccg ctcagctttc
     3961 tttgctatct ctccattgac ggaaacgctt ccttttaaga tctctttttt cacctgactt
     4021 cttgtgccgc atcccatttc cgtc
//
LOCUS       DBMDXF010000172         3991 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5296_length_3991_cov_1.095116, whole genome shotgun sequence.
ACCESSION   DBMDXF010000172 DBMDXF010000000
VERSION     DBMDXF010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_3991_cov_1.095116"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            158..892
                     /locus_tag="ACZPD4_07005"
     CDS             158..892
                     /locus_tag="ACZPD4_07005"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0032267 - tRNA(Ile)-lysidine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase"
                     /protein_id="HIR1378842.1"
                     /translation="MQLQRLLSYTRKAVDEYSMIQEGDHIAVGISGGKDSLTLLYALH
                     GLKRFYPRKFDLSAVTVDLGFADFNLEPIQNLCRELEVPYKIVKSDIYDILFNVRQES
                     NPCSLCAKMRKGALNEAVKEMGCNKVAYAHHKDDIIETMLLSLIFEGRFYSFSPKTYL
                     DRMDLTVIRPMMFIDEMEVIGFRNKYSLPVVKGKCPVDGHTKRQYAKELLRELNKEHP
                     GTKERMFHAILTGDIKGWPQRTISRR"
     gene            complement(999..>3991)
                     /locus_tag="ACZPD4_07010"
     CDS             complement(999..>3991)
                     /locus_tag="ACZPD4_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1378843.1"
                     /translation="AAATAKNKLTPFFDPDNTNVIPSFYAVLAGVKRTDDTVSAKLFD
                     KDPSTAGEWNQNQKIGDYYGVDLGRVIPVTDITILQGASETDHDYFHNFVLEYSETGE
                     DGSWVTIQEYKETDESHKIEKTGLNINARYVRLRLTKTGTTTPNKANYWTKVREFTVN
                     KKFTDGERIYTNIEKLKETPIHIYENEFRIRDIKDLELKPNEYVGIKMTSLVSVSDIF
                     SESDEFEKAKIQYSTNGIEWTDAKNFKGPQPMRYVRLVNQTNETLKGSLTKLGISVNN
                     LKITPKMSETSFTNPLKEGTWENMFDGDFTTHAWTNQGQNNGGYIIIDLGTSTPLYDV
                     TIVTADGKPHLYDADIQISSDKKEWTTIASVKNDNTKVEPPYRYVRANAQGAKSRYLR
                     ILVTDDVIGKYDNPFLCIKEIEFNKNIEENTSTYPIASSAEGNLEKCIDQDLSTVFTT
                     ENKTAGWLEYKLTDNTKLKSFSILQDVHTISEANVKVRTGTDKWKDLGTFDKGTKTFD
                     VSEYDNIFAIRLEWAEGKAPSIYEIYTNEDEKQNTDDIGIYVEQIIDTEADSEKPKPE
                     QPEIPDSENLAINRPVEVSGTELDRDQKDFAVDGNRETRWNSNYIKGDHASEEAWIIV
                     DLGEETNIIESLTAEYFNLVYPTDYDVLVSADKENWITAKTIKNEPNGAAHPTDEISL
                     DQPIIGRYVKLLFRELNSAAVGNCVGLEELTITGKRISSETELKEYMPISDIKIDKGT
                     SQEELRKLLPLIISGKLALKDSDKILDLASLFVNWDLSEYTSAEDTILTLKGNLIGAG
                     KATGDEISLNVIIGTGSTEPPVDPEGPTDPEGPTDPEGPTDPEGPTDPEGPTDPEGPT
                     DPEGPTDPDTPDSGKIEQLKQELALLIETAQAKVESGKYTDETVKILKEQIIKSEALL
                     TDNVTAEQLEQQINSIKKSINGLTEKKADLETSDRPNQNITHSEKSTAVKTGDSTHII
                     PLFLLMICTGFSLILFRKRNTYNK"
BASE COUNT         1060 a          723 c          762 g         1446 t
ORIGIN      
        1 tattactatt tttaacttac ggatcgtaat ctgatttttt tcataaacaa ccggtgattc
       61 tatgattttc tgtaagtttt cgacttgtat ttatattctt ctgccgatat aatataaacg
      121 ttataattac agatcaatca tgaaaggtag gaagaaaatg cagttacaac ggttactcag
      181 ttatacgcgt aaagcagtgg atgaatatag tatgatacag gaaggggatc atattgcggt
      241 aggtatttcg ggcggaaaag acagccttac cctgctctat gcgcttcatg gattaaaacg
      301 gttttatccc cgtaagtttg atttaagtgc tgtaactgta gatcttggat ttgcagattt
      361 taatttggaa ccgattcaaa atttatgcag ggaattagaa gttccttata aaatagtaaa
      421 atcagatatt tatgatattc tttttaatgt acgccaagag agtaatcctt gttcgctttg
      481 tgcaaaaatg cgtaaaggcg ccttaaatga ggcggttaag gagatggggt gtaataaagt
      541 ggcttatgct catcataaag atgatattat tgaaacaatg ttgctctcat tgatttttga
      601 aggaagattt tattcctttt ctccaaaaac atatttagat cgtatggatc ttactgttat
      661 ccgtcctatg atgtttatag atgaaatgga agtaatcggt tttcgcaata aatattctct
      721 tccggttgta aaaggaaaat gtcctgtaga cggacatacg aagcggcaat atgcaaaaga
      781 acttttaaga gaacttaaca aagaacatcc gggaacaaaa gaacgaatgt ttcacgctat
      841 tttaacagga gatattaaag gttggccgca gcgcacgata tcgagacgtt aaataagatc
      901 ttaaaagtga ataaactagc ggcaagtaaa acacatccga tgtttaatcc atcggatgtg
      961 tttttataat atgtgacatt atcgcatgtc aacacctatt atttattata agtatttctt
     1021 ttcctgaata ggatcaagga aaatccggtg caaatcatca gcagaaatag aggtattata
     1081 tgagttgagt cacctgtttt tactgctgta cttttttctg aatgagtgat gttttgattt
     1141 ggccggtcag aagtttctaa gtcggctttt ttttcggtta atccgttgat tgatttttta
     1201 atactattga tttgctgttc aagttgttcg gcagtgacgt tgtctgttaa aagcgcctca
     1261 gattttatga tttgctcttt aagtattttg actgtttcgt ctgtgtattt accggattcc
     1321 acttttgctt gagcagtttc aatgagtaga gctaattcct gttttaattg ttcaatcttt
     1381 cctgaatcag gtgtatccgg atcagtagga ccttccggat cagtagggcc ttccggatca
     1441 gtaggacctt ccgggtcggt aggaccttcc gggtcggtag gaccttccgg atcagtagga
     1501 ccttccggat cggtaggacc ttcagggtca acaggaggtt ctgttgatcc ggtgcctatg
     1561 atcacattta gggaaatctc gtctccagtt gcttttcctg caccgatcaa attccctttt
     1621 aaagtgagga tcgtatcttc agcagaagta tattctgaaa gatcccagtt tacaaataat
     1681 gatgccaagt ccagtatttt atcactgtct tttaatgcaa gctttccact gataattaaa
     1741 ggaagcagct ttcttaattc ttcttgtgac gtacctttgt cgattttaat atcgctgatc
     1801 ggcatatatt cttttaattc tgtttcggag gatatcctct ttcctgttat ggtaagctct
     1861 tctaaaccta cacagtttcc gacagcagca ctgtttaatt cacggaataa taatttgaca
     1921 taccttccga tgattggttg atcaagtgaa atttcatctg ttggatgggc tgctccgttc
     1981 ggttcgtttt ttatcgtttt tgcagtgatc cagttttctt tatcagcaga tacgagaacg
     2041 tcatagtctg tcggataaac taaattaaaa tactctgcag taagtgattc gatgatattt
     2101 gtttcttcac cgagatcaac gatgatccat gcttcttcag atgcatgatc gcctttgatg
     2161 taattgctgt tccatcttgt ctcacggttc ccgtccacgg caaaatcttt ttgatctctg
     2221 tcaagctcgg ttcctgatac ctctaccgga cggtttatag caagattttc tgaatccggg
     2281 atttcaggtt gttcaggctt tggtttttcg gaatctgctt ctgtatcgat gatttgttct
     2341 acatagattc cgatatcatc tgtattttgt ttttcatctt cgtttgtata aatttcatat
     2401 atagaaggcg cttttccctc tgcccattca agtcgaatcg cgaaaatatt gtcatattct
     2461 gatacatcaa aagtttttgt tcccttatca aatgtaccta agtctttcca tttatcagtt
     2521 ccggttcgta cctttacatt agcttcggaa atagtatgta catcttgcaa aatagagaac
     2581 gattttaatt ttgtattgtc tgtcagttta tattccagcc atccggcagt tttgttttct
     2641 gttgtaaata cagtggacag atcctgatcg atacattttt caagattacc ttctgcactc
     2701 gaagcaatcg gatatgtact tgtattttct tcaatatttt tgttaaattc aatttctttg
     2761 atacaaagga acggattatc atatttaccg ataacgtcat ctgttacgag aatacgaaga
     2821 tatctgctct tagctccctg ggcatttgct cttacataac gatagggagg ttccactttt
     2881 gtattgtcat tttttacaga cgcgatcgtg gtccactctt ttttatcaga ggagatttgt
     2941 atatctgcat cgtataaatg aggttttcca tccgctgtga cgattgtaac atcataaagc
     3001 ggtgttgaag ttccgaggtc tataataatg tatccgccat tattttgacc ctgattcgtc
     3061 caggcatggg ttgtaaaatc tccatcaaac atattctccc acgtaccttc tttaagcgga
     3121 tttgtaaaac ttgtttcgct catcttcggt gtgatcttaa gattatttac ggaaatacct
     3181 agttttgtca atgatccttt tagcgtttca tttgtctgat tgacaagacg gacatatcgc
     3241 atcggttgtg gccctttgaa atttttcgca tctgtccatt caattccatt tgttgaatat
     3301 tgtatttttg ctttttcaaa ttcgtctgat tcggaaaaaa tatcagacac tgagacaagg
     3361 gaggtcattt tgataccgac atattcattc ggttttaatt ctaaatcctt aatgtctctt
     3421 atacgaaatt cgttttcgta aatgtggatc ggagtctctt ttaatttttc aatatttgta
     3481 taaatacgtt ccccgtcagt aaatttctta tttactgtaa attcgcgtac ttttgtccaa
     3541 taatttgctt tgttcggagt tgtcgttcct gtcttggtaa gacgcagacg cacatagcgt
     3601 gcatttatgt ttaaccctgt tttttctatt ttatgtgatt catctgtttc tttgtactcc
     3661 tgaattgtga cccatgaacc gtcttcacct gtttcagaat attctaaaac gaaattgtgg
     3721 aaatagtcat gatcagtttc tgatgctccc tgaagaattg taatatctgt tacagggata
     3781 acacgaccta aatctacgcc atagtaatct cctatctttt ggttttgatt ccattcgccg
     3841 gcagttgatg gatctttgtc aaataatttt gcggaaacag tatcatctgt tcgttttaca
     3901 cctgcaagaa ctgcataaaa cgaaggaatt acattcgtat tatcaggatc gaaaaacggt
     3961 gttaatttat tttttgccgt tgcggctgct t
//
LOCUS       DBMDXF010000173         3926 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5383_length_3926_cov_1.859608, whole genome shotgun sequence.
ACCESSION   DBMDXF010000173 DBMDXF010000000
VERSION     DBMDXF010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5383_length_3926_cov_1.859608"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..912)
                     /locus_tag="ACZPD4_07015"
     CDS             complement(127..912)
                     /locus_tag="ACZPD4_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1378844.1"
                     /translation="MAILEVEHLSKSFDRTEVLKDITFSLEKGQVLSIIGSSGSGKTT
                     LLRCLNFLERPDGGSISVNGETLFDIHDPITSQEGEIRKKRLHFGLVFQSFNLFPQYT
                     ALKNVMLAKELLAKEQPDYKQHKKEIHEEIEKEARRLLTQMGLADRMENYPHQLSGGQ
                     CQRVAIARALALKPDILCFDEPTSALDPELTGEVLKVIQSLADQETTMIIVTHEMAFA
                     RDVSSQVIFMDGGCILEQGTPEEVFENPKEERTKQFLSRFTNG"
     gene            complement(925..1611)
                     /locus_tag="ACZPD4_07020"
     CDS             complement(925..1611)
                     /locus_tag="ACZPD4_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1378845.1"
                     /translation="MDLILEQFPIVFKSLNIGFLQTLKLFFVTLLGAVPLGLVIAFGS
                     MSRFKPLSYLTKIIVWIIRGTPLMIQLLIIYFFPGLVMGNNIWGGGEAGRFLAAAVAF
                     IFNYACYFSEIYRGGIQGVPHGQEEAGLVLGMTRSQIFFKVTLLQMVKRIVPPMSNEI
                     ITLVKDTSLARIIALQEIIWAGQAFLKGSQGIAGVIWPLFFTAVYYLIFNGILTVLLG
                     RLEKKLDYFK"
     gene            complement(1639..2454)
                     /locus_tag="ACZPD4_07025"
     CDS             complement(1639..2454)
                     /locus_tag="ACZPD4_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1378846.1"
                     /translation="MKRRVLSVLLCAGLVVSVLAGCGSDDAKKDSGKKAASESDLDYV
                     KEKGTLVVGITNFEPMDYQDEEGEWIGFDADMAKAFGESLGVDVEFVEIDWDNKILEL
                     DGKTIDCVWNGMTLTEEVTSSMECTDAYLNNAQVVVLPADKADKYQDEKSLSDLSFAV
                     ESGSAGEDEVENRGLDFTPVTAQADALMEVAAGTSDAAVIDLLMATAMIGEGTNYADL
                     AYTVRLSEEEYGVGFRKGSDLAKALNDFFAECYEDESIVKCAEKYNLQDMLVK"
     gene            complement(2634..3401)
                     /locus_tag="ACZPD4_07030"
     CDS             complement(2634..3401)
                     /locus_tag="ACZPD4_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378847.1"
                     /translation="MGNLILCHDRHAAHPYEITRIHCRIFTIEELCYYLCNNLYLIDY
                     TIMNGQLCDWLDEELEMKALASELRDVLRLHGSVEKFVLTILKSSRLYREPEMIRIQN
                     VLEHLKNQKDIERQKFKGDNLLESGEVEEAILVYQAILNGEKDETVDEKFYGKIYASL
                     GAAYGRLFLYQESAKMYDCAYQICGDKTLIKPYLYASYKYMSLEEYHILITKSEEYME
                     INAQMRREVDKINQEVSVEPEPGIMEKWKRSHRRSHA"
     gene            complement(3376..>3926)
                     /locus_tag="ACZPD4_07035"
     CDS             complement(3376..>3926)
                     /locus_tag="ACZPD4_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HIR1378848.1"
                     /translation="LTGEGFENNWYPKALRVLCNGRRAFIGNNLYSKGACYTAYRKLF
                     MHIENPVYLSEDKLTDQITVNMRVGGQEMWYPIVSWGNHWYESNNQWEVLMEDVEDIE
                     LRIESLVQGNVRTETLSLDGFPKRAEYSMRLQLETLFLDEKTCRISVRDAGFGDFFPA
                     TDFHAEKTIHLGGNDGKFNSLS"
BASE COUNT         1086 a         1052 c          617 g         1171 t
ORIGIN      
        1 aaaatcaaca taccctccgc cttgtatttc tgtcatctca ttattttatt cattgccatt
       61 taataagttt ttcttataaa caaagtatta tttattactt ttttataaaa aacattattt
      121 agaaactcat ccattagtaa aacgagacaa aaactgcttc gttctctctt ctttcggatt
      181 ttcaaaaact tcctccggag tcccctgttc caaaatacat cctccatcca taaaaatcac
      241 ctggctggac acatctcttg caaacgccat ttcatgtgtg acgatgatca tcgtcgtctc
      301 ctggtctgca agactttgga tcactttcaa cacttctccc gttaattcag gatcaagcgc
      361 cgacgtcggc tcatcaaaac ataagatatc cggctttaac gcaagggctc tggcgattgc
      421 gacacgctga cactgtccgc cggaaagctg atgtggataa ttttccatac gatccgcaag
      481 tcccatctgg gtcagaagcc tccttgcctc tttttcaatc tcctcatgga tctctttttt
      541 atgttgttta taatccggtt gttcttttgc gagaagctct tttgccaaca ttacattttt
      601 cagagccgta tactgcggga acagattaaa cgattgaaat accaacccga aatgaagccg
      661 ttttttccga atttctccct cctgtgaagt tatgggatca tgaatatcaa acaatgtttc
      721 tccgtttaca ctgatcgatc caccgtccgg tctttccaga aaattcaaac acctaagcag
      781 cgtcgtcttt ccgcttcccg aagaaccgat aattgaaaga acctgtcctt tctcaagaga
      841 aaaggtgatg tctttcaaca cttccgttcg atcaaaactt ttactcaaat gttctacttc
      901 aagaattgcc acacgctcca cctcctactt gaaataatcc agtttcttct caagacgtcc
      961 cagaagcact gtcaaaatac cgttaaagat cagataatat accgctgtaa aaaagagcgg
     1021 ccagatgact ccggcaattc cctgcgatcc tttcagaaat gcctgtcctg cccatataat
     1081 ttcctgaagc gcaataatgc gggcaagaga cgtatctttc acaagagtaa tgatctcatt
     1141 agacatcgga ggtacgatcc gttttaccat ctgaagcaaa gttaccttga aaaaaatctg
     1201 acttcgcgtc atgccaagca caagccccgc ctcttcctga ccatgcggta ctccctgaat
     1261 accgcctctg tatatttctg aaaaataaca tgcgtaatta aaaataaatg caactgccgc
     1321 cgcaagaaaa cgtccggctt ctcccccgcc ccagatattg tttcccataa caagtcccgg
     1381 aaagaaatag atgatcaaaa gctggatcat aaggggcgtt cctcttatga tccacacaat
     1441 aatcttcgtc agataactaa gcggtttaaa ccgcgacatc gatccgaatg caatgacaag
     1501 cccaagcgga accgctccga gcagtgtcac aaaaaacaac tttaatgtct ggagaaatcc
     1561 gatattcagt gatttaaaca caatcggaaa ctgttctaat atcaaatcca ttttttgtct
     1621 acaactccgt cttttatctt atttcacaag catatcctga aggttatatt tttccgcaca
     1681 tttcactatg ctttcgtctt cataacactc tgcgaagaaa tcgttcagag cttttgcaag
     1741 atcagaacct tttctgaatc caacgccgta ctcttcctcg cttaatctta ccgtataagc
     1801 aagatccgca taatttgttc cttctccgat catcgccgtc gccatcagaa gatcgatcac
     1861 agccgcatcc gacgtacctg ccgcaacttc catcaacgca tccgcctgtg ctgtcaccgg
     1921 agtgaaatca agtccgcgat tttccacttc atcttctccc gcgcttcccg attctaccgc
     1981 aaacgacaga tctgacaggc ttttctcatc ctgatattta tctgctttat ccgccggaag
     2041 tacaacaacc tgtgcattat ttaagtatgc gtctgtacat tccatagaac ttgtaacttc
     2101 ttcggtaagc gtcattccgt tccatacaca gtcgatcgtc ttcccgtcaa gttctaagat
     2161 cttattatcc cagtcaatct ccacaaactc tacatctacg ccgaggctct ctccgaacgc
     2221 cttcgccata tccgcatcaa aaccgatcca ttcgccttct tcatcctggt aatccatcgg
     2281 ctcaaaattt gtgattccca cgacaagcgt tcctttttcc tttacataat cgagatcgct
     2341 ttctgaagct gctttctttc ccgaatcttt ctttgcatca tcagaaccac atcctgccaa
     2401 cactgaaaca acaagtcccg cacacaacaa aacacttaac actcttcttt tcatagataa
     2461 cctccacaac caaacttata gttctcgctt tctttcttta ctaatgagtc actgtaccat
     2521 agtaaagtta cctctgtatt ctaacatgga gaaccctccg tgtcaacaca cagagggtct
     2581 ttcttctcac cgattgtaac agaccagatt gtaatctgat ctgccaatat atattatgca
     2641 tgacttcttc tatgacttcg tttccacttt tccatgattc cgggttccgg ttctacagac
     2701 acctcttggt tgatcttatc tacttccctg cgcatctggg cgtttatttc catatattct
     2761 tcgctttttg taatcaaaat atggtattct tcaagcgaca tatatttgta cgacgcataa
     2821 agatacggct tgatcaacgt cttatccccg cagatctgat atgcacaatc atacattttc
     2881 gccgactctt gatagaggaa caaacgaccg tatgccgctc cgagacttgc ataaatcttt
     2941 ccgtaaaact tctcatctac agtctcatct ttttccccgt ttaaaatagc ctgatataca
     3001 agaatcgcct cttccacttc cccgctttca agaagattgt cgcctttaaa tttctgccgt
     3061 tcaatatctt tttgattttt caaatgttcc aaaacattct gaatacgaat catctccggc
     3121 tcacgataca aacgagacga tttcaaaata gttaaaacaa acttttcaac cgaaccgtgc
     3181 agacgaagaa cgtctcttaa ctctgatgca agtgccttca tctccagttc ctcgtcaagc
     3241 cagtcgcaaa gctgcccgtt cataatcgta taatcgatca aatataaatt attacacaga
     3301 tagtagcaca attcttctat tgtaaaaata cggcaatgga tgcgcgtaat ctcatacgga
     3361 tgtgccgcat gtctgtcatg acaaagaatt aaatttccca tcattgcctc ctaaatgtat
     3421 cgtcttctcc gcatggaagt ctgtcgccgg gaagaaatct ccaaatccgg catcccttac
     3481 ggaaattctg catgtttttt catcaagaaa cagtgtctca agctgtaaac gcatggaata
     3541 ctccgccctt ttcggaaatc cgtcaagaga cagtgtttcc gttcttacat ttccctgaac
     3601 aagagactca atccgaagct cgatgtcttc tacgtcttcc atgagcactt cccactgatt
     3661 attcgactca taccagtggt tcccccatga aacgatcgga taccacatct cctggcctcc
     3721 aacccgcata ttgaccgtaa tctgatccgt cagtttatct tccgacagat agactggatt
     3781 ttcaatatgc ataaacaact tccggtatgc ggtatagcat gcacccttac tgtaaagatt
     3841 atttccgatg aacgcacgtc tcccattgca caacactcta agtgctttgg gataccagtt
     3901 attttcaaat ccctctcctg tcaaaa
//
LOCUS       DBMDXF010000174         3919 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5396_length_3919_cov_1.637507, whole genome shotgun sequence.
ACCESSION   DBMDXF010000174 DBMDXF010000000
VERSION     DBMDXF010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5396_length_3919_cov_1.637507"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..293
                     /locus_tag="ACZPD4_07040"
     tRNA            209..293
                     /locus_tag="ACZPD4_07040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:aag)
     gene            complement(409..630)
                     /locus_tag="ACZPD4_07045"
     CDS             complement(409..630)
                     /locus_tag="ACZPD4_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378849.1"
                     /translation="MKKEENAVGENIRRLRMRNQLKQADLAQKLNISRQSLSAYERGI
                     TLPDIYLLIEIADFFRISLDELTGRITIH"
     gene            805..1161
                     /locus_tag="ACZPD4_07050"
     CDS             805..1161
                     /locus_tag="ACZPD4_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378850.1"
                     /translation="MLENRVKELRTERGLRQGDLAEKMNVSQQTISRIENGENVLPSD
                     ILIHLSKYFHVSTDYILKLSDVRMTQEYRLEMEQMLLRHFEFFLSYCRLNRTNQKVIS
                     FLAAQMEKAEEKKIKE"
     gene            1382..2917
                     /gene="pap"
                     /locus_tag="ACZPD4_07055"
     CDS             1382..2917
                     /gene="pap"
                     /locus_tag="ACZPD4_07055"
                     /EC_number="2.7.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HIR1378851.1"
                     /translation="MLEKIDLNKKMSKQEYSESLPKIEAELGRLQRECKDLDIPVMIV
                     FEGFGASGKGLQIGKLIHSMDPRGFEVHTIKNETEEERMHPFLWRFWIKTPEKGKIAV
                     FDGSWYRKVWVDRFEKRTREKELKDDFASINAFEQQLSEGGTLIIKLFLDIDQDEQEK
                     RFKKLEKKKETQWRVTQGDKERNVKYDEYALMAEEMLFCTDTDYAPWTIIEATDRRFA
                     TTKIYMTVIQAMQEQIRRQKENSALLEKTNKIVEEVCEEKEVQIAQYAENRFSEVSIL
                     SKTDLSCSYTKEEYKQKLDKLQRKAERLHGELYRKRIPVVIGFEGWDAAGKGGAIKRL
                     TEKMDPRGYVVNPTASPNEVEKAHHYLWRFWKAMPKDGHVAIFDRTWYGRVMVERIEG
                     FCTEEEWKRAYKEINDMEKDLANAGAVILKFWMHIDKEEQKRRFCERMENPEKRWKIT
                     DEDLRNREKWDLYEHAADEMIMRTSTAYAPWVIVEGNDKYYARIKVLKTVTDAIEKRL
                     KER"
     gene            2922..>3919
                     /locus_tag="ACZPD4_07060"
     CDS             2922..>3919
                     /locus_tag="ACZPD4_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1378852.1"
                     /translation="MTIREQLELRELEYLSSYAALSKDSKGRKRKEEPCDIRPAFQRD
                     RDRILHCKAFRRLKQKTQVFLLPKGDHYRTRLTHTLEVSQNARTIAKALRLNEDLAEA
                     IALGHDLGHTPFGHAGERALNHVYHFAHQKQSLRVVERIEKEGRGLNLTWEVRDGILN
                     HQTSGHPHTLEGQVVRISDKLAYINHDMDDAIRGGILTEDDIPSDLRQALGSSCKERL
                     NTLVHDVITNSMDQPVIKMSSETERAMKDLRKFMFENVYLNPNAKGEEDKAVHMIEQL
                     FEYYAEHTEVLPRIFKEALENSDDEKEQIICDYIAGMTDSYAVKKFQDYFVPEAWK"
BASE COUNT         1308 a          563 c         1009 g         1039 t
ORIGIN      
        1 gccggaaaac ttataaatat agtttcagct cggtgtttga aaattgaaaa atgcagatgt
       61 aaaaatcagt ttgaattgtc aatgtgatta aggaggcttt tcacaagatt tttacatctg
      121 attaaaaaaa gtgttgactt tttttgctga taatgatatt atatcattcg tggtcagcaa
      181 aacaaagatc acgaatccta tatggaatgc ggaagtgtcg gaactggcag acgagcaaga
      241 ctaaggatct tgtaagcatt gcgcttgtgt gggttcaagt cccatcttcc gcattagata
      301 aagagaacag gaaatcttag aaaggttttc tgtttttttg cgtggtcttt ttgtttttat
      361 gactaggctc ttcaatttga atgatttctt aaaaagcttc aaattgtttc aatggatggt
      421 aattcttccg gtaagctcat ccagagagat tctgaaaaaa tcggcaatct ctatgagcag
      481 gtagatatcg ggcagtgtga ttccacgctc ataagcggaa agtgattggc ggctgatatt
      541 aagtttttgc gcaagatctg cttgttttaa ctgatttctc atacgcagtc ggcggatatt
      601 ttcgcctacg gcattttctt cttttttcat tgcaaggagg atagatcctg ttttgaaaaa
      661 cacacaatat atattgattt tatcatgctt tttcatcaga gaaatgatat atcctaaata
      721 tgagtaaagg catatccggt attcggatag cacgataaga agtatcggat acaatggaga
      781 aaaacgataa ggggatgtat agaagtgcta gagaacaggg tgaaagagtt gcgtactgaa
      841 cggggattga ggcagggaga tttggccgaa aagatgaatg tttcacagca gacgatcagc
      901 cgtatagaaa atggggaaaa tgttctgcca tcggatatat tgattcattt atcaaaatat
      961 tttcatgtgt caacggatta catattaaag ctatcggacg tgagaatgac acaggaatat
     1021 cgtttggaaa tggaacagat gttattaaga cactttgaat tctttttgag ttactgcagg
     1081 ttgaacagga cgaatcaaaa ggtgatcagc tttttggctg cgcagatgga aaaggcagag
     1141 gagaaaaaaa ttaaagaatg agtttaagag tatataagac ggtaaaagtt tttaagtatc
     1201 agtctcatgt tggtggatgg gaatcagtaa ggacagaaca gatataaaag ggaaaatctt
     1261 atcagatttt tctttttttt attttaccca aactgtttgt tttatgaaat tatatgatat
     1321 aattacgata atttatttga aattgtcaat aatatctgaa taaatgtaag gaggatcagt
     1381 aatgttagaa aagatagatt tgaataaaaa aatgtcaaag caggaatata gtgaaagtct
     1441 tccgaagata gaggcggaac ttggaagact tcaaagagag tgtaaagatt tagatattcc
     1501 ggtcatgatc gtatttgaag gattcggagc atccgggaaa ggacttcaga taggaaagtt
     1561 gatccacagt atggatccgc ggggatttga ggtgcatacg attaaaaatg agacggaaga
     1621 agaacggatg catccgtttt tgtggaggtt ttggatcaag actccggaaa agggaaaaat
     1681 tgcggttttt gatggaagct ggtatcgaaa agtgtgggta gaccgatttg aaaaaaggac
     1741 gcgggagaaa gaactgaaag atgattttgc gtcgatcaat gcttttgaac agcagctttc
     1801 agaaggggga acgctgatta taaaactgtt tttggatatt gatcaggatg agcaggagaa
     1861 acgttttaaa aaattggaga aaaagaaaga gacgcaatgg agagtgactc agggagacaa
     1921 agagagaaat gttaaatatg acgagtatgc gttgatggca gaagaaatgc tgttttgtac
     1981 ggatacggat tatgcgccgt ggacaattat agaagcgact gacaggcggt ttgcgacaac
     2041 aaaaatatat atgacggtga tacaagctat gcaagagcag atccgacgtc aaaaagagaa
     2101 tagtgctctg ttggagaaaa caaataaaat agtggaagaa gtttgtgagg aaaaggaagt
     2161 tcagatcgca caatatgcag aaaacagatt ttcggaagtt tcaatcttgt caaaaacaga
     2221 tctttcctgc agctatacaa aagaagaata taagcaaaaa cttgataaac ttcagaggaa
     2281 ggcggagcgg cttcacggag aattatatag gaagagaatt ccggtagtga tcggatttga
     2341 aggttgggat gccgcaggaa agggaggtgc gattaagcgt ctgacagaaa aaatggatcc
     2401 tcggggatat gtggtaaatc cgacagcttc tccgaatgag gttgaaaaag cgcatcatta
     2461 cttgtggaga ttttggaagg cgatgccaaa agacggacat gtggcgattt ttgacaggac
     2521 atggtatggt cgggtgatgg tagaaaggat cgagggattt tgtacggaag aagagtggaa
     2581 acgcgcatac aaagagataa atgatatgga gaaagatctg gcaaatgcag gcgcggttat
     2641 tctgaaattt tggatgcata ttgataaaga agagcagaaa cgcagatttt gcgaaaggat
     2701 ggaaaatccg gaaaaacgat ggaagattac ggatgaagac ttaagaaacc gtgaaaaatg
     2761 ggatctctac gagcatgcgg cagatgagat gatcatgagg acgtctacgg cctatgcgcc
     2821 gtgggttatt gtggagggaa acgataagta ttatgcacgg atcaaagtgc ttaagacagt
     2881 gacagatgcg attgaaaaga gattaaagga aaggtgatat tatgaccatc agggagcagc
     2941 ttgaacttcg tgaacttgaa tatttaagtt cttatgcggc attaagcaaa gactcgaagg
     3001 gacggaaaag aaaagaggaa ccgtgtgata tccgaccggc ttttcaaaga gatcgtgacc
     3061 ggattcttca ttgtaaagct ttccgaaggc tgaaacaaaa gacgcaggtt tttcttcttc
     3121 cgaaaggaga tcattataga acgcggctta cacatacgtt ggaagtgtcc cagaatgcgc
     3181 gtacgatagc aaaagcattg cgtttgaatg aagaccttgc agaagcaatc gctctggggc
     3241 atgaccttgg tcatacgcct ttcggtcatg cgggggaaag agctttaaat catgtatatc
     3301 atttcgcgca tcaaaagcag agtcttcgtg ttgtagaacg gattgagaaa gaagggcgcg
     3361 gactgaacct gacatgggaa gtccgtgacg gaatactgaa ccatcagacg tcaggacatc
     3421 ctcatacttt ggaagggcag gttgtccgca tttccgataa gcttgcatat atcaaccacg
     3481 atatggatga tgcaataaga ggaggaattc tgacagagga tgatattcct tctgatttgc
     3541 ggcaggctct tggaagttcg tgtaaagagc ggcttaatac tctggtgcat gatgtgatca
     3601 cgaacagtat ggatcagccg gttattaaaa tgtcttcgga aacggaacgg gcgatgaaag
     3661 atctgcggaa atttatgttt gaaaatgtat acttgaatcc gaacgcaaaa ggggaagaag
     3721 ataaggcggt acatatgatc gaacagcttt ttgaatatta tgccgaacat acagaagtcc
     3781 ttccccggat ttttaaggag gctttggaaa attcggatga tgagaaagaa cagatcatct
     3841 gtgattacat tgccggaatg acagattctt atgcggttaa aaaattccag gattattttg
     3901 tgcccgaagc atggaaaat
//
LOCUS       DBMDXF010000175         3857 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5498_length_3857_cov_1.690362, whole genome shotgun sequence.
ACCESSION   DBMDXF010000175 DBMDXF010000000
VERSION     DBMDXF010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5498_length_3857_cov_1.690362"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            309..1283
                     /locus_tag="ACZPD4_07065"
     CDS             309..1283
                     /locus_tag="ACZPD4_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1378853.1"
                     /translation="MNIKGLDVSEFQGEINWERVKNAGYQFAMIRAGYGFNTIDKQFQ
                     RNASECNRIGLPIGVYWFCYAVSPETAIQEADGCLRTIRNYRIDYPVCYDIEQASAAY
                     ALGEGVTITPALAKQLVSSFCNRIEAGGYYAMFYTNRSFLDNYLGNDLAKRYAFWYAR
                     YASSFDGTDCGMWQYTNEGSVPGIGGNVDLDIGYIDYPGIIRRAGLNHLSETAPPPAP
                     PSAPTYITYTIQPGDTLSEIAERFGTTVSSLSALNGISDPNLIYAGNTLRIPQGGNSA
                     PVYYTIQPGDTLSEIAERFGTTVASLSALNGISDPNLIYAGNTLLISQ"
     gene            complement(1647..2066)
                     /locus_tag="ACZPD4_07070"
     CDS             complement(1647..2066)
                     /locus_tag="ACZPD4_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HIR1378854.1"
                     /translation="MKRIINTAIVYFIFAMAGGVFYREFTKWNGYTEPTTLGVLHVHL
                     LVMGTLLFLITALFAKVTMLAENLLFKKFFVLYNIALPAMVVMMLIRGIVQVLGIELG
                     KMGNGMLSGLAGLSHIGMMIALFLLLFAIKRELIRKQ"
     gene            complement(2160..2579)
                     /locus_tag="ACZPD4_07075"
     CDS             complement(2160..2579)
                     /locus_tag="ACZPD4_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378855.1"
                     /translation="MKIKKQTLLLLASLVWMIAGFNVLRIGIETYAEYQTVINYIVTI
                     IVFLLFWFMVFYKLTNKHTIRIQGYEEEKQLFYKFFDLKSFLIMAFMISFGIIIRSFH
                     LLPERFIAVFYTGLGAALFMAGVLFGRNYIQAYRKKA"
     gene            complement(2612..3169)
                     /locus_tag="ACZPD4_07080"
     CDS             complement(2612..3169)
                     /locus_tag="ACZPD4_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1378856.1"
                     /translation="MSRAVTSEEEILKVSCGIVAKKGIAAVNMRTVASECGIALGSLY
                     NYFSSKSELLSATIEAVWKDIFQMGKTLEKCENIVEYLNLLFEGVENSRSRYPKFFSM
                     HALGFTAGERQEGKKAMEVYFLRLKNQMAHFLENDEKIRQGIFTENLTAEIFTDYIFT
                     LFLSILLEKQNEKESFLEFVKNYLY"
     gene            complement(3412..>3857)
                     /locus_tag="ACZPD4_07085"
     CDS             complement(3412..>3857)
                     /locus_tag="ACZPD4_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1378857.1"
                     /translation="PPQPEDKVVIAVEKLADLTVKFGTPAEKLALPKEVKVTLDNKAK
                     DKLSIVWNTDAYKADKAGTYKLTGTLQTKEGITIPEEFRKVTVNVTVEEKGKVPPTSG
                     KEEGKNNSGAVQTGDDKNVWLPIAGLVAAIAVVAAVLIIKNKKKK"
BASE COUNT         1090 a          878 c          583 g         1306 t
ORIGIN      
        1 aaaataagcc agaccatttt gatcggagga gaggcaggaa aagcctgtca cttattatag
       61 gactttattt ttatttttgc aacgcctata aatgaatatt tacctctgct tcttttattt
      121 atctatttta tacattttat tgcaaaaatc aggggacgca cacacatttt cttcggcaat
      181 acatacaata atcatagaaa aaacgagccg taatgcccct ctatacgcgc ttgctttttc
      241 aatatttaat ttgtttttca caaaagaaac taaaatattt acatcaatat attatttatg
      301 gaggcattat gaatataaaa ggacttgatg tcagcgaatt tcaaggagaa atcaactggg
      361 aacgggttaa aaacgcaggt tatcagtttg ctatgattcg tgcaggttat ggattcaata
      421 cgattgacaa acaatttcag agaaatgctt ctgaatgcaa cagaatcggt cttccgatcg
      481 gagtttactg gttctgctat gctgtcagtc ctgaaacggc tattcaggaa gccgacggct
      541 gtctgcgcac cattcgtaat tatagaattg attatcctgt ctgctatgat attgaacagg
      601 cgtccgccgc ttatgctctc ggagaaggtg tcactatcac tccggctctt gcaaaacaac
      661 tcgtttcttc tttctgcaat cgtattgaag cagggggcta ctatgcgatg ttttacacaa
      721 accgcagttt tttagacaat tatctcggaa atgatcttgc aaaacgatat gcattctggt
      781 acgcccgcta tgcgtcttcc ttcgacggaa cagactgcgg tatgtggcaa tatacaaacg
      841 aaggaagtgt tcccggtatc ggcggaaatg tagaccttga tatcggctac atcgattatc
      901 ccggcatcat ccgccgggca ggacttaatc acctgtctga aactgctccg ccgcccgctc
      961 ctccctccgc tccgacatat atcacttata cgattcaacc cggtgataca ttaagtgaaa
     1021 ttgccgaaag attcggaaca accgtctctt ctctttccgc tctgaacggt atttccgatc
     1081 ccaatcttat ttacgccgga aatacactcc gcattccaca aggcgggaat tctgctccgg
     1141 tttattacac cattcagccg ggagacacat taagcgaaat tgccgaaaga ttcggaacga
     1201 ccgtcgcttc tctttctgcc ttgaacggta tttctgatcc caacttaatt tatgcgggaa
     1261 atacacttct aatttctcaa taactttcag taccgacaga tttttttatc ttttcttttc
     1321 aataaagcgc ggtttcttta actatctaaa tttaagaatt tcataacttt ttttcgtttt
     1381 cttttagaca ttaaacatac tttagacaca ttttgaagat atactgttca acagatagtt
     1441 gataaaacaa ctatctcccc cctcataaag taaagacatc ccggataacc gggatgtcgc
     1501 actttactct cattccttta gataactata aaaacaacga aacccgttag ccggaaggtt
     1561 agcgggtttt cgctgtttat agaactgttc atctcgtctg tggatagttc ttagacaatt
     1621 cactataact ctaaaaatca ttattcctat tgttttcgta tcaattccct ttttatcgca
     1681 aacagcagta agaaaagtgc aatcatcatt ccgatatgag acaatcctgc cagaccggac
     1741 agcatcccat tccccatttt tccaagctct attccgagaa cctgcacaat tccgcgaata
     1801 agcatcatca ccaccatggc aggcaatgcg atattataga gaacaaaaaa tttcttaaac
     1861 aaaagatttt ccgccaacat tgttactttt gcgaaaagag ccgttataag aaataacaat
     1921 gttcccatca ccaacagatg aacatgcagc actcctaatg tagtcggctc tgtatatccg
     1981 ttccattttg taaattcccg ataaaatacg cctcccgcca ttgcaaatat aaaataaaca
     2041 atcgctgtat ttataattct tttcatatgt atcttctcct tagtttttac tcctcatctt
     2101 ttatgcatct atactttctc tatacttcct ctatactttc tctatgcttt ttcgtttact
     2161 tatgctttct tcctgtaagc ctgtatataa tttcttccaa ataaaacgcc tgccatgaaa
     2221 agtgccgctc ccaatcctgt gtaaaataca gctataaacc gctccggcag taaatggaat
     2281 gaacgaataa taatcccgaa acttatcata aacgccataa ttaaaaatga tttcaaatca
     2341 aaaaatttat aaaacagttg cttttcttct tcatatcctt gaattcttat tgtatgttta
     2401 tttgtcaact tataaaatac catgaaccaa aagagaagaa acacgataat tgtcacgata
     2461 taatttatta cagtctgata ttcagcataa gtttcaatcc cgatcctcaa aacgttaaat
     2521 cctgcgatca tccagacaag acttgccaaa agcaaaagag tctgtttctt tatcttcata
     2581 acccatctcc gttccacatc aactctttct cttaatacaa ataatttttt acaaattcca
     2641 aaaaggactc tttttcattt tgtttttcca gtaatattga taaaaacaag gtaaatatgt
     2701 agtccgtaaa aatttcggca gtcagatttt ctgtaaatat cccttgacgt attttttcat
     2761 cattttccaa aaaatgtgcc atttgatttt ttaacctcag aaaatatact tccatcgcct
     2821 tttttccttc ttgtctttct cctgccgtaa accccaatgc atgcatggaa aaaaatttcg
     2881 gataacggct tcggctattt tcaacacctt caaacaacag gtttaagtat tccacaatat
     2941 tctcgcattt ttctaaagtt tttcccattt gaaatatatc tttccacact gcctctattg
     3001 tagcagacaa aagttctgat tttgaagaaa aataattata caaagaacct agtgcaattc
     3061 cgcattcaga tgcaacggtt ctcatattta ctgcggcaat tccttttttt gccacaatcc
     3121 cacagcttac tttcaatatt tcttcttccg acgtaaccgc tctgctcaac tcttatcacc
     3181 gtcctcttta ttctattctc ctttcccgct ttcccttttc agttttttta atatgaacat
     3241 tgttcatgtt aatattctat acaatatttg tcaagttttt tctcctgttt tccaattttt
     3301 ctctttactc tctttttaca acttcaaaag aaccgattcc caaaagagaa tcggttcttt
     3361 tatatcgcct cacaatatta tattatgaaa actttattct tatttttgtt cttatttttt
     3421 ctttttattt ttgataataa gaactgccgc cacaacggcg attgcagcca caagacccgc
     3481 gatcggaagc catacgttct tatcgtcgcc tgtctgtacc gcaccggaat tgttctttcc
     3541 ttcctctttg ccggatgtcg gaggaacttt tcctttctct tcaacagtca cgttgaccgt
     3601 tactttcctg aattcttccg gaattgtgat tccttctttt gtctgaagtg ttcctgtcag
     3661 tttatatgtt cctgctttgt ctgctttata cgcatctgtg ttccatacga ttgacagttt
     3721 atctttcgcc ttgttatcaa gtgtcacttt cacttctttc ggaagggcaa gtttctctgc
     3781 cggagttccg aatttcacag taaggtcagc aagtttttct actgctataa ccactttgtc
     3841 ttccggctgc ggcgggt
//
LOCUS       DBMDXF010000176         3831 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5539_length_3831_cov_1.716354, whole genome shotgun sequence.
ACCESSION   DBMDXF010000176 DBMDXF010000000
VERSION     DBMDXF010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5539_length_3831_cov_1.716354"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..514)
                     /locus_tag="ACZPD4_07090"
                     /pseudo
     CDS             complement(1..514)
                     /locus_tag="ACZPD4_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019851.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     assembly_gap    515..524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(592..1494)
                     /gene="argB"
                     /locus_tag="ACZPD4_07095"
     CDS             complement(592..1494)
                     /gene="argB"
                     /locus_tag="ACZPD4_07095"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1378858.1"
                     /translation="MNKNMQQYLDKAEVLIEALPYIQRFNRKIIVVKYGGSAMIDEEL
                     KKRVIEDVTLLKLVGFKPIIVHGGGKEISKWVEKAGMEPKFINGLRVTDKDTMELAEM
                     VLGKVNKSLVQLVESLGVRSIGISGKDGALLKVAKKYSDGQDIGYVGEVTEVNEQIIY
                     DLLEKDFLPIICPVGMDENYHTYNINADDAACAIARAVKAEKLAFLTDIEGVYKDPED
                     PSTLISELCVKEAEKLMTEGYIGGGMLPKLQNCIDAIEAGVSRVHILDGRIPHCLLLE
                     IFTNKGIGTAILNSTESRYNHDEQ"
     gene            complement(1563..2786)
                     /gene="argJ"
                     /locus_tag="ACZPD4_07100"
     CDS             complement(1563..2786)
                     /gene="argJ"
                     /locus_tag="ACZPD4_07100"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1378859.1"
                     /translation="MKVIDGGVTAAKGFQAAAAAAGIKYKDRTDMALIYSEKPCKAAG
                     TFTTNLVKAAPVKWDRAIVESKRKPQAVIVNSGIANACTGEEGMKYCEETAKEAACVL
                     GVEAESVLVGSTGVIGMQLPMDRVKSGIKMLAEAKNSSKEKGTEAAKAIMTTDTKKKE
                     AAVTFEIGGKTVTIGGMSKGSGMIHPNMCTMLAFLTTDAAITKKALQCALSEDVEDTY
                     NMISVDGDTSTNDTVLLLANGMADNKKIKYGTKEYEQFKKALHYVNETLAKEMAGDGE
                     GATALFEVKVVGAKTKEQAKVLSKSIVCSNLTKTAIAGHDANWGRILCAMGYSGAQFD
                     PEKVDLFFESAAGKLQIIENGVALNYSEEKATEILSEPKVTAIADLKEGDAAATAWGC
                     DLTHEYISINADYRS"
     gene            complement(3126..>3831)
                     /locus_tag="ACZPD4_07105"
     CDS             complement(3126..>3831)
                     /locus_tag="ACZPD4_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1378860.1"
                     /translation="MKRVRKQRKLFIFGLILLLIGCGILLYPHILQIFYKIKTESMFR
                     QFEETTENVRDANPEKYARLYEEMKNYNEELIKNNQENLADPYAYANPAIRLEEYGVE
                     NGIAGYIKIPVMDLELPLYLGANEENMSKGAAQLTETSMPIGGNNTNMAVVAHRGYSY
                     AKMFREIEKLKIGDEIFITNFWETMTYKVERIEVISPDEIDKIRIQEGKDMVTLVTCH
                     PYPHNYQRYLVYCERV"
BASE COUNT          931 a         1011 c          608 g         1271 t
ORIGIN      
        1 ataaatgccg ccctctccct gaagcggttc taaaataatc gcacacgttt tctcattcac
       61 aagcgacttc acactgtcaa gatcgttaaa ttttgcaaac tttacccccg gaacaagcgg
      121 ctcaaaaggt tctctgtaag aatcatgtcc tgtaaccgat accgctccaa atgatctgcc
      181 gtgaaacgag ttttccatag cgataaattc atatcgtccc gatccttttg tatatgcata
      241 tcgtctcgct gcttttaaag ccccttcatt tgcttcgccg ccgctgtttg taaaaaatac
      301 ccgatccatt ccgctgactt tattcagcgc ttccgccgct tccccgcagc tttcatgata
      361 ataaaggttg gacgtatggt ataatttatc aatttgtttt ttcagcgcct cgtttaattc
      421 tttattgtca tacccaagcc ctgtcactgc gaagcctgcc gcaaaatcaa gatattcttt
      481 tccgtctgta tcatacagat acattccctt tccannnnnn nnnntccatg gtcaagtgcg
      541 atcggaaaac gattatatac atgaataagg ttttcctctg ccgcctgtat cttactgttc
      601 atcgtgatta tacctgcttt ctgtactatt taaaatcgct gttccgatcc ctttattagt
      661 gaaaatctca agaagaagac aatgcggaat ccttccgtca agaatatgca ctcttgaaac
      721 acctgcttcg atcgcatcga tacagttctg aagtttcgga agcattcccc ctccgatata
      781 accttctgtc attagttttt ccgcctcttt tacacaaagt tccgagatta aggtagacgg
      841 atcttccgga tctttataca cgccctcgat atcagtcaaa aacgctaatt tttccgcttt
      901 caccgcgcgc gcgatcgcac acgccgcatc atctgcatta atattatatg tatggtaatt
      961 ttcatccata ccgaccggac aaatgatggg aaggaaatct ttttccagga gatcgtaaat
     1021 aatctgttca tttacctctg taacttctcc tacatatccg atatcctgac cgtcagagta
     1081 tttcttcgcc acctttaaca aagctccgtc cttaccgctg ataccaatcg acctcacgcc
     1141 aaggctctca acaagttgaa caagactttt atttactttt ccaagtacca tctcggctaa
     1201 ttccatcgta tctttgtctg tcacacgaag cccattgata aacttcggtt ccataccggc
     1261 tttttcaacc catttgctaa tctcttttcc gccgccatgt acgatgatcg gcttaaatcc
     1321 gacaagcttc aaaagcgtca catcctcaat aacgcgcttt ttcaattctt cgtcgatcat
     1381 agcgcttccg ccgtatttca caacgatgat ctttcggttg aaccgctgga tatacggcaa
     1441 agcttcaatc aaaacttctg ctttatcaag atattgctgc atgtttttat tcatgttctc
     1501 tctccaatct ttttgtctcg tatctttgat cctgcaaata actctttttt cactgacatg
     1561 ttctagcttc tgtaatctgc attaatgctg atatattcat gagtcagatc gcatccccat
     1621 gccgttgccg cggcatcacc ttctttcaga tcggcgatcg cagtcacctt aggctcagat
     1681 aaaatttccg ttgctttttc ctcactgtaa tttagcgcaa caccgttttc aatgatctga
     1741 agctttcctg ccgcactttc aaaaaacaaa tccaccttct caggatcaaa ctgtgctccc
     1801 gaatatccca tcgcacagag gattctgccc cagttcgcat cgtgacctgc tattgccgtt
     1861 ttcgtcaaat ttgagcagac gatcgatttc gacaaaacct ttgcctgctc tttcgtcttc
     1921 gcgcctacga cttttacttc aaaaagcgcc gtcgcgcctt ctccgtctcc cgccatctct
     1981 tttgcaagcg tttcattgac ataatggaga gctttcttaa actgttcgta ttcttttgtt
     2041 ccatatttaa tcttcttatt atctgccatt ccgttagcca gcaaaagaac cgtatcgttt
     2101 gtagatgtat ctccgtctac ggaaatcatg ttgtaagtat cttccacatc ttcacttaac
     2161 gcacactgaa gcgctttttt tgtaattgcg gcatccgttg tcagaaaggc taacattgta
     2221 cacatatttg gatgaatcat tcccgatccc tttgacatcc cgccgatcgt cacggtcttc
     2281 cctccgatct caaaggtaac tgccgcttct ttcttctttg tatccgttgt catgatcgct
     2341 tttgctgcct ctgttccctt ttctttgctg ctgttctttg cctctgccag cattttgatt
     2401 cccgatttca cccggtccat cggaagctgc ataccaatca ctcctgtgga accgacaagc
     2461 acgctttccg cctctacgcc aagaacacaa gccgcctctt ttgccgtttc ttcacaatat
     2521 ttcattccct cttctccggt acatgcattt gcaatgccgg aatttacgat cactgcctgt
     2581 ggtttccgct ttgactccac gatcgcgcga tcccacttta caggagccgc tttcacaagg
     2641 tttgttgtaa aagttcccgc cgccttacac ggcttttcac tgtagatcag cgccatatcg
     2701 gtacggtctt tatacttgat gcccgctgcc gctgccgctg cctgaaatcc ttttgccgct
     2761 gttactccgc catcgatcac tttcattttg tcctctcctt tttgtaccct atatgttctg
     2821 tcaaagtttc ttctataaaa gaaaactccg cctcttaaaa gaggcgaagt caatccgcga
     2881 tttcacttta atgaaagaag aaccgtttct ttatcaaaac gtctgtattt ctgttttttg
     2941 ttctcccccc gcctttttct gtgtcgacga ttgttttaaa tcatctaacc gtctaaattc
     3001 tatacgcaaa tattattagc tattatatta tgaaataaaa tctgttttgt caactatttc
     3061 atcttttgaa ttacgggcta tacgaatcat ttttaaatta ccggacatgg attttataga
     3121 tggattcaaa ctctctcaca ataaactaaa taccgctgat aattgtgagg atatggatga
     3181 caagtcacaa gagtcaccat atctttgcct tcctgtattc tgatcttatc aatctcatcc
     3241 ggagatatca cctctatccg ttccacttta taagtcatcg tttcccagaa gtttgtaata
     3301 aatatttcat ccccgatttt caatttttca atctcacgaa acatctttgc ataactgtat
     3361 ccccgatgtg cgacaaccgc catattcgta ttattcccac cgatcggcat cgacgtttcc
     3421 gtaagctgag cagctccttt tgacatattt tcttcgttag cgcccaaata taacggaagc
     3481 tcaagatcca taaccggaat tttaatatat cctgcaattc cattttctac tccgtactct
     3541 tccaaacgga ttgccgggtt tgcatatgca tacggatctg ccagattttc ctgattgttt
     3601 tttataagtt cttcgttata gttcttcatt tcttcataaa gccgggcata tttttcagga
     3661 ttcgcatccc ggacattctc cgtcgtttct tcaaactgac gaaacatact ctctgtcttt
     3721 atcttataaa atatctgaag aatatgcgga taaagcagaa tcccgcatcc aatcaaaaga
     3781 agaatcagac cgaaaataaa aagttttctt tgttttctca ctcttttcat c
//
LOCUS       DBMDXF010000177         3788 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5600_length_3788_cov_2.071603, whole genome shotgun sequence.
ACCESSION   DBMDXF010000177 DBMDXF010000000
VERSION     DBMDXF010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5600_length_3788_cov_2.071603"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /gene="carB"
                     /locus_tag="ACZPD4_07110"
     CDS             <1..2134
                     /gene="carB"
                     /locus_tag="ACZPD4_07110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1378861.1"
                     /translation="VVTKMPRFPFDKFADANQKLGTQMKATGEVMSIGRTFEESLLKA
                     IRSLETGVFHLYMEKFDEKTREELLSYIKAGTDDRIFAVAQLLRLGCNVQQIAFETKI
                     DGFFLEKMKKIIEMENMLKANPWNRDVLHEAKRGGFSDRAVAWLWNTDEDTVFRIRKE
                     MHLFPVYKMIDTCASEFDSYVPYFYSTYEEENESIAEDRKKILVLGSGPIRIGQGVEF
                     DYSTVHAVKTIRNAGYEAIVINNNPETVSTDYTTSDKLYFEPLCVEDVMNVIELERPD
                     GVIASLGGQTAVNLAEPLESRGVTIIGTDCEAIEKAENRESFEKLLAALSIPQPKGQA
                     VTNIEDGVKAAAAIGYPVLVRPSFVLGGRAMQIVAKEEQLRQYLKTAVEINEEKPVLV
                     DHYIRGKEVEIDGICDGQDVFVPGIMELVERTGVHSGDSISVYPSYSISDHVKEVILD
                     YTRRLGLGIGIRGLFNIQFIVDQEENVYIIEVNPRSSRTVPFLSKATGYSLADIATRV
                     ILGISLKEQGIDTCYPEEKSFWYVKAPAFSFAKLNGMDAYLSPEMKSTGEAIGYDRKF
                     PRAMYKALIASGVKMQNYGTVMVTLADEDKEEALPLIRRFYEMGFNIEATVGTGEFLK
                     AHGIRTRIRRKLSENSTEILEAIRSGYVSYVINTRAILSGVHYEDGLAIRRTAIENNV
                     TMFTSLDTVKVLLDVLEEITIGVSPLYA"
     gene            2159..3349
                     /locus_tag="ACZPD4_07115"
     CDS             2159..3349
                     /locus_tag="ACZPD4_07115"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR1378862.1"
                     /translation="MIPNMNYQNLKESYLFYNIAQKTKAYLEAHPGAHLYRMGIGDVS
                     LPLCDAVIQKLHEAVEDQAHKTTFHGYMPECGDTELRTTIAAYYQKRGVKLSHEEVFV
                     SSGASDELGDILDLFGKEKTVLIMEPAYPAYVDANVIAGNTIIHIPAGEENGFVPVPD
                     PDIAADVIYICSPNNPTGAVFDREALQAWVDYANKMNAIILFDAAYEAFIEEDDIPHS
                     IFEIPEARTCAIEICSLSKTAGFTGTRCGYTVIPKELFRGGMSLNQMWVRNRTTKTNG
                     VSYLIQKGASAVFTEEGQRQIREGIQIYKKNGRCLTEVLDRLGIWYCGGKNAPYIWMK
                     CPNGMGSWEFFDYLLNEIQVVGTPGEGFGACGEGYFRFSTFGSPEDTKEAAGRLELLL
                     QKLL"
     gene            3562..3788
                     /gene="pstB"
                     /locus_tag="ACZPD4_07120"
                     /pseudo
     CDS             3562..3788
                     /gene="pstB"
                     /locus_tag="ACZPD4_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
BASE COUNT         1165 a          710 c          962 g          951 t
ORIGIN      
        1 tgtagtaacg aaaatgcctc gttttccgtt tgataagttt gcagatgcca atcaaaagtt
       61 aggcacgcag atgaaagcga ccggagaggt gatgagcatt ggacgaacat ttgaagaaag
      121 tctgttaaag gcgatccgtt ccctggaaac gggagtattt catctatata tggagaaatt
      181 cgatgaaaag acaagggaag agcttctttc ctatataaaa gcaggaacgg acgacaggat
      241 ctttgccgtt gcacaattat taagacttgg ctgcaatgtt cagcaaattg cttttgaaac
      301 aaagattgac ggctttttcc tggaaaaaat gaagaaaatc atagaaatgg aaaatatgtt
      361 aaaagcaaat ccatggaaca gggacgtcct gcatgaggca aaaagaggag gattttccga
      421 tcgcgctgtg gcatggctct ggaatacaga cgaagatact gtctttcgca ttagaaaaga
      481 aatgcatctg ttcccggttt ataaaatgat cgacacctgc gcttctgaat ttgacagtta
      541 tgtcccttat ttttattcta cttacgaaga agaaaacgaa tcgatagcag aggacagaaa
      601 aaaaattctg gtgcttggat caggtcctat ccgaatcggt cagggcgtag agtttgatta
      661 ttcgacagtt catgccgtaa aaacaatacg caatgccgga tatgaggcca ttgtgatcaa
      721 caataatccg gaaacagttt ctacggatta tacgacttcc gataagctgt attttgaacc
      781 tctctgtgtc gaagacgtga tgaacgtaat tgaactggag cggccggatg gcgtaatcgc
      841 ctcgctggga gggcagactg ccgtgaatct ggcagagcct ctggaatcca gaggcgtaac
      901 gatcatcgga acagactgcg aggcgatcga aaaggcagag aacagggagt cctttgagaa
      961 gcttttggcc gcgctttcta ttccgcagcc caaaggtcag gctgtaacga atattgaaga
     1021 tggtgtaaag gcggcagctg caataggcta tcctgtgctg gtacgtccga gctttgtatt
     1081 aggcggaaga gccatgcaga tcgtggcaaa agaagaacaa ctgcggcaat atctgaaaac
     1141 ggcggtggag atcaatgaag aaaaaccggt attagtggat cattatatca gaggaaaaga
     1201 agtggagatc gacggaatct gcgacggtca ggatgtattt gtgcccggca tcatggaact
     1261 ggtggagaga accggcgtac attccggcga ttccatcagt gtgtatccga gttacagcat
     1321 ttcagaccat gtaaaagaag tgattctgga ttatacaaga agattaggtc ttggaatcgg
     1381 aatcagagga ttgtttaata ttcagtttat tgtggatcag gaggaaaacg tgtatattat
     1441 agaagtaaat ccacgctcat ccagaaccgt tccgttttta tcaaaagcca caggttattc
     1501 tctggcggat attgcgacaa gagtgattct tggcatcagc ctgaaagagc agggcatcga
     1561 cacatgttat ccggaagaaa agagtttctg gtatgtaaaa gcgcccgcgt tttcttttgc
     1621 taagttaaat ggaatggatg cgtatctgtc ccctgagatg aaatcaacag gagaagccat
     1681 aggatacgat cgaaaatttc ctcgagccat gtataaagcc ttaatcgctt ccggtgtgaa
     1741 aatgcagaac tacggaacgg tcatggtaac tctggctgat gaagataaag aagaggcgct
     1801 gcctcttatt cgacggtttt atgaaatggg attcaatata gaggcaacag tcggaaccgg
     1861 agagtttctg aaagcgcatg ggattcgcac tcgtatccgc agaaagctta gtgaaaacag
     1921 caccgaaatt ctggaagcga taagatccgg ctatgtaagc tatgtcatta acacaagagc
     1981 cattttatcc ggcgttcatt atgaagacgg actggcgatc agaaggacag ccatcgaaaa
     2041 caacgtaacg atgtttacct ctttggatac cgtaaaggta cttctggacg tgctggaaga
     2101 aattaccata ggtgtttcgc ctctttacgc ataaaaacaa aaaaaggaga actgcagaat
     2161 gataccaaat atgaattatc aaaatttaaa agaatcttat ttgttttata atatcgccca
     2221 gaaaacaaaa gcttatctgg aagcacatcc gggagcgcat ctctatagaa tgggaattgg
     2281 cgacgtttct ctgccgcttt gcgatgccgt tatccaaaaa cttcatgagg cagtagaaga
     2341 tcaggctcat aaaacaacct ttcatggata tatgccggaa tgcggagata cggaacttcg
     2401 gacaaccatt gcggcatact atcaaaaaag aggagtaaaa ttaagccatg aggaagtctt
     2461 tgtctccagc ggggcaagtg atgagctggg agatattttg gatttgttcg gaaaagaaaa
     2521 gacagtgctg atcatggaac cggcttatcc tgcttatgtg gatgcgaacg tgattgccgg
     2581 aaacacgatt atccatattc cggcgggaga agagaacgga tttgttcctg tgccggatcc
     2641 ggacattgcg gcagacgtga tctacatctg ctctccgaat aatccaaccg gcgctgtatt
     2701 tgaccgggag gcattacagg catgggtgga ttatgccaac aaaatgaatg ccatcatcct
     2761 gtttgatgcc gcatatgagg catttattga agaagatgac atcccccaca gtatttttga
     2821 aatcccggaa gcgagaacct gtgccattga aatttgctct ttgtctaaga ccgcgggctt
     2881 tacaggaaca cgctgcggat atacagtcat tccaaaagaa ctgtttcgcg gcggaatgag
     2941 tctgaaccag atgtgggtaa gaaaccggac gacgaaaaca aacggtgtat cttatcttat
     3001 ccaaaaagga gcttcggctg tatttacgga agaaggacag cggcagattc gggaggggat
     3061 tcaaatctat aaaaagaatg gaagatgtct gacggaagta ctggatcggc tcggcatctg
     3121 gtactgcgga gggaaaaacg ctccttatat ttggatgaaa tgcccgaatg gaatgggaag
     3181 ctgggagttt tttgattacc tgcttaatga aatccaggta gtgggaacgc ccggagaagg
     3241 gtttggagcg tgcggcgagg gatatttccg tttctctact ttcgggtcgc cggaagatac
     3301 gaaagaggcg gctggaaggc tggaactgct tcttcagaaa ctgctttgat ttcgggccgt
     3361 catgaacctg cctgtaaggg agcgcggttt taatcttata aattttactt tgatttaaca
     3421 atgaagcgct agtcgatttt acatgttttt tatacaatga tatcacagcg gtaaaatgta
     3481 atgttgtgaa aatgaagaaa agatgatatg ccgtacatcc ggtatttaca atgagatgag
     3541 gaaattttgc ctaaaacgta ggacgagcgg acatcggctc tcttttcggt cttcatatca
     3601 aagactgaag agcttgcgat ggagctgaaa aaagattata ctattgccat agttacatat
     3661 aatatgcagc aggcagtgcg catgtctgcc aatacggcgt ttttccttct cggagaagtg
     3721 tggaatgcaa caatacagaa aaactgtttt caataccgca aaataaaaag acagaagatt
     3781 acattacg
//
LOCUS       DBMDXF010000178         3746 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5657_length_3746_cov_1.462551, whole genome shotgun sequence.
ACCESSION   DBMDXF010000178 DBMDXF010000000
VERSION     DBMDXF010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_3746_cov_1.462551"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="ACZPD4_07125"
     CDS             <1..230
                     /locus_tag="ACZPD4_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378863.1"
                     /translation="VTALDGKAGIGFVRTTADTAGETRDVLKDLPIPSEIKQIIARKK
                     NRNRIIRFFRSRSFSNIRNRSAIRHRPFYYF"
     gene            complement(255..1241)
                     /gene="gpr"
                     /locus_tag="ACZPD4_07130"
     CDS             complement(255..1241)
                     /gene="gpr"
                     /locus_tag="ACZPD4_07130"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR1378864.1"
                     /translation="MIKNYSVRTDLALEEKERFESDHVEISGVVLEEDYDEASELRVT
                     RVEIKTENGAKAMGKPVGTYLTIETPNLAMPDEKSQTVIAEKVCSFIKELIRKFEVKD
                     KDLSILVVGLGNREVTPDSLGPCVADHLSVTRHIVKEYGKYAIGVDDAPMISAVVPGV
                     MGQTGMESAEIVRGIVSETNPDVIIAVDALAARNSKRLNRTIQIADTGIHPGSGVGNH
                     RNGMTMETLGVPVIGIGVPTVVDAATIVNDTMENFIRALESSDSLKGVGEVLRSYNAG
                     EKYEFVKELISPHLNGMFVTPKDVDEMVHHISHTLSEAINMLFSAGSGRSEA"
     gene            1525..1788
                     /gene="rpsT"
                     /locus_tag="ACZPD4_07135"
     CDS             1525..1788
                     /gene="rpsT"
                     /locus_tag="ACZPD4_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1378865.1"
                     /translation="MANIKSAKKRILVNETKAARNKAIKSKVKTAVKKVEAAVAAKDA
                     ETAKTALRAAIVEISKAGTKGVYHKKTVSRKISRLSKAVSSIA"
     gene            complement(1945..>2608)
                     /gene="ilvC"
                     /locus_tag="ACZPD4_07140"
     CDS             complement(1945..>2608)
                     /gene="ilvC"
                     /locus_tag="ACZPD4_07140"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR1378866.1"
                     /translation="PPKNVDVTMIAPKAPGHTVRSEYQVGKGTPCLVAVEQDYTGKAL
                     DKALAYGLAIGGARAGILETTFRTETETDLFGEQAVLCGGVCALMQAGFETLVEAGYD
                     PRNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIITDDTKKAMKQI
                     LTDIQDGTFAKDFLLDMSEAGGQAHFRAMRKLASEHPAEKVGEEIRKLYSWSDEDKLI
                     NN"
     assembly_gap    2609..2618
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2619..2979)
                     /locus_tag="ACZPD4_07145"
     CDS             complement(<2619..2979)
                     /locus_tag="ACZPD4_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1378867.1"
                     /translation="MAAKIYYQEDCNLSLLEGKTIAIIGYGSQGHAHALNLKESGCNV
                     VIGLYEGSRSWAKAEAQGFEVYTAAEAAKKADIIMILINDEKQAALYKNDIEPNLEEG
                     NMLMFAHGFNIHFGQIVP"
     gene            complement(3003..3500)
                     /gene="ilvN"
                     /locus_tag="ACZPD4_07150"
     CDS             complement(3003..3500)
                     /gene="ilvN"
                     /locus_tag="ACZPD4_07150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1378868.1"
                     /translation="MRSQVFSLLVDNNPGVLSRIAGLFSRRGYSIDSITAGATTDTRF
                     TRITVVASGDELILSQIEKQVRKLEDVVDIKVLKDGESVYRELIMVKLRANASQRGEI
                     ISVADIFRAKIVDVEEDSLIIELTGTQSKLEAFLNLLRGYEILELARTGITGLSRGSK
                     DVAYF"
BASE COUNT          992 a          912 c          620 g         1212 t
ORIGIN      
        1 tcgtcaccgc cctcgatgga aaagcgggca tcggcttcgt ccggacgacg gccgatacgg
       61 caggggagac acgtgacgtt ttaaaagacc ttcccattcc ttccgaaatc aaacaaatca
      121 ttgccagaaa gaaaaacaga aaccggatca tccgcttctt ccgttctcgt tctttttcta
      181 atatccgaaa tcgatccgcc atcagacatc gtcctttcta ttacttctga cagtgtatgc
      241 tttgttcttt tctcttatgc ttccgatctg cctgaaccgg ccgaaaaaag catattgatc
      301 gcttccgata aagtatgact gatatgatga accatttcat caacatcttt aggcgtgaca
      361 aacattccgt ttaaatgagg agagatcagc tcttttacaa attcatattt ttctccggcg
      421 ttataggaac gcagcacttc cccgactcct tttaaagaat ccgaagattc aagagccctt
      481 ataaagtttt ccattgtatc atttacaatc gttgctgcgt ctacaactgt cggcacaccg
      541 attccgataa caggtactcc taatgtttcc attgtcatcc cgtttctgtg attccccact
      601 ccacttccgg gatgaatccc ggtatctgcg atctgaatcg tccggttcaa tcgcttactg
      661 tttcttgctg ccaatgcatc aactgcaata ataacatcag gattcgtttc cgatacaatc
      721 ccacgcacga tctccgcact ttccattccc gtttgcccca tcactcccgg cacgacagcg
      781 ctgatcatcg gcgcgtcgtc gactcctatt gcatattttc catattcttt tacaatatgc
      841 cgcgtcacgg ataaatgatc tgccacgcaa ggtcccagtg aatccggagt aacttctcta
      901 ttccccagcc ctactacaag aatagataga tctttatctt ttacttcaaa ttttcggatc
      961 agttccttta taaaagaaca taccttttcg gcaatcacgg tctgactttt ttcatcaggc
     1021 attgccaaat taggagtttc tatcgtcaaa taagttccta caggttttcc cattgccttt
     1081 gctccgtttt ctgttttgat ctccactctt gtaacacgaa gttctgatgc ttcatcataa
     1141 tcttcttcaa gaactacccc tgatatttct acatgatctg attcaaacct ttctttttct
     1201 tcaagcgcaa gatctgtccg cacactataa ttttttatca tatctcatcc tcgcttttta
     1261 tgttcttatt atagtattcc tgctgttttt atattcaaac cgtcttttta atgcagatac
     1321 tgtgtttttt tattccggtt ttccaaataa tgcagttttt ctctatatat gttccataat
     1381 tcccaatatt tattaaaata tgtattgaca accctttaga gttggcataa aataaatgag
     1441 tatgtttcag cagaacgtcc gtgtccgagt ctgcggaaac tcaaattaca tttgaaatca
     1501 tagatatatt tggaggtgta cgcattggct aacattaagt ctgctaagaa aagaatttta
     1561 gtaaacgaga caaaagctgc aagaaacaaa gcaattaagt ctaaagtaaa aactgctgta
     1621 aagaaagtgg aagctgcagt tgccgctaaa gacgctgaaa ctgctaagac tgctcttcgc
     1681 gcagcgatcg tcgagatttc caaagctgga acaaaaggtg tttatcacaa aaaaactgtt
     1741 tccagaaaga tttctcgtct ttccaaagct gtaagcagca ttgcttaatc ttattaatag
     1801 aattgataaa aatagaaaag acatttgctc ccgtcaggca gatgtctttt tttgtacata
     1861 atcttatcat cataaacaaa aagacatttt catacaaaaa gacgcttcgc gtaatgcaag
     1921 gcgtcttttt gtaattatat cctattagtt attgatcagt ttatcttcat cgctccaact
     1981 gtaaagtttt ctgatctctt ctcctacttt ctctgccgga tgctctgacg caagttttct
     2041 cattgcgcgg aaatgtgcct gacctccggc ttcggacata tccagaagaa agtcttttgc
     2101 aaacgtacca tcctgaatat ctgtcaaaat ctgcttcatc gctttcttcg tatcatctgt
     2161 aatgatcttc ggtcctgtta tgtaatcacc atattcggca gtattggaaa tcgaatatct
     2221 cattcctgca aaaccggact gatagatcaa atcaacaata agcttcatct catgaataca
     2281 ctcaaaatat gcatttctcg gatcgtatcc cgcttctaca agtgtctcaa agcctgcctg
     2341 cataagagcg cacacaccgc cgcaaagaac cgcctgttcg ccgaaaaggt ctgtctctgt
     2401 ctctgtacgg aatgtagtct cgaggatacc ggctcttgct cctccgatcg caagaccata
     2461 tgcgagagct ttatcaagag cttttcctgt ataatcctgc tctacagcta caaggcaagg
     2521 agttcctttt ccaacctgat actcgcttct tacagtatgt cccggagctt tcggagcgat
     2581 cattgtcaca tctacgttct tcggaggtnn nnnnnnnngc ggaacgattt gtccgaaatg
     2641 aatgttaaag ccgtgtgcaa acatcagcat attaccttct tcaagattcg gctcaatatc
     2701 gtttttatat aaagcagcct gtttctcatc attaatcagg atcataataa tatctgcttt
     2761 ttttgccgct tctgccgctg tatatacttc aaatccctgc gcttctgctt ttgcccatga
     2821 tctgcttcct tcataaagtc cgatcacgac attgcatcct gattctttca gattcagtgc
     2881 atgagcatgt ccctgacttc cataaccgat gatcgcgatt gtttttccct caagaagtga
     2941 gagattacaa tcttcctgat aataaatttt tgctgccatt ttgcattcct cctaaatgat
     3001 aattaaaaat atgctacatc cttacttcct ctggaaagac ctgtaatacc tgttcttgca
     3061 agttccagta tctcatatcc tcgaagaaga ttcagaaaag cttccagttt actctgagtt
     3121 ccggtgagtt cgatgatcag cgagtcctct tcgacatcaa ctatcttcgc tctgaaaata
     3181 tctgccactg aaataatctc gcccctttgg gatgcgtttg cccgcagttt taccatgatc
     3241 agttcgcggt atactgactc tccgtctttc aaaactttta tatcaacgac atcttccagc
     3301 ttccttacct gtttttctat ctgggataaa atcagctcat cccccgatgc aactactgta
     3361 atcctcgtaa acctcgtatc ggtcgtcgca cctgccgtga tgctgtctat actgtatccg
     3421 cgtctgctga ataaccctgc aatacggctc aacactcccg gattattgtc tacaagcaat
     3481 gaaaatacct gacttctcat gtgttcctct ccttcttctc taaaaaaaca gagtgcgttc
     3541 tgtttttata ttttataata ttaccaactc agctattctt tgtcaagatg ttgtgctaaa
     3601 atttttacat atttgatgac cgttcagtca gtctaaaatc aagaatgaaa tcaaaagtta
     3661 tacgcagccg atcttttctg caatctcgtt taaataaacc catctttctg ttttttcgtc
     3721 aagctgtgcc tgtactttct cctgtt
//
LOCUS       DBMDXF010000179         3722 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5696_length_3722_cov_1.792875, whole genome shotgun sequence.
ACCESSION   DBMDXF010000179 DBMDXF010000000
VERSION     DBMDXF010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_3722_cov_1.792875"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="ACZPD4_07155"
     CDS             <1..1306
                     /locus_tag="ACZPD4_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378869.1"
                     /translation="RVLVITKDNDFDRTRQAISFGVDEYLLKPVKKVQIAKALQMIED
                     KLAGELVIETALSVENIFMACLNGQIHRDEIVDGITHEKYGFTLADRGNLFVVWLGNG
                     YMEQREMTQKILEKLGQNQEFSVCVLPIDEWHLFTVIIYGPEKKTIRYPFFKNEVVSR
                     LSGMIRGELVCLWEDVQHLEMLNVSLKNLRMIREWNLFFDRGDLIRREDVESMEIVPL
                     KYPAELENRLRRSAVISNRKEIIKCYYTLYDLCKRDPHSPSQIKEVLIRFNMAVLNAY
                     KLKREIHSELQVQKSMQDIAKAMSWGEIRAAVELFFRTLNFQAEEENENADLSPLIQK
                     ALQMVKKEYDQGITLEEMADKLFVSEEYLSSQFKKEIGVGFKEMVRTLQIERIKELLT
                     NTKLKLNQIAELAGYSDAKYMSRVFKDEVGVLPSEFRKSAHC"
     gene            1491..1718
                     /locus_tag="ACZPD4_07160"
     CDS             1491..1718
                     /locus_tag="ACZPD4_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1378870.1"
                     /translation="MSEMKITFKHPDEILDFVNTVSKYEFDMDMKKGHVVVDAKSLLG
                     IMHLGINNEIALQMYTDDCEELCREIAKYAA"
     gene            1983..3530
                     /gene="guaA"
                     /locus_tag="ACZPD4_07165"
     CDS             1983..3530
                     /gene="guaA"
                     /locus_tag="ACZPD4_07165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1378871.1"
                     /translation="MKNELVIVLDFGGQYNQLVARRVRECNVYCEIYSYKTDIEKIKA
                     MNPKGIILTGGPNSCYLPDSPTYTEELFKLGIPVLGLCYGAQLMMHVLGGEVKKADVR
                     EYGKTEVLIDKTSSKIFDNVSPSTICWMSHFDYISRIAPGFEISAHTADCPVAAAENA
                     EEKLYAIQFHPEVLHTVEGTKMINNFVKNVCGCTGDWKMDAFVESTIEAIRAKVGDGK
                     VLCALSGGVDSSVAAVMLSKAIGKQLTCVFVDHGLLRKDEGDEVEAVFGPEGPYDLNF
                     IRVNAQERFYAKLKGVEEPEEKRKIIGEEFIRVFEEEAKKIGAVDFLVQGTIYPDVVE
                     SGLGGESAVIKSHHNVGGLPDYVDFKEIIEPLRDLFKDEVRKAGLELGIPEYLVFRQP
                     FPGPGLGIRIIGEVNAEKVKIVQDADAIYREEIANAGLDRSIGQYFAGLTNMRSVGVM
                     GDERTYDYAIALRAVNTVDFMTAEAAEIPYEVLNKVMSRIINEVRGVNRVMYDLTSKP
                     PGTIEFE"
BASE COUNT         1256 a          554 c          910 g         1002 t
ORIGIN      
        1 cagagtgctt gtaattacaa aagataatga ttttgacagg acaagacagg caattagttt
       61 tggagtggac gaatatttgc tgaaaccggt gaaaaaagtg caaattgcaa aagcgctgca
      121 gatgattgag gacaagttgg cgggagaact tgtgatagaa acagctcttt ccgtagaaaa
      181 tatttttatg gcatgtttaa acggacagat ccacagagat gagattgttg acggaattac
      241 acacgaaaag tatggattta cgcttgccga tcgcggaaac ctgtttgtgg tatggcttgg
      301 aaacggctat atggagcaga gggagatgac gcaaaaaata ttagaaaaac ttggacagaa
      361 tcaagaattt tcggtttgtg tgcttcctat tgatgaatgg catcttttta ctgtgatcat
      421 atatggaccg gagaaaaaaa caatacggta tccttttttc aaaaatgagg tagtgtcacg
      481 attaagcgga atgatccgag gggaactggt ctgtttgtgg gaggatgtgc agcatttaga
      541 aatgttgaat gtatcactta aaaatctacg gatgatacgg gagtggaatc ttttctttga
      601 cagaggagat ctgatcagac gcgaggatgt agaatctatg gaaatcgttc ctcttaaata
      661 tccggcagaa ctggagaaca ggctgcgccg gtcggcagtt atttccaatc gaaaagaaat
      721 cataaagtgt tattatacgt tgtatgatct gtgtaaacgc gacccgcata gtccgagtca
      781 gataaaagaa gttttgattc gttttaacat ggctgttttg aacgcatata agctgaaaag
      841 agaaattcat tcggaactgc aggtgcaaaa aagcatgcag gatattgcga aggcgatgag
      901 ttggggagaa attcgcgccg cggtagaatt atttttcagg acactgaatt ttcaggcaga
      961 ggaagaaaac gaaaatgccg atttaagtcc gctgattcaa aaagcgctgc agatggtaaa
     1021 aaaggaatac gatcagggga ttaccttgga agaaatggca gataaattat ttgtatcaga
     1081 agaatatttg agcagtcagt ttaaaaaaga aatcggggta ggctttaaag aaatggttcg
     1141 tacattacaa atagaacgaa tcaaagaact gcttaccaac acgaaactga aattgaatca
     1201 gattgcggaa ttggcaggat attcagatgc aaaatacatg agcagagtat tcaaagatga
     1261 agtgggcgta ctgccaagtg aattccgtaa atcggcgcat tgctgatacg ataaatgcat
     1321 taaaaaagta ctcttttata aagaaattca cccttttaac tttggaaaaa tatgataata
     1381 tgaggtctgg aaattacaga tgattgtttt tagggaaaaa tagaaagaaa atattctaaa
     1441 aatataaaaa caattaaatt tcgcataaat attcaaagga gaggtgaaaa atgagtgaaa
     1501 tgaagattac attcaagcat cctgatgaga ttcttgattt tgtaaacacg gtctccaagt
     1561 atgaatttga catggatatg aaaaaaggac atgtagtcgt agatgcaaag tcattacttg
     1621 gaattatgca tcttggtatt aataatgaaa ttgctttgca gatgtacaca gatgactgtg
     1681 aagaactttg cagagaaatt gcaaaatatg cagcataaga aattttatgt ttaaagagat
     1741 gtgtatgaaa ggttgtccga atcttgaaat tacaatcttg caaataaaac atgaatagaa
     1801 cttgaattaa aaccgaatta aaaccgaaac gatggaaact acaaaatagt tgagaataat
     1861 aaattcctga cgtataataa aatacgttgg gaatttttgt tgcacttgta aaaacggaaa
     1921 cgcccaacta taatgagtac ataaaaagcg gttatcatgc cgtgttaaac aggaggacaa
     1981 tcttgaagaa tgaacttgtg atcgtgcttg atttcggcgg acagtataac cagcttgttg
     2041 ccaggcgcgt aagagaatgt aatgtatact gcgagatcta ttcttacaaa acagatattg
     2101 aaaagatcaa agcaatgaat ccgaagggaa tcatcctgac aggaggaccg aatagctgct
     2161 atctgccgga ttcacctacc tatacagagg aattatttaa actgggcatt ccggtacttg
     2221 gcttgtgcta cggtgcacag cttatgatgc atgttcttgg cggggaagtg aagaaagcag
     2281 atgtgagaga atatggaaaa acagaggttt tgattgataa aacttcttca aagatttttg
     2341 ataatgtttc accatctaca atttgctgga tgagtcattt cgattacatt tcccgaattg
     2401 ctccgggatt tgaaatatcc gcacacacgg cagactgtcc tgtagctgcc gcagaaaatg
     2461 cagaagagaa gctttatgca attcagtttc atccggaagt gcttcatacg gtagaaggaa
     2521 cgaagatgat caataatttc gtgaaaaatg tgtgcggctg taccggagat tggaagatgg
     2581 atgcgtttgt agaaagcacg attgaagcaa tccgcgcaaa agtcggagac ggaaaagtgc
     2641 tgtgcgctct ttcgggaggc gtagattctt ctgttgctgc agttatgttg tcaaaagcaa
     2701 tcggaaagca gcttacttgc gtatttgtcg atcatggttt gctgagaaaa gatgaaggcg
     2761 atgaagttga agccgttttc ggaccggaag gaccgtatga cctcaatttt attcgtgtca
     2821 atgctcagga aagattttat gcaaaattaa aaggtgtgga agagccggaa gaaaaacgta
     2881 aaattatcgg tgaagaattt atacgtgtat ttgaagaaga agcaaagaag ataggtgcag
     2941 ttgattttct tgtgcaagga acaatctatc cggatgtagt agaaagcggt cttggaggcg
     3001 agtctgcggt tattaagtcg catcataatg taggcggctt gccggattat gttgatttta
     3061 aagaaattat agaaccgttg cgagatcttt tcaaagatga agtgagaaaa gccggattag
     3121 aattaggaat tccggaatat cttgtgttcc gtcagccatt ccccggtccg ggacttggta
     3181 tccgcatcat cggagaagtg aacgctgaaa aagtgaaaat cgtgcaggat gcggatgcca
     3241 tctaccgtga ggaaatcgca aatgcaggat tagatagaag tatcggccag tatttcgcgg
     3301 ggcttacaaa catgagaagc gtcggtgtta tgggcgatga gagaacttat gattatgcta
     3361 ttgcactcag agcggtgaat acagttgatt ttatgactgc ggaagcagca gagattccgt
     3421 atgaagtgct taataaggta atgagcagaa ttatcaatga agttagaggc gtgaatagag
     3481 ttatgtatga tctgacaagt aagccgccgg gaacgattga gttcgaataa gttggagtat
     3541 caaagaaacc cagtatttat gcggggttgc ggcacttcaa gaagtctttt gataacaaat
     3601 tgataacagt tgcataagag ccattattct tgtaatccaa tggttttgcg gttaaagaat
     3661 gattgacagg tggttgtgca acaaataaat ccatattata aactaagccc ttagctgtgg
     3721 gt
//
LOCUS       DBMDXF010000180         3707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5719_length_3707_cov_1.628674, whole genome shotgun sequence.
ACCESSION   DBMDXF010000180 DBMDXF010000000
VERSION     DBMDXF010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5719_length_3707_cov_1.628674"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(14..916)
                     /locus_tag="ACZPD4_07170"
     CDS             complement(14..916)
                     /locus_tag="ACZPD4_07170"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1378872.1"
                     /translation="MMDKMKEKKTKKRTLKKEEKKESETEYVIRAENVDYTYEGSKIK
                     ALDHLSLNIRKGKKVAVMGGNGCGKSTFFLCLNGIRRPESGRIFIDGKPIEYTKKGLL
                     DVRRKVGIVFQNPDEQLFSASVYEEISFGILNMGADEETAEKAVRQIIEELGLSAFQE
                     EPTHALSGGQKKQVAIADILVMHPEVMILDEPAAALDMKHTKKVNEMIERLTEKGITV
                     LIATHDIEYACGWADEIVLMHEGKVIRHAETMEICQDEEALKEAELEIPTVIRMYRKL
                     TEKKILSDKEEPPRTWEELESRLT"
     gene            complement(913..1686)
                     /gene="cbiQ"
                     /locus_tag="ACZPD4_07175"
     CDS             complement(913..1686)
                     /gene="cbiQ"
                     /locus_tag="ACZPD4_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR1378873.1"
                     /translation="MIIIDKLCYRSKLRYVSAAEKLLYAVSSLMICVLTRSYAAALIV
                     FIANGILTVGKGKIPLSCYVRLLMIPIVFLIVGTGAVFLNLSKVPMDAFAVPVGEIYI
                     TASRESAFWAVNLCITALAAVTCLYFLSLNTTMTDILGALRKMHMPRLLIELMMLIYR
                     FVFILLHTASAIMTAQEARLGNRDYRTQLRAFGKMLTALFFISLRKANALYDAMEARG
                     YDGTINVLERNTPAKKKEMILLAVFELVLIIVAAAERMI"
     gene            complement(1708..2058)
                     /locus_tag="ACZPD4_07180"
     CDS             complement(1708..2058)
                     /locus_tag="ACZPD4_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1378874.1"
                     /translation="MSKNKKTVILLLIAAVLIAVVPLFVLKEAEFGGSDDAGSVMVEE
                     IHGEYEPWFTPVLETALDRELPGEIESLIFCIQTGIGVGIIAFIMGRFVERKKWTDSL
                     GDNAFEVLREKQKS"
     gene            complement(2051..2818)
                     /locus_tag="ACZPD4_07185"
     CDS             complement(2051..2818)
                     /locus_tag="ACZPD4_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR1378875.1"
                     /translation="MSKKEKILLKASIVFAAVFAIVPAANAMHIMEGYLPAKYCVAWG
                     VVCLPFLIAGFISLKNKVKENRRNLTLIAMSGAFIFVISSLKIPSVTGSCSHMTGTGL
                     GAILFGPASVSVLGVIVLLFQALLLAHGGLTTLGANTFSMAIAGPVVSFGIYKLCQKI
                     NVNRRVGIFLAAFLGDLFTYCVTSIQLAFAYPSEAGGVGASAVKFLGVFAPTQIPLAV
                     IEGILTVVIVIGLETYAKPELRSIGFFKNKKGETQDE"
     regulatory      complement(2852..3045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3102..3629)
                     /locus_tag="ACZPD4_07190"
     CDS             complement(3102..3629)
                     /locus_tag="ACZPD4_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1378876.1"
                     /translation="MERGLIHIYCGDGKGKTSSAVGLAVRAAGCKKKVLFARFLKNEN
                     SGELNILDSISDIEVLHLEKSYGFYKTLTEKEKKEVKKLYEALWNEVLRKVSGGSYDV
                     LVMDEFMAAYRYGLIPNEEALCFLEKKPTGLEVVLTGRDPDLRLVEKADYVSEICKVK
                     HPYDRGIRARRGIEY"
BASE COUNT         1020 a          965 c          585 g         1137 t
ORIGIN      
        1 caaacttttc agatcaagta agccttgact ccagttcttc ccatgtgcgc ggaggctctt
       61 ctttgtctga taagattttt ttctctgtta atttgcgata catacgaatg actgtcggta
      121 tttccaattc tgcttctttc aacgcttcct catcctgaca gatttccatc gtctctgcat
      181 ggcgtatcac ttttccttca tgcataagca caatctcatc tgcccatcca caggcatact
      241 ctatatcatg tgttgcgatc agcaccgtaa ttcctttttc cgtcagcctc tcgatcatct
      301 cattcacttt tttcgtatgt ttcatatcaa gcgccgccgc aggttcgtct aaaatcatca
      361 cttcaggatg cataaccaga atgtctgcaa tcgcaacctg ttttttctgt cctccgctta
      421 aagcatgagt cggctcttcc tgaaacgcag aaagccccag ttcctcgatt atctgtctga
      481 ccgccttttc ggcagtttct tcatccgccc ccatgttcaa tatcccgaaa gagatttcct
      541 cataaacact tgcagaaaaa agctgttcat ccggattttg aaatacgatc ccgacttttc
      601 gtctgacatc taaaagtcct ttttttgtat attcgatcgg ttttccatct ataaaaatcc
      661 ttccggattc cggtcttctg atcccgttca ggcaaagaaa aaaagtagat tttccgcagc
      721 cgtttcctcc cataaccgcg acttttttcc ctttacgaat gttcaaactc aagtgatcca
      781 atgcctttat tttacttccc tcatatgtat aatctacatt ttctgcacga atcacatatt
      841 ccgtttctga ttcttttttt tcttcctttt tcaatgttct cttttttgtt ttcttttctt
      901 tcattttatc catcatatca tccgttccgc cgcagctaca attatcaaaa caagctcaaa
      961 tacagccaac aatatcattt ctttcttttt cgccggagtg ttcctttcta gcacattgat
     1021 tgttccgtca taccctcttg cttccattgc atcatacagc gcatttgctt ttctcaaaga
     1081 aataaagaac aaagcggtca gcatctttcc gaatgcacga agctgtgtcc gataatctct
     1141 atttccaagt cttgcctcct gtgctgtcat aattgcagac gcagtatgaa gaagaataaa
     1201 tacaaaacga tagatcaaca tcatcaactc aatcagaagc ctcggcatat gcattttcct
     1261 gagcgctccc agtatatctg tcatcgttgt gttcaacgac agaaaataaa gacatgtcac
     1321 tgccgccagc gccgtgatac ataaattgac tgcccaaaaa gccgattcac ggcttgccgt
     1381 tatatatatt tccccgaccg gcacggcaaa tgcatccata ggaactttcg atagatttaa
     1441 aaaaacagct cctgttccga cgatcaaaaa tacgatcgga atcatcagca gtcgaacata
     1501 gcaggaaaga ggaatcttcc cctttcctac cgtcaaaatt ccatttgcga taaatacgat
     1561 caaagccgcc gcataggagc gggtcaaaac acagatcata agagaagaaa cggcatacag
     1621 taacttctct gccgcactga catatcttaa ttttgaccgg taacacaatt tatctataat
     1681 gatcattcat cgctcctttt tattctccta tgacttttgt ttttccctca aaacttcaaa
     1741 agcattatcg cccagactat ccgtccattt tttacgttcc acgaaccgtc ccattataaa
     1801 tgcgatgatt cccacgccta tccctgtctg aatacagaaa ataagacttt ctatctctcc
     1861 gggcagttct ctgtctaacg cagtctccaa aaccggagta aaccacggct catattctcc
     1921 gtgaatttct tctaccatca cgcttcccgc atcatcagaa ccgccgaatt ctgcttcttt
     1981 taaaacgaaa agaggaacaa cggcgattaa aacagcggcg atcaataata aaatcactgt
     2041 ttttttgttt ttactcatct tgtgtctccc cctttttatt tttgaaaaat cctatacttc
     2101 ttaattccgg ttttgcatat gtttccagac ctatcacaat gacaactgta aggatacctt
     2161 ctatcacggc aagcggaatc tgtgtaggcg caaatactcc gagaaactta acagccgacg
     2221 ctccgacgcc tcccgcttca gacggatagg caaaagcaag ctggatactt gtcacacaat
     2281 atgtaaacag atctcctaaa aatgcagcca aaaaaatacc gactcttctg tttacattta
     2341 ttttctgaca taatttataa attccaaaag agacgaccgg tcctgcgatc gccatggaaa
     2401 atgtatttgc tccaagtgtt gtcaaacctc cgtgagctaa aagcaatgcc tgaaaaagca
     2461 atacaatgac accgagcacg ctcacagatg ccggtccaaa caaaatcgcg ccgagccctg
     2521 ttcctgtcat atgcgaacaa ctccctgtaa cagaagggat cttaagcgat gaaataacga
     2581 aaataaacgc tcccgacatt gcgatcagcg ttaaatttct tctattttct tttactttgt
     2641 ttttcaatga aataaatcct gcaatcagaa acggcaggca gacgacgccc caagcaacgc
     2701 agtatttagc cggaagatac ccttccataa tatgcattgc attcgctgcc gggactatgg
     2761 caaataccgc cgcaaacacg atagatgcct ttagtaagat tttttctttt tttgacatgt
     2821 tttttcctct ccttgatcat aatcccgtaa tctacggact catttgaaag tgaatattct
     2881 gacttaggga tcgatgcaaa tattccgcct tcccacagaa tcttattccg cagtggcaat
     2941 atggaatacc gctcctccaa tacagcaacg ggtattgtgc aggattctca cctgacttcc
     3001 tcacagatat ctcaaagtaa cctaacctta tctaaagtcc ggtacaaaga gcaatctata
     3061 cttctctatc tgtttatctt tcaaacgtat gccgagcccg actaatactc aatccctctt
     3121 ctagcccgga ttccccggtc gtaagggtgt ttcaccttgc agatctcaga tacataatcc
     3181 gctttctcta caaggcgaag gtcaggatcc cgtcctgtta agacaacttc cagacctgtc
     3241 ggtttctttt ccagaaaaca aagagcttcc tcgtttggta tcaatccata ccggtatgcc
     3301 gccataaatt catccataac aagtacgtca taagatccgc cggaaacttt tctcaagacc
     3361 tcattccaca gagcttcata tagcttcttt acctcttttt tttctttttc tgtcagtgtt
     3421 ttatagaaac cgtaagattt ctccagatgt aaaacttcaa tgtccgaaat gctgtcgaga
     3481 atgtttaatt ctcccgaatt ctcattttta agaaaacgtg caaaaagcac tttctttttg
     3541 cagcccgcag ctctgaccgc cagcccgacg gctgaggacg tctttccttt gccgtctcca
     3601 caataaatat gaatcaaacc tcgttccatc gtcttttacc tctctgtaaa aatcctttat
     3661 attttaccac aaatatacac tctgtgacaa cattttgtgt tatacta
//
LOCUS       DBMDXF010000181         3692 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5753_length_3692_cov_1.600947, whole genome shotgun sequence.
ACCESSION   DBMDXF010000181 DBMDXF010000000
VERSION     DBMDXF010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5753_length_3692_cov_1.600947"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            38..1318
                     /locus_tag="ACZPD4_07195"
     CDS             38..1318
                     /locus_tag="ACZPD4_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HIR1378877.1"
                     /translation="MIIGIDGVQWIPENVGEIVAFKRGLSDGSCVLIESTANQCDQNG
                     GYTGMTPADFKKFVLEIADRNGFDRNRIFLGGDHLGPLTFAYKDEAEAMADSEELIRH
                     YVAAGFTKIHIDTSMKVNSDPEDVRLSDEIIAGRGAHLAKVAEETYKELLERDPEAIP
                     PVYVIGSEVPIPGGAVGAEDNGVQVTKVEDFKNTVAAFEKAFAKEGLDKDVWERVIGV
                     VVQPGVEEKDSGCTEYDREKAKDLMAAIKDFPTLVFEGHSTDYQTKIKLRELVEDGVG
                     ILKVGPGLTFAMREAMFALENIEKELIYGTDTEPSKFAEVLDAEMLKNDKNWKKHYQG
                     TELEIRLKRKYSFSDRCRYYMPTPAVEAAADRLLTNLRTLGIPLNLLSQFMPIQYTKV
                     REGYLKNDPVELIEDRIINTIDEYLYGTHQNELL"
     gene            1582..2925
                     /locus_tag="ACZPD4_07200"
     CDS             1582..2925
                     /locus_tag="ACZPD4_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR1378878.1"
                     /translation="MGFSIETGISAFTVFMQGLLSFFSPCVLPLVPLYLGYLSGGLNT
                     AVRTGENSEKNKDRLKLFLRVLFFTVGIGSAFFILGLGASAAGSFFHEQRMLFARVGG
                     ILVVLFGLYQLGVFGSSKVLGSEHRFPIRLEKMTMSPITAFIMGFAFSFAWTPCVGPA
                     LTSVLLMAGSAESGAKGFLLIGVYTIGFILPFLLAGIFTARLLDLFKKHMKIVRYTVK
                     IGGVLMVLMGLLMFTGKMNDITGYLSSAGQKTESGKQENGKKQDEKDASGGDEEQTSG
                     KDTSGENASGKADKEEKDQDPEKVRAHMGELKDQFGKSHKLEDYKGKVIFLNFWATWC
                     GPCRSEMPEIQKLYEEYTVMGNDSEVVILGVAGPGIGQEGTVSDITKFMEENGYTYPV
                     LMDETGELFEWYGISAFPTTYMIDRDGNLYGYVTGQLTEDIMRSIIKQTLEGKAP"
     gene            2947..>3692
                     /locus_tag="ACZPD4_07205"
     CDS             2947..>3692
                     /locus_tag="ACZPD4_07205"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1378879.1"
                     /translation="MVIIEKENFSVSQICSSGQCFRLDPADETGKVYELTAGDRYLKI
                     EVAEDQKKVRFYCTQEEFEQFWKTYFDLDTCYADYLSLIKDDEYLKKAAGFGSGIRIL
                     QQDLWEMIVTFILSQQSNIPRIKSMIQRISERYGDKKETGEGHVYYAFPRAEQLVQAS
                     EEELRALKLGYRSKYLCNTAEMVAEGTVNLEKIKEMSYDEAKKELLKLSGIGSKVADC
                     ICLFALHKLDAFPVDTHIQKVIDTVYAGKFP"
BASE COUNT         1211 a          595 c          954 g          932 t
ORIGIN      
        1 agcatttaaa aagtgccgca aaaagtggga tgaggaaatg ataatcggaa ttgacggtgt
       61 gcagtggatt cccgaaaatg tgggagaaat cgttgcattt aaaagaggac tttcagacgg
      121 atcctgtgtt ttgattgaga gtacggcaaa ccagtgtgat caaaacggcg gatatacagg
      181 tatgacacct gccgatttta aaaaatttgt tcttgagatt gctgatcgta acggatttga
      241 ccgtaacaga atcttccttg gaggagatca cttaggaccg cttacattcg cgtataaaga
      301 tgaagcagag gcgatggctg attcggaaga gctgatccgt cactatgttg cagcaggatt
      361 tacaaagatt catatcgata caagtatgaa agtaaacagc gatccggaag acgttcgtct
      421 gtccgacgag atcattgcag gacgcggcgc tcatcttgca aaagttgcgg aagagacata
      481 taaagagctg ttggagcgtg atccggaagc aattccgccg gtatatgtaa tcggaagtga
      541 agtgccgatt ccgggaggag ctgtcggagc ggaagataac ggagttcagg tgacgaaagt
      601 agaagatttt aagaacacag ttgcggcatt tgagaaagct tttgcaaaag aaggtcttga
      661 caaagatgta tgggagcgtg taatcggagt tgttgttcag ccgggtgtag aagaaaaaga
      721 cagcggatgt acagaatatg atcgtgagaa agcaaaagat ctgatggctg cgatcaaaga
      781 tttcccgaca ttggtatttg aaggacattc tacagattat caaacaaaga tcaaactgcg
      841 cgagcttgta gaagacggag tcggcatttt gaaagtaggt ccgggactta catttgctat
      901 gcgtgaagca atgttcgcac tggaaaatat cgaaaaagag ctgatatacg gaactgatac
      961 agagccgagc aaatttgcgg aagttcttga tgcggagatg cttaaaaacg ataaaaactg
     1021 gaagaaacat tatcagggaa cagagcttga gatccgtctg aagagaaaat attctttctc
     1081 agaccgctgc agatactata tgccgacacc ggctgtagaa gctgccgcag atcgtctgct
     1141 tacaaacttg cgtacactcg gaattccgct gaacctgctc agccagttca tgccgattca
     1201 gtatacgaaa gtacgtgaag gatacttgaa aaatgatccg gtagaactga tcgaagaccg
     1261 tatcatcaat acgatcgacg agtaccttta cggaacacat cagaacgaac ttctctaaaa
     1321 agaagaacag aaaaataacg aagcgtcttg gaacaaaaag ttttgttccg agacgctttt
     1381 ttgataagag aaaaactcga cagctttgac cttaaagact cgaaagtaag aaacaggctg
     1441 gctttgtgat aatatcactg tctcatatat gaaaaatatg ttataaatta tttaaaaaga
     1501 aaagaaaacg tacaaagcga agcaagtaca aaagaaagac ggaaagaaaa gataaataat
     1561 aaaaagaatg gagagaaaca tatgggattt tcgatagaaa ccgggatttc ggcttttaca
     1621 gtttttatgc aggggctatt aagcttcttt tccccttgcg tattgccgtt agtaccactt
     1681 tatcttggct atttgtcagg cggtttgaat actgcggtca gaacgggtga aaacagtgag
     1741 aagaacaaag atcggctgaa actgttttta agagttttgt tttttaccgt aggcatagga
     1801 agcgcatttt ttatactcgg tctgggcgcg tcggcggccg gaagtttctt ccatgagcag
     1861 aggatgctgt ttgccagagt cgggggaata ttagtcgtat tatttggtct gtatcagctt
     1921 ggagtatttg gaagttccaa agttctcgga tcagaacata gatttccgat tcggcttgaa
     1981 aaaatgacga tgtcgccgat cacagctttt attatgggat tcgcatttag ttttgcatgg
     2041 acgccgtgtg taggccctgc gttgacaagc gttctcctta tggctggaag tgcagagtcg
     2101 ggagcgaaag gttttctttt gatcggagtg tatacgatcg gatttatttt gccgttttta
     2161 ttggcaggca tttttactgc acggctttta gatcttttca aaaagcatat gaagatcgtc
     2221 cgttatacag tgaaaatcgg cggagtactc atggtgctga tgggactgct tatgtttaca
     2281 gggaaaatga atgatattac aggatatttg tcttcggcag ggcagaaaac agaatcggga
     2341 aagcaggaaa acggtaaaaa gcaggatgaa aaggatgctt caggagggga tgaagagcag
     2401 acatccggaa aagatacgtc cggagaaaat gcaagtggga aagcagataa agaggaaaaa
     2461 gatcaggacc cggaaaaagt ccgtgcacat atgggtgaat taaaagatca gttcggaaaa
     2521 tctcataagt tggaggatta caaaggaaag gttatcttct tgaatttctg ggcaacgtgg
     2581 tgtggaccat gccgcagcga aatgccggaa atacagaaac tgtatgaaga atacaccgtt
     2641 atggggaatg attcggaagt ggtgatctta ggagtcgcgg gacccggaat cggacaggaa
     2701 ggaaccgttt cggatattac gaaatttatg gaggaaaacg gatatacata tccggtactt
     2761 atggatgaaa cgggagaact gttcgagtgg tacggaattt cagcatttcc gacgacatat
     2821 atgatcgatc gtgacggaaa tttatatgga tatgtgacag gacagcttac ggaagatatt
     2881 atgagaagca ttataaaaca gacgttagaa ggaaaagcac cgtaaggaaa agataaggaa
     2941 gagacgatgg ttataataga aaaagaaaat ttttccgtgt cgcagatttg ttcttcgggt
     3001 cagtgtttca gacttgatcc ggcagatgaa acgggaaaag tatacgaatt gacggcggga
     3061 gacagatatt taaaaatcga ggtggcggaa gatcagaaaa aagtaagatt ttactgtaca
     3121 caagaagaat ttgaacaatt ttggaaaacg tattttgacc tcgatacatg ttatgcagat
     3181 tatctttcgc ttataaaaga cgatgaatat ttaaaaaagg cggcaggatt tggaagcggt
     3241 atccgtattt tgcagcagga tttatgggaa atgatcgtaa catttatttt atcccagcag
     3301 agcaatatac cgaggatcaa atcgatgata caaagaatca gtgaacggta tggagataaa
     3361 aaagaaaccg gcgaaggaca tgtatattat gcatttccga gagcagagca gcttgtacag
     3421 gcgtccgaag aagaacttcg ggcgcttaag ctcggttata gaagcaaata tttatgcaat
     3481 actgcggaaa tggtggcaga gggaactgtt aatcttgaaa aaataaaaga gatgtcttat
     3541 gatgaagcaa agaaagaatt gctgaaactt tcaggtatcg gaagcaaggt tgccgattgt
     3601 atctgtttgt ttgcacttca taagctcgat gcatttccgg ttgatacaca tatacaaaaa
     3661 gtaattgaca ccgtatatgc cgggaagttt cc
//
LOCUS       DBMDXF010000182         3690 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5758_length_3690_cov_1.860685, whole genome shotgun sequence.
ACCESSION   DBMDXF010000182 DBMDXF010000000
VERSION     DBMDXF010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_3690_cov_1.860685"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="ACZPD4_07210"
     CDS             <1..1061
                     /locus_tag="ACZPD4_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1378880.1"
                     /translation="DRAADKVGVNFIGGYSALVSKGMTKSDENLIRSIPQALAQTERI
                     CSSVNVGSTKTGINMDAVRLCGEIVKATAEETKERDSLGCAKLVIFCNAPDDNPFMAG
                     AFLGVTESDAVINVGVSGPGVVKKALEKVRGKGFEELCETIKKTAFKVTRVGQLVAGE
                     ASQRLGIPFGIVDLSLAPTPAIGDSVAEILEEIGLERVGAPGTTAALAMLNDQVKKGG
                     VMASSYVGGLSGAFIPVSEDQGMIDAVHAGSLTLEKLEAMTCVCSVGLDMIAIPGHTS
                     AATLSGIIADEMAIGMVNQKTTAVRLIPVIGKDVGDTAEFGGLLGYAPIMPVNEFRCD
                     AFVSRKGRIPAPIHSFKN"
     gene            1133..1996
                     /locus_tag="ACZPD4_07215"
     CDS             1133..1996
                     /locus_tag="ACZPD4_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1378881.1"
                     /translation="MSKIAVVTDSNSGITQERAKELGIYVLPMPFFIDGELYLEDITL
                     TQEQFYEKLRADSDISTSQPSPGNVMELWESVLKEYDEIVCIPMSSGLSSTCETAMSI
                     AREYKGKVYVVDNQRISVTQEQSVYDAMYLRDKGMCAEEIKNILEKEKLQASIYITVD
                     TLKYLKKGGRITPAAAAIGTVLNLKPVLQIQGEKLDAFAKVRGWKVAKKTMLNAIEKD
                     LNERFADVKEDIILGMAYTCSKEEAMEWKQEIMNRFPGYEFLEAPLSLSVSCHIGPGA
                     MAVTCIKKVSR"
     gene            complement(2111..3436)
                     /locus_tag="ACZPD4_07220"
     CDS             complement(2111..3436)
                     /locus_tag="ACZPD4_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1378882.1"
                     /translation="MKSKQITTEDKFNNKWGFVISCIGSSVGMANIWLFPYRAGEFGG
                     AAFLIPYIIFVVLIGFTGVIGEMAFGRAMQSGPLGAFKKAYEMKGSKLGTFIGLIPVI
                     GSLGIAIGYSVIIGWILKFFADSITGKIISEEDPSAIFGELSRDFGSIPWHLLGLFLV
                     LLCMSFGVARGIEKVNRIMMPAFFVLFLFLAVRVAFLDGSAPGYSYLFKPDWSEMANP
                     RTWVYAMGQAFFSLSLAGSGTVVYGSYLKSDVDVVSSAKYVAFFDTAAALLAGLVIIP
                     AVFSFGMAPNSGPGLLFITIPAVIRDIPLGRVFAIIFFLAVLFAGITSLMNLLESPVE
                     ALQKRLGWSRRSAITFIGVITAAVGIFVEGGLLSAWMDSISIYVIPLGALLAGITMFW
                     ICGDKFARNAAQLGRVRTIGKWFKPMTKYVFCGVAVLVYILGIFFGGIG"
BASE COUNT         1137 a          697 c          941 g          915 t
ORIGIN      
        1 tggatagagc tgcagataaa gtaggggtca attttatcgg tggttattcg gctctggtat
       61 ccaagggaat gacgaaaagt gatgagaatc tgattcgttc gatccctcag gcgctggcgc
      121 agacagaaag gatctgcagt tccgtaaacg taggttctac aaagaccggg atcaatatgg
      181 atgcggtacg cctgtgcggc gagattgtaa aagcaacagc agaagagacg aaagaaagag
      241 attctctcgg gtgtgcaaaa cttgttattt tctgtaacgc tccggatgat aacccgttta
      301 tggcaggtgc gttcctcgga gtgacagagt cggatgcggt gattaatgtc ggtgtcagcg
      361 gccccggtgt tgtgaagaaa gcattggaaa aagttcgcgg aaaaggattt gaagagttgt
      421 gtgagacgat taaaaagaca gcgtttaaag ttacccgtgt cggacagctc gtggcaggag
      481 aggcgtctca aaggttaggc attccgtttg gcattgtgga tctttcgctt gcgccgactc
      541 cggcaatcgg agacagcgtt gcggaaattt tggaagaaat cggcttggaa agagtcgggg
      601 caccgggaac aacggctgct cttgcgatgc ttaatgatca agtgaaaaaa ggcggagtta
      661 tggcatcttc ttatgtagga ggtttgagcg gcgcatttat tccggttagt gaagatcagg
      721 gcatgatcga cgcagttcac gccggttcac ttacattgga aaaattagaa gctatgacat
      781 gtgtctgttc ggtaggactg gacatgatcg ccattccggg gcatacaagc gcagctacac
      841 tttccggaat tatagcagat gagatggcga tcggtatggt aaaccagaaa acaacggcgg
      901 ttcgccttat tccggtaatc ggaaaagatg taggagatac cgcggaattc ggaggacttc
      961 tcggatacgc tccgatcatg ccggtaaacg aattcagatg cgatgcattt gtgagcagaa
     1021 aaggaaggat cccggctccg attcacagct ttaaaaatta aacggaaaaa caagacagaa
     1081 ggaaataaat ggtctgagat agtcaggttt aaagtgataa agggagaatg atatgagtaa
     1141 gattgcggtc gtaacagaca gtaatagtgg tattacacag gaaagagcga aagaattagg
     1201 gatttatgta cttccgatgc ctttttttat tgatggtgag ttatatcttg aagacatcac
     1261 actgacacag gaacagtttt atgagaagct cagagcggat tcggatatat ctacttccca
     1321 gccttccccg ggaaatgtga tggaactgtg ggaaagtgtg ttgaaagaat atgatgagat
     1381 cgtatgtatt cctatgtcaa gcggacttag cagtacatgc gagacggcaa tgtctattgc
     1441 gagggaatat aaagggaaag tttatgttgt ggacaaccag agaatctcgg ttacacaaga
     1501 acaatctgta tatgatgcaa tgtatttgcg cgataaaggg atgtgcgcgg aagagattaa
     1561 aaatattctc gaaaaagaga agctgcaggc aagtatttat atcacagttg atacgttgaa
     1621 gtatttgaaa aaaggaggaa gaatcactcc ggcggctgcg gcgatcggaa ctgtattgaa
     1681 tttgaagccg gttcttcaga ttcagggtga aaaattagat gcatttgcaa aagtcagagg
     1741 gtggaaagtg gcaaagaaaa cgatgctgaa tgcaatcgaa aaagatctca atgagcgttt
     1801 tgcagatgtg aaagaagata ttatacttgg tatggcatat acatgttcaa aagaagaagc
     1861 gatggaatgg aagcaggaga tcatgaatcg ttttccggga tatgagtttt tggaagctcc
     1921 tctgtcatta agtgtttcct gccatatcgg tcctggagca atggcggtga catgtatcaa
     1981 aaaagtgtcg agataatgtt tcataaaaaa taaaatagaa agacaaaaac gcatctgcag
     2041 gatctgaata tccggcagat gcgcttttat aaatatcaaa ttttgaaaca ggcgctttcg
     2101 gctctttcaa tcagccgata ccgccaaaga aaattccgag aatgtagaca agtactgcaa
     2161 caccgcagaa tacatatttt gtcattggtt tgaaccattt tccgatcgtg cgcacgcgac
     2221 cgagctgagc ggcatttctt gcaaatttat ctccgcatat ccagaacatt gtaattccgg
     2281 caagcagtgc tccgagaggg attacataga tggagatact gtccatccat gcgcttaaga
     2341 gcccgccttc tacgaagatt ccaacagcgg cagtgatgac gccgataaaa gtgatcgcag
     2401 atcgacgaga ccagccgagc cgtttttgaa gcgcttctac cggactttct aaaaggttca
     2461 ttaaagaagt gattccggca aaaagtacgg cgaggaagaa gataattgca aaaactcttc
     2521 ctaacggaat atcacgtata acagcgggaa ttgtaatgaa caaaagtccc ggtccggagt
     2581 tcggcgccat gccgaaagag aatacggccg ggatgataac aagaccggct agtaatgcgg
     2641 ctgctgtgtc aaaaaaagca acgtatttgg cactgcttac aacatctaca tcagatttta
     2701 agtaacttcc gtaaacaaca gttccgcttc cggcaaggga aagggagaaa aatgcctgcc
     2761 ccatcgcgta tacccacgtg cggggattgg ccatttccga ccagtcgggt ttgaataagt
     2821 acgaataccc gggcgctgaa ccgtcgagga aagcaacgcg gactgcaagg aataaaaaga
     2881 gaacaaaaaa agcaggcatc atgatgcggt ttactttttc gattccccgg gcaactccga
     2941 atgacatgca gagcaagaca aggaaaagac ctaaaagatg ccacggaatg ctgccaaagt
     3001 cgcgggaaag ttctccgaat atggcagaag gatcttcttc actgataatt tttcctgtga
     3061 tcgagtcggc aaaaaatttc aagatccagc cgatgatgac gctgtaaccg attgcgattc
     3121 cgagcgatcc gataacgggg atcagcccga tgaatgtgcc aagttttgac cccttcattt
     3181 cataagcctt cttaaatgcg ccaagaggtc cggactgcat cgcgcgtccg aatgccattt
     3241 ctccgatcac tccggtaaat ccgataagga caacgaagat gatatagggg atgagaaatg
     3301 cggctccgcc gaattcacct gcgcggtaag ggaagagcca tatatttgcc attcctacag
     3361 agcttccgat gcaggagatg acaaaccccc atttgttgtt gaatttgtct tcagttgtaa
     3421 tttgttttga tttcatagtg ttaaactctc tttccttttc ttcttccgta cagatgtgct
     3481 tttatatacg gaagattcag caaaacaatc ttgggagtgc gtgctgatta tactatcata
     3541 tcatgattta aaagaagata aaacaataaa cgataagttt gtcatttttc atgtcggttc
     3601 cataatttgc ggaaagttaa agcggaaagt taagaaaaat aaagatgtca ataacttatg
     3661 aaaatgtcac tttttaatat agaaatttat
//
LOCUS       DBMDXF010000183         3667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5807_length_3667_cov_1.727987, whole genome shotgun sequence.
ACCESSION   DBMDXF010000183 DBMDXF010000000
VERSION     DBMDXF010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_3667_cov_1.727987"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="ACZPD4_07225"
     CDS             <1..214
                     /locus_tag="ACZPD4_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RapZ C-terminal domain-containing protein"
                     /protein_id="HIR1378883.1"
                     /translation="DKLKDMTEFLIPNYILEGKNQLVISIGCTGGKHRSVTLANALYK
                     VLEKQENYGVRIEHRDIEKDTITKRK"
     gene            219..1178
                     /gene="whiA"
                     /locus_tag="ACZPD4_07230"
     CDS             219..1178
                     /gene="whiA"
                     /locus_tag="ACZPD4_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1378884.1"
                     /translation="MSFSGKVREELSENISQARHCRIAELAAFIGMCGTVSIDSFDRY
                     HIKIHSENIVVARKVFTLLKKTFNIRTDISVKRNVKRESVSYLVVIKSHEDALRVLQA
                     TKMIGEQQVGTDAICGFNPLIIRQVCCRRAFLRGVFQAAGSMSDPNKSYHFEIVCTIS
                     EIAEQIRSVICSFGLDAKIVRRKKSYVVYLKEGSQIVDILNVMEAHVALMELENVRIL
                     KEMRNSVNRKVNCETANINKTVSAAVKQVEDITYLRDMIGFENMPDNLVEAAYARLDH
                     PDATLKELGESLTPPVGKSGINHRLRKLSEMAESLRQKQGGLL"
     gene            1175..1435
                     /locus_tag="ACZPD4_07235"
     CDS             1175..1435
                     /locus_tag="ACZPD4_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1378885.1"
                     /translation="MIKTPVVVKTEEDFDGRPIALLVQEASQYASTIYIQVGEKHINA
                     KSIMGMMSLSLADGEEITVVTDGEDEQRAAEGIKTFFAKGRK"
     gene            1539..2471
                     /locus_tag="ACZPD4_07240"
     CDS             1539..2471
                     /locus_tag="ACZPD4_07240"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1378886.1"
                     /translation="MKKMLFIYNPNAGTGKLKPKLADVLDIFTKSGYEVTVYPTQKRY
                     DAAEKIQESGSQYDLIVCSGGDGTLDEAVTGMQLCNCKVPLGYIPAGTTNDFATSLGI
                     PKDILEAAETAVNGTVFPCDVGMFGEDYFAYIAAFGLFTDVSYATDQNVKNVLGHMAY
                     ILEGAKRVFNIPSYKIKITHDGEVIEDEFIFGMVTNSRSVGGFKGIIGPEIVFDDGVF
                     EVTLIKTPKNAIELNELLGAIVMKQLDSKRMYSFKTGEIKFESEEEIPWTLDGEFGGA
                     HKEITVKDKKQAVQIKVDSEVAAGLSVDSEKVKK"
     gene            2578..2649
                     /locus_tag="ACZPD4_07245"
     tRNA            2578..2649
                     /locus_tag="ACZPD4_07245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2611..2613,aa:Glu,seq:ctc)
     gene            2878..3045
                     /locus_tag="ACZPD4_07250"
     CDS             2878..3045
                     /locus_tag="ACZPD4_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378887.1"
                     /translation="MLTDEFLERVFANKEMQKMPIGCQSTAVHAFQKVLEDMKEENPY
                     ADLSAILSSDE"
     gene            3008..3523
                     /locus_tag="ACZPD4_07255"
     CDS             3008..3523
                     /locus_tag="ACZPD4_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378888.1"
                     /translation="MQTYPQYYPQMNNYGQQYNPQQPYMDRLAGLQQYQQTLQQPQMA
                     GIQMSLPNQPIGLNGKVVDTIEQITANDVPMDGSVAIFPKKDMSEIYLKSWTPNGTIA
                     TVVYHPVMEESSNSASVQDNLKIGLSDEVTEIFMQKFDELANKIEELEKSMTKPMTKT
                     TVSRTKKESET"
BASE COUNT         1244 a          568 c          912 g          943 t
ORIGIN      
        1 ggacaaatta aaggacatga cagaatttct gattccgaac tatattctgg aaggtaaaaa
       61 ccagcttgtt atttcgatcg gctgtacggg aggaaaacat cgttccgtaa cgttggcgaa
      121 cgcgttgtat aaagttttgg aaaaacagga aaattacggg gtgcgtattg aacaccgcga
      181 tattgagaaa gatacaatta caaaaaggaa gtgagacgat gtctttttcg ggaaaagtca
      241 gagaagaact ttctgagaac atcagtcagg cgagacattg ccggatcgca gaactagctg
      301 cgtttatcgg catgtgcgga acagtatcga ttgacagttt tgacagatat catattaaga
      361 ttcattcgga aaatatcgtt gttgcaagaa aagtctttac attgttaaaa aaaacattta
      421 atataagaac tgatatctct gtaaagagaa atgtaaaaag agagagtgta tcatatcttg
      481 tcgtgataaa aagtcacgag gatgcgctca gagtccttca ggcaacgaag atgatcggag
      541 aacagcaggt gggaacggat gcgatttgcg gatttaatcc tcttataatc cgtcaggtct
      601 gctgcagaag agcatttctg cgaggcgtat ttcaggcggc aggctctatg agtgatccga
      661 ataaatcata ccattttgaa attgtatgca cgatttcgga gattgccgag cagatcaggt
      721 cggttatatg cagttttggt cttgacgcta agattgtacg cagaaaaaag tcttatgttg
      781 tttatttgaa ggaaggatct cagatagtag atatattgaa tgtaatggaa gcgcatgtag
      841 ctcttatgga attggagaat gtaaggattc tgaaagagat gcgtaattcc gtaaacagga
      901 aggtgaactg cgagactgcc aatattaata agacggtgtc cgcggcggta aaacaagtcg
      961 aggatattac atatctcaga gatatgatcg gttttgaaaa tatgccggat aatctggttg
     1021 aagcggcata tgcaaggctg gatcatcccg acgctacgtt aaaagaactg ggagagagtc
     1081 ttactcctcc tgtcgggaag tcggggatca atcacaggct gagaaaactg agcgaaatgg
     1141 cagagagtct caggcagaaa caaggaggat tattatgatt aagacacctg ttgtagtaaa
     1201 gacggaagaa gattttgatg gacgtccgat cgcattgctt gttcaggaag caagtcagta
     1261 cgcaagtacg atttatattc aggtcggaga gaaacacatt aatgcaaaaa gtattatggg
     1321 tatgatgagt ctgagtcttg ccgatgggga agagatcact gttgtgacag atggagaaga
     1381 cgagcagcga gcggctgaag gtataaaaac attttttgca aaaggcagaa aatagagtgg
     1441 atggataaaa cgaataaaag gaggagggct gtacatctgt gtgcagtcct ctcttttcac
     1501 gagatggaaa taacaaagta taggggcggt gacgcagtat gaaaaaaatg ttatttatct
     1561 ataatccgaa tgccggtacg ggaaaattaa aacctaaact tgcggatgtt ttggatattt
     1621 ttacgaagag cggatatgaa gtgactgttt atccgacaca gaaaagatat gatgcggctg
     1681 aaaaaataca agaaagcggg agtcagtatg atctgatcgt atgcagcggc ggagacggta
     1741 ctttagatga ggcggttaca ggaatgcagc tttgtaactg taaagttccg ctgggatata
     1801 tcccggcggg gacgacgaat gattttgcga caagtctcgg catcccgaaa gatattcttg
     1861 aggcggcgga gacggcggta aacggaacgg tcttcccgtg tgatgtaggg atgttcgggg
     1921 aagattattt tgcatacatc gcagcgttcg gactgtttac ggacgtgtct tatgccacgg
     1981 atcagaatgt gaaaaatgtt cttgggcata tggcgtatat tttggaaggc gcgaaaagag
     2041 tgtttaatat tccgtcttat aagattaaga tcacgcatga cggcgaagtg atagaggatg
     2101 agtttatatt cggaatggtg acaaattccc gatctgtcgg tggattcaag gggattatcg
     2161 gtccggagat cgtatttgac gacggagtat ttgaagttac gctgatcaag acaccgaaaa
     2221 acgcaataga attgaatgaa cttctcggcg cgatcgtgat gaaacagtta gattcaaaac
     2281 ggatgtattc gtttaagacg ggagaaatta agtttgagtc agaggaagaa atcccgtgga
     2341 cgttagacgg agaatttggc ggggcgcaca aagaaattac cgttaaagat aagaagcaag
     2401 ccgtgcagat caaagttgac tcagaggtgg cggcaggttt atcagtggat tccgaaaaag
     2461 taaaaaagta aaatgtaaaa aataaatcaa aaaaattaaa aaaatacttg catatagtaa
     2521 aaacatatgt tatatttata ttcgctgtga ggaacaccaa acagcgaata taaataaggc
     2581 tccgtggtca agcggtcaag acgctagcct ctcacgctgg aatcaggggt tcgattcccc
     2641 tcggagtcac ttttttaaag tgctgaaagc cttgaaaata tgcgggtttt cagcattttt
     2701 tattttttga aaaaacagaa ggtaatcaaa aaggtaatca aaagctaaaa gaaaggaaaa
     2761 tggttatggt gttcttttag ttttccttta agtaaaaagt atattgaata ttaagcgaac
     2821 gctgtgttag tgcgttcttt ttttatgcga aaatttaagc ataaggaggt atattttatg
     2881 cttaccgatg aatttttaga aagagttttt gcgaacaaag aaatgcagaa aatgccgatc
     2941 ggttgtcaat cgaccgctgt tcatgcattt caaaaagttt tagaagatat gaaggaggaa
     3001 aatccttatg cagacttatc cgcaatatta tcctcagatg aataattatg gtcaacagta
     3061 caatccgcaa caaccttata tggataggtt ggcaggttta cagcaatatc aacagacgtt
     3121 acaacaaccg caaatggcag ggatacaaat gtccttacca aatcagccta tcggattgaa
     3181 tgggaaagtg gtagatacta tagaacaaat cacagcgaat gatgttccga tggatggaag
     3241 tgttgcaatc tttcccaaaa aagatatgtc ggagatttat ttaaagtcat ggacgccgaa
     3301 cggaacaatc gcaaccgtag tttatcatcc agtcatggaa gagtcaagca attctgcatc
     3361 agttcaagat aatttaaaaa taggattatc cgatgaagtg acagaaatat ttatgcagaa
     3421 gtttgacgaa ctcgcaaata agattgaaga actagaaaaa tctatgacta aaccaatgac
     3481 aaaaacaacg gtttctagga ctaaaaagga gagtgagaca taatgaatcc tttgacaatg
     3541 ataggacaaa tgataaaaaa tggaggaaat ccgcagcaga ttttccgaca gatgatgggg
     3601 aataatcctg caatgaataa tccaattatg aaaaatgcgt ttgaaatggc gcaaaaagga
     3661 gactcaa
//
LOCUS       DBMDXF010000184         3648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5852_length_3648_cov_1.535382, whole genome shotgun sequence.
ACCESSION   DBMDXF010000184 DBMDXF010000000
VERSION     DBMDXF010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_3648_cov_1.535382"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="ACZPD4_07260"
     CDS             <1..891
                     /locus_tag="ACZPD4_07260"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1378889.1"
                     /translation="RNEKGIHREEKDAEEIIDKALKLCDKYEKIFSRTLPESEIYALN
                     HGTLPMEDGYYLLSEECAALIEKGLYYSELSGGAFDITIEPVSSLWDFTSGEKIVPDA
                     ETIAEKQACVGYEDVELKGNKLRFKKEGMGIELGAVAKGYIADKVKEYLVSEGVKSAV
                     IDLGGNVLCVGEKPDGKPFKIGIQKPFADRSETVSAVEIAGTSVVSSGIYERYFEKDG
                     KLYHHILDPATGYPYENGLVSVTVLSEESADGDGLSTACFALGLEKGLELIEQTPDTE
                     AMFITEDGEMHFSKGFVYCG"
     gene            1174..3060
                     /locus_tag="ACZPD4_07265"
                     /pseudo
     CDS             1174..3060
                     /locus_tag="ACZPD4_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            3118..3648
                     /locus_tag="ACZPD4_07270"
                     /pseudo
     CDS             3118..3648
                     /locus_tag="ACZPD4_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080593.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotidyltransferase"
BASE COUNT         1144 a          577 c          933 g          994 t
ORIGIN      
        1 agaaatgaaa agggaataca tagagaagaa aaggatgcgg aagagattat agataaagcg
       61 ttaaagcttt gtgacaaata tgaaaagata ttcagccgta cgcttcctga aagtgagatt
      121 tatgcgttga atcacggaac gcttccgatg gaagacggat attatctttt atccgaagag
      181 tgcgcggcgt tgatcgaaaa ggggttgtat tatagtgaac tttccggtgg agcgtttgat
      241 attacgatcg aacctgtttc ttctttatgg gattttactt caggggaaaa gatcgtgccg
      301 gatgcagaga caatcgcgga aaagcaggca tgtgtaggct atgaagatgt ggagcttaaa
      361 ggaaataaac tacgttttaa aaaagaggga atgggaattg agcttggagc tgtcgcaaaa
      421 ggatatattg cagataaagt gaaagaatat cttgtatcgg aaggtgtgaa aagcgcagtt
      481 atcgatcttg gaggaaatgt gttatgtgtc ggagaaaaac cggatgggaa gccgtttaaa
      541 atcggaatcc agaagccgtt tgccgatcgg agcgaaacag tgtcagccgt agaaattgca
      601 ggtacgtctg ttgtttcctc aggcatatat gaaagatatt ttgaaaaaga cgggaaactg
      661 tatcaccata tattagatcc ggcaaccgga tatccgtatg aaaacggact cgtgtccgta
      721 actgttttat ctgaagagtc ggcggacggt gacggattaa gtacggcatg ttttgcgcta
      781 ggattagaaa aaggattaga actgatcgaa cagacaccgg atacagaagc gatgtttata
      841 acagaggatg gagaaatgca ttttagcaaa ggttttgttt attgtggcta acgctactgt
      901 ttcacttata accgggcgct catctcatac agatgtccgc ctgcataaaa gaaaaagaga
      961 tatgaaggag agaaaaaaat gagtatatgt gatttaaagt cttatgaaat tgtacaaagc
     1021 agagatttat ctgatctgag ttcaaaagga acgcttctgc gtcataagaa gagcggagca
     1081 agagttcttc ttatggaaaa tgatgatgaa aataaagtgt ttgccatcgg ttttcgtaca
     1141 ccgccgtcag acagtacagg agtacctcat attatggagc attctgtatt gtgcggatca
     1201 agagagtttc cggtaaaaga tccttttgta gagcttgtaa aaggatcgct taatacattt
     1261 ttaaatgcga tgacttatcc tgacaagaca gtttatcctg tagcaagctg caatgataaa
     1321 gactttcaga atcttatgca tgtctacatg gacgcggtat tttatccgaa tatttacgag
     1381 caggataaaa cgtttcgtca ggaaggatgg agttataaac tggatgcacc ggatgaggaa
     1441 ttgaagttgt cgggagttgt atacaatgag atgaaaggtg cgttttcttc acctgaaggg
     1501 gtgcttgacc gggtgatttt gaattctctg tttccggata catcgtatgc aaatgaatcc
     1561 ggcggcgatc cggaagtgat ccctgatctg acgtatgaac agtttcttga tttccacagg
     1621 aaatattatc atccgtcaaa cagctatatt tatctgtatg gcgatatgga tatggaagaa
     1681 aaactgaact ggcttgatga aaagtatttg tctgattttg atgagattga ggttgattcg
     1741 gagattaagt atcagaagcc gttttcacaa atgcaggaga ttgttcagga atattctata
     1801 gcaagcgatg aaagcgaagc ggataacact tatctttctt ataataaagt gatcggaact
     1861 tccttagatg aaaagctcta tcttgcattt cagattttag attatgcgct tttgtctgct
     1921 ccgggagcgc cgctgaaaaa agcgcttctt gatgcgggaa tcggaaaaga tattatggga
     1981 tcatatgata atggagttta tcagccgatt ttttctgtta tttctaaaaa tgcaaatatg
     2041 gagcagaagg aagcgtttgt ggaagtgatt gaaaatacgc tgcgaaatat tgcagaaggc
     2101 ggaattgata aaaaagcatt gcaggctgga ctgaattatt atgagttccg tttcagagag
     2161 gcggacttcg gaagttatcc gagaggattg atgtacggac ttcagttgtt cgatagctgg
     2221 ctttatgatg aagaaaaacc gtttatccat atggaggcga ttccgacatt tgagtttttg
     2281 aaaagtcaga tcgaaacagg ttattttgag cagttgatcc gagattatct tttggagaat
     2341 ccgcacgggg cgatcgtaat catccgaccg gagaagggaa ggactgcgag aatggatcgg
     2401 gagcttgcag agaaactgca ggcgtataaa gaaagtctgt ccgaagaaga gattgagaaa
     2461 cttgtgcagg atacaaaaga tctggaagcg tatcaggaag aagaatctgc gccggaagat
     2521 ctggcgaaga ttccggtgct tagaagagaa gatatttccc cggaaatcgc gcctgtctat
     2581 aatacagaga tggagatcga ctcagtgaaa acgatctatc ataatgttga gacaaacgga
     2641 atcgggtatg taacgcttct gttcgatctt tctgcggtca aggaagaaga tcttccgtat
     2701 gtcggcattt tgcaaagtgt cctcggcatt atcgatacag agaattacga gtatggagag
     2761 ttgtttaatg agatcaatat ccatacaggc ggaatcggaa cgtcgcttga attatatgca
     2821 gatgcgcaga aagtaaagga aaaggaattc aaagcgacat ttgaaatgaa aggaaaatca
     2881 ctgtatccga agatggacgt tcttttctct atgatgcggg aaatactgac ttgttctaag
     2941 cttgacgatg agaaacgctt aaaagagatt cttgcgatgc tcaaatcacg ccttagtatg
     3001 tcgttcttgt catcgggaca tacaactgcc gctttgcgtg cgttgtcata tacatcgcct
     3061 cttgcaaaat ttaaagacga tacagacgga atcgaatttt acgaagtagt aaaagaaatc
     3121 gaagaaaact ttgacgacca taaagaagaa ttgatctgtc ggttaaaagc aatttcaaaa
     3181 cagattatgc cggtttatga taaaccgatg atttattatc cactgtcaac actgatgctg
     3241 gcaggaatcc gtgaggtgtt gattatttct acacctagag atttaccggt atttgaggaa
     3301 ttattaggag acggcagcca gttgggaatg cgtttttagt atgctgttca ggaagagcca
     3361 agaggactgg cagatgcatt tattattggc gaggagttta ttggaaagga tgcagttgca
     3421 cttgttttgg gagataatat cttctatgga cagagtttct ccaaggtact gagaagtgcc
     3481 gcagagcgta cagagaacga aggcggagca acaatcttcg gttattatgt aaaagacccg
     3541 agagaatacg gtgtggtaga gtttgacgag gatggacggg ctatttcgat tgaagagaag
     3601 ccgcgccatc cgaaaagcaa ttacataatc cccggcattt acttttat
//
LOCUS       DBMDXF010000185         3638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5867_length_3638_cov_2.141080, whole genome shotgun sequence.
ACCESSION   DBMDXF010000185 DBMDXF010000000
VERSION     DBMDXF010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5867_length_3638_cov_2.141080"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="ACZPD4_07275"
     CDS             complement(<1..173)
                     /locus_tag="ACZPD4_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR1378890.1"
                     /translation="MKMENERLCIEISEHGAEVTRIYDKKKETELLWEGNPTYWKRHS
                     PILFPNVGKTYKN"
     gene            complement(295..1404)
                     /gene="tig"
                     /locus_tag="ACZPD4_07280"
     CDS             complement(295..1404)
                     /gene="tig"
                     /locus_tag="ACZPD4_07280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1378891.1"
                     /translation="MKKRIVSMMLAAMVALSVVGCGSKTLSNDYVTVKQYKGLEVAQV
                     EKVEVTDEQVEQSVQANLNAAAEKEPIKDRAAEKGDWVNIDYTGYIDDVAFEGGTATG
                     SDLELGSGSFIGAEGGYAGFEDQIIGHSTGEEFDIEVKFSDSYPGTDVAGKVARFHIV
                     LNEIYKQNIPELTDEWVASNSKTAKTADEYRKEVRKQIESSAETANETTLKSEIQEAL
                     LEEIDVKKYPKDAVEEQKKQMTDYYTQMAGLYGIELSEFITTYLQMTEDEFNSKVKES
                     AQQTAAFDEAIKLIAEKQKLEPSEKEYKEKFKEYAEQAGADMDAYVEQVGEDVLKAAI
                     LRDAVLDYLVDNCVQVENSDSDADSNSNNKEDSSK"
     gene            complement(1547..3022)
                     /gene="cls"
                     /locus_tag="ACZPD4_07285"
     CDS             complement(1547..3022)
                     /gene="cls"
                     /locus_tag="ACZPD4_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1378892.1"
                     /translation="MAEMIWNAAVSVYNFIMDNIVLINIFLALVIVFFQRRSPQTVWT
                     WLLLLYFIPILGFVMYLIIGQDFHKSRMFKAKEIEGELKYAVRRQEETIYYKRLRMAN
                     PEMSRFKDLILYNLEAGEAVLTDNNDIRVYTDGKEKFRALIEEMKQAKRYIHMQYYII
                     RNDELWQEVEKVLIQKAREGVEVRVLFDSMGCRTMKNRDWERLEKEGIQVAEFFPALL
                     GQLQLRVNYRNHRKIVVIDGRVGFVGGFNVGREYLGRDKKFGYWRDTHLCIEGAAVTS
                     LAVRFVLDWNYAAKENLFLEDSLFEIPQYTRNGRDPVQIISSGPDSKTKMIHDNYLRL
                     IHRAQDHVYIQTPYFIPDDSILDALKIAAKSGVDVRIMIPCKPDHPFVYWATYSYIGE
                     MVAAGAKCYVYNNGFLHAKTLSIDGMVACVGTANMDFRSFGLNFEVNAVIYSERTVQR
                     LERAFENDMTLCTQVTRKVYDERGLVIKAKEQFSRLLSPLL"
     gene            3192..3623
                     /locus_tag="ACZPD4_07290"
     CDS             3192..3623
                     /locus_tag="ACZPD4_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378893.1"
                     /translation="MSTFTIVMIVILVILIGVLVGLYIFGKKAEKKQAEQQEQMAAAA
                     QVVSMLIIDKKKLRLKEAGLPQMVVDNTPKYLRRTKVPVVKAKIGAKIMTLMCENKVF
                     EVLPVKKEVKAVVSGLYITEIKSVRGGSIEQPEKKKGFFKK"
BASE COUNT          931 a          924 c          637 g         1146 t
ORIGIN      
        1 ttatttttat aggtttttcc tacgttcggg aaaaggatcg gggaatgtct tttccaatac
       61 gtgggatttc cctcccacag aagttccgtc tctttctttt tatcatatat acgggtaacc
      121 tcagccccat gctctgaaat ctcaatacag agccgttcgt tttccatttt cattgcctct
      181 gtctcctttt ccctgaatac tttcttttgc ttttacactg cccgatccac atccggttca
      241 ggcagcgcat gtcctgcctt cacatatctc atttaccggc atccggcaca gatcttattt
      301 actgctgtct tccttgttat ttgaattact gtcggcatca ctgtctgaat tttctacctg
      361 aacacaatta tccacaaggt aatcaagcac agcgtctctt aaaatcgcag ctttcagcac
      421 atcttctcca acctgttcta cataagcatc catatcggct cccgcctgct ctgcatattc
      481 tttgaatttt tctttgtatt ctttttctga cggctcaagt ttctgctttt ccgcgatcag
      541 tttaattgcc tcgtcaaacg ccgctgtctg ctgtgcgctt tcttttactt ttgaattaaa
      601 ctcatcttct gtcatctgca gatatgtcgt gatgaactca gacaattcaa ttccgtataa
      661 tcctgccatc tgtgtatagt aatctgtcat ctgctttttc tgttcttcca cagcgtcttt
      721 cggatatttt ttcacatcga tctcttcgag aagcgcttcc tggatctctg attttaatgt
      781 tgtctcattt gctgtctccg cgctgctttc aatctgttta cggacttctt tgcggtattc
      841 gtccgctgtt tttgccgtct tgctgttgct tgccacccac tcgtctgtca attccggaat
      901 gttttgtttg taaatttcat tcaatacgat gtggaaacgt gcaacttttc ctgccacatc
      961 tgttcccgga taagaatccg aaaacttcac ttctatatcg aattcttctc ctgtactgtg
     1021 tccgatgatc tgatcttcaa atccggcata accgccctct gctccgataa agcttccgct
     1081 tccgagctcc agatcggatc ccgtcgctgt tcccccttca aatgccacat catcgatata
     1141 tcctgtatag tcaatgttta cccaatcgcc tttttccgcc gcacgatctt tgatcggctc
     1201 tttttctgcc gccgcattta agtttgcctg aacagactgc tcaacctgct catctgtcac
     1261 ttcaaccttt tctacctgtg caacttcaag acctttatac tgctttaccg ttacatagtc
     1321 gtttgacaat gttttacttc cgcatcctac aacggaaaga gccaccattg ccgcaagcat
     1381 cattgataca attctttttt tcataataat ctcctttttt gtccgcgttt tatacgcgtc
     1441 ttttatttat atttattttt actgcatgca tatttgccgt acgttttttt tgattttcac
     1501 gtacgagttc catctatata cgcctgatct ttcctttatc atccttttac aaaagcgggg
     1561 aaagcaagcg cgaaaactgt tctttcgctt ttatgacaag tcctctttca tcatatactt
     1621 tccttgtcac ctgcgtacac agcgtcatat cgttttcaaa cgcacgttcc aatctttgta
     1681 ctgtacgctc gctgtaaatc actgcgttca cctcaaaatt caacccaaaa ctccgaaagt
     1741 ccatatttgc cgttccgaca catgcgacca tgccgtcgat actcaatgtc ttagcgtgaa
     1801 gaaatccgtt gttgtacaca tagcattttg ctccggcggc aaccatctct ccgatataag
     1861 agtaagtcgc ccagtaaaca aacggatgat ccggcttgca cggtatcatg atccgcacat
     1921 caacgcctga ttttgcggcg attttcaaag cgtccaagat cgaatcgtcc ggtataaagt
     1981 acggcgtctg gatatagaca tgatcctgtg ccctatgaat aagtcgtaag taattgtcgt
     2041 ggatcatctt cgttttcgag tccggtccgc ttgatatgat ctgcaccgga tctcttccgt
     2101 ttctcgtata ttgcggaatt tcaaacaatg aatcttctaa aaacaagttt tctttcgccg
     2161 cataattcca atcgagaaca aaccggactg ccagcgacgt taccgccgcg ccttcgatac
     2221 acagatgggt atcccgccaa taaccgaatt tcttatcccg tccgagatac tctcttccga
     2281 cgttaaatcc tcctacaaaa ccgactcttc cgtcaataac aacgattttt ctgtgattcc
     2341 ggtaattcac acgaagctgc agctgcccaa gcagcgccgg gaagaattcc gcaacttgaa
     2401 ttccttcttt ttcgaggcgc tcccagtcac ggtttttcat cgttctgcat cccatactgt
     2461 caaaaaggac acgaacttcc acgccttctc tcgctttttg aataagtacc ttctctactt
     2521 cctgccacaa ttcatcgttg cggataatgt aatactgcat gtggatatat ctcttcgcct
     2581 gcttcatctc ttctataagc gcacggaact tctcttttcc gtccgtataa acgcgaatat
     2641 cgttattatc tgtcagaacc gcttccccgg cttccagatt ataaagaatc agatctttaa
     2701 accttgacat ctccggattc gccatgcgaa gccgtttata ataaatcgtc tcctcctgcc
     2761 gccggactgc atattttagt tcaccttcga tctcttttgc tttaaacatc ctgctcttgt
     2821 ggaaatcctg tccgatgatc aaatacataa caaatccgag gatcggtata aaataaagca
     2881 aaagcagcca tgtccaaaca gtctgcgggg atctgcgctg aaaaaagacg atgaccagcg
     2941 caaggaaaat attgatcaga acaatattat ccataataaa attatagaca ctcaccgctg
     3001 cgttccaaat catttctgcc ataaataata taactccttt ttatgtactt tcatgtacta
     3061 taacagtata ctcgttcacc tactcaaaag taaacccctt tttacttgca ctacacaaaa
     3121 aacaatgtta caatgatatt tgcgtaaatg cggaaaataa cattatacag taggatttag
     3181 gaggagttgt catgagtaca ttcaccattg tgatgatcgt cattctcgtg atattgatcg
     3241 gggtgctcgt cggtctttac attttcggaa agaaggccga gaaaaaacag gcggaacagc
     3301 aggagcagat ggcagcggcg gcacaagttg tcagcatgct catcatcgat aaaaagaaac
     3361 taagattaaa agaagcggga cttccgcaga tggtcgtaga caatacgccg aagtatcttc
     3421 gccgtacaaa agttccggtc gtaaaagcaa agatcggcgc aaagatcatg actcttatgt
     3481 gcgagaacaa agtgtttgaa gttcttccgg tgaaaaaaga agtcaaagcg gttgtaagcg
     3541 gactctacat aacagagatc aaaagcgtac gcggcggttc tatcgaacag cctgaaaaga
     3601 aaaaaggatt tttcaaaaaa taaaaaaaaa aaaaaaaa
//
LOCUS       DBMDXF010000186         3626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5889_length_3626_cov_1.606241, whole genome shotgun sequence.
ACCESSION   DBMDXF010000186 DBMDXF010000000
VERSION     DBMDXF010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5889_length_3626_cov_1.606241"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="ACZPD4_07295"
     CDS             complement(<1..384)
                     /locus_tag="ACZPD4_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1378894.1"
                     /translation="MGQNETTTNELLLRIKEKYKKMSKGQRRLADYVCNDYDKAVFLT
                     AAKLGEIVGVSESTVVRFATQLGYKGYPGFQKALEELVRNKLNSIQRMEVTYGRISQS
                     EILETVLQSDIDKIKMTLADIDHKAF"
     gene            complement(475..2274)
                     /locus_tag="ACZPD4_07300"
     CDS             complement(475..2274)
                     /locus_tag="ACZPD4_07300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR1378895.1"
                     /translation="MKYSGSISDRIKALRAEMRREKIDLYIIPSTDYHNSEYIGEYFK
                     ERQYMTGFTGSAGTVVFTEEKAGLWTDGRYFIQAEQELQGSEIILFKAGEPGCPEIEE
                     FIRTELPEGGKIGFDGRTIRVEQGKEFEKIAEEKCGALSYLSDLVDVVWKDRPPLPTE
                     KAFFLDEFYSGETAASKLERVRCKMDESGADVHLLSSLDDIAWLLNIRGNDILCCPLV
                     LAYLIIYKDHVELFADEEKFSDDMKREFAKNHVALRPYTEIENAVGKLSGRKKMLIDP
                     ERLSYALYKLIPDETEMIEKENPEIIMKSVKNDIETEHIRRAHLKDAAAHTKFIYWLK
                     ENIGKTEITERSASARLEEFRKEQDGYLGPSFEPISAYYEHGAIVHYSASKESDARLE
                     KGHFLLTDTGGHYKDGSTDITRTVALGEVSYQEKEDFTLVLRSMLRLMNAVFLEGCSG
                     ANLDCLAREVFWKERLNFNHGTGHGVGYLLNIHEPPINFRWKEGKNAAPALQKNMIIT
                     DEPGIYRAGRHGVRIENDLLVVEDTQNEFGKFLKFEPLTYVPIDLDAILPEKMSDEEK
                     KMLNEYHAAVYEKVGMYLEENEREWLKRYTRPI"
     gene            complement(2398..>3626)
                     /locus_tag="ACZPD4_07305"
     CDS             complement(2398..>3626)
                     /locus_tag="ACZPD4_07305"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HIR1378896.1"
                     /translation="KEDPWERLRKFRDGFKNTKLQMLFRGQNILGYRPYADDVVEYFV
                     QKSAANGIDIIRIFDCLNDIRNLKTAVTAANKEKAHAQVAMSYTLGDAYTLDYWVDLA
                     KRIEDMGANSICVKDMAGLLVPYEATELVTALKEAVNIPIEMHTHYTSGVASMTYMKS
                     VEAGADIIDTAMSPFALGTSQPATEVMVETFKGTPYDTGLDQKLLSEIADYFRPIRDE
                     ALESGLLNPKNLGVNIKTLLYQVPGGMLSNLTSQLKEQGAEEKFYEVLEEVPRVRKDL
                     GEPPLVTPSSQIVGTQAVFNVLMGERYKMATKETKDILAGKYGATVKPVDAQLQKKIL
                     GENAEIITCRPADLIPNELDTLRKECEQWIQQDEDVLTYALFPQVAVDFFKYRQAQQT
                     KVDAAVADTKNGAYPV"
BASE COUNT          939 a          907 c          579 g         1201 t
ORIGIN      
        1 aaatgcttta tggtcgatat ctgcaagagt catcttaatc ttatctatat cggattgaag
       61 aacagtttcc agtatttcac tttggctgat tcttccatac gtcacttcca tcctctggat
      121 cgaattcagc ttattacgga caagttcttc taatgccttt tgaaatcccg gatatccttt
      181 atagccaagc tgtgtagcga atcgcactac agttgactca ctgaccccga cgatctctcc
      241 taatttggca gccgtcaaaa aaaccgcttt gtcgtaatca ttgcatacat aatctgcaag
      301 acgccgctgt cctttactca tttttttata tttttcttta attcttaata aaagttcatt
      361 tgtcgttgtt tcattttgtc ccatttttgt ttcctacttt ttctcaatta tcaccaactg
      421 attatatctt attttttctt tactgtcatt gtccgatttg cagatgtgca taatttatat
      481 cggtcttgta tatcgcttca gccactctcg ctcgttctcc tctaaataca ttccgacttt
      541 ttcataaacg gcagcatgat attcgttcag catctttttt tcttcatcac tcattttttc
      601 agggagaatt gcatcaagat caatcggaac ataggtaagc ggctcaaatt tcaaaaactt
      661 tccgaactcg ttctgcgtat cttctacgac aagaagatca ttttcaattc tgacgccatg
      721 tcggcctgct ctgtaaatcc ccggttcatc tgtaattatc atattttttt gaagagccgg
      781 cgcagcattt ttgccttcct tccatctgaa attaatcgga ggctcgtgaa tattcaaaag
      841 atatccaacc ccatgacccg tgccatgatt aaaattcagc ctttccttcc aaaacacttc
      901 tcttgcaaga cagtctaaat tcgcaccgct gcacccttcc aaaaacacag cgttcataag
      961 tctgagcatc gaacgcaaaa caagagtgaa atcttctttt tcctgataac tcacttcacc
     1021 taaagccacg gttcttgtaa tatcagtcga tccatcctta taatgtcctc cggtatctgt
     1081 taaaagaaaa tgcccctttt ctaatcgtgc gtcgctttcc ttcgatgcac tgtaatggac
     1141 aatcgctcca tgttcgtagt atgcgctgat tggttcaaaa cttggtccta aatatccatc
     1201 ctgctctttt ctaaactctt ctaaacgagc ggaagctgac cgttcggtaa tctctgtttt
     1261 cccgatattt tctttcagcc aatatataaa ttttgtatgt gcagccgcat cctttaaatg
     1321 cgccctgcga atatgctctg tttcaatatc attttttaca gatttcatta tgatttccgg
     1381 attctctttc tctatcattt cagtttcatc cggtatcaat ttatataaag catagctaag
     1441 acgctcggga tctatcagca ttttcttcct gccgcttagt tttccgaccg cattttcgat
     1501 ttctgtatat ggtcttagag caacatgatt tttcgcaaat tcccgcttca tatcatcgga
     1561 gaacttttct tcatctgcaa acaattcaac atgatcctta tagattatta aatatgccaa
     1621 aacaagagga caacacaaga tatcgtttcc tctgatgttt aatagccacg caatatcatc
     1681 aagacttgat aacagatgta catcagctcc tgattcatcc atcttacacc ttactctttc
     1741 caacttagaa gctgctgttt ctcccgagta aaattcatcc aaaaagaacg ctttttccgt
     1801 cggcaaaggc ggtctgtctt tccagacaac atcgacaaga tccgaaagat aagacagcgc
     1861 tccgcatttt tcttcggcaa tcttttcaaa ttcttttcct tgttctaccc gaatcgtacg
     1921 gccatcaaat ccgatctttc cgccttccgg caattctgtc cgtataaatt cttcgatttc
     1981 cgggcagccc ggctcccctg ctttaaaaag gatgatctca ctgccctgca gttcttgttc
     2041 tgcctgtata aaatagcgcc cgtctgtcca aagccccgct ttctcctctg taaacaccac
     2101 agttccagca gagcctgtaa atccggtcat atattgacgt tccttaaaat attcacctat
     2161 atattcactg ttatgataat ctgttgacgg aataatataa agatctatct tttcccgtct
     2221 catctcggct ctaagagcct taattctatc tgaaatgctt cccgaatatt tcattcaaga
     2281 cctcccgctt ctcaaaattc ctttttatga taccactaaa cgaaaaagag tacaaataaa
     2341 acaggatcga tctcctgaat aaattcaatc gttcgatcct gtcactattc cttttatcta
     2401 taccggatat gctccgttct tagtatctgc tactgcagcg tcaacttttg tctgctgtgc
     2461 ctgtctgtat ttaaagaaat caacggcaac ctgcgggaat aatgcatacg taagaacatc
     2521 ttcatcctgc tgtatccact gctcgcactc tttgcgaagt gtatcgagct cattcgggat
     2581 caaatctgcc ggacgacatg tgatgatctc tgcattctca cctaaaatct ttttctgaag
     2641 ctgagcgtca accggtttta cagtcgcgcc atattttcct gcaagaatat cttttgtctc
     2701 ttttgtcgcc atcttatatc tctcacccat cagaacatta aatacagcct gtgttccgac
     2761 aatctgagaa gacggagtaa caagcggcgg ctcgccaaga tctttacgca cgcgtggtac
     2821 ttcctcaagc acttcataga atttttcttc cgctccctgt tcttttaact gagatgtcag
     2881 attagaaagc attcctcccg gaacctgata taataaagtc ttaatattaa ctccgaggtt
     2941 tttcggatta agcagtccgc tttcaagcgc ttcatctctg atcggacgga aataatccgc
     3001 aatctcagat aacagtttct gatctaatcc agtatcatac ggcgtacctt tgaacgtttc
     3061 caccatcact tccgttgccg gctgcgacgt tccgagagca aacggagaca ttgcggtgtc
     3121 aatgatatca gctcctgcct ctacagattt catataagtc atcgaagcaa ctcccgatgt
     3181 ataatgtgta tgcatttcaa tcgggatatt tactgcctct ttcagcgctg tgacaagttc
     3241 tgtcgcttca tacggaacaa gaagtcctgc catatcttta acacaaatag aattcgctcc
     3301 catatcttcg attctctttg caagatccac ccagtaatca agagtatacg catcgccaag
     3361 tgtataagac atcgctacct gtgcatgtgc tttttcttta tttgcggccg tcactgctgt
     3421 cttcaagttt ctgatatcat tcagacaatc aaaaattcgg ataatatcaa ttccatttgc
     3481 cgctgatttt tgtacaaagt attctactac atcatctgcg tacgggcgat accccagaat
     3541 attttgtccg cggaacagca tctgaagttt tgtattttta aatccgtcac ggaatttacg
     3601 aagcctctcc catggatctt ctttca
//
LOCUS       DBMDXF010000187         3594 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_5940_length_3594_cov_1.524478, whole genome shotgun sequence.
ACCESSION   DBMDXF010000187 DBMDXF010000000
VERSION     DBMDXF010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_3594_cov_1.524478"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(10..192)
                     /locus_tag="ACZPD4_07310"
     CDS             complement(10..192)
                     /locus_tag="ACZPD4_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HIR1378897.1"
                     /translation="MSAGLPRDPVMLLSVVNTKLRDYYSTLDVLCEDMQVDKQELIGK
                     LEMIDYTYDAGSNQFV"
     gene            complement(162..746)
                     /locus_tag="ACZPD4_07315"
     CDS             complement(162..746)
                     /locus_tag="ACZPD4_07315"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1378898.1"
                     /translation="METKRDIRKMMTALRAAMSEKERAEAGRIIAARLFSSKVYEDAG
                     TVCCYASFGSEVPTEEIIEESLRRGKCAAVPKVTGKQKMKFALIHSMADLKAGFHGIP
                     EPESWCREIPKCGSKLLVIVPGVVFDRAGNRIGYGGGYYDSYLKQADCIKVGVAFDFQ
                     CVEQIVPEVHDVPVDYIITEKEMITCRQACQEIR"
     gene            complement(724..945)
                     /locus_tag="ACZPD4_07320"
     CDS             complement(724..945)
                     /locus_tag="ACZPD4_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR1378899.1"
                     /translation="MDQQKIARINELYRKSKAEGLTEAEKKEQKILRTEYMDSVKRNL
                     RSQLNNIDIEEKDGSVINLGEKYGNKKGH"
     gene            complement(1081..1479)
                     /locus_tag="ACZPD4_07325"
     CDS             complement(1081..1479)
                     /locus_tag="ACZPD4_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378900.1"
                     /translation="MNSKYRIGFLCAGAAFFLFITLAYKMTYDYTMDKYMEKVSERNE
                     DVQKADGIGKTESIAAEGEAVKGGAKKGFYLKELHGFVVVYLEDKKTIYEFTEIPLSD
                     LPEEVRQEIAQGKQIATAEELYAFLENYSS"
     gene            complement(1540..3225)
                     /locus_tag="ACZPD4_07330"
     CDS             complement(1540..3225)
                     /locus_tag="ACZPD4_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIR1378901.1"
                     /translation="MGDTQANQRKKTRGEDYIAQGSILAVAVVITKIIGVVYRIPLTN
                     ILGDEGNGFYGYAYQVYAIALMLSSLSLPTAVSKLVSVRMAVGQRRNAFRVFLCSLIF
                     AVSVGIIISLAIFLGAGAISEHMMKAPFSVYALKVLSPGLLIVAVMAVLRGYFQGLGT
                     MVPTAVSQIIEQIINAVVSIVGASVLFGMGSRAGEKAGEELLGPAYGAAGGTLGTVIG
                     AAAGLLFLLFVLWIFKGGIRRQIQRDHTKGKESYSHILKILVMTAIPVIFSTAIYNIN
                     QILDLTVFNHIMDAQGFTESEYMALQGIYTGKYDPLINVPMSIPYALSTSIVPALTTV
                     VMARNRKKTHYQIDQTLRLTTIITLPSCVGFLVLASPLMVLLYNDAGKVPAQLLMLGS
                     VVIILYGWSTITNAVLQGLNYLSSPAKNAAAALVVHLGALVLMLTVFKLNVYALVGSN
                     IVFAVIMCVLNQRKIRQVCGFRINIRRTFVKPLIASAIMGVITYAVHFGLHRLIGGRV
                     IPTVLAIIVAVIVYGVLILKLGALSEKDIQALPMGTRILRGCQALKLMPPSEE"
     gene            complement(3268..>3594)
                     /gene="ybeY"
                     /locus_tag="ACZPD4_07335"
     CDS             complement(3268..>3594)
                     /gene="ybeY"
                     /locus_tag="ACZPD4_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1378902.1"
                     /translation="QRQIDRATDVLSFPMADYETPGDFSHLDEDAGLFHPDTGELMLG
                     DIVISVDKVFEQAEEYGHSLLREYAFLIAHSMLHLFGYDHMEEVERKEMEMHQKKIME
                     LVNITR"
BASE COUNT          978 a          930 c          563 g         1123 t
ORIGIN      
        1 ttattccact cacacaaact gattactgcc tgcatcatat gtataatcga tcatctctaa
       61 tttaccgatc aattcttgtt tatcaacctg catatcttca cacaacacat ccagcgtcga
      121 ataataatct cttaattttg tatttacaac acttaagagc atcaccggat ctcttggcaa
      181 gcctgccgac atgtgatcat ctccttttcc gtaataatat aatctacagg tacgtcatgc
      241 acttccggaa caatctgctc cacacattga aaatcaaatg ccactccgac ttttatgcaa
      301 tccgcctgct tcaaatatga gtcataatat cctccgccat agccgattcg gtttcccgct
      361 ctgtcaaaca caactccggg cacgattaca agcagcttgc ttccacactt tggaatttcc
      421 cgacaccatg attccggttc cggtatccca tgaaatcccg cttttaaatc tgccatactg
      481 tgaatcaggg caaacttcat tttttgcttt ccggtcactt tcggaaccgc agcacatttt
      541 cctcttctaa gcgattcttc tataatttct tccgtaggga cttcacttcc gaaagacgca
      601 tagcagcata ctgtgccggc gtcttcatac acctttgaag aaaaaaggcg cgccgcgatg
      661 atgcgccccg cctctgctct ttctttttca gacattgcgg cacgaagcgc cgtcatcatt
      721 tttctaatgt ccctttttgt ttccatattt ttctccgaga ttgatcacac ttccgtcctt
      781 ttcttcaatg tcgatattat tgagctgact tcttaaattt ctcttcaccg agtccatata
      841 ttctgtcctt aaaatcttct gttctttttt ctctgcttca gtcaggcctt ccgctttcga
      901 ttttctgtat aattcattga tccttgctat cttctgttga tccattataa tccctttcac
      961 tttttatctt ctatccataa taacttctta ttttacaggt ctgtattata cactatttct
     1021 ttgattacgt ccatcgccat tttgcggctt ttatcattcc ggccttttcc gttttgcttt
     1081 tcaactcgaa taattttcta aaaaagcata taattcttct gcagttgcaa tttgctttcc
     1141 ctgtgcaatt tcctggcgca cttcttccgg aagatctgac agaggtattt cggtaaattc
     1201 ataaatcgtt tttttatctt caagataaac gacgacaaat ccgtgaagtt ccttaagata
     1261 aaaccccttt tttgctccgc cttttactgc ctctccttcg gccgcaatac tttccgtttt
     1321 tccgatgccg tctgcctttt gtacatcttc gtttctttca ctcacctttt ccatatactt
     1381 gtccatcgta taatcatatg tcattttata tgcaagcgtt ataaacaaga aaaatgccgc
     1441 gcccgcacac aaaaagccga tcctgtattt tgagttcata taatctaccc cttttctata
     1501 cagtatcagc ttttttcttt tatttattca cttatttttt tattcttcgc ttggaggcat
     1561 caatttcagc gcctgacatc ctctcaatat ccgtgttccc atcggaagcg cctgaatatc
     1621 tttctccgaa agagctccta attttaaaat aaggactcca taaacaataa ctgccacgat
     1681 aattgccaaa acagtcggga taactcttcc tccgatcaga cggtgaagtc cgaaatgaac
     1741 agcatacgtt ataacaccca taatcgccga agcgatcaac ggctttacaa atgttcttcg
     1801 tatattgatt ctgaatccgc atacctgacg tatttttctt tgatttaaaa cacacataat
     1861 aacagcaaat acaatgttgc ttcctacgag cgcatataca ttcaatttaa acactgtgag
     1921 cattaaaaca agcgctccca gatgaacgac caatgccgct gccgcgtttt ttgccggaga
     1981 agataaataa ttcaatcctt gcaaaactgc atttgtaatt gtagaccatc cgtacaaaat
     2041 aataactacg gaacctaaca ttaaaagctg cgccggcact tttcccgcat cattatatag
     2101 aagcaccata agcggagatg ccagtacaag aaatccaaca cagcttggaa gcgttatgat
     2161 cgttgttaaa cgaagcgtct gatctatctg ataatgtgtc tttttcctgt ttcttgccat
     2221 cacaaccgtt gtcagtgcag gaacgatcga tgtgctaagg gcataaggta ttgacatcgg
     2281 cacgttaata agcggatcat attttccggt ataaattccc tgaagagcca tatactcact
     2341 ttctgtaaaa ccttgcgcat ccataatatg attaaaaacg gtaagatcca atatctggtt
     2401 gatgttgtaa atcgccgtgc tgaaaataac gggaattgcc gtcataacga gaatcttcaa
     2461 aatatgagaa taactttctt ttccttttgt atgatccctt tgaatctgac ggcgtattcc
     2521 gcctttaaat atccaaagaa caaacaacaa gaacaaaagt cccgccgcgg ctccgattac
     2581 tgttccaagc gtacctcccg ccgctccata agcaggacct aacagctctt ctcccgcctt
     2641 ctctcccgca cgacttccca ttccgaaaag gacgcttgct ccgacaatac tgacaactgc
     2701 attgatgatc tgttcgataa tctgcgatac cgctgtcgga accatcgttc ccagtccctg
     2761 aaaatatcct cttaaaacag ccataacagc aacgatcaaa agccccggcg acagcacttt
     2821 aagtgcgtaa acactgaaag gcgctttcat catatgttcc gaaatcgctc ctgctccgag
     2881 aaagatcgca agagatataa taattcctac cgaaacagca aatataaggg aacataaaaa
     2941 tacgcggaac gcattgcgcc tctgtccgac agccatcctt accgacacaa gttttgatac
     3001 tgctgtcgga agacttaatg aagaaagcat caatgcaatc gcatatactt gataagcata
     3061 tccgtaaaaa ccgtttccct catcccctaa tatgtttgtc agcgggatac ggtacacaac
     3121 tccgataatc ttcgtgatca caaccgccac agccaggatc gacccttgag cgatgtaatc
     3181 ttctcccctt gttttttttc tttgatttgc ctgtgtatcc cccatagttg ttctcctttt
     3241 atccaatgat cccgaacctg aaaacattta tcttgtaata ttcacaagtt ccataatctt
     3301 tttttgatgc atctccattt cttttctttc cacttcttcc atatgatcgt aaccaaataa
     3361 atgaagcatg ctgtgcgcaa ttagaaatgc atattcccga agcagcgaat gtccgtattc
     3421 ttctgcctgt tcaaacactt tatctacaga gatcacaata tccccaagca tcagctctcc
     3481 tgtatcggga tgaaacagtc cggcatcttc atccaaatgc gaaaaatctc ccggtgtctc
     3541 ataatctgcc ataggaaatg acaaaacatc cgttgcccgg tcaatctgcc gctg
//
LOCUS       DBMDXF010000188         3540 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6033_length_3540_cov_2.032858, whole genome shotgun sequence.
ACCESSION   DBMDXF010000188 DBMDXF010000000
VERSION     DBMDXF010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6033_length_3540_cov_2.032858"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="ACZPD4_07340"
     CDS             complement(<1..1001)
                     /locus_tag="ACZPD4_07340"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR1378903.1"
                     /translation="MDKRPFVTKEKVEKITKIYPTPFHLYDEKGIRENVKALKEAFSW
                     NKGYKEYFAVKATPNPFLIDILKEYGCGCDCSSYTELMMSEALEITGEDIMFSSNDTP
                     AEEFIYADKLGAIINLDDITHIDFLEQAIGHVPETISCRFNPGGLFKISNDIMDNPGD
                     AKYGMTPEQILEAYKILKSKGAKEFGLHAFLASNTVTNDYYPMLAKVLFEQAVKLSKE
                     AGVHIKFINLSGGIGIPYKPDQEPNDIYAIGEGVRKVYEEVLVPAGMGDVAIYTELGR
                     YMMGPYGCLVTKAIHEKNTYKNYIGVDACAVNLMRPAMYGAYHHITVLGKEDEPCDH"
     gene            complement(1061..2497)
                     /gene="pyk"
                     /locus_tag="ACZPD4_07345"
     CDS             complement(1061..2497)
                     /gene="pyk"
                     /locus_tag="ACZPD4_07345"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR1378904.1"
                     /translation="MKKTKVVCTMGPNTNDRELLKKLIENGMDVARFNFSHGDHEEQK
                     SRMDLLKELRQELNTNTAILLDTKGPEIRTGVLKGGKRIMLKAGEQFTLTTEEIEGDE
                     SKVSITYEGLVQDVDAGRVILIDDGLIELKVVGKSEKEIFCEVINGGELGERKGVNVP
                     NVAVRLPAITEKDKDDIRFGVEQGIDFIAASFVRNAECVLEIKAYLKELGAPYVPIIA
                     KVENAEGIKNIDEIIRAADGVMVARGDLGVEIPAEEVPYLQKMIIQKCNMNFKTVITA
                     TQMLDSMMRNPRPTRAEVTDVANAVYDGTDAVMLSGETAQGKYPLEALQMMVHIIQNT
                     EQHLDYEGMLEKTGGHLKSGVSSAIGYSSVLAASNLNAKCIITPSVSGATARVVSNLR
                     PRQVILGVTPNERTLRRMSIYWGVKPIKSQAFNTTDDICDGAIELAKVKQFVETGEIV
                     VITAGIPSPNVKKERSATSNMMRIATVE"
     gene            complement(2570..3415)
                     /locus_tag="ACZPD4_07350"
     CDS             complement(2570..3415)
                     /locus_tag="ACZPD4_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378905.1"
                     /translation="MEKKVGRYAISNLTLYLLIAYGIGYMTRFLMPELLQWLTLEPGL
                     ILKGQIWRIVSWIFVAPAEKPIYFLLMVWLYYSLGTTLERTWGTFRYNVYIISGMLFT
                     VIGAFVLYGVLCATYDAAFIGGQTLLLTGSGLFSVSYIYMSIFLAFAVTYPDMEVLLY
                     FIIPIKMKWMGLVYGVMILYGIISGSMVTRIAIIASILNFIIFFLSTRNLSRYSPKER
                     ARKAKFKQQSAPHMTYANGARHRCAVCGRTELDDPCLEFRFCSKCNGNYEYCQDHLFT
                     HEHVK"
BASE COUNT          968 a          891 c          537 g         1144 t
ORIGIN      
        1 ttatgatcac acggttcatc ctcttttcca agtacggtaa tatggtgata cgctccatac
       61 attgccggac gcataagatt taccgcacat gcatccacac cgatatagtt tttataagta
      121 tttttctcgt ggatcgcttt tgtcacaaga cagccatacg gtcccatcat atagcgtcct
      181 aactctgtat aaatagcaac atctcccatt cctgccggaa caagaacttc ttcatacact
      241 ttccgaactc cctctccgat cgcatatata tcgttcggtt cttgatccgg tttatacgga
      301 atcccgattc cacctgagag attaataaac ttaatatgaa ctcccgcctc ttttgaaagt
      361 tttacagctt gttcaaataa aacttttgca agcatcggat aataatcatt cgtaacagta
      421 ttgcttgcca gaaatgcatg aagaccaaac tctttcgcgc ctttactttt taatatttta
      481 tatgcttcca aaatctgttc cggtgtcata ccgtattttg catctcccgg attatccata
      541 atatcgttac tgattttaaa taatcctccg ggattaaacc ggcagcttat tgtctccgga
      601 acatgaccga ttgcctgctc caaaaaatca atatgcgtaa tatcgtcaag atttatgata
      661 gccccaagct tatctgcata aataaattct tccgccggag tatcgttcga agaaaacata
      721 atgtcttctc ccgtaatttc aagagcttcc gacatcataa gttctgtata ggaagagcag
      781 tcgcagccgc agccatactc tttcaaaata tcgatcaaaa acgggtttgg cgtagctttt
      841 accgcaaagt attctttgta acccttgttc catgaaaacg cctctttaag cgcttttaca
      901 ttttctctga ttcctttttc atcataaaga tgaaacggag tcggatatat ttttgttatt
      961 ttctcgacct tctcttttgt cacaaacggt cttttatcca tcttctctca ctcctcatat
     1021 ttttatcttc agcctgattc ctattttttg cttttacata ttattccaca gttgcaattc
     1081 tcatcatatt acttgtggcg cttctctctt tttttacatt cggcgaagga attcccgctg
     1141 tgattacgac aatctctcct gtttctacaa actgcttcac ttttgccagc tcaatcgcgc
     1201 cgtcgcaaat gtcatccgtc gtattaaacg cctgtgattt aatcggtttt actccccagt
     1261 aaatcgacat tctgcgcaat gttctctcat tcggagtcac acctaaaatc acctgacgcg
     1321 gacgcagatt ggatacaact cttgccgttg ctcccgatac agaaggtgta ataatgcatt
     1381 tggcattcag atttgatgct gccagcacag atgaatatcc gattgcgctt gatacaccgc
     1441 tttttaaatg tcctcctgtt ttttccaaca ttccttcata atcgagatgc tgttctgtat
     1501 tctgaataat atgaaccatc atctgaagcg cttccagagg atatttcccc tgcgccgtct
     1561 ctcctgacag cattaccgca tctgttccgt catatacagc attggcaaca tctgtaactt
     1621 ccgctcttgt cggacgcgga ttacgcatca tagagtccag catctgggtt gccgtaataa
     1681 ctgttttaaa gttcatatta catttctgaa taatcatttt ctgaagatac ggaacttctt
     1741 ctgccggaat ttctactcca agatctcctc tcgcaaccat aaccccgtct gcagcacgga
     1801 taatttcatc aatattttta attccttctg cattctctac ttttgcaata atcggaacat
     1861 atggcgcacc gagttcttta aggtatgctt ttatttcaag tacacattct gcatttctca
     1921 caaaagatgc agcgataaaa tcaattccct gctctactcc aaaacggata tcatccttat
     1981 ctttttctgt gattgccgga agtctaaccg ctacattcgg cacatttact ccctttctct
     2041 caccgagttc tcctccgttt atgacttcgc agaaaatttc cttttcactt tttccaacaa
     2101 cttttaattc aatcaatccg tcatcaataa gaatcactct accggcatca acatcctgca
     2161 caagaccttc gtaagtgatc gaaacttttg attcatcccc ttcgatctcc tctgttgtca
     2221 gagtaaactg ctctccggcc ttgagcatta ttctttttcc gcctttcaat actcccgtac
     2281 ggatctccgg tccctttgta tcaagaagaa ttgcagtgtt tgtattcaat tcctgacgta
     2341 gttctttcag cagatccatt ctgctttttt gttcttcatg atctccgtgc gaaaaattga
     2401 agcgggcaac atccattccg ttttcaatca actttttcag cagttccctg tcattcgtgt
     2461 tcggtcccat tgtacaaact acttttgtct ttttcatcaa tcaattctct cctttataat
     2521 ctatctgata taagttttct caacacccgc ataagcaatt tacaaactgc tattttacat
     2581 gctcatgggt aaataaatga tcctggcaat attcataatt accgttacat tttgagcaaa
     2641 accgaaattc aagacacgga tcatccagtt ccgtccgtcc gcataccgca cagcgatgtc
     2701 ttgctccgtt ggcatatgtc atatgaggcg ccgactgctg tttgaacttt gccttgcggg
     2761 cccgttcttt cggtgaatac ctgctcaaat tgcgcgttga taaaaagaat ataataaagt
     2821 ttaaaatcga tgcaatgatc gcgattctcg ttaccatgct gcctgaaata attccataca
     2881 agatcatcac cccgtataca agccccatcc atttcatttt gatcggaata ataaaataca
     2941 ataacacttc catatccgga taagttaccg cgaatgccaa aaagatcgac atatagatat
     3001 aagaaacact aaataatccg cttcctgtta agagcagcgt ctgaccgcct ataaaagcag
     3061 catcatatgt cgcgcacaac accccgtaca gcacaaatgc cccgatcaca gtaaacaaca
     3121 ttcccgaaat aatatataca ttataccgaa atgttcccca cgtacgttcg agagtcgttc
     3181 ccaaagaata atacagccag accataagca gaaaataaat cggtttctca gccggagcta
     3241 caaaaatcca cgaaacaatt ctccaaatct gccctttcag tattaatccc ggttccagtg
     3301 tcagccactg aagaagttcc ggcataagaa atctggtcat ataaccgatt ccatatgcaa
     3361 taagcaagta caaagtcaaa ttacttatgg cataacgccc aacctttttt tccattttgt
     3421 caaaccagct caacaatcat tacctcacct tttaacattt ttatctaatt gtaactttca
     3481 catatctgtt tgtcaaacat atgttgtttc ttttcacacg tttaacacaa tctttatgta
//
LOCUS       DBMDXF010000189         3473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6163_length_3473_cov_1.358541, whole genome shotgun sequence.
ACCESSION   DBMDXF010000189 DBMDXF010000000
VERSION     DBMDXF010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6163_length_3473_cov_1.358541"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            201..1217
                     /locus_tag="ACZPD4_07355"
     CDS             201..1217
                     /locus_tag="ACZPD4_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1378906.1"
                     /translation="MENLRVIDVSEHQGTINWDAVKGHIDGAILRCGYGDNIASQDDK
                     QWKRNADECTRLGIPFGVYIYSYATSDAQAKSEAEHVLRMVSGYKLSYPIYLDLEQAG
                     TENGAIQRANIFGDIIEKAGYWCGVYANTNWWTNYLVGLERFVKWVAQYNSVCTYQGT
                     YDIWQYTSGGSVPGISGNVDMNHCYRDYPAEITGGDTKPTPPAVAPSGSTLDLVVGVM
                     QGKYGDGDARKNALGNRYNEVQNMINYIASASVDTLVKEVYAGKYGNGDTRKAALGNR
                     YNEVQNKINGSSGGGAVYYTVQSGDTLSGIAAKYGTNYQAIANLNGIQNPNLIYPGQK
                     LRVK"
     gene            1231..1665
                     /locus_tag="ACZPD4_07360"
     CDS             1231..1665
                     /locus_tag="ACZPD4_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378907.1"
                     /translation="MRLLEKNKQNLKYALQVGEVPIYERDEDGNIIYIEVDGQKVPVE
                     TGETEVGYSNPIDFRGNIAMSGGEAEAKSFGVDISEYDAVLLMEKNRIPIDETSLIWH
                     TSKVRYIDEQNTIVDRKSADYSIKRVQPSLNFTRYLLKRIVK"
     gene            1667..2200
                     /locus_tag="ACZPD4_07365"
     CDS             1667..2200
                     /locus_tag="ACZPD4_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016754.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HIR1378908.1"
                     /translation="MAKKVLKANIFSTSSIQELQKQLREYQDSLNKKCEIFTEELAKR
                     GVEIAKARVTTLDAIFTGDLLNNIHEKKGNGDKNSVIFFITADSRHAAFVEFGTGQLG
                     LEGSYPYPFPEGVEWNYNTGKTIFEIAPGKYGWFYPKDGKWYFTQGMPSRPFMYETSL
                     ELMQEIPQIAKKVFGGR"
     gene            2202..2597
                     /locus_tag="ACZPD4_07370"
     CDS             2202..2597
                     /locus_tag="ACZPD4_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378909.1"
                     /translation="MLDMLESQVITRIKTQFSKKLKDRYPNLKFTNSDRADTVPKFPT
                     VYIHEMTGAETGEDLQGDTINAVWSSFQIEVTTNTKMNDAKEVMNEVVRIMKTMRFQV
                     IATPEFQNTDSTYRRVARFRRMIADGDIL"
     gene            2658..3137
                     /locus_tag="ACZPD4_07375"
     CDS             2658..3137
                     /locus_tag="ACZPD4_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378910.1"
                     /translation="MIAGISTLGITFGYGVETTAGQKPAKFTKLNRINSIGGITIENE
                     QIDASAVEDAVKRYIRGAADTGGSFPVGVNFTSETKEEWKKVIEEYAKLTGEKRMWFQ
                     TIIPGFDESFFVIAQPPTALPQPEIGQNELLVMENNLTIEEFKGMDTKVDFEVVGGA"
     gene            3270..>3473
                     /locus_tag="ACZPD4_07380"
     CDS             3270..>3473
                     /locus_tag="ACZPD4_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378911.1"
                     /translation="MKTIQIGNEQYTLEFSFEAAENKAVVQRMFNALSMSYIGKRLDL
                     EGKNSKVEIAAAMIDGTADLISDM"
BASE COUNT         1207 a          540 c          852 g          874 t
ORIGIN      
        1 atatacagca aacgattctg tggtacatga ttctagcagc aattctagcc ggcctattag
       61 tagcttttgt ggcaatgtac ggttgggaaa aagtagcaga attgtggaaa cgattttaca
      121 gagtgaataa gaatgattta gaggatgagt aatcgtcctc ttttttgtac aggtgcaaat
      181 gccggagaaa ggagaaaatt atggaaaatt taagagtaat tgatgtaagc gaacatcaag
      241 gaactattaa ctgggatgcg gtaaaaggac atatagatgg ggcaatctta cgatgcggat
      301 atggagataa tattgcaagt caggacgata agcaatggaa aagaaatgca gatgaatgta
      361 caagacttgg gattccgttc ggagtttata tctattccta cgcgacaagt gacgcacagg
      421 cgaaaagcga agcggaacac gtccttagaa tggtaagcgg atataaactt tcgtacccaa
      481 tttatttgga tttggaacag gcaggaacgg aaaatggagc aattcaaaga gcgaatatct
      541 tcggggatat catcgaaaaa gctggatact ggtgcggagt ttatgcgaat acaaactggt
      601 ggacaaacta cctggtaggg ttggaacggt ttgtaaaatg ggtagcacag tataattcgg
      661 tttgcacata tcaaggaaca tacgatatct ggcagtatac gtcaggcgga tctgttccgg
      721 gaatttccgg aaacgtggat atgaatcatt gttatagaga ttatccagca gaaattacag
      781 gaggggatac aaaaccgacg ccgccggcag tagcaccatc tggatctacg cttgatcttg
      841 ttgttggagt tatgcaggga aaatatggag acggagacgc tagaaaaaat gctctaggaa
      901 atcggtataa tgaggtgcag aatatgatta actatattgc atctgcctcc gtagatacac
      961 ttgtgaaaga agtttatgcg ggaaaatatg gaaatggaga tacaagaaag gcggcgcttg
     1021 gaaacaggta caatgaggta caaaacaaga ttaatggttc ctccggcggc ggtgcagtat
     1081 actacacagt ccagtctggg gatacgcttt ctggaatcgc tgctaaatac ggtactaatt
     1141 atcaggcaat cgcaaatctg aacggtattc agaatccgaa cttaatttat ccggggcaga
     1201 agctacgcgt aaaataagga aggtgtctgt atgcgacttt tagaaaaaaa caagcagaat
     1261 ttaaagtatg cgttacaagt cggggaagtt ccgatttacg aacgagacga agacggaaac
     1321 atcatatata ttgaggtgga cggtcagaaa gttccggtag aaacaggaga gacagaagtc
     1381 gggtattcaa atccgattga ttttagagga aatattgcaa tgtccggcgg tgaagcagaa
     1441 gcaaagtctt ttggagtaga tatcagtgag tatgacgcgg ttcttctcat ggagaaaaac
     1501 agaataccta ttgacgaaac gtctctaatt tggcatacaa gcaaagttag gtacatagac
     1561 gaacaaaata ccattgtaga cagaaagtcg gcggattact cgattaaacg tgttcagcca
     1621 agtcttaatt ttacaaggta tcttttgaaa aggattgtga agtagcatgg caaagaaagt
     1681 gttaaaagcg aatatttttt caacttccag tattcaagag ttgcagaagc agttaagaga
     1741 atatcaagac tcacttaaca agaaatgtga aatattcaca gaagaattag caaaacgagg
     1801 tgtagagatt gcaaaagcaa gggttactac acttgacgcg atatttacag gagatcttct
     1861 gaataacata catgaaaaaa agggtaacgg agataaaaat tccgttatct tttttattac
     1921 ggctgattcg agacatgccg catttgttga gttcggaact ggacagcttg gacttgaagg
     1981 tagctaccca tatccattcc cggagggcgt ggagtggaat tataacaccg gaaagacaat
     2041 ttttgagatt gcgcccggaa aatacggatg gttctatccg aaagatggaa aatggtattt
     2101 tacgcaaggt atgccgtcaa gaccgtttat gtatgaaaca tcattggaac tcatgcaaga
     2161 gattccgcag attgcaaaaa aggtatttgg agggcggtaa tatgctagat atgttggaat
     2221 cacaagttat cactcggata aagacgcagt tttctaaaaa actgaaagac cgttatccaa
     2281 atttaaaatt tacaaactct gacagagccg atactgttcc gaaattccca accgtgtata
     2341 tacacgaaat gacgggagcg gaaacaggag aagacttaca aggagatacg atcaatgctg
     2401 tttggtcttc ttttcagatc gaggtaacaa caaataccaa gatgaatgat gcgaaagaag
     2461 tgatgaatga ggttgtacga attatgaaaa caatgaggtt tcaagtaatt gcaacaccgg
     2521 aatttcagaa cacagacagt acatatcgaa gagtagcacg ttttcggcga atgatagccg
     2581 atggcgatat tttataagac cgatcataaa aagtgatcgc ttactgcaaa aaattagcgg
     2641 tggaaaggag aaaaaatatg attgcaggaa tttctacatt aggaattaca tttggttatg
     2701 gagttgaaac aactgccgga caaaaaccag ctaaatttac aaagcttaat cgaattaatt
     2761 caattggtgg aatcacgatt gaaaatgagc agattgatgc gtctgcggtt gaagatgctg
     2821 ttaaacgata tatccgaggg gcagcagata cgggaggttc attccctgtc ggtgtaaact
     2881 tcacaagtga aacaaaagaa gaatggaaaa aggttatcga agaatacgca aagcttacag
     2941 gtgaaaaaag aatgtggttt cagacgatta ttccgggatt tgatgaatca ttctttgtaa
     3001 tcgcacagcc accaacagca ctcccacagc cggagattgg tcagaacgaa ttgctcgtaa
     3061 tggaaaataa ccttactatt gaagagttta agggaatgga cacaaaagtt gattttgaag
     3121 ttgtgggggg agcttaagct acttagatac aaaagccggt ctaagtagcg tttctgatga
     3181 aatggcttat acagaacttg aagaaacata ttaaaatatg agcggggcag tcttcggact
     3241 gccccctctg attaatcgga gggaaaaata tgaaaacaat tcagattggg aatgaacagt
     3301 atacgttaga atttagtttt gaagcggcag aaaataaagc agttgtgcaa aggatgttta
     3361 atgctttgtc gatgtcttat attggaaaaa gattggattt agaaggtaaa aatagcaaag
     3421 tagaaattgc tgctgcaatg attgacggaa cagcagattt gatatctgat atg
//
LOCUS       DBMDXF010000190         3462 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6192_length_3462_cov_1.814936, whole genome shotgun sequence.
ACCESSION   DBMDXF010000190 DBMDXF010000000
VERSION     DBMDXF010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_3462_cov_1.814936"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="ACZPD4_07385"
     CDS             <1..283
                     /locus_tag="ACZPD4_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR1378912.1"
                     /translation="DHKYDVTGSLCENNDKFAVDRMLPLIERGDYLVIHDTGAHGFAM
                     GYNYNGKLKSAELLLKENGEVKLIRRAETPADYFSTFDCFEIGKKLMNV"
     gene            693..775
                     /locus_tag="ACZPD4_07390"
     tRNA            693..775
                     /locus_tag="ACZPD4_07390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Leu,seq:caa)
     gene            1037..2899
                     /locus_tag="ACZPD4_07395"
     CDS             1037..2899
                     /locus_tag="ACZPD4_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR1378913.1"
                     /translation="MIKKKKLLICALLMSMVVSFMPGMESNAAELSGEDQVTATLQDE
                     NSQRLEETTAMDEDGAIYEIHEEKGIVAEGTPSQNAMFLAKTSGQSKVVNFNTKGSAV
                     TSFTYGGGSGYTNGAYGADAAYLGMTDDGKVRFMLSGVTGTVNASEVQVVEYAAVSDH
                     VSYYAVSGGKLIHYISQDLNKLPVSFINNGTAPSYLNEGVKYYSYDGHYFYTDYAVML
                     SDYQNNTNGQNAVNAGNAFYNFFQFKNMREATKYSGEELNVMLQSAMSAAGVDTASSK
                     LSGTGLSFVKYQNVYSVNALLSMGIAINESGWGTSWICRNKNNIFGLNAVDSAPGISA
                     DTYASIDDCIRSFMKEWMDEGYLDSSDWRNHGTYLGDKSSGINVSYASDPYWGEKAAA
                     HAWNLDFIGGNKDCQIQEETPNVPNEPETDVPETPDVPSEPETNVPETPDVPSEPEIN
                     APETPDVPSEPETPDVPSEPETNAPETPNVPGEPETNAPETPNVPGEPETDVPETPDV
                     PDVPSQPETDASEIPDANRIETPGTGNVQNSGNAGTETTPGGTSVSGSQTGTKEETTV
                     ETTVETTPTVNPVSATVPSPKTGDEGQLAVWIVLLGVSLTAGVVVIGKKKSLYR"
BASE COUNT         1167 a          550 c          840 g          905 t
ORIGIN      
        1 tgatcataaa tatgatgtga caggttcgct ttgtgagaat aacgataagt tcgctgttga
       61 caggatgctt cctctgattg agcggggaga ttatcttgtg attcatgata caggggcaca
      121 tggatttgca atgggttaca attataatgg taaattaaaa tcagccgaac ttcttcttaa
      181 agagaacgga gaagtaaaac ttatcagaag ggcagaaact cctgctgatt acttttcaac
      241 atttgattgt tttgaaattg gaaagaaact tatgaatgta taatttttta gaatatattt
      301 tattattaac ggaaatagtt ttaaataaaa gaattgttta gactttcttt ttaaatagtg
      361 gaaaggggaa attatgaaaa aattacttat tatgatgtgt gtattattta ttattaattt
      421 agtagctatc ataattcctt tatggagttc ggagaacaca atgattattt tatatcgcat
      481 ggctatattt tgtaaccttg cagtatttgt gccttcaata actttaatat atagagagaa
      541 agggaaaact caataagaaa attgtgatat tttatttccg tatggcatac tcttttaaaa
      601 tgtttaaaat aatttgtaaa acagtaaaaa aagcttgcaa tataaaaaaa caagtgatat
      661 aataattctt ggcttcaaaa agaagccatt atgcggatgt ggcggaatgg cagacgcaca
      721 agactcaaaa tcttgcgtag gtgactacgt gtgggttcaa gtcccaccat ccgcattaaa
      781 aaaggattct gtatgacggt gttatacaga atcctttttg tcatatggga aattttattt
      841 tcgtacgagg ttacttggaa tttgttttgt taaaagcttg aaaatcggac tgaatagtga
      901 taatactcat tgtatacttg tatacaatat taaaaagtaa tgcttgccaa tgatggaata
      961 aaatgttatg gtagttatgt atcagtgtag attagtataa ataattaata gcagggttaa
     1021 ggagggccat acacaagtga taaagaaaaa gaaactactg atatgcgcac ttttgatgtc
     1081 gatggtagta agttttatgc cgggcatgga aagtaatgct gcagagctat cgggggagga
     1141 tcaggtcaca gcgacacttc aggatgaaaa tagtcagcgt cttgaggaga caacggctat
     1201 ggatgaagat ggagcaattt atgaaattca tgaggaaaaa ggtattgttg cggaaggaac
     1261 gccatcacaa aatgcaatgt ttttggcaaa aacatccggg cagagtaaag tagtcaattt
     1321 taatacaaaa ggcagcgctg taacttcttt tacatatggc ggcggaagcg gatatacaaa
     1381 cggagcttat ggtgcagacg cggcatattt aggtatgact gatgatggga aagttcgatt
     1441 tatgctgtca ggagtgacag gaacggtaaa tgcttcagaa gttcaggttg tggagtatgc
     1501 ggcggtttcg gatcatgtaa gttattatgc ggtatccggt ggaaaactga tccattatat
     1561 ttcacaagat ttaaataaac ttccggtttc ttttataaat aacggaacag caccttctta
     1621 tttaaatgaa ggtgtaaagt attacagcta tgatggacat tatttttata ccgattatgc
     1681 agtgatgctt tcggattatc aaaataatac aaacggtcaa aacgcagtga atgcagggaa
     1741 tgcgttttat aactttttcc agtttaaaaa catgagagaa gctacaaaat acagtggcga
     1801 ggaattgaac gtaatgctcc aatcggcaat gagcgctgca ggagttgaca cagcttcttc
     1861 aaaattatcg ggaacaggac tttcatttgt aaaataccaa aatgtttata gtgttaatgc
     1921 gcttctctct atgggaatag caattaatga aagcggttgg gggacaagtt ggatctgccg
     1981 gaataaaaat aatatttttg gcttgaatgc ggtggacagc gctcccggaa taagcgccga
     2041 tacatatgcg agcattgatg attgtattcg cagttttatg aaagagtgga tggatgaagg
     2101 ttatttagac agctcggatt ggagaaacca tggaacttat cttggggata agagcagcgg
     2161 aattaatgta agttatgcat cggaccctta ctggggagaa aaggcggctg cccatgcatg
     2221 gaatctggat tttataggtg gaaataaaga ttgtcagatt caggaagaaa cgccaaatgt
     2281 accgaacgaa cctgagacag acgtacctga aacaccggat gtaccgagtg aacctgaaac
     2341 aaacgtaccg gaaacgccgg atgtaccgag tgaacctgaa ataaatgcac cggaaacgcc
     2401 ggatgtacca agcgaacctg aaacgccgga tgtaccaagc gaacctgaaa caaatgcacc
     2461 ggaaacaccg aatgtaccgg gtgaacctga aacaaatgca ccggaaacgc cgaatgtacc
     2521 gggtgaacct gaaacagatg taccggaaac gccggatgta cctgacgtgc cgagtcaacc
     2581 tgagacagat gcatcagaaa taccggatgc aaacagaatt gaaacgccgg gaacgggaaa
     2641 tgtgcaaaac agcgggaatg caggaacgga aacgacaccg ggcggaactt ctgtatcagg
     2701 aagtcagaca gggacaaaag aagaaacaac agtagaaaca acagtagaaa caactcctac
     2761 ggtaaatcca gtcagcgcaa cggttccatc tcctaaaaca ggggatgaag ggcagttggc
     2821 agtgtggatt gttcttcttg gagtatcgct gacagcgggt gtagttgtta tcggaaagaa
     2881 aaagtcctta tatagataaa tagataagta taaaataata aaagcgaaaa gtgaatgagt
     2941 gcacttttcg ctttttggtt tgcgcgccat gggcgcgctc taacgggtgt aagtcccgaa
     3001 cacgcccaga tagtgggaag tgtatagctg aacagcaagt ccatcgtgag gtggaatctg
     3061 aagaaagttg taagcaaatc tctggtccga cggacagaaa tcacatataa ggctaggctg
     3121 tgagagataa gttggccaaa agcaacgaag tctaataact atcacttttg tagcagcaga
     3181 gtagatgtgg cggatatatg gagagaaaga gcgtgaacct taagcaggag gtctcacaga
     3241 ggtctcatca gcctagtaac aacgaactgt gagaagtcag ccgagcccat agcagtgaag
     3301 aagtttttgt aatggaaacg gagcaaaggg gcgaacaatc aataagtttg agtatgtttc
     3361 gtattgcaga aatgacagca tctgtcgtaa ccaatcgggg aaagggtggt caaatcaagc
     3421 gaggcggaaa gggaagaaca ctgctccatc gtttgctcca ac
//
LOCUS       DBMDXF010000191         3442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6239_length_3442_cov_2.006286, whole genome shotgun sequence.
ACCESSION   DBMDXF010000191 DBMDXF010000000
VERSION     DBMDXF010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6239_length_3442_cov_2.006286"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            178..900
                     /locus_tag="ACZPD4_07400"
     CDS             178..900
                     /locus_tag="ACZPD4_07400"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HIR1378914.1"
                     /translation="MAKIIDTHSHILPGIDDGAGSWKEALCMLKTAQAQGIRRVIATP
                     HWGGPFGYTDPVQIRELCFTLQEKARRHNISVKIYQGQEVMYTEEAADRIANGEILTL
                     ADSRYVLAEFYQGIGYSGIFRAVQRFTQSGYSPIIAHAERYEALKDIGRLEELRKQGA
                     YIQLNFRAIGGKWHDFTTRWCRKALKEGFVHFVGTDMHNMRTRKPETESAVVWMEKHL
                     EPDYVKKLFLKNPEKILRNERL"
     gene            1088..1846
                     /locus_tag="ACZPD4_07405"
     CDS             1088..1846
                     /locus_tag="ACZPD4_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR1378915.1"
                     /translation="MEKTHDEEMAIDLWELISALKKRALIILAACLLGGVVAGVYTKL
                     CITPMYTATSSMLVTTQETTLTSIADLQLGSALTGDYSILATSRSVLEEVIEDLGLNM
                     NHHQLRGSITITNPEGTHIMEVTARSSDPETAKKIADTMAVVSAQYIKEKMEVAPPKI
                     IEEAEVPSSPVSPSMNKNVMMGALAGFALAAAIVILFAIMNDSIKTEDDIERALGIPT
                     LAVIPDRKDYIDDHGGSKKKKGKRRRQSKWRNKR"
     gene            1828..2550
                     /locus_tag="ACZPD4_07410"
     CDS             1828..2550
                     /locus_tag="ACZPD4_07410"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR1378916.1"
                     /translation="MAEQKVIMTDPRQEDYFYQEAIKTLRTNIQFAGMDVKTILFTSC
                     YPNEGKSDITFQLSQEIGKLGKKVLLLDADIRKSILASRYGVGKQVHGLSNYLSGQAS
                     IAEIIYHTNYENMDIIFSGSFAPNPSELLGQEAFGTLLGALRERYDYIIVDTPPIGSI
                     IDAAIVAKQCDGAVLVFESELVSSKEAVRIKEQLQMTGCRLIGAVLNKVDIKKNKYYH
                     KYNYYYHGYQEKKEVTKKNKEK"
     gene            2612..>3442
                     /locus_tag="ACZPD4_07415"
     CDS             2612..>3442
                     /locus_tag="ACZPD4_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1378917.1"
                     /translation="MKKKKYLKWWIAAASAVIIVGKGIFVYTQYKAAQTSGDVATKEG
                     TAASASDITWNGKTYSYNEHLSNFLFLGIDTKEKAETKTGQADAGQADALYLLSWNRL
                     EGDITVISIPRDTMTQIETFGPGGESLGKSRDHISLSYAYGDGGYESCELAENAVSEL
                     FYGLPIDGYCALNMDGLPVLTDSVGGVTVTVPNDSLAEVDARYAKGAVVTLKSEDTEQ
                     FVRYRNTEISQSAITRMERQQEYIRAFGEALKKASADKELVTDLYQAIEPYMVTNMGK
                     S"
BASE COUNT         1148 a          583 c          910 g          801 t
ORIGIN      
        1 cagcatatct atctaatgtg ttttattgaa agttgataaa tgacataggg atatgctgtt
       61 ttggcgtgtc atgaaaaaac ttaaacacat taatgcgtta tcgcaatgat agaatgaagc
      121 gccgtgaaaa tgttgaaact aggtattgga attcaaagtt aaggagcggc gcacaaaatg
      181 gcaaaaataa ttgatacaca cagtcatata ttacccggga tcgatgacgg ggctggttca
      241 tggaaagaag cgctttgcat gttgaaaaca gcgcaggctc agggaatcag aagagtgatc
      301 gcaactcctc actggggagg acctttcggt tataccgatc cggtgcagat cagagaactc
      361 tgttttacat tacaggaaaa agcaagacgt cacaatatca gtgtgaaaat ttatcagggg
      421 caggaagtga tgtacaccga ggaagcggcg gacaggattg caaacggcga gatccttact
      481 ttggcggaca gccggtatgt actggctgaa ttttatcagg gaatcgggta ttcgggaatt
      541 tttcgggcag tacagcgctt tacacagtcg ggatacagtc cgatcatcgc tcatgcagag
      601 agatatgaag cgctgaaaga tatcgggcgt ctggaagaac tacgaaagca gggagcatat
      661 atacagttga atttcagggc gatcggggga aagtggcatg attttacaac acgttggtgc
      721 aggaaagctc tgaaagaagg atttgttcat tttgtcggaa cggatatgca taatatgaga
      781 acgagaaaac cggagacaga atccgcagta gtgtggatgg agaagcatct ggagccggat
      841 tatgtaaaaa aactgttttt aaaaaatccc gagaagattt taagaaacga gcgtttgtaa
      901 gaaaaacatt gtaaaacata gtcaatttat gtaaaaaaga cgcattgtaa aatgagaaac
      961 agggaaaaag tgaagaaaat actggtaaat ggaattaatt tatttcctta tagaaaaata
     1021 tgatatataa ttagaaggat taatatatta cgaacagaga gacggataat agaggaagtg
     1081 ataaaatatg gaaaaaacac atgatgaaga aatggcaatc gacctgtggg agttaatttc
     1141 cgcactgaaa aagcgggcgc tgattatttt ggcggcgtgt ctgcttggcg gtgttgtggc
     1201 aggtgtatat acaaaacttt gcatcacccc gatgtatacg gcgacatcat cgatgcttgt
     1261 gacaacacag gaaacaacgc ttacgtctat tgcggacctt cagcttggaa gcgctcttac
     1321 gggagattac agtattcttg cgacaagccg gagcgttctt gaagaggtga tcgaagatct
     1381 gggtctgaac atgaatcatc accaactgag aggaagtatt acgatcacca atccggaagg
     1441 aacgcatatt atggaagtga cggccagaag ttccgatccc gagaccgcaa aaaagattgc
     1501 ggacactatg gcggttgttt cagcgcagta tataaaagaa aaaatggagg tggctcctcc
     1561 taagatcatc gaagaggcgg aagttccgtc atccccggtc agcccgagta tgaataaaaa
     1621 cgttatgatg ggcgcgctgg caggatttgc tcttgcggcg gcgatcgtga ttttgtttgc
     1681 gatcatgaat gattcgatca aaacagagga tgatatcgaa agagcgctcg gcattccgac
     1741 acttgccgtg attccggaca gaaaagatta tatagatgat catggcggca gtaaaaagaa
     1801 gaaaggcaaa agaaggaggc agagtaaatg gcggaacaaa aggtgataat gacagatccg
     1861 agacaagagg attattttta tcaggaagca attaagacgc tgcgaacgaa tatccagttt
     1921 gccggaatgg atgtaaagac aattctgttt acaagctgct atccgaatga aggaaagagc
     1981 gatattacgt tccagctttc gcaggaaatc ggaaaacttg ggaaaaaggt acttcttctt
     2041 gatgccgata tccgcaaatc tattcttgca tcacgatacg gagtaggaaa gcaggttcac
     2101 ggattatcaa attatttgag tggtcaggct tctatcgcag agattatcta tcatacgaat
     2161 tatgaaaata tggatatcat tttttcgggt tcgttcgcgc cgaatccatc agaactgctt
     2221 ggacaggaag cgtttggaac actgttggga gcgttaagag agcgttatga ctacatcatt
     2281 gtagatacgc cgccgatcgg aagtattatt gatgcggcga tcgttgcaaa acagtgtgac
     2341 ggagcggtac ttgtatttga aagtgaactt gtgagcagta aagaggcggt tcggatcaaa
     2401 gaacagcttc agatgacagg gtgcagattg atcggcgccg tgctcaataa ggttgatata
     2461 aagaaaaata aatattatca caaatataac tattactatc acggatatca ggaaaaaaag
     2521 gaagttacaa agaaaaataa agagaaataa aacggtattc ttaagtaata tttgaacgat
     2581 cagataatgg gaggggatca gaaagaaaaa aatgaaaaag aaaaaatatt tgaaatggtg
     2641 gatcgcggcg gcatctgcgg ttatcattgt aggaaaaggg atatttgtat atacgcagta
     2701 taaggcggcg cagacgtcag gagacgttgc tacaaaagag ggaacagccg catcggcatc
     2761 ggatatcaca tggaacggaa agacatatag ttacaatgag catttgagca actttttgtt
     2821 cctcggtatt gatacaaaag aaaaggcaga aacaaaaaca ggacaggcag acgccggaca
     2881 ggcggacgca ttatatctgc tttcatggaa cagactggaa ggagatatta cagtgatcag
     2941 tattccgaga gatacgatga cccagatcga gacattcggt ccgggcggag aaagtctcgg
     3001 aaagagcaga gatcatatca gtttatctta cgcatatggc gacggagggt atgaaagctg
     3061 cgagctggca gaaaatgcag tgtcagaatt gttttacggt ttgccgatcg acgggtactg
     3121 cgctctgaat atggatggac tgccggttct gacagacagc gtcggcggtg tgacagtaac
     3181 cgtaccgaat gacagcttgg cggaagttga cgccagatat gcaaagggcg cagtggtaac
     3241 actgaaaagc gaagacacag aacagttcgt tcgatacaga aatacagaaa tcagccagag
     3301 tgcgatcacg cgtatggagc gtcagcagga atatatccga gcattcggtg aagcgttaaa
     3361 aaaggcatct gccgataaag aactcgttac ggatctgtat caggcgattg aaccgtatat
     3421 ggtaacgaat atgggaaaaa gt
//
LOCUS       DBMDXF010000192         3429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6266_length_3429_cov_1.392428, whole genome shotgun sequence.
ACCESSION   DBMDXF010000192 DBMDXF010000000
VERSION     DBMDXF010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6266_length_3429_cov_1.392428"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /gene="polA"
                     /locus_tag="ACZPD4_07420"
     CDS             <1..2004
                     /gene="polA"
                     /locus_tag="ACZPD4_07420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR1378918.1"
                     /translation="KIIMEYQTIENAYAHASELKPPRASKNIQEFWEQAKMSKVLATI
                     ETHADFEFDIEKAHYENPYTEEAYKIFERLRFKNMLGRFSIETKENKIEKIFREVTEK
                     EEIERIFAMAEKAQCVGVALSKDEGNVLPLFAHPSGFGRIAIAWSEKDVVTIPCDLST
                     DMEFLFAKLSHVAEKVSCFSVCGLKEILPYIKNVKQSSAFDVIVAAYLLNPLKSDYTY
                     EDVAEQYLGIAGGIQAELNVKCCYEAYTAFAAASVLDNKLKEAEMDRLFYEIEMPLVF
                     TLYEMESAGVKVEAEALKLYGDQLGEQIIQLEHQIYEMAGEEFNINSPKQLGVILFEK
                     LNMPHAKKTKTGYSTAADVLEKLAEEYPIVSKILEYRQLAKLKSTYADGLAVFIGDDG
                     RIHGKFNQTVTATGRLSSTEPNLQNIPVRMELGRMIRKVFVPKEDCVFVDADYSQIEL
                     RVLAHCSGDEQLIKAYREATDIHRMTASQVFHTPFDEVTDLQRRNAKAVNFGIVYGIS
                     SFGLSMDLSITRKEAAEYIEHYFETYPGVKKFLDDSVENAKEKGYAVTLFGRRRPVPE
                     LKSSNFMQRNFGERVAMNAPIQGTAADIMKIAMIDVHKELKKRNMKSRMILQVHDELL
                     IEAECSEVDEVKEILRDKMENAVKLSVPLIVDMHTGKNWYEAK"
     gene            2044..2628
                     /gene="coaE"
                     /locus_tag="ACZPD4_07425"
     CDS             2044..2628
                     /gene="coaE"
                     /locus_tag="ACZPD4_07425"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846390.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1378919.1"
                     /translation="MKVLGITGGVGSGKSEVLDYLESRYGAYVCQMDEVAKRLEKRGE
                     ICFRKIVDRFGEAVVGTDGELDRKKLGEIVFSDEGKRKILNEIVHPPVLDYVKKDIEK
                     RKKEGRKLYVLESALLAEAGQELCDKIWYIYTEENVRRIRLERSRGYTQEKITQMMAS
                     QLSVEQFRENCTDVIDNSGDFKETKRQIGAKIQL"
     gene            2625..3422
                     /locus_tag="ACZPD4_07430"
     CDS             2625..3422
                     /locus_tag="ACZPD4_07430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1378920.1"
                     /translation="MRMCSIASGSSGNCIYVGSDDTHLLVDTGISKKRIEEGLKTLEL
                     KGEDLDGILITHEHSDHIQGLGVFSRKYEIPIYATPGTIMGIKSYSGLGKMPEGLYRE
                     IRADEPFELGDMTVKPFAISHDANEPSGYRLEQGGKSVAVATDLGKYDSYTVECLRNL
                     DAVLLEANHDIHMLEVGGYPYYLKQRILGDRGHLSNELSGRLLCDILHDNLKHIVLGH
                     LSKENNYAKLAYETVKLEVTLADNEYKGDDLDMFVANRDTVSDIITV"
BASE COUNT         1141 a          497 c          904 g          887 t
ORIGIN      
        1 aaaattatta tggagtatca aacgattgaa aatgcgtatg cacacgcgtc ggaattaaag
       61 ccgccccggg caagtaagaa tattcaggag ttttgggaac aggcaaaaat gagtaaagtg
      121 cttgcgacga ttgaaacaca tgcggatttt gagtttgata ttgaaaaagc gcattatgaa
      181 aatccttata cggaagaagc gtacaaaatt tttgagcggc ttcgttttaa aaatatgctt
      241 ggacgttttt ctatagaaac gaaagaaaat aaaatagaaa agatatttcg agaagttacg
      301 gaaaaagaag aaatagaacg tatttttgcg atggcggaga aagcacaatg tgtcggggta
      361 gcattgtcaa aagatgaagg aaacgtactc cctctttttg cacatccttc gggattcggg
      421 agaattgcca tagcttggag tgagaaagat gttgttacga ttccatgtga tctttctacg
      481 gatatggaat ttttatttgc aaagttgtcg catgttgcgg aaaaagtgag ctgtttctca
      541 gtatgtggtt tgaaagaaat tcttccgtat atcaaaaatg taaagcaaag cagcgcgttt
      601 gatgtgatcg tggcagctta tctgttaaat cctctaaaaa gcgattatac gtatgaagat
      661 gtggcagaac agtatcttgg gattgcaggc gggatacagg ctgaacttaa tgtgaagtgt
      721 tgttatgagg cctacacggc gtttgcggcg gcatcggttc ttgataataa attaaaagaa
      781 gctgaaatgg atcgtctttt ttatgagatt gaaatgcctt tggtttttac attgtacgag
      841 atggaatcgg caggtgtgaa agtagaggct gaagctttga aattgtacgg agatcagttg
      901 ggagagcaga tcatacaact tgagcatcag atttatgaga tggcggggga agagtttaat
      961 attaattccc cgaaacagct tggagttatt ttatttgaaa aactaaatat gccacatgcg
     1021 aaaaagacaa aaaccggata ctctacagcg gcagatgtgt tggaaaaact ggcagaagaa
     1081 tatccgatcg taagcaaaat attagaatat cgtcagctgg caaaactgaa atcaacttat
     1141 gcggacggtc tggcggtttt tatcggcgat gacggcagaa tacacggaaa gttcaatcaa
     1201 acggtaactg cgacaggaag attgagcagt acagaaccga atttacaaaa tattccggtg
     1261 cggatggaac ttggtcggat gatcaggaaa gtgtttgtgc caaaagaaga ctgtgttttt
     1321 gtcgatgccg attattcgca gatagaactt cgtgttttgg ctcattgttc gggcgacgag
     1381 cagttgatca aagcatatcg ggaagcgact gatattcatc ggatgacggc gtcgcaagta
     1441 tttcacactc cgtttgatga agtgacagat ttgcaaagaa gaaatgcaaa agccgttaat
     1501 ttcggaattg tgtacggtat cagttccttt ggattaagta tggatttgag tattacaaga
     1561 aaagaagcgg cagaatacat tgagcattat ttcgagactt atccgggagt gaaaaagttt
     1621 cttgacgatt cggtagaaaa tgcaaaagaa aaaggatatg cagttacgtt gttcggcaga
     1681 aggagacctg tgccggaatt aaaatcgagc aattttatgc aaaggaattt cggagagaga
     1741 gttgcgatga atgctccgat acaaggaacc gcagcagata tcatgaaaat tgcgatgatc
     1801 gatgtccata aagaacttaa gaaaagaaat atgaaatcgc gtatgatttt gcaggtccat
     1861 gatgaattgc ttattgaggc agagtgttcg gaagtagatg aagtgaaaga aattttaaga
     1921 gataagatgg aaaacgccgt gaaattgtct gttccgctaa ttgttgatat gcataccggg
     1981 aaaaattggt atgaggcaaa atagacgatt tgtcgggaag cagtataaag gatagaaaat
     2041 agaatgaaag ttttaggaat caccggcgga gttgggtcgg gaaagagcga agtattggat
     2101 tatttggaaa gcagatatgg agcttatgtt tgtcagatgg atgaagtggc aaagcgattg
     2161 gaaaaaagag gagaaatatg tttccggaaa atcgtggaca ggttcggaga agcggtcgtc
     2221 ggaactgacg gggaattaga tcgtaaaaaa cttggcgaaa ttgtgttttc cgatgaagga
     2281 aaacggaaga tcctgaatga aattgttcat ccgccggtgt tggattatgt gaagaaagat
     2341 atagaaaagc gaaagaagga agggcggaag ctgtacgtgt tggaatcggc tcttctggca
     2401 gaagcagggc aggaattgtg cgataaaatc tggtatattt atacagaaga aaatgttcgg
     2461 agaatccgcc tggaacgatc aagagggtat actcaggaaa agatcacaca aatgatggca
     2521 tctcagcttt cggtggagca atttcgtgaa aattgtacgg atgtaattga taacagcgga
     2581 gattttaaag agacaaaaag acaaatagga gcgaagatac aattatgaga atgtgcagca
     2641 ttgcaagtgg gagcagcggc aactgtatct atgtcggaag cgatgatacg catttattgg
     2701 tagatacggg aatcagcaag aaaagaatag aagaagggtt gaaaacatta gaattaaaag
     2761 gcgaagattt agatgggatc ttaattactc atgaacactc ggatcatatt caaggattag
     2821 gagtattcag cagaaaatat gagattccga tctatgctac gccgggaacg atcatgggga
     2881 tcaaaagtta ttccggtttg gggaaaatgc cggaaggact atatcgcgag atccgtgcgg
     2941 atgaaccttt tgaactggga gatatgactg taaaaccgtt tgcgatttca catgatgcaa
     3001 atgaaccttc gggatatcga ttggagcagg gaggaaaatc agttgcggtt gcaacggatc
     3061 ttggaaaata cgacagctat acagtcgaat gtctgagaaa tcttgatgca gttctccttg
     3121 aggcaaacca tgacattcat atgttggaag tcggaggata tccgtactac ttgaaacaga
     3181 ggattctcgg tgacagggga catttatcaa atgaattgtc aggtcgtctg ctttgtgata
     3241 tactacatga taatctgaaa catatcgtgc tcgggcattt gagcaaggaa aataattacg
     3301 caaaacttgc atatgagacg gtcaaattag aagttacgct ggcagataac gagtacaaag
     3361 gcgacgatct ggatatgttc gtagcgaaca gagataccgt ttcggatatt attacggttt
     3421 gatcaagga
//
LOCUS       DBMDXF010000193         3425 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6272_length_3425_cov_2.041817, whole genome shotgun sequence.
ACCESSION   DBMDXF010000193 DBMDXF010000000
VERSION     DBMDXF010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6272_length_3425_cov_2.041817"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="ACZPD4_07435"
     CDS             complement(<1..637)
                     /locus_tag="ACZPD4_07435"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR1378921.1"
                     /translation="MQQFDIKKPLPHKPLFNPDGDTDVLKRRMIGGNTTNLNDYNNLK
                     YTWASEWYRQAMNNFWIPEEINLSQDIKDYPNLSPAERKAYDKILSFLVFLDSIQTAN
                     LPCVGEYITANEVNLCLNIQTFQECVHSQSYSYMLDTICSPDERMDILYQWKTDEHLL
                     KRNTFIGECYNEFQVKKDARTLVKVMMANFVLEGIYFFSGFMFFYNLSRNGK"
     gene            complement(688..2946)
                     /locus_tag="ACZPD4_07440"
     CDS             complement(688..2946)
                     /locus_tag="ACZPD4_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR1378922.1"
                     /translation="MTLITDSFVCRTLQFQERLEKLLYDIHTEFPEKVYSLELLFEKF
                     SGFLKTDMDDGERFDALIKAAAELTSQEAPRWEMIAGWLLDFRFSEKLKEEEKSIGIS
                     DLYEKIVYLTEKGLYGSYIMENYSKEEIVEAESYICKERNRLFNYSGLDLLIKRYVIS
                     THRHVPLESPQEMFLGIALHLAMNEKRKIRLMWVREFYDMLSKLEVTMATPTLANARK
                     PFHQLSSCFIDTVPDSLKGIFRSIDNFAMVSKYGGGMGLYFGKVRASGSMIRGFEGAA
                     GGVIRWVRIVNDTAVAVDQLGVRAGAVAVYLDAWHRDLPEFLQLRTNNGDERMKAHDV
                     FPAVCYPDLFWKMAGENLDQDWYLMCPHEIMTVKGYCLEDSFGEEWERRYWDCVHDAR
                     IKKRTVLLKDLIRLIIKSAAETGTPFAFNRDTVNRANPNPHKGMIYCSNLCTEIAQNM
                     SAISTVETKIQTEDKDTVVVNTTKAGEFVVCNLASLSLGNLPVNDPAYVRKVVRTVVR
                     ALDNVIDLNFYPVPYAKITNHTYRSIGLGVSGYHHMLAKNKIKWQSEEHLAFVDKLFE
                     QINYAAIEASSDYAKEKGSYRYFEGSDWESGAYFEKRGYCSDEWKELREKVHRQGMRN
                     AYLLAIAPTSSTSIIAGTTAGIDPVMNKYFLEEKKGSMLPRVAPDLSPETYWYYINAH
                     HIDQNWSVRACGVRQRHVDQAQSMNFYITNDYTMRQVLNLYLKAWECGVKTVYYVRSK
                     SLEVEECESCSS"
     gene            complement(2973..3374)
                     /locus_tag="ACZPD4_07445"
                     /pseudo
     CDS             complement(2973..3374)
                     /locus_tag="ACZPD4_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
BASE COUNT          938 a          877 c          578 g         1032 t
ORIGIN      
        1 tttttccgtt cctggacaaa ttatagaaaa acataaaacc ggaaaagaaa tagatccctt
       61 ccagaacaaa atttgccatc atcactttca ctaaagtccg cgcatctttt tttacctgaa
      121 attcattata gcattcccct ataaatgtat tccttttcaa cagatgttcg tctgttttcc
      181 actggtataa aatatccatt ctttcatcag gcgagcagat cgtatcaagc atataagaat
      241 atgactgtga atgtacacat tcctgaaacg tctgaatgtt cagacagaga ttcacttcat
      301 ttgccgtaat atattctccg acgcagggca gatttgccgt ctgaatcgaa tcaagaaata
      361 cgagaaagga caaaatttta tcataggctt tccgctccgc cggcgataga ttcgggtaat
      421 cttttatatc ctgcgacaag ttgatttcct ccggtatcca gaaattattc attgcctgac
      481 gataccattc ggatgcccat gtatatttca aattattata atcatttaaa ttagttgtat
      541 tcccgccgat catgcggcgt ttcagtacgt ccgtatctcc gtccggatta aaaagcggtt
      601 tgtgcggaag cggttttttt atatcaaatt gctgcatagt cgtctccttt ttctttttgc
      661 cggcagcaaa aagcgacttc ttgttttcta ggaagaacaa gactcacact cttctacttc
      721 cagcgatttt gaccttacat agtatactgt ttttacgccg cattcccatg cctttaaata
      781 caggttaagc acttggcgca ttgtataatc gtttgttata taaaaattca tcgactgagc
      841 ctgatccaca tgtctttgtc tgactccgca tgcgcgtaca gaccaattct gatcaatatg
      901 atgtgcgttt atgtaatacc aatacgtctc cgggctaaga tcaggcgcaa ctctgggaag
      961 catagatccc tttttctctt ccaggaaata cttgttcata accggatcga tccccgctgt
     1021 cgttcccgcg ataatggaag tggaacttgt cggcgcgatc gccaaaagat atgcgtttct
     1081 cattccctgt ctgtgtactt tttcccttag ctccttccac tcatccgagc agtatccccg
     1141 cttttcaaaa taggctccgc tctcccagtc cgatccttca aaatatcggt aagaaccttt
     1201 ttctttcgca taatcactgc ttgcttcaat ggccgcataa ttaatctgct caaacaattt
     1261 atcaacaaac gccaaatgtt cttccgactg ccatttgatc ttatttttcg caagcatatg
     1321 gtgatatccg cttaccccaa gacctataga tcgatatgta tggtttgtga tctttgcgta
     1381 gggaacagga taaaaattca gatcgatcac gttgtcaagc gccctgacaa ccgtacgcac
     1441 aactttacgg acatatgcag gatcatttac cggaaggtta ccaagtgaca aagacgccaa
     1501 attacagacg acaaactctc ctgcttttgt tgtattgacg actactgtgt ctttgtcttc
     1561 tgtctgtatt tttgtttcaa ccgtagaaat cgcggacata ttctgcgcaa tttccgtaca
     1621 aagattagaa caataaatca ttcctttgtg agggtttgga ttcgcacggt tcactgtatc
     1681 tctgttaaat gcaaacggag ttccggtctc cgccgctgat ttgatgatca gacggatcag
     1741 atctttcaaa agaactgtcc ttttcttaat tcttgcatca tgaacacaat cccaatagcg
     1801 gcgctcccat tcttctccga aactatcttc caaacaatat ccttttaccg tcatgatctc
     1861 atgcgggcac atcagatacc agtcctgatc caggttttct cctgccattt tccagaacag
     1921 atccggataa cagactgccg gaaatacatc gtgcgctttc attctctcat cgccgttatt
     1981 tgtccgaagc tgaaggaatt ccggcaagtc tctgtgccat gcatcgagat ataccgcgac
     2041 cgctcccgct cttaccccaa gctgatctac cgcaaccgca gtatcattca cgatgcggac
     2101 ccagcgtatc actccgcctg ccgccccttc aaatcctcgt atcatacttc ccgatgcacg
     2161 tactttcccg aaatacaacc ccattccgcc gccgtatttg cttaccatgg caaaattatc
     2221 aatcgaccgg aaaattcctt tcaaactgtc gggaacagtg tcaataaaac aagaactaag
     2281 ctgatgaaac ggctttcttg catttgccag agtcggagtc gccattgtca cttctaattt
     2341 agagagcata tcgtaaaact ctctcaccca cataagtctg atctttcttt tctcattcat
     2401 cgcaagatga agggcaatcc ctaagaacat ctcctgcgga ctctccagcg gaacatgccg
     2461 atgtgttgag atcacatatc tctttatgag aagatccaat cccgaatagt taaacagcct
     2521 gtttctttct ttgcatatat aactctccgc ttctacgatc tcttcttttg agtaattttc
     2581 catgatataa gaaccgtaca gtcctttttc tgtcaggtaa acaatctttt catacagatc
     2641 tgaaatcccg atcgatttct cttcttcttt caatttttcc gaaaagcgaa aatcaagcag
     2701 ccatcctgcg atcatttccc aacgcggcgc ttcctgactt gtaagttccg ccgccgcttt
     2761 gatcagcgcg tcgaatcttt ccccatcgtc catatccgtt ttcaaaaatc ccgaaaattt
     2821 ctcaaaaaga agttccaatg aataaacttt ttccggaaat tctgtgtgta tgtcatataa
     2881 aagcttttcc aaccgttctt gaaactgcaa agtacggcac acaaagctgt ctgttatcaa
     2941 agtcattcct tttttctcaa atgtttctgt catcaattcc tccttttttc ttcctgtatc
     3001 ttttcctcat tttcatgaag acctaaaacg ccttctacaa gaatctcaac atcattaaat
     3061 atataatcaa atgctcctgc ctgacacata ttgatcaaaa ccattccgac cggcactgac
     3121 aagatcatcc agagtaatcc tcctgcttta tagcctacat acaaaagcag cagggtaaca
     3181 agcggattca gtccgacact gtcgccaacc attttcggct gaaggagctg tcttacaacc
     3241 tgtgtgatca catagaggac gaccaaagat accgtcatct tgtaatcacc cataaaaaac
     3301 tgatacacag cccacggtat catcgctgtt ccggttccga aaaacggaag gaaatcaaga
     3361 aatgagatca aaaactagcc cccatctcaa tcatatacca aatctctccc tcactaaacg
     3421 taagc
//
LOCUS       DBMDXF010000194         3419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6279_length_3419_cov_2.052441, whole genome shotgun sequence.
ACCESSION   DBMDXF010000194 DBMDXF010000000
VERSION     DBMDXF010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6279_length_3419_cov_2.052441"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="ACZPD4_07450"
     CDS             complement(<1..550)
                     /locus_tag="ACZPD4_07450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR1378923.1"
                     /translation="MTPIIFHIDVNSAYLSWTAVEQLKNGAKVDIREIPAIIGGDQTS
                     RHGVVLAKSTFAKKYGIRTGEPVANAFRKCPNLVMYPPDHRMYREKSRQMMEYLKTFT
                     KEIEQVSVDECYMDFTKIAARYSSPIDGALEIKEGIKKKFGFTVNVGISTNKLLAKMA
                     SDFEKPDRIHTLLPEEIEVKMWP"
     gene            complement(618..3230)
                     /gene="clpB"
                     /locus_tag="ACZPD4_07455"
     CDS             complement(618..3230)
                     /gene="clpB"
                     /locus_tag="ACZPD4_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR1378924.1"
                     /translation="MNINRFTQNSIQAVQNCEKIAADNGNQELAQEHLLYALLTQDDS
                     LILKLFEKMNIQGQLLINRVDQAIGKRPKVQGGQLFVGQDLNNVLIHAEDEAKQMGDE
                     YVSVEHLFLSLLKYASKEMKQIYREFGISREGFLHALSTVRGNQRVTSDHPEATYDTL
                     NKYGQDLVERARDQKLDPVIGRDEEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGL
                     AQRIVSGDVPEGLKDKTIFSLDMGALVAGAKYRGEFEERLKAVLEEVKNSDGKIILFI
                     DELHTIVGAGKSDGAMDAGNMLKPMLARGELHCIGATTLDEYRQYIEKDAALERRFQP
                     VLVDEPTVEDAISILRGLKERYEVFHGVKITDSALVAAAMLSNRYISDRFLPDKAIDL
                     VDEACALIKTELDSMPTELDELRRRIMQLEIEEEALKKEEDRLSRERLEHLQEELAGL
                     KEEYAGEKVQWENEKHSVERVQKIREEIEHVNKEISKAQREYDLNKAAQLQYGELPQL
                     QKQLEEEEEKVREKELSLVHEAVTDEEIARIVSRWTGIPVAKLNESERNKTLHLADEL
                     HKRVIGQDEGVELVTEAIIRSKAGIKDPSKPIGSFLFLGPTGVGKTELAKALAQSLFD
                     DENNMVRIDMSEYMEKYSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEKA
                     HPDVFNVLLQVLDDGRITDSQGRTVDFKNTILIMTSNIGAGYLLDGIKEDGSISKEAQ
                     DMAMNDLRAHFRPEFLNRLDEIIMFKPLTKENIRAIIDLLVADVNRRLEEKELTIELT
                     EAAKDCIVEGGYDPMYGARPLKRYLQKNVETLAARLILAGNVGREDTILIDAKDGKLK
                     ADVKRVNSLERQEA"
BASE COUNT          771 a          933 c          609 g         1106 t
ORIGIN      
        1 gcggccacat tttcacttca atctcttccg gcaataatgt gtgtatccgg tccggctttt
       61 caaaatctga cgccattttc gccagtaatt tattcgttga gattccgaca tttaccgtaa
      121 atccaaactt ctttttaatc ccttccttaa tctcaagcgc tccgtcgatc ggagaactgt
      181 accgcgcggc gatcttcgta aaatccatat aacactcgtc tacgctgacc tgctctatct
      241 ctttcgtgaa tgtctttaaa tattccatca tctgtctgct cttctcgcgg tacatccgat
      301 gatcaggcgg atacattacg agattcggac acttacgaaa ggcattcgca acaggttctc
      361 ccgtgcggat gccatatttt ttggcaaaag tggattttgc gagaacaaca ccgtgccggg
      421 atgtctgatc cccgccgata attgccggga tctcccgaat atccactttc gccccgtttt
      481 ttaattgttc tacagccgtc cagctcaaat aagctgagtt tacatcaata tgaaagatta
      541 tcggtgtcac ttgcgccatc ctttcacaca ccggcttcat gcagactctt ttcttctcgc
      601 ttttttgcat atcttactta tgcttcctgt ctttccaagc tgtttacccg cttcacatct
      661 gctttcagtt tgccgtcttt cgcatcgatc agtatcgtgt cttctcttcc gacatttccg
      721 gcaagaatca accgtgcagc cagtgtctct acgtttttct gcagatatct ctttaacggt
      781 cttgctccgt acatcggatc atatccgcct tctacgatac agtctttcgc cgcttccgtc
      841 agttcgatcg tcagttcttt ttcctccagc cgtctgttta catctgccac taacaagtca
      901 ataatggcac gaatattttc tttcgtcaat ggtttaaaca taatgatctc gtcaagacgg
      961 ttcaaaaatt ccggtctgaa gtgagctctt aaatcattca tcgccatatc ctgcgcctct
     1021 ttcgagatcg aaccgtcttc ttttataccg tctaaaagat aacctgctcc gatattggac
     1081 gtcatgatca gaatcgtatt tttgaaatca acggtacggc cctgagaatc tgtaatcctt
     1141 ccatcgtcaa gcacttgcaa aagcacgtta aatacatccg gatgcgcctt ctctacttcg
     1201 tcaaataata cgactgaata cggttttctt ctgactgcct ctgtcagttg accgccttcg
     1261 tcatatccta catatcccgg aggcgctccg atcagtctcg acacggaata tttctccatg
     1321 tattcgctca tatcaatccg caccatatta ttctcatcgt caaacaggct ctgcgccaac
     1381 gccttcgcaa gttctgtttt tcccacgcct gtaggtccga ggaagaggaa cgatccgatc
     1441 ggcttgctcg ggtctttgat tccggctttg gaacggatga tcgcctctgt tacaagttcg
     1501 actccttcat cctgacctat gactctttta tgaagttcat cagcaagatg aagtgttttg
     1561 tttctttcgc tttcatttaa tttcgctacg ggaattcccg tccatcttga aacaatcctt
     1621 gcgatttctt cgtctgttac cgcttcatga acaagagaca attctttttc tcttactttt
     1681 tcttcctctt cttcaagctg tttttgcagc tgaggcagtt caccatactg cagctgcgcg
     1741 gctttattca gatcatactc tctttgcgct ttgctgatct ccttattcac atgttcgatc
     1801 tcttcacgaa ttttctgtac ccgctcaaca gaatgctttt cattctccca ctgtaccttt
     1861 tcaccggcat attcttcttt cagacccgcc agttcttcct gtaaatgttc cagacgttca
     1921 cggcttaatc tgtcttcttc ttttttcagc gcctcttctt caatctcaag ctgcataatc
     1981 cttcttctta attcgtccaa ctctgtcggc atcgaatcaa gttctgtctt aatgagcgca
     2041 caagcttcat ctacaaggtc tatcgctttg tccggcaaaa aacgatccga aatatatctg
     2101 ttcgacaaca ttgccgctgc gacaagagcg ctgtctgtaa tcttcactcc atgaaatact
     2161 tcataacgtt ctttcagacc acgcagaatc gaaatggcgt cttctactgt aggctcgtca
     2221 actaaaaccg gctggaaacg gcgttccaaa gccgcatctt tctcaatata ttgacgatac
     2281 tcatcaagcg tcgtcgctcc gatacagtga agttcacctc ttgcaagcat cggtttcagc
     2341 atatttccgg catccatagc gccgtccgac tttcctgctc cgacgatcgt atgcagctca
     2401 tcgataaaca gaataatctt tccgtcactg tttttcactt cttcaagaac tgctttcagt
     2461 cgttcctcaa actcgccccg atactttgct cctgcaacga gcgctcccat atcaagcgaa
     2521 aagatcgtct tatctttcaa accttccgga acgtcgccac tgacgatccg ctgggcaagt
     2581 ccttctacaa ccgctgtttt tccgactccc ggctctccga taagcaccgg attgtttttc
     2641 gtcttacggg aaagaatccg gatcacattt ctgatttcct cgtcacggcc gatcaccgga
     2701 tcaagttttt gatccctcgc ccgctctacc agatcctgac cgtacttatt cagcgtatca
     2761 taagtcgctt ccggatggtc gcttgtcact ctctgattgc ctctgacagt agacagcgca
     2821 tgtaaaaatc cttcccggct gatgccgaat tcacgataaa tctgtttcat ctctttgctt
     2881 gcatatttta acagggaaag gaaaaggtgc tctacggaaa catattcgtc tcccatctgt
     2941 ttcgcctcgt cctcggcatg gatcaacaca ttgttcagat cctgccctac gaaaagctga
     3001 ccgccctgaa ctttcggacg ctttccgatc gcctgatcta cccggtttat caaaagctga
     3061 ccttgtatat tcatcttttc aaacaatttt aagatcagac tgtcatcttg tgtaagcagc
     3121 gcatacaata aatgttcctg cgccaattcc tgatttccgt tatctgcggc gatcttctca
     3181 caattttgca ccgcctgaat ggaattctgt gtaaatctgt ttatattcat gctaatccct
     3241 cctttatttt cgtgttctac taggaatata gcatctgttg ttagcactgt caagggttga
     3301 gtgctaatta ttttgtgaag aatttgtgaa ccttaaatga aaaactaact tccaccttta
     3361 gtctgttttg ctctacatta ggaactgggg gtggaagtta gtcttgcaat cgtttattc
//
LOCUS       DBMDXF010000195         3379 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6366_length_3379_cov_1.958206, whole genome shotgun sequence.
ACCESSION   DBMDXF010000195 DBMDXF010000000
VERSION     DBMDXF010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_3379_cov_1.958206"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(213..1718)
                     /locus_tag="ACZPD4_07460"
     CDS             complement(213..1718)
                     /locus_tag="ACZPD4_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR1378925.1"
                     /translation="MGMNRKTGRGAKFLIVFVVIVIIMAAVTFFAGKYAYHLLREYIE
                     YASKQSTEVVLEKDGLKGMIEWMSEKEKEKLPKKFLVSDIEAELWKNGEVYDFAFNIQ
                     EFDESDEYVKDIYYRYDSREGKLSKTENVNEAFPTEYDPNAEVDYLDSQIKMLPLMAQ
                     MKELDFDRYVVEYSQDRRLQDADVVIDGRDGNGFSVLTQKEYQQGAGGASDGSSQVVI
                     SLTDGGGVMGERIEYICALADENALVGQTETVMQTDYYFRGEELMLTDDSGETWVASG
                     LTTKQLEETKAVYGQGNMIPENSVYADGNGMFAVFWGETPTLHVSKDDGETWTDFVFQ
                     EEYPRLCTSRIVRFLDPENGYVGLGTDWSMGTGGATYIGWTHDGGATWETTPVAVENG
                     WILSGLAFADQSAGMLTMDEQFGENSWPHVLVTENGGASLAEIELPWDTVSEEVMFLN
                     KVDSLKYENGVYYLTLGQGEYGNKKADFTSTDLKSGWKFEKSYIGTVHLNG"
     gene            complement(1800..3089)
                     /locus_tag="ACZPD4_07465"
     CDS             complement(1800..3089)
                     /locus_tag="ACZPD4_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1378926.1"
                     /translation="MQERKNSEAGNEKEVDYGRKSGQDNTSENVIENRPEKRVKNKGG
                     NKTGEFIARKRKERGMTQKEMAELLGVTNKAVSKWETSQGMPDIGILPELGKALGVTV
                     DEILMGEQIEQEKRAETAVSDEDKKLLEIVLERAERKAETIRITWKDVFGCLLILSAA
                     GLIIVQIWTLTQGRELGLIYIRNVTPYVINAAAVFLFWAGGMCIEKLRPIWKRKSVIA
                     VTAILLAAGIAVCPFCFPRQREIVDLAPDFSNTMCLKIDENGRAVFYRHRGLLFGAQS
                     DVFPFTVKDDVKVQWLENDVCALTYESPEDDQVHQFVATYGDRNKAVSYYYVVNAAYG
                     TWRAKDQGENYKLEVGTGENGGIDIETPEGKEHYEPEKCLQYGTLAVVFPSDDPKWTL
                     VLNKDCVVEAGGSRIEEGGTVTLCKIAMEKTAPIIMH"
BASE COUNT          887 a          931 c          474 g         1087 t
ORIGIN      
        1 aaacaagatt acttaaataa cgttgcaacc atctactcag aatcagaaca cgaaacagaa
       61 cacttgaaca gctgcaatga gtaccttcaa aacctccaat ctgatttagc agaaaaagaa
      121 gattatgaaa taatggaata aaaatgttta ataacaactt aaaatatcgc atcttaaacc
      181 tgtcatttaa gatgcgatat ttttatgccc ccctatccat tcaaatgaac tgttcctata
      241 tagctttttt caaatttcca tccgctcttc aaatctgtac ttgtaaaatc agctttttta
      301 tttccatact ccccctgacc gagggttaag taataaactc cattctcata tttcaaactg
      361 tccactttat tcagaaacat cacttcttcc gaaacagtat cccacggaag ctctatttcc
      421 gcaagtgacg ccccgccgtt ttctgtcaca agtacatgcg gccacgaatt ttctccaaat
      481 tgttcatcca tcgtaagcat cccggctgac tgatcggcaa atgcaagtcc cgacaaaatc
      541 catccgtttt ccacagctac aggtgttgtc tcccaagttg cgccgccgtc atgcgtccag
      601 ccgatgtaag tagctcctcc ggttcccatt gaccagtctg ttccaagacc tacatatccg
      661 ttttcaggat caagaaaacg aacaattctc gaagtgcata gtctcggata ctcctcctga
      721 aatacaaaat cagtccaagt ctcaccgtca tcttttgaga catgaagcgt cggggtctcg
      781 ccccaaaata ctgcaaacat tccgtttccg tctgcgtaaa cactgttctc cggaatcata
      841 tttccttgac cgtataccgc tttcgtttct tccaactgct ttgtcgtcaa accgcttgca
      901 acccatgttt ccccactgtc atctgtcagc ataagttcct ctcccctgaa ataataatcg
      961 gtctgcatca ctgtctccgt ctgaccgaca agcgcatttt catctgccag agcacagata
     1021 tattcgatcc tctctcccat cactcctcca ccgtcagtca gagagatcac aacctgcgaa
     1081 cttccgtcac ttgcccctcc tgctccctgc tgatattctt tctgtgtcag cacggaaaat
     1141 ccgtttccgt ctcttccgtc tatcactaca tccgcatctt gaagccgtct gtcctgactg
     1201 tattccacga catatctgtc aaaatccaac tccttcatct gcgccattag cgggagcatt
     1261 tttatctggc tgtccaaata atccacttct gcattcgggt catactcagt cggaaacgcc
     1321 tcgtttacat tttctgtttt actaagcttt ccttctctgc tgtcatacct gtaataaata
     1381 tctttcacgt attcatctga ttcatcaaat tcttgaatat taaacgcaaa gtcgtacact
     1441 tctccgtttt tccacaactc tgcctcgata tcggaaacaa gaaatttttt cggaagtttc
     1501 tctttttctt tctcagacat ccattcgatc attcctttca gtccgtcttt ttctaaaacc
     1561 acttccgtac tttgtttcga cgcatattct atgtactccc ttaacaaatg ataagcatac
     1621 tttcctgcaa aaaatgtaac tgctgccatt atgatcacta tgacaacaaa cacaataaga
     1681 aattttgcgc ctcgtcctgt ttttctgttc attcccatat tttctcctct ctattctaag
     1741 cgctccgaat ctgatttact ctttttgacg ttctatcctt tcattccaaa tggatttctc
     1801 taatgcataa tgatcggggc agttttttcc atcgctattt tacacaaagt aactgttccg
     1861 ccttcttcta tccggcttcc tcccgcttcc actacgcagt ctttatttaa cacaagcgtc
     1921 catttcggat catcactggg aaatacaact gcaagcgttc catactgaag acatttttcc
     1981 ggttcataat gctctttacc ttccggtgtt tcaatatcga ttccgccgtt ttctcctgtt
     2041 cctacttcca acttataatt ttctccctga tctttcgccc gccatgtacc gtaagccgca
     2101 tttactacat aataatatga taccgctttg tttctgtctc cgtaagtcgc cacaaactga
     2161 tgcacctgat catcttccgg cgactcatat gtaagagcac acacatcgtt ttcaagccac
     2221 tgtactttca catcatcttt cacagtaaac ggaaatacat cagactgcgc tccgaacagc
     2281 agcccccgat gccgataaaa aaccgctctt ccgttttcat caattttcag acacattgta
     2341 tttgaaaaat caggtgcaag atcaacgatt tccctctgcc ttggaaaaca aaatggacaa
     2401 accgcaattc ctgcagctaa taaaatcgcc gttactgcaa taacagattt tcttttccat
     2461 atcgggcgga gtttttcaat gcacatgccg ccggcccaaa aaaggaaaac tgccgccgcg
     2521 tttatcacat atggagttac attccttata tagatcaatc caagttctct tccctgagtc
     2581 agagtccaaa tctgcactat gatcaacccc gccgcagaca agattagcag acagccaaaa
     2641 acatctttcc atgtgatccg aatcgtttcc gcctttcttt cggctcgctc caacacaatt
     2701 tccaaaagct tcttatcctc atccgatacg gcagtttccg ctcttttctc ttgttcaatc
     2761 tgttctccca ttaaaatttc atcaactgtc actccaagcg cctttccaag ttcaggaagg
     2821 attccgatat ccggcattcc ctgacttgtc tcccattttg acacagcctt attcgtaacc
     2881 ccaagcagct ctgccatttc cttctgagtc attccgcgtt cctttctctt tcgcgcaata
     2941 aattctcctg tcttatttcc tcctttattt tttactcttt tctccggtct gttttctatt
     3001 acattttccg atgtattatc ctgtccgctt tttctcccat agtcaacttc tttttcattt
     3061 cctgcttcac tgttttttct ttcttgcatt ttgttcctcc ttccgcatcg tctcgcagaa
     3121 agtaaaacat tttactgacc taatcatatc aaactatgaa aattttacaa tacacgttca
     3181 ttttccatcg gtctacgctg cgtagactcc ctaaatcaaa cgtaaatcat tcggttttca
     3241 taaaattttg agaatccccc ggctgatcat acaaaaacag acaagaaaaa acagaataca
     3301 aatacaaatt taacactgtc aataacttat gaaaatgtca ctttttaata tagaaattta
     3361 tttgaaaaaa tgtcacttt
//
LOCUS       DBMDXF010000196         3305 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6527_length_3305_cov_1.695381, whole genome shotgun sequence.
ACCESSION   DBMDXF010000196 DBMDXF010000000
VERSION     DBMDXF010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6527_length_3305_cov_1.695381"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="ACZPD4_07470"
     CDS             <1..734
                     /locus_tag="ACZPD4_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR1378927.1"
                     /translation="AEVIPLTTGTATLKDAVSEAMREWTSRIADTHYCLGSVMGPHPF
                     PTIVRDFQAVISKEIKEQILQKEGRLPDAVIACVGGGSNAIGSFYHFIEDKKVRLIGC
                     EAAGRGIDTDETAATITTGRLGIFHGMKSYFCQDEYGQIAPVYSISAGLDYPGVGPEH
                     AYLHDMGRAEYVPVTDEEAVWAFEYLAKTEGIIPAIESAHAVAHAMKVAPLMSPEKII
                     VVTISGRGDKDCAAIARYRGEDIYE"
     gene            727..1503
                     /gene="trpA"
                     /locus_tag="ACZPD4_07475"
     CDS             727..1503
                     /gene="trpA"
                     /locus_tag="ACZPD4_07475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR1378928.1"
                     /translation="MSNIKNAFKNKKAFIAFFTCGDPDLETTKKAIRSAVKNGADLIE
                     LGIPFSDPTAEGPVIQAANLRALKSGITTDKIFAFVRELRKDIDVPFVFMTYTNVVFS
                     YGVEKFVSECQKSRIDGLILPDLPFEEKEEFHPICAGYGVDLISMVAPTSEKRIAKIA
                     GEAEGFLYVVSSLGVTGMRSEITTDLTSIVATIRENTRIPCAVGFGISTPQQAAAMAE
                     YADGVIVGSAIVNIMEKYGKDAPQYVGDYVKEMKKALSVY"
     gene            1675..2349
                     /locus_tag="ACZPD4_07480"
     CDS             1675..2349
                     /locus_tag="ACZPD4_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1378929.1"
                     /translation="MRLLLAEDERALSKALTAILERNNYSVDAVYDGQSALDYLEADN
                     YDGVVLDIMMPKVDGLTVLRKVRERGNLIPILLLTAKSEVDDKVEGLDAGANDYLAKP
                     FHSKELLARIRAITRAQTAQANSKLKMGNVTLDRAAFELSTSKGSFRLANKEFQMLEL
                     LMCNPHQLISSERFLEKIWGYDSEAEINVVWVYISYLRKKLSALHADIQIKATRNAGY
                     SLEERS"
     gene            2346..>3305
                     /locus_tag="ACZPD4_07485"
     CDS             2346..>3305
                     /locus_tag="ACZPD4_07485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378930.1"
                     /translation="MIKKLRIKLIVASMISLFLVLFIIGGIAGVLNYRKIAEDADRIL
                     EVLEKNAGHFPKMIPGNEKGDRHGISPEIPYESRYFSVLLDEKGEVILTDTSKIVSID
                     TENAIEYASEVWARGKEKGFMDHYRYWKTSYGGEIRVIFLDCRRQLDNFLNFLFTTMI
                     VSFIGLLSVLFLMIYFSGRIVKPFSDNYEKQKRFITDAGHELRTPLTIIEADTEVLEM
                     DFDENEWLQDIREQTKRLADLTGSLVMLSRMEEGQNGNLKVEFPLSDMVEEVCHTFQA
                     PAKIQGICMKTAITPMISIKGDEKAIRSLITILLDNAVNVMTTI"
BASE COUNT          994 a          548 c          879 g          884 t
ORIGIN      
        1 gagcggaagt gatcccttta acgacaggga cggcaacttt aaaagatgcc gtttcggaag
       61 cgatgcgtga atggacctcc cggatcgccg atacgcacta ctgtctcgga tcggttatgg
      121 gaccccatcc gtttcctacg attgtccgtg attttcaggc agttatttca aaagagatca
      181 aagagcagat ccttcaaaaa gaagggcggc ttccggatgc ggtgattgca tgtgtcggag
      241 gcggttcaaa tgcgatcggc agcttttatc attttattga ggacaaaaaa gtccgcctga
      301 tcggctgtga agcggcaggc aggggaattg ataccgatga aaccgcggct acgatcacga
      361 cagggagact tgggattttt catggtatga aatcttattt ttgccaggat gaatatgggc
      421 agatcgctcc ggtatattct atttccgccg gactggatta tccgggagta ggcccggaac
      481 atgcctatct gcatgatatg ggaagagcgg aatatgtgcc ggtgacggat gaagaagccg
      541 tttgggcgtt tgaatatctg gcaaagacag aagggatcat tccggcgatc gaatcggcgc
      601 atgcagttgc tcatgcaatg aaagtcgcgc cgttgatgag tccggaaaag ataatcgtag
      661 ttacgatttc cggcagagga gataaagact gtgcggcgat cgcccgatac agaggggagg
      721 atatttatga gtaatatcaa gaatgcgttt aagaacaaaa aagcatttat tgcatttttc
      781 acctgtggag atccggatct ggaaacaacg aaaaaagcga tccgatcagc agtaaaaaac
      841 ggtgcggatc tgatagagct tgggattccg ttctctgatc cgaccgcgga aggcccggtt
      901 attcaggcgg caaatttaag agccttgaaa agcgggatca ctaccgataa aatttttgcg
      961 ttcgtaagag aactgcgcaa agacatagat gtaccgtttg tttttatgac gtatacgaat
     1021 gtggtatttt cttatggtgt agaaaaattt gtttcagagt gtcaaaagag ccggattgac
     1081 ggactgatcc tgccggatct tccatttgaa gaaaaggaag aatttcaccc gatctgtgcc
     1141 ggatatgggg tggatctgat ctctatggtt gctccgactt ctgaaaagcg gatcgcaaag
     1201 atagcaggag aggcggaagg atttttgtat gttgtctcga gcctcggcgt gacggggatg
     1261 agaagtgaga ttacgacaga cctgacttcc attgtggcga ccattcgtga aaacacccgg
     1321 attccgtgtg cggtcggctt tggaatctcc acgcctcaac aggcagccgc gatggcagaa
     1381 tatgcggatg gagtgatcgt aggatctgcg atcgtaaata taatggaaaa gtatgggaag
     1441 gatgcgccgc aatatgtcgg cgattatgtg aaagagatga aaaaagcgct gtctgtatat
     1501 tgatatagag ggcagaaaaa ctggaaaata tgtttttcct aagatatact atgtcatagt
     1561 aacagcattt taaagtagga ttaaggtgat acgtttattt ttaaatagaa atatagttcg
     1621 ctgtgagtaa aggaaaaaga taagtgaagc gggcggaaac aggagatata tattatgcgg
     1681 ttattattgg cagaagatga aagagcgctg tcaaaagcgc tgacagcgat tttggaaaga
     1741 aataattact cagtggatgc agtttatgac ggtcagagcg cattggacta tctggaagcg
     1801 gataattatg atggggtagt cttagacatt atgatgccta aagtggatgg actgacagtg
     1861 ttaaggaaag ttcgggaaag ggggaatctg attccgattc tgttgttgac agccaaatca
     1921 gaagtggatg ataaagtaga gggattggat gccggagcaa atgattatct ggcaaaacct
     1981 tttcattcaa aagaactgct ggcaagaatc cgggcaatca caagagcgca gacggcacag
     2041 gcaaattcca aattgaagat gggaaatgtc acgctggatc gggcggcatt tgaactttct
     2101 acaagtaagg gaagtttccg ccttgccaat aaagaatttc agatgttgga gcttttaatg
     2161 tgcaatccgc atcaattaat ttcttctgaa cggtttttgg aaaaaatatg gggatatgac
     2221 agtgaagcag agataaatgt agtttgggta tatatttctt atttgaggaa gaagttgtct
     2281 gctctccacg cagatattca gatcaaagcg acgagaaatg caggatattc tctggaggag
     2341 cggtcatgat aaaaaaactt cgtattaaac tcatcgtagc gtccatgata tctttgtttc
     2401 ttgtactttt tatcatagga gggattgcag gtgttttaaa ctacaggaag atcgcagaag
     2461 atgctgatcg aattcttgaa gttttggaaa aaaatgcagg acattttccg aaaatgattc
     2521 cgggaaacga aaaaggggac agacatggga tctcaccgga gatcccttat gaatccagat
     2581 atttttctgt tctattggat gaaaaaggag aggttatttt aacagatacg tcaaagattg
     2641 tatccataga cacagaaaat gcaatagaat acgcatcaga agtttgggca agaggaaaag
     2701 aaaaaggatt tatggatcat tatcgctatt ggaagacgtc ttatggcggg gagatacgtg
     2761 ttattttctt agattgcagg agacagctgg ataatttcct gaactttttg tttactacaa
     2821 tgatcgtatc tttcataggg ctattatcgg ttttgttttt gatgatttat ttttccgggc
     2881 gtattgtcaa accgttttct gataattatg aaaagcagaa gcggtttatt acggatgcgg
     2941 ggcatgaatt aaggacaccc ttgacgatta ttgaagcaga cacggaggtt ttggaaatgg
     3001 acttcgatga gaatgaatgg ctgcaggata ttcgggaaca gacaaaaaga cttgcggatc
     3061 ttacaggttc tttagttatg ctttcccgta tggaagaagg gcagaatgga aatttgaaag
     3121 tagagtttcc tttatctgat atggtggaag aggtatgtca tacttttcag gcgccggcga
     3181 agatacaggg aatctgtatg aaaactgcga ttacacctat gatttctata aaaggcgatg
     3241 aaaaagcaat ccgaagtctg ataacgattt tgcttgataa tgccgtcaat gtgatgacaa
     3301 caata
//
LOCUS       DBMDXF010000197         3302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6534_length_3302_cov_1.635114, whole genome shotgun sequence.
ACCESSION   DBMDXF010000197 DBMDXF010000000
VERSION     DBMDXF010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6534_length_3302_cov_1.635114"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /gene="tig"
                     /locus_tag="ACZPD4_07490"
     CDS             complement(<1..878)
                     /gene="tig"
                     /locus_tag="ACZPD4_07490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1378931.1"
                     /translation="MSLQVERLEHNMAKLTIEVPAEEVEKALQAAYLKERGKISLPGF
                     RKGRVPRQMIEKMYGPEVFYDEAANRMISEAYAKAYDECELELVSQPKIEITQLEKGK
                     EFIFTAEVAVKPEVKLGEYKGLKVDKVSTRVTQKEVDEEIDKERERNARTIDVTDRAV
                     QDKDQITLDFEGFVDGVAFEGGKASDYPLTIGSGAFIPGFEDQLIGANIDEEKEINVT
                     FPEEYQAKELAGKAAVFKCTVHSIKAKELPELDDEFVSDVSEKSETVDAYKAEVKAKI
                     KERKENEGKQKREDQSV"
     gene            complement(1059..1691)
                     /locus_tag="ACZPD4_07495"
     CDS             complement(1059..1691)
                     /locus_tag="ACZPD4_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIR1378932.1"
                     /translation="MNSPLILSYNDYSRANAEKKEKVKYGGSNLKVYIFLADGFEEIE
                     GLTVVDIMRRAKIDVETISITGQKQINGAHKIIVEADRLFEETDFSDGDMLVLPGGMP
                     GTLNLKEHEGLRNLIGEFDKKKKYLAAICAAPSILSELGILKGRKACAYPSFEEGLDC
                     AQVVHEAAVTDGHVTTGRGMGAAIPFALKLTELLCGTEKANEIAESIVYA"
     gene            1793..2245
                     /locus_tag="ACZPD4_07500"
     CDS             1793..2245
                     /locus_tag="ACZPD4_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1378933.1"
                     /translation="MEIERKFLVDLSDLPFDPKDYPARKIEQAYLCTAPVVRIRRDND
                     DFFLTYKSKGMMVREEYNLPLTKESYYHLLLKADGRIITKTRYVIPIENGLFVELDIF
                     EGDLAPLVLAEIEFPDEAFAKAYQAPSWLKEDVTYSSEYHNSTLSKKD"
     gene            2472..>3302
                     /gene="proB"
                     /locus_tag="ACZPD4_07505"
     CDS             2472..>3302
                     /gene="proB"
                     /locus_tag="ACZPD4_07505"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1378934.1"
                     /translation="MGLCNNRIVVKVGTSTLTNDAGKSDLRAIDRLVCTLADIQNMGY
                     EVILVSSGAIAVGRNKLNMHEKPDSMRMKQAAAAVGQCSLMYLYDKFFGDYDKTVAQI
                     LLNAEDIEQEEKKENLTNTFDALLEMGVIPIVNENDSVSYKEIESEDRLFGDNDMLSS
                     VVSVLCRAKRLVIFSDIDGFYDSDPRLHPNAKLIKEIRQIDESVYSLAGGAGSRRGTG
                     GMRTKLQAAELAASQGIDTIVTNGKHPEALYDIVNGKQAGTLFVGNRGEEGIWCYCQC
                     L"
BASE COUNT          864 a          833 c          622 g          983 t
ORIGIN      
        1 acagactggt cttctctctt ttgctttcct tcgttttctt tgcgctcttt gatcttcgct
       61 tttacttctg ccttatatgc gtcaacagtc tcgctcttct cagatacgtc ggatacaaac
      121 tcatcatcga gttccggaag ttcttttgcc ttgattgaat gcactgtaca tttgaatacg
      181 gcagcttttc cggcaagttc tttcgcctga tactcttcag ggaatgtcac attgatctct
      241 ttttcttcgt caatgtttgc tccgatcaac tgatcttcaa atcccggaat aaacgctccg
      301 gatccgatcg tcaacggata atcggaagct tttccgcctt caaaagcaac tccgtcgaca
      361 aaaccttcaa agtcaagcgt gatctgatct ttatcctgaa ccgctctgtc agttacatcg
      421 atcgttcttg cattgcgttc acgctctttg tcgatctcct catctacttc tttctgtgta
      481 actcttgtag aaactttatc aactttcaga cctttatatt caccgagttt tacttccggt
      541 tttacagcca cctctgccgt aaagataaat tctttacctt tctcaagctg tgtgatctcg
      601 atcttcggct gtgacacaag ctcaagctca cactcgtcat atgctttcgc atacgcttct
      661 gagatcatgc ggtttgccgc ctcatcatag aacacttccg gtccgtacat cttttcgatc
      721 atctgacgcg gaactcttcc tttacggaat cccggaagac tgatcttgcc gcgctctttc
      781 agataagctg cctgaagcgc tttttccact tcctctgccg gtacttcgat cgttaatttt
      841 gccatattat gttctaatct ttctacctgt aaactcattt ttttcctcct tgaatgtgct
      901 gacttcaaaa ctgcctcttc gtcatatcgc acttataaaa aaatatacac tcgcagtcat
      961 tgtcatagta tagcatacac atccgcatat ttccagtatt ttttcgtcca agttcagaca
     1021 tacatgaaac acacgggaaa aatatttttg cagactgtct atgcatatac gatagattcc
     1081 gcgatctcgt tcgctttttc tgttccgcag agcagttctg tcaatttcaa agcaaatgga
     1141 atcgccgctc ccatccctct tcctgtcgta acatgtccgt ctgtaaccgc cgcctcgtgc
     1201 acgacctgtg cacagtcaag tccttcttca aaagacggat atgcacatgc ttttcttcct
     1261 ttcaaaatac caagttcgct caaaatactc ggagctgcgc aaattgccgc aagatacttt
     1321 ttcttcttat cgaattcacc gatcagattc cgaaggcctt catgctcttt cagattcagc
     1381 gttcccggca ttccgcccgg aagtacgagc atatccccat ccgaaaaatc cgtctcctca
     1441 aacagccgat ccgcttccac aatgattttg tgtgccccgt taatctgttt ctgtccggta
     1501 atcgagatcg tctccacatc gatcttagcc ctgcgcataa tatcaacaac cgtcaatcct
     1561 tcaatttctt caaatccgtc cgccaaaaaa atgtatactt tcaaattact acctccgtat
     1621 tttacttttt ctttcttttc cgcattcgct ctgctataat cattatagct taaaatcagc
     1681 ggcgaattca actcaaagca ttgctccttg catagttttt acaggtaaaa ccggctcatc
     1741 gccgctgcaa taaaatttat gccgcaaaaa tgcggcggaa tggagaatat ttatggaaat
     1801 cgaacgaaaa tttcttgtag atctttccga tcttccgttt gatccgaaag attatccggc
     1861 cagaaagatc gagcaggctt atctatgtac tgccccggtc gtccgtatcc gaagagataa
     1921 tgacgatttt ttccttactt acaaatcaaa aggaatgatg gtaagagaag agtacaacct
     1981 tcctctcaca aaagaaagtt actatcacct cctgttaaaa gccgacggaa ggatcattac
     2041 gaaaacccgc tatgtcatcc ctatcgaaaa cggccttttt gttgaactgg acatttttga
     2101 aggcgacctt gcccctctcg ttctcgctga gatcgaattt cccgatgaag cgtttgccaa
     2161 agcttatcag gctccttcat ggctcaaaga agacgtaacc tactcttccg aatatcacaa
     2221 cagcaccctc agcaaaaaag attgaaacag gaaactcatc ggcttcagac aatgggcagt
     2281 tcacccttgt cttttgattt ttcaagttac tatcgttata ttatttttat tttcttcatg
     2341 gacatgcgtt ctctgtcgtg atactatatt acagtaaatc gtttctgcat ctgctctttt
     2401 tagttagatg tacgcatacg atcaccgtat ttacacggca gcttacagtt taatccggag
     2461 gaataagtat catgggactt tgcaacaaca gaattgtagt aaaagtggga acatccacat
     2521 tgacaaacga cgcgggaaaa agtgatcttc gcgccatcga ccgccttgtc tgcacacttg
     2581 ccgacatcca gaatatggga tatgaggtca tccttgtatc gtcgggagcg atcgcagtcg
     2641 gccgtaacaa gctgaatatg cacgaaaagc cggacagcat gcgtatgaaa caggctgccg
     2701 cagcagtcgg acaatgcagt ctgatgtatt tatatgataa atttttcgga gattatgata
     2761 aaacagtcgc tcagatcctt ctcaatgcag aagatatcga gcaggaagaa aagaaagaaa
     2821 acttaacgaa cacatttgac gccctgcttg aaatgggagt cattccgatc gtaaacgaaa
     2881 acgattccgt aagttataag gaaattgaat ccgaagacag acttttcggc gacaacgata
     2941 tgctttcttc tgtcgtatcc gttctctgcc gcgctaagcg acttgtcatt ttttctgata
     3001 tcgacggttt ttatgacagc gacccccgcc ttcacccgaa cgcgaaactg atcaaagaga
     3061 tccgtcagat cgatgaaagc gtctattctc tcgcaggcgg cgcaggatca agacgcggaa
     3121 caggcggtat gaggacaaaa cttcaggctg ccgaacttgc cgcttcacag ggcatcgata
     3181 cgatcgtgac gaacggaaaa caccctgaag ctttgtacga tatcgtaaac ggaaaacagg
     3241 cgggaacttt atttgtcgga aataggggtg aggaaggcat ttggtgctac tgccaatgtc
     3301 tt
//
LOCUS       DBMDXF010000198         3297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6544_length_3297_cov_2.357635, whole genome shotgun sequence.
ACCESSION   DBMDXF010000198 DBMDXF010000000
VERSION     DBMDXF010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6544_length_3297_cov_2.357635"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="ACZPD4_07510"
     CDS             <1..700
                     /locus_tag="ACZPD4_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR1378935.1"
                     /translation="KINGISYPTSGHGFARDNDFKCICTKPDKAAFLFSSGEETKEVY
                     PFDFELIVTYILKENSLIVKWEVHNTGDDDMYFTIGGHPAFRFQKADEKKTDYKLRFP
                     DKKALEYILVDLSECVADPGTVYKLELEDGFCALNEEMFEHDALIFDGGQFDEVWLCH
                     KGGEPYVGMKCKGFPNFGIWSVKDAPFVCLEPWMGRCDDVGFDKELSEKPYINKVKPG
                     ESFEQSYEILTAEC"
     gene            792..1568
                     /locus_tag="ACZPD4_07515"
     CDS             792..1568
                     /locus_tag="ACZPD4_07515"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1378936.1"
                     /translation="MLLTIDIGNTNITMGVFEEEFLLGMFRMTTKRARTSDEYGFTIC
                     GILEHRGIDSKQIDSVIVASVVPDIMHSFTSGIIKYLGTEPMIVSSQTDTGVRVVTEN
                     PTRTGPDRIVNAAAGYAIAGGPAIVVDFGTATTYDLIGPECTLEACVIAPGIETAGRS
                     LWDGAAMLPAIQIRRPESVLASETISGMQAGLVYGAIGQTEYIVKKMKEESGYKNAYV
                     IATGGLGKIIADETECIDLYDPQLTLNGLRMIHEKVQKKR"
     gene            1565..2524
                     /gene="dusB"
                     /locus_tag="ACZPD4_07520"
     CDS             1565..2524
                     /gene="dusB"
                     /locus_tag="ACZPD4_07520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1378937.1"
                     /translation="MKKLKIGNVELENRYLLGPMAGVTDLPFRLLCKEQGAGLLGMEM
                     VSAKAILYQNKNTEKLMEIHPEEQPVSLQLFGSDPKIMSEMAKRIEERPFAILDINMG
                     CPVPKVVKNGEGSALMKNPSLVYEIVNAIVKAIEKPVTVKIRKGFDDAHVNAVEVARA
                     AEEAGASAVAVHGRTREQYYSGEADWDIIRQVKEAVSIPVIGNGDVTTPEKAEELVQT
                     TGCDGIMIARGAQGNPWIFSEMIGKEKNGVTPPRPDKEAIRNMILRHSKLQIQYRGEF
                     AGMREMRKHVAWYTKGLTGAADLRRRVNETETLDELMMLLEEL"
     gene            2774..>3297
                     /locus_tag="ACZPD4_07525"
     CDS             2774..>3297
                     /locus_tag="ACZPD4_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR1378938.1"
                     /translation="MKNLKWKKVGSAVLATALAGSMVLPATAYAQGEIVQLEGGTSTQ
                     TNTAPEQVFLNKYSGTVRTQNFNDNWKFYLGDASGAQTPAFDDSSWDQVNLPHDYSID
                     QKYNRSMDGESGYLPGGTGWYRKSFTVDKSLEGKRISIDFGGVYMNATIYVNGKKLGT
                     HPYGYTPFSFDITD"
BASE COUNT         1056 a          592 c          883 g          766 t
ORIGIN      
        1 aaagattaac ggaatatctt atccgacgtc aggacacgga ttcgcaaggg ataacgattt
       61 taaatgcatc tgtacaaagc ccgataaggc agcgtttttg ttctcatcag gggaagaaac
      121 aaaagaagta tatccctttg attttgaact gatcgttaca tatattctca aagaaaacag
      181 cctgatcgtg aaatgggaag tgcacaatac cggagacgac gatatgtatt ttacgatcgg
      241 tgggcatccg gctttccgtt ttcaaaaagc ggatgagaaa aagacggatt ataagttgag
      301 atttccggat aaaaaggcac ttgaatatat tctggtagat ctctccgaat gtgtagccga
      361 tcccggcaca gtgtacaagc tggagctgga agatggattc tgcgctttga atgaggaaat
      421 gtttgaacac gacgcactga tttttgacgg cggtcagttt gacgaagtat ggctctgtca
      481 caaaggtgga gaaccatatg taggcatgaa atgtaaagga tttccgaatt tcggcatttg
      541 gtctgtgaaa gacgctcctt tcgtctgtct tgaaccgtgg atgggacggt gcgacgatgt
      601 cggctttgac aaagaacttt cggaaaaacc atatatcaat aaagtgaagc cgggagagag
      661 ctttgaacaa agttatgaaa tattgacggc agaatgttga tcggccgatc agataaaatc
      721 gaaaatatat attgaatata tttttggaaa ataccgatga aaagattcgg tacagcggga
      781 ggaagaagac aatgcttctt acgattgata taggaaatac aaatattaca atgggtgtgt
      841 tcgaggaaga gttcctgctc ggtatgttcc gtatgacgac aaaacgggcg aggacatcag
      901 acgaatacgg attcaccata tgcggaatac tggagcacag aggaatcgac tcaaagcaga
      961 tcgattcggt gatcgttgcg tcggttgtcc ccgacattat gcactctttt acaagcggta
     1021 ttatcaaata tttaggaacg gaaccgatga tcgtatcttc tcagacagat acaggagttc
     1081 gtgttgtgac tgaaaatccg acgcggacag gaccggacag aatcgtcaat gcggcagcgg
     1141 ggtatgctat tgcaggagga cctgcgatcg tggttgattt cggaacggct acgacgtatg
     1201 atttgatagg tcctgaatgt acgcttgaag catgtgtgat cgccccgggc attgaaacgg
     1261 caggacgttc tctgtgggac ggagcggcga tgcttccggc gattcagatc cgaagaccgg
     1321 aatccgtgct tgcatcggag acgattagcg gaatgcaggc aggacttgta tacggagcga
     1381 tcggacagac ggaatatata gtaaaaaaaa tgaaagaaga gtcgggatat aaaaatgcct
     1441 atgtgattgc cacaggcggg ctgggtaaaa ttatcgccga tgaaacagaa tgcatagatc
     1501 tgtatgatcc acagcttaca ctgaacggct tgcgaatgat tcatgagaaa gtgcagaaaa
     1561 aaagatgaag aagctgaaaa taggaaatgt agaattggaa aaccgatacc tattaggacc
     1621 tatggcaggg gtgacagacc tgccttttcg tttgctgtgc aaggaacagg gagcgggtct
     1681 tttagggatg gaaatggtta gtgcaaaagc aattttgtat caaaataaaa atacagagaa
     1741 actcatggaa atacatccag aagagcagcc ggtatctttg cagttgtttg gttctgatcc
     1801 gaagattatg agcgagatgg cgaaaagaat tgaagaacgt ccttttgcga ttctcgatat
     1861 caatatgggg tgtccggtac cgaaagttgt aaaaaacggg gaggggtctg ctttgatgaa
     1921 aaacccctct cttgtttatg agattgtaaa cgcgatcgtt aaagcaatag agaagcccgt
     1981 tacggtaaaa atccgaaaag ggttcgacga tgcgcatgta aatgcagtcg aagtcgcaag
     2041 agcggcggaa gaagcgggag cgtcggcggt tgcggttcac ggaaggacga gagagcagta
     2101 ttatagcgga gaggcggact gggacattat caggcaggtg aaagaggcgg tttccattcc
     2161 tgtaatcgga aacggcgatg tcactacacc ggaaaaagca gaagaacttg ttcaaacgac
     2221 aggatgcgac ggaattatga tcgcaagagg cgcacaggga aacccgtgga tcttttcgga
     2281 aatgatcggc aaggaaaaaa acggagtaac tccgcccaga cctgataaag aggcaatacg
     2341 gaatatgatc ctcaggcatt caaagcttca gattcagtat cgcggagaat ttgcgggaat
     2401 gcgggagatg cgcaaacatg tagcctggta cacgaaagga ctgacaggag cggcagatct
     2461 ccggcgcaga gtaaatgaga cagagacatt agatgaactg atgatgttat tggaagaact
     2521 atgaaaatct gcacaaaaaa tatcctttaa tatgtgaagc ggcaagataa aaatagtaaa
     2581 aatgccaaaa agcaaaaacg accttacaaa aatgttacgg taatctatac aaaataaaca
     2641 aagttttgta taatttatat attacgcata gtaacatgga gaaaaaggtc gtttttcttt
     2701 atttcgtttc atggaatatg cgtgaaaatt cagaacgaag gagtgctctg gaactatagg
     2761 ggcgaaaaag tttatgaaaa atctcaagtg gaagaaagta ggttcggcag ttcttgctac
     2821 agctttggca ggaagcatgg tgttgccggc aacagcctac gcacagggtg agatcgttca
     2881 actggaaggg ggaacttcga cgcagacaaa cacagcgccg gagcaggtgt tccttaacaa
     2941 atacagcgga acggtcagaa cgcagaactt caacgataac tggaagttct atttaggaga
     3001 cgcaagcgga gcgcagactc cggcatttga cgactcctca tgggatcagg tgaacctgcc
     3061 gcatgattac agtatcgacc agaaatataa ccgttcaatg gatggagaga gtggatatct
     3121 tccgggcggt acaggatggt acaggaaaag ctttactgta gataagagcc ttgagggcaa
     3181 acgtatcagt atcgacttcg gcggcgtgta catgaatgca actatttatg taaacggtaa
     3241 aaagctcggt acacatccgt atggatacac accattctct ttcgatatta cagataa
//
LOCUS       DBMDXF010000199         3285 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6585_length_3285_cov_1.420854, whole genome shotgun sequence.
ACCESSION   DBMDXF010000199 DBMDXF010000000
VERSION     DBMDXF010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_3285_cov_1.420854"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="ACZPD4_07530"
     CDS             complement(<1..659)
                     /locus_tag="ACZPD4_07530"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR1378939.1"
                     /translation="MKNILFAASECVPFIKTGGLADVVGSLPKDFDKEKYDVRVVIPN
                     YMCMKQEWKEKLEYVAHFYMDIAGQDRYVGVLKIELNGIIFYFIDNEYYFSGFAPYDG
                     DPKWNIEKFAFFSKAVLSILPVIGFRPELIHCHDWQTGLIPVYLDNFRYLGEYYRGIK
                     TVMTIHNLKFQGVWDTKSVREITGLPEYYFVPDKMEAYKDANLLKGGIVYADKVTTVS
                     N"
     gene            complement(752..1543)
                     /gene="spo0A"
                     /locus_tag="ACZPD4_07535"
     CDS             complement(752..1543)
                     /gene="spo0A"
                     /locus_tag="ACZPD4_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HIR1378940.1"
                     /translation="MEHLSVAIADDNQRILDMLDDIIQTDKELDLVGKAKNGEEMCQI
                     IKDRQPDVVLLDLIMPKMDGLTVMEQVNKQEFVDKRPYFIVLTAVGQEKITEDAFNKG
                     ANYYIMKPFNHTILLDRIKSIRKKPAVFEKKNEESIQEVSSRRENLESRVTNMLHEIG
                     IPAHIKGYHYLRDAIMMAVNDMDVLNAITKILYPTVAKKYRTTSSRVERAIRHAIEVA
                     WSRGKLDTLDELFGYTVSTGKGKPTNSEFIALIADTIQLEYRHKN"
     gene            complement(1651..2742)
                     /gene="spoIVB"
                     /locus_tag="ACZPD4_07540"
     CDS             complement(1651..2742)
                     /gene="spoIVB"
                     /locus_tag="ACZPD4_07540"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HIR1378941.1"
                     /translation="MLKKQKYVTAVFCLFLSFFFAAMGILQYQMIRGKKPVSTDAGIE
                     AMNNDTVLVGGMPIGIYMETDGVMVLNTEQIAGADGKEHEPAKGIVKAGDYIMAVDHC
                     EITGKKELLEAVGNLTGTFVVLTVRRNGETIDLKIKPVEYETGEYRLGIWVRDNVQGL
                     GTVTFLTDQSRFGALGHGIHDVDTSVLMSIAEGNVYRTSIRDITKGQSGSPGSMEGMI
                     IYNNYNILGTIDKNTEAGIYGKIDKIDELFTEQTAIRTAKKEEIITGPATIRCFVDNE
                     VREFDIEVTQVDMMSQEINKGLVIKVTDEELLEKTGGIIQGMSGSPIIQNGKLIGAVT
                     HVFVQDSTKGYGIFIENMLKQVNLPEMCG"
     gene            complement(2875..>3285)
                     /locus_tag="ACZPD4_07545"
     CDS             complement(2875..>3285)
                     /locus_tag="ACZPD4_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1378942.1"
                     /translation="PEKPLENIASGGELSRIMLAIKTVLADAEDTPTLIFDEIDAGIS
                     GVTAAKVGEKLKLIGKSRQVICITHLSQIAAVADSHFLIEKSAENESVKTRIRLLNEE
                     HSVKELARILGGDKITSSIMESAREMKEMAKSEI"
BASE COUNT          842 a          846 c          493 g         1104 t
ORIGIN      
        1 ctgttactta ccgttgtcac tttatcggca tatacgatac cgcctttaag caagtttgca
       61 tctttatatg cctccatctt atccggaacg aaatagtatt ccggaagtcc ggtaatttcg
      121 cgaacagatt tcgtatccca taccccctgg aatttcagat tgtggattgt catcacagtt
      181 ttaattccgc gatagtattc tcccagatat ctgaaattat caagataaac cggtatcagt
      241 cccgtctgcc agtcatgaca atgaatcagt tccggacgaa atccgatcac cggaagtatg
      301 ctcagcactg cttttgaaaa gaaggcaaac ttttctatat tccacttcgg atccccatca
      361 tacggagcaa atccactgaa ataatattca ttatcgataa aatagaaaat aataccgttc
      421 aattcaatct tcagtactcc gacatacctg tcctgacctg caatgtccat ataaaaatgg
      481 gcaacatact ctaacttctc tttccactcc tgtttcatgc acatgtaatt cggaataaca
      541 acacgaacat catatttttc tttgtcgaaa tctttcggaa gcgaaccgac aacatctgcc
      601 agacctccgg ttttgataaa cggtacacac tccgatgcag caaacaaaat atttttcata
      661 tcctgatcct cccattcttc acataaattc ccggaaagaa ttctgttaat tgttattata
      721 ccctattctc tgcgcaatga aaaggacttt cctagttttt atgcctgtat tcaagctgaa
      781 ttgtatcggc aatcagcgca ataaactctg aattggtcgg tttccccttt cctgtactca
      841 cagtatatcc aaaaagctca tcaagcgtat ccaattttcc gcggctccat gccacctcga
      901 tcgcatgtcg aattgcccgt tcgacgcggc ttgacgttgt ccggtatttt tttgcaactg
      961 taggatacaa tatttttgta attgcattca acacatccat gtcgtttact gccatcatga
     1021 tcgcatcccg cagataatga taccctttga tatgcgccgg tattccaatc tcatgaagca
     1081 tattcgtcac tctgctttcc agattttccc gtcttgatga aacttcctgt atactctctt
     1141 catttttctt ttcaaaaacc gccggctttt ttcggatcga tttgatccga tccaataaaa
     1201 tcgtatgatt aaacggtttc ataatatagt aatttgcacc tttattaaat gcatcttctg
     1261 taattttttc ctgtccgaca gcagtaagca ctataaaata cggtcttttg tcgacaaact
     1321 cctgcttgtt tacttgctcc attactgtca atccatccat tttcggcatg atcagatcaa
     1381 gcaggacaac atccggctgt ctgtccttta tgatctgaca catctcttct ccatttttcg
     1441 cttttccgac gagatccagt tccttgtccg tttgtatgat gtcgtcaagc atatctagaa
     1501 ttctctggtt gtcgtcagcg atcgccacac tcaaatgctc cattctcgtc tctcctttag
     1561 ttttgagacc atccctttaa aacgccactc gcttattata tctatgccat tttgcataat
     1621 caagcgaatc ttgtagtaac aattcgacat ttatccacac atttccggta aattcacctg
     1681 ttttaacata ttttctatga atattccgta accttttgtc gagtcttgta cgaaaacgtg
     1741 ggttactgct ccgatcaatt tcccgttttg tatgatcgga cttccgctca tcccctgaat
     1801 aatccctcct gttttttcaa gaagttcttc gtccgtcacc ttaatcacaa gtcctttatt
     1861 gatctcctga gacatcatgt cgacttgagt cacttcaata tcaaattctc taacttcatt
     1921 atctacaaaa catcggattg tagcaggtcc tgttataatt tcttcttttt ttgcagtcct
     1981 gattgccgtc tgttctgtga aaagttcatc aattttgtcg attttcccat aaatcccagc
     2041 ctctgtattt ttatcgattg taccgagaat attataattg ttataaatga tcataccctc
     2101 catacttccc ggagatccgc tctgtccttt tgtgatatct ctgatactcg tgcggtatac
     2161 atttccttcc gcaatcgaca taagaacact tgtatccacg tcatgaatcc catgcccaag
     2221 cgcgccaaac cgactttgat ccgttaaaaa agtaactgtt cccaagcctt gtacattatc
     2281 gcgaacccat ataccaagcc gatattctcc cgtttcatat tcaaccggct ttattttcaa
     2341 atctatcgtc tccccatttc gtctgaccgt caacaccacg aacgttccgg ttagatttcc
     2401 gacagcctcc aaaagctctt tttttcccgt aatttcacaa tgatctactg ccataatata
     2461 atcgcctgct ttaacaattc cttttgctgg ttcatgctcc tttccatccg ctccggcaat
     2521 ctgctctgta ttaagaacca tcactccgtc cgtttccata taaataccaa tcggcattcc
     2581 tccgacaagc accgtatcat tattcatcgc ttctattccc gcatctgtcg aaaccggttt
     2641 ctttccacga atcatttgat actgcaaaat acccatagcc gcaaagaaga aggagaggaa
     2701 tagacagaat acagcggtca catacttttg ttttttcaac atatccacac cctttctctg
     2761 atatctcgat aattttttat ccgcatccgc tccttctttc ctatataaaa agaaacggat
     2821 acttctctgt atccgtttct agtatatgtg gtttcttctt tttccacact agtattatat
     2881 ttcactcttt gccatttctt tcatttcacg ggcactttcc ataatactgg atgtaatctt
     2941 atctccaccc agaatacgcg ccagttcttt taccgaatgt tcttcgttca atagacgaat
     3001 ccttgttttt acgctttcgt tttccgcaga tttttcaatc agaaaatgac tgtctgccac
     3061 tgctgctatc tgtgaaagat gagtaatgca aatcacctgc ctgcttttac ctatcagctt
     3121 tagtttttct ccgacttttg ctgccgtaac tcccgatatt ccggcatcaa tctcgtcaaa
     3181 aatcaaagtc ggcgtatctt ccgcatctgc cagaaccgtt tttatcgcaa gcataatacg
     3241 ggaaagttct ccgcctgatg caatattctc aagcggcttt tcagg
//
LOCUS       DBMDXF010000200         3276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6599_length_3276_cov_2.453858, whole genome shotgun sequence.
ACCESSION   DBMDXF010000200 DBMDXF010000000
VERSION     DBMDXF010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_3276_cov_2.453858"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..56)
                     /locus_tag="ACZPD4_07550"
     CDS             complement(<1..56)
                     /locus_tag="ACZPD4_07550"
                     /inference="COORDINATES: protein motif:HMM:NF037321.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378943.1"
                     /translation="MSLTYHKGINQLTCHYCG"
     gene            complement(132..857)
                     /gene="spoIIR"
                     /locus_tag="ACZPD4_07555"
     CDS             complement(132..857)
                     /gene="spoIIR"
                     /locus_tag="ACZPD4_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR1378944.1"
                     /translation="MDRTKIKDQFGQFGRRREYGADDDRTTSRQMKKSRRMCAAAAVL
                     FAFAAAFALTTAVYRMDAKAQSDLQRRLSEEVLRFHVLANSDSREDQALKLKVRDKVL
                     EFLEANMADEQHMTEDETVMWIREHIDEIEDVSRRCVAEENYDYPVTAAVTTCWFPDK
                     TYGDVTFPAGNYEALRIEIGSAKGHNWWCVLYPSLCFRDAANAVVPNEGKEKLKHVLT
                     EEEYSEITAGTEFKISWYFWKKK"
     gene            complement(930..1613)
                     /locus_tag="ACZPD4_07560"
     CDS             complement(930..1613)
                     /locus_tag="ACZPD4_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1378945.1"
                     /translation="MPIDFEVTMNEYLPLRDVVFNTLRRAILRGELKPGERLMEIQLA
                     NKLGVSRTPIREAIRKLELEGLVLMIPRKGAEVAEITEKNLRDVLEVRCALEELAVQL
                     ACDRINQEEIHELHAAAARFEQMFDSDDITSIAQADEAFHDVIFQATDNERLIQLLNN
                     LREQMYRYRIEYLKKKECYPQLIAEHEAIMQAIEGHDKAMATQITGQHINNQVDTVVG
                     TLRSKMPDF"
     gene            complement(1615..2490)
                     /gene="ispE"
                     /locus_tag="ACZPD4_07565"
     CDS             complement(1615..2490)
                     /gene="ispE"
                     /locus_tag="ACZPD4_07565"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1378946.1"
                     /translation="MNEIELKSLAKINLGLDVLGRRENGYHDVRMVMQSIYLHDEVKI
                     EKKRREGIEIVSNLRFLPIGEGNIAYKAAHMLIEEFSLEGGVRITLNKHIPVAAGLAG
                     GSSNAAAVLFGMNRLFRLGLSKEELMERGVKLGADVPYCIMRGTVLAEGIGEELKELP
                     PMPKCTVLIAKPPISVSTKVVYEALDAKEISEHPDIDGVIEGLEEGSLKKVASAMGNV
                     LEDVTIPMHPVIEEIKQEMKECGALNAMMSGSGPTVFGLFENRTAAREAQRRIREKSL
                     AGQVYVTSIHGVRRN"
     gene            complement(2560..>3276)
                     /locus_tag="ACZPD4_07570"
     CDS             complement(2560..>3276)
                     /locus_tag="ACZPD4_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR1378947.1"
                     /translation="SMKMNVYSEVETEILKDMYSLDRECVLEKKEGIYEEMLMYNQAK
                     CKIAERLTLPELKEDVLQIIHSEGSIQAESERYTEEGVEIEGILHISFLYLRADDEQP
                     YGSWTGMIPFSYLVEYPNLPKNVSASPAYHVEQLAVTLAGSEAVEVKAVLTFDIFLKK
                     LISASMITNVSERQFDTDASVKRPGIVGHIVQDGEDLWSLAKKYMTTVSGIMEVNSMD
                     NENVKTGDKLLIFKENMSIL"
BASE COUNT          756 a          931 c          558 g         1031 t
ORIGIN      
        1 tatccgcaat agtggcaggt cagttggttg atacctttat gataagtcag actcacatcg
       61 ccgttcttac agatatcttc tctacgtcct cataatcttt tacgaaatgt tctattaaaa
      121 attgtgtcat tttatttctt cttccaaaaa taccagctta ttttaaactc tgttcctgcc
      181 gtgatttctg aatactcctc ttctgtcaaa acatgtttca atttttcttt tccttcattc
      241 ggaacgactg catttgccgc atcgcgaaaa cagagactcg gatagagaac gcaccaccag
      301 ttatgtcctt ttgccgaacc gatctcaata cgcagcgcct cataatttcc tgccggaaac
      361 gttacatcgc catatgtttt atcgggaaac cagcaagttg tcaccgccgc cgttacagga
      421 taatcatagt tttcttcggc aacacaccgg cggctcacat cctcgatctc atcgatatgc
      481 tcccgtatcc acatcacggt ttcatcctct gtcatatgct gttcatccgc catgtttgcc
      541 tccagaaact ccagcacttt atcacgcact tttaatttca gcgcctgatc ttccctgctg
      601 tcgctgtttg ccagaacatg aaagcgaagc acttcttctg ataatcgtcg ctgaagatca
      661 ctctgcgctt tcgcatccat cctgtaaacc gccgttgtca gagcaaacgc cgcagcgaat
      721 gcaaatagta ccgcagctgc ggcgcacatc cgccttgatt ttttcatctg ccggctagtc
      781 gttctgtcat catccgcccc gtattcccgc ctcctgccga actgtccgaa ttgatctttg
      841 atctttgttc tgtccatatg tattccctcc gtctgctgtt tttataacta tgctacagag
      901 agtatggaca tttctttttc ttttatacat caaaaatccg gcattttact gcgcagcgtt
      961 ccgactacgg tatctacctg attattgata tgctgcccgg taatctgagt cgccattgct
     1021 ttgtcgtgtc cttcgattgc ctgcatgatc gcttcgtgtt ctgcgataag ctgcggatag
     1081 cattcctttt tcttcaaata ttcaatccgg tagcggtaca tctgttcgcg tagattgttc
     1141 aaaagctgaa tcagtctttc attatccgtc gcctgaaaga tcacatcatg aaacgcctcg
     1201 tctgcctgtg cgatacttgt tatatcgtca ctgtcaaaca tttgttcaaa tctggcagca
     1261 gcggcatgaa gttcgtgtat ttcctcctga ttaatgcggt cacacgcaag ctgtacggca
     1321 agttcttcaa gcgcacaccg aacttccagt acgtcgcgca gatttttttc tgtaatctcc
     1381 gctacctccg cgccttttct cggtatcatg agcacaagtc cttccaattc cagctttcgg
     1441 atcgcttccc ggatcggcgt tctgctcact cccagtttgt ttgcaagctg tatctccatc
     1501 agccgctctc cgggctttag ttctcctctt aagattgccc gcctgagcgt attgaatacg
     1561 acatctctca agggaaggta ttcattcatc gtcacttcaa aatcaatcgg catcttaatt
     1621 cctcctcact ccatggatac tggtcacata cacttgtcct gccagtgact tctccctgat
     1681 acgtctctgt gcttcacgcg ccgcagtcct gttctcaaac aaaccgaaca cagtcggacc
     1741 gcttccgctc atcattgcgt ttaaagctcc gcactctttc atttcctgct tgatctcttc
     1801 aataaccgga tgcataggaa tcgtcacatc ttccaaaaca tttcccatag cagaggcaac
     1861 ctttttcaga ctgccttctt ccaatccttc gatcaccccg tcgatatcag gatgttctga
     1921 gatttctttc gcatccagag cctcatatac aacctttgta gaaacactga tcggcggttt
     1981 tgctataaga accgtacact tcggcatcgg aggaagttct tttagttctt ctccgatccc
     2041 ttctgccaga accgttcctc tcataatgca gtatggtacg tctgcgccga gcttcactcc
     2101 tctttccatc agttcttctt tagataaacc gagccggaac aaccggttca taccgaacaa
     2161 aaccgccgcc gcattcgagc ttcctcccgc aagtcctgcc gcaaccggga tatgcttgtt
     2221 caaagtgatc ctgactccgc cttccaatga aaattcttct atcagcatgt gcgccgcttt
     2281 gtatgctata tttccttcac ctataggaag gaatcttaaa ttcgatacaa tctcgattcc
     2341 ttcccttctc ttcttctcga tcttcacttc atcatggaga taaatcgact gcattaccat
     2401 ccttacatca tgataaccgt tttctctcct ccccagcaca tccaggccaa gattgatctt
     2461 tgccagtgat ttcaattcaa tctcattcat cttccgtcct ccgcccgttt tccgcctctg
     2521 tgtatctgta catttacttg tatcttgtat acattatgct tacagtatac tcatattttc
     2581 tttaaaaatc aataatttat cccccgtttt cacattttca ttgtccatag aattaacttc
     2641 cataataccg gataccgtcg tcatatattt ttttgccaga ctccacaaat cttccccatc
     2701 ctgcacaata tgaccgacga tccccgggcg ttttacagaa gcgtccgtat cgaactgccg
     2761 ttcggaaaca ttcgtgatca tacttgccga tatcagcttt ttcaaaaaga tatcaaaggt
     2821 cagcaccgcc ttcacttcga ccgcctcgct tcccgcaaga gtcacggcaa gctgctcgac
     2881 atgatacgcc ggacttgcgc tgacattttt cggaagattc ggatattcca caagatacga
     2941 aaacgggatc atccctgtcc aacttccata tggctgttcg tcatccgctc tcagatagag
     3001 aaaagaaata tggagtattc cttcaatctc cactccttcc tcagtgtacc gctcgctttc
     3061 tgcctggata ctgccttcac tgtgaataat ctgcaaaacg tcctctttca gctcaggaag
     3121 cgtcagacgc tccgcaatct tacattttgc ctgattgtac ataagcattt cctcataaat
     3181 gccttctttt ttctcaagta cgcactctct gtcaagagaa tacatatctt ttaatatctc
     3241 tgtttccact tcagaataaa cattcatttt cataga
//
LOCUS       DBMDXF010000201         3276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6601_length_3276_cov_1.603780, whole genome shotgun sequence.
ACCESSION   DBMDXF010000201 DBMDXF010000000
VERSION     DBMDXF010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_3276_cov_1.603780"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="ACZPD4_07575"
     CDS             complement(<1..334)
                     /locus_tag="ACZPD4_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1378948.1"
                     /translation="MAVTVKQIAELANVSRGTVDRVLNNRSGVSEATRQKVLKIAKEL
                     HYEPNFLAKALVSKKESLKIGVILTPDYNPFIHDIISGINRAKKEFSAFGIEVIIKLM
                     TSLDPSEEI"
     gene            589..1455
                     /locus_tag="ACZPD4_07580"
     CDS             589..1455
                     /locus_tag="ACZPD4_07580"
                     /EC_number="5.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-psicose 3-epimerase"
                     /protein_id="HIR1378949.1"
                     /translation="MKFGTLYSYWGTKWQCDYLKTLKRVSDIGFDILEMGAPHLLEMS
                     DYELSELRRAAKDMDMVLTANIGPAKDKDLASPDPDIRKAGVNYLIDILKAMEKVGSK
                     SLVGAMYSYWPCQFEITDKEAAWERSIEGMKEVAEAAESLGIECCQEVLNRYETYIIT
                     DCREGLEYCRRVGSENVNLLLDTFHMNIEEDNIPEAIRLAGRKLGHLHVGESNRKLPG
                     MGSLPWRDIGRALRDIGYEKGVVMEPFLLQGGEVARDCKVWRDLSGNADEKMLDRYIK
                     ESLTFLKHEFTF"
     gene            1470..2405
                     /locus_tag="ACZPD4_07585"
     CDS             1470..2405
                     /locus_tag="ACZPD4_07585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR1378950.1"
                     /translation="MAEIWTMGEMIVEIMREKENEPLDKAGVFRGPYPSGAPAIFIDT
                     VARLGHKGGIIGGVGKDDFGKCLLDRLKSDGVDVSHVIENPKKATGCAIVTYFDDGSR
                     KFIFHMGNTPAVEAKAPQKDVLDGVRYFHVMGCSLFADIEFAKEILKAAQTAKKIGAK
                     VSFDPNIRKELIGEKETDKILENLMRLTNVFLPGEEELLLLTGKETVEEAVEECFKNP
                     ELEILALKQGSRGSKIYTKEKTVETGIYRIQPVDATGAGDSFDAAFICGLCEGKRIEE
                     AAKMAAAAGAINTAAFGPMEGKISVEAIDKMIKEE"
     gene            2408..>3276
                     /locus_tag="ACZPD4_07590"
     CDS             2408..>3276
                     /locus_tag="ACZPD4_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1378951.1"
                     /translation="MKKVNIGVAGLGFGKEFAAIYCDHPLVDKVAICTRNPETLNKVG
                     EELGIPEELRFTNYDDMISHEELDAIHIVTPIKEHYPQSIKALRAGKHTACTVPMATS
                     LDELREIVKTSREVGKIYTMMETSLYTREYLYVKRLKENGELGKIQYLKGDHMQNMSL
                     EGWGDYWQGFPPFWYGTHVLSPILDLAGTTAKSVRCLGSGRVNKERAEHYGCPYAVET
                     ATFRLRNSDIVAEAHRCLFETVRQVRECFDVYGDKMSFEWEPTVDEGHTIFTGIDDFT
                     KFNRFDTNAAAKGG"
BASE COUNT         1086 a          482 c          853 g          855 t
ORIGIN      
        1 ggatttcttc ggaaggatca agactggtca tcagtttgat gattacttcg attccaaaag
       61 cagaaaattc cttcttggca cggttgattc ccgaaataat atcatggata aaggggttat
      121 aatccggtgt taagatgacc ccgattttta aggattcctt tttagatact aacgctttgg
      181 caaggaagtt cggttcatag tgcagttctt ttgcgatttt caaaactttt tggcgcgttg
      241 cctcgctgac tccggaacga ttgtttagta cccggtctac agtacctcga gaaacgtttg
      301 ccagctctgc aatttgtttt acggttaccg ccatgtaata atcacctccc atataatacc
      361 atttttactg ctaataatat aacataatat cgtgctcgtg tctagttaca aaaaataaga
      421 gtttgtgcaa aaaaaatata tgtaacatac aactcaatat aaatgtaaaa aatgcacaaa
      481 aaatacagtg aaaaaatggc gaatatatag tattgaaaca ataaaatgaa catggtacta
      541 taaatacaag aaaaacgtgc acgagcacga aaaagaagga ggatgaagat gaaatttgga
      601 acattatatt cttattgggg aacaaaatgg caatgtgatt atttgaaaac attaaaacga
      661 gtttcagaca tcggatttga cattttggaa atgggtgctc ctcacttgtt ggaaatgtca
      721 gattatgaac tttcagaatt gaggcgcgcg gcgaaagata tggatatggt attgacggca
      781 aatatcggac cggcaaaaga taaagatctt gcttctcctg atccggatat acgaaaagcg
      841 ggagtaaact atttgatcga tatattaaaa gcaatggaaa aagtaggatc taaatcactt
      901 gttggagcaa tgtattctta ttggccgtgt caatttgaaa taacggataa ggaagctgcc
      961 tgggagagaa gcatcgaggg gatgaaagag gttgcagaag ctgcggaatc attgggaatc
     1021 gaatgctgtc aggaagtttt gaatcgatat gaaacttata ttatcacaga ttgcagggaa
     1081 ggattggaat actgcaggag agtcggaagt gaaaacgtta atctccttct tgatacattt
     1141 catatgaata tcgaagaaga taatattccg gaggctatcc ggcttgcagg aagaaaattg
     1201 ggccatctgc atgtgggaga atcaaacaga aagcttccgg gaatgggatc ccttccttgg
     1261 agagatatcg gacgggcgct aagagatatc ggatatgaga aaggcgtcgt tatggaaccg
     1321 tttcttcttc aaggaggaga ggtcgctcgg gactgtaaag tgtggagaga tttaagtggg
     1381 aatgcagatg agaaaatgct ggatcgctat ataaaagaat ctttaacatt tttgaaacat
     1441 gaatttacgt tttgaggaga ggatatgaaa tggcagaaat ttggacgatg ggtgaaatga
     1501 tcgtggaaat tatgcgggag aaagaaaatg agccactgga taaagcagga gtttttcgag
     1561 gaccttatcc gagcggagca ccggcgattt ttattgatac agtggcgaga ctcggacata
     1621 agggcggaat tatcggagga gtaggcaaag acgattttgg aaaatgtctg ttggacagat
     1681 tgaaaagtga cggagtcgat gtgtcgcatg tgattgaaaa tccaaaaaaa gctacaggat
     1741 gtgcgattgt aacatatttt gatgatggct cccgaaaatt tatttttcat atggggaata
     1801 ctccggctgt tgaagcgaaa gcgccgcaga aggatgtgct ggatggagtt cggtattttc
     1861 atgtaatggg ctgttctttg tttgctgata tagagtttgc aaaagaaatt ttaaaagcgg
     1921 cacaaacggc aaaaaaaata ggggcgaagg tttcttttga tccgaatatc agaaaagaat
     1981 taatagggga aaaagagacg gataaaattc tggagaatct gatgcggttg acgaatgttt
     2041 ttttgcccgg ggaggaagaa cttcttttgt tgacaggaaa agaaacagtg gaagaagctg
     2101 ttgaagaatg ctttaaaaat cctgagcttg aaatattggc attaaagcaa ggttcgcggg
     2161 ggtcaaagat ttatacgaag gaaaaaacgg ttgaaacagg aatttacaga attcagcctg
     2221 tagatgcaac cggagccgga gatagttttg acgcggcgtt tatctgcgga ctgtgtgaag
     2281 gaaagagaat tgaagaggcg gcgaaaatgg cagcagcagc aggtgcgatt aatacagcgg
     2341 cgtttggtcc tatggaagga aagatcagcg tggaagcgat agataagatg ataaaggagg
     2401 aatgaaaatg aaaaaagtaa atatcggagt agcgggactt ggatttggaa aagaatttgc
     2461 ggcgatttac tgtgatcatc cgcttgttga taaagtggcg atctgtacaa ggaatcccga
     2521 aactttaaat aaagtggggg aagaactcgg aattccggaa gaattaagat ttacaaatta
     2581 tgatgatatg atttcacatg aagaattgga tgcgattcat attgtgacgc cgattaaaga
     2641 acactatcct cagtcgatca aagcgctaag agccgggaaa catactgcat gtacagttcc
     2701 gatggcgaca tctttggatg agttgagaga gattgtaaaa acatccaggg aagttggaaa
     2761 aatctatacg atgatggaaa cttcgcttta tacaagagaa tacctttatg taaagagatt
     2821 aaaagaaaat ggtgaacttg gaaaaattca gtatttaaaa ggtgatcata tgcagaacat
     2881 gtctctggaa ggctggggag actactggca gggatttccg ccgttttggt atggcacgca
     2941 tgtactgtca ccgattctgg atctggccgg tacaacagct aagagtgtgc ggtgtcttgg
     3001 aagcggaagg gtaaataaag aaagagctga acattacggg tgtccgtatg cggtagaaac
     3061 cgctacattt agattgagaa acagcgatat cgtagcagag gcacatagat gtcttttcga
     3121 gacagtacgt caggtaagag agtgttttga tgtttacgga gataaaatgt cgtttgagtg
     3181 ggagccgacg gtagacgaag gacatactat tttcacaggc attgatgatt ttacaaagtt
     3241 taacagattt gacacaaacg ccgcggcaaa gggtgg
//
LOCUS       DBMDXF010000202         3229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6715_length_3229_cov_1.605179, whole genome shotgun sequence.
ACCESSION   DBMDXF010000202 DBMDXF010000000
VERSION     DBMDXF010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_3229_cov_1.605179"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            101..1090
                     /locus_tag="ACZPD4_07595"
     CDS             101..1090
                     /locus_tag="ACZPD4_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1378952.1"
                     /translation="MKKRVIEFILLTVFAVLFLSGCKKNVGTPEDNPVVEEDEESVEP
                     AKDYLYGYSCADLKDPFYMALKDAIGVQAEQNKGQFIVRDAGNDPVKQNEQLMEMIDK
                     GADVVFLTASDPDAVTPALEALDKADIPVILLETQVKERDLAKAFISSDNYNAGKVCG
                     EDLKEKMPDGGKLVIVEDKADQNLSEGISGFEEKIKNGGFEVVKRVDGAQTDGMTKEL
                     NEILSSGTKVEVIMCADDQMAEAVRATVKESGKNDILIYSIGGSPAAKAALLAQEGAM
                     AGIGARSPIIEGKIAVKTADAILDDGSYEEEVLIETFFINKENVEIYGTDGWQ"
     gene            1106..2944
                     /locus_tag="ACZPD4_07600"
     CDS             1106..2944
                     /locus_tag="ACZPD4_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1378953.1"
                     /translation="MRKTEGSPLPFGAAAKERKVNFSVQVPMGKTCELLLYREGNTKP
                     EVCFDMPEEEGIGEVRFLSVEEIDPEKYEYNFLIDGKVCTDPYAKEIAGREVFGKRQN
                     DDKHGVRAKIQVSDYDWGEDRPIRHPWNEVVSYSIHVRGFTKHSSSKVLHRGTYQGVI
                     EKIPYLKELGVNQIQCMPIYEFDECAKTKINYWGYGPAFYFAPKEAYSSDGCAVRELK
                     DMVKACHKADIEVVLEMPFAAGISEQEAAECLRFYMMEYHVDGFVVNPYIVSWELLNK
                     DPFLKDIRLMRKDDGFQNVMRRFLKGDENMVNDVISSLGHNSASDGKCNYITAQTGFT
                     LRDLVSYDCKHNEENGENNTDGPDYNYSWNCGAEGQSRKRSVVNLRKSQIRNAFLLLL
                     LAQGTPCLLSGDEFYNTQKGNNNVYCQDNATGWLDWSRLKSDRGLFDYVKALITFRKN
                     HPCLCQKEPLKGMDRTACGMPDVSYHGENAWQVKNEVSSRQLGVMYSGAGVGDCDCFV
                     AYNMHWLPHSYALPSPGKGKCWHLALDTDRGVLSKSIKLEDQKRIEIRERSIAVLISC
                     EEDKTELRNTNFHSKGKTAEKKSVSETEKTDIVTSDAEKEKKCRQE"
     gene            2950..>3229
                     /locus_tag="ACZPD4_07605"
     CDS             2950..>3229
                     /locus_tag="ACZPD4_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1378954.1"
                     /translation="MKAIKHFQTITKHKILVMQECFRVGLYRQGLLHDLSKYGWTEFR
                     VGCRYYQGNRSPNNAEREDTGLSTSWIHHYGRNKHHFEHWVDYGVDCDT"
BASE COUNT         1051 a          500 c          872 g          806 t
ORIGIN      
        1 aagagaaaaa gcaagaagca acgtgattgt attgacattc atttgaaaca aggtagaata
       61 aaataagaac aaaaaaccta tacggtaaag aggagacgac atgaaaaaaa gagtgatcga
      121 atttatattg ctgacggtat ttgccgttct ttttttatca ggctgcaaga aaaatgtagg
      181 aacacccgaa gataatccgg ttgtagaaga agacgaagag tctgtggaac ctgcgaaaga
      241 ttatttgtac ggatacagtt gtgcggatct caaagatccg ttttatatgg ctttgaaaga
      301 tgctatcggg gttcaggcag aacaaaacaa agggcagttt attgtgcgtg atgcggggaa
      361 tgatccggtg aaacaaaatg aacagcttat ggagatgatt gataagggag cagacgttgt
      421 ttttcttacg gcatctgatc ctgatgctgt cacaccggct ttagaagctc tggacaaagc
      481 ggatattccg gttatcttac ttgaaactca agtgaaagaa cgcgacctgg cgaaagcgtt
      541 tatcagttcg gacaattata atgcaggaaa agtgtgcgga gaagatttga aagagaagat
      601 gcctgacgga ggaaagctcg tcattgtgga ggataaggca gaccaaaact taagcgaagg
      661 aatttcagga tttgaagaaa agatcaaaaa cggaggattt gaagttgtta agcgtgttga
      721 cggcgctcag acagatggga tgacaaaaga attgaatgaa attttgtcat caggtacaaa
      781 ggttgaagtt attatgtgtg cagatgatca gatggctgaa gcggttcgcg cgactgtgaa
      841 agagagtgga aaaaacgata ttctcatcta tagtatcggc ggttctccgg cggcaaaggc
      901 agcgcttttg gctcaggaag gagcgatggc gggaattgga gcacgctctc cgattattga
      961 aggaaaaatt gccgtaaaga ccgcagatgc aattttagat gacggttcct atgaagaaga
     1021 ggttttgatc gaaacatttt ttataaataa agagaatgta gagatatacg gaacagatgg
     1081 ctggcagtaa gaaaggaaac ggaatatgag aaaaacagaa ggaagtccgc ttccgttcgg
     1141 agcggctgca aaagaaagaa aagtgaattt ttctgtgcag gttccgatgg gaaaaacgtg
     1201 tgaacttctg ctttacagag aaggaaacac aaagccggaa gtctgctttg acatgccgga
     1261 ggaagaaggc ataggagagg tacgtttcct aagtgtagaa gaaattgatc ctgaaaaata
     1321 cgaatataac tttttgatcg atggaaaggt atgtacagat ccatacgcaa aagaaattgc
     1381 aggaagggaa gtatttggaa aacggcagaa cgatgataaa catggcgtac gggcgaaaat
     1441 acaagtttca gattatgatt ggggcgaaga tcgcccgatt cggcatccat ggaatgaagt
     1501 agtttcatac agtatccatg tgaggggctt tacaaagcac agttcctcaa aagtgctgca
     1561 tagaggaacg tatcaagggg tgatcgaaaa gattccttat ctgaaagaac ttggggtgaa
     1621 ccagatccag tgcatgccga tttatgagtt tgatgagtgt gcaaaaacaa agattaatta
     1681 ttggggatat ggtccggcgt tttattttgc gccgaaggag gcatattctt cggacggatg
     1741 tgctgtgcgc gagttgaaag atatggtaaa agcatgtcac aaggcagaca tagaggttgt
     1801 tttggaaatg ccgtttgctg cgggaatttc cgagcaagaa gcggcggagt gtctgcgctt
     1861 ttacatgatg gagtatcatg tggacgggtt tgtggtcaat ccttatatcg tttcatggga
     1921 acttttaaat aaagatcctt ttttgaaaga tattcgtttg atgcgcaaag atgacgggtt
     1981 tcaaaatgtg atgcgccgtt ttttaaaagg cgatgagaat atggttaatg atgtgatctc
     2041 atcacttggt cacaatagcg cttcggacgg aaaatgtaac tatattacag cgcagaccgg
     2101 ttttacattg agagaccttg tatcatatga ctgcaaacat aacgaagaaa atggagaaaa
     2161 taatacagac ggtcctgact ataattatag ttggaattgc ggcgcagagg gacagagccg
     2221 taaaagaagt gttgtgaatc tgcggaaaag tcagatcaga aatgcgtttc ttttgttgtt
     2281 gttggcgcag ggaacaccgt gtcttttgtc gggagatgaa ttttacaata cgcagaaagg
     2341 aaataataac gtttattgtc aggataatgc aacggggtgg cttgactgga gcaggcttaa
     2401 gtctgacaga ggattgtttg attacgtaaa agcgttgatt acatttcgga aaaatcatcc
     2461 gtgtttgtgt cagaaagaac cgttaaaagg aatggatcgt acggcatgtg gaatgccgga
     2521 tgtctcttat cacggagaaa atgcatggca ggtaaaaaac gaagtgtcaa gccgccagtt
     2581 gggcgttatg tattcaggag caggcgttgg agactgtgat tgctttgtcg cttataatat
     2641 gcactggctt cctcatagtt atgcgcttcc atctccggga aaaggaaaat gctggcattt
     2701 ggcgttggat acggacagag gcgttctgtc aaagagtata aaattggaag atcagaaacg
     2761 tatcgagatc agagagcgga gtattgcagt tcttataagc tgcgaggaag ataaaacaga
     2821 gttacggaat acaaattttc actccaaagg taagaccgcg gagaagaaat ctgtttcgga
     2881 aacagaaaaa acagatatag taacgtctga tgccgaaaaa gaaaagaaat gcagacagga
     2941 gtaaagaata tgaaagcgat caaacatttt caaacgatca caaaacataa aattctcgtt
     3001 atgcaggaat gttttcgcgt aggtttgtat cggcagggac ttcttcatga tttgtctaaa
     3061 tatggatgga cggaatttcg agtcggatgc aggtattatc agggaaacag aagtcccaac
     3121 aatgcggagc gggaagacac cgggctgtcc acatcctgga ttcaccacta tggaaggaat
     3181 aagcatcatt ttgagcactg ggtggactac ggtgtggact gtgacacag
//
LOCUS       DBMDXF010000203         3203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6774_length_3203_cov_2.085429, whole genome shotgun sequence.
ACCESSION   DBMDXF010000203 DBMDXF010000000
VERSION     DBMDXF010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6774_length_3203_cov_2.085429"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(208..2046)
                     /gene="glmS"
                     /locus_tag="ACZPD4_07610"
     CDS             complement(208..2046)
                     /gene="glmS"
                     /locus_tag="ACZPD4_07610"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1378955.1"
                     /translation="MCGIVGFIGEQPAAPILLEGLSKLEYRGYDSAGIAVRNEKTDKI
                     AIVKAKGRLKILAEKTDNGKSVRGTCGIGHTRWATHGEPSENNAHPHCTDDKSVVLVH
                     NGIIENYQELKTKLQKTGYTFYSQTDTEIAVKLIDYYYKKTGTPLEALTRAMLRIRGS
                     YAFGVMFRDCPGRLFAARKDSPLIIGKSKKGCLIASDVPAILDRTRNVYYIGNLEIAE
                     MTSEEVHFYNIDREEIQKEMTEIKWDAESAEKGGYEHFMLKEIHEQPKAVQDTLGAYI
                     KDGAIDFSEAGLTDEQLKSIERIYVAACGSAYHVGVVGKYVTESLAGIPVEVDLASEF
                     RYRNPILAPNSALIVVSQSGETADSLAALRLAKEKGIPVIGIVNVVGSSIARESDHVL
                     YTYAGPEISVATTKAYSAQLAAMYLLAVQMAKVKGRIDEKRHGELLEELCRIPDKIQK
                     ALDDKERIQWFASKYANAKDVFFIGRGIDYAISLEGSLKMKEISYIHSEAYAAGELKH
                     GTISLIEDGILVVGVLTQNDLFEKTLSNMVEVKSRGAYLMGLTTYGNYSVEDTADFSV
                     YVPKTEEYFSASLAIVPLQLMGYYVSVAKGLDVDKPRNLAKSVTVE"
     gene            complement(2244..3197)
                     /locus_tag="ACZPD4_07615"
     CDS             complement(2244..3197)
                     /locus_tag="ACZPD4_07615"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpnC/YadD family protein"
                     /protein_id="HIR1378956.1"
                     /translation="MTGKENREIKNSVFVDLFYEDESAEANEIALFNAIHDEPLPEGT
                     KIRKFRVDNTIYMNFQNDISFDAGGKVIVFGEHQSTINENMPLRSLLYIGRAYERLVP
                     PRSRYKKKLVPLPTPEFYTFYNGKEKWEKEKELRLSDAYIVKDGEPSLELKVKVINIR
                     PEEHHEILEKCQVLKEYSQFMETVQNYQISGVEEPYKKAIKECVEKGILADYLMRKGS
                     EVVNMLLDEYDYETDIEVQREEAREQGRAEGREEGREQGRAEGRKQGKEEGREEGRKE
                     EKRALVQKKLEKGKTISQIADDLEDTEENIAHLIEEFHLRV"
BASE COUNT          737 a          821 c          560 g         1085 t
ORIGIN      
        1 ttaaattcac tataaacaaa cattactttt atcataattt tcacgataca tttttaataa
       61 taggctaaca tctgttcctt gtcaactctc caaaatgtat attttcaaat acaaaatgca
      121 tattttctct tataaatata ttaaaaagag agaatatgca tttttattca gatattaaaa
      181 gtataatttc ttcacacgat gtaaaaatta ttctactgtt acggattttg ccagattccg
      241 cggtttgtca acatccaaac cttttgcgac actgacgtaa tatcccataa gctgaagcgg
      301 cacgatcgca agacttgcgg aaaaatattc ttctgttttc ggcacataaa cggaaaagtc
      361 cgccgtatct tccacgctgt agtttccata tgtcgtaagt cccatcaaat atgcgcctct
      421 gctttttacc tcgaccatat tgctcaatgt tttctcaaac agatcgttct gagttaagac
      481 tcccacgaca agaattccgt cttcgatgag actgattgtt ccatgcttca gctctcctgc
      541 cgcatatgct tctgaatgga tataactgat ctccttcatc ttgaggcttc cttccagact
      601 gattgcataa tcgatgccgc gcccgataaa aaatacatct tttgcattcg catatttact
      661 tgcaaaccac tgtatccgct ctttatcgtc aagcgctttc tgaatcttat cgggaattct
      721 gcacagttcc tcgagcaatt ctccatgccg tttctcatct attctgcctt ttacttttgc
      781 catctgtacg gcgagaaggt acattgccgc caactgtgcg ctgtacgcct ttgtcgtcgc
      841 aaccgagatc tcggggcctg catatgtgta taagacatga tcgctttctc ttgcaatgct
      901 tgagccgacc acattgacga tcccgataac cggaattcct ttctcttttg caagacgcaa
      961 ggcggcaaga ctgtctgccg tctctcctga ctgtgatacg acgatcaacg ccgaattcgg
     1021 cgcaagaatc gggttgcgat aacggaattc agatgcgaga tccacttcta ccggaatacc
     1081 tgccagactt tccgtcacat attttccgac aactccgaca tgatacgccg atccgcatgc
     1141 cgcaacatag atcctctcaa tgctttttag ttgctcgtct gtcagacctg cttccgaaaa
     1201 gtcaatcgca ccgtctttta tataagcgcc cagcgtatcc tgaacggctt tcggctgctc
     1261 atgtatctct tttaacataa agtgttcata cccgcctttt tcggctgact cggcatccca
     1321 cttgatctct gtcatttctt tttgtatctc ttcccggtcg atattataaa aatgcacttc
     1381 ctccgaagtc atctccgcta tttcaagatt gcctatataa tatacgtttc ttgtacgatc
     1441 cagaatcgcc ggaacgtctg acgcgatcag gcagcctttt ttactctttc cgataataag
     1501 cgggctgtct tttctggccg caaacaatct gcccggacaa tcgcggaaca tcactccgaa
     1561 agcatatgat cctcttattc taagcatagc ccttgtaagc gcctcaagag gtgttccggt
     1621 cttcttataa taataatcta tcagttttac cgcgatctcc gtgtctgtct gtgaataaaa
     1681 cgtatatccc gttttctgaa gctttgtctt taattcttga tagttttcga taatcccgtt
     1741 atgcacaaga acaacggatt tatcatccgt gcagtgagga tgtgcattat tctcggaagg
     1801 ttcgccgtga gtggcccatc gtgtatgacc gatcccgcat gtgcctcgca cacttttccc
     1861 gttatctgtt ttttctgcaa ggatcttcaa acgccccttt gcctttacta tcgcgatctt
     1921 atccgtcttt tcattacgaa ccgcgattcc cgccgaatca taccctctgt actccagctt
     1981 tgaaagccct tccaaaagaa tcggcgctgc cggctgttct ccgatgaatc ctacaattcc
     2041 acacattgtt ccacatcctc ctttgatgcc tgtatttata cataaaaatt ttgtgcataa
     2101 tagtaccacc actttttatg aaatgtcaat agaacaaacg aattttttta ttttttttgc
     2161 ttttgttttt atttcttttg ctgtttcggg aaaatctgtc tgcatatggg cgctatccga
     2221 tctcttgata aactgccgcc gctctacacc cgaagatgaa actcttcgat caggtgagca
     2281 atattttctt ctgtatcttc aagatcatct gcgatttgag aaattgtttt ccccttttca
     2341 agttttttct gaacgagagc tcttttttct tcttttcgac cttcttctct gccttcttcc
     2401 ttcccttgct ttcttccctc tgctcgtcct tgttctcgtc cttcctctct gccttctgct
     2461 cgcccttgct ctcttgcttc ttctctctgc acttcgatat ctgtttcata atcatattca
     2521 tccaacagca tattgacaac ctcgcttcct tttcgcatta aataatcagc aagaattcct
     2581 ttttcaacgc actctttaat tgccttttta tacggttctt ccacgccgga gatctgatag
     2641 ttctgtaccg tttccataaa ctggctgtat tctttcagca cctgacattt ctccaggatc
     2701 tcatgatgct cttccgggcg gatattgatg acctttactt ttaattccag actcggttca
     2761 ccgtctttta caatataggc gtctgacaga cggagctctt tttccttctc ccatttttct
     2821 tttccgttat aaaatgtgta aaattccggc gtcggaaggg gaacgagttt ctttttatat
     2881 cggcttctcg gcggcacgag cctttcatat gcccgtccga tgtacaacag actgcgaagg
     2941 ggcatgtttt cgttgatcgt agactgatgc tcgccgaata cgatcacttt cccacctgca
     3001 tcgaaagaaa tgtcgttttg aaaattcata tagatcgtat tgtctacgcg gaatttccgg
     3061 atcttcgttc cttccgggag gggctcgtcg tggatggcgt taaagagcgc gatttcattt
     3121 gcttctgctg attcgtcttc atagaacaga tctacgaaga cgctgttttt gatttctctg
     3181 ttttcttttc ctgtcatagg ctt
//
LOCUS       DBMDXF010000204         3201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6777_length_3201_cov_1.452258, whole genome shotgun sequence.
ACCESSION   DBMDXF010000204 DBMDXF010000000
VERSION     DBMDXF010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6777_length_3201_cov_1.452258"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2075)
                     /locus_tag="ACZPD4_07620"
     CDS             complement(<1..2075)
                     /locus_tag="ACZPD4_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1378957.1"
                     /translation="MEMNFAFLEKKQEYELFSGACIDAERILESSPVMSAVASRKALE
                     LCVKWIYSIDSALEPIGNREGLQSLLHNNGFPSLMDITLWRRLQHIVRNGNESVHTSK
                     RLTRDNAVLSLNILFDFVEWVDYCYGRDYVERVFDEKKIPDATTDMNVVQEEYRQVIK
                     DVQKNADKIVNEKDKEIERLLALNKELRNEMKVQKARNIKNREYVYEPDLSEWTTRKR
                     YIDADLEANGYVFDQDKKRNCVEVEYPVVGMPNQTGRGYVDYVIWGDTGKIIAVVEAK
                     KASESGDKGRNQGKLYADCIQNMQGKRPIIFYTNGFETYLWDDANSAPRLVSGIFPQK
                     DINAMIERRCIKKSVNSIQIDKQITDRWYQLRAVTKCCENYEKGIRKCLLVMATGTGK
                     TRTAASIVDVMTRSELMGKVLFLADRKELVKQAKDAFYKYIENTTMCNLLLNKDERNA
                     KIIFSTYQTMLHAIDTIKNADGSRFFSPGHFSLIVIDEAHRSIFNKYKAIFQYFDACL
                     LGLTATPKNTIHQSTYTFFDMKNNIPTDVYEYEEAVEKDHVLVPFYLIETSTQISDDG
                     ITYADLDEEEREAYEAEFVEDGGVPEHIPPERINKYIFNVDTVDRMISDLMNNGIRHK
                     NGNHVGKTIIFAQNKLHAKFIVDRFNELYPQYKGNFCKLVVCDEPYAGQNLKDFKKAD
                     DYPFITVTV"
     gene            complement(2103..3143)
                     /locus_tag="ACZPD4_07625"
     CDS             complement(2103..3143)
                     /locus_tag="ACZPD4_07625"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIR1378958.1"
                     /translation="MRVKLGDVCERGTSNLKLSDVSEKNGEFSVFGASGYIGSVDFYQ
                     QGYPYVAVVKDGAGIGRAMLCPGKTSVIGTMQYLLPKDNILPKYLFYVVKYMNLEKYF
                     TGATIPHIYFKDYKNEEFNFDFWERQVEIVSVLSKCEKVIDLCKQELQLLDKLIKARF
                     VEMFGDVIHNSKKWQVCLFAEITSSRLGKMLDAKQQTGRNSYPYLANFNVQWFRFNLE
                     NLNKMDFDEKDRAEFELREGDLLVCEGGEIGRCAVWHNELQPCFFQKALHRVRCNHQI
                     ILPDYLAWWFRYNCDYGGFSALAGAKATIAHLPGAKLKQLQVAVPPMELQEQFAVFVA
                     QTDKSKVAVRKY"
BASE COUNT          939 a          758 c          413 g         1091 t
ORIGIN      
        1 tctacagtca ccgtaataaa cggatagtca tccgcttttt taaaatcctt aagattttgt
       61 cctgcatatg gctcatcaca aaccaccaac ttgcaaaaat tacctttata ttgcggatat
      121 aattcattaa atcgatcaac aataaatttt gcatgcaatt tattttgtgc gaaaataatt
      181 gtcttcccga catgatttcc atttttatgt ctaatgccgt tattcatcaa atctgaaatc
      241 attctatcaa ccgtatcgac attaaaaata tatttattta ttctctccgg cggaatatgt
      301 tcaggcactc ctccatcttc gacaaactct gcttcatatg cctccctctc ttcctcgtcc
      361 aaatccgcat atgtaattcc atcatctgaa atctgtgtcg aagtctcaat taaataaaac
      421 ggaactaaca catgatcttt ttccaccgct tcctcatact catatacatc cgtcggtata
      481 ttatttttca tatcaaaaaa tgtataggta gactgatgaa tcgtattctt cggcgtcgca
      541 gtcaatccca gcaggcaagc gtcaaaatat tgaaaaattg ctttatattt attaaaaata
      601 cttctatgtg cttcatctat tacaattaac gaaaaatgcc ccggcgaaaa aaatcgcgat
      661 ccatccgcat tttttatagt atcaatcgca tgtaacattg tctggtatgt tgaaaatata
      721 atttttgcat ttcgctcatc tttatttagt aaaagattac acattgttgt attctctata
      781 tatttataaa acgcatcctt tgcctgcttc accaattctt tgcgatctgc caaaaacaac
      841 acttttccca ttaactcact gcgcgtcatc acatctacta tcgatgccgc tgttcgtgtt
      901 ttccctgtac ccgtcgccat tacaagcaaa cattttcgga ttcccttttc ataattttca
      961 cagcattttg taactgctct taattgatac cagcgatccg taatttgctt atcaatctga
     1021 atgctgttaa ccgatttctt tatacatcgc cgctcaatca ttgcattgat atctttttgc
     1081 ggaaagatgc cgctcacaag tctgggcgca ctgtttgcat catcccaaag ataagtttca
     1141 aaaccatttg tataaaatat aatcggtctt tttccctgca tattttggat acagtctgca
     1201 tataatttcc cttgattccg ccctttatct ccgctctcgc ttgccttttt tgcttctact
     1261 acagcaataa tctttcctgt atctccccat ataacataat ccacatatcc ccgtcctgtt
     1321 tgattcggca ttccgacaac aggatattca acttccacac aatttctctt tttatcctga
     1381 tcgaacacat atccattcgc ctctaaatct gcatcaatat atctttttct cgtcgtccat
     1441 tctgacagat ccggttcgta aacatattcc cgatttttta tattacgggc tttttgaact
     1501 ttcatctcat tacgaagttc tttattcaaa gctaaaagac gttctatttc tttatctttt
     1561 tcattcacta ttttatcagc gtttttttga acgtctttaa taacctgccg atactcttcc
     1621 tgtaccacat tcatatccgt tgtcgcatca ggaatctttt tctcatcaaa tactcgttca
     1681 acataatctc ttccgtaaca ataatcaacc cattccacaa aatcaaataa tatgttgaga
     1741 gaaagaactg cattgtctcg cgtcagtcgt tttgaagtat gtacagactc atttccattc
     1801 cgaacaatgt gctgaagcct tctccacaat gtaatatcca tcaaagaagg aaagccatta
     1861 ttgtgaagta aagactgcaa tccttctcta tttccaatcg gttctaatgc agaatcaatc
     1921 gaataaatcc atttcacaca caattccaat gcttttcttg aagctaccgc gctcatcacc
     1981 ggcgaagatt ctaatattct ttccgcatca atacacgctc ctgaaaacaa ttcatattct
     2041 tgtttcttct caagaaatgc aaaattcatt tccatgataa gctcctcctc tcaagcaaat
     2101 ttttaatatt tccgcactgc aacttttgat ttgtcggttt gagcgacgaa gacagcaaat
     2161 tgctcttgta attccattgg aggtacggcg acctgtaact gctttaattt tgcaccaggc
     2221 aaatgtgcaa tagtagcttt tgcaccggct aatgcagaaa aacctccata atcgcaatta
     2281 tatctaaacc accacgccag ataatctggt aaaattatct ggtgattaca tctcactcga
     2341 tgcaacgcct tttggaaaaa acatggctgt aactcattat gccacacagc acaccgtcca
     2401 atttcgccac cctcacaaac caaaagatct ccttcacgca gctcaaactc tgcacggtct
     2461 ttttcatcaa aatccatttt atttagattt tcaagattga accgaaacca ctgaacattg
     2521 aaattggcta agtaaggata actatttctt ccagtttgct gttttgcatc tagcattttc
     2581 cccaatcgtg aagaagtgat ctctgcaaat aaacaaactt gccatttctt gctgttgtgg
     2641 attacatctc caaacatctc gacaaatcgg gctttgataa gcttgtccaa taattgtaat
     2701 tcctgtttac acaaatcaat aactttttcg catttactaa gaacacttac aatttcaact
     2761 tgccgttccc aaaaatcaaa attaaattct tcgtttttat aatctttaaa atatatatgc
     2821 gggatggttg cgcctgtaaa atatttttcc aaattcatat attttactac ataaaaaaga
     2881 tactttggca atatattgtc tttcggtaaa agatactgca tcgtacctat tacagaagtt
     2941 tttcccgggc ataacattgc ccttccaatt ccagctccat cctttacaac tgcgacatac
     3001 ggatatcctt gctgataaaa atctacactt ccaatatatc ctgatgcacc aaaaactgaa
     3061 aattccccat tcttttcaga tacatctgac agttttaaat ttgatgtccc acgttcacag
     3121 acatctccta gttttactct catatatcat ccccacaaat cttaatttgc ttgagggata
     3181 catttcactt taagacatat a
//
LOCUS       DBMDXF010000205         3197 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6788_length_3197_cov_2.398579, whole genome shotgun sequence.
ACCESSION   DBMDXF010000205 DBMDXF010000000
VERSION     DBMDXF010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6788_length_3197_cov_2.398579"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>3197
                     /locus_tag="ACZPD4_07630"
     CDS             <1..>3197
                     /locus_tag="ACZPD4_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HIR1378959.1"
                     /translation="ENVKLVQGEINQKGTDKVAALAKTSQADTLIKDVYVRDMSVTGQ
                     SNVAGLVASMNKTTVEECSVNATVNGKRAGGFAAEILGDSVVKNSYARRTADKETFAA
                     TEGDLQGGFAAVIKKSELINNFSELTLSQKAEEKPEETPKKSSEKAAKTACMVGNFVA
                     ESGVGSEAVTKAEHNISFGPKEYSFAGNSTAENVLTNYTENYEYTGSVSNDEGTQTPE
                     HTGKIDKATAAQITNKTFYIDTLKWDEKIWYLDDVAGGKRPRLKAEGDVYGAEEEDTS
                     KGEDSEGDSNNASSQEVVYIQAPVQDEETEEPQMAGETPAEVISFIAQKNAEEIAAMD
                     SLESVKDYQADRKLIYENLRLFMPFYTYEQIVKDGNKVDPSHVLNKKTVLAVYPMDDR
                     GNRIVALSDKTVQDIKKIRIQFTDETTPLIYNITYIDTRENIASYKVAQIPVHFNFRN
                     YVVSTTTTQFNKLLTAAKGYDFDGDIETRVSQKDSASVLSVYRKNYNNVVKSEMEQVL
                     ISMAASNPQYPVNTTNKAAELMVEENLIANGYLKDFLYAYNYIDRWYDFQIGGINIRD
                     VVLFDNSILKSGKSPRNLVAEIVQLSSSGGRQGNSTPSFYINRISQYTGISNVASFVE
                     YFMTAYAGYTDVNDWIIDNFQGGFIVEARANNPKIDSRLWRILKNNTVQRNNELILPV
                     LSYKTSKNLYLASFPTSLVYGNLQIYNGYQNTDEWRQQKKQQVINQINDFKTSYDNFV
                     DVAENGAQSINKSKFLIVDSSANKDHSQDVFQEFYKPLQTMWKSNNGAVAVIFGNPNY
                     DYIYYNSSNFIGDLTVLNHEMGHVTDMWIWMENKGKRPGRNGEDYSNGYANQANVDYN
                     MNFMKTYARDGSMVTNLTPDRINTQEEFKSYYKEVFETIYTLDYLQGKAYLELTPAQQ
                     SAITSQHRYGTTNNYQSRNQSNSTWRTIGAAELENMNLKTLDDLWDNQLTIRPGHRFD
                     LRSMNDVGVNNLGAYQIDRVCYASWYVPYVDGGTPNAQTFRRNGYELGGLYGYSDGLV
                     EYLSNRTQTGDLAYFKKKMQDENFSFETYRKNKNK"
BASE COUNT         1097 a          577 c          782 g          741 t
ORIGIN      
        1 ttgaaaacgt gaagcttgta caaggcgaga tcaatcagaa gggaacagat aaagttgcgg
       61 ctctggcaaa aacttcacag gcagacactc ttatcaaaga cgtatatgtc agagatatga
      121 gcgttacggg acaaagtaat gtagcaggtc ttgttgcaag tatgaataag acaactgtag
      181 aagaatgttc tgtcaacgcc acagtaaacg gaaaacgtgc aggtggattt gcggcagaga
      241 ttttaggaga ttcagtcgtt aaaaacagtt atgcaagaag aacggcggat aaagagacat
      301 tcgcagcaac tgaaggcgac ctgcagggcg gatttgcggc tgtcattaaa aagtcagagc
      361 tgatcaataa cttcagtgag cttacattaa gtcaaaaggc agaggaaaaa ccggaagaga
      421 caccgaaaaa atcatctgaa aaagcggcga aaacggcatg tatggtcggt aatttcgttg
      481 cagaaagcgg agtaggatca gaagcggtta caaaagcaga gcacaatatt tcctttggtc
      541 cgaaggaata ttcttttgca ggaaacagta cggcggaaaa tgttttaacg aactatacag
      601 agaactacga gtatacagga agcgtttcaa atgatgaggg aactcaaact cctgaacata
      661 caggaaaaat cgataaagcg acagcagcgc agattacaaa taaaaccttc tatatagata
      721 cacttaaatg ggatgagaag atctggtatc tggatgatgt ggcaggtgga aaaagacctc
      781 gtttaaaggc agaaggtgat gtttacggag ctgaggaaga agatacttca aaaggagagg
      841 actcggaagg agattcaaac aacgcgtctt cgcaagaagt agtttacata caggcgccgg
      901 tacaggatga agaaacggaa gagccgcaga tggcaggaga gactccggca gaagtgatca
      961 gttttatcgc gcagaaaaat gcagaagaga ttgcggctat ggacagtctg gaatctgtaa
     1021 aggattatca ggcagatcga aaactgattt atgaaaactt gcggttgttt atgccgttct
     1081 atacatatga acagatcgtc aaagacggaa ataaggttga tccgtcgcat gtattaaata
     1141 aaaagacggt gcttgcggtc tatccgatgg atgaccgtgg caacagaatt gtggcattat
     1201 ccgataagac agttcaggat ataaaaaaga tccgtattca gtttacagat gaaacaacac
     1261 cgttgatcta taatattaca tatattgata caagggaaaa tattgcttcg tacaaagtag
     1321 cgcagatccc tgttcacttt aactttagaa actatgttgt cagtacgaca acaacgcagt
     1381 ttaataaact attgactgcg gcaaagggat atgattttga cggagatatt gaaacgagag
     1441 tttctcaaaa agacagcgct tctgtattgt cggtataccg gaaaaattat aacaatgttg
     1501 tcaaatcaga gatggagcag gtgcttattt ccatggcggc atcaaatccg cagtatccgg
     1561 taaatactac aaataaagca gcagaactga tggtagagga aaatcttatc gcaaatggat
     1621 atctgaagga cttcctctat gcgtataact atatagaccg ttggtacgat tttcagatcg
     1681 gcggtattaa tataagagac gttgtgcttt ttgacaactc gatcttaaaa tcgggcaaat
     1741 ctccgcgcaa tcttgtggca gagatcgtac agcttagcag cagcggggga cgtcagggaa
     1801 atagtacgcc gtccttctac atcaaccgga tctcacagta tacgggaatt tcaaacgtcg
     1861 cttctttcgt agaatatttt atgactgcat acgcaggata cacggatgta aacgactgga
     1921 tcattgataa tttccagggc ggattcatcg tagaagcaag agcgaataat ccgaagatcg
     1981 attccagatt atggagaata ctgaaaaaca atacggtaca gagaaacaac gagctgatcc
     2041 ttccggtatt gtcttataag acatcgaaga acttgtatct ggcatcgttc ccgacatctc
     2101 ttgtatatgg aaacttgcag atttacaacg gatatcagaa tacagatgag tggagacagc
     2161 agaagaagca gcaggtgatc aatcagatca atgatttcaa gacaagctat gataacttcg
     2221 tggacgttgc agaaaacgga gcacagtcaa ttaataaaag taaattcctg atcgtcgatt
     2281 cctctgcaaa taaagatcac agtcaggatg tatttcagga attttacaaa ccgctgcaga
     2341 cgatgtggaa gagcaacaac ggagcggtag ccgtaatatt cggaaatccg aattatgatt
     2401 acatttatta taacagctct aactttatcg gagatctgac cgtcttaaac catgagatgg
     2461 gtcatgtgac agatatgtgg atctggatgg agaataaagg aaaacgtccg ggacgtaacg
     2521 gagaggacta ttctaacgga tatgcgaacc aggcaaacgt cgattacaac atgaacttta
     2581 tgaagacgta tgcaagagac ggctcgatgg tgacaaacct gactccggac agaatcaata
     2641 cacaggaaga atttaaaagc tattacaaag aagtgttcga gacgatctat acgttggatt
     2701 atcttcaggg caaagcgtac ctggagttga cgcccgcaca gcagtcggcg atcacatcgc
     2761 agcacagata tggaacgacg aacaattacc agagtcggaa tcagtcaaac agtacatgga
     2821 gaacgatcgg cgccgccgag cttgagaata tgaatcttaa gacgttggac gatctttggg
     2881 ataatcagct gacgatcaga ccgggacatc ggttcgatct tagaagtatg aacgatgtcg
     2941 gcgtgaataa tctcggggca tatcagatcg acagagtgtg ctatgcaagc tggtacgtgc
     3001 cttacgttga cggaggaacg ccgaatgccc agacatttag aagaaacgga tacgaactcg
     3061 gcggactgta cggatacagc gacggtcttg tagagtattt aagtaaccgt acacagacag
     3121 gagatcttgc atatttcaaa aagaaaatgc aggatgagaa tttctccttt gaaacataca
     3181 gaaagaataa aaataaa
//
LOCUS       DBMDXF010000206         3170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6860_length_3170_cov_1.825676, whole genome shotgun sequence.
ACCESSION   DBMDXF010000206 DBMDXF010000000
VERSION     DBMDXF010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6860_length_3170_cov_1.825676"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(195..1469)
                     /locus_tag="ACZPD4_07635"
     CDS             complement(195..1469)
                     /locus_tag="ACZPD4_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1378960.1"
                     /translation="MSDYRIETKCIQSGYEPGNGEARVLPIYQSTTFRYTSSEQMGRL
                     FDLEESGYFYTRLQNPTNDAVAAKICDLEGGYAGMLTSSGQAANFYAIMNIAEQGDHI
                     VCASALYGGTYNLYAHTIKKMGVDATFVDPDCSEEELEAAFRENTKAVFGESIANPAL
                     VVLDFQKFASAAHRHGVPFIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAACVGG
                     AIIDSGNFDWNAHADKFPGLTTPDKTYHGIVYTEKFGKGAYITKATAQLMRDLGSIQA
                     PQNAFLLNIGLETLHLRVPRHCENALKVAKYLKGHDKVAWVSYPSLEGDKYYDLAQKY
                     MPNGTCGVLTFGLKGGREAAVEMMDKLKMVAIVTHVADARSCVLHPASHTHRQMNEQE
                     LKEAGVQPDLIRFSVGIENAEDIIADVEQALK"
     gene            complement(1524..2255)
                     /locus_tag="ACZPD4_07640"
     CDS             complement(1524..2255)
                     /locus_tag="ACZPD4_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1378961.1"
                     /translation="MSGHSKFANIKHKKEKNDAAKGKIFTKIGRELAVAVKEGGGPDP
                     ANNSRLRDVIAKAKSNNMPNDTIERNIKKAAGEGAGDNYEHITYEGYGPNGTAIIVKT
                     LTDNKNRTASNVRNAFTKGSGNVGTPGCVSFMFDEKGQIFVAKEDCSMDADELMMLAL
                     DAGAEDFAEEEESFEILTDPAAFSEVRLKLEEAGIVMAGAEVTMIPQTYVDLTSEEDI
                     KNIQKTLDLLEEDDDVQDVYHNWNE"
     gene            complement(2366..2710)
                     /locus_tag="ACZPD4_07645"
     CDS             complement(2366..2710)
                     /locus_tag="ACZPD4_07645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1378962.1"
                     /translation="MIRRGDIFYADLSPVVGSEQGGVRPVLVIQNNVGNRHSPTVICA
                     AITSKMNKAKLPTHIEISTRDYKIVKNSVILLEQIRTIDKQRLKEYVCHIDSAMMQKV
                     DKAIRISLEIAT"
     gene            complement(2815..>3170)
                     /locus_tag="ACZPD4_07650"
     CDS             complement(2815..>3170)
                     /locus_tag="ACZPD4_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1378963.1"
                     /translation="SYGGTFVAPEKMKIATIPVGYGDGYPRSLSNKGSVLIRGKRAKI
                     VGRVCMDQFMVDVTDIPGAAFMDRVVLIGKDGKDEITVEELSKLSGRFNYEFVCCLGK
                     RIPRVYTGGDFDLMK"
BASE COUNT          794 a          824 c          509 g         1043 t
ORIGIN      
        1 tccgatatct ttaaaatcag tatgaaaaaa atcgagatat tttatgcagt tttggaggaa
       61 acaattttcc aaaaaacaca tttcagaatt gtaggcagtc accaaagtat aggtggggaa
      121 caaactttca ctcgtcgtct atacaaaaaa tggcacagat gtaaattgta tacatccata
      181 ccatttttta agaattattt taatgcctgc tctacatcgg caataatatc ttcggcgttc
      241 tcgattccta cactgaaacg gatcaaatcc ggctgaactc ccgcctcttt caactcctgc
      301 tcgttcatct gacgatgagt atggcttgcc gggtgaagca cacagcttct tgcgtccgcc
      361 acatgggtta cgattgctac cattttcaat ttatccatca tttccacagc tgcttctctt
      421 ccgcctttca gaccgaatgt cagcactccg cacgtaccgt ttggcatata tttctgtgcc
      481 agatcataat acttatctcc ttccaacgac ggataactta cccaagctac tttatcatgt
      541 cctttcaaat actttgcaac tttcaacgca ttctcacaat gtcttggcac tcttaagtga
      601 agcgtttcaa gtccgatatt cagcaaaaat gcattttgcg gcgcctgaat ggaaccaagg
      661 tctctcataa gctgagctgt tgcctttgta atataagcgc cttttccgaa tttttctgta
      721 tatacaattc cgtgatatgt cttatccggt gtcgtaagtc ccggaaattt atctgcatgg
      781 gcattccagt caaaattgcc actgtcaatg atcgcacctc cgacacacgc cgcatggcca
      841 tccatatatt ttgttgtaga gtgggtaaca atatctgctc cccactcaaa cggacggcag
      901 ttaatcggtg ttgcaaacgt attatccaca ataaacggaa ctccgtgtct gtgcgccgca
      961 cttgcaaatt tttgaaaatc aagaacaaca agagccggat tggcaatcga ctctccaaac
     1021 accgcttttg tgttttctct aaatgcagcc tccagttctt cctcagaaca atccggatct
     1081 acaaatgtag catctacgcc cattttttta attgtatgag cataaagatt atatgtaccg
     1141 ccatacaaag ccgacgcgca tacgatatgg tctccctgtt ccgcaatgtt cataattgca
     1201 tagaaattcg ccgcttgtcc ggaagatgtc agcatccccg catatccgcc ttcaagatcg
     1261 catattttgg cagcaaccgc atcgttcgtc ggattttgaa gacgcgtata aaaatatccc
     1321 gactcttcaa gatcaaacag tcttcccatc tgctcgctgc tcgtataacg aaatgtagtg
     1381 ctctgataaa tcggaagtac tcttgcttct ccgtttccgg gttcatatcc cgactgtata
     1441 cattttgttt cgattctgta atcgctcatc ttctttgtcc tcctgcgttt ttcctgtttg
     1501 tatgcctgca ttccctgaat cgcctattca ttccagttat gatatacgtc ctggacatca
     1561 tcgtcttctt cgagaagatc taatgttttc tggatatttt taatatcctc ttcacttgtc
     1621 aaatcgacat aagtctgagg gatcatcgta acttccgctc ctgccattac aattcccgcc
     1681 tcttctaatt tcagacgcac ttcactaaat gcagccggat ctgtcaaaat ttcaaaactt
     1741 tcctcttctt ccgcgaaatc ttccgctccc gcatccagtg caagcatcat cagttcatcc
     1801 gcatccatgc tgcagtcttc ttttgccaca aaaatctggc ctttctcatc aaacataaat
     1861 gatacacaac ctggcgtgcc tacgtttcca cttccttttg taaacgcgtt gcgtacattt
     1921 gaagctgtcc ggtttttatt atctgtcaat gtttttacga tgattgccgt tccgttcgga
     1981 ccatatcctt catatgtaat atgctcgtaa ttatcgcccg ctccttcgcc tgctgctttt
     2041 ttaatatttc tctcgatcgt atcattcggc atattattcg actttgcctt tgcaataaca
     2101 tcgcgcaaac gactgttatt tgccggatcc ggtccgccgc cttcttttac cgccacagca
     2161 agttctcttc cgatctttgt aaaaatcttt cccttagccg catcgttttt ttctttctta
     2221 tgtttaatat ttgcaaactt tgaatgtcca gacatataaa ttttctcctc ttctttttct
     2281 cttgtacagt ccgtttcgct caaccgcaca ttttgcttta caattttatc actcttttga
     2341 tcttctgtaa agaagtcaga acagtctatg tagcaatttc aagactgatc cttattgctt
     2401 tatctacttt ctgcatcatt gcactgtcta tatgacagac atattctttc agccgctgct
     2461 tatcgatcgt acgaatctgt tctagcaata taacagaatt ttttacgatt ttataatctc
     2521 ttgtactgat ctctatatga gtcggcagct tcgccttatt catctttgat gtaatcgccg
     2581 cacagatcac agtcggacta tgtctgtttc cgacattatt ctgtatgaca agaacaggtc
     2641 ttacccctcc ctgttccgaa ccgacaacag gacttaagtc ggcataaaaa atgtctccgc
     2701 gtctgatcat caaatttttc actctccgat ttcatactaa tattctcctc ataacctccc
     2761 ggtcatgttc atttatcagt attccctttg tcatcggatg tattcaccaa acaattactt
     2821 catcagatca aaatcaccgc ctgtgtaaac cctcggaatc cgttttccga gacaacagac
     2881 aaattcatag ttaaatcttc cgcttaattt tgacaattcc tctactgtta tttcatcttt
     2941 tccgtctttt ccgatcaaaa caacccgatc cataaatgcc gcccccggta tatcggtcac
     3001 atctaccatg aactggtcca tacatactct tcctacgatt tttgcccgct ttcctctgat
     3061 aagcacactg cctttattag aaagagaacg tggatatccg tctccgtatc cgaccgggat
     3121 cgtggcaatt ttcatctttt ccggagcaac aaatgttccc ccgtagctaa
//
LOCUS       DBMDXF010000207         3163 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6880_length_3163_cov_1.366101, whole genome shotgun sequence.
ACCESSION   DBMDXF010000207 DBMDXF010000000
VERSION     DBMDXF010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6880_length_3163_cov_1.366101"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /gene="jag"
                     /locus_tag="ACZPD4_07655"
     CDS             <1..364
                     /gene="jag"
                     /locus_tag="ACZPD4_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR1378964.1"
                     /translation="DGALYVDMKGENMGILIGKRGQTLDSLQYLANRVANKHQSGYVR
                     VKLDTENYRARREETLKHLAKNIAHKVKRNRRPVILEPMNPYERRIIHSTLQSDPYVT
                     THSEGEEPYRKVVVTLKR"
     assembly_gap    461..470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            544..1941
                     /gene="mnmE"
                     /locus_tag="ACZPD4_07660"
     CDS             544..1941
                     /gene="mnmE"
                     /locus_tag="ACZPD4_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1378965.1"
                     /translation="MRKETIAAISTGMTNSGIGIVRISGEEAVLVADRIYRGKKKLCE
                     VKSHTINYGYIVDREISSAEKKSEEVIDEVLVTVMKAPGTFTGEDTVEINCHGGTFVV
                     KKVLELVLKNGASPAEPGEFTKRAFLNGKMDLSQAEAVIDVINSENEYALQSSVSQLK
                     GSVKKKINDIREKIIYHTAFIETALDDPEHISVDGYSDTLRSSAEEIIQELERLIHSA
                     DDGRVIKEGIKTVIVGKPNAGKSSLLNVLAGHERAIVTDIEGTTRDVLEETIKLGVLN
                     LNVVDTAGIRQTEDLIEKIGVDKALEYAETADLIIYVVDASRSLDENDEKIINMISDK
                     KSIVLLNKSDIDTVISAEHIKEKVSNIPIISISAKEERGIKDLEDKVKEMFLKGDISF
                     NDQVYISNVRQKNALLEALESMKKVIRSIDDNMPEDFYSIDLMDAYESLGYITGNSVG
                     EDLINEIFSKFCMGK"
     gene            1907..>3163
                     /gene="mnmG"
                     /locus_tag="ACZPD4_07665"
     CDS             1907..>3163
                     /gene="mnmG"
                     /locus_tag="ACZPD4_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1378966.1"
                     /translation="MKYSANSVWENKDQYDIAVVGAGHAGCEAALACARLGFKTVMFT
                     VSVDSIALMPCNPNIGGSSKGHLVKEIDALGGEMGKVIDKTFIQSKMLNQSKGPAVHS
                     LRAQADKSDYSKEMRRVLEEQDNLDIRQMEVTDIIADDPNESGIRKIKGVQTYSGAIY
                     PCKAVILCTGTYLKARCIYGEISTQTGPNGLQSANYLTDSLKSLGIKMYRFKTGTPAR
                     IDKNSIDFTKMEEQKGDERVVPFSFTTDPEDVQIDQVSCWLTYTNETTHEIIRENLDR
                     SPLFSGAIEGTGPRYCPSIEDKVVKFPDKNRHQVFIEPEGIHTNEMYVGGMSSSLPED
                     VQYAMYRSVPGLEHAKIVRNAYAIEYDCIDARQLKSTLEFKNIEGLFSGGQFNGSSGY
                     EEAAAQGIVAGINAARKLQGKEGIVID"
BASE COUNT         1143 a          465 c          771 g          774 t
ORIGIN      
        1 agacggagct ctttatgttg atatgaaagg tgagaatatg ggaattctta tcggtaagag
       61 aggacaaact cttgattccc ttcaatatct tgcaaaccgc gttgcaaaca aacatcagtc
      121 aggttatgta agagtgaaac tcgatacaga aaattatcgt gcaagaagag aagagacatt
      181 aaaacatctt gcgaaaaata ttgcacataa ggtaaaaagg aacagaagac ctgttatact
      241 tgagccgatg aacccgtatg aaagaaggat tattcattcc acactgcagt cagatccata
      301 tgtaacgaca cacagtgaag gtgaagagcc gtacagaaaa gttgtagtta cattaaaaag
      361 ataattaaat aaaataaaag acgtctgata gtttgcggat atcgtgaaac gtcaggcgtt
      421 tttttgtgaa taaataagtg gataaataag ctgacgttta nnnnnnnnnn taaaatgtaa
      481 tgctttattt ttagtttgcg ggaagttaag tattaaatta agaaatcggg agaacgggat
      541 aaaatgagaa aagaaacgat cgcagccata tcgaccggaa tgacaaactc gggaatagga
      601 attgtgagaa tcagcggaga ggaagctgtt ttagttgcag accgtattta cagaggtaag
      661 aaaaagcttt gtgaagtaaa aagccatacg ataaattatg gatatattgt agacagagaa
      721 atatcttcgg cagaaaagaa atcggaagaa gtaatagacg aggttcttgt aactgtgatg
      781 aaagctcccg gaacatttac aggagaagat accgtggaga taaattgtca cggcggaaca
      841 tttgttgtaa aaaaagtgtt ggaactcgtg ttaaaaaacg gggcaagtcc tgccgaaccg
      901 ggagaattta caaagagggc atttttaaac ggaaagatgg atctttcaca ggctgaagcg
      961 gttatagatg taatcaattc cgaaaacgaa tatgcacttc aaagttctgt cagccagcta
     1021 aaaggaagcg taaaaaagaa gattaatgat ataagagaaa aaattattta tcatacggca
     1081 tttatagaga cagcgcttga tgatccggag catatcagcg tagatggata ttccgatact
     1141 ttgcgcagtt cggcggaaga gattattcag gaattggaac gactgattca ttcggcagat
     1201 gacggtcgcg tgataaaaga aggaatcaaa acggtgattg tcggaaaacc gaacgcagga
     1261 aaatcgtcac ttttgaatgt tcttgcagga catgagagag caatcgtaac agatatagaa
     1321 ggaacaacga gagatgtatt agaagagacg atcaaattag gcgtactcaa cttaaatgtt
     1381 gtcgatacgg caggaatccg ccagacagaa gatctgattg aaaaaatcgg cgtagataaa
     1441 gcgttagaat atgcagagac agcagatctg atcatttatg tagtcgatgc atcgagaagt
     1501 ctggatgaaa atgatgagaa gatcattaat atgatcagtg acaaaaagtc gattgtttta
     1561 ttaaataaat cagatatcga cactgtgatt tcggcagaac atataaaaga aaaagtttca
     1621 aatattccga tcatctcaat atctgcaaaa gaagaaagag gaatcaaaga cctggaagat
     1681 aaagtgaaag agatgttttt aaaaggcgac atttcattta atgatcaagt gtatatatca
     1741 aatgttcgtc agaaaaacgc tctgttggaa gctctcgaaa gtatgaaaaa ggtaataaga
     1801 agtatcgatg ataatatgcc ggaagatttt tattcgatcg atttgatgga tgcgtatgaa
     1861 agtctcgggt atattacggg aaattcagta ggagaagact tgataaatga aatattcagc
     1921 aaattctgta tgggaaaata aagatcaata tgatattgcg gttgtaggag cgggacacgc
     1981 aggatgtgaa gcagcgctgg cgtgcgcccg gttaggattt aaaacggtaa tgtttacggt
     2041 aagtgtagac agtattgcat tgatgccgtg taatccgaat atagggggaa gttcaaaagg
     2101 acatcttgta aaagaaatcg atgccctcgg cggagaaatg ggaaaggtga tcgacaagac
     2161 atttatacag tcgaaaatgt taaatcagtc aaaaggtccg gcagttcact ctcttcgtgc
     2221 acaggcagat aaatccgact atagcaaaga gatgcggagg gttttggaag aacaggacaa
     2281 tcttgatatc cgtcagatgg aagtgacgga tattatagca gacgatccga atgaatctgg
     2341 aattcggaaa ataaaagggg ttcagacata ttcgggggct atatatccgt gtaaagccgt
     2401 tattttgtgt acaggcacat atttaaaagc ccgctgcatt tacggagaaa taagtacgca
     2461 gacaggtcct aacgggctgc agagcgcaaa ttatcttacc gacagtttga aatcacttgg
     2521 aattaagatg tatagattta aaacgggaac tcctgcaaga attgataaaa actcaatcga
     2581 ttttacaaag atggaagaac aaaaaggtga tgaaagagtc gtgccttttt cttttacgac
     2641 agatccggaa gacgttcaga tcgatcaagt gtcatgctgg cttacatata caaacgaaac
     2701 gacgcacgaa atcattcggg aaaatctgga cagatcgccg ctgttttccg gtgcgattga
     2761 aggaacagga ccgagatatt gtccgtcgat agaagataaa gtagtaaaat tcccagataa
     2821 aaacagacat caggtcttta ttgagccgga aggaatccat acaaatgaaa tgtatgtcgg
     2881 cggaatgtcg agttcattac cggaagacgt tcagtatgca atgtatagaa gtgttccggg
     2941 tttggaacat gcaaagatcg taagaaatgc atatgcgatc gagtatgatt gtatagatgc
     3001 aaggcagtta aaaagtacgt tggaatttaa aaatatagaa ggattattca gcggcgggca
     3061 gtttaacgga agttcgggat atgaagaagc tgcggcacag ggaatcgtag ccggaattaa
     3121 tgcggcaaga aaattgcagg gaaaagaagg aattgtcatt gac
//
LOCUS       DBMDXF010000208         3159 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6883_length_3159_cov_1.547417, whole genome shotgun sequence.
ACCESSION   DBMDXF010000208 DBMDXF010000000
VERSION     DBMDXF010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_3159_cov_1.547417"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            163..516
                     /gene="rsfS"
                     /locus_tag="ACZPD4_07670"
     CDS             163..516
                     /gene="rsfS"
                     /locus_tag="ACZPD4_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1378967.1"
                     /translation="MTQSKKMARIAYDALNEKKGEDIKIIDIAEVSTLGDYFVIANGN
                     SSSQVTALVDNVEEEMHKAGFSLRQREGRASGSWILLDFGDVIIHVFDKESRDFYNIE
                     RAWKDGKEITVKDLD"
     gene            637..960
                     /locus_tag="ACZPD4_07675"
     CDS             637..960
                     /locus_tag="ACZPD4_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1378968.1"
                     /translation="MDKESVLQKRNRAEHSSLLIVSAGFLLLCTCEVFGIITGKLWIG
                     QREIVFGGTLFFIMFLSQFALNLKYYLIDKKKWRIAEGILLIFCIGLTIFRILNPGHY
                     LMEFL"
     gene            969..1172
                     /locus_tag="ACZPD4_07680"
                     /pseudo
     CDS             969..1172
                     /locus_tag="ACZPD4_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1356..2942
                     /locus_tag="ACZPD4_07685"
     CDS             1356..2942
                     /locus_tag="ACZPD4_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1378969.1"
                     /translation="MLRISQLKLPVEHTEAQLKKKLLKTAHIKEQDLKQFFIRKRSVD
                     GRKKPELYYVYTVDLIVANEEHVKKISKGKLQTVQEKNYHIPEHGAKKLRKRPVVIGS
                     GPAGLFCAYLLAFEGYEPLVIERGAPVRERQKVVEQFWKDGILNTASNVQFGEGGAGT
                     FSDGKLNTLVKDKFGRNRFVLETFVKFGAPEEILWESKPHIGTDILIEVVERMREEII
                     RLGGEFLFHSQVTDILPKENCLIVNGCHRIETDAAVFAVGHSARDTFQMLFDRNVAMK
                     SKSFAIGVRAEHRQDMIDEAMYGRKERGPLGAAAYKLTAQLENGRGVYTFCMCPGGYV
                     VNASSEQGRLAVNGMSYHDRAGENANSAVIVTVTKDDYGSDHPLAGMEFQRRLEKKAW
                     EEGGGNVPIQRFADFCTGTISVKTGNVTPNIKGKYVFGNVRNIFPPELAESIESGIRA
                     MGKKIKDFDHDDVILSGVESRTSSPVKIFRKEDFTSSFPWIYPCGEGAGYAGGITSAA
                     MDGMKVAEAIIKTFSAPDSV"
     gene            3044..>3159
                     /locus_tag="ACZPD4_07690"
     CDS             3044..>3159
                     /locus_tag="ACZPD4_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HIR1378970.1"
                     /translation="MEKINTIKEMYKEDRPYEKCEKFGAENLTDTELLAILIR"
BASE COUNT         1055 a          544 c          714 g          846 t
ORIGIN      
        1 gagggaaatt ccgggacttt ctaaaattcg acaaatagta tttcaaaata tcgatcaggc
       61 aatctgtttg tcttccgaac gaactatacg ttatctggaa gataacggga ataaaattga
      121 tcctatgaca ataaaaactt atgagtttta cggaggaaag ttatgactca gtcaaaaaaa
      181 atggcacgta tcgcctatga tgcattgaat gaaaaaaaag gtgaagatat taaaatcata
      241 gatattgcag aagtatctac tcttggcgat tactttgtaa ttgctaacgg aaacagctca
      301 agtcaggtga cagctcttgt cgacaacgtg gaagaagaaa tgcataaagc aggattctct
      361 cttcgtcaga gagaagggcg cgcatcggga agttggatac tgcttgattt cggagatgtg
      421 atcattcacg tgtttgataa agaaagcaga gatttttata acattgaacg tgcatggaaa
      481 gacggaaaag aaatcacggt aaaagatctc gattaattca gcagatttct gaaaatctta
      541 aaatgaaatc ctaaatacgc agattttctt gacaagtaaa cttggtatga tataattaaa
      601 tcaacaagta aacttggcaa gatgggaggt attattatgg ataaagaatc tgttttacaa
      661 aaaagaaatc gtgcagagca cagttcgctt ctcatcgttt cagccgggtt tttgcttttg
      721 tgcacttgtg aagtgttcgg aattattacc ggcaaactct ggatcggaca aagggaaatt
      781 gtttttggcg gaacactctt tttcatcatg tttttaagtc agtttgcttt gaatttaaag
      841 tattatttga ttgataaaaa gaaatggcgg attgccgagg ggattttact gatcttttgt
      901 atcggactta cgattttcag aattttgaat cccggacact atcttatgga atttctttaa
      961 ggtgaattat ggaaaatgaa ttagttttaa aaaataactt aaaagaggcg cgtgccaaga
     1021 aacagatttc acaaagagag cttgcagaaa tggtaggagt atcccgcaat acgatcagct
     1081 cgattgaaac aggacagttt aatccgactg caaaactggc gctgattata tgtattgcat
     1141 tggataaaaa atttgaagaa ttattttatt tccgattgtt ttcagataag agtcgatttc
     1201 ttttggaaac cggctctttt tgtaacggtg ataaacagaa gtccgggtga cggatccgca
     1261 acttcgatga ccgcttacaa tcccggaatg cgcctttgaa cacttccggt gacgtattga
     1321 tacaattaat catatagcaa taatggaggt aaaagatgct aagaatcagt cagctaaagc
     1381 tccctgtgga gcatacagaa gcacaattaa aaaagaaact tctgaaaaca gctcatataa
     1441 aagagcagga tctgaagcag ttttttatta gaaaacgatc tgttgacggc cggaaaaaac
     1501 cggaacttta ctacgtatac acagttgatc ttatcgttgc aaatgaagag catgtaaaaa
     1561 aaatttccaa agggaaatta caaactgttc aagaaaaaaa ctatcatatt cccgaacacg
     1621 gcgccaaaaa gctccggaaa agacctgttg ttataggaag tggaccggcg ggcctgtttt
     1681 gcgcgtattt acttgccttt gaaggatacg aacctcttgt gatcgagcgg ggcgcgcctg
     1741 tgagagaacg ccaaaaggtt gtagaacaat tttggaaaga cggcatatta aatactgcct
     1801 caaacgtaca gtttggagag ggtggtgcag gcactttttc agacgggaaa ttaaatactc
     1861 ttgtcaaaga taaattcgga cgaaatcgtt tcgtactaga aacatttgta aaattcggag
     1921 ccccggaaga aattttatgg gaaagcaagc ctcatatagg aacagatatt ttaatcgaag
     1981 tggtagaaag aatgcgagaa gaaattatcc gactcggtgg ggaattcctt tttcattcgc
     2041 aggtaacaga tatccttcct aaagaaaact gtctgatcgt aaacggatgc catcggatag
     2101 aaaccgacgc tgctgtcttt gccgtcggac acagtgcaag agatacattt caaatgcttt
     2161 ttgaccggaa tgttgcaatg aaatctaaat catttgccat aggtgtccgc gcggaacaca
     2221 gacaggatat gatagatgag gcgatgtatg gcagaaaaga aagaggcccg cttggtgccg
     2281 cagcatataa actgacagca cagcttgaaa acggaagagg agtatacacg ttttgtatgt
     2341 gtcccggcgg atatgttgtc aatgcgtcgt ccgagcaagg acgtctagcc gttaacggca
     2401 tgagttatca tgacagagcc ggagaaaatg caaacagcgc tgtgatcgtt accgtaacaa
     2461 aagacgatta tggatctgat catcctcttg ccgggatgga atttcaaaga cggctcgaaa
     2521 aaaaggcatg ggaagaaggc ggcggcaatg ttccgataca acgatttgcc gatttctgca
     2581 cagggacaat atcagtaaag acaggaaatg ttactccgaa cataaaaggg aaatatgtat
     2641 ttggcaatgt gcgcaatata tttcctccgg aacttgcaga atcgattgaa tcggggattc
     2701 gtgcgatggg gaaaaagatc aaagacttcg atcatgatga cgtcatactt tccggtgtag
     2761 aaagcagaac atcttcacct gtaaaaatat tcagaaaaga agactttact tcttcttttc
     2821 cctggattta cccgtgcggt gaaggagccg gatatgcagg cggcattact tctgcggcta
     2881 tggacggcat gaaagttgcg gaagcgatta taaaaacctt ctctgctccc gatagtgtct
     2941 gaatcttcac agaattttaa ggaacaatct ctatacacat tttttaaatt gtgctaaaat
     3001 atatcgttgt attgtatcat cgcactgatt ataaagtgag aaaatggaaa aaatcaatac
     3061 gatcaaagaa atgtacaaag aagatcgtcc ctatgaaaaa tgtgaaaaat tcggagcaga
     3121 gaatctgaca gacacagaac ttctcgcaat tttgatacg
//
LOCUS       DBMDXF010000209         3150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6902_length_3150_cov_1.718268, whole genome shotgun sequence.
ACCESSION   DBMDXF010000209 DBMDXF010000000
VERSION     DBMDXF010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6902_length_3150_cov_1.718268"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="ACZPD4_07695"
     CDS             <1..228
                     /locus_tag="ACZPD4_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR1378971.1"
                     /translation="VEGIAFTVFLGIGFVIGFVLLIIRRKEWISWLKENAAPGSYYLN
                     ALTTPSAVIFIGYNLLNGLMLLTLIFMEPFL"
     assembly_gap    362..371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <372..1967
                     /locus_tag="ACZPD4_07700"
     CDS             <372..1967
                     /locus_tag="ACZPD4_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1378972.1"
                     /translation="HCINCGRCLEVCPQNAKTFASDLERVKGYLAQGFKTIISIAPSY
                     AGVLDFDQPGQVVDALLKLGFYEVRETAEGAALVTNEYKKLVRENEMPNIITTCCPSV
                     NDLIEKYYPDCAKYMAPVVSPMVAHGRYIKKIYGSDVKVVFLGPCIAKKQEAIGDERV
                     FGAVDAILTFEELADWFAEEGIDLRQCEEKPMGNPDPKINRLYPVSGGVIQSVITEED
                     ADGYHKVFVDGLENCMEMLECLGKGELDHCFIEANVCEGGCAKGPASAHWNTSYVKTK
                     VKIENGVSYKAARDLPDMTAEELHKEFKDCSLSDAMPSEEQIRHILKSTGKYCAEDEL
                     NCGACGYSTCREKAVAVFQGKAELSMCMPYALTQAESMSNVVMDVTPNMIFVIGSDMK
                     ILDCNRKGQDLLGVGREEAVQRYIFEFIETEDIEEVLDTKKPVMHKKIKIDNDRLTVE
                     ETIVYIDNLDAALVTYQDVTREEKVREQHYKLKVGTVEMAQKVIEKQMMVAQEIAGLL
                     GETTAETKVTLTKLRDSILNEED"
     gene            2089..>3150
                     /locus_tag="ACZPD4_07705"
     CDS             2089..>3150
                     /locus_tag="ACZPD4_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1378973.1"
                     /translation="MSVSIDVAWKSLNKHHEELCGDKVEVLKTDDSDILILADGMGSG
                     VKANILATLTSKILGTMLYEGAALESCVETIAKTLPICQVRKVAYATFSVLQIFHSGE
                     AYLVEFDNPSCVFIRDGKIVNYAYETREIEGKKIHEYRFQVQKNDCFVLMSDGVIYAG
                     AGSILNLQGWTWEAMAEYTLKCTKKTMSASRLAVMLSQACDELYEEKPGDDTTVAVAR
                     VIDRRIVNVFTGPPKEKKDDERMMYEFMHTEGKKVVAGGTSANIAARILGKEIVTKVD
                     SRNPDIPPMAVIEGIDLVTEGVLTLGKCIKLLKKYVRDEFDAEFFDELDAENGASKLA
                     KLLIEECTELNLFVGTAVND"
BASE COUNT          953 a          507 c          915 g          765 t
ORIGIN      
        1 gtggaaggga ttgcgtttac tgtattttta ggtatcggtt ttgtgatcgg tttcgttctt
       61 ctgattataa gaagaaaaga gtggattagc tggctgaaag aaaacgctgc gcccgggagt
      121 tattatctta atgctcttac aacgccttcg gcagtgatct ttatcggtta taatctgttg
      181 aacgggctga tgcttttgac gctgatattt atggaaccgt ttttgtgaaa aagggaacgt
      241 cctacaggag gtatgaggaa tgaaagtcat tgattttaaa gatgcaaaat gccgtcattg
      301 ttataaatgc gtacgaaact gcgcggtaaa agcgatcagt gtcaaagacg agcaggcgcg
      361 cnnnnnnnnn ncattgtatc aactgcgggc gctgtctgga agtctgtccg cagaacgcaa
      421 aaacatttgc aagcgatctg gaaagagtga aaggatattt ggcacaaggg tttaaaacga
      481 tcatatcgat cgctccgtcc tatgcgggag ttcttgattt tgatcagccg gggcaggtcg
      541 tggacgcgct tttgaaactt ggattttatg aggtgagaga gactgcggaa ggggcggctc
      601 ttgtgacaaa tgaatataaa aaacttgtgc gggaaaatga gatgccgaat attattacga
      661 catgctgtcc gagtgtgaac gatctgatcg aaaaatatta tcccgactgc gcgaagtata
      721 tggctcctgt cgtatcgccg atggtagccc acggcagata tattaagaaa atatacggat
      781 cggatgtgaa agttgtattt ttaggtccgt gtatcgcgaa gaagcaggag gcgatcggag
      841 acgagagagt attcggcgcg gtcgatgcga tcctgacatt tgaagagctg gcagattggt
      901 tcgccgaaga agggattgat cttcgtcaat gcgaagagaa accgatgggg aaccctgatc
      961 ctaagatcaa cagactctat cctgtcagcg gaggggtgat ccagtctgtg atcacagagg
     1021 aagatgcaga cggatatcat aaagtatttg tagacggact ggaaaactgt atggagatgt
     1081 tggaatgtct aggaaaggga gaactggatc attgctttat cgaggcgaac gtctgtgaag
     1141 gcggatgtgc gaaaggacct gcgtcggcac attggaacac atcttatgta aagacaaaag
     1201 taaaaataga aaacggagtt tcttataagg cggcgcgtga tcttccggat atgacggcgg
     1261 aagaactgca caaagagttt aaagactgta gtctttcaga tgcgatgccg tccgaagaac
     1321 agatccgtca tattttgaaa tctaccggaa aatattgtgc ggaagacgaa ctgaactgcg
     1381 gggcatgcgg atattctaca tgcagggaaa aagcggtcgc agtctttcag ggaaaagcgg
     1441 aattgtctat gtgtatgcct tatgcattga cacaggcaga gtcgatgtct aatgtcgtga
     1501 tggatgtgac gccgaatatg atctttgtca tcggaagcga tatgaagata ttagactgca
     1561 acaggaaagg gcaggatctt ctcggagtcg gacgggagga agcggtacaa cggtatattt
     1621 ttgaatttat cgagacagag gatattgaag aagtgctgga cacgaaaaaa ccggtgatgc
     1681 ataaaaagat caagattgat aatgacaggc tgacagtgga agagacgatc gtatacattg
     1741 acaatctgga tgccgctttg gtgacatatc aagatgtgac gcgggaagaa aaagtgcggg
     1801 aacagcatta taagctgaaa gtcggaactg tggaaatggc gcagaaagtg atcgaaaagc
     1861 agatgatggt cgcccaggag atcgcggggc tgcttggcga gacaacggca gagacgaaag
     1921 ttacattgac gaaattgaga gactcgatat tgaatgaaga agattaaagg taacagttca
     1981 gccatgataa gcacgtagtg cggttttacg aatggcgcgg ttgaactgtt acgattaaag
     2041 atgaaacaga tgaaagtcag aaaaaagaag taggacggag agaggttcat gagtgtaagt
     2101 attgatgtag cttggaagag tttgaataaa catcacgaag aattgtgcgg agataaagta
     2161 gaagtgttaa agacggatga ttccgatata ctgatccttg cagatgggat gggaagcggt
     2221 gtgaaggcga atattctggc gacattgacg tcgaaaattc tcggaacgat gttatatgag
     2281 ggggcggcgt tggagtcgtg tgtagaaacg attgcaaaaa cgcttccgat atgtcaggtg
     2341 agaaaggtgg cgtatgcgac attttctgtt ttgcagatct ttcacagcgg tgaggcgtat
     2401 cttgtagagt ttgacaatcc gtcctgtgtg tttatccgtg atgggaagat tgtgaactat
     2461 gcatatgaaa cccgcgagat cgagggaaaa aagatacatg aatatcggtt tcaagtacag
     2521 aaaaacgatt gctttgtcct gatgagcgac ggtgtgatct atgccggagc gggatcgatt
     2581 ctgaaccttc agggatggac gtgggaagcg atggcggagt atacgcttaa atgtacgaaa
     2641 aagacgatgt ctgcgtcccg tctggcagtt atgttaagtc aggcatgcga cgaactgtat
     2701 gaagaaaaac cgggagacga tacgacagtt gcggttgcac gcgtgattga cagaagaatt
     2761 gtaaatgttt ttacaggacc gccgaaagag aaaaaagatg acgagcggat gatgtatgag
     2821 tttatgcata cggaaggaaa gaaagttgtg gcaggaggaa cgagcgctaa tattgcggcg
     2881 cgtatcctcg gaaaagagat tgtcacaaag gtggacagcc gtaatcccga tattcctccg
     2941 atggcagtta tagaaggaat cgatcttgtg acggaaggag tgcttactct cggaaaatgc
     3001 ataaagcttt tgaaaaaata tgtaagagat gagttcgatg cagaattttt tgatgagctt
     3061 gatgcggaaa acggagcgtc taagctggca aaacttttga ttgaagaatg tacggaactg
     3121 aatctgttcg taggaacggc tgtgaatgac
//
LOCUS       DBMDXF010000210         3134 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_6951_length_3134_cov_2.253544, whole genome shotgun sequence.
ACCESSION   DBMDXF010000210 DBMDXF010000000
VERSION     DBMDXF010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_3134_cov_2.253544"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2782
                     /locus_tag="ACZPD4_07710"
     CDS             <1..2782
                     /locus_tag="ACZPD4_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1378974.1"
                     /translation="EIRKDGEDTWTSVTGENGLDLSDKIVNKNDQTLFPAEVTFEAQE
                     ARYVRISGTSSYHWQATDENKYITVGDLAIYGEKVEAKNIAKDANVTAKWTADDTDAA
                     KGGDRPMSMAVDGNKTDFGSNYAEFGADNRRESSYMQVDLGAVCDVNSLSLYRYWGDG
                     RTYGDTVVAVAEKETDFAEGKATIVYNADDQNVHKLYTQAPEKFDEDYAETAQGKSWT
                     LPEGTKAQFVRVYMYGRANNDTTTNHVVELEVYGTKPEEGETPGVDITALIERLSVLS
                     AVDTSNATTDSAAAFNALLKEGYDLVATGAQTQEEVAAMIKKLEGAEAKLVDASALKK
                     AIADAEKKVETSTVTSAEPVKAKIAEAKQLLVNGTKDAIDAMVAELTEAVKGLVARGD
                     VTDLKALIDQYAKENLKAEDHTTSTWSAYETALNAADAIVKDNSDIDQAAVDAAKKAL
                     EDAHAALAKRGNTDALKALIEEYKELKEADYTHETWVKYEEALEAANGIVADNSNKTQ
                     AEVDTAKEALKTAKEALVKAPVDPQLDKSKLQAAVDAAKEKDENAYTTASYEAMEKVL
                     AEAEDLLANGKDQAAIDAKANELNAAVEALVERGNTDALKALIAQYAAEDLKEADYTV
                     DSWKNYADALKAAEDVVKDNSNLDQSAVDAAKKALEDAHTALVKVEQINKEALKAAID
                     AAKAADANLYTTDSYKAMKTVLSDAEKVLKDSKDQTEIDAAAKALNDAVTALVQRGNT
                     DALKALIEEYKDLKEADYTADSWKEYADALKAAKAIVEDNSNSDQAAVDAALNALRDA
                     RVALKLSGKPSVDKSELQAAYDKYKDKKNDGYTAESWAKFENALKSAKAILDNEAATA
                     DQVKAALAQLNSAAEGLTKTQTPPKNDPQTPSTPSQGGSVQTGDTAHVALWLVLAGMS
                     VIAYVAVRRKRA"
BASE COUNT         1135 a          554 c          802 g          643 t
ORIGIN      
        1 agaaattaga aaagacggag aggatacatg gacttcggta acaggagaaa acgggcttga
       61 tctttcggac aagattgtga ataagaatga tcagacactt ttcccggcag aagttacatt
      121 tgaggcacag gaagctagat atgtacgtat cagtggaacg tcttcttatc actggcaagc
      181 aactgatgaa aataagtata ttacagtcgg tgatcttgcg atctatggag agaaagtaga
      241 agcaaagaac attgcaaagg atgcaaatgt aacggcaaaa tggacggcag atgacacaga
      301 tgcagcaaaa ggcggcgaca gaccgatgtc tatggctgtt gacggaaaca aaactgattt
      361 tggttcaaac tatgccgagt tcggtgcgga caatagacgt gaatcatctt atatgcaggt
      421 tgatttaggc gctgtatgtg atgtgaactc tctgtcactg tatcgttact ggggcgatgg
      481 tagaacatat ggcgatacag tagtagctgt agcagaaaaa gaaacagact ttgcagaagg
      541 aaaagctaca atcgtctata atgcggatga tcagaatgtt cataaactgt atacacaagc
      601 accggaaaaa ttcgatgaag actatgcaga aactgcacag ggtaaatcat ggacgcttcc
      661 ggaaggaacg aaagcacagt ttgttcgcgt ttacatgtac ggaagagcaa acaatgacac
      721 aacaacaaac cacgttgtag agcttgaagt atacggaaca aaaccggaag aaggcgagac
      781 accgggcgta gacattacag ccttgatcga aagattgagc gtattgtcag cggtagatac
      841 gtctaacgca acaacagata gcgcagcagc gtttaatgct cttcttaaag aaggatatga
      901 tcttgtggcg acaggagcac agacacagga agaagtagct gctatgatta agaaactgga
      961 aggcgcagaa gctaaacttg tagatgcatc cgctctgaag aaagcaatcg cagacgcaga
     1021 gaagaaagtt gaaacaagta cagtaacaag tgcagagccg gtaaaagcta aaattgcaga
     1081 agcaaaacag cttcttgtaa acggtacaaa agatgcgatc gacgcaatgg ttgcagaatt
     1141 aactgaagct gtgaaaggac ttgttgctcg cggagatgta acagatctga aagctttgat
     1201 cgatcagtac gcaaaagaga atttgaaagc agaggatcat acaacatcta catggagcgc
     1261 ttatgagact gctctgaatg cggcagacgc aatcgtgaaa gataacagcg acatcgatca
     1321 ggcggcggta gacgcagcga agaaagcgtt agaagatgca catgcagcat tggcaaaacg
     1381 cggaaacaca gatgcattga aagctcttat cgaagagtac aaagaactga aagaagcgga
     1441 ttacacacat gagacatggg tgaaatacga agaagctctt gaagctgcaa atggaatcgt
     1501 ggcagacaac agcaataaga cacaggcaga agtagatacg gcgaaagaag cgttgaagac
     1561 agcaaaagaa gctcttgtaa aagctccggt agatccgcag cttgataaga gcaaactgca
     1621 ggcagcagtt gatgcggcaa aagaaaaaga cgaaaacgca tatacaacag cttcttacga
     1681 agcaatggaa aaagttcttg cagaagcaga agacctcctg gcaaacggta aggatcaggc
     1741 ggcaatcgat gcaaaagcaa atgaattgaa cgcggcagtt gaagctcttg tagaacgtgg
     1801 aaacacagat gcgttgaaag ctttgattgc tcagtatgcg gcagaagatc tgaaagaagc
     1861 agactacaca gttgattctt ggaagaatta tgcagatgca ttgaaagcgg cagaggatgt
     1921 tgtgaaagac aacagcaatc ttgatcagtc agcggtagac gcagcgaaga aagcgttaga
     1981 agatgctcat acagcattag taaaagtaga gcagatcaat aaagaggcgt taaaagcagc
     2041 aatcgatgca gcgaaagcgg ctgatgcgaa cttgtacaca acagattctt acaaagcaat
     2101 gaaaacagtt ctttcagatg cagaaaaggt actgaaagac agtaaagatc agacagagat
     2161 cgatgcagca gcaaaagcgt tgaacgatgc ggttacagcg cttgtacagc gtggaaacac
     2221 agatgcattg aaagctttga tcgaagagta taaagatctg aaagaggcag actatacagc
     2281 agattcttgg aaagaatatg cagatgcgct gaaagcggca aaagcaatcg tggaagacaa
     2341 cagcaacagt gatcaggcag cggtagacgc agcgcttaat gcgttaagag acgctcgcgt
     2401 agcattgaag ctttccggaa aaccgtctgt tgataagagc gagcttcagg cagcttacga
     2461 caagtacaaa gataagaaga acgacggtta cacagcagag agctgggcga agtttgaaaa
     2521 cgctctgaaa tctgctaagg caatcttgga taacgaggct gcaacagcag atcaggtaaa
     2581 agctgctttg gcacagttga attcagcggc agaaggtttg acgaagactc agactccgcc
     2641 gaagaatgat ccgcagactc cgtcaactcc gtcacagggc ggaagcgtac agacaggaga
     2701 tacggcacat gtagctctgt ggcttgtact tgcgggaatg tcggtaattg cttatgtagc
     2761 ggttagaaga aaaagagcat agtgaaaact tgtcttttga ttaaggcagt aaacatttaa
     2821 gcttttatgg aatagggcgt tcggtaacgg acgccttatt cttttttgca gagacagcaa
     2881 tattgtcaga aaattcatac aatataaaag taaaatgtaa ttatttaaag ttttttataa
     2941 aaatgtattg acaaaataaa actcaggtga tattataaag acatccaaaa gagatacaac
     3001 aaaataaaaa caaaagcatc tcaaaaggat atttaactaa gaaggcatcg tatcagttgc
     3061 ccgattagat taaaaataaa cggatagcag gagggaaaga ccaatggaca gcgtgatttc
     3121 agtgaatcat caat
//
LOCUS       DBMDXF010000211         3092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7058_length_3092_cov_1.677031, whole genome shotgun sequence.
ACCESSION   DBMDXF010000211 DBMDXF010000000
VERSION     DBMDXF010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_3092_cov_1.677031"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3092)
                     /locus_tag="ACZPD4_07715"
     CDS             complement(<1..>3092)
                     /locus_tag="ACZPD4_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1378975.1"
                     /translation="NKTPGAKWPFYGTIDSVNVFDETLNAEQVMELHRRTLPQDEPEY
                     GENVYKTGEYGIYDMGDYDSYNYRIPAMVTTSKGTLIAAADQRNTHWSDWGNIDTVVR
                     RSTDNGLTWEEPIDVIDLKSQSYFNGTQSAYTIDPALIAEGENGKNPGRVWMLVDMMP
                     ESTNGSQGTYSIKETGTGYVKVDGKDYLALYDKDNNQYTLRENGEVFDKENRKTDYVV
                     KQFEGNDKQGYHEKGDLYQSGKYVGNIYLRSASKNNDSAPLHPKQTCYLWLSYSDDDG
                     MTWSEPVDITPQVKEDWMRFCGVGPGFGVQLRYDEKHPGRLIFPIYYTIAGSGIGFQS
                     SACVYSDDGGKTWHRGESPNDGRINKDGQETSSQNPVGISELTESQIIELSSGNLLQF
                     MRNTRGNGKVVVSRSTDGGATWSDPIDTTAPEVYCQLSVLYYDKNGTGGKDRVIMSNP
                     GGTGRNNGTLRIGEVTETKDSFSVDWVEEKMFCPNNYAYSCLTKMKDGNMGLLYEHQN
                     TIKFTAFNLEYIKDEVNLLSPTITAVTYKVEKTDDHAYTLPGDKYVITVKTDQNVTVQ
                     GTPKFRFMLNGKGRYANYVSGGNDDKELVFEYTVQKGDEGTIQFKGPKIICDETGTVK
                     NANDLCVSSGDMDVKMGYIGVDPSDAARDIATDQMSAAAGSAQSGQEASNVLDGKADT
                     LWHTKWSAGHGREKHWIQFELKDFYLIDGIRYQPRPSGGTNGIITEYKVEVSNDGVHF
                     EQAATGTWDANAAWKLASFEPVCAKYVRLTSLDAISVEADNDYASAAEIRLTGTKTEK
                     PPVAEADKKSLGAAIAYTKDVKTKPEYQYVVPIVKQKLDEALEAAETVFANEAATQGE
                     VDKAEADLKKMLEYLSFTGDAKLLRELVDIAKEINTDIYTEQSANAFKEALEEAEAVL
                     ANENALQDELDAAHKKLQEAMDGLKVIPVDKSQLRKLVDDSRKYVEKLNEYTAQSGQI
                     FMGAFEAAENILEKQDATKEEVDQACANLRNAVFGLRLIPSKEKLEEATHAKLVFFTN
                     "
BASE COUNT          712 a          811 c          525 g         1044 t
ORIGIN      
        1 tatttgtgaa gaaaaccagc ttggcatgag tcgcttcttc cagtttttct ttgctcggta
       61 tcaacctcaa tccaaagact gcattgcgaa gatttgcaca cgcctgatcc acttcctctt
      121 ttgtcgcgtc ctgtttttct aaaatatttt cagccgcctc aaatgctccc ataaaaatct
      181 gaccgctttg ggctgtatac tcattcagtt tttctacata tttccgcgaa tcgtctacaa
      241 gtttccgaag ctgtgattta tccacaggta tcactttcaa tccatccatt gcctcttgta
      301 attttttatg agccgcgtca agttcatcct gcagcgcatt ttcattggca agcacagctt
      361 ccgcttcttc aagcgcttct ttaaacgcat tcgcactctg ctctgtataa atatctgtat
      421 tgatttcttt tgcaatatct accagctctc tgagcaattt tgcatctcct gtaaaagaca
      481 aatattcaag cattttttta agatcagctt ctgccttgtc aacttctccc tgtgttgcgg
      541 cttcatttgc aaaaacagtt tcagccgctt caagcgcctc gtctaatttc tgttttacga
      601 tcggcacaac atattggtat tcaggctttg tttttacatc tttcgtatac gcaatcgcag
      661 ctcccagaga cttcttatcc gcttcggcaa ccggaggttt ctctgttttt gttcctgtaa
      721 ggcggatttc cgctgccgaa gcataatcat tatccgcctc cacactgatt gcatccaaag
      781 atgtcaaacg cacatatttt gcacataccg gctcaaatga agccagtttc catgcagcgt
      841 tcgcgtccca tgttcctgtt gccgcctgtt caaagtgaac gccgtcatta cttacttcca
      901 ctttgtattc cgtaataata ccgtttgttc ctcccgaagg ccgcggctga taacggattc
      961 cgtcgatcaa ataaaagtct ttcagttcaa actgaatcca atgtttttcg cgtccgtgac
     1021 cggcgctcca ttttgtatgc cacaatgtat ccgcttttcc gtccagaaca ttcgacgcct
     1081 cctgacccga ttgagccgaa cctgccgcag cgctcatctg atctgtcgca atatctcttg
     1141 ccgcatccga aggatctact ccgatgtatc ccattttaac atccatatct cccgaactta
     1201 cgcacaaatc atttgcattt tttactgtgc ctgtttcatc gcagataatt tttggtcctt
     1261 taaactgaat agttccttca tcgccttttt gtacagtgta ttcaaatact aactccttgt
     1321 cgtcattccc accggataca tagtttgcat aacggccttt accatttaac ataaatctga
     1381 atttcggtgt tccttgcaca gtgacattct gatctgtttt cactgtgatc acatatttat
     1441 ctcccgggag cgtataagcg tgatcgtctg ttttttccac tttgtatgta acagctgtaa
     1501 tcgtcggact caataaattc acttcatctt taatatactc taaattaaac gcagtaaatt
     1561 tgatcgtatt ctgatgttca tagagaagtc ccatatttcc gtctttcatt tttgtcagac
     1621 aagaatatgc gtaattatta ggacagaaca ttttttcttc tacccagtct acagaaaaac
     1681 tatcctttgt ttctgtcact tctccgattc gcaatgttcc gttatttctt cctgtccctc
     1741 ccggattcga catgatcact ctgtctttgc cgcctgttcc gtttttatca taatataaaa
     1801 cagataactg acagtatact tccggcgccg ttgtatcgat cggatcactc catgtcgctc
     1861 ctccgtctgt acttcttgaa acaacaactt ttccgtttcc gcgtgtattt ctcataaact
     1921 gaagcaaatt tccgctgctt agttcaatga tctgactttc tgtcagttca ctgatgccga
     1981 ccggattttg cgaagaagtc tcctgtccgt ctttgttgat tcttccgtca ttcggagatt
     2041 ctcctctatg ccatgttttt ccgccgtcat cactgtaaac acaagccgaa gactggaatc
     2101 ctataccgct tccggcaatc gtataataaa tcgggaatat aagtcttccc ggatgctttt
     2161 catcataccg aagctgaact ccgaatccag gaccgacacc gcaaaatctc atccagtctt
     2221 cttttacctg cggcgtaatg tctaccggtt cgctccacgt cataccgtcg tcatcgctat
     2281 aacttagcca caaatagcat gtctgcttag gatgaagcgg agcggaatcg ttgttcttag
     2341 aagcgcttct cagataaata tttcccacat atttaccgga ttgatacaga tcgccttttt
     2401 cgtggtatcc ttgtttatca tttccttcaa actgttttac cacataatcg gttttgcgat
     2461 tctccttatc aaatacttct ccgttctctc gaagcgtata ttgattattg tctttatcat
     2521 aaagcgccag ataatctttt ccatctactt tcacatagcc tgttcccgtt tctttgatcg
     2581 aatatgtacc ctggcttcca ttcgtcgatt ccggcatcat atctacaagc atccagacac
     2641 gccccggatt ctttccgttt tcgccctctg cgatcaatgc cgggtcaatc gtatacgcag
     2701 attgtgtgcc gttaaaatag gattgtgatt tcagatcgat tacatcgatc ggctcttccc
     2761 atgtcagtcc gttatctgta cttcttctga caactgtatc aatatttccc cagtcggacc
     2821 aatgtgtatt tctttgatcg gctgctgcga tcagagttcc tttgctcgtt gtaaccattg
     2881 cgggaatacg gtaattataa gaatcataat cgcccatatc ataaataccg tactcgcctg
     2941 tcttatagac gttctctccg tattccggtt catcctgcgg caaagtacgt ctatgaagct
     3001 ccataacctg ttccgcattt aacgtttcat caaacacatt gacgctgtcg atcgttccgt
     3061 agaatggcca tttcgcgccc ggcgtcttat tc
//
LOCUS       DBMDXF010000212         3062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7150_length_3062_cov_1.345154, whole genome shotgun sequence.
ACCESSION   DBMDXF010000212 DBMDXF010000000
VERSION     DBMDXF010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7150_length_3062_cov_1.345154"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="ACZPD4_07720"
     CDS             <1..170
                     /locus_tag="ACZPD4_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR1378976.1"
                     /translation="IVLNPDNLHANEHGVEAAKKGKDIADPWYTGDFDTTYRDVKKGC
                     EALLNDCLLRF"
     gene            complement(159..869)
                     /locus_tag="ACZPD4_07725"
     CDS             complement(159..869)
                     /locus_tag="ACZPD4_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR1378977.1"
                     /translation="MKSHTYYDILGVSRDATLDEITTAKNALAKVYHPDANAHKNIDT
                     TAFMQEILEAYRVLSNPEKRRKYNQETFGETERVFKTFTLTPENEEENTGSFVTYWNM
                     SNQLRTILNKSIRLMKQETQKKTLTQRVFQKWGKYQKEETIRNQQIAKLSTQAVQYIT
                     ALKMAGIPMDYWSSDAMNWILVRWGQKQSVDYHTLFSRYDDYVEETLSNSEKIRLKNQ
                     NKRFHHNLKKLLSYALKA"
     gene            1112..2416
                     /locus_tag="ACZPD4_07730"
     CDS             1112..2416
                     /locus_tag="ACZPD4_07730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1378978.1"
                     /translation="MRNARTVIAGVLCALMICCMLGNAYTVKGKEMLLAPENKETKDQ
                     NTENSKIEDNNTENEEELNKGAKKEDTSMQIGAPSALLMEASTGTVLFEQDADTKRPP
                     ASVTKIMTMLLIFDAVESKKISLEDKVHVSEFAASMGGSQVFLETGETQTVDTMLKCI
                     SIASANDACVAMAEYISGSEEAFVQEMNKRAKALGMHNTNFVNCNGLDAQGHVTTARD
                     IALMSRELITKYPQIHEYAMTWMENITHETKKGTSEFGLTNTNKLVRQYEYATGLKTG
                     STGEAKFCVSATGKKDDVELIAVVMAADTSKERFADAVKMMNYGFGKCQVYSDEKPMK
                     TKYAVVKNGVEENIEVERGEKFTYLDVKGKDMKKIKKIEKINKKILAPVRKGDVVGSI
                     VYTLDGNEIGRSDIIAKRGVQKAGFSDYLIKVSAKFLIGEKN"
     gene            2570..>3062
                     /locus_tag="ACZPD4_07735"
     CDS             2570..>3062
                     /locus_tag="ACZPD4_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1378979.1"
                     /translation="MNNTAWWIIIFILILLILAAGEIYRETHTFRVRKYKVKTKKNIG
                     IQNCVKVIFLSDLHNCVYGNKNDKLYKAIQAEMPDMILIGGDMLVAKEGSSVQEALEF
                     VKKLPHICQVYYTNGNHEQRMKENTDIYGDTYERYKAKLENCGVCFLENKAYSTIFQL
                     CFVS"
BASE COUNT         1032 a          437 c          695 g          898 t
ORIGIN      
        1 agatcgtatt gaatcctgat aatcttcatg ccaatgaaca tggtgtagaa gcagcgaaaa
       61 aaggaaaaga cattgcagat ccgtggtata caggtgactt tgatacgact tacagagatg
      121 taaaaaaggg ttgcgaggct cttttgaatg actgcctctt acgcttttaa agcgtaggag
      181 agcagttttt ttaaattgtg atgaaaacgt ttgttttgat tttttaatcg aattttttcg
      241 gaatttgaca atgtttcttc tacataatca tcatagcgtg aaaagagagt atgataatcg
      301 acagattgtt tttgccccca acgtacaagt atccaattca ttgcatcgga actccagtaa
      361 tccatcggaa ttccagccat tttaagagcg gtaatatatt ggacggcctg tgtggataat
      421 tttgcgatct gctgattgcg gatcgtttct tctttttgat atttgcccca tttctgaaaa
      481 acgcgttggg tcagtgtttt cttttgtgtt tcctgcttca tcaaccgaat gcttttattg
      541 agtatagttc taagctgatt agacatattc caatatgtga caaaagaacc tgtattttct
      601 tcttcgtttt ccggagttaa tgtaaatgtt ttgaaaacgc gttcggtttc gccgaatgtt
      661 tcttggttat atttgcggcg cttttccgga ttcgataaaa cccggtatgc ctcaagtatc
      721 tcctgcataa aagcggttgt gtcaatattt ttatgtgcat ttgcatctgg atgatatact
      781 ttcgccagtg catttttagc agttgtgatc tcgtctaaag tcgcatctct tgaaacacct
      841 aaaatatcat aataagtatg agacttcatt agtgacctcc ttattaattt tcccgttgat
      901 tctcccttgc ttaatatact ctacggattg ataaaagtca atgaaacagg taaaaatgag
      961 caagatttga gaggctttta taggatagtt atttttagtc agcgcagtta tatgctgaaa
     1021 tctatcttga ggaaaaagag agattaagta atactttttg caggatttgc ttatatttat
     1081 aatagatgaa attttggaga gtggatcaaa aatgagaaat gcaagaacgg taatcgcagg
     1141 tgttttatgt gcattgatga tctgttgcat gttgggaaac gcatatacgg taaaaggcaa
     1201 ggaaatgtta ttagcgccgg aaaataaaga aacaaaagat caaaatacag aaaacagcaa
     1261 gatagaagat aacaatacag agaatgaaga agaactaaat aaaggcgcaa aaaaggagga
     1321 tacaagtatg caaatcggtg ctccttcagc tcttttgatg gaagcatcta ccggaacggt
     1381 attgtttgaa caggatgcgg atacgaaacg tccacctgca agtgttacga aaattatgac
     1441 aatgcttctt atttttgatg ctgttgaaag taaaaaaatt tctttggaag ataaagttca
     1501 cgtatcggaa tttgcagcgt ctatgggcgg ctctcaggtg tttttagaga caggggaaac
     1561 tcagacggtc gatacaatgc taaagtgcat ttcaatcgca agtgcaaacg atgcttgtgt
     1621 ggcaatggca gagtatataa gcggcagtga agaggctttt gtacaagaaa tgaacaagcg
     1681 ggcaaaagca ttggggatgc ataatacaaa ttttgtaaat tgcaacggtt tagatgcaca
     1741 ggggcatgtg acgactgcaa gagatattgc gctgatgtcg cgggaattaa ttacgaaata
     1801 tcctcagatt catgagtatg cgatgacatg gatggaaaat attactcatg aaacgaagaa
     1861 aggtacttcg gaatttggac ttacaaatac aaataaactt gtaaggcagt atgaatatgc
     1921 taccggtttg aaaacagggt cgacaggaga agcaaaattt tgcgtttcgg caacgggaaa
     1981 gaaggatgat gtggagttga tcgcggttgt tatggctgcc gatacatcga aggagagatt
     2041 tgcggatgcg gttaaaatga tgaattacgg ttttggaaaa tgtcaggtgt attcggacga
     2101 gaaaccgatg aaaacaaaat atgcagttgt aaaaaatgga gtcgaagaaa atatagaagt
     2161 agagcgcgga gaaaagttta cttatctaga tgtaaaagga aaggatatga agaaaattaa
     2221 aaaaattgag aaaataaata aaaagatatt agcgccggtt cgaaaaggag atgttgtagg
     2281 ctcgatcgtt tatactctgg atggaaatga aatcgggcgc agcgatatca tagcaaaaag
     2341 aggtgtacaa aaagcaggat tttcggatta tttaataaag gtatcggcaa aatttttgat
     2401 tggagaaaag aattaataaa agctgcttta gatctgcgga ataatttcac acaaaaagtt
     2461 gtttacgtgc tgatgtgaag aaatacttga gtttcagaat accgctgttt tataatgaga
     2521 gaaaatgagg aaagtttttt ataggaatat cgttaaagga gttttataaa tgaataatac
     2581 agcatggtgg ataattattt ttatccttat tttgctcatt ttagcggcag gagaaattta
     2641 tcgggaaact catacattcc gggtgagaaa atataaagta aaaacgaaaa aaaacatcgg
     2701 cattcaaaac tgtgtaaaag tgattttttt aagtgacctt cataactgtg tatacggaaa
     2761 taaaaacgat aaattgtata aggcgattca ggcagaaatg ccggatatga ttttaatcgg
     2821 aggggatatg ctggtcgcaa aagaaggcag ttccgtacag gaagctcttg agtttgtgaa
     2881 gaaattaccg catatttgtc aggtgtatta cacaaatggc aaccatgaac agagaatgaa
     2941 agaaaataca gatatatacg gggatacata cgaacgatac aaagcaaagc tggaaaattg
     3001 tggagtatgc tttttggaga ataaagcata ctccacaatt ttccagcttt gctttgtatc
     3061 gt
//
LOCUS       DBMDXF010000213         3039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7225_length_3039_cov_1.775017, whole genome shotgun sequence.
ACCESSION   DBMDXF010000213 DBMDXF010000000
VERSION     DBMDXF010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7225_length_3039_cov_1.775017"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="ACZPD4_07740"
     CDS             <1..576
                     /locus_tag="ACZPD4_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1378980.1"
                     /translation="EMDTGKVQVKIEFAESYYYEVISEVTGEEIHGEYELMAVMKELS
                     AMREEFSRIKDAFADVKMKGYGVVSPSKEDIRLEEPVIIKQGSKFGVKIRSEAPSIHM
                     IRANIETEIAPIVGNEKQAEDLAGYIKKESETPEGVWGTNIFGKTVEELVMDGMRNKI
                     AMINDESQVKLQDSMQKIVNDSNGGMVCIII"
     gene            651..1493
                     /locus_tag="ACZPD4_07745"
     CDS             651..1493
                     /locus_tag="ACZPD4_07745"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1378981.1"
                     /translation="MSTQFDKLEKCYEYCKTVTDFIPRVGLILGSGLGGYAKNMKVVK
                     EISYDQIPGFPVSTVQGHDGKFLLGYIGKVPAMVMKGRVHFYEGYTPQEVVLPVRLMK
                     RMGIEILFLTNASGGINRDFQVGDFMMITDHISSFVPSPLIGENVSELGERFPDMTKI
                     YDANLNQIIEKTAAEEKIILKKGVYLQTSGPNFESPAEVRMYGILGADAVGMSTACEA
                     MAARHAGIRVCGISCVSNMASGLSGEELSHLDVQAVADRRAQEFERLVTRSIEKMCED
                     ERSE"
     gene            1504..2823
                     /locus_tag="ACZPD4_07750"
     CDS             1504..2823
                     /locus_tag="ACZPD4_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1378982.1"
                     /translation="MRTRIYNARILTMEEKRPVFKGEIYIDGGKIFRIKEAKTEKSER
                     TEKPALNEEEKWDEEIDAEGNLIMPGFKNAHTHSAMTFLRSYADDMKLQEWLFDKVFP
                     AEAKLTSDDVYHLTKLAILEYLTSGITAAFDMYKLKQDSAKAAEELGFRMVFCGDIND
                     FGGTLEEMEQDYFRYQCEETPLLSYQFGFHAEYTTSRNLMEGIAQLAGKYKKPVYVHC
                     SETKREVEECREKTGMTPIAYMDSLGLFENGGGLFHGVHLDEADFDIMKKKKICVVTN
                     PASNLKLASGIAPVKTYLEKGIPVAIGTDGPASNNCLDMFKEMFLVTGLQKVVHNDPE
                     AVPAADVLKMATVNGAYAMGLDNCDILAEGKYADLIMIDLMQPNMQPIQNIEKNVVYS
                     ASKLNVKMTMINGRAVYQNGKFKIGESVEDIYENANRIAERILDGKE"
BASE COUNT         1029 a          467 c          798 g          745 t
ORIGIN      
        1 gagatggata cgggaaaagt acaggtgaaa attgaatttg ccgaatctta ttactatgaa
       61 gtgatcagtg aagtgacagg agaagagatt cacggcgaat atgagcttat ggcagtgatg
      121 aaagagttgt cggcaatgcg ggaagaattt tcccggatca aagatgcttt tgccgatgtg
      181 aaaatgaaag gatacggcgt cgtgagtccg tcaaaagaag atatccggct tgaagagccg
      241 gtgattatca aacagggaag taaattcgga gtaaagattc gttcggaggc gccgtcgatc
      301 catatgatcc gtgcaaatat agagacagag atcgcaccga tcgtcggaaa tgagaagcag
      361 gcggaagatc ttgccggata cattaaaaaa gagagtgaga ctccggaggg cgtatgggga
      421 acaaatattt tcggaaaaac agtagaagag cttgtgatgg acggcatgag aaataaaata
      481 gcgatgatta atgatgaaag ccaggtaaaa cttcaggatt ctatgcagaa gatcgtaaat
      541 gattcaaatg gcggcatggt atgtattatt atctgacggc agttcttgac aagaaaataa
      601 aaatagtcta ggctttcagt atgacaaaag aattcaatag gaggcgtaag atgagtacgc
      661 agtttgacaa acttgagaaa tgctatgaat attgtaaaac agtgacagat tttatccccc
      721 gcgtcggtct gatcttagga tccggactcg ggggatatgc gaaaaatatg aaagttgtaa
      781 aagagatatc atatgaccaa attccggggt tcccggtttc aacagtgcag ggacatgatg
      841 gaaaatttct ccttggttat atagggaaag ttcctgcaat ggtaatgaaa ggacgcgttc
      901 atttttatga aggatatacg cctcaggagg ttgttcttcc ggtgcgtctt atgaagcgta
      961 tggggattga aattcttttt ctgacgaatg catcaggtgg aatcaatcgt gattttcagg
     1021 taggagattt tatgatgatc acagatcata tttcatcttt cgttccgtct cctttgatcg
     1081 gagagaacgt atcggaactt ggcgaaagat ttcccgacat gacaaaaatc tatgatgcca
     1141 atctgaatca gatcattgag aaaactgccg cggaagaaaa gattattttg aaaaaagggg
     1201 tatatcttca gacgtccggt ccgaattttg aaagtccggc agaagtgcgt atgtatggaa
     1261 tattaggtgc agatgcagtc ggaatgagta cggcgtgcga agcgatggca gctcgtcatg
     1321 cgggaatacg ggtgtgcggg atctcatgcg tctctaatat ggcaagcgga ttgtcgggtg
     1381 aagaattaag ccatcttgac gtacaggctg tggcagatcg aagagctcag gagtttgaac
     1441 gtcttgtgac aagatcgatt gaaaagatgt gcgaggatga aagatcagag taggagaaaa
     1501 cggatgagga cgagaatata caatgcaaga attttgacaa tggaagaaaa acgacctgtt
     1561 tttaaaggtg agatctatat tgacggcgga aagattttcc gcataaaaga agccaaaacg
     1621 gaaaaaagtg agcggacaga aaaaccggct ttaaatgaag aagaaaaatg ggacgaagaa
     1681 atcgacgcag agggaaatct gattatgccc gggtttaaaa atgcgcatac tcattctgct
     1741 atgacatttc tgcgttctta tgcagatgat atgaaacttc aggaatggct ttttgataag
     1801 gtatttccgg cagaagcaaa gctgacatcc gatgacgtat atcatttgac aaaacttgcg
     1861 attttggaat atttgacaag cgggattacg gcggcttttg atatgtataa attaaaacaa
     1921 gacagtgcaa aagcggcaga agaactgggc tttagaatgg ttttttgcgg agatatcaat
     1981 gatttcggcg gtactttaga agagatggag caagactatt ttcggtatca atgtgaagaa
     2041 actccgcttt tgtcctatca gttcggattt catgcagaat atacgacaag ccgcaatttg
     2101 atggaaggga tcgcgcagct tgccggaaaa tataaaaagc ctgtttatgt ccattgttcg
     2161 gagacaaaaa gagaagtgga ggaatgtcgg gaaaaaacag gtatgacacc gatcgcatat
     2221 atggattctc tcggcctttt tgaaaacgga ggaggattat tccacggcgt tcatttagat
     2281 gaagcagatt ttgacattat gaaaaagaaa aaaatatgtg tcgttacaaa tccggcttct
     2341 aatttaaaac tggcaagcgg aattgccccg gtcaaaacgt atcttgaaaa aggaattccg
     2401 gttgctatag gaacagacgg accggcaagt aataattgcc ttgatatgtt taaagaaatg
     2461 tttcttgtga cagggcttca aaaagtagtt cataatgatc cggaggctgt tccggcagca
     2521 gacgtactga aaatggcgac tgtcaacgga gcttacgcga tgggacttga caactgtgat
     2581 atacttgcag aaggaaaata cgcagatttg attatgatag atctgatgca gccgaatatg
     2641 cagccgatac aaaatattga aaaaaatgtt gtgtacagcg ccagtaagct gaatgtaaaa
     2701 atgacgatga taaacgggag agctgtttat caaaacggga agtttaaaat cggggaaagc
     2761 gtggaagaca tttatgaaaa tgcaaaccgg atcgcagaga ggatactaga tggcaaagaa
     2821 taaatgctca cagacgtatc ggagatttat tatttgtgca gaaggggaaa caaatgatta
     2881 aaacagatag agagaaacaa atgaaagggg ttacaaagca atgagaacag caatatttac
     2941 gatcgataca gaaagttatg aagcaaagaa aaaaagtgcg gcaggagagg ctttgaagcg
     3001 tatgcttgaa caaaacaggc tggaagtgaa agctgcgaa
//
LOCUS       DBMDXF010000214         3037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7233_length_3037_cov_1.717643, whole genome shotgun sequence.
ACCESSION   DBMDXF010000214 DBMDXF010000000
VERSION     DBMDXF010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7233_length_3037_cov_1.717643"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="ligA"
                     /locus_tag="ACZPD4_07755"
     CDS             complement(<1..1352)
                     /gene="ligA"
                     /locus_tag="ACZPD4_07755"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1378983.1"
                     /translation="MEKKKKTKRMRELVELLNKARRAYEQENTEIMSNFEYDRLYDEL
                     LSLEKELETTLASSPTVNVGYEVLSELPKERHETPMLSLDKTKDVERLREFLGDQQAV
                     ISWKMDGLTIVLTYENGVLKKAVTRGNGEIGEVVTNNAKVFRNVPLSIPFKGELVLRG
                     EAVISYKDFEKINEEIGDADAKYKNPRNLCSGSVRQLNNEVTAKRNVRFYAFTLVKAD
                     NVDFENSRAYQLKWLQEQGFDVVEHHLVTRDTVGDEVAWFAEHIRENEVPSDGLVLIY
                     DDISYGQSLGTTAKFPRDSFAFKWEDEVRKTTIREIEWSPSRTGLINPVAIFDPVELE
                     GTTVSRASVHNISIMEELELGIGDTVTVYKANMIIPQIAENLTRSGVSEIPKECPVCK
                     KPTKISMENNTKTLYCMNPKCQAKHVKSFSLFASRDAMNIEGLSEATLEKFIINGYVK
                     "
     gene            complement(1395..2129)
                     /locus_tag="ACZPD4_07760"
     CDS             complement(1395..2129)
                     /locus_tag="ACZPD4_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1378984.1"
                     /translation="MSNRIREEFLQAKESVRLNKYLSEAGVCSRREADRLIESGKVTV
                     DGVTAQMGMRVTAGQIVKVGKKTVSKQDEMIVLAVNKPKGIVCTEDQRERDSIVRFLN
                     YPVRVTYAGRLDKDSRGLLLMTNNGDIINQMMRAANRPEKEYKVTVDKEITEQFIKKM
                     SEGVPILDTVTRPCTVKKIGKYTFSIILTQGLNRQIRRMCAAFGYEVKDLVRIRIMNI
                     RLGSLKEGAYRKLTDEELEELYEMLK"
     gene            complement(2122..>3037)
                     /locus_tag="ACZPD4_07765"
     CDS             complement(2122..>3037)
                     /locus_tag="ACZPD4_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1378985.1"
                     /translation="KMKVNADKISGTKKENGVFIGKSGQLLEDIAVPDKEQLEANINA
                     VKNFAQTYENIPTTVMLVPDAACILSNRLPAFATVEDQRQMFGMVERNLGDTVNWVDT
                     VSIMNKHKSEKLYYKTDHHWTTQGAFYMFKDTASLFGIKGDVSDDYVSYTVTDDFNGV
                     LASKSGCGLDEKEKIDIYAPTNGDDDVIVNYVNETKKTTSLYDSSKLKTRDKYGVFLG
                     GNTSVIDIKTVSTSQKRLLVVKDSFADCFIPFLTPHYREIVVVDPRYYSGTMQDIMDT
                     YRITDALILYSGNTFFTDNNISGVFTGE"
BASE COUNT          745 a          800 c          507 g          985 t
ORIGIN      
        1 tctttgacat acccgttaat gatgaatttt tccaaagtcg cctcggacag tccttcgata
       61 ttcatagcgt ctctgcttgc aaacaaactg aatgacttca catgctttgc ctgacatttc
      121 ggattcatac aatacagcgt tttcgtatta ttttccatag atatttttgt cggcttttta
      181 cagacaggac attctttcgg aatctcgctt actccgctgc gcgtcagatt ttccgcaatc
      241 tgggggatga tcatatttgc cttatacacc gtaaccgtat ctccgatccc cagttccagt
      301 tcctccataa tactgatatt atgaacgctt gcacggctta ctgttgttcc ttctaattct
      361 acaggatcaa agatcgcaac aggattgatc agtcctgttc tggacggact ccattcaatc
      421 tcacggatcg tcgtctttcg cacttcatcc tcccacttaa acgcaaagga atctcgcgga
      481 aactttgccg ttgttccaag tgactgccca taagaaatat catcatatat aagaacaagt
      541 ccgtccgacg gcacttcgtt ttcccgaata tgctctgcaa accacgcaac ttcatcaccg
      601 acggtatccc gggtaacgag atgatgctct acaacatcga atccttgttc ctgcagccat
      661 ttcagttgat atgctcttga attttcaaaa tccacattgt cagctttcac aagcgtgaac
      721 gcataaaacc ttacatttct cttcgccgtc acttcattgt ttaactggcg gaccgatccg
      781 ctgcacaagt tacggggatt cttgtatttt gcatcggcat ctccgatttc ttcattgatc
      841 ttttcaaaat ctttataaga aataacagct tctccccgaa gcacaagctc ccctttaaac
      901 ggaatagata acggaacatt acgaaatact tttgcgttgt tcgtcacaac ttctccgatc
      961 tccccgtttc cccttgtaac cgcttttttc aaaactccgt tttcataagt cagaacaatc
     1021 gtcagaccat ccatcttcca tgagatcacc gcctgttgat ctcccaaaaa ttccctcaat
     1081 cgttctacat ctttcgtctt atcaagggac aacatcggcg tttcatgacg ctctttcgga
     1141 agctcgctca aaacttcata tcccacattg accgtcgggc ttgaggcaag cgttgtttcc
     1201 agttcttttt ctaaggacaa cagttcgtca taaagacggt catactcgaa attgctcatg
     1261 atctccgtgt tttcctgttc gtaagcacgg cgcgctttat tcagaagttc gacaagttcg
     1321 cgcatccgtt tcgttttctt cttcttttcc atcttcttct ccaacaatta atttctttac
     1381 ggtatcttga ccggtcattt caacatctca tacaattctt ccagttcttc gtccgtcagt
     1441 tttcggtatg ctccttcttt cagactcccg agacgaatat tcataatcct tatacggaca
     1501 agatctttca cctcatatcc gaacgccgca cacatccttc tgatctggcg gttcagcccc
     1561 tgagtcaaga tgatcgaaaa agtatatttc ccgatcttct ttactgtaca tggtcttgtg
     1621 accgtgtcaa ggatcggaac tccttccgac atttttttta taaattgttc cgtgatctct
     1681 ttatcaaccg taactttata ttctttttca gggcggtttg ccgcacgcat catctgattg
     1741 atgatatctc cgttgttcgt cataagcaga agtccgcgcg aatctttgtc cagtctgccc
     1801 gcataagtaa cacgcacagg gtagttcaaa aaacgaacga tactgtcgcg ttctctttga
     1861 tcttcggtac agacgatccc tttcggctta ttcacagcta aaacgatcat ctcatcttgc
     1921 tttgacactg ttttttttcc gactttgaca atctgccccg ccgtaacgcg catccccatc
     1981 tgcgccgtca ctccgtctac cgtcacttta ccgctttcaa taaggcggtc cgcctccctt
     2041 ctggaacaaa cgcccgcctc gcttaagtat ttattcagtc ttaccgactc ttttgcctgt
     2101 aaaaattctt ctctgatccg attactcacc agtaaacact ccacttatat tattgtctgt
     2161 aaaaaatgta tttccgctat acaagatcaa cgcatctgtt atgcggtatg tgtccataat
     2221 atcctgcatc gttccgctgt aatatcttgg atcaacaaca acgatctctc tgtaatgagg
     2281 agttaagaac ggaataaagc agtctgcaaa tgaatctttc accacaagca gtctcttctg
     2341 gctcgtagac actgtcttta tatcgataac tgatgtattc ccgcctaaaa atactccgta
     2401 cttatctctt gtctttagtt tggacgaatc ataaagagaa gttgtctttt tcgtttcatt
     2461 gacataattt acgattacat cgtcatcgcc gtttgtcggc gcgtaaatat cgattttttc
     2521 tttttcatcc agaccgcatc cgctttttga cgcaagaaca ccgttaaaat catctgtcac
     2581 agtataggaa acataatcat cagatacgtc tcctttgatc ccgaacagcg aagccgtatc
     2641 tttgaacata taaaacgctc cctgagtcgt ccagtgatga tccgttttat agtacagctt
     2701 ttctgactta tgtttattca taatagaaac agtatctacc cagttgaccg tatcgcccag
     2761 attacgctct accataccga acatctgacg ctgatcttcc actgtcgcaa atgcaggaag
     2821 acgattgctc aaaatacatg ccgcatccgg aacaagcatt accgtcgtcg gaatgttttc
     2881 atatgtctgt gcaaaatttt tcaccgcatt gatattcgct tcaagctgct ctttatccgg
     2941 aactgcgata tcttctaata gttgtccgct ttttccgatg aacacgccgt tttctttttt
     3001 tgttcccgaa atcttatctg cgtttacttt catcttc
//
LOCUS       DBMDXF010000215         3033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7245_length_3033_cov_2.226808, whole genome shotgun sequence.
ACCESSION   DBMDXF010000215 DBMDXF010000000
VERSION     DBMDXF010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7245_length_3033_cov_2.226808"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="ACZPD4_07770"
     CDS             complement(<1..639)
                     /locus_tag="ACZPD4_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1378986.1"
                     /translation="MPKFSVLIIEDEKNIQTFMGKVLKRHDYQVLYADTGKQGLEMIR
                     SRCPDLILLDLGLPDMDGCSIIRDVRTWASTPIIVISARTAEREKVAALDLGADDYIT
                     KPFGTSELLARIRASLRHSNRLLTDSTFYIRPYRHKDLILDFSKRLLTIRGNAVHLTP
                     IEYKIVAYLAQNSGKVITYASILSNVWGPYADNDNKILRVNMANIRRKIEEDP"
     gene            complement(632..1735)
                     /locus_tag="ACZPD4_07775"
     CDS             complement(632..1735)
                     /locus_tag="ACZPD4_07775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1378987.1"
                     /translation="MNRQNNKTERKKTISDTLFTAFMLFTATAVSFCFFHLGNKNSAN
                     ITVIYTLALILTALKTSGYVYGIVASLFCVIAVNFLFSYPFFKFNFSLDGYPVTFIGM
                     LAITLITSATTTSLKQHQKAAAEREKALAEADKEKLRANLLRAVSHDLRTPLTSIIGS
                     SSSYLENGSDMTEYERTELVSNIKEDSEWLLNMVENLLTITRIDNNSQDKVKKSPEVV
                     EEVVSEAIQRLRKRLSDVRIKVHMPNDFLMIPMDATLIEQVLINLLENASVHSESTEE
                     IDLIITQTKECVSFSVRDYGKGIDPEQLPYIFEGQRSSGKNSDHHKGIGIGLSICKTI
                     IEAHGGKLTAVNHKHGAEFIFTLPKEKEVEANA"
     gene            complement(1781..2965)
                     /locus_tag="ACZPD4_07780"
     CDS             complement(1781..2965)
                     /locus_tag="ACZPD4_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1378988.1"
                     /translation="MLRRTERILENIPVGSLGLISLTGCEELGKKVDDYLVKWRHESG
                     NAFRDDIAFSGYEKDSYLLDARVPRFGSGEAKGVLGESVRGKDLYLMVDVCNYSITYS
                     LAGKTNHMSPDDHFQNLKRIIAAIGGKGRRINVIMPFLYESRQHKRSSRESLDCALAL
                     QELVRMGVENIITFDAHDPRVQNAIPLSGFETVSPTYQFIKGLLRNVKDLQIDADHMM
                     AISPDEGGTNRAVYLANVLGLDMGMFYKRRDYTKIVDGRNPIVAHEFLGSSVEGKDVI
                     IIDDMISSGDSIIDVATELKQRKANRIFAAATFGLFTNGMEKFDKAYEDGIIHGILTT
                     NLIYQTPELLSKPYYINCDMSKYIALIIDTLNHDGSLSSILSPNERIQHVLQKYKNGE
                     PV"
BASE COUNT          831 a          622 c          661 g          919 t
ORIGIN      
        1 gggatcttct tcgatttttc ggcggatatt ggccatgttg acacgaagaa tcttattgtc
       61 attatcagcg taaggtcccc atacatttga aagaatcgat gcatatgtaa tgacttttcc
      121 ggaattttgc gccagatatg ctacaatctt atattcgatc ggagtcagat ggacagcatt
      181 gccgcgaatc gtgagcagcc gtttggaaaa gtcaagtatt aaatccttat gtcggtaagg
      241 acggatatag aaggtgctgt cagtgagcag acgattgctg tgtctgaggg aagcgcggat
      301 ccgtgcaaga agctcggaag ttccgaatgg ttttgtaata taatcatcag cgcccagatc
      361 aagagccgca accttttccc gttcggcagt gcgggcggaa ataacgatga tcggagtact
      421 tgcccatgta cggacgtccc ggataataga acagccgtcc atgtccggaa gtcccagatc
      481 aagaaggata agatccgggc atcgggaacg gatcatttcc agtccttgct taccggtatc
      541 ggcataaaga acctgatagt catgacgttt taaaaccttt cccatgaaag tttgtatatt
      601 tttttcatct tcaatgatta agactgaaaa tttaggcatt tgcttcaacc tccttttctt
      661 ttggaagtgt aaaaataaat tcggcgccgt gtttgtgatt gacagcggtt aatttccctc
      721 catgagcttc tatgatcgtc ttacagattg acagaccgat tccgattcct ttatggtggt
      781 ctgaattttt tccggaactg cgctgacctt caaatatata aggaagctgt tcgggatcga
      841 ttccctttcc gtagtcacgg acagaaaaag aaacacactc ttttgtctgt gtaatgatca
      901 gatcgatctc ttccgtactt tcagaatgaa cggaagcatt ttccagaaga ttaatgagaa
      961 cctgttcgat taatgtggcg tccatgggaa tcataagaaa atcattcggc atatggactt
     1021 tgatacggac gtccgaaagc cgttttctga gccgttgtat agcctctgaa acgacttctt
     1081 cgacgacttc cggagacttc ttcactttgt cttgagagtt gttgtctatg cgcgtgatcg
     1141 taagaagatt ctcgaccata ttcagcagcc attcagaatc ctctttaata tttgatacaa
     1201 gttcggttcg ttcatattcg gtcatatctg atccgttttc caaataagaa gaggaagagc
     1261 cgataatact tgtgagtggt gttcgcagat catgtgatac ggcacgtaaa agatttgcac
     1321 gcagtttttc tttatcagct tctgccagcg ctttttctcg ttccgcagcg gctttttgat
     1381 gttgttttaa agaagttgtt gttgcgcttg tgattagtgt gatcgcgagc atcccgataa
     1441 acgtgacagg atatccgtcc aatgaaaaat taaatttaaa aaatggataa gaaaaaagga
     1501 aattaacggc gatgacgcag aataaagagg cgacaatgcc gtatacatat ccggacgtct
     1561 taagagcggt caaaatgagc gcaagcgtat agatcacagt gatgtttgct gaatttttat
     1621 ttcctaagtg aaagaaacaa aaagaaacag cagtcgcagt gaagagcatg aaagctgtaa
     1681 atagtgtgtc agaaatcgtt ttttttcgtt ctgttttgtt gttttgtctg ttcatagtaa
     1741 tttattcgct taaagtatga aaaaagcatg gagagcggtc ttagaccggc tctccgtttt
     1801 tgtatttctg aagaacatgc tggattcttt cgttcggact taaaatagaa ctaagagatc
     1861 cgtcatgatt caatgtatcg atgataagag caatatattt gctcatatca cagttgatgt
     1921 aatatggttt agacaaaagc tccggtgtct ggtagatcag gtttgtcgta aggataccgt
     1981 gaataatacc gtcttcatat gctttgtcga atttctccat accgtttgta aacagaccga
     2041 atgtagccgc agcgaaaatc cggtttgctt tacgctgttt cagttccgta gcgacatcta
     2101 tgatgctgtc tcctgaagaa atcatatcgt cgatgatgat gacatctttt ccctctacgg
     2161 aagagccaag gaattcatga gcaacgatcg gattacgtcc gtctacaatc tttgtgtaat
     2221 cgcggcgttt atagaacata cccatgtcaa ggccaagtac gttggcaaga tatacggcac
     2281 ggtttgttcc gccctcgtca ggactgatcg ccatcatatg atctgcgtcg atctgtaagt
     2341 ctttgacatt gcgcagaagc ccttttataa actgatatgt cggagaaact gtttcaaatc
     2401 cgctcagcgg gatcgcattt tgtacacgtg ggtcatgagc gtcaaaagta atgatgtttt
     2461 caactcccat gcgtacaagt tcctgcaaag caagagcgca gtcgagtgat tcacgggaac
     2521 ttcttttgtg ctgacggctc tcatataaaa acggcatgat aacattgata cgtcgtcctt
     2581 ttccgccaat ggcagcgata attctcttta agttctggaa atgatcgtca ggagacatgt
     2641 gatttgtctt tcccgcaaga gagtaagtaa tgctgtaatt acatacatct accataagat
     2701 acagatcttt tccgcgaacg gactctccga gaactccttt tgcttctcct gagccaaagc
     2761 gcggaacgcg ggcatcaaga agataagaat ctttttcata tcccgaaaat gcaatatcgt
     2821 cgcgaaaagc atttccgctt tcgtgtctcc attttacaag atagtcatct actttctttc
     2881 ccagttcttc acatcccgtg agggaaataa gaccaagact tcctaccgga atattttcca
     2941 aaattcgttc tgttcggcgt aacattaatg tacctcccta ttttttaatt ttttcttgat
     3001 tcgtatatca tcaccgatta tctttaacaa tgc
//
LOCUS       DBMDXF010000216         3030 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7255_length_3030_cov_1.359850, whole genome shotgun sequence.
ACCESSION   DBMDXF010000216 DBMDXF010000000
VERSION     DBMDXF010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7255_length_3030_cov_1.359850"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            386..1840
                     /gene="guaB"
                     /locus_tag="ACZPD4_07785"
     CDS             386..1840
                     /gene="guaB"
                     /locus_tag="ACZPD4_07785"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1378989.1"
                     /translation="MGKIIGEGITFDDVLLVPAYSEVIPNEVDLSTYLTKKIRLNIPM
                     MSAGMDTVTEHRMAIAMARQGGIGIIHKNMSIEAQAEEVDKVKRSENGVITDPFYLSP
                     ENTLEDANNLMAKFRISGVPITEGRKLVGIITNRDLKFEEDFSKKIKESMTSEGLITA
                     KEGITLEEAKRVLAKARKEKLPIVDDEGNLTGLITIKDIEKQIKYPLSAKDSQGRLLC
                     GAAIGITANCLERVDALVKAKVDVIVMDSAHGHSANVLRTVRMVKEKYPDLQVIAGNV
                     ATGEAAKALIEAGVDAVKVGIGPGSICTTRVVAGIGVPQITAVMDCYNVAKEYGVPII
                     ADGGIKYSGDMTKAIAAGANVCMMGSIFAGCDESPGTFELYQGRKYKVYRGMGSIAAM
                     ENGSKDRYFQENAKKLVPEGVEGRVAYKGTVEDTVFQLMGGLRSGMGYCGTHTIEELK
                     SNGRFIKISAASLKESHPHDIHITKEAPNYSVDE"
     gene            2098..2847
                     /locus_tag="ACZPD4_07790"
     CDS             2098..2847
                     /locus_tag="ACZPD4_07790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR1378990.1"
                     /translation="MSLYKKNRRKVSKNRKNASGHNTGRSSTKKSRSVKSNSLKQKMK
                     KKRIRITAVAGLAVLLILTIILVVRSCIGERNQFVETDASQPDIDVQLLDVNPYSRPG
                     IESEGVKGVVIHYTANPGSTAQDNRDYFNGLQYSGQTEASSNFIVGLDGEIIQCVPTW
                     EIAYASNERNADTVSIEVCHPDETGKFTDATYRSLVQLTAWLCVKFDLKEDQIIRHYD
                     VTGKNCPKYFVENEDAWEMFKDNVWDAVQKK"
BASE COUNT         1028 a          447 c          733 g          822 t
ORIGIN      
        1 ttctttgtat gtagttttga aggtatatgt aaacgagata taaataagaa gagaatattt
       61 tccaaacgga atgagagaag gcagcgtctg aaaaggcgtt gccttttttt gcggcagaga
      121 caagctaaag tcgggcttgt caggaatatt gatttagaaa gaaaaacttc caatgtgtat
      181 aatattaaag tgatatatat tctatatatt atgaaaaatc atggtgaaat tcactatata
      241 agtataactt ataaataaag gttaaaataa atcaatgctg aaaatcgtga gcattctctg
      301 aaagggttga catcagttct gaatttttgt agtatatcaa ataacattta ctagtatgga
      361 ttggtagaaa gagaggtcaa aggacatggg aaagattata ggagaaggta ttacgtttga
      421 tgatgtgctg ttagttcccg catattcaga ggtaattccg aatgaagtag atctgtcaac
      481 atatttgact aaaaagataa gacttaatat tccgatgatg agcgccggaa tggatacggt
      541 tacagaacac cgtatggcga ttgcaatggc tcgtcagggc ggcatcggta ttattcataa
      601 aaatatgtcg attgaagctc aggcggaaga ggtggacaag gtaaaaagat ctgaaaacgg
      661 ggttattaca gatccgtttt atctgtcacc ggagaatact ttggaagacg ctaataattt
      721 aatggcaaaa ttccgtattt caggagttcc gataacagaa gggcgtaaac ttgtcggtat
      781 tattacgaat cgtgatttaa aatttgaaga ggatttttct aaaaaaatta aagaatctat
      841 gacttctgaa ggattgatta cagcaaaaga aggaattact cttgaagaag caaagagagt
      901 tcttgcaaag gcaagaaaag aaaaacttcc gattgtggat gatgagggaa atctgacagg
      961 attgatcaca atcaaagata ttgagaaaca gatcaagtat ccgttgtctg caaaagattc
     1021 gcagggacgc cttctctgtg gagcggcaat cggaattaca gcaaattgtc ttgagcgagt
     1081 agatgctctg gtaaaggcaa aagtagatgt tatcgttatg gattctgcgc atggtcattc
     1141 tgcaaatgtt cttcgtactg ttcgtatggt gaaggaaaaa tatccggatc ttcaggtaat
     1201 tgccggtaat gtggcgacag gagaagctgc gaaagcatta attgaagccg gagttgatgc
     1261 ggtgaaagtc ggtatcggac cgggatctat ctgtacgact cgtgttgttg ccggaatcgg
     1321 tgtgccgcag attactgcgg taatggactg ctataatgtt gcaaaagaat acggtgtgcc
     1381 gattattgca gacggaggca tcaaatattc aggagacatg acgaaagcaa tcgccgcagg
     1441 agcaaacgta tgtatgatgg gaagtatttt tgcgggatgt gatgaaagtc cgggaacatt
     1501 tgaactttat cagggaagaa aatataaagt ataccgtgga atgggttcaa tcgctgctat
     1561 ggaaaacgga agcaaagacc gttatttcca ggaaaatgca aagaagctcg ttccggaagg
     1621 tgtagaaggc cgtgtagctt ataagggaac ggttgaggat actgtattcc agcttatggg
     1681 aggacttcgt tcgggtatgg gatattgcgg aacacatacg atcgaagaac ttaaatcaaa
     1741 cggaagattt attaagattt cagcagcatc tcttaaagaa agtcatccgc atgatattca
     1801 tatcacaaaa gaagcgccaa actatagcgt agacgaataa agaaaatcag catcattaca
     1861 taacagaaaa acggtttgtg tgacgatggg aatcaaagta aatagaaaac gtgatcaaat
     1921 aaatgcctgt tgaatttctg acaggcattt gtttttataa cggcgacgag ccaaagtccg
     1981 ggcttgtcca agactttgtc accgcttata atcccggaat gtgctttttg tcactttcgg
     2041 ggattcatca tatcagcaga ccaaaatgcg aaagatacat agaacagagg caaagaaatg
     2101 agtctttata aaaaaaacag aagaaaagta tcgaaaaaca gaaaaaatgc atcgggacat
     2161 aatactggaa gatcaagtac aaagaaaagc cgttctgtaa aatcgaattc gttaaagcag
     2221 aagatgaaaa agaaaagaat cagaataacg gcagttgcag gactcgcagt gcttcttatt
     2281 ctgacgatta ttttagttgt gcgctcctgt atcggagaga gaaatcagtt tgtagaaaca
     2341 gatgcttcac agccggatat tgatgtacag cttttggatg taaatcccta ttcaaggccc
     2401 ggaatcgaga gcgaaggagt aaaaggcgtt gttattcatt atactgcaaa tccgggaagt
     2461 acggctcagg ataacagaga ttattttaat ggccttcaat attccgggca gacggaagca
     2521 agcagtaatt ttattgtagg attggatgga gagatcatac aatgtgtacc gacatgggag
     2581 atcgcttatg catcgaatga gcgaaatgca gatacagtat cgattgaagt ctgtcaccct
     2641 gatgaaacag gaaagtttac tgatgcgaca taccgttctc ttgtacagct tacagcatgg
     2701 ctttgtgtca aatttgattt gaaagaagat cagattattc gccattacga tgttacagga
     2761 aaaaattgtc cgaaatattt tgtagaaaac gaagatgcat gggaaatgtt taaagataat
     2821 gtgtgggacg cggttcaaaa gaaatagatg atccgcttgt ttggacaatt atattgagga
     2881 cagtttcttt gattttcgga aaatttgcgc agactgtatt tgtaaaatcc cttgtcatgt
     2941 ttctatggaa ttgatataat gtgttagatt tgttagtttg agtgaaatgt ggagggtaaa
     3001 gatgaacgat ctggaaatgt atcgtgaaca
//
LOCUS       DBMDXF010000217         3025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7267_length_3025_cov_1.800274, whole genome shotgun sequence.
ACCESSION   DBMDXF010000217 DBMDXF010000000
VERSION     DBMDXF010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7267_length_3025_cov_1.800274"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2943)
                     /locus_tag="ACZPD4_07795"
     CDS             complement(<1..2943)
                     /locus_tag="ACZPD4_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1378991.1"
                     /translation="MRDWKRWTAVLLAGSLAFTAADDMKLIVQAYEMQNEQGQESYQA
                     ESKSKLDDLKEKAVLFQEFQGEEQRFDGTRAVDVSDHAEQIHKIETGSVVFRFKASKK
                     ADGVLLGAKDKTIDLPTDLNRGSDCTSFFIRANEKFRMVYKHTAAEHVGPASFSDGNW
                     HTVVVSSQNEKSMRLTIDGQEMWSNTDAGNRGLFSKQSVLDQVTIGAQKTKDGQVYKG
                     FQGEISHVIITSETLTDADAIAISKPETSGEIASGSAVGEMFQIQYGDNSWVFTGGEA
                     VQGGFAQTRGVRNYVGQFEEYVRWTKAGNENGRQRYTINTGKAGQTLKDVVDNYQTLV
                     ADYSPKAAAYLVGKEDYQAGEAGIASFQDSLRQFINLSLGLKENGKGFAVIQKPFAVK
                     DDAVNATIMLYCKAVDEVVKEYEDESEKLDRIVVVDHFAQTNQDDFKNNKLKDGQTLN
                     AAGHFEIGKQFSAATIKTTDSYPGNGVTLNLKEEEQPDVYLNVLPVVTAENAGLHVQI
                     PETNETSWRYELSIGDKKITGSADGNTFTITGAESGKEYLFKCISSDGTTQLQTVTGK
                     TEAGNVGIAYGQTLDEKQKALSEKLKEKDKMTWLFMGDSITHAALWTKGYDGIAQTFE
                     KYLKDEMGRASDTVINTAVSGATTTSTLNNIEQRLEKYTPDVVSIMLGTNDAATGGLT
                     ADIYKKNLETIIEKIRNKNKDAVIILRTPTPMWNTGSREANIPQYIAKMKQVADEQNL
                     IYIDQYTELQKAFNDYGWLKKDTVLFGNNLHPGANGHLLMTRHFLKGCGLWKEDSAIA
                     NLFYEMPINEKTSEITPEVIKTPNRIGVSLEKLKEDSKSQIGAVHLKAVSKASGQTYE
                     TDAEAGEKLIVLKNLPENQKYEVEVSAWLKDRAEKTVFQKQEIELNNTLEEAFDICLS
                     DEKVENLNEGTTVGTFTVNEMPPEGNYVFSLCTGEGDTHNPYFAIENGVLKTAKKLEE
                     GKTYTIRLK"
BASE COUNT          667 a          803 c          507 g         1048 t
ORIGIN      
        1 tttcagccgg atcgtatatg tctttccttc ctccagtttc tttgctgttt tcaacactcc
       61 gttttctatc gcaaaatatg gattatgagt gtcgccttct cctgtgcaaa gagagaaaac
      121 atagtttcct tcaggaggca tctcattaac tgtgaatgtt cccaccgttg ttccttcatt
      181 caagttttct actttttcat cactcaaaca aatatcaaat gcttcctcaa gcgtattatt
      241 taattcaatc tcctgttttt gaaatactgt tttttccgcc cggtctttca gccatgcaga
      301 cacttctacc tcgtatttct gattctcggg aaggtttttt aaaacaatca acttctctcc
      361 tgcttctgca tctgtctcat atgtctgccc tgatgctttg gaaaccgctt tcagatgcac
      421 cgcccctatc tgagacttgc tgtcttcttt cagcttttcc aaagacacgc cgatgcggtt
      481 cggcgttttt ataacttccg gagtgatttc agatgttttc tcattgatcg gcatctcata
      541 aaacaaattg gcaatcgcgc tgtcctcttt ccacaatccg catcctttta aaaagtgtct
      601 ggtcataaga aggtgtccgt ttgcgcccgg atgcagatta tttccgaata aaacagtatc
      661 ttttttcagc cagccgtaat cgttaaacgc tttttgaagt tccgtatact gatcgatata
      721 gatcaggttc tgctcgtctg cgacctgctt catttttgca atatactgcg gtatattcgc
      781 ttctcttgaa ccggtattcc acattggcgt tggcgttctt aaaatgatca ccgcgtcttt
      841 atttttattt ctgatcttct ctatgatcgt ctcaagattc tttttgtata tatccgccgt
      901 aagtcctcct gttgccgcat cattcgttcc aagcatgatc gagactacgt caggagtata
      961 tttttccaat ctctgttcaa tattattcag cgtacttgtt gtcgtcgctc ctgaaacagc
     1021 cgtattgatc acagtatctg aagctcttcc catctcatct ttcaaatatt tttcaaaagt
     1081 ctgagcgatc ccgtcataac cttttgtcca caaagccgca tgtgtaatag aatctcccat
     1141 gaacagccat gtcatcttat ctttttcttt cagcttttcc gaaagcgctt tttgcttttc
     1201 atccagcgtc tgcccatacg cgattccgac attgcctgct tccgtttttc ctgtcacagt
     1261 ttgaagctgt gtcgttccgt ctgatgaaat acatttgaaa aggtactctt ttcccgattc
     1321 tgctcctgta atcgtaaatg tattcccatc cgcacttccc gttatttttt tatctccaat
     1381 actcaactcg tatctccacg acgtttcatt tgtttccggg atctgtacat gcagaccggc
     1441 attctccgcc gtcacaaccg gaagaacgtt caaatatacg tccggctgct cttcttcttt
     1501 cagattcaga gtcactccgt ttcccggata actgtccgtc gtcttgatcg tagctgcgga
     1561 aaactgtttt ccgatctcaa aatgtcccgc tgcgttcagc gtttgaccgt cttttaattt
     1621 attattttta aaatcatcct gatttgtctg tgcaaaatga tcgactacaa cgatccgatc
     1681 gagcttctcc gactcgtctt cgtattcttt tacaacttcg tctaccgctt tacagtacag
     1741 catgattgtt gcatttaccg catcatcttt aactgcaaat ggcttttgga tcacagcaaa
     1801 tccttttccg ttttctttta atcctaatga caggttgata aattgacgca atgaatcttg
     1861 aaacgaagca attcctgctt ctcctgcctg ataatcctct ttcccgacga gataagccgc
     1921 cgctttcggg ctgtaatccg ccactaaagt ctgataattg tcaacgacgt cttttaaagt
     1981 ctgaccggcc tttcctgtgt tgatcgtata acgctgccgc ccgttttcat ttcctgcttt
     2041 cgtccaccgc acatactctt caaactgtcc tacataattt cttacccctc ttgtctgggc
     2101 aaatcctccc tgtacggctt ctcctcctgt aaatacccat gagttatcgc cgtactgaat
     2161 ttgaaacatc tcgcccactg ccgaaccgct tgcgatctca ccggaagttt ccggtttcga
     2221 tatcgcgatc gcgtccgcgt ccgtcaacgt ttcacttgta atgataacgt gagaaatctc
     2281 cccctgaaat cctttgtaaa cctgtccgtc ttttgttttc tgcgctccga tcgtcacctg
     2341 atccagaacc gactgctttg aaaaaagacc tctgttccct gcgtctgtgt tactccacat
     2401 ttcctgtccg tcgatcgtca gacgcatcga cttctcattc tgactgctga caacgacagt
     2461 gtgccaattc ccatctgaaa atgaagccgg tcctacatgt tccgccgcag tatgtttata
     2521 taccatacga aacttttcat tcgcccttat aaagaaagaa gtacagtcgc ttcccctgtt
     2581 aagatctgtc ggaagatcga tcgttttatc tttcgctccc agtaaaacgc cgtccgcttt
     2641 tttggaagct ttaaatctga acactacgct tcccgtctct attttatgta tttgttccgc
     2701 atgatcgctg acatcgacgg cgcgcgttcc gtcaaatctc tgttcttcgc cctgaaattc
     2761 ctgaaagaga actgcttttt cttttaaatc gtccaattta cttttgcttt ccgcctgata
     2821 cgattcctgt ccttgttcat tctgcatttc atatgcctga acaatcaatt tcatatcatc
     2881 tgcagctgta aacgctaaag agcctgccaa aagtacagca gtccaccgct tccaatccct
     2941 catttccttt cctccattct ttttgtgaat tgtagatatt ttttccggta tttcagaatt
     3001 attttggtat attatgcacc catta
//
LOCUS       DBMDXF010000218         3003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7329_length_3003_cov_1.610613, whole genome shotgun sequence.
ACCESSION   DBMDXF010000218 DBMDXF010000000
VERSION     DBMDXF010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_3003_cov_1.610613"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            283..570
                     /locus_tag="ACZPD4_07800"
     CDS             283..570
                     /locus_tag="ACZPD4_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1378992.1"
                     /translation="MKLVPLGDKIVLKQLEAEETTKSGIVLPGQAKEKPQEAEVIAVG
                     PGGNIDGKEVVMQVNVGDKVIYSKYAGTDVELDKEEYIIVKQSDILAIVTE"
     gene            622..2247
                     /gene="groL"
                     /locus_tag="ACZPD4_07805"
     CDS             622..2247
                     /gene="groL"
                     /locus_tag="ACZPD4_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738769.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1378993.1"
                     /translation="MAKEIKYGSEARTALEKGVNQLADTVKVTLGPKGRNVVLDKSFG
                     APLITNDGVTIAKEIELEDGFENMGAQLIREVASKTNDVAGDGTTTATVLAQAMVHEG
                     MKNLEAGANPIVLRKGMKKATDNAVEAIRAMSSKVNGKEQIARVAAVSSGDEEVGQMV
                     ADAMEKVSNDGVITIEESKTMQTELDLVEGMQFDRGYISAYMATDMEKMEAILDDPYI
                     LITDKKISNIQDILPVLEQIVQSGARLLIIAEDIEGEALTTLIVNKLRGTFNVVAVKA
                     PGYGDRRKEMLQDIAILTGGQVISEELGFDLKETTMDQLGRAKSVKVQKENTVIVDGC
                     GDKKAIEDRIAQIKKAIEETTSEFDKEKLQERLAKLAGGVAVIRVGAATETEMKEAKL
                     RMEDALNATRAAVEEGIIAGGGSAYIHASKEVAKLTEELEGDEKTGAKIILKALEAPL
                     HQIAANAGLEGAVIVNKVRESDPGVGFDAYAEEYVDMVSKGILDPAKVTRSALQNATS
                     VASTLLTTESVVATIKEDVPAMPAGGAGGMGMM"
     gene            2369..2911
                     /locus_tag="ACZPD4_07810"
     CDS             2369..2911
                     /locus_tag="ACZPD4_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1378994.1"
                     /translation="MSKKVLVISTSLRKNSNSEKLAEAFASGAEDAGNVVEFLSLKDK
                     TIAFCEGCMACHKLGRCVIDDDANMIARKMYEAEVIAFATPIYYYEMSGQMKTMIDRA
                     NSLYDSDYKFTDIYMLTTAAEDEPEVPNRAIGGLTGWIDCFDRAHLSGSVFAGGVNES
                     GTIKGHSALKKAYEMGNCIK"
BASE COUNT         1020 a          441 c          804 g          738 t
ORIGIN      
        1 tttttacaat gtatatgaag gcagaaaaat gacgaaacgt gcgtagatgc gtttcgtctt
       61 ttttatgggc gatgagccaa agatcgggct tactgaaata ttgtcaactg ctgattgatc
      121 tataatgtgc gttttggaac ttccggtgat tgttgttatt ttttctataa aaacctcata
      181 aactgtgttg acaaattgaa aacagagtgc tattttatat tcatgaagtt tagcactcgt
      241 aataaatgag tgctaacagg caaatttaaa ggaggaatta caatgaaatt agtaccgttg
      301 ggtgacaaaa ttgttttgaa acagttagaa gcagaagaga cgacaaaatc cggtattgtt
      361 ttgccgggac aggcaaaaga aaaaccgcag gaagcagaag ttatcgctgt aggaccgggc
      421 ggaaatatag atggaaaaga agtagtaatg caggtaaacg tgggagataa agtgatttac
      481 tctaaatacg caggaacaga cgtggaactg gacaaagaag agtacattat cgtaaaacag
      541 agcgatattc tcgcaatcgt aacagagtaa aaatagtcag aagacagatg atcacaggta
      601 agaataagga gatgtttaac gatggcaaag gaaatcaaat acggatctga agcaagaaca
      661 gcccttgaaa aaggtgtaaa tcagttagcg gatacagtaa aagtaacact cggaccaaaa
      721 ggaagaaatg tcgttcttga caaatctttc ggagcgccgc ttattacaaa cgatggtgtg
      781 acgattgcaa aagagatcga attggaagac ggatttgaaa atatgggagc gcagcttatc
      841 agagaagttg cttctaagac gaacgatgtg gcaggagatg gaacaacaac ggcaacagtg
      901 cttgcgcagg caatggtaca tgagggaatg aaaaaccttg aagcaggagc gaatccgatc
      961 gttcttagaa aaggtatgaa aaaggcaaca gataacgcgg ttgaagcgat ccgtgctatg
     1021 agcagcaagg tgaatggaaa agaacagatc gcgagagttg cggcagtttc ttccggcgat
     1081 gaggaagtcg gacagatggt tgcggatgcg atggaaaaag tatcgaatga cggagtcatc
     1141 acgattgaag aatccaaaac tatgcagaca gaattagacc ttgtagaagg aatgcagttt
     1201 gatcgaggat atatttctgc atatatggca acagatatgg aaaagatgga agcgattctt
     1261 gatgatccgt atattttgat tacagataag aagatttcta atattcagga tattcttccg
     1321 gtgcttgagc agattgtcca gtccggcgca agacttctta ttatcgcaga ggatattgaa
     1381 ggagaggctc tcacaacatt aatcgtaaat aaacttcgcg gaacatttaa tgttgttgca
     1441 gtaaaagctc cgggttatgg agacagaaga aaagaaatgc ttcaggatat cgcaattttg
     1501 acaggcggac aggtgatctc agaagaactt ggatttgatc tgaaggaaac aactatggat
     1561 cagcttggcc gtgcaaaatc tgtaaaagtt cagaaagaaa atactgttat tgtagatgga
     1621 tgcggagata aaaaagcgat tgaagacaga attgcacaga tcaagaaagc gatcgaggag
     1681 acgacttctg agtttgataa agaaaaattg caggaaagac ttgcaaaact tgcaggcggt
     1741 gtagctgtta tccgtgtcgg cgctgcaaca gaaacggaga tgaaagaagc taagcttcgt
     1801 atggaagatg cactgaatgc gacaagagcg gccgtagaag aaggtattat tgcaggcggt
     1861 ggttctgcgt atatccatgc ttcaaaagaa gtggctaaat tgacagagga attggaaggt
     1921 gatgagaaga caggagccaa gatcattctg aaagcattgg aagctccgct tcatcagatt
     1981 gctgctaatg caggacttga gggcgctgtg attgtaaata aagtaagaga atccgatccg
     2041 ggagtaggat ttgacgctta tgcggaagaa tatgttgata tggtaagcaa aggaattctg
     2101 gatccggcta aagtgacaag aagtgctttg cagaatgcaa cgagcgttgc atccacgctt
     2161 ttgacaacag aatctgttgt ggcaacgatt aaagaagatg taccggctat gccggcaggc
     2221 ggtgcaggcg gaatgggtat gatgtaaaaa tagtatctaa ccatattata gtgtatagaa
     2281 ggtctgtggg aatttcctac agacctttaa aaaatgaaaa ttgaaatacc ggtaccgata
     2341 gtgtattagg tgtttgggag ggcggattat gagtaagaaa gttcttgtta tcagtacgag
     2401 tctgagaaag aatagtaatt cggaaaaact tgcagaggca tttgcatcag gggctgagga
     2461 cgccggtaat gtagtggaat ttctttcctt aaaagataaa acaatcgcgt tttgtgaagg
     2521 gtgtatggcg tgccataaac tgggacgttg tgtgatcgat gatgatgcga atatgattgc
     2581 ccgaaaaatg tatgaggcag aggttatcgc atttgcaaca ccgatctatt attatgaaat
     2641 gagcggacag atgaagacaa tgatcgatag agcaaatagc ttgtatgatt cggattataa
     2701 atttacggat atttatatgt tgacaactgc tgcggaagat gaaccggaag ttccgaatcg
     2761 ggcgatcggt ggtttgacag gatggataga ttgttttgat cgagcgcatc tttcaggaag
     2821 cgtatttgcc ggaggagtaa acgaatcagg aacgatcaaa gggcatagtg cactgaaaaa
     2881 ggcgtatgag atgggaaatt gtattaaata gcaaatgaca aatctgcaag gggtaaggtc
     2941 tcggtgagcg gattttgaat gagtattttg aaagcaatat ttatgactga aaagaagcgg
     3001 taa
//
LOCUS       DBMDXF010000219         3000 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7341_length_3000_cov_1.628148, whole genome shotgun sequence.
ACCESSION   DBMDXF010000219 DBMDXF010000000
VERSION     DBMDXF010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7341_length_3000_cov_1.628148"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="ACZPD4_07815"
     CDS             complement(<1..733)
                     /locus_tag="ACZPD4_07815"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378995.1"
                     /translation="MFACRNLNLSYKGNKILKDIHFHIPEGTVFCITGESGSGKSSLL
                     KVINGIIPEITEAEISGEIKLNDMRLNEMDIAQRSRYISTVYQNPKTQFYCVESTDEL
                     AFPLENRNLPKDEMVKIIKKYTELLDTEKLLNRNIFSLSGGEKQLMAITSVMTMDNQV
                     YLFDEPSASLDHHSIGLLKKCIAELKRKGKIVIIAEHRLYYLKEIMDYLAVLKEGKLE
                     KTDDVNIIPCSLSTASGYNDYCPIPL"
     gene            complement(720..1436)
                     /locus_tag="ACZPD4_07820"
     CDS             complement(720..1436)
                     /locus_tag="ACZPD4_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIR1378996.1"
                     /translation="MHSEKFKLDFRTKMLMSLCLSYTLILGNIQTKFSIVAAAYSLLP
                     YVFLLLNREKKAAVKGILFIMAAYIIQKEWVFSAGGFLGSLFLFITMMFLRLLPGFVM
                     ARFALASSTMSHMTSALKKMHFPVVLIIPITVMARFFHTAREDYNQVKDAMYLHGLTN
                     RRLFFHPVKLFEYRMVPLFMVLTKTADDVAVSAMTRGLKIHGKRTYIGESRFCMRDYI
                     CFLLMLVLIGFYIGGKYVCV"
     gene            complement(1438..2046)
                     /locus_tag="ACZPD4_07825"
     CDS             complement(1438..2046)
                     /locus_tag="ACZPD4_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HIR1378997.1"
                     /translation="MDKKEKTGVLNVRDLVNVGLFSVLIFIATFLSGMIGFIPVLMPV
                     VPFVYGIVSGPVYMLYFTKIKKAGMLFIQTIVVTLAFVATGHGPWVLLTAVIGGLLGE
                     VVLRNGRYKSVKHARLAFSVQSIYGLGNWLPIYFARDAYIRQMLDMGYGEEYTQKMMS
                     VLPNWTLPLIVILGMLGAYIGCTIGIRMLKKHFVKAGMAEEA"
     gene            complement(2120..>3000)
                     /locus_tag="ACZPD4_07830"
     CDS             complement(2120..>3000)
                     /locus_tag="ACZPD4_07830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1378998.1"
                     /translation="ASIYLPIEGIIAFMGMIDMLDSVVGRIKEIKTMPIQEGESKFHP
                     KNYDIEFKDVSFGYEDYSVVRNVSFTAKQGEVTALIGASGSGKTTLTKLAARFWDINK
                     GKILLGGEDISKVDPEALLKNFSIVFQEVVLFNTSIKENIRIGKKGATDAEIFEAAKI
                     ARCDEFIEKMPNGIETVIGENGERLSGGERQRISIARAILKDAPIILMDEATASLDVE
                     NESLIQEALSELIREKTVIVIAHRMRTIRSADKIVLLNNGEIEAIGNDKEMYERSAAY
                     RKMLSDSKIEQTVTVN"
BASE COUNT          860 a          726 c          458 g          956 t
ORIGIN      
        1 ccaatggaat cggacaatag tcattgtatc cactggcggt agaaagtgaa caaggaataa
       61 tgttaacatc atccgttttt tccagttttc cttcttttaa aacagccaga taatccatga
      121 tctctttcag ataatacagt ctgtgttcgg caattatcac gattttccct tttcgtttca
      181 gctcggcgat gcattttttc aaaagaccga tcgaatgatg atccaaagaa gccgacggct
      241 catcgaaaag atatacttga ttgtccatcg tcatcacact tgtaattgcc ataagctgct
      301 tttctccgcc tgacagtgaa aaaatatttc tgtttaaaag cttttccgta tcgagcagct
      361 ccgtatattt ttttattatc ttaaccatct catccttcgg cagatttctg ttttccaaag
      421 gaaacgccag ttcatccgta gactcaacgc aataaaactg tgttttcgga ttttgatata
      481 cggtcgaaat ataccggctt ctctgtgcaa tatccatctc atttaaccgc atatcgttta
      541 attttatttc tcctgaaatt tccgcctcgg taatctcagg gattatccca ttgattactt
      601 ttaataaact gcttttgccg gatccggatt ctcccgttat acaaaaaacc gttccttcag
      661 gaatatgaaa atgtatatct tttagtattt tgtttccttt atatgacaga tttaaatttc
      721 tacacgcaaa catatttccc tcctatataa aatcctatca acacgagcat aagcaaaaaa
      781 caaatgtagt ctctcataca aaacctgctt tcgccgatat acgttctttt cccatggatt
      841 ttcagtcctc gcgtcatcgc cgatacggca acatcatctg ctgtttttgt aagcaccata
      901 aataatggaa ccattctgta ttcaaaaagc tttacaggat gaaaaaagag gcgtctgttt
      961 gtaagaccat gtaaatacat tgcgtctttt acctgattat aatcttctct cgccgtatga
     1021 aaaaatcttg ccataaccgt aatcgggatg atcaacacaa ccggaaaatg cattttttta
     1081 agcgctgacg tcatatggct cattgtagat gaagcaagag caaatctcgc catcacaaat
     1141 cccggaagaa gccgcaaaaa catcatcgta ataaaaagaa ataaagatcc caaaaaacca
     1201 ccggctgaaa acacccattc tttttgaatg atgtaagcag ccataataaa caggattcct
     1261 tttaccgccg ctttcttttc cctgttcaag agtaaaaata catatggcag cagcgaataa
     1321 gctgccgcca caatcgaaaa tttcgtttgt atatttccca ggatcagagt atacgaaaga
     1381 cacaatgaca tcaacatttt cgtccgaaaa tccagtttaa atttttcact gtgcacgcta
     1441 tgcctcctct gccatgcctg cttttacaaa atgtttcttc aacattctta tgcctatcgt
     1501 gcagcctatg tacgcgccga gcattcctaa aatcacaatc agcggaagcg tccagttcgg
     1561 caaaacactc atcatttttt gtgtatattc ttccccgtac cccatatcaa gcatctgccg
     1621 gatataggca tctctcgcaa aataaatcgg aagccagttt ccaagtccat atatggactg
     1681 tacgctgaaa gcaagtcttg catgctttac acttttatac cgtccattcc ttaggacaac
     1741 ttctcccagt aaaccgccga taactgccgt aagcagtacc cacggtccat gtccggttgc
     1801 cacaaatgca agtgttacta cgatcgtctg aatgaaaagc atccctgctt ttttaatctt
     1861 cgtaaaataa agcatgtata ccggccctga cacaatccca taaacgaacg gaacgaccgg
     1921 cattaagacc ggaataaaac cgatcatccc agaaagaaac gtagcaataa aaatcaacac
     1981 ggaaaataat ccgacattta ccaagtctct cacatttagt acgcctgttt tttctttttt
     2041 atccattatc tttttctccc aactatacga atcatgttaa gcattgcagt atcaaataaa
     2101 tccacttcgg ataatagctt taattaactg taactgtttg ttctatttta gaatcggata
     2161 gcatttttct atatgcggcc gaccgttcat acatttcttt atcatttcct atcgcctcga
     2221 tttctccgtt gttcagaaga acgattttat cagcgcttcg gatcgttctc attctgtgag
     2281 ctatcacaat cactgttttt tctctgatca gctccgacaa cgcctcctga ataagacttt
     2341 cattttcaac atctaaagat gcggttgctt catccatcaa tatgatcgga gcatctttca
     2401 gtatggctcg tgcaatggaa attctttgcc tttcccctcc tgaaagccgt tctccgtttt
     2461 cacctattac agtctctatt ccatttggca ttttttcgat aaattcatcg catcttgcta
     2521 ttttagccgc ctcaaaaatt tcagcatctg tcgcaccttt tttcccgatc cttatattct
     2581 ctttgatact tgtgttaaat aacactacct cctgaaaaac aatagaaaag ttcttcagca
     2641 gcgcttcagg atctacttta cttatgtcct ctcctcccag caagatcttt cctttattaa
     2701 tatcccaaaa tcttgcggca agctttgtaa gagtagtctt tccacttccg gatgccccga
     2761 tgagcgccgt tacctctcct tgttttgccg taaaacttac attcctgaca accgaataat
     2821 cctcatatcc aaatgaaacg tctttaaatt ctatatcata atttttcgga tgaaactttg
     2881 attccccctc ttggatcggc atcgttttta tttcttttat tctgcctaca acactgtcaa
     2941 gcatatcgat catccccata aaggctatga tcccttctat cggaagatag atacttgcag
//
LOCUS       DBMDXF010000220         2981 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7381_length_2981_cov_2.057986, whole genome shotgun sequence.
ACCESSION   DBMDXF010000220 DBMDXF010000000
VERSION     DBMDXF010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7381_length_2981_cov_2.057986"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="ACZPD4_07835"
     CDS             <1..648
                     /locus_tag="ACZPD4_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1378999.1"
                     /translation="HRVEAAFINIELNGMDGLQLGEKLRELNPKIVLVFFIEHTEYIL
                     EALRIKADGYMIRPYTMEDLTWTMGNAKLLSRRQKKPAYIRTFGRFDLFINGELVIFS
                     NKKAKELLALCVDNKGGVVTMEEAVDKLWENRLYDEKVKNLYRRAVMDLNKIFSTYGI
                     KDVFVKKRAACWINYANVDCDYYMWIEENILSGTGKSVRSGIDTRGGVYDGVFMG"
     gene            1044..>2543
                     /locus_tag="ACZPD4_07840"
     CDS             1044..>2543
                     /locus_tag="ACZPD4_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1379000.1"
                     /translation="MRKKKNSKLRSALAIFLAGAMVLGMIPGGMGQVYASDSTPTPAV
                     AFDETSVSDPSTVDTWNQVVKDSTENIGRIWTDKTVLTEDIQLEGGSRPSVKKGDSDF
                     LVGLSALSSTSNTVKTVSRPLDIVLVVDTSGSMENSPHMGTRPESERYRYEEIYAGNL
                     SETEDQEYYTKDGGKITSGGQKILFWWEFTHWELNGQTVEPKISAEDSNPNHIQFYER
                     RDLGTNITKLQALQNAVNNFVEQTAKMNDSIDDIKLQHRVSLVKFASDESDNIGNDFI
                     NNNYNRSQIVTELKSYTTKNISDLTSTVNSLIAAGATRADFGLNQAQRAFQLGGTREG
                     AQKVVVFFTDGQPTSNSDWSNSVAAAAITNAKELKDANALIYSIGVFRDANPNDTNTS
                     AGNFNGYMHAVSSNYPDATATSSTRPNRYSCTLGKRTDNSDYYKAATDADELNNIFNE
                     ISSDLETGFGFPTQTEGYDPGKVGYITFTDQLGEYMQVDEFKSLVFADQI"
     assembly_gap    2544..2553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2554..>2981
                     /locus_tag="ACZPD4_07845"
     CDS             <2554..>2981
                     /locus_tag="ACZPD4_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379001.1"
                     /translation="FDPVGEPVTENGVTTYKYEGSATGDTELYPNGNVKDIIVQVKKG
                     ADLKTGDTVTVQIPAGLIPLRHFTLDTDSDGNKTMDIQEAYPLRIFYGVSIKPEVRER
                     IADGLNPNDADDEALQQYLNAHNENGVPYFYSNYYNGQYI"
BASE COUNT         1016 a          457 c          713 g          785 t
ORIGIN      
        1 catagagtgg aagcggcttt tataaatatc gaattaaatg gtatggacgg acttcagctc
       61 ggagaaaagt tacgggaatt aaatcctaaa atagttcttg tattttttat agaacataca
      121 gaatatatat tggaggcact tcggataaaa gcggatggtt atatgatcag gccgtatact
      181 atggaagatt taacatggac tatgggaaat gcaaaacttc tcagtcggcg acagaagaag
      241 ccggcatata ttaggacatt tggccgattc gacttattta ttaatggaga acttgtgata
      301 ttttccaata aaaaggcaaa agaactgctt gcgttatgtg tggacaataa aggcggagtt
      361 gtgacaatgg aagaagccgt tgacaaactc tgggagaatc gtttgtacga tgaaaaggta
      421 aaaaatctgt atcgcagggc ggttatggat ttgaataaga tattcagtac atatgggatt
      481 aaagatgtat ttgttaagaa acgggctgcc tgttggatta attatgccaa tgtggactgt
      541 gattattata tgtggataga agaaaatata ttatccggta cagggaaaag tgttagaagc
      601 ggcattgata cccggggggg ggtatatgac ggagtattca tgggctgaag aaacaaatgc
      661 ccgaatcaac aggtataaat tcagggaaga taactaaaaa cagagaaaaa gtaaaaccga
      721 tttattgtag aaattattaa aatttcgaca atagatcggt ttttcacata tagtggatta
      781 ttaatgcgga aaatgtgaaa aagtaaaaat ataatagctt gaaaaaggag aaaatggcaa
      841 gttattcaca ctttgttaac gtatgatttt atatgctgtt gaaaagatgg agctattttg
      901 cattatttag aaaaaatatg taaggggagt gatgaattta gtgaaatgat gtcaaatact
      961 ccattgattt tatatagatt gatgcagggt taataggaat ataaataatt ctgtaaataa
     1021 gcaagaaagg aaagatagtg actatgagga aaaagaaaaa tagtaaactg cgttcagcgc
     1081 tggcgatttt tcttgccggg gctatggtgc tcggaatgat acccggtggt atgggacaag
     1141 tctatgcctc agattcgact ccgactccgg cggtagcgtt tgatgaaacc tctgtgtcag
     1201 atccgtctac tgtggatact tggaatcagg ttgtaaaaga ttcgacagaa aacatcggac
     1261 gtatctggac agataaaaca gtactcacag aggatattca gcttgagggc ggaagcagac
     1321 caagtgtcaa aaaaggcgat tctgatttct tagttggatt atcggcattg tcatctacat
     1381 cgaatacagt aaagacggtt agcagaccgt tggatatcgt actggtagta gacacgtcag
     1441 gttctatgga aaattcaccg catatgggaa ctaggcctga gagtgagcga tatcgatatg
     1501 aagagatata tgcaggaaat cttagtgaaa cagaagatca agaatattac acaaaggatg
     1561 gaggcaaaat tacttctgga ggtcaaaaaa ttctgttttg gtgggaattt acgcactggg
     1621 aattaaatgg acaaacggtc gagccgaaga tatccgcaga agattccaat ccaaatcaca
     1681 tccaatttta cgagagaaga gatttgggaa caaatattac gaagctgcaa gctctgcaaa
     1741 atgcagtaaa taactttgtg gaacaaacag ctaaaatgaa tgattccatt gatgatataa
     1801 aattacagca tcgagtttcc cttgtaaaat ttgcaagtga tgaaagtgac aacattggaa
     1861 atgattttat aaataacaat tataatcgtt cacaaattgt aacggaatta aagtcttaca
     1921 caacgaaaaa tatttcggac ttgacctcaa cagtgaatag tctgattgca gcaggagcaa
     1981 ctcgcgcaga ttttggattg aatcaggcgc agagagcatt tcagcttgga ggaaccagag
     2041 aaggggcgca gaaagttgtc gttttcttca cagatggtca gccgacgtcg aatagtgact
     2101 ggagtaacag tgttgcagct gccgccatta cgaacgcaaa agagttgaag gatgcgaatg
     2161 ctttgatcta cagtatcggc gtgttccggg atgcgaatcc gaacgataca aatacaagcg
     2221 ctggtaactt taacggatat atgcatgcgg tttctagcaa ctatccggat gcgactgcta
     2281 caagcagtac acgacctaat cgctattctt gtacattagg aaaacgcaca gataattctg
     2341 attattataa agcggcaacg gatgcagatg aattgaataa tatatttaat gagatttcat
     2401 cggatttgga aacaggattt ggttttccga cacagacaga agggtatgat ccgggtaagg
     2461 taggatatat tacatttacc gatcagctcg gcgaatatat gcaggtggat gaatttaaga
     2521 gtttggtctt tgcagatcag atannnnnnn nnntattcga tccggtaggt gaaccggtta
     2581 ctgaaaacgg tgttactaca tataaatatg aaggaagtgc aacaggagat acagaacttt
     2641 atccgaacgg taatgtaaaa gatattatcg ttcaggtgaa gaaaggggca gacctgaaaa
     2701 ccggtgatac agttacagta cagattccgg caggtttgat cccgcttcgt cactttacgt
     2761 tagatacaga cagtgacgga aataaaacga tggatataca ggaggcatat ccgctccgta
     2821 tcttctatgg tgtcagcatc aagccggagg tccgggaaag aattgccgac ggactaaacc
     2881 cgaatgatgc agatgacgag gcgcttcagc aatatctgaa cgctcataat gagaatggag
     2941 ttccgtattt ctattcaaac tattataacg gacaatacat a
//
LOCUS       DBMDXF010000221         2973 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7395_length_2973_cov_2.252089, whole genome shotgun sequence.
ACCESSION   DBMDXF010000221 DBMDXF010000000
VERSION     DBMDXF010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7395_length_2973_cov_2.252089"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="trkA"
                     /locus_tag="ACZPD4_07850"
     CDS             complement(<1..1155)
                     /gene="trkA"
                     /locus_tag="ACZPD4_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR1379002.1"
                     /translation="MKIVIIGDGKVGYKLARQLSADDYDIVMIDSNERKLRFAIDRLD
                     IFCVHGNGVDADVQKAADVPHADLVIACTSADECNMLSCLIARKLGARHTIARVRNPI
                     YFKQIGLLKEELHLSMAVNPELIVANDISRLLLFPVASKIETFVKGRVELIEFTVSED
                     NRLVGMSLAAIYKKFQIKILVCAVEHEGKVFIPDGEYVLQAGDKLHITGAHEAMEAFF
                     KMHGKRQLQTKIKKVLICGGGRVAYYLAARLCKIGMHVKIIEKDENRCEELCDCLPKV
                     TVIHGDATDHDLLSEEGVENVDAFVALTGIDEENIILSLYAKSQGAGKIIAKVNEDRR
                     ARMVADFGIDSIVSAKTATADAILSYVRARKNSQKSANVETMYQLVDEKVE"
     gene            complement(1419..>2973)
                     /locus_tag="ACZPD4_07855"
     CDS             complement(1419..>2973)
                     /locus_tag="ACZPD4_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1379003.1"
                     /translation="YINVIKNAGRRYTEIGQREECEIPDYCKPNLEILRDSLMEAVAE
                     TSEEFMERYFNGEEFSVEEIRSAMRTEVMDGDIVPVAMGSNVQAQGVANLLSDIVRFF
                     PSPDKRKCAGINRRTNEIFEGNYEFSKAKTAYVFKTMVDPFIGKYSFVKVCSGVLKGD
                     DILYNADADAEEKPGKIYTMCGNKPAEVSELFAGDIGAIAKLGSTRTGDTLSTKANPV
                     SYAKTDYSVPYTYMKYTVKTKGDEDKVSQALAKMTAEDVTLKSVNDSENRQTLLYGMG
                     DQHLEIAASKLAARYKVEITLETPKVAFRETIRKNSDVDMKYKKQTGGHGQYGHVKMK
                     FEPSHDLDTPYVFEECVVGGAVPKNYFPAVEKGLQESVLKGPLAGYPVVGVKATLYDG
                     SYHPVDSSEMAFKTATTQAFKKGFLEASPVLLEPIASLKVTVPDDYTGDVMGDLNKRR
                     GRVLGMTPIAGGKQVIEADIPMTGLFGYCTVLRSMTGGRGTYAYEFARYEQAPSDVQE
                     KEIAARAEE"
BASE COUNT          740 a          796 c          509 g          928 t
ORIGIN      
        1 ttctactttt tcgtccacaa gttggtacat cgtctctaca tttgcgctct tttgggagtt
       61 ctttctcgct ctgacgtaac ttaaaatcgc atctgccgtt gccgttttcg ccgaaacgat
      121 actgtcgata ccgaaatccg ctaccattct cgcccttctg tcttcattca ctttggcaat
      181 aatctttccc gcgccctggc ttttcgcata aagcgaaaga ataatgtttt cttcatcgat
      241 tcctgtcagc gcaacaaatg catctacatt ctccacgcct tcttcactta aaagatcatg
      301 atctgtcgca tctccgtgga tcactgttac tttcggaaga caatcgcata attcttcaca
      361 ccgattttcg tctttttcga tgatctttac atgcattccg atcttacaaa gtctcgccgc
      421 cagataatag gccacccggc ctcctccgca aataagaact ttcttgatct ttgtctgaag
      481 ctgacgtttt ccatgcattt tgaaaaacgc ttccatcgcc tcatgcgcac cggtaatatg
      541 aagtttatcc cctgcctgaa gtacatattc cccatccggt ataaatactt ttccctcatg
      601 ttctaccgcg caaacaagga ttttgatctg aaacttttta tagattgccg caagagacat
      661 tcctacaaga cgattgtcct ccgaaacagt aaactctatc aattcaactc ttccttttac
      721 aaacgtctca atcttactcg caaccggaaa cagaagcaga cgactgatat cattcgccac
      781 aatcaattcc ggattcaccg ccatactaag atgaagctct tcttttaaaa gaccgatctg
      841 tttaaaataa atcggattac gcacgcgggc gatcgtatga cgagctccga gctttcttgc
      901 aataagacag ctcaacatat tacattcatc tgccgatgta catgcgatca ccagatctgc
      961 atgcggcaca tccgccgctt tttgcacgtc ggcatccacc ccgtttccgt ggacgcagaa
     1021 aatatcaagt ctgtcgatag caaaccgaag ttttctttcg ttactgtcga tcatcacaat
     1081 gtcataatca tccgccgaaa gctgacgggc cagtttatat ccgactttcc catcacctat
     1141 aatcacaatc ttcattctct ttccctctaa ctgattctct taaatatcat tttataattt
     1201 taaattttat gtctgtattt cttgacagta gagcaattat agcaccttta cacataaagt
     1261 acaatgatct ttgcaaattc ttaacgaacc gccgtcataa gaaaagcaga ataccgcaca
     1321 ccataaaaga tgcgcggtat ttgatccttt ttataacaga aatacctgcc ggtccgcaaa
     1381 ctgagcatac cgttcttttc tgttcttttt tttctgtttt actcttccgc tcttgctgcg
     1441 atctctttct cctgtacatc agacggagcc tgctcatatc ttgcaaattc atatgcgtat
     1501 gttcctcttc cgcctgtcat ggagcgaagc accgtacaat acccgaacaa tcctgtcatc
     1561 ggaatgtccg cttcgatcac ttgctttcca cctgcgatcg gagtcatgcc aagcactcgt
     1621 ccgcgtctct tattcagatc tcccatcaca tctcccgtat aatcgtccgg aacagtcact
     1681 ttcaaagaag cgatcggctc aagaagtacc ggagatgcct caagaaatcc tttcttaaac
     1741 gcctgggttg tagctgtttt gaacgccatt tccgaagaat ctaccggatg ataagatccg
     1801 tcatacaaag tcgcttttac accgaccacc ggatatccgg caagaggccc tttaagaacc
     1861 gattcctgca atcctttttc tacagccggg aaatagtttt tcggaactgc tccgccgact
     1921 acgcactctt caaatacata cggtgtatca agatcgtgag acggttcaaa tttcatctta
     1981 acgtgtccgt actgtccgtg gccgccggtc tgtttcttat atttcatatc tacatccgaa
     2041 tttttgcgga tcgtttcgcg gaatgcaact ttcggtgtct caagcgtgat ctccacttta
     2101 tatctcgccg caagtttgct tgctgcgatc tcgagatgct gatctcccat tccatagaga
     2161 agcgtctgac ggttctcact gtcatttacc gacttcagag taacatcctc tgccgtcatt
     2221 tttgcaagcg cctgtgaaac cttatcttca tcgccctttg ttttcaccgt atacttcata
     2281 tatgtgtaag gaacagaata atctgttttc gcataagata ccggatttgc ctttgtagaa
     2341 agcgtatcgc ccgtgcgtgt actgccgagt tttgcgatag ccccgatatc tcctgcaaac
     2401 agttcggaaa cttccgccgg tttatttccg cacattgtgt aaatctttcc gggtttctct
     2461 tctgcgtctg cgtctgcatt atataaaata tcatcgcctt tcaacactcc ggagcatact
     2521 tttacgaatg aatattttcc aatgaacgga tcgaccatcg tcttaaatac atatgcggtc
     2581 tttgcttttg agaattcata attgccttca aaaatctcgt ttgttctgcg attaatacct
     2641 gcgcattttc ttttatccgg actcgggaag aatcttacaa tgtcagaaag cagattcgca
     2701 acgccttgcg cctgcacgtt tgatcccata gcgacaggaa cgatatctcc gtccatcact
     2761 tccgtacgca ttgcagagcg tatctcttct accgaaaatt cttcgccgtt gaaataacgc
     2821 tccataaact cttcacttgt ctctgcgaca gcttccataa gagagtctct caaaatctca
     2881 agattcggtt tacagtaatc aggaatctcg cactcttctc tttgcccgat ctccgtatat
     2941 cttcttcctg catttttaat aacattgata tat
//
LOCUS       DBMDXF010000222         2969 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7409_length_2969_cov_1.669107, whole genome shotgun sequence.
ACCESSION   DBMDXF010000222 DBMDXF010000000
VERSION     DBMDXF010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7409_length_2969_cov_1.669107"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="ACZPD4_07860"
     CDS             <1..470
                     /locus_tag="ACZPD4_07860"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR1379004.1"
                     /translation="TSRKYFEGRDVYIGAGTVKKKEEIDVLSDMGVPYILTPGFDEQI
                     VRYALEKKIEVLPGVLTPSEVQAAVNCGIHLAKLFPADAFSDRYIKALKGPFPEMDYV
                     AVGGVTPENAVNFYRLGYKGVAAGSNLVPKHAKRSDLKKIRETARKYVEVSQI"
     gene            467..1210
                     /locus_tag="ACZPD4_07865"
     CDS             467..1210
                     /locus_tag="ACZPD4_07865"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1379005.1"
                     /translation="MSNQVKDMTWREVQERLREFPVVIVPIGSTEQHGYHLPIGTDVY
                     LAEALAEKTAEKTGALVYPSIHFGYSWSWRDRIGTVTIRQDILREILKDVVRSVERYG
                     VKILIFMNGHEANRSVIKYAIREIQDETDVKVLGMFYPGMEEIYDKYMETPTWGGMFH
                     ACEFETSLMLAVKPETVHMERAVQEYPVRPTTYGMDNTSIGELSVSGTYGNPVPATEE
                     KGEMMLEQFTENISAVIEECVKKLVREET"
     gene            1482..2522
                     /gene="argC"
                     /locus_tag="ACZPD4_07870"
     CDS             1482..2522
                     /gene="argC"
                     /locus_tag="ACZPD4_07870"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1379006.1"
                     /translation="MIKVGIIGATGYAGGELVRILTGHREAEIKWYGSRSYIDKKYAD
                     VYQNMFQIIDAVCMDDNMEELASQVDVIFTATPQGLCASLVNEEILSKTKIIDLSADF
                     RLKDVKIYEEWYKLEHKSPQFIEEAVYGLCEVNREDVKKARLVANPGCYTTCSILTAY
                     PLAKEGLIDMDTLIIDAKSGTSGAGRGAKLPNLYCEVNENIKAYGVATHRHTPEIEEQ
                     LGYASGKKVVLNFTPHLVPMNRGILVTEYAKLTKEVSYEDVKAVYDKYYGGEKFVRVL
                     QKDVCPETKWVEGSNYVDIGFKIDPRTNRIIMMGAIDNLVKGAAGQAVQNMNLMFGLP
                     ESEGLELVPMFP"
     gene            2596..>2969
                     /locus_tag="ACZPD4_07875"
     CDS             2596..>2969
                     /locus_tag="ACZPD4_07875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1379007.1"
                     /translation="MVRIMTIEDYEGVYALWKKIKGFGIRSIDDSKEGVARFLKRNPT
                     TSVVAEKDGRIVGSILCGHDGRRGCLYHVCVDEDYRRHGIGKRMVVFAMKALKEEKIN
                     KVSLIAFTENDIGNAFWNTIGWT"
BASE COUNT         1000 a          423 c          796 g          750 t
ORIGIN      
        1 gaacaagcag aaagtatttt gaaggtcggg atgtttatat cggtgcagga acagttaaga
       61 aaaaagagga aattgatgta ctttcagata tgggagtgcc gtatattctg actcccggat
      121 ttgatgagca gattgttcga tatgcattgg aaaagaagat agaagtttta cccggagtgc
      181 tgacaccgtc tgaagttcag gcagcggtaa attgtggaat ccatcttgca aaattatttc
      241 ctgccgatgc gttttccgat cgttatatta aagcgttaaa aggtcctttc ccggagatgg
      301 attatgtggc ggttggcggg gtaacaccgg aaaatgcagt gaatttctat agattaggat
      361 ataaaggtgt ggcggccgga agtaatcttg ttccgaaaca tgcaaagagg agtgatttga
      421 aaaagatcag agaaacagcg cgaaagtatg tggaggtgtc ccaaatatga gcaatcaagt
      481 aaaagatatg acatggcggg aagtgcagga gcggttacgt gagtttccgg tagtaatcgt
      541 tcctattgga tcaacggaac agcatgggta tcatcttcct attggaacag atgtttattt
      601 ggcggaagca ttggctgaaa agacagcaga aaaaacggga gcgcttgtgt atccaagtat
      661 tcattttggg tattcttgga gttggcgtga cagaatcgga actgtaacga tccgacagga
      721 tattcttcgg gaaatattga aagatgttgt tcgttctgta gaaagatacg gggtaaaaat
      781 tcttattttt atgaacggac atgaggcgaa caggtcggtg ataaaatatg cgatacggga
      841 aatacaagat gaaacagatg tgaaagtgtt ggggatgttt tatccgggaa tggaagaaat
      901 ttatgataaa tatatggaaa ctccgacatg gggaggaatg tttcatgcct gcgagtttga
      961 aacgtcactt atgctggcag taaagccgga gacagttcat atggagcggg cagtgcagga
     1021 atatcctgtc agaccgacta cgtacggaat ggacaacaca tcgatcggag agttgagcgt
     1081 ttcgggaaca tacggaaatc cggtacctgc aacagaagaa aaaggagaga tgatgctaga
     1141 acagtttaca gaaaatatat ctgctgttat agaggaatgt gtgaagaaac tggttagaga
     1201 agaaacatag aaaaaaataa tagaaagaaa tataaaaata tcggaaatcg aaccgtaagc
     1261 tgaagtttaa ttcggagagc gtctcgattt ccgatatttt ttgtaagtgg atataagtta
     1321 aatacagatt aatgtatttg ataacagaaa aaacttgcat attatgcaag aatgatgtat
     1381 tattatgcaa aaagaatgca tattcatttt attttttgtg aagaaatatt gagacagggc
     1441 aggtttgtga aaagcaaaca gcagagagga gcgaatagat tatgataaaa gttggaatta
     1501 tcggtgcaac aggatatgcc ggaggtgaac tggtcagaat attgacaggg catagagagg
     1561 cggaaattaa atggtacggt tcgagaagtt acatagataa aaaatatgcg gacgtatatc
     1621 aaaatatgtt tcagatcata gatgcggtct gcatggatga taatatggaa gaactggcat
     1681 cacaggtaga tgtgattttt acggctacgc cgcagggatt atgtgcgtct cttgtaaacg
     1741 aagaaatttt gtcaaagaca aagattatag atttaagcgc ggatttccgt ctgaaagacg
     1801 taaaaatata cgaggaatgg tataagctcg aacataaatc cccacagttt attgaagaag
     1861 cggtgtacgg attatgtgaa gttaatcggg aagatgtgaa gaaggcccgt ctcgtggcga
     1921 atccgggatg ctatacgact tgttccattt tgacggcata tccgttggca aaagaagggc
     1981 tgatcgatat ggacaccttg atcatcgatg caaaatcagg aacgtcagga gcgggaagag
     2041 gagcaaaact gccgaatctt tactgcgaag tgaatgaaaa tattaaagca tacggagtgg
     2101 cgacacacag acatacgccc gaaatcgaag aacagcttgg atacgcatcc gggaaaaaag
     2161 tcgttcttaa ttttacgccg catttagttc cgatgaacag aggaatttta gttacagagt
     2221 atgcaaaact tacgaaagaa gtaagctatg aagacgtaaa ggctgtatat gataaatatt
     2281 atggcggtga aaaatttgtg cgtgtgcttc aaaaagacgt atgccctgaa acgaaatggg
     2341 tggaaggaag taattatgtc gatatcgggt ttaagatcga tccacggaca aatcggatta
     2401 ttatgatggg agcgatcgat aatctggtta aaggagcggc aggacaggcg gttcagaata
     2461 tgaatctgat gttcggactt ccggaaagtg aaggccttga gcttgttccg atgtttcctt
     2521 aagatgtata tcgtgcaaaa agcagcaggg cagaaagtaa aaccaaacgg gaaaaaacag
     2581 aggagatgat gcagtatggt acggatcatg acgatagaag attatgaggg ggtatatgcc
     2641 ctttggaaaa aaataaaagg atttggtatc cgaagtatcg atgattcaaa agaaggagtg
     2701 gcgcgctttt tgaaacgaaa cccgacgaca agtgtcgtgg cagaaaaaga cggtcgcatt
     2761 gtcggaagta ttttgtgcgg tcacgacgga agacgggggt gtttgtatca tgtctgtgta
     2821 gatgaagatt acagacgtca cggaatcgga aaaaggatgg ttgtttttgc gatgaaagcg
     2881 ctgaaagaag aaaagattaa taaagtatct ttgatcgcat ttacggaaaa tgatattgga
     2941 aatgcatttt ggaatacaat cggatggac
//
LOCUS       DBMDXF010000223         2965 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7420_length_2965_cov_1.850209, whole genome shotgun sequence.
ACCESSION   DBMDXF010000223 DBMDXF010000000
VERSION     DBMDXF010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7420_length_2965_cov_1.850209"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="ACZPD4_07880"
     CDS             complement(<1..827)
                     /locus_tag="ACZPD4_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR1379008.1"
                     /translation="MSEKKIPYKIYLEESEMPKTWYNVRADMKNKPAPLLNPETFKPM
                     TEEELSVVFCEELVKQELDNENRYIEIPEKIRDFYKMYRPAPLIRAYCLEEKLQTPAK
                     IYYKFEGNNTSGSHKLNSAIAQAYYAKDQGLKGVTTETGAGQWGTALSMACSYLDLDC
                     KVYMVKCSYEQKPFRREVMRTYGASVTPSPSETTEVGRKILAEHPGTSGSLGCAISEA
                     VEVATHTPGYRYVLGSVLNQVLLHQSVIGVESKIAMDKYGIKPDIIIGCAGGGSNLGG
                     "
     gene            complement(1416..1772)
                     /gene="rplT"
                     /locus_tag="ACZPD4_07885"
     CDS             complement(1416..1772)
                     /gene="rplT"
                     /locus_tag="ACZPD4_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1379009.1"
                     /translation="MARIKGGMNARKKHNRTLKLAKGYRGARSKQYRVAKQSVMRALT
                     SAYAGRKQRKRQMRQLWIARINAAARMNDLSYSKFMHGLKLANVDINRKMLAELAVND
                     KEGFATLAALAKEKLA"
     gene            complement(1857..2054)
                     /gene="rpmI"
                     /locus_tag="ACZPD4_07890"
     CDS             complement(1857..2054)
                     /gene="rpmI"
                     /locus_tag="ACZPD4_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1379010.1"
                     /translation="MPKIKTNRAVAKRFKKTGTGKLKRNKAYKSHILTKKSTKRKRNL
                     RQSTITDATNVKNMKKVLPYL"
     gene            complement(2113..2607)
                     /gene="infC"
                     /locus_tag="ACZPD4_07895"
     CDS             complement(2113..2607)
                     /gene="infC"
                     /locus_tag="ACZPD4_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1379011.1"
                     /translation="MINEQIRDREIRLIGEDGEQLGIMSAREAMKIAQEAELDLVKIA
                     PAAKPPVCKIIDYGKYKYEQARKEKEAKKKQKTVEVKEVRLSPNIDTNDLNTKINNAK
                     KFISKGNKVKVTLRFRGREMAHVQQSKHILDDFAETLADVAVVEKPAKMEGRAMSMVL
                     AEKR"
BASE COUNT          727 a          722 c          455 g         1061 t
ORIGIN      
        1 cctcccaagt tggatcctcc gcctgcacat ccgataataa tgtccggctt gatcccatac
       61 ttatccattg caatctttga ctcaaccccg atcactgact gatgaagaag aacttgattc
      121 aacacacttc caagcacata gcggtatccc ggcgtatgtg tagccacttc cacagcctcg
      181 gaaatggcac atccaaggct tcctgaagtt cccggatgct ctgccaaaat ttttcttccc
      241 acttctgttg tttcagacgg tgaaggagta acactcgctc cataagtacg cattacttct
      301 cttcggaatg gtttctgttc ataggaacat ttcaccatat acaccttaca gtccaaatca
      361 agataagaac atgccattga cagagcagtt ccccactgtc ccgctcccgt ctccgttgtc
      421 acgcctttca agccttggtc tttcgcataa tatgcctggg cgatcgccga attcagctta
      481 tggcttccac ttgtattatt cccttcaaat ttataataaa tttttgcagg tgtctgtaat
      541 ttttcctcaa gacaatacgc acgaatcagc ggcgccggac gatacatctt gtaaaagtca
      601 cgtatttttt ccggaatttc tatataacga ttttcattat ccaattcctg cttcactaat
      661 tcctcgcaaa atacaacgct taattcttct tctgtcatcg gcttaaatgt ttccggattc
      721 agaaggggcg ccggcttgtt tttcatatct gcccggacat tataccatgt cttcggcatc
      781 tcactttctt ccaaataaat tttataaggg atcttcttct cactcattgt tattctcctt
      841 tcaaaatttt ctttatatac ctctgaattt ttgtggaact ttaacttgcc cggatatgat
      901 tcctttacag acaattctca aaatatcttt tacatcataa attctttctt taataatata
      961 aaaaactcct gtcccacaca atgatactat tgcagggaca agagtcgtaa ttctaaatta
     1021 tatatcaaaa ctcctgcggt gccacccggc ttgatgtgaa aacacatcca ctcacgcata
     1081 ctaacatatg cccattttta taacggagtt atctctccgg ctcacctact tctatacttc
     1141 aaatgttcag cttgccctca gaagcccatt catcccaagt cagtataccg cattccacca
     1201 tctacggctc tctgtgaaac tgattatcgg aattacttgc tcttcctcat cggtttacag
     1261 ccactttatc acgttaaaat ataaatgtca atattttttt acttttcttt tcatcttttc
     1321 aaaaactttt caaaaactca tcaaaacaaa atatacttac aagaaagact cccaatcagt
     1381 ataactgttc gggagtcttt ttgttcttac ttttattatg caagtttttc ttttgcaaga
     1441 gctgcaagtg ttgcgaaacc ttctttatca tttactgcaa gctcagcaag catctttctg
     1501 ttaatatcaa cgtttgccag ttttaatccg tgcatgaatt tgctgtaaga cagatcgttc
     1561 attcttgccg ctgcattaat acgtgcaatc caaagctgtc tcatctgacg tttgcgctgc
     1621 tttcttcctg catatgcgga tgtaagtgct ctcattacag actgttttgc aactctgtac
     1681 tgtttagaac gagctcctct atatcctttt gccaatttca atgttctgtt atgtttcttc
     1741 ctagcgttca ttccgccttt gattcttgcc atttcttaat cctccttaaa taagaccatt
     1801 attctgtaat cactgtatcg tccgacaaca tctcatacgc acgatcagac ttcttcttac
     1861 agatatggta ataccttctt catgttcttt acgtttgttg catctgtaat tgtcgactgt
     1921 ctgagatttc tttttctctt tgtcgatttt tttgttaaga tatggctctt ataagcttta
     1981 tttcttttca gtttccctgt acctgtcttt ttgaagcgct ttgctactgc tctgtttgtt
     2041 ttgatttttg gcatgataat ttcctccttt attgtgctgc ctctttggat ctcattccgg
     2101 gcatatcggt tcttaacgtt tctcagctaa aaccatgctc atagctcttc cttccatttt
     2161 tgccggtttt tcaactaccg caacatctgc cagtgtctcg gcaaaatcat caaggatatg
     2221 cttactttgc tgaacgtgcg ccatctctct tcctctgaaa cgaagtgtca ctttcacttt
     2281 attgcctttg gaaatgaatt tctttgcatt gtttatcttc gtgttcaagt catttgtatc
     2341 aatgttcggt gagagacgta cttctttaac ctcgaccgtt ttctgcttct tcttcgcctc
     2401 tttttctttt cttgcctgct cgtatttata ctttccgtaa tcaataatct tgcagaccgg
     2461 cggtttagcc gcaggtgcga tcttcacgag atcaagttcc gcttcctgag cgattttcat
     2521 agcctcacgg gctgacatga tacctaactg ttccccatcc tcgccgatca gacgaatctc
     2581 tctgtctctg atctgctcgt taatcattaa atcgctaatt gtcgtatacc tccgtcaaat
     2641 gaattccttc aggtttccta tgcgaaacac aaaaaaacga gtgaatatca tatccacccg
     2701 acaatcaata aaaccatttc ggaaaatccg aaaggaagtt cttcgttatc agaccatagt
     2761 cacgcgcttg tgctatggtg agagtggata ctctactttg ttttttgctt cacgcaaaga
     2821 ttatactaac acacatactt tcctttgtca atctttattt ttcttaactg ttattaactt
     2881 gtgataatgt ctccttaaga ttcacttgag gttttgtctc tttttggtat tctattaaag
     2941 ctatgaaaga aggtaaaatt acatt
//
LOCUS       DBMDXF010000224         2959 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7444_length_2959_cov_1.335899, whole genome shotgun sequence.
ACCESSION   DBMDXF010000224 DBMDXF010000000
VERSION     DBMDXF010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7444_length_2959_cov_1.335899"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="ACZPD4_07900"
     CDS             complement(<1..1121)
                     /locus_tag="ACZPD4_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379012.1"
                     /translation="MKVLKKFASIIIAVCLMIPVLSTVVFAADGVLMFSDPSTKVGEN
                     VSVDFVVQSSGGTIGSVDVTMSYDPKALEFVSGDGFTADGAGTLTYKGTGGGTELRTA
                     VVFRALTVANTQISVSSSSATISSGDTLNLNNGSSAISIAAADDGTTSVEPTSAPNSP
                     AGESTDIVVTVDGNDYHFSEAFTTTDIPEGYSETKMTFNGEERKFVANDAGVYLGFLM
                     DDAGKGKFFLFNTEDATFAPFAQIAISDTTSLIVVDAPKSAKLPSSYQEVDMTVQEEN
                     FPAWSDPSNSRYYVIYALNTRTGEKSFYQYDTEDGTYQYFEAPKEETKKKDSESGIVA
                     KLGEFVAENTMVVLVAAAALFLLLLILAIVFAVKLVHRN"
     gene            complement(1170..2606)
                     /locus_tag="ACZPD4_07905"
     CDS             complement(1170..2606)
                     /locus_tag="ACZPD4_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1379013.1"
                     /translation="MHRGKETSTKPKKPVTRRKDFSASGKGKKRKKRRVHKMKPWQIA
                     LICVGAFIAALLAFGLMYVYAKWSKIDTKSIKADDIIINEEVKKNKNVDLGDGYTNVA
                     LFGVDSRDGDLGEGNRTDCIIVASLNNKTKEIKMVSVYRDTLLDLSEGTYQKCNAAYS
                     YGGPVLAINMLNMNLDLDIQDYVTVDFGAIADAVDLLGGIEIEVTEEELPYINQYIPE
                     TANSAGKAANLLQSAGLQKLDGTQATTYARIRSTAGGDFTRTERQRLVIEKMFEKAKG
                     ADIGTLNKIIDKVFPQVSTSFTLPEILKYASAYSEYKLVGNMGFPEDKYTDMLSEIGS
                     VVVPDNLVSNVTKLHEFLFGITGYVPSSTVQTVGSNISYTASAKKSSGTGSQSSYDSG
                     ESYNDSGYTDNSGNYEGGNTGGNYDDGSMSGNSGNTGNSGETGGVGGNGSTGDNTGGT
                     TVEPPVVPDGGTDSGGGQTEAPPEGTVQ"
BASE COUNT          757 a          774 c          478 g          950 t
ORIGIN      
        1 tgatttcgat ggacaagttt taccgcaaat acgattgcca aaatcaagag aagcaaaaac
       61 aatgctgccg ccgctacaag aacaaccatt gtattttccg ctacaaattc gcccagcttc
      121 gcaactatgc cgctctcact gtctttcttt ttcgtctcct ctttcggagc ctcaaaatac
      181 tgatatgttc cgtcttctgt atcgtactga tagaaagatt tttcacctgt ccttgtattc
      241 aatgcataaa taacataata tctggaattg gacggatcag accatgccgg gaagttttct
      301 tcctgaactg tcatgtccac ttcctgataa ctgctcggaa gttttgccga cttcggtgca
      361 tcaacaacga tcaaagatgt tgtatccgat attgcaattt gtgcgaaagg agcaaatgtt
      421 gcatcctctg tattaaacag gaaaaatttt ccttttcctg cgtcatccat taaaaatcca
      481 agataaactc ccgcgtcatt tgctacaaat tttctctctt ctccgttaaa tgtcatcttt
      541 gtctcagaat aaccttccgg aatatctgtt gtcgtaaacg cctccgaaaa atgataatca
      601 tttccgtcta cagtcacaac aatatctgta ctttcacctg ccggtgaatt aggagccgat
      661 gtcggttcta cacttgttgt tccatcgtct gccgctgcaa tcgaaattgc ggaagatcca
      721 ttgttcaaat ttaatgtatc tccggaagaa atcgtagctg agctgctgct tacagaaatc
      781 tgcgtattcg caaccgtaag agctctgaaa actaccgccg ttctcagctc tgttccgccg
      841 cctgtacctt tataagtcag cgtacccgca ccgtctgccg taaatccatc gccgctgaca
      901 aattcaagcg ccttcggatc ataactcatt gtaacatcca cacttccgat cgtaccgcct
      961 gaactttgaa caacaaaatc aacgctcaca ttctctccga ctttcgttga cggatcagaa
     1021 aacatcaata cgccatccgc cgcaaatacc accgtactca acaccggaat catcaaacat
     1081 accgcaataa taatgcttgc aaatttcttt aatactttca tatacttaac ctctttctgt
     1141 tttttatctt cccctgttcc ggttggatat tattgaactg taccttccgg aggtgcttcg
     1201 gtctgtccgc cgccgctgtc tgttccgcca tccggcacaa ccggaggttc tacagttgta
     1261 ccgcctgtat tatccccggt actgccattt cctccgacgc cgcctgtttc tcccgaattt
     1321 ccggtattac cactgtttcc gctcatgctt ccgtcatcat aatttccgcc ggtgtttccg
     1381 ccttcgtagt taccgctgtt atcggtatat ccactgtcat tataagattc accgctatca
     1441 tatgacgact gcgatcctgt tcccgaagac ttctttgcac ttgcagtata cgaaatatta
     1501 gaaccgaccg tctgcactgt agacgatgga acatatcctg taatgccaaa caggaattca
     1561 tgaagctttg ttacattcga tacaagatta tccggcacga caacgcttcc gatctctgaa
     1621 agcatatctg tatacttatc ttccgggaat cccatatttc ctaccagttt atattcactg
     1681 taagctgaag catatttcaa aatctccggc agcgtaaaac ttgttgaaac ttgcgggaac
     1741 actttatcga tgatcttgtt taacgttccg atatccgctc ccttcgcctt ttcaaacatt
     1801 ttttctatta caaggcgctg acgctccgta cgagtaaaat ctccgcctgc cgtagaacga
     1861 attctggcat aagttgtcgc ctgcgttccg tctaatttct gaaggcctgc actttgaaga
     1921 agatttgccg cctttcccgc cgaatttgcc gtttcaggaa tatattgatt aatatacgga
     1981 agttcttcct ctgtaacttc aatctctatg ccgcccaaca gatctaccgc atctgcgatc
     2041 gcgccaaaat caaccgtcac ataatcctga atatcaagat caagattcat gttcaacata
     2101 ttgatagcaa gtacgggacc tccgtagcta tatgccgcat tacacttctg ataagttcct
     2161 tctgaaagat caagcagcgt gtctctgtac actgacacca tcttgatttc ctttgtctta
     2221 ttattcaagc ttgcaacgat aatacaatcc gtacgatttc cttctcccaa gtcaccgtct
     2281 cttgaatcca cgccaaacag tgcaacattc gtataaccgt ctccaagatc tacattttta
     2341 tttttcttca cttcttcatt gataatgatg tcgtccgctt taattgattt cgtatcaatc
     2401 ttactccact tcgcataaac atacattaat ccgaatgcta aaagcgctgc gatgaaagct
     2461 cctacgcaga ttaatgcgat ctgccatggc ttcatcttat gaacacgtct ctttttgcgc
     2521 ttctttcctt ttcctgaagc ggaaaaatct ttcctgcggg taaccggttt ctttggctta
     2581 gttgacgtct ctttccccct gtgcattgct tgccttctgc gggatgaatt acgcttcacc
     2641 ttgttctttg acatattttc tcacctattt tagtatatac ttaatctaca ttataccatg
     2701 atacaacaaa tctctacatt attcaatcat ttcccctgaa aagattaaat aaaaatcccc
     2761 aaagttcccc ggggattttt atctgcatat tgaggctatt tcgcacaata tacttattca
     2821 tattttttta attgttcaag aatcgttctc gccttttctt cccatgtatt ctctaaagcc
     2881 tctttatcca ataacgcctt atatgtttca tctgttttca gcaatattgc cttatctaac
     2941 tgctctaaaa attctttat
//
LOCUS       DBMDXF010000225         2926 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7538_length_2926_cov_1.991504, whole genome shotgun sequence.
ACCESSION   DBMDXF010000225 DBMDXF010000000
VERSION     DBMDXF010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7538_length_2926_cov_1.991504"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="ACZPD4_07910"
     CDS             complement(<1..219)
                     /locus_tag="ACZPD4_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1379014.1"
                     /translation="MWYQKDWFTGSDYYNLKEKQIHAEEIEIKKPLEQKFHERTEIYY
                     VKSGDADIWINGDKYQMKEGVFCCLYIHH"
     gene            complement(324..1619)
                     /locus_tag="ACZPD4_07915"
     CDS             complement(324..1619)
                     /locus_tag="ACZPD4_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 domain-containing protein"
                     /protein_id="HIR1379015.1"
                     /translation="MDKTETNDLLEECMLYFKARPVYKKLFLKMRDKYAGLGHFGGTA
                     MLTSLSREEKSQLGGFFQRDYTSNKTITISADLMKKCLESSKFAGLTWELILETYFGE
                     PLQVKKEIELAESKRREDYFAEILESISDESGREWLRSILEEKKEGYLLITQLYKESP
                     EELRSILTYVTTGIAKLKVFQDKKQKELLAVFSANVTGNPHYFDEGKTGEKLLFNYLG
                     ERNFDLKQEGLSRAEYKNRIYYEAGILKDEVSNDALAYGIHGWKPDGGLHEGIEGFLE
                     NREPVKLTLQTIGRLEKVCGQSSQVYVVENPAVFSVLIREYPQRTVICGNGQLRLAVL
                     ILMDKFSCDTVFWYAGDFDPEGLLIAQKLKVRYGERLKLWKYEADLYETYLSEVELSD
                     RRMKKLEQVSVQELQEIREAMYKKRRSAYQESMMEALRK"
     gene            complement(1612..>2926)
                     /locus_tag="ACZPD4_07920"
     CDS             complement(1612..>2926)
                     /locus_tag="ACZPD4_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1379016.1"
                     /translation="LEDCIERLEKLPSEIEASVTQSSHLETKEKQFIEKLGDIEVSVG
                     KYRQQKDRFCTAFEMEYQLGYVECPFAVSDDAEERAEKICRMFMGRFGNKRHSEILEN
                     LQNVYHMNRGYLLEYQVTLQSLFEELDEESGVFDTSMRRIDISAKYRGITIKFKELIA
                     RMKEDAEVQRRLLSDKDRELFEDILANTISKKIRGKIQASKRWVEKMNTLMESMKTSS
                     GLTLSLRWKNKRAEKEEQLDTRELVELLQKDAEIMRPEEAQQLSGHFRSKIEEARKSA
                     NDLGNVQSFHAIMRNVLDYRQWFEFQLECQKTGEKKRELTDRVFFTFSGGEKAMSMYV
                     PLFSAVVAKYAGARQDAPYLISLDEAFAGVDEMNIRDMFRLMVEFDFNFMINSQILWG
                     DYDTVPALAIYQLVRPENAKYVTVIRYIWNGNIKSMVTEKDLSHG"
BASE COUNT          734 a          799 c          406 g          987 t
ORIGIN      
        1 atgatgtatg taaaggcagc agaaaacgcc ctctttcatt tgatatttat ctccgttgat
       61 ccaaatatcg gcgtcaccgc tcttaacata atatatctct gtcctttcat gaaatttttg
      121 ttcgagcggc tttttaatct cgatctcttc tgcatgaatc tgtttttctt ttaaattata
      181 gtaatcactt cctgtaaacc aatccttctg ataccacatt ttatgctcct caccgtttct
      241 tttttcagat ctggttttct tcgatcactc ctgatcgcat tttatattat aatcctttag
      301 cgtcagatga tcccaaacgg caattacttc ctcagcgctt ccatcatgct ttcctgatat
      361 gccgatcttc gtttcttata catcgcttcc cttatttcct gcagttcttg aacagacacc
      421 tgctccagct ttttcatccg cctgtcgctt agttctactt ccgataaata tgtttcatac
      481 aaatccgcct catacttcca aagtttcagc cgctctccat atcggacctt taatttctgt
      541 gcgatcaaaa gcccctccgg atcaaaatcc cccgcatacc aaaaaacagt gtcgcacgaa
      601 aatttatcca taagaataag taccgccaga cgtaattgcc cattcccgca gatcacagtc
      661 cgctgcggat actcccttat caatacagaa aatactgccg gattttccac aacgtatacc
      721 tgactactct gtccacagac cttctctaat cttccaatcg tctgtaacgt caattttaca
      781 ggctctctat tctcaagaaa accttcaatg ccttcatgta aaccgccgtc cggtttccat
      841 ccgtgaatcc catatgccag cgcatcattc gatacttcat ctttcaaaat ccctgcctca
      901 taatagattc tgtttttata ctctgctctg gaaagtcctt cttgttttaa atcgaaattc
      961 ctttctccaa gataattaaa gagtagtttt tcccctgtct tcccttcatc aaaataatgg
     1021 ggatttcctg tgacatttgc cgaaaacaca gctaacaatt ctttctgctt tttatcttga
     1081 aacactttca attttgcaat ccctgtcgtt acatacgtta gaatgctcct caattcctcc
     1141 gggctttctt tgtataactg cgtaataagc aaatatccct ctttcttttc ctccaatatg
     1201 ctgcgcagcc attctcgtcc gctttcatcc gaaatacttt ccagtatctc cgcaaaataa
     1261 tcttctcgcc ttttcgactc tgccagttcg atttcctttt ttacctgcag cggctctcca
     1321 aaataagttt ccagaatcaa ttcccaggtt aatcccgcaa atttactgga ttccaaacat
     1381 tttttcatta aatccgcaga aattgttatt gtcttattac ttgtataatc cctttgaaaa
     1441 aatcctccta attgactttt ctcttctctg ctcagcgacg taagcatcgc cgttccccca
     1501 aagtggccaa gaccggcata tttatcccgc attttcagaa atagtttttt atataccgga
     1561 cgcgccttga aatacagcat gcattcttct aacagatcgt ttgtttctgt tttatccatg
     1621 actcaagtcc ttttctgtta ccatactttt tatatttcca ttccagatat accgaataac
     1681 cgtaacatat ttcgcattct ccgggcgaac aagctgatag attgccaaag ccggaaccgt
     1741 atcataatct ccccagagaa tctgtgagtt gatcataaaa ttaaagtcaa actccaccat
     1801 cagccggaac atgtcccgaa tattcatttc atctactccc gcgaatgcct cgtccaagga
     1861 aatcaaatac ggagcatcct gtcttgcccc tgcatatttt gctactactg ccgaaaatag
     1921 cggcacgtac atcgacatcg ccttttctcc gccgctaaaa gtaaagaata cgcgatctgt
     1981 cagctccctt ttcttttctc ctgttttttg acattccaat tgaaactcaa accattgacg
     2041 ataatccagc acatttctca taatcgcatg aaaagactgc acatttccca gatcatttgc
     2101 agattttctt gcctcttcga tcttagagcg aaaatgtccg gataactgct gcgcctcttc
     2161 cggacgcata atttcggcat ctttttgcag aagttctaca agttccctcg tatccagctg
     2221 ttcttctttc tccgcccgct tatttttcca acgcagactc agcgtaagtc cgctggaagt
     2281 cttcatagac tccatcaatg tgttcatctt ctctacccat cgcttactgg cctggatttt
     2341 tccgcgaatt ttcttactga tcgtattggc aagaatatct tcaaataact ctctgtcctt
     2401 atcactcaaa agccggcgct gtacctctgc atcttccttc attctggcaa tcagttcttt
     2461 aaacttgatc gtgattcctc tatacttagc agaaatatcg attcgtctca tacttgtgtc
     2521 gaaaactccg ctctcctcat ccagttcttc aaataatgac tgcagcgtaa cctgatattc
     2581 caacagatat cccctattca tatgatatac attttgcaga ttctccaaaa tctcactgtg
     2641 ccttttattt ccgaatctcc ccataaacat cctgcatatc ttctccgccc gttcttccgc
     2701 atcgtcgctt accgcaaacg gacactctac atatccaagc tgatattcca tttcaaaagc
     2761 agtgcaaaat ctgtctttct gctgtctgta ctttccaact gaaacttcta tatctcccag
     2821 tttttctata aattgttttt cttttgtttc caaatgagag ctttgtgtaa cactagcctc
     2881 aatctctgac ggcagtttct ccaatcgctc tatgcaatct tccaat
//
LOCUS       DBMDXF010000226         2923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7544_length_2923_cov_1.556697, whole genome shotgun sequence.
ACCESSION   DBMDXF010000226 DBMDXF010000000
VERSION     DBMDXF010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7544_length_2923_cov_1.556697"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /gene="dnaG"
                     /locus_tag="ACZPD4_07925"
     CDS             <1..1384
                     /gene="dnaG"
                     /locus_tag="ACZPD4_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1379017.1"
                     /translation="ERGVGAHTYLTDRGLTEETITAFGLGYSTKYSDDLYQYLRGKGY
                     SEDLIRQAGLINTDERQGVYDKFWNRVIFPIMDVNSRVIGFGGRVMGDGKPKYLNSPE
                     TAVFDKSRNLYGLNRARTSRKTYFLLCEGYMDVISLHQAGFTNAVASLGTALTSGHAS
                     LIKRYVQEVYLTYDSDDAGTRAALRAVPILKEAGISAKVIRMDPYKDPDEFIKNLGAQ
                     EFENRIQNARNGFMFSLEILEREFDMASPEGKTAFFREAANRLLSFEDELERNNYIEA
                     VASAYKVSKESLEKLVAKTAVSTGLARPVTRPKKADGAVLQKEDGIVTSQKVLLTWMI
                     ENENLYSKISSYISPDDFTGELNRRVAKLLYEQHEKGELNPAKLLNHFTSEEEHREAA
                     SLFHTKIRQLKTKEEQDQALKDTILKVKAHSINERTLNLDPSDMAGLQHLMEEKRKLE
                     ELRQLHISID"
     gene            1441..2553
                     /gene="rpoD"
                     /locus_tag="ACZPD4_07930"
     CDS             1441..2553
                     /gene="rpoD"
                     /locus_tag="ACZPD4_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1379018.1"
                     /translation="MEENAQKFLEKMNELVALGKKKKSILDVQEINDFFADMELSAEQ
                     MEKVFEYLEGHNIDVLRITADTDDDVDIDNMIAEEEEVDMENIDLSVPEGVSIEDPVR
                     MYLKEIGKVPLLSAEREIELAKRMEEGDEDAKKELAEANLRLVVSIAKRYVGRGMLFL
                     DLIQEGNLGLIKAVEKFDYHKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETI
                     NKLIRVSRQLLQELGREPLPEEIAKELDMPVERVREILKISQEPVSLETPIGEEEDSH
                     LGDFIQDDNVPVPAEAAAQTLLKEQLDEVLDTLTEREQKVLRLRFGMNDGRARTLEEV
                     GKEFDVTRERIRQIEAKALRKLRHPSRSRKLRDYLD"
     gene            2593..>2923
                     /locus_tag="ACZPD4_07935"
     CDS             2593..>2923
                     /locus_tag="ACZPD4_07935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1379019.1"
                     /translation="MELSKRLRAVADLVTAGYQVADIGTDHAYIPIFLTETGKTDYAV
                     AMDVNKGPLRKAQENICAYKMEEQIETRLSDGFSALKVQEVQSAVIAGMGGNLVIRIL
                     EEGHDVVS"
BASE COUNT          970 a          485 c          771 g          697 t
ORIGIN      
        1 cgaaagagga gtgggagcac atacatattt gacggacaga ggtttgacag aagagacgat
       61 cacggcgttt ggacttggat attcaacaaa atacagtgat gatctgtatc aatatttgag
      121 aggaaaaggg tatagtgaag atctgatccg gcaggccgga ctgatcaata cagatgaacg
      181 acagggagtg tatgataaat tttggaatcg tgtcatcttt ccgatcatgg atgtaaacag
      241 ccgggtgatc gggttcggag gccgcgttat gggggacgga aaaccgaaat atttgaattc
      301 tcccgagacg gcagtgttcg acaaaagcag gaatttatat ggacttaaca gagcgaggac
      361 atcgagaaaa acttattttt tgctttgtga gggatatatg gatgttatct ctttgcatca
      421 ggctgggttt acaaatgccg tggcatctct cggaacggct cttacatcag gacatgcttc
      481 tttgatcaaa agatatgttc aggaagttta tctgacatat gacagtgatg atgcaggtac
      541 aagagcggcg cttcgagcag ttccgatact gaaagaggcg ggaatatcgg caaaagtaat
      601 ccgtatggat ccgtataaag atccggacga atttattaaa aatcttggcg cgcaggaatt
      661 tgaaaatcgt atccaaaatg caagaaacgg gtttatgttc agtcttgaaa tattagagag
      721 agaatttgat atggcttccc cggaaggaaa aactgctttt ttcagagagg cggcaaatag
      781 gctgctctca tttgaagatg aattggaacg gaacaattat atcgaggcag ttgcatctgc
      841 atataaagta agcaaagaga gtctggaaaa acttgtcgca aaaactgctg taagtacagg
      901 acttgcccgc ccggtcacac gcccgaaaaa agccgatggg gcggtcttgc aaaaagaaga
      961 cggaattgtt acatcgcaga aagttctgct tacatggatg atcgaaaatg aaaatctgta
     1021 ttcaaagata agcagttaca taagtccgga tgatttcaca ggagaattaa atcggcgcgt
     1081 tgcgaaactt ttatatgagc agcatgaaaa aggagaacta aatccggcaa aacttttgaa
     1141 tcatttcaca tcagaggaag aacataggga agcggcttct ctttttcata cgaaaatccg
     1201 ccaactgaaa acaaaagaag aacaggatca ggcgttgaaa gatacaattt taaaagtaaa
     1261 agctcacagc atcaatgagc gaacgctgaa tctggatccg tcagatatgg ccggattgca
     1321 gcatttaatg gaagaaaagc ggaagcttga ggaactgagg caactgcata tttctataga
     1381 ttaaggataa aataaaacaa gatgatctgc gtatgcgcac agatcaggaa ggatggacac
     1441 atggaagaga atgcacagaa atttctggaa aaaatgaacg agcttgtcgc tctgggaaag
     1501 aagaaaaaaa gtatacttga tgttcaggag atcaatgact tttttgcgga catggaatta
     1561 agcgccgaac agatggaaaa agtttttgag tatttagaag gacataatat tgacgtcttg
     1621 cggatcacgg ctgatacgga cgatgatgta gatatcgaca atatgatcgc agaggaagaa
     1681 gaagtcgata tggagaatat cgacctttcc gttccggaag gagtaagtat tgaagatccg
     1741 gttcgtatgt atttgaaaga aatcggaaaa gtaccgcttt taagtgcaga gcgagagatt
     1801 gaactggcga aacggatgga agaaggcgac gaggatgcaa aaaaagagct ggcagaagcg
     1861 aacctgcgtc ttgttgtcag cattgcgaag cggtatgtag gacgaggcat gctctttctt
     1921 gatctgatcc aggaaggaaa tctaggcttg atcaaagctg ttgaaaagtt tgattatcat
     1981 aaaggttata aattcagcac atatgcgaca tggtggattc gtcaggcgat cacaagagct
     2041 atagcggatc aggcaaggac aattagaatt ccggttcata tggtagagac gatcaataaa
     2101 ctgatccgtg tgtcaagaca gcttctgcag gaacttggaa gagaaccgct tccggaagaa
     2161 atcgccaaag agctggatat gcctgttgag cgtgttcggg agattttgaa gatttctcaa
     2221 gaaccggttt cgctcgaaac tccgatcggc gaggaagagg acagtcattt aggagatttt
     2281 attcaggatg ataacgtgcc tgtaccggca gaagctgcgg cgcagacgct cttaaaagaa
     2341 caacttgatg aagtgctcga tacactgacc gaaagggagc agaaagtatt acgtcttcgt
     2401 tttggaatga atgacggaag agcgcgtaca ttggaggaag tgggaaaaga atttgacgtt
     2461 acgagagagc gtatccgtca gattgaagca aaagctcttc ggaagctgcg ccatccaagt
     2521 cgaagcagaa aattaagaga ttatcttgat taattatccg gcaaacagtg taaccgagaa
     2581 aagaggatac agatggaatt atcaaaaaga ctccgtgcag ttgcagatct tgtaactgcc
     2641 ggatatcaag tggccgatat cggaacggat catgcgtaca taccgatttt tctgacagag
     2701 acaggaaaaa cagattatgc ggtagcaatg gatgtcaata aaggaccgct tagaaaagcg
     2761 caggaaaata tttgtgcgta taaaatggaa gaacagatag aaacacgtct ctcagatgga
     2821 ttttctgcat tgaaagtaca agaggtgcag tctgcagtaa ttgcggggat gggtggaaat
     2881 cttgtgattc gtattttgga ggaagggcat gatgtagttt cat
//
LOCUS       DBMDXF010000227         2910 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7576_length_2910_cov_1.851905, whole genome shotgun sequence.
ACCESSION   DBMDXF010000227 DBMDXF010000000
VERSION     DBMDXF010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7576_length_2910_cov_1.851905"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="ACZPD4_07940"
     CDS             <1..940
                     /locus_tag="ACZPD4_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1379020.1"
                     /translation="VSFPLGVMTCVTGVSGSGKSSLVNEILYKRLARDLNRARTIPGK
                     HKKIEGLDQVDKVIDIDQSPIGRTPRSNPATYTGVFDLIRDLFAATPDAKARGYKKGR
                     FSFNVKGGRCEACSGDGILKIEMHFLPDVYVPCEVCGGKRYNRETLEVQYKGKTIYDI
                     LNMTVEEALEFFAHVPSIRRKIETLNDVGLSYIRLGQPSTELSGGEAQRIKLATELSK
                     RSTGKTVYILDEPTTGLHFADVHKLTEILRRLTDDGNTVIVIEHNLDVIKTADYIIDI
                     GPEGGERGGTVVAHGTPEDVAENENSYTGKYIKKML"
     gene            1353..2342
                     /gene="mreB"
                     /locus_tag="ACZPD4_07945"
     CDS             1353..2342
                     /gene="mreB"
                     /locus_tag="ACZPD4_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1379021.1"
                     /translation="MSVDIGIDLGTASVLVYVRGKGVVLKEPSVVAFDRDTNVIKAIG
                     EEARLMLGRTPGNIVAVRPLRQGVISDYTVTEKMIKYFVRKSLGKRTFKKPRISICVP
                     SGVTEVEKKAVEEATYAAGAREVHLIEEPVAAAIGAGIDIAKPCGNMIVDIGGGTADI
                     AVISLGGTVVNTSIKVAGDDFDEAIVRYMRKKHNLLIGERTAEDIKIKIGTTYPLVEE
                     ETMEVRGRNLVTGLPKTVTVTSSETEEALREATGQIVEAVIAVLEQTPPELSADILDR
                     GIVLTGGGSMLRGLEELIEERTGINTMTAEDPMKVVAIGTGQFVEFMSGRGEV"
     gene            2468..>2910
                     /locus_tag="ACZPD4_07950"
     CDS             2468..>2910
                     /locus_tag="ACZPD4_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrrC family ATP-dependent DNA helicase"
                     /protein_id="HIR1379022.1"
                     /translation="MEKVTGYVGHIVFRNAENGYTVFHLENDDGEVTCVGNFNYITEG
                     ELLEITGEYVTHSVYGNQLKVMSHVVKEPEDLVSIERYLGSGAVKGIGAALAGRIVRK
                     FKEDTFRIIEEEPERLAEIKGISERKAREISSQLLSKYCLNPLSF"
BASE COUNT          895 a          451 c          825 g          739 t
ORIGIN      
        1 tgtgtcattt cctcttggag ttatgacttg tgttaccgga gtttcaggtt cgggaaaaag
       61 ctctcttgta aatgaaattt tgtataaaag gcttgcacgt gatttgaatc gcgcgagaac
      121 aattccggga aaacataaaa aaatagaagg gttagatcag gtagataagg tgattgatat
      181 cgatcagtcg ccgatcggac gaaccccaag gtcaaatccg gcaacctata caggtgtgtt
      241 tgatctgata agagatttgt ttgcggcaac tccggatgca aaagcgcgcg gatataagaa
      301 agggcggttt agttttaatg tgaaaggcgg acgctgcgag gcgtgctcgg gagacggcat
      361 tcttaaaatc gaaatgcatt ttttgcctga tgtatatgtt ccgtgtgaag tttgcggggg
      421 aaagcgatat aatcgagaga ctttggaagt acaatataaa gggaaaacga tttatgatat
      481 tctcaacatg acagtggaag aggcgttgga attttttgct catgttccga gtatcaggcg
      541 caagatagag acattgaacg atgtaggtct ttcttatatc cgtctcggac agccttctac
      601 agaattgtcc ggaggtgaag cacagcgcat taagctggca acggaactta gtaaacggag
      661 tacaggaaaa actgtgtata ttttggatga gccgacgaca ggacttcatt ttgcggatgt
      721 gcataaattg acggagatct tgcggcgtct gacagatgac ggaaatacgg ttattgtaat
      781 cgaacacaat ctggatgtta taaaaacagc agattacatt attgatatcg gtccggaagg
      841 cggagaacgc ggtggtacgg tcgttgcaca cggaacaccg gaagatgtgg cagagaatga
      901 aaattcctat acagggaagt atataaagaa gatgctgtag aaaacactgt agttataaaa
      961 aataacagtt cagtctgcgc cattcataaa atcacgctgt gtgcttattg tggccggcgc
     1021 cactgtttcg ctcataaatg tgcgcttgtc gcatacagat gtccgttagc gaaaacatga
     1081 gagcatgttt tgtgctttcc gtacactgta gggctgaact gttacaataa aaattataaa
     1141 aaagttgtaa aaagtatcat gtaattaaaa aaaagggttt tcattttttg ttgagtcgat
     1201 tatactaaag aatgcagatt ggatgcaggg gtatcatccg gtctggaaat atgtgaatga
     1261 taagacctgt ttggggagag atacagacat tttcgttttt gaggagatgt cagcgggaaa
     1321 agagtataaa gatttagaag ggagaatgga atatgtcggt agatatcgga atagatctgg
     1381 gaacagccag tgtgcttgtc tatgtccgcg gcaaaggagt cgttttgaaa gaaccatcag
     1441 ttgtcgcgtt tgacagagat acgaatgtaa tcaaagcgat cggggaagaa gcgagactga
     1501 tgctcggaag gacgcctggg aatatcgttg cggttcgtcc tctcaggcag ggcgtgattt
     1561 cggattatac agtgacagaa aagatgatca aatattttgt ccgaaagtcg cttggtaaga
     1621 gaacattcaa aaaaccgcgt attagtatct gtgtgcccag tggtgtcaca gaagtggaaa
     1681 agaaagcggt agaagaggct acttatgcgg caggtgcgcg ggaagtgcac ttgattgaag
     1741 agcctgtagc ggccgcgatt ggcgcgggaa ttgatattgc aaagccatgt gggaatatga
     1801 ttgtagatat cggcggaggt actgcggata ttgcggtaat ctcactgggc ggcactgttg
     1861 tgaatacatc gattaaggtg gcgggagatg attttgatga ggcaatcgtc cgttatatgc
     1921 gtaaaaaaca taatcttctg atcggtgaaa gaacggcgga agatataaag ataaagatag
     1981 gaacaacata tcctcttgta gaggaagaga ctatggaggt taggggaaga aaccttgtaa
     2041 caggattacc gaaaactgtt acggtaactt cttcggagac agaggaggct ttgagggaag
     2101 ccaccgggca gattgttgaa gctgtgatag cggtattgga gcagactccg ccggagttgt
     2161 cggcagatat acttgaccgt ggaatcgttt tgacaggcgg aggttctatg cttcgcggat
     2221 tagaggaatt gatcgaggag aggacaggca ttaacacgat gacagcggaa gatccgatga
     2281 aagttgttgc gatcggaaca ggacaatttg ttgaatttat gagcggacgg ggcgaagtct
     2341 gacgtgaaga gctgatttgg caaagagaga gaaggagcag ggatgtgagc aatacggcac
     2401 atccctgtta taatgtggat ataaagcgaa atattgtgga aaacaataac ggaaagagga
     2461 aagatctgtg gaaaaagtta cgggatatgt aggtcacatt gtgtttcgga atgcagaaaa
     2521 cggatataca gtgtttcatt tggaaaatga tgacggagaa gtgacctgtg tggggaattt
     2581 taattacatt acagaagggg aactgttgga aataacgggg gagtatgtga ctcatagtgt
     2641 gtatggaaat cagttgaagg taatgtctca cgttgtgaaa gaaccggaag atctcgtatc
     2701 catagaacgg tatcttggat cgggagccgt taaaggaatc ggggcggctc tggcgggacg
     2761 tattgtcagg aagtttaaag aagatacttt tcgtatcata gaagaagaac cggagcgtct
     2821 tgccgaaatc aaaggaatca gcgagcgtaa agcaagagag atttcatcac agttgttatc
     2881 aaaatattgc ttgaatccat tatcattcag
//
LOCUS       DBMDXF010000228         2907 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7585_length_2907_cov_1.997862, whole genome shotgun sequence.
ACCESSION   DBMDXF010000228 DBMDXF010000000
VERSION     DBMDXF010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7585_length_2907_cov_1.997862"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            21..1298
                     /locus_tag="ACZPD4_07955"
     CDS             21..1298
                     /locus_tag="ACZPD4_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HIR1379023.1"
                     /translation="MNSKTTSEKLIQFIENSPSAFHTVETIKFLLLKNNFSELCENKH
                     WHITAGGNYFVTRNNSSLIAFSIPENPLAGMRIIASHSDSPTFKIKENPEMECGHHYV
                     KLNVERYGGMIYAPWLDRPLSIAGRVIIKDKETNKLQSKLINIDRDLVLIPNLAIHMN
                     RDINSGYQYNPQKDLLPLYGNLSAKDTFMKQIADAAEVSEQEILGHDLFLYNRQKASI
                     WGASGEFLSAPRLDDLQCVFASVEGFLAGQKQTYMPILCVLDNEEVGSGTKQGAASTF
                     LYDTLIRVNSCLGLSEEDYLIRLADSFMISADNAHAIHPNHQDYSDPVNHPYLNGGIV
                     IKFNANQKYCTDGFSSAVFRDLCRTVNVPVQTFVNRSDMAGGSTLGNISNTQVAVDTV
                     DIGLPQLAMHSPYETAGVYDTDYFIKAAAEFFS"
     gene            1356..2345
                     /locus_tag="ACZPD4_07960"
     CDS             1356..2345
                     /locus_tag="ACZPD4_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1379024.1"
                     /translation="MTLKIGIIGTNFISDDFCDPAAQVDGIELYAVYSRSADTGNHFA
                     ERHNIPTVFTDYEQFLDSPIDAVYVASPNFAHCSQSIKAMEKKKHVLCEKVMASNEQE
                     ALSMIECAKKNHVVLLEAMRPDFDPAFDMIKNSLPRIGKIRRASFEFCQYSSRYDRFK
                     DGIIENAFNPALGNAAVSDIGVYCIHSLVRLFGTPKNIHSMSSSLENNFECSGIVLME
                     YPDMIAEAIYSKVTVSYRPSVIQGENGSILIDYISHPTQIELRLREGSRDPINGGIKE
                     ILPYSPVNNNMIYEIKNFLSLVENNGIEHEYLQYSLDTIRIIDHVLASHTSVH"
     gene            complement(2548..>2907)
                     /locus_tag="ACZPD4_07965"
     CDS             complement(2548..>2907)
                     /locus_tag="ACZPD4_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1379025.1"
                     /translation="TAKVDPTDAKDVTIIWTTSDETVLKVDEAGKVTALKVGKATITA
                     TAVQGEKKVKVTDSVEIEVEEETATPTPNPDPQPTPEKNDGAVQTGDNVSPMLYAGAA
                     MTAAAVLALIQRKRRKG"
BASE COUNT          886 a          634 c          516 g          871 t
ORIGIN      
        1 atataaaagg agccgatttt atgaattcaa aaacaacaag tgaaaagctt atccaattta
       61 ttgaaaacag tccatcagct tttcatacgg tagaaacaat taaatttctt cttttaaaaa
      121 ataatttctc ggaattgtgt gagaacaaac actggcacat aactgccgga ggaaattatt
      181 ttgtgacacg aaataattca tcactgatcg cattttccat tcccgaaaat ccacttgcag
      241 gaatgcgtat tatcgcaagc cacagtgatt caccgacttt taaaatcaaa gaaaatcccg
      301 aaatggaatg cggtcatcac tatgtcaaac taaatgtaga acgctatgga ggaatgattt
      361 acgcaccttg gctcgatcgt ccgctttcta ttgccggaag agtcattatt aaggataaag
      421 aaacaaataa attacaatcg aaattaataa acatcgacag agatcttgtt ttaattccaa
      481 atctcgcaat tcatatgaat agagatatta acagcggata tcaatataat ccacaaaaag
      541 atctgcttcc tctttacggc aatctttctg caaaagacac ttttatgaaa caaatcgccg
      601 atgcagccga agtgtctgaa caagagatcc tcggacatga cctcttcctt tataacagac
      661 aaaaagcaag tatatggggc gcttccggtg agtttttatc tgcgccgcgg cttgatgatc
      721 tgcaatgtgt gtttgcctct gttgaaggat ttcttgcagg acaaaaacaa acttatatgc
      781 cgattctctg tgttctcgat aacgaagaag tgggaagtgg aacaaagcaa ggcgctgcat
      841 ctacattttt gtatgatacg cttatccgag tcaactcttg tctcggactt tccgaagaag
      901 actaccttat tcggcttgca gacagcttta tgatttccgc agacaatgct catgcgatcc
      961 atccgaatca tcaagactac tctgatccgg tcaatcatcc gtatctaaac ggtgggatcg
     1021 tcattaaatt taacgcaaat caaaaatact gcacagacgg attttcttct gcggtatttc
     1081 gcgatctgtg ccgcacagtc aatgttcctg tccaaacatt tgtcaaccgt tccgacatgg
     1141 caggaggatc aactctcgga aatatatcta acacccaagt tgcagtcgac acagtcgata
     1201 tcgggctgcc tcagcttgcc atgcattctc cctacgagac ggcaggagta tacgataccg
     1261 actattttat caaagctgca gccgaatttt tcagttgagc aaagaaagaa atcaaaaaat
     1321 ataaaaatat tccaaaataa agaaggagga tacaaatgac tttaaaaata ggtattatcg
     1381 gaacaaactt tataagtgat gatttttgcg atcccgctgc gcaggttgac gggatagagc
     1441 tgtacgctgt atattccaga tccgctgaca caggaaatca ttttgccgaa agacataata
     1501 ttcccactgt attcactgac tatgaacagt ttcttgattc accgatcgac gcagtatacg
     1561 tggcttctcc taattttgca cactgctctc aatctataaa agcaatggaa aagaaaaagc
     1621 atgtactctg tgaaaaagta atggcatcaa atgaacaaga ggctctttcc atgatcgagt
     1681 gtgccaaaaa aaatcacgtt gtccttttag aagcaatgcg tcccgatttt gatcccgcat
     1741 ttgatatgat caaaaactcg cttccccgca tcggaaaaat tcgaagagca tcttttgaat
     1801 tttgccagta ttcaagccgc tatgatcgtt tcaaagacgg aattatagaa aatgcattta
     1861 atcctgcact cggaaacgcc gctgtatcag acatcggagt ttattgtatt cattcccttg
     1921 tccgcctttt cggtacacct aaaaacattc attctatgtc ttcctcattg gaaaacaatt
     1981 ttgaatgcag cggcattgtt ctgatggaat atcccgatat gatcgcagaa gcaatctatt
     2041 ccaaagttac ggtttcctat cgtccgagcg tcattcaagg agaaaacggt tctatcctga
     2101 tcgactacat aagtcatccg acacaaatag aactcagact gcgggaaggc tctcgtgatc
     2161 cgatcaacgg tggaatcaaa gaaatactgc cttattcacc tgtaaacaac aatatgattt
     2221 atgaaataaa aaattttctt tcacttgttg aaaacaatgg aatcgagcac gaatacctac
     2281 aatattcgct tgatacgatc cgcattatag atcacgtact tgcttcacat acttccgtac
     2341 attaaaaaat agatccatct attctactaa aaataaatcg atctattcca gtcatcttga
     2401 gttaaacttt aatacaacaa attaacgttg caaaaaagac tgtatatctt acaagaattt
     2461 cttctgtttg atatacagtc ttttctttta tcttagcact ttttctttgc ttttcctatc
     2521 cgcaatgtac aaaggataat gtactttcta tccttttctt ctctttctct gaatcaaagc
     2581 aagtactgcc gcggctgtca ttgctgctcc tgcatacagc atcggagaaa cattatctcc
     2641 tgtttgaact gctccgtcat tcttctccgg tgtcggctgt ggatctggat ttggagtagg
     2701 agtcgcagtt tcttcttcaa cttcaatttc cacactgtcc gtaactttaa ctttcttttc
     2761 accctgcact gccgttgccg tgatcgttgc ttttccgact ttcagtgctg ttacttttcc
     2821 tgcttcatct actttgagca ccgtctcatc agatgttgtc catataattg taacatcttt
     2881 tgcatctgtc ggatcaactt ttgctgt
//
LOCUS       DBMDXF010000229         2894 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7626_length_2894_cov_1.712137, whole genome shotgun sequence.
ACCESSION   DBMDXF010000229 DBMDXF010000000
VERSION     DBMDXF010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7626_length_2894_cov_1.712137"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(34..1122)
                     /locus_tag="ACZPD4_07970"
     CDS             complement(34..1122)
                     /locus_tag="ACZPD4_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1379026.1"
                     /translation="MFCENCGKEIKDENKFCPYCGASVQPEQNGEQPEQSAQPEQKSE
                     QPEQSAQPEQKNEQPKQSAQPEQKSEQPKQSAQSDQRLTQKETTGNSSADVKNKGVSH
                     PAPKKKKTAVVIGIIAMAAVIIIGIIAAVKMFGSSDAKETAKDSTYKEVKENAAEKES
                     KPAKKENVKPKKETDGEMKKDSGQEEKKEEKDNSKREAVLTDPVTMGWAGTYKDDDSG
                     DRLVIASIGYEWSYVIYTSSGQIFEKESECGAFGDYLEGQYYTFYKNENGLLSVTSGA
                     GKGWGNYRRISGTYTPDLEIEGTYENDTTTMIVYDQMAEASTKYVPTGSDIAAVRVEY
                     NNGLVIDGRLYTQGDGGLSIIDSVSILG"
     gene            complement(1152..1679)
                     /locus_tag="ACZPD4_07975"
     CDS             complement(1152..1679)
                     /locus_tag="ACZPD4_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1379027.1"
                     /translation="MFCEKCGKEIKDGDKFCPYCGAENEINKEEPSHDIPPDFSVKGA
                     GKKKKGAVIAIAVLVIIVAIGIFKCSSKDEILRYNVHVINNTGFDIYALYASEPDVDN
                     WEEDLLEYGIVYDGERVDIEFVITEEDLDWDFAIEDFEGNVLEFYGLSFAECDVEGAT
                     LILEYDGYKGTASLY"
     gene            complement(1717..2058)
                     /locus_tag="ACZPD4_07980"
     CDS             complement(1717..2058)
                     /locus_tag="ACZPD4_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR1379028.1"
                     /translation="MDKRKIYKKLMVIGRATGCHQLPERSFSFRGKQFPVCARCTGVF
                     IGEVLGIFLFKVGEISNIIAVVFSLIMFADWFVQYKFHYESNNIRRLITGLLCGYAFG
                     NIIMKYLRYFM"
     gene            complement(2066..>2894)
                     /locus_tag="ACZPD4_07985"
     CDS             complement(2066..>2894)
                     /locus_tag="ACZPD4_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR1379029.1"
                     /translation="MKYCRNCGKELNSDIQYCPNCGASVSEEQSGKNENSYQESNADG
                     SLWESLVEFFKALIEYMKEAGLHIIDAIDKIIQMPPNIPKDSICPYCNSEDTFPIVKN
                     ETEIKTKGYSMGRGCCGMCLLGPFGLLCGALFTGSKVKSRSITWWGCKNCGRQHLAQH
                     DAVETLSSLMDKMTVNCLCYGALGSLLLYPILDELVHGFLRTVIVVLIAVIVGVSLPL
                     YFCYQLCEDIDKQLGYSIWDILEVEKKQEIWSCIKFSMLSLAGTLIFVCPILVHFAE"
BASE COUNT          773 a          748 c          407 g          966 t
ORIGIN      
        1 agagaaagta cggcagattg cggcattggg gcatcatccc aatatggaaa cgctgtctat
       61 aatcgaaagc ccgccgtctc cctgcgtata taatcttccg tctataacca gcccgttatt
      121 atactctacc cggactgccg cgatatcaga tcctgtcgga acatactttg tacttgcctc
      181 tgccatttga tcgtacacga tcatcgtagt agtatcattt tcataagtac cttcaatctc
      241 cagatccggc gtatatgtac cgctgatcct gcgatagttt ccccagcctt tgcctgcacc
      301 tgaagttacg cttaacaatc cgttctcatt tttatagaac gtataatatt gcccttctaa
      361 ataatctccg aatgcaccac attctgattc tttctcaaaa atctgcccgc tcgacgtata
      421 gataacataa gaccattcat atccgattga tgcaatcaca agcctgtctc ccgaatcatc
      481 gtctttatat gtacctgccc atcccattgt gaccgggtct gtcaatacag cttccctttt
      541 actgttatct ttttcttcct ttttttcttc ttgcccggaa tcttttttca tttccccatc
      601 agtttctttt ttaggtttta cattttcctt tttcgccggc ttcgattctt tttcagctgc
      661 attctcttta acctctttat atgtcgaatc ttttgcagtt tctttcgcat cggaacttcc
      721 aaacattttc actgctgcaa taattccgat tattatgact gctgccatcg cgataattcc
      781 aattactacc gctgtttttt tctttttcgg cgcaggatga gacacaccct tgtttttaac
      841 atctgcactt gaattacctg ttgtctcttt ttgtgtcaac cgctggtctg actgtgccga
      901 ctgctttggt tgttcactct tctgttccgg ctgtgccgac tgctttggct gttcattttt
      961 ctgttccggc tgtgccgact gttctggctg ttcgctcttc tgttccggct gtgccgactg
     1021 ctctggctgt tcgccattct gttcaggctg tacagacgct ccgcaatacg gacagaactt
     1081 attctcatct tttatttctt ttccacaatt ttcacaaaac atttttttac atcctttatc
     1141 ttattttacc tttaatataa cgatgcagtt cccttataac cgtcatattc caaaatcaac
     1201 gtcgctcctt ctacatcaca ctctgcaaaa ctcaaaccat aaaattcaag aacatttcct
     1261 tcaaaatctt ctatcgcaaa atcccagtca agatcctcct ctgtgatcac aaactctata
     1321 tctacccttt caccatcata cactatacca tactcaagca aatcttcttc ccagttatcc
     1381 acatcaggtt ctgaagcata aagagcataa atatcaaaac cggtattatt aattacatgc
     1441 acattatatc ttaatatttc atcctttgaa ctgcatttaa aaataccgat cgccacaata
     1501 ataactaaaa cagcgatcgc gataaccgct ccttttttct tttttcctgc gcctttgacg
     1561 ctaaaatccg gcggtatatc atgactcggt tcttccttat ttatttcatt ttccgctccg
     1621 caatacggac aaaacttatc cccatctttt atttcttttc cacacttttc gcaaaacatg
     1681 gcaaattcct cctacattct cttttaatgt tttcaatcac ataaaatatc ttaaatattt
     1741 cataatgata tttccgaacg catatccgca cagaagaccg gttattaatc tccgtatatt
     1801 atttgattcg taatgaaatt tatattgtac gaaccaatct gcaaacatga tcagagaaaa
     1861 cacgaccgca attatattgg aaatctcgcc gaccttgaat aagaagattc ccaaaacttc
     1921 tcctataaac acccccgtac atcttgcgca taccggaaat tgttttccgc gaaaggaaaa
     1981 gcttcgttcc ggtaattgat gacatcccgt cgctcttcct atcaccataa gtttcttata
     2041 tatttttctt ttatccatca tcatcttact ctgcaaaatg tactaagatc ggacagacaa
     2101 aaatcagtgt accggctaaa gaaagcattg aaaattttat acaactccaa atttcctgtt
     2161 tcttttctac ttctaaaata tcccaaatac tataccctaa ctgtttgtct atatcctcgc
     2221 ataactgata gcaaaaatat aacggcaagg aaactcccac tatgacagcg atcaaaacaa
     2281 cgatgaccgt tcttaaaaat ccatgcacta attcatccag tatcgggtaa agcaaaagag
     2341 aacctagcgc gccatagcaa agacaattta ctgtcatttt atccatcaac gaacttaacg
     2401 tttcaacagc gtcatgctgc gctaaatgtt gtcttccgca atttttacat ccccaccagg
     2461 tgatagatct gctctttact ttagagcccg tgaagagcgc tccgcacaat aatccaaacg
     2521 gaccgagcaa acacattcca cagcacccgc gccccatact gtatcctttt gttttaatct
     2581 ctgtctcgtt cttaacaatc ggaaacgtat cctcgctgtt acaatacggg catatagaat
     2641 ctttcggtat attcggcggc atctgaatga ttttgtcaat cgcatcgata atatgaagcc
     2701 ccgcttcttt catatattca atcagcgctt taaaaaattc gacaagagat tcccaaagcg
     2761 atccatccgc gttactttcc tgatatgaat tctcattttt ccctgattgt tcctcactta
     2821 cagaagcgcc gcaattcgga caatattgaa tatctgaatt taattctttt ccacaatttc
     2881 tgcaatattt catc
//
LOCUS       DBMDXF010000230         2885 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7649_length_2885_cov_1.646193, whole genome shotgun sequence.
ACCESSION   DBMDXF010000230 DBMDXF010000000
VERSION     DBMDXF010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_2885_cov_1.646193"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="ACZPD4_07990"
     CDS             complement(<1..181)
                     /locus_tag="ACZPD4_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1379030.1"
                     /translation="MIENGMRYLRGYVKIQIQGYSPERFLNLCSYHHILIWGLAYEDH
                     CYELCMSVRDFKRIRP"
     gene            complement(178..465)
                     /locus_tag="ACZPD4_07995"
     CDS             complement(178..465)
                     /locus_tag="ACZPD4_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1379031.1"
                     /translation="MEKEQIKKKLASAASMPKDVIMGASVVTVLGMSEVCIENYSGIT
                     EYTDTLVRIRTKHGQIQLKGSCLEIEFYTNDEMKITGSINLIEFQEGRNLR"
     gene            complement(553..849)
                     /locus_tag="ACZPD4_08000"
     CDS             complement(553..849)
                     /locus_tag="ACZPD4_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR1379032.1"
                     /translation="MVHHIVMWKFKPEIEENKKEELKKAMEENLTALVGKIPGLLTLE
                     FVACPFGSSTHDMALVSTFEKEEDISVYAKHPAHVAVADTYVRPFVTERACLDY"
     gene            complement(883..2265)
                     /locus_tag="ACZPD4_08005"
     CDS             complement(883..2265)
                     /locus_tag="ACZPD4_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1379033.1"
                     /translation="MEQDKAFLGKEPIGKLLLKLALPTVAAQMINMLYNLVDRIYIGH
                     IPNVGVSALAGVGVCMPLIMLVSAFAALVGYGGSPRVSIYMGKKDNETAEKILGNCFA
                     MQIIISAILTAVLLIWSRDLLMLFGASRKSIEYAVSYMNIYAVGTIFVELTLGMNAFI
                     TAQGFAKTGMLSVLIGAISNIILDPIFIFGFGMGVKGAALATIISQALSCIWVISFLM
                     GRKTMLKIRKENLLLRPKIIFPCLALGAATFVMQASESVISVCFNASLQSYGGDMAVS
                     AMAILTSVMQFALLPLQGLGQGAQPIISYNYGAGNAGRVKSAFKLLLKVSLCYSTILW
                     GAVMLFPDVFAGMFTTEADLIVFTRPALRIYMAVMLIFGIQMACQMTFNALGRAVESI
                     VVAVMRKFILLLPLIYIMPLLFSSNKTMAVYLAEPAADLLAVTFTAILFTVRFKKALQ
                     TMENKKIQDT"
     gene            complement(2580..>2885)
                     /locus_tag="ACZPD4_08010"
     CDS             complement(2580..>2885)
                     /locus_tag="ACZPD4_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR1379034.1"
                     /translation="FNGVNLKQVTVYGNRGTYAEEFAKKNGCTFVADTSDYKKGDVDL
                     DGNVAIGDVRVVLRSICQKIELNEMQELAADVEKDDKVDIKDLRKILRYVCGKIDSL"
BASE COUNT          839 a          726 c          446 g          874 t
ORIGIN      
        1 aaggtctgat ccttttaaaa tctcgtacac tcatacaaag ttcatagcag tgatcttcat
       61 aggccaatcc ccagattaaa atatggtgat aggaacacag attcaaaaaa cgctcaggag
      121 aatatccctg aatctgtatt tttacatacc cccgcagata tcgcattcca ttttcaatca
      181 tcggagattc ctcccctcct gaaattcaat caaattgatc gaaccggtaa tcttcatttc
      241 gtcattcgta taaaactcta tttccaaaca acttcctttt aactgtatct gtccgtgttt
      301 tgttcttata cgtacgagag tgtctgtata ctccgtgatc ccgctgtaat tttcaataca
      361 cacttccgac attcccagaa ctgtaacaac cgaagctccc ataataacat ctttcggcat
      421 acttgcagcc gatgccaatt tctttttaat ctgttctttt tccatataaa aaatgccaca
      481 catacgcctt ttataacaag tatatgtgcg gcactttctg tcctattctt ctttatttcc
      541 gttgatcggc aatcaataat cgagacatgc tctctctgtc acaaacggcc ttacatatgt
      601 atcggcaaca gctacatgag ccgggtgttt tgcgtaaaca gaaatatctt cttccttctc
      661 aaacgtactg actaaagcca tatcatgagt gctcgatccg aacggacaag caacaaactc
      721 aagagtcaaa agtccgggga tttttcctac aagagctgta agattttctt ccatagcttt
      781 ttttaattct tcttttttat tttcttctat ttccggtttg aatttccaca taacaatatg
      841 atgtaccatt tttctacctc tttcttaata ttttcctgct ttttatgtat cttgtatttt
      901 tttgttctcc attgtttgca gcgctttctt gaatcgtact gtaaacaaga tcgccgtaaa
      961 cgtcacagcc agcagatctg ccgccggttc tgcaaggtaa acagccatcg ttttatttga
     1021 cgaaaacagc agcggcatta tgtaaatcag tggaaggagc aaaataaact tacgcatcac
     1081 ggcaaccaca atagactcta cagctcttcc gagtgcatta aacgtcatct gacacgccat
     1141 ctgaattccg aaaatcagca taaccgccat ataaatacga agcgccggac gggtaaatac
     1201 gatcaaatcg gcttctgtcg taaacattcc tgcaaacaca tccgggaaaa gcataaccgc
     1261 tccccataaa attgtcgaat aacaaagact tacttttaac aagagcttaa acgcactttt
     1321 tacacgccct gcattccccg ctccgtaatt ataactgatg atcggctgcg caccctgtcc
     1381 gaggccttga aggggaagca gtgcaaactg cataacactt gtcaaaatcg ccatcgcgct
     1441 tactgccata tctcctccgt aactttgtaa agaagcgtta aagcaaacag aaatcacact
     1501 ttcgctcgcc tgcataacga acgtcgccgc tcccaatgcc agacacggaa aaataatttt
     1561 cgggcgaagg agcaaatttt cttttcggat cttcagcatc gttttccttc ccattaggaa
     1621 agagattacc caaatacagg acaatgcctg cgatataatc gtggcaagag ccgctccttt
     1681 gacacccatt ccgaatccaa atataaagat cggatcaagt atgatattgg aaatcgcacc
     1741 gatcaataca gacaacattc ccgtctttgc gaatccctgc gctgtaataa acgcattcat
     1801 gccaagtgta agttctacaa agatcgtacc gactgcataa atgttcatat agctgactgc
     1861 atactcgatt gattttctgc tcgcgccaaa cagcataagc agatctctgc tccatataag
     1921 cagcactgct gtcaggattg cagatattat tatctgcatt gcaaaacagt ttcccaatat
     1981 cttttcggct gtttcattgt ctttctttcc catataaatc gacacccgcg gagacccgcc
     2041 atagccgacg agggccgcaa acgccgaaac aagcataatc aaaggcatac atactccgac
     2101 tcccgcgaga gcggaaactc ctacatttgg gatatgtccg atataaatcc tgtcaacaag
     2161 attatacagc atattgatca tttgcgccgc aactgtcgga agcgccagtt ttaaaagcag
     2221 ttttccgatc ggttcttttc ctaaaaacgc tttatcctgt tccatttttt agctcctgtc
     2281 ttttccgtta tttgttttta accataaaga acattataat tcttaaaatt caagtgtcaa
     2341 gctaaaaaat atcatataca catctacaaa aaatatcata tacacatcta cacatcattt
     2401 ccatatgaaa acaattacaa aaatttaatc cccgctccgt ctttgatatg ctccccctcc
     2461 tcgataaaca gttttattat tttaactgtc tacctagaag gggacatatc actttcaacg
     2521 aaacggggat tttttctgcc tcttatgttt cacttatgaa atctgattca cgatatcttt
     2581 tataacgaat caattttacc acacacatat cttaagattt tccgcagatc ttttatatct
     2641 accttatcgt ctttttccac atcagccgca agctcctgca tctcgttcaa ttctatcttc
     2701 tgacatatgc tgcgcaatac tacacgaaca tctccgatcg cgacatttcc atccaagtcc
     2761 acatcacctt ttttgtagtc ggaagtatct gccacaaaag tgcatccgtt tttcttcgca
     2821 aactcttctg catatgtacc tctgtttccg taaaccgtaa cctgcttcaa attcactccg
     2881 ttaaa
//
LOCUS       DBMDXF010000231         2861 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7734_length_2861_cov_1.458333, whole genome shotgun sequence.
ACCESSION   DBMDXF010000231 DBMDXF010000000
VERSION     DBMDXF010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_2861_cov_1.458333"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="ACZPD4_08015"
     CDS             <1..1456
                     /locus_tag="ACZPD4_08015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HIR1379035.1"
                     /translation="AIFSVLRAALRYAEQACNHFIAFKLLALIRDKVFQALRKLCPAK
                     LESKDKGDLISVITSDIELLEVFYAHTISPVLIWFFFTLIMTAFIASYHIWLGGLALI
                     AYLTIGVIIPLIISKISGDDGMKFRSKSGALSGFVLDSLRGLSETIQYGQGQKRLAEM
                     NKKTDELSADEERMKKIAGRNIAVTNTVILIFDFMMLFLSAYLYQTNAIGFDGILICT
                     LALFSSFGPAIALANLGSTLQNTFASGSRVLDILEEEPLVEEIKDEKEVSFNGAKAEN
                     VTFAYKDEIILDNLSADIPKNSVVGIVGRSGSGKSTLLKLFMRFWKVENGSVKLSDTD
                     IEHINTANLRNMESFVTQETHLFHDSIKNNIRIAKLNATDEEIKAACQKASVHEFIMT
                     LPQGYDTQVGELGDTLSGGERQRIGLARAFLHDAPFMLLDEPTSNLDSLNEAVILRSL
                     HEERNGKTVVLVSHRESTMRIADKIYSVEHGRMS"
     gene            1523..1921
                     /locus_tag="ACZPD4_08020"
     CDS             1523..1921
                     /locus_tag="ACZPD4_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR1379036.1"
                     /translation="MNIKKILYIIFGCISFGLGAVGAVLPVLPTFPFLIFAAFCFARS
                     SKKLDKWFKGTKLYKNNLETYAKGQGMTRKTKVRIMITVTFLMSFGFFIMFRKNLYIP
                     CSILFSVWLFHILYFTFGVKRYIPKENEGI"
     gene            1918..2274
                     /locus_tag="ACZPD4_08025"
     CDS             1918..2274
                     /locus_tag="ACZPD4_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme transporter CcmB"
                     /protein_id="HIR1379037.1"
                     /translation="MKRILNTLLFLTFILTLLVPITGVHIHKLASVIFLILCLVHTGV
                     YWKKMNIFRFFVLGLIFEVFLTGLFGMIFKQYPIILSIHTISSIAVVFFLAIHIFVFK
                     KKICYSLRSHAHNANK"
BASE COUNT          865 a          459 c          644 g          893 t
ORIGIN      
        1 ggcgatattc tcggtccttc gtgcggcgct tcgttatgca gagcaggcgt gcaatcattt
       61 tatcgcattt aaacttttgg ctctcatacg agataaagta tttcaagctc ttagaaagct
      121 atgtcccgcc aaattagaaa gcaaagataa aggggatttg atttcagtca ttacatcaga
      181 tatcgaactt ttggaagtat tctatgctca taccatttct ccggtattga tctggttttt
      241 ctttactttg atcatgacgg cttttattgc gtcgtaccat atttggcttg gcgggctggc
      301 tctcatcgcc tacctgacga tcggagtgat cattcctttg attatttcta agatcagcgg
      361 tgacgatgga atgaagtttc gcagcaagtc cggcgcactt tccggttttg tattagatag
      421 tttacgtgga ctttctgaga cgatccagta tggtcaggga caaaaacgtc ttgcagaaat
      481 gaataaaaaa acagatgaac tttccgcaga tgaagagaga atgaagaaaa ttgccggtcg
      541 aaatatagcg gttacaaata cggtaatctt aatatttgac tttatgatgc tttttctttc
      601 cgcatattta tatcagacaa acgccatagg atttgacggg atattgatct gtacgcttgc
      661 actgttttca tctttcggac cggcaatagc gcttgctaat ttaggcagca ctttgcaaaa
      721 cactttcgca tccggcagcc gcgtacttga tattttagag gaagagcctc ttgtagaaga
      781 gattaaagat gaaaaagagg tttcgtttaa cggtgccaag gcggaaaacg taacttttgc
      841 ttataaggac gaaataattc ttgacaacct ttcagcagat attccgaaaa attcagttgt
      901 tgggatcgtg ggcagaagcg gtagcggtaa atccactctt cttaaactat ttatgcgatt
      961 ttggaaggtg gagaatggaa gcgtcaaact ttccgatact gatatagagc atatcaatac
     1021 tgcaaatctt agaaatatgg aaagttttgt gacacaggaa acacatttgt ttcacgacag
     1081 cataaaaaat aatataagaa ttgcaaaact aaatgcaaca gatgaagaaa taaaggctgc
     1141 ttgtcaaaaa gcttctgttc atgagtttat tatgactttg ccgcaaggtt atgatacaca
     1201 ggtaggagag ctgggtgata cgctttcagg cggtgaaaga cagcggatcg gtcttgcccg
     1261 tgctttttta cacgacgcgc cgtttatgct acttgacgag cctacaagta atttagatag
     1321 tttaaatgaa gctgtcatac ttagatcttt acatgaagaa cggaatggaa aaacggttgt
     1381 gctcgtatca cacagagagt ctacgatgcg aattgccgac aagatatatt cggtagagca
     1441 tgggagaatg agctgataag aaaaatgtca tgtaaaccat actgttgaaa caaaaaaaga
     1501 aaaaagagat aggagaaaga agatgaatat aaaaaagata ctttacatta tttttgggtg
     1561 tataagcttt ggactgggcg cagtgggcgc agtgctaccg gttcttccga cgtttccgtt
     1621 tttgattttt gccgcttttt gttttgcgag aagttctaag aagttagaca aatggtttaa
     1681 gggaacaaaa ttatataaaa ataatcttga aacttatgca aaaggtcagg ggatgacgag
     1741 gaaaacaaaa gttcggatca tgatcacagt gacattttta atgtcttttg gcttttttat
     1801 aatgtttaga aaaaaccttt atataccttg ttcgatactt ttcagtgtct ggctgtttca
     1861 tattttgtat tttacgtttg gtgtaaaaag atatattccg aaggagaatg aagggatatg
     1921 aaaaggattt taaatacact tttgtttctt acatttattt tgacattgct tgtgccgatc
     1981 accggggtac atattcataa gctggcatcc gtaatttttc taatactttg tcttgtacat
     2041 acaggagttt attggaagaa gatgaatatt ttcaggtttt ttgtgcttgg cttaatattt
     2101 gaggtgtttc tgacaggact gttcggcatg atatttaaac aatatccgat aattttgtca
     2161 attcatacga tcagttctat tgcagttgtt ttctttttgg caatacatat ttttgtattt
     2221 aagaaaaaga tttgttatag tttaagatca catgcgcata atgcgaataa ataaaaaaaa
     2281 ggaatcatcg gaaaagaaga aaaggcttaa aattgattct tgacatttat aaagtcgggt
     2341 ggtatactgt gaaaaattta ataatatatt aaaccgttga agcggagata acgtcaatga
     2401 tgcctttaca gagagtccgg gtagctgaga gccggatgag aaacaagttg gcaaatggac
     2461 cgctgagggt gcagtcaaat ggatgttttc cagagtattc tgcaacgtgt gggcatacgt
     2521 cagttgccgg ggatatgctg gtatcctgct aagtgcacag gtcattctgt gaattcaagg
     2581 tggcaccgcg gatagtgtcg tacttttact gatgtacgta attcgtcctt gacagagatt
     2641 tatttctgtc aggggcgttt ttattttccg gaatatcaga taaatcccgg actgctctgg
     2701 gcagattgca gcgctgaaaa cataatgaat ttttaaagtg gaggtacaga tcatgaatat
     2761 tttaccgact ttggcagatg taagggaaat tgcttcagat ggagcataca aggtaatacc
     2821 ggtcagctgt gagattctat ctgattttat aacgcctgtg g
//
LOCUS       DBMDXF010000232         2836 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7798_length_2836_cov_1.184644, whole genome shotgun sequence.
ACCESSION   DBMDXF010000232 DBMDXF010000000
VERSION     DBMDXF010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7798_length_2836_cov_1.184644"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="ACZPD4_08030"
     CDS             <1..762
                     /locus_tag="ACZPD4_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1379038.1"
                     /translation="DSVEDQIIQINTQTGEVSCVLDLGELFSDYKEECTENEDGELDW
                     IHINTIQWIGDDAVLLSSRETSTILMIEDIYDTPQITYMIGEESFWEGTGYEELLLEK
                     DESAGTFSNTGGQHSVTYVQDDSLNAGEYYLYLFNNNFGVSKSKPAYDWEQIEGIETS
                     MKEGEESYYYKYKVNENNGTYSLIQSFKVPYSAYVSSAQEYKGNIIIDSGMQGIFGEY
                     DASGNIIQEFEMELADEYIYRVYKYDFADFYFEKQ"
     gene            848..2617
                     /locus_tag="ACZPD4_08035"
     CDS             848..2617
                     /locus_tag="ACZPD4_08035"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1379039.1"
                     /translation="MKKEKKKSVLTRIIPYAGKRKYMLFLAMAFSALSGILLLMPMWY
                     IHNIIKNVILNGNVNYTMIRENIAYAVIFPCVGLGLYVLAGIFSHLFAFEVEENIIKI
                     SVKKLLDKPLGYFMNKESGKLRGIIINGAGETHNVLAHQLPDIASTFVSPVVILVFLF
                     LFDWRLGLTSLIPVFIGLIFMATMLTAQGKKNREEYYKQINTLSAETVEYVRGIPVVK
                     TFGQSVESFKRLYTSIIVMKDSVLKMTMGYRNKMSMFEATSGSTAFFLVPAALFIIAA
                     GENPHEVVADSIIYFLIGPIFGVLIMRTATISQYLYFAELALDKIEDILEYDEMAYGK
                     EDQTTGGIEFKNVSFSYGSEKVLDNISFKVNRGETVALVGRSGSGKTTIARLAARFYD
                     ADEGEVLLGGSNIKEYDKTSLMRKISFVFQNARLFKMSLRENLLCGKEDAIDKEIEQA
                     LFDAGAKEIVDGLKDGLETVYGTKGTYFSGGEAQRLVIARAFLKDAEYVILDEATAFA
                     DPENERIIQESFKKLAENKTTLLIAHRLSTVVNADKILVIEKGRIAEEGKHEALLEKN
                     GIYKSLWDEYRRSVNWKIGGKND"
BASE COUNT          933 a          411 c          654 g          838 t
ORIGIN      
        1 gacagtgtgg aagatcaaat tattcagatt aatacgcaga caggtgaagt tagctgtgtg
       61 ctcgatttgg gagagttatt ttctgattat aaagaagaat gtacggaaaa tgaagatgga
      121 gaattggatt ggatacatat aaacacgata cagtggatag gggacgatgc cgttttactg
      181 agctccagag agacttcgac tattttgatg atcgaagata tttatgatac tcctcagata
      241 acatatatga ttggggagga gagtttctgg gaaggaacag gatatgagga attgttgctt
      301 gaaaaagatg agagtgcggg aacattttcc aatacgggag ggcagcattc tgtcacatat
      361 gtacaagatg attctttaaa tgcaggagaa tattatctct atctgtttaa taataatttc
      421 ggagtaagta agtcgaaacc tgcttatgat tgggaacaga tagaaggaat cgaaacatcg
      481 atgaaagaag gagaggaatc ctattattat aaatataaag taaatgaaaa taatggaacc
      541 tattccctga tacaatcttt taaagttcca tattctgctt atgtcagcag tgcacaggag
      601 tataagggaa atattattat agattccggt atgcagggaa ttttcggaga atatgacgca
      661 tcggggaata tcattcaaga atttgagatg gaacttgccg atgaatatat ttaccgtgta
      721 tataagtatg attttgcgga cttctatttt gaaaagcagt agaaatatat ggcgggattt
      781 taagtaaaat taaggtgagc agtatatgat ctgtctgtaa aatataaaac atatggagag
      841 gattaggatg aaaaaagaaa agaaaaagtc tgttttaaca agaatcatac cttatgcagg
      901 gaaaagaaaa tacatgcttt ttttggcaat ggctttttcg gctttgtcgg gaatcctgtt
      961 gctgatgcct atgtggtata ttcacaatat tataaaaaat gtgatcttga atggaaatgt
     1021 aaattacacc atgataagag aaaatatagc atatgcggta atatttcctt gtgtaggact
     1081 tggtttatat gttttggcag gaattttttc acaccttttt gcatttgaag ttgaagaaaa
     1141 tattataaag atcagtgtga aaaaactgtt agataaaccg ttggggtatt ttatgaacaa
     1201 agaaagcggg aaactgcgcg ggatcattat aaacggcgca ggtgaaacgc acaatgtctt
     1261 ggcgcatcag ctcccggaca ttgcctcgac atttgtttct ccggttgtca tattagtctt
     1321 tttgttttta tttgactgga ggttaggatt gacaagtctt atccctgttt ttatcggcct
     1381 tatttttatg gcgacaatgc tgactgctca agggaagaaa aacagagaag aatactataa
     1441 acagatcaat acattgtctg ccgagacagt tgaatatgtc agaggaatcc cggtagtaaa
     1501 aacattcggt cagagcgtgg aaagttttaa gcgtctttat acttctatta ttgttatgaa
     1561 ggacagcgtc ttgaaaatga ctatgggata cagaaataaa atgtctatgt ttgaagcgac
     1621 ttcgggttcg acggcgtttt ttcttgttcc ggctgctttg tttattattg cagcgggaga
     1681 aaatccgcat gaagttgtcg ctgattcgat catatacttt ttgatcggac cgattttcgg
     1741 agttcttatt atgcgtactg cgacaatcag tcaatatctt tattttgcag aactggctct
     1801 tgataaaata gaagatattc ttgagtatga tgagatggct tacgggaaag aagatcagac
     1861 gacaggcggg atcgaattta aaaacgtttc tttttcttac ggaagtgaaa aagtattaga
     1921 taacatttct ttcaaagtaa atcgaggtga aacagtggcg ctggtcggac gttcgggaag
     1981 cggaaagacg acaatcgccc gtcttgccgc aagattttac gatgcagatg aaggggaagt
     2041 tttattgggc ggaagcaata taaaagaata tgataaaact tcgcttatgc ggaaaatttc
     2101 atttgtgttt caaaatgcaa ggctttttaa aatgagcctg agagaaaatc ttctgtgtgg
     2161 gaaggaagat gccattgata aagagattga acaggcgctt tttgatgcgg gagcaaaaga
     2221 gattgtggac ggtctaaaag atggattgga gacagtgtat ggaacgaaag gtacatattt
     2281 ttcaggagga gaagcgcagc ggctcgtgat cgccagagct tttttaaagg atgcggaata
     2341 tgtgatactg gatgaagcca ccgcttttgc agatcctgaa aatgaacgca ttatacagga
     2401 atctttcaaa aagttagctg aaaataaaac gacattgctg attgcacatc ggctttcgac
     2461 agttgtaaat gcagacaaaa ttctcgtaat cgaaaagggg cgtattgcag aagaaggaaa
     2521 acacgaagcg cttttagaaa aaaacggaat atacaagtcg ctttgggacg aataccggag
     2581 atctgtcaac tggaagattg gaggtaaaaa tgattaatta ttttatgaaa aaatatgcga
     2641 ttcctgcccc ttcacatttc gaaatatcag tatgatggaa attgagcata acttttttta
     2701 atgtccttaa caaggggaac ttttctgcta aaaaggctgc tgctccacaa atgattactc
     2761 atctatagag cagcagcctt aacaccaaaa atattccctt tatttttcca tcatcatcac
     2821 actttcagca ataata
//
LOCUS       DBMDXF010000233         2800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7889_length_2800_cov_3.637644, whole genome shotgun sequence.
ACCESSION   DBMDXF010000233 DBMDXF010000000
VERSION     DBMDXF010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_2800_cov_3.637644"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="ACZPD4_08040"
     rRNA            complement(<1..419)
                     /locus_tag="ACZPD4_08040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1288..2313)
                     /locus_tag="ACZPD4_08045"
     CDS             complement(1288..2313)
                     /locus_tag="ACZPD4_08045"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1379040.1"
                     /translation="MEKKNLDWANIGFEYVQTDKRYVANYKDGKWDEGTLTEDANIVM
                     NECAGVLQYAQTVFEGMKAYTTEDGRIVTFRPDLNAARMVDSAKRLEMPVFPEDRFVD
                     AIVQTVQANAAYVPPYGSGASLYIRPYMFGINPVIGVKPADEYQFRVFTTPVGPYFKG
                     GAKPITICVSDFDRAAPHGTGHIKAGLNYAMSLHAIVTAHANGFDENMYLDSATRTKV
                     EETGGANFIFVTKDNKIVTPHSDTILPSITRRSIMYVAEHYLGLEVEERDVYFDEVKD
                     FAECGLCGTAAVISPVGKVVDHGNEICFPSGMEKMGPITQKLYETLTGIQMGHIEAPE
                     GWLKVIE"
     gene            complement(2394..>2800)
                     /locus_tag="ACZPD4_08050"
     CDS             complement(2394..>2800)
                     /locus_tag="ACZPD4_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR1379041.1"
                     /translation="CIVVVVLLFWMCIVFLRMDFTPIRLVADGTEVRITSGYTNTTMK
                     KKEIRSVKLLENGLPEDYYNRTNGSSDEKQLLGKFEGKKTGKCRMYIYVECTPILQIN
                     TSDEIIFINSKEKGAAEKWYEKIVQEKNRVHS"
BASE COUNT          698 a          705 c          459 g          938 t
ORIGIN      
        1 acgcggcgtc gctgcatcag ggtttccccc attgtgcaat attccccact gctgcctccc
       61 gtaggagttt gggccgtgtc tcagtcccaa tgtggccggt caccctctca ggtcggctac
      121 tgatcgtcgc cttggtaagc cgttacctta ccaaccagct aatcagacgc gggtccatct
      181 cataccaccg gagtttttac ccccgcacca tgcggtgctg tggtcttatg cggtattagc
      241 agtcatttct aactgttatc cccctgtatg aggcaggtta cccacgcgtt actcacccgt
      301 ccgccgctca gtcacaatcc tcttcatccg aagaatctaa gcaaagtgct tcgctcgact
      361 tgcatgtgtt aggcacgccg ccagcgttca tcctgagcca ggatcaaact ctcgttaaaa
      421 gtgtttgtct tggtcaagaa tcactactag caattctttc cctctttact gttttggcat
      481 tgaacactta atgagtctct cacgaattta gtgtgaaagc cgttcggcga agccgaacct
      541 catcttgtat caatgtagtt cttaaaaatt tttctcttta aagaaatttt caagggttgt
      601 tgtctattgt tcagttatca aggttctttc tctgttctgt ctcaagcgac agcttttata
      661 ttttatcaaa agctttatcg tttgtcaaga acttttttat ttttttgctg atcgctcaag
      721 cgacagcttt aatagattac cacatcgccg tgatattgtc aacaactttt ttactttctg
      781 atcttccgcc tccgcgtctt cagataagtt tttctgttgt ctcccgctat ccgatcgttt
      841 acagttccca ttctttggct gttccgcctc cgcgtctcca aaagtctgtt cctgtatttc
      901 gtatagccgt catcagcgac aagtgatatc ctatcatgta tggaagtaat tgtcaatgtt
      961 tttcttgttt tttatttatt tcagacaatt caaacaatta ccctttttac cgcttctttt
     1021 cagtcatagc tgctgcccta aaaacaagcg aatcaccgga agtgccaaaa ggcatcttct
     1081 ggaattataa gcagttacca agttctcgga cgagcccgga gggttttgtt ctgtaggaaa
     1141 tcgaaatttc ctatagcata aaaaacagcc cgcttcaggg attcccggtc gcggaactgt
     1201 ttttatattt caatcattac ttatattact tttctttata ctgtcttcta tactattttc
     1261 aaattgcttt ttatactgca ctttctgtca ttcgattact ttcagccatc cttccggtgc
     1321 ctcgatatgt cccatctgga ttcctgtcaa cgtctcatac aatttctgtg tgattggtcc
     1381 cattttctcc attccgcttg ggaaacagat ttcgttacca tgatctacca cttttccgac
     1441 aggcgaaatt actgctgccg ttccgcacag accacactct gcaaaatctt ttacttcatc
     1501 aaaataaacg tctctttctt ccacttcaag accaagataa tgttctgcta catacataat
     1561 agatcttctt gtaatagacg gaagaatcgt atctgaatga ggtgttacaa ttttattatc
     1621 ttttgttacg aaaataaagt ttgcgcctcc cgtttcttcc acttttgttc tcgttgccga
     1681 atcaagatac atgttttcat caaatccgtt tgcatgagcc gtaacaatcg catgaagact
     1741 cattgcataa ttaagccccg ccttaatatg ccccgttccg tgaggagctg cccggtcaaa
     1801 atcagataca cagatcgtaa tcggttttgc accaccttta aaatacggac ctacaggtgt
     1861 tgtaaacact ctgaactgat attcatctgc aggctttact ccgattacag gattaatccc
     1921 aaacatatat ggtctgatat ataatgaagc gcctgaacca taaggcggaa cataagcggc
     1981 atttgcctga accgtctgca cgattgcatc aacaaatcgg tcttccggaa agaccggcat
     2041 ttccaaacgt tttgccgaat ccaccatgcg cgccgcattc agatccgggc gaaaggtaac
     2101 gatacgtcca tcttctgtcg tatacgcttt cattccctca aatacagtct gtgcatattg
     2161 aagcactccg gcacattcat tcattacaat gttagcatcc tctgtaagcg ttccctcatc
     2221 ccattttccg tctttataat ttgcaacata tctcttatct gtctgtacat attcaaatcc
     2281 tatatttgcc cagtcaagat ttttcttttc cataataaag cgcctccatt cctccatttt
     2341 agaaaacaat cacttctttc tttaagacat tataataccg ccgtttttaa aaatcaagag
     2401 tgcactctgt ttttttcttg tacaatcttc tcataccatt tctctgccgc gcccttttct
     2461 ttactattaa taaatatgat ttcatctgaa gtatttatct gcaaaatcgg tgtacactct
     2521 acataaatat acattctgca ttttccagtc ttctttcctt caaattttcc cagcagctgt
     2581 ttctcgtctg aagatccatt tgtccggttg taataatcct ccggcagtcc gttctctaat
     2641 aattttacac tccgaatctc ttttttcttc atagttgtat tcgtatatcc ggaagtaatt
     2701 ctgacttccg ttccatctgc tacaagacga atcggtgtaa aatccattct taaaaagaca
     2761 atacacatcc agaacaaaag tactacaact acaatacaaa
//
LOCUS       DBMDXF010000234         2800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7890_length_2800_cov_2.286773, whole genome shotgun sequence.
ACCESSION   DBMDXF010000234 DBMDXF010000000
VERSION     DBMDXF010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7890_length_2800_cov_2.286773"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="ACZPD4_08055"
     CDS             <1..837
                     /locus_tag="ACZPD4_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR1379042.1"
                     /translation="NEADQRHHYANTLTNGSKNKTVEIRVGENVSGFSMELWTDIPNT
                     SSISIISPSGEDTSRIPFHAGSSAELDFLFERTKVSVDYRILVEKTTSELVFFRFNSP
                     AAGIWKIVIEPITVTDGQFHMWLPVTEFIDGEVYFLESDPYYTLTNPANTQSPVVVSY
                     YNGTTNAVSQTSGRGYTRSGQIKPDLTAPGINIRGPVSNGRFETHSGSCAACAVTAGA
                     VALMLEWLLYIQKAPGIDSFQIRSLLILGALRPDTMEYPNRIWGYGQLNLYHTFEVMR
                     KL"
     gene            complement(848..2104)
                     /locus_tag="ACZPD4_08060"
     CDS             complement(848..2104)
                     /locus_tag="ACZPD4_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HIR1379043.1"
                     /translation="MKKITKRILIASAVTAGTGVVFMGVGIALGGWPGVVFTKGGIHS
                     PYEQKTPYRQEKKEIEPFSELSLYVGSEADVVVMASKDEKYYVEYTLDGNYGKPKCEL
                     TNGKLSIAQQDHNGYMTGMFGLNLGENLSGEKSYITVYIPKGTLLETADIYNDYGNVN
                     WSNVNGKKVSIEADSGNVKIEQSETTSMKLTLNDGDAFADKLKAETFTLQSDYGDSTL
                     SNVSGKTFTVQSECGDVKTEQSETTSMELILNDGDVSVDGLKAETFVLQSEYGDSTIS
                     DVSGKVFTVQSECGEVELDRVLFEKMKVEALDGDVLSSEISCSFADVTLEYGDLRWDA
                     QKLENLTCQAECGDVDLILPEELEKYEVDLQIEYGDLSLPKDTPHDQYREEDGEVSYR
                     ISASEKNAGKKISVINEEGDVKVRYR"
     gene            complement(2088..2684)
                     /locus_tag="ACZPD4_08065"
     CDS             complement(2088..2684)
                     /locus_tag="ACZPD4_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1379044.1"
                     /translation="MTKSEYMKKLSYSLRRLPKEDFNQAIDYFEEYFAEAGEENEQQA
                     IEDLGSPEDAAKELIMNLAEKNMEQPPKTVKHSFKAVWIGILGICAAPIALPIALSLI
                     CVIAAVIVSVFCVFAGLFLTAVTLAGAAVIGVIGGAALLIQSLPDGLATLGFSLCCGA
                     VGIMSVYGIFCFLKWLLYKVAQLLGKIMKGGKKNEKNN"
BASE COUNT          720 a          734 c          505 g          841 t
ORIGIN      
        1 aatgaggcgg atcagcgtca tcactatgcc aatactttaa cgaacggatc aaaaaacaaa
       61 acggtagaaa tccgcgtcgg agaaaatgtt tcgggatttt ccatggaatt atggacagat
      121 attccgaata catcgtccat atccattata tccccttccg gggaagacac ctcgcgaatc
      181 ccttttcatg cgggatcaag cgcagagctt gattttctct ttgagcgtac aaaggtatcg
      241 gtcgattacc gtatcctcgt agaaaaaacc acttccgaac ttgtattttt ccgttttaac
      301 agcccggcgg caggcatttg gaaaatcgtc attgaaccga tcaccgtcac cgacggtcag
      361 tttcacatgt ggcttcctgt cacggaattt attgacggag aagtgtattt tcttgaatct
      421 gacccctatt atactttaac caatcccgca aatactcaaa gtcccgttgt cgtttcctat
      481 tataacggaa cgacaaacgc cgtatctcaa acatcaggca gaggatatac acgatccggt
      541 cagatcaagc cggacttaac tgctcccggg attaatatac gcggtcctgt ttccaacgga
      601 cgatttgaaa cacattccgg ctcctgcgca gcctgtgccg tcactgccgg cgctgtggcg
      661 ttgatgttgg aatggcttct ctatattcaa aaagctccgg gtatagattc cttccaaatc
      721 agaagtcttc tcatactcgg agctctgcgt cccgatacga tggagtatcc taatcgtata
      781 tggggatacg ggcagttaaa tctttaccat acatttgaag tgatgcgaaa attataacga
      841 tttaaattta gcgatagcga acctttacat cgccctcctc atttatgacg gagatcttct
      901 ttcctgcatt tttctctgat gccgatatgc ggtaagatac ttctccgtct tcctctctat
      961 actgatcatg gggagtgtct ttgggcagag aaagatctcc gtattcaatc tgcagatcaa
     1021 cttcatactt ttccagttct tcgggaagaa taagatctac gtctccgcac tcggcctggc
     1081 aggtcaaatt ttccagtttt tgcgcatccc agcgaagatc cccgtattct aatgtcacat
     1141 ctgcaaaaga acaggaaatt tcactcgata atacgtctcc atccagcgct tccactttca
     1201 tcttttcaaa caaaacccgg tctaattcca cttctccgca ttccgactgt accgtaaaca
     1261 ccttaccgga aacatcggaa atcgtgctgt cgccatactc cgattgtaat acaaacgttt
     1321 ccgctttcag tccatctaca gatacatctc cgtcatttaa aatcagttcc atcgacgttg
     1381 tttccgactg ctctgttttt acatctccgc attctgactg cactgtaaac gtcttaccgg
     1441 aaacattgga aagtgtactg tcgccataat ccgattgcag cgtaaatgtt tccgctttca
     1501 gtttgtctgc gaatgcatct ccgtcgttca aagtcagttt cattgacgtt gtttccgact
     1561 gttctatttt tacatttccg ctgtccgcct cgatcgacac tttttttcca ttgacatttg
     1621 accagttgac attcccataa tcattgtata tatctgcggt ttcaagaagc gttcctttcg
     1681 gaatataaac tgtaatgtaa gatttttctc ctgatagatt ttctccgagg ttcaatccga
     1741 acatacctgt catatatccg ttgtgatctt gctgagcaat agaaagtttt ccatttgtta
     1801 attcacattt aggtttaccg taatttccgt caagtgtata ttcgacataa tatttctcat
     1861 cttttgaagc cataacaact acatccgctt cgcttcccac atagagactc agttctgaaa
     1921 aaggttcgat ctctttcttt tcctgccgat aaggagtttt ctgctcgtac ggtgaatgga
     1981 ttccgccttt tgtaaaaaca actccgggcc agccgcctaa tgcgattcca actcccataa
     2041 agacaactcc tgttccggct gtcactgccg aagcaatcaa aatccgctta gttatttttt
     2101 tcatttttct ttcctccctt cataatcttt cctaaaagct gagccacttt ataaagaagc
     2161 catttcaaga aacagaaaat tccatataca gacataattc ctactgcgcc gcagcaaaga
     2221 ctgaatccta atgtcgccaa tccatccggg agactttgga tcagcagagc cgctcctcct
     2281 atcacgccga tcaccgcagc tcccgccagc gtaaccgccg taagaaacaa gcctgcaaac
     2341 acacagaaaa cagaaacaat cacggctgcg atcacacaga tcaaagaaag tgcgatcgga
     2401 agtgcgatcg gcgccgcgca gattccgaga ataccgatcc agactgcctt aaaactgtgt
     2461 tttaccgttt tcggcggctg ctccatattc ttttccgcca gattcataat caattctttt
     2521 gccgcatctt ccggcgatcc aagatcttcg atcgcctgct gttcattctc ctctcccgcc
     2581 tctgcaaagt attcctcaaa gtagtctatc gcctgattga aatcttcttt cggcagcctt
     2641 ctgagactgt atgacaattt tttcatatat tcactttttg tcatgataaa ttctctccct
     2701 ttacaatatc atccacaacc gtccgataac gctcccattc ctctgtcaat tctgccagat
     2761 actgtctccc ggcatctgtg atcgtataat acttcctgtt
//
LOCUS       DBMDXF010000235         2765 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_7996_length_2765_cov_1.445946, whole genome shotgun sequence.
ACCESSION   DBMDXF010000235 DBMDXF010000000
VERSION     DBMDXF010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7996_length_2765_cov_1.445946"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="cobA"
                     /locus_tag="ACZPD4_08070"
     CDS             complement(<1..1524)
                     /gene="cobA"
                     /locus_tag="ACZPD4_08070"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR1379045.1"
                     /translation="MEQSLYGKVWIAGAGPGDAGLLTLRTAELIEEADVIVYDALLSA
                     EILSRIPRETETIYVGKHAGNHPVPQEEINQILVREAKKGKRVLRLKGGDPFVFGRGG
                     EEIEILRNEKISFEIIPGITSSVAVPAYAGIPVTHRDYTSSFHVITGHTRRDVKPDID
                     YEALVKLNATLIFLMGVSAMETILTELIKAGMPEDMPAAVIERGTTARQRQVCATVKT
                     LKQQADEAKIKAPAIIVVGKVCSLSEEFHWIKDRILGGKQFLVTRPRQNSSALAKRLR
                     NLGAQVIEMPSIHTVAIDPNERLKKALGEIQHSEKEEWFVFTSPIGVHVFFEQLEKEA
                     WDMRRLLAGKAQIKIAAIGSATAAALKEHGLFADIVPKIYNAGELGKTLAENISEYSA
                     VTIFRAEEGSLELLPPLMETGVPVNDIALYRTEYEVSSLLRHKIEKMFQKEEIDAVTF
                     TSASTVKGFVKAIKNVELQNVSAVCIGEQTAAEARKYGMKIQVAKRADMDAMTEKIVE
                     "
     gene            complement(1530..2453)
                     /gene="hemC"
                     /locus_tag="ACZPD4_08075"
     CDS             complement(1530..2453)
                     /gene="hemC"
                     /locus_tag="ACZPD4_08075"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR1379046.1"
                     /translation="MQSVIRIGSRESRLAVKQAEIIKDQIRRCDQTILVEIITMKTTG
                     DKILDRSLESVGGKGLFVKELDQALADGRIDLAVHSLKDMPMEESSEFPILAYSRRED
                     PRDVLIYKSEKKEQGTEEGSILTKTGAVIGTSSKRRMIQIQKLYPNAVFQGIRGNIQT
                     RLSKLNKEEYDAAVLAAAGVKRLNMEAVIGRYFSVDEVIPAAGQGILAVQGRKEFLKD
                     TVWRQYVDDKKSRVQALAERSFIRVLDGGCTSPSAAYAKVKGNELELTGLYYNEKSGQ
                     YFVKKAASYIEDASSLGERLALTMKKQFGER"
     gene            complement(2432..2722)
                     /locus_tag="ACZPD4_08080"
     CDS             complement(2432..2722)
                     /locus_tag="ACZPD4_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379047.1"
                     /translation="MEEEGEQRQDGAQSDHVYGCYMHGIFDMSETVKTFLAAVLRKKG
                     IDPSKITVRDRNAYKEEQYDRLASIVRENLNMEAVYKIIEKGNEASCSLLSE"
BASE COUNT          630 a          763 c          448 g          924 t
ORIGIN      
        1 ttccacaatc ttctctgtca ttgcatccat atccgcccgt tttgcgactt gaatcttcat
       61 tccgtatttc cttgcttcgg ccgctgtttg ttctccgata caaacggcag acacattttg
      121 aagttctacg ttttttattg ctttgacaaa cccttttacc gttgaggcgc ttgtaaaagt
      181 aactgcatcg atctcctctt tttggaacat cttttctatc ttgtgcctaa gtaaagagga
      241 gacttcatat tcggtccgat ataatgcaat atcgtttacc gggacgcctg tttccataag
      301 aggcggaagc agttctaaag atccttcctc tgcccgaaag atcgtaaccg cactatactc
      361 tgaaatattt tctgcaagtg tttttcccaa ctctcctgca ttataaatct tcggcacgat
      421 atctgcaaac agtccgtgtt cttttaatgc agccgccgtc gcagaaccga tggccgcgat
      481 ttttatctgt gcttttcctg cgagcagcct tctcatatcc catgcttctt tttctaattg
      541 ttcaaaaaat acatgtactc ctataggact tgtaaaaaca aaccattcct ctttttcgga
      601 atgttgaatt tcccctaatg cctttttcag cctctcattc ggatcgatcg ctactgtatg
      661 aatagacggc atctcgatca cctgtgcccc gagatttcgc aggcgttttg ccagagcgga
      721 actattctgc ctcggtcttg tcaccaaaaa ttgctttcct cccaaaatcc tgtcttttat
      781 ccagtgaaac tcttctgaca aagaacacac ttttcctact acgataatcg caggcgcttt
      841 gatcttcgcc tcatctgcct gctgctttaa tgtttttaca gtcgcacaca cctgcctttg
      901 tctcgcagtc gttccccgct ctatcacagc cgcaggcata tcttccggca ttccggcttt
      961 tatcagttcc gtcagaatcg tttccattgc cgacacaccc ataagaaaga tcaaagttgc
     1021 atttaatttt acaagagcct cgtaatcaat gtcgggtttt acatctcttc tcgtatgtcc
     1081 tgtaatcaca tgaaaggatg aggtataatc tcgatgagtc accggaatcc ccgcataagc
     1141 cggaactgct actgaagaag tgattcccgg aataatttcg aaagaaatct tttcatttct
     1201 aagtatctct atttcttctc cgcctcttcc gaacacgaat ggatctccgc ctttgagacg
     1261 caatactctt ttcccttttt ttgcttctct tacgagaatc tgattgatct cttcttgagg
     1321 aaccggatgg tttcccgcat gttttccgac ataaattgtt tctgtttccc gcggaatacg
     1381 gcttagtatc tctgcgctta aaagagcgtc atatacgatg acatctgcct cttcgatcaa
     1441 ttccgcagtc cgcaaagtca gtaatcccgc atctcccgga cctgcccctg cgatccatac
     1501 ctttccatat aaactctgtt ccatctttct cacctttccc caaactgctt tttcattgtc
     1561 aaggcaagtc tttctccgag acttgaagcg tcttcaatat aagaagctgc ctttttcaca
     1621 aaatactgtc cgcttttttc attgtaatac agtcctgtca gttccaattc atttcccttt
     1681 acttttgcat aagccgcact cggagatgtg catcccccgt caagcacacg gataaaacta
     1741 cgttctgcca gagcctgtac tctgctcttt ttatcatcca catattgacg ccacactgta
     1801 tctttcaaaa attcttttct cccctgaact gccaaaattc cctgtcccgc agcagggatc
     1861 acctcatcta ccgaaaagta ccttccgatc accgcttcca tattcagccg cttcacccca
     1921 gcggctgcaa gcaccgccgc atcgtattct tctttgttta atttactaag tcttgtctgt
     1981 atgtttccgc ggattccttg aaacactgca ttcggataca atttctgaat ctgtatcatg
     2041 cgccttttac tggaagttcc tataacagcg cctgtttttg tgagtataga cccttcctct
     2101 gttccctgtt cttttttttc acttttataa ataagcacat cccttggatc ttcccgtctg
     2161 ctgtatgcaa gaatcggaaa ttcagaagat tcttccatcg gcatatcttt tagactgtga
     2221 acggcaagat cgatccttcc gtctgccaga gcctgatcta attcttttac aaaaagtcct
     2281 tttcctccga cagattccaa actcctgtca aggatcttat cccctgtcgt tttcatcgtg
     2341 atgatctcaa ctaaaatcgt ttggtcacat cgcctaatct gatcttttat aatttccgcc
     2401 tgctttaccg caagcctgct ttctctgctc cctattctga taacagactg catgacgctt
     2461 cgtttccttt ctctataatc ttatacacag cctccatgtt caggttctct cttactattg
     2521 acgcaagccg gtcatactgt tcttctttat atgcgtttct gtcacgcact gtaattttcg
     2581 acggatctat tccttttttt cttaaaactg ccgccaaaaa tgttttgacc gtttcagaca
     2641 tatcgaaaat cccatgcata taacagccat atacatgatc gctctgcgct ccgtcctgcc
     2701 gctgttctcc ttcctcttcc atatagacaa ccggaacttc tcgctttcta tatcagcctg
     2761 cattt
//
LOCUS       DBMDXF010000236         2740 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8087_length_2740_cov_2.174308, whole genome shotgun sequence.
ACCESSION   DBMDXF010000236 DBMDXF010000000
VERSION     DBMDXF010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8087_length_2740_cov_2.174308"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="ACZPD4_08085"
     CDS             <1..918
                     /locus_tag="ACZPD4_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1379048.1"
                     /translation="IPGLRVDEKSFVLKKKAIGLIRTKIIPTYMSWFANQCDLVLAPS
                     AGMQKILWEYGMRSRSAVFPTGLEKSFFAMDEQKAKEIRKTYKGDKKHLFVTVSRLEK
                     EKNYEFILRGIAKIKEEAGDDFHVMILGDGSQKGELKTKAALLGIGNLVTFVGNVKNE
                     DVKHYVHAADLFLFASKSETQGIVLAEAMAAGTPVVAVHAVGSDDIVKDGVNGFLTEE
                     EESRWAEKVIEAAQPENRFKMKKAALKEAQNYRSDTLALYEEMLYNQCICGKAMDRQK
                     DGKGEYSYENGEAHGEGAAMAVHKISHIA"
     gene            851..1483
                     /locus_tag="ACZPD4_08090"
     CDS             851..1483
                     /locus_tag="ACZPD4_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379049.1"
                     /translation="MKTEKLTEKALRWLFTKYLTLLEKTVKINWHEENEYGESQIFGF
                     WHEDSFFMNLVLERLADKTTPVDVIVTADTRGNYIEHMIKRCGGNALRVPDGYKAFGA
                     LKNIVRDSYEKAHSIAVALDGPLGPRHEPKKLAFYLSEHAEEAFVGISLSYSSCIRLT
                     RRWDKYVIPLPFTTVDVAVKDYGIVQKKAIPNLPVNADAASEEIYVAQTI"
     gene            1558..2256
                     /locus_tag="ACZPD4_08095"
     CDS             1558..2256
                     /locus_tag="ACZPD4_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1379050.1"
                     /translation="MEINHVLIVEDDKEIREGVQIYLQSQGYVVFQAADGIEGLEILE
                     KEEIHLAIIDIMMPRMDGIRMTMKLREKYDFPVIMLSAKSEEVDKITGLNIGADDYVT
                     KPFTPMELMARVNSQLRRYRKFLEKLAPQENTHVIGGLEINEDMVEVSMDGNPVKLTP
                     IEYKILLLLAKNPGRVFSAEEIYERVWQEKAINTDTIMVHVRNIREKIEIDPKNPKYL
                     KVVWGVGYKIEKQP"
     gene            2228..>2740
                     /locus_tag="ACZPD4_08100"
     CDS             2228..>2740
                     /locus_tag="ACZPD4_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1379051.1"
                     /translation="MDTKSKNSRKFGILILILLLAASSAVMMLQYGTIRRQIESVQEE
                     GSRSGETAADMANTLAEGNYLLYNRYVRDTDPAEVLSLYGQQRFDLAEKYLDYELIDS
                     KDEVIASDLPEEEQEKLHEDGKEYAFRVKYIFSENGDLEDIQIGGTAFGAEEAYNMES
                     IYINEVESVES"
BASE COUNT          882 a          457 c          750 g          651 t
ORIGIN      
        1 ataccgggac tgcgagttga tgagaagagc tttgtattaa aaaagaaggc gatcggactg
       61 atcaggacaa agatcatacc aacttatatg agctggtttg caaatcagtg cgatctcgta
      121 cttgctccgt cggcaggaat gcagaagata ttgtgggaat atggtatgag aagcagatcc
      181 gccgtatttc cgaccggact tgaaaagtcg ttttttgcga tggatgagca gaaggcaaaa
      241 gagatcagga agacgtataa gggagataaa aaacatttat ttgtcactgt atccagactt
      301 gagaaagaga aaaattatga atttatcctt cggggaatcg cgaagatcaa agaagaagcc
      361 ggggatgact ttcatgtgat gatacttgga gacggaagtc agaagggaga attaaaaacg
      421 aaagccgctc ttttagggat cggaaatctt gtgacttttg taggaaacgt gaagaatgaa
      481 gatgtaaaac attatgtaca tgcggcagat ctgtttttat ttgcatcaaa atcagagaca
      541 cagggaatcg ttctggcaga agcgatggca gcaggaactc cggtagttgc ggttcacgcg
      601 gtcggatcgg acgatattgt caaagacgga gtaaacggat ttttgacaga agaagaggaa
      661 agcaggtggg cggagaaagt catcgaagca gcacagccgg agaaccggtt taaaatgaaa
      721 aaagcggctt tgaaagaagc acaaaattat cgttccgaca cacttgcctt atatgaagag
      781 atgctctaca atcaatgtat ctgcggaaaa gcaatggatc ggcaaaaaga cggaaaagga
      841 gaatattctt atgaaaacgg agaagctcac ggagaaggcg ctgcgatggc tgttcacaaa
      901 atatctcaca ttgcttgaaa agacagtaaa aataaactgg catgaagaaa atgaatacgg
      961 tgaaagtcag atattcggat tttggcatga ggacagcttt tttatgaatc tcgtgcttga
     1021 gcgtctggcg gataagacaa cacctgtaga tgtgattgtt acagccgata cacggggaaa
     1081 ttatattgaa catatgataa agcgatgcgg cggaaacgcg cttcgcgttc ctgacgggta
     1141 taaggcgttc ggggcgttaa aaaacatcgt ccgcgattct tatgagaagg cacattcgat
     1201 cgccgttgcg ttagacggac cgctcggtcc gcggcacgag ccgaaaaaac tggcatttta
     1261 cttgtcggaa catgcagaag aagcatttgt aggcatcagc ctttcttatt cttcatgtat
     1321 caggctgact cgcagatggg ataaatatgt aattccgctt ccgtttacaa cagtggacgt
     1381 tgcggtaaaa gattatggga tcgttcagaa gaaggcgatt ccgaatcttc cggtcaatgc
     1441 agatgcagcg tcggaagaaa tatatgttgc gcagacaata taaacatttc tcttttgcaa
     1501 aaagaagaga taggataaaa aatacgggga atgacggaaa aggagagggc ataagcaatg
     1561 gaaatcaatc atgttttgat cgtagaggat gataaggaaa tcagggaagg cgtacagatc
     1621 tatctgcaaa gtcaggggta tgtcgtattt caggcagcag atggaattga aggtttagaa
     1681 atacttgaaa aagaggagat ccatctggcg atcatagata ttatgatgcc gcgtatggac
     1741 gggatacgca tgacgatgaa actaagggag aaatacgatt ttccggttat catgttatct
     1801 gccaaatccg aggaggtcga taagatcaca ggtcttaata tcggggcgga tgattatgtg
     1861 acgaaaccgt ttactccgat ggaactgatg gcgcgagtga attcccagtt acgaagatac
     1921 cggaagtttc ttgaaaaatt agcacctcag gaaaatacgc atgtgatcgg cgggcttgag
     1981 atcaatgaag atatggtaga agtgtctatg gacggaaatc ctgtgaagct gacgccgatc
     2041 gagtacaaga tccttttatt gcttgccaag aatccgggaa gagttttttc ggcagaagag
     2101 atttatgagc gggtttggca ggaaaaagcg atcaatacag atacgattat ggtgcatgta
     2161 agaaatattc gggagaagat cgagatcgac ccgaaaaatc caaaatattt gaaggtggtg
     2221 tggggcgttg gatacaaaat cgaaaaacag ccgtaaattt ggaattctta ttctgatctt
     2281 gcttttggcg gcaagttccg cagttatgat gctgcaatac ggaacgatca gacggcagat
     2341 agaatcggtg caggaggagg gctcccgatc gggagaaacg gcggcggata tggcgaatac
     2401 gctggcggaa ggcaattatc ttctttataa tcggtatgta cgggacactg atccggcgga
     2461 agtactttct ctttacggac agcaaaggtt tgatctggca gaaaaatatt tagattatga
     2521 gctgatcgac agtaaagatg aggtgatagc ttctgatctg ccggaagaag aacaggagaa
     2581 gctgcatgaa gacggaaaag agtatgcctt ccgtgtgaaa tatatttttt ccgaaaacgg
     2641 agatttggag gatatccaga tcggcggaac ggcattcgga gcagaagagg catacaatat
     2701 ggaatcgatc tatattaacg aggttgaaag cgtcgagtct
//
LOCUS       DBMDXF010000237         2738 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8094_length_2738_cov_1.759954, whole genome shotgun sequence.
ACCESSION   DBMDXF010000237 DBMDXF010000000
VERSION     DBMDXF010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8094_length_2738_cov_1.759954"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="ACZPD4_08105"
     CDS             complement(<1..410)
                     /locus_tag="ACZPD4_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1379052.1"
                     /translation="MSETFTLYKLIILYMLNKVDFPLTTSQISEFVLDKGYTTYFKLQ
                     EAISELTQSELIRTETTHNRTLYHLTESGAETIHYFSNKISSAIRKDIDDFLTEKQYD
                     LKEEVSVKADYYLNTNQEYETRCQIVENGFSLID"
     gene            complement(403..1347)
                     /gene="mnmL"
                     /locus_tag="ACZPD4_08110"
                     /gene_synonym="ytqA"
     CDS             complement(403..1347)
                     /gene="mnmL"
                     /locus_tag="ACZPD4_08110"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845764.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR1379053.1"
                     /translation="MAPVFSQFQPDGERYRSLNLYLKKLFGEKVYKVTLNGGMTCPNR
                     DGHIGTGGCIFCSAMGSGDFAGNASLSITEQLREGKDAIRKKRPVRKFIAYFQAFTNT
                     YAPVDHLEKIFTEAINDPDVCALSIATRPDCLPPAVLELLGRLNRIKPVWVELGLQTV
                     NSETARFIRRGYPTDVFDHAAHRLRMLGITVIVHVILFLPYETKEMMFHTIDHLNKLD
                     IQGIKLQLLHILKGTDLAAVYEKTHFHVPDMEEYIELLGSCIAKLRPDIIVHRLTGDG
                     PKELLIAPLWTSAKRTVLNRFHQYLKQQDIWQGKDYHV"
     gene            complement(1347..2495)
                     /locus_tag="ACZPD4_08115"
     CDS             complement(1347..2495)
                     /locus_tag="ACZPD4_08115"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1379054.1"
                     /translation="MSNSEKALQMHEQWNGKLETTAKAHVNSREDLTIAYTPGVAEPC
                     KVIAKDPEAAYKYTIKSNTVAVVSDGSAVLGLGNIGALAAMPVMEGKAVLFKEFGGVN
                     AVPICLDTQDTEEIIRTVVNIAPAFGGINLEDISAPRCFEIETRLKELLDIPVFHDDQ
                     HGTAIVVLAGIINALKVTKKEKETCRVVVNGAGSAGVAITKLLLNYGFKDITMCDING
                     IISKDSENLNWMQKEMAKVTNLKNASGTLADALKGADIFVGVSAPGIVTKDMVASMNK
                     DAILFAMANPVPEIMPDLAKAAGAKVVGTGRSDFPNQVNNVVAFPGIFKGALEGRASQ
                     ITEEMKLAAAKAIAGLVPDEELSEDNILPQAFDPCVAETVSRAIKELI"
BASE COUNT          697 a          585 c          613 g          843 t
ORIGIN      
        1 aagtcgatca ggctgaaacc gttttctacg atctgacatc ttgtttcgta ttcctgattt
       61 gtgtttagat agtagtcggc ttttacagat acttcttctt ttaagtcata ttgtttttcg
      121 gtcagaaaat cgtcgatatc cttgcgtatg gcggatgaaa ttttattgct gaaataatgg
      181 atcgtttccg caccgctctc tgtgagatga tagagagtcc gattgtgggt tgtctcggtg
      241 cggatcagct cggactgcgt cagttcggag attgcctcct gcaatttaaa ataagttgtg
      301 taccctttat ccagtacgaa ttccgatatc tgggaagtgg tgagcggaaa atcaactttg
      361 ttcagcatat agagtatgat tagtttatac agtgtaaatg tttcagacat gataatcctt
      421 tccctgccaa atgtcttgtt gctttaaata ttgatgaaac cgatttaaga cggttcgttt
      481 tgcgcttgtc cataacggcg cgataagaag ttctttaggt ccgtctcctg tcagtctgtg
      541 aacaatgata tcagggcgca gttttgcgat acatgacccg agcagttcaa tatattcttc
      601 catatcaggt acgtgaaaat gtgttttttc gtaaacggct gcgagatctg ttcctttcaa
      661 tatatgaaga agctgcagtt taatcccctg aatatcaagt ttgttcaaat gatcgatcgt
      721 atggaacatc atttctttcg tttcgtacgg aagaaagagg atgacgtgaa cgatgacagt
      781 gattccaagc atacgaagcc ggtgcgccgc atgatcaaat acatccgtcg gatatccgcg
      841 ccggatgaaa cgtgccgttt cggaattgac cgtctgaagt cccaactcta cccagacagg
      901 tttgatccgg tttaatctgc caagcagctc aagtacggca ggaggcagac aatcgggtct
      961 ggtggcgatt gagagagcgc agacatcggg atcgtttatt gcttcggtaa atattttttc
     1021 aagatgatct acaggagcgt aagtgtttgt aaacgcttga aaataagcga taaatttgcg
     1081 tacggggcgc ttttttctga ttgcgtcttt gccttccctt agttgttcgg taatagaaag
     1141 cgaagcgttt cccgcaaagt caccggatcc catcgcactg cagaaaatac atccgccggt
     1201 tccgatatgt ccgtctctgt tcggacatgt catccctccg tttaaagtga ccttatatac
     1261 tttttctccg aacagtttct taagatagag attcagggaa cgataacgtt ccccatctgg
     1321 ctgaaactgt gaaaaaacag gcgccattaa atcaattcct ttattgcgcg gctgactgtt
     1381 tccgcaacac atgggtcaaa tgcctgaggt aaaatattat cttcggaaag ttcttcatcc
     1441 gggacaagtc cggcgatcgc ttttgcagcg gcgagtttca tttcttctgt gatctgagaa
     1501 gcacggcctt ctaaagcgcc tttaaagata cccggaaaag ctactacgtt atttacctga
     1561 ttcggaaaat ccgaacgtcc cgttccgacg actttcgcac ctgcggcttt ggcaagatca
     1621 ggcatgatct cgggaacagg attcgccata gcgaacaaaa tggcatcttt attcatagaa
     1681 gcaaccatgt cttttgtgac gatgccggga gcggaaacac cgacaaaaat atctgcgcct
     1741 tttaaagcgt cggccaaagt tcccgaagca ttttttaaat tagtcacttt tgccatttcc
     1801 ttttgcatcc agtttaagtt ctctgaatct tttgagatga ttccgttaat atcgcacatt
     1861 gtaatgtctt tgaaaccgta atttaaaaga agtttcgtaa tcgcaactcc ggcgctgccg
     1921 gctccgttta cgacaacgcg gcatgtctcc ttctcttttt tggttacttt cagggcgttg
     1981 atgattccgg caagtacgac gatagcggtt ccgtgctgat cgtcatggaa aacaggaata
     2041 tcaagcagtt cttttaaacg tgtttcgatt tcaaaacagc ggggagccga gatgtcttcc
     2101 agattgattc ctccgaatgc cggagcaata tttacgacag tacggatgat ctcctctgta
     2161 tcttgggtat caagacagat cggtactgca ttgacaccgc cgaattcttt gaaaagtacg
     2221 gcctttcctt ccataacagg cattgcggca agagcgccga tgtttccgag gccaagtacg
     2281 gcgcttccgt cggaaacgac tgctacagtg ttggatttga ttgtatattt gtatgcggct
     2341 tccggatctt ttgcgataac tttacacggt tccgctactc ccggagtgta agcgatcgta
     2401 agatcctcgc gggaattgac gtgggctttt gctgttgttt ccagtttccc gttccattgt
     2461 tcatgcatct gaagtgcttt ttcgctattt gacattttga ttacctcgct tgtaattatc
     2521 gttttgcaca tatgctatta tcatagcact tctccccccg tcttgacaag ggcatgaacg
     2581 aaatgtataa taagtacaca tgtttagtga attcatcaat tttatgtcaa ttcagacagt
     2641 gattcccgga gcatttagaa aaaattgaag aaaccgattt gtttatattt cttatatttc
     2701 atgaaagggt tggattatga caagagaaaa atatgaat
//
LOCUS       DBMDXF010000238         2737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8097_length_2737_cov_1.852807, whole genome shotgun sequence.
ACCESSION   DBMDXF010000238 DBMDXF010000000
VERSION     DBMDXF010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_2737_cov_1.852807"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /gene="uvrA"
                     /locus_tag="ACZPD4_08120"
     CDS             complement(<1..1907)
                     /gene="uvrA"
                     /locus_tag="ACZPD4_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1379055.1"
                     /translation="MSTKMDVRKYIKIRGANENNLKNIDVDIPRNELVVLTGLSGSGK
                     SSLAFDTIYAEGQRRYMESLSSYARQFLGQMEKPDVESIEGLSPAISIDQKSTNHNPR
                     STVGTVTEIYDYFRLLYARVGIPHCPKCGKAISKQTVDQMVDRIMSMPEGTKIQLLAP
                     VVRGRKGTHAKLLEKAKKSGYVRVRVDGNMYELTEEIILDKNLKHNIEIIVDRLIVKQ
                     GIEKRLTDSVESVLHLSEGLLSVDVIGGEMLNFSQSFSCPDCGISIEEIEPRSFSFNN
                     PFGACPTCYGLGYKMEFSEDLMIPDPSLSINEGAIAVLGWQSCTDKSSFTHAILQALC
                     REYQFDLDTPFEKYPKKIHDIILHGTNGKEVKVFYKGQRGEGVYDVAFEGLIKNVERR
                     YKETGSETMKAEYETFMNITPCSACHGQRLKPGALAVTVGEKNIAEVTSMSIGKLQNF
                     LEELVLGETQMLIGGQILKEIRARIKFLMDVGLDYLTLARATGTLSGGEAQRIRLATQ
                     IGSGLVGVAYILDEPSIGLHQRDNDKLLATLKHLRDLGNTLIVVEHDEDTMMEADYIV
                     DIGPGAGEHGGEVVAVGNAKEIMKNENSITGAYLSGRIKIPVPQERRQPKGWLKVVGA
                     RENNLKNIDVS"
     gene            complement(1951..>2737)
                     /locus_tag="ACZPD4_08125"
     CDS             complement(1951..>2737)
                     /locus_tag="ACZPD4_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR1379056.1"
                     /translation="RQRIVVEQVIRPTGLLDPEVVVRPVEGQIDDLIGEINKEISQKN
                     KVLVTTLTKRMAEDLTDYMREVGIRVKYLHSDVDTLERTEIIRDMRLDVFDVLVGINL
                     LREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAARNAQGHVIMYADKITESMQL
                     AIDETKRRREIQMKYNEEHGITPKTIQKSVRDLISISKKVAAEELKLEKDPESMSVKE
                     LKKLIADLTKQMKKAAAELNFESAAELRDKLTELKKMLNEIEG"
BASE COUNT          686 a          736 c          427 g          888 t
ORIGIN      
        1 aatgacacat cgatattttt caagttgttt tcccgggcgc cgaccacctt caaccatccc
       61 ttaggctgtc tgcgctcttg cggaaccgga attttgatcc ttcccgataa gtatgcgcct
      121 gtaatcgaat tttcattttt catgatctct ttcgcatttc cgacggcaac aacctcgcct
      181 ccgtgttctc ccgctcccgg tcctatatct acaatataat ccgcctccat cattgtatct
      241 tcgtcatgct ctacgactat cagcgtattt cccagatcac gaagatgctt taatgttgca
      301 agaagcttgt cattatcccg ctgatgaagt cctatacttg gctcatccaa aatatatgct
      361 actcccacaa gacccgatcc gatctgcgtt gccagacgta ttctctgcgc ctcaccgcct
      421 gaaagtgttc cggtagcgcg ggccaacgtc aagtaatcaa gtcccacatc cataagaaat
      481 ttgatccgag ctctgatttc tttcaaaatc tgccctccga tgagcatctg tgtttctcct
      541 aatacaagct cttcaagaaa attttgcaat tttccgatag acatcgacgt tacttctgca
      601 atattttttt ctccgacggt taccgccaaa gctcccggtt tcagacgttg cccatggcat
      661 gccgaacacg gagttatgtt cataaatgtc tcatattcgg ctttcatcgt ctccgaaccg
      721 gtttctttat atctgcgctc tacgttttta atcaaccctt caaacgcaac atcataaacg
      781 ccttcgcctc tctggccttt ataaaatact ttcacctctt tcccgtttgt gccatgaaga
      841 ataatatcat gaatcttctt cggatatttt tcaaacggtg tgtccagatc aaattgatac
      901 tctctgcaca gcgcctgaag tatcgcatga gtaaaacttg atttgtctgt acaagactgc
      961 caccctaata ccgctattgc accttcatta atcgacaatg acggatccgg aatcatcaaa
     1021 tcctctgaaa actccatctt atatcctaat ccataacatg tcggacatgc cccaaaagga
     1081 ttattaaatg aaaaacttct tggttcgatc tcttcgatac tgatcccaca atccggacag
     1141 gaaaagctct gactgaaatt caacatctcc ccgccgatca catctacact gagcaaacct
     1201 tccgataaat gcagaacact ttccacagaa tctgttaacc tcttctcaat cccctgtttt
     1261 acgatcaatc ggtcaacaat aatctcaata ttatgtttca agttcttgtc aagaataatc
     1321 tcttccgtca attcgtacat atttccgtct acacgtacac gcacatatcc gcttttcttt
     1381 gctttttcca ataattttgc atgtgttcct ttccggcctc ttacgaccgg agcaagaagc
     1441 tggattttcg ttccttccgg cattgacata attcgatcaa ccatctgatc gactgtttgc
     1501 tttgaaattg cttttccaca tttaggacaa tgaggaattc cgactcttgc atataaaagt
     1561 ctgaaataat cataaatttc agtcactgtt ccgaccgtag atctcggatt gtgatttgtc
     1621 gacttttgat caatggaaat tgcaggcgac agtccctcta tgctttccac atccggtttc
     1681 tccatttgcc ccaaaaactg tcttgcatag gaagataatg attccatata tcttctctgc
     1741 ccttctgcat aaatcgtatc aaacgcgagg gaagatttgc ccgaaccgct caatcctgta
     1801 agtacaacaa gttcgttccg gggaatgtcc acatctatat ttttaagatt attctcattc
     1861 gctcccctta ttttgatata tttccgaaca tccattttcg tactcataat ccgtcttttt
     1921 cctccctgta gtcatatttt attttgtatt ttatccttct atttcattca acattttttt
     1981 caattctgtc aacttatccc gcaattctgc cgcgctttca aaattcagtt ctgccgccgc
     2041 tttcttcatc tgttttgtaa gatcggcaat cagcttttta agttctttta cgctcataga
     2101 ttccggatct ttttccagtt tcagctcctc tgccgcaact tttttagaaa tactgatcag
     2161 gtcgcgtact gatttttgaa tcgtctttgg tgtgatacca tgctcttcat tatatttcat
     2221 ctgaatctca cgacgccgtt ttgtttcatc gatcgccagc tgcatagatt ccgttatctt
     2281 atccgcatac ataataacat gcccttgtgc attacgcgcc gcacgtccta ttgtctgtat
     2341 caatgacgtt tccgaacgca aaaatccttc cttatccgca tccaatatcg ctacaagcgt
     2401 aatttccgga atatccagac cttctcgcaa aagattgatt cccacaagca catcaaatac
     2461 atctaaacgc atatcacgaa taatctctgt acgttccagt gtatccacat ccgaatgcaa
     2521 atatttcaca cgaattccca cctcacgcat ataatccgtc aaatcttctg ccatccgctt
     2581 tgtcagagtt gttacgagaa ctttattctt ttgggaaatt tctttgttta tttccccgat
     2641 cagatcgtct atctgacctt ctacaggacg cacgacaact tccggatcga gcagtccggt
     2701 cggacgaatc acctgttcca caacgatccg ctgccgc
//
LOCUS       DBMDXF010000239         2722 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8162_length_2722_cov_1.793590, whole genome shotgun sequence.
ACCESSION   DBMDXF010000239 DBMDXF010000000
VERSION     DBMDXF010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8162_length_2722_cov_1.793590"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="ACZPD4_08130"
     CDS             <1..1319
                     /locus_tag="ACZPD4_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1379057.1"
                     /translation="HEQRKRKCEIERVTEGCTGIRRSTGQHPGGIVVLPHGHDINEFT
                     PVQHPANDMECGITTTHFDYHSIDHNLLKLDILGHDDPTMIRTLEDYITSDAMENEYN
                     ADHPFIATEIPLDDKDVIELFHGTEVLGIKPEDIDGCKIGSLGIPEFGTDFVIQMVQD
                     TKPQTLSDLIRISGLSHGTNVWLGNAQELVKSGKATISTAICTRDDIMIYLINKGVES
                     ALAFTIMESVRKGKGLKPEWEEAMKAQDVPDWYIWSCKKISYMFPKAHAAAYVMMAYR
                     IAYCKINYPLAYYAAYFGIRADAFSYALMCQGKETLNYHIKQYKSKGDQLSKKEQDVL
                     KDMKIVQEMYARGFEFVPIDIYRAKAKMFQIVDGKLMPSLASIEGMGDKAAEAVEEAA
                     KDGAYLSLDDFRQRTKVSKTVIDLMAELGLFGDLPQSNQLSLFDFT"
     gene            1386..1838
                     /locus_tag="ACZPD4_08135"
     CDS             1386..1838
                     /locus_tag="ACZPD4_08135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1379058.1"
                     /translation="MIRKFQRQDLEAAVKIWLAANKEAHDFIDPCYWEGYKDAVKEMF
                     LQAEIYVFETEDMMEEIFDETGGILGFIGLDKDYVEGIFVRGDVRSQSIGKQLLDFVK
                     QKRKRLELNVYVKNKRAVQFYEREGFCIQREGKDAETGEKEYCMLWIK"
     gene            1927..2619
                     /locus_tag="ACZPD4_08140"
     CDS             1927..2619
                     /locus_tag="ACZPD4_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1379059.1"
                     /translation="MEHRNSVLIVEDDVNINGLLKEALEKEGYICTQAFSGTEARMIL
                     QGERYSVILLDLMLPGIPGEEVLRDIRKKGNTPVIILTAKDTIDDKVEFLRGGADDYV
                     TKPFDIKEVTARVEVQIRRAETTGRKEEEHRFTYQELELNKGDFTVKVGNTELPHITK
                     QEFSILELLLKHPKKVFSKEEMFEYAWEEQYMGETKTLDVHISNIRKKIKAVTDKEYI
                     ETVWGIGYRLHP"
BASE COUNT          942 a          385 c          710 g          685 t
ORIGIN      
        1 aacatgagca aagaaaacga aaatgtgaga tagaaagagt gaccgaaggg tgtacgggaa
       61 tccgaagaag cacaggtcag catccgggag gtatcgtcgt tcttcctcac gggcatgata
      121 tcaatgaatt tactccggtt caacatccgg caaatgatat ggaatgtgga attacaacga
      181 cacattttga ttatcattcc atcgatcata acttgttgaa gttagatatt ctgggacatg
      241 acgatccgac gatgatccgt acactggagg actatattac atcggatgct atggaaaatg
      301 aatacaatgc agatcatccg tttattgcga ccgaaattcc tcttgatgat aaggatgtga
      361 tcgaactgtt tcatggaaca gaagtgttgg ggattaagcc ggaagatatt gacggatgta
      421 agatcggaag tctcggaata ccggaattcg gaacggactt tgttattcaa atggttcagg
      481 atacgaaacc gcagacactt tcggatctga tccgtatttc cggattatcg cacggaacga
      541 atgtgtggct tggaaatgca caggagcttg tgaagtcggg aaaggcgacg atttcgacgg
      601 cgatttgtac gagagacgat attatgatct atcttatcaa caaaggagta gaaagtgctc
      661 ttgcgtttac gattatggaa agtgtgcgaa aaggaaaagg actgaaaccg gaatgggaag
      721 aggcgatgaa agcacaggat gtgccggatt ggtatatttg gtcatgtaaa aaaattagct
      781 acatgttccc gaaagcacat gccgccgctt atgttatgat ggcatatcgg atcgcatact
      841 gtaagatcaa ctatcctctt gcatattatg cggcgtattt cggcatcagg gcggatgcgt
      901 tttcgtatgc gcttatgtgt cagggaaaag aaacgttgaa ttatcatata aaacagtata
      961 aaagtaaagg ggatcagcta agcaagaaag agcaggacgt gctaaaagac atgaagatcg
     1021 ttcaggaaat gtatgcccgt ggatttgagt ttgttccgat tgatatttat agggcgaagg
     1081 caaagatgtt tcagatcgta gacggaaagc tgatgccgtc gcttgcaagc attgaaggaa
     1141 tgggggataa agctgccgag gcagtggaag aggcggcgaa agacggggcg tatctgtcgc
     1201 ttgacgactt caggcaaaga acaaaagtca gcaagactgt gattgatctg atggcggagc
     1261 ttggattgtt tggagatctt ccgcagagta atcagttatc gctgtttgat tttacatgat
     1321 ggcagaaact ttttaaacgc cgcggaatgc ggagaataaa aagataagat gcaggagaga
     1381 aagacgtgat cagaaaattt caaagacagg atctggaagc ggcagtgaaa atctggcttg
     1441 ccgcgaataa agaagcgcat gattttattg atccgtgtta ttgggaaggt tacaaagatg
     1501 cggttaaaga gatgtttttg caggcggaaa tctatgtttt cgagacagaa gacatgatgg
     1561 aagagatatt tgatgaaaca ggcggaattc tcggctttat cggattagat aaagattatg
     1621 tggaggggat ttttgtcaga ggagacgttc gctcccaaag tatcggaaag cagcttcttg
     1681 attttgtaaa acaaaagaga aaaagattag aattaaacgt atatgtaaaa aataaaaggg
     1741 cagtacaatt ttatgaaagg gaaggatttt gtattcaaag agaaggaaaa gatgcggaaa
     1801 ccggagaaaa agaatattgt atgctatgga taaaataaat gacttttata tgaatccgga
     1861 gaaaatataa acaaggcgta tgaaaacaga ggaataaaaa catctgttac ataaatgagg
     1921 atggagatgg aacatagaaa ttcagtatta atcgtagaag atgatgtaaa tattaacgga
     1981 cttttaaaag aagcattaga aaaagaaggg tatatatgta cgcaggcgtt ctcaggtacg
     2041 gaagcaagaa tgattttaca gggggaaaga tattcggtta tacttcttga tttaatgctt
     2101 ccgggaattc cgggggaaga agtacttaga gatattcgaa agaaaggaaa taccccggtg
     2161 atcattctaa cagcaaaaga tacgattgat gataaagtgg aatttttgcg cggcggagcg
     2221 gatgattatg tgacaaagcc gtttgatatt aaagaagtaa ctgccagagt ggaagtgcag
     2281 atcagaaggg cagaaacaac agggagaaaa gaggaggaac accgatttac ttatcaggag
     2341 ctggaactta ataagggtga ttttactgta aaagtgggaa atacagaatt gcctcatatt
     2401 acaaaacagg aattttcaat attggagctg ttgttaaaac atccgaaaaa agtattcagc
     2461 aaagaagaaa tgtttgaata tgcgtgggaa gagcaatata tgggagagac aaagacgcta
     2521 gacgtacata taagtaatat ccgcaagaag atcaaggctg tgacagataa agaatatatt
     2581 gaaactgtat gggggatcgg gtacaggctt catccgtaat ctttactctt tttttacttt
     2641 ttatttgcga ttgattagca atttgtttat agaatgagag cagataaaga aaagaaaagg
     2701 agaaaaaaga tgagtgaata tt
//
LOCUS       DBMDXF010000240         2717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8183_length_2717_cov_1.544343, whole genome shotgun sequence.
ACCESSION   DBMDXF010000240 DBMDXF010000000
VERSION     DBMDXF010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8183_length_2717_cov_1.544343"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="ACZPD4_08145"
     CDS             <1..841
                     /locus_tag="ACZPD4_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1379060.1"
                     /translation="KAFAVGDIGGNSILVFSEEGLKGEIETSLPIENMAISDQGIVTV
                     LLKNETAPKIISYDAMGNVLVEQQVTVPVMGYPVAMDMSDDGKMLAVTYFHTDDAVLK
                     SKVIYYNFGESGKDKPDKIVASDEYSDTIMADIFFMGSDRSVVVGDNSIVIYKGKAEP
                     KKEKEISIGQEIQSVFHSDQYIGLILLNSEKSGYELRLYDRFGERILNKEISGRYENA
                     KIEGSEVIMYEGSKCCIVTANGIVKFEGDIKTEALEIFKASGLNRYYVMSADELRVIY
                     LTK"
     gene            850..1668
                     /locus_tag="ACZPD4_08150"
     CDS             850..1668
                     /locus_tag="ACZPD4_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1379061.1"
                     /translation="MDNWLLIIVGTIFLICIAFGYVRGFLKLGLSLLSTILTLVLVLF
                     LSPHVTRALKEYTPVDDFLESKVTEKFMPEITSEQLQSIDLTGTPLENLTAEDISKLN
                     EMDWDVLGITADDILSVIGDIPKDVQINLIEEAPLPRFLKDQLIENNNSTIYGELGVK
                     SFPRYVAAYASHLVLNLLSFLVTFLLAIILVKALMFAVNIIGELPVLGLANHIAGGAL
                     GLLLALVIVWIGFLIMTLAYTTEAGSACFEMVEKSSILRFLYETNPLLIRLLKF"
     gene            1863..2543
                     /locus_tag="ACZPD4_08155"
     CDS             1863..2543
                     /locus_tag="ACZPD4_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1379062.1"
                     /translation="MEEKYKILIVDDEQSILDMLKLQLEFEGYSVYTAGNAAEALDKL
                     SYSPDIVLLDINMDGMNGLDLCVSIRDFISCPIIFLTARVSEQDKINGLMAGGDDYIT
                     KPFSVNELLARISAHLRREQRSHNKTKSKFSEDLIIDYSDRSLYIKGHKIELSNKEFE
                     IIQLLSSNAGQVFDREKIYENIWGIDGNGDSVVIKEHIRKIRLKFNEFTDKGYITTVW
                     GVGYKWEK"
     gene            2531..>2717
                     /locus_tag="ACZPD4_08160"
     CDS             2531..>2717
                     /locus_tag="ACZPD4_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1379063.1"
                     /translation="MGKIREVFQKMSLKRSLVLLAVFCLSLVSALSVITILTCSSIQK
                     KILDTRPIIITDYVIRDA"
BASE COUNT          842 a          401 c          646 g          828 t
ORIGIN      
        1 aaaagcgttt gcggtaggag atatcggagg aaacagcatt cttgtatttt ccgaagaagg
       61 tttgaaaggc gagattgaga caagtcttcc gattgaaaat atggcgattt cggatcaggg
      121 aattgtgaca gttctgctga aaaatgaaac tgcgccgaag atcatttctt atgatgcaat
      181 gggcaatgtt ttagtggagc agcaggtaac tgttcctgta atgggttatc ctgttgcgat
      241 ggatatgtct gacgacggaa agatgctggc ggtaacttat tttcatacag acgacgccgt
      301 gctgaagtca aaagtgatct attataattt cggagagagt ggaaaggaca agccggataa
      361 gatcgttgca tcggatgaat attcagacac gatcatggca gacattttct ttatgggaag
      421 cgaccgctct gttgtagtag gagataactc gatcgtcatc tataaaggga aagccgaacc
      481 gaaaaaagaa aaagagatat cgatcggaca ggagatacag agcgtatttc actcagacca
      541 atatataggg cttatattgc ttaacagtga aaaatcagga tacgagctgc ggctgtatga
      601 tcggtttggg gagaggatac ttaataaaga gatttcggga cgatacgaaa atgcaaagat
      661 agaaggcagc gaagttatta tgtatgaagg aagtaagtgc tgtatcgtga ccgcaaacgg
      721 aatcgtaaaa tttgaaggag acataaaaac agaggcgctg gagattttca aagcttcggg
      781 attgaacaga tactatgtga tgagcgcaga tgaattacgg gtaatttatc tgacgaaata
      841 ggagagatta tggataattg gcttttgatc attgtaggaa cgatttttct tatctgtatt
      901 gcttttgggt atgttagagg atttttaaaa ctcggacttt cccttttgtc aacaatattg
      961 actcttgttc ttgtgctgtt cctttcgccg catgttacac gagcgcttaa ggagtatacg
     1021 cctgtagatg atttcttaga aagtaaagtt actgagaaat ttatgccgga gataacgtct
     1081 gagcagcttc agtcgatcga tctgacagga acgccattgg aaaatcttac ggcggaagac
     1141 atctccaagt taaacgagat ggactgggat gtgctcggca ttacggcgga tgatatttta
     1201 agtgtgatcg gagatattcc gaaagatgtg cagatcaact tgattgagga agcccctctt
     1261 ccccggtttt tgaaagatca gcttatagaa aataacaaca gcacgattta cggagagctg
     1321 ggagtgaaaa gttttccgag atatgtggcg gcatatgcat cgcatcttgt attgaatctg
     1381 ttgtcgtttc ttgtgacatt tttgctggcg atcattcttg tgaaggcgct gatgtttgct
     1441 gtgaatatta ttggggaact tccggtgctt ggactggcaa atcatattgc cggaggagcg
     1501 ctcggtcttt tgcttgcgct tgtcattgtg tggatcggat ttctcatcat gacgcttgcc
     1561 tatacgacag aggcgggaag cgcatgcttt gaaatggttg aaaagagtag tattctgcgt
     1621 ttcttatatg aaacaaatcc gcttttgatc cgcctgttaa aattttaaat ctgtatagaa
     1681 gagggctgtt ggaaaaataa acaaaatgtt attttgtaac tgaaagttac tgtattttga
     1741 tgtacagtaa ctttttttat tttgtgataa aattaatttt aatagggaaa ttaaaaattg
     1801 gcgaaaaatt gacttttttc tgttataaga tttttaatct cattgataag gagtgaaagt
     1861 ttgtggaaga aaaatataag atcctaatcg tagatgacga acagtctatt ttagatatgt
     1921 tgaaattaca gcttgaattt gaagggtatt ctgtttatac tgccggcaat gcggcagagg
     1981 ctttggataa attatcttac tcgccggaca tagttttatt agacatcaat atggatggca
     2041 tgaacggttt ggatttatgt gtctctattc gtgattttat ctcttgtccg attatttttc
     2101 tcactgccag agtatctgag caggataaga tcaacgggtt aatggcaggc ggagatgatt
     2161 atattacgaa gccattttct gtaaatgaat tgctggcaag aatttcggcg catttacgac
     2221 gcgaacaaag aagtcataac aagacaaaaa gtaaattttc agaagatctt attattgatt
     2281 attctgatag aagtctttat atcaaagggc ataaaattga attgtcaaat aaagaatttg
     2341 aaattataca actcttgtct tctaatgcag gtcaggtttt tgacagagaa aaaatttatg
     2401 aaaatatttg gggcattgac ggcaatggag acagcgttgt tatcaaagag catatacgaa
     2461 aaatacgtct gaaatttaat gagtttacgg ataaaggtta tattactaca gtttggggag
     2521 tcggatataa atgggaaaaa taagagaagt atttcaaaag atgtcgctaa aacgatcatt
     2581 agtattgctg gcggtatttt gtcttagtct ggtcagcgct ctttcagtaa ttacaatatt
     2641 gacatgttca tctatccaga aaaaaatact tgatacaaga cctattatca tcacagacta
     2701 tgtaattaga gacgcag
//
LOCUS       DBMDXF010000241         2717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8184_length_2717_cov_1.491590, whole genome shotgun sequence.
ACCESSION   DBMDXF010000241 DBMDXF010000000
VERSION     DBMDXF010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8184_length_2717_cov_1.491590"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /gene="rpsA"
                     /locus_tag="ACZPD4_08165"
     CDS             complement(<1..997)
                     /gene="rpsA"
                     /locus_tag="ACZPD4_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR1379064.1"
                     /translation="MSELSFEQMLDESFKTIHNGEVVEGTVIDVKPEEIILNIGYKAD
                     GIITKNEYSNDPSVDLTKVVSVGDTMTVKVLKVNDGEGQVLLTYKRLAAEKGNERLRE
                     AFENKEVLKATVTQILGGGICATVDEVRVFIPASLVSDSYEKDLGKYEGQEIEFVISE
                     FNPRRNRVIGDRRQLLVAERAEKQKELFARLQVGDRVDGVIKNVTDFGAFVDLGGVDG
                     LLHISEMSWGRIDNPKKNFHVGDTLTVLVKDIHDTKVALSLKFPETNPWANASENFAV
                     GTVITGKVARMTDFGAFVELAPGVDALLHVSQISREHVEKPSDVLSVGQEITAKIV"
     gene            complement(978..1829)
                     /gene="ispH"
                     /locus_tag="ACZPD4_08170"
     CDS             complement(978..1829)
                     /gene="ispH"
                     /locus_tag="ACZPD4_08170"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR1379065.1"
                     /translation="MKVIKAKTAGFCFGVKRAVDTVYKQVDTKCGQIYTYGPIIHNEE
                     VIKDMRDKGVIVLRSEEDLDMISQGTVIIRSHGVEKRIYEKIEQKGLRIVDATCPFVK
                     KIHNIVSEKSGEGFHIIIIGNPEHPEVVGIKGWAKDNVTVIQSAEDAECFTADRKEKI
                     CIVSQTTFNYNKFKDLVEIIMKKSYDVSVLNTICNATKERQTEAESIAESVDAMIVIG
                     DKHSSNTQKLVEICRKACDNTYYIQTLDDLDLNQLGSVETVGITAGASTPNNIIEEVQ
                     NNVRIIF"
     gene            complement(1845..2501)
                     /gene="cmk"
                     /locus_tag="ACZPD4_08175"
     CDS             complement(1845..2501)
                     /gene="cmk"
                     /locus_tag="ACZPD4_08175"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1379066.1"
                     /translation="MGYNVAVDGPAGAGKSTIAKLVAKEKGYIYVDTGAMYRGLAIHF
                     LKKGISPEDKEAVAKACIDADVSIVYENGVQQVYLNGENITGMLRTEEVGNMASKTSA
                     IPAVREKLLELQKTLAREKDVIMDGRDIGTNILPNADVKIYLTASVETRAKRRYDELA
                     SKGESCDFDEIARDIKERDERDMTREIAPLKKAEDAILVDSSDMTIDEVVSKICSFCN
                     "
     gene            complement(2532..>2717)
                     /locus_tag="ACZPD4_08180"
     CDS             complement(2532..>2717)
                     /locus_tag="ACZPD4_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1379067.1"
                     /translation="IITKGGVSVKEIHPGTMESKFVKNLYFSGEVLDLDALTGGYNLQ
                     IAWSTAYLAGMNAGREE"
BASE COUNT          675 a          697 c          407 g          938 t
ORIGIN      
        1 caacaatctt agctgtaatc tcctgaccta cagaaagaac atccgatggt ttctcaacat
       61 gttctctcga aatctgagat acatgaagaa gcgcatcaac tcccggtgca agttcaacaa
      121 atgcaccgaa atctgtcata cgtgcaactt ttcctgtgat cactgtacct actgcaaagt
      181 tctctgaagc attcgcccac gggtttgtct ccgggaactt caaacttaag gcaacttttg
      241 tatcgtgaat gtctttcaca agaacagtaa gagtatcacc tacgtggaag tttttcttcg
      301 gattatcaat tcttccccat gacatttcag aaatatggag aagtccgtca actccgccaa
      361 gatcaacaaa cgctccgaaa tccgtcacat ttttgatcac gccgtctact ctgtctccga
      421 cttgtaatct tgcaaacagt tctttctgtt tctcggcacg ttctgcaaca agaagctgtc
      481 ttctgtctcc gatcacacgg tttcttctcg gattgaactc gctgattacg aattcaattt
      541 cctgaccttc atattttcca agatctttct cataagaatc agatacaagg cttgccggga
      601 taaacactct gacttcatca actgtagcgc aaatgccgcc tccgagaatc tgtgtaactg
      661 tagctttgag gacttctttg ttctcaaacg cttcacgcag tctctcattt cctttctcgg
      721 ctgcaagtct cttatatgta agaagaacct gaccctctcc gtcgtttact ttcaaaactt
      781 tgactgtcat cgtatcacct acggatacta cttttgtgag gtcaactgac ggatcgttgg
      841 agtattcatt ctttgtaatg ataccgtctg ccttataacc gatattcagg ataatctctt
      901 ccggttttac atcgattacc gttccctcaa ctacttctcc attgtgaatc gttttaaatg
      961 actcgtctaa catctgttca aaagataatt ctgacattat tttgaacctc ctcaattata
     1021 ttgttgggcg tggatgcccc tgctgtaata cctactgttt ccactgaccc taattgattc
     1081 aaatccaaat cgtcaagtgt ctgtatatag tacgtattgt cacatgcttt gcggcagatc
     1141 tcaactaatt tctgagtatt ggaactatgt ttatctccaa taacgatcat agcatccacc
     1201 gactctgcaa tgctttcagc ttcagtctga cgctcttttg tagcattaca gattgtattt
     1261 aaaacactta catcataact ctttttcatt ataatttcaa ctaaatcttt aaatttattg
     1321 taattaaatg tcgtctggga aacaatgcag attttctctt ttcgatcagc tgtaaaacat
     1381 tccgcatctt ccgcactttg tatgacggtt acattgtctt tcgcccatcc ttttatccct
     1441 acaacttcag gatgttccgg gtttccgatt ataataatat gaaatccctc tccgctcttt
     1501 tcagatacaa tattatgaat ctttttcaca aatgggcagg tagcatctac aattctaaga
     1561 cctttttgtt cgatcttttc ataaatgcgt ttttccactc cgtgagaccg gataatgacc
     1621 gtaccttgac tgatcatatc caaatcttct tcagaacgta gtacgataac ccctttatcc
     1681 ctcatatctt tgataacttc ttcattgtgg ataatcggac catacgtata aatctgtcca
     1741 cactttgtat ctacctgctt atataccgtg tcaaccgccc gctttactcc gaaacaaaat
     1801 ccggcagtct ttgcttttat cactttcact gtattttcct ctcactaatt acagaagctg
     1861 catattttac ttaccacttc atcaatcgtc atatccgaac tgtctactaa gatcgcatcc
     1921 tctgcttttt tcaacggtgc aatttctctt gtcatgtcac gttcatctct ttccttgata
     1981 tcccgtgcaa tctcgtcaaa gtcacatgat tccccttttg acgcaagctc atcatatcta
     2041 cgttttgctc ttgtttctac gctggcagtc agatagattt tcacatctgc attcggaaga
     2101 atatttgttc cgatatcccg tccgtccatg atcacatctt tttcgcgtgc cagcgttttc
     2161 tgcaattcta aaagcttttc cctaaccgcc gggatagccg acgtttttga cgccatattt
     2221 ccaacttctt ctgtacgaag catgcctgta atattttcac cgtttaaata aacctgctgt
     2281 actccgtttt cgtacacaat acttacatct gcatctatgc acgcttttgc aacagcctct
     2341 ttgtcttccg gcgaaattcc ttttttcaga aaatggatcg ccaatcctct atacattgct
     2401 cctgtatcta catatatata tcctttttct ttcgccacca gttttgctat cgtacttttt
     2461 ccggctcctg ccgggccgtc caccgccaca ttatatccca ttttctttcc cctttcttac
     2521 tgctttttct tttattcttc tcttccggca ttcattcccg caagatatgc cgtagaccat
     2581 gcaatctgaa gattataacc tcctgtaaga gcatcaagat ccaaaacttc tcccgaaaaa
     2641 tataaatttt taacaaactt tgactccatt gttcccggat ggatctcttt tacgcttact
     2701 ccgccttttg taatgat
//
LOCUS       DBMDXF010000242         2688 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8301_length_2688_cov_1.361036, whole genome shotgun sequence.
ACCESSION   DBMDXF010000242 DBMDXF010000000
VERSION     DBMDXF010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8301_length_2688_cov_1.361036"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="ACZPD4_08185"
     CDS             complement(<1..847)
                     /locus_tag="ACZPD4_08185"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR1379068.1"
                     /translation="MNIYLAPLEGITGWIYRGAVQKCFGGFDKYFIPFIKPNQMGRFS
                     AREKKDILPEHNKGMHAVPQILTNCAEDFVQTAKKLEQYGYKEINLNVGCPSKTVVTK
                     GRGAGFLAFPERLDLFLEEVFQNCPIDISVKTRIGTESGEEFPKLMDIYNKYPIKELI
                     IHPRTQKDFYKNIPHMDVFEKALGTGRHKICYNGDLTSEEDVRNLSEQFPEVESIMIG
                     RGVLADPAIARKIKGGDGADKAEMRHFHDMLYEAYCEEMSGERTVLYKMKELWFYLAS
                     SFTNSR"
     gene            998..>2688
                     /locus_tag="ACZPD4_08190"
     CDS             998..>2688
                     /locus_tag="ACZPD4_08190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1379069.1"
                     /translation="MNTLRKFITYYSPYKTVFFIDLLCAAFISIVDLAYPQILRVLTN
                     TLFTKDSSLILHALPFIAAGLLFMYLIQSLCKYYVSYQGHMMGACMERDMRRQLFDHY
                     EKLSFSYYSKNNSGQMMSKLVSDLFDIAEFAHHGPENLFISVVKIIGSFIFLFLINAR
                     LALPLAIIVLCMFVFSIRQNKKMQETFMDNRRKIGEVNASLQDTLAGIRVVQSFTNEE
                     IERKKFQKSNHAFLLSKKENYHCIGSFMGWNLLFQGLMYSVTLIYGGYLIAKGFMNVG
                     DLAMYALYIGIFISPIQILVELIEMIQKGLAGFRRFLDVMETEPEIKDASDAEELTDV
                     KGLVRYENVSFHYADDDTSVLSHISFEIPAGRSVALVGPSGSGKTTICSLLPRFYDVT
                     GGRITIDGKDVRTLTLKSLRSHIGVVQQDVYLFSGTIRDNIAYGKPDASDEEIIDAAK
                     RANIHDFIMSLDDGYDTMVGERGTRLSGGQKQRIAIARVFLKDPKILILDEATSALDN
                     ESERFIQASLEQLAKNRTTIVIAHRLSTIRHADEILVIDQHQIIERGSHEELLKQ"
BASE COUNT          783 a          622 c          486 g          797 t
ORIGIN      
        1 gtctgctatt agtaaaagag gacgccaaat aaaaccacag ttctttcatc ttatacaaaa
       61 ctgttctttc accggacatt tcctcgcaat atgcttcata tagcatatca tgaaaatgac
      121 gcatttccgc tttatccgca ccatctccgc cttttatctt ccgggcaatc gccggatcgg
      181 cgagaactcc cctgccgatc attatactct ctacttccgg aaactgttcc gaaagattac
      241 ggacatcttc ttccgatgta agatctccgt tataacatat tttatgcctt cctgtgccga
      301 gcgctttttc aaacacatcc atatgcggaa tatttttata aaaatctttt tgtgtacggg
      361 gatgaatgat cagctctttt atcggatact tattataaat atccataagt ttcggaaatt
      421 cctcaccact ttctgttccg atccgcgttt ttacagaaat atcgatcggg caattttgaa
      481 acacttcttc cagaaaaaga tccaaacgtt ccggaaatgc taaaaagccc gctccccgcc
      541 ctttcgtcac aacagtttta gacggacatc ctacattcag attaatctct ttatacccgt
      601 actgttccag cttctttgcc gtctgtacaa agtcctctgc acaatttgtc aatatctgag
      661 gaacagcatg catcccctta ttatgttccg gaagaatatc ttttttctcc ctcgcgctga
      721 aacgtcccat ttgattcggc ttaataaacg gaataaaata cttgtcaaac cctccaaaac
      781 acttctgtac ggcgccgcgg taaatccatc ccgtaattcc ttccaatggg gcaagatata
      841 tattcatata taataactct cctttaatca atatatgacc tttagtaaca gttcatcccg
      901 cgacctttag attattacag cgcccctgcc ttgtcaagaa aaatctcatg tgctaagata
      961 agaacagtat tttttaccaa aaaggagcat aacgtttatg aatacattac ggaaatttat
     1021 cacctattac tcaccgtaca agaccgtctt ttttatcgac cttctttgcg ctgcattcat
     1081 cagcattgtc gatcttgctt acccgcagat cctgcgcgtg ctgacaaata ctttgtttac
     1141 gaaagacagc agtctcatcc ttcatgcgct cccattcatt gcagccggac ttcttttcat
     1201 gtatcttata caaagtcttt gcaaatatta tgtaagttac cagggacata tgatgggcgc
     1261 ctgcatggaa cgtgatatgc gccgacaact atttgatcat tatgaaaaac tttctttttc
     1321 ttattacagc aaaaataatt caggacagat gatgagcaaa ctcgtctccg atctgtttga
     1381 cattgccgaa tttgcacatc acggacctga gaaccttttt atttctgtcg taaagatcat
     1441 cggctctttt attttcctct ttctgatcaa tgcgcggctc gccctgcccc ttgccattat
     1501 tgttttatgt atgttcgttt tttccatacg ccaaaacaaa aaaatgcaag aaacatttat
     1561 ggataaccgg agaaagatcg gagaggtcaa tgcaagtctc caggataccc ttgccggaat
     1621 ccgtgttgta caatctttca caaatgagga aatagaacgg aaaaaattcc agaaaagcaa
     1681 tcacgctttt cttctctcta aaaaagagaa ttatcactgt atcggaagtt ttatgggatg
     1741 gaatcttctg tttcaggggt tgatgtattc agtcactctt atctatggcg gataccttat
     1801 cgcaaaagga ttcatgaacg tcggagacct tgccatgtat gcactttata tcggtatttt
     1861 catcagtccg atccaaatcc tcgtagaact tattgaaatg attcaaaaag gtcttgccgg
     1921 attccgccgt tttcttgacg tcatggaaac agaacctgaa atcaaagacg cttccgacgc
     1981 agaagaatta acagatgtaa aaggcctcgt ccgctatgaa aacgtatctt ttcactatgc
     2041 tgatgacgat acgtctgtcc tttctcacat atcttttgaa attcccgccg gaagatcagt
     2101 cgctcttgtc ggtccgtcgg gaagtggaaa aacaacgatt tgttcccttc tgccacgttt
     2161 ttacgatgtg acaggcggca ggatcacaat agacggaaaa gacgtccgaa cattgacttt
     2221 aaaaagtctt cgaagccata tcggcgttgt acaacaagac gtatatctgt tttcgggaac
     2281 gattcgcgac aacatcgcct acggaaaacc ggacgcgtcg gatgaagaga tcatagatgc
     2341 cgcaaaacgt gcaaatattc acgattttat tatgagcttg gacgatggat atgatacaat
     2401 ggtaggtgaa cgcggaacaa gactttcagg aggacaaaaa caacgcattg ccattgctcg
     2461 cgtattttta aaagacccta aaattttaat tttagacgaa gctacttccg cgctcgataa
     2521 tgaaagtgaa cgatttattc aagcatcttt ggaacagctt gccaaaaaca ggaccacaat
     2581 tgtaattgca catcgtttga gcaccattcg ccatgcagac gaaattttgg taatcgacca
     2641 acatcaaata atagagcgcg gttctcatga agaattgctg aaacaaaa
//
LOCUS       DBMDXF010000243         2648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8450_length_2648_cov_1.482921, whole genome shotgun sequence.
ACCESSION   DBMDXF010000243 DBMDXF010000000
VERSION     DBMDXF010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8450_length_2648_cov_1.482921"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(120..329)
                     /locus_tag="ACZPD4_08195"
     CDS             complement(120..329)
                     /locus_tag="ACZPD4_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HIR1379070.1"
                     /translation="MGKKVLYMAVNFMIRAVIGMALIFFINQYVIPPESSINVGLNAV
                     SFLTSGMLGIPGVGLLYGIVCYQIL"
     gene            complement(593..>2648)
                     /gene="gyrA"
                     /locus_tag="ACZPD4_08200"
     CDS             complement(593..>2648)
                     /gene="gyrA"
                     /locus_tag="ACZPD4_08200"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1379071.1"
                     /translation="NGTSGIAVGMATNIPPHNLREVINAVVKIIDDQIDENGETSIED
                     ILQIIKGPDFPTGAEILGTRGIEEAYRTGRGKIRVRAVTNIEPMQNGKNRIIVTELPF
                     MVNKARLIEKIAELVRDKKVDGITDLSDQSSREGMRICIELRRDVNPNVILNQLYKHT
                     QLQDTFGVIMLALVNNEPKVMNILDMLKYYLKHQEEVVTRRTKYDLNKAEERAHILQG
                     LLIALDNIDEVIKIIRGSKNVQEAKAELISRFDLSEVQAQAIVDMRLRALTGLEREKL
                     EAEYAELMKKIEEYRAILADRKLLLGVIRKEILVISEKYGDERRTHIGYDEFDISMED
                     LIPRENVVITVTNMGYIKRMSMDTFKSQNRGGKGIKGMQTLEEDFIRELFVTTSHHYI
                     MFFTNKGRVYRLKAYEIPEAGRTARGTAVINLLQLMPDEKITATIPIKEYEDGKYLFM
                     ATKKGLVKKTPIQDYMNVRKTGLAAISLREDDLLIEVKVTDNAQDILLVTKYGQCIRF
                     NEKDVRSTGRVSMGVRGMNLSDNDEIIGMQMSSQGKDMLIVSEKGMGKRTSMEEFTKQ
                     NRGGKGVKCYKITEKTGNVVGMKAVDEESEIMIINTEGIVIRMKCSDISQYGRITSGV
                     KLINLPEKERVASVAKVRTASAEIDGEEVEIKEEDDSPENTSEIIVDNSEDNVSDDVE
                     NK"
BASE COUNT          700 a          620 c          366 g          962 t
ORIGIN      
        1 ctgctttccc tcaaaaacag atgttccaac agttgatctc tgtcggtaaa atgcattata
       61 aacagcagat tctattttgt ctacaacaaa ttttaaatct gctgtttttt gtaaaaaact
      121 cacaaaatct gataacaaac aatcccataa agcagtccaa ccccaggaat tccaagcatt
      181 cccgatgtca aaaaagagac tgcattcaaa cctacattaa ttgaagattc aggtggaata
      241 acgtattgat ttataaaaaa aataagcgcc attcctatca ccgcacggat cataaaattc
      301 actgccatgt acaaaacttt ctttcccagt tgtcccgctc cctttctttt tacgtattac
      361 ctttttactt taatactttt tataaattat tttattctat atcatcaccc attattacat
      421 cgtattctta atagaacatc atccgtactt ctgttcgcag cgccgcaaaa aaagaccgac
      481 aatgaacttt ttttcaatct atttatcgca aacttctaac gattgaaaaa tcagttcatt
      541 atcgatcttt tcacacagat taaactattt atcaacaatg ttttctctgt ttttatttgt
      601 tttccacatc atctgatacg ttatcttctg aattatccac tataatttca gatgtatttt
      661 ccggcgagtc gtcctcttct ttgatttcaa cttcttcacc gtcaatttcc gctgaagctg
      721 ttcttacttt tgctacactt gccactcttt ctttctcagg aagattaatc aattttactc
      781 ctgatgttat tcttccatat tgagaaatat cagaacattt cattcggatt acgattcctt
      841 ctgtattgat gatcatgatc tcgctttctt catctacagc tttcataccg actacatttc
      901 cggtcttttc cgtaatctta tagcatttca cgccttttcc gccgcgattt tgttttgtga
      961 attcttccat acttgtacgt tttcccatac ctttttccga aacaatcagc atatctttgc
     1021 cctgactgct catctgcata ccgatgattt catcattatc gctcaaattc attcctctga
     1081 cacccataga aacacggcct gtggaacgaa cgtctttttc gttgaaccgg atacactgtc
     1141 catattttgt aacaagaaga atatcctgtg cattatctgt tacttttact tcgattaaaa
     1201 gatcgtcttc ccttaatgag attgccgcaa gtccggtctt tctcacgttc atataatctt
     1261 ggatcggagt ctttttaaca agacctttct ttgttgccat aaacagatac tttccgtctt
     1321 catattcttt gatcggaatt gtcgccgtaa ttttttcatc cggcataagc tgaagtaaat
     1381 taataacagc cgttcctctt gccgttcttc cagcttccgg tatctcatat gctttcagcc
     1441 tgtacacacg acctttattt gtgaagaaca taatataatg atggctcgtt gtaacaaata
     1501 attctcttat aaaatcttct tcaagcgtct gcattccttt aattcctttg ccacctctgt
     1561 tctggctctt gaacgtatcc atgctcatcc gcttaatata tcccatattt gtcacagtaa
     1621 tgacaacatt ttctctcgga atcaaatcct ccatcgaaat gtcaaattca tcatagccga
     1681 tatgagttct tctttcatct ccgtattttt cggaaataac aagaatttct tttcttataa
     1741 ctccgagaag aagttttctg tccgcaagaa ttgccctata ttcttcgatt tttttcatca
     1801 attcagcata ttccgcttcc agtttttctc tttccaaacc tgtaagagca cgcagacgca
     1861 tatccacgat tgcctgtgcc tgcacttctg aaagatcaaa tcgagagatc aactctgctt
     1921 ttgcttcctg aacatttttc gatccacgaa taatcttgat tacttcgtca atattatcta
     1981 aggcaatgag caatccctgc aaaatatgag ctctttcttc cgccttattc agatcgtatt
     2041 ttgttcttct tgtaacaact tcttcctgat gctttagata atatttcagc atatcaagaa
     2101 tgttcatgac tttcggctca ttatttacaa gtgcaagcat gatcactccg aacgtatcct
     2161 gaagctgtgt atgtttataa agttgattta gaataacatt tggatttaca tcacggcgaa
     2221 gttcgataca gatacgcatt ccttccctgc ttgactgatc gcttaaatcc gtaattccat
     2281 ctactttttt gtctcttaca agttctgcta ttttttcaat aagacgtgcc ttatttacca
     2341 taaacggaag ttctgtaacg ataattctgt tttttccgtt ctgcatcggc tcgatatttg
     2401 tcacagctct tactctgatt tttccgcggc ctgtcctata tgcttcttct attcctctcg
     2461 taccgagtat ctctgcccct gtagggaaat caggtccttt gataatttga agaatatctt
     2521 caatcgaagt ttctccgttt tcatcaatct gatcgtctat gatctttaca acggcattga
     2581 tgacttcacg cagattatgc ggaggaatat tggttgccat accaaccgcg atacctgacg
     2641 taccattt
//
LOCUS       DBMDXF010000244         2614 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8577_length_2614_cov_1.612415, whole genome shotgun sequence.
ACCESSION   DBMDXF010000244 DBMDXF010000000
VERSION     DBMDXF010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_2614_cov_1.612415"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="ACZPD4_08205"
     CDS             <1..453
                     /locus_tag="ACZPD4_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379072.1"
                     /translation="SRNYGGHTKMKTENFCKENRPEVKKKLRLPDICEQIDFFEEVPE
                     YDNELYRHKKMKTDPEKGLVLLREVLPVLEAQEAFDNDSLFETLKSFAGEKEYKIGYV
                     MWPLRTAVSGKQNTPGGATELMEVLGKEESLKRIRAGIEKLSQTAEAE"
     gene            478..2304
                     /locus_tag="ACZPD4_08210"
     CDS             478..2304
                     /locus_tag="ACZPD4_08210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1379073.1"
                     /translation="MGFNKAQNEAIMHGKGPCQVLAGPGSGKTLTIVNRIKYLIERYE
                     VRPEEILVVTFTRFAAAEMKSRLCSLMGKSNVPVTAGTFHGIYYGILKWAYRLGPDNI
                     LSEEEKYRILRRVSEDCGTEAAEEEDFLHDIAEEIGLVKNNGLKIGQFVSKKCRQDTF
                     RELYEKYEAQRKAVRKLDFDDMLVLCKDLFVSHPDVLKQWQTKFKYILVDEFQDINRV
                     QYDVIRMLALPENNLFVVGDDDQAIYGFRGADSEFMFRFSEDYPQAKRIILNINYRSS
                     GNIVKNSMKVIGHNEKRFLKEFQSVKENGFCVHVQEVKDPNEEAQYVADEIEKRAELG
                     ISYEEMAVLFRIHTDARPVAEEFLERKIPFQMKERLPNLYDHFIAKDICAYFRLALGN
                     IERNDLLQVMNRPKRYIGRDSVASGKPSFEEMRKFYCDKEWMLDRIDQFEWDVKMLRK
                     TAPYAGINYIRKRIGYDDFLKDYAFTRKIDRANLNEVLAELEEAARPFATIEEWFRHI
                     EEYTRLLRLKSMKRQEDREGVRLMTLHSAKGLEFDTVFLVEANEGKMPYKKAKTDAET
                     EEERRLFYVGMTRAKEVLKVSYVKTKNGKETSPSRFVDELLE"
     gene            complement(2324..>2614)
                     /locus_tag="ACZPD4_08215"
     CDS             complement(2324..>2614)
                     /locus_tag="ACZPD4_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1379074.1"
                     /translation="ITVTLTLENDEELECAVLSIFEAEEREYIALLPLDENGDNDDGQ
                     VFLYRFIDNGEDAEPDLENIEDDQEFERVSNAFNELMEEQDFADIDLDELED"
BASE COUNT          851 a          437 c          665 g          661 t
ORIGIN      
        1 tcccgaaatt acggaggaca tacgaaaatg aaaacagaga atttctgcaa agagaaccgt
       61 cccgaagtga agaagaaatt gcgcctgccg gatatttgcg aacaaattga cttctttgag
      121 gaagttccgg agtatgataa cgaattatac agacataaga agatgaaaac ggatcctgag
      181 aaaggactcg tgcttttgcg ggaagtgctt ccggttcttg aagcgcagga agcgtttgac
      241 aatgattctt tgtttgagac actcaaaagt tttgcgggag agaaagaata taagatcggt
      301 tatgttatgt ggccgcttcg tactgcagta tcaggcaaac agaacacacc gggcggagca
      361 acagaactga tggaagttct cggaaaggaa gaatctctta aaagaattcg tgcagggatc
      421 gagaaactca gccagactgc agaggcagaa taaacatcgg tcaaagcgag gaatcagatg
      481 ggatttaata aggcacaaaa tgaagcgatc atgcacggaa aaggaccgtg tcaggtgttg
      541 gcgggaccgg gatccggaaa aacgctgacg atcgtaaaca gaattaaata tttgatcgaa
      601 agatatgaag taagaccgga agagattctg gtcgttactt ttacccgttt tgcggcggca
      661 gagatgaaat ccagactttg cagcctgatg ggaaaatcaa atgttccggt tactgccgga
      721 acgtttcatg gcatttatta cggcatttta aagtgggcat acaggcttgg accggataat
      781 attttgtcag aagaagaaaa atatcgaatt cttcgaagag tatcggaaga ttgcggaaca
      841 gaggcggcag aggaagaaga ttttctgcat gatattgcgg aagaaatcgg acttgtaaaa
      901 aataacgggc tgaaaatagg acagtttgtt tccaagaaat gcagacagga tacattcaga
      961 gaattatatg aaaaatacga agctcaaaga aaagctgtca ggaagctgga ctttgatgat
     1021 atgcttgtgt tgtgcaaaga cttgtttgta agccatcctg atgtattgaa acaatggcag
     1081 acaaagttca agtatatatt agttgatgag tttcaggata tcaacagagt tcaatacgat
     1141 gtgattcgta tgctggctct tccggaaaat aatctttttg tagtcggaga cgacgatcag
     1201 gcaatttacg gcttccgcgg agctgattcg gaatttatgt ttcgattttc ggaagattac
     1261 cctcaggcaa aaaggatcat attgaatata aattataggt catcgggaaa tattgtaaaa
     1321 aactcgatga aggtgatagg acataatgaa aagaggtttc taaaagagtt tcagtctgta
     1381 aaggaaaacg gattctgcgt gcatgtgcag gaagtgaaag acccgaacga agaggcgcag
     1441 tacgttgcgg acgagataga aaaacgtgca gaactgggga tttcgtatga agagatggca
     1501 gtccttttcc gcattcatac agatgcgcgt ccggttgccg aagaatttct ggaacggaag
     1561 atcccgtttc agatgaaaga gcggcttccg aatctatacg accattttat cgcaaaagat
     1621 atttgcgcct atttcaggct ggctcttgga aatatagaac gaaatgatct tttgcaagtt
     1681 atgaaccgcc cgaaacgata tataggaaga gacagtgttg catcagggaa accttctttt
     1741 gaagaaatga gaaaatttta ttgcgataaa gaatggatgc tggatcggat cgatcaattt
     1801 gaatgggatg taaaaatgct tcgaaaaaca gcgccatatg cgggaattaa ttatatacga
     1861 aagcggatcg gatatgatga ttttttgaaa gattatgcgt ttacgagaaa aatcgatcga
     1921 gcgaatctca atgaagtgct ggcagagctg gaggaggctg cgcgcccttt tgcaacgata
     1981 gaagagtggt ttcgtcatat agaagaatat acgcgtttgc tccggctaaa gtcgatgaag
     2041 agacaggaag acagagaagg ggttcgtctt atgacactgc attctgccaa aggacttgag
     2101 tttgatactg tatttctcgt agaagcaaac gaggggaaga tgccatataa gaaagcaaag
     2161 acggatgctg agacagagga ggaaagaagg ctgttttatg tcggtatgac gagggcaaaa
     2221 gaagtgttga aagtatctta tgtaaaaaca aaaaacggga aagagacaag tccttcccgt
     2281 tttgtagatg aattattaga atagggatga gcggatgaaa agatcagtcc tcaagctcgt
     2341 cgagatctat atccgcaaaa tcctgttctt ccatcaactc attaaatgca tttgaaacac
     2401 gttcaaattc ctgatcgtct tcgatgtttt ccagatccgg ctctgcgtct tcgccattgt
     2461 cgatgaaacg ataaagaaat acttgtccat cgtcattgtc tccgttttcg tcaagcggaa
     2521 gaagggcgat gtattctctt tcttcggctt caaagatgga aagaacggca cattccagtt
     2581 cttcatcatt ttcaagtgtc agtgtaactg tgat
//
LOCUS       DBMDXF010000245         2610 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8584_length_2610_cov_1.912714, whole genome shotgun sequence.
ACCESSION   DBMDXF010000245 DBMDXF010000000
VERSION     DBMDXF010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8584_length_2610_cov_1.912714"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            236..1156
                     /locus_tag="ACZPD4_08220"
     CDS             236..1156
                     /locus_tag="ACZPD4_08220"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1379075.1"
                     /translation="MKKPVLVIMAAGMGSRYGGLKQIDPIDKEGHIIMDFSIYDAKRA
                     GFEKVVFIIKKENETDFKEAVGKRIEKVMDVAYVYQELTNIPDGFEIPKDRVKPWGTA
                     HAVLSAIDEIDGPFAVINADDYYGRHAFQTIYDYLTTHEDDDKYRYTMVAYRLKNTVT
                     DNGHVARGICGLNASNELVSVTERTRIEKRNNGIEYSEDDGQTWNEIDPDTLVSMNMW
                     GFTRSILEEIKTGFPAFLEKGLKENPLKCEYFLPAVVSDLLAEDRAAVKVLESEDKWH
                     GVTYKEDKPVVVEAIQSLKDSGLYPQHLWD"
     gene            1319..2335
                     /gene="galE"
                     /locus_tag="ACZPD4_08225"
     CDS             1319..2335
                     /gene="galE"
                     /locus_tag="ACZPD4_08225"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1379076.1"
                     /translation="MSILVTGGAGFIGSHTCVELLNAGYDVVIVDNLYNASEKAVDRV
                     KEITGKDLKFYQVDIRDREGLNEVFDKEDVESVIHFAGLKAVGESVQKPLEYYENNIG
                     GTITLCDVMRSHGVKNIIFSSSATVYGDPAFIPITEECPKGVCTNPYGWTKWMLEQIL
                     TDLHTADPEWNVVLLRYFNPIGAHKSGMIGEDPKGIPNNLMPYITQVAIGKLECLSVF
                     GDDYDTHDGTGVRDYIHVVDLAIGHVRAVEKLKEKDGVSVYNLGTGNGQSVLDMVKAF
                     GKACGKEIPYVIKPRRAGDIATCYCDASKAKKELHWEAERGLEEMCEDSWRWQSQNPN
                     GYAE"
BASE COUNT          898 a          393 c          659 g          660 t
ORIGIN      
        1 aacgtcatgc tttccgaatg tatttttata gtttaaagaa atctgcccgt taggtttgaa
       61 ataattgtca gattgtgttg tcaattcgga tatagtgtga gaaaaaagaa aaagcagaca
      121 aagaaaaaat ccggaatgaa ataacgtcag gtggacgttc tttgatgaat gtggtatcct
      181 tatttcagat gacagaaaat gataagatta agatttaagt atgaggtgat ttaagatgaa
      241 gaaaccggta ttggtgatta tggcagcggg aatgggaagc agatacggcg gattgaaaca
      301 gatcgatccg atcgataaag aaggtcatat tattatggat ttttctattt atgatgcaaa
      361 gcgcgccgga ttcgagaaag tcgtttttat tattaaaaag gaaaacgaga cggactttaa
      421 agaagcagtc ggaaaacgga ttgagaaagt aatggatgta gcttatgtgt accaggaact
      481 gacaaacatt ccggacggat ttgagattcc gaaagaccgt gtaaaaccgt ggggaacagc
      541 tcatgcggtg ttaagtgcga tcgatgagat cgacggaccg tttgctgtga tcaatgcaga
      601 tgattattac ggacggcatg cgtttcagac aatttacgat tatttgacga cccatgaaga
      661 cgacgacaag tatcggtata cgatggttgc ataccgtttg aaaaatactg tgacagataa
      721 cggacatgtg gcaagaggta tctgcgggtt aaacgcatcg aatgaacttg taagcgttac
      781 ggaaagaacc cgtattgaaa agagaaataa cggcattgaa tattcggaag atgacggaca
      841 gacatggaac gaaatcgatc cggatacact tgtatcaatg aatatgtggg gatttacgag
      901 aagtattcta gaagagatca aaacgggatt tccggcattt ttagagaaag gattgaaaga
      961 aaatccttta aaatgtgaat attttctccc ggcggttgta agtgatcttt tagcagaaga
     1021 ccgcgctgca gtgaaagtac ttgagtccga agataagtgg cacggagtta cttataaaga
     1081 agataagccg gtcgtcgtag aagcgataca gtctctgaaa gattcgggct tatatccgca
     1141 gcatttgtgg gattagtctt aatgcaacat ttttactata agatgatata aaaatataaa
     1201 gctattttgt aggatgagtg gtaagatgtg catgtaaaat aaaacggcag taaaaaggaa
     1261 aactgcaata gacagaacct tgaacggtat ctgaaatatt tacgaggagg aaacagatat
     1321 gtcaatctta gtaacaggcg gcgccggatt tatcggcagc catacatgtg tggaattgtt
     1381 aaatgcaggg tatgatgtgg tcatcgtaga taatttatat aacgcatcgg aaaaagcggt
     1441 agatcgcgtg aaagagatca caggaaaaga tctgaaattt tatcaagtag atatccgcga
     1501 cagagaaggg cttaatgaag tgttcgacaa agaagatgta gagtcggtaa ttcattttgc
     1561 cggacttaaa gcggtagggg agtctgtgca gaagcctctc gaatattatg aaaacaatat
     1621 cggaggaacg atcactcttt gtgatgtgat gagaagtcat ggagtaaaaa acattatttt
     1681 cagttcttcg gcaacagtat acggggatcc ggcatttatt ccgatcacgg aagagtgtcc
     1741 aaagggtgtg tgtacaaatc catatggatg gacaaaatgg atgttagagc agattttgac
     1801 agatcttcat acggcagatc cggagtggaa cgtagtgctt cttcgctatt tcaatccgat
     1861 cggagcgcat aaaagcggta tgatcggaga agatccgaaa ggaattccga ataacctgat
     1921 gccatatatt acacaggtgg cgatcggaaa actggagtgt cttagcgtat ttggagacga
     1981 ttatgacaca catgacggaa caggggtgcg cgattatatc catgtagtcg atcttgcgat
     2041 aggtcatgtg cgcgcagtag agaagctgaa ggaaaaagac ggtgtttcgg tatataatct
     2101 cggtacagga aacggacaat ccgtattaga tatggtaaaa gcattcggaa aagcgtgcgg
     2161 aaaagagatt ccatatgtaa ttaaaccgcg ccgtgcaggc gatattgcaa catgctactg
     2221 cgatgcgtcc aaagcaaaaa aggaacttca ctgggaagca gagcgtggat tagaagaaat
     2281 gtgtgaagat tcgtggagat ggcagagcca gaatccgaat ggatatgcag agtgaaaata
     2341 agcatgataa tcaacagaaa atagaaataa caagttagaa ataacaagta tgtccgcggg
     2401 aaatttttct tttctgcgga catacttgca tgtaggcaaa gaaaagcatt aaaaatacgt
     2461 tttcttctga tctgataaaa tgcatttaaa atccgtacaa caattttgca aatcccattt
     2521 catggaagaa aaagaaacgt ccggcaaaga ggatgaaaat gtagagcgga agttccaaga
     2581 caggataaga aaaatcattt aacttattat
//
LOCUS       DBMDXF010000246         2609 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8590_length_2609_cov_1.424242, whole genome shotgun sequence.
ACCESSION   DBMDXF010000246 DBMDXF010000000
VERSION     DBMDXF010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8590_length_2609_cov_1.424242"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="dnaN"
                     /locus_tag="ACZPD4_08230"
     CDS             complement(<1..916)
                     /gene="dnaN"
                     /locus_tag="ACZPD4_08230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1379077.1"
                     /translation="MKIICTKQNLAKGVSIVSKAVPSKTTMPILECILIDASTDVIKL
                     TANDMELGIETSIDGEIIERGILALNAKIFAEIVRKLPDNEIIIDTQGDNQALITGGK
                     AKFKIASLPGDEFSYLPNIEKDDFITVSEFTLKEVIRQTIFSIADSDTNKMMTGELFE
                     IEDNILKVVSLDGHRISIRKIELKDSYAPKKVIVPGKTLQEISKIIGGEAEADVDISF
                     TKNHIVFEFDRTIVVSRLIEGEYFRIDQMLSSDYETKVKINKRELLECIDRATLLIKE
                     GDKKPLIIDIRDESMEFKIKSQVGSMDED"
     assembly_gap    974..983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1179..2549)
                     /gene="dnaA"
                     /locus_tag="ACZPD4_08235"
     CDS             complement(1179..2549)
                     /gene="dnaA"
                     /locus_tag="ACZPD4_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR1379078.1"
                     /translation="MNIVEQNWDLILNKMKLEYCSSNISYTTWIAPLTVYEVKDNTVY
                     ILVQLRASLEHIEEKYLLPFKVCIAEVTGIEFEVAFVTDNEAVIQEKKENIIKKNRVN
                     AIYEKANLNPKYTFDTFVVGSNNNFAHAASLAVAESPGEIYNPLFIYGGVGLGKTHLM
                     HSVAHFILANDPSKKVLYVTSETFTNELIDALKAGKTGSELAMTKFREKYRNNDVLLI
                     DDIQFIIGKESTQEEFFHTFNHLHVSGKQIIISSDKPPKDIETLEARLRTRFEWGLIA
                     DISSPDYETRMAILRKKEELDGLEKYHIPDDVMQYIANNIKSNIRELEGSLNKLIALS
                     NLENKKIDIPLAAEALKDMISPDNNREVTPELIIDIVSEHFNIPISELKGKKRNAEIV
                     LPRQIIMYLCRTLTDTPLKAIGAILGGKDHASVNHGVKKIENELKTDEALNNTVNIIK
                     KKINPI"
BASE COUNT          725 a          497 c          459 g          918 t
ORIGIN      
        1 tatcttcatc cattgaaccg acctgtgatt ttattttaaa ttccattgat tcgtctctga
       61 tatcgataat aagcggtttt ttgtctcctt ctttgataag caaggtagcg cggtcgatac
      121 attcaagaag ctctctttta tttattttta ctttcgtttc ataatcgctt gagagcatct
      181 gatctatacg gaaatattct ccttctatca gtcttgatac tacgatcgtc ctgtcgaatt
      241 caaatacgat atgatttttc gtaaatgaaa tatctacatc ggcttctgct tccccgccta
      301 tgatcttact gatttcctga agtgtttttc cgggaacgat taccttttta ggcgcataag
      361 aatctttcag ttctattttt ctgattgaaa ttctatgacc gtcaagtgat acgactttca
      421 aaatattatc ttcgatttca aaaagttctc ctgtcatcat tttattcgta tcgctgtcgg
      481 cgatcgaaaa gatcgtctga cggattactt cttttagtgt aaactccgac actgttataa
      541 aatcatcttt ctcaatgttc ggaagataag aaaattcatc tcccggaagt gatgcgattt
      601 taaattttgc ttttccacct gtgataagcg cctgattatc gccttgtgta tctatgataa
      661 tttcgttgtc gggaagtttc ctgacgatct ctgcaaatat ttttgcgtta agagccaaaa
      721 tacctctttc aatgatctct ccatctatag acgtttcgat tccaagttcc atatcgttag
      781 ctgtaagttt aatgacgtct gtagatgcgt ctattaaaat acattcgaga attggcatcg
      841 ttgtttttga cggaacagct ttggaaacga tactcactcc ttttgccaga ttctgttttg
      901 tgcatatgat tttcataaaa aagtaactcc tttcgatctg tgtggaaaag tcagacgcgc
      961 acgaaataat atannnnnnn nnnagaggct gtgaataagt tgaaaaccat ctcaacccct
     1021 tgaaaaatca aggttttctg cttgtgataa ctgtgtggga ttcgagttga aacgatgagg
     1081 aaagtgtggg tataaatctg aacctcttca ggtcgatatt atgttattca catcttttcc
     1141 catagttttt aagaagtaat ctacatagtt atccacaatt atatcggatt tatcttcttt
     1201 tttataatgt tgacggtatt attaagagct tcatcggttt ttaattcgtt ttcgatcttc
     1261 ttaacgccgt gattgacaga agcatggtct tttccgccta agatagcgcc aatcgcctta
     1321 agaggcgtat ccgtcagagt acggcaaaga tacatgatga tctgtcttgg aaggacgatt
     1381 tctgcatttc gcttctttcc ttttaactct gaaatcggaa tattaaagtg ttcagataca
     1441 atatcaatga taagttcagg cgtcacctct cggttattat ccggagagat catatccttt
     1501 aacgcctccg ccgcaagcgg gatatcgatc tttttatttt caaggttaga aagagcgatc
     1561 agcttattaa gagagccttc caattcacga atattcgatt tgatattatt ggcaatatat
     1621 tgcataacat cgtccggaat atggtatttt tcaagtccgt caagttcttc tttttttctt
     1681 agaattgcca ttcttgtctc ataatcaggc gacgagatat cggcaataag tccccactca
     1741 aatcttgtcc taagacgggc ttcaagtgtc tcgatatctt tcgggggttt atcacttgat
     1801 atgataatct gttttcccga tacatgaaga tgattaaaag tatggaagaa ttcttcctgt
     1861 gtactttctt ttcctattat aaactgaata tcgtcgatta aaagaacgtc gttatttctg
     1921 tacttttcac ggaattttgt catggcaagt tcacttcccg ttttaccggc tttcaaagcg
     1981 tcgatcaatt cattcgtgaa agtttcactt gtaacgtaca atactttttt agacggatcg
     2041 tttgcaagta taaaatgagc gacggaatgc ataagatgag ttttacctaa tccgacgcct
     2101 ccgtatataa agagaggatt gtaaatctct ccgggtgatt ccgcaactgc aagtgaagcg
     2161 gcatgcgcga aattgttgtt acttccgact acgaaagtat caaatgtata ttttggattc
     2221 aggttcgctt tttcataaat cgcgttaaca cggtttttct taataatatt ttcctttttt
     2281 tcctgaataa ccgcttcatt gtctgtaaca aaagcaactt caaattcaat tccggttact
     2341 tccgcaatgc agactttaaa aggaagaaga tatttttctt cgatatgttc aaggcttgcc
     2401 cttagttgga cgagaatata aacggtattg tcctttactt cataaactgt caaaggcgca
     2461 atccaagtag tataagaaat attagatgag cagtattcaa gtttcatctt atttaatatc
     2521 aaatcccagt tttgttctac aatattcatc aaaagtaact cctaatcaat agaatagaga
     2581 gatataaagg cagaatctct ctattacat
//
LOCUS       DBMDXF010000247         2602 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8610_length_2602_cov_2.695322, whole genome shotgun sequence.
ACCESSION   DBMDXF010000247 DBMDXF010000000
VERSION     DBMDXF010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8610_length_2602_cov_2.695322"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..>960
                     /locus_tag="ACZPD4_08240"
     CDS             2..>960
                     /locus_tag="ACZPD4_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1379079.1"
                     /translation="MDLLHKKNVEVSNHMSSIEDNIADEIRKEKNTRSEGKSEGEEAP
                     KKKNLAFVIRPQNSKNSSRIQGKRPAQKSARPQQSARQNEAKAATTRPASQGARSEKS
                     GAEKSQAARPVPAQTVKAETAAEHSTTEKRPGTQAQSAAEKPQGVRQEKQQPARAEKP
                     VQVQSEKEKRTENDRPARENREARQSNDRPVRENRDGKPVRDGKPSDRQGRFDRGSND
                     RSRQERPQGNRQFGDGRQSRGGDRDRRPGGQNGRSDREKRESRRDDVAAPIVEQQKSQ
                     RNKAKDKERDNKKKEYRDKRTGMQTVRSAIFAIVLIITGFISI"
     assembly_gap    961..970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <971..>2602
                     /gene="infB"
                     /locus_tag="ACZPD4_08245"
     CDS             <971..>2602
                     /gene="infB"
                     /locus_tag="ACZPD4_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1379080.1"
                     /translation="KKKEYRDEAFVGKGKKGKKQKQVAEVKRPQPKEEKKEEKIKQIV
                     IPEVLTIKELADKMKIVPSVIVKKLFLQGKVVTVNQEIDYDTAEEIAMEFEVLCEKEE
                     VVDVIEELLKEEEEDENMMVKRPPVVCVMGHVDHGKTSLLDAIRHSNVIDREAGGITQ
                     HIGAYVVEANGEKITFLDTPGHEAFTAMRMRGASSTDIAILVVAADDGVMPQTVEAIN
                     HAKAAGIEIIVAINKIDKPSANIERVKQELTEYELIPEDWGGSTIFVPVSAHTKEGIP
                     ELLEMVLLTAEVMELKANPNRAARGLVIEAELDKGKGSVATVLVQKGTLHVGDAIAAG
                     SAHGRVRAMMDDKGRRVKEAGPSQPVEILGLNDVPNAGEVFVGCDSEKDARSFAETFI
                     AQNKVKLLDETKSRMSLDDLFSQIQEGNLKELNIIVKADVQGSVEAIKQSLLKLSNDE
                     VVIKIIHGGVGAINESDVSLASASNAIIIGFNVRPDATAKEIADREGVDLRLYRVIYN
                     AIEDVEAAMKGMLDPVFEEKILGHAEVRQTFKASGV"
BASE COUNT          909 a          468 c          694 g          521 t
ORIGIN      
        1 catcgatttg ttgcataaga aaaatgtgga ggtgtcaaat catatgagtt cgatcgagga
       61 caatatagca gatgagatcc gaaaagaaaa aaacacaaga tcagaaggaa agtcagaggg
      121 agaagaagct ccgaaaaaga aaaatctggc atttgtgatt cgtccgcaaa actctaaaaa
      181 cagcagtcgc atacagggaa aacgtccggc tcaaaagtct gcgcgtccac agcagagtgc
      241 gcgtcaaaac gaggcaaagg cagcgacgac gcgtccggca tcacagggag cacgttcgga
      301 aaagtcaggt gcagaaaagt cacaggctgc gcggccggtt cctgctcaaa ctgtaaaagc
      361 cgaaactgct gccgaacatt caactacaga aaaacgaccg ggtacgcaag cgcagtcagc
      421 agcggaaaag ccgcagggag tacgtcagga aaagcaacag ccggcacgtg cggaaaaacc
      481 ggtacaggta cagtccgaaa aggagaaaag aacagagaat gaccgtccgg caagagaaaa
      541 tcgtgaagca agacagtcca atgatcgtcc ggtaagagaa aacagagacg gaaagccggt
      601 aagagacgga aaaccgtctg acagacaagg cagattcgat cgcggatcca atgatcgttc
      661 gcgccaggaa agaccacagg gaaatcgtca gttcggtgac ggaagacaga gccgaggcgg
      721 agacagagac cgcagaccgg gaggacaaaa tggtcgatct gacagagaga aaagagagtc
      781 acgaagagat gatgtggcgg caccgatcgt ggagcagcag aagagccagc gtaataaggc
      841 gaaagataaa gaacgtgata ataagaaaaa agaatacaga gacaaaagaa caggcatgca
      901 gactgtaagg tctgccatat ttgctattgt gcttataatt acaggtttta tcagtataat
      961 nnnnnnnnnn taagaaaaaa gaatacagag acgaagcctt tgtcggaaaa ggcaaaaaag
     1021 gcaaaaaaca aaaacaggta gcagaagtga aaagaccgca gccgaaagaa gagaagaaag
     1081 aagaaaagat taaacagatt gtaattccgg aagttcttac gatcaaagaa cttgcagata
     1141 aaatgaagat tgttccttct gttatcgtga aaaaactgtt ccttcaagga aaagttgtaa
     1201 cagtgaatca ggaaatcgat tatgatactg cagaagagat cgcaatggaa tttgaagttc
     1261 tctgcgaaaa agaagaagtc gtagatgtga tcgaagaact gttaaaagag gaagaggaag
     1321 atgaaaatat gatggtaaaa cgtcctccgg tcgtatgtgt tatgggacac gttgaccatg
     1381 gtaaaacatc tcttcttgat gcgatccgtc actcaaatgt gatcgatcgt gaagcaggtg
     1441 gtatcactca gcatatcgga gcatatgttg tggaagcaaa cggagaaaaa attacgttcc
     1501 tcgacacgcc gggacatgag gcgtttacgg caatgcgtat gcgcggtgca agctctacgg
     1561 atatcgccat tcttgtcgta gcggcagacg acggagtaat gcctcagaca gtagaagcaa
     1621 tcaatcatgc aaaagctgcc ggaattgaaa ttattgttgc aatcaataag attgataaac
     1681 caagtgcaaa tatcgaacgt gtgaaacaag aactgacaga atatgagttg attccggaag
     1741 attggggcgg aagtacgatt tttgttcctg tatccgcaca tacaaaagaa ggaattccgg
     1801 agcttcttga aatggttctt ctgacagcag aagttatgga gttaaaagca aatccgaacc
     1861 gtgcggcaag aggtcttgtg atcgaggcgg aacttgataa aggaaaagga tctgttgcga
     1921 cagttcttgt tcagaaaggt actcttcatg tcggagatgc aatcgcggca ggaagcgcac
     1981 atggtcgcgt tcgtgcgatg atggacgata aaggcagaag ggtgaaagag gcaggtccgt
     2041 cacagccggt agagattctc ggattaaatg atgttccaaa tgcgggagaa gtgtttgtcg
     2101 gatgtgattc cgaaaaggat gcaagaagtt ttgcagagac gtttatcgct cagaataaag
     2161 tgaaacttct tgatgagaca aaatcaagaa tgtctcttga tgatctgttc agccagatac
     2221 aggaaggcaa tttgaaagaa ctcaacatta tcgtaaaagc agatgttcaa ggttcagtag
     2281 aagcaatcaa acagagtctt ttgaaacttt caaatgatga agttgtaatc aagatcattc
     2341 acggtggagt aggcgcaatc aatgaatcgg acgtcagcct tgcatcggct tccaatgcga
     2401 tcattatcgg atttaatgta cgtccggatg cgacggcaaa agaaatcgcc gatagagaag
     2461 gcgttgattt aagactttac agagtcattt ataatgcgat agaagatgtg gaagctgcaa
     2521 tgaaaggaat gcttgatccg gtatttgagg aaaaaattct cggtcacgcg gaagtgcgtc
     2581 agacatttaa agcatccgga gt
//
LOCUS       DBMDXF010000248         2595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8633_length_2595_cov_1.912590, whole genome shotgun sequence.
ACCESSION   DBMDXF010000248 DBMDXF010000000
VERSION     DBMDXF010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_2595_cov_1.912590"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2337)
                     /locus_tag="ACZPD4_08250"
     CDS             complement(<1..2337)
                     /locus_tag="ACZPD4_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HIR1379081.1"
                     /translation="MKMKQTFALLCAGAMVITGSAFPVNVQASADWVWNEEVSESGYK
                     KVPYTELTTTADTEADNESSTGEGAASPGTGHAVNATDGVITSYYHSCYSGDNQPNRE
                     DGALTGNNTITVELQNAQSIQGITYLPRQDAFGNGTVTEFSVSVQKDGTENWENVGNN
                     LHISYNNPGANWSAENKSEKEFVFDAEVENVKKVKLTVIHTDGSAPNQHISAAEISIL
                     TPVKKDAETEALKIRIREKLAQMESQMELNVLSGNADSGKVYSRAKFDPIKAEAEAKL
                     AQESTTKEELEAVFQQLEQFKMEFQDELGTGVYVSDLTHLSGNTAWGGVKKDLCPDGN
                     RLIAIKKDTSTINTYTKGIGAHEPSSIVYDVTGYKMFSADIGVENHQIASDGGVFANV
                     KAKFKVSTSTDGSNYTQQYYAEDLSANGGLRSIRVKLPEGTTHMKLECPASAWNNGNG
                     GKLHTCWANAKLYETVEIAVTGITLDQDALALKVGGTEEQKTATLQAAITPEDATDQE
                     VLWESDNEEVATVDGGVVTAHKAGNATIKVSSVNYPNITAQCIVTVTRDDTALEEAIA
                     AAESRMKEENFAEKYTEATRKALEDDLADAKAAKDNTALSVEEVKLIVESLKTSIEGL
                     QLKAVVTINNNGNTETKYCEVGDQVRVVAAQAPEGKKFSHWTVNEKPICYNESYTFTV
                     YKDIAVTSVYVEEAEEIQKEVSVLCDVSYANGRVKFLSKYSVPTDADYKVIKAGVVAT
                     DSTGYAAIQEVQQELTLDTTATTRLKKYGVNTDLYLANF"
BASE COUNT          595 a          665 c          402 g          933 t
ORIGIN      
        1 gaagtttgcc aaatataaat ctgtatttac accatatttt ttcaaacggg tagttgctgt
       61 tgtatctaat gtcaattctt gttgtacttc ctgaattgct gcatagcctg tactatcagt
      121 tgccacaaca ccggctttta taactttata atctgcatct gtcggaacag aatattttga
      181 caaaaacttc actcttccat ttgcatagga cacatcgcac aaaaccgaaa cttctttttg
      241 aatttcttct gcttcttcaa cataaacaga tgtcacagct atgtctttat atactgtaaa
      301 tgtataagat tcattgtaac agatcggttt ttcattcaca gtccagtgtg aaaacttctt
      361 tccttcagga gcctgtgctg ctacaactct tacctgatct ccgacttcac aatattttgt
      421 ttctgtgttt ccgttgttat tgatcgtaac aaccgccttt aactgcaatc cttcgatcga
      481 cgtcttcaaa gattcaacaa tcaatttcac ctcttctact gaaagagctg tattgtcttt
      541 cgctgctttt gcatctgcaa gatcgtcttc aagcgctttt ctcgttgctt ccgtatattt
      601 ctccgcaaaa ttttcttcct tcatcctgct ttctgccgca gcaatcgctt cttctaacgc
      661 cgtatcgtcg cgggtaaccg taacaataca ctgtgccgtt atattaggat aattcacaga
      721 acttacttta attgttgcat ttcccgcttt atgagctgtg acaactccgc cgtcaacagt
      781 agcgacttcc tcattatcag attcccataa aacttcttga tctgtcgcgt cttccggggt
      841 gatcgctgcc tgcagcgtcg ccgtcttctg ctcttccgtt cctccgactt tcaaagccaa
      901 tgcatcctga tctaatgtta ttcccgtaac tgcgatttct acagtttcat ataattttgc
      961 atttgcccaa cacgtatgca gtttcccgcc atttccgttg ttccatgcgc ttgcagggca
     1021 ctctagcttc atatgagtcg ttccttccgg taatttcaca cgaatacttc gcaatccacc
     1081 gtttgccgaa agatcttctg cataatactg ctgtgtataa ttacttccgt ctgtacttgt
     1141 agaaacttta aatttcgctt tcacatttgc aaaaacaccg ccgtctgacg caatctgatg
     1201 attctcgaca ccgatgtcgg cagaaaacat cttgtagcct gtcacatcat atacaatcga
     1261 agacggctca tgtgcaccta tgccttttgt atatgtattt attgtagacg tatctttctt
     1321 gattgctatc aacctgtttc catccggaca cagatctttc ttaactccac cccatgcagt
     1381 atttccggac aagtgcgtta aatccgacac ataaactcct gttcccaatt catcctgaaa
     1441 ttccattttg aactgttcca actgttggaa aacagcctca agttcttctt ttgtcgtgct
     1501 ttcctgcgct aactttgctt ctgcctctgc cttaatcgga tcaaattttg ctctggaata
     1561 tactttaccc gaatctgcgt ttccgctaag cacgttcaac tccatctgtg attccatctg
     1621 tgcaagtttt tctcgtattc tgatttttaa agcttctgtt tccgcatctt tttttaccgg
     1681 agtcaatata ctgatttctg ccgccgatat atgctgattg ggagcgcttc catccgtatg
     1741 aatgacagta agttttactt ttttgacatt ctcaacttct gcatcaaata caaattcctt
     1801 ttcggattta ttctctgccg accaattagc tcccgggttg ttataactaa tatgtaagtt
     1861 atttcctaca ttttcccaat tttcagttcc gtctttctga accgaaacac taaattctgt
     1921 cactgttccg tttccaaacg cgtcctgcct cggaagataa gtaatcccct ggatgctctg
     1981 cgcgttctga agttctactg ttatcgtatt gttgcctgtc agagcgccgt cttccctgtt
     2041 cggctgatta tctccgctat aacacgaatg ataataagac gtaatcactc cgtctgtcgc
     2101 attgactgca tgtcccgttc cgggcgatgc cgcgccttca cctgtacttg attcattatc
     2161 cgcctccgta tccgctgttg tcgtcagctc tgtataaggc actttcttat accccgattc
     2221 actgacttct tcattccata cccaatcagc gcttgcctgt acgttcaccg gaaatgcgct
     2281 tcctgtaata accatcgctc cagcgcataa taaagcgaat gtctgtttca ttttcataat
     2341 cttatccacc tcttcctttt ttctatactt gcaatccata attcaacggt ctgcagaaat
     2401 attcatatat tgcttttctt ccctcacctc ccggttccct tataattatc gaaatctctg
     2461 tttcgactcg aatgataaat gatgttatat ctttataatt ttaccccccc ccctgtattt
     2521 ttctgtcaat atgttttgtg tatttttttc taatatttct tttatatctt aatttttttg
     2581 tgcattttta ctcta
//
LOCUS       DBMDXF010000249         2572 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8726_length_2572_cov_1.371510, whole genome shotgun sequence.
ACCESSION   DBMDXF010000249 DBMDXF010000000
VERSION     DBMDXF010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8726_length_2572_cov_1.371510"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2572
                     /locus_tag="ACZPD4_08255"
     CDS             <1..>2572
                     /locus_tag="ACZPD4_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015668 - type III site-specific
                     deoxyribonuclease activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR1379082.1"
                     /translation="AAKAVADVFDGQPYLAPSYMTGEISGKNSSSEERKGTFSGWSNQ
                     KIVPELSDERILDNLRKIQKANQIPVSSKLEGRENGYHLTVEMETGVGKTYTYIKTIY
                     ELNKRYGWSKFLVVVPSIAIREGVYKTFQITREHFAKEYGKKIRFFIYNSERLTEIDR
                     FASDNSVHVMIINSQAFNAKGKDARRIYMNLDEFQGRKPIDLIAGTNPIIIIDEPQSV
                     EGRQTKERMKQFCPLITLRYSATHKADSLYNMVYRLSAADAYRKYLVKKIEVKGIAET
                     KTMASDGYIYVERICCSESDAAAVIQYDFKMGSGIRKQYRKVGIGDDLYEISGGLEEY
                     QGGFEVKQINRQEESVEFVNGMKLYAGDINGKVDEEQIRRIQIRETILSHIDRERRLF
                     GRRIKVLSLFFIDEVARYKKYDETGCPQNGSYADIFEEEYRNIIENMKYGPGDEKYRD
                     YIEMIPVEKTHAGYFSIDRKGHVVDSKGKGKEMMSDDQDAYDLIMKNKELLLECDPKQ
                     SPVRFIFSHSALREGWDNPNVFQICTLKNGGSEVRKRQEVGRGLRLCVNGDGQRMDRS
                     FLGQDVHRVNTLTVIASESYESFVKGLQSEIAEDVFGKQSRKADVCRDIEVIIPENAR
                     SRRVELKVDQEKLNGEEFSALWSNICLKSTYTVNFDTEKLVEDAVKILNRELHIQDMR
                     FKGKNEAAKSVVKYDLAGKLADMTGLKRKTVIEILSRVESSVFQQFQESPEEFIAQAE
                     RLILNVKETAISENIIYHTSDEKYTKDIFTQQTIQGKQGVDAMKVGKHLYDYVKYRSG
                     AEKEFIERLEASKEVAVYVKLPEQFYISTPAGRYMPEWAIALYRETKGKRFFVVGKKA
                     G"
BASE COUNT          889 a          394 c          670 g          619 t
ORIGIN      
        1 tgcggcgaag gcagttgcgg atgtctttga cggacagcca tatttggcgc cttcttatat
       61 gacaggggaa atatcgggga aaaattcgtc gtcagaagag agaaaaggaa ctttttcagg
      121 atggagcaat cagaagatcg tgcccgaatt aagcgatgag agaatattag ataatctgcg
      181 gaaaatacaa aaggcgaatc agattccggt ttcttcaaag ctggaagggc gggaaaacgg
      241 atatcatctg acggttgaga tggagaccgg agttgggaaa acgtacactt atattaagac
      301 gatatacgaa ttaaataaac gatacggatg gagtaagttt ctcgtcgtcg tgccaagcat
      361 cgcgattcgg gagggcgtat ataagacatt tcagataacg agagaacatt ttgcaaaaga
      421 gtatggaaag aagatccgtt tttttattta taattcagag agacttacgg aaatcgaccg
      481 gtttgcatcg gataattctg ttcatgtaat gatcatcaat tctcaggcat tcaatgcaaa
      541 aggaaaggat gcaaggagaa tttatatgaa tcttgatgag tttcagggaa ggaagccgat
      601 cgatttgatc gcgggaacaa atccgatcat aattattgat gaaccacagt cggtggaagg
      661 caggcagaca aaagagagga tgaaacagtt ttgccctctt attacgcttc ggtattctgc
      721 aacgcataaa gctgacagtc tgtataatat ggtgtatcgc cttagtgcgg cagatgctta
      781 cagaaaatac cttgtgaaaa aaattgaagt aaaaggaatc gcagagacaa aaacaatggc
      841 gtcagacggc tatatatatg tagaaaggat ctgttgttct gagtctgatg cggctgccgt
      901 tatacagtat gattttaaaa tgggaagcgg gattcggaaa caatatcgaa aggtaggaat
      961 cggcgacgac ttatacgaaa tttcgggagg tttggaagaa tatcaaggcg gctttgaagt
     1021 aaaacagatt aacagacagg aagaatcggt tgagtttgtc aacgggatga aactatatgc
     1081 aggcgatatc aacggaaaag tggacgagga acagattcgc agaatacaga tcagagagac
     1141 aatcctgtct catatagaca gggaacgccg gttgtttggc agaagaatta aagttttgtc
     1201 attgtttttt attgatgaag ttgcccgtta taaaaagtat gacgaaacgg gatgtcctca
     1261 aaacggcagt tatgcggaca tatttgaaga agagtaccgt aacattatag agaatatgaa
     1321 gtatggaccg ggagatgaaa agtatcggga ttacatagaa atgatccctg tcgaaaaaac
     1381 tcatgccgga tatttttcaa tagacagaaa agggcatgta gtcgacagta aagggaaagg
     1441 aaaagaaatg atgtcagacg atcaggatgc ttatgatctg atcatgaaaa ataaagaact
     1501 gcttttggag tgtgacccga aacagtctcc tgttcgattc attttttctc attcagcact
     1561 tcgggaagga tgggataatc cgaatgtatt tcagatttgt acgttaaaaa acggaggaag
     1621 cgaagtacga aaacggcagg aagtcggaag agggcttcgt ctgtgtgtca atggagacgg
     1681 acagagaatg gacagaagtt ttctggggca ggacgttcac agagtgaata cgttgaccgt
     1741 gatcgcaagc gaaagctatg aaagttttgt gaagggcctg caaagtgaaa ttgcagaaga
     1801 tgttttcggt aaacagagtc ggaaggccga cgtctgtcgc gatatagaag taatcatacc
     1861 cgagaatgca agaagccgtc gagtagaact aaaagtcgat caggaaaagc taaatggaga
     1921 agaattcagc gcattgtggt caaatatttg cctgaaatcg acatataccg taaattttga
     1981 tacggaaaaa ttagtagaag atgcggtgaa aatattaaac agagaactgc atatacaaga
     2041 tatgcgattt aaaggcaaga atgaggcggc aaaaagcgtc gtgaaatatg atcttgcggg
     2101 gaaacttgcc gatatgacag ggctgaaaag gaaaacagtg atcgaaatat tgagcagagt
     2161 cgaaagttct gtttttcaac agtttcaaga aagtccggaa gaatttatcg ctcaggcaga
     2221 aagattgatc ttaaatgtga aagaaacagc gatttcggaa aatatcatat atcatacgtc
     2281 agacgaaaaa tatacaaaag atatttttac acagcagacg attcagggaa aacagggagt
     2341 ggacgcaatg aaagtcggga agcatttgta tgattatgtg aaataccgat ccggcgcaga
     2401 aaaggaattt atcgaacggc tggaagcatc aaaagaggtt gcggtatatg taaaacttcc
     2461 ggaacaattt tatatctcca ctcctgcagg acgttatatg ccggaatggg cgatcgcttt
     2521 gtaccgggaa acgaaaggaa aacgtttctt tgttgttggg aaaaaagccg ga
//
LOCUS       DBMDXF010000250         2568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8746_length_2568_cov_1.699230, whole genome shotgun sequence.
ACCESSION   DBMDXF010000250 DBMDXF010000000
VERSION     DBMDXF010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8746_length_2568_cov_1.699230"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="ACZPD4_08260"
     CDS             complement(<1..2094)
                     /locus_tag="ACZPD4_08260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1379083.1"
                     /translation="MAVYDTLNKPQKEAVFHTEGPLLILAGAGSGKTRVLTHRIAYLI
                     EEKGVNPWNILAITFTNKAAEEMRQRVDSLVGIGAESIWVSTFHSMCVRILRRYIDRL
                     GYDNRFTIYDTDDQKTLMKEVCRKTDIDTKRFKERMLLSVISSAKNEMILPEEFELNA
                     GGDFVQLKIAKVYKEYEAQMRANNALDFDDLLVKTVQLLETQPDVRENYQERFRYIMV
                     DEYQDTNTVQFRLVSLLAGKYRNLCVVGDDDQSIYKFRGANIRNILDFEKEFSDAKVI
                     KLEQNYRSVGNVLEVANSVIRNNKGRKEKTLWTDNEKGEKIRLRQFDTAYDEAQFIAE
                     DIKDETAQGANYSDHAVLYRTNAQSRLLEEKFVAMNIPYKIVGGINFYSRREIKDVLS
                     YLKTIDNGKDDLAVRRIINVPKRGIGLTTINRIQESAAARGIGFYDALSAPDLIPGIG
                     RSASKLDSFAALIEYFKGRSEESGVTDLLTEVIEKTGYTESLEADDPEELEARVQNID
                     ELVSKAAVYEESCSDRGERPTLSGFLEEVALVADIDSVAEDRDYVILMTLHSAKGLEF
                     PHVYLAGMEDGLFPSYMSISGDDPEELEEERRLCYVGVTRAEEKLTLTCARMRLVRGE
                     RQYNSMSRFIKEMPSALIDTGKREGGFSQNVSLGEKRTYSYEVSGYKRSAYAQKPAFA
                     AIQKGSGLMAKKSEGL"
     gene            2397..>2568
                     /locus_tag="ACZPD4_08265"
     CDS             2397..>2568
                     /locus_tag="ACZPD4_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR1379084.1"
                     /translation="MKIDMTDLLNSITENLKRIDYIKPEEIPNIDLYMDQVTTFMEEQ
                     LVSSKRYADDKIL"
BASE COUNT          689 a          677 c          414 g          788 t
ORIGIN      
        1 gagtccctcc gactttttcg ccatcagtcc gctccccttt tggatcgcgg caaatgccgg
       61 tttctgagca tacgcagatc ttttgtatcc actgacctcg tacgaatacg tcctcttctc
      121 tcccaaagaa acattttgag aaaaaccgcc ttctcgcttt cctgtatcaa tcaatgcaga
      181 aggcatctct ttaatgaaac gggacatact gttatattgt ctctcacctc tgaccaaacg
      241 catacgcgcg caggtaagag tcaatttttc ttcagcgcgg gtcacgccca cataacaaag
      301 tcttcgttcc tcctccaact cttccggatc atctccgctg atcgacatat aacttgggaa
      361 caatccgtct tccattcccg caagatatac atgtggaaat tccagacctt tcgcactgtg
      421 aagagtcata aggatcacgt aatcccgatc ttccgctacg ctgtcgatat cagcgacaag
      481 agccacctct tcaaggaaac cgctcagcgt cggtctttct cctctgtcac tgcagctttc
      541 ctcatatacg gccgccttac tgacaagttc gtctatattc tgcactcttg cttccagttc
      601 ctcaggatca tccgcttcca gactttccgt ataccctgtc ttctcgatca cttccgtcag
      661 aagatctgtc accccgctct cttccgatct tcctttaaaa tattcgatca aagctgcaaa
      721 tgaatcaagt ttcgatgcgc tgcgtccgat cccgggaata aggtccggag ctgaaagggc
      781 gtcataaaac cctattcctc tcgcagcagc cgactcctga atccgattaa tcgttgtcaa
      841 accgatcccc cgtttcggaa cattaataat ccttctcaca gccagatcgt cttttccgtt
      901 atcaatcgtt tttaagtatg acaaaacatc cttaatctct cttcgggaat aaaaattgat
      961 gcctccgaca atcttatacg gaatattcat cgcgacaaac ttttcctcaa gaagacgcga
     1021 ctgcgcgttc gttcggtata aaactgcatg atcactgtaa tttgctccct gtgccgtttc
     1081 gtccttaata tcctctgcaa taaactgcgc ttcgtcatat gccgtatcaa actgccgcag
     1141 acggatcttc tccccttttt cattatcagt ccaaagagtc ttttcttttc tgcctttatt
     1201 gttgcggatc acactattcg caacttccaa tacattcccg acagagcggt agttctgttc
     1261 cagtttaatt acttttgcat ctgaaaattc tttttcaaaa tccaaaatat ttcgtatatt
     1321 agctccgcgg aacttataga tcgactggtc gtcatcgccg actacacaca aattcctata
     1381 ctttcccgca agcaggctga caagccgaaa ctgtacggta ttcgtgtcct gatattcgtc
     1441 aaccatgata tatctgaaac gttcctgata attctccctc acatcaggct gcgtttccag
     1501 aagctgaact gtctttacaa gcaaatcgtc aaaatccaac gcattattcg cccgcatctg
     1561 cgcctcatat tctttataga cttttgcgat cttaagctgt acaaaatctc ctccggcatt
     1621 caattcaaac tcttccggaa ggatcatctc gtttttagca gaagaaatta ccgataaaag
     1681 catcctttct ttaaaccgct ttgtatcaat atctgtctta cgacatactt ctttcatcag
     1741 cgtcttctga tcatccgtat cataaatcgt aaatctgttg tcatatccga gacgatctat
     1801 gtatcttctt aagatccgta cacacatgga atgaaacgta ctgacccaaa tactctctgc
     1861 tccgatcccg acaaggctgt ctactctctg acgcatctct tccgccgctt tatttgtaaa
     1921 cgtaatggca agaatattcc atggattcac tcccttttcc tcaatcaggt atgcaattct
     1981 gtgagtcaaa actcttgttt ttcccgaacc cgctcccgcc aggatcaaaa gcggaccttc
     2041 cgtatgaaat acagcctcct tttgaggttt attcaacgta tcatatacag ccatttatat
     2101 gctccttatc tttctttttc tcaattaccc gtaaaatatt tagttcaaat aacgagtgtg
     2161 tattgttctt attatcgaac atattttcgt taaagtcaac cttctttttt cgttcattta
     2221 tcccatgcca aacgcttcca tatcgttatc atttatcaag gcgcactcct gataaaacgg
     2281 ctgataacta catttttata caaaaacaac gatgggaaat cccggttaat aaaggtttag
     2341 aaaaggcttg ccatctagtt ttcactacta tataataagg atatcgaggt gatattatga
     2401 aaatagacat gacagatctg ttaaacagca taacagaaaa cttaaaacgt attgattaca
     2461 tcaaaccgga agaaataccg aatattgatt tatatatgga tcaggtaaca acctttatgg
     2521 aagaacagct cgtttcttcc aaacgttacg cagacgacaa gattctca
//
LOCUS       DBMDXF010000251         2559 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8787_length_2559_cov_1.975183, whole genome shotgun sequence.
ACCESSION   DBMDXF010000251 DBMDXF010000000
VERSION     DBMDXF010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8787_length_2559_cov_1.975183"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(143..1558)
                     /locus_tag="ACZPD4_08270"
     CDS             complement(143..1558)
                     /locus_tag="ACZPD4_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1379085.1"
                     /translation="MKRMSPARAIAYGLPGLATLFTFTMFTTYGLYFFTNIVGLSGKF
                     AGLIMTIGTLWDAVTDPLVGIISDNRDPRKGRRRPFLLVCAIPFGIVTWLLFTAWDFV
                     EIQQKIYFIIVALLFYTFQTLIDVPYTSLSGEVTDNYDLRSKLATIRTFWAIIGVAIG
                     GGIMAYTDFLEPVVGGSRQAWSVCFAFFGIICTLSIWIGYKASEGYENKDIITKKSYS
                     IKEMLEGPLRNKPFLHLTIAFIFAILAQAIFLGVLVYYLRYNLNLNDTQVSLVNIIMW
                     IVALFWVFPIDHWSTKYSKVVSWTISMGIWLLCMIVFPLFFLKPGSTAAPIIMTSILV
                     VGLNALYQVIYAMISDCVEVDELMSGERREGLFYSMATVSQKLAAASGVSVLGMIIDY
                     VGYNPAVSVQSMQTVQGFHNIFVIGTSVCLLLSIVTMATNPLTKKRYSEVLEVVRLKR
                     AGKEIALDEFKDLLFIKKNRK"
     gene            complement(1560..>2006)
                     /locus_tag="ACZPD4_08275"
     CDS             complement(1560..>2006)
                     /locus_tag="ACZPD4_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379086.1"
                     /translation="VRRDLVHLGTLAWCFLIGPFENFWLDVLAPMLPTAPEMSVGLKI
                     ACILIGSIIIILTLAYYIPIGLGYQTSDIFAFMAADLVKKSYGIGLIISYAILFVIGV
                     ALGSSWGIGTLIAVVAYGPIIDRLMGIIEPFSFAVAGMKKENKKGK"
     assembly_gap    2007..2016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2017..2234)
                     /locus_tag="ACZPD4_08280"
     CDS             complement(<2017..2234)
                     /locus_tag="ACZPD4_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379087.1"
                     /translation="MDAKKIWSYARRLLAIIPSIFICALGVALLVWPEWGCDPLTTFE
                     IGLSNVFHVQFGTVALVFEGIMFFVFFFV"
     gene            complement(2234..>2559)
                     /locus_tag="ACZPD4_08285"
     CDS             complement(2234..>2559)
                     /locus_tag="ACZPD4_08285"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019441 - L-tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1379088.1"
                     /translation="FERYLKENNISDTWESMNLNTFEGYQKYLVDNGVRFIGIDTINA
                     DQTGGTEFPVHDLFSEKRLMIGENWAFFDQIDFERPYVIALPMKLIGCDGSPVRAIAV
                     QWEEE"
BASE COUNT          825 a          621 c          409 g          694 t
ORIGIN      
        1 ctttacaaaa tttaaacctg tatcacagtc cccacacatc tcccttaaaa tataaaaact
       61 ctctttccca cctcaacaca ggaaggtcat atgcatttcc ttccttatat aaaatgcaaa
      121 aagagagttt tcatatgtta tattattttc ggttcttttt tataaacaat aaatctttaa
      181 attcatctaa agcaatctct ttccctgccc ttttcaagcg cacgacttcc aacacttcgg
      241 aataacgttt tttcgtcaga gggtttgttg ccattgtcac aatggacagc agcagacaga
      301 cagaagttcc tattacaaat atattatgaa acccttgaac tgtctgcata ctttgcacgg
      361 ataccgccgg attatatccg acataatcta taatcatccc taaaacactg acgccggacg
      421 cagcggctaa tttctgggat acggttgcca tagaataaaa caacccttcc cgtctttcac
      481 cgctcatcaa ttcatcgact tctacgcagt cagaaatcat ggcataaata acctgataca
      541 gtgcgtttaa cccaactacg agaatgctcg tcataatgat cggcgctgcg gttgaacccg
      601 gctttaagaa gaacaaaggg aacacgatca tacacaaaag ccagattccc atagaaatcg
      661 tccatgaaac aacttttgaa tatttcgtag accaatgatc tatcgggaaa acccaaaaca
      721 acgctacgat ccacataatg atattcacaa gggatacctg cgtgtcgttc aggttcaggt
      781 tatatctcag ataatatacc aatactccga gaaaaatcgc ctgtgccaaa attgcaaata
      841 taaacgcaat cgttaaatga agaaacggct tgttccgaag aggaccttcc agcatttctt
      901 ttatggaata agattttttt gtaataatat ctttattttc gtatccttca ctcgccttat
      961 aaccgatcca gatactaagc gtacagataa tgccgaaaaa tgcaaaacat acggaccaag
     1021 cttgtctgct gccgccgaca acaggctcta aaaaatcggt atatgccatg atcccgccgc
     1081 cgattgccac tccgatgatc gcccaaaacg ttcttattgt agccagctta cttcgcaggt
     1141 catagttatc ggtaacttcg ccgcttaagg aagtatacgg aacgtcgatc aaagtctgaa
     1201 atgtataaaa taacaaagca actataataa aataaatttt ctgctgaatt tcaacaaaat
     1261 cccatgccgt aaacagtagc cacgttacaa ttccaaacgg aatcgcacat actaataaaa
     1321 acggtcttct tctcccttta cggggatcgc ggttatctga gatgattccg accaaaggat
     1381 ctgtaacagc atcccataaa gttccgatcg tcatgatcag acccgcgaat tttcctgaca
     1441 atccgacaat atttgtaaaa aaatacaagc catatgtcgt aaacatggta aatgtaaaaa
     1501 gcgttgccaa gccgggcagt ccatatgcta tcgcccttgc aggtgacatc cttttcatct
     1561 tacttgcctt tcttattttc ttttttcatt cctgccacag caaatgagaa cggctcaata
     1621 atccccatca gcctgtctat gatcggtccg tatgcaacaa ctgcgatcaa tgttccgatt
     1681 ccccatgacg atcctaacgc cactccgatt acaaataaga ttgcataaga tatgatcaga
     1741 ccgattccgt aactcttttt aacaagatca gccgccataa acgcaaatat atcggatgtc
     1801 tgatatccta aaccgatcgg gatataataa gccaatgtca aaataataat gatcgaacct
     1861 attaaaatac acgctatttt cagtccgaca ctcatttccg gagctgtcgg cagcatcggt
     1921 gcaagaacgt ctaaccagaa attttcaaaa ggtcctatta aaaagcacca tgcaagagtt
     1981 cctaaatgta ccaaatctct tcttacnnnn nnnnnnacaa agaaaaatac aaaaaacatg
     2041 attccttcaa acacaagagc aacagtacca aactgaacgt gaaatacatt tgataaccca
     2101 atctcaaatg ttgtcagcgg atcacatccc cattccggcc aaaccaaaag cgctactcca
     2161 agagcacaga tgaaaataga agggataatt gcgagcagcc ttctggcata actccagatt
     2221 tttttcgcat ccattattct tcctcccact gtaccgcgat cgctcttaca ggcgaaccgt
     2281 cgcatcctat aagtttcatc ggcagtgcga taacatacgg acgttcaaaa tcgatctgat
     2341 caaaaaatgc ccagttttct ccgatcatca atcttttttc agagaacaga tcgtgtaccg
     2401 gaaattccgt tccaccagtc tgatccgcat tgatcgtatc aattccgata aagcgaaccc
     2461 cattatcaac taaatatttt tgatagccct caaaggtgtt taaattcatg gattcccaag
     2521 tgtcagagat attattttct tttagatacc tctcaaatt
//
LOCUS       DBMDXF010000252         2543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8852_length_2543_cov_1.813677, whole genome shotgun sequence.
ACCESSION   DBMDXF010000252 DBMDXF010000000
VERSION     DBMDXF010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8852_length_2543_cov_1.813677"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            66..1097
                     /locus_tag="ACZPD4_08290"
     CDS             66..1097
                     /locus_tag="ACZPD4_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1379089.1"
                     /translation="MKKKKLLAAAIVLCMVIQLLCACGKQKISMSDKVYVGVTCYDQN
                     DTFLSELLVCFKEELNHLNKNGVETTVTVKNAAGSQRTQNDQVKELINAGCNVLCVNL
                     VDRADPSEIIDVAKEHDVPIIFFNREPVAEDMRQWDRLYYVGADAKQSGVLQGELAVE
                     MIRQNSQIDHNKDGKIQYVVLEGEAGHQDAIIRTENAVDTLNKNGIELEKLSYQIANW
                     NRAQAQNRMEQMLGQYKNKIELVLANNDDMALGALDAYKNLNYTEALLPVFIGIDGTD
                     MGLKAVEDSSMAGTVYNDKEGQARAIAKLSAALVIGKEAEEIKFTNENYIYLPYSKVT
                     KENVHEYLK"
     gene            complement(1212..2384)
                     /locus_tag="ACZPD4_08295"
     CDS             complement(1212..2384)
                     /locus_tag="ACZPD4_08295"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1379090.1"
                     /translation="MKQKLYDKFAELFGATDDAHFYFAPGRVNLIGEHTDYNGGHVFP
                     CALTLGTYGVARKREDRLMHFYSCNLDEIGVVETSLDDLTNKDCYDWANYPLGVVWTF
                     SEKGYKLDTGFDMVIWGNIPNGAGLSSSASLEVLTGVILTDLYGITDLSPIDLALFGQ
                     YSENNFNGCNCGIMDQFTVAVGKKDNAIFLDTNTLQYEYAPILLEDSKIIITNSKVKH
                     SLVTSEYNVRRQECADALSDLQKELSISSLGELTPEEFEEAKHLIRDEVRVKRAKHAV
                     YENQRTIAAVKALKEGDITKFGELMNQSHVSLRDDYEVSCKEIDLLVDLSWNTPGVIG
                     SRITGGGFGGCTVSIVKNEAVDNFIKNVGEAYKEKFGYEAEFYVVDIGVGASRLDV"
BASE COUNT          782 a          464 c          598 g          699 t
ORIGIN      
        1 agttttgttc actatgagtc agtaaggcgt atagagtaca tgaggattat aacagaggtg
       61 gaggaatgaa aaaaaagaaa ctattagcgg cagcgattgt tttatgtatg gtgatacaac
      121 tcttgtgtgc ctgcggtaag cagaaaatat ccatgtcaga caaggtttat gtcggagtta
      181 cctgttacga tcaaaacgat acctttttaa gcgaacttct tgtctgtttt aaagaagaac
      241 ttaatcatct caacaagaat ggagttgaga cgactgtgac agttaaaaat gctgccggat
      301 ctcagcgcac gcagaatgac caggtgaaag agctgataaa tgcaggatgc aacgtccttt
      361 gtgtgaatct ggtagatcgg gcagatccgt cggaaattat agatgttgca aaagaacatg
      421 atgtgccgat tatatttttt aacagagaac cggttgcgga agatatgagg cagtgggaca
      481 ggctttatta cgttggcgca gatgcaaagc agtccggtgt gctgcaagga gagctggctg
      541 ttgaaatgat acggcaaaac agtcagatag atcacaataa ggacggaaaa attcagtatg
      601 tagttctgga aggggaagcg ggacatcagg atgcgatcat tcgtacagaa aatgcggtag
      661 atacgcttaa caaaaatggg attgaactgg aaaagttaag ttaccagatt gcgaactgga
      721 accgggcgca ggcgcagaac cggatggagc agatgctggg gcagtataaa aataagatag
      781 aacttgtgtt ggcaaataat gatgacatgg ctctgggagc gttggacgct tataaaaatt
      841 tgaattatac agaggccctt cttccggtat ttataggaat agacggcacg gatatggggt
      901 tgaaggcagt ggaagattca agtatggcgg gaacggtata taatgataaa gagggacagg
      961 cgagagcgat agcaaagctt tcagctgcat tggtaatcgg aaaagaagcg gaggaaataa
     1021 aatttactaa tgaaaattac atttatctgc cctattccaa agtcacaaaa gaaaatgtcc
     1081 atgaatatct gaagtgacgg atgatccgct gagcaaggtg aaaggcagaa aaaaacacac
     1141 cgggtaaaag agttttggta tcttgtacaa ggtgtgtttt ttgtgttttt aaaagtttgt
     1201 tttacggaag attatacatc taatctggaa gcgccgacac cgatgtctac tacataaaat
     1261 tcagcttcat atccgaattt ttctttatag gcctctccga catttttaat aaaattgtct
     1321 acggcttcat tttttacaat gctgacggta cagccgccga atccgcctcc ggtgatacga
     1381 gagccgatga ctcccggggt attccatgaa agatcaacga gcagatcgat ttctttacat
     1441 gatacttcat aatcatcgcg cagagaaaca tgggattgat tcattaattc tccgaacttt
     1501 gtaatatcgc cttctttcag agctttgacg gcggcgatag tgcgctgatt ctcgtaaaca
     1561 gcatgtttgg cacgttttac acgaacttca tcccggatca ggtgctttgc ctcttcaaat
     1621 tcttctggag taagctcgcc gagagaagaa atggacagtt ccttttgcag atcgctaagc
     1681 gcatccgcac attcctgacg tcggacattg tattcagaag tgacaaggct gtgctttact
     1741 ttactgtttg taatgatgat cttggagtcc tcaagaagaa tgggtgcata ttcatattgc
     1801 aaagtgtttg tgtcaagaaa tattgcgttg tcttttttcc cgacagctac tgtaaactga
     1861 tccataattc cacagttaca gccgttaaaa ttattttcgg agtattgtcc gaataaagca
     1921 agatctattg gacttagatc ggtaattcca tataaatcag tcaggatcac tccggtcagc
     1981 acttccagag aagcagaaga ggaaagacct gcaccgttag gaatattccc ccatattacc
     2041 atgtcaaatc cggtatcaag tttgtagcct ttttcggaga atgtccatac aactccgagc
     2101 ggatagtttg cccagtcgta acaatccttg ttagtaaggt cgtccagaga tgtttctact
     2161 acgccgatct cgtcaaggtt gcaggaataa aagtgcataa gccggtcttc tctttttctt
     2221 gcaacgccat atgtaccaag tgtcagggca caggggaata catgaccgcc gttatagtcg
     2281 gtgtgctctc cgataaggtt gacgcggccg ggagcaaaat aaaaatgtgc atcatccgta
     2341 gcgccgaaaa gttctgcaaa tttgtcatat agtttttgtt tcatacgtgg tttcctccta
     2401 cataaaatat tgaaataata aattcaaaac atcttgcctt ttattataat ctatagcgga
     2461 agaaatgtat agaaaaaatg agcgcgaata taggtaaaaa tgttgtattt ttagctttta
     2521 taagacaaaa gtatcccaac acc
//
LOCUS       DBMDXF010000253         2540 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8862_length_2540_cov_1.963100, whole genome shotgun sequence.
ACCESSION   DBMDXF010000253 DBMDXF010000000
VERSION     DBMDXF010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8862_length_2540_cov_1.963100"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(9..152)
                     /locus_tag="ACZPD4_08300"
     CDS             complement(9..152)
                     /locus_tag="ACZPD4_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379091.1"
                     /translation="MGENYLDMVPVIVIFFALGILLSRLGGVGGIVVRIKRMICKLIG
                     HRD"
     gene            complement(468..1121)
                     /locus_tag="ACZPD4_08305"
     CDS             complement(468..1121)
                     /locus_tag="ACZPD4_08305"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR1379092.1"
                     /translation="MSKQTVIVSGGMVEKDFALSILKSEETEFVIGVDRGLVFLYDNE
                     IKPDYIVGDFDSVPRKLVEYYREELDVPVREFNPVKDASDTEIALRLCLGLNRKKIVI
                     LGATGNRIDHLWANVQCLQIALEAGADACIMDAHNKIRLLDKTVTLKKEEAFGPYFSL
                     FPLEQPVDAFNITGAKYPLTNHFLKPSDSLCVSNEFVEDEAEISFVYGKVILMETRD"
     gene            complement(1114..1776)
                     /gene="rpe"
                     /locus_tag="ACZPD4_08310"
     CDS             complement(1114..1776)
                     /gene="rpe"
                     /locus_tag="ACZPD4_08310"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1379093.1"
                     /translation="MENILAPSILSADFKILGEQIEQTEKAGAKYIHFDVMDGIFVPS
                     ISFGMPVLSALKGAVKQALDVHLMVTEPIRYVKEFAKCGADIITVHLEACEDVKATVD
                     EIKASGVKAGITIKPATPVKELIPYLELADMFLIMSVEPGFGGQAFIPESLDKIKELR
                     TLLDERGLKTDIEVDGGIYCENVEAVLDAGANIIVSGSGVFKGNITENTKRFMEILKR
                     HE"
     gene            complement(1779..>2540)
                     /gene="rsgA"
                     /locus_tag="ACZPD4_08315"
     CDS             complement(1779..>2540)
                     /gene="rsgA"
                     /locus_tag="ACZPD4_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1379094.1"
                     /translation="EVLDEEEKKGNIREILTRKNELIRPAVANIDQALVVFAVTKPKP
                     HFNLLDRFLVMMEQKKIPVILCFNKSDIAKESDISKMEEIYRSCGYPVFFTSAKEGWK
                     IEEVKKILHGKTTVLAGPSGVGKSSLINLLQSEVMMETGSISRKIDRGKHTTRHSELL
                     VLEEDEKVEDCGSYIVDTPGFSSLYVNDFEKEQLKYYFPEFGPYEGLCRFSGCDHVHE
                     PDCAVKQAAEEGKIHEIRYNDYVAMYRELQEKRRY"
BASE COUNT          683 a          660 c          401 g          796 t
ORIGIN      
        1 aatttctctc aatctctatg tcctattagc ttacaaatca ttcttttaat tcttacaaca
       61 attcctccaa ctcctcccag ccttgataat aaaatgccga gagcaaaaaa tatcactata
      121 acaggaacca tgtccaaata attttctccc atagtcgatc tcctttacag attctaatgt
      181 gttttttcag tatactataa ttataatcat tctatcagac aaaagctttt atttctacgg
      241 aattttctgc aaagcatata tctacgtatt gttaaactgt atcatagaat ataactaaaa
      301 ggtaacctgc ctcagcaagt tacctttttt caacgcctta caaaaaattt tctcttttct
      361 cataaaatcc tgatatgctt tctcatttac tataccggga ctttgagaaa aacatccggg
      421 aattagtggg gcacaaagtg aaccatcttt caaaaactta tcgaaatcta atctctcgtc
      481 tccatcaaga tcaccttacc gtacacgaac gatatttccg cttcatcttc aacaaattca
      541 ttacttacgc acaggctgtc gctcggctta aggaaatgat ttgtcagcgg atattttgcc
      601 cctgtaatgt tgaacgcatc tacaggctgt tctaatggaa acaatgaaaa gtacggtccg
      661 aatgcctctt cttttttcaa tgtaacagtt ttatcgagaa gccgtatctt attgtgagcg
      721 tccataatgc aagcgtccgc tcctgcttcc agcgcaattt gtaaacattg cacatttgcc
      781 cacaggtgat cgatccggtt gccggttgcc cccaggatga cgatcttttt gcgattcaga
      841 ccaaggcaaa ggcgcaaagc gatctctgtg tctgatgcat cttttaccgg attaaattct
      901 ctcacgggca catcaagttc ttctctgtaa tattcaacga gcttcctcgg aacgctgtca
      961 aaatctccca cgatataatc gggttttatc tcgttgtcat acaaaaacac aagacctctg
     1021 tctaccccga taacaaattc agtttcttcg ctttttaaaa tcgagagagc aaaatctttt
     1081 tccaccatcc cgccgctcac gatcacagtc tgcttactca tgacgtttca atatctccat
     1141 aaatctcttt gtattttctg taatattgcc tttgaaaact cccgaaccgg aaacaataat
     1201 attcgctccc gcatccaata ccgcttccac attttcacaa taaattccgc cgtcaacttc
     1261 gatatccgtc ttaagacctc tctcgtcaag cagggtgcga agttctttta tcttgtcaag
     1321 cgattccgga atgaacgcct gtccgccgaa tcccggctca acgctcatga tgagaaacat
     1381 atccgcaagc tctagatacg ggatcaattc tttcacagga gttgccggtt tgatcgtgat
     1441 gcctgctttc actccgcttg cctttatttc atccactgtc gcctttacat cttcacacgc
     1501 ctctaagtga acagtaatga tatccgctcc gcattttgcg aactctttca catacctgat
     1561 cggctcagtc accataagat gtacgtcaag cgcctgtttt accgcgcctt ttaacgcgga
     1621 caataccggc atgccaaacg aaatgctcgg tacgaaaatt ccgtccatca catcaaaatg
     1681 tatatatttt gcgcctgctt tttctgtctg ttcgatctgt tctcccagaa ttttaaaatc
     1741 agccgaaaga atcgacgggg caagaatatt ttccatcttc aatatctcct tttttcctgt
     1801 aattcacggt acatcgcaac ataatcgttg taccggatct catgtatctt tccttcttct
     1861 gccgcctgct tcaccgcaca gtccggctca tgcacatgat cgcaccccga aaaacggcac
     1921 aatccttcat acggaccgaa ttccgggaaa taatatttta attgttcttt ttcaaaatca
     1981 ttgacataaa gggaactaaa tcccggagta tctacaatat atgagccgca gtcttccacc
     2041 ttttcatctt cttccaaaac taaaagttcg gaatgtctcg tcgtatgctt tcctctgtcg
     2101 atcttcctgc tgatactccc tgtttccatc attacttccg actgcagaag attgataagc
     2161 gacgacttcc ctacacccga cgggcctgca agaacagtcg ttttcccgtg cagaatcttt
     2221 ttcacttctt cgatcttcca tccttctttc gcactcgtaa aaaagaccgg atacccgcat
     2281 gatctgtata tttcttccat cttggaaata tcgctttctt ttgcaatgtc agatttgtta
     2341 aaacataaga ttaccgggat cttcttttgc tccatcatca caagaaaacg atcaagaaga
     2401 ttaaaatgag gtttaggctt cgtcaccgca aatacaacga gcgcctgatc gatattcgcc
     2461 accgccggac ggatcagttc gttctttctc gtaaggattt ccctgatatt tccctttttt
     2521 tcctcttcat ctaaaacttc
//
LOCUS       DBMDXF010000254         2520 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8943_length_2520_cov_2.052088, whole genome shotgun sequence.
ACCESSION   DBMDXF010000254 DBMDXF010000000
VERSION     DBMDXF010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8943_length_2520_cov_2.052088"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="ACZPD4_08320"
     CDS             complement(<1..119)
                     /locus_tag="ACZPD4_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1379095.1"
                     /translation="MPKPRTSTGEKNLISKRLIKLREKHNMSQRLLAYQLQLNG"
     gene            355..1176
                     /locus_tag="ACZPD4_08325"
     CDS             355..1176
                     /locus_tag="ACZPD4_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1379096.1"
                     /translation="MPTTYAHYKFGKEVISALPRPLRSTVENHRELFDIGLHGPDILF
                     YYHPMKRNTVNGQGYDLHDKPADLFFRHAAETVKKAEDPAAARAYIYGVICHFALDSE
                     CHPYVEKMIHESGISHSEIEMEFDRLLLKEDYINPVRYLATKHIRPTKENGAVIAPFF
                     EDLTAETVQKALKGMIMYHKLLLAPGAVKRKILFGGMRLSGQYDSLHGMVMSLEPNPA
                     CKEYCRLLKRLFAGAVPLAAGLIIRYQKVLFEGDEIPERFTRTFGAGEGWKDLPL"
     gene            1298..>2520
                     /locus_tag="ACZPD4_08330"
     CDS             1298..>2520
                     /locus_tag="ACZPD4_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1379097.1"
                     /translation="MKFKLTSLEKKWVLYDVGNSAFTMMVSTIFPIYFNYLAGNAGIS
                     DVDYLAYWGYATSVCTLLVAVLGPTLGAIADTKNFKKTVFFVAMGVGVFGCILLGFLS
                     SWIWFLGIFVLAKTGYSASLIFYDAMLTDVTDPERMDAVSSHGYAWGYIGSCIPFVAC
                     LGVVLGAKPLGIGMQTAMIIAFIITALWWLLSSVPLLRSYRQKYFAEVNGHVVKDSFK
                     RLGKTFAELVKEKHIFVFLLAFFFYIDGVYTVIDMATAYGQALGLDSTGLLLALLVTQ
                     IVAFPSVLIFGRIVKKVSPEKIITVCIMAYFGIAVYAYWLDTQFDFWMLAVLVGMFQG
                     TIQALSRSYFAKIIPAEKSGEYFGIYDICGKGASVIGTALVSFISQMTGSINIAVSAL
                     SVMFLVGLVLFRYSA"
BASE COUNT          649 a          438 c          673 g          760 t
ORIGIN      
        1 ccgtttaatt gaagctgata agctaaaagc cgctgcgaca tattatgctt ctctcgaagt
       61 ttgatgagtc ttttactgat cagatttttt tctccggtag atgttcttgg tttcggcata
      121 agtatattcc tttctgagaa cggtgcgctt gaaaatgtct cattttctgc actttttatt
      181 gattttacaa gggaaaagat ctgcgcgggg ttgtaaaagt gatacaaaat atcggatatc
      241 gaacgagaag tttgcaaaaa tttaacatat caaacggagt gtaattctgg attttttgtc
      301 ttgaatatgt gataataaaa ttaaaagaaa aagtacgtga aaagaggaag atttatgcct
      361 acgacatatg cacattacaa atttggaaaa gaagtgatca gcgcgcttcc gaggccgctt
      421 cgcagcacag ttgagaatca cagagaatta tttgatatcg gacttcatgg tcctgatatt
      481 cttttttatt atcatccgat gaagagaaat acagtgaacg gtcagggata tgaccttcat
      541 gataagccgg cggatctgtt tttccgccac gctgcggaaa ctgtgaaaaa agcggaagat
      601 ccggcagcgg caagagcata tatttacggt gtcatctgtc attttgcttt agacagtgag
      661 tgccatccgt atgttgagaa gatgatccat gaaagcggaa tcagccattc ggagatcgag
      721 atggagtttg accggcttct cttaaaggaa gattatatta atcctgtgcg ttatcttgcc
      781 acaaagcata tccgtccgac gaaggagaac ggggcggtca tagcgccgtt ttttgaagat
      841 ctgacagcag agacggtaca aaaagctttg aaagggatga ttatgtatca taagcttttg
      901 ctggcgcccg gcgcggtaaa aaggaagatc cttttcggcg gaatgcggct atcagggcag
      961 tatgattctc tgcatgggat ggttatgagt cttgaaccga atccggcgtg taaagagtac
     1021 tgccgtcttt taaaaaggct gtttgccggg gcagttccgc tggcggcggg actgatcatc
     1081 cgttaccaga aagttttgtt tgagggagac gaaattccgg aacgttttac aagaacgttc
     1141 ggcgcaggtg agggatggaa agatctgcct ttataaacag gcgataagct aaagcaaaac
     1201 atgagccgaa gaccgggcag atgtggaaag gatgcggtac agccgtcatg cggttcgggg
     1261 ctggcgatgg cagaatgagg gaaagggaga caaggcgatg aaatttaaac tgacttcttt
     1321 ggaaaaaaag tgggttcttt atgatgtggg aaattcggcg tttacaatga tggtatcgac
     1381 catatttccg atttatttta attatcttgc gggaaacgcc ggtatatcgg acgtagatta
     1441 tctggcgtac tggggatatg caacttctgt gtgcacgctt cttgtggcgg ttttagggcc
     1501 gacgctcgga gcgattgcgg atacgaaaaa ttttaagaaa acagtgtttt ttgttgcgat
     1561 gggagtaggt gttttcggat gtattttgtt agggtttctg tcatcgtgga tttggttttt
     1621 aggtattttt gtactggcaa agaccggata ttcggcaagt ctgatttttt atgacgcgat
     1681 gcttactgat gtgaccgacc cggaacggat ggacgcagtt tcgtctcacg gctatgcgtg
     1741 gggatatatc ggaagctgta ttccgtttgt ggcatgtctc ggcgttgtgc tcggagcaaa
     1801 accgttgggg atcggaatgc agactgcgat gatcattgcg tttattatta ctgcgctttg
     1861 gtggctgtta tcgtctgtac cgcttcttcg ctcttacaga cagaaatatt ttgccgaagt
     1921 aaacggacat gttgtaaaag acagttttaa gcggcttggg aaaacttttg cggagttggt
     1981 taaggaaaag catatttttg tatttttact tgcgtttttc ttttatattg acggtgtgta
     2041 taccgttatt gatatggcga cagcgtacgg acaggctctt ggattggaca gcacgggact
     2101 tttgctcgcc cttcttgtga cacagatcgt tgcgtttccg tctgtactta ttttcggaag
     2161 gatcgtaaag aaagtttctc cggagaagat cattactgtc tgtatcatgg catattttgg
     2221 aattgcagtg tatgcatatt ggctggatac tcagtttgat ttctggatgc tcgcagttct
     2281 tgtgggaatg ttccagggaa cgatacaggc gctttcaaga tcatattttg cgaagatcat
     2341 accggcggag aagtcgggag aatatttcgg catttatgac atttgcggca aaggcgcatc
     2401 ggttatcgga acggcgctcg tatcttttat cagtcagatg acggggagca tcaatattgc
     2461 tgtcagcgca ctttccgtga tgtttttagt tgggcttgta ttatttagat attcggcaaa
//
LOCUS       DBMDXF010000255         2507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_8981_length_2507_cov_1.722776, whole genome shotgun sequence.
ACCESSION   DBMDXF010000255 DBMDXF010000000
VERSION     DBMDXF010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8981_length_2507_cov_1.722776"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="ACZPD4_08335"
     CDS             <1..389
                     /locus_tag="ACZPD4_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR1379098.1"
                     /translation="EQTKKDIDKHLTECRECQKKMAAMKTQLDIQNTNVDLKINPLKK
                     VRFYQKILTVLGAVIAFILGACIPIAILGFTVLERGEIAAYQIERVKGLWYVLTSWSC
                     ASGIVVCVIYFLLILLIKKIIKKKGK"
     gene            complement(512..1345)
                     /gene="dapF"
                     /locus_tag="ACZPD4_08340"
     CDS             complement(512..1345)
                     /gene="dapF"
                     /locus_tag="ACZPD4_08340"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1379099.1"
                     /translation="MKFTKMQGIGNDYVYVNCLQETIENPSELAKKISDRHYGVGSDG
                     LIMINPSDKADFEMEMYNADGSRGEMCGNGIRCVAKYVYDYGLTDKTSISVETLAGIK
                     YLDLTVEDGKVVLVKVDMGKPMLRPEEVPVVSEKEEVIDEPITVDGQEYRMTCVSMGN
                     PHAVVFIDQDVKEFPLETVGVKFENHERFPKRVNTEFVNVLDRHTAQMRVWERGSGET
                     LACGTGACAVAVACALNGLTEDEVTVKLLGGDLQIKWDREKNTVYMTGPAEVVFDGEW
                     K"
     gene            complement(1445..2392)
                     /locus_tag="ACZPD4_08345"
     CDS             complement(1445..2392)
                     /locus_tag="ACZPD4_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR1379100.1"
                     /translation="MNEPDVMVYLMTGFLDSGKTGFLKFTLKQDYFQIDGKTLLILCE
                     DGEDEYDAMEMLRYGVVIERLEDREELTEEYLAELDRKHEPDRVVIEYNGMWKVSDFE
                     NMKLPKGWGIEQKITTVDASTFQTYLTNLKPLFVEMVRGAEMVLFNRCEDIKPLAGYR
                     RSVKVVSPQAEVIFEDEQGEVENIFEDDVPYDLDAPVIEIPREDYGIWYVDMMENPAR
                     YRGKVIEITAKVLKPSGFPSKVFLPGRMAMTCCADDTTFLGYVCRSAYAPKLKAGQWV
                     KVRAKVRFANVSVYRGEGPVLEAEHIEAAEPIEELVYFN"
BASE COUNT          663 a          667 c          386 g          791 t
ORIGIN      
        1 cggaacaaac gaaaaaagat attgataaac atttaaccga gtgcagagag tgccaaaaaa
       61 agatggccgc aatgaaaaca cagctagaca ttcaaaacac caacgttgac ctaaagatca
      121 atccattaaa aaaggtaaga ttttatcaaa aaatactgac agtcttggga gcggttatag
      181 cattcatttt aggtgcatgc attccgatcg ctatattagg gtttactgtt cttgaacgag
      241 gagaaatagc cgcttatcaa atcgagagag taaaaggcct ttggtatgta ctcacttcat
      301 ggagctgcgc ttcgggaatt gtagtatgcg taatttattt tttgctcata ctgcttatca
      361 aaaaaataat caaaaaaaag ggaaagtaaa aattatctca gtttttccgg acataaaaat
      421 acccccatat cggactttta tacatcattg tcctatatgg ggggcatcat atcagtcaaa
      481 gcggcagcct taaaatctgt caacaaagaa gctacttcca ctccccgtca aatacaactt
      541 ccgcagggcc tgtcatatat accgtattct tttctctgtc ccacttgatc tgcaaatctc
      601 cgccaagcag ttttactgtc acctcatctt ccgtcaatcc gttcaacgca caggcaacgg
      661 cgactgcgca tgctccggtt ccgcacgcca gcgtctctcc cgatccccgc tcccacacgc
      721 gcatctgcgc cgtatgacgg tcaagaacat ttacaaactc cgtattcacc ctctttggaa
      781 acctttcatg attttcaaat tttactccga ctgtttcaag cggaaattct tttacatctt
      841 gatctataaa tacgactgca tgaggatttc ccatagaaac acatgtcatc ctatattcct
      901 gtccatccac ggtaatcggt tcatctatca cttcttcctt ttccgaaaca acaggcactt
      961 cttccggacg cagcatcggt tttcccatat caactttgac aaggacaact ttcccgtctt
     1021 ctacagtcag atcaagatat ttgatacctg ccaatgtctc tacagaaata cttgttttat
     1081 ctgttaagcc gtaatcatat acatatttcg ccacgcagcg aatcccgttt ccgcacattt
     1141 ctccacgaga accgtccgca ttatacatct ccatttcaaa atcggcttta tcagatggat
     1201 tgatcataat aagcccgtcc gaaccgactc cgtaatgccg atcactgatc ttctttgcca
     1261 gttccgatgg attttctatc gtttcctgca aacagttcac ataaacataa tcatttccta
     1321 tgccctgcat ttttgtaaat ttcataggtt ttctcctttt ctctcgcgtt cctctttttt
     1381 tcttcaaatc cagtccgact cttctgccgg tcttcttttc gtcttttcaa tactcatttc
     1441 atctctaatt aaaatagaca agttcttcta tcggttctgc cgcctcgata tgttccgctt
     1501 cgagaacagg cccttctccg cgataaaccg atacattcgc aaaccgtact ttcgcccgta
     1561 cttttaccca ttgtcctgct tttaatttcg gagcgtacgc acttctgcat acatatccca
     1621 gaaatgtcgt atcatccgca caacatgtca tcgccatccg ccccggaaga aacacttttg
     1681 acggaaatcc ggaaggtttc aacaccttcg ctgtgatttc aataactttt ccacgatatc
     1741 gggcgggatt ttccatcata tccacatacc agataccata atcttctctc ggaatctcga
     1801 ttacaggcgc atccagatca tacggcacgt catcttcaaa aatattttcc acttctcctt
     1861 gttcatcttc aaatataact tccgcctgag ggctgactac tttgacgctt ctcctgtatc
     1921 ccgccagagg cttaatatct tcacagcggt taaaaagaac catctcggcg cctcttacca
     1981 tttctacaaa gagcggtttt aaattagtca gatatgtctg aaatgtgctt gcgtccactg
     2041 ttgtgatctt ctgctcgatc ccccagccct taggaagctt catattttca aaatcactga
     2101 ccttccacat tccgttatat tcaatgacaa ctcgatcagg ctcgtgcttt cgatctaatt
     2161 ccgccaaata ctcctctgtc agttcttctc tgtcttccag ccgctctatt acaacgccgt
     2221 atcttaacat ttccatcgca tcatactcat cttctccatc ttcgcaaaga atgagcaatg
     2281 tttttccatc gatctgaaaa taatcctgct tcaatgtaaa ttttaaaaat ccggtctttc
     2341 cactgtctaa aaatcccgtc atcaaataaa ccattacatc cggttcattc atgttttatt
     2401 tttccctcct tattttttca ttccatattt tccggttctc gctctttcat tcgtttcctt
     2461 tgtatgttcg ccgattaaag gccgaacagt ttttccagtt tttcttc
//
LOCUS       DBMDXF010000256         2486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9068_length_2486_cov_1.418029, whole genome shotgun sequence.
ACCESSION   DBMDXF010000256 DBMDXF010000000
VERSION     DBMDXF010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9068_length_2486_cov_1.418029"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(170..967)
                     /locus_tag="ACZPD4_08350"
     CDS             complement(170..967)
                     /locus_tag="ACZPD4_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1379101.1"
                     /translation="MNDQSNGQKLFLTRLRRHRITVLISRVLILTVFLFLWELCARFE
                     IIDSFIFSSPSKITLCFLEMLKNRSLFLHIGITLYETFISFILVILLSILAAILLWYS
                     KKLSEILEPYLVVLNSLPKSALAPLLIVWLGATSKTIIIAGMSVAIFGSILNLYTAFI
                     NVDQEKIKLIYTLHGNRRHALTKVVLPSSVPAIISNMKVNIGLCLVGVIIGEFLAAKS
                     GLGYLIIYSSQVFKMDWLLMSIILLCIMAMGLYALINVVEKRVLKKY"
     gene            complement(960..1736)
                     /locus_tag="ACZPD4_08355"
     CDS             complement(960..1736)
                     /locus_tag="ACZPD4_08355"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1379102.1"
                     /translation="MRPLLELNNISYSYHTIDGETKALSDISFQLAPGEFAAVVGPSG
                     CGKSTLLSLIAGLIEAENGTIFLDGEPSEKSFRKSAKIGYMLQHDHLFEWRSILKNVL
                     LGAEINKSVSQETKQRAKELLRQYGLKRFINSRPSELSGGMRQRAALIRTLMPNPELL
                     LLDEPFSALDYQTRLTVSDDIGKIIKHSGKTALLVTHDLSEAISLADRIIILTKRPAH
                     IARIVPIAFSIENDTPLNRRNAPEFKQYFNEIWKELNQDE"
     gene            1934..>2486
                     /locus_tag="ACZPD4_08360"
     CDS             1934..>2486
                     /locus_tag="ACZPD4_08360"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1379103.1"
                     /translation="MNVQKINYQKELDKILKRLEEEGRVPTLLIHSCCAPCSSYVLEY
                     LSRYFRITVLYYNPNIYPESEYTKRIIEQQKLIKDMRFQYPVSFLAGKYDKEKFYEMA
                     KGMEHLKEGGARCMKCYELRLEEAAQQAAEGRFDYFTTTLSISPLKNSQKLNEIGVRV
                     GKKYGVEYLVSDFKKRNGYKRSVE"
BASE COUNT          770 a          433 c          573 g          710 t
ORIGIN      
        1 tataaattta gtttgatttt tacatatgaa gaacaggtaa aatttgttaa gattattaac
       61 aatatcaaaa ataatgctct tgttacttaa atataaaaga tcaattaagt cggcaaagag
      121 actgctgctt gagtttgaag tttcaagcga cagtcttcta ttgccatatt taatactttt
      181 tcaacactcg tttttccact acattgatca gagcatacaa tcccattgcc ataatacaga
      241 gcaaaataat cgacatgaga agccaatcca tcttaaatac ctgactggaa tagatgatca
      301 gatatccaag tccgcttttg gcagcgagaa attctccgat aatgactccc acaagacaaa
      361 gtccgatatt gactttcata ttgcttatga ttgccggaac agaggaaggc agtacaactt
      421 ttgtaagcgc atgcctgcgg tttccgtgca gtgtatatat cagtttgatc ttttcctgat
      481 cgacattgat aaaggcggta tacagattca agatacttcc gaagatggcg acggacattc
      541 cggcaataat aattgttttt gaagttgcgc cgagccatac gatcagaaga ggggcaagcg
      601 cggatttcgg aaggctgttt agcacaacga ggtatggttc cagtatttcc gatagctttt
      661 tactatacca aagcaagatt gccgcaagga tgctgagcaa tatgacgagt atgaagctga
      721 taaatgtttc gtataaagta atcccgatat gaaggaaaag agatcggttt ttcaacattt
      781 ccaaaaaaca aagagtgatt tttgaaggac tgctgaagat aaaggaatct ataatttcaa
      841 aacgagcgca taattcccat aaaaaaagaa acacagttaa aataaggaca cggctgatca
      901 aaactgtgat cctatgacgg cgaaggcgcg ttaaaaaaag tttttgtccg ttagattgat
      961 cattcatctt gatttagctc cttccatatt tcgttaaaat attgtttgaa ttccggggca
     1021 tttcttcggt ttagcggggt atcgttttcg atggaaaatg caatcggaac aattcgggca
     1081 atatgtgcgg ggcgttttgt caatatgatg atccgatcgg caaggctgat tgcctctgaa
     1141 aggtcatggg ttacaagaag cgcggttttt cctgaatgtt tgatgatttt tccgatatca
     1201 tcgctgacgg taagacgtgt ttgataatcg agagcggaaa aaggttcatc taaaaggagc
     1261 agttcgggat tcggcatgag agtgcggatc agagctgctc tttgccgcat tccaccggat
     1321 aattcagacg ggcgggagtt aataaaacgc tttaatccgt attgacgcag aagttctttt
     1381 gcccgttgtt tcgtttcctg ggatacactt ttgttgattt cagcgccgag caggacgttt
     1441 tttaaaatgc tcctccattc aaaaaggtga tcatgctgaa gcatatagcc tattttggca
     1501 gattttctga aagatttttc cgatggttcg ccgtctagga agattgttcc gttctctgcc
     1561 tctataagtc ctgcgattaa cgaaagaagt gtggatttcc cacatccgga aggtccgaca
     1621 acggcggcaa attctccggg ggcaagctga aaagaaatat cggataaagc ttttgtttct
     1681 ccgtctattg tatgataaga ataactgatg ttgtttaatt ccagtaaagg tctcacagag
     1741 tcacttccgt tcttatttaa gtatctaaga ttaatgtatg agagccggcg gcagtgtgtg
     1801 aatatcgaca tatcggaact tgatctgatt tggaaatgaa acggattgac attgtaattt
     1861 ggaaaaagag atgatatctt attataagta gaataaacaa aaaggtaacg cagcatatag
     1921 gagaacagtt aagatgaatg ttcagaaaat caattatcaa aaggaattag ataaaatatt
     1981 gaaacgtctt gaagaggaag gccgtgtccc gactcttttg atccatagct gttgtgcgcc
     2041 gtgcagcagt tatgtgctgg aatatttatc ccgctatttc aggatcacgg tgctttatta
     2101 taacccgaat atttatcctg agagcgagta tacaaagcgg atcatagagc agcagaaact
     2161 gataaaagat atgcggtttc aatatccggt ctcattcctt gcgggaaagt atgataagga
     2221 gaagttctat gaaatggcaa aagggatgga gcatttgaaa gaaggcggcg cacgatgcat
     2281 gaaatgctat gaacttcggc ttgaagaggc ggcgcagcag gcagcggaag gcagatttga
     2341 ttattttacg acgactctga gcatcagccc tctgaaaaat tcgcagaagt tgaacgagat
     2401 cggagtgcga gtcgggaaaa agtacggcgt agagtattta gtttcggatt ttaaaaagcg
     2461 gaacggatat aaacggtctg tagaat
//
LOCUS       DBMDXF010000257         2462 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9146_length_2462_cov_0.914443, whole genome shotgun sequence.
ACCESSION   DBMDXF010000257 DBMDXF010000000
VERSION     DBMDXF010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_2462_cov_0.914443"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="ACZPD4_08365"
     CDS             <1..237
                     /locus_tag="ACZPD4_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1379104.1"
                     /translation="AGEDLRLRGPGDLFGIRQSGILDFKIADVFQDAAVLKIAGEEAN
                     KILKRDPELKDPSYKKIKNHIDHKIQQIMLETTL"
     gene            434..640
                     /locus_tag="ACZPD4_08370"
     CDS             434..640
                     /locus_tag="ACZPD4_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HIR1379105.1"
                     /translation="MANRSSNRAAVPEAKGALDKFKYEVASELGVPLTDGYNGDLTSK
                     QNGSVGGYMVKKMIEQQEKQMAGK"
     gene            762..1331
                     /gene="rsmD"
                     /locus_tag="ACZPD4_08375"
     CDS             762..1331
                     /gene="rsmD"
                     /locus_tag="ACZPD4_08375"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR1379106.1"
                     /translation="MRVIAGSARSLRLKTLDGMDTRPTTDRIKETLFNMIGPSMFDCV
                     FLDLFAGSGGIGIEALSRGAREAVFVENNPKAMMCVKDNLKFTKLEGKAVTLTTDVMN
                     ALYKLEGEKVFDYIFLDPPYDRGFEKRVLEYLSDSSLVYEDTQIIVEASKETDFSYLQ
                     DLGFEIIREKTYKTNKHLFIEKAGKEEVC"
     gene            1325..1822
                     /gene="coaD"
                     /locus_tag="ACZPD4_08380"
     CDS             1325..1822
                     /gene="coaD"
                     /locus_tag="ACZPD4_08380"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1379107.1"
                     /translation="MLRAIYPGSFDPVTYGHIDIMRRSCKIVDELIVGVLSNKAKIPL
                     FSVEERVKMLKEVTKDLDNIRIVPFDGLLVEFASRMNAGLVIRGLRAITDFEYELQMS
                     QTNQKLDPNIETMFLTTSIEYSYLSSTTVREIAAFGGDLTQFVPEAVALELEKKMNTK
                     GECNK"
     gene            1819..2460
                     /locus_tag="ACZPD4_08385"
     CDS             1819..2460
                     /locus_tag="ACZPD4_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1379108.1"
                     /translation="MSSRIEQIIEEIEEYIDRDCKFQPLSTSKIIVNKDQMDELLREL
                     RMKTPDEIKRYQKIIANKDAILADAQAKADSMIEEAQIHTSELVSEHEIMQQAYAQAN
                     EIVTAATEQAQEILDNATSDANEIRIGAMRYADNLMANAEGIIGHTLDSYTTKYDSLV
                     NSLQECYDMVRNNRAELDIPDQPVAAQNEDQGYEQEEEPAEDAFPDFDLDSEE"
BASE COUNT          844 a          393 c          609 g          616 t
ORIGIN      
        1 gccggggaag atctgcgtct tagaggaccg ggagatctgt tcggaatacg tcaaagcggg
       61 attcttgact ttaagattgc agatgtgttt caggatgcag cagtgttaaa aatcgccggc
      121 gaagaagcaa ataaaatttt aaaaagagat cctgaattaa aagacccttc ttataaaaaa
      181 ataaaaaatc atatcgatca taagattcaa cagattatgt tagaaacgac gttgtaatgg
      241 atgaaaggtg taaaaaattg ccagtttaaa aattgttgtc ctccatatac tatgagcgta
      301 acaaaacaaa cggaagcaaa tgtatcgtaa ccggctgcat agaaagcaga ttacatacat
      361 gtgcagccgg aagaattgtt acaacaaacg aaaacgataa atgtcgttaa aatgcaaaag
      421 gaggaaattg atcatggcaa atcgttcatc taacagagca gcagtaccgg aggcaaaggg
      481 cgctctggac aaattcaagt atgaagtggc aagtgagtta ggagtaccgt tgacagacgg
      541 ttataacgga gacctcacat cgaaacagaa cggatctgtt ggcggatata tggtcaagaa
      601 aatgatcgag cagcaggaaa aacagatggc aggcaaataa catcgttttt tcgataaaaa
      661 agctcgtccc tattacaaag aaaaagacgt ccgggattga cccggacgtc tttttctgat
      721 aaaattttgc gtagttgtta aagaaaacga ggtgtcattt tatgcgtgta attgcaggaa
      781 gtgcaagaag tctcagatta aaaactttag acgggatgga cacaagacca acgactgacc
      841 gtatcaaaga aacactgttc aatatgatcg gtccgtcaat gtttgactgt gtatttcttg
      901 atctctttgc gggaagcggc gggatcggaa ttgaagcgct cagcagagga gctcgcgaag
      961 ctgtttttgt agagaataat ccgaaggcaa tgatgtgtgt taaagacaat ctgaagttta
     1021 ccaaattaga aggcaaggcg gttacattga ctacagatgt tatgaatgcg ctttacaagc
     1081 tggaaggtga aaaggtattt gattatattt ttttggatcc tccgtatgac agaggattcg
     1141 aaaagagggt attagaatat ttatcggatt caagtcttgt ctatgaagat acccagatca
     1201 ttgtggaagc gtcaaaagaa actgattttt cgtatttaca ggatttggga tttgaaatta
     1261 tcagagaaaa aacgtataaa acgaataagc acctgtttat cgagaaggcg gggaaagagg
     1321 aagtatgtta agagcaattt atccgggcag ttttgatccg gttacttacg gtcatataga
     1381 tattatgcga agatcatgca aaattgttga cgaattaatt gtcggcgtat tgagcaataa
     1441 agcaaaaatc ccgttgtttt ctgtagaaga acgtgttaaa atgttaaaag aagtgacaaa
     1501 agatcttgat aatatccgaa tcgttccgtt tgatggactt ttagtggagt tcgcttcgcg
     1561 tatgaatgcc ggacttgtga taagaggact gcgggcaatc acagattttg aatatgaact
     1621 tcagatgtca cagacaaatc aaaaactgga tccgaatatt gagacgatgt ttttgacgac
     1681 gagtattgaa tattcttatt taagttctac aacagtaaga gagattgctg cttttggggg
     1741 agatttaaca caattcgtgc cggaggcggt agctttggaa cttgaaaaaa agatgaatac
     1801 aaaaggagag tgtaacaaat gagcagccgt attgagcaga ttattgaaga aatcgaggaa
     1861 tacatcgaca gggattgtaa gttccagccg ctgtccacat caaagattat cgtgaataaa
     1921 gatcagatgg acgagctgtt gcgcgaactt agaatgaaga ctccagatga aattaagaga
     1981 tatcagaaga ttatagcgaa taaagacgcg attttagcag atgcacaggc aaaagcagac
     2041 agcatgatcg aagaagcaca gattcacacc agtgaacttg tcagtgagca tgagattatg
     2101 cagcaggcat atgcacaggc gaacgagatt gtgactgctg ccacagagca ggcacaggag
     2161 attcttgata atgcaacaag cgatgcgaat gaaatccgta tcggagctat gcgttatgca
     2221 gataatctga tggcgaatgc agaaggaatc atcggacata cactggacag ttatacaacc
     2281 aagtatgata gtcttgtgaa ttctcttcag gagtgctatg acatggttcg taataaccgt
     2341 gcggagctgg atattccgga tcagccggta gcagcacaga atgaagatca gggatatgag
     2401 caggaagaag aaccagcaga ggatgcattc ccggattttg acctggacag tgaggagtag
     2461 at
//
LOCUS       DBMDXF010000258         2452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9187_length_2452_cov_1.287963, whole genome shotgun sequence.
ACCESSION   DBMDXF010000258 DBMDXF010000000
VERSION     DBMDXF010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_2452_cov_1.287963"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..61)
                     /locus_tag="ACZPD4_08390"
     CDS             complement(<1..61)
                     /locus_tag="ACZPD4_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1379109.1"
                     /translation="MTKMRVHELAKELGMENKEL"
     gene            complement(77..400)
                     /locus_tag="ACZPD4_08395"
     CDS             complement(77..400)
                     /locus_tag="ACZPD4_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR1379110.1"
                     /translation="MSKRDKVLSLIGLAMKAGKCVSGETMTESETKSGKAKFVIVAGD
                     ASENTKKKFGDMCKFYKVPFCFYGDKDTLGHAMGKEFRASLAILDEGFADGIQRELKN
                     REDIA"
     gene            complement(393..665)
                     /gene="rnpM"
                     /locus_tag="ACZPD4_08400"
                     /gene_synonym="ylxR"
     CDS             complement(393..665)
                     /gene="rnpM"
                     /locus_tag="ACZPD4_08400"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1379111.1"
                     /translation="MAVNKKIPMRKCVGCGEMKPKKELLRILRTETGEFVVDALGKAN
                     GRGAYLCRSVECFQKAVKNRGLERSFKQAIPPEVYERMEKEMGELE"
     gene            complement(686..1900)
                     /gene="nusA"
                     /locus_tag="ACZPD4_08405"
     CDS             complement(686..1900)
                     /gene="nusA"
                     /locus_tag="ACZPD4_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1379112.1"
                     /translation="MNIELMEALTILEKEKNSSKDVMMDAIENSLISACKNHFGTSDN
                     IKVIMDRDTCDYAVYAEKNVVEEVEDPALEISLEEAEKLDSALQVGDIAQIPVHSKEF
                     GRIATQNAKNLILQKIREEERKVVFDQYFEKEKDIVTGVVQRYVGKNISINLGRADAM
                     LTENEQVKGEHFKPTERIKLYVVEVKNTPKGPKILVSRTHPELVKRLFEAEVAEVRDG
                     TVEIKSIAREAGSRTKMAVWSNDPNVDPVGACVGMNGARVNAVVQELRGEKIDIINWD
                     ENPALLIENSLSPAKVISVMADPEEKIASVIVPDYQLSLAIGKEGQNARLAARLTGYK
                     IDIKSETQAIEAGELPENYMEEMGLVGEEGYTGDYEEEYNEEYSEESYDEAAEDQNEE
                     SEEIEFIETENE"
     gene            complement(1922..2389)
                     /gene="rimP"
                     /locus_tag="ACZPD4_08410"
     CDS             complement(1922..2389)
                     /gene="rimP"
                     /locus_tag="ACZPD4_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1379113.1"
                     /translation="MSKREEYEQKTEELLEPIVSALGFELVDVEYVKEAGTWYLRAYI
                     DKPGGITVDDCEAVSRKFSDILDEKDYIPDSYVFEVSSPGLGRPLKKEKDFKRSIGEE
                     VEIRTYRAIDRQKEFTGILKGFDENTVTIIYEDETEQVFDRAEIALIRLALDF"
BASE COUNT          570 a          652 c          356 g          874 t
ORIGIN      
        1 tgagctcttt attttccatt ccaagctctt tcgcaagttc atgtaccctc atttttgtca
       61 tattaaaact acctccttat gcaatatcct ctcggttttt cagctctctt tgaattccat
      121 ctgcaaatcc ttcgtcaagt atcgccagag atgcacggaa ctcttttccc attgcatgcc
      181 ctaaggtatc tttatctccg tagaaacaaa atggaacttt ataaaactta cacatatccc
      241 cgaatttttt cttcgtgttc tccgatgcgt caccggcgac aatgacaaac ttcgcttttc
      301 ctgatttcgt ctcgctttct gtcattgtct cgccgcttac acattttccg gccttcatcg
      361 ccagtccgat cagggaaagg accttatctc tcttactcaa gctctcccat ctccttttcc
      421 attctctcat atacttccgg cggtattgcc tgcttaaacg accgttccaa tccacggttt
      481 ttcacagctt tctgaaagca ttctacgctc cggcaaagat aagcgcctct gccgtttgct
      541 tttccaagtg catccacgac gaattctccg gtttctgtcc tgagtattcg cagcaattct
      601 tttttaggtt tcatttctcc acaccctaca catttacgca taggaatttt tttattaact
      661 gccaaataac ccactccttt agatattact cattctctgt ctcgataaat tcaatttctt
      721 ccgactcttc gttctgatct tctgccgctt catcataaga ctcttcactg tattcttcgt
      781 tatactcttc ctcgtaatct cctgtataac cttcttcacc gacaagtccc atctcttcca
      841 tataattttc tggaagttct cctgcctcta tcgcctgtgt ctcgctctta atatcaatct
      901 tatatcctgt aagtcttgca gcaagacgtg cattctgccc ttcttttccg attgcaagtg
      961 aaagctgata gtcgggaacg atcacactcg cgattttttc ttccggatct gccattacag
     1021 aaatcacttt tgcagggcta agtgaatttt ctatcagaag cgccggattc tcatcccagt
     1081 tgataatatc gattttttca ccgcgaagtt cctgaacaac tgcgtttact cgtgctccgt
     1141 tcattcccac acaagcgcct acgggatcta cattcggatc gtttgaccat acagccattt
     1201 ttgtacggga acctgcttct cttgcaatgc tcttaatttc aactgtaccg tctcttactt
     1261 ctgccacctc tgcttcaaaa agacgtttca caagttcggg atgtgtacgg gatacaagta
     1321 tcttcggtcc tttcggagta tttttcactt ctactacata cagtttaata cgttctgtag
     1381 gtttaaaatg ttcccctttc acctgctcgt tctcagtaag cattgcatct gctcttccaa
     1441 gattaatact gatatttttc ccgacatatc gctgaacaac acctgtcaca atatcttttt
     1501 ctttttcaaa atactggtca aatacaactt ttctttcttc ttctcttatt ttttgaagaa
     1561 tcaggttctt tgcattctga gtcgcaatac gcccaaactc ttttgagtgt accggaatct
     1621 gtgcgatatc gccgacctgt aatgcagaat ccagcttttc cgcttcttcg agactgatct
     1681 ccaacgcagg atcttctacc tcttctacta catttttctc tgcatataca gcataatcgc
     1741 aagtatcacg atccatgatt actttaatgt tatccgatgt tccgaaatga ttcttacatg
     1801 cgctgatcag cgaattttca atcgcatcca tcataacatc tttactgcta tttttttctt
     1861 tctcaagaat tgtcaacgct tccattaact ctatgttcat tttgtcattt cctcctaaat
     1921 tttaaaaatc caaagcaaga cgaatcaatg caatttctgc tctgtcaaat acttgttctg
     1981 tttcatcttc atatataatg gtcacagtgt tttcatcaaa ccctttcaaa ataccggtaa
     2041 attctttctg acgatcgatt gcccggtaag tacggatctc tacttcttct ccgatacttc
     2101 tcttaaaatc tttttctttt tttaacggtc tgccaagccc cggtgagctc acctcaaata
     2161 cataagaatc cgggatataa tctttttcat cgagaatatc cgaaaacttt ctgctgaccg
     2221 cctcacagtc atctaccgta atcccgcccg gtttatcgat atacgcacga agataccatg
     2281 ttccggcttc cttgacatat tctacgtcaa ccagttcaaa tccaagtgca gacacgatcg
     2341 gttctaaaag ttcttctgtt ttctgttcat attcttctct ttttgacacg ctatacttcc
     2401 tttcttctca tttggcagaa gctatctgcc gcttatgttt cttttgttat cg
//
LOCUS       DBMDXF010000259         2421 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9316_length_2421_cov_1.153017, whole genome shotgun sequence.
ACCESSION   DBMDXF010000259 DBMDXF010000000
VERSION     DBMDXF010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9316_length_2421_cov_1.153017"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="ACZPD4_08415"
     CDS             <1..805
                     /locus_tag="ACZPD4_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1379114.1"
                     /translation="ILLFGLTPFVSTYATDEKQYQWNTTSKNCSEIDLRNYFGKYEGS
                     FVLYDLKRDVWKIHNIDRAVLRVAPNSTYKIYDALFGLEEGVITPANSLIKWNGKNYP
                     FEAWNTDQTLQSAMGASVNWYFQTIDERLGADSIQKYLHQIGYGNENLSGNLSSYWLN
                     SSLKISPIEQVELLVKLHNNRFGFSLENTGTVKNALHISSSAFGDLYGKTGTGRVNGQ
                     DVNGWFIGFVENADNTYFFASNIQYQKHATGKKASEITLSVLSDLNIWR"
     gene            complement(807..2021)
                     /locus_tag="ACZPD4_08420"
     CDS             complement(807..2021)
                     /locus_tag="ACZPD4_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6070 family protein"
                     /protein_id="HIR1379115.1"
                     /translation="MLAMNLSGCSFFVKKDSGNTDIQSVRQESENADKTVMKRSKSEE
                     KEAEEILEICSKTYAKAEKEGKLDDLEMIRDLIKELGEKGFTAVDSENQVNMTEYEKL
                     IQFSEKAEAKKNGRMTVVEVAKGGSCIIRNMETHDGIVNMIRSYCIYENGKLKQSSED
                     IYRTESWEYTEDGHLMFSGRLAFEGSYEVTRNEIMEYMAYRVLSLDETCRELNRKYIL
                     PIGYERNNMFLTDWSEEEFAELDFYDMFDLFYQENHTANGEFTFGNAMGTSAVYLISK
                     EKFEPVIKSKFKISSEVLQSKTVYYPCEEVYEYKPRGLEETEYPEYPYPEVIGYVNND
                     DGTLTLKVQAVFPYRGVSKAYIHEVTVRDTKDGGIQYVGNRIIEPINNPEIRWHVPRL
                     TRTEWEELYGDN"
     gene            complement(2225..>2421)
                     /locus_tag="ACZPD4_08425"
     CDS             complement(2225..>2421)
                     /locus_tag="ACZPD4_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HIR1379116.1"
                     /translation="FTVEEVNLICIFECKSRTKVISDIKKAIKHLDDGEMVELSNRVV
                     AKLNNMTDKEFAVMEFVVTE"
BASE COUNT          680 a          569 c          338 g          834 t
ORIGIN      
        1 tattctcctt ttcggactta caccgtttgt ctctacctat gcgacagatg aaaagcaata
       61 tcagtggaac acaacttcaa agaattgttc tgaaatagac cttcgtaatt atttcggaaa
      121 atatgaaggt agttttgtat tatatgactt aaaaagagat gtctggaaaa tacacaatat
      181 agaccgagct gtcttacgag ttgctccaaa ctccacctac aaaatatatg atgctttgtt
      241 tggattagaa gaaggtgtca tcacccctgc aaattccttg atcaaatgga acgggaaaaa
      301 ctatcctttt gaagcgtgga acaccgatca aacattacaa tcagctatgg gagcttccgt
      361 caactggtat tttcagacta ttgatgaacg gcttggagcc gactctattc agaaatacct
      421 tcaccaaatc ggatatggaa atgagaattt aagcggtaat ctttcttcct attggttgaa
      481 ttcctccctg aagatttccc cgatagaaca ggtcgagctt ttagtcaaac tgcataataa
      541 ccgcttcgga ttctccttag aaaacaccgg gactgtaaag aacgccctcc acatctcctc
      601 ctctgctttc ggtgacctct acggaaaaac cggaacagga cgagtaaacg gacaagatgt
      661 caacggttgg tttatcggct ttgtagaaaa tgcagataat acttattttt ttgcttccaa
      721 tattcagtat caaaaacacg ccaccggtaa aaaggcttca gaaatcacct tatctgtttt
      781 atcggactta aatatatgga gataaactaa ttatctccat acaactcttc ccactctgtt
      841 cttgtcaagc gcggtacgtg ccatctaatc tccggattgt ttatcggctc gataatccga
      901 tttcccacat actgtatacc tccatctttc gtatcgcgca cagttacttc atggatgtac
      961 gcttttgata cccctctata aggaaaaaca gcctgtacct tcaatgtcaa agttccgtca
     1021 tcattattca catatccaat aacttccgga tacggatatt ccggatattc tgtttcttca
     1081 agccctctcg gtttgtattc atagacttct tcacacggat aataaactgt cttcgactgc
     1141 aacacttcac tgctgatttt gaatttagat ttgataaccg gttcaaattt ttcttttgaa
     1201 attaaatata ccgcgcttgt tcccattgca tttccaaaag taaactctcc gttcgccgta
     1261 tgattttcct gataaaacaa atcaaacata tcataaaaat ctagctctgc gaactcttct
     1321 tcactccagt ctgtcagaaa catattatta cgttcatatc cgatcggaag tatatattta
     1381 cggtttagtt ctctgcatgt ttcatctaat gacagtaccc tatatgccat atattccata
     1441 atctcatttc tcgtaacttc ataagatcct tcaaaggcta accgaccgga aaacatcaaa
     1501 tgtccgtctt ctgtatattc ccatgattct gtcctgtata tatcttctga agattgcttt
     1561 aatttaccat tctcataaat acaataactt ctgatcatat ttacaattcc gtcatgtgtc
     1621 tccatattcc gaatgatgca gctccctccc tttgcaactt ctacaacggt catgcgtcca
     1681 tttttctttg cttctgcctt ttcactaaac tgtatgagtt tttcatactc tgtcatgttg
     1741 acctgatttt cgctgtcaac tgccgtaaaa cctttctctc ctaattcttt aataagatct
     1801 cggatcattt ccaaatcatc cagtttacct tctttttcag ctttcgcata agtcttactg
     1861 caaatttcca gtatttcttc tgcttctttt tcttctgatt tacttcgttt cattactgtt
     1921 ttatccgcat tttcactttc ctgccgtact gattgaatgt ctgtgtttcc cgaatctttt
     1981 tttacaaaaa aactgcaccc ggacaaattc attgccaaca tgattccgct caccacaact
     2041 cctcttttcc aaattccttt tcttttcaaa ctatcgtcct ctctttttat tacacttgta
     2101 agattttaaa ctaaaaaaat ttctctcgtg ttaaatatta cacccgtaat atttttgtgt
     2161 caagctaata taatttatgc tggcaaaaca catgcatttc catgctctgc cagcaaaact
     2221 atttctattc cgttactaca aactccatca ctgcgaattc tttatctgtc atattattca
     2281 acttggcaac tacccgattt gacaattcta ccatttcacc atcatccaaa tgcttaatcg
     2341 cttttttaat atcacttata acctttgtcc tgctcttaca ctcaaaaatg cagattaaat
     2401 tgacttcttc tactgtaaat t
//
LOCUS       DBMDXF010000260         2403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9397_length_2403_cov_1.368375, whole genome shotgun sequence.
ACCESSION   DBMDXF010000260 DBMDXF010000000
VERSION     DBMDXF010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9397_length_2403_cov_1.368375"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="ACZPD4_08430"
     CDS             complement(<1..890)
                     /locus_tag="ACZPD4_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR1379117.1"
                     /translation="MSFSTILSAVIEGLEVAFIHVEADISNGLPVFHMVGYLSSEVKE
                     AGERVRTAIKNSGFEYPAKRTVVNLSPATMRKHGASFDLPIAAAILTALGQIEKPEKD
                     CLIIGELSLGGSVKKVSGVLPIVMEAKEKGVRKCIVPSENVTEASLVSEMEIVGAATL
                     QEAVEAMQNRRKRKKIQNKEITDCGNLRKTVQKETKFPDFADMKGQEFVKRAAEIAVA
                     GGHNLLLIGPPGSGKSMTAKCIAGILPPLDLRESLEITKIYSVLGLVDEKTPLIVDRP
                     FREVHHTAHASTASVSDLPPS"
     gene            1230..1850
                     /gene="sigK"
                     /locus_tag="ACZPD4_08435"
     CDS             1230..1850
                     /gene="sigK"
                     /locus_tag="ACZPD4_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HIR1379118.1"
                     /translation="MKSFPNPLTPSEEKYYIRKYTEGDLEAKHILIERNLRLVAHVIK
                     KYQNSEEDPEDLISIGTIGLIKAVVTFNPDKGNRLAAYASRCIENEILMYLRSKKKAA
                     KEISLYEPIGTDREGNEIKLYDIIETDEEEVAEKIHLKENIQTLYEKVEKELSQRERI
                     ILKMRYGLYNGEEYTQREIARQLGISRSYVSRIEKGAVEKLRKSFS"
     gene            complement(2031..2342)
                     /locus_tag="ACZPD4_08440"
     CDS             complement(2031..2342)
                     /locus_tag="ACZPD4_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR1379119.1"
                     /translation="MQEQYKVEILPTAWEDLKSIEDYYLLQFGVESAMKVTDQILNSI
                     ERLELYPNFGSLTPDKWLNRKGYHMIISERYVSIYRLIEKTVYIYHIADTQTEYTKLF
                     S"
BASE COUNT          738 a          561 c          383 g          721 t
ORIGIN      
        1 gatggtggca agtccgatac acttgccgtc gatgcgtgtg ccgtatgatg cacttcacga
       61 aacggacggt ctacaatcag cggggttttc tcatcaacta accctaatac actgtatatt
      121 tttgtaatct ccaaactttc tcgcaaatca agcggcggca atatcccggc aatacatttt
      181 gccgtcattg actttccact gcccggcgga ccgataagaa gaagattgtg ccctcctgca
      241 actgcgattt ccgctgctct ttttacaaat tcctgacctt tcatatctgc aaaatcagga
      301 aatttcgttt ctttttgaac tgtttttctc aaattcccac aatccgttat ctctttattt
      361 tgtatcttct ttctctttct tctgttttgc attgcctcaa ccgcttcctg caaagtcgca
      421 gctcctacaa tttccatttc actgacaagc gacgcttccg ttacattttc agacggaaca
      481 atacactttc tcaccccttt ttctttcgct tccattacga tcggcaaaac tccagacact
      541 ttcttcacac ttcctccaag actgagttct ccgataatca gacagtcttt ctccggcttt
      601 tcaatctgtc ctaatgcagt caatatcgcc gcggcgatcg gcaggtcaaa agacgcgccg
      661 tgctttcgca ttgtagccgg tgataaattc acaacagtcc gctttgccgg atactcaaat
      721 cccgaatttt taattgcagt tctcaccctt tctcccgctt cttttacttc cgatgacaaa
      781 taccccacca tatggaatac cggcaggccg ttcgatatat ctgcctctac atgaataaac
      841 gccacctcaa gaccttcaat caccgcagat aaaattgtac tgaaactcat ttgcactcct
      901 ttctatgagc ttcctgttct cctttgttaa aataacaaga tcttctatcg tttatactgt
      961 tgatttatac actatttatt atagaagaaa caccacttaa aataattgaa ttccaaccga
     1021 tccggtcaga cacctctttc agaggtcaga ttgccgaaga tgaatttctt cgggaatgta
     1081 gatttatgat aattcataaa aaaatgagcg tcaagaaaaa aatgaaaatt tcacgaaatg
     1141 acagaattta tttcccagtt tacagatgga aaagcacaaa caacatgtct tttacataga
     1201 ctatatcaaa tgtgctaaga ggtgttatct tgaaatcatt tccaaatccg ctcacgccgt
     1261 cggaagaaaa atattatatc cgaaaataca cagaaggtga cctcgaggca aaacacatcc
     1321 tgatcgaacg caatctaaga cttgtcgcac atgtaatcaa aaaatatcaa aattccgaag
     1381 aagatcccga agacctgatc tctatcggca caatcggact gatcaaagcc gttgtcactt
     1441 ttaatcctga caaaggaaac cgcctcgccg cctatgcttc cagatgcata gaaaatgaaa
     1501 ttcttatgta tcttcgttcc aagaaaaaag ccgcaaaaga aatctctctt tatgaaccaa
     1561 tcggcactga cagagaagga aacgaaataa aattatatga tattatcgag actgatgaag
     1621 aagaagttgc cgaaaaaatt cacctaaaag aaaatataca gacactttat gaaaaagtcg
     1681 aaaaagaatt atcgcagcgg gaacgtatta ttttaaaaat gcgttacgga ctctataacg
     1741 gagaagaata tacgcagcgg gaaattgcaa gacaacttgg aatttcccgt tcttatgtct
     1801 cacgaatcga aaaaggggct gtggagaaac tacggaaatc tttttcatga tttcaaaaat
     1861 acctatatac atgtaacaaa attgctaaca cttattttcg gtaatcaccg gaaacgccaa
     1921 aagcctctga aaaatccaga ctttgtctca tctcctagta aaagaactgc ctatcatcaa
     1981 tctttcaatg acaggcagtt cttctgctta aaattcttat tcaatttcat ttacgaaaac
     2041 agtttagtgt actctgtctg tgtatcggca atatgatata tataaactgt tttttctatc
     2101 agtcgatata tagaaacata cctttcagat ataatcatgt gatacccctt tcggttcaac
     2161 catttgtcag gtgtaagtga accaaaattc gggtataact ccaaacgttc aatactattt
     2221 aatatctgat ctgttacttt catcgcagat tccacaccga attgaagtaa ataatagtcc
     2281 tcaatacttt tcaaatcttc ccacgcagta ggaagaatct caaccttata ttgttcctgc
     2341 acgttctctc aattcctttc ttgcttcgga gatacttctt gtctttgctc cgtttaaccg
     2401 ttc
//
LOCUS       DBMDXF010000261         2347 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9626_length_2347_cov_1.825022, whole genome shotgun sequence.
ACCESSION   DBMDXF010000261 DBMDXF010000000
VERSION     DBMDXF010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9626_length_2347_cov_1.825022"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="ACZPD4_08445"
     CDS             <1..1064
                     /locus_tag="ACZPD4_08445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1379120.1"
                     /translation="GSVKRSGLLDRIFASLDEEGISYISLGGVVPNPRLSLVYEGIDL
                     CRREGVDFILAVGGGSVIDSAKAIGYGVENEGDVWDFYERKRQACGCLPIGVVLTIAA
                     AGSEMSDSSVITNENGWLKRGYNSSYCRPKFAVMNPELTMTLPKYQTASGCVDIMMHT
                     MERYFNHCDNMELTDGISEHLLRTVMKNAKILMDEPQCYKARAEVMWAGSLSHNGLMA
                     CGNDGGDWACHQLEHELGGMFDVAHGAGLAAVWGSWARYVCDALPDRFEQFAVNVMGV
                     ENGDDKNAVIQKGIEAMENFFRQIEMPTDLGGLNIEVSEDQMKELALKCSHFGKRTIG
                     CVKKLNQEDIYRIYQKAAD"
     gene            1202..>2347
                     /locus_tag="ACZPD4_08450"
     CDS             1202..>2347
                     /locus_tag="ACZPD4_08450"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1379121.1"
                     /translation="MESYMEMLANHAKHAAVAAAKLGTAEKNKGLCAVADELIMQQEL
                     ILAENQKDLKAAEEKGVKQSLIDRLALSEKRIADMAEGLRQIASLDDPVGEILSMKVR
                     PNGLRIGQKRVPLGVVGIIYESRPNVTADAFGLCFKTGNAVILRGGSDAIYSNQAIVR
                     VIKAGLRKEKLCQDLVLLVEDTSREVVNEMMKMHGLIDVLIPRGGAGLIANVVANSTV
                     PVIETGTGNCHVYVDETADISMAADIIENAKTQRMGVCNACESLVIHSGMLEKALLPI
                     VKKLSEHGIEIRGDERVREICPEIKAASQDDWGTEYLDAIISVKTVDSIDEAISHINK
                     YNTGHSESIITNDYNHALKFQDEIDAAAVYVNASTRFTDGFEFGFGAE"
BASE COUNT          699 a          390 c          682 g          576 t
ORIGIN      
        1 gcggaagtgt aaaaagaagc ggccttcttg atcggatctt cgcatcgctt gatgaggaag
       61 ggatttctta tatttctctt ggaggcgtcg ttccgaaccc cagattgtcg cttgtatatg
      121 aaggaatcga tctgtgccgg cgagagggcg tagattttat tctggcagtc ggaggcggaa
      181 gtgtgatcga ctcggcgaaa gcgattggat acggcgttga gaacgaaggg gatgtatggg
      241 atttttacga gagaaaacgt caggcttgcg gatgtctgcc aatcggagtt gtacttacga
      301 ttgcggcggc aggttcggag atgagtgatt cgtctgtgat cacaaatgaa aacgggtggc
      361 tgaaacgggg atacaacagc agttattgca gacctaaatt cgcggttatg aatccggaac
      421 tgacaatgac gcttccgaaa tatcagactg caagcggatg tgtggatatc atgatgcata
      481 cgatggagag atattttaat cattgtgaca atatggaact gacggatgga atcagtgaac
      541 atctacttcg tacagtgatg aaaaatgcga aaatcctcat ggatgaacca cagtgctata
      601 aagcaagggc agaagtaatg tgggcgggaa gcctttcaca taatggtttg atggcgtgcg
      661 gaaatgatgg cggagactgg gcgtgtcatc agcttgaaca tgagctcgga ggaatgtttg
      721 atgtggcgca cggcgcagga cttgcagcgg tatggggaag ttgggcgcgt tacgtttgtg
      781 acgctcttcc ggatcggttt gaacaatttg cggttaatgt tatgggcgtg gaaaacggtg
      841 atgacaaaaa tgcagtaata caaaagggaa tcgaagctat ggaaaacttt ttcagacaaa
      901 tcgaaatgcc gacggatctt ggcggattaa acattgaagt aagcgaagat cagatgaagg
      961 aacttgcttt aaagtgcagc catttcggaa agagaacaat cggatgtgtg aaaaaattaa
     1021 atcaggaaga tatttatcga atctatcaaa aagccgcgga ctgaacgatt acgtttaaag
     1081 attgttttac gaagaaaggg tggcataaaa gtgtgatagg tgatacaata agaaaaattt
     1141 gtagtagcta gccataataa tgcgtactgc ctgatgtcgg gcgggaagaa aggagcatat
     1201 gatggaatca tatatggaaa tgcttgcaaa ccatgcaaaa catgcggctg tcgcagcggc
     1261 aaaactggga acagcggaga aaaataaagg actttgcgca gtggcagacg agttgatcat
     1321 gcagcaggag ctgatccttg ccgagaatca gaaagatctg aaggcagcag aggaaaaagg
     1381 tgtgaaacag tctttgatcg accgattggc gctttctgaa aaaagaattg cagatatggc
     1441 ggaaggactc cggcagattg cctctcttga cgatccggtc ggagaaatat tatcgatgaa
     1501 agttcgcccg aacggactgc ggatcgggca aaagagagtt ccgctcggtg ttgtgggaat
     1561 catctatgag tcaagaccga atgtgacagc agatgctttt ggcctttgtt ttaagaccgg
     1621 aaatgcagtg atcctgcgtg gcggaagcga tgcgatctat tcaaatcagg caatcgtgcg
     1681 cgtgatcaaa gcaggattgc gcaaagaaaa actttgtcag gatcttgttc ttcttgttga
     1741 ggatacaagc cgggaagttg tgaatgaaat gatgaagatg cacggtttga tcgatgtgct
     1801 tatcccgaga ggaggcgcag gtttgatcgc aaatgttgtg gcaaacagta cggttcctgt
     1861 tattgaaaca ggaacgggaa actgtcatgt gtatgtggat gagacggcag atatcagtat
     1921 ggcggcagat attatcgaaa atgcaaagac acagaggatg ggagtctgca acgcatgtga
     1981 atcgcttgta attcacagcg gtatgttgga aaaggcgctg ctgcctatcg ttaaaaagct
     2041 gagtgaacac gggatcgaga tccgtggaga tgaaagagtt cgtgaaatat gtcctgagat
     2101 caaagcggct tcgcaagatg attggggaac ggaatatctt gatgcgatta tttcggtcaa
     2161 gacagtggat tcgattgatg aagcgatcag tcatattaat aaatataata caggacattc
     2221 tgaatcgatc atcacaaatg attataatca tgcgttgaag tttcaggatg agatcgatgc
     2281 ggccgctgta tatgtgaatg catctacacg ttttacagac ggttttgagt ttggtttcgg
     2341 ggctgag
//
LOCUS       DBMDXF010000262         2287 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9921_length_2287_cov_1.736048, whole genome shotgun sequence.
ACCESSION   DBMDXF010000262 DBMDXF010000000
VERSION     DBMDXF010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_2287_cov_1.736048"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(31..399)
                     /locus_tag="ACZPD4_08455"
     CDS             complement(31..399)
                     /locus_tag="ACZPD4_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HIR1379122.1"
                     /translation="MLEEVYKWIQNIAVYLVVVTAMLHAVPGKDYERYIRFFSGLVLI
                     VLIFMPVLSVFGMKETFGAIYSNQVYEKERKELEEAGNSYEEMLKNYIEENKEEEHTG
                     TKKADTDAGKESIKDGFEKG"
     gene            complement(490..1668)
                     /locus_tag="ACZPD4_08460"
     CDS             complement(490..1668)
                     /locus_tag="ACZPD4_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HIR1379123.1"
                     /translation="MKGRKGRFLFTLFLTLLVFGFGTRIVRADETDNFQSDKKSIETA
                     ETKILEEEFDFKEIEKSLSELFPKEKISFEEVVFSFLSGEPGEMLELLTGYIRDEIAY
                     EFLYSKKTLVYIILIAVMAAVFSNFAGAFQSRQISGISFYVVYMLLITLCLMSFRTAV
                     YGISEKLESLTTFMRVLCPGYFLAVAFSSGSATSIFFYNLILFLIYISELVIVRFLFP
                     VINVYIMVQMLGNLTEEDLFSEFADLLKKAVTWTLRTIVACIVGVNVVQGLLAPAIDT
                     VKRSALTRTAEALPWIGNVMGGMAEVTMGTIVLIKNGIGMAGAFIAVLICAVPILQML
                     LSALLYKMAAAAVQPVSDKRITACIRGVSEGYEMIVSVIFTVGFLFLLTIAVVAGVTT
                     "
     gene            complement(1665..2051)
                     /locus_tag="ACZPD4_08465"
     CDS             complement(1665..2051)
                     /locus_tag="ACZPD4_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HIR1379124.1"
                     /translation="MSMIQAGMIGVIGAMLAIQLKGSKAEYGIYVSVAVSIVLFVLIS
                     DRLGIFVRTVKELIAYAHLETSYFSVILKMVGITYIAEFASGICKDAGYQTIAGQIET
                     FAKLTILALGMPVLTALLETVREFLS"
     gene            complement(2114..>2287)
                     /gene="spoIIIAC"
                     /locus_tag="ACZPD4_08470"
     CDS             complement(2114..>2287)
                     /gene="spoIIIAC"
                     /locus_tag="ACZPD4_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HIR1379125.1"
                     /translation="KIAAVGILVSILSQVLKHSGREEQAFLASLAGLILVLSWVLPYI
                     YDLFRTVRQLFSL"
BASE COUNT          691 a          578 c          322 g          696 t
ORIGIN      
        1 aaatccagcc tgtgcagaag catttcaccg ctagcctttt tcaaatccat cttttatcga
       61 ctcctttccg gcatctgtat ccgctttttt tgttcctgta tgttcttcct ctttattttc
      121 ttctatataa tttttcagca tttcctcata agagttcccg gcctcttcca gttcttttct
      181 ttctttttca tatacctgat tactgtagat tgctccgaat gtttctttca tgccaaacac
      241 acttaagacc ggcataaaaa taagaacgat caatacaaga ccggaaaaaa aacgaatata
      301 tctttcataa tcttttcccg gtaccgcatg aagcatcgcc gtcactacaa caagatatac
      361 tgctatattt tgaatccatt tatatacttc ttcaagcatt tttgcctcct tttaacgaaa
      421 caaagcatac acatatgtat tttcagtcct gcactttttg ttctttatat agttatcatt
      481 tttcatattt catgtagtta ctccggccac aaccgctatt gtaagcaaaa ataaaaatcc
      541 tactgtaaaa ataacggaaa ctatcatttc atacccctcg ctcactccgc ggatacaagc
      601 ggtaattctt ttgtccgaaa ccggctgtac cgctgccgcc gccattttat acaaaagcgc
      661 agacaacaac atttgcagaa tcggaaccgc acatattaaa accgctataa aagctcctgc
      721 cattccgatt ccgtttttaa tcaaaacaat cgttcccatt gtcacttccg ccattcctcc
      781 catcacattt cctatccacg gaagtgcctc tgccgtcctt gtaagcgcac ttctttttac
      841 cgtatctatc gcaggtgcca acagcccttg cacaacattc accccgacaa tacatgcaac
      901 gatcgtacga agcgtccatg tcaccgcttt tttcaacaga tcggcaaatt cagaaaagag
      961 atcttcttcc gttaagtttc caagcatttg caccataata taaacattta tcaccgggaa
     1021 gagaaacctt acgatcacaa gctctgaaat ataaatcaga aaaagaatca aattataaaa
     1081 aaagattgaa gtcgcgcttc ccgacgaaaa cgccaccgca agaaaatatc cgggacaaag
     1141 aactctcata aacgtcgtaa gagactccaa tttttcactg attccataaa ccgccgtccg
     1201 aaaagacatc aaacaaagcg tgatcaaaag catatataca acgtaaaaac ttattcccga
     1261 aatctgtctg ctttgaaaag ctcccgcaaa attgctgaat acagccgcca taacggcaat
     1321 caatataata taaacgagag tttttttgct gtacaaaaac tcataggcta tctcgtctct
     1381 tatgtatccc gtaagcaatt ctaacatttc ccccggctct cctgacagaa aagaaaatac
     1441 aacttcttca aaggaaattt tctcttttgg aaacaattcc gatagacttt tttcaatttc
     1501 tttaaaatca aactcttctt ccaatatttt tgtttccgct gtttctatgc tttttttatc
     1561 cgattgaaaa ttatccgttt catctgcccg gacgatcctt gtcccgaacc caaatacgag
     1621 aagcgttaaa aataatgtaa aaagaaatct cccttttctt cctttcatga cagaaactcc
     1681 ctgactgttt caagcaaagc tgtcaaaacc ggcatcccaa gcgccaatat cgtcagcttt
     1741 gcgaacgttt ctatctgtcc cgcaatcgtt tgatatcctg catctttgca aattccggaa
     1801 gcaaattccg caatataagt aatgccgacc atcttaagaa ttacggaaaa atagcttgtt
     1861 tcaagatgag cgtatgctat aagttctttt acagtacgaa cgaaaatacc aagcctgtct
     1921 gagatgagca caaatagaac aatgcttacc gctacgctta cataaatccc gtattctgcc
     1981 ttgcttcctt ttaactggat cgccaacatt gcaccgatca ctccgatcat tccggcctgt
     2041 atcatgctca tctgctctca cctcttcctc ttctattttc attttctttt ctcatttccc
     2101 gcactttcgt ttactataat gagaataatt gtctgacagt acgaaaaaga tcataaatgt
     2161 aaggaagcac ccatgacaac actaaaataa gtccggccag acttgcaaga aatgcctgtt
     2221 cttctctgcc gctgtgtttt aatacctgac ttaaaataga aacaagtatt cctactgccg
     2281 caatttt
//
LOCUS       DBMDXF010000263         2285 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_9930_length_2285_cov_1.543956, whole genome shotgun sequence.
ACCESSION   DBMDXF010000263 DBMDXF010000000
VERSION     DBMDXF010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9930_length_2285_cov_1.543956"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="ACZPD4_08475"
     CDS             complement(<1..178)
                     /locus_tag="ACZPD4_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1379126.1"
                     /translation="MSSAKHFTEVLIGGKVYTLSGFEGEEYLQKVSSYLNHKITECTN
                     SEGYRKQSADTRNVL"
     gene            complement(277..1281)
                     /gene="ruvB"
                     /locus_tag="ACZPD4_08480"
     CDS             complement(277..1281)
                     /gene="ruvB"
                     /locus_tag="ACZPD4_08480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1379127.1"
                     /translation="MGKRIITTENLEEDIKIEGELRPQSLGEYIGQEKAKETLGIYIE
                     AAKARGETLDHVLFYGPPGLGKTTLAGIIANEMGVHMKVTSGPAIEKPGEMAAILNGL
                     QEGDVLFVDEIHRLNRQVEEVLYPAMEDFAIDIMIGKGSAARSIRLNLPKFTLVGATT
                     RAGMLTAPLRDRFGVMHRLELYTPEELKTIILRSASVLNVEIEEDGALELARRSRGTP
                     RLANRLLKRVRDFAQVKYDGKITKKVADYALDLLDVDKYGLDHIDRTILLTMIEKFQG
                     GPVGLDTLAAAISEDAGTLEDVYEPYLLKNGFIQRTPRGRIVTDLAYRHLGIESFADE
                     "
     gene            complement(1324..1938)
                     /gene="ruvA"
                     /locus_tag="ACZPD4_08485"
     CDS             complement(1324..1938)
                     /gene="ruvA"
                     /locus_tag="ACZPD4_08485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1379128.1"
                     /translation="MISYVKGELAAIEEQKAIIDVGGIGYGVFMPHQALAMLPSVGNE
                     VKLYTYLNVREDAMQLFGFLTKDDLEIFRLVIGVSGIGPKGGLNILSCMSPDELRFAI
                     MSGDAKMISSAPGIGKKTAEKLIVELRDKVDIEKMLDHAAHPEETIQTDMAKTSANEM
                     QSEAVQALVALGYGSAESLRAVKKTSVQCQTVEDILKEALQYLF"
     gene            complement(1943..>2285)
                     /locus_tag="ACZPD4_08490"
     CDS             complement(1943..>2285)
                     /locus_tag="ACZPD4_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1379129.1"
                     /translation="KEAFLLSVARVVLGGFIFSNLFSILYSLAGGILSLIVMGILKKT
                     GKFTVVGVSVCGGVFHNVGQLAVAMAVVQTYEVGYYFPVLLIAGLLTGMLIGMISAEV
                     LKRTKNLRLKE"
BASE COUNT          607 a          594 c          381 g          703 t
ORIGIN      
        1 gaagtacatt tcttgtgtca gcgctctgtt ttctgtatcc ttcactattt gtacattctg
       61 ttatcttatg attcaaatag gaagacactt tctgcagata ctcttcgccc tcaaatccgc
      121 tcaaagtata tacttttcct ccgatcaata cttccgtaaa atgttttgct gaactcatag
      181 cttcctcctc gcgtttctta aaattcatta taaactattt agcggtaaaa accaactact
      241 tttttcggaa ctatccgttt cataactatt ccgaaattat tcatccgcaa aagattctat
      301 cccgagatgc cggtatgcca gatcagttac tatgcgtcct ctcggagtcc gctgtataaa
      361 tccgtttttc aaaagatacg gctcatacac atcttccaat gttcctgcat cttctgaaat
      421 cgccgccgcc agtgtatcca aaccaaccgg accgccttgg aatttttcaa tcattgtcag
      481 cagaatcgtt cgatcgatat gatccagacc atacttatcg acatcaagaa ggtcaagcgc
      541 ataatctgcc acttttttcg tgattttgcc atcgtatttt acttgagcga aatctcttac
      601 acgtttcaac agacggtttg caaggcgggg agttcctctg gatcggcgag caagttctaa
      661 agccccgtcc tcttcgatct ctacatttaa cacagatgcc gaacgtaata tgatcgtttt
      721 tagttcttcc ggagtatata attccaaacg gtgcataaca ccgaaacgat cgcgtaaagg
      781 agctgttaac attcccgctc tcgttgttgc gccgacaagt gtaaatttag gcaggttcaa
      841 acggatcgag cgggcggccg acccttttcc gatcataata tcaatggcaa aatcttccat
      901 agcaggataa agaacctctt ctacttgtcg gtttaacctg tgaatctcat cgacaaacaa
      961 aacgtctcct tcctgaagac catttaagat tgccgccatt tctcccggtt tttcgattgc
     1021 aggtcctgac gttaccttca tgtgtacacc catttcattc gcaataattc cggcaagcgt
     1081 tgtttttcca agtcccggag gcccgtaaaa aagcacatga tcaagtgttt ccccgcgcgc
     1141 tttagcagct tctatataga tgccaagtgt ttcttttgcc ttttcctgac cgatatattc
     1201 gccgagagat tgcggacgca attctccttc aatcttaatg tcttcctcta agttttctgt
     1261 cgttatgatt cgttttccca ttgccgtcca ttatttcctt tccgccttat ccacatcctc
     1321 tcatcagaaa agatactgca gcgcttcttt caatatatct tctacagtct gacattgtac
     1381 agaagttttc tttaccgcgc gaaggctttc cgcactcccg taaccaagcg caacaagagc
     1441 ctgtaccgcc tccgattgca tttcatttgc cgatgttttc gccatatctg tctgtattgt
     1501 ttcttccgga tgagcagcat gatcaagcat tttttcaata tctactttgt ctcgaagttc
     1561 tacaattagt ttttcggcag tctttttccc gattccaggc gctgacgaaa tcatttttgc
     1621 atctcccgac atgattgcaa accgcagttc atccggagac atacatgaca aaatattcaa
     1681 accgcctttc ggcccgatcc cgcttactcc gatcacaagc cggaatattt ccaaatcatc
     1741 ttttgtcaaa aatccaaaaa gctgcatggc atcttcccgc acattcagat aagtgtaaag
     1801 tttcacttca tttcctacag aaggtaacat cgccagtgcc tgatgtggca taaacacacc
     1861 ataccctatg ccgcccacat caataattgc tttttgttcc tcgatcgcag ctaattcacc
     1921 ttttacatat gaaatcattt ttttattcct ttaatcgtaa atttttagtt cgtttcagta
     1981 cttcggcaga tatcattcct atgagcattc ccgtcaaaag ccccgcaatc aaaagtacag
     2041 ggaagtaata tcctacttcg tatgtctgaa ccacagccat ggcaaccgca agctgaccga
     2101 cattatgaaa aacaccgccg cagacgctga caccgactac agtaaatttc cctgtttttt
     2161 tcaatattcc cataacgatc agacttaaaa tcccgcctgc cagactatat aaaatagaaa
     2221 atagattact gaatataaaa ccgcctaaaa caacgcgggc aacagataag agaaacgctt
     2281 ctttc
//
LOCUS       DBMDXF010000264         2249 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10130_length_2249_cov_1.705307, whole genome shotgun sequence.
ACCESSION   DBMDXF010000264 DBMDXF010000000
VERSION     DBMDXF010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10130_length_2249_cov_1.705307"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="ACZPD4_08495"
     CDS             complement(<1..1331)
                     /locus_tag="ACZPD4_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR1379130.1"
                     /translation="MAKPIMIQGTMSNVGKSVLAGALCRILKEDGYKTAPFKSQNMAL
                     NSYITKEGLEMGRAQVMQAEAAGIEPSADMNPILLKPTNDTGSQIIVRGRPFGMMSAS
                     EYYKRKKEFIPSIMESYRKLDETYDVIVIEGAGSPAEINLKKDDIVNMGFAKMADAPV
                     LLVGDIDRGGVFAQLAGTMLLLEEEEKKRVKGTIINKFRGDVSILKPGLRMLEEKINT
                     SVLGVIPYFHLDIEDEDSLTERFRKKSRSGLAKIAVIRLPRISNFTDIAPLEAVDELD
                     VSYVSNISQFGDPDVVIIPGSKNTISDLLWMRQNGLEGKILRHAAKGGIVFGICGGYQ
                     MLGQSISDPTGAEQKGTVRGMGLLPVTTVFEQEKRRTRVSGRFAKVGGALAEMSGVCY
                     EGYEIHMGRTVLSKGTVPLVYMEEEGEQRQDGAQSDHVYGCYMHGIFDMSET"
     gene            complement(1234..>2249)
                     /locus_tag="ACZPD4_08500"
     CDS             complement(1234..>2249)
                     /locus_tag="ACZPD4_08500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1379131.1"
                     /translation="KCRKLREKLADRLRVDERMFAFGNGAAELLYTLVLAEKSKKALL
                     PIPSFSEYEQALKTVGCEIKYYQTKKNNGFCIDYDFLEELTEDVDIIFLCSPCNPSGK
                     KTDIELLKEAAKRCEQKKIRFVLDECFLEFEKEPESLFDKINIVHYPHLFLLRAFTKT
                     YAIPGIRLGYGLTSDEKLLENMEKMRQPWNVSVIAQEAGIAALDEKDRVEKMRRMIQI
                     ERMEIEKQLDILKIDFVSSDANFILIYSEINLFEELLQYGILIRDCANYRGLETGWYR
                     IAVKQRHENEQLLKALKEIIERNRQEADIDGKTDHDTGNDVECREECAGRRSLPHLEG
                     GRL"
BASE COUNT          563 a          612 c          361 g          713 t
ORIGIN      
        1 gtttcagaca tatcgaaaat cccatgcata taacagccat atacatgatc gctctgcgct
       61 ccgtcctgcc gctgttctcc ttcctcttcc atatagacaa gcggaacagt ccccttggac
      121 aaaaccgttc tccccatatg gatctcatac ccttcataac atacaccgct catttctgca
      181 agcgctccgc ctactttggc aaaccttcct gaaactcttg tccttctttt ttcctgttca
      241 aacactgtcg tcaccggcag aagtcccatc ccgcgaaccg ttcctttttg ttctgccccc
      301 gtcggatcgc tgatcgactg tccgagcatc tgatatcctc cgcaaatacc gaatacgatg
      361 cctccttttg ccgcatggcg cagtattttc ccttccaaac cgttttgcct catccacaaa
      421 agatcgctga tcgtattttt gcttcccggg atgatcacaa catcaggatc tccaaattga
      481 gaaatgtttg acacatagga cacgtcgagt tcatccactg cttcaagcgg agctatatcc
      541 gtaaaattag aaatcctggg aagacgtata accgcgattt ttgcaagacc tgatctgctc
      601 tttttccgaa accgctctgt caaactgtct tcatcttcaa tatcaagatg aaaatacggg
      661 ataactccaa gtacagaagt atttatcttt tcttccaaca tccgaagtcc gggttttaaa
      721 atcgatacgt ctccgcgaaa tttattgata atcgttcctt taacacgctt tttttcttct
      781 tcctcaagca ggagcatcgt tcccgcaagc tgtgcaaata caccgcctct gtcaatatct
      841 ccgacaagca gtaccggagc atctgccatt ttcgcaaacc ccatattgac aatatcgtct
      901 ttctttaaat tgatctccgc cggacttccc gctccttcga tcacaatgac atcatacgtt
      961 tcgtccaatt ttctgtatga ttccataatc gaaggaataa attctttttt ccgcttataa
     1021 tactcagacg cagacatcat tccgaaaggt ctgcctctga cgataatctg tgatcctgta
     1081 tcatttgtcg gttttaaaag gatcgggttc atatccgcac tcggctcgat tcctgccgct
     1141 tctgcctgca ttacctgcgc ccttcccatc tccagtccct cttttgtaat ataggaattt
     1201 aatgccatat tctgtgattt aaagggagct gttttataac cgtcctcctt caagatgcgg
     1261 caaagagcgc ctgccagcac actcttccct acattcgaca tcgttccctg tatcatgatc
     1321 ggttttgcca tctatgtccg cctcctgtct gtttctttct ataatctcct tcaatgcttt
     1381 cagcagttgt tcattttcat gtctttgctt cactgctatc cgataccacc ctgtttcaag
     1441 tccacggtaa tttgcacagt ctctgatcaa gatcccgtat tgcaaaagtt cttcaaacag
     1501 attgatttca ctatatataa gaatgaaatt agcatctgaa gaaacaaaat caatttttaa
     1561 tatatctaat tgtttctcta tctccatccg ttctatctgt atcatacggc gcattttttc
     1621 tacccggtct ttttcatcaa gcgccgctat gcccgcctcc tgggcgatca ccgacacatt
     1681 ccacggctgg cgcatcttct ccatattctc caacaatttt tcatcagatg tcaacccata
     1741 ccctaacctg atccccggga tcgcatatgt cttcgtaaaa gcccgaagca aaaacaagtg
     1801 aggataatga acgatgttta ttttatcaaa aagactttcc ggctcttttt caaattcaag
     1861 aaagcactca tccaacacaa aacgaatctt tttctgctca catcgttttg cggcttcttt
     1921 taggagttcg atatccgttt ttttccccga cggattgcaa ggagaacata aaaaaatgat
     1981 atccacatct tctgttagtt cttctaagaa atcataatct atacaaaaac cattattttt
     2041 tttcgtttga tagtatttta tttcgcatcc aactgttttt aacgcctgct catattctga
     2101 aaacgaggga atggggagga gagctttctt cgatttttct gccaggacaa gagtatagag
     2161 cagttctgcc gccccgtttc caaatgcaaa catcctctcg tccacccgaa gcctgtctgc
     2221 cagtttttcc cttaatttcc tacatttac
//
LOCUS       DBMDXF010000265         2244 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10155_length_2244_cov_2.434438, whole genome shotgun sequence.
ACCESSION   DBMDXF010000265 DBMDXF010000000
VERSION     DBMDXF010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10155_length_2244_cov_2.434438"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="ACZPD4_08505"
     CDS             complement(<1..709)
                     /locus_tag="ACZPD4_08505"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1379132.1"
                     /translation="MGMKINAHLPIPADLKAEYPLSSQILKLKEKRDREIREIFEGKS
                     DKFLVIVGPCSADNEDSVCEYVSKLATINEKVSDRLMIIPRIYTNKPRTTGEGYKGML
                     HQPKPDEAPDLLAGIIAIRKMHIRAIEESGLTGADEMLYPENRSYLDDILSYEAIGAR
                     SVENQQHRLTASAMDIPVGMKNPTSGDFSVMLNSVVAAQSSHSFIYRGMDVTTEGNDL
                     AHVILRGGVDKYGTCIPN"
     gene            complement(816..1169)
                     /gene="rnpB"
                     /locus_tag="ACZPD4_08510"
     ncRNA           complement(816..1169)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPD4_08510"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1188..1667)
                     /gene="rnhA"
                     /locus_tag="ACZPD4_08515"
     CDS             complement(1188..1667)
                     /gene="rnhA"
                     /locus_tag="ACZPD4_08515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR1379133.1"
                     /translation="MQVKIYTDGAARGNPDGPGGYGAILEYVDTKGTLHTKELSRGYV
                     RTTNNRMELMAVIAGLEALNRPCEVEVYSDSQYVVNAFNKQWVDGWIKKGWKRGKNEP
                     VKNPDLWKRLLKAKEKHKVSFHWVKGHDGHPQNERCDLLATSAADGDTLFVDEGVGK"
     gene            complement(1658..>2244)
                     /locus_tag="ACZPD4_08520"
     CDS             complement(1658..>2244)
                     /locus_tag="ACZPD4_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1379134.1"
                     /translation="YVFVSHRHHDHYNEEIFRLQEKFRDIQYIISSDVYELASHPEKN
                     DKIIYMKAGEEADIKGCYVRTLRSNDEGTAFFIRYKDQTIYHAGDLNWWHWEGEPDEY
                     NKNMRRSYQSEINKLCGEKIDAAFVPVDPRLGEQYCLGIDCFMKRTETKRVFPMHFWG
                     NYDVCSRLMLEECTKDYQDKIVKIEREGQTFLCR"
BASE COUNT          525 a          596 c          393 g          730 t
ORIGIN      
        1 agtttgggat acatgtcccg tatttatcta cgcctccgcg caatatgaca tgtgccagat
       61 catttccctc tgtcgtcaca tccattcccc tgtatataaa agaatgagaa ctctgtgctg
      121 ccactacaga attgagcatt acagaaaaat ctccgcttgt cggatttttc attcctacag
      181 gtatatccat cgcacttgcc gtcagacgat gctgctgatt ttccacagat cttgctccga
      241 ttgcctcata agacaaaata tcgtcaagat aacttctatt ttcgggatag agcatctcat
      301 ctgccccggt aagtccgctc tcctcaatcg ctcttatatg catctttcgg atcgctatga
      361 ttccggctaa cagatccgga gcttcgtccg gctttggctg atgaagcatt cctttatatc
      421 cttctcctgt tgttctcggc ttattcgtat aaattcttgg gatgatcatc aagcgatctg
      481 atactttttc gttgattgtc gccaatttcg atacatactc acatacggaa tcttcattgt
      541 ccgccgagca tggtcctaca attacaagaa acttgtctga tttcccttca aaaatctctc
      601 tgatttcccg atcccttttt tctttcagtt taagaatttg agaagaaagc ggatattctg
      661 ctttcaaatc agcaggaatc ggaaggtgtg catttatttt catacccata gttttttctc
      721 ctgtttcttt ttaaattagt atcctcttga cagatccgac atgtatttta gcgaatcaca
      781 gctttatatt gctaaagcat tcggggtaac aaaatgtgtt aagcaaaacg ataagccggg
      841 ttatgtcgtg gacaatcatc tatctaggca cgacgttgcc gccgtgctca agcgacctac
      901 ctgaagcgtg acgggccgcc acattgcttc tatccggtct tgcttcgaat ggggtttaca
      961 tgtgccctct ccgttaccgc agaggcggta gtctcttaca ctgcctttcc acccttacgc
     1021 cgatatggaa tccgacgcgg tttatttctg ttgcactggc ctgggagtca cctccaccgg
     1081 acgttatccg gcatcctgcc ctatgaagcc cggactttcc tcgtctgctg cctttcggca
     1141 cttacatccg cgattgtctg tcttacttaa cacagtatct gattatacta ttttcctact
     1201 ccttcgtcaa caaaaagtgt atctccatcc gctgccgaag tggcaagcag atcacacctc
     1261 tcattttgag gatgtccgtc atgtcctttc acccagtgaa acgacacttt atgcttttct
     1321 tttgctttga gaagacgctt ccaaagatcc ggatttttaa ccggttcgtt tttcccacgc
     1381 ttccatcctt tcttaatcca tccgtctacc cactgtttat taaacgcatt tacaacatac
     1441 tgtgaatcag aataaacttc tacctcacaa ggtctgttca acgcttccaa tcctgcgatc
     1501 actgccatca gttccattct gttgtttgtt gttctgacat atccccggga tagttctttt
     1561 gtatgaagcg tccctttcgt atctacatat tcaagaatcg ctccatatcc tcccggtccg
     1621 tccggatttc cccgtgccgc cccgtctgta tatattttta cctgcataga aatgtctgtc
     1681 cttccctctc gatcttcaca atcttgtcct gataatcttt tgtgcattct tcgagcatca
     1741 aacggctgca cacatcataa tttccccaaa aatgcatcgg aaatactctt tttgtttccg
     1801 ttcgtttcat gaaacagtcg attccgagac aatactgttc ccccagtctt ggatcgaccg
     1861 gaacaaacgc cgcatcaatc ttttctccgc aaagcttgtt gatctccgac tgataagaac
     1921 gacgcatatt tttattatac tcgtccggct ccccttccca gtgccaccaa ttcaaatccc
     1981 ctgcgtgata gatcgtctgg tctttatagc gaatgaaaaa tgccgttccc tcatcatttg
     2041 accggagcgt tcgcacataa catcctttta tgtccgcctc ttctcctgct ttcatataaa
     2101 tgattttatc gtttttctca ggatgagaag ccagttcata tacatctgaa gaaataatat
     2161 attgtatatc ccggaatttt tcctgcagcc tgaaaatttc ctcgttataa tgatcatgat
     2221 gcctgtgact tacgaaaaca taca
//
LOCUS       DBMDXF010000266         2193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10425_length_2193_cov_1.886233, whole genome shotgun sequence.
ACCESSION   DBMDXF010000266 DBMDXF010000000
VERSION     DBMDXF010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10425_length_2193_cov_1.886233"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      186..322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            438..1007
                     /locus_tag="ACZPD4_08525"
     CDS             438..1007
                     /locus_tag="ACZPD4_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1379135.1"
                     /translation="MEEETGTVKMVMSKKKTVKMVKMGVMVAISVAMVYLVHFPIFPA
                     VPFLEYDPADISILIGTFAFGPEAGLVLTVVTAVVQGLTVSAGSGLYGILMHMIATGV
                     LVLVSGMIYNRKKTKATAVFGLTAGMAAMAVVMVGANLLITPMFMGVSKDVVWKLMPF
                     IIGFNVIKAGINGAVTFLLYKRIAMFLRK"
     gene            1260..1961
                     /locus_tag="ACZPD4_08530"
     CDS             1260..1961
                     /locus_tag="ACZPD4_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR1379136.1"
                     /translation="MNYNKKRMIVALHLTVSSVMLLSGFTSPGTKMNTAGDLRYAGGE
                     IAVQIGDSVVDGTISMIKGTKQVSQSVQTAGDVWKTEAVRIEEMIRAEEERAAEEARI
                     AEEQAAQAAALEATQLEAANVMAGEQELLAALIYCEAGNQPYEGQVAVGAVVMNRVRS
                     GSYPNTISEVIYQSGQFGPAMTGWLDSVLSSGGYTETAMQAAADALAGSNPIGDCLSF
                     GNGNWGIQIGDHFFH"
     gene            1989..>2193
                     /locus_tag="ACZPD4_08535"
     CDS             1989..>2193
                     /locus_tag="ACZPD4_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1379137.1"
                     /translation="MDIKIRQYAEEDVKETIAIWNEVVKEGIAFPQKETLTESTGDLF
                     FKEQTFTGIAYDEEKKEIVGMYIL"
BASE COUNT          708 a          321 c          595 g          569 t
ORIGIN      
        1 atacatgaaa aagtgataaa agaaataaaa agatgataaa gtaaattaaa aaagtgacta
       61 cccataagaa aatttgtttt tttggaggat ttgtgcttgt gaaaatgtga aaatagaggt
      121 taaaaagaaa acaaagtcct tgaaaataga gaactataga tttataatga atagagtaaa
      181 tggaatacga tcttcggggt agggtgcaag tcccaaccgg cggtatagtc cgcgacccgc
      241 agcgatgcgg tggaatcggt gactttctta aacagaaaga attccgatac cgacagtata
      301 gtctggatga gagaagatag tgccgcttat tgctcaggaa agggcggtaa gttacacagg
      361 tgcagtttat cccgaacatg atatatgttc gggatttttt tataaaattg cagaaacgaa
      421 aaaaaggaga agaaaaaatg gaagaggaaa caggaacggt aaaaatggtg atgagtaaga
      481 agaaaacagt gaaaatggta aaaatgggag tgatggtagc catatcggta gcgatggtat
      541 atcttgttca ttttccgatt tttccggcag tgccgttttt agagtatgat ccggcggata
      601 tttctattct gatcggaaca tttgccttcg gaccggaggc tgggcttgtt ttgacagttg
      661 tgacggcggt tgttcaggga ctgacagtga gtgcggggag cggactgtat gggatattga
      721 tgcatatgat cgctacgggt gttcttgtcc tcgtatctgg aatgatctac aacagaaaaa
      781 agacaaaggc aacggctgtt tttggattga cggcgggaat ggctgcaatg gcagttgtta
      841 tggtcggggc aaatttattg attactccga tgtttatggg agtatcaaaa gatgtcgtat
      901 ggaaattaat gccgtttatt atcggcttca atgtgataaa agcgggaata aacggggcgg
      961 ttacatttct tttgtataag cggatcgcaa tgtttttgcg taaatgatca agtagatgaa
     1021 agtcttatct gtatctttgt tttgaaaaga tgcggatagg actgtttttt tgcggcagaa
     1081 aaagatgcag caaaaaatga gaaagaaaaa agttgacaac ttcttttctt tgtagtacaa
     1141 tcccagtcgt aacatttttg taatatctgt aataaatatg aaaacaacac atatatttta
     1201 aaaggggatt attaaaaata tattgtgaag atattgcaaa tgtttacgag gaggagtata
     1261 tgaattataa taaaaaaaga atgattgtgg cgctgcatct tacagtgtca agtgttatgc
     1321 tgctttcggg atttacaagt cccgggacaa agatgaatac cgccggagat ctccgttatg
     1381 cgggaggtga aatagccgtt cagatcgggg attcagttgt ggacggtacg attagtatga
     1441 tcaaaggaac gaaacaggtt tcgcaaagcg ttcaaacggc aggagacgta tggaaaacag
     1501 aggctgtccg tattgaagaa atgattcggg cagaggaaga aagagccgca gaagaagcaa
     1561 gaattgccga agaacaagca gcacaggcag cggctttgga agcgacacag ttggaagcgg
     1621 cgaatgttat ggcaggggag caggaacttc ttgccgctct tatctattgc gaagcgggaa
     1681 atcagccata tgaaggtcag gttgcagtcg gagcagttgt aatgaatcgt gtgagaagcg
     1741 gttcttatcc gaatacgatc agtgaagtga tctatcagtc tgggcagttc ggaccggcga
     1801 tgacagggtg gcttgattcg gttctttcgt cgggaggcta tacagagaca gcgatgcagg
     1861 cagcggcaga cgctcttgcg ggaagtaatc cgattggcga ttgcctttcc ttcggaaacg
     1921 gaaactgggg aatccagatc ggggatcatt tttttcatta aatcatagat acgaaagaag
     1981 gaaatagtgt ggatattaag atcagacagt atgcggaaga ggatgtaaaa gaaacgattg
     2041 ccatctggaa cgaagttgta aaagaaggga tcgcatttcc acagaaagag actcttacag
     2101 agtcgacagg agatctgttt tttaaagagc agacatttac ggggatcgct tatgacgaag
     2161 agaaaaaaga aattgtcgga atgtacattt tgc
//
LOCUS       DBMDXF010000267         2167 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10569_length_2167_cov_1.309777, whole genome shotgun sequence.
ACCESSION   DBMDXF010000267 DBMDXF010000000
VERSION     DBMDXF010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10569_length_2167_cov_1.309777"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(322..1698)
                     /gene="rlmD"
                     /locus_tag="ACZPD4_08540"
     CDS             complement(322..1698)
                     /gene="rlmD"
                     /locus_tag="ACZPD4_08540"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1379138.1"
                     /translation="MKKGQIFEGIIERVDFPNKGLVKVEEEGKDARYVTVKNAVPGQK
                     VRFIINKFKKGNAEGRLLEVLENSSLETRKPVCSIFPACGGCMYQTMPYEEQMKMKEG
                     QIRRIMDEAVDGEYLFEGVKASPKEFAYRNKMEFSFGDEYKDGPLSLGLHKKGSTYDV
                     LTASDCKLVHEDMNKILVCVLEYFKERKVSYYKKMQHVGYLRHLLLRRGDTTGEILVN
                     LVTTTQEEHDLSELTEQLLQLPLEGKIVGILHILNDSLSDVVKSDETRVLYGQDFFYE
                     KLLGLQFKITPFSFFQPNTKGAEVLYETVREYIGDIHDLTVFDLFSGTGTISQALAPV
                     AKKVIGVEIVEEAVEAAKENAERNGLSNCEFIAGDVFEVLDDIKEKPDVIILDPPRDG
                     IHPKALPKILDYGVDRIVYISCKMTSLARDLELIQQRGYTVEKMTAVDQFCETVHVET
                     AVLLTRKP"
     gene            complement(1913..2152)
                     /locus_tag="ACZPD4_08545"
     CDS             complement(1913..2152)
                     /locus_tag="ACZPD4_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379139.1"
                     /translation="MSKVEEFIAETKLNDLLHKKEAESQKNKVIWVLAIIGAVAAVAG
                     IAYAVYRFFTPDYLEEFEDDFDDDFDDYFSEDDQV"
BASE COUNT          587 a          559 c          296 g          725 t
ORIGIN      
        1 accgtatcat aaaacagtta aaggcctttg caaaggctca caactttgat aaaaactttg
       61 atccttaaat aattgacatc ttcacctaat cttgcataca gaccttttga cagcttactg
      121 tctgtattgc ctttctttta cttgtttttt attacctgct gatatagccc caacgattgc
      181 cccggctgaa gcgaacaata aaccacatat taaactcaca cagcgttacc ccatccccac
      241 ttccacggta acttatccat tttatttttc gtaaaaacac aactacattt ccatcaaata
      301 atcccgcaac cccgcataga atcagggttt ccgcgtgaga agcacggcgg tttcaacatg
      361 cacagtctca caaaattgat ccactgccgt cattttttca accgtatacc ctctctgctg
      421 tatcaactct aaatcccgcg ccaagcttgt cattttacat gaaatatata cgatacgatc
      481 cactccataa tccaagatct tcggtaatgc cttcggatgg attccatctc ttggcggatc
      541 taatatgatc acatccggtt tctcctttat atcatcaagc acttcaaaca catccccggc
      601 aataaactca caattagaaa gtccgttcct ttctgcattc tcttttgccg cttccactgc
      661 ttcttctaca atttctacgc caatcacttt ttttgccaca ggagcaagcg cctgactgat
      721 cgtgcctgtt ccgctgaaca gatcaaacac cgtaagatca tggatatcgc ctatgtattc
      781 gcgcactgtt tcataaagca cttccgcccc ctttgtattt ggctgaaaga aggagaacgg
      841 tgtgatctta aattgtaatc cgagcagttt ttcataaaag aaatcctgtc catataaaac
      901 cctcgtctca tcgcttttta caacatctga caaagaatca ttcaaaatat gaagaatacc
      961 tacgattttc ccttcaagcg gaagctgtaa aagctgttct gtcagttctg ataagtcatg
     1021 ttcttcctgc gttgttgtga caagattgac aaggatctct cctgtcgtat ctccccgtct
     1081 taaaagtaaa tgtcttaaat atccgacatg ctgcatcttt ttataataac tgactttccg
     1141 ctctttaaaa tactctaata cgcacacgag aattttattc atatcttcat gcacgagttt
     1201 acagtcggat gcggtcagca catcataagt acttcctttt ttatgaagtc cgagcgacaa
     1261 aggaccatct ttatattcat ctccgaatga gaattccatc ttatttctat atgcaaattc
     1321 tttcggactt gccttcacac cttcaaacaa atactcgccg tccacagctt catccataat
     1381 tcttcggatc tgtccttctt tcattttcat ctgttcttca tacggcattg tctgatacat
     1441 gcatccaccg catgccggaa aaatactgca tacaggtttc ctcgtttcaa gagaggaatt
     1501 ttccagaact tctaacagcc tgccttctgc attccctttt ttaaatttat ttataatgaa
     1561 tcgcactttt tgccccggta ctgcattttt tacagtcaca tatctggcat ctttaccttc
     1621 ttcttcaact ttcacaagtc ctttattcgg aaaatctact ctttcaatta ttccttcaaa
     1681 aatctgccct ttcttcataa gcttttcctt tcataataat ttttcttttt aacacagcag
     1741 aaaattaccg cattaactct tactctttta tcaatttttc tgttaacttt tatgtcaatc
     1801 cttcatccat atatcattgc aaagcagaca ccttttcaac tgcaaaacag gcagggtgca
     1861 gtttcatcca cactctgcct gtatattctg ttttactttt gcaaacagtt tcttacacct
     1921 gatcgtcttc gctgaaataa tcatcgaagt catcgtcaaa atcatcttca aactcttcca
     1981 aataatccgg tgtaaagaaa cgatacactg cgtaagcgat tcctgcaact gctgccactg
     2041 ctccaataat tgccaatacc cagatcactt tatttttctg actttccgcc tcttttttgt
     2101 gcagcagatc attcagtttt gtctcggcga taaattcttc tactttgctc ataatctacg
     2161 tcctttc
//
LOCUS       DBMDXF010000268         2144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10710_length_2144_cov_1.169359, whole genome shotgun sequence.
ACCESSION   DBMDXF010000268 DBMDXF010000000
VERSION     DBMDXF010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10710_length_2144_cov_1.169359"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            171..428
                     /locus_tag="ACZPD4_08550"
     CDS             171..428
                     /locus_tag="ACZPD4_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1379140.1"
                     /translation="MKEFKYVVTDPEGIHARPAGILVKQAAGYTSSVKITKGDKTADA
                     KRIFGVMGLGVKTGEEVTITVEGDDEDKAFAELEAFFKENL"
     gene            456..>1073
                     /locus_tag="ACZPD4_08555"
     CDS             456..>1073
                     /locus_tag="ACZPD4_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HIR1379141.1"
                     /translation="MITIKGKSVFGGVSIGKIMFYKRNEKVIKRTHVDDVDAEWKRFC
                     DAKDTAVSQLKELYDKAIEDVGEANAMIFEIHQMMLEDLDYLESIENIIRTQEVNAEF
                     AVATTADNFAQMFAAMDDAYMQGRAADVKDVSERVLDILCGVSGGMKEMTEPCIIAAD
                     DLAPSETVQLDKSKVLGFATMYGSSNSHTAILARTMNIPAVIGLGE"
     assembly_gap    1074..1083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1084..>1827
                     /locus_tag="ACZPD4_08560"
     CDS             <1084..>1827
                     /locus_tag="ACZPD4_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR1379142.1"
                     /translation="YDGKMAVIDGFTGMLYIDPDEETMKVMEEKRAKDQEQKALLEQL
                     KGKENVTKSGQKINVYANIGNVSDVGAVLKNDAGGIGLFRSEFLYLENSDFPTEEQQF
                     AVYKQVAENMAGKKVIIRTLDIGADKQVDYFGLDKEENPALGYRAIRICLTRKEIFKT
                     QLRALYRAAMFGNISIMFPMIISVAEVHEIKAIIAEVKEELKNEGIPFKDDVELGVMI
                     ETPASVMISRELAKEVDFFSVGTNDLTQYT"
     assembly_gap    1828..1837
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1838..2082
                     /locus_tag="ACZPD4_08565"
     CDS             <1838..2082
                     /locus_tag="ACZPD4_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR1379143.1"
                     /translation="AIDRQNAKLDKFYDPHHPAVLAMIKMAADNAHAEGAWIGICGEL
                     GADLELTEEFLKMGLDELSVSPAMVLPLRKRIRECE"
BASE COUNT          720 a          313 c          548 g          543 t
ORIGIN      
        1 tgtattaaaa aatgagtttt caataccggc taagtttagc atggttaaca gcaaaagtca
       61 acggatgatt gataaaagtg tgacagaaag attgaaaaat cagtgaatat gttatatact
      121 gtaaaaagta caacaaaagt acgatgaaat cattaaggag gactaagaga atgaaagaat
      181 ttaaatacgt tgtaacagat ccggagggaa tacacgcaag accggcagga attcttgtaa
      241 aacaggcagc aggatataca tcatccgtta aaattacaaa aggtgataaa acagcagatg
      301 caaagagaat cttcggagtg atggggcttg gcgtaaaaac aggcgaagaa gttacgatta
      361 cagtagaagg tgacgatgaa gataaggcat ttgcagaatt agaggcattt tttaaagaaa
      421 atttataaat cggattaagg gagaatttat aaattatgat tacaataaaa ggaaaaagtg
      481 tatttggcgg cgtttctatt ggaaagatca tgttttataa aagaaatgaa aaagtgatca
      541 aaagaacgca tgtagatgat gtcgatgcag agtggaagag attctgcgat gcaaaggata
      601 cggcagtatc ccagttgaaa gaattgtacg ataaagcaat agaagatgtc ggagaggcaa
      661 atgcgatgat tttcgagatc catcagatga tgcttgagga tcttgattat cttgagtcta
      721 ttgagaatat catccgtaca caggaagtga acgcagaatt tgcagttgca acaacagcag
      781 ataattttgc acagatgttt gctgcaatgg acgatgctta tatgcaggga agagcggcgg
      841 atgtaaaaga tgtgtctgag cgggtattgg atattttgtg cggagtatcc ggcggtatga
      901 aagagatgac tgagccgtgt attatcgcgg cagatgatct ggctccgagt gagacggtac
      961 agcttgataa gagcaaagtt ctcggttttg ctacaatgta tggatcttcc aattcacata
     1021 cggcaattct tgcccgtaca atgaatattc cggcggtgat cggactcgga gaannnnnnn
     1081 nnntatgacg gtaaaatggc ggtgattgac ggatttaccg gaatgttata cattgatcct
     1141 gatgaagaaa caatgaaagt catggaagaa aagcgcgcga aagatcagga gcagaaagcg
     1201 cttcttgagc agcttaaagg aaaagaaaat gtaactaaga gcggacagaa gatcaatgtg
     1261 tatgcaaaca tcggtaatgt ttcagacgta ggagcagtat tgaaaaatga tgcgggcggt
     1321 atcggtttgt tccgaagcga gttcctgtat ttggaaaatt cggatttccc gacagaagag
     1381 cagcagtttg cagtgtacaa acaagtcgct gaaaatatgg caggcaagaa agtaatcatt
     1441 cgtacactgg atatcggtgc ggacaagcag gttgattatt tcggacttga taaagaagag
     1501 aatccggctc tcggatatcg agcaatccgt atttgtttga cgagaaaaga gatctttaag
     1561 acgcagctta gagctcttta tcgtgcggcg atgtttggaa atatctctat tatgttcccg
     1621 atgattattt cagttgcgga agttcatgaa attaaagcca tcattgcaga ggtgaaagaa
     1681 gagcttaaaa acgagggaat cccgtttaag gatgatgtgg aactcggcgt tatgattgag
     1741 acaccggcat ctgttatgat cagtcgtgaa ttggcgaaag aagtggattt cttcagtgtg
     1801 ggaacaaacg atcttacaca gtatacannn nnnnnnntgg cgattgaccg tcagaatgca
     1861 aaattggata agttttatga tccgcatcac cccgcagtac ttgctatgat caagatggcg
     1921 gcggataatg cccatgcaga aggtgcttgg atcggtattt gcggtgaact tggagcagat
     1981 cttgaattga cagaagaatt ccttaagatg ggattagatg agctgtcagt atctccggct
     2041 atggttctcc cgcttagaaa gcgtatcaga gagtgcgaat aaagcaaata taaattgcat
     2101 ataaagagca gataaaaagc tgatttttaa atggtaagtt tgaa
//
LOCUS       DBMDXF010000269         2138 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10740_length_2138_cov_1.905253, whole genome shotgun sequence.
ACCESSION   DBMDXF010000269 DBMDXF010000000
VERSION     DBMDXF010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_2138_cov_1.905253"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="ACZPD4_08570"
     CDS             <1..374
                     /locus_tag="ACZPD4_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR1379144.1"
                     /translation="DKQRRVVVDFPFDAVADGTADAILSYVRARKNSQKSANVETMYQ
                     LVDEKVEALEFIVKNETNYTGITLKDLKVKPNNLIACIVRKRQIIIPNGNDSIEAGDS
                     VIVVTMEKHLEDLSDIIAQVQ"
     gene            376..1821
                     /locus_tag="ACZPD4_08575"
     CDS             376..1821
                     /locus_tag="ACZPD4_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1379145.1"
                     /translation="MNKKMIVYILGKMLGTEGLLLLIPAVVSLLYKEKTGVYFLVVAA
                     VLFALYLGLGRKKPENKQIYGKEGFVIVGLAWILWSLFGALPFFLSDCIPNYIDAVFE
                     TVSGFTTTGSTILQNIEALPKGMNFWRCFTHWIGGMGVLVFVMMITSLDTDNSMSLMR
                     AEVPGPEMDKLVPKVRHTAKLLYQMYFVLTIAEVIFLMFGGMNLYDAVVHSFSTAGTG
                     GFSNRNASVAFYDSAYIDGVITVFMILFGINFNLYFLLLLKDWKSVLKNEELRVYLGV
                     VAAAIVTITINILSMYETVAHAFRYASFQVGAIITTTGFYTADFEQWPELSKTILMML
                     MVIGACGGSTGGGIKVSRFMILCKSIRQEIHKMLHPNAVTMVRMNGKKVGADTMRSIN
                     IYFSAYVFIIIVSVLLVSLDNFDFATSFSGVLTTINNVGPGISQVGPTDNFHAFSALS
                     KIVFSFDMLFGRLEIFPYLLILSPDLWRKRF"
     gene            1865..>2138
                     /locus_tag="ACZPD4_08580"
     CDS             1865..>2138
                     /locus_tag="ACZPD4_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1379146.1"
                     /translation="MKKIGGKIAVVLAVIALLGVYYYVALPAVNIHATEFWIFLVILV
                     VLAALIYVRKKKLNRYEMKESKGLKAILTVLVVIVAVYLLGSLLSSP"
BASE COUNT          581 a          352 c          558 g          647 t
ORIGIN      
        1 ttgacaagca acggcgggtc gttgttgatt tccctttcga tgcggtcgcg gatggaacgg
       61 cagatgcgat tttaagttac gtcagagcga gaaagaactc ccaaaagagc gcaaatgtag
      121 agacgatgta ccaacttgtg gacgaaaaag tagaagcatt ggagttcatt gtgaaaaatg
      181 aaacgaatta tacaggtata acgttgaaag atttgaaagt gaaaccgaat aatctgatcg
      241 catgtatcgt aagaaagcgt cagattatca taccgaatgg aaatgacagc atagaggcag
      301 gagacagcgt gattgttgtt acgatggaaa aacatctgga agatctttcg gatattatag
      361 cgcaggtaca gtaagatgaa taaaaagatg atagtgtata tactggggaa aatgcttggt
      421 acggaagggc ttttgcttct tataccggcg gtggtgtctc tcctttacaa agaaaaaacg
      481 ggagtttatt ttcttgtagt ggcggcggta ttatttgctc tttatcttgg attgggaagg
      541 aaaaagccgg agaacaaaca gatctacggg aaggaaggct ttgtgatcgt cggacttgcg
      601 tggattttat ggtcgttgtt cggagcgctt ccgttctttt tgtcggattg tattccgaat
      661 tatattgatg cggtttttga aacggtttcc ggttttacga cgaccggatc tactattttg
      721 cagaatatag aggctcttcc gaaaggaatg aatttctggc ggtgctttac acattggatc
      781 ggcggaatgg gagtgctcgt ttttgtgatg atgatcactt ctcttgatac ggataattct
      841 atgtcgctta tgcgggccga agtgccggga ccggagatgg ataaactcgt tccgaaagtt
      901 cgtcatacgg caaaactgtt gtatcagatg tattttgttc tgacgatcgc ggaagtgatc
      961 ttccttatgt ttggcggaat gaatttgtat gatgctgtcg ttcattcgtt cagtacggcg
     1021 ggaacaggag gattctccaa cagaaatgcg agcgttgcat tttatgacag cgcatatatt
     1081 gacggcgtga tcaccgtgtt tatgatcttg ttcggaatca actttaatct ctactttctg
     1141 ctgttgctta aagactggaa gagcgtgttg aaaaatgaag agcttagagt gtatctcgga
     1201 gtagttgccg cggcgatcgt aacgattacg atcaatatat tgagtatgta tgaaacagtg
     1261 gcgcacgcgt ttcgctatgc ctcgttccag gtaggggcga tcattacgac aacgggattt
     1321 tatacggccg attttgaaca gtggcccgag ttatctaaaa cgatactgat gatgcttatg
     1381 gtgatcggag catgcggggg atcaacggga ggcggtatta aagtaagtcg ttttatgatc
     1441 ctttgcaaat cgatccgtca ggaaattcat aagatgcttc atccgaatgc ggtgacgatg
     1501 gttaggatga acggaaagaa agtaggcgca gatacgatgc gcagcattaa tatttatttt
     1561 tcggcatatg tatttatcat tattgtgtca gtgctgcttg tttcgcttga taattttgat
     1621 tttgctacat cattcagcgg agttttgaca acaataaaca acgtaggacc gggaatttca
     1681 caagtaggcc ctaccgataa tttccatgcg ttttctgcgc tgtcgaagat tgtgtttagt
     1741 tttgatatgc tattcggaag attggaaata ttcccttatc ttctgatact gtcacctgat
     1801 ctttggcgaa agaggtttta gagagaagct ttaaaatggg attccagaaa ggtaagaaaa
     1861 gtatatgaaa aaaataggtg gaaagatagc ggttgtactg gcggtgatcg cgttgcttgg
     1921 tgtttactat tacgtagctc ttcctgcagt taacattcat gcgacagaat tttggatctt
     1981 ccttgtgatt cttgttgttt tggcagcgct gatctatgtc aggaagaaaa agttaaacag
     2041 atacgagatg aaggaatcga aaggattaaa agcgattctt acggttcttg tagtgatcgt
     2101 agcggtatat ctgttgggat ctcttctttc ctctccga
//
LOCUS       DBMDXF010000270         2110 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_10906_length_2110_cov_1.376804, whole genome shotgun sequence.
ACCESSION   DBMDXF010000270 DBMDXF010000000
VERSION     DBMDXF010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10906_length_2110_cov_1.376804"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(41..817)
                     /locus_tag="ACZPD4_08585"
     CDS             complement(41..817)
                     /locus_tag="ACZPD4_08585"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIR1379147.1"
                     /translation="MGKILKYMTTIALAAVMLFGTMTTAFAVENSGQVVGNDQKGSIT
                     IHKYKMPDAENAFESNTENGGIEMDSLPETAEPLANVKFQVTKMEQDQAGKWNETTVS
                     RTVVTNESGEAVLEDLPLGRYKVEELGLDSSDGSDAVLPNEKDDAMVGKAFYVDVPMT
                     QADGQTLNYNVHVYPKNEVLSIEKDVTYVGNKHDSFDMQENQTWIIHTAIPGNIALTN
                     DNGSYDTAKLYKVTDKIDSQLTYPLDETLRTGNISSVSLS"
     gene            complement(822..>2110)
                     /locus_tag="ACZPD4_08590"
     CDS             complement(822..>2110)
                     /locus_tag="ACZPD4_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIR1379148.1"
                     /translation="FELNSDPVFFSVGGGEMEITGGEQTVTPTEGESTSAGENSTYID
                     RYSPDVNIKVTPGTGMVLDQIIVTYFDRKTQKNTGKTFTNETGEAAKNAAEFINKGKG
                     DEKEAGVIDGTVTVQPIYRLKANLTVGNDREKTDFVFKKVSAEDQTVMEGVRFKLTCN
                     HKHDKKCKGLISPESCTHAHNDKTGFVDEQGCTWSAEEVSKADGIVKFSELVSGEYIL
                     SEEATLDGFILPEGTWTVTVDAHKDEVKVEKTEKDDSTVPQFEKKDGIFQVENEPTVP
                     FSFIKVDEKTGNELAGAEFTLYQGDENDADKWSIVGGPVVSSEEGIVDFSRLKQGEYF
                     LKETKAPKGYLKPTGYWKVTIAVTDDASKKVTFEARGDVPAISGNHIEGFKVSNRETS
                     LLPKMGGNGTKIFVFSGFMIICCSLVLYFRFKRKGA"
BASE COUNT          518 a          536 c          328 g          728 t
ORIGIN      
        1 cgatgattca gtctaccaat atcgatggcg cttatttgga ctatgataaa ctgacagatg
       61 aaatatttcc ggtgcgtaat gtttcgtcaa gtggatatgt aagctgagaa tcgattttat
      121 ctgtcacttt gtataatttt gcggtatcat agctgccgtt atcatttgtc aatgcaatat
      181 tacccggaat cgccgtatgg ataatccacg tctgattctc ttgcatatca aaagaatcat
      241 gcttatttcc tacatatgta acatcctttt caatagacag cacttcattc tttggatata
      301 catgtacatt ataattaagt gtctgaccgt ctgcctgcgt catcggaaca tcaacataaa
      361 atgcttttcc taccatcgca tcatctttct cattcggaag aaccgcatca cttccatcgg
      421 atgaatccaa acctaactct tctactttgt atctgcccaa cggaagatct tccaaaactg
      481 cttctccgga ctcattcgta actaccgtac gagatactgt cgtttcgttc cattttcccg
      541 cttggtcctg ctccattttt gtaacctgaa acttaacatt tgccagcggt tctgccgttt
      601 caggaagact atccatttca ataccgccgt tttctgtgtt tgattcaaac gcattttctg
      661 catcaggcat cttatattta tgaatcgtaa tagaaccttt ctggtcatta cccactactt
      721 gtccgctatt ttccacagca aacgccgtcg tcattgttcc aaacaacatt actgctgcca
      781 gagctatcgt tgtcatatat ttcaaaatct ttcccataat tttaagctcc ttttcttttg
      841 aatctaaaat ataatacaag tgaacaacag atgatcataa atccactgaa cacaaaaatc
      901 ttcgtaccgt ttcctcccat cttcggaagg aggcttgtct ctctgttaga aaccttaaat
      961 ccttctatat gatttccact gattgccggt acgtcgcctc ttgcttcaaa agtgactttc
     1021 ttactggcat catctgtaac agcaatagtc accttccaat atcccgtcgg tttcaaatat
     1081 cctttcggag cttttgtctc tttaagaaaa tattcccctt gtttcaaccg actgaaatca
     1141 acaattcctt cttctgatga tacaaccgga cctcctacaa tgctccattt gtcggcatcg
     1201 ttctcatcac cctgataaag ggtaaattcc gcgcccgcaa gctcatttcc tgttttttca
     1261 tctactttta tgaaggaaaa cgggactgta ggttcatttt ccacttgaaa aattccatct
     1321 tttttttcga actgcggaac tgtggaatcg tctttttctg ttttttctac tttaacttca
     1381 tctttatgtg catcgacggt tactgtccat gttccttccg gcaaaatgaa tccatccaat
     1441 gtcgcctctt ctgataatat gtattcgccg gatacaagtt ctgaaaactt cacgattcca
     1501 tcagccttgc ttacctcttc cgcgctccat gtacatcctt gttcatccac gaatcccgtc
     1561 ttatcgttat gtgcatgtgt acaagactcc ggtgaaatca agcctttgca ctttttatca
     1621 tgtttatgat tacatgttag tttaaaccga actccttcca tcacagtttg atcttctgcg
     1681 cttacctttt taaaaacaaa gtccgttttt tctctgtcat ttccgactgt aagattcgcc
     1741 ttcaatctgt aaatcggttg aactgttacc gtaccgtcaa tcaccccggc ctctttttca
     1801 tcaccttttc ctttgttaat aaactcagcg gcgttttttg ccgcttctcc cgtctcattt
     1861 gtaaaggtct tcccggtatt cttctgcgtt ttccggtcaa aatacgttac aatgatctga
     1921 tctaacacca tccctgttcc cggagtaact ttaatattta catccggcga atatcgatcg
     1981 atatatgtac tgttttcccc ggctgacgtg gattctcctt ctgtaggcgt taccgtctgt
     2041 tctccgcctg tgatttccat ttcaccgccc ccgaccgaga aaaagacagg atcactgttt
     2101 aactcaaaac
//
LOCUS       DBMDXF010000271         2078 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_11111_length_2078_cov_1.541224, whole genome shotgun sequence.
ACCESSION   DBMDXF010000271 DBMDXF010000000
VERSION     DBMDXF010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11111_length_2078_cov_1.541224"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            212..556
                     /locus_tag="ACZPD4_08595"
                     /pseudo
     CDS             212..556
                     /locus_tag="ACZPD4_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(727..1341)
                     /locus_tag="ACZPD4_08600"
     CDS             complement(727..1341)
                     /locus_tag="ACZPD4_08600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1379149.1"
                     /translation="MDSIIFDVDGTLWDSADSVAAAWNLAIKKFSSLDITLDREILGS
                     VFGKTMTDIGDILFPSLSETEKDSLLSACCELENNYLKNHPGIIYEGVADTIEKLSKK
                     YPLFIVSNCQCGYIEATMQAIGIEHFIKDHLCFGETGVSKGQTLRMLIDRNHLSSPVY
                     VGDTQGDADACHAAGIPFIFAEYGFGNVPDAKMTIHKFSDLLNL"
     gene            1461..>2078
                     /locus_tag="ACZPD4_08605"
     CDS             1461..>2078
                     /locus_tag="ACZPD4_08605"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR1379150.1"
                     /translation="MRKKIVLIDGHSILNRAFYGVPDLTTPEGLHTNAVYGFLNIMFK
                     ILDEEKPEYLTVTFDVHAPTFRHEMFSEYKGTRKPMAEELRQQVPVIKEVLEAMNIST
                     IEKAGLEADDLLGTLSRMCEESGMDVSIISGDRDTLQLATEHVKIRIPKTKQGKTEIE
                     NYYAADVVERYKVTPREFIDVKALMGDSADNIPGVPGIGEKTATKI"
BASE COUNT          671 a          358 c          502 g          547 t
ORIGIN      
        1 gaacaatcaa taagtttgag tatgtttcgt attgcagaaa tgacagcatc tgtcgtaacc
       61 aatcggggaa agggtggtca aatcaagcga ggcggaaagg aaagaacaca tggacacaag
      121 tagtctaatg gagcagatac tttctggtga taacctcaat aaaacatatc tgcaagtcgt
      181 acgaaacaaa ggtgcagagg gagtggacgg aatgaagtac acagaacttc aagaacatct
      241 tgaaaagaac ggagaaaaca tcaaggacca gttgaggtca agaaaatata aacctcagcc
      301 ggtacggaga gtggagatcc caaagccgga gggtggcgtc agaaacctgg gagtaccaac
      361 agtaacagac agatttatac aacaagctat agcgcaggta ttaacaccaa tctatgagga
      421 acaattcaga ccaagcggac tgaaatacct tgggtttgga ttctgctttg acacaagagc
      481 acaccagttt aaggctaagt cacatgcaaa atcagttgtg aagtttaaga gaagaatgaa
      541 agtacttact tgtcgaagag actaactgca tttggattag tctcaatgtt agattactac
      601 accgaaaggt gtgttacttg ttaagttgat tgaaccgccg tgtaccggat ggtacgcacg
      661 gtggtgtgag aggtcgggga tttattaaaa tcccctccta ctcgatttat gaaaaatatt
      721 tggtaattac aaattaagca gatcggaaaa tttgtgaatg gtcatttttg catcaggaac
      781 atttccgaat ccgtattcgg caaaaataaa cggaatacct gcagcgtggc aggcgtctgc
      841 atcgccttgt gtatctccga cataaacagg agatgacagg tgatttcgat cgatcagcat
      901 gcgaagcgtt tgtcctttag aaacgcccgt ttctccgaaa caaagatgat cttttatgaa
      961 atgttcgata ccgattgcct gcattgttgc ttcgatatat ccgcattgac agttgctgac
     1021 aataaaaaga gggtattttt tagaaagctt ttcaatagtg tcagcgactc cttcataaat
     1081 gattccggga tgatttttta aataattgtt ttcgagctca cagcaggcgg aaagcagaga
     1141 atctttttcc gtttcggaaa gtgaggggaa aagaatatct ccgatgtcgg tcattgtttt
     1201 cccgaaaaca cttccgagaa tttccctgtc aagtgtaatg tcgagggaag agaacttttt
     1261 gattgccaaa ttccatgcgg cggcaacaga atcggcagaa tcccatagtg tgccgtctac
     1321 gtcaaaaatt atgctgtcca tgtatataat tcctttcatt tgtatgatca tatgataact
     1381 atagcataaa atcaaccttt taaaaagagg ctttttagtg tatacttgag tatgattgtg
     1441 aaacggaggt aattgctgcc atgcgaaaga aaatcgtatt gatagatgga catagtattt
     1501 tgaatcgtgc attttatgga gttcccgatc tgacaactcc ggaaggtctg catacgaatg
     1561 ctgtgtacgg gtttttgaat attatgttta aaatattaga tgaggagaaa ccggagtatc
     1621 tgactgttac atttgatgtt catgcgccga catttcgtca tgagatgttt tcggagtata
     1681 aaggaacgag aaaaccgatg gcagaagaac ttcgtcagca agttcccgta attaaagaag
     1741 tgcttgaagc aatgaatatt tctacaatag aaaaagcggg gcttgaagcg gatgatctgt
     1801 tgggaacgct ttcacgcatg tgcgaagaaa gcggaatgga tgtatcgatc atatcaggag
     1861 acagagatac gctgcagctt gccactgaac atgtgaagat tcggattccg aagacgaagc
     1921 agggaaaaac agagattgag aattattatg ctgcagatgt tgttgaacga tataaagtaa
     1981 cgccgagaga atttatcgat gtgaaagctt tgatggggga ttcggcggat aatattccgg
     2041 gagttccggg gatcggcgaa aaaacagcaa caaaaatt
//
LOCUS       DBMDXF010000272         2075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_11124_length_2075_cov_2.230496, whole genome shotgun sequence.
ACCESSION   DBMDXF010000272 DBMDXF010000000
VERSION     DBMDXF010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11124_length_2075_cov_2.230496"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="ACZPD4_08610"
     CDS             complement(<1..679)
                     /locus_tag="ACZPD4_08610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1379151.1"
                     /translation="MKRILMMVLRNIILVPFMWCKLCYHAHHVDKYTEEEHYKMLKFI
                     VRRANKGGNVTIAAEGLDNIPKENGFMFFPNHQGMYDVLAIMDVCPVPISVVAKKEVA
                     NIQFLKQVFACTKAYMIDREDVRQAMQVIMNVAKEVKAGRNYIIFPEGTRSRNGNKIG
                     TFKGGSFKAATKARCPIVPVALIDSFKPFDTKSISKVTVQVCFLPPLYYDEYKDMKTT
                     EIAAIVQE"
     gene            complement(676..1227)
                     /locus_tag="ACZPD4_08615"
     CDS             complement(676..1227)
                     /locus_tag="ACZPD4_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HIR1379152.1"
                     /translation="MDRHISAPVGRETAEELRAGDYVYITGTIYTARDAAHKRMYETL
                     REGGDLPINIEGQAIYYMGPSPAREGRPIGSAGPTTASRMDKYTPELLDLGMGAMIGK
                     GKRSQEVIDAVVRNKAVYFAAVGGAGALLSKCITSSEIVAYEDLGTEAIRKLTVVNLP
                     VIVVIDSKGNNLYETAVKEYRKI"
     gene            complement(1283..2074)
                     /locus_tag="ACZPD4_08620"
     CDS             complement(1283..2074)
                     /locus_tag="ACZPD4_08620"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR1379153.1"
                     /translation="MEANYSLSSDMVKAMRKAEEKEESVLGKQILAQLQDNLEIAASD
                     MIPICQDTGMAVVFLEVGQDVHFEGGSFEDAVNEGVRRGYTEGFLRKSVVGDPILREN
                     TKDNTPAVIHTRIVEGDRVKITVAPKGFGSENMSRVFMLKPAEGLEGVKNAILTAVKD
                     AGPNACPPLVVGVGIGGTFEKCALMAKKALTREVGKHSDIPYVRKLEEEMLEKINCLG
                     IGPGGLGGTVTALAVNVNTYPTHIAGLPVAVNICCHVNRHTVCVL"
BASE COUNT          512 a          585 c          334 g          644 t
ORIGIN      
        1 tttcctgaac tatcgctgcg atttccgtcg ttttcatatc tttgtattca tcataataaa
       61 gcggaggcaa aaaacaaacc tgaaccgtca ctttactgat agacttcgta tcaaacggtt
      121 taaacgaatc aatcagcgcc accggaacga ttggacaacg tgcctttgtt gccgctttaa
      181 aacttccacc tttaaatgtt ccgattttat ttccgtttct cgaacgagtt ccttccggga
      241 aaataatata atttcttccc gccttcactt cttttgctac attcataatg acctgcattg
      301 cctgccgcac atcttctctg tcaatcatat atgcttttgt acaagcaaac acctgcttta
      361 aaaactgaat atttgcaact tctttttttg caacaaccga gatcggaacc ggacacacat
      421 ccataatagc caaaacatca tacattccct gatgattcgg gaaaaacata aatccatttt
      481 ctttcggtat gttatccagc ccttcagccg ctattgtcac atttccgcct ttgtttgctc
      541 tgcggacaat aaacttcagc atcttataat gctcttcctc cgtatactta tctacgtgat
      601 gcgcatgata acaaagcttg caccacataa acggaacaag aattatattt cggagcacca
      661 tcatcaatat tcttttcata ttttccgata ctcctttacc gctgtttcat aaagattatt
      721 tcccttagag tcaatcacca ctatcaccgg aagatttact actgtaagtt ttctaatcgc
      781 ttctgtacca agatcttcat aagcaacaat ttccgatgat gtaatacatt ttgacagcag
      841 cgcgcccgcg ccgcctacag ctgcaaaata aaccgcttta ttgcgaacaa cagcatcgat
      901 cacttcttga cttctttttc cctttccaat cattgccccc attccgagat ccaataattc
      961 gggagtatat ttatccatac ggcttgccgt tgtcggacct gccgatccga tcgggcgtcc
     1021 ttcccgtgcc ggagacggtc ccatgtaata tatcgcctgc ccttctatat taataggtaa
     1081 gtctcctccc tcacgaagcg tttcgtacat ccgcttatgc gcagcatcac gcgctgtata
     1141 tatcgtgcct gtaatatata cataatctcc tgcccttagt tcttctgccg tctctctgcc
     1201 gacaggcgct gaaatatgtc gatccatttc tgtttccttt cacttctgcc tttttccact
     1261 gctgctatct tatccgctgt ttttacaaaa cacagactgt atgcctgttc acatggcagc
     1321 atatattcac cgcaaccgga agtcccgcaa tatgagtcgg atatgtgttg acatttactg
     1381 ccaaagccgt caccgtgcca cccaatccgc ccggtccgat gccaagacag ttgatcttct
     1441 ccaacatttc ttcttcgagt tttctcacat atggaatatc cgaatgtttt cccacttctc
     1501 gcgtaagcgc tttcttcgcc attaaggcgc atttttcaaa tgttcctccg attccgaccc
     1561 caactaccaa cggcgggcaa gcatttggtc ccgcgtcttt gacagctgta agaatcgcat
     1621 tttttactcc ttcaagtcct tccgccggtt tcagcataaa cacacggctc atattctcac
     1681 ttccgaaacc tttcggagct accgtgatct tcactcgatc accttccacg atccgcgtat
     1741 gaataaccgc aggagtatta tcttttgtgt tttcccgcaa aatagggtct cctacgactg
     1801 actttctcaa aaaaccttcc gtatagcccc ggcgcacccc ttcgtttacc gcatcttcga
     1861 aacttccgcc ttcaaaatgc acatcttgtc ctacctcaag aaacacaact gccattcctg
     1921 tatcctggca gatcggtatc atatccgaag cggcaatttc aagattatcc tgaagctgtg
     1981 ccagaatttg ttttccaagc acggactcct ctttttcctc tgcttttcgc atcgctttca
     2041 ccatatccga agaaagcgag taatttgcct ctatg
//
LOCUS       DBMDXF010000273         2072 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_11154_length_2072_cov_1.232369, whole genome shotgun sequence.
ACCESSION   DBMDXF010000273 DBMDXF010000000
VERSION     DBMDXF010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11154_length_2072_cov_1.232369"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="ACZPD4_08625"
     CDS             complement(<1..251)
                     /locus_tag="ACZPD4_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1379154.1"
                     /translation="MHINPNIEKPIFIQIAEQLEDSIFTKVFPEETKIPSTNELSSLL
                     NINPHTVLKGMNLLVDEEIIYKKRGLGMFVKKGEVNKIL"
     gene            398..1444
                     /locus_tag="ACZPD4_08630"
     CDS             398..1444
                     /locus_tag="ACZPD4_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1379155.1"
                     /translation="MISNKNRRDNDERKVLNNKKNGMLVLILTTLLYLLSIAVCVVGA
                     MIGNPLLLGISIFWMCVGWFPYCGLRVLKPQEALVLTLFGKYTGTLKGEGFYAVNPFC
                     TSVNPAADTHLNQSGDVDNSTRKSSLSGLLAGTSEKSGLESAGKKISLKIMTLNNSRQ
                     KINDCLGNPVEIGIAVMWRVVDTSKAVFNVDNYKEYLSLQCDTALRNIVRVYPYDVSP
                     NVDTTGDGVADEGSLRGSSEVVAARIRDEIQKRVSEAGLEILEARITYLAYAPEIAAV
                     MLQRQQASAIIDARKMIVDGAVGMVEMALDRLSEKKVVELDEERKAAMVSNLLVVLCG
                     NKDAQPIVNSGSLY"
     gene            1447..1650
                     /locus_tag="ACZPD4_08635"
     CDS             1447..1650
                     /locus_tag="ACZPD4_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin"
                     /protein_id="HIR1379156.1"
                     /translation="MADQKKKQIPLRLSAKLYDAVAAWAEDDFRSVNGQIEYLLTECV
                     RQRKKNGKYVSEHLDEHPEFDIK"
     gene            1873..>2072
                     /locus_tag="ACZPD4_08640"
     CDS             1873..>2072
                     /locus_tag="ACZPD4_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379157.1"
                     /translation="MREMEFTIEDTEALKPGMEVGVTEGVLPTSYYYTIEHALGMSAN
                     FKQAERLKTRRGIVKELKETPQ"
BASE COUNT          683 a          314 c          495 g          580 t
ORIGIN      
        1 aataggattt tattgacttc tccctttttt acaaacatgc ccaaccctct tttcttgtat
       61 atgatttcct cgtctactaa aagattcatt ccttttaaga cagtatgagg attaatattc
      121 aataaagagg agagttcatt tgtcgaaggg atttttgttt cttcaggaaa cacttttgta
      181 aaaatggagt cttcaagctg ctcagctatt tgaataaaaa taggtttttc tatattagga
      241 ttgatatgca tcgtatcacc accttgtgca ttagtttgtt agttgagtaa ctaactagct
      301 ttgagtatag tatatgtacg gaagaatgtt ttgtcaatac tcaaaaaata aaatatttga
      361 attactattt acaaaataca aacaaaatga tatcataatg atatcaaaca agaacaggag
      421 ggataatgat gaaagaaaag tactgaataa taagaaaaac ggaatgcttg tcttgattct
      481 aacaacattg ttgtatctat tgtcaatcgc agtctgcgtt gtgggagcta tgatcggaaa
      541 tccgttgctg ctcgggatca gtattttttg gatgtgtgta ggctggtttc cgtattgtgg
      601 actgcgtgtg ctgaaacctc aagaagcgct tgtgttgaca ttgttcggaa aatatacagg
      661 cacattgaaa ggagaaggat tttatgcggt aaatccgttt tgtacaagtg taaatccggc
      721 ggcggataca catctaaatc aaagtggtga tgttgacaac agtacaagaa aatcttcact
      781 ttcaggatta ttggcaggca cttcggaaaa atccggtttg gaatcggcag gaaagaagat
      841 ttctttaaag attatgactt tgaacaacag ccgtcaaaag attaacgatt gtctcggaaa
      901 tccggtagaa atcggtattg cggtgatgtg gcgagttgta gatacgtcaa aagcggtttt
      961 taacgtagat aattataaag aatatctttc tcttcagtgt gatacagctc ttaggaatat
     1021 tgtacgtgtt tatccgtatg atgtttctcc gaatgtagat acaaccggcg atggtgtagc
     1081 agacgaggga agtctgagag gctccagcga ggtagtggct gcaagaattc gtgatgagat
     1141 tcaaaaaaga gtaagtgagg caggtctgga aattctggaa gcaagaatca catatcttgc
     1201 ctatgcgccg gagattgcgg cagtgatgct tcaaagacag caggcatcgg ctattataga
     1261 cgcaagaaaa atgattgtag atggagcggt cggaatggta gaaatggcgc ttgatcgtct
     1321 cagcgaaaag aaagttgtag aactggatga agaaagaaaa gctgcaatgg tttcaaatct
     1381 tcttgtcgtc ttgtgcggca acaaagatgc gcagcctatt gtaaattcgg gaagtttgta
     1441 ttgatcatgg cagatcagaa aaagaaacag attccgcttc ggctttctgc aaaattgtat
     1501 gatgccgtgg cagcttgggc agaagatgat tttcgctcag tcaacgggca gattgaatat
     1561 ttgctgaccg agtgtgtaag gcagagaaag aaaaacggaa aatatgtatc ggaacattta
     1621 gatgaacatc ctgaatttga tataaaataa gaagaatgat aaaaaagaaa ctgtattgca
     1681 aatagatatg aaattcgttt gcagtacagt ttttttcaac agtggtcgtt aaagtctaag
     1741 actgccgaag aagagctttc aaaagttttg ttttatgata tactgataaa cgaaaagaaa
     1801 aacagcagcg cagcgaatgg aagctgtcat atttgaatcg cacagtcata gaagagagga
     1861 gtggagaaaa gtatgcgcga aatggagttt acgattgaag acacagaagc tttaaagccg
     1921 gggatggaag tgggagtgac agaaggagtc cttccgacgt cttattacta cacgatcgag
     1981 cacgcactcg ggatgagcgc taatttcaaa caggcagagc ggttaaaaac cagaagagga
     2041 attgtgaaag aattaaaaga aacgccgcag tt
//
LOCUS       DBMDXF010000274         2037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_11376_length_2037_cov_1.634298, whole genome shotgun sequence.
ACCESSION   DBMDXF010000274 DBMDXF010000000
VERSION     DBMDXF010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11376_length_2037_cov_1.634298"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="ACZPD4_08645"
     CDS             complement(<1..543)
                     /locus_tag="ACZPD4_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HIR1379158.1"
                     /translation="MKQIYICEDTITGIYSALHDAWKECRDTQAGVELRGRTQRQLFC
                     EYRIVEESEEKALRLERMIKHHLGYNAYWEIYHALLSTDDRKGTVVFEVLQEARKIRQ
                     SEKIMEHLGCPAVADVFSMSRSVSNEAHRYEEFIRFRELENGILFSEITPKAQILTCV
                     ADHFEDRFPLENWIIYDKTHK"
     gene            complement(617..1921)
                     /locus_tag="ACZPD4_08650"
     CDS             complement(617..1921)
                     /locus_tag="ACZPD4_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR1379159.1"
                     /translation="MLKLMQTEMTLGEKLEILSDAAKYDVSCTSGGTERKGDGEGMGN
                     CRKAGICHSFSADGRCISLLKILFTNECIYDCKYCVNRSGNDVVRTSFTPEEVCTLTM
                     EFYRRNYIEGLFLSSGVLKSPNYTMELLYTVLYKLRHEHNFQGYIHVKAIPGADSRLI
                     QMTGYLADRMSVNIELPTAESLRLLAPHKTRKNILAPMRFVQQMSAENQYEIQTYRHV
                     PKFVPAGQSTQMIIGATPESDYQILHVAESLYKKFDLKRVFYSAFIPVNEDKNLPSVK
                     EQRPPLLREHRLYQADWLLRYYHFEAGELLDEENPNFNAYLDPKCFWALRHLEEFPIE
                     INYAAYDMLLRVPGIGYKSASRIVKARRMGMLDFEDLKKMGVVLKRALYFITCKGKMM
                     YPIRMDEDYITRNLLNTKEKLPEGADGMSFRQLSLFDDMGIE"
BASE COUNT          570 a          508 c          313 g          646 t
ORIGIN      
        1 tttatgcgtt ttatcatata tgatccaatt ttccaatgga aacctatcct caaaatgatc
       61 tgcaacacaa gttaaaatct gcgctttcgg agtaatctcc gaaaacaaaa ttccgttttc
      121 aagttctctg aaacgaataa attcttcata tctatgcgct tcattggaca cgctcctgct
      181 catagaaaat acatccgcaa ctgccggaca ccctaaatgt tccattatct tttcactctg
      241 cctgattttt ctggcttcct gcagcacttc aaatactacc gttccttttc tgtcatctgt
      301 cgaaagaagc gcatggtaaa tctcccagta ggcattgtat ccgagatgat gctttatcat
      361 tctttctaat ctcagcgctt tttcttctga ttcttctact attctgtact cacaaaaaag
      421 ctgccgctgt gtcctccccc tcagttcgac acccgcctgc gtatctctgc attctttcca
      481 ggcatcatgc aaagctgaat aaatgccggt tatcgtatct tcacatatat agatctgttt
      541 catcgcttcc cccactctgc cggatattct cttatttttc aaagtaacca aaaatcagca
      601 gctcttattt tacctcctat tctatcccca tatcgtcaaa taacgacaac tgacgaaagc
      661 tcatcccatc tgcgccttcg ggcagttttt cttttgtatt aaggagattt cttgtaatat
      721 aatcttcatc cattcgaatc ggatacatca tttttccttt acatgtaata aaatagagcg
      781 cccgcttcaa aacaactccc attttcttta aatcttcaaa atcaagcatc cccatcctgc
      841 gcgctttcac aatgcgcgat gctgatttat accctattcc gggaactcta agaagcatat
      901 catatgccgc gtaatttatc tcgatcggaa attcttcgag atgccttaac gcccaaaagc
      961 atttcggatc aagatatgca ttaaaattag gattttcttc atccaaaagt tctcctgctt
     1021 caaaatgata gtatctgaga agccagtcag cctgataaag cctgtgctct cttaaaagag
     1081 gaggtcgctg ctctttgaca gacggaagat ttttatcttc gttgaccgga atgaatgcag
     1141 aataaaatac tcgcttaaga tcaaattttt tatacaacga ctctgccaca tgcaaaatct
     1201 gataatcaga ctccggtgta gcgcctatga tcatctgcgt gctctgtcct gccggaacaa
     1261 acttaggcac atgacgataa gtttgaattt catattgatt ttccgctgac atctgctgta
     1321 caaaacgcat tggtgcaagg atattcttcc gcgttttatg cggcgccaaa agacgaaggc
     1381 tttccgcagt aggaagttct atattcacac tcatcctatc tgcaagatac cccgtcatct
     1441 gaataagacg agaatccgct cccggaatcg ctttcacatg aatatatccc tgaaaattgt
     1501 gttcatggcg cagcttgtat aacactgtat acaaaagttc cattgtataa ttcggacttt
     1561 ttaaaacccc tgaacttaaa aataatcctt ctatataatt tcttctataa aactccatcg
     1621 tcaaagtaca tacttcttcc ggagtaaaag atgtacgtac aacatcattt cctgatcggt
     1681 tcacgcaata tttacaatca taaatacatt catttgtaaa gagaatcttc agaagagaaa
     1741 tacaccgccc gtctgccgaa aaactatgac aaattcctgc tttcctgcag tttcccattc
     1801 cttctccgtc tcccttgcgc tctgtgccgc cagatgtgca tgaaacatcg tattttgccg
     1861 catcagacaa tatctctagc ttttctccta aagtcatctc cgtctgcatc agtttcaaca
     1921 ttttccgaaa accttcctct ttcttgttag aacatgtgtt ctattaaaga gtagcacgta
     1981 tgttctgttt tttcaagagt ctattctgca tccgccacaa taactcaagc aagaaac
//
LOCUS       DBMDXF010000275         1973 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_11752_length_1973_cov_1.528846, whole genome shotgun sequence.
ACCESSION   DBMDXF010000275 DBMDXF010000000
VERSION     DBMDXF010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11752_length_1973_cov_1.528846"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>265)
                     /locus_tag="ACZPD4_08655"
     CDS             complement(<1..>265)
                     /locus_tag="ACZPD4_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1379160.1"
                     /translation="GEPVYVVLMILSIVLNYFCGRDIEANADNPKKAKLSLVFALTAN
                     ILILGFFKYYGFLLDTVNSFLTTDIPYRVLPLPIGISFYTFQAI"
     assembly_gap    266..275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(416..901)
                     /locus_tag="ACZPD4_08660"
     CDS             complement(416..901)
                     /locus_tag="ACZPD4_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1379161.1"
                     /translation="MSQWKKITIRVMRYVTLLLIVGFVALLLIYTSGSNRPFEKVEKS
                     VEASLDKENMKRQDGTALKRNFGLNAADYDGVMYYSSEFSISSEEVLLIKAKSETQTA
                     EISSAIEERIKTRMNDFEGYAPESVQLLEDAKKSVRGKYVFFAAAPGAEKYLEIFNNS
                     L"
     gene            complement(898..1656)
                     /locus_tag="ACZPD4_08665"
     CDS             complement(898..1656)
                     /locus_tag="ACZPD4_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1379162.1"
                     /translation="MKKIKQKTKRKIVLAVCVLAAALVIVLIVQGVRGLFYKKADTSA
                     GLEYIHGEEKGDIAAIEKKIELLEKKDGEEGTGERSLKEKFTGAVIVGDSITEGFTEY
                     DILNTSSVVAKIGVHLDELDEQIKQVKKLSPGIIFLSLGMNDVEHTNGDADEFVKQYG
                     AVVDELKKSVPGAHIFVNAIFPVQEKAVKEKPVFAEIENFNEKLKELCDKKRIAFIDS
                     SDLMSDEYYEQDGIHFKASFYPVWAEKMAEVAGL"
     gene            1845..>1973
                     /locus_tag="ACZPD4_08670"
     CDS             1845..>1973
                     /locus_tag="ACZPD4_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HIR1379163.1"
                     /translation="MIAFSLTNIKDFMSHLLLNETFDHFSFIEGEITTFNTFHIDGF"
BASE COUNT          566 a          483 c          307 g          607 t
ORIGIN      
        1 agatcgcctg aaacgtatag aatgagattc cgatcggcag agggagcact ctgtaaggaa
       61 tgtccgtcgt aagaaaggaa ttaactgtat caagcaaaaa gccataatat ttaaaaaatc
      121 caagtatgag aatatttgcc gtaagagcaa acacaagact tagctttgcc tttttcggat
      181 tgtctgcatt tgcctcgata tcccgtccgc aaaaatagtt taatacgatg gataagatca
      241 tgagcacgac atagaccggc tctccnnnnn nnnnntaaaa aaacagactc gccaaaagca
      301 agacgatatt tttcgccccg ttcggaacga gataatacac tgccattacg attggcagaa
      361 acataaacaa aaatgtgata ctgctaaata acatccgcct gttccctctc ccgatttata
      421 aactgttgtt aaatatttct aaatattttt ctgctcccgg cgcagcggca aaaaatacat
      481 atttaccgcg cacgcttttc tttgcatctt ccaataactg caccgattcc ggcgcatacc
      541 cctcaaaatc attcatcctt gttttgatcc gttcttcgat cgcacttgaa atttccgcgg
      601 tctgcgtttc actttttgct ttaataagaa gaacttcttc agatgaaata ctaaattccg
      661 acgaataata cataacgccg tcataatctg ccgcattcag tccaaagtta cgttttaatg
      721 ccgtcccgtc ctgacgcttc atattttcct tatcaagaga tgcttccaca gacttctcca
      781 ctttctcaaa agggcgattg ctgccgcttg tatagatcaa aagaagtgca acgaatccga
      841 cgatcagaag caatgtcaca tagcgcatca cccggatagt tattttcttc cactgactca
      901 taagcctgcc acctccgcca ttttttcagc ccagaccgga tagaaagacg ccttaaaatg
      961 tatgccgtcc tgctcataat attcgtcaga cataagatcc gaactgtcga taaacgctat
     1021 ccgcttttta tcacataact cttttagttt ctcgttgaaa ttttctattt ctgcaaaaac
     1081 aggcttttct ttcactgcct tttcctgcac cggaaaaatt gcgttcacaa aaatatgcgc
     1141 tcccggcacg ctctttttca gttcatccac aactgctccg tattgtttga caaactcgtc
     1201 cgcatcaccg tttgtatgct cgacatcatt cattccgaga gacaaaaata tgatcccggg
     1261 acttaatttt ttcacctgtt tgatctgctc gtccaactca tccaaatgta ctccgatctt
     1321 tgctacaacg ctggatgtgt tcaaaatgtc atattctgta aatccttccg taatagagtc
     1381 tcccacgata accgcacccg taaacttttc tttcagactc cgttccccgg tgccttcttc
     1441 tccgtctttt ttttcaagca gttcgatttt tttctcaatc gccgcaatat ctcctttttc
     1501 ttctccgtga atatattcca gtcctgccga tgtgtccgct ttcttataaa aaagtccccg
     1561 cacgccctgc acgatcagca caatgacaag cgctgccgca agaacacata ccgcaagaac
     1621 gatcttcctt tttgttttct gttttatttt cttcatttat attgtttccc cattataata
     1681 ctcattttat tttacaaata aaaataaccc aagataataa tacttttttt agattaaaaa
     1741 gtcaatgacg gcacatccgt ttcgtggtac aatcagtatg accctgtcat atcaaaaaca
     1801 tggcaggtaa aatgaacaaa tatacaatga taaggagtct ttccatgatc gcattttcat
     1861 tgacaaatat aaaagatttc atgtcgcacc ttctgttaaa tgaaacattt gatcattttt
     1921 catttatcga aggagagatc acaacattca atacatttca cattgacggc ttt
//
LOCUS       DBMDXF010000276         1938 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_11969_length_1938_cov_1.161132, whole genome shotgun sequence.
ACCESSION   DBMDXF010000276 DBMDXF010000000
VERSION     DBMDXF010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11969_length_1938_cov_1.161132"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="ACZPD4_08675"
     CDS             <1..578
                     /locus_tag="ACZPD4_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1379164.1"
                     /translation="TLDIIISVFMLVITSPIMLITGIAIKLYDGGPVFFRQKRCTIGG
                     KVFEIHKFRSMIVDAEKEGEVIPAKEDDERITPVGKFIRKTRIDELPQFIDILKGDMS
                     VVGPRPERVEHVEQYTAEVPEFSYRLKVKGGLTGYAQIYGKYNTTAYDKLKLDLMYIQ
                     NYSILLDLKLIIMTGKILFVKESTEGFEEKK"
     gene            578..1321
                     /locus_tag="ACZPD4_08680"
     CDS             578..1321
                     /locus_tag="ACZPD4_08680"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR1379165.1"
                     /translation="MASIKAYYGRTYRGSKNRFLDFLQKRIDEKKGCFVVTANPEIFM
                     LGKKMPEMNRVLMDKNTVIAADGIGIVKGGEQLGFRFKERIPGVELCEELLKYANEKK
                     CSIYLFGAKREVLDSFVRRIEKEYKNVIISGYTDGYVEDKEKVMRQITELDLDLVFVA
                     LGAPQQELLIHKYFRSDRKGIYIGVGGSFDVLSGKKQRAPSFFIRHNLEWLYRITKEP
                     KRIKRFWNSNVKFLFELRKESHGGKRTHK"
     gene            1356..>1938
                     /locus_tag="ACZPD4_08685"
     CDS             1356..>1938
                     /locus_tag="ACZPD4_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1379166.1"
                     /translation="MIAVKWKKTERDLWNPPISRVRKDLIDRILRRGYSRIVIYENHF
                     GYHNIMMQRPQHMLRNMGDEETLILYNSYYDIDFKDRRRITPIARHVYVLDLYYYRKY
                     LLNALKQIEKKYVMVYSTDTVPVSRIKQYSELGFRIIYEYVDDINEELISRKKIAQIR
                     SRHQYLLRAKNVLTVATADKLYKEAKADYEEAQK"
BASE COUNT          659 a          247 c          484 g          548 t
ORIGIN      
        1 gaacattgga tattattatt tctgttttta tgctggtgat aacatctcca attatgctta
       61 ttaccggaat cgcgatcaaa ttgtatgatg gaggtcctgt ctttttcagg cagaaaagat
      121 gtacgatcgg gggaaaagta tttgaaattc ataaattccg aagtatgatc gtagatgcgg
      181 aaaaagaggg ggaagtcata ccggcaaaag aagatgacga aaggatcacc cctgtcggaa
      241 aatttataag aaagaccaga attgatgaat tgccgcagtt tattgatatt ttaaaaggag
      301 atatgagcgt tgtaggtcct cgtccggaga gggtggagca tgtagagcag tatacggcgg
      361 aagtgccgga attttcttac agactaaaag tcaagggcgg acttacagga tatgctcaga
      421 tttacggaaa atataatacg acagcgtatg ataagctgaa gcttgacctg atgtatatac
      481 agaattattc gattctgctc gatttgaaat tgatcattat gacaggaaaa atactttttg
      541 taaaagagag tacggaaggg ttcgaggaaa agaagtgatg gcatctataa aagcatatta
      601 tgggagaaca tatagaggaa gtaaaaatag atttttagat tttttgcaga aaaggattga
      661 tgaaaagaaa ggctgttttg ttgtgacagc caatccggag atttttatgc tcggaaagaa
      721 gatgccggag atgaatcggg ttcttatgga taaaaataca gtgattgctg cagacggtat
      781 cggcattgta aagggtggag agcagttggg attccgtttt aaagaaagga taccgggagt
      841 ggaattgtgt gaggaactgc ttaaatatgc aaatgaaaaa aagtgctcga tttatttgtt
      901 cggagcaaaa agagaggttt tggattcttt tgtcagacgg attgagaaag agtacaagaa
      961 tgtcattatt tccggatata cagatggata tgtagaggat aaagaaaagg taatgcggca
     1021 gataacggag cttgatctgg atttggtgtt tgtcgcatta ggcgctcctc agcaggaact
     1081 tttgattcat aaatatttcc gttctgacag aaaaggaatt tatattggcg tcggtgggag
     1141 ttttgatgtt ttaagcggta agaaacagcg tgctccttca ttttttatac gtcataattt
     1201 ggagtggctg tataggatta cgaaagaacc gaagcgtata aaacgttttt ggaacagcaa
     1261 tgtgaagttt ttgtttgaat tgagaaagga aagtcatggc gggaaaagaa ctcataaata
     1321 aaatcagaaa aaagggtata tgcggaacaa tcaagatgat tgccgtaaag tggaagaaaa
     1381 cagagcgtga cttatggaat ccgccgatca gtcgggtgag gaaagatctt atagatcgga
     1441 ttttaagaag aggttattcc aggattgtta tttatgagaa tcactttgga tatcacaaca
     1501 taatgatgca gcgtcctcag catatgctta gaaatatggg ggatgaggag acgctcattt
     1561 tatacaattc gtattatgat atagatttta aggatagacg gcgtatcacg ccgattgcgc
     1621 gtcatgtata tgtattggat ctgtattatt ataggaagta tttattaaat gcattgaagc
     1681 agatagaaaa gaaatacgtc atggtatatt ctactgatac cgttccggta agcagaataa
     1741 aacaatattc agaacttgga tttcggatta tttatgagta tgtagatgat attaatgagg
     1801 aacttatttc aagaaaaaaa atcgcgcaga tccgatcgag acatcaatat ttgttaagag
     1861 caaaaaatgt gttgactgta gcgactgcgg ataaacttta taaagaagca aaggccgatt
     1921 atgaggaagc tcaaaaaa
//
LOCUS       DBMDXF010000277         1913 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12143_length_1913_cov_1.226269, whole genome shotgun sequence.
ACCESSION   DBMDXF010000277 DBMDXF010000000
VERSION     DBMDXF010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12143_length_1913_cov_1.226269"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="ACZPD4_08690"
     CDS             <1..1146
                     /locus_tag="ACZPD4_08690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1379167.1"
                     /translation="EDFLELKKMMRVKEPILSKKADEVNRQFLLLKRECEEYRVLESV
                     SHLAIKLMNLLAEMERFFEKSSESDKRDQVLELYFKVREFLNIHDILDENYVIYSEYQ
                     QDGRFMVKLFCVNPAVNLQAYLEYGIGTVFFSATLLPVRYYKKLLSIETDDYAIYADS
                     PFPKDSRLLLIGRDVSTRYTQRGPEMYRRIASYIVGAADAKKGNYMVFFPSYRVMEEV
                     HSVFSEKCGKISSAVQSPFMSEEDREVFLKEFDEIREESFVGFCVMGGIFSEGIDLPE
                     DKLIGVIIVGTGLPQVCNEREIVKQYFSGIGSDGFEYAYLYPGMNKVLQSAGRVIRTE
                     KDRGLILLLDERFSTGRYRELFPREWSNARHCTAESLTSYMQEFWEC"
     gene            complement(1143..>1913)
                     /locus_tag="ACZPD4_08695"
     CDS             complement(1143..>1913)
                     /locus_tag="ACZPD4_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1379168.1"
                     /translation="TLERKLDQTLFIRSTKKVQLTPEGEILMRHVEPAMNLIQKGETQ
                     LLDAASTGGQIRIGASDTICRYFLIPYLERFHKAFPGAHIKVINQTSMKCAELLRNGL
                     VDLTVVNFPNNHLGTTTSVMRIKKFRDVFIAGNTFQKLKGETLTFSQLLKYPILMLDK
                     NSTTNEFLHQVFQQHQLDLVPEIELTSNDLLIDLARIGLGIAFVPDYCIFPDSEDVFI
                     LHTKEELPERELAIAYNEHLPLSRASMEFLSYFRDKTC"
BASE COUNT          540 a          296 c          487 g          590 t
ORIGIN      
        1 gaagatttcc ttgaattgaa aaaaatgatg cgtgttaaag aaccgattct ttcaaaaaaa
       61 gcagacgaag taaaccgaca gtttctttta ttaaagagag aatgtgaaga atatagagtg
      121 cttgaaagtg tttctcatct ggcgataaaa ttgatgaatc ttttggcgga gatggaacgt
      181 ttttttgaga aatcttccga atcggataaa cgggatcaag ttttagaatt gtattttaaa
      241 gtaagagagt ttttaaatat tcatgatatt ctggatgaaa attatgttat atatagtgag
      301 tatcagcaag acggcagatt tatggtaaag cttttttgtg taaatccggc agtgaattta
      361 caggcatatt tagaatatgg tatcggaacg gtcttttttt cggcaacgct tcttccggtc
      421 cgttattata aaaagctttt atcgattgaa acagatgatt atgcgatcta tgcggattct
      481 ccgtttccga aagacagtcg gctgcttttg atcggaagag atgtgagtac aagatataca
      541 cagcgtgggc cggagatgta ccggcggatt gcgtcatata ttgtgggtgc agcagatgcg
      601 aaaaagggaa attatatggt gttctttccg tcctatcggg taatggaaga agtgcatagt
      661 gttttttcgg aaaagtgcgg aaaaatttct tctgctgttc agtctccgtt tatgagtgaa
      721 gaagacagag aggtgtttct taaagagttc gatgagataa gggaagaaag ctttgtcggt
      781 ttttgcgtga tgggaggaat tttttctgaa gggatagatt tgccggaaga taagttgatc
      841 ggtgtgatca ttgttggaac aggacttcct caagtatgta atgaacggga gattgtcaaa
      901 cagtatttta gcggaatcgg ttcagacggt tttgaatatg catatctcta tccggggatg
      961 aataaagtgc ttcaatctgc aggaagggtg atcaggacag aaaaagacag aggactgatc
     1021 cttttgctcg atgagcggtt ttccacagga aggtatcggg aattatttcc cagagaatgg
     1081 agtaatgccc gacactgtac tgcggaaagt ttgacgagtt acatgcagga attttgggaa
     1141 tgttagcatg ttttatcacg gaagtaactc aaaaattcca ttgaggcacg tgagagcgga
     1201 aggtgttcgt tgtatgcgat agcaagttcg cgctcgggaa gttcttcttt tgtgtgcagg
     1261 atgaatacgt cttcgctgtc agggaaaata caatagtccg gcacaaaagc aatgcctaat
     1321 cctatgcgtg ccagatcgat cagcagatca ttgctcgtaa gttcgatttc gggaacaaga
     1381 tcgagctgat gttgttgaaa tacctgatga agaaattcgt tggtcgtact gtttttgtcc
     1441 agcattaaga tgggatattt caacagttgg gagaaagtca gagtttctcc ttttagtttt
     1501 tggaaagtat ttccggcgat aaacacgtct ctgaattttt tgatgcgcat aacggaagtt
     1561 gttgttccga ggtgattgtt cggaaagttt acaacagtaa gatctacaag tccgtttcgc
     1621 agaagctcgg cacatttcat ggacgtttgg ttgattactt tgatatgcgc gccgggaaat
     1681 gctttatgaa agcgctctaa gtaaggaatc aggaaatagc ggcatatagt atcgctggca
     1741 ccgatgcgta tttgccctcc ggttgatgcc gcgtcgagaa gttgggtttc acctttttga
     1801 atcagattca tagcgggttc aacatgccgc ataagaattt ctccttcggg agtcagttga
     1861 actttttttg tacttctgat aaaaagtgtc tgatctagtt ttctctctaa tgt
//
LOCUS       DBMDXF010000278         1892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12335_length_1892_cov_1.744835, whole genome shotgun sequence.
ACCESSION   DBMDXF010000278 DBMDXF010000000
VERSION     DBMDXF010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12335_length_1892_cov_1.744835"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="ACZPD4_08700"
     CDS             complement(<1..1076)
                     /locus_tag="ACZPD4_08700"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR1379169.1"
                     /translation="MSNDRYVSPLSERYASREMQYIFSPDKKFRTWRRLWIALAETEK
                     ELGLNITDEQIEELKSHADDINYDVAKEREKIVRHDVMSHVYAYGVQCPKAKGIIHLG
                     ATSCYVGDNTDIIVMSEALKLVRRKLINVIAELAKFAEEQKSQPTLAFTHFQPAQPTT
                     VGKRATLWMQEFEMDLEDLDYVLGSLKLLGSKGTTGTQASFLELFDGDQETIDKIDPM
                     IAEKMGFKACYPVSGQTYSRKVDTRVLNVLAGIAASAHKFSNDIRLLQHLKEIEEPFE
                     KTQIGSSAMAYKRNPMRSERIASLSRYVMIDALNPAITSATQWFERTLDDSANKRLSV
                     PEGFLAIDGILDLCLNVVDGLVVYP"
     gene            complement(1115..1822)
                     /gene="purC"
                     /locus_tag="ACZPD4_08705"
     CDS             complement(1115..1822)
                     /gene="purC"
                     /locus_tag="ACZPD4_08705"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1379170.1"
                     /translation="MKKLEQLYEGKAKKVFATEDPDVVIVDYKDDATAFNGEKKGTIV
                     GKGVINNRMTNYVFQVLEKEGVPTHYVEELSDRETAVKKVEIVPLEVIVRNVAAGSFS
                     KRLGIEEGRKLLAPTLEFSYKDDDLGDPLINDYFAIAIGAATREEIDKITEYTFKVND
                     VLKKFFDEAGIDLIDFKIEFGRYHGDIILADEISPDTCRLWDKETHEKLDKDRFRRDM
                     GNVEDAYQEVFKRIGIK"
BASE COUNT          463 a          483 c          319 g          627 t
ORIGIN      
        1 ggataaacga caagcccgtc aacaacattc agacaaagat caagaatgcc gtcgatcgca
       61 agaaatcctt ccggtacact gagacgttta ttcgccgaat catccaatgt tctttcaaac
      121 cactgtgtag cggatgtaat tgccggattc agtgcatcaa tcatcacata tcttgacagt
      181 gatgcaatac gctcgcttct catcggattt ctcttatatg ccatcgccga agaccctatc
      241 tgtgtctttt caaacggctc ttcgatctct tttaagtgtt ggagaagacg aatgtcattt
      301 gaaaacttat gcgcacttgc ggcgattccc gccagcacat tcagcactct cgtatccact
      361 tttcttgaat atgtctgacc ggaaacaggg taacaagctt taaatcccat cttttccgcg
      421 atcatcggat cgatcttatc gattgtttcc tgatctccat caaaaagctc caaaaaactc
      481 gcctgagttc ctgttgttcc cttcgatccg agaagtttca agctcccgag aacataatcc
      541 aaatcttcca gatccatctc gaactcctgc atccaaagtg tcgctctctt tccgactgtt
      601 gtcggctgag ccggctggaa atgtgtaaac gcaagtgtcg gctgactttt ctgctcttct
      661 gcaaattttg ccagctcggc aattacattg atcagttttc tgcgtacaag tttcaacgcc
      721 tcggacatca cgataatatc tgtgttatct ccgacataac aggatgtcgc gccaagatga
      781 atgattcctt ttgctttcgg gcactgtacg ccatatgcat acacatgaga cattacatcg
      841 tgacgtacga tcttctcccg ttcttttgcc acatcgtaat tgatatcatc tgcatgactt
      901 ttcaattctt caatctgctc atccgtaata ttgagtccta attctttttc tgtctccgcc
      961 agtgcgatcc aaagtcttct ccatgtgcgg aacttcttat ccggggagaa aatatactgc
     1021 atttctctgc tcgcatagcg ttcggaaaga ggacttacat aacgatcatt gctcatgtct
     1081 ggttctctcc tttttgtatc atacaatcct tttcttattt gatgccgatc cgtttaaata
     1141 cttcctgata agcatcttct acatttccca tatctctgcg gaaacggtct ttgtcaagtt
     1201 tttcatgtgt ttctttatcc caaagacggc atgtatccgg tgaaatctca tctgcaagaa
     1261 taatatctcc gtgatatctt ccgaactcga tcttaaagtc gatcagatcg attcccgctt
     1321 cgtcaaagaa ctttttcaaa acatcgttta ctttaaatgt atactctgta atcttatcga
     1381 tctcttctct tgtagccgct ccgatggcaa tcgcaaaata atcattgatc agcggatctc
     1441 cgagatcatc gtctttataa ctgaattcca aagtcggtgc gagcagtttt cttccttctt
     1501 cgattcccag tctcttagaa aaacttcctg ctgccacatt acgcacgatt acttcaagcg
     1561 gaacgatctc cacttttttc acggctgtct ctctatcgct gagttcttct acataatgtg
     1621 tcggaactcc ttctttttcc aaaacctgga atacataatt cgtcatacgg ttgttgatca
     1681 cgccttttcc gacgatcgtt ccttttttct ccccgttaaa agctgtcgca tcatctttat
     1741 aatcaacgat cacaacatcc ggatcctctg tggcaaatac tttttttgct tttccttcat
     1801 agagctgttc taattttttc ataacatcca cacctgtcct tttcattaat ttttcaaatc
     1861 tggagtgatt agtatacggc atgtcttatt tt
//
LOCUS       DBMDXF010000279         1887 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12375_length_1887_cov_1.790594, whole genome shotgun sequence.
ACCESSION   DBMDXF010000279 DBMDXF010000000
VERSION     DBMDXF010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12375_length_1887_cov_1.790594"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /gene="gyrB"
                     /locus_tag="ACZPD4_08710"
     CDS             complement(<1..1624)
                     /gene="gyrB"
                     /locus_tag="ACZPD4_08710"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR1379171.1"
                     /translation="MGASSTEFDAEYGADQIQILEGLEAVRKRPGMYIGSTSTRGLHH
                     LVYEIVDNSVDEALAGYCDEIQVNINKDNSVTVCDNGRGIPVGINHKAGLPAVEVVFT
                     VLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVEIYHDGQIYKQRYERGKVVYKL
                     KVVGECEPDRTGTTVTFFPDAEIFDDTVFDYDTLKQRFREMAFLTKGLKIVLRDWRGE
                     ELREHTFHYEGGIKEFVTYLNKSKTPLYEQIIYCEGMKDGVAVEIAMQHNDSYSENTY
                     GFVNNITTPEGGTHIEGFRGALTKTFNDYARKNKLLKDSEPNLSGDDIREGLTAIVSV
                     KIEDPQFEGQTKQKLGNSEARGAVNSILSTQLEIFLEQNPSVAKITVEKSVMAQRARE
                     AARKARDLTRRKSALEGMSLPGKLADCSDKDPVNCEIYIVEGDSAGGSAKTARDRATQ
                     AILPLRGKILNVEKARLDKIYANAEIKAMITAFGTGIHEDFDISKLRYHKIIIMTDAD
                     VDGAHISTLLLTFLYRFMPDLIKEGYVYLAQPP"
     gene            complement(1636..>1887)
                     /locus_tag="ACZPD4_08715"
     CDS             complement(1636..>1887)
                     /locus_tag="ACZPD4_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1379172.1"
                     /translation="RTAALSLKLSEIELVKEELNDTPILLLDDVLSELDKHRQNYLLD
                     SIDNIQTIITCTGLDEFVNHRFSINKIFHIKNGHAVNAN"
BASE COUNT          481 a          458 c          317 g          631 t
ORIGIN      
        1 gaggtggctg tgcaagatat acataacctt ctttgataag atcgggcatg aaacgataaa
       61 gaaacgtcaa cagcaatgtg cttatatgtg ctccgtctac atcggcatcg gtcataataa
      121 tgattttatg atatcttaat tttgaaatat caaagtcttc atgaattccg gttccgaatg
      181 cagttatcat agctttaatc tcggcatttg catatatttt atcgagacgc gccttttcta
      241 cattcaaaat ttttcctcga agaggaagga ttgcctgtgt ggcacgatca cgagccgtct
      301 tcgccgaacc tccggcggaa tctccctcta cgatgtatat ttcacaattt acaggatctt
      361 tgtcggaaca gtctgcaagt tttcccggca aagacattcc ttccaacgcc gattttcttc
      421 tcgtaagatc tcttgctttt cttgcagctt ctcttgcccg ctgagccata actgattttt
      481 caaccgtaat tttggcaacg cttggatttt gttccagaaa aatttcaagc tgagtagaca
      541 gaatactgtt taccgcacct cttgcctcgc tgtttccaag cttttgtttc gtctgacctt
      601 caaattgagg atcttcgatc ttcacgctta cgattgcagt aagcccttct ctgatatcgt
      661 ctccggaaag attcggttcg ctatctttaa gcagtttatt tttccttgca taatcattaa
      721 acgtctttgt aagagcgcct ctgaatccct cgatatgagt tccgccctcc ggcgttgtaa
      781 tattatttac aaaaccataa gtattttcac tgtaagaatc gttatgctgc atcgcaattt
      841 ccactgcaac gccgtctttc atcccttcac agtaaatgat ctgctcgtaa agcggagttt
      901 tacttttatt caaatatgtg acaaactctt tgattccgcc ttcatagtgg aacgtatgct
      961 ctcgtaattc ttcgcctctc cagtctctta atacaatctt cagacctttt gttaaaaacg
     1021 ccatttccct gaaacgctgt ttcaacgtat cataatcaaa caccgtatcg tcaaatattt
     1081 ctgcatccgg gaaaaacgta accgtcgttc ctgtgcgatc aggttcacat tcgccgacca
     1141 cttttaattt atatacaact tttcctcttt cataacgttg cttgtatatc tgaccgtcat
     1201 gatatatttc tacttcaagc cattccgaaa gtgcgtttac tacagatgca cctactccgt
     1261 gaagtcctcc ggatactttg tatcctcctc cgccgaattt tcctcctgca tgaagtacag
     1321 taaatacaac ttcaactgcc ggaagtcccg ctttgtgatt aataccgacc ggaatacctc
     1381 ttccgttatc acacactgta acagaattat ctttgttaat atttacttgt atttcatcgc
     1441 aatatcccgc cagagcttcg tctacggaat tatccacgat ctcatataca agatggtgaa
     1501 gtcctctcgt agaagtgctg ccgatataca taccgggcct tttccttacg gcttccagac
     1561 cttccagtat ctggatctga tccgctccat attccgcgtc aaattctgta cttgatgcac
     1621 ccatgtaata ccctcctaat ttgcatttac tgcatgtccg ttctttatgt gaaatatctt
     1681 gttaatagaa aatctgtgat tgacaaactc atccagccct gtgcatgtta tgatcgtctg
     1741 tatattatcg atactatcca ataaatagtt ttgccgatgt ttgtccagtt cagacaatac
     1801 atcgtcaaga agcaggatag gcgtatcatt taattcttct tttacaagtt caatttcaga
     1861 taatttcagc gataaagccg ctgttct
//
LOCUS       DBMDXF010000280         1867 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12518_length_1867_cov_1.441676, whole genome shotgun sequence.
ACCESSION   DBMDXF010000280 DBMDXF010000000
VERSION     DBMDXF010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_1867_cov_1.441676"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="ACZPD4_08720"
     CDS             <1..947
                     /locus_tag="ACZPD4_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIR1379173.1"
                     /translation="SYDTAKLYKVTDKIDSQLTYKGNIVVKAVDKDYKTVIDELEKDT
                     DYSVEEPAEGNENTLIISLTDAGKIKLKKAINNPDAQAAFLQIRFDTMINKTAKTGEA
                     IYNDADLKFTTAKGTAVDVSVPENERPEVHTGKIAIKKVGEKADGTPLKDVEFMIFDS
                     TEKAQAAVKLLQNNKAGEITGALKVYDPNQKEFTTVVKTNDKGVAEFRGLAYYTNKAN
                     EENKVQGDDAATGAKKYYLVETKTVKGYQLPSKYFEVTVNHDSSTATVPSDTIINTKP
                     SKLPAAGGNGTIIFTAVGLTVMAAAGIIIFMSRKKKDR"
     gene            1024..>1867
                     /locus_tag="ACZPD4_08725"
     CDS             1024..>1867
                     /locus_tag="ACZPD4_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1379174.1"
                     /translation="MSRSKKLIVTALFLIGACILLYPAVSNKLAERHQAEMISRYEQT
                     VEELPKTDMKSEWEKAVKYNESVTGMSVEDPFILGSGSVLPGNYKDVLNVEEKEGVMG
                     YIEIPAIDVMLPIYHGVSERVLRRGVGHMETTALPIGGKGTHSVLASHRGLSTAKLFT
                     DLDRMKEGDLFYIHVLDHLLTYEVDQISVIEPEDTDALRPIADKEYITLLTCTPYGVN
                     SHRLLVRGEKICDEIVKKSEEKESDTAKKERDITEMIAYGAAVFALVSLFVAAVAVCV
                     RKMKK"
BASE COUNT          647 a          287 c          485 g          448 t
ORIGIN      
        1 gcagctatga taccgcaaaa ttatacaaag tgacagataa aatcgattct cagcttacat
       61 ataaagggaa cattgttgtt aaagcggtag ataaggatta taaaacggta atagatgagt
      121 tagaaaaaga tacggattat tcagtggaag agccggcaga aggaaatgag aatacattga
      181 taataagtct gaccgatgcg ggaaaaataa aattgaaaaa agcgattaat aatccggatg
      241 ctcaggctgc atttcttcag atccgttttg atacgatgat caataaaacg gcaaaaacag
      301 gggaagcaat ttataatgac gcagatttga aatttacaac tgcaaagggt acggctgttg
      361 atgtaagcgt gcctgaaaac gagcggccgg aagtacatac cggaaagatt gccattaaaa
      421 aggttgggga aaaggcagac ggaacacctt taaaagatgt tgaatttatg attttcgaca
      481 gtacggaaaa agcacaggca gcagtaaaac ttctccaaaa taataaggcg ggagaaatta
      541 caggagcttt gaaggtatat gatccgaatc aaaaggaatt tactacagtt gtaaagacga
      601 acgataaggg tgttgcagag tttagaggtc ttgcatatta tacaaataag gcgaatgaag
      661 aaaataaagt tcaaggtgat gacgcagcta ccggagcaaa gaagtattat cttgtagaga
      721 caaaaacggt aaaaggatat cagcttccga gtaaatattt tgaagtgaca gtgaatcacg
      781 attcatctac tgctacggtt ccaagcgata cgatcataaa cacaaaaccg agtaaacttc
      841 cggcagcagg tggaaatgga acgatcattt ttacggcagt cggtctgacg gttatggcag
      901 cggcgggcat tattattttt atgtccagaa agaaaaaaga tcgctgatca atagaaagca
      961 gattgaaaga ctaagaagga agagggaggg agtcttagaa gacgaacctc cctttaggtg
     1021 attatgagca gaagtaaaaa gctaatcgta acggctcttt ttctgatcgg agcatgtata
     1081 ctgttatatc cggcagtcag taacaagctt gcagagcgac accaggcaga gatgatcagt
     1141 cgttatgagc agacggttga agaacttccg aagacagata tgaaatcaga gtgggaaaag
     1201 gctgtaaagt ataatgaatc tgttacggga atgtctgtag aagatccgtt tatattagga
     1261 agcggctctg ttttgcccgg taattataaa gatgtgctca atgtagaaga aaaagaagga
     1321 gtgatgggat atattgaaat tccggcgatc gacgttatgc tcccgatcta tcatggagtc
     1381 agtgagaggg tgttgagacg gggcgtcgga catatggaaa caacggctct tcctataggc
     1441 ggaaaaggta cgcattcggt tcttgcatcc cacagaggat tgtctactgc aaaattgttt
     1501 acagatttgg atcgaatgaa ggaaggggat ttgttttata tccatgtatt ggatcatctt
     1561 cttacttatg aggtagatca gatttcagtg atagagccgg aagatacaga tgcattgcgt
     1621 ccgatagcgg ataaggaata cataacgctc ttaacgtgta cgccgtacgg ggtaaacagt
     1681 catagactgc ttgtccgggg agaaaaaata tgtgatgaaa tcgtgaagaa aagcgaagag
     1741 aaagagtcgg atacggcgaa aaaagagcgg gatataacgg agatgatcgc atatggcgca
     1801 gcggtatttg ccctcgtcag tttattcgtt gctgcagtgg cggtgtgtgt aaggaaaatg
     1861 aagaaaa
//
LOCUS       DBMDXF010000281         1860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12578_length_1860_cov_1.822626, whole genome shotgun sequence.
ACCESSION   DBMDXF010000281 DBMDXF010000000
VERSION     DBMDXF010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12578_length_1860_cov_1.822626"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            118..1656
                     /gene="gpmI"
                     /locus_tag="ACZPD4_08730"
     CDS             118..1656
                     /gene="gpmI"
                     /locus_tag="ACZPD4_08730"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1379175.1"
                     /translation="MSKKPTVLMILDGYGLRADDKGNAVAEGKTPVMDKLMTECPFVK
                     GNASGMAVGLPEGQMGNSEVGHLNMGAGRIVYQDLTKITKSIQDGDFFENKALLAACE
                     NVKKNGSALHMMGLVSDGGVHSHNTHIYGLLELAKKQGIEKVYVHCFLDGRDTPPASG
                     KDYVAELEAKMKEIGVGEVASVSGRYYAMDRDNRWDRVEKAYNALVKGEGVEAASALE
                     GIQASYDNEKTDEFVEPFVVMKDGAPTATVKDGDSMIFFNFRPDRAREITRTFCDDQF
                     DGFERGERVKTTYVCFTEYDVTIENKLVAFVKEEITNTFGEFLAAKGLKQARIAETEK
                     YAHVTFFFNGGVEEPNEGEDRILVKSPKVATYDLKPEMSAYEVSDKLVDAIKSDKYDV
                     IIINYANPDMVGHTGVEPAAIKAIEAVDECVGRAVEAVKEVNGQMFICADHGNAEQLI
                     DEETGEPFTAHTTNPVPFILVNADPSYKLREGGCLADIAPTLIELMGMEQPKEMTGKS
                     LLLK"
BASE COUNT          626 a          286 c          497 g          451 t
ORIGIN      
        1 cggacaggtg cacaaatgcg tcagtccggt ctgttcatgc ttgcaaaaca aagaaaaaaa
       61 aggtacaata aatagcggaa tgaaaagtac aaatgtatag ttaaaggaga tatggatatg
      121 agtaaaaaac caaccgtatt gatgatatta gatggatatg gcttgagagc cgatgataag
      181 ggtaatgctg tagcagaagg aaaaactccg gtaatggaca agctgatgac agaatgccca
      241 ttcgtaaaag gaaatgcaag cggaatggca gtcggacttc cggaaggaca gatgggaaat
      301 tcagaagtcg gacatttgaa tatgggagct ggacgaattg tatatcaaga cttgacaaaa
      361 atcacaaagt caattcagga tggagatttc tttgagaata aagcgcttct tgcagcatgt
      421 gaaaatgtaa agaaaaacgg ttctgctctt cacatgatgg gacttgtatc tgacggcgga
      481 gttcacagtc acaatactca tatttacggt ttgcttgagc tggcgaaaaa acagggaatc
      541 gagaaagttt atgtacattg tttccttgat ggaagagata cgcctccggc atcgggaaaa
      601 gattatgtgg cagaactgga agctaagatg aaggaaatcg gtgtcggtga agtggcatct
      661 gtatcaggtc gttattatgc gatggatcgt gacaaccgtt gggatcgtgt ggaaaaagca
      721 tataatgcct tggtgaaagg cgaaggagtt gaagcggcat cggcgcttga aggaattcag
      781 gcatcttatg acaacgagaa aactgacgag tttgtggaac cgtttgtagt gatgaaagac
      841 ggcgctccga cagcgacagt aaaagacggg gattctatga tcttcttcaa tttcagaccg
      901 gatcgcgcga gagagatcac aagaacattc tgcgacgatc agttcgacgg ttttgaacga
      961 ggagaacggg tgaaaacaac gtatgtatgt tttacagaat acgatgttac gatcgaaaat
     1021 aaactggttg cttttgtaaa agaagagatt acaaacacat ttggcgagtt tcttgcagca
     1081 aagggtctga aacaggctcg tattgcagag acggaaaaat atgcgcatgt gacctttttc
     1141 ttcaacggcg gggtagaaga gccgaatgaa ggagaagaca gaatacttgt aaaatctccg
     1201 aaagttgcaa catatgattt gaaaccggag atgagtgcgt atgaagtaag cgataagctt
     1261 gttgatgcaa tcaagtctga taaatatgac gttattatta taaattatgc aaatccggat
     1321 atggttggac atacaggtgt tgagccggca gctatcaaag cgatagaagc tgttgacgaa
     1381 tgcgtcggaa gagcggttga agctgtgaaa gaagtgaatg gtcagatgtt tatctgcgcg
     1441 gatcacggaa atgcagagca gttgattgac gaagagacag gagagccgtt tacggcacac
     1501 acaacaaatc ctgtaccgtt tattcttgta aatgcagatc cgtcttataa gctcagagag
     1561 ggaggatgtc ttgcggatat tgctcctaca ttgattgaac tcatgggaat ggagcagccg
     1621 aaagagatga cgggaaaatc tcttcttttg aaataaaaat ttagcgaatt cgttttatat
     1681 acgaacagac ctcgaagatt cggggtcttt cgtttatgta gagcaataat ggaactttat
     1741 atataaagca gccgtaaagg agtaataaaa ttatgcggga aaagctgaca aagagcgatg
     1801 tagagaaaat tcgggaagag attgaacatc ggaaattggt agaaagaaaa gaattgattg
//
LOCUS       DBMDXF010000282         1858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12587_length_1858_cov_1.598179, whole genome shotgun sequence.
ACCESSION   DBMDXF010000282 DBMDXF010000000
VERSION     DBMDXF010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12587_length_1858_cov_1.598179"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="ACZPD4_08735"
     CDS             complement(<1..206)
                     /locus_tag="ACZPD4_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1379176.1"
                     /translation="MTLPLSAPMLDLLVLSVIAEEDAYGYQIGRIIKRASATMKDSAL
                     YPILKRLQENHFVETYDRQFQGRN"
     gene            complement(379..>1858)
                     /gene="clpX"
                     /locus_tag="ACZPD4_08740"
     CDS             complement(379..>1858)
                     /gene="clpX"
                     /locus_tag="ACZPD4_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1379177.1"
                     /translation="FILVDEEEKESTGADEVDKEEKKDHLAVKKEEEKETEKDNDDDG
                     YEKICLICHRPESVAGKMIEMPNHITVCRDCMQRSFDAMTDGSVDLSRLVNMPGVQFL
                     NLSDLDHMQPKAQKIKKKKEKPKEFHQLDIKKIPAPHKIKARLDEYVVGQEKAKKAMA
                     VAVYNHYKRVASDTMDDIEIEKSNMLMIGPTGSGKTYLVKTLAKLLDVPLAITDATSL
                     TEAGYIGDDIESVVSKLLAAADNDVERAEQGIIFIDEIDKIAKKHTSNQRDVSGESVQ
                     QGMLKLLEGSEVEVPVGANSKNAMVPLTTVNTKNILFICAGAFPDLEDIIKERLTKKT
                     SMGFNAELKDKYEHDEDILSKVTVEDLRKFGMIPEFLGRMPIIFTLKGLDKDMLVKIL
                     KEPRNAILKQYQKLLALDEVSLVFDDGALETIAEKAMEKDTGARALRAIIEEFMLDIM
                     YEIPKDDNIGEVTITREYIEGTGGPVIKLRGQQSIAMKEDHS"
BASE COUNT          449 a          474 c          303 g          632 t
ORIGIN      
        1 ctgtttcttc cctgaaactg tctatcatac gtttccacaa aatgattttc ctgaagccgc
       61 tttaatatcg ggtacaacgc agaatctttc atcgtagccg acgctctttt aatgatcctg
      121 ccgatctgat aaccatacgc atcttcttct gcaatcacag agaggacaag aagatcgagc
      181 atcggtgcac tcaacggtaa tgtcatattc tcactccttt atatatgttt tatttattta
      241 tatgtttaat atattcatat tatataatat ataatatgaa tggtcaatat atatgtttga
      301 aagtttttct aaagaaaatc aaaagaaaag ccggaaggct ctgatctatg accaaatctt
      361 ccggttttac tctctctttt acgaatgatc ctctttcatc gcgatcgact gctgtccccg
      421 cagtttaatg acaggacctc ccgtaccttc aatatactct cttgtgatcg tcacttcccc
      481 gatgttgtca tctttgggga tttcatacat aatatccaac ataaactctt cgatgatcgc
      541 ccgaagcgct ctcgccccgg tatctttttc cattgctttt tccgcaatcg tctcaagagc
      601 gccgtcatca aacaccagac ttacttcatc aagggcaagc agcttctgat actgttttaa
      661 gatcgcattt ctcggctctt tcaagatctt aacaagcata tctttatcaa gacctttcaa
      721 tgtaaatatg atcggcatac gtccgaggaa ctccgggatc ataccgaatt ttctcagatc
      781 ttccaccgtc actttcgaca aaatgtcctc gtcatgctca tacttatctt ttagttctgc
      841 attaaatccc atagacgttt tctttgtaag acgctctttt ataatatctt ccagatccgg
      901 aaatgcgccg gcacaaataa acaaaatatt tttcgtattg accgttgtca aaggaaccat
      961 cgcgttcttg ctgttcgctc cgacaggaac ttccacttcg cttccttcca aaagtttgag
     1021 cattccctgc tgaactgatt ccccgcttac atctctttga ttcgacgtat gtttttttgc
     1081 gatcttatcg atttcatcga taaaaataat tccctgctcc gcccgttcta catcgttgtc
     1141 ggcagcggca agtagtttgg aaacaacgct ttctatatca tctccgatat atcctgcttc
     1201 tgtcagcgac gtagcgtctg taatggcgag cggcacgtca agaagttttg ccaatgtttt
     1261 gacaagatac gtctttccgc ttcccgtcgg tccgatcata agcatattcg acttttcaat
     1321 ctcgatatcg tccattgtat ctgacgcaac ccgtttataa tgattataaa cggcgactgc
     1381 catcgctttc ttcgcttttt cctgtccgac tacatattcg tcaagtcttg ctttgatctt
     1441 atgaggcgcc ggaatcttct taatatcaag ttgatggaac tctttcggct tttctttttt
     1501 cttcttgatc ttctgcgctt taggctgcat atgatccaaa tccgataaat taagaaactg
     1561 tacccccggc atgttgacaa gccggctcag atcaacagag ccgtctgtca tcgcatcaaa
     1621 gcttctttgc atacaatccc ggcataccgt aatatgattc ggcatctcga tcatctttcc
     1681 cgcaacgctt tccggtctgt gacagatgag acagatcttc tcatatccat catcatcgtt
     1741 gtctttttcc gtttcttttt cctcttcctt ctttactgcc aaatggtctt ttttctcttc
     1801 tttgtccacc tcgtctgccc ctgtggactc tttttcttcc tcatctacta aaatgaaa
//
LOCUS       DBMDXF010000283         1846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12679_length_1846_cov_2.241834, whole genome shotgun sequence.
ACCESSION   DBMDXF010000283 DBMDXF010000000
VERSION     DBMDXF010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_1846_cov_2.241834"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="ACZPD4_08745"
     CDS             <1..545
                     /locus_tag="ACZPD4_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1379178.1"
                     /translation="AKRILQKGGYEELSIKNICEEAGVSNGSFYHHFKTKDDLLSYYI
                     EDQPQIEPNLLELPDSVQAVKDGIIQVYLNYVKYCRELGVEFMSGYYDTKNQALNADI
                     RTERPYPIVTVRAYVEKAVQAGVAVLNEPIEEFTTDIRMIVIGNVFEWCIKHGKADFE
                     GNMSRSLRCYLDGALREAVR"
     gene            620..1546
                     /locus_tag="ACZPD4_08750"
     CDS             620..1546
                     /locus_tag="ACZPD4_08750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1379179.1"
                     /translation="MEKEYDFIINPKARSGMGMKAWQKIESELKARHINYRVYLTKRR
                     GHAAKTAAFITADDKRHTIVVLGGDGTVNEVVNGIKRPDLITFGYIPIGSSNDFARGL
                     KLPKDPMKALQSVLSPKKVISADVGQISREGKSRRFIVSAGMGFDAGVCHEVCVSQWK
                     KRLNKIGLGKLSYAVVALDRLKKDRPTKLTVTLPDGRKQMFEKTLFVAFMNLPYEGGG
                     FRFAPEASVTDGCIDIVIAHHMSPLKAVWLLPRAFFGKHTGAKEITIIRSPSVSVEAQ
                     RSLPIHTDGEAAFSRYKASVEILEEKLHIIAG"
     gene            1675..>1846
                     /locus_tag="ACZPD4_08755"
     CDS             1675..>1846
                     /locus_tag="ACZPD4_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1379180.1"
                     /translation="MGKKESGKFGSWLKKYKHAWVFLYIFIYMPWFLLLEKHVTTNYH
                     VIQTQFDMWIPFV"
BASE COUNT          586 a          309 c          484 g          467 t
ORIGIN      
        1 cagcgaaacg cattttgcag aagggcggat acgaggaact gtctatcaaa aatatatgtg
       61 aggaggcggg tgtctccaat ggaagctttt accaccattt taagacaaaa gatgatcttc
      121 tttcttacta tatagaagat cagcctcaga tcgaaccgaa tcttttggaa cttccggaca
      181 gcgtacaagc agtaaaagac ggtattatcc aagtgtattt gaattatgta aaatactgcc
      241 gtgaacttgg agttgagttt atgtccggtt actatgatac gaaaaaccag gcgttgaatg
      301 cagatatccg tacagaacgt ccgtatccga tcgtgacagt aagagcttat gtcgaaaagg
      361 cagtgcaggc aggcgtcgca gtattgaatg agccgatcga agaatttacg acagatatca
      421 ggatgatcgt aatcgggaat gtgtttgagt ggtgcattaa gcatggaaag gctgattttg
      481 aaggaaatat gagccggtcg cttagatgtt atctcgacgg ggcgcttcgc gaagcggttc
      541 gctgacgaaa gacaaacaaa acagaacata aaaagcggac ggaacttata aaaggaacaa
      601 tagataagag ataataaaaa tggaaaaaga atatgatttt attataaacc cgaaagcgcg
      661 ttcaggaatg ggaatgaaag catggcagaa aatagaatca gaattgaagg cacgacatat
      721 aaattaccgt gtatatctga caaaaagaag aggtcatgcc gctaagacgg cggcgtttat
      781 cacggcagat gataaacggc atacgattgt tgttttaggc ggagacggca cagttaatga
      841 agtcgtaaac gggataaaac gtccggatct gataacattt gggtatatcc ctatagggtc
      901 gagcaatgat ttcgcgcggg gattaaaact tccgaaagat ccgatgaaag cacttcaatc
      961 tgtattatct ccgaagaaag tgatttctgc cgatgtagga cagatttcaa gagaaggaaa
     1021 aagccggaga tttattgtaa gcgcagggat ggggttcgac gcaggagtct gtcatgaagt
     1081 gtgcgtgtca caatggaaaa agcggctgaa taaaataggg ctgggtaaac taagttacgc
     1141 agtagttgcc cttgaccgtc tgaaaaaaga ccggccgaca aagttgacgg taacgctgcc
     1201 tgacgggaga aaacagatgt ttgaaaaaac tctttttgtg gcttttatga atcttccata
     1261 tgaaggcggc ggttttcgat ttgccccgga ggcgtccgtg acggatggct gcatcgatat
     1321 cgtgatagcg caccatatgt ctcctttgaa ggcggtgtgg ctgttgccga gagcgttttt
     1381 tgggaaacat acgggagcaa aagaaattac gatcattcgg agtccttcgg tatcggttga
     1441 ggcgcagcga agccttccga tccatacaga cggagaggcg gcgttttccc gatataaggc
     1501 atcggtggag atactggaag aaaaattgca tatcattgca ggataacagc cggaaaattt
     1561 aataaatgtt tcctaaatgt ttaatgaaga aggttgttgt acattttaaa tcgtgctata
     1621 atcgttgcaa tcgcaaagat taaaagattg catatgaaaa ggggcagtcg tgtaatgggc
     1681 aaaaaagaaa gtggtaaatt cggaagctgg ctgaaaaagt ataaacatgc gtgggtattt
     1741 ttgtatattt ttatctacat gccgtggttt ttgcttttgg aaaagcatgt aacgacaaac
     1801 tatcatgtga tccagacgca gtttgatatg tggattccgt ttgttg
//
LOCUS       DBMDXF010000284         1839 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12739_length_1839_cov_1.838320, whole genome shotgun sequence.
ACCESSION   DBMDXF010000284 DBMDXF010000000
VERSION     DBMDXF010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12739_length_1839_cov_1.838320"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /gene="obgE"
                     /locus_tag="ACZPD4_08760"
     CDS             complement(<1..1139)
                     /gene="obgE"
                     /locus_tag="ACZPD4_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1379181.1"
                     /translation="MFADRAKIYIRSGKGGDGHCSFRRELYVPNGGPDGGDGGRGGDL
                     IFAIDEGLNTLVDYRHKRKYAAGDGEPGGKRRCHGKDGKDLILYVPEGTVIKEAVTGK
                     VIADMSGDNRRQIVLKGGKGGLGNQHFATSTMQVPKYAQPGQPARELEVKLELKVIAD
                     VGLIGFPNVGKSTLLSRVTNAEPKIANYHFTTLSPNLGVVDLEGAKGFVMADIPGLIE
                     GASEGVGLGHEFLRHIERTKLMIHVVDAAGTEGRDPIDDIYKINAELEAYNPEIAKRP
                     QVIAANKTDLIFEADEDPTERLKKEFEPKGIKVFPISGVTGKGISELLYYVSAQLETM
                     DRKTVVFEQEYFPEDELIYEELPYTVELEDGMYIVEGPKIEKMLG"
     gene            complement(1321..1605)
                     /gene="rpmA"
                     /locus_tag="ACZPD4_08765"
     CDS             complement(1321..1605)
                     /gene="rpmA"
                     /locus_tag="ACZPD4_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1379182.1"
                     /translation="MMKLNLQFFAHKKGVGSTKNGRDSESKRLGAKRADGQFVKAGNI
                     LYRQRGTKIHPGVNVGRGGDDTLFALVDGVVRFERKGRDKKQVSIYPVAE"
     gene            complement(1611..>1839)
                     /locus_tag="ACZPD4_08770"
     CDS             complement(1611..>1839)
                     /locus_tag="ACZPD4_08770"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1379183.1"
                     /translation="AASMLVINTINAIELYTKDETSMVSDDISGIIEYEIKDRPSKEA
                     QLLMKTLVLGLKQMADDENYAEYIDLTFEEV"
BASE COUNT          462 a          461 c          321 g          595 t
ORIGIN      
        1 tatccaagca ttttttcgat tttgggacct tcgactatat acataccgtc ttccagttct
       61 acagtatacg gaagttcctc gtagataagt tcgtcttcag ggaaatattc ctgctcaaaa
      121 acaacagtct tgcgatccat cgtttctaac tgagcgctga cataataaag cagttcggag
      181 ataccttttc ccgtaactcc cgaaatagga aaaactttga tgcccttcgg ctcaaattcc
      241 tttttcaaac gttctgtagg atcttcatca gcctcaaaaa taagatcagt cttatttgcg
      301 gcgatcactt gaggacgttt tgcgatttcg ggattatacg cttccaattc cgcattaatc
      361 ttataaatgt catcaatcgg atctctgcct tctgtgcctg cggcatcaac aacatggatc
      421 atcaattttg ttctttcgat gtgacgtaaa aactcatgac ctaatccgac gccttcagac
      481 gctccttcga tcagtcccgg aatgtctgcc atcacaaaac cttttgcacc ttcaagatct
      541 actacaccca gattcggact taatgttgta aaatggtaat tggcaatttt gggttccgcg
      601 tttgtcacac gggaaagtaa agtagacttt cctacattcg ggaatccgat cagtcccaca
      661 tctgcgatta ctttcaattc aagttttact tccaattccc ttgcaggttg tcccggctgt
      721 gcatatttcg gaacctgcat tgtcgatgtg gcgaaatgtt ggtttccaag tcctcctttt
      781 ccgcctttta atacaatctg tctgcggtta tcgccggaca tatcggcaat aactttccct
      841 gtcacagctt ctttgatcac tgttccctcc ggaacataaa ggataagatc tttcccgtct
      901 tttccgtggc atcgccgttt tccgcccggt tctccgtctc ctgccgcata ttttcgttta
      961 tggcggtaat ctacaagcgt atttaatcct tcatcgattg caaagattaa atctcctccg
     1021 cgtcctccgt ctccgccgtc gggtccgccg ttcggtacat agagttctct tctgaaactg
     1081 caatgtccgt ctccgccctt gccggaacgg atatatattt tcgctctgtc tgcaaacata
     1141 atttctcctt tatctttcct gctttgtata tcaggaagaa tcaaatgata attgttaccg
     1201 aaaaacttca ttaaaagttt atctcatgta tagaaacaaa agccccagac aaaaagtctg
     1261 gggcttatac tgcctgttat acgagtgaaa agttccggca agacttttcg tcaagtccga
     1321 ttattcagct accggataga tagaaacctg tttcttatct cttccttttc tttcaaatct
     1381 taccacaccg tcaacaagtg cgaataatgt atcatctccg ccacgtccta cgttgacacc
     1441 tgggtgaatc tttgttccgc gctgtctgta aaggatgtta ccagctttta caaactgtcc
     1501 gtctgctctc ttggcaccaa gtctcttaga ctcagaatct ctaccgttct tagtagaacc
     1561 aactcctttt ttatgagcga aaaactgaag gtttaatttc atcatggtag ttacacctcc
     1621 tcgaatgtta aatcaatata ttctgcatag ttctcatcat ccgccatctg ttttaaacca
     1681 agaaccaatg tcttcataag aagctgcgct tcctttgaag gcctgtcttt gatctcatat
     1741 tcaatgattc ctgagatatc atctgaaacc atagacgtct catcttttgt atagagttca
     1801 atagcattaa tcgtattgat taccagcata gatgctgcc
//
LOCUS       DBMDXF010000285         1833 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_12799_length_1833_cov_1.789261, whole genome shotgun sequence.
ACCESSION   DBMDXF010000285 DBMDXF010000000
VERSION     DBMDXF010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12799_length_1833_cov_1.789261"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..790)
                     /locus_tag="ACZPD4_08775"
     CDS             complement(68..790)
                     /locus_tag="ACZPD4_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379184.1"
                     /translation="MGIYEFAGCVVSYEPLYGLLRRRMETYRSDTEGQPDITLDGTKE
                     DYEKLRKQYPHLSIEECEYSCAGAEFYMKLLKHQGFMIHASAVEKEGKAYLFSAPSGT
                     GKSTHAQLWRQTFKGEPIRIINDDKPAVCLQNGVFYACGTPFSGKTDKNENRKAPIQG
                     LCMLHRGKTNKIQKITSGEALPLILPQTLFYGDEEETNLLLSFLDRFLQSVPVYKMEC
                     TMEEEAAKTAYFLGNCETSPQS"
     gene            complement(846..>1833)
                     /locus_tag="ACZPD4_08780"
     CDS             complement(846..>1833)
                     /locus_tag="ACZPD4_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379185.1"
                     /translation="RNKKIQILLVFVLAIGLFGLLFYWRYGEKKQMESLNGLQETYKE
                     YDKKIREIRNDMENKKAEVNDIEKPANVILAFSEEDQELMDRIVPALEGRGIQATLVL
                     KNTAEHHQETVTYLGQQGWDFAFGGEIGEEKDAYIEMLKSTVKQYEESTGKIAGAYFF
                     NGKEYGRGSKILYPHFTEMDFKIGVAFAKDASSLKHGKNTDYNMEIEECQNISMREDM
                     ETIENILDQVIEARSVVVLSDFSLERQMEIAGEASLEHFEEILDLLLQKQSESDLVVG
                     SVTCYEGTLEDRANRIEQRRQKYSEYEQKCLEEIEQLTKQRDEALEKQEVKK"
BASE COUNT          458 a          487 c          281 g          607 t
ORIGIN      
        1 agaaatgaac gattttcact tcctatccca agaatcctgc atgacaggag ccttcgcctg
       61 ttttcagtca ggattgcgga cttgtttcgc aattacctaa aaaatatgcc gtcttagccg
      121 cttcttcttc catagtacac tccattttgt aaaccggaac agactgtaaa aaacgatcta
      181 aaaaagagag cagcaaattc gtctcttctt catctccgta aaataatgtc tgcggcaata
      241 tcagaggaag agcttccccc gaagtaatct tctgtatttt atttgttttt cctctatgca
      301 gcatacaaag tccttgtatc ggcgctttgc gattctcatt tttatcggtt ttcccgctga
      361 acggagtccc gcatgcataa aatacgccgt tttgcagaca aactgcgggc ttgtcatcgt
      421 taatgatccg aatcggttct cctttgaacg tctgccgcca aagctgcgca tgcgtagatt
      481 tccctgttcc gctcggagcc gaaaaaagat aggcttttcc ctctttttct actgccgaag
      541 catggatcat aaacccttga tgttttaaca gtttcatata aaattcagct ccggcacaac
      601 tatattcaca ctcttctata gacaggtgcg gatattgctt tctcagtttt tcataatctt
      661 ctttcgtacc gtcaagcgtt atatcaggct gcccctctgt atccgaccga tacgtctcca
      721 tacgccgccg aagcagaccg taaagcggtt cataagatac aacacatcct gcaaattcat
      781 aaatccccac tgtacttcct cccgaatcac aaatctaatc tgtccttttt ccgagaaaac
      841 tccctctatt ttttcacttc ttgtttttca agcgcttcat cacgctgttt tgttaactgt
      901 tcgatttctt ccagacactt ctgttcgtat tccgaatatt tctgacgacg ctgttctatt
      961 ctgttcgctc tgtcttctaa agttccttca taacatgtaa cgcttccgac tacaagatca
     1021 ctttcacttt gtttttgtaa gagcagatca agtatttcct caaaatgctc cagcgaagcc
     1081 tcgcctgcaa tctccatctg ccgttccaaa ctaaaatccg ataaaacaac tacgcttctc
     1141 gcttctatga cctgatccaa aatattttcg atcgtttcca tatcttcacg catggaaata
     1201 ttctggcatt cttctatctc catattgtaa tctgtatttt ttccatgctt caaagaagat
     1261 gcatctttcg caaaagccac cccgatctta aaatccattt ctgtaaaatg cggatataaa
     1321 atcttgcttc ctcttccata ttccttaccg ttaaaaaaat aggcgcccgc aatctttccc
     1381 gtactttctt cgtattgttt gacagtactt ttcagcattt ctatataagc gtctttctcc
     1441 tccccgatct cacctccaaa agcgaaatcc cagccttgct gtcctaaata cgtcactgtt
     1501 tcctgatgat gctccgctgt gtttttcaaa acaagagtgg cctgtatccc tctgccttca
     1561 agagccggaa cgatccgatc catcagttcc tgatcttcct ctgaaaatgc aagaattacg
     1621 ttcgccggtt tctcaatatc attcacttct gcttttttat tttccatgtc attgcgtatc
     1681 tctcggatct ttttatcata ctccttgtac gtttcctgca acccgtttaa gctctccatc
     1741 tgcttttttt ccccgtatct ccaatagaaa agcagaccga aaagtccgat tgccaaaaca
     1801 aatacgagca aaatctgtat tttcttattt ctc
//
LOCUS       DBMDXF010000286         1807 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13013_length_1807_cov_2.126612, whole genome shotgun sequence.
ACCESSION   DBMDXF010000286 DBMDXF010000000
VERSION     DBMDXF010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13013_length_1807_cov_2.126612"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="ACZPD4_08785"
     CDS             complement(<1..1251)
                     /locus_tag="ACZPD4_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1379186.1"
                     /translation="MDLFDYMRETKKEKESPLASRMRPKTLDEVVGQQHIIGKDKLLY
                     RAIKADKLSSVIFYGPPGTGKTTLAKVIANTTSAEFKQINATVAGKKDMEEVVKEAKD
                     MQGMYGKRTILFIDEIHRFNKGQQDYLLPFVEDGTVILIGATTENPYFEVNGALLSRS
                     AVFELKPLEKEDIKILLRRAVEDEEKGLGSFGAKIEEEALEFLADMSGGDARSALNAI
                     ELGVLTTERSEDGIIHITLETASECIQKRVVNYDKKGDNHYDIISAFIKSMRGSDPDA
                     AVFYLAKMLYAGEDVKFIARRIMICAAEDVGNADPMALTVAVSAAQAVERIGMPESQI
                     ILSQAVTYVACAPKSNSAVNAIFAANEAVRNYQTSVPAHLRDAHYKGAKKLGHGTDYK
                     YAHDYPDHYVKQQYLPDEIKNARFY"
     gene            complement(1294..>1807)
                     /locus_tag="ACZPD4_08790"
     CDS             complement(1294..>1807)
                     /locus_tag="ACZPD4_08790"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1379187.1"
                     /translation="TIGRMKTDVMRERIKDIDPNIKVETYETFVLPDNAKELLEQIGT
                     IHYIIDAIDTVSAKIVLAELAGERNIPIISSMGTGNKLHAEMFEITDLSKTSVCPLCK
                     VMRKELRNRGITHLKVLYSKEKPISAEGRTTEEEKGMRRTIPGSVSFVPPVAGLLIAG
                     EVIRSLIQAE"
BASE COUNT          435 a          487 c          299 g          586 t
ORIGIN      
        1 atagaatcgg gcatttttta tttcatcagg aaggtactgc tgtttcacat aatgatccgg
       61 ataatcatga gcgtacttat aatctgtgcc atgaccgagt ttttttgccc ctttataatg
      121 cgcgtctcta agatgtgccg gcacgcttgt ctgatagttc ctcaccgctt catttgccgc
      181 aaaaatcgca ttgaccgcag agttactttt aggcgcacat gctacatacg tcactgcctg
      241 agataaaata atctgagact ccggcatgcc gatccgctct accgcctgtg ccgccgaaac
      301 ggcaaccgtc agagccatcg gatctgcatt tcccacatct tccgctgcgc atatcataat
      361 gcggcgggca ataaacttga catcttctcc cgcatacagc atttttgcca aataaaacac
      421 tgccgcatcc ggatctgatc cacgcatact tttgataaac gccgaaataa tatcgtaatg
      481 attatcccct tttttatcat agtttaccac tcttttctgt atacattccg aagctgtttc
      541 aagagtaata tgaataatcc cgtcttctga ccgttctgtc gttaaaacgc ccagttcaat
      601 cgcattcaac gcgcttctgg catctcctcc cgacatatcc gctaaaaatt caagcgcttc
      661 ttcttctatc ttggcgccaa aacttccgag acctttttct tcatcttcca ccgcacgtct
      721 aagtaatatt ttaatatctt ctttctcaag cggtttcaat tcaaacacgg cagaacggga
      781 aagcaaagct ccgtttacct caaaatatgg attttctgtt gtagccccga tcaatatcac
      841 tgttccgtcc tccacaaacg gaaggagata atcttgctgg cctttattaa acctatgaat
      901 ctcatcgata aaaagaatcg tccgtttccc gtacattccc tgcatatctt tcgcttcttt
      961 tacgacttcc tccatatcct ttttccccgc aacagtcgca ttgatctgct taaattctgc
     1021 acttgtcgta tttgcgatca cttttgcaag agttgtttta cctgttcccg gaggaccgta
     1081 aaaaataacg gaactaagct tatccgcctt gatcgcccga tataacaatt tatcctttcc
     1141 tataatatgc tgttggccga ccacttcatc caaagtctta gggcgcattc tggatgccag
     1201 cggcgattct ttttccttct ttgtctccct catataatca aataaatcca tctggttctt
     1261 cctttcgtca ctcttcttgt tttctctgta aatctactct gcctgtatca aactgcggat
     1321 tacttctcct gcgatcagca agcccgcaac aggcggaaca aacgaaacac ttcccggaat
     1381 ggttcttcgc attcccttct cttcttctgt cgtacgcccc tctgcgctga tcggcttttc
     1441 ctttgaatac aacactttca aatgtgtgat gcctcggttt ctaagttcct ttctcatcac
     1501 tttacaaaga ggacatacac ttgtttttga cagatctgta atctcaaaca tttctgcatg
     1561 aagcttattt cctgtaccca tgctgcttat aatcggaata ttccgttccc cggcaagttc
     1621 cgcaaggaca attttcgcac tgaccgtatc gatcgcatct attatataat gaatagttcc
     1681 gatctgttcc agaagttctt ttgcattgtc cggcagaaca aaagtctcgt atgtttccac
     1741 tttgatattc ggatcgatgt ctttgattct ctctctcatc acatccgttt tcattcttcc
     1801 gatcgta
//
LOCUS       DBMDXF010000287         1786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13187_length_1786_cov_2.368546, whole genome shotgun sequence.
ACCESSION   DBMDXF010000287 DBMDXF010000000
VERSION     DBMDXF010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13187_length_1786_cov_2.368546"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="ACZPD4_08795"
     CDS             complement(<1..137)
                     /locus_tag="ACZPD4_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845891.1"
                     /note="EngC; RsgA; CpgA; circularly permuted GTPase;
                     ribosome small subunit-dependent GTPase A; has the pattern
                     G4-G1-G3 as opposed to other GTPases; interacts strongly
                     with 30S ribosome which stimulates GTPase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1379188.1"
                     /translation="MQGKIVKGIAGFYYVHVVESGVYECKAKGIFRKDGVKPLVGDNV
                     E"
     gene            complement(165..>1786)
                     /locus_tag="ACZPD4_08800"
     CDS             complement(165..>1786)
                     /locus_tag="ACZPD4_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR1379189.1"
                     /translation="GKVKVTDFGTARATTSTQTISTSVMGSVHYTSPEQARGGVVDQK
                     SDLYSIGITMYEMITGHVPFDGDSTVSVALKHLQEEITSPAEEVPNIPYSLECIIMKC
                     TQKNPGRRYPDCASLLMDLKRSLVEPDGDFVVLGAGTGAETDRTMVMSTEELERIQGS
                     RYDDEYDDDDEYDDDDDYDYDDDNDYDNRRRSQKDRRKKNDVNPDTKRIMKILMIVSG
                     VVIALLILFLVGNAAGFFKGAGLSSKTEKMVTVPDVRGMTFEDAQAELKKKDLGIKKA
                     DKEEASNEYEKGEIKSQNPGKGKKVKKNSTVTVVISSGEAAKKVTVPNVVNRSEAEAE
                     RMLQDAGLKVTKGEAVYDDTIEMGNVVSSTPGSGEQVDEGTSVQLIISKGKDPEKDKV
                     EVPSLLNMTADQAAAALEELGLKGSPKEEYAGGTAGTVVTQETEAGTKVKKGTTIKYT
                     VTLGSKTEWSTIPSDVIGMEKNTAVKVLTEAGFVPVYGGEDYSDTPVGTIYSVTPSAG
                     TKVEKGTQVYYFVSKGSSSGQTDPENGGDNE"
BASE COUNT          374 a          520 c          317 g          575 t
ORIGIN      
        1 atctctacat tatctcctac aagaggcttc acaccgtctt ttcggaagat cccttttgct
       61 ttacattcat aaacaccgga ttctactaca tgaacgtagt agaatccggc aatccctttt
      121 acaattttac cttgcatatt ttataaattc cctgtcggta ccgcctattc attgtctccg
      181 ccgttttccg gatcggtctg tcctgacgat gatcctttgc tgacaaaata atatacttgt
      241 gtcccttttt ccacttttgt acctgccgac ggagtcacac tgtaaatcgt tccgaccgga
      301 gtatcactgt agtcttcacc gccgtatacc ggaacaaacc ctgcctcagt cagcacttta
      361 accgccgtgt ttttctccat tccgatcaca tcactcggaa tagtagacca ttctgttttt
      421 gatccaagcg ttaccgtata ctttattgtt gtgcctttct ttactttagt tcccgcttct
      481 gtttcctgtg tgacaaccgt tcctgccgtt ccgccggcat attcttcctt aggacttcct
      541 ttcagtccaa gttcttctaa ggcggcggca gcctgatccg ccgtcatatt cagaagcgac
      601 ggcacttcta ctttatcttt ttcgggatct tttcctttac tgataataag ctgaacgctt
      661 gtgccctcgt ctacctgctc tcccgaaccc ggagtagagc tgactacatt tcccatctcg
      721 atcgtatcat cataaactgc ctcgcctttt gtcactttca gaccagcatc ctgaagcatt
      781 ctctctgctt ccgcctccga tctgttcaca acattcggca cagttacttt tttcgcagct
      841 tctccgctgc tgatcacaac cgtaaccgta gaatttttct ttactttctt cccttttccc
      901 ggattctggc tcttaatctc gcctttttcg tattcattgg aagcttcttc tttatccgct
      961 tttttaatac cgagatcttt tttcttaagt tctgcctgtg cgtcttcaaa tgtcattccg
     1021 cgcacatccg gtactgttac catcttttct gtctttgaag aaagaccggc tcctttgaaa
     1081 aatcccgccg catttccgac aagaaacaga atcagcagcg cgatcacgac tcccgacacg
     1141 atcatcagta ttttcatgat ccgcttcgta tcgggattta catcattctt cttccgcctg
     1201 tctttttgac tgcgccttct gttatcataa tcattatcgt cgtcgtagtc ataatcgtcg
     1261 tcatcatcgt actcatcatc gtcatcgtac tcatcatcat aacgactgcc ctgaatccgt
     1321 tccagttctt ctgtcgacat aaccatcgtg cggtctgtct ccgctcctgt tcccgctccc
     1381 aaaacaacaa aatcgccgtc cggctctaca agcgatcttt tcagatccat aagaagagac
     1441 gcacagtccg gataacggcg tcccggattt ttctgtgtac acttcatgat aatgcactca
     1501 agactgtacg ggatattcgg aacttcttct gccggagacg taatctcttc ctgaagatgc
     1561 ttcaacgcga ccgacaccgt ggaatctccg tcgaacggta cgtgcccggt gatcatctcg
     1621 tacatcgtaa taccgatgga ataaagatcg ctcttctgat ctacgactcc gcccctcgcc
     1681 tgctccggag atgtgtaatg aaccgatccc ataacgcttg tgctgatcgt ctgtgtagac
     1741 gtggtcgcac gggcagtgcc gaaatccgtc acttttactt ttccgt
//
LOCUS       DBMDXF010000288         1776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13283_length_1776_cov_1.219701, whole genome shotgun sequence.
ACCESSION   DBMDXF010000288 DBMDXF010000000
VERSION     DBMDXF010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13283_length_1776_cov_1.219701"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="ACZPD4_08805"
     CDS             complement(<1..185)
                     /locus_tag="ACZPD4_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HIR1379190.1"
                     /translation="MEKTGKKISHVTGVLARSVRIIIENILREGGALQEIRMRIGQPL
                     TVMIDGEEQILPLKERA"
     gene            complement(314..1765)
                     /gene="miaB"
                     /locus_tag="ACZPD4_08810"
     CDS             complement(314..1765)
                     /gene="miaB"
                     /locus_tag="ACZPD4_08810"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1379191.1"
                     /translation="MENKTFITADAERNIPVTEPERQYYFIEKAKEKLTKLKEEEKRE
                     FTFCVTTFGCQMNARDSEKLVGILEQIGYVEEPDEEKADFVIYNTCTVRENANQRVYG
                     RLGQLGRIKKTNPHMMIALCGCMMQEPEVVEKLKKSYRFVDIIFGTHNIYKFAELIVS
                     RMNSGKMVIDIWKDTDKIVEDLPSERKYPFKSGVNIMFGCNNFCSYCIVPYVRGRERS
                     RDPKAIIREIERLAEDGVVEVMLLGQNVNSYGKTLEHPMTFAQLLREIEKIDKIERIR
                     FMTSHPKDLSDELIEVMAQSKKICRHLHLPVQSGSTRILEKMNRRYTKEHYLKLVEKL
                     RKAIPDISLTTDIIVGFPGETEEDFRETLDVVCKVQYDSAFTFIYSKRTGTPAAAMED
                     QISDDVVKDRFGRLLEEVQTISSKMCSRHEGTVQRVLVESCNEHDERLMTGRLSNNLL
                     VHFPGDESMIGTFADVSLDECKGFYYIGKIVGK"
BASE COUNT          471 a          440 c          252 g          613 t
ORIGIN      
        1 tgtgctcttt cttttaacgg aagtatctgc tcttcaccat cgatcataac agtaagcggc
       61 tgtccgatcc tcattcttat ttcctgaagt gctccgcctt cacgcaaaat attttcaata
      121 attatacgca ccgatctggc taaaactcct gtcacatgtg atattttctt ccctgttttt
      181 tccatcttgt ccacctcttt acacaatata tgtacatctc ccgaaaatat gaatcaaaaa
      241 agtttttaaa ataacaaaaa ggcaactatc cgatgtttct tacgaatagt cacctttgtt
      301 cttacgtctt tttttatttt cctacaattt ttcctatata ataaaagcct ttgcattcat
      361 caagactcac atccgcaaac gtaccgatca tagattcatc tccggggaaa tgtacaagca
      421 gattattgct cagacgacct gtcatcaacc tctcgtcatg ctcattgcaa gactctacaa
      481 gtactctttg aactgttcct tcatgccttg agcacatttt tgacgaaatc gtctgtactt
      541 cttcaagaag acgtccgaac cggtctttta caacatcatc cgatatctga tcttccatag
      601 cagccgcagg cgttcctgta cgcttagaat agataaatgt gaacgcacta tcatattgaa
      661 ctttacacac aacatccagc gtttcccgga aatcttcttc cgtctctccg ggaaaaccta
      721 caataatatc cgttgtcagt gaaatatccg gtattgcctt tctaagcttt tccacaagtt
      781 taagataatg ttcttttgta taacgtctgt tcattttttc taatatgcgc gtgcttcccg
      841 actgcacagg aagatgtaaa tgacgacaga ttttttttga ctgtgccatc acttcgatca
      901 gttcatccga aagatccttt ggatgggatg tcataaaacg gattcgttca attttatcaa
      961 tcttctcgat ttctcgaaga agctgtgcaa acgtcatcgg atgctccagt gttttcccat
     1021 aggaattgac attttgtcca agcagcatta cttctacaac accgtcctct gccaggcgct
     1081 cgatttccct tatgattgcc ttcggatccc tgcttctttc acgacctctt acataaggaa
     1141 cgatacaata actgcagaaa ttattacatc cgaacataat attaactcct gacttgaacg
     1201 gatacttcct ttcactcggc agatcttcta ctatcttgtc ggtatctttc catatatcga
     1261 tcaccatttt tcctgaattc atccgtgaga cgatcagctc agcgaattta tatatattat
     1321 gcgttccgaa aataatatct acaaaccgat aacttttttt cagtttttcc acaacctccg
     1381 gttcctgcat catacatccg cacagagcga tcatcatatg aggattcgtt tttttaatcc
     1441 tgcctaactg tcctaaacga ccgtacacac gttgatttgc attttcccga acagtacacg
     1501 tattataaat aacaaaatct gctttttcct catccggttc ttctacatat ccgatctgtt
     1561 ccaaaatacc aaccagcttt tccgaatctc tcgcattcat ctgacaaccg aatgttgtaa
     1621 cgcaaaatgt aaattctctt ttttcctctt ccttaagctt tgttaatttt tctttcgctt
     1681 tctctataaa ataatattgt ctttccggct ctgttaccgg aatatttctc tctgcatctg
     1741 ctgttataaa tgttttattt tccataactt cctctt
//
LOCUS       DBMDXF010000289         1774 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13302_length_1774_cov_1.903168, whole genome shotgun sequence.
ACCESSION   DBMDXF010000289 DBMDXF010000000
VERSION     DBMDXF010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13302_length_1774_cov_1.903168"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="ACZPD4_08815"
     CDS             complement(<1..1092)
                     /locus_tag="ACZPD4_08815"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIR1379192.1"
                     /translation="MNVLVINCGSSSLKFQLINAESEEVLAKGICERIGIDGRLTYQP
                     EGGEKEVSEKAMPTHTEAIQFVIEALTNPETGVVKSLDEIGAVGHRVVHGGEKFASSV
                     VITEEVKKAIEECNDLAPLHNPANLIGITACEKLMPNTPMTAVFDTAFHQTMPEKAYM
                     YGLPYNYYEDYKIRRYGFHGTSHSFVSKKAAEVMEKPYEELKTIVCHLGNGSSVTAVM
                     NGQSVDTSMGMTPLEGLVMGTRSGNIDPAIMEFIAEKEHLDIAGVMDVLNKKSGVFGI
                     SGELSSDFRDLTNAMNSGDKKAKIAMDVFSYNVAKYVGAYAAVMNGVDDIVFTAGIGE
                     NDDYVREQVCSYLGYLGVDFDKEANTGLRG"
     gene            complement(1167..1709)
                     /locus_tag="ACZPD4_08820"
                     /pseudo
     CDS             complement(1167..1709)
                     /locus_tag="ACZPD4_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
     gene            complement(1706..1774)
                     /locus_tag="ACZPD4_08825"
                     /pseudo
     CDS             complement(1706..1774)
                     /locus_tag="ACZPD4_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT          435 a          470 c          317 g          552 t
ORIGIN      
        1 tcctctcaaa cctgtatttg cctctttatc aaagtcaact ccgagatatc ccagataaga
       61 acacacctgt tctcttacat aatcgtcatt ctcaccgatt cccgcagtaa atacaatatc
      121 gtctacccca ttcattaccg ccgcatatgc tccgacatat ttggcaacat tataggaaaa
      181 tacgtccatt gcgatcttcg cttttttatc tcctgaattc atcgcatttg tcagatcacg
      241 gaaatcgctt gagagttctc ccgaaatacc gaacacacct gacttcttat taagaacgtc
      301 catcacgcct gcaatatcaa gatgttcttt ctctgcgata aattccatga tcgcaggatc
      361 gatatttccc gaacgtgttc ccattacaag accttccaac ggagtcattc ccatagaagt
      421 atctacagat tgtccgttca tgacagctgt cacacttgat ccgtttccga gatgacatac
      481 gatcgtcttc agttcttcat acggtttttc catcacttcc gccgctttct tagatacgaa
      541 actgtggctt gttccatgga agccgtaacg tctgatctta taatcctcat aataattgta
      601 tggaagtccg tacatatatg ctttttccgg cattgtctga tggaacgccg tatcgaatac
      661 agctgtcata ggtgtattcg gcataagttt ttcgcaggct gtaataccga tcaagtttgc
      721 cggattatga agcggcgcaa gatcattaca ttcctctatc gctttcttca cttcttctgt
      781 aatcacaact gaggatgcaa acttttctcc gccgtgtacg acacgatgtc ctacagctcc
      841 gatttcatcc aaactcttaa caacacccgt ttccggattt gtaagagctt caatcacaaa
      901 ctgaattgcc tctgtatggg tcggcatcgc cttttccgac acctcttttt ctccgccttc
      961 cggctgatac gtcagacgtc cgtcaattcc gatcctctca caaattcctt tcgccaaaac
     1021 ttcttctgat tccgcgttaa tgagctgaaa tttcaatgaa gaacttccac agttgattac
     1081 taatacgttc attacttctc ctccattttt gtttttataa actatataaa cagaaagttt
     1141 tactgaaact ctctatgtac tcgatcttac tctgcctgtg cctgcaccgc cgtaattgca
     1201 acaacaccca caatatcttc tgcgctgcat ccgcgtgaca gatcgttgac aggagctgcg
     1261 attccctgtg taagcggtcc gtatgcctct gcctttccaa gtctttgtac aagtttatat
     1321 ccgatattgc cggcatcaag atcagggaag atcaacacgt tcgctttacc tgccacttca
     1381 ctgcccggag ctttcgatgc tccgacttcc ggaacgatag cggcgtcaag ctgaagctct
     1441 ccgtctaatt ttaattccgg cgcaagttcg tgcgcgatct ttgttgcttc tacaacttta
     1501 tcgacatctg catgttttgc acttcctttt gtagaatgag aaagcatcgc tacaatcggt
     1561 tcttttcctg tcagcgtctc gaaagattct gcggaagaaa gtgcaatgtt tgccagtttt
     1621 tccggatcag ggttttgctc aagtccggca tctccgaaga tgaacgttcc gtccgctccc
     1681 atatcacagt caggaacaac cattaaaaag aggacacaag catttttccg tctgttttat
     1741 ccgggttttg gatatagtca agggctttgc tcaa
//
LOCUS       DBMDXF010000290         1757 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13442_length_1757_cov_2.094807, whole genome shotgun sequence.
ACCESSION   DBMDXF010000290 DBMDXF010000000
VERSION     DBMDXF010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13442_length_1757_cov_2.094807"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="ACZPD4_08830"
     CDS             <1..186
                     /locus_tag="ACZPD4_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1379193.1"
                     /translation="IAAVRVEYNNGLVIDGRLYTQGDGGLSIIDSVSKEIYGIATFEN
                     GEVKVTGSGFDGILKSK"
     gene            complement(264..>1757)
                     /locus_tag="ACZPD4_08835"
     CDS             complement(264..>1757)
                     /locus_tag="ACZPD4_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1379194.1"
                     /translation="DNTYQQEKMNNFISKLFDTSNLTDVQKEILMNLSLTSISGIRQR
                     LFKQFLDYNNSDINALIRQSWIIQNRPNGPASSQIHLHPVIKAAVINNTEPSLEKCSV
                     FINKIIEFLKAASADVIEDSMSNDLCDVLANAGLMFKYTSEHLGLMYDIALILWRSLM
                     YHESYSYLTKGLELLNKESSDDIELQLSYYETAGKVAVRLADYEKAITHYQSAITTIE
                     NSGQDFSERLATIYDKLGVVMRKASKYEQALDYFTKAQNIIDINHIKNPELTSDIYND
                     MGVIYINLDIFDKALANYQKAREIRESVENPDLEQIAYSYHNIGTVYQRQKKYADAIT
                     WHKKALEIRQEIYPDNEPIIAASLTMIGNDYTQAAKNDSSYHFNDAFEYFAKGLEIRK
                     LTLGETHPDTAWSYQSIGLWHFYQGEYEEAIENYLKCLSIRKTILQPSHAYTAEISYL
                     LGEAYLKINQIHSAKEHLLLAEKIQASLHKVKALEKTQHLLKECSLS"
BASE COUNT          476 a          305 c          388 g          588 t
ORIGIN      
        1 atcgcggcag tccgggtaga gtataataac gggctggtta tagacggaag attatatacg
       61 cagggagacg gcgggctttc gattatagac agcgtttcca aagaaatata cggaattgct
      121 acttttgaaa acggagaggt aaaagtgact ggaagtggat ttgacggtat tttaaaatcg
      181 aagtaaagga cgtataaaac gttgacaatt acatataaga agagcagata agagacaccg
      241 cagcttgcgg cgtctcttat cgtttatgaa agactgcatt ctttcaggag atgttgtgtt
      301 ttttcaagag cctttacttt atgaaggctt gcctgaattt tttctgcgag cagaaggtgt
      361 tcttttgcag agtggatttg gttgatcttt aaatatgctt ctcctaacaa gtatgagatt
      421 tcggcagtgt aggcgtgcga aggctgaagg atcgttttcc gaatgctgag acatttcaaa
      481 tagttttcaa tcgcttcttc gtattctcct tgataaaaat gccataatcc gatgctttgg
      541 taagaccatg ccgtgtcagg atgtgtttct cccaacgtga gctttcggat ttccaagcct
      601 tttgcgaaat attcaaaggc atcgttaaag tgataagaag aatcgttttt tgccgcctgt
      661 gtatagtcat tgccgatcat cgttaatgac gcggcaatga tcggttcgtt atcagggtat
      721 atttcctgac ggatctctaa cgctttttta tgccatgtga ttgcatctgc gtactttttc
      781 tgccgctgat atactgttcc gatattgtga taagaatagg cgatctgttc gagatcggga
      841 ttttcaaccg actcccggat ctcgcgggct ttttgataat tcgccaaagc tttatcgaag
      901 atatcaaggt taatatagat cacgcccata tcattataaa tgtcggatgt taattccgga
      961 ttttttatat ggttgatatc aataatattt tgagctttcg taaaataatc caaagcctgt
     1021 tcatattttg acgcttttcg cataactacc cctagtttgt catatatcgt ggcaagcctt
     1081 tcggaaaaat cttgtcctga attttcgatt gtcgtgattg ccgactgata gtgagtaatt
     1141 gctttttcgt aatcggcaag tctgacggca acttttcctg cggtttcgta gtaagacagt
     1201 tgtaattcga tatcgtcaga tgattctttg tttaatagtt ccaacccttt tgttaagtat
     1261 gaataagatt catgatacat aaggctgcgc cataagatta aggcgatgtc gtacattaag
     1321 ccgagatgtt cggaagtata tttgaacatg agacctgcgt ttgcaagaac gtcgcataaa
     1381 tcattcgaca tactgtcttc gatcacatct gcggaagccg ctttcagaaa ttcaattatt
     1441 ttgttgataa aaactgaaca tttttctaag gaaggttccg tattatttat tacagcagct
     1501 ttaatgaccg gatgcagatg aatctgtgat gaggccggac cgttgggtct gttttgtatg
     1561 atccagcttt gacggatcaa agcgttgata tctgaattat tataatctaa aaattgtttg
     1621 aataagcgct gtcttatgcc gcttatcgac gtaagagaga ggttcatcaa tatttctttt
     1681 tggacatcgg taagattgct tgtgtcaaaa agtttagata taaagttgtt cattttttcc
     1741 tgctgataag tattgtc
//
LOCUS       DBMDXF010000291         1750 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13514_length_1750_cov_1.346877, whole genome shotgun sequence.
ACCESSION   DBMDXF010000291 DBMDXF010000000
VERSION     DBMDXF010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13514_length_1750_cov_1.346877"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /gene="pckA"
                     /locus_tag="ACZPD4_08840"
     CDS             <1..1311
                     /gene="pckA"
                     /locus_tag="ACZPD4_08840"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR1379195.1"
                     /translation="KDIALKELSDKRLFVVDAFCGANKDTRMAIRFIVEVAWQAHFVK
                     NMFICPTEEELKEFKPDFVVYNASKAKVENYKELGLNSETAVMFNITSREQVILNTWY
                     GGEMKKGMFSMMNYYLPLKGIASMHCSANTDMNGENTAIFFGLSGTGKTTLSTDPKRL
                     LIGDDEHGWDDNGIFNFEGGCYAKVINLDKESEPDIYNAIKRNALLENVTLDENGKID
                     FDDKSVTENTRVSYPIDHIEKIVRPISAAPAAKNVIFLSADAFGVLPPVSILTPEQTE
                     YYFLSGFTAKLAGTERGITEPTPTFSACFGQAFLELHPTKYAEELVKKMKANNAKAYL
                     VNTGWNGTGKRISIKDTRGIIDAILNGDIANAPTKTIPYFNIEVPTELPGVDTNILDP
                     RDTYADASEWDEKAKDLAGRFIKNFAKYESNEAGKALVSAGPQL"
     gene            1568..>1750
                     /locus_tag="ACZPD4_08845"
     CDS             1568..>1750
                     /locus_tag="ACZPD4_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR1379196.1"
                     /translation="MIIKASATLRNDYSTISNLARATSEPIYITKNGEGDGVFMNIDA
                     FEKREQALKESGTQLLE"
BASE COUNT          594 a          296 c          389 g          471 t
ORIGIN      
        1 aaggatatcg ctctgaaaga gctgtccgac aaaagacttt tcgttgtaga tgcgttctgc
       61 ggtgctaata aagatacccg tatggcaatt cgttttatcg ttgaagttgc atggcaggca
      121 cattttgtta aaaacatgtt tatctgcccg acagaggaag aattaaagga attcaagccg
      181 gattttgttg tttacaatgc ttctaaagca aaagttgaaa actataaaga acttggcttg
      241 aattctgaga cagctgttat gtttaacatt acaagccgtg agcaggttat tctgaataca
      301 tggtacggcg gagagatgaa aaaaggtatg ttctctatga tgaactacta tctgccgctg
      361 aaaggaatcg cttctatgca ctgttccgca aacacagaca tgaacggaga gaatacagca
      421 attttcttcg gactttcagg aacaggtaag acaacgcttt ccacagatcc gaaacgtctt
      481 ttgatcggtg atgacgagca cggatgggat gataacggta tctttaactt tgaaggcgga
      541 tgttatgcaa aagtaatcaa ccttgataaa gagtcagagc cggatattta taatgcaatt
      601 aagagaaacg ctcttcttga gaacgtaact cttgatgaga acggaaaaat tgactttgat
      661 gataagagtg taacagagaa tactcgtgtg tcttatccga tcgatcatat tgaaaaaatc
      721 gtgcgtccga tatcggcagc tccggcagcg aaaaatgtaa ttttcctgtc agcagatgcg
      781 tttggagtgc ttccgccggt atcgattctt acaccggagc agacagaata ttatttcttg
      841 tcaggattta cagcaaaact tgcaggtacg gagcgtggta tcacagagcc gacaccgaca
      901 ttctcagcat gttttggaca ggcatttctt gagcttcatc cgacaaaata tgctgaagag
      961 ctcgttaaga aaatgaaagc taacaacgcg aaagcatatc ttgtaaatac aggatggaac
     1021 ggaacaggaa aacgtatctc tattaaagat actcgtggta tcatcgatgc aattttgaac
     1081 ggagatatcg caaatgcgcc gacaaaaact attccgtact tcaacattga agttccgaca
     1141 gagcttccgg gagttgacac aaacattctt gatcctcgtg acacatatgc agacgcttcc
     1201 gaatgggatg aaaaggcaaa agatcttgca ggaagattta ttaaaaactt tgcaaaatat
     1261 gagagcaacg aggcaggaaa agctcttgtt tcagctggtc cgcagttata agaacagttt
     1321 tttcgttaat caaagaaaat catattcagc agagaatatg atgattgtcg atataagaaa
     1381 tgtgaaaaag aaatcgctga agttttatac ttcagcgatt tctttgtata ggagatgaga
     1441 caaagtctgg attttccaaa gacttcttct ttcataaatt ggatttactt gataatatat
     1501 aaaaaagaag atataattat atcaataaaa tgatataaaa ataaagaaac atgaaagggt
     1561 gaagaaaatg attattaaag cttcggcaac attacgaaac gattattcta cgatttccaa
     1621 tcttgcaagg gcaacgagtg aaccgattta tataacaaaa aatggcgagg gtgacggagt
     1681 atttatgaat attgatgcct ttgagaaaag ggaacaggca ttgaaggaat cggggacgca
     1741 gctgctggaa
//
LOCUS       DBMDXF010000292         1717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13854_length_1717_cov_1.584777, whole genome shotgun sequence.
ACCESSION   DBMDXF010000292 DBMDXF010000000
VERSION     DBMDXF010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13854_length_1717_cov_1.584777"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="ACZPD4_08850"
     CDS             complement(<1..347)
                     /locus_tag="ACZPD4_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1379197.1"
                     /translation="MSGTQTSENVLEMRGITKVYPNGIIANKDVNFSVRRGEIHALSG
                     ENGAGKTTLMKMLFGLEKIDGGEIYIKGKKIENISSPMDAIKNGVGMVHQHFMLVDDL
                     TVAENMVMGMEPR"
     gene            complement(424..>905)
                     /locus_tag="ACZPD4_08855"
     CDS             complement(424..>905)
                     /locus_tag="ACZPD4_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1379198.1"
                     /translation="KDTVKAKELALQIYSQGADFLYTPAGQTIMSIIEAAKEKNGYVI
                     GCDNDQYTLIKETDPEGAALTITSVMKPVGDTIFDAVKAYADGELQFGTGTNLGVKEG
                     GSVLAYHENYMNTVPQEIRDQVEQIKQDIIDGKIEIWQASEKTPEEVTAYRNGFLPG"
     assembly_gap    906..915
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<916..1603)
                     /locus_tag="ACZPD4_08860"
     CDS             complement(<916..1603)
                     /locus_tag="ACZPD4_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1379199.1"
                     /translation="MMMKKMLAMILTAALILGLTACGAGESPSQSSSITGGSSTTTSA
                     SSKGTDYSKLKICVLIHGLLGDNGYFDSAAQGAKRLEEELGCKVQVVEMGTDSSVYEA
                     NLADASESGYDLIIMGGTRYYEMIYNTALQYPDQKYIMYDNIGNFTDYENSNLYAYGF
                     AAEQGSFLAGALAALCTKDTADFENATEDALIGVLCSADEPMLNNFVCGYLQGAAYVD
                     PEIKVLSAYIG"
BASE COUNT          411 a          455 c          346 g          495 t
ORIGIN      
        1 ttgcggggct ccatccccat gaccatgttt tcggccaccg tcagatcatc cactaacata
       61 aaatgctgat gaaccatgcc cactccattt ttgatcgcat ccatgggtga agaaatattt
      121 tctatctttt ttcccttgat gtagatttcc ccgccatcga tcttttctaa gccgaacagc
      181 attttcatca gggtagtctt ccccgctccg ttttcgcccg agagggcatg aatctctcct
      241 cttcggaccg aaaaattcac atctttattg gcgatgattc cgtttggata aaccttggtg
      301 attccccgca tctccaagac attttctgat gtttgcgtcc cagacatctg cccctccttc
      361 tcctgtaaat caaaggataa gaaagcggcg gccgccagac cgccgcctct aaaaagtcag
      421 ttgttaaccc ggcaaaaagc cgttgcggta tgcagtcact tcttcgggag tcttttccga
      481 ggcttgccat atctcgattt ttccatcgat aatatcctgc ttgatctgtt ccacttggtc
      541 gcggatctcc tgcggaacag tattcatata attttcatga taggctaaaa cagaccctcc
      601 ctctttgacg cccaaattcg tccctgtgcc aaattgcagc tctccatctg catatgcctt
      661 gacagcatca aagatggtat cccccacggg tttcatcaca gaagtgatgg tgagcgccgc
      721 cccctcggga tccgtctcct tgattaacgt gtactgatcg ttgtcgcagc cgatcacata
      781 tccattcttt tctttcgcag cctcaataat ggacataatg gtttgtcccg ccggcgtata
      841 gaggaaatcc gccccttgag aatatatctg aagtgctaac tctttggcct ttaccgtgtc
      901 tttatnnnnn nnnnnaaccg atgtaggcag acagcacctt gatctcgggg tcaacataag
      961 ccgccccctg cagatagccg catacaaagt tgttcaacat cggctcatca gcagagcaca
     1021 acacgccgat taaggcatcc tctgttgcgt tttcaaaatc agcagtgtct tttgtgcaaa
     1081 gcgctgcaag cgcacccgcc aaaaatgacc cctgctctgc cgcaaatcca taagcgtata
     1141 gattgctgtt ttcatagtcg gtaaagttcc ctatattgtc atacataata tatttttgat
     1201 cagggtactg aagcgcagta ttataaatca tctcataata gcgggtcccg cccataatga
     1261 tcagatcata tccagactca gaggcatccg ccaaatttgc ctcgtaaaca gaagagtccg
     1321 tgcccatttc cacaacctgg actttgcacc ccagttcttc ctccagacgt tttgcccctt
     1381 gggctgcgga atcgaaatat ccattgtcgc ccaagagacc atggatcaga acacagattt
     1441 tcagtttaga ataatcggta cctttactgg aagctgaagt ggtggtggat gagcccccgg
     1501 taatgctcga cgactgggag ggactttccc ccgcgccgca tgctgtgagc cccaggatga
     1561 gagctgctgt caagatcatt gcgagcattt ttttcatcat catttcctcc ttgtgtgtag
     1621 aaaaatcgat aaattgtttt atcattcttg ataaaacgat taatctattt aggagtgtat
     1681 cattgccact gtgaatttgc aatgtttttt ctttttt
//
LOCUS       DBMDXF010000293         1714 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13885_length_1714_cov_1.569126, whole genome shotgun sequence.
ACCESSION   DBMDXF010000293 DBMDXF010000000
VERSION     DBMDXF010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_1714_cov_1.569126"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            41..364
                     /locus_tag="ACZPD4_08865"
     CDS             41..364
                     /locus_tag="ACZPD4_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379200.1"
                     /translation="MKKFMKEITLFIIQLLIFYLFPLFAKQIDAIGMVLFLITATFVL
                     SALMGIISTNKIKYFYPLITAILFIPSVFIYYNESALIHSVWYFVVSVVGTAIGTVIT
                     KLFAK"
     gene            complement(476..703)
                     /locus_tag="ACZPD4_08870"
     CDS             complement(476..703)
                     /locus_tag="ACZPD4_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HIR1379201.1"
                     /translation="MTGVVIENKNQIKIEATEKDFVDSHGKYFVIRFEKGKENRNDDF
                     LVGDQVIVKYDGIIYESNPAVIDAKSVKRKE"
     gene            complement(946..>1714)
                     /locus_tag="ACZPD4_08875"
     CDS             complement(946..>1714)
                     /locus_tag="ACZPD4_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR1379202.1"
                     /translation="SEDESVKGIWCVPKYSNPQGITYSDDTVYRFANLKPAAKDFRIF
                     WDNAYCVHHLYEDKQDYLLEILMECKKAGNPDMVYKFSSTSKISFPGSGIAALAASEA
                     NLKDVRNMMKVQTIGHDKVNQLRHVRFFKNIHGIVEHMKKHADILRPKFEAVLDVLER
                     ELGGLEIGSWIAPHGGYFISFDAMEGCAKAIVAKAKEAGLVMTGAGATFPYGKDPHDS
                     NIRIAPSYPTPEELSVAAEIFVLSVKLVSIDKLLGNL"
BASE COUNT          510 a          388 c          252 g          564 t
ORIGIN      
        1 cctactctca aattttgtcg tagtagaaag gagctgtcat atgaaaaaat ttatgaaaga
       61 aataacatta tttataattc aattattgat tttttatctg tttcctttat ttgcaaaaca
      121 gattgacgca ataggcatgg ttctcttcct tatcactgcg acatttgtat tatctgctct
      181 aatgggaatc atctcaacta acaagatcaa atatttctat ccgcttataa cagcaatact
      241 ttttattcct tccgtgttca tttattataa cgaaagcgct cttattcatt ctgtctggta
      301 ttttgtcgtt tccgttgtcg gaacagcgat cggaacggtc ataaccaaat tatttgcaaa
      361 ataatcatga aaccacagca tgatagatgc aaatagggag acacattaat aaaaaatatc
      421 tgcttctatt cacctgaata agctatgaaa aaaggatata ttctttttcc atagcttatt
      481 cttttcgttt aacggacttt gcatctatca ctgccggatt tgactcatat attataccgt
      541 catatttaac aataacttga tcccccacaa gaaagtcatc gtttctgttt tccttgcctt
      601 tttcaaacct tatcacaaaa tatttcccgt gagaatccac aaaatctttt tctgtagctt
      661 ctattttaat ctgattctta ttttcgatta ctacacctgt catcgaatgc ctgttaaaac
      721 atacattcca caataaaata ctcaaaaaag caaaaatcac aactgccaaa aatattttta
      781 ttcttttaaa agaatgactg cctgtcatgc gatacacctt cctctctttt cgcaaataac
      841 aaaaatggga gacgcacttt tttcatattc aactgtctgc tcaacagaaa aatatctttt
      901 ttgtgcaatc tcccatttta tttactgtat ttaaaatcgt ctgttttata aatttccgag
      961 caatttatct atacttacaa gtttcacact cagtacaaaa atttccgccg caacggacaa
     1021 ttcttccgga gtcggataag acggcgcaat acggatatta ctgtcatgag gatcttttcc
     1081 atacgggaat gtcgcccctg ctccggtcat tacaagacct gcctctttcg cctttgcaac
     1141 gatcgccttt gcgcagcctt ccattgcatc aaaagagata aagtatccgc cgtgaggcgc
     1201 gatccatgat ccgatttcca gtcctcccaa ttctctttcc agaacgtcca gaaccgcctc
     1261 aaacttcggc cggagaatat cggcatgttt cttcatatgc tctacgattc cgtgaatatt
     1321 tttgaaaaat ctcacatgac gcaactgatt tactttatca tgtccgatcg tctgaacttt
     1381 catcatatta cgaacgtctt ttaagtttgc ttccgacgcc gccaaagccg cgatgcctga
     1441 tcccgggaag ctgatcttgg aagttgaaga gaacttataa accatatcag gatttcccgc
     1501 ttttttacat tccatcaaaa tctcaagaag atagtcctgc ttatcttcat acagatgatg
     1561 tacgcaatat gcattatccc aaaaaatacg gaaatctttt gcagccggtt tcagattagc
     1621 aaaacgatat accgtatcat cagaatatgt gattccctgc ggattagaat acttcggcac
     1681 acaccagatt cctttgaccg actcatcttc actg
//
LOCUS       DBMDXF010000294         1711 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13911_length_1711_cov_1.675155, whole genome shotgun sequence.
ACCESSION   DBMDXF010000294 DBMDXF010000000
VERSION     DBMDXF010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13911_length_1711_cov_1.675155"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(125..>1711)
                     /gene="thrS"
                     /locus_tag="ACZPD4_08880"
     CDS             complement(125..>1711)
                     /gene="thrS"
                     /locus_tag="ACZPD4_08880"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1379203.1"
                     /translation="KAKEAEALYKEYQKASASLSAAQKTQREEAIAYFKEKNEPYKVE
                     LIEDLPEGETISFYSQGEFTDLCAGPHLMTTKPVKAFKLTSLAGAYWRGDEHNKMLQR
                     IYGTAFSKKAELEEYLTMIEEAKKRDHRKLGKELGLFMMREEGPGFPFFLPNGMVLKN
                     TLLDYWREIHRRAGYVEINTPIMLSRHLWETSGHWDHYKENMYTTVVDEEDFAIKPMN
                     CPGGILVYESEPRSYRDLPIRMGELGLVHRHEKSGQLHGLMRVRCFTQDDAHIFMTPE
                     QIKDEIKGVVKLIDEVYSLFGFKYHVELSTRPDDSMGSDEDWEMATDALRSALDDIGL
                     PYIVNEGDGAFYGPKIDFHLEDSIGRTWQCGTIQLDFQLPLRFELEYTGADGEKHRPI
                     MIHRVVFGSIERFIGILIEHFAGAFPTWLAPVQVKVLPISDKYLDYAGKVLEDLKEAG
                     IRAEIDSRAEKIGYKIREAQMKKIPYMLVVGAKEEEEGKVSVRSRFAGDEGQCGLEEF
                     IEQIKEEVAAKKLREKVQDK"
BASE COUNT          432 a          438 c          295 g          546 t
ORIGIN      
        1 cttacaaaaa agaagaacct taaaacagat gttttttctt ttacattttg atcccaatac
       61 aaaaaagagt tcagcagtat gatagtatca tctgttctga actcttctta atttatttta
      121 tgatttattt atcctgcact ttctctctga gtttctttgc tgcaacttct tctttaatct
      181 gttctataaa ctcttccaga ccacactgac cttcatctcc ggcaaatctg cttcttacag
      241 atactttgcc ttcctcttct tccttcgctc ctacaacgag catatacgga atctttttca
      301 tctgtgcctc gcggatctta tatccgatct tctccgcacg actgtcaatt tctgcgcgaa
      361 tacccgcctc cttcagatct tcaagcactt tacctgcata atcaagatat ttgtcagaga
      421 tcggaagaac tttcacctgt accggagcaa gccatgtcgg gaatgcgccc gcaaaatgct
      481 cgatcagaat tccgatgaaa cgttcgatag aaccgaatac aacgcggtgg atcataatcg
      541 gtctgtgctt ctctccgtcc gctcctgtat actcaagttc aaatcgaagc ggaagctgga
      601 agtcaagctg gatcgtaccg cactgccacg ttcttccgat agaatcttcc agatggaagt
      661 cgatcttcgg accgtaaaaa gctccgtccc cttcatttac aatatacgga agtccgatat
      721 catcaagcgc gcttctaaga gcatctgtcg ccatttccca atcttcatca cttcccatac
      781 tgtcgtccgg ccgtgtagaa agctcgacat gatacttaaa tccaaacaaa ctatacactt
      841 cgtcgatcag ttttacaacg cctttgatct catctttaat ctgttccggc gtcatgaaaa
      901 tatgcgcatc atcttgtgta aaacagcgaa ctctcattaa tccgtggagc tgacctgatt
      961 tttcatgacg gtgcacaagt cccaactctc ccatacggat cggaagatca cggtaagaac
     1021 gcggctctga ctcatacaca agaattccgc ccggacagtt catcggttta attgcgaaat
     1081 cttcctcatc aactactgtt gtatacatat tttctttata atgatcccag tgacctgatg
     1141 tttcccataa atggcgactt aacataatcg gcgtattaat ttctacatat cccgctctgc
     1201 ggtgaatctc tctccagtaa tcaagcagtg tgtttttcag aaccataccg ttcggaagga
     1261 aaaacggaaa tcccggtcct tcttcgcgca tcataaacag accgagttct tttccaagtt
     1321 ttctgtgatc acgctttttc gcttcctcaa tcatcgtcag atattcttca agctctgcct
     1381 ttttggaaaa agcagttccg taaattctct gaagcatttt attatgttcg tcgcctctcc
     1441 aataagcgcc tgcaagactt gttaatttaa atgctttcac cggttttgtt gtcataagat
     1501 gcggacctgc gcacagatcg gtaaattctc cctgtgaata gaaactgatc gtttcgcctt
     1561 ccggaagatc ttcgatcaat tctactttat aaggctcatt tttttcttta aaatacgcaa
     1621 tcgcttcttc tctttgcgtt ttctgtgcgg ccgacaggga agccgaggct ttctggtatt
     1681 ctttataaag tgcttccgct tcttttgctt t
//
LOCUS       DBMDXF010000295         1711 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13913_length_1711_cov_1.381988, whole genome shotgun sequence.
ACCESSION   DBMDXF010000295 DBMDXF010000000
VERSION     DBMDXF010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13913_length_1711_cov_1.381988"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1711)
                     /locus_tag="ACZPD4_08885"
     CDS             complement(<1..>1711)
                     /locus_tag="ACZPD4_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HIR1379204.1"
                     /translation="EGTILVHYTSTSDQAIQSLFSVSNAKAGHENRHFHVYIRPEGVL
                     GCEIRNESAMNYGFKAANAVKADYKGKPAENIIALQADKEKGTYQLFANGEKVLTVDA
                     AALGGYRFISEITGLDTVSLGATKRGGINKYTFGGNIHKIEVYETPLTDEELIEETKK
                     TAYPELQQIFHKNDGTGANYYRIPALLTLKSGTVISAADARFGGTHDSPNNIDIAVAR
                     SEDGGKNWSKPELPFHYEDYEDNTLEIPVGTQTRVNQSASFIDPVLLQDEETERVFLI
                     SDAMAAGYGSPQAVTGSGYKEIQGKKYLKLQKAGETDYKYTVRENGVIYNDTTNQPTE
                     YSLNSNFEILKDNVLQTVKQKSSRFDPTNGSGQLVTDETDKDVPMNIMYADAVFKALP
                     TTWLYMKYSDDDGKTWSDPILLNGMVKAEDSRVLVTGPGRGMQIKNGEYKGRLIVPVY
                     DTAQSGIIYSDDHGATWNYAKGPSTKKAAMSESQIVEMPDGTLRVYARSTGSKIAEAV
                     SLDGGKTWTEAAYVPGMTQPGWGSQLSVIRYGGLIEGKPALILSTPAGVGSYRRDGRV
                     KIGL"
BASE COUNT          388 a          457 c          288 g          578 t
ORIGIN      
        1 tcagcccgat cttcactctt ccatctcttc gataactgcc cacacctgca ggtgtagata
       61 aaatcaacgc cggttttcct tctatcagac cgccatagcg gatcacggat aactgcgatc
      121 cccatcccgg ctgggtcatt cccggtacat acgccgcttc tgtccatgtt tttcctccgt
      181 ccaggctcac tgcttccgct atcttagaac ctgttgatct cgcataaaca cgaagcgttc
      241 catccggcat ttctacgatc tgtgattctg acatcgccgc tttcttcgta gacggtcctt
      301 ttgcataatt ccatgttgct ccatgatcgt cgctgtaaat aattcccgac tgtgccgtat
      361 cataaaccgg tacgatcaac cgccccttat attctccgtt tttaatctgc atgcctcttc
      421 ccggacctgt gacaagcact cttgaatctt ctgctttgac cattccgttt aaaagtatcg
      481 gatcagacca tgtttttccg tcatcatcac tgtacttcat atacagccat gtcgtaggca
      541 atgctttgaa tactgcatct gcatacataa tgttcatcgg aacgtctttg tctgtctcat
      601 ctgttacaag ctgtcccgaa ccgttcgtag gatcgaatct tgacgatttt tgtttgactg
      661 tctgcaatac attatccttc aaaatttcaa aattactgtt cagagaatac tctgtcggct
      721 gattcgttgt atcattatag ataacaccat tctccctcac tgtatattta taatctgttt
      781 ctcctgcctt ttgcagtttc aagtattttt tcccttgaat ttctttatat ccgcttcctg
      841 taactgcctg cggagaacca tatccagccg ccatagcgtc cgatatcaaa aatacacgtt
      901 ctgtctcttc gtcctgcaaa agaaccggat cgataaatga tgcactttga tttactctcg
      961 tctgcgttcc taccggaatt tctaatgtat tatcttcata atcctcatag tggaatggaa
     1021 gctccggctt gctccagttc tttcctccat cttcgcttct tgccactgca atatcaatat
     1081 tattcggtga atcgtgcgta ccgccaaacc ttgcgtctgc cgcgctgatc actgtaccgc
     1141 ttttcagtgt caaaagcgcc ggaatccggt aatagttcgc gcctgttccg tcatttttat
     1201 ggaaaatctg ctgtaattca ggatatgcgg tcttcttcgt ttcctcgatc agttcttcat
     1261 ctgttaaagg agtctcatac acttcgatct tatggatatt tcctccgaaa gtatatttat
     1321 taattcctcc cctttttgtt gcccccagag aaacagtatc taatcccgta atttcagaaa
     1381 taaagcggta tcctcccaat gcagcggcgt ctaccgtcag cactttctct ccgtttgcaa
     1441 acaactgata cgttcctttt tctttatctg cctgaagcgc aattatattt tctgcaggtt
     1501 ttcctttata atctgccttc actgcatttg ccgctttaaa tccataattc atcgcgctct
     1561 catttctgat ttcgcagcca agtactcctt caggtctgat ataaacatga aaatgtctgt
     1621 tttcatgtcc tgcttttgca ttggaaacac taaacaaaga ctgaattgcc tgatcgcttg
     1681 tcgatgtata gtgtactaaa atcgtacctt c
//
LOCUS       DBMDXF010000296         1707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_13949_length_1707_cov_1.889788, whole genome shotgun sequence.
ACCESSION   DBMDXF010000296 DBMDXF010000000
VERSION     DBMDXF010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13949_length_1707_cov_1.889788"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="ACZPD4_08890"
     CDS             complement(<1..493)
                     /locus_tag="ACZPD4_08890"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HIR1379205.1"
                     /translation="MSEKNKRIAVCTRKTKETDITLSVNLDGQGKNKIDTGIAFFDHM
                     LDGFARHGLFDLCIRVHGDLQVDDHHTIEDTGIVLGTAIKEAAADKKGIRRYGSCILP
                     MDEVLVLCAIDLSGRAYYSSDAEFSCEKIGDMSSEMVREFFYAVAVNSGMNLHFQVLT
                     NGNS"
     gene            complement(498..>1707)
                     /gene="hisD"
                     /locus_tag="ACZPD4_08895"
     CDS             complement(498..>1707)
                     /gene="hisD"
                     /locus_tag="ACZPD4_08895"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1379206.1"
                     /translation="VKAILDNVKKRGDEALFEYTEKFDGFCLDQQSVLVKEEEIEEAY
                     TQVDRGLIEIIRKALKNIETYHEKQRQYSWFDSKPDGTILGQKVTALDRVGVYVPGGK
                     AAYPSSVLMNIVPAKTAGVREIIMVTPPGKDGKVTPTTLVAAKEAGADKIYKVGGAQA
                     IAALAYGTESISKVDKIVGPGNIYVALAKKAVYGHVSIDSIAGPSEILVLADETANPR
                     YVAADLLSQAEHDEMASAILVTVSMELAEKVSEQTELFISQLSRGEIIRKSLDNYGHI
                     LVADTMDEAIDAANEIASEHLEIMTASPFEVMTKIRNAGAIFIGENSSEPLGDYFAGP
                     NHVLPTNGTAKFFSPLSVDDFIKKSSIISYSREALEKIHSDIERFAEAEHLTAHANSI
                     KVRFESERGE"
BASE COUNT          438 a          423 c          308 g          538 t
ORIGIN      
        1 ggctgtttcc gtttgtcaga acctgaaaat gaaggttcat tccgctgttt acagcaactg
       61 cataaaaaaa ttctcttacc atctcagaag acatatctcc gatcttttcg caggaaaact
      121 ctgcgtctga agaataataa gcccgtccgg acaggtctat agcacagagc accaacactt
      181 catccatggg aagaatacag cttccgtacc gccgtattcc ttttttgtct gcagctgcct
      241 ctttaatggc tgttcccaga acaatccctg tatcttctat ggtatgatga tcgtctacct
      301 gcagatcccc gtgaacacgg atacacagat caaaaagtcc atgtctggca aatccgtcaa
      361 gcatgtgatc aaaaaaagca atccccgtat ctattttatt tttcccctga ccgtctaaat
      421 taacagacaa ggtgatatct gtctcttttg tttttcgcgt gcaaaccgct atacgtttgt
      481 ttttttcact cataatatta ctctcctcgt tctgattcaa aacgaacctt tatcgaattt
      541 gcatgagctg tcaaatgttc agcttcggca aaccgttcga tatccgaatg aattttttca
      601 agtgcttccc ttgagtagga aataatactt gattttttaa taaaatcatc tacagaaaga
      661 ggagagaaaa attttgcagt tccgtttgtc ggaagaacat gattcggtcc cgcaaaataa
      721 tcgccgagcg gttcgcttga attttccccg ataaatatag cgcctgcatt tcggatcttt
      781 gtcatcactt caaatgggga tgcagtcatg atttcaaggt gctctgatgc aatttcattt
      841 gcagcgtcaa tcgcctcatc cattgtatcc gccacgagaa tatgtccgta attatcgaga
      901 gattttctga taatctctcc ccgtgataat tgactgataa aaagttctgt ctgctcggaa
      961 actttctctg ccaattccat acttactgtc actaaaatcg cgcttgccat ttcatcatgt
     1021 tccgcctgag acaaaagatc tgcggcaaca taacgcgggt tcgccgtttc atctgcaagc
     1081 acgagtattt cactcggacc tgcgatagag tcaatactaa catgaccata taccgccttt
     1141 tttgccagtg cgacataaat attccccggg ccgacgatct tatctacttt cgatatgctt
     1201 tccgttccgt aagcaagagc ggcaatcgcc tgcgcgcctc cgactttata gattttatcc
     1261 gctccggctt cttttgccgc aacaagtgtg gtgggagtta ctttcccgtc ttttccgggc
     1321 ggagtaacca ttatgatttc tcttactccc gctgtttttg ccggaacgat attcataaga
     1381 accgaagaag ggtatgcagc tttccctccc ggcacatata cgcctacccg atcgagagcc
     1441 gtcactttct gaccaaggat cgttccgtca ggtttactgt caaaccaact gtactgtctc
     1501 tgtttttcat gatacgtctc aatatttttc aaagcctttc tgattatctc aatcaatcct
     1561 ctgtcgactt gagtataagc ttcttcaatt tcttcttctt ttacaagaac agactgctga
     1621 tcaagacaga agccgtcgaa tttttctgta tattcaaaaa gggcttcatc tcctcttttt
     1681 ttcacattat ctaagattgc ttttacc
//
LOCUS       DBMDXF010000297         1693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_14104_length_1693_cov_1.067211, whole genome shotgun sequence.
ACCESSION   DBMDXF010000297 DBMDXF010000000
VERSION     DBMDXF010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14104_length_1693_cov_1.067211"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="ACZPD4_08900"
     CDS             <1..262
                     /locus_tag="ACZPD4_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1379207.1"
                     /translation="EGKLVGMKMPVRYVVEMFMDRIAACKVYKGAAYKDSDPLIYYNS
                     GKAGDMMHKDTRKLLEKLLHMLAEQGEETVFCYVRREILKKK"
     gene            complement(308..787)
                     /locus_tag="ACZPD4_08905"
     CDS             complement(308..787)
                     /locus_tag="ACZPD4_08905"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIR1379208.1"
                     /translation="MNKITSFTIDHIRLIPGIYVSRKDSVNGHIITTFDIRMTSPNDE
                     PVMNTAEIHTIEHLGATFLRNHEVYKDQVIYFGPMGCRTGFYLLLADDLTSSEILPLM
                     KEMFSFIASFKGEVPGASAKDCGNYLDMNLPMANFLARKFLEEVLTDIREEQLNYPV"
     gene            1043..1657
                     /locus_tag="ACZPD4_08910"
     CDS             1043..1657
                     /locus_tag="ACZPD4_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1379209.1"
                     /translation="MLLYIVRHGETDWNKAGKVQGRTDIPLNERGRYLAEATAEGMKD
                     VRIDFCYTSPLIRAKETAQIILGEREIPLVEEKRIEEICFGKCEGMKFRGEITDPGSE
                     AFQRFFTDTENYVPSEGAESISELYERTGCFLSEIADREDLKDSHILISTHGAAMTAL
                     LNRIKGNMQIKDFWKYKVPKNCAVSVVEIKGKDIIIKEEGLIYY"
BASE COUNT          574 a          245 c          422 g          452 t
ORIGIN      
        1 agaagggaaa ctggtaggaa tgaagatgcc ggtcagatac gtcgtagaga tgtttatgga
       61 tcggatcgcg gcatgtaaag tatataaagg agcagcatac aaagacagcg atccactgat
      121 atattataac agtggaaaag cgggagatat gatgcataaa gatacgagga aacttttgga
      181 aaaacttttg catatgcttg cggaacaagg ggaagagaca gtgttttgct atgttcgccg
      241 ggaaatattg aaaaagaaat gaaagatata aaaataagcc gttaaaaaac ggcttattta
      301 atatttatca gaccggataa ttcagttgtt cttcccgaat atccgtcaat acttcttcaa
      361 gaaattttcg ggcaaggaag tttgccatcg gaagattcat atcaagataa tttccgcagt
      421 ctttggcaga tgctccaggg acttctccct taaaagacgc gataaaagaa aacatttctt
      481 tcataagagg taagatctca gaagaagtca gatcatctgc aagaagaaga tagaaaccgg
      541 ttctgcatcc cataggacca aagtagatca cctgatcttt atatacttca tggttgcgga
      601 gaaatgtggc gcccagatgt tcgattgtgt ggatttcggc agtattcatg acaggttcat
      661 cattcgggct tgtcattcgg atgtcaaatg tcgtgatgat atgaccgttg acagagtctt
      721 ttcttgatac atagattccg ggaatgaggc ggatgtgatc gatcgtaaaa cttgtgattt
      781 tattcatatt gatgttcctt tcttatgttt gttgagccat acgccctgat tttttaatct
      841 tttatgtaag tgttatgatg caacatcgtt attatagaca tgaccgtgaa aaaagacaag
      901 agagaaagtt gataaaagaa ggcggaaaag ggtaagggat cggaaaaaag atctattgat
      961 catagtggga aaatgtgaaa aataataaaa aatctgcaat atacaaaaag cggataagaa
     1021 aaaagagaag gagagaaaag aaatgctgct ttatattgtt cgtcatgggg aaacagattg
     1081 gaataaggcg ggaaaagttc agggacgtac ggatattccg ttgaatgaaa ggggcaggta
     1141 tcttgcggaa gcgactgcag aagggatgaa ggacgtccgc atcgattttt gttatacaag
     1201 ccctttgatc cgggcgaaag agacggcgca gatcatactt ggggaaagag aaattcctct
     1261 ggttgaagaa aaacgcattg aggagatttg tttcggaaag tgtgaaggaa tgaagttccg
     1321 tggggaaatc acagatccgg ggagcgaagc gtttcagcgc ttttttacag atacggaaaa
     1381 ctatgttcct tctgaaggag cagagtcgat cagtgaatta tatgaaagaa caggctgctt
     1441 tttgtcggaa attgcagatc gggaggattt gaaagacagt catattttga tttcaaccca
     1501 tggagcggcc atgacagctt tgttgaaccg gataaaagga aatatgcaga taaaagattt
     1561 ttggaaatat aaagtgccga aaaactgtgc tgtttccgta gtggaaataa aaggaaagga
     1621 tatcataatt aaagaggaag gtctcatata ctattaaagt ttacaaataa agtatgcttt
     1681 tgaaggagtt ttt
//
LOCUS       DBMDXF010000298         1656 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_14521_length_1656_cov_2.098392, whole genome shotgun sequence.
ACCESSION   DBMDXF010000298 DBMDXF010000000
VERSION     DBMDXF010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14521_length_1656_cov_2.098392"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            38..877
                     /locus_tag="ACZPD4_08915"
     CDS             38..877
                     /locus_tag="ACZPD4_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1379210.1"
                     /translation="MYENLLNPNEFSSISGVSKQTLLFYEKKNVFKPIFKNEKGFRFY
                     SLEQLDIIVTIQALQTIGLSLKEIQNYIENRNADLIYDLYSNRMIPLKQTVNKYNRTI
                     EMMHVKLDLINKAKSVLLDTIYLEYRPAIQIVKSKEIPADASDTLQYKILGEHMAYRK
                     EKDYSLGHAVAGIVDMKTALNPNINKTQYKYYYTILPSDHDEKDYDIIESGTYIVMYH
                     KGPYNQTYQAYPFMIDYAGKNHFEIESMIYDESLIDETTESDPANYITQISIKIKEVK
                     GSD"
     gene            complement(869..1507)
                     /locus_tag="ACZPD4_08920"
     CDS             complement(869..1507)
                     /locus_tag="ACZPD4_08920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1379211.1"
                     /translation="MKKKYVLFDFDGVIADTEESNSHYLGLALKEFGVELTEKDKQRL
                     IGTHDQELLIEFLSRAPRKVTVEQLTRRRKELGNTYENGNIAPIPGIVPLIQGLRQSG
                     VKTALVSSTATHLIIMGLNRMQMTDLFDVIVCGDMCAERKPDPECYLKAMGLLGAVPQ
                     ECLVFEDSSVGIHAAKQAGIEVAAFTGSGNGQDVSEADYVVSSYEESKRVLI"
BASE COUNT          539 a          374 c          275 g          468 t
ORIGIN      
        1 ataaaatttc cattttacgc aggaaaggat tgctattatg tacgaaaatc tattaaatcc
       61 aaatgaattt tccagtattt caggagtttc caagcaaact ttactttttt acgaaaagaa
      121 aaatgtattt aaaccgatat ttaaaaatga aaaaggattc cgattttaca gtttagaaca
      181 acttgatatt atagtaacta tacaggcctt acagacaatc ggcttgtctc tcaaagaaat
      241 tcagaattat atagaaaaca gaaacgccga cttgatctat gatctataca gcaacagaat
      301 gatccctctg aaacaaacag tcaataaata caatcggaca atagagatga tgcacgtaaa
      361 acttgatctg ataaataagg cgaagtctgt cttattagat acgatttatc tcgaatacag
      421 acccgccatc caaattgtta aaagtaaaga aatccccgca gatgcaagcg atacactcca
      481 atataaaatc ttaggagaac atatggccta ccgcaaggaa aaagactaca gcttaggaca
      541 tgcggtcgcc ggaatcgtag atatgaaaac agcgctgaat ccgaatatca ataaaactca
      601 atataaatat tactatacaa tccttccgtc agatcacgat gaaaaagatt atgacattat
      661 tgaaagcgga acttatatcg taatgtatca taaaggtccg tataaccaaa cttatcaagc
      721 ttatcctttt atgatagatt atgccggaaa gaaccatttt gaaatcgaat ctatgatcta
      781 cgacgaaagc ttaatagacg aaactacgga gtcagatccc gcaaactaca ttactcagat
      841 atccattaaa ataaaagaag taaaggggtc agattaagac ccttttactt tcttcatatg
      901 atgaaacaac ataatccgct tcgcttacgt cctgaccgtt ccctgaaccg gtaaatgccg
      961 cgacttcgat ccctgcctgt ttcgcggcgt gaatgccgac agacgaatct tcaaacacaa
     1021 gacactcctg cggcacagcg cctaataatc ccatcgcctt taaatagcat tccggatcag
     1081 gctttctctc ggcacacata tcaccgcaga cgatcacatc aaacaagtcc gtcatctgca
     1141 tccgattcag tcccataata ataagatggg ttgcagtaga agatacgagc gcggttttca
     1201 ctccgctttg tctcagccct tgtatcagcg gaacaattcc gggaatcgga gcaatatttc
     1261 cgttttcata tgtatttcca agttcttttc gtctccgtgt aagctgctct actgtcactt
     1321 tgcgaggcgc ccgcgacagg aattctataa gaagttcctg atcatgcgta ccgatcaacc
     1381 tctgcttgtc tttttccgtc agctcaactc cgaactcttt cagtgcaaga ccgagataat
     1441 gactgttgct ttcttccgta tctgcaatta caccgtcaaa atcaaataaa acatattttt
     1501 tcttcataac cgctcctttt ccgatccccg attgcttctg atctattttc cttactttcc
     1561 ctatggaata tcaggcgaat aaatgacgcg cttcttcctg gatttttttg atctctactt
     1621 ctgaaagatc gcaaaaatca ctgttttcca tagaac
//
LOCUS       DBMDXF010000299         1654 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_14545_length_1654_cov_1.000000, whole genome shotgun sequence.
ACCESSION   DBMDXF010000299 DBMDXF010000000
VERSION     DBMDXF010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14545_length_1654_cov_1.000000"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="ACZPD4_08925"
     CDS             complement(<1..527)
                     /locus_tag="ACZPD4_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HIR1379212.1"
                     /translation="MKGILDSWGVDPFYLFVVMFIIQIVMIVLYVMLNTKYRRLQKSY
                     VTFMRGKNGKNLEKSIFGKFDELDEIAELVRANEKEVKEISEKMKTHYQKVGIVKYDA
                     FHEMGGELSFALTMLDENNNGWIFNAMHSREGCYTYIKEVVNGESYIELSSEEKECLE
                     KAVYQEKYDLRDMEE"
     gene            complement(608..1522)
                     /locus_tag="ACZPD4_08930"
     CDS             complement(608..1522)
                     /locus_tag="ACZPD4_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1379213.1"
                     /translation="MAGKRNGLGKGLDSLIPNKTVKEESASQQKVSKEKQEKNVGSGE
                     ILVKINEVEPNREQPRKEFDEDSLMELADSIRQFGILQPLLVQKKKDYYEIIAGERRW
                     RAAKLAGIKDVPIIVKNYTDQEIVEISLIENIQRENLNPIEEAMAYKRLLEEFDLKQD
                     EVAERVSKSRTAVTNSMRLLKLSERVQQTIVDDMISTGHARALLALDDEEQQYILANK
                     IFDEKLSVRETEKLVKALKNPKKEVKVQKQEHMFVYDNLEEHLKNIIGSKVSVNPKAN
                     GKGKIEIEYYSEEELERIYDLIMSIQSK"
     gene            complement(1525..>1654)
                     /locus_tag="ACZPD4_08935"
     CDS             complement(1525..>1654)
                     /locus_tag="ACZPD4_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1379214.1"
                     /translation="PRNIRLAEAPSYGMPINLYDPKSTGATSYMLLAEEVIHKGEE"
BASE COUNT          410 a          386 c          193 g          665 t
ORIGIN      
        1 tattcttcca tatctctcaa atcatatttt tcttgataca ccgctttttc cagacattct
       61 ttttcttcgg atgataactc aatatagctt tcaccgttta caacttcttt tatataagtg
      121 taacagcctt ctctgctgtg cattgcattg aagatccatc cgttattgtt ttcatccagc
      181 attgtaagtg caaaactcaa ttctccgccc atttcgtgaa aagcatcgta tttgacaatt
      241 ccgaccttct gataatgtgt tttcattttt tccgaaattt cttttacttc tttttcattt
      301 gctctcacaa gttctgcgat ttcatccagt tcatcaaatt tgccgaaaat acttttttca
      361 agattttttc cgttttttcc acgcataaat gttacataac ttttctgtaa acgtctatat
      421 tttgtattca gcatcacata caatacgatc attacaatct gtatgataaa cataacgaca
      481 aacagataaa acggatctac tccccaacta tccaatatac ctttcatttc ttttcatctc
      541 cccatatttt atgacttttg cttcatcttt tttctatacg ttcttccttt tcacatttct
      601 atttcttcta ttttgattga atagacatga tcaaatcata gattctttca agttcttcct
      661 ctgaataata ttctatctca atctttccct ttccattcgc ttttgggtta acactcactt
      721 ttgatcctat tatatttttc aagtgttctt cgagattatc gtacacaaac atgtgttctt
      781 gtttttgtac ttttacttct tttttgggat tttttaaagc ttttacaagt ttttcagttt
      841 cccgaacgct taatttttca tcaaatatct tgtttgccaa tatatactgc tgttcttcat
      901 cgtcaagcgc caacaaagct cttgcatgtc ctgtactgat catatcatca actatcgtct
      961 gctgtacacg ctcacttagt ttaagcagcc gcatggaatt cgttaccgct gtccggcttt
     1021 ttgaaacccg ctctgcaact tcatcttgtt ttaaatcaaa ttcctctagc agacgtttat
     1081 atgccattgc ttcctcgatc ggattcaaat tttccctctg aatattttca ataagagaaa
     1141 tttcaacaat ctcctgatcc gtatagtttt tcacaataat cggaacatct ttaattcccg
     1201 caagctttgc agctctccat cttctctcac ccgctatgat ttcataataa tctttctttt
     1261 tttgaacaag aagcggctgt aatattccaa attgcctgat agaatctgcc aattccataa
     1321 gcgaatcttc atcaaattct tttcttggct gttctctgtt cggttcaact tcatttattt
     1381 ttactaatat ttccccggat ccaacatttt tttcttgttt ttctttactt actttctgct
     1441 gagatgctga ttcttccttc acagtcttat tcgggataag actatccaat ccttttccaa
     1501 gaccatttct tttccctgcc ataatcactc ttctccttta tgaataactt cttctgccaa
     1561 cagcatataa ctcgtagctc ctgttgactt cggatcataa agattgatcg gcattccata
     1621 actaggagct tccgctaatc taatatttct cgga
//
LOCUS       DBMDXF010000300         1645 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_14635_length_1645_cov_1.737694, whole genome shotgun sequence.
ACCESSION   DBMDXF010000300 DBMDXF010000000
VERSION     DBMDXF010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_1645_cov_1.737694"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..702)
                     /locus_tag="ACZPD4_08940"
     CDS             complement(127..702)
                     /locus_tag="ACZPD4_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1379215.1"
                     /translation="MEDKRIIRTKHNLKDAMIRMLSIEDFEHISVTELCKEAKVSRIT
                     FYSHYSDKQSLLDDIFNDMRSIGREDYYRRQKENNPEGELVLGYVNMLNAILDLYYAR
                     FDFFQYTNPEVNPYLASRLYSIILETVEMHTAHVRSKLKYSAKKIAGFVCFGMLGFVN
                     ECRREGNSLEQTKKETQCLLEEVLRSGILTE"
     gene            complement(763..1518)
                     /locus_tag="ACZPD4_08945"
     CDS             complement(763..1518)
                     /locus_tag="ACZPD4_08945"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR1379216.1"
                     /translation="MKLEENNIIVKRSYKTVYKCDDSIVKVFNEEHPKSDVFNEALIT
                     ARIEETGLDIPKVKGVSLVEGKWALEIEYKDGKTMEDMMNSDKKNIEKYMEDFVDLQL
                     QIHGKTSPLLKGMKDKLARQINSLKDLDATTRYELLTRLESMPKHNKVCHGDFNPSNV
                     IVGKNGKMTVVDWAHATQGNASADAAMTYLLFALKDQETADLYLKLFCKKSDTARQYV
                     QQWLPIVAAAQLTKDNELEKDFLMKWIDVIDYQ"
BASE COUNT          430 a          395 c          256 g          564 t
ORIGIN      
        1 agactcttcg actgtaatac aacaaaactg ccgctttaac gattcatgaa atcgtaaagc
       61 ggcagttcca tattctcatt ccgatgtatt cctacaattt cgcattttaa caaaaccgta
      121 tttaatctat tctgtcaaaa tccctgatct tagcacctct tccaacagac actgcgtttc
      181 ttttttcgtc tgctcaagag aatttccttc cctccggcat tcattcacaa atcctaacat
      241 tccgaaacag acaaaacctg ctatcttttt tgcggaatat ttcaatttac tccgcacatg
      301 agctgtatgc atctcaaccg tctctaaaat aatactgtaa aggcgagatg ccagatacgg
      361 attcacctcc ggattcgtat actgaaagaa atcaaaccgg gcataataaa gatccaaaat
      421 cgcattcagc atatttacat acccgagtac aagttccccc tccggattat tctccttctg
      481 ccgtctgtaa taatcttctc tgcctatact acgcatatcg ttaaagatat cgtccaaaag
      541 agattgcttg tcactgtaat gcgagtaaaa cgtaattctg ctgacctttg cctctttaca
      601 gagttccgta acagaaatgt gttcaaaatc ttcgatcgaa agcattctga tcatcgcatc
      661 cttaagatta tgctttgttc ttatgatccg cttatcttcc atatccggta aaattccttc
      721 ctctatccac tccttttact attgataata atatcgaaac ttctattgat agtcaataac
      781 atcgatccat ttcatcagga agtctttctc cagttcattg tcttttgtaa gctgcgctgc
      841 cgccacgatc ggaagccact gctgtacata ctgtcttgcc gtatcgctct ttttgcagaa
      901 caatttcaaa tacagatctg ctgtttcctg atctttcaat gcaaagagaa gatatgtcat
      961 agccgcgtcc gcacttgcat ttccctgtgt tgcatgcgcc cagtccacaa ccgtcatctt
     1021 gccgttttta cctacgatca cattactcgg attgaaatcg ccgtggcata ctttattatg
     1081 tttcggcata ctttcaagtc ttgtaagaag ctcgtatctt gttgttgcat ccaaatcttt
     1141 caggctgttg atctggcggg caagcttatc tttcattcct ttcagaagag gagatgtttt
     1201 gccgtgaatc tgaagctgaa ggtctacaaa atcttccata tacttctcaa tattcttttt
     1261 gtcggaattc atcatatctt ccattgtctt tccgtcttta tactcgatct cgagcgccca
     1321 cttgccttca accagggaaa ctcctttcac cttaggaata tccagtcctg tttcttcgat
     1381 ccgcgccgtg atcaaagctt cgttaaatac atctgacttc ggatgctctt cgttaaatac
     1441 tttcacaata ctgtcgtcac atttatagac ggttttatac gaacgtttta cgataatgtt
     1501 gttttcttca agtttcatgg taggttcctc cttaaatggg tttttgtttt tcgtttttga
     1561 tgagctcatt atacaccgta attttcagga aagaaacccc ttaaagcaaa ctttgtgcaa
     1621 tttttaacta tttttttatt attaa
//
LOCUS       DBMDXF010000301         1641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_14684_length_1641_cov_1.509740, whole genome shotgun sequence.
ACCESSION   DBMDXF010000301 DBMDXF010000000
VERSION     DBMDXF010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14684_length_1641_cov_1.509740"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="ACZPD4_08950"
     CDS             complement(<1..1400)
                     /locus_tag="ACZPD4_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1379217.1"
                     /translation="MIKSTAKKIYLFLIFVLLYAPIITLMVLSFNNTKTRSKWNGFTG
                     KWYLQLFQNKDIMNALYTTLIIAFLSALIATLIGTAAALGIQAMRTKARTLLLGVTNI
                     PMLNADIVTGISLMLLFIACRFTLGFSTILLAHITFNIPYVILSVMPKLKQTSKRTYE
                     AALDLGASPSYAFRKVVLPDIMPGIFSGFLLAFTMSLDDFVITHFTKGPGIDTLSTKI
                     YSEVRKGIKPEMYALSTIMFLTVLILLFLVNMTPDDPKVKKAAVHAPAGKFRKFSRFF
                     FHRFAPAAIMLVIVIGGFVYGSSSGNMSGEKVIVYNWGDYIDPEIITMFEDETGIDVV
                     YEEYETNEIMYPKVQSGAIAYDVVCPSDYMVQRMIENDLLAEINYENVPNLKYIDDIY
                     MEMSKQYDPENKYSVPYLWGTVGILYNKKMVDEPVDSWNILWDEKYTDSILMQDSVRD
                     AFAVALKSLGYSLNST"
     gene            complement(1397..>1641)
                     /locus_tag="ACZPD4_08955"
     CDS             complement(1397..>1641)
                     /locus_tag="ACZPD4_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1379218.1"
                     /translation="VPGIISGITMVFVPSLTTFVISDLLGGSKILLIGNVIEQEFKQG
                     SNWNVGSGLSLVLMLFIIASMALIAKYDKNGEGNTF"
BASE COUNT          487 a          324 c          353 g          477 t
ORIGIN      
        1 tctgttgagt tcaaagaata tccgagtgat tttaaagcaa cggcaaaagc gtctcttaca
       61 ctgtcttgca tgaggatgct gtccgtatac ttttcatccc ataaaatatt ccagctgtct
      121 acaggttcgt ccaccatttt cttgttataa agtattccga ccgttcccca aagatacgga
      181 acggagtatt tgttttccgg gtcatattgt tttgacattt ccatataaat atcatcaata
      241 tattttaaat tcggtacatt ttcataatta atctcggcaa gcagatcgtt ttcgatcatt
      301 ctttgcacca tatagtcaga aggacagacg acgtcataag caatcgcgcc ggactgcact
      361 ttcgggtaca tgatttcatt tgtttcatat tcttcatata caacgtcgat tccggtctcg
      421 tcttcaaaca tcgtaataat ttcaggatca atatagtctc cccagttgta tacaatgact
      481 ttttcgccgg acatatttcc ggaggaagaa ccatatacga acccgccgat aacgatgaca
      541 agcatgattg cggcaggagc gaaacgatga aagaaaaatc tgctgaattt gcggaatttt
      601 ccggcaggcg catgaacggc agcttttttg accttcggat cgtctggggt catattgaca
      661 agaaaaagca gaatgagaac cgtcagaaac atgattgtgg acagtgcgta catttccggt
      721 ttgattccct ttctgacttc actgtatatt tttgtagaca gtgtatctat tccgggtcct
      781 tttgtgaagt gggtgattac aaaatcatcc agcgacatag taaatgccag cagaaatcct
      841 gaaaaaattc cgggcatgat atccggcaga acgacctttc tgaacgcata agaaggagat
      901 gcgccgaggt caagcgccgc ttcataagtg cgtttgctcg tctgttttaa tttaggcatg
      961 acacttagga taacataggg gatattaaat gtaatatgtg ctagcaatat tgtggaaaat
     1021 ccaagtgtaa accgacatgc aatgaaaagg agcatgagag aaattcctgt tacaatatcg
     1081 gcgttgagca tcggaatatt tgtgactccg agaagtaatg tacgtgcttt tgtacgcatt
     1141 gcctggatgc cgagggcggc ggcagttccg atcaaagttg cgatcagagc ggaaagaaag
     1201 gcgatgataa gtgttgtata aagcgcattc atgatgtctt tgttttgaaa aagctgtaaa
     1261 taccattttc ctgtaaaacc gttccatttg gaacgtgttt ttgtattgtt aaaagaaaga
     1321 accatcaaag tgataatggg agcgtataac aatacaaaga tcaggaaaag gtagattttt
     1381 tttgcggttg atttgatcaa aatgtatttc cctccccgtt tttatcgtat ttggcaatca
     1441 gagccatgct tgcaatgatg aacaacatca aaacaagaga aagcccggaa ccgacattcc
     1501 agttgcttcc ttgtttaaat tcctgctcaa taacattacc gatcaggaga atcttacttc
     1561 cacctaagag gtcggagatg acaaaagttg tcagtgacgg tacaaagacc atggtaattc
     1621 cgctgatgat ccctggaacg c
//
LOCUS       DBMDXF010000302         1617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_14945_length_1617_cov_1.463061, whole genome shotgun sequence.
ACCESSION   DBMDXF010000302 DBMDXF010000000
VERSION     DBMDXF010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14945_length_1617_cov_1.463061"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            374..1525
                     /locus_tag="ACZPD4_08960"
                     /pseudo
     CDS             374..1525
                     /locus_tag="ACZPD4_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
BASE COUNT          499 a          270 c          389 g          459 t
ORIGIN      
        1 agtatgttaa tataggaaat aagatatttt cattttttat tcgtttagaa catgctgcca
       61 ggaaagtaaa agctttgatc gatagtttgt gaaaaaactg ttaatttaag aaaaggcgtt
      121 gacacatact tttatttcag atatattact gtttagtaaa agggagtaac tggcgcttgt
      181 taaaagcgtt tgcgatgtcg tcagtacgat gcatatgcat ccgtatcgtg attaaacagt
      241 gagactttta ttgcattaca taattagtgt gcaataggag tctctttttt attgtatgag
      301 aaatattact tcttatagga tgaaaaagct ccgtgcataa tgcacactca tataatctta
      361 aggaggaaaa cagatgaaaa cattttatca aatgtccgga gaggatacga tgaagcagtt
      421 aaacggctct gccgaaccgc ttactgaatc gcaggtgaaa gagcatcagg aaaaatacgg
      481 tcggaatgag cttgtggagg gcaagaaaaa gacggtgctt cagatctttc ttgaacagta
      541 caaggacttt cttgttatca tcctgatcat tgcggctatt gcatcgggat ttatgggaga
      601 tgtggaaagt acggcggtta ttttaatcgt aatcacaatg aatgcaatac tgggaacagt
      661 tcagactgtg aaagcagaac aatctcttgc cagcctgaaa aaactttccg gtccggaagc
      721 aaaagtgctg agaaacggaa cagttatccc gattccgtct tcggaagtaa cagtcggtga
      781 tattgtaatg ttggatgcag gagattacat tcctgccgac ggacgtcttt tggaaagcgc
      841 cagcctgaaa gttgatgaga gcgctttgac aggagaaagt cttggagtag aaaagtctgt
      901 agatattata aatgaagaag aggttccgct tggcgataga ttgaatatgg tttattcagg
      961 aagctttgtc acatatggaa ggggatcttt tgttgtaacc ggaatcggga tggagacaga
     1021 agtcggaaaa atcgcaagtc tgttaaagac aacttccgag aaaaagacgc cgcttcagat
     1081 caatctggat cagtttggtc aaaagttgtc gatcttgatc cttgtattct gtggtatttt
     1141 attcggaatc aatattttgc gaggcgataa gatcggtgat gcctttcttt ttgcagtagc
     1201 ccttgcagtg gcggcaattc cggaagcgct tagttctact gttacaattg tactttcttt
     1261 tggtacgcag aagatggcga aagaacatgc gatcatgcgt aaacttcagg cagtggaagg
     1321 actcggaagc gtatcgatca tttgttctga taaaacagga acgctgacac agaacaaaat
     1381 gacagtagaa gattattatg ttgatgagct ttgcattaaa gcagaagata tagatttgaa
     1441 tgacccttcc cagagcaggc tgttgatcgg aagtatgtta tgcaacgatt ctaaaaatac
     1501 agacggcgta gaaatcggag atcctcacag ctgtcagcca ggatatcctt gaaccaatac
     1561 agcgagctgg tattggccgc tttgcggacc aattccaaaa acagtttctt cgtcatg
//
LOCUS       DBMDXF010000303         1609 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15038_length_1609_cov_1.199602, whole genome shotgun sequence.
ACCESSION   DBMDXF010000303 DBMDXF010000000
VERSION     DBMDXF010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_1609_cov_1.199602"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(262..447)
                     /gene="rpmB"
                     /locus_tag="ACZPD4_08965"
     CDS             complement(262..447)
                     /gene="rpmB"
                     /locus_tag="ACZPD4_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR1379219.1"
                     /translation="MAKCAICEKGAHFGNNVSHSHRKTPKMWKSNVKSVRVKTEGGSK
                     KMYVCTSCLKSGRVERA"
     gene            634..993
                     /locus_tag="ACZPD4_08970"
     CDS             634..993
                     /locus_tag="ACZPD4_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1379220.1"
                     /translation="MKGSMSTDLGIITVNPEVIAKYAGSVAVECFGIVGMAAVNMKDG
                     LVRLLKKDSLTHGIQVTISDNNEITLNFHVIVAYGVNILSVSDNLMSNVKYKVEEFTG
                     MAVEKINIFVEGVRVID"
     gene            1007..>1609
                     /locus_tag="ACZPD4_08975"
     CDS             1007..>1609
                     /locus_tag="ACZPD4_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1379221.1"
                     /translation="MAVKTINTELFRKMFLAGAANLEAKKEFINELNVFPVPDGDTGT
                     NMTLTIMSAAKEVQSLENADMLTIAKAISSGSLRGARGNSGVILSQLLRGFTKEIREH
                     KEIDAVILAKACERATATAYKAVMKPKEGTILTVAKGISRKAEELAETTEDLEVFIPE
                     VIKYAEEVLAQTPEMLPVLKEAGVVDSGGQGLLEVIHGAYD"
BASE COUNT          503 a          250 c          375 g          481 t
ORIGIN      
        1 aaaaaaatat gtggctttgc attattttgg atggcgatcg gaatgtttat aatgatgatg
       61 cttccaaacg ttatttgggg agttattttg attattttgt ttctgcttgt cggatatcgg
      121 cttttttgct gttgttaaaa agaaaaataa agaagcggta ttatgttttt tcttgaaacg
      181 taaaaacaat gacataaaaa acggatctga atttacagat ccgctttttc cgtgtcattg
      241 tttccgatga tgtgtgtctg attaagcgcg ctcaacgcgt ccggacttca gacaagatgt
      301 acaaacatac atttttttgg aaccgccttc tgttttgact ctgacggatt ttacatttga
      361 tttccacatt ttcggtgttt ttctatgaga gtggcttaca ttgttaccaa aatgagcacc
      421 cttttcacaa atagcacatt tagccatgac tgcacctcct aacaatctga actcgtcata
      481 tagactcata acactgatat tctatcagaa acatttcagt attgcaagta ttttttaaaa
      541 aaaacgaatg tatattgtaa aatgttagaa gtgatagtat aataaacatg ttatttatgg
      601 caaaattgtg tgaaagaacc ggaggtatat attatgaaag gaagtatgag cacagatctt
      661 ggcattatca cggtcaatcc ggaagtgatc gccaaatatg cgggttccgt tgctgttgag
      721 tgtttcggaa ttgtcggaat ggcggctgta aatatgaagg acggacttgt gcgtcttctg
      781 aaaaaagata gtctgacaca tggaattcaa gtgacgattt ctgataataa tgaaattaca
      841 ttgaattttc atgtaatcgt tgcatatggc gtgaatatcc tttctgtttc tgacaatctt
      901 atgagcaatg taaagtataa ggttgaggag tttaccggaa tggcggtaga aaagatcaac
      961 atctttgttg aaggtgtaag agtgattgat taaggaggat attttcgtgg cagtgaaaac
     1021 gataaataca gagctgttcc gcaagatgtt tcttgcaggt gcggccaatc tggaagcaaa
     1081 aaaagaattt ataaatgaac tgaacgtatt cccggttccc gacggcgata cgggaacaaa
     1141 tatgacactt actattatgt ctgcggcaaa agaagtacaa tctcttgaaa atgcggatat
     1201 gttgacaatc gcaaaggcaa tttcttcagg atctcttaga ggagctcgcg gtaattcggg
     1261 agtaatttta tcacagctgc tcagagggtt tacaaaagag atcagagaac ataaagagat
     1321 cgatgcggtg attttggcaa aggcatgtga acgtgcaaca gcaacagctt ataaggcggt
     1381 tatgaaaccg aaagaaggaa cgatccttac tgttgcaaaa ggaatctcaa ggaaagcgga
     1441 agaacttgcg gagacgacgg aagatttgga agttttcatt cctgaagtga tcaaatatgc
     1501 ggaagaagtt ttggctcaga caccggagat gctcccggta ttaaaggaag cgggagttgt
     1561 agattccgga ggacagggat tacttgaagt gattcatggg gcgtatgat
//
LOCUS       DBMDXF010000304         1605 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15088_length_1605_cov_1.180851, whole genome shotgun sequence.
ACCESSION   DBMDXF010000304 DBMDXF010000000
VERSION     DBMDXF010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15088_length_1605_cov_1.180851"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1605)
                     /locus_tag="ACZPD4_08980"
     CDS             complement(<1..>1605)
                     /locus_tag="ACZPD4_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1379222.1"
                     /translation="YGEKIIFDDASFGIQKGDKIGIVGINGTGKSTLLKMIAGEETPD
                     KGQIIKQNGLKLAYVPQNPVFSEDSTIRSYALKKGEENWKVESNLTELNITRFDQKIE
                     HLSGGQKKRVVLAKILSDDFDVLLLDEPTNHLDEAMIRWLEEYLRSYKGTVIMVTHDR
                     YFLDRVTNRILEISHGKMYGYDSDYSGFLELKAQREEMESASERKRQSVLRMELEWAK
                     RGCRARTTKQRARLERLEQLKNGNTPIKDQTVELDSVETRMGKKTIELHHVSKKYGEK
                     VILDDFSYIVLKNQRLGIIGPNGCGKSTLMKMIAGQVIPDSGNIEIGETIKIGYFAQE
                     EQEMDPSQRVIDYVKDIAEYITTREGKISASALLERFLFTPDMQYAPIGKLSGGEKRR
                     LHLLGVLAENANVLLFDEAGNNLDIPTLTILEDYLNSFSGIVITVSHDRYFLDNIVDR
                     IFELDGKGHIRQFEGGYTDYLNVKKRESASTQFTDKTENSRGNLPKDPAESNPKSWKQ
                     NAPQKVKFSYKEQREFETIDDDIAALE"
BASE COUNT          395 a          394 c          253 g          563 t
ORIGIN      
        1 ttcaagcgct gcgatatcgt catcaatcgt ctcaaattct ctttgttctt tataagaaaa
       61 tttaaccttc tgcggcgcat tttgtttcca gcttttcgga ttactttccg caggatcttt
      121 cggaagattt ccgcgcgaat tttcggtttt gtctgtaaac tgcgttgatg cagattctct
      181 tttctttaca ttcaaataat cggtatatcc tccttcaaac tgacggatat gccccttccc
      241 atctaactcg aaaatgcgat ccacaatgtt atctaaaaaa tagcggtcat gcgatacggt
      301 gatcacgatt cccgaaaaag agtttaaata atcttccaaa atcgtaagtg tgggaatatc
      361 aagattattt cccgcctcgt caaacaaaag aacatttgca ttttcggcaa gtacgccaag
      421 aagatgcagc cttctcttct ctccgcccga aagtttacct atcggagcat actgcatatc
      481 aggagtaaac aaaaaacgct ccaacaacgc tgacgcactg atttttcctt ctcgtgtcgt
      541 aatatattct gcaatatctt tcacataatc gatcactctt tgagaaggat ccatctcttg
      601 ttcttcctgt gcaaaatatc cgatcttgat cgtttctccg atctcaatgt ttccactgtc
      661 cggaatcacc tgtcccgcga tcattttcat aagagttgat tttccgcatc catttggtcc
      721 tatgattcca agtctttgat tttttaacac aatataactg aaatcatcca gtatcacttt
      781 ttctccgtat tttttactca catgatgaag ttcgatcgtc tttttcccca ttctggtttc
      841 taccgaatca agttctactg tttgatcttt tatcggagta tttccgtttt tcagttgttc
      901 aagacgctcc agtctcgctc tttgtttcgt tgttctggca cgacacccgc gtttcgccca
      961 ttcaagttcc attctcaaaa cactctgtct ctttcgttca gatgctgatt ccatctcttc
     1021 tctttgcgcc ttcagttcaa gaaaccctga gtagtcagag tcgtatccat acatttttcc
     1081 atgactgatc tccaaaatac gatttgtaac tctgtcaaga aaataccggt cgtgagttac
     1141 cataataacg gttcctttat aggaccgaag atattcttcc agccatcgga tcatcgcttc
     1201 atccaaatgg ttcgtaggct catccagcaa aagtacgtca aaatcatctg acagtatttt
     1261 tgcaagcaca acccgttttt tctgtcctcc tgataaatgc tcgattttct gatcaaaccg
     1321 ggtgatattt aattccgtca gattactttc tactttccaa ttctcttccc ctttttttaa
     1381 tgcatatgac cgaatcgtac tgtcttctga aaaaaccgga ttttgcggca cataggcaag
     1441 cttcagtcca ttttgtttta taatctgtcc tttatccggt gtttcttctc ctgctatcat
     1501 tttaagaagc gttgattttc cggtaccgtt gatccctacg atcccgattt tatctccttt
     1561 ttgaattccg aatgacgcat cgtcaaatat tattttctca ccgta
//
LOCUS       DBMDXF010000305         1596 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15173_length_1596_cov_2.323746, whole genome shotgun sequence.
ACCESSION   DBMDXF010000305 DBMDXF010000000
VERSION     DBMDXF010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15173_length_1596_cov_2.323746"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(176..685)
                     /locus_tag="ACZPD4_08985"
     CDS             complement(176..685)
                     /locus_tag="ACZPD4_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR1379223.1"
                     /translation="MSISELTDYFLRYGAFFIYLIVLLEYLNLPGFPAGVIMPLAGIW
                     AAKGEISFPMVMVLTVFAGLTGSWALYLLGRFGGGKVMEFYFKKFPKHRPVIEEKMAF
                     LREKGGTGVFVSKLLPMVRTLISIPAGMVKMNFTKYTVSSACGIFLWNLAFVGAGYFF
                     GDAAINFFT"
     gene            complement(795..>1596)
                     /locus_tag="ACZPD4_08990"
     CDS             complement(795..>1596)
                     /locus_tag="ACZPD4_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DinB/UmuC family translesion DNA polymerase"
                     /protein_id="HIR1379224.1"
                     /translation="EGIKKKFGFTVNFGISTNKLLAKMASDFEKPDRIHTLFPEEIEV
                     KMWPLPIKDLYMAGKSSVQVLKKLEINTIGALAQADPDLISLHLKSHGRMLWEFANGI
                     GTSTVQSEPEEAKGIGNSTTLREDARTMEEVCPVFEELAQSVGRRLKKAGKKAGMVSA
                     EIKYYDFRTLSHQMQLEKPSNESDIICKAAVNLFREVWTGEPVRLLGIRTSKLSDENE
                     PEQLTIFDMKPEAEPDEKHKRLRKAMDELNKKYGEGAVIKASLMPKKK"
BASE COUNT          426 a          408 c          254 g          508 t
ORIGIN      
        1 gcactcctcc tacaaatggt ctgtaattgt ttgtcaacat tgcgattctc atgtcttttc
       61 ccccttgtct gaattctctc gccttatttt taataacttt ttcggataac aattattaaa
      121 taaactctca tttttcaaat gatataaaaa attatgtgaa aaattatgca taaatttatg
      181 taaagaaatt gatcgccgca tctccgaaga aatatcccgc gcctacaaac gccagattcc
      241 acaggaagat tccgcatgcg gagctaaccg tatattttgt aaaattcatt tttaccattc
      301 ctgccggaat agagatcagc gtgcgcacca tcggaagaag cttgcttaca aatacccctg
      361 ttcctccttt ttctcttaaa aacgccattt tttcttcaat caccggccga tgtttcggga
      421 atttcttaaa ataaaattcc atcaccttcc cgccgccgaa tcttccgaga agatacagcg
      481 cccagcttcc ggtaagtccc gcaaatacag tcagaaccat gaccatagga aaacttattt
      541 cacctttcgc cgcccatatt cccgccagcg gcataataac tcctgccgga aatccgggga
      601 gattcaaata ttctaaaagc acgatcaaat aaataaaaaa tgctccgtat cgtaaaaaat
      661 aatcggtaag ttcagatatg ctcataaaaa aatcagcctc ctttaactat ctacaggata
      721 tcctgtaaat ctaaaggggg ctgacataag attcaaaaga aattctaaag attttttgaa
      781 caagattttg atatttattt tttcttcggc ataagactcg ctttaataac tgctccttct
      841 ccgtattttt tattcagttc gtccatcgcc tttctaagcc gtttatgttt ttcgtccggc
      901 tctgcttccg gtttcatatc gaagatcgtc aactgctccg gttcgttttc atccgacaat
      961 tttgacgttc tgattcccag cagacgcacc ggttctccgg tccacacttc ccgaaacaga
     1021 ttgactgccg ccttgcatat tatatcgctt tcgtttgacg gcttttccaa ctgcatctga
     1081 tgagataatg tacgaaaatc atagtattta atttccgcac ttaccattcc tgcctttttc
     1141 ccggcttttt tcagacgtct tccgacgctt tgagccaatt cttcaaacac cggacaaact
     1201 tcctccattg tccgtgcatc ttccctaagc gttgtggaat tcccgatccc tttcgcttct
     1261 tccggctcag actgaacggt cgacgtaccg attccgttgg caaactccca caacatccgt
     1321 ccatgacttt tcaaatgaag tgagatcaga tcgggatctg cctgcgccaa tgctccgatt
     1381 gtattaatct ccagtttttt caatacctgc acactggatt ttcctgccat atacagatct
     1441 ttgatcggaa gcggccacat tttcacttca atctcttccg gaaataatgt gtgtatccgg
     1501 tccggctttt caaaatctga cgccattttc gccagtaatt tattcgttga gattccgaaa
     1561 tttaccgtaa atccaaactt ctttttaata ccttcc
//
LOCUS       DBMDXF010000306         1565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15583_length_1565_cov_1.155055, whole genome shotgun sequence.
ACCESSION   DBMDXF010000306 DBMDXF010000000
VERSION     DBMDXF010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15583_length_1565_cov_1.155055"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /gene="hisG"
                     /locus_tag="ACZPD4_08995"
     CDS             complement(<1..469)
                     /gene="hisG"
                     /locus_tag="ACZPD4_08995"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR1379225.1"
                     /translation="MRYLTFALTKGRLAGKTMKMLESLGITCEEMKEKDSRKLIFTNE
                     ELKLKFFLAKGPDVPTYVEYGAADIGVVGRDTIIEEGRKVHEVLDLGFGKCKMCVCGR
                     QETKELLNRHELIRVATKYPKIAKDYFYNKKHQTVELIKMNGSIELAPIVGLSE"
     gene            complement(497..>1565)
                     /gene="hisZ"
                     /locus_tag="ACZPD4_09000"
     CDS             complement(497..>1565)
                     /gene="hisZ"
                     /locus_tag="ACZPD4_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1379226.1"
                     /translation="STQELYKFFDREGNILALRPDITPSVARAAATLFDTEDFPLRFC
                     YVGNTFINHTSYRGKLKENTQFGAELIGLDSVEADAEMIAMVADALKKTGLEEFQISI
                     GHVDFLQSILSAAELDEETVERIRELIGNRNFFGAEELLEEKDVKKEIKEAFAILPEL
                     MGTEDVLDKAQQFAISDKAKAAIERLRQINHLLCLYNASDHVTFDLSMSGGYGYYTGI
                     VFRAYTYGTGDAVVRGGRYDHLLEKFGKETPSIGFAIIVDELMNALSRQKISVDTIRR
                     NIIVYNEETESNAIILAGNFREKGRCIELIRQKEGQDKSLYESYAKRKNAAALIYLCD
                     DKKLEMTNLITGEKKTANIAE"
BASE COUNT          406 a          379 c          258 g          522 t
ORIGIN      
        1 cctctgacaa accaacaatc ggagcaagtt cgatagaacc attcattttg atcagttcga
       61 ctgtctgatg cttcttatta taaaaataat cctttgcaat tttagggtat tttgtcgcaa
      121 cccggattaa ttcatgacga ttcagaagtt cttttgtctc ctgacgtccg cagacgcaca
      181 ttttgcattt tccgaaaccg agatctaaga cttcgtggac ttttcgtcct tcttcaataa
      241 tcgtatccct tccgacaact cctatatcag ccgctccgta ttctacatat gtcggtacgt
      301 ccgggccttt tgcaagaaag aatttcagtt ttaattcttc atttgtaaaa atcagttttc
      361 gtgaatcttt ttctttcatc tcctcacaag taataccaag actctcaagc attttcatcg
      421 tttttcctgc gagccttcct tttgtaaggg caaatgtcaa atatctcata ttctgctcct
      481 tttatactga cggcaattat tctgcaatgt ttgccgtttt cttttctcct gtaatcaaat
      541 tggtcatttc caacttttta tcgtcgcaaa gatagattaa agccgcagca ttttttcttt
      601 ttgcatatga ttcatacagc gatttatcct gtccttcttt ttgcctgatc aattcgatac
      661 agcgtccctt ttcccgaaaa ttaccggcaa ggatgatcgc attgctttca gtctcttcgt
      721 tatatacaat gatgttccgc cttatcgtgt ctaccgaaat tttctgtctt gaaagagcgt
      781 tcatcaattc atctacaatg atcgcaaatc caatagacgg agtctctttt ccgaattttt
      841 caagaagatg atcgtatctt ccgcctctta caaccgcatc acctgttccg tatgtatatg
      901 cccgaaatac gattccggtg taataaccat aacctccgct catactcaga tcaaaagtta
      961 catgatccga agcattatac aggcaaagaa gatgattgat ctgacgaagt cgttcaatgg
     1021 cggcttttgc tttgtcgctg attgcaaatt gctgcgcctt gtccagaaca tcctctgttc
     1081 ccattaattc cgggaggatc gcaaacgcct ctttgatttc tttttttaca tccttttctt
     1141 caagaagctc ttctgctccg aaaaagttgc gatttccgat caattcccgt atacgttcca
     1201 cagtttcctc gtctaactcc gctgcagata aaatactttg gagaaaatcg acatgaccga
     1261 tactaatctg aaattcttcc agtcccgtct ttttcaatgc gtctgcaacc attgctatca
     1321 tctccgcatc tgcctcaaca gaatcaagcc cgattagttc cgcgccgaat tgtgtatttt
     1381 cctttaactt gccgcgatag cttgtatgat tgataaaagt gtttccgaca taacaaaaac
     1441 gaagcggaaa atcttcagta tcaaaaagtg tagccgccgc ccttgcaacc gatggagtaa
     1501 tgtccggacg aagagccaat atatttcctt cacgatcgaa aaatttgtat aactcctgtg
     1561 tagaa
//
LOCUS       DBMDXF010000307         1558 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15673_length_1558_cov_1.729581, whole genome shotgun sequence.
ACCESSION   DBMDXF010000307 DBMDXF010000000
VERSION     DBMDXF010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15673_length_1558_cov_1.729581"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="ACZPD4_09005"
     CDS             <1..1007
                     /locus_tag="ACZPD4_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1379227.1"
                     /translation="HVTPIITYLNEGGTPAPEPTPELKAPVDVKVSEVTETSAKVSWK
                     NAADGKEATGYNVYVDGTKVNEELIAETTYNVSSLKDGTTYSVEVTAIDADGEESAKS
                     EKVEFTTVKKVVVDKEALKANIERASALLEETDKYTEESLKALEEALAAAQKVNSDPK
                     ADQTKVNDANTALEKAIKDLKEQEKPDPEPTPELKAPVDVKVSEITETSAKASWKNAA
                     DGKEAAGYNVYVDGVKLNKELLTEASCGLTNLKAETTYFVEVTAVDAAGNESVKSEKV
                     TFKTLKAEEQKEDSTPENNEKPGAVQTGDHANVFVWMIGLLISASAAVAVMFKRNKNR
                     "
     gene            complement(1105..>1558)
                     /locus_tag="ACZPD4_09010"
     CDS             complement(1105..>1558)
                     /locus_tag="ACZPD4_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1379228.1"
                     /translation="DKLISLYTDSDGLFPAANINISLINLMLYILDHTKPAHHFSNPQ
                     LQNSYVAYTLNYIAEHYTEKIIQEDIASQLHISVRYLSKLFKAHIGITLSSYINVYRI
                     NHSISLMQNTKLSLTEIALQSGFTNSQHYSKVFRDCVNTTPSQYRKSI"
BASE COUNT          536 a          268 c          392 g          362 t
ORIGIN      
        1 ttcatgtgac accgatcatt acttatctta acgaaggcgg aacacctgca cctgaaccga
       61 ctccggaatt gaaagctccg gttgacgtga aagtttcgga agttaccgag acaagcgcaa
      121 aagtatcatg gaagaatgcg gcggacggaa aagaagcgac aggatacaac gtatatgttg
      181 acggaacaaa agtgaatgaa gaacttattg cagagacaac atataacgta agcagcttga
      241 aagacggaac gacttactct gtagaagtga cagcgataga tgcagacgga gaagagtcgg
      301 caaaatcaga aaaagttgaa tttacaacag taaagaaagt tgttgtcgat aaagaagcgc
      361 tgaaggcgaa tattgaaaga gcatccgctc ttctggaaga gacagacaag tatacggaag
      421 agtctttaaa agcgcttgaa gaagctttgg cagccgcaca gaaagtaaac agcgatccga
      481 aagcagatca gacaaaagta aatgatgcga acacagcgct tgaaaaagcg atcaaagact
      541 tgaaagagca ggagaaaccg gatcccgaac cgactccgga attgaaagct ccggtcgacg
      601 taaaagtttc ggaaattacc gagacaagcg caaaagcatc atggaagaac gcggcggacg
      661 gaaaagaagc ggcaggatac aacgtatatg ttgacggtgt aaaattgaac aaagaacttc
      721 ttacagaagc atcttgcgga ttgacaaatc tgaaagcaga gactacatat tttgtagaag
      781 tgacggcggt agacgcagcc ggaaatgaat ctgttaaatc agaaaaagtg acgtttaaga
      841 cattgaaagc tgaagaacag aaggaagatt cgacaccgga aaataatgaa aaaccgggtg
      901 cggtacagac gggagatcat gcaaacgtat ttgtatggat gatcggactt cttatatcag
      961 cgtcggcggc tgtggctgta atgttcaaaa ggaataaaaa tcgttaaaaa gatatcatat
     1021 ttataatatg aatgcaggga gcggttaaaa atgaaccact ccctgttttg tatttcaaaa
     1081 catgcctttt gggcatatga ggatctatat agacttgcga tattgtgacg gcgttgtatt
     1141 tacacagtca cgaaatactt tagaatagtg ctgggaattt gtaaatccgg actgcagcgc
     1201 tatttcggtc agtgacagtt ttgtattttg catcaggctg atcgaatgat tgattcggta
     1261 tacgttaata tagctggaga gagtgattcc gatatgcgcc ttgaacagtt tgcttaaata
     1321 gcgaacagaa atatgaagct gtgacgcaat atcttcctgt atgatcttct cggtataatg
     1381 ctccgcaata taattcagcg tgtaagcaac ataactgttc tggagctgcg ggtttgaaaa
     1441 atgatgggcc ggttttgtgt gatccaatat gtacagcatt aagttgatca gagaaatgtt
     1501 aatattggct gccggaaaca gaccgtcaga gtcggtataa aggctgatca gcttgtct
//
LOCUS       DBMDXF010000308         1533 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15928_length_1533_cov_1.478352, whole genome shotgun sequence.
ACCESSION   DBMDXF010000308 DBMDXF010000000
VERSION     DBMDXF010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15928_length_1533_cov_1.478352"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="ACZPD4_09015"
     CDS             <1..376
                     /locus_tag="ACZPD4_09015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1379229.1"
                     /translation="IMPSVNKRDYFPVVSMTDGIVTSVGWLELGGYRVGITSPGGAYF
                     YYAHLSSYAGIKEGDPISAGDVIGFMGDTGYSKREGTTGKFPVHLHLGIYIYKDGKEI
                     SVNPYAALKYVENRKIKAYSDR"
     gene            478..1440
                     /locus_tag="ACZPD4_09020"
                     /pseudo
     CDS             478..1440
                     /locus_tag="ACZPD4_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
BASE COUNT          541 a          238 c          353 g          401 t
ORIGIN      
        1 tattatgccg tctgtcaata agcgggatta ctttcctgtc gtaagtatga ctgacgggat
       61 tgtaacgtcg gtcggttggt tagaattagg tgggtatcgt gttggaatca cttcaccggg
      121 aggcgcttac ttttattatg cacacttaag ttcttatgcc ggaataaaag aaggagatcc
      181 tatatcagcg ggcgatgtga tcggatttat gggagatacg ggatacagta aaagagaagg
      241 gacaacagga aagtttcctg tgcacttaca tcttggaatt tatatttata aagacggaaa
      301 agaaatcagt gtcaatccat atgcagcgtt aaaatatgtt gaaaacagaa aaatcaaagc
      361 atactcagac aggtagaata atcatcggaa agtcttttat gaagaggcaa aacatgatat
      421 gatagataac acatgaaaaa agagttgtga aaagcaacag aaaacagagg aaatattatg
      481 aatctaccga ttgaatttga agaaaaaatg caagatctgc ttggcgggga atataacgat
      541 tatttaaaat gttatgaaga accccgccat tatggactcc gtgtaaatac atccaaaata
      601 tcaatagatc gttttttaga gattgctcca tggccgcttc gtccgattcc gtggattgaa
      661 aacggttttt attatgatgg agaaaaatat cagcctgcaa agcacccgta ttattttgcg
      721 ggattgtact atttgcagga accaagcgca atgacgcctg caaacagact ccctatcgaa
      781 gccggagacc gagtgcttga tgtgtgcgca gcgccgggag gaaaagcgac agagttaggg
      841 gcaaagttaa acggatcggg agttttgatc gcgaacgacc tgagtaattc aagggcaaga
      901 ggacttttga aaaatattga acttttcgga atcggaaacg ttcttgtttt aagtgaagaa
      961 cccggaaagc ttgtgaatta ttttccggag tattttgata agatcttgat cgatgcgccg
     1021 tgctccggag aaggcatgtt tcgcaaggac agaaaaatgg taaaggcatg ggaagaacat
     1081 ggtccggaat tttttgtaaa aattcaaaga agcattatta cacaagcggc gcagatgctg
     1141 aaaccgggcg gaatgctact ttattctaca tgtacttttt caccggaaga aaatgaacag
     1201 acgattgaat ttctgttaca agaataccct caatttaaga tttgtgatat caaaggatac
     1261 gaagggtttt cagatgggat tttaaaagct tctttgagcg gcaatgaaca gttaaagaaa
     1321 acaatccgca tttttcctca taaaatggaa ggagaaggac attttatcgc gcttttaaaa
     1381 aaagaagaag aggcagtttc agaaaactta aaggaaaaag aaaaaaacaa tcgaaataaa
     1441 actttctaaa gaagataaat aacataaaaa ctgactttaa actattaggg attatgaaca
     1501 tgaaaaagaa aatgacagta catcggttat tgt
//
LOCUS       DBMDXF010000309         1529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_15975_length_1529_cov_1.347339, whole genome shotgun sequence.
ACCESSION   DBMDXF010000309 DBMDXF010000000
VERSION     DBMDXF010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15975_length_1529_cov_1.347339"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            31..870
                     /locus_tag="ACZPD4_09025"
     CDS             31..870
                     /locus_tag="ACZPD4_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR1379230.1"
                     /translation="MKRKVKTKVKGFRTTDGAGVKLVRVLGNLTTKEFDPILMLDSFD
                     SINPDDYTAGFPMHPHRGIETISYVSRGKMVHRDSLGNEAGISDGEVQWMNSGSGIMH
                     EEMVPAAERLLGVQLWLNLPAKDKMSKPSYNSIKNEDIKQIEIEGGKIRLLAGKYGEH
                     EGYKGDYLSMDYYDIHLNADAEVTLTMEEDDSVMVFTLLGDVYIDGEHIEEKTAVKLT
                     EGDSLTIKNGNEKAQVLYMNSRALNEPVAWAGPIVMNTQEELQKAFLELRSNTFIKET
                     AEY"
     gene            894..1460
                     /locus_tag="ACZPD4_09030"
     CDS             894..1460
                     /locus_tag="ACZPD4_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR1379231.1"
                     /translation="MSRGRMEAFSDGVLAIVITIMVLKLNAPKDLTLDAVKEVFPTFL
                     AYILSFIYVGIYWANHHHLINMTDSVSGRLLWKNLHWIFWMSLIPMATEWMGLNPYKS
                     LPALFYGIILFMCAVSYNIVQAEVIKINGKDSQIFRNIGNDLKGKLSIIAYAAAIIFA
                     FYFPVISYLIYIAIALTWIVPDSRLEKA"
BASE COUNT          527 a          211 c          362 g          429 t
ORIGIN      
        1 tcttttgaag aaagtaggag gaaaattata atgaaaagaa aagtgaagac taaagtcaaa
       61 ggattcagaa caacagatgg cgcaggcgta aaattagtaa gagttttggg gaatctgaca
      121 acaaaagagt ttgatcctat tttaatgctt gattcttttg atagcattaa tcccgacgat
      181 tatacagcag gatttccaat gcatccgcat agagggatcg aaacgatcag ttatgtatcg
      241 agaggtaaga tggttcatag agacagtctt gggaatgaag cgggtatttc agacggagaa
      301 gttcagtgga tgaattcggg ttccggaatt atgcatgagg agatggttcc ggcggcagaa
      361 aggcttttag gagtacagtt gtggctgaat ttaccggcaa aagataaaat gagtaagcca
      421 agttacaata gtattaaaaa cgaagatata aagcagattg aaatagaagg aggaaagata
      481 agacttttag ccggaaaata tggggaacat gaaggctata aaggcgatta tctctctatg
      541 gattattacg atatccatct aaatgctgat gcagaagtta cacttacgat ggaagaggat
      601 gattcggtaa tggtctttac acttttagga gacgtttata ttgacggcga gcacatagag
      661 gaaaaaacag ccgtcaaatt aacggaagga gacagtctta caattaagaa cgggaatgag
      721 aaggctcaag ttttatatat gaattcaagg gcgttgaatg aaccggttgc atgggccggt
      781 ccgattgtaa tgaatacgca ggaagagctt caaaaggcgt ttttagaact tagaagcaat
      841 acattcatca aagaaacagc agagtattaa tacgaactac aaggaaaagt ataatgagca
      901 gagggagaat ggaagccttt agtgatggcg tattagcgat cgttataaca atcatggtgc
      961 taaagcttaa tgcaccgaaa gatttaactt tggatgcggt aaaagaggtt ttcccgacat
     1021 ttttagcata tattctaagt tttatttatg tggggatata ttgggcaaac catcatcatt
     1081 taattaatat gacagattca gtatcaggta gattgttatg gaaaaattta cattggatct
     1141 tttggatgtc attgatcccg atggcaacgg aatggatggg actaaatccg tataaaagtt
     1201 taccggcttt attttatgga atcatactct ttatgtgtgc tgtctcttac aatatagtcc
     1261 aggctgaagt tataaagatt aatggaaagg actctcaaat tttccgaaat atcggaaatg
     1321 atttaaaagg aaagctttct attatcgcat atgccgccgc tattattttt gctttttatt
     1381 ttccggtcat ttcgtatttg atatatattg cgatcgcttt gacttggatc gtaccggaca
     1441 gcaggctgga aaaagcatga tgaaaaaggc gcaaacgtaa atgaaaaaat gtattgtata
     1501 agcaggaggt aatcgacagt gataaaaat
//
LOCUS       DBMDXF010000310         1526 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_16008_length_1526_cov_1.077193, whole genome shotgun sequence.
ACCESSION   DBMDXF010000310 DBMDXF010000000
VERSION     DBMDXF010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16008_length_1526_cov_1.077193"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1526
                     /gene="recG"
                     /locus_tag="ACZPD4_09035"
     CDS             <1..>1526
                     /gene="recG"
                     /locus_tag="ACZPD4_09035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1379232.1"
                     /translation="EEKRDTMQPVYSLTAGLTNNSVIKAVKQALSFLDDEQDILPEEL
                     RLKYSFPKYEEAIYAMHFPREKEDFVRARKRFVFEEFLLFILSLRRMKESENRSENPF
                     HFADRPEIEQFLGALPYHLTNAQEKVWTEIKSDMQSENVMSRLVQGDVGSGKTIIAFL
                     ALLLSALNGYQGALMAPTEVLAVQHYENISEMLEKYKIPLQAELLTGSMTMSQKKKAY
                     ARIESGEAAIIIGTHALIQEKVLYRNLALVVTDEQHRFGVRQRETLAQKGKTPHILVM
                     SATPIPRTLAIILYGDLDISIINEMPKNRLPIKNCVVNTEYREKAYRFMRKQVLEGRQ
                     CYVICPMVEESESLDAENVIDYSQMLAEELGDGIHVGCLHGKMKQKEKDEIMSAFGKN
                     EIQILVSTTVVEVGIDVPNATVMLIENAERFGLAQLHQLRGRVGRGGYQSYCIFMSAS
                     KSDETKERLEILNRSNDGFFIAGEDLRLRGPGDLFGIRQSGILDFKIADVFQDAEVLK
                     "
BASE COUNT          480 a          263 c          375 g          408 t
ORIGIN      
        1 gaggaaaaaa gagatacaat gcagccggta tatagcctga cagccggctt gacgaataat
       61 tctgtcatta aagctgtaaa acaagcactg tcttttttgg atgatgaaca ggatattctt
      121 ccggaagagc ttcggcttaa gtactctttt ccgaaatatg aagaagcaat ttacgcgatg
      181 cattttccga gagaaaaaga ggattttgtc cgggcacgaa aacggtttgt gtttgaagaa
      241 tttcttcttt ttattttatc actgcgtaga atgaaggagt ccgaaaatcg cagcgaaaat
      301 ccatttcatt ttgcagatcg tccggagata gaacaatttt taggagctct tccatatcat
      361 cttacaaatg ctcaggagaa agtttggacg gagattaaaa gtgacatgca aagtgaaaac
      421 gtcatgtcgc ggcttgttca aggagatgtc ggatccggaa aaacgatcat tgcatttttg
      481 gcgcttttgc tgtcagcatt aaacggatat caaggtgcgc ttatggcgcc gacggaagta
      541 ttggcggttc agcactatga aaacatatct gaaatgttgg aaaaatataa aatcccgctt
      601 caagcagaac ttttgacagg gtcaatgaca atgagtcaga aaaagaaagc ttatgcccga
      661 attgaatcag gagaagccgc tattataatc ggaacccacg cgcttataca ggagaaggtt
      721 ttatatcgta atcttgcgtt agttgttacg gatgagcagc atcgattcgg ggtaagacaa
      781 agggaaacac tggcgcaaaa agggaagaca cctcatattc ttgtaatgag cgcgactccg
      841 atcccgcgga cgcttgcaat tatcttatat ggcgatcttg acatttctat tatcaatgaa
      901 atgccaaaaa acagattgcc gataaaaaat tgtgtcgtaa atactgaata tcgggaaaaa
      961 gcatatcgtt ttatgagaaa acaggtttta gagggacgac aatgctatgt gatctgtccg
     1021 atggtagagg aaagtgaaag tctggatgcc gaaaatgtta tcgactactc gcaaatgctc
     1081 gcagaagaat tgggggatgg tattcatgta ggctgccttc acggaaaaat gaagcagaag
     1141 gaaaaagatg agataatgtc tgcatttggg aaaaatgaaa ttcagatact tgtatcgaca
     1201 actgttgttg aagttgggat cgatgtgcca aatgcaacag ttatgcttat tgaaaatgcg
     1261 gagcgtttcg gacttgccca acttcatcag cttcgcgggc gtgtcggaag aggcggatat
     1321 caatcttatt gtatttttat gtctgcgtct aaatcggacg agacaaaaga aagacttgaa
     1381 atcttgaacc gctcgaacga cggtttcttt atcgccgggg aagatctgcg tcttagagga
     1441 ccgggagatc tgttcggaat acgtcaaagc gggattcttg actttaagat tgcagatgtg
     1501 tttcaggatg cagaagtgtt aaaaat
//
LOCUS       DBMDXF010000311         1494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_16421_length_1494_cov_1.562814, whole genome shotgun sequence.
ACCESSION   DBMDXF010000311 DBMDXF010000000
VERSION     DBMDXF010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16421_length_1494_cov_1.562814"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="ACZPD4_09040"
     CDS             <1..238
                     /locus_tag="ACZPD4_09040"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1379233.1"
                     /translation="PLVLHGASGIGDEDIKKAISLGIAKINIHTELCQAAMEAINEHK
                     DEPFLAVERAVRQAVKERAMYKIKLFGDDGRADE"
     gene            231..1199
                     /locus_tag="ACZPD4_09045"
     CDS             231..1199
                     /locus_tag="ACZPD4_09045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1379234.1"
                     /translation="MNKVSKDLDVMIIGAAIVDLPLRPVSKEVFDVVSYPVDGIAMTI
                     GGDALNESTIITRLGHKVALMSCIGVDVAGAFVLSHCERTGIDTKYIKQDPAIDTSIN
                     VGLVAADGERTFITNRQGSLWKFKYEDLDMSALKGAKILSFASIFNNPLFDNKALVSV
                     FSKAKEEGMIICADMVSPRLNEKLEDITEALSYVDYFFPNFEEASEMTGETDEAKVAD
                     KLYACGVKNVIMKIGKRGCYIRNADGAMIVPACKGVTAIDTIGAGDNFASGFITGLLE
                     GKDIRECAIYANCTAAVSVQYVGATGGVTDKAIVDKMLAKYREDYK"
     gene            1263..>1494
                     /locus_tag="ACZPD4_09050"
     CDS             1263..>1494
                     /locus_tag="ACZPD4_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1379235.1"
                     /translation="MKTMKLGKTGIDISRIGLGTWSIGGGSAWGGDHELQTVVDTIRE
                     APKAGVNLIDTAPGYNFGNSEKILGKALEKMNR"
BASE COUNT          475 a          236 c          405 g          378 t
ORIGIN      
        1 tccgcttgta cttcacggag catcaggtat cggagatgaa gatattaaga aagcgatctc
       61 tcttggaatc gctaagatca atatacatac agaattatgc caggctgcta tggaagcgat
      121 caacgaacat aaagacgagc cgttcttggc tgtggaacgt gcggtaagac aggctgtaaa
      181 agaacgtgca atgtacaaga tcaaattatt tggtgatgac ggaagggctg atgaataagg
      241 tgagtaaaga tttggacgta atgattatcg gggcggcgat cgtagatctg ccgctgcgcc
      301 cggtaagtaa agaagtgttt gacgttgtat cttatccggt agacggaatt gcaatgacga
      361 tcggaggaga tgcgcttaat gaatcgacca ttattacaag actgggacat aaagttgctt
      421 tgatgagctg tatcggtgtc gatgtagccg gagcgtttgt gttaagtcat tgtgaaagaa
      481 caggaatcga tacaaaatat atcaaacaag atccggcaat cgatacttcg atcaatgtcg
      541 gtctggtagc ggctgacgga gagagaactt ttatcacgaa ccgtcaggga agtctttgga
      601 aatttaagta cgaagatttg gatatgtcag ctttaaaagg agcaaagatt ctttcgtttg
      661 caagtatttt caacaatccg ctttttgata ataaagcgct tgtatctgta ttttccaaag
      721 caaaagaaga aggtatgatc atttgtgccg atatggtgtc gccaagattg aatgagaaac
      781 tggaagatat tacggaagcg ttgagttatg tagactattt cttcccgaat tttgaagagg
      841 caagtgaaat gacgggagag acagacgaag cgaaagtggc tgataagtta tatgcctgcg
      901 gtgtgaaaaa cgtaatcatg aagatcggta aaagaggatg ctacatccgt aatgcagacg
      961 gtgcgatgat cgttccggca tgcaaaggag tgacggcaat cgatacgatc ggagcaggag
     1021 ataatttcgc atccggattt attacaggcc ttttggaagg aaaagatatc cgtgaatgtg
     1081 cgatctatgc aaactgtact gcagctgttt ctgttcagta tgtcggcgcg acaggcggtg
     1141 tgacagataa agcgatcgta gataagatgc ttgcgaaata ccgtgaagat tataaataat
     1201 atctgcatac ttaaaagtgt ttggttggat gaaataggtt aagaaaggga tagacagcta
     1261 ttatgaaaac gatgaaatta ggaaagacag gaatagatat ttcaagaatc ggacttggaa
     1321 catggtcgat cggaggcggt tctgcgtggg gcggagatca tgagcttcag acagttgtag
     1381 atacgataag ggaagctcct aaggcaggag tcaatctgat cgatacagcg ccgggataca
     1441 atttcggaaa cagtgaaaaa attctcggaa aagctcttga gaaaatgaat cgcg
//
LOCUS       DBMDXF010000312         1475 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_16649_length_1475_cov_1.743814, whole genome shotgun sequence.
ACCESSION   DBMDXF010000312 DBMDXF010000000
VERSION     DBMDXF010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16649_length_1475_cov_1.743814"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="ACZPD4_09055"
     CDS             <1..307
                     /locus_tag="ACZPD4_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1379236.1"
                     /translation="KKIKMINPFGEYLFELNGKRIRTYSFTSRLKSICKKLDISPKSL
                     NKIRKTYATTLIDSGVEESLIISQMGHTDIDTTKKYYYKNRKNLEQKDKAIDMVSNL"
     gene            complement(441..1340)
                     /locus_tag="ACZPD4_09060"
     CDS             complement(441..1340)
                     /locus_tag="ACZPD4_09060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1379237.1"
                     /translation="MMYDVVIIGAGPAGISAGIYAASRGKKVLITEKNTVGGLIGKVS
                     TVTHYAGIMTEETGSSFAKRLKIQAENTGIDIIYEEVTQVELQGSIKKVFTKDGAYDA
                     KKIVLANGTTPRKLNIPGEAELTGKGMGMNAQRDGRKYTDKNIYVVGGADGAVKEALY
                     LSKFAKQVTIIHFEDTLGCIEEFKNKAEQTPNIKLRLGSRLHAVYGAEQVEALEISDE
                     KTGSVEKIADCGCGIFVYAGTTPNTELYSELELDNGFIPVDENMETNIAGVYAVGDIR
                     VKQVRQVSTAVADGTVAGIHLAK"
BASE COUNT          419 a          296 c          244 g          516 t
ORIGIN      
        1 taaaaaaata aaaatgataa atccttttgg ggaatatcta tttgaattaa acgggaaaag
       61 aataagaaca tatagtttta catcaagatt aaagtcaata tgcaaaaagc tagatattag
      121 tccgaagtct ttaaataaaa taagaaaaac ttatgccacc actttaatag acagtggagt
      181 agaagagtct ttgattatat ctcaaatggg acatacagat attgatacaa caaaaaaata
      241 ttattacaaa aacagaaaaa atctggaaca aaaagacaag gcaatagata tggtttctaa
      301 tctataaagt aatcaaaaag taatcaaagg taatcataat aaaaactgaa aaacccttat
      361 ttttcaatga attttgaggt tttgaaaggg gttcgattcc cctcggagtc atttggaaag
      421 tccgtattta cggacttttt ttattttgcc aaatggattc ccgcgactgt tccgtctgcg
      481 actgccgttg atacttgacg tacttgtttt actcggatat ctccgactgc ataaactcct
      541 gctatgtttg tttccatatt ttcatcaacc gggataaatc cgttatctaa ttctaattct
      601 gaatataact ctgtattcgg tgtcgttccg gcgtagacaa agataccgca tccgcagtct
      661 gcgatctttt cgacagaacc tgtcttttca tcggaaattt ccaacgcttc tacctgttct
      721 gctccatata cggcgtgaag tcttgatcct aaacgcagtt ttatgtttgg agtttgttct
      781 gctttgtttt taaactcctc aatgcagccg agtgtatctt caaaatgaat gatcgtaact
      841 tgtttcgcaa attttgataa atacagagct tcctttacag ctccgtccgc gcctcctacg
      901 acgtaaatgt ttttgtctgt atattttctt ccgtctcttt gagcgttcat tcccatccct
      961 ttgccggtaa gttctgcttc tccggggatg ttgagttttc tgggggttgt accgtttgcc
     1021 agaacgattt tttttgcatc ataggcgccg tcttttgtga acactttttt aatgctgccc
     1081 tgcagctcaa cttgcgtcac ttcttcgtat atgatatcaa tacctgtatt ttctgcctgg
     1141 attttcagtc tcttggcaaa agaacttccg gtttcttctg tcataattcc cgcataatgg
     1201 gtaacggtag atacttttcc gatcaatcct cccacggtgt ttttttctgt gatcaacact
     1261 ttttttcctc tgcttgccgc ataaattccc gcgctgatcc ctgccggtcc tgctccgatg
     1321 atcactacgt catacatcat aacttcatcc tcttttcttt cataaattgc attttaaagt
     1381 gttcccttaa ttcatactat acacttggat agttattttg taaaattata ataaactata
     1441 tattcataaa tatttattat gataataggc attat
//
LOCUS       DBMDXF010000313         1456 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_16872_length_1456_cov_1.589668, whole genome shotgun sequence.
ACCESSION   DBMDXF010000313 DBMDXF010000000
VERSION     DBMDXF010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16872_length_1456_cov_1.589668"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1456)
                     /locus_tag="ACZPD4_09065"
     CDS             complement(<1..>1456)
                     /locus_tag="ACZPD4_09065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1379238.1"
                     /translation="LITLGLYVVSALCSFIQGMIMTGISQKTTYRLRKDITEKVNRMP
                     MDYFDTKPVGEVLSRVTNDVDTLGQSLNQSATQLITSVTTLIGVLVMMLSISPLMTVV
                     ALLILPISVGLISFVMKHSQKYFSGQQEYLGNVNGQVEEVYSGHNVIKAFNKEDDVIR
                     EFDRTNDKLYESAWKSQFFSGMMMPVMQFVGNLGYVGVAILGGFLAIKKTIEVGDIQS
                     FIQYVRNFTQPIQQVAQVTNMLQLAAASSERVFEFLEEKEEDQTVEHPVSVEGLHGNV
                     QFENVHFGYNPEKIIINDFSANVKEGQKIAIVGPTGAGKTTMIKLLMRFYDVNSGSIK
                     IDGHDVRDFNRSELREMFGMVLQDTWLFHGTIMDNIRYGKLDATEEEVIQAAKAAHVH
                     RFVQTLPGGYQMEINEEATNISQGQKQLLTIARAILADPKILILDEATSSVDTRTEVR
                     IQKAMDNLMKGRTSFVIAHRLSTIRDADLILVMK"
BASE COUNT          359 a          346 c          290 g          461 t
ORIGIN      
        1 tctttcataa cgagaatgag atctgcgtct cggattgtcg ataatctatg ggcgataacg
       61 aaacttgttc tgcctttcat caaattatcc attgcttttt ggattcttac ttctgttctt
      121 gtatcgacgg aacttgtcgc ttcgtcaaga atgaggatct tcggatctgc aagtattgct
      181 cttgcgatcg taagaagctg tttttgtcct tgtgagatgt ttgttgcttc ttcattgatt
      241 tccatctgat agcctcccgg aagtgtctgg acgaaacggt gaacatgtgc tgcttttgct
      301 gcctgtataa cttcttcctc tgttgcgtcg agttttccgt aacggatgtt atccatgatc
      361 gttccgtgga agagccatgt atcttgaagt accattccga acatttctct tagttcgctt
      421 ctgttgaagt ctcttacgtc atggccgtcg atcttgatcg aaccgctgtt tacatcataa
      481 aagcgcatga gcagtttgat catcgttgtt tttccggctc ctgtaggacc gacgattgcg
      541 attttctgac cttcttttac atttgcagag aaatcgttaa tgatgatctt ttccggattg
      601 tatccgaaat gaacattttc aaactgtaca tttccgtgaa gtccttctac cgaaacaggg
      661 tgttcaaccg tctgatcttc ttccttttct tcgaggaatt caaacacccg ctccgatgat
      721 gccgccgcaa gctgaagcat gtttgtcacc tgagcaactt gttggatcgg ctgtgtaaag
      781 tttctcacat actgaataaa tgactggata tctccgactt ctattgtctt tttaatcgcc
      841 agaaaacctc cgagtatcgc tactcctaca taacctaagt tccctacaaa ctgcatgacc
      901 ggcatcatca ttccagagaa aaactgggat ttccatgcag attcatacaa tttatcattt
      961 gtccggtcaa attcacggat cacatcgtct tctttgttaa atgccttgat cacgttgtgt
     1021 ccgctgtaca cttcctcgac ctgaccgttt acatttccga gatattcctg ctgtccgctg
     1081 aaatatttct gcgaatgttt cataacgaat gagatcaggc caacggaaat cggcaggatc
     1141 aacagcgcca caaccgtcat aagcggactg atcgaaagca tcataacgag aactcctatc
     1201 agagtcgtca cagaagtaat aagctgcgtc gcgctttggt tcaaactctg tccgagagta
     1261 tctacatcat ttgtcacacg ggagagcacc tctccgacag gcttcgtatc aaaataatcc
     1321 atcggcatcc gattgacctt ttccgtaata tcttttctca atcggtacgt cgttttctgt
     1381 gagattccgg tcataatcat tccctgaata aaggaacaaa gcgcgctgac tacatacaga
     1441 ccgagagtaa taagaa
//
LOCUS       DBMDXF010000314         1447 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_16983_length_1447_cov_2.154532, whole genome shotgun sequence.
ACCESSION   DBMDXF010000314 DBMDXF010000000
VERSION     DBMDXF010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16983_length_1447_cov_2.154532"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="ACZPD4_09070"
     CDS             <1..687
                     /locus_tag="ACZPD4_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR1379239.1"
                     /translation="AVNGFLKEGKEMYKAKEAYQSIDKTDENADTLKDLLDKNPETWE
                     FVKNYSKMKDASPADTIGEVAAGEIPLLLQWDERWGYSVYGDNMIAVNGCGPTAVAMV
                     VSGLLCDPSVTPYKVAKFAEEQGYYAGESGTSWTLMSEGVQSFGIWGEELGLSRSEVF
                     SALESGCPIICSMRPGDFTTTGHFIVLTKVENGKIKVNDPNSRIRSGQLWDYERLEPQ
                     IQNLWAFSRM"
     gene            complement(784..>1447)
                     /locus_tag="ACZPD4_09075"
     CDS             complement(784..>1447)
                     /locus_tag="ACZPD4_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine nucleotide alpha hydrolase family
                     protein"
                     /protein_id="HIR1379240.1"
                     /translation="ISHEGLELEDPANEPNYDDLLVLGVTPEKAPDEGEYVTMTFEAG
                     VPKTLNGKEMKVSEIITELNALGGKHGVGIVDIVENRVVGMKSRGVYETPGGTILMAA
                     HEQLEELVLDRATYEVKKDLGNKFAQIVYEGKWFTPLREAIQAFIDVTQQYVTGEVKF
                     KLYKGNIIKAGETSPYSLYSESLASFTTGELYDHHDAEGFINLFGLPLKVRAMKMQEL
                     EK"
BASE COUNT          395 a          279 c          362 g          411 t
ORIGIN      
        1 gcagtcaatg gatttctgaa agaaggaaaa gaaatgtata aagcaaaaga ggcttatcag
       61 tctattgaca aaaccgatga gaatgcggat actctcaaag atctccttga taaaaatcct
      121 gaaacttggg aatttgtaaa aaattattct aaaatgaaag atgcttctcc ggcagatacg
      181 atcggggaag tggctgcagg agagattccg ctcctgttac agtgggatga aagatgggga
      241 tattctgttt atggagataa tatgatcgca gtcaacggat gtggaccgac ggcggtcgct
      301 atggttgttt ccggactgct ttgtgatccg tccgtcacac cgtataaggt ggcaaagttt
      361 gcagaggaac aaggatatta tgcgggagag tcgggaacaa gctggacgct tatgtcggaa
      421 ggtgttcagt cgttcggaat atggggcgaa gagcttggtc taagccggtc ggaagtattt
      481 tcggcattgg aaagcggctg tccgatcata tgtagtatgc ggcccggaga ctttacgacg
      541 acaggacact ttattgtgct tacgaaggtc gaaaacggaa aaataaaagt gaatgatccg
      601 aacagcagga tcagaagcgg gcagctatgg gattatgaaa gactggaacc gcagattcag
      661 aacttatggg cattctcgag gatgtagaga gaatgaatag aaagacagcg ttttgctttg
      721 aaaatattaa agcaagacgc tgtccgattt tatgacataa acaaattgtc gtatggataa
      781 attttatttc tcgagttcct gcattttcat tgcacggact tttaacggaa gaccgaacag
      841 gttgataaat ccttctgcat cgtggtgatc gtacaattcg ccagttgtaa agcttgcgag
      901 ggattcgctg tacagagagt acggagatgt ttcgcctgct ttgatgatgt ttcctttata
      961 taatttgaat tttacttcac ctgtcacata ttgctgtgta acatcgataa atgcctgaat
     1021 tgcttcacga agcggtgtaa accattttcc ttcgtataca atctgtgcaa atttattgcc
     1081 gagatctttt ttgacttcgt atgtagcacg atcgagaaca agttcttcga gttgttcgtg
     1141 agcagccata aggatcgttc cgcccggtgt ctcgtatacg ccgcgtgatt tcattccgac
     1201 aacgcggttc tcgacaatat caacgatgcc gacaccgtgt tttccgccaa gtgcattcag
     1261 ttcggtaatg atttcggaaa ctttcatttc ctttccgttc agagttttcg gaacgccggc
     1321 ttcaaatgtc attgtcacgt actcgccttc gtcaggagct ttttccggag ttacgccaag
     1381 aacgagcaga tcatcgtaat tcggttcgtt tgcaggatct tctaattcca gtccttcgtg
     1441 gctgatg
//
LOCUS       DBMDXF010000315         1416 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_17419_length_1416_cov_2.496578, whole genome shotgun sequence.
ACCESSION   DBMDXF010000315 DBMDXF010000000
VERSION     DBMDXF010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17419_length_1416_cov_2.496578"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(286..>1416)
                     /locus_tag="ACZPD4_09080"
     CDS             complement(286..>1416)
                     /locus_tag="ACZPD4_09080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1379241.1"
                     /translation="VDVTKMPKLGFGMMRLPEKDGELDLEQICKMVDAYLASGMNYFD
                     TAYMYCGGKSESIVKKALVERHPRESFTLTTKLPQWMMDEGIEGRDRIFNDQLARTGA
                     GYFDYYLLHSVEDGANYEGYVKYDCFNWAKKKKEEGLIKHFGFSFHGTPELLDKILSE
                     HPEVEIVQIQMNYADWDNPLIQSGRLYEVLRKYNMPFLVMEPVKGGSLASAGKEIEAE
                     MKRVHPDASIASWALRFAASLPGVATVLSGMSNEEQMEDNIKTFRNFVPLNEEEKAVI
                     AKAQEALKKSPAVPCTACRYCCDGCPMGIAIPDVFKALNTIRLYGEEDRAKNYYKKLL
                     ETSGKAEECLQCGQCESVCPQHLPIIDLLKEASEKLDVPMTE"
BASE COUNT          328 a          375 c          302 g          411 t
ORIGIN      
        1 ggcgtgtaaa atgtaaaatc tcgaagaggt taagtttatg taaaataaaa atttttccat
       61 tttacattcc attttttccg gtcgtaaacc gctccgtcat aactatcgca gatggcacaa
      121 ttttttccgc gcagtgtgat cctgcccgaa gggtcctttt tctcctcaga aactttcaac
      181 aagatttccc atagaaaaaa aggatccccg gaatactctt ttttcagagt attccgggga
      241 tccttttttc taaagcatgt gccaaagcgg atattcgcaa tccgcttatt ccgtcatcgg
      301 cacgtcaagt ttttcggatg cctcttttaa cagatcaatg atcggcagat gctgcggaca
      361 tacgctctcg cactggccgc actgcagaca ttcttctgct tttcccgatg tttccagcag
      421 ctttttataa taatttttcg cgcggtcttc ttctccatac aggcggatgg tattcaatgc
      481 cttgaataca tccggaatcg cgattcccat cggacatccg tcgcagcagt aacgacaggc
      541 ggtacatgga accgccggac tctttttcag cgcttcctgt gctttggcga tcactgcttt
      601 ttcttcctcg tttagcggta caaaatttcg gaaggttttg atattatcct ccatctgttc
      661 ctcgtttgac ataccggaga gcacggttgc aacacccggg agggaagccg caaagcggag
      721 tgcccaggac gcaatcgatg catccggatg aacacgtttc atctctgctt caatctcttt
      781 gcctgccgat gcaagggaac cgccctttac cggctccata acaaggaatg gcatattgta
      841 tttccgaagc acctcataca gacgtccgga ctggatcagc ggattgtccc agtcagcata
      901 gttcatctgg atctggacaa tttcgacctc cggatgctcg gagagaatct tgtcaagcag
      961 ctccggcgtt ccgtggaagg agaaaccaaa gtgtttgatc agtccctctt cttttttctt
     1021 cttggcccag ttgaaacagt cgtatttcac atagccttca tagtttgcgc cgtcttctac
     1081 gctatgcagc aggtaataat caaagtagcc tgctccggtg cgcgcgagct gatcgttgaa
     1141 aatgcggtca cgcccctcga tgccctcgtc catcatccac tgcggaagtt tggttgtcag
     1201 agtaaaactt tctctcggat gacgctctac cagcgctttt ttcacgatac tctccgattt
     1261 tccgccgcag tacatatagg cggtgtcaaa atagttcatt ccggaggcaa ggtacgcatc
     1321 aaccatttta cagatctgct ccaaatccag ttctccgtcc ttctccggaa ggcgcatcat
     1381 gccaaatcct aatttcggca tttttgttac atctac
//
LOCUS       DBMDXF010000316         1383 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_17910_length_1383_cov_1.344774, whole genome shotgun sequence.
ACCESSION   DBMDXF010000316 DBMDXF010000000
VERSION     DBMDXF010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17910_length_1383_cov_1.344774"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="ACZPD4_09085"
     CDS             <1..698
                     /locus_tag="ACZPD4_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1379242.1"
                     /translation="MREIMNILIGLIVSVYVLFSKEKFSKQSKKITYAIFKPSNANMI
                     LHLTIKSNEIFGGFIIGKIIDSAIIGVLCFAGLSILNMPYAMLVSVIVGVTNVIPFFG
                     PYIGAIPSAILILLAEPKMGIYFIIFIIALQQFDGNVLGPKILGDSTGLSAFWVVFSI
                     LIGGGLFGVPGMILGVPTFAVVYYIVGMLVNNKLEKKKLPVKTDAYDEYSYVESDGTY
                     VHADKNIFKEKER"
     gene            703..>1383
                     /locus_tag="ACZPD4_09090"
     CDS             703..>1383
                     /locus_tag="ACZPD4_09090"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HIR1379243.1"
                     /translation="MPIRVQNDLPVKEILEHENIFVMDEYRAAHQDIRPISIGLLNLM
                     PLKEDTELQILRSLSNTPLQVDVTFVRMTSHVSKNTSTSHIYKFYESFEDIKMRKFDG
                     FIITGAPVEQMEFEEVDYWQELTEIMEWTKTNVTSTLHLCWGAQAGIYYHYGINKKAL
                     PKKLSGVYRHRVRNRKIPLVRGFDDVFMAPHSRHTEVPQELLEKDDRIMILADSLQAG
                     VFMCMAKEG"
BASE COUNT          429 a          211 c          305 g          438 t
ORIGIN      
        1 tcatgcgtga aattatgaat attttgatcg gattaattgt ttctgtctat gtactgttca
       61 gtaaagaaaa gttttcaaaa cagtctaaaa aaataacata tgctatattt aaaccgtcaa
      121 atgccaatat gattcttcat cttactatta agagtaatga aatattcgga ggatttatta
      181 taggaaaaat cattgattcg gctattatcg gtgttctttg tttcgcaggc ctgtcgattt
      241 tgaatatgcc atatgcgatg cttgtcagtg tgatcgtcgg tgtgacaaat gtaattccgt
      301 ttttcggacc ttatataggg gcaatcccaa gcgcgattct tattttgctt gcagagccta
      361 agatggggat ttactttatt atttttatta tcgccttaca gcagtttgac ggaaatgtgc
      421 ttggtcctaa aatattagga gattccacag gcttgtcggc attttgggtt gtgttttcga
      481 ttttgatcgg agggggtctg ttcggagttc ccggaatgat acttggagtt ccgacatttg
      541 cagtcgttta ttatattgtc ggaatgcttg ttaataacaa acttgaaaag aaaaaactgc
      601 ctgtcaagac agatgcttat gacgaatata gttatgtaga atcggacgga acttatgtcc
      661 atgccgataa aaatatattc aaagaaaagg agagataata ttatgcctat tcgagtacaa
      721 aacgatcttc ctgtaaaaga aatactggaa catgaaaata tatttgtgat ggatgaatat
      781 agggcggcac atcaagatat ccgtccaatc agtatcggac ttttaaatct tatgccgttg
      841 aaagaagata ccgaacttca aattttaaga tcactttcaa atacgccgct ccaagtagat
      901 gtgacgtttg ttcgtatgac gagccatgtt tctaaaaata catcaacgag tcacatttat
      961 aagttttatg aatcatttga agatattaaa atgagaaagt ttgacggatt tattattacg
     1021 ggtgcgccgg tagaacaaat ggagtttgaa gaagttgatt attggcagga attgacagaa
     1081 attatggagt ggacaaagac aaatgtgaca tcaacacttc atttgtgctg gggagcgcaa
     1141 gcgggtattt actatcatta cgggatcaat aaaaaagcgc ttccgaagaa attatccggt
     1201 gtgtacagac atcgcgtgag aaacaggaag attcctcttg taagaggttt tgatgatgtg
     1261 tttatggcgc ctcattcaag acatacggaa gttcctcagg aacttttgga gaaggatgac
     1321 agaatcatga ttcttgcaga ttctcttcag gcaggtgtat ttatgtgtat ggccaaagaa
     1381 ggg
//
LOCUS       DBMDXF010000317         1342 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_18575_length_1342_cov_1.017728, whole genome shotgun sequence.
ACCESSION   DBMDXF010000317 DBMDXF010000000
VERSION     DBMDXF010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18575_length_1342_cov_1.017728"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="ACZPD4_09095"
     CDS             <1..149
                     /locus_tag="ACZPD4_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HIR1379244.1"
                     /translation="TVTTYYLAKGLEFDQVFVVKSKKENPFEKQALYISATRALHELY
                     VYEE"
     gene            198..677
                     /gene="rlmH"
                     /locus_tag="ACZPD4_09100"
     CDS             198..677
                     /gene="rlmH"
                     /locus_tag="ACZPD4_09100"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1379245.1"
                     /translation="MKITLITVGKIKEKYLKDAIAEYSKRLSKYCKLTIVEVADEKTP
                     DQASETVEEAIRTKEGERILKVIKDDMYVITLEIGGAMLSSEELSAKIEKLGIQGKSS
                     IAFIIGGSIGLGKTVLARSDYALSFSKMTFPHQLMRVILLEQIYRSYRIINAEPYHK"
     gene            complement(689..>1342)
                     /locus_tag="ACZPD4_09105"
     CDS             complement(689..>1342)
                     /locus_tag="ACZPD4_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIR1379246.1"
                     /translation="YYNHSTLVLGYLLLFGYDASGSAYTMRLFAMLFGFILTGVVFWR
                     NHRKKTYKRGLRDLFNEFNLHSSRSRWQLLMASAVSSVIFIASILNLPRAMWAGIAAM
                     SVLLPFRTDIKKHVCGRIPGNLLGGLIFFLIFPHLPAGLYSVVGIVGGIGVGFSASYG
                     WQAVFNSLGAMMIAVSVIGFPQAVFFRIAQNIFGSLYALCFEKIFHTVTERKLESKI"
BASE COUNT          482 a          233 c          297 g          330 t
ORIGIN      
        1 tgactgtgac aacatattat ctggcgaaag gacttgaatt tgatcaagtt tttgtcgtaa
       61 aaagtaaaaa agaaaatccg tttgaaaaac aggcattgta tatcagtgcg acacgggctc
      121 ttcacgaatt gtatgtgtac gaagaataag aagaaaaaac gaaggttata ttgatttatg
      181 gggaataaac aaaaaagatg aagattacat tgatcactgt aggaaagata aaagaaaaat
      241 atttaaaaga tgcgatcgcg gaatattcaa aaagactgag taaatattgc aaattgacta
      301 tcgtagaagt tgcggatgag aaaacgccgg atcaggcgag cgaaacagta gaagaagcga
      361 tacgaacaaa agagggagaa cgtattttaa aagttataaa agatgatatg tatgtgatca
      421 ctctggaaat cggaggagca atgctttcat cggaagaact ttctgcaaag attgaaaaac
      481 tcgggataca agggaaaagc agtattgcat ttataatcgg cggttcgatc ggtcttggaa
      541 aaactgtgct tgcaagatct gattatgctc ttagtttttc caagatgacc ttcccacatc
      601 agttgatgag agtgattttg ctggaacaga tatatagaag ttatcgaatc ataaacgcag
      661 agccgtacca taaataacaa acggaaaatt atatttttga ttcaagtttt cgttctgtta
      721 cagtgtgaaa tattttttca aaacaaagag cgtacaaaga cccgaaaatg ttttgagcga
      781 ttctgaaaaa tacggcttgc ggaaaaccga tgacactgac ggcaatcatc attgctccca
      841 aagaattaaa tacagcctgc catccatagc ttgcggaaaa tccgactccg atgcctccga
      901 cgataccgac tacactgtaa agcccggcag gaagatgagg aaaaataagg aaaaagatca
      961 gtcctccgag cagattcccg ggaatacgcc cgcatacatg cttcttaata tctgttcgaa
     1021 acggaagcag gacggacatt gcggcgattc ctgcccacat cgctcttgga agatttaaga
     1081 tcgaggcgat aaaaattacg cttgaaacgg cagaagccat taaaagctgc caacggcttc
     1141 tggaagaatg aagattaaat tcgttaaaaa gatcacgcag tccgcgttta tatgttttct
     1201 ttctatgatt gcgccagaaa acgactcctg tcagtataaa tccgaataac atggcaaaaa
     1261 ggcgcattgt ataggcgctt ccggatgcat catatccaaa gagaagaaga tatccgagca
     1321 caagggttga atgattgtaa ta
//
LOCUS       DBMDXF010000318         1337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_18625_length_1337_cov_1.549353, whole genome shotgun sequence.
ACCESSION   DBMDXF010000318 DBMDXF010000000
VERSION     DBMDXF010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18625_length_1337_cov_1.549353"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="ACZPD4_09110"
     CDS             <1..224
                     /locus_tag="ACZPD4_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379247.1"
                     /translation="EDFVLNGMLFKNEEGLGFEYTQYLDMDQINWYGYISWEDLLSDE
                     NNVKVLIEPRMTSRGYPLDEDGNVIGAQE"
     gene            278..1312
                     /locus_tag="ACZPD4_09115"
     CDS             278..1312
                     /locus_tag="ACZPD4_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379248.1"
                     /translation="MKKKLLAGTVLSICLLSSGLSACSSEDKTEKPSSENQLQSDYME
                     VDGILTKTEKGSFSVHDGLMYFTDYETQTSIPVCGKPNCRHLSVYEDEKTTCNAVCSS
                     SYMFPYKEKLYRIESVDNREGKLIASHMDGSNPKTVGTFSSGDMLESAVILNENMYYT
                     YCELQNKILAEQEDGQAQSGQAMGSNCSLNRLNLDTLEQTEIVKLEDVSHILMLGGTK
                     EYQVCAVVKDDGVQYNLFDYESGTLTEISLNTVDYQRVEVTQDGKGFYYIGDEGKSLY
                     CYHIDSKENEKIVQAEEVGAEKVTVEGVSEEGILFRAFPENELYVKNEEGISNLKLSD
                     RLPTDQAWLG"
BASE COUNT          438 a          189 c          357 g          353 t
ORIGIN      
        1 aagaagactt tgttttaaac ggaatgttgt ttaaaaacga ggaaggactg ggatttgagt
       61 atacgcagta tttagatatg gatcagatta actggtatgg atacatcagt tgggaagatt
      121 tgctaagcga tgagaacaat gtaaaagtac ttatcgaacc gaggatgaca agccgaggtt
      181 atccgctgga tgaggatgga aatgtgatcg gggcacaaga atgagaatgt gagaattaac
      241 tgttaagcgt taagcgtgtg agcaggggag aacggatatg aaaaagaaac tattagcagg
      301 aacagtcttg agtatttgtc ttttatcgtc agggttgtcg gcttgttcat cagaggataa
      361 gacggaaaaa ccatcttccg agaatcagct tcaatctgat tacatggaag tagacggtat
      421 tctgacgaaa acggagaagg ggagtttttc ggtacatgac ggattgatgt attttacgga
      481 ttatgagaca cagacaagta taccggtatg cggaaagccg aactgccgcc atttatcagt
      541 atatgaagat gaaaagacga catgcaatgc agtttgttct tcttcgtata tgtttccgta
      601 taaagagaaa ctatatcgga tcgagagtgt agataatcga gaaggtaagt tgatcgcttc
      661 acatatggat gggagtaacc cgaaaacagt cggtacgttt tcttcgggag atatgttaga
      721 atcggcagtg attttaaatg agaatatgta ctatacctac tgtgaattac aaaataaaat
      781 cctggcggaa caggaagacg gacaggcgca aagcggacag gcaatgggat ctaattgttc
      841 gttgaatcgt ctgaatcttg acacgttgga gcagacggaa attgtaaagc tggaagatgt
      901 aagccatatt cttatgcttg gaggaacgaa agagtatcaa gtgtgtgctg tagtgaaaga
      961 tgacggagta caatataact tatttgatta tgagagcgga acattgactg aaatctcgtt
     1021 gaataccgtt gattatcagc gtgttgaagt gacgcaggat gggaagggtt tctattatat
     1081 cggtgatgaa ggaaagtctc tctattgcta tcatattgat tcaaaggaaa atgaaaaaat
     1141 tgttcaggcg gaagaagttg gcgctgaaaa agtcactgta gaaggtgtaa gtgaagaagg
     1201 aattttgttt cgggcatttc cggaaaatga attatatgta aaaaatgagg aaggaatcag
     1261 taatctgaag ctgtcagacc gacttccgac agatcaggcg tggcttggat aaccttattg
     1321 ccacggcccg aagcaat
//
LOCUS       DBMDXF010000319         1326 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_18804_length_1326_cov_1.641633, whole genome shotgun sequence.
ACCESSION   DBMDXF010000319 DBMDXF010000000
VERSION     DBMDXF010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18804_length_1326_cov_1.641633"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="ACZPD4_09120"
     CDS             complement(<1..262)
                     /locus_tag="ACZPD4_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379249.1"
                     /translation="MSQIEFYHNLYVSECWQKKKGKIVKRLKENKLQPQVYVITLSQG
                     EQNQLEFFSSVLLKQSIFEHSKLFVIGIADGYDEALFLVEEIV"
     gene            complement(268..>1326)
                     /locus_tag="ACZPD4_09125"
     CDS             complement(268..>1326)
                     /locus_tag="ACZPD4_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1379250.1"
                     /translation="KIQNEFKGEKDQGDTVVTTLDAELQQAAYDALGENKGAVIVMEA
                     DTGKILTLVSKPDFDPNTLAENWEILNTDEENSPLLNRATNGSYAPGSVFKIVTALAY
                     MRQDTDYNSYSYDCQGSITEDNVTIRCFNGTVHGYEDLRSSFANSCNASFANIGLSLD
                     VDGYRETAESLLFNKKLPSVMDYTKSSFVLDAKSGSAEIMMTAMGQGKTMVSPYHMAL
                     ITETIANGGTLMQPYYVDKVTNYTGTEVKKNVPKSYKRIMTSEEAAQLKEYMAAVVSE
                     GTGSVLSGRSYTVAGKTGTAEYSMTDGEKTHSWFTGFTNVDNPELVITVITEGSDGSA
                     GGKAVSIAGAVLDSYYNR"
BASE COUNT          349 a          327 c          217 g          433 t
ORIGIN      
        1 gaacaatttc ttcaacaagg aaaagcgctt catcatatcc atctgcaatt ccgatcacaa
       61 aaagctttga atgttcaaat atggattgtt ttagcaagac gcttgaaaaa aattccagtt
      121 gattttgttc tccctgagat aatgtaataa catatacttg cggctgaagt ttattttcct
      181 tcagcctttt tactattttt ccctttttct tctgccagca ttcactaaca tatagattat
      241 gataaaactc tatctgactc ataaacttca ccgattataa taagaatcca gaacagctcc
      301 tgcgatagat accgcttttc ctcctgcact tccgtcggaa ccttctgtaa taacagtgat
      361 cacaagttcc ggattgtcaa catttgtaaa tcccgtaaac catgaatgcg ttttttctcc
      421 gtctgtcata ctatattcag ccgttcctgt ttttcccgcg accgtatatg accttccgct
      481 taataccgaa ccggttcctt cacttacaac agccgccatg tactctttta actgtgccgc
      541 ttcttctgac gtcatgatcc gtttataact tttcggaaca ttctttttca cttccgtacc
      601 ggtgtaattg gtcactttat ctacataata gggttgcatc aatgtaccgc cgtttgcaat
      661 cgtctccgtg atcagcgcca tatgatacgg acttaccatt gtttttccct gtcccattgc
      721 cgtcatcata atttctgcgg aaccggactt cgcatcaagc acgaaggaac tttttgtata
      781 atccataacg cttggaagtt tcttattgaa caataagctt tccgccgttt cccgatagcc
      841 gtccacgtca aggcttaagc cgatatttgc aaatgatgca ttacatgagt ttgcaaaaga
      901 acttctcaaa tcttcgtatc catgtaccgt tccgttaaaa cagcgaattg tgacattgtc
      961 ttctgtaatg gatccctgac agtcataaga atacgagtta taatccgtat cctgccgcat
     1021 ataagcaaga gcagtcacaa ttttaaatac agaacccgga gcataagacc cgttcgtagc
     1081 ccgattcaaa agcggactgt tttcctcatc cgtatttagg atctcccagt tttcagcaag
     1141 cgtattcgga tcaaaatcag gctttgaaac aagagtaagt attttccctg tgtctgcctc
     1201 catcacaata accgctcctt tgttctctcc gagcgcatca taggcagcct gctgaagttc
     1261 tgcatcaagt gttgtaacaa ctgtgtctcc ctgatctttt tctcctttaa attcattttg
     1321 aatctt
//
LOCUS       DBMDXF010000320         1300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_19263_length_1300_cov_1.736447, whole genome shotgun sequence.
ACCESSION   DBMDXF010000320 DBMDXF010000000
VERSION     DBMDXF010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19263_length_1300_cov_1.736447"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="ACZPD4_09130"
     CDS             <1..186
                     /locus_tag="ACZPD4_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1379251.1"
                     /translation="HNQIKKYRVRLTDGTTHLLLQNEKTTGQIAQWMNSGQLETIHSC
                     EPTLETVFLDTTGRNLQ"
     gene            183..917
                     /locus_tag="ACZPD4_09135"
     CDS             183..917
                     /locus_tag="ACZPD4_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1379252.1"
                     /translation="MKITAIHFRKLSAVTEMKMRTLMSKNFIIMPIFSLGFTFLMKIL
                     YRSIADGKINMDGYALSLGVLMNISMSVYCTAAALAEEKEKNTLRTLMTSSVNGLEFF
                     LGSIIPVIILSTAVNVLCVFIVQLTLSGSQWGAYLIITTVCTFIASVVGMIFGIYAKT
                     QVSASTITTPALIIFMMVPMLSGFSSILEKISNLLFTGIALNAISAISAGKAPIHLSG
                     IIVLSVEAFLSAALFLILYRKNGFEK"
     gene            1004..>1300
                     /locus_tag="ACZPD4_09140"
     CDS             1004..>1300
                     /locus_tag="ACZPD4_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1379253.1"
                     /translation="MEIILSSTSGRPIYEQITLQIKEMIMKGDLAPGQALPSMRKLAK
                     DLHVSVITTQRAYDDLQKEGFIVTVPAKGTFVSTENQDFIREENLRRIEQLLTDA"
BASE COUNT          406 a          295 c          252 g          347 t
ORIGIN      
        1 cataatcaga tcaaaaaata ccgcgtccgc cttactgacg gcacaactca ccttctctta
       61 caaaacgaga aaacaaccgg gcaaatcgca caatggatga acagcggaca gcttgaaacg
      121 atccattcat gtgaacctac tcttgaaact gtatttttgg acacaacagg gaggaatctt
      181 caatgaaaat aacagcaatc catttcagaa aactatctgc cgtcacagaa atgaaaatga
      241 gaactctcat gagcaaaaac tttatcatca tgccgatctt ttccctcgga tttacttttc
      301 ttatgaaaat actgtaccga tccatcgctg acggaaagat caacatggac ggctacgcgc
      361 tttcgctcgg cgttctgatg aatatttcca tgagcgtata ctgtactgca gccgctcttg
      421 cggaagaaaa agaaaaaaat acgcttcgga cactgatgac ttcttcagta aacggacttg
      481 aattttttct cggaagcatt ataccggtta taattttatc cacagccgta aatgttttat
      541 gtgttttcat cgtacagctt acactttccg gatcccaatg gggcgcatat cttatcatta
      601 cgacagtctg cactttcatt gcatctgtcg tcggcatgat cttcggtatt tatgccaaaa
      661 cccaagtatc cgcaagtacg atcactacgc ctgcgctcat tatatttatg atggttccga
      721 tgttgtccgg attcagcagc attttagaaa agatctcaaa tcttctcttc accggaattg
      781 cccttaatgc tatatctgca atcagcgccg ggaaagctcc gatccatttg tccggcatca
      841 tcgtcctgtc tgtcgaagcc tttttgtcag cagcgctttt tctgatctta tatcgaaaaa
      901 acggatttga aaagtagatg caaaatacag agcatagaaa ttcaagtgca aaaccaaaat
      961 taaaacagca aaaaagccga atacgacagg gagatcaaca gatatggaaa ttattttaag
     1021 cagcacaagc ggacgtccta tctacgagca gattacgctg cagatcaaag aaatgatcat
     1081 gaaaggggat cttgcccccg ggcaggctct tccttctatg cggaaacttg caaaagatct
     1141 gcatgtcagc gttatcacaa cgcagcgtgc atatgacgat ctgcaaaaag aaggatttat
     1201 cgttactgtt cctgcaaaag gaacttttgt ttcaactgag aatcaggatt ttatcagaga
     1261 ggaaaaccta aggcgtattg aacaattact gactgacgca
//
LOCUS       DBMDXF010000321         1267 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_19857_length_1267_cov_2.038593, whole genome shotgun sequence.
ACCESSION   DBMDXF010000321 DBMDXF010000000
VERSION     DBMDXF010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19857_length_1267_cov_2.038593"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="ACZPD4_09145"
     CDS             complement(<1..1043)
                     /locus_tag="ACZPD4_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1379254.1"
                     /translation="MKKKVVSCLLAAAMVVSSGAAVPGFFFTPEKVQAEENDTKAEKE
                     NLALKGTAAASEAEADSTSAAKAIDGNKGTHWGTSQNKVTNEWIEVTLEKPTKVSEIK
                     VFWERTGAAGNHQNNIKQWKVDVKTMSGEYKNIYQKTDEDTYAPSEQTITVAEDAQEV
                     VTAVKITVNKADRGPDDFWSNIGLSEIEIYGEESDIEAAENKNHVNAAGVTAEASTTE
                     ASSLPVSNIKDGNNQTRWASDYSEASKQTVTVTFPKVTLVKELDFDLHTRDVAPMPSN
                     VKSFDLVYADAAGTEHTVKISNAKSTESGKTGYVTNVQYIFETPVYMKSFKMTNFDLQ
                     IDIEGQNGYNNIS"
BASE COUNT          279 a          337 c          206 g          445 t
ORIGIN      
        1 atactgatgt tgttatatcc gttctgtcct tcaatgtcaa tctgaagatc aaagttcgtc
       61 atcttgaagc tcttcatata aacaggcgtc tcaaaaatgt actgtacatt tgtcacatag
      121 cctgtcttgc cgctttctgt cgatttcgcg ttgctgatct taaccgtatg ctctgttcct
      181 gccgcatctg cataaacaag atcaaaggac ttcacattag acggcatcgg cgccacgtct
      241 cgagtatgaa gatcaaaatc cagctctttt acaagcgtca ccttcggaaa tgttaccgtt
      301 accgtctgct tacttgcttc cgaatagtcc gaagcccagc gcgtctggtt gttgccgtct
      361 ttaatattag aaaccggaag cgaagatgcc tctgtcgtag acgcttccgc cgtaacgcct
      421 gctgcattca catgattctt attctctgcc gcttcgatat cggactcctc tccgtaaatt
      481 tctatttcgc taagtccgat attgctccag aaatcatccg gtcctctatc cgccttgttc
      541 actgtgatct taactgcagt cacaacttcc tgcgcgtcct ctgccactgt gatcgtctgc
      601 tctgacggcg catatgtatc ttcatccgtc ttttgataaa tatttttgta ctcgccgctc
      661 atcgtcttca catcaacttt ccactgcttg atgttattct gatgatttcc ggctgctccc
      721 gttctctccc aaaacacctt gatttcgctt actttcgtcg gcttttcaag tgtcacttct
      781 atccactcgt ttgtcacttt gttctgtgat gtgccccaat gcgtaccttt atttccatca
      841 atcgccttag ccgccgatgt ggaatcagcc tctgcttcgc ttgccgcagc tgtacctttt
      901 aacgcaagat tttctttttc cgctttcgta tcattttcct cagcctgtac cttttcaggc
      961 gtaaagaaaa atcccggaac agccgctccg ctgcttacga ccatagccgc tgccaacaaa
     1021 cagctcacta ctttcttttt catacacttt ctccttttta atctttgtta cggtgattta
     1081 gacataatct atgtttttca caaaacaccg agcataatca tattcattat aacaccttta
     1141 catttttcag gtatatttta tttacacaaa atataatttt tgtatacttt ttctatttta
     1201 aatcatattt tttgtgcatt tcgacaattc aaaaggggat tttctttgct tccctttcgc
     1261 cattaag
//
LOCUS       DBMDXF010000322         1240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_20375_length_1240_cov_2.033363, whole genome shotgun sequence.
ACCESSION   DBMDXF010000322 DBMDXF010000000
VERSION     DBMDXF010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20375_length_1240_cov_2.033363"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /gene="gltX"
                     /locus_tag="ACZPD4_09150"
     CDS             complement(<1..895)
                     /gene="gltX"
                     /locus_tag="ACZPD4_09150"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1379255.1"
                     /translation="MENKVRTRFAPSPTGRMHVGNLRTALYAYLIAKHEGGDFILRVE
                     DTDQERFVEGALDIIYRTMEKTGLLHDEGPDKDGGFGPYVQSERQASGLYLDYAKQLV
                     EQGDAYYCFCDKERLESLKTSVSDDGTDIVVYDKHCLGLSKEEVEKNLAEGKPWVIRL
                     NVPNEGETTFHDEIYGNITVPNNELDDMILIKSDGFPTYNFANVIDDHLMGITHVVRG
                     NEYLSSAPKYNRLYEAFGWEIPVYVHCPLITDENHKKLSKRCGHASYEDLIDQGFVSE
                     AVVNYVALLGWCPSDNREIFSL"
     gene            complement(1056..>1240)
                     /locus_tag="ACZPD4_09155"
     CDS             complement(1056..>1240)
                     /locus_tag="ACZPD4_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379256.1"
                     /translation="SICGADYNFVSARTGNYKVPSHVTEIADGAFDRAGISVLDASDC
                     SVLEIRSLPSYIELRR"
BASE COUNT          341 a          316 c          198 g          385 t
ORIGIN      
        1 ccaaagaaaa gatctctctg ttgtcagaag gacaccatcc aagcaaagcc acataattca
       61 caaccgcttc agacacaaat ccctgatcga tcaaatcctc ataagaagca tgaccgcatc
      121 ttttacttaa ttttttatgg ttttcatctg tgataagcgg acagtgtaca taaaccggaa
      181 tttcccatcc aaacgcctca tagagacgat tgtatttcgg cgcggacgaa agatattcat
      241 tcccacgtac gacatgtgtg atccccataa gatgatcgtc tattacattt gcaaagttat
      301 atgtcggaaa tccatcggat ttaatcagga tcatatcgtc caactcattg ttcggcactg
      361 taatattccc gtaaatctcg tcatggaatg ttgtctctcc ctcattcgga acattcagac
      421 ggatcaccca tggttttcct tccgcaaggt ttttctccac ttcttcttta ctaaggccga
      481 gacaatgctt gtcataaaca acaatatctg ttccgtcgtc agaaaccgat gttttcagac
      541 tttcaagacg ctctttatca cagaagcagt aataagcgtc tccctgttct acaagctgtt
      601 tcgcataatc cagataaagt ccggatgcct gtctttcact ttgaacgtac ggaccgaatc
      661 ctccgtcttt atcaggtcct tcatcatgaa gaagtcctgt tttttccatt gttctgtata
      721 tgatatcaag cgcgccttca acaaaacgct cctggtctgt atcttctact cttaaaataa
      781 aatctccgcc ttcatgcttt gcaattaaat atgcataaag cgctgttctt aaattcccca
      841 catgcattct tccggtcgga ctgggcgcaa atcttgttct tactttattt tccatgtttt
      901 ttctccactt ctatttcttc tattttcctg ctctgtgcag tgtcccggct ctggccgttt
      961 catttttctg tcacagaacc gtcttctgta taaaacttac aggcaacatt ataaaacaac
     1021 tgcaaaaaat gttcaagacg ctgtatactt tcttttcacc gtctcagttc aatataagac
     1081 ggcaaactcc tgatttctaa aacggaacaa tcagaagcat ccaagacgga aattcccgcc
     1141 cggtcaaacg ctccgtccgc aatttctgtc acatgtgaag gaactttata atttcccgtt
     1201 cttgccgata caaaattata atccgcccca caaatagaaa
//
LOCUS       DBMDXF010000323         1211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_20982_length_1211_cov_1.760360, whole genome shotgun sequence.
ACCESSION   DBMDXF010000323 DBMDXF010000000
VERSION     DBMDXF010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20982_length_1211_cov_1.760360"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="ACZPD4_09160"
     CDS             <1..1109
                     /locus_tag="ACZPD4_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1379257.1"
                     /translation="ASIVGIFCNVFLFAVKFTIGVIAHSVSVMADAFNNLSDAGSSII
                     SFIGVKMAEKPADRDHPFGHGRIEYIAALVVSFLILEVGFTFLKDSIGKIRTPEMLNF
                     QIASVIILILSIGVKLWLGLFNKRLGEKINSKVMMAVFADSMGDVITTGATILSLIVF
                     RITGWNIDGVVGIGVALVVMWAGVGIAKDTLEPLIGEAIDPQVYDQIKAFVEKYDGIV
                     GTHDLIVHNYGPGRSMASIHAEVPNDVDIEKSHEIIDRIERDAKQTLGLFLVIHMDPV
                     EMKDENVLKIRKVAEKILRELDPSCSLHDFRIVHGTRQINLVFDMVIPINYDEERRNE
                     LPLLMMERLKQIDNRYECVITVDYEFVAKAEAEE"
BASE COUNT          363 a          202 c          335 g          311 t
ORIGIN      
        1 tggcaagtat tgtaggtatc ttctgtaatg tctttttgtt tgcagtaaaa tttactatcg
       61 gagtgatcgc gcacagcgtg tcggttatgg cggacgcttt caacaatctg tcggacgcag
      121 gttcgtcgat cattagtttt atcggagtga aaatggcgga gaaacctgcg gacagagatc
      181 atccgttcgg acatggaagg attgaataca tagcggcgct agtagtttct tttcttattt
      241 tggaagtagg gtttactttt ctgaaagatt cgatcgggaa aatacgaacg ccggaaatgc
      301 tgaattttca gatagcttcg gtcattatac tgatcttatc gatcggagtg aaactgtggc
      361 ttggtctgtt caacaagcgg cttggcgaga agataaattc taaagtgatg atggcagtgt
      421 ttgcggattc tatgggagat gtgatcacaa caggcgcgac gatcctttcg cttatcgttt
      481 ttcgaattac gggctggaac attgacggag tggtcggaat cggagttgcc cttgttgtaa
      541 tgtgggcagg agtcggcatt gcaaaagata cgttggaacc gctgatcggt gaagcaatcg
      601 atcctcaggt atatgatcag attaaagctt ttgtagaaaa atacgacggc attgtaggaa
      661 cgcatgatct gatcgttcat aattacggac cgggaaggag catggcttcg atccatgcgg
      721 aagtaccgaa tgacgtagat atcgaaaaat cccatgagat catcgaccgg atcgagagag
      781 atgccaagca gacgttaggt ctgtttcttg tcattcatat ggatccggtg gagatgaaag
      841 atgaaaatgt gctcaagata cggaaagtgg cggaaaagat cttaagagaa ctcgatcctt
      901 cgtgcagtct gcatgatttt cgtatcgttc acggaacaag acagatcaat cttgtattcg
      961 atatggtcat tcccatcaat tatgatgagg agagaagaaa cgagcttccg ctgctgatga
     1021 tggagaggct gaaacagatc gacaacagat atgagtgcgt gataacagtc gattatgaat
     1081 ttgtggcgaa agcggaagca gaagagtaga gcgaaggata aaagacggat gaaagaagaa
     1141 taaaggacga tcggaaaatg ctttaaaaga ggcggaaaag cgaaaaacag ctgcaagaag
     1201 agcgtgaaaa a
//
LOCUS       DBMDXF010000324         1189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_21453_length_1189_cov_1.715993, whole genome shotgun sequence.
ACCESSION   DBMDXF010000324 DBMDXF010000000
VERSION     DBMDXF010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21453_length_1189_cov_1.715993"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="ACZPD4_09165"
     CDS             <1..944
                     /locus_tag="ACZPD4_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1379258.1"
                     /translation="TELMENAVEGYNDQIRIIDKHEIETKFDFGGSAKDDSLTKKPFT
                     VYISGIDTYGDDADDDRDSIRSDVNIIAVVNPTTHQILLITTPRDYYIPIPGISDGMN
                     DKLTHAGTYGIDVSMETLGALYETDINYYVRLNFSSLIEIVDILGGVDVYSEYAFTTG
                     WESGYEMEVQQGINHFDGKQALAFSRERHALEGGDNQRGKNQQAVITAMLKKVLSPTM
                     LLKANSIINQVSQDVETNISQGQLNSLVKSQLKSGAKWTIQSVAASGTDGEDYCYSVP
                     DMPLYVLYPDEEVVNSIVELANIVEEGGSLESGEALN"
BASE COUNT          395 a          177 c          304 g          313 t
ORIGIN      
        1 atacagaatt gatggaaaat gctgttgaag gctacaacga tcagatccgg atcattgata
       61 agcacgagat tgaaacgaag tttgatttcg gaggttcggc aaaagacgac agtttgacta
      121 aaaagccatt tactgtgtat attagcggaa ttgatacata cggagacgat gcggacgatg
      181 accgtgattc catacgaagt gatgtgaata tcattgcagt cgtaaatccg acgacgcatc
      241 agattttgct gattacgaca ccgcgagatt attatattcc gatacccgga atatccgatg
      301 gaatgaacga taagctgaca catgccggaa catatggaat agatgtgtcc atggaaacgc
      361 tcggtgcgtt gtatgaaacg gatattaact attatgtaag attaaacttc agttccctta
      421 ttgagattgt agatatctta ggaggagtag atgtttactc ggaatatgca tttacgacag
      481 gatgggaatc cggttatgag atggaagtac agcaaggaat aaatcatttt gacggaaaac
      541 aagcgcttgc tttcagcagg gaacggcatg cattggaagg cggagacaat caaagaggta
      601 agaaccaaca ggctgttata acagctatgc ttaaaaaagt gctttcaccg acgatgcttt
      661 tgaaagcaaa ttcgatcatt aatcaagtga gtcaggatgt agagacaaac atttcacagg
      721 gacagctgaa ttctcttgtg aaaagccagc ttaagtcagg ggcaaaatgg acaattcagt
      781 ctgtggcggc aagcggaaca gatggggagg attattgcta ctctgtgccg gacatgcctt
      841 tatatgtgct ctatcctgat gaagaagttg tgaacagtat tgttgaattg gcgaatatcg
      901 tggaagaagg cggaagtctt gaaagcggag aggcgttgaa ttaatactga ttataaagaa
      961 tgcatagaaa taacgttact gttatatttg aattataaaa gaaatagatg aaaagcaaag
     1021 ctgtttacag gcgtggaata gataactgta gaaagctctg ttttttgtta tcttgagtac
     1081 gtggaaagaa attatgaaat gatatgtaaa tagtatggcg ctaaaaagtg gtggtagtgt
     1141 tcggcgaaat ggattgggag aaaggttgga cacaacagtt ccattatgg
//
LOCUS       DBMDXF010000325         1146 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_22460_length_1146_cov_1.645933, whole genome shotgun sequence.
ACCESSION   DBMDXF010000325 DBMDXF010000000
VERSION     DBMDXF010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22460_length_1146_cov_1.645933"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /locus_tag="ACZPD4_09170"
     CDS             complement(<1..>1146)
                     /locus_tag="ACZPD4_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1379259.1"
                     /translation="DKLTLGTSLNAYEWTTYDAKKPGDARYIRLINKTNDDVKFNLKQ
                     FVVNTVEFKEKSLHSKSGNYTITDPEKAFDGNRATEAVYQTSQNEGVWFVYDLGQTIH
                     FDKFKAVCEDSEHDYIRHGKFSVSTDGETWEEIMTLGSQDGPNKGEEDNTDEIGAVLP
                     NHETSYNTKEATNINKDARYLKFEVTRTKVGSDKWVRFSELEINDGAYVPSENDPTYE
                     SSCLDTQNGKFSYMSDMNLATSFVPAEKSGNLVYHVSEKNDRNKIKIVQRADSISNAT
                     VSVRTLAEADTWKPVGTLGKTVNEFVLPNETVLLDVKIEWSDAKVGIIEMFLSKTDAP
                     TPEVKEWTVTLDGKEVKVKDGEKLTKPADPTKEGYKFLGWFVGDKEF"
BASE COUNT          257 a          273 c          201 g          415 t
ORIGIN      
        1 ttaaactctt tatctcctac aaaccatccg aggaatttgt atccctcttt tgtcggatct
       61 gccggtttcg tcagtttctc tccgtctttt acttttactt cttttccgtc aagtgtcact
      121 gtccattctt ttacttccgg tgtcggagca tctgtttttg aaaggaacat ctctataatg
      181 ccgaccttag catctgacca ctcaattttc acatcaagaa gcactgtctc attcggaaga
      241 acaaactcat tcactgtctt gcccagtgtt ccgacaggtt tccatgtatc tgcttctgct
      301 aatgttctca cacttacagt tgcatttgaa atcgaatctg ctctctgaac aatcttaatc
      361 ttattgcggt cattcttctc tgatacatga taaacaaggt ttccgctctt ttctgccgga
      421 acaaatgatg tcgcaagatt catatctgac atataagaga attttccgtt ttgtgtatcg
      481 agacatgagc tttcatatgt cggatcgttt tcgctcggaa catacgctcc gtcattgatc
      541 tcaagctcgc tgaagcgaac ccacttgtca gaaccgactt ttgttcttgt cacctcgaat
      601 ttcaggtaac gtgcatcttt gttaatattt gttgcttctt ttgtattata agaagtttca
      661 tgattcggaa gtacagcgcc gatctcgtct gtattgtcct cttctccttt attcggacca
      721 tcctgcgaac caagcgtcat gatttcttcc catgtctctc cgtctgtaga tacagagaat
      781 ttaccatgtc tgatatagtc atgctcgcta tcctcgcaga cagctttgaa cttatcgaag
      841 tggattgtct gtccgagatc gtatacgaac caaacgcctt cgttctgtga tgtctgataa
      901 acagcctctg ttgctctgtt tccgtcgaac gctttctcag gatctgtgat cgtataattt
      961 ccgctttttg aatgcagtga tttttctttg aactctaccg tatttacaac gaattgtttc
     1021 aaattgaatt tcacatcatc attcgtcttg ttgatcagtc tgatatatct tgcatctccc
     1081 ggtttctttg catcatatgt tgtccactca tatgcattga gagatgttcc aagggtaagc
     1141 ttgtct
//
LOCUS       DBMDXF010000326         1113 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_23298_length_1113_cov_2.991107, whole genome shotgun sequence.
ACCESSION   DBMDXF010000326 DBMDXF010000000
VERSION     DBMDXF010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23298_length_1113_cov_2.991107"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            120..1013
                     /locus_tag="ACZPD4_09175"
     CDS             120..1013
                     /locus_tag="ACZPD4_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1379260.1"
                     /translation="MFQVFVDSAANLPAVTAKKYDIHVVSFVNLVNGKELVCFDPDLS
                     PEEELSKGKEYYNAMRTGCEIKTGLISTANFADAFRTALEADQDVIYLSLSKNISGTY
                     NSARLAAEELLEEYGGKRKIRLVDSLNASLAQGILAIYASEMRDQGMNVDEAADLLET
                     YVGKMNGVFTVGDLKYLSRTGRIKGSVAVVGNMLKIKPILRGSEDGYIVSYKNVRGRK
                     AVLNELIRLFCETIEEPEKQIVGIAHADAYEESLYMMEEIQKRVKVREFINTSYDYCT
                     GSHVGPDTIALFYLGKDRSLL"
BASE COUNT          310 a          245 c          254 g          304 t
ORIGIN      
        1 gtagtatgtt gtctatcttc cattgaaatc tctcttcttt ggtgatacaa tgtgctacaa
       61 tgcttatcaa aagttacatc attttggggg acatttacac tacaaaggag aatttattta
      121 tgtttcaggt ttttgttgat tcagcagcaa atctgcctgc tgttactgcg aaaaaatacg
      181 atatccatgt cgtttctttc gtcaatcttg tgaatggaaa agagctggtc tgcttcgacc
      241 cggatttgtc gccggaagag gagctttcca aaggaaaaga atattacaat gctatgcgta
      301 ccggctgtga aatcaagacc ggactgatca gcaccgcaaa ttttgcggat gcgttccgca
      361 ccgctcttga agcagatcag gatgtcatct atctttcctt aagcaaaaac atcagcggaa
      421 cttataactc cgcaagactt gctgccgagg agcttcttga ggaatacggc ggaaaacgta
      481 aaatccgcct cgtcgattcc ctgaatgcct ctcttgcaca gggcatcctc gccatctatg
      541 ccagcgagat gcgcgatcag ggcatgaacg tagacgaagc ggcagatctt ctggaaacct
      601 atgttggcaa aatgaacggt gttttcaccg tcggcgatct gaaatatctt tcccgtaccg
      661 gaagaatcaa gggaagtgtc gctgtggtcg gaaatatgct gaaaatcaaa ccgattctgc
      721 gtggaagtga agatggctat attgtttcct acaagaatgt ccgcgggcgc aaggccgttt
      781 taaatgaact gatccgtctt ttctgtgaaa cgatcgaaga accggaaaag cagatcgtcg
      841 gaatcgcaca tgcagatgcc tatgaggaat ctctttatat gatggaagaa atccagaaaa
      901 gagtgaaggt gcgtgaattt atcaatactt cttacgatta ctgcaccgga agccatgtcg
      961 gaccggatac gatcgctttg ttctatctcg gaaaagaccg cagccttctg taaaaggagc
     1021 aaccagacta accactgctg ttccactgta tttttccgaa attgctgcaa acagcggaaa
     1081 tactgtttct tttttccatg cataattcca cct
//
LOCUS       DBMDXF010000327         1099 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_23664_length_1099_cov_2.047094, whole genome shotgun sequence.
ACCESSION   DBMDXF010000327 DBMDXF010000000
VERSION     DBMDXF010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23664_length_1099_cov_2.047094"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(79..222)
                     /locus_tag="ACZPD4_09180"
     CDS             complement(79..222)
                     /locus_tag="ACZPD4_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1379261.1"
                     /translation="MGLGLDGMQKMNGAINVDFMTEEEIHQKLEAGYKDMESGKVREA
                     SIV"
     gene            complement(598..>1099)
                     /locus_tag="ACZPD4_09185"
     CDS             complement(598..>1099)
                     /locus_tag="ACZPD4_09185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIR1379262.1"
                     /translation="IIVNKEHLEELLRELRLKTPDEIKRYQKIIANRDAILADAQAKA
                     DAMIEEAQVQTTELVSEHEIMQQAYAQANEIVTQATAQAQEIIDNATSDANAIRIGAI
                     QYTDDLMANAESIIGHTLDSYTTKYDGLISSLQECYETVRANRAELDIPTEEESGIYN
                     SQDDLF"
BASE COUNT          271 a          271 c          179 g          378 t
ORIGIN      
        1 acccgatatt gcttataaaa tcccaaagcg gttgcttttt ttgcaaagtc tgttacttgt
       61 tctatcgttt gctttgtatc atacaatcga tgcctcccga acttttccgc tttccatatc
      121 cttatatccc gcttctagct tctgatgaat ctcttcctca gtcatgaaat caacattaat
      181 cgcgccgttc attttctgca ttccatctaa acctaaaccc atgcactctg tttgttcttt
      241 ctgatctctg gaacacctcc ccgtccttct gaaacgattg taacgctttt tcagacaaat
      301 gaacagagtt cttctatttt taatacttct tgaaaatgta tttcagatga atggacgcta
      361 catccagata actgcaaaag ttcccatttt tgaacacttt ccgacactag atctgtcaca
      421 tttcgaaaag atgtcctccg gaagtcgaac atgtgcacat cggctttcaa taccgctgct
      481 ttccggtaca aaagatgcct tccaaacaat cttgcttgaa aggcatctct tttttcttaa
      541 aagcgtctgc ttttattgta tcatatataa ttaaaaatag tcgaaatatc cgtcacccta
      601 gaataaatcg tcctgagaat tatatattcc ggactcttct tctgttggga tatcaagttc
      661 tgcgcgattt gcgcgaacgg tttcataaca ttcctggagg gagctgatca gaccatcgta
      721 ttttgttgta taactatcaa gtgtatgtcc gataatactt tcggcatttg ccataagatc
      781 atctgtatac tggattgctc cgatcctgat tgcatttgca tcactcgttg cgttatctat
      841 aatctcctga gcctgtgccg tagcctgcgt tacaatctca tttgcctgcg cataagcctg
      901 ctgcatgatc tcatgctcgc tgacaagctc tgtcgtctgt acctgcgctt cttcaatcat
      961 cgcatccgcc tttgcctgtg catccgcaag aatcgcatct ctgtttgcga taatcttctg
     1021 ataacgttta atctcgtcag gagttttcag acgaagttct ctcaaaagct cttctaaatg
     1081 ttctttattc acaataatc
//
LOCUS       DBMDXF010000328         1050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_25018_length_1050_cov_1.166491, whole genome shotgun sequence.
ACCESSION   DBMDXF010000328 DBMDXF010000000
VERSION     DBMDXF010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25018_length_1050_cov_1.166491"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="ACZPD4_09190"
     CDS             <1..389
                     /locus_tag="ACZPD4_09190"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1379263.1"
                     /translation="WKFISRIRQNTKNLPEVTLYTSLGVKKVIGLIDTGNELRDFASD
                     DPVNIIDPRLAESITTHPEWEKGFHMIPYSSIGGDGVMKVFRIEKMCIHMDEDIWIEN
                     PLLGMSERTLSEKERYEMILNPGIFL"
     gene            483..>1050
                     /locus_tag="ACZPD4_09195"
     CDS             483..>1050
                     /locus_tag="ACZPD4_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1379264.1"
                     /translation="MMMKVAVPQRFKLRVVPDVKTLIFGSGREVHYIGGTEVLPPPLE
                     GMRESEMINRLGTEYAEDAKKVLIEHNLRLVVYIAKKFDNTGVGVEDLISIGTIGLIK
                     AINTFNPTKKIKLATYASRCIENEILMYLRRNSKTKMEVSIDEPLNVDWDGNELLLSD
                     ILGTEEDTIYKDLENEAERKLLIRALNKL"
BASE COUNT          352 a          155 c          267 g          276 t
ORIGIN      
        1 tgtggaagtt tatatcgcgc ataagacaga atacgaaaaa tcttccggaa gtaacattgt
       61 atacaagtct tggggtaaag aaagtgatcg gtcttataga tacgggaaat gagctccgcg
      121 attttgcgtc tgatgatccg gtcaatatta ttgatccgcg attggcagag tctattacga
      181 cacatccgga atgggaaaaa ggctttcata tgattccgta cagcagcatc gggggagatg
      241 gagtgatgaa ggtgtttcgg attgaaaaaa tgtgtattca catggatgaa gacatatgga
      301 tagaaaatcc gcttctcggg atgagtgaaa gaactctttc ggaaaaagaa cgatatgaaa
      361 tgatcctgaa tccggggatc tttttataag cgggagttaa aaaatatgga ttaatatctt
      421 tgacagttta tatttactgc cgcttaaaat tactaaaaca ggggaatata ggaggatatg
      481 acatgatgat gaaagtagct gttccgcagc ggtttaagtt aagagtagta cctgatgtga
      541 aaaccttgat tttcgggagc gggagagagg tgcattatat aggaggaacg gaagttcttc
      601 cgccgcctct tgaggggatg agagaaagcg agatgattaa ccggctcgga acagagtatg
      661 cagaggatgc aaaaaaagta ttaatagaac acaatctccg gcttgttgta tacattgcga
      721 aaaaatttga caatacaggc gtaggcgttg aagatttgat ttcgatcgga acgatcggtt
      781 tgatcaaagc aatcaatacg tttaatccga caaaaaagat caaacttgca acatacgcat
      841 cccgatgtat tgaaaatgaa attttaatgt acttaaggcg taatagcaag acaaagatgg
      901 aggtgtccat cgacgaacct ttgaacgttg actgggatgg aaacgagctg cttttgtcgg
      961 atattttggg aacggaagaa gatacgattt ataaagatct ggaaaatgaa gcggagagaa
     1021 aacttttgat tcgtgctctg aataaactta
//
LOCUS       DBMDXF010000329         1025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_25808_length_1025_cov_1.625541, whole genome shotgun sequence.
ACCESSION   DBMDXF010000329 DBMDXF010000000
VERSION     DBMDXF010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25808_length_1025_cov_1.625541"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="ACZPD4_09200"
     CDS             <1..297
                     /locus_tag="ACZPD4_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1379265.1"
                     /translation="KAKVITLTDSVHSPMNLYSSCNLIARSDMASIVDSLVAPLSVVN
                     ALVVALCMKRQKEVVSTLETLEEIWGEYEVYSRDELDPMKRSFFPDETAGGKDE"
     gene            290..>1025
                     /locus_tag="ACZPD4_09205"
     CDS             290..>1025
                     /locus_tag="ACZPD4_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HIR1379266.1"
                     /translation="MSKVIVVGGGAAGMMAAVFAAENGHHVQIYEKNDRLGRKLFITG
                     KGRCNITNASDVEELFQTVVSNPKFLYSAFYSFTNEQVIDFFENLGVKTKVERGNRVF
                     PVSDHSSDIIGALQRELHRLGVQISLCTEVEKICVENEKITGIIISDDSQKQKQHFVD
                     ADAVIIATGGISYPATGSTGDGYRFAKTCGHKVTELFPALVPMEVKEWYAKQLQGLSL
                     RNVCIRVTDGKRKLYEEFGEMLFTHYG"
BASE COUNT          320 a          170 c          268 g          267 t
ORIGIN      
        1 aaggcaaaag tgatcacttt aacggacagc gtccattcgc ctatgaatct gtattcatcg
       61 tgcaatctga ttgcaagaag tgatatggcg tcgatcgtag actctcttgt agcgccgtta
      121 agcgtagtga atgctctcgt agtggcgctt tgcatgaaga gacaaaaaga agttgtatca
      181 acacttgaga cgttagagga aatatgggga gaatatgaag tgtacagcag agatgaatta
      241 gatcctatga aacgttcgtt tttcccggat gaaacggcag gaggaaaaga tgagtaaagt
      301 gatagttgtc ggaggcggag ccgcaggaat gatggcggct gtatttgccg ccgaaaacgg
      361 gcatcatgtt cagatttatg agaaaaatga cagattgggc aggaaattat ttataacggg
      421 aaagggacgc tgtaatatta caaatgcatc ggatgttgaa gaattatttc agacggtagt
      481 cagtaaccca aagtttttat atagtgcatt ttacagtttt acgaatgaac aggtgatcga
      541 cttttttgaa aatctcggtg tgaagacgaa ggttgaaaga ggaaacagag tctttcctgt
      601 ttccgatcat tcgtcggata taatcggggc gcttcaaaga gaactgcacc gtctcggcgt
      661 tcagatatct ctttgtacgg aagtggaaaa aatatgtgta gaaaacgaga aaataacagg
      721 aatcattatt tctgatgatt cccaaaaaca aaagcagcat tttgttgacg cagatgcggt
      781 gatcatagcg actggaggga tttcttatcc tgctacagga tcgacaggag acggataccg
      841 atttgcaaaa acatgcgggc ataaggttac cgaacttttt ccggctctcg ttccgatgga
      901 agtaaaagaa tggtatgcaa aacagcttca gggtctgtcg cttcggaacg tatgtatccg
      961 tgtgaccgac ggaaaaagga aattatatga agaattcgga gagatgctgt ttacccatta
     1021 tggag
//
LOCUS       DBMDXF010000330         1012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Mediterraneibacter torques isolate MAG1185
            NODE_26229_length_1012_cov_1.304061, whole genome shotgun sequence.
ACCESSION   DBMDXF010000330 DBMDXF010000000
VERSION     DBMDXF010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508284
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mediterraneibacter torques (human gut metagenome)
  ORGANISM  Mediterraneibacter torques
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Mediterraneibacter.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.8573x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,842
            CDSs (total)                      :: 1,810
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 22 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Mediterraneibacter torques"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26229_length_1012_cov_1.304061"
                     /isolate="MAG1185"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:33039"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1012
                     /locus_tag="ACZPD4_09210"
     CDS             <1..>1012
                     /locus_tag="ACZPD4_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1379267.1"
                     /translation="AYDAFLGKEIDYAAIEASGGTKMVKPSQQAEADIKFGYCTEFII
                     MTEKEFTDKNEAEFKAYLESIGDSIVCVADDDIVKIHVHTNDPGLAIQKALTYGQLSR
                     MKIDNMREEHQEKLIKDAEKAAAQQASEKKKKEPRKSVGFIAVSIGEGMNEIFRELGA
                     DYIIEGGQTMNPSTDDMLSAIDEVNADHIFILPNNKNIILAANQAQSLTDDKDIIVIP
                     TKTVPQGITAIISYMPEADVDTNIEALEEAIKNVKTGQVTYAVRDTHIDDKEIHEGDI
                     MGIGDRGILAVGQSVEETTKEMLAQLVDEESELISLYFGQDVLEEDAENFAQEVEELY
                     PDV"
BASE COUNT          355 a          171 c          252 g          234 t
ORIGIN      
        1 gcgtatgatg catttcttgg aaaagaaata gattatgcgg caatcgaagc aagcggcgga
       61 acaaaaatgg taaaaccttc ccagcaggcg gaagcggata ttaagtttgg ctattgtaca
      121 gaatttatta tcatgacaga aaaagaattt acagataaga atgaagcaga gtttaaagct
      181 tatcttgaat cgatcggcga ttcgatcgtt tgtgttgccg atgatgatat tgtgaaaatt
      241 catgtacaca cgaacgatcc gggacttgcg attcaaaaag cgctcacata cggtcagctt
      301 tccagaatga agattgataa catgagggaa gagcatcagg aaaaactgat caaagacgca
      361 gaaaaagcgg cagctcaaca ggcatctgag aagaagaaaa aagaaccgag aaagtcggtt
      421 ggttttatag ccgtatctat cggtgaagga atgaatgaga ttttcagaga gctcggtgca
      481 gattatatca ttgaaggcgg acagacaatg aatccgagta cagatgatat gctcagtgcg
      541 atcgatgaag tgaatgcaga ccatatcttt attcttccga acaataaaaa cattatcctt
      601 gcggcgaatc aggcacagtc attaacagat gacaaagata ttattgttat tccgacaaag
      661 actgttccac agggaattac cgcaattatc agttacatgc cggaagcgga cgtagataca
      721 aatattgaag cgttagaaga agcaatcaaa aacgtaaaga ccggacaggt cacatatgca
      781 gtccgtgata cgcacattga tgacaaagaa atacatgaag gcgatataat gggaatcgga
      841 gatcggggaa ttctcgcagt cggacagtct gttgaagaga cgacaaaaga gatgcttgca
      901 caactcgtcg atgaagaatc cgaattgatc agcctgtatt tcggacagga cgttttggaa
      961 gaagatgcgg aaaattttgc gcaggaagtg gaagagttgt atcctgatgt ag
//
